LOCUS       DAVKQB010000001        76508 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKQB010000001 DAVKQB010000000
VERSION     DAVKQB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 76508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.001"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(513..1709)
                     /gene="tuf"
                     /locus_tag="VW528_000001"
     CDS             complement(513..1709)
                     /gene="tuf"
                     /locus_tag="VW528_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9910904.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2620..3489)
                     /locus_tag="VW528_000002"
     CDS             complement(2620..3489)
                     /locus_tag="VW528_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9910905.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3713..4321)
                     /locus_tag="VW528_000003"
     CDS             complement(3713..4321)
                     /locus_tag="VW528_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9910906.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKTETPVNPDPEVPTYE
                     TGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            complement(4279..5385)
                     /locus_tag="VW528_000004"
     CDS             complement(4279..5385)
                     /locus_tag="VW528_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9910907.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGRQEKLVESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5496..5750)
                     /locus_tag="VW528_000005"
     CDS             complement(5496..5750)
                     /locus_tag="VW528_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9910908.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(6090..6536)
                     /locus_tag="VW528_000006"
     CDS             complement(6090..6536)
                     /locus_tag="VW528_000006"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9910909.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERISIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLECFFKTR"
     gene            complement(6609..6934)
                     /locus_tag="VW528_000007"
                     /pseudo
     CDS             complement(6609..6934)
                     /locus_tag="VW528_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7168..8886
                     /locus_tag="VW528_000008"
     CDS             7168..8886
                     /locus_tag="VW528_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9910910.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            8997..9344
                     /locus_tag="VW528_000009"
     CDS             8997..9344
                     /locus_tag="VW528_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9910911.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFVGL"
     gene            complement(9623..10459)
                     /locus_tag="VW528_000010"
     CDS             complement(9623..10459)
                     /locus_tag="VW528_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9910912.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10472..11101)
                     /locus_tag="VW528_000011"
     CDS             complement(10472..11101)
                     /locus_tag="VW528_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9910913.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(11111..11752)
                     /locus_tag="VW528_000012"
     CDS             complement(11111..11752)
                     /locus_tag="VW528_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9910914.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11898..13127)
                     /locus_tag="VW528_000013"
     CDS             complement(11898..13127)
                     /locus_tag="VW528_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9910915.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(13117..14283)
                     /locus_tag="VW528_000014"
     CDS             complement(13117..14283)
                     /locus_tag="VW528_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9910916.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFIAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14427..15440
                     /locus_tag="VW528_000015"
     CDS             14427..15440
                     /locus_tag="VW528_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9910917.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15485..15904)
                     /locus_tag="VW528_000016"
     CDS             complement(15485..15904)
                     /locus_tag="VW528_000016"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9910918.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15915..17321)
                     /gene="atpD"
                     /locus_tag="VW528_000017"
     CDS             complement(15915..17321)
                     /gene="atpD"
                     /locus_tag="VW528_000017"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9910919.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17407..18285)
                     /locus_tag="VW528_000018"
     CDS             complement(17407..18285)
                     /locus_tag="VW528_000018"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9910920.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(18301..19806)
                     /locus_tag="VW528_000019"
     CDS             complement(18301..19806)
                     /locus_tag="VW528_000019"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9910921.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19821..20357)
                     /locus_tag="VW528_000020"
     CDS             complement(19821..20357)
                     /locus_tag="VW528_000020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9910922.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(20357..20851)
                     /gene="atpF"
                     /locus_tag="VW528_000021"
     CDS             complement(20357..20851)
                     /gene="atpF"
                     /locus_tag="VW528_000021"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9910923.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20865..21581)
                     /gene="atpB"
                     /locus_tag="VW528_000022"
     CDS             complement(20865..21581)
                     /gene="atpB"
                     /locus_tag="VW528_000022"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9910924.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNILEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21616..21816)
                     /locus_tag="VW528_000023"
     CDS             complement(21616..21816)
                     /locus_tag="VW528_000023"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9910925.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(21891..22898)
                     /locus_tag="VW528_000024"
                     /pseudo
     CDS             complement(21891..22898)
                     /locus_tag="VW528_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(23319..23717)
                     /locus_tag="VW528_000025"
     CDS             complement(23319..23717)
                     /locus_tag="VW528_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9910926.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAL
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(23999..24481)
                     /gene="greA"
                     /locus_tag="VW528_000026"
     CDS             complement(23999..24481)
                     /gene="greA"
                     /locus_tag="VW528_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9910927.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(24552..26207)
                     /gene="mltG"
                     /locus_tag="VW528_000027"
     CDS             complement(24552..26207)
                     /gene="mltG"
                     /locus_tag="VW528_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9910928.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGSETPTPATETVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(26288..26782)
                     /locus_tag="VW528_000028"
     CDS             complement(26288..26782)
                     /locus_tag="VW528_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9910929.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(26779..27243)
                     /locus_tag="VW528_000029"
     CDS             complement(26779..27243)
                     /locus_tag="VW528_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9910930.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(27253..28587)
                     /locus_tag="VW528_000030"
     CDS             complement(27253..28587)
                     /locus_tag="VW528_000030"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9910931.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEVEGNDFVASDLLRSITGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(28598..29215)
                     /locus_tag="VW528_000031"
     CDS             complement(28598..29215)
                     /locus_tag="VW528_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9910932.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVQPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(29263..32361)
                     /locus_tag="VW528_000032"
     CDS             complement(29263..32361)
                     /locus_tag="VW528_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9910933.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVVVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(32454..33620)
                     /locus_tag="VW528_000033"
     CDS             complement(32454..33620)
                     /locus_tag="VW528_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9910934.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVVTFAKR"
     gene            complement(33628..34722)
                     /locus_tag="VW528_000034"
     CDS             complement(33628..34722)
                     /locus_tag="VW528_000034"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9910935.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDTRDLIADLRQALEG"
     gene            complement(34880..36562)
                     /locus_tag="VW528_000035"
                     /pseudo
     CDS             complement(34880..36562)
                     /locus_tag="VW528_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(36734..38692)
                     /locus_tag="VW528_000036"
     CDS             complement(36734..38692)
                     /locus_tag="VW528_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9910936.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(38895..40517)
                     /locus_tag="VW528_000037"
     CDS             complement(38895..40517)
                     /locus_tag="VW528_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9910937.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            40623..42068
                     /locus_tag="VW528_000038"
     CDS             40623..42068
                     /locus_tag="VW528_000038"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9910938.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMEKSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGVDCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(42114..42308)
                     /locus_tag="VW528_000039"
     CDS             complement(42114..42308)
                     /locus_tag="VW528_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9910939.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(42409..43017)
                     /locus_tag="VW528_000040"
     CDS             complement(42409..43017)
                     /locus_tag="VW528_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU9910940.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDRKYELAIDFDKERLTFYLA"
     gene            complement(43042..43554)
                     /locus_tag="VW528_000041"
     CDS             complement(43042..43554)
                     /locus_tag="VW528_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9910941.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERDLHDSDF"
     gene            complement(43640..44575)
                     /locus_tag="VW528_000042"
     CDS             complement(43640..44575)
                     /locus_tag="VW528_000042"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9910942.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRIITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(44646..44945)
                     /locus_tag="VW528_000043"
     CDS             complement(44646..44945)
                     /locus_tag="VW528_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9910943.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(44935..45684)
                     /locus_tag="VW528_000044"
     CDS             complement(44935..45684)
                     /locus_tag="VW528_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9910944.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(45738..46097)
                     /locus_tag="VW528_000045"
     CDS             complement(45738..46097)
                     /locus_tag="VW528_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9910945.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEKKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(46099..47499)
                     /locus_tag="VW528_000046"
     CDS             complement(46099..47499)
                     /locus_tag="VW528_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9910946.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSIRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(47794..48033)
                     /locus_tag="VW528_000047"
     CDS             complement(47794..48033)
                     /locus_tag="VW528_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9910947.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(48065..48535)
                     /gene="ssbA"
                     /locus_tag="VW528_000048"
     CDS             complement(48065..48535)
                     /gene="ssbA"
                     /locus_tag="VW528_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9910948.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(48547..48837)
                     /locus_tag="VW528_000049"
     CDS             complement(48547..48837)
                     /locus_tag="VW528_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9910949.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(48990..50336)
                     /gene="asnS"
                     /locus_tag="VW528_000050"
     CDS             complement(48990..50336)
                     /gene="asnS"
                     /locus_tag="VW528_000050"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9910950.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVKNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(50352..50717)
                     /locus_tag="VW528_000051"
     CDS             complement(50352..50717)
                     /locus_tag="VW528_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9910951.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            complement(50710..51897)
                     /locus_tag="VW528_000052"
     CDS             complement(50710..51897)
                     /locus_tag="VW528_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9910952.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(51894..52382)
                     /locus_tag="VW528_000053"
     CDS             complement(51894..52382)
                     /locus_tag="VW528_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9910953.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGISQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            52703..53251
                     /locus_tag="VW528_000054"
     CDS             52703..53251
                     /locus_tag="VW528_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9910954.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            complement(53528..54256)
                     /gene="yaaA"
                     /locus_tag="VW528_000055"
     CDS             complement(53528..54256)
                     /gene="yaaA"
                     /locus_tag="VW528_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9910955.1"
                     /translation="MKILIPTAKEMNTDLPSIEAISLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTTQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(54335..54499)
                     /locus_tag="VW528_000056"
     CDS             complement(54335..54499)
                     /locus_tag="VW528_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9910956.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            complement(54566..56161)
                     /locus_tag="VW528_000057"
     CDS             complement(54566..56161)
                     /locus_tag="VW528_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9910957.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            complement(56173..56583)
                     /locus_tag="VW528_000058"
     CDS             complement(56173..56583)
                     /locus_tag="VW528_000058"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9910958.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            complement(56699..57490)
                     /gene="yghU"
                     /locus_tag="VW528_000059"
                     /pseudo
     CDS             complement(56699..57490)
                     /gene="yghU"
                     /locus_tag="VW528_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
     gene            complement(57503..60199)
                     /locus_tag="VW528_000060"
     CDS             complement(57503..60199)
                     /locus_tag="VW528_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9910959.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            60503..61687
                     /gene="mntE"
                     /locus_tag="VW528_000061"
     CDS             60503..61687
                     /gene="mntE"
                     /locus_tag="VW528_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9910960.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            complement(61830..63701)
                     /locus_tag="VW528_000062"
     CDS             complement(61830..63701)
                     /locus_tag="VW528_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9910961.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLTTLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(63698..64882)
                     /locus_tag="VW528_000063"
     CDS             complement(63698..64882)
                     /locus_tag="VW528_000063"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9910962.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDLDLLLQAENLRQAQGKEVNPQVIKETYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            complement(64894..65661)
                     /locus_tag="VW528_000064"
     CDS             complement(64894..65661)
                     /locus_tag="VW528_000064"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9910963.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            complement(65938..66786)
                     /locus_tag="VW528_000065"
     CDS             complement(65938..66786)
                     /locus_tag="VW528_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9910964.1"
                     /translation="MKLAVFTDSSAYLSAETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASEFPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            complement(66788..67162)
                     /locus_tag="VW528_000066"
     CDS             complement(66788..67162)
                     /locus_tag="VW528_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9910965.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            complement(67236..68588)
                     /locus_tag="VW528_000067"
     CDS             complement(67236..68588)
                     /locus_tag="VW528_000067"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9910966.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            complement(68612..69391)
                     /locus_tag="VW528_000068"
     CDS             complement(68612..69391)
                     /locus_tag="VW528_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9910967.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            complement(69378..70235)
                     /locus_tag="VW528_000069"
     CDS             complement(69378..70235)
                     /locus_tag="VW528_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9910968.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            70371..71339
                     /locus_tag="VW528_000070"
     CDS             70371..71339
                     /locus_tag="VW528_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9910969.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELESVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFVPSQLLGDGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            complement(71591..72547)
                     /locus_tag="VW528_000071"
     CDS             complement(71591..72547)
                     /locus_tag="VW528_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9910970.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKQDIENGLRAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKNTWYYLDAKEG
                     AMVSNAFVQSADGTGWYYLKPDGTLADKPDFTVEPDGLITVK"
     gene            complement(72551..72883)
                     /locus_tag="VW528_000072"
     CDS             complement(72551..72883)
                     /locus_tag="VW528_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU9910971.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            complement(72887..73186)
                     /locus_tag="VW528_000073"
     CDS             complement(72887..73186)
                     /locus_tag="VW528_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9910972.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHAVGDIQKEMVRMNDRMEEIEKGE"
     gene            complement(73196..73546)
                     /locus_tag="VW528_000074"
     CDS             complement(73196..73546)
                     /locus_tag="VW528_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9910973.1"
                     /translation="MAETNQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQLKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            complement(73549..73752)
                     /locus_tag="VW528_000075"
     CDS             complement(73549..73752)
                     /locus_tag="VW528_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9910974.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITNNILSIQLDEQREGNQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(73733..75115)
                     /locus_tag="VW528_000076"
                     /pseudo
     CDS             complement(73733..75115)
                     /locus_tag="VW528_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
BASE COUNT        22078 a        17112 c        14233 g        23085 t
ORIGIN      
        1 ctgatcaatt tgtcctgttc ttgtttcatt ttactataga acgatttgaa ggcgtttatg
       61 atatttagct gtacgagagt cttttaaaag tgttttgatg gtttggattt cttctttagt
      121 tgatttcata ttactattgt ataatgcttt ttgatttgag tctagtatta tactcttcga
      181 aaatctcttc aaaccgcgtc agcttcgcct tgtcgtatag atgttactga cttcgtcagt
      241 tctatctaca acctaaaaac agtgttttga gcagtctgca gctaactttc tataacaaaa
      301 tgaaataaga acaagacaaa tcaatcaaga caatcaaatc gatttctaag aatgttttag
      361 aagtagatgt gtactattct agtttcaata tattatagtt tgctctttga ttttcattta
      421 gtattagttc aatatctaaa aaaagaacct tgccaagcaa gattctttta gtgtctgact
      481 taaataattg ttcttctggg aactaaatcg aattaagctt cgatttctgt aaccatacct
      541 gaaccaacag tacgtccacc ctcacggata gagaatgtag taccttgttc tacggcgatt
      601 gggtgaatca actcaacatc gattgtcacg ttatcaccag gcattaccat ttcagtacct
      661 gctggaagtt cgattgaacc tgtaacgtca gtagtacgga agtagaattg tggacggtag
      721 ttgttgaaga atggagtgtg acgtccacct tcttctttag taaggatgta gacttcacct
      781 ttgaatttag tgtgtgggtt gattgaacct ggtttagcga taacttgtcc acgttcgatt
      841 tcatcacgtt gaacaccacg aagaaggaca cctacgttat ctccagcaag accttcgtca
      901 agttgtttac ggaacatttc aacaccagta acaactgctt tttgagtttc ttctttgata
      961 ccaacgattt cgatttcgtc gttgacttta acgataccac ggtcgatacg tcctgaagca
     1021 actgtaccac gtccagtgat tgagaatacg tcctcgactg gaagaagcaa tggtttgtca
     1081 gtgtcacgtt ctggttctgg gatatactca tcaactgtgt tcatcaattc cataacgatg
     1141 tcttcgtatt tagagtcacc ttcaagagct ttaagtgctg aaccttggat aactggaaga
     1201 tcgtcacctg ggaagtcgta ttctgacaat aggtcacgga tttccatttc aaccaattca
     1261 agcaattctt cgtcgtcaac caagtcaact ttgttcatga agacgataag gtgtttaaca
     1321 ccaacctgac gtgaaagaag gatgtgctca cgagtttgtg gcattggtcc gtcagttgaa
     1381 gctactacaa ggatagctcc gtccatttga gcagcaccag tgatcatgtt tttaacgtag
     1441 tccgcgtgtc ctggagcgtc gatgtgagcg tagtgacgtt tttcagtttc gtactcaacg
     1501 tgcgcagtgt tgatagtgat accgcgttcg cgttcttctg gagcagcatc gatagacgca
     1561 tagtctttag gttggttaac tgatgaaggc aagcgacgtg ccaaaacagt tgtgatagct
     1621 gcagttaggg tagttttacc gtggtcaacg tgtccgatag taccaatgtt aacgtgtggt
     1681 ttactacgat cgtatttttc ttttgccatt tgagtaaaag cctccaataa aatatatttt
     1741 atagatagac agtaggcaat acagtctaac tttccttact attttatcaa atttcagcta
     1801 aattgcaagt gttttacaac gtttttgtga attattcttg gtaaaaattt aactaactaa
     1861 tagaaagtac taaatagact tatccttcaa ggagttttca gcattatcca tccactcact
     1921 tgctgaaaat gtaaacagtc tctggtttat atagtaaaat gaaacaagaa tagggcaaat
     1981 cgatcaggac agtcaaatcg atttctaaca atgttttaga agttaaggtg tactattcta
     2041 gtttcaatct actatacaac ctcaaaactg tgttttgaac aacctgcggc tagcttctta
     2101 gtttgccctt tgattttcat tgagtataaa attgcagata aaacgataac aatcatagga
     2161 ctcagcagag tcaatgtagg tcgaagaata gcccccctaa tcgcttattt tatagcaaaa
     2221 tgaaatagaa actgaacatt ttgttgagga aggtaaatca attcctcaca atgtttgaga
     2281 aagaagggag gactattcca acttcaaccc actataaagc agacttacaa gatagggaac
     2341 ccatctccga taatatatag tagaatgaaa actgcacaat ttgattggag aattcaaacc
     2401 aatttctaac aatgttttag aaacaagggt ctattatttc aactgcaacc tactatagcc
     2461 cattgaagct ggaatagccc actgagactt ctaaaccact gctggagagg agtttgttta
     2521 gagtttcttt catttcccta aaaaatcaaa aagagagaac cagtctgatc ctctcttact
     2581 tatagatggt gctcactcaa atgttaaagg agctatttct tagagataaa ggaatttgaa
     2641 tacagctact gccgcaattg ctgctgcgat aggtgctact actggtaccc aagaatacca
     2701 ccattttgaa tcgcctttat gctcaccaag aactgatttt ggaaggaaag catgaaggag
     2761 acgtggtccc aagtcacggg ctgggttcaa ggcaggtcct gtaggtcctc caagtgatgt
     2821 taccaaagcc ataacgagga atccaagtgc caagtgagcc acagaaagtc ctgaagcagt
     2881 gtgtggagcc acctgtgctt taatagccaa gtcagaaaaa tcaactgttt gtcctgcttc
     2941 tattgccttt tgtttcatga attgaagcac ttcagcacca aagaagtttt tagtcaaacc
     3001 aagagctgca aagaacaaaa caaatgaacc aacaaactca ttaatcaaac cattgacagt
     3061 tgctgcatag cgactttctt ttgtaccatg gtcaatactt gaaatagttg agaaagttcc
     3121 caagatgtta tttgggtttt cagttttcaa gtagaatgga cggtatgttg ccacaactaa
     3181 ggcttggcca aagatagccc ccaagacttg cgcgataatg taaggtacca cttgtgccca
     3241 agggaaaaga ccgctaactg caagccctag agtgaaagca gggttgatgt gattcccaga
     3301 tacgttacca aacatcaagg ctgggatcat aacccccata ccataaccaa cagcgatgac
     3361 gatccagcca ctttggtgac ctttcgtacc tttaagttca acgttggcaa ctgcaccatt
     3421 cccaagaatg atcaaaatgg cagttcccaa aaattcagtg gcatacttca gtgcccatgt
     3481 gaaatccatt tgctagattc tccttaaaat tttttagata ttccttattt tatcaatttt
     3541 taccctattt agcaactgat ttctggaaat atttttaaaa aaataggatt catagtttcc
     3601 cagttcaaca gacttgtaag cgtttttatt ctttacgaaa gacctcgttc tcaaaaccag
     3661 gaaaaaagcc cacatctgtg agcttttcct ggttcctaga ttctaaaatc tattattttt
     3721 catcttcttt tttcttgaaa aatcctaatc ctagaccagc taacaaggtc atgataccag
     3781 cactagccaa ggtagcatta gcttctgtac ctgtgtttgg caattcctct ctcttacctg
     3841 tctcataagt cggaacttct gggtctggat tcactggagt ttcagttttt ggagtacctg
     3901 gttctggagt tggtttatct ggtgttggag tctcttgatt tggtagttct gggtttggtg
     3961 tcggaacact tggatgctct ggatttggtt gtgggattgg tttttctggt gttgataaac
     4021 ggtcatacct taccgttatt tctttatcac tagagtctga cgtaacttct tgtgattcaa
     4081 ctgttggaat atctggatct ttgtacttgt caatcttacc agatataacc tcgtcccagt
     4141 ttcctgttgt ccattcaccg taggttacaa ctcccgtgac cttgttctca gtttttgtac
     4201 ggcttaaggt tacaggttga acaacatctt cttttacatt ttggttcgta actttatcaa
     4261 cgtaatgaat gatacgcgtt atagtcttcg tctcagtaga ggttgctgtt ttgggaacca
     4321 ctgtttcctc aacattctca cggtagtaat agtcaactgt tgcaccgtct tctggtacgc
     4381 atttgcagga gttgctacca aggtgtatgt tgttttcctt gtgataactc ggtcttcttt
     4441 gtcctcagtt gttgttttcc cttcaatagt ttttgattct gtggtatact cagaacctat
     4501 cgctaaatca gcttttataa cagactctac caacttctct tgcctacctt ctttatagta
     4561 attggctgtt actgtagcag tggttggcgc ttcgctttct ctataaacta aggtcactgt
     4621 tctaccttcg cttacaatat tcccagttaa acttgcagaa tttgtatctg cttctttaaa
     4681 agtataatat tttccgtcag tagtagtcat gctactgagt tttttatctg tgacattgta
     4741 gctggtacca atcagttgtt ttttattggt aatgtaggtt ccgtcacttt ctttttctcc
     4801 aattccagta tcatttttca atgatagcaa acgcccttgt tcatcaacat agcgaacttt
     4861 cacattttct gagattagtt ctgccaattc tgaggttttt ttctttttct tgatttcttc
     4921 ggttattttc cctttctctt cttcgggaat atttagtttt ggaatgattt tttcaacaac
     4981 ggttcgtgat ggttccacag tatcttggat gactgaaaag tcagctagaa ttgggagatt
     5041 atactgaaca cggtgacttt gagtgtttac tcctactctt tcattattct ctgaaaatac
     5101 ttgtacggta taagaaacaa catcttttcc taatagaaca tccccagtag agaaatagcc
     5161 gccttttcct agtttgctat ctccagagcc cacttctttc ctaatcttat cagatagttt
     5221 tttaccagtc agtacattcg ttcgcacaat ccctttgtct acccctacaa agtgggagaa
     5281 ctttttgaac tcttcagaac cagatctagc ccaaccatta ttaagggcat ttgcttttgt
     5341 atttgtattc tctctcaaaa gtttggcgat tagaattata ttcatcggca cttagagttg
     5401 ctgctatatc tgactcttga ataccaactt ccttactacc atttctagcg gcagtatatg
     5461 tgtatttatc tgtttctact tctagctgat tatccttaac atgataggta attttctcct
     5521 ggtataagag tcttcagtag tttatatgct ctgtcatcat ctttagcttt agaagcagct
     5581 gagtttagcc aagttggggt attaggagta tctttatcag cctgtttcaa gacattattc
     5641 aagtcatttg ctagcctatt ggttgtcctc actgtgaaag ttttcttcca gtctgaacca
     5701 cttttattag acttagatat gctcatgaaa tcctggtcaa cattcaacac aatatcgcct
     5761 tgttttttag catcttccgt tgcttctgct tcaaattgat catggagttt ttgttgctct
     5821 ggtgtatagt ttttttattt acacgattta atgtactatt ttttatttta gtattaagaa
     5881 ctccattaat acgattttta aactctgctt caaaaaatcc cttaatttgt tggtgcgcaa
     5941 gttcaacatt caaacctttt ttatacaagt cttcattctg aacgatttcg gctacacggt
     6001 gaatggtttg attgtatacc gtcttctctt aaccagcagt atccttagtt ttaatgacta
     6061 ctcgataggt tcctttagaa ccgttatagt catctagttt taaaaaagca ttccaaacat
     6121 tcaaaaatag ttggaacttc ccttaataaa tccgtcactt tctttttcaa gatagcccaa
     6181 gcttgctcaa taggattcaa atccggtgaa taaggtggta gaggtaagaa aaagtgttta
     6241 tcttggatgc agagttcatc caaaatgttc ttgggatgaa aaccagcatt gcccatgaca
     6301 ataacatgag gtgtcttcaa agcaggtagg agttgcgttt tgaaccactc cacaaagaaa
     6361 tcgcttgtca tgcttttctt gtaaatcatg ggagctataa actctccgtc tacttgtcct
     6421 gcaacaattg aaattcgctc aaaacgacgt ccgctaatct tttcatagac tttctcccct
     6481 ctaggagccc ctgcataagg acgatagaga tagcggtcga ttcctgtttc atcaatataa
     6541 acaggtgcta ggtgctttaa actattaaaa ttcttaagaa ataaggctac ttttctgggt
     6601 cttgttcctt aaagctcgtc atcttttttt aaagtgacct taatctgctt taaagctgcc
     6661 catactgaag gaacagcaca atcaaaatgt gccgcaattt cccgtaaaaa agcatctgga
     6721 tgagcctcta caaaggcttt caattcctct aaaggaatct ttcggttttt gacgactcgc
     6781 tttttcctct ctatgtgttc ttgttcacgt ctttcttttc ccacgtgaag agagttctga
     6841 cgccaacacc aaaaaacttg gctgcctcga catggctgtg cccctctttg atgtaatcta
     6901 atgctcgctg tttaaaatca gtactatatg ccatagcttt attataatat gcttgtttaa
     6961 aactaagtaa ctatatcaat gttttgttat gagaagactt acttttttta ctgaaattga
     7021 atttttccta gcctaaacat catataaccc caaaattcaa tctgacctgg tttgaagaga
     7081 ttttcgaagc agataaggtt ttactaaaac taaaaaaaat gatataatca aactgattaa
     7141 atgttttaaa aataaaggag aatttgaatg tcttaccaag aaaattacca gaaatgggtt
     7201 gattttgtgg agcttcctga ctaccttcgt caagatttgg aaaatatgga cgaaaaaact
     7261 aaggaagatg ccttctatac aaatcttgaa tttggtactg caggtatgcg tggcttggtt
     7321 ggtgctggta caaaccgtat caacatctac gttgttcgcc aagctactga aggattggct
     7381 cgtttgattg agtcaaaagg tggaaacgag aaagaacgcg gtgtagcaat tgcctacgat
     7441 agccgtcact tctcacctga gtttgccttt gaatctgcgg cagttcttgc taaacacggc
     7501 atcaaatctt acgtatttga aagccttcgt ccaactccag aactatcatt tgcagttcgt
     7561 catctcaact gtttcgcagg tatcatggtc acagccagcc acaaccctgc tccatttaac
     7621 ggttacaagg tttacggtga agacggtgga caaatgcctc cacacgatgc ggacgctttg
     7681 actacttata tccgtgcaat cgaaaaccca tttgcagttg aagttgctga tgtggaaact
     7741 gaaaaagctt ctggcttgat tgaagttatc ggcgaagctg ttgacataga ataccttaaa
     7801 gaggttaagg acataaacat caacccagcc ttgattgaag aatttggtaa agacatgaag
     7861 attgtctaca caccacttca tggtactggt gaaatgttgg ctcgtcgtgc tcttgcccaa
     7921 gcaggatttg actctgttca agttgttgaa gcgcaagcaa ctgctgaccc agacttctca
     7981 actgtaacat ctccaaaccc agaaagccaa gcagcctttg cccttgctga agaacttggt
     8041 cgtcaagttg gtgcagatgt tcttgtcgca actgacccag acgctgaccg tgttggtgtt
     8101 gaagttcttc aaaaagatgg cagctacctc aacctttcag gtaaccaaat cggtgctatc
     8161 atggctaaat acatcttgga agctcacaaa aacgctggaa ctcttcctga aaatgccgct
     8221 ctctgcaaat ctatcgtttc aactgacttg gtaacgaaga ttgctgaaag ctacggcgca
     8281 actatgttca acgtcttgac aggtttcaaa tttatcgctg aaaaaatcca agaattcgaa
     8341 gaaaaacaca accacactta catgatggga tttgaagaaa gcttcggtta cttgattaaa
     8401 ccatttgtac gtgataaaga tgccatccaa gccgttcttg tagttgctga acttgctgcc
     8461 tactaccgtt ctcgtggttt gacacttgct gacggtatcg aagaaatcta taaagaatat
     8521 ggctactacg cagaaaaaac aatctctgtt actctttcag gtgtcgatgg tgctgaacaa
     8581 atcaaagcga ttatggctaa attccgcaac aatgctccaa cagaatggaa cgcaacagct
     8641 atcactgtcg tagaagactt caaggcacaa actgctactg ttgctgacgg tactgttaca
     8701 aacttgacaa ctcctccaag tgatgtgttg aaatacacac ttgctgacgg ttcatggatt
     8761 gccgttcgcc cttcaggtac agaaccaaaa atcaagttct acattgcagt tgtaggggaa
     8821 accaacgaag aatcacaagc taagattact aacatcgaag cagaaatcaa tgcatttgta
     8881 aaataaagac ctataaaaga tgagaccaac caacctcatc ttttttcgta tcaaatctaa
     8941 gcaattttag aaactttatg gcatactaga cttatcaatg aaagcgaggt aatctcatgg
     9001 aacaattttt agataacatc aaagaccttg aagtcactac agttgtgcgt gcgcaagaag
     9061 ctcttgataa aaaagaaact gcaaccttct ttatcggtcg caaaacttgc ccttactgcc
     9121 gtaaatttgc aggtacattg tcaggtgtcg tagctgaaac caaagctcac atttacttca
     9181 tcaatagtga agaaccaagc caactcaatg atttgcaagc attccgctca cgttatggaa
     9241 tcccaactgt accaggtttt gttcatatta cagatggaca aatcaacgtc cgttgcgact
     9301 cttcaatgtc agcacaagaa atcaaagatt tcgtaggatt gtaaaaaatc agctaacaag
     9361 tcttaaaatc acaaaaacgc ataatgtaag gtgcaaaaac cttgatatta tgcgttttat
     9421 tgttcctaaa acagttacat cagtatttca ttacagaaca ttttaatttt ggaagtcaaa
     9481 tcaattttta acaatgtttt agaaatcaca gtgggctatt ccatattcaa ttatccagat
     9541 tcaatcgcta ttatcgtttt cctagattac aaaaaaaact acgttttgct aatcaaaacg
     9601 tagtctcagc cactatttta tcttactgtc cttcttttac ttctttggtt gctacatctt
     9661 ctccaaacca tttttggctg atttcttgga acttgccgtc cttgtaaaga ctagaaaaag
     9721 cttcatttat cttcttaacc aaggttgtat cttccttacg ggctccaacc gcaaaagctt
     9781 ctgtttctag tccaactgta aagacattat aatcgtttaa aacaccttct gcttctaaat
     9841 aatagtttgc atagacacgg tcaatcaata gaccatcaat tcgatcgttt ttcaaatcaa
     9901 tcaaggcttc attaaaggtt tggtattgat tcgcttcctt attagcgaca atattcttca
     9961 aaagttctgg atttgcttca aagtccgcat aaccagatga accagcttga gctcctaatg
    10021 tctttccagt catatccttt gcagtcgtga taccagatga tttcttcgta accaatacct
    10081 gctcattctt catatatgag ttactgaaag ccaccttttc acggcgttcg tctgtagcgg
    10141 aatagccatt ccaaatcaga tcaatcgttc cttttgtcaa ttcagcttct ttcaaatccc
    10201 aatcaatcgg ttgccaattt accgtgattc cgtatttttc aaaaacagct gtagctaaat
    10261 caatatcaaa tcctgcataa gaaccatctt tctgagcaaa tcccattgga acaaaagtac
    10321 tatcaaatcc aatagtaata gacttgttag actggtactt tgaccaatta tctccactag
    10381 tttcgctaga atttttccca caagctacta agaacaaagc agtcatcaga ctgactaata
    10441 caagcatcca ttttttcatc catttttcct cctatttagg ttctactttc aataatacat
    10501 ctgcgatatt ttcagcaaac tgcaaatcat gggtaaccac aatctgggtc atcccaagtt
    10561 ccctattttg caagattagc ttctccactt ccaaacgtaa ttctggatcc agggcagaag
    10621 ttggttcatc gtagccaatg atttctgggt caatcatcat agcacgcgcc aaagccactc
    10681 gctgcttttg cccaccagat agtgagaaag gataggcctc tgcgtgtcct cctagtccta
    10741 actgttccaa gagtccactc gccttcttct cagcctcttc ctgcttcatt cccatggtct
    10801 tcacaggcga taaagtcaaa ttttccagaa ctgatagatg aggaaatagt tgaaaatctt
    10861 ggaagacaaa tcccagtagg ttgcgcttct gcaattcatc cagctctaaa ggttgtccat
    10921 tataaaagat ttgccctgaa tcaatggttt caagacctgc aagcatacgt aaaagagttg
    10981 tcttacctcc accagaaggt ccaacgatag ccaggatttg cttttcagga atacttagac
    11041 tgaaattaga caggatttgt ttgtctccaa agactttatt gatatttcgt aattctaaca
    11101 tggcagcctc ctatctataa taactatact tcttctcaac ttttttggaa ataattgtca
    11161 caatcccaat caaaatcaaa taaatggctc ctgccaagaa cataggaacc agactagcat
    11221 cgcggttagc agctgttcga ctagccaaga taaggtctga aattccgaga gcatagacca
    11281 aagaagtatc cttgaccaaa ctcataactt cattaaagac actaggaaga acgatcttgg
    11341 tcacttgggg caagataata tagcgcactc tgtcaaaagg gctaaacttc aagaccttgg
    11401 cagcctcata ctgtcctctt ggaatagtgt caattccccc acggaaaatt tctgcaaagt
    11461 aagccgcata attcaaaaca aaggcaataa tagctgcagg aaggcggtct aaacgaatcc
    11521 caatacttgg gagcacataa tagataaaaa tcagttgcaa gagtaagggg gttcctcgca
    11581 taacccagat gtaaatgtta atcagataat ggaggggctt ccaatggact tgcaaggcaa
    11641 aggcaatcaa aacgcccaag ggaatcgaaa atagcaagac cagtgcaaag acctgtacag
    11701 tcgtgctcgc accattcagt aaactcggta atatctcaaa taaataagac atactgcacc
    11761 tcctaaaagt aattgttcct attatagcat gaataaacaa gattaccaag tatttttgta
    11821 aaaagattct accatataca aataaaaacc gtagctatca aaagagataa actacagttc
    11881 tatatgttta aacattctta caatctctca aacagttctt gcatttgcac atcatctgga
    11941 accagtttta agtaagtgtg agcatggact tttgcttctt caaaatgtcc caattcacgc
    12001 aagagataga tatagtgttc cagaaattct ggattgtcct tcaaatctcc tgtcaactct
    12061 tgataatgct cataagcagt atccaaatcg tccatttctt gataagaacg agcaatcatc
    12121 cacttggtca aaagattttc tggctcctca ctctgcaagt ctagaatatc ctcataacgc
    12181 tcctgctcca gataaatagt ggctaaacga agcaagattt cttctgtatc ctcagcgtct
    12241 tcttttgcag taaggagata attttctgca ccactagcat catgcaattc ataagaaaat
    12301 tgtgaagcag ctagcaagag gcgagtttca aagggatttt tctctaatcc ttgcttagcg
    12361 atacgcaggg cttcttgaac ttgatgttcc ttatgtaaag cctgactgta cccatactca
    12421 tagccttcaa agtcaggaga aatggtatca agctgcttaa agtagagggt ggctttttga
    12481 tattcttctt gatcaaaata aagactggcc aactcaaaag ctgttaagtc atcgtattct
    12541 aactccaggg ctttttctaa aaactcagta gccgtttcaa atttccctaa ctgagcatag
    12601 gcaaagccaa ttcgttgata ggtggaaatg cccgtttgct cataaatcga gcgattatct
    12661 aactgggcat aggcttgaat agccgcttgg taattttcca actcactatc caactctgcc
    12721 aaacccaata tcaagagaga atcctctgag taggtcaagg cctccaataa tttctcacgt
    12781 gccacatctg tcaaaccttc cagctggtaa aggtctgcct tcagagccaa agacgagaca
    12841 taccagtcac tgtcagcttg gatttcctca agataggtaa aggcttcttc tatttgacca
    12901 tcctcgctag caattgcagc tagattaaga tgaacctctg gaaaatcctc tacaattttc
    12961 aggtaaattt ccttggcctg aggatagaaa ccaatccctt caagataagt tgccaactca
    13021 tacagaagat cacttgaatc attttctaaa gctttggcga aataatgctc agccttagtt
    13081 aaatcttgct cctccaaagc ctgtaacatc tgttgactat tgttcactct tgacttcctc
    13141 accctctaat tcatatagac cactgactac cttataccat tcgaaaatgg ctgaaatgac
    13201 aaccttagca gaggcataaa ccggaatacc aagtaaaact ccccagatac caaacataga
    13261 tcctgaagtt aacaaaacaa agagaacatt aataggatgg atgtttaatt gacttcccaa
    13321 aatcaatgga gagacaaaac ggccttcaat agtttgttct acaataaaga caatcactac
    13381 tttcaaaagc atgactggac cagcaatcaa acccaatact agggcaggaa gcatggctag
    13441 aaagctacca agataaggga ccagatttaa aataccagca gtaaccccca gcgtaaccgc
    13501 atagcgtaga ccaataatct tgaagaagat gataaacatt actgctacaa taatagccac
    13561 tgtcacttgc cctcgaacat agttggacaa ctgttgattc acatctgata gaacttgtcc
    13621 aacaggttcc ttcaattttc ttggaatgaa ttgggtcaaa tagttacgca agcctttccc
    13681 atcacgcaag agataaaaga gcatgaaagg aacgataatc aaggcaacaa tcacttgaga
    13741 agctccgcta ataaaggcac tcacccagtt gactgcctga gatgaaacct tacttgccaa
    13801 aactgtagcc tggctagaaa aattggtcaa aacttgctct aattgaggtc tgaaatcatc
    13861 tggcaggtgc tgggctacca atccattaac aatcctatct atatcttcta agtaaacagg
    13921 aacgtttctt gcaaaggtca aaacctgacg ttgcagattt ggaatggcga ctgccaagcc
    13981 ccaaatgata aagagagcga tgataacaaa gacaatagtg atagctataa cacgattaac
    14041 cttatgcttc tccatccaat caacaatagg attcaacaaa taatataaca aaccagacaa
    14101 aatgactggc aacatcacaa ctgctataaa gtctaaaaca ggtgaaaata gaaaactaat
    14161 cttacttaaa ataaaaagat tcaatcccaa taataaggtt actaaaaata ccgtaattgc
    14221 cttgttatct aaaaaccact tgaaaaacca agataggcta aaatgtttct ctttttgctc
    14281 cataaatact tcctttctat tgttctctca ttataccatt tttgaaacaa aataactctt
    14341 ttttaaagtg cttacatcta attgaatgtc tttaatttgc ttgtttttgt gatataatag
    14401 tcttatcttt acacagaggt atagtaatga aagaaactgt ttattttgga acttatacac
    14461 gtcgtacttc tcaagggatt tacaaggcag actttgatac agaaactggt cagctttcaa
    14521 atctagaact ttttgcagct gagccaagtc caacctacct tgcctttgac cagcaccaac
    14581 atttatacac tgttggtagc caagacgata aggggggaat tgcagcctat caaactgacg
    14641 ggactgtgtt aaatcatgtt gttgaagaag gagctcccca ctgttatgtt gctgtcgatg
    14701 aaaagcgtga tttggtttac gcagccaact atcacaaggg acaagtcctt gtttataaac
    14761 gccaggaaga tggtagtctt ctacttagtg atatggatca acacagtggc caaggtccac
    14821 atgaaaatca agcttccccc catgttcact atacagattt aacacctgac cactatctag
    14881 tgacctgcga cttgggaact gaccaagtca tcacctatga cctcgatcaa gaaggaaaat
    14941 tatctaagct ctatacctat cacagcaagc caggagcagg ctcacgccat atcattttcc
    15001 ataaccacta taaaatcgct tatctcattt gtgaactcaa tagtactatc gaagttttaa
    15061 tctacgatgg cgttggcgaa tttgaacgta tgcaggttat ttcaacttta ccagaagctt
    15121 acgaaggctt taatggaacc gctgctattc atctctctaa agacggtaaa tacctctacg
    15181 cttctaaccg tggccatgat tctatcgcag tatataccat ccttgcggac ggtagcttag
    15241 agttattaga aatcgttcca acgcatggtc agactccacg tgattttgat ttgacacccg
    15301 accaaaaatt tctcattgtt gtccatcaag actctgacaa tgcaactgtc tttaaacgta
    15361 attgtgacaa tggtcgtcta gcagaactct ccaacgactt ccatgttccc gaagcagtct
    15421 gcatccgttt tgctccttaa aacaccataa aaaatgaact tggtattttc aagttcattt
    15481 tttcttatag tctatttccg acattaatac ggttaatagc acgttgcaaa gcaatcttag
    15541 cacgacgttc ttggtcaatc aaatgtttgt cttgtgcttc ttcaattgca cgttctgcac
    15601 gaagtttggc acgttctgca cgactgatat cgatatcacg agcacgttct gcagagtcag
    15661 cgacgattgt gatcatatca ttggcaattt caataacgcc accgtttact gcaatccagt
    15721 tcacgtgatc tttatcatcg atacgtttta cctttacttc atcaactgct aaaaccgcaa
    15781 tcatattttc atgtcgtggc aagatcccca tctcaccatc cagagttcga accgatacat
    15841 agctggcatg gtgatcatag acgagaccat ctggtgtcac gatctggaca gttaactgag
    15901 ccatagatca cctcttaaaa tcccattttt tcagcttttg caatcacatc ttcgatagaa
    15961 cctacaccac ggaaggcatc ttctggcaag tggtcgtatt taccatcaag gatttcctta
    16021 aagccacgta cagtttcagc aactggaaca taagaacctg gctgaccagt aaattgttcc
    16081 gcaacgttga agttttgtga caagaagaac tggatacgac gggcgcgagc aaccaaggtc
    16141 ttttcttcat cagaaagctc atccatacca aggatagcaa tgatatcttg caattcatgg
    16201 taacgttgaa ggacacgttt tacttcagca gcaactgcat agtgctcttc tccaacgatt
    16261 tcaggtgcca aggcacgtga gcttgaagca agtgggtcaa cggctgggta gatacccaat
    16321 tgtaccaact tacgttccaa gtttgttgtt gaatccaagt gagcgaaggc tgttgctggc
    16381 gctgggtcag tatagtcatc cgctggcaca tagatagcct ggatagaggt tacagaaccc
    16441 ttcttggttg atgtgatacg ttcttgcaat tgacccattt ccgtagcaag tgttggttgg
    16501 taaccaacgg ctgatggcat acgacccaaa agggcagata cttctgaacc agcctgagtg
    16561 aaacggaaga tattatcgat aaagagaagc acgtcttggc cttccacatc acggaagtat
    16621 tcagcgattg tcaaaccagt aagggcaaca cgcatacgtg ctcctggtgg ctcattcatc
    16681 tgaccaaaga ccatggctgt tttctcgata acgcctgatt ctttcatttc ccagtaaagg
    16741 tcattcccct cacgagtacg ttccccaaca ccagtaaata ctgaaatacc accgtgctct
    16801 tgggcaatgt tgtgaatcaa ttcttggatt aagacggttt taccaactcc ggcaccaccg
    16861 aaaagtccaa ctttaccacc tttaaggtaa ggggcaagaa ggtcaataac cttgatccct
    16921 gtttcaagga tttcagaaga ggtagacaac tcatcaaaag ttggagcttt tttatgaatt
    16981 ggctgacgct ctgcgtcttc tgtaaaagga gcttccaaat caatggtatc tcccaaaacg
    17041 ttgaagacac gtcccaaagt ttctttacct actggtacag agattggacg acctgtgtcc
    17101 aatacttcca ttccacgagt caacccatct gttgattcca tggcgatagt acgaaccata
    17161 ccatctccta actccaaggc tacttcaagg acgatttttg tttttctttc gtcatttttg
    17221 tagacgacaa gtgcattgtt aatctcagga agtttttccc ctgctgcaaa caaaacgtct
    17281 acaacgggac cgataacctg agcaatttta cctgaactca tctccttctc ctattctata
    17341 taagatactg tcggttccta gctcaactag gtcctaagtt cattttcata cgagctggac
    17401 tagagcctat tctaaggcac tagcacctgc tacgatttct gtaatttctt gtgtaatcgc
    17461 cgcctgtctg gcacggttat actgaattgt caaatcattg atgactttct tagcattatc
    17521 tgtcgctgtt tgcatggctg tcatacccgc agcattctca gctgtcttgg catcgataat
    17581 ggcaccgtaa atcatacttt ctgcaaactg aggcaacaac tgctccagaa tttcttctcg
    17641 gctggtttcc aattcaaaag tcaagctgta ctcttcatcc gcttcatttg gatccaagtc
    17701 aacaatcgga agcatttgtt ccacacgcat ttgactggtt agcgtattga catggtggtt
    17761 gtagcaaaca taaagctcat caaagagttc attttggtac atttcaacag tttttgaaat
    17821 aatcttacga acttgatcaa agctaggttg gtctgccaag ccacgtaatt cataaagtgg
    17881 ttgaataccg cgagccttaa agaaatcagc tcccatccca ccgatacaga tcatttcaaa
    17941 acctttaccg tctgggtggt attcttcttt caactccata acagctttca aaatagagga
    18001 attataacct ccaaccaaac cgcggtctga agtgataacg atatagcctg tcttcttcac
    18061 agaacggcta atcaacatcg gattagttga agcaccagct ccattaccat gaaggatatc
    18121 tgtcaaaagt ttacgcactt tctgagcgta aacttggaag ttgcgagcag cttcttcaga
    18181 gcgacctaac ttagcagccg ataccatttg catggcatta gtgatttgac tcgtattttt
    18241 tgttgaggcg atttttgttt taatatcatt tagagacact gccatctgac acctctattc
    18301 ttattggaag ctagattgat tgagaaactc tgtaatcgca gcatccaaga ctgcttcttc
    18361 tggcaagtct tttgtatcac gaatggtttc caaaatctct ggatgttgag catcaaagaa
    18421 ggcatggaac tcttcctcga aacgaacaat atcatctact ggaacagtat ccaagaaacc
    18481 atgtgtcaaa gcataaagaa tggttacttg tttctcaaca ggtaatggtt tgtgaacagg
    18541 ttgtttcaag acctcaacgg tacgacgtcc acggttcaac ttagcctgtg ttgctgcgtc
    18601 caagtcagaa ccaaacttag taaaggcttc caactcacgg tatgaagcaa ggtcgatacg
    18661 aagtgtacca gcaaccttct tcatggcttt gatttgtgca gaaccaccta cacgagatac
    18721 agatgaaccc gcatcgatgg ctggacgaat acctgcattg aagaggccat cgccaaggaa
    18781 gatttgtcca tcagtgatag aaatcacgtt ggttgcgata taggctgaga tatctcctgc
    18841 ttgtgtctcg ataaatggta gggctgtaat tgatccacca ccaagttcat cagaaacttt
    18901 agctgagcgc tcaagcaaac ggctgtggag atagaaaaca tcccctggga aggcttcacg
    18961 acctggagga cgacgaagca agagcgatag ttcacgataa gctaccgctt gttttgatag
    19021 atcatcgtat acaatcaaaa catgcttacc ttgatacata aattcttccg ccatagcaac
    19081 cccagtataa ggagctagga agagcaatgg agatggttgt gaagcagagg ctgtcacaac
    19141 gattgtgtag tccaaggcac cgtactgacg aagtgtttct acttgcgtac gaactgttga
    19201 ttctttttgt ccaatcgcga cgtagataca gatcatatct tgatcttttt ggttcaagat
    19261 tgtatcaatc gcaatggttg ttttccctgt ctgacggtca ccgataatca actcacgttg
    19321 accacgacca atcggtacaa gggcgtcaat agctttcaaa ccagtttgca atggttctga
    19381 aacagactta cgttgcataa caccaggagc tggtgcttct actggacgag ttttatcagt
    19441 gtggatttct ccaagaccgt caactggacg accaagcgga tccacaacac gaccaatcag
    19501 actttcacct acagggactt ccatgatttt ccctgtacgg cggattgtat cgccttcacg
    19561 gatatctgta aagtcaccta ggatgataat accaacgtct gttgactcca agttttgagc
    19621 cataccataa gagccgtttt caaaattcaa caactctcca ctcatgacat tttcaaggcc
    19681 atgagcacgc gcgataccgt ccccgatata ggttacaaca cctgtttcag tcacatcaaa
    19741 attgggtttg aaattttcaa tttgttgctt aattaaagcg ctgatttctt gtgcgttaat
    19801 tgccaaaaga acaccacttt ctatttcaaa ttttctttaa caactttaag ttgttgttta
    19861 atactcacat caattgtctt gtgattggca aaaatgacaa aaccaccaat gagactttca
    19921 tcgatttgtt cttttacact ccttactttc agagacattt ttttctcaat caaagggagc
    19981 aaacgagtct tctgttcatc agttagagga tgagcagacg taatcgtcac ttcaaatcga
    20041 tttgtttctt tttcaagtcg gttcaagcaa tctacaagca catcataaaa aagatttgct
    20101 ctgtgattgt aggccagaac ctggataaag ttttgtaata aaggcgacac agaatcttgg
    20161 aaaaaagcaa ttgttttttc cttatccgac tcgtctactg ccacttgttt taaaaaagaa
    20221 ggcagacctg ttttttcaac aacttgcttg atttgagtca agtctgaaaa gatacggtct
    20281 tcttctcctt tttcaagtac caattggaca aaaggcatgc tgtatttttc aattaccttt
    20341 actgttttct tgtccattaa gcttctccta gctgatcgat atactgatca atgagtgctt
    20401 tatgggcatg actgtcaagg ttttgtgaga tgattttacc agctaagctg atggtcaaat
    20461 ctgcgacctc acccttaaca ctctgtaaag cttctacttt attttgagca atttcttgat
    20521 tggctttttc ttttaagtgt cctgcttcta gtttagcatc tgctaagata ttagccttac
    20581 tttgctcagc tgtttccttt gcattttcaa tgattgtctt agcttcttta cggctaccag
    20641 ccaattcatc ttcgcgtttt tgagccaata cttctgcttt ttgacgggct tcttcagctc
    20701 tatcaatatc tgaagcaatt ttttcagctc tttcttcgaa aatgcctgta atattagacc
    20761 atgcaaattt tttaatcaag actagcaaaa gaataaaaga gccagtgatt aaaataaaat
    20821 taccaattaa ttcacctact gttacgtgca tcagttactc ctttctattc ctcttcaata
    20881 ttaatcttat tccctaaata cacagatgtc aaaagagtaa aaacataagc ttggatgcag
    20941 gaaataaaga cagaaaatgc agtccaagcc aaattagctc caaaggctac tggataccaa
    21001 taaatagctt ggtgggaaag aagaagtaac aaactcgtca tgacctctcc tgcaaagata
    21061 ttcccaaaaa tccgcaaagc caaagataag aagtttgtaa attcttccaa gatattcatc
    21121 ggtatgacaa aaacaggact cataaaagat tttatacttt ttttaaatcc acgacgacga
    21181 acgctttcta tatgtgtcaa caaaatgaca agaaaagata aggttaagtc atactgtaaa
    21241 ttagcggttg gcgaactcca ccagttagtc ccatcgatcg tttgaagctt tgtcattaag
    21301 ccaaggttat tggcaatcac catgaaaagg aataaacaaa ggaaaaagag tgagtaatct
    21361 ttcatgtagc gcgaaccaat gttaggttct gtaaatccaa taacaaagtc atagacatac
    21421 tcaagtatat tttgctttcc tttgggtttc aaggtcatat tgcggcttgc ccaataaata
    21481 aagacaaaaa taactcccac aatcaacaaa gtcatggcta acatagtcag attgaagata
    21541 acaggaccaa tagagatgat tggattaata ctttcttcca tctaaaatcc tcccttttct
    21601 tctcgttcca tgtatttatt tgataataaa tgagaagaca agagttacaa agaaagttcc
    21661 ttcaataaag gcaacaccta aaaacatcaa actacgaaac tcagaaagca tatctggttg
    21721 gcgtgctact gatttaaaca gtccattcat caataaacct tcaccgacag atacgcccat
    21781 acaggcaata cataagccta aaaatgttaa attcataaca aattctcctt taaaataaaa
    21841 tttacttact tagtgtagtc ctaaaattta aatttgtcaa cttttaaccg ctattggaag
    21901 cgtttaataa aaattatttc cattttgcta aatttcaagt tcaagttagc catccagaag
    21961 tcttctctgg gtgacttgta gtccaagcat tttttaggat agttgttaat ccacttttcg
    22021 atgaatgcga cttctttggg agtcattttc ttggttccct taggtaacta tctacgaatg
    22081 agcctgttgt gattctcatt agttcccctt tcccaagagg cataggggtg cgcataatag
    22141 atgtgctcct cagaaaatat atcaaacaag cgattgaatt ccgttccatt atctgccgtg
    22201 atggaaagaa tcttgtgttg ttttaagatg agttttagag cctgattgac cacctcagca
    22261 cttttatttg gaatcaatcg gatgatctga tgtctactct ttcgatccgt caagacaagc
    22321 aagcagtagt ttttcgctct cgtaagtaga accgtatcaa tctcataatg cccattctcc
    22381 aagcgaagat tgatagcttc aggccgctgt gcgatggatt gaccagcagg tttaaagttg
    22441 atgctagcct gtttcttaag cgcttttcct tttctagggt aaagcagatc ctgtttgctt
    22501 aaccccaatt ttccatgatg aatccaatag taaatggttg aaattcccac gttaacccct
    22561 ttagccatca ccatcatttc aggcgaaaat ttttggttat gatagtggag aatcttttcc
    22621 tttagttcct tggtcacgct taatttcctg accgagcgct tgcgattgtt ttcataagac
    22681 tgttgagcat agtcggcaga ataaacctct ttgaagcgcc cttttccaag acattgtcgg
    22741 actgtcccac gcttgatttc agtgtggata gtttgaggaa cttttccaag cagagaggca
    22801 atttctctat ttgattttcc ttcttttttc catcgttcga ttaagcgacg gctatcgatt
    22861 gtcaaatgtt taccttttgt agtataatgg ttttgcatct ctgtgccttt cttgtgtttg
    22921 tggttgaaca acaagtataa cacagaggtg ttttcttatg cctacaagag ctttcattat
    22981 ttccattttc ttttggattt cactctattc tgaaaaactt gtgtatattt actgaagcta
    23041 gcaaggctta cctgtaaatt taatgaaagc aacacaaaat ccgagagggg aatctcggat
    23101 taatgtcaat ttaaaaagat agagagtttt tagtttaaat aaattgtttt aaaatatcaa
    23161 caacatcact tcttttctta acctgataag ccttgattcc taattttggg gctacgatta
    23221 tattgtcctc aatatcgtct agaaagacac aatttctagg ttataactgg tatttatcga
    23281 tagttactca tatacatcag tccacctcca tactcgcctt acagattctt ttggtaataa
    23341 tatctttgcc taatgtagag atagtcttgc aaagaaaaaa cttccttgta gccatgtttc
    23401 tgataaaagt ccagtgcctg gaactggtaa gtattgacaa aggcaaaaca acaatttcga
    23461 ttcttagctt cactttctgc ctgttgcaat agttttgaac cgattccttg ccctcgcagt
    23521 tcctctttta caaacaaata ctcgatttct agccaatttc caaaagtctc tgctatcaaa
    23581 cccgcaagga gattgccctt ttcatcttcg acataaagat taagtggctc actttcagcc
    23641 tcttctcttt ttgaacggtt ataaacacga atcagattcc ctatttcttg cgatttatgt
    23701 gattccttat tttccaatct aaggtataat gaaatgaaat aaaacatgcg caaatcgatt
    23761 aaggaattta atctaatttc taacaatgtc ttagaaatca aagtgtacta ttttaacttc
    23821 aatgcattat acatctaata ctcaatgaaa atcaaagagc aaactaggaa actgacgaag
    23881 tcagtaacca tacatacggc aaggcgacgc tgacgtggtt tgaagagatt ttcgaagagt
    23941 agcaaaatgg aaaaaggagt gagtgaagca catcgcctcc ccactccttt ttctgttttt
    24001 aggctgtttt ttcaaccttc aagattttta catcatagct accaacaggc gtttcaatgg
    24061 ttgctgtatc acctgttttc ttgccaatca aggcctgccc aattgggctt tcatttgaaa
    24121 ccttacctgc aaaggcatcc gcaccagctg aacctacgat aatataaact tcttcttcgt
    24181 cctcaccaat ttcttggatg gtgactgttt taccaatcgc tacttcgtcc tgggcaactg
    24241 cgtcgctatt gacgatttca gcatagcgga tttttgtttc taagctagag atttgtcctt
    24301 cgacaaaggc ttgttcatcc ttagctgctt cgtactcact gttttctgaa aggtcaccgt
    24361 atgaacgggc aatcttaatg cgttctacca cttctggtcg acgaaccaat ttcaattctt
    24421 ctaattcttt ttcaagtttt tccttttcct caagggtcat aggatatgtt ttttctgcca
    24481 tttttctcaa ctttcttctg ataatatttt ctaaagaaaa ttatgtgaag tatcacataa
    24541 ttttagtttg tttagtttaa tttgctgttg acatgttcag cgacattgcg gtcgtggtct
    24601 tcttgattgt tagcatagta gaccttgcct tctgtgacat ctgctacaaa gtagaggtta
    24661 tcgctcttag tttgattgat gcttgactca atcgcatcca gacttggact atcgactgga
    24721 ccaggcatga gacctacatt tttataaaca ttataaggtg aatcaatgtt ggtatcaatc
    24781 gcaacatcct cagctagact gatattttgc cccagttttc cttgggcata caagattgca
    24841 atattacttt gaagtggcat atcacgattc aaacgattgt agaatacacc tgcaatgagc
    24901 ttacgatctt ctgtcttggc accttctttt tcgaccaagg aagcaatggt caacaactca
    24961 ttgacagtca aatttttaga tttgatagta ctatagtaag gagataggtt cttatccata
    25021 gcagctaaca tctcatcaat caagctctca atagttgtgc tttccttgat agagtatgta
    25081 gctgggaaaa ggtatccttc caaacgataa cgcgcaccgc tgtcttttac aggcaaactt
    25141 tccagtaaag taggatattt cgctactgct tgactgataa acgtctcatc ttgaacttta
    25201 gccaagaaag cctccgctgt caaagactct ttgaagtcac cttgcaattg acccacagct
    25261 tgagcaatct gatccaaggt ataaccttct ggaattgtca aagtcgcaag tacaggttct
    25321 tgcggttcat ctgttccacc tttttgcaac tctttgagta agtcttctgt actcatactc
    25381 ttttgcaaat tgtagtaacc tgctttcaag tcggtataat ttttatactt ggcataaaaa
    25441 ctaaaaatca gaccatgctt taccaaacca gctttttcaa gcgtcgtacc gatttcttga
    25501 acgtttgaac cttctggaat tccaaccgtc acatatttct tagaattagc atcgataggt
    25561 aacaaagaat ctagcacgta ctggtaacca aagtaaccac ctgctgagag caaggcaagg
    25621 aatacaagga atgaaatcac aaaagctttc acatgtgact tcttctcttt cttaggctta
    25681 acgggttttg ctccttctct acggctacga cgtgatgtat cgctgatgat atccactgtt
    25741 tctgtagcag gcgtaggtgt ttctgaacct gcttgttctg cttttttctc tgccgtcgta
    25801 taggaaacag ctacccttgt tggggtttca ttgtattctc tttcaagttt cttaggtcta
    25861 acaggacctg gacctggtct tgatccactt tcttccgctg gagaagaagg tacatcttga
    25921 cttggatgac ttggaacacc aggagtttct ctttgaatct catctgctgg agaagctggt
    25981 acaccttgac ttgggtgagt aggcacggta ggagcttttc tcataatctc ctctaccgtt
    26041 gacaaggaat cagccatgag ttcttcagct gaaggttcat ttgcaggagt gcgaactact
    26101 gcctcatctt ctttcagaac ttcatcatag ccttttactt tttctaaatc tctcagaatc
    26161 tgctctttaa agcttaattt ctcttcttct cttgactttt cactcaaaag tttttcctcc
    26221 ttgttgataa tccataatat tatatcttaa aagtccaaaa aaagcaatct atgatacttt
    26281 tcctaactta ttttttcatt tcccagacca tatcatacca tgtttcccct gcaaaggttg
    26341 actgggactg gccttcgttg acaaatccat gtttttcata ataggcgatg agatagtcat
    26401 gacaggttag attaatgcca tctctctcat cttcaagagc aacttctttc aaggctgtca
    26461 gcaatttctg acccagtccg agtccctgtg cctccttggc aatagacaga caggtcacag
    26521 agatataacc accaggctca tgactatagt cttttatttc ttctgtaaaa gactggtctt
    26581 gcagatggcg gtgcaggcca actggtcctt cgatataacc catgattctt ccttcttttt
    26641 cagcaaccag aaaagaggtc tgaatttctc tcaaatgtgc ttcaaagaca gaaggaggaa
    26701 tggcttcttc gaccgaaaaa ttttcaaatt caagttcaac aatccgatcc aaatcttcta
    26761 atcttgcttg tctgattttc attgttcctc cagataaaag ggattaaacc aaatcatact
    26821 atagccctgg ctagttgcat agagcaaagt tgcttcttca tcaacaaaac cgttcatttc
    26881 aaaataggaa agcagctcat caggactctc caaacgaatc cctttgtaat ccagctcaac
    26941 tgccacctct ttcaaggctg caagaagaag tgttcccaag ccctgtctct gatggtcaga
    27001 ctcgatgact aaagaatgta cttttagata ttgcggattg tctgactggg gacttgataa
    27061 aatatagcct aaaagttgat tttcatccct agctagaaga aaggtatccg cacacttacg
    27121 gatactttct tctaaaatat gggaaagttg ctgcttttca gctggaaaag acgaggtctg
    27181 aagtgcccct atctcaggca aatcagactt gcttgcctga atgatcttaa ttggaatttc
    27241 catgggaaca tcctattgaa cattgcttgt caagttagac aagagacgct caaatgagta
    27301 ttcataggtt tggatgtctc ctgctcccat aaagacgtaa acagcattgt catggtctag
    27361 gagcggagaa acattttcaa cagtaatcac ttggtgtttt ttgttgattt tgttggctag
    27421 atcttctacc ttaacgtcac catgatctac ttcacgagcc gagccataaa tttgcgctag
    27481 ataaacagca tctgcttggt ttaaagcatg ggcaaagtcg tccaacaagg caatggttct
    27541 tgtaaaggta tgcggttgaa agactgctac aatttccttg cttgggtatt tctgacgagc
    27601 cgcatccaag gtcgcaataa tttctgttgg atggtgggca aagtcatcga taatcactgt
    27661 atcattgaca attttctcag tgaaacgacg tttaacacca gcaaatgttt tcaagtgctc
    27721 acgcaccaag ttcaaatcaa atcctgctgt gtaaagaaga ccaataacgg ctgtcgcatt
    27781 catgatattg tgacgaccaa aggttggaat gtggaattgc cccaagtttt gtccacggaa
    27841 atgaacggtg aaggttgaac cagttattga acgaagaaga tcactagcta caaagtcatt
    27901 gccttcaact tcaaaaccat aataataaat tggtgcatca gacgtaatct tacgcaattc
    27961 agcatcttca ccatagacaa aaagaccctt ggtgatttgt ttggcatagt cgttaaaggc
    28021 attaaaaaca tcctcgagac ttgtgaaata atctggatgg tcaaagtcaa tgttggtgat
    28081 aatagagtat tctgggtggt aaggcatgaa gtgacgctca tattcgtcag attcaaagac
    28141 aaaatatttg gcattggccg aaccacgacc tgtcccatct ccaatcaaga agctggtatc
    28201 tgtaatgtga gacaagacat gagacaacat acctgtcgtt gaagtttttc catgtgctcc
    28261 tgctactccc atgctaacaa agtcacgcat aaagctacct agaaactcat ggtaacgttt
    28321 gtagctgata ccattttggt ccgcataggc aatttcgacg ttgttatctg gacgaaaggc
    28381 atttccagcg ataatttcca tatcaccgtc tagattcttt tcatcaaaag gaagaatggt
    28441 aattcctgcc tgctcaagac cgcgttgggt aaagtagtac ttttcaacat ctgatccctg
    28501 aaccttgtgc cccatctggt gcaacatcaa ggccaaggca ctcatccctg atcccttaat
    28561 tccgataaaa tgatatgtct ttgacatgtt ttctccccta ttctgtcatt ctggtcagat
    28621 tcaactcttg ggcaacccga cgttcttgtt ctgtttgttt acttttttta ttgtagattt
    28681 ggctcttctt tagaaaatca taattgtttt tctttggagc aggtgctgac acttcttcat
    28741 tcttggtagg gatagaatga acttcttccg ccaagatata atgagactgg gtcaattttt
    28801 ggctatattt gacaaattca ccaggatttt ccttttggaa aggagttgtc ggttgattgc
    28861 cctgtctaac tagactgggc tgagaatgac gtctcgcaag gctgaaatcc tgagttagat
    28921 agttagcaga gcgtttcttt ttcaagtctg cacgcgcttc ttcacgcgcc acctccgcat
    28981 agctctttcc ttctttttta acccctaaag gagctttttt aggtttccct acttgctttt
    29041 caatcggttg tactggtttt tcttcagcaa taggagccca ttctaaataa tttttatctc
    29101 gatactcacc cttgatatta ctgatcagat cagactcatc atagagattc atgactggca
    29161 tttcagtcaa catgacctcg tcatctgaca ccaatggaaa tcgttcttgt ttcattttct
    29221 atttcctttc aacacttcat tatagcgtat tgtcttgatt tttcaagtgc tggcttcaga
    29281 aattcccaaa atttctctaa tttctgctag ggtcagacta ccacgtgact ctgtgccgtc
    29341 caatacttgt gacaccagat gtttcttttg ttcttggagt tcctgaattt tttcttcaat
    29401 ggttcccttg gtcaccaagc gatagacctc aaccgtttct tcctgaccca tccgatgggc
    29461 acggccaatg gcttgcgctt ccaccgcagg attccaccaa aggtcaacca agatcactgt
    29521 atctgcacct gtcaggttca gaccgacccc accagccttg agggaaatca gaaaggcatc
    29581 tctttctcct tgattaaagg ccttggtcat gtcttgtctt tccttggctg gggttgaacc
    29641 cgtaatttta aaggaagtca ggcccaagtc tggcagttct tgttcaattt tttccaacat
    29701 tcccttgaac tgagagaaaa tcaagacacg gtgtccgccg tctgccacct gtaccagtag
    29761 gtctcggaga ctatctagtt tgccgctggc tccctgataa tcttccataa acagggcagg
    29821 agtgtcacat atttgacgca agcgcatcaa accagataaa atttccacac gacttcgctg
    29881 aaattcctgt tctgacactt gagccagatg gtctcgcatc tgttgtaact gggcaaggta
    29941 aatagccttt tgctggtctt ccagttcatt tttataaacc acctcaatca agtctggcaa
    30001 ttcagtcaga acttcttctt tcttgcgtcg catcacgaaa ggcttgataa actgagccac
    30061 tcgctctgct ggcaatttca taaattcttt cttgcttggc aaaagtccgg gcatgacgat
    30121 ttggaaaata gaccacaact cacccagatg gttttcaatc ggagttcctg acaaggcaaa
    30181 gaccgacggc accacaaatt gtctcaaggt ctgggcaatc ttggtctggg catttttcat
    30241 gacctgagcc tcatctaaga aaaggaagtc aaaggccatc ccctgataaa actcactgtc
    30301 ctgacggaag gtggcatagc tagtcacata gatttgatgg ctctcggcaa gaatctcctc
    30361 acgacttgct ttcaaaccat gaacaacaac cacatccaac tgtggggcaa atttctgaaa
    30421 ctcatctgcc cagttgtaaa tcaaacctga cggagcgaga atcaagactc gactttcttt
    30481 tgtcacttga ctagtcaaaa aagcaatggt ctgaagggtt ttcccaagtc ccatatcatc
    30541 agccaaaatc ccaccaaaac cataatgatg gagcatctgc aaccagccaa ttcccttttc
    30601 ctgataatct cgcaagtcag ccttgacctg agttgcttgc aaaggaaagt cctctggatg
    30661 cgtcaaatcc tgggccagat tctggaattc ttgtgaaaaa gaaacacggt ctcgcccttc
    30721 aaagagatga gctaaactgt aggccaagga tttccgagcc tgcaaggttc catcttttaa
    30781 ttcaaattgc cccagttcct ggagattttg gcgaattttc ttggtttctt catcgaaaaa
    30841 gtaaacttga ttagacgaat caatataaaa atcctgattg gcaaccaagg cctgcatggc
    30901 ttggtcgatt tcctcctggg caatattttg aaaatcaaac tggatttcca agagacctcc
    30961 cttggaggca atctgcacct gaggactcgc taggctataa agctcttcta gtttatctga
    31021 taggtcaaca tgcccgagtt tttcaaagac tggaatgata tcatgaaaaa aatgatagac
    31081 agactccgct tttaaggcct gacgccaaga ttgaaaatcg gcttcaaagc ccgcagccaa
    31141 acagacttgg aaaattcttt cttctaagtc cgcgtcactt gaaaagggta attcttctag
    31201 ctcttgtcgg ctagatacct gtctatttcc ataatcaaac tgaatttcta aacgaatccg
    31261 attatcttct tccctgtcaa agtaaaaaga gggcgcaaaa gttttgattt gtagacgttc
    31321 tggagctgaa acggtgccca tctggataaa aagagtcaga caggaggcca atttgtctcg
    31381 atcactgcta tcaaattgca ggtatttctt tccttgttga cccacaggta acgctttaat
    31441 ttccttgaga agacgcatct gctggtctgt taaaaaataa acctgacctt tatggaaaag
    31501 tactgctcct tgataaaaga cattgaccct aggactctca ctgatttcca tttcaaaata
    31561 gtccgagtat tctgttactg taaaggcaaa tagattggca tcagcatgca tatcctgaaa
    31621 aagcagggtt tggtagctat ccacttgatg gtcaaattga aaatggggca aggccatcag
    31681 taaattcaca ccctgctcaa aaaaggtcag agggaaaaag aggtgccgac cttggttttg
    31741 gaaaaagagg tctggaacca gcccttcctc cgttagtccg tgcaagaaag tcaaaagttc
    31801 ttggctggca tcatcaaagg cttcccaaga aagagactcc tcataaatct tgccaatcat
    31861 atacgacttt ctctgctcga caatccttaa aaaaagtgga atatcccgaa tgacatagta
    31921 tttttggcta ttgatttggc cgattctcag agtccacaag atatgattgg ttcctgcttc
    31981 cacctgaccc acagctgata actcataggc gcattctgat tttggagata aaattcgatt
    32041 caaaaacttg ccacccaagg tcaccttggt ttcaacggcc tctttttctt catgaccttc
    32101 ttccagactc cacaagattt cctgaccacg ctcatcattt ttcagaaaat gctctagcgc
    32161 tgccaaatgc acacagtagc ccctcttttg aaaagaatca caggcacaaa aaaccaaatc
    32221 atcctctaaa ctatagcgca gttcttcttc tgcaacgcga gcgtagagcc gattgttctt
    32281 ttccttgatg atatcaacct taccagtttc ataaagggca acaccttcga tacgaacttt
    32341 ccccggaatc aatttagcca tattttcacc tttaccttat ctttttatta taccatattt
    32401 tcgcctatga aaatagcctt ctaggaagac ttttctccta gaagactgga tttttaacgt
    32461 ttggcaaaag taaccacaat ccgctgacag acttcctgca acagagattt gggcatagct
    32521 atattgatgc gggcatggag acttccttcc tctccaaaat ccaaaccacg gttgaggata
    32581 accttggctt catttctcaa caactcttgc aatgtttcat cagtcaggtc ataggctgaa
    32641 aagtcaagcc aaatcaagta ggtaccttgc ggtttcatga ccttgatttt agtctctttt
    32701 ccaaatagat ctaccacata attgatgtgg tcttcaaaga cttgcttgag ttcctctagc
    32761 caatctttac cgtatcgata ggcagcttct gtcgccaaat aacccaagcc tgaaatttca
    32821 tgctgattat tggccaacag gcgtttctgg aaagccagtc tcaacttagg attttcaatg
    32881 actgcatagg aattttttgt tccagcaata ttaaatgttt tagtggcact gctcaagacg
    32941 atagcaaaat ttttgaaggc aggattgatg gtattgaaag actggtgttt gtgaccaaag
    33001 agggtcaaat cttggtgaat ctcatccgaa actaacaaaa caccgtgttt ttggcagagt
    33061 tggccaatct tctccaacac ttctttttcc caaacacgtc caccaggatt gtgaggattg
    33121 caaagaatat agagtttaac ctcctcttcc accaaatcct tttcaagttg gtcaaagtca
    33181 atctcaaaca gaccatcctt ttccactaag gaattagtaa tcaatctacg attattcaac
    33241 ttgacactgc gagcaaaggg tgggtagaca ggcgtgttaa ttaaaaccgc ctcgccttct
    33301 tttgtaaagg tttgaatagc tgttgagatg gctggtacca caccctcgat aaagacaaga
    33361 gcctctttgt caaagttgta accgtattgt gtagcttccc acttttgaac ttccttaatt
    33421 aagtcttcac tggcataggt ataaccataa accagttggt ctgcgtaagt ttgcacggct
    33481 tggcggattt caggcaagac cacaaagtcc atatccgcta tccaagctgg tagaacttca
    33541 ctatccgttt ctgtttcttt ccatttatag gtatggtgcc ctaaacggtt gggcaggctt
    33601 gtaaaatcat attttcccat ctttgtctta tccttctaag gcttggcgca aatctgcaat
    33661 caaatctcta gtatcctcaa tcccaataga caaacgcaag aggtcatctg tcaaaccata
    33721 agaatggcgt acttctgctg gaatatcagc atgagtttga gtcgttggat aagtaataag
    33781 actttccact ccgcccaaac tttccgcaaa agagaagacc ttgagactgt tcaaaatatg
    33841 aggaatgcgt gtttcatcgg ctactttaaa ggaaatcatg cctccacgac cagtgtagag
    33901 aacttcctta actgctggag aatccttcaa aaaggcaacc acttcttggg cgttagctgt
    33961 tgagcgctcc atacgaagag acaaggtctt gagaccacga agcaactggt agctgtcaaa
    34021 tggagacaag actgcccctg ttgtattgag attgtaaaaa agcttctcgt atagttctaa
    34081 actattggtc acaaccactc cagccaagac atcattgtgg cctgctagat acttggttgc
    34141 tgaatggaga acgatatctg ctccatcttc aatcggacgt tggtagatag ggctatagaa
    34201 ggtattgtcc accaccactt tggcaccctt agcatgagcc aattttgcta gtttttcgat
    34261 atcaaattcc aacatcaagg gattggttgg ggtttcgata tagagaacat ccacatcctt
    34321 ttctaactca gcaatcaact cttcttctgt attggcatag gtaaaatgga aacgaccttc
    34381 ctgctccact tggttaaacc agcgaaaaga accaccgtaa agatcacgca ctgccaagac
    34441 cttacttcct actggaaagc cgctaaaggc cagtacaata gctgacatcc ctgagctagt
    34501 cgctagggca tagtctgctg actcaatagc cgccaagact tcctcagcct tactacgagt
    34561 tggattttta gtgcgcgtat agtcaaaccc agtagatcga ccaaactctg gatgctgata
    34621 ggtcgttgaa aaatgaagcg gtgtcaccaa tgcacctgtc gcttcatctg acttaatacc
    34681 cgcctgagcc aaaattgtgt taatgtgtaa ttccttgctc atacaatacc tccaaatcta
    34741 tagtaactat tgtaccactt attttcatca actcattttc ttcttttcaa gagctagtta
    34801 tagtttcaaa ctatataaaa agggagtttt tcctgctccc tttaatagac tataaaatgg
    34861 tgaatctcaa aagacacctt cactctatca tttgctcctg cacaaaacga gcataacgct
    34921 catgattttc cagtagttcc ttatgagttc ctgagccagt gattttcccc ccctctaaga
    34981 agaaaataca atccacatct tttaccgttg acaaacgatg cgctataatc acaaccgtct
    35041 tctcctttaa tgcagaatag aggatactga taatcgcata ctcagaatcc gcatcaagat
    35101 tagcagtgga ttcatcaaat ataagaattt cagcatcttt taagtaggtt ctagctattt
    35161 gaagtctttg gctttgcccc cctgacaaga atcgtccgcg ttcaccaact tcagtatcta
    35221 gtccctcttt catggagcga atctcatcac ctagtgatac taagtctagc actttcatca
    35281 attcatcatc agttactaag cgattcaaac cgagacaaag attgtcacga atactgccag
    35341 ataagactgc attattttgt gaaacccaag cgattttact tctccattct tttaagttaa
    35401 aatcatatat acttgattgc tccattagaa tatatcctga aagcggttta taaaaccgct
    35461 ctaacaaacg cacaatcgtt gattttcctg atccagatgg tccaacaaaa gcaatttttt
    35521 gccccttgaa aattgaacaa gtaatatcct ttaagacagg tcgattttca tcataaccaa
    35581 aatagacatg gttaaaattc aacccttgtc ctgataccga ttttcctcac tcaaattttt
    35641 ctttaggaac tgcaagcaag ttctccagtg caactgaaga tcccttgctc ctagaataaa
    35701 tagttacaaa attagctata ttactaatag gattaagtaa ttgaaagagg taaatcaaaa
    35761 acgaaaccaa ggttcccaca gatatatatc ctgcgctgac ccgataaccc ccataggtta
    35821 gcatcacaac tatagtcgca aagataaata agagagcaaa cggggtctca aaagaagtaa
    35881 ccctatctga tttcagtgaa ttgttttgta ccctttcaat acaattatcc aaaacatcct
    35941 gtacactttt ctctgcttgg ttagtcttaa ttaattcatg ttcttgaatc ttttcagtca
    36001 attgccctgt taaaaatccg atttcctcct gtaaacgacg actatacttt tcactgatat
    36061 tggaaagggg caagataata aacatcatac aaggaagagt gatgaataaa agtagagaaa
    36121 gattccaatc aagactaaat aagactacaa tggaaccaag taccataact aaactcagaa
    36181 taatatttgg gaaagtcgta attaaaaact cacgaatgac actcgtgtca ttgacaatgg
    36241 cagaagtcaa ctccccactt tggctcttat caaagaagga tttctctgca taaatcaacc
    36301 cctctatcac ttttttcctg atttttgcta tctttttttc acccgattga ctaaacagat
    36361 agtaaccaat agaagaaaac aaggcttgac caataaaaat caaaaacgat tgaaatactt
    36421 tggaacctat attttcaata gaactcccat ctattaaacc ctttaagata aggggaagca
    36481 acaaagcaag tagactagac agaacaagta agaaactccc cataatcacc ttagtatcta
    36541 ctcttaataa ttttaatttc ataaatactc cttataatat ttcaacggat aaagtcgaga
    36601 ataactcaat ttgaggataa aatctaataa atcttcctat aacaaaacgc ataacatcta
    36661 ggattttata tacctgatat tatgcgtttt taagcacaaa gacttcttac acaaacttat
    36721 ctacaattag attttatttg acatgttttg ccaattcttc ttgggctttt ttattggatt
    36781 cttctttttc tttcaaccat ttttctctgg cttttgcata ttcgtctgtt gtgacaatct
    36841 tatcttgtac tttgaggtat ttatatgatt caaccccttt tgtaccggtt aaaccatagg
    36901 cagcagcaaa tggtacggtt cttctcaatg atggtgttcc cccacgcgaa acacttggaa
    36961 gaactaaaga actatcaatc aaccaagctt gaatatcagc atatttctca taacgtttgg
    37021 ctggatcttg ctctttatta gcttcttcca acatttgagt atagacatcc agtccaactg
    37081 ccttagcctt gtcattggct tctccaggct ctagtccaag attttgtaga actcctccac
    37141 tattagtatt aaaaatatcg agataggttg acgggtcttg ataatcaggt ccccaaccgc
    37201 catgatataa atcatagtct ttctgagcag ctgtttgagc aaagtagcct gaactgtcaa
    37261 actcatctga tgttaattgc tgaatgtcaa tcactacatt atcagaacct aaaacagatt
    37321 caattgattg tttgatagaa ctaactcctt gtatgcctac tttatctgtt acttccacag
    37381 tcttatccaa gtggattggg aattgaacac cctttgcttc gagttctttc ttagcttccg
    37441 caaacttagc cttggctttc tcaggattgt agtaagggtc ttgaccatcc gcaaagttga
    37501 taccttgcca ttccttacca tagttgacca tcttagaggc tacaacttca ccaaagtctt
    37561 ttcccttgat actgacaaag tttggaggaa ccactaggtt acgcaaaatc tttgttgcac
    37621 cttctttccc ttcagactga gccccataag atgttctgtc aaaagcaaaa ttgatagcct
    37681 gacggaagtt tttattgaga actgcttcct gagtcgattt cttttcaatg tcacttgttt
    37741 tagaagtata attgtaagac ttcctatcta ggttaaaatt aaagaaatat gaagttgaat
    37801 tttgcatact atagatgata ttgtttttgt atttttcttt aatcccttca tagctggagc
    37861 tgttaggaaa aagacgagcc gtagtataag caccagctgt aaaattacgt tccagtgatt
    37921 cttggtcgct accatcatag taggtcaatt tcacatcgtc tacaaagaca ttcttagcat
    37981 cccagtaatt agggtttttc ttatattcaa tagcagattt tgagacaagt gctttcatca
    38041 agaaaggtcc attgtacaaa atactagatg gatccgcctt cccaaaatca tccccttttg
    38101 atttcaggaa atctgcatta acaggaaaaa gtatcgttgc aagtgttttt gaattccagt
    38161 aaagttctgg tttaaccaaa gtatattgaa ccgtttggtc atcaagtgcc ttgacaccga
    38221 cagttgaaaa gtcgcttgtt ttaccagtga tatagtcatc caaaccagca acagagtcct
    38281 gcactagata caaggcttct gattttttat cagctgcata ttgcaaacct gtcacaaaat
    38341 cctgggcagt tacaggcgca tattcttctc cctcagaagt aaaccacttg gcatccttac
    38401 gaagtttgta ggtataggtc aaaccgtcct gagaaacagt ccaatcctct gctaatgatg
    38461 gaataatatt cccatattgg tcattttcta ataacccgtc taccaaattt gcaacaatat
    38521 cggatgttgc tgcgcggttt tctgctagat agttcaagct agatggatca cttgaataaa
    38581 catagttgta ggtttttgac gccgtgctag aatttccaca cgcgctcaat aaaactcctg
    38641 tacccaggac aagacctgcc aaggttagat atttgctctt agactttttc atttccggaa
    38701 cctcctgctt taaatatttg ttttattata caactcttgg tttgtttagt caattgtttt
    38761 ttccaaaaaa tcaaaaaaac ctcattttct atttttgaaa gataatttta gctattttta
    38821 cgctaaaaaa ggaataagtt tcaagttctc ctaaaagatt gttcaactta ttccttttat
    38881 ttataaatgc taccttacga aagcttaaat tttgctcgca ggcgatctgc ttgggctttt
    38941 cctattacct tatctaataa ataggtacgc agactcataa ccatataaag tccacccccc
    39001 atggcaccga caagagctac ataaaagaag ctccacaaac gtccacttgg ttggaagaaa
    39061 aatcctaaca gccactggat ggttcctatt aacagaaaca tgactagggt cagcaaactg
    39121 attaaaatag ttcgcttcaa aatcaccttg cgcttgacac cagttacttt acaaatatcc
    39181 cgatacatca agacgttagg aatgatgaga gcaatggttg ttgaaatcaa aggaccataa
    39241 ctgtggaaga gggcgatggt aggtagttgc aagactagct tggcaataga accatagata
    39301 aaatagagaa cggccttgcg gttgcggaac atggcctgaa gcattggaga caagaccatg
    39361 tacaagccta aaataataga ctgcaaaact gcaaagacaa ataagcccag agccaaacta
    39421 tctggcttac catagaagac tgtatataga ggttctccta ccataaccac tccaaccgtt
    39481 gctggtagca agaacataaa gagtagggtg agactgtcct gaacgagacg agaagctgct
    39541 ttcaagtccc ccttgacata gttttccgtc aaaagtggca aaccaacact cccaatcgaa
    39601 acccctacag aaatcaaaat catcgtgatt ttattaggat tggctgagaa ataagaaaac
    39661 atgacaacca agtcctcatt gctgtagttg gtaaaccagc tcatactatt gataaaggtc
    39721 agctgatcca aaatctggaa gagctggatg gcagaccctg tcaggataaa aggaatggct
    39781 tccttaatgg tatcaaccaa gagacgctta ctgttaatct tatctcctgt ttcaaagact
    39841 cttttgagtg aaccttcttg ggcaaggaaa taaatcaaga ctgcaaaact ggctaccata
    39901 ccgacaaagg cagcaaaggt tgattgggta acggctgcta gataatctcc tgaaccgagc
    39961 ttcataatga taaaggttgc taggagcatc cagataacac gaatgacctg ctcagcaatt
    40021 tggctcatgg catagggttt gaggttattc atcccttgga aaaatcctcg gataacactc
    40081 atagacggga aaatcaagac tccccaagcc aagctttgca tgattgggat caagtctttg
    40141 cccacgccag acaagtctgc tagccaagga gcaaagacat acaagactaa agcaaaaacc
    40201 aggcctagtc ctgtcataaa gcctaagaaa ctccgaatca gggcaaagct atgctcttct
    40261 tctcgcatgg tattatactt ggcaacttgc ttggccaccg caactggaat ccccgctgtt
    40321 gaaaccaaca agaaccaagc atagatattg taacccatgg taaagagacc atttgcctta
    40381 gctgcataag cccccatcca gatgtaccaa gggataatgt aaacagcccc gagtaggcga
    40441 ctgataaagt tactagccgt tagccaagca gtcccccgta acatctgggc ctgctggtga
    40501 ttgttttcgt gcgacataga ttcctcattc aattttgata gctaggaaaa actccttatc
    40561 ttccattata aacttttcct tgttacttgt aaatttacga ctttcggttt acaatagaaa
    40621 gtatgattaa gattgaaacc gtattagata ttttaaagaa agatggcctt tttcgcgaaa
    40681 ttattgacca aggtcattac cactacaact acagcaaagt tatttttgat agcatcagct
    40741 acgacagccg aaaagtaaca gaagacactc tttttttcgc aaaaggcgct gcctttaaaa
    40801 aagaatacct tctttctgct ataacacaag gattagcttg gtatgtagct gaaaaggact
    40861 acgaagtcgg tatccctgtc atcattgtga acgatataaa gaaagccatg agtttgattg
    40921 ccatggagtt ctatggtaat ccacaggaaa aactcaaact ccttgccttt actggtacta
    40981 agggtaagac aacagcagcc tatttcgcct ataacatctt atctcaaggg catagacctg
    41041 ctatgttgtc gaccatgaac acaactcttg atggcgagac tttctttaag tcagcgttga
    41101 caacccctga gagtattgac ctctttgaca tgatgaatca ggctgtgcaa aatgaccgta
    41161 cccacctcat catggaagtc tccagtcaag cctatctggt caaacgtgtc tatggtctaa
    41221 cctttgatgt gggagttttc ctaaatatca gcccagacca tatcggcccg attgaacacc
    41281 ctagctttga agactatttc taccacaagc gtctcttgat ggaaaagagc cgagcagtca
    41341 tcattaacag tgacatggac cacttctcag tcttgaaaga acaggttgaa gatcaagacc
    41401 atgatttcta tggtagccaa tttgataacc aaatcgagaa ttccaaagcc tttagctttt
    41461 cagctacggg taaactcgct ggagattatg atattcaact cattggcaac ttcaaccaag
    41521 aaaatgcagt tgctgctgga cttgcttgtc tccgtctcgg agcaagtctt gaggacatca
    41581 aaaaaggcat cgctgcaacc cgcgttcctg gtcgtatgga agtcctcact cagaaaaatg
    41641 gagccaaggt cttcatcgac tatgcccaca atggggatag tctgaaaaaa ctcatcaatg
    41701 tggttgaaac tcatcaaacc ggaaagattg ctctggttct gggatcaaca ggaaacaagg
    41761 gagaaagtcg tcgtaaggac tttggcctcc tcctcaatca acaccctgag attcaagtct
    41821 ttctgactgc tgatgaccct aactatgaag acccaatggc cattgcagat gaaattagta
    41881 gctacatcaa tcatcctgtt gaaaagattg cggatcgcca agaagccatc aaggcggcaa
    41941 tggctatcac aaatcacgaa ttagatgcag ttattattgc gggtaaggga gtcgattgtt
    42001 accaaatcat ccagggcaag aaagaatcct acccaggaga tacagccgtc gcagaaaatt
    42061 atttataaga atagaaaaaa tcaagggaaa atgaaaccct tgattttttc ctattattta
    42121 gtaaaaacgt ttttcaaacc ttcaacggca ccttctacag caccttttgc gtcttctaca
    42181 acgtcttttg ccttagcaac tgttttttca acagctcctt caagttctgt cttgctgtct
    42241 ccagtaactt taccaaaacc ttctttgaca gcgcctgttg cttgttccaa tttgttttca
    42301 agtgacatat gattgtctcc tttagtttat tccgatatgt gttaccggtt acatatagtt
    42361 tataccgaat actaaggaaa gtcaaacaat gtgctcagaa acaagaaatc acgctaagta
    42421 gaaagttaat ctctccttat caaaatcgat agccaactca tactttctat cttcattttg
    42481 cacaatatag cctaagacaa ccaaactgtc cacaaagata tcacggcatt tctgcataag
    42541 ctggtctttt ttgagaaatt tcaacaaaaa agccgtcata tacttgaggg catactcagg
    42601 attaacatct cctaaaatgt catagagctc ctgctgtttt tctgtcaaag gatactgctg
    42661 tttgaccttg taaaaataat tggacagggt cattttaatg cgcgcaaagt ccgtcttttc
    42721 aactaaaata gctgcattga tttgattgcg caattccgtc tcaaaactct gctccaacaa
    42781 ggcttgatag accggactag cttctctgac aaaaatctct tgatccagtt caagactatc
    42841 aagtgattca agcgtaggaa aattgaggta atagcgctta ttttcacgta gaatcaagcc
    42901 tgcctttata tactcttcca tgagtttatc aactgcaaca tctggaaatt gagccttaat
    42961 ttcccgtaga atcacatcct catactggtc cagatagcgg atcaattctc caaaaatggc
    43021 tgtctcgtca aacgagatgg attaaaaatc tgaatcatga agatcccttt ctttacattg
    43081 taacagaggc gatgctgcca actgactagg attggtacca gcttggttca gaggaacagg
    43141 cagacctagt tctatgtaat actccctcca aaaatcacta atctcctgct ccccatctcc
    43201 aaatttcata ggaatagttt caaaaatagg taggatttct gctatatact gggagcagta
    43261 gaaaccagct gcatctggat agaaagaagc attgtagggt gctccaagat gtttgcaagc
    43321 tctttccttc accgactgga tatccatttc tgggtaaaca tagaggtcgt ataaatgatt
    43381 ggactcaaag aagtctgccg gttcttgaca gacaacacca gcctgtccac tagcatgata
    43441 aatcatccca tccaaataaa tggcaacatg gctatagtta cctgtggaag tctggatggc
    43501 ctgtcccatg tctgacccat ctctcacaaa aatcaaatcg ccattttcta acatacttca
    43561 ctcctagaaa aaaggagccg aaactccttt cgatataagg caaactagac tttccgattt
    43621 tgagctgaca cccaaaatct tacgcattaa agctttcagt caattgaggt accacttgtt
    43681 tcttacgtga aacggcacca gcaaggaagg catgattgtt ttcaagtttg aagttgaaag
    43741 ccgcttcgac cttgtccata ttggcaccaa gagccaagat ttctgagttt gagttgacga
    43801 tatctgtaat catcaagaca aagtcagagt agccgtttga ttcgttggca gcttgcattg
    43861 cagcttcaat ttctgcttgg cgttccaaaa cttcagcgat gtcaactgtg ttcacttggg
    43921 caacacggac attatttccg ttgagttcaa aagtcttagc atcgatgtca atcaattctt
    43981 cagcagattt gctagccaag ttggtaccag ctttcaacat tgctaaacca tattcttcca
    44041 agttcacacc agccaattca gccaattcag gagcaatgat tttatctgtt gggtgtgttg
    44101 ttggtgattt caaaagaagg gtatctgaaa tcaaacctga aagcatcaaa cctgcaatct
    44161 ctttaggcac agctacacca tgttctttga acatacggta aacgattgaa gacgctgatc
    44221 caactggctc caaacgcatg taaagtgggc ttgcagtttc aaagttagcc acacggtggt
    44281 ggtctacaac accataaact tctacttcag cgatatctga tacagattgt tggaattcat
    44341 tgtggtcagt caagataact tgctctgccc cctctgcttt ggcagaagtg ataatacgtg
    44401 gtgcttccac accaaaatag ttcaagacaa aggctgtttc ttcatttgga gttccaaggg
    44461 caacagcttc cgtatccaaa ccgtaagctt cttttgcaag gtaggcaaaa gctacagatg
    44521 acccgatggc atctgagtct ggattttggt gaccaaatac tagaatcttg gacatgataa
    44581 tacctcattt tgtttatttt ctttattgta gcattttttt cgctttttga caaaggtaag
    44641 aggagtcaaa ggactcccca cgattcttca aaataactga gtaaatttct atcttgattt
    44701 tcagataaaa attcttcctt ctgtggcctc ttcttacgct tgagaagagc ttcagccgac
    44761 atggcttctt ccttactggc aaaaccttga gcatagataa gtttgactgg caagcgtgct
    44821 cgtgtatatt tggctccctt cccactattg tggatagcga ggcgtcttct catatcagtc
    44881 gtatagccta tatagtagga tccatcacga cactccagaa cgtacatata agccttatga
    44941 tccataataa atctcttcga tttcgggcgt ataagagcca tcatcattgt ggacaatcaa
    45001 aggaggtaag accttaaagc cacttgttga gccatccttg atcgcctcaa tcaaaagcat
    45061 attggcttcc ttttctcttt ttggataaac aaactgcagg cgcttagggg ctagattatg
    45121 tcgttttaac gtatccaaaa tatccagaag tcgatcagga cgatgaacca tggccaaacg
    45181 cccattagac ttgagaatac tctgggcact acgacagatt tcttccaaat tagtcgtgat
    45241 ttcgtgtcga gccaagagat aatgttcact ctcgttcaga ttagaataag gatccacctt
    45301 gaaatagggt ggattacaca aaatcatatc caccttactc ccctgaatgt gagcaggcat
    45361 atttttcaaa tcatcgcaga tgacctgcat ttgctcctct aatccattca aacggacaga
    45421 gcgttcagcc atatccgcca aacgctcctg aatctcaaca gacaatatct gtgcttgagt
    45481 acgagtgcta gcaaaaagcc ccactgctcc attcccagca cagaaatcca caatcaaccc
    45541 cttcttagga aaacgtggaa atcgtgataa gagaacacta tccaccgaat agctaaaaac
    45601 ctctctattt tgaatgattt tgatatctgt cgaaaagagc tggttaatgc gctctcctga
    45661 ttttaataat tgttcttctt ccatggtcct attatagcaa attcatatta acattacaaa
    45721 aaatataaaa ctctaaacta cttcttcttt tttaaatggt gcagggcttc tccagtccag
    45781 attggtagca ttcgtcgaaa gggagcaaaa ccgtagttaa agcggtcgct tgaaaagcgt
    45841 ctccgtctag gaaactggta ctttttttcc tccaaagtgc ggatagaaag actggctttc
    45901 cccgtaaatt catctaaatc cactacctga acttgaacct cttcatcgac tttcaaggtt
    45961 tcatgaatat tttcaataaa tcctgtccga atctctgaaa tgtgaatcag ccccgtatca
    46021 cccgtctcta actcaacaaa ggcaccgtag ggctgaatcc ctgtaatacg cccctttagc
    46081 ttatcaccga ttttcatctt agtcctcgat ttcaatagtt tcaattacaa catcttcaac
    46141 tggcttgtcc atagctcctg tctcaacagc agcaatggca tccaagacag cgtaagatgc
    46201 ttcatcagct aactgaccaa aaaccgtgtg acggcggtct aggtgaggtg tcccaccttg
    46261 attggcatag atttctgcaa tcggttctgg ccaaccacca cgagtaattt ctttcttaga
    46321 ataaggtagg tgttggtttt gcacgataaa gaactggctg ccgttggtat ttggaccagc
    46381 atttgccatg gaaagagcac cacggatatt gtaaagctct tctgagaatt catcctcaaa
    46441 agattcgccg tagattgact cgccacccat accagttcca gttgggtctc caccttggat
    46501 cataaagtcc ttgataatac ggtggaaaat gacaccatca tagtagccat cttttgaaag
    46561 agatacaaag ttagccactg ttttaggagc atgttcaggg aaaagcttga tacgtaagtc
    46621 tccgtgattg gtcttaatag tcgcaagagg accttctact gtttcaatgt ctacttgtgg
    46681 aaaatgcaat tctttttcta ccataccaaa tacttctaag gcatcaaaaa tgccatcttc
    46741 ttctaatgtt tttgtaatat aatctgcttt ttctttgatt ttatcatgag aaattcccat
    46801 ggcaacgctg attccagcat aatcaaagag ttccaagtcg ttgagaccat ctccaaaaac
    46861 catgaccttc tctggtttca agccaaggtg ttccacaacc ttttccaccc ccgtcgcttt
    46921 ggagcctgaa atcggcacaa tatcagacga atgttgatgc caacgaacca tgcgaagttt
    46981 gtctgagaga ctgtcaggca agtgcaagtc atctccctta tcttcaaaag tccacatctg
    47041 atagatatct tctttttcat ggaaatcggg atctacatct aagtcgggat aaattggatt
    47101 gatagcttca ctcatcatat cggtgcgaat cgacaacttg gcatcatgac tcccaaccaa
    47161 gccatactca attccttctt gcttagccca agagatatac tcctcaacat ctgacttttc
    47221 aatctgatgc tgataaatga cctgaccttt tttatcttcg atataagccc cattcaaagt
    47281 tacaaaaaag tcaggcttga gatcacgaat ctctggaaca acaccaaaaa tgccacgtcc
    47341 agaggcgatt cctgttaaaa ttcctttttc acgcaactgt ttaaaaacag tgggaattgt
    47401 agttggaata aaccctgtct ttgaattccg caatgtatca tcaatatcaa aaaagacaat
    47461 cttgatcttc tttgccttgt atcttaattt cgcgtccatc tcactacctc tttcaatcta
    47521 actctttcca ttatatcata aagtaggcaa atcccctatt ttcaaaaagt ttatcatttt
    47581 tattttaatt tcttggatga gaaaaaagac atatttatga aaaagctcca tcgtgctttt
    47641 aatgtgttct cttgttttca aactcgtaaa aagggagcca ctgagcctaa ctcgctctct
    47701 catttcaaag tttgtgaaaa aagacccatt ggggtcttaa ttcgctttct tgttttcaag
    47761 ctcatgaaaa agagacccaa ccgggtcttt tctttaatct tcgtttacga aaggcatcaa
    47821 agccattacg cgagcgcgtt tgatagctgt tgttacttta cgttggtttt tagctgaagt
    47881 tcctgttaca cgacgaggaa ggattttccc acgttctgaa acgaaacggc taagaagctc
    47941 agtatctttg taatcaacat attcaatttt gtttgctgcg atgtaatcaa cttttttacg
    48001 gcgtttgaat ccgccacgac gttgttgagc catgtttttt ctcctttata agtttagttg
    48061 tccattagaa tggtaaatca tcatctgaaa tatccaatgg gtttgttgct ccaaatggat
    48121 tttcattacg tgaaaagtct ggtactgaat ttgtaggtgc tgaatagttt gcagttggtg
    48181 cagagtaagc tccacctgtg tgaccctcac gcacactacg gctttccaac atttggaaat
    48241 tctcagccac gacctctgtc acgtagacac gttgtccttg ctggttatcg taactacgag
    48301 tctggatacg acctgtcacc ccgataagtg agcctttttt agcccagtta gcaagatttt
    48361 cagcctgttg gcgccacata acgacattta taaaatcagc ctcacgttca ccattttgac
    48421 tcttaaatgt acggtttact gcaagagtaa aagtcgcaac tgctacattt gatggggtat
    48481 aacgcaactc agcgtcacgt gtcatacgcc ctacaagtac aacattgtta atcatagttt
    48541 accttcttac gcgtcaattt tgacgatcat gtgacgaaga atgtcagcgt tgatttttga
    48601 aagacggtca aactctttaa gagctgcatc gtcatttgct tcaacgttaa cgatgtggta
    48661 aagtccttca cggaaatctt ggatttcgta tgcaagacga cgtttttccc aagtttttga
    48721 ttcaacaaca gttgcaccgt tgtcagtcaa aatagagtca aaacgtgcta ccaaagcgtt
    48781 tttagcttct tcttcaatgt ttggacgaat gatataaaga atttcgtatt tagccattga
    48841 tatgttcctc cttttggtct aatgacccca agactttgca aggggtaagt gaggttcgct
    48901 cacaataaac tattatacta gaaaaaattt ttttacgcaa gtaaaaacac tagaattcga
    48961 aaaaacgcca catgggcgtt ttcctgttct tatggtttga tacggtgcaa catacgtggg
    49021 aatggaatag cttcacggat atgttttgtt cctgctgcga aggttaccat acgttcgata
    49081 ccgataccaa atcctccgtg tggaactgta ccgtatttac gaaggtcaag gtagaattca
    49141 tattctgtac gatccatgcc aagttcatcc atcttagcga caagggcatc gtaatcttcc
    49201 tcacgcatag acccaccgat aatttctcca tagccttctg gagcaagcaa gtctgcacaa
    49261 agcacgcgct ctggatttcc aggaactggt ttcatgtaga aggccttgat ggctgctgga
    49321 tagttcttga caaatgttgg cacaccaaag tggtttgaaa tccaagtttc gtgtggtgac
    49381 ccaaagtcat caccatgctc aagatgctcg tagtcagcat cttcatcatt ttcatgctct
    49441 tgcaagaggt caatggcttg atcgtaagtg atacgtttga atggctctgc aatgtagcgt
    49501 ttcaagagtt ctgtatcacg ttccaaggtt tccaaggctt gaggcgcgcg gtcaagaaca
    49561 ccttgtagaa gagctttcac ataagcttct tgcaagtcaa gcgactcatc atgtgtcaag
    49621 tatgagtact cagcatccat catccagaac tcagtcaagt gacggcgtgt ttttgatttt
    49681 tcagcacgga aaactggacc aaagtcaaag acacgaccaa gagccatagc ccctgcttct
    49741 aggtaaagct gacctgattg gctcaagtag gctggcgttc cgaagtagtc agtttcaaag
    49801 agttctgtag aatcttctgc cgcatttcct gaaagaattg ggctgtcaaa cttcataaaa
    49861 ccgttcttgt caaagaactc ataagttgca tagataatag cgttacggat ttgcaacaca
    49921 gctacttgct tacgagagcg tagccacaag tgacggttat ccatcaaaaa gtctgttccg
    49981 tgttcttttg gtgtgattgg gtagtcttga gattcaccga tcacttcgat gtctgtgatg
    50041 tccaactcat agccaaattt agaacgttcg tcctctttga caatacctgt cacataaaca
    50101 gacgtttctt ggctcaagcg tttgataaca tcaaacttct caagtcccac ttcttcacca
    50161 aatttttcga caaagtttgg tttaaaagcc acaccttgaa agaaggctgt tccatcacgc
    50221 aattgtaaga aagcgatttt cccttttcct gatttgttgg caacccaagc gccaatcgtc
    50281 acttcctgac caacatagtc ttttacatca ataatcgtta cacgttttgt cattattttt
    50341 ccttttcttt tttattcttt atggcaaacc acctctatat tgttcccatc caggtcaatc
    50401 ataaaagcag cataataaat cggatgctca cttcgataac caggaacccc attgtctcgc
    50461 ccacctgcct ctaagccagc ctcataacaa gcctgaactt cttccttatt ttctgctaaa
    50521 aaagcaaaat gaacaggatc ttgtgttccc tgagtcagcc aaaaatcacc accaggatga
    50581 gggctgttcg gggatagaaa actaattaga gaactagtct taaaagccaa tttatagtcc
    50641 aaaggagcga gaaaactcct ataaaatcct tctgaaattt gtaaatcctt taccttaatc
    50701 tcaaaatgat caatcattct cactacccat aaatgctttc aagcgttcga ctgcttcttt
    50761 aagcgtgtct aggtctgtcg catagctgag gcggacattt tctggtgctc caaatccagc
    50821 tcctgttacc aaggccactt cggcttcttc taagatagca gttgtaaagt ctgtcacatc
    50881 cgtgtagcct ttcatctcca tggccttttt gacatttggg aagagataga aggccccttg
    50941 tggtttgacc acttcaaatc ctggcacctc tgcaaggagg ggatagatgg tattaagacg
    51001 ttcctcaaag gcctgacgca tgctttctac agtatcttgc tcacctgata gagcctcaac
    51061 tgctgcatat tgggctactg ctgacggatt cgaagttgtt tgacctgcaa tcttggacat
    51121 ggcagcgata atgtctgctt ctccaacggc ataaccaatc cgccaaccag tcatggcata
    51181 ggttttagac acaccattga tgaccactgt ttgcttgcga atcgcttccg ataggctaga
    51241 aatcggtgtg aactcatgac cattataaac caagcggcca tagatatcat ctgctaggat
    51301 gagaatatca ttttctacag cccagtttcc aattgccaag agttcctcac gggtgtaaat
    51361 catacctgtg ggattagatg gcgaattcag caccaaaacc ttggtcttgt cagtgcgagc
    51421 tgcttctaac tgctctacgg tcaccttaaa gtgattgtct tccttagcag aaacaaagac
    51481 gggaacgcct tctgccatct tgacctgatc tccatagcta acccagtatg gggttgggat
    51541 gatgacttca tcacctggat tgaccacagc cataaagaag gtatagagag aatatttggc
    51601 tcccgcagcg actgtcactt gatttgacgc tacagaatag ccgtaaaagc gctcaaagta
    51661 gctattgacc accgccttaa gctctggcag acctgaggtt actgtataaa aagaagcacg
    51721 cccatctcga atcgatgcaa tggcggcatc ttggatattt ttgggagtag tgaaatctgg
    51781 ctcacccaag gttagagaca aaatatctct accctcagcc ttcagtgctt tggcacgggc
    51841 tccagcagcc aaagtcacac tttcttccat ttctaaaaca cggttggata gtttcatagg
    51901 ccctccttat tgaccaatgc tcctgtttca aaatctacta gataaaaatc agatcctgac
    51961 ttaacttccc agattggctt gtcttgataa cgaccaaagg ttatcttgtc aatctcgcca
    52021 gctccctttt ccttagaaac cgtttctgct ttttcttgtg aaataccctg atttagctga
    52081 taaacgtaaa tcttatggtc atctttacca atcaggacag caagcgcttc ttgctgttta
    52141 ttacgaccaa gaacgctgta ataagattcc aagccattgt ataaatcaac ctgatcagcc
    52201 tgctctaatc ctgcatactg ctgagctaat ttttctcctt cacttttagc tgtttgatag
    52261 ggtttcatgc taagagaaac catatacaga aaggaagcac tgataaccac aaacaaaatc
    52321 gtcatcccta gaccatactg ccacagtaga ttattttttg ctttgttttg tctttttttc
    52381 actcgtctat tttaccatct attaagcttt attacaagtg aatataagaa tactcttcga
    52441 aaatctcttc aaaccatgtc agctttatct gcaacctcaa agctgtgctt tgagcaacca
    52501 attctatttc tcccttcaaa caaaaccgat tttgaaagtg aaacagttct tactttttca
    52561 gtcacaaatg actagagttt ccgaaatttt acaattttca aataaactct gagcaaattt
    52621 cttgcaagtt cttttgtttt gttgtaatat attttataac aacgagagag ttctcgaaat
    52681 tttaagaaaa aggagacaca tcatgtctaa aaaagtatta tttatcgtcg gatcactacg
    52741 tcaaggttct ttcaaccacc aaatggcgct cgaagctgag aaagcacttg ctggtaaagc
    52801 ggaagttagc taccttgatt attcagccct tcctctcttc agccaagatt tggaagttcc
    52861 aacacatcca gctgtagctg ctgctcgtga agcagttctc gttgcggatg ctatctggat
    52921 tttctctcca gtctacaact tctctatccc tggtacagtg aaaaacttgc ttgactggct
    52981 atctcgtgcc cttgacttgt ctgatacacg tggcgtttct gcccttcaag acaagtttgt
    53041 cacagtatca tctgtagcca atgcagggca cgatcaactt ttcgctatct acaaagacct
    53101 cttgccattt atccgtacac aaggcgttgg tgatttcact gctgcacgtg ttaatgactc
    53161 tgcctgggca gacggaaaat tggttcttga agaaacagtc ctaaactcac ttgaaaaaca
    53221 agctcaagac ttggtcgaag ctatcaagta actaacactc aataaaaatc aaaaagcaaa
    53281 ctaggaagct agccgcaagc tactcaaagc actgctttga ggttgtagat agaactgacg
    53341 aagtcagtaa catatatacg gtaaggcgac actgacatgg cttgaagaga ttttcgaaga
    53401 gtataacata ataaaagcga acaagactac ttttctgacg ctcagaagac tagcttgttc
    53461 gcttttttct catttataaa ctaaaatagc tgatgataca tattgccaaa tacaaaatat
    53521 ctgatttcta tacttccttt acaaaaacca atccctgtgg ttgtgacaaa tcttctcggt
    53581 aaacaaatcc agcaaagttc aagcgacgtg cttcccccac agtttgtact tgattttcta
    53641 ttaaagctgt tagaaaggcc ccgcgcgctt tcttggagat agttgagtga atcttcagct
    53701 gaccgcctct atcctccatg aatttgaagg tcaccatctt tgctctgatt tccttagaaa
    53761 atacagtctc aaactctgat gacaagagag agaaaatcac ttcttccttc ttcagagctt
    53821 catcatagac tgccttccaa tggctcttca aagtcttacc agcaactttt aatttcatca
    53881 aaaaatccaa acggtgagga gccatgggtg acaaggctgg aacaacaccg tacaaagccg
    53941 aggtaatgaa gacatgattt tcaagataat cttgttccgc ctcggtcagc ttatctctct
    54001 taatgttgcg gtacataagc ccatcaaaaa gtttcaaggc tggatagtgt tgagtagttt
    54061 gccttttcaa agcttggata ttttgaaatt cttccgccgc tttctcagct gataccttgt
    54121 agaaactctc caattgactg gcagaataga gagccaaggc atcaagcacg gcctgacttt
    54181 ctggttttaa agaaattgcc tcgatacttg gcaagtctgt gttcatttct tttgctgttg
    54241 ggattaaaat tttcatattg atagtgtagc atatttttta gccacaacca agaaattcat
    54301 ttgaaaaacc tcggtttgac cgaggttttt ctatttattt gggccatcct aaccagacag
    54361 ccacgagaac aagggctgtg ccagctaaac ttgttaagat agataagaat ttatcttttt
    54421 tctccttgaa ctgagaaaaa cctatcttca aagcgagcag tccgagtagc attgaagcaa
    54481 ccatcaaata aaaacccatt tctttgtcct ccattaggct taatttacct tattataaca
    54541 tatactttct gaaaaaaacc tatttttacc gtagcttaaa ggtcttgaga taattgtctt
    54601 ttcctacttg accttcgaag gttttaccat tttcaaggta aggaaggtca tcggatactg
    54661 aagccttgac cttgtatatc ttgccatcaa ctttaaagaa gtagacagtg tctcccttga
    54721 taacagctga tttgaggtct gctactactc cattgatgct ttctgtcgtt gcattgtcaa
    54781 tttcaaggtc gtttttattg gcatacttgc tgagcatctc ttccactgta gtagcaacga
    54841 taacattttg gtactcgact gcgtctacca gggcgtactc tttgaccaag ccagcattgt
    54901 ccttcaagcc catgatgtag agaggcttgt cattgaggtt gatgaggatt gggaaggttg
    54961 ctttgtagga tttctcctga acagcacctt ctgctgattc acgggctgat tcttctgtcg
    55021 cagaagccaa gctatactta gtgatttctc ctgttcgcat attttcaagg atgaaaccaa
    55081 gattactctc atccgcatta gccgacgtca cacctgtgta gagatagatg tcattaccga
    55141 tagacaagta attatagccc ttggtagtct gggtcacgtt tttcttggaa atcatggcat
    55201 tcaagaaacc gtccttgtac ttgccgttgt agttgatttg ctcaatggtt tcctctgctg
    55261 gatagatcct gtccacccat tctggaacat ctgataagct gtattccttg gtttctccat
    55321 ttgtagcatc caagataatg actgaagcag gacgaggaac agcaagtcca aattgctttt
    55381 ggtaaaccgt tgctacatag aaaggattgc cctcatcgtc cacctcaaaa gatggagttt
    55441 taaagatttt ggtcgggtac ttcaagcgca ggtgacgttt gacatcacgg ttaaaatact
    55501 ccgagtctga atacttgatt ggtgtcttca agtccaccaa atccgcattt ccagttacca
    55561 tgtccacctt gatatactca ccgattccct tggcttgatt gttaaaccat ttgatagggt
    55621 ctgcatattc tagtggtgtg acccgataag gtttcccatc aattgtcaat tgggtatagg
    55681 tatctgccgc tacgtattgc gacaccttat cggttaggga acccaagtag cggtctccaa
    55741 ttttttcagc agtacttcta tctaggatag gaaccttact ggtgtcactc ctaggaaatt
    55801 cagtaaagtc tttttccgta accgtgacta cattggcata atttttagcc tgaaacatgc
    55861 tggaagttac caaggaaacc aaagctgcca agagaagaat tcctccaatc gaagctaaca
    55921 gaattttccc taaccgattg attttgaaac cctcaagatt taaggcagct tccgccttac
    55981 cgtggcgcac atgaaccgtt ttgacaaggt ttatcccctt gccaaagcca aataagattg
    56041 ccacaactaa caaatgccca cagaggaaga agagaaattc ccagctggtc aggttaaggg
    56101 gcggtaaaaa gatataccag gtcgttgcga taaaaataag ttcaaagact attcgtttca
    56161 tttgctttcc tcctaaatga ttcgtccttc caagtgatcc agctcatgct ggcaaatctg
    56221 agctgggaag cctgtcaagg taatggtctg ttcctgccac ttgctgtcac gataggcaag
    56281 ccttatggtt tcataacgct tagttgatct cacacctacc aaggacaaac agccttcttc
    56341 tgcctcataa gatccttcaa aggacagaag cactgggtta aacatgacca cgggaaccaa
    56401 gccaagatta aagataatca cgcgcttctg caccccaatc atattggcag ctagaccaac
    56461 acaggtatca cgatttgcta agagtgtatc ctgcaaatct ctggcaagat aaaggtcctc
    56521 ctgacttgcc ggctgagaca cttgagataa aaataagata tccttcacaa ttttcttttc
    56581 cacttcctca cactcctctt gctttttact atattatagc aattatagca caaaagccga
    56641 taactgcaaa ccctttccct caactgtagc aaaaagccat ccgaagatga cttttttgct
    56701 atttaatttc tgtataagtt acttccaatc cacgcttaac agctggacga ttggcaattt
    56761 tttctgccca ttttactaga ttttgataac ttgaggcatc caagaatttt gcagaacctt
    56821 ggtaaagatt tccttgaact aactgtccat accaagatca gatagcaata tctgcaatcg
    56881 tatagtcatt gcctgcaata taaggtttct gagccaattc cttatccaat aaatccaact
    56941 ggcgtttcac ttccatcgta aaacggttaa taggatattc caatttttca ggagcataat
    57001 tgaagaaatg tccaaatccc ccacctagaa aaggtgctgc acctgcttgc cagaatagcc
    57061 aattcaaaac ttctaccttt tccacaggat tacttggtaa aaaggctcca aatttctcag
    57121 caaggtaaag aagaatatga gcagactcaa agactcttac gctttcagta cctgactggt
    57181 ccaataaggc tggaatcttg gaatttggat tgagcttcac aaagtctgag ccgaattgat
    57241 ccccatccat gatagcaatc ttatacaagt cgtaagccgc ttccttaaaa ccagcttcta
    57301 gtaattcttc caataagata gtaaccttca caccatttgg tgttcccagt gaataaagct
    57361 gaaaagcttg ttctcctttt ggcaagtttt gttcgaaacg ggcacctgct gttggtctgt
    57421 ttagccccgt aaaagctcct tgattactag cttcatcctg ccatacggtc ggtaattgat
    57481 atgctgacat ccgaaacctc ccttaaatcg cattcttgtc aaaaccgagt ttgcgttgaa
    57541 taaacttaac gatttcgacg atgataatca ttgagaagct tccagccata acaattcccc
    57601 attgtgacaa gtctagtttg gttacgtgga agattccttc aagcggttct acaacgattg
    57661 ttgccatgag aaggataaag gataccaaga tggaccagtt aaaggtctta gacttgaatg
    57721 ggccaactgt caagatggat tggtagacag acttgacatt gtaggcatgg aagagctgaa
    57781 tcaaaccaag ggttgcaaag gccatcgtta gggcatctgc atgaatagca tgattgtcac
    57841 ccacatgaac tgggtaagca atcgcaaggc cataaacact cataacaaga gctgcttgga
    57901 gtacaccttg ataaatgata gaactcaaaa caccacctga gaagaagctt gccttgcgtc
    57961 cacgtggttt atgattcatg acgccaggtt ctgcaggttc aacaccaaga gcgatagctg
    58021 ggaaggtatc cgttaccaag ttgatccaca aaagatgaac cggctgtaag acatcccaac
    58081 caaacaaggt tgataggaag atggttaata cttcagcagt attagcagaa agtaggtact
    58141 gaatagtctt ttgaatgttt gagaagacct tacgtccttc ttccactgcg acgataatag
    58201 tcgcaaagtt atcatctgca agaatcatat cagaagcccc cttagaaacc tctgtaccag
    58261 tgattcccat accgataccg atatcggctg ttttcagagc tggcgcgtca ttgacaccgt
    58321 cacctgtcat ggcaacgact ttaccttgtt tttgccaagc cttgacgata cgaaccttgt
    58381 gttctggaga cacacgggca taaacagagt attgaccaac gactttttca aattcttcat
    58441 ctgacagttc attgagttca gcaccagtta aaacgtgacc ttctgtatcg tttgcgtcaa
    58501 tgattcccaa acgtttggca atggcttccg ctgtgtcttg gtggtcacct gtaatcataa
    58561 ttggacggat tcccgcttcc ttagccacac gaacagcctc agcggcttca ggacgttcag
    58621 ggtcaatcat cccaatcaaa ccagtaaaaa ttaaatcatt ttcaagctct tcagaagtga
    58681 gattttctgg aatactatcg ataatcttat aagcacctgc aaggacacgc aaggcttgat
    58741 gagccatttc agaattgttt gtatgaatga gatttgtaac cttctcatca atcggagcaa
    58801 tatccccagc cttatcacga agaagacaac gttttaagag ttggtctggc gcacccttga
    58861 ctgctacaag gaaacgacca tctggcaatg ggtgaactgt tgacatgagc ttacggtcag
    58921 agtcaaatgg caattcagct acacgaggat atttctctaa gaaacctttg acatcatagc
    58981 ccttgtccaa ggcatattgg ataaaggctg tttcggttgg gtcaccaatc aagttacctt
    59041 ccacatcgat tttcgtatca ttggccaaga caactgaacg aagtagtggc atttcaagac
    59101 ctagttcaat atcatcagct gagtcatgta gaaccgcatc gtagaagact ttttcgactg
    59161 tcatcttgtt catagtcagc gtaccagtct tatcagaagc gatgatttca gttgaaccaa
    59221 gtgtttcaac tgctggcaac ttacgaacga tggaatgtcg tttggccaaa acttgagtac
    59281 caagagaaag aacgatggta acgatagcag gaagtccttc tggaatggct gcaacggcaa
    59341 gggcaacaga agtcaacaac tcaccaagtg gatttttccc ttgaatgaag acacccacta
    59401 caaaagtaac aagggcaatg accaagatag cataggtcaa gaccttagaa aggttgttca
    59461 agttttgttt gagtggtgta tcagtctcat ccgcatcttg aagcatacca gcaatatgac
    59521 caacttcagt gtacatacct gtattgacaa caacacccat cccacgacca taggttacgt
    59581 ttgagttttg gaaggccatg ttgacacggt caccaatacc agcatctgtc gcaagctcga
    59641 ctgacaagtc tttttcgact ggtacagatt cacctgtcaa ggctgcttct tcaattttaa
    59701 gagagttggc ttctatcaaa cgtaggtccg ctggtaccac gtcacctgct tcaagggcaa
    59761 cgatatcgcc tggtaccaat tctttagagt caatctctgc catgtgtcca tcacgaagaa
    59821 cgcgggcaac tggactagac atggatttga gggcttcaat agcttcttca gcttttcctt
    59881 cttggtaaac accaaaggca gcgttgatga taaccacagc taggatgata atggcatctg
    59941 cgatatcttc cccaccagaa gtcacgactg acaagattgc tgccgcaact aggatgataa
    60001 tcatcaaatc cttaaattgc tcgatgaatt tgaccaagat tgatcgtttc tcgccttctt
    60061 cgagttcatt gtgcccaaat tcggcaaggc gcttttccgc ctcacttgat gacaaacctt
    60121 gctcggtcgc atccacagcc tgcaagacct cttcagggct ctgagtataa aacgcttggc
    60181 gtttttgttc ttttgacatg tgtctcctcc ttgacattgt gtgcaaaaca gactctcttt
    60241 ctgtcatatc ttttcacgac aaacaaaaag agacctgtta atcataacaa gtctcgctgt
    60301 ttaagatagg gccggaaagc atacttttca gcataaaatt cggaatgacg acactatcac
    60361 aggtttctgc cagctactcc cttgagtagt accattatac caaattttgg ggagttttca
    60421 aagagtaaaa actgccttat ttgaattttt ccttgaaaac cagtataatg gtagaatgct
    60481 atgtgactag aaaggaagtt gaatgaagca atctatctca aatctcaagt tagctgagcg
    60541 tggagccatt atcagtattt cgacctattt gatcttgtct gcagctaaat tagcagctgg
    60601 tcatctcctt cattcatcca gtttggtggc cgatggtttt aataacgtat cggacatcat
    60661 tggaaatgtg gccctcttaa tcgggattcg gatggcgcgc cagcctgcag accgtgacca
    60721 ccgttttggt cattggaaga ttgaagattt ggcaagcttg atcacttcta tcatcatgtt
    60781 ctatgtcggt ttcgatgttc taagagatac cattcaaaag attctcagtc gggaagaaac
    60841 ggtcattgat cctcttggtg caactctagg aatcatttct gcagcgatta tgtttgtggt
    60901 ctatctctac aatactcgcc tcagtaagaa atccaactcc aaggcgctga aggcagctgc
    60961 taaggacaat ctttctgacg ctgttacctc acttggaacc gccattgcca tcctagctag
    61021 tagtttcaat tatccgattg tggataaact ggttgctatc atcatcactt tctttatctt
    61081 gaagactgcc tatgatatct tcatcgagtc ttcctttagt ctttcagatg gctttgacga
    61141 ccgcctgctc gaggactacc aaaaggctat catggaaatt cccaaaatca gcaaggtcaa
    61201 atcgcaaaga ggtcgcacct acggtagcaa catctacctg gatattacac tagagatgaa
    61261 tcctgacttg tctgtttttg aaagccatga aatcgcggat caggtcgagt ctatgctgga
    61321 ggagcgtttt ggcgtctttg ataccgatgt ccatatcgaa ccagcaccta tccctgagga
    61381 tgaaatttta gacaatgtct ataaaaaatt gcttatgcgt gaacaattga ttgaccaagg
    61441 aaaccaacta gaagaactct tgactgatga ttttgtctat attcgccaag atggagagca
    61501 gatggataaa gaggcttata agaccaaaaa agagttaaat tctgctataa aggacattca
    61561 aattacttcc atcagtcaaa aaaccaaact catctgctat gagttagatg gtatcatcca
    61621 taccagtatc tggcgtcgcc acgaaacctg gcaaaatatc tttcatcaag aaaccaaaaa
    61681 agaatagaga aatcctttca tgagacggga tttttctatt cttttatact caataaaaat
    61741 caaagtgcaa attaggaagc cggtcacagg ctgtacttga gtcggcaatg tgaagccgac
    61801 atagtttgca ctttgatttt cgaatagtct taactatcaa attcactgag atactcatag
    61861 cgttcgtatt tttcaaggag tgcttcattt ttctcatcca attctttttg gagagtagtc
    61921 agcttaccaa agtcagagcc gttagcctgc atttcctctt caatagcagc gatacgtttt
    61981 tccaaggttt caatatcacc ttcaatactt gcccactcct gcttttcttg gtaggtcatg
    62041 cgtttcttgt cttctcgaac cttgaccact ttttcctttt cgaccttttg cacttgattg
    62101 gccatatctg tttcaaaagc tttttcatca agatagtcgg tgtaatgacc aaagaaagga
    62161 cgaatcttgc catcctcaaa agcgagaatc ttggtcgcta ccttatccaa gaaatagcgg
    62221 tcgtgactga ctgttaaaac gggacctgca aaaccttgca agaaattctc taagactgtc
    62281 aaagttgcaa tatctaggtc attggttggc tcgtctaaaa gaagaacatt tggtttttcc
    62341 aaaagcagtt tgaggagata aagacgtttt ttctcacccc ctgacaattt ctcaatcaag
    62401 gttccatgcg tcgaacgtgg gaaaaggaac tgctccagca actctgcaat cgaagtcgta
    62461 gaaccgccac tggtcttgac ctcttctgcc acttcctgca ggtaattgat aactctcttg
    62521 ctttcatcca aaccctcaat ttgctgagag aaataggcga tgcgaacagt ttccccaatc
    62581 acaacttgtc ctttagttgg ctcaagactt cctgcaatca agttgagcag ggttgatttt
    62641 ccaacaccat tgtccccaac gattccgata cggtctttgg cctgcaccaa gagattaaaa
    62701 tcttgcaaaa taggcttgtt ttcataggca aaggaaacag cctgaaactc gatgactttc
    62761 ttcccaatcc gactggtttc aaagttcatg gtcaagtctg tctcagcact actgcctgaa
    62821 acttcctttt tcagatcatg gaatcgattg atacgggcct gctgcttggt cgcacgcgcc
    62881 tgcggttgtc tgcgcatcca ggccaattct tgtttgtaga gttgttcttt tttgtgaaga
    62941 agagccgcgt cgcgctcatc ctgttccgcc tttaggcgaa catagtcctg gtaatttccc
    63001 tggtactcgg tcaagcctgc tcgctccaac tcgaaaatcc gtgttgacaa agcgtctaag
    63061 aaataacgat cgtgagtgat aaaaaggacg gtcttcttag aatttttcaa aaagagggtc
    63121 agccactcaa taatcgcaat atccagatgg ttggtcggct catccaaaag caaaaggtcg
    63181 tggttgccaa gtaagacttg tgccaactgt acccgtcttc tcagaccacc tgacaattcc
    63241 ccaacaggag tagataagtc ttgaatgccc aatttgctaa gaacggtctt gacctgactc
    63301 tcgatttccc aagcttggag agagtccatc tctgccatga cacgttccaa acgcgcctgc
    63361 ttgtcctcac tatagtcgag cataatcaat tcatactcac gaatgagctg gatttccttg
    63421 agttcactag atagaaccgt atccaaaact gtctttctat catcaaaatc aggatcctga
    63481 gtcaagtaac caatctggta atcattttta gctgaaaaag gactgacatc cccatcaaat
    63541 ccagaaacac cagaaaggac gtccaaaagg gtggtcttgc cagtcccatt gacaccgatt
    63601 aaaccaattc tgtctaagtc atggataata aaggaaatat ccctaaaaac ggtcttgtca
    63661 ccaacggatt tacttagttt ttcaacgata aaatcactca ttttttctcc ctcaggtaag
    63721 catggatggc ttcacgatta ttctccaatt ctccatcgac aatggcaaac tcaatctctg
    63781 ttaaaatctc tcccaagtct gggcctggct gatagccata ttccttgatc aaaataccgc
    63841 cgttgatttg gatttctttc ttatcatgaa tagtcaagct ctggtaggtt tctttaatga
    63901 cttgtgggtt gacttctttt ccttgagctt gacgaagatt ttcagcctgt aaaagtaaat
    63961 ccaagtcaaa gcgataacaa tcacgcttac tcaattctcc attttcacgc agagccaaaa
    64021 taatcagcaa atcctgaact tgcttggcaa actggcgtga ggtcttccaa gatttcaaaa
    64081 atgactgcgc attttcaatc tccaaagccc atagtaaagc cgcccaggct tgttcagagg
    64141 attcaaaagt aaaatcagtc tccaaatcaa acagtctgtt gagcttgtcc tggctagatg
    64201 ccatatcagg gagatagtca taagcttgac tctcaatcat ggaagccaag ccccttctcc
    64261 aaaatggagc cagcaagagt ttatcaaact cgacgaaggt acgctctaca gaaattttct
    64321 ccaaaagcgg cgtcaaggtc ttcatagctt taaatgtttc tggctcaagt gcaaaaccaa
    64381 gactagcctg aaaacggaaa ccacgcataa tccgtaaagc atcttcgttg aaacgctcac
    64441 tagccactcc aactgctcgc aagacttgct tttccaaatc ttctaaacca tggaacaagt
    64501 caacgatttc tcctgtctca tccaaggcaa aggcgttgac tgtgaaatca cggcgtttga
    64561 ggtcttcttc tagcgagcgc acaaaggaaa ccgcactggg tctgcgatag tccacataga
    64621 catcctctgt ccgaaaggtt gttacctcat actcctcatc cccatctaag accaagacgg
    64681 ttccatgctc gattccgata tcggctgttc gcggaaaaat ctgcttggtc tcttctggat
    64741 aagaagacgt cgcaatatcc acatcgtgga tagggctatg gagaagggca tctcgaacag
    64801 agcccccaac aaaataagcc tcaaagcctg cttctttaat tttttctaat actggtaaag
    64861 ccttctgaaa ttcagaaggc atttgcatta atctcataat aagtgttcta atccatagac
    64921 aagctcatga cgcttgacaa cttctttaat tcccaaattg actcctgtca tgaaggagat
    64981 gcgatcatag gagtcatgac ggagggtcaa cccttctccc tgattgccaa agatgacttc
    65041 ctgatgagct accaagcctg gcaaacgaac tgagtggatg cgcataccat caaagtcagc
    65101 accacgagca ccagcaatca gctcttcctc atctgctgca ccttgctgaa tggactctcg
    65161 aacctctgcc atcaactcag ctgttttaat ggctgttcca ctcggagcat cctttttctt
    65221 gtcatgatgg agctcaataa tctccacatt tgggaaatat ttggcagcct gcgtcgcaaa
    65281 ttgcatgagt aagacagcac ccaaggcaaa gttaggggca atcaggccac ccaagtcttg
    65341 ggcacgagaa aattctttta gctctgcaat ttcttcactc gtgaaaccag tcgttccaac
    65401 tactggagca aagccatttt caagagcaaa acgtgtattt tcgtaggcaa cagctggagt
    65461 agtaaaatct acccagacat ccgcttcaaa accagctaaa tcagccttat ccttgaaaac
    65521 aggaataccc tgccattctg actcagactc aaaaggatcc aaaactgcca ccaagtccaa
    65581 gtctggatca gtcaatacca tctgacaagc agcctggccc atctttccct taaaaccggc
    65641 aataattact cgaatactca tctctactcc tgtctaagat acaaagtccg taagaacaca
    65701 aagtgaaaat aggaattcca atcaagaagt gtctacttct tggaagaact atctttttca
    65761 cacagggttc caggcgtgtt caattatcaa gatacaaagg accttagctg cctctgaaaa
    65821 atagggaatg gcactgactt tccacgaaag gcaagacagg catctttttt caagaggcag
    65881 gtagtccgtg ttcaatttct aagatacaag gcatcttaac tagcctagaa gcgccaacta
    65941 aatcactgga atataaccca gagcaatact tcctgctcct aggtgcgttc caatgacact
    66001 accaaatgta gcaagtgaaa catccgaacc caagccaaaa tcaagcaagt gctgacgcaa
    66061 ttcttcagcc ttttcaggag cattcccatg aatgacaatg acccggtatt gacctgaagc
    66121 cgttgtttcc ttgataattt caattaagcg cttggtggcc ttcttttcag tacgaacttt
    66181 ttcgtaaact tcaatcacac cttgatcgtt aaaataaagg attggcttaa tgctaagcaa
    66241 attgcccaaa atggcagccc catttgaaag gcgtccacct tttaccaaat gatccaagtc
    66301 atctaccatg ataaaggctg acgtacggct gatttgaatg gctagcttat cctgaatgct
    66361 ggcaaaatca tcgccctgat cacgccaatt aaagacgctt tcaaccatga tgcctagggg
    66421 agcacttgta atcaaagtgt ctgggaaagc aatggttaag ccctcatagt catcgaccat
    66481 atactggata ttttggtaaa aacctgaaat tccagaagat aggaaaagcc ccaaggcatg
    66541 tgtatagcct tgttctttga gcgaagttaa gatttcatct aacttggcaa tacttggttg
    66601 actggtctta ggaaattcag aagcctgagc cattttttgg taaaattcct cagcagacag
    66661 attgatgcct tcgacatatt cctcaccatc aatattgaca ggaatatcca agacaaacaa
    66721 gtcttctctt tgcaaggtct ctgcactgag ataagcagag gaatctgtga aaacagctaa
    66781 tttcatatta gaactccaaa ttaattcctg gtaagtctaa tgcaatttca gtcacttcgt
    66841 aagtcaaacg attgagcatg ttcaaacatg gacgagccaa ggtttccacc tcttcttggt
    66901 tcaattcact tggttcattg acaatacggc catcgatatg gtttacttgt gagattgttc
    66961 cactaatgac aaacttatca aatacaatca taaagctcaa gatgacaatc aaggaagtca
    67021 cttgattttc ttggtcatgt tggagcaatt ggaaattcac atccaccttg gtttcaggag
    67081 ctccattttc attttcccat tcaaaattac gcgcatcaaa atgatactga ctaacaaatt
    67141 cttgttcacg tttaagattc atgtctttct ccatcggcta caatattata agctattgta
    67201 tcataatttt ttattttcat ctagttttct aggatttagt caatcccaat ttcagcacga
    67261 actacatctg tgatggtatc aacatagtag tctacttctt ctgttgtagg cgcttctgcc
    67321 ataacacgca agaggggctc tgttccactt ggacgaacaa ggatacggcc gttccccgcc
    67381 atttcttctt ccatcttctc gatgatggcc ttgatagctg gcacttccat ggccttttcc
    67441 ttcatgacgt tttccactcg gatattaact aatttttgtg gataaatcgt tacttctgcc
    67501 gccaactctg ataagctctt accagtttcc ttcatgattt tagtcaattg aactgctgat
    67561 aattgaccat cacctgtggt attgtaatcc atcaagataa cgtgaccaga ctgttcacca
    67621 ccaaggttgt agcctgattt tctcatttct tcaacaacgt agcggtcacc aactgcagta
    67681 actgccttgt taataccttc gcgattcaag gccttgtgga aaccaaggtt agacataaca
    67741 gttgtcacaa ttgtattttg agccaattgt cctttttcag aaaggtattt tccgatgatg
    67801 tacataatct tgtcaccatc gacgatgtca ccattctcat caacagcaat caagcggtca
    67861 ctgtctccat caaaggccaa accaatagct gacccacttt ctttgaccac ttcttgaagg
    67921 gcttctggat gtgttgaacc aacattaagg ttgatgttaa gaccgtctgg tgtttccccg
    67981 ataaccgtca attgggcacc aaggtctgca aagatttgac gggcactggt agaagctgct
    68041 ccattagctg tatccaaggc aaccttcatt ccatcaagag gagttccagt tgaaacaaga
    68101 tatccttcat acttacgcaa gccttctgga taatctacca agattcctaa gccttctgca
    68161 cttggacgag gaagagtgtc ttcctcagca tctagcaagg cttcaatttc tgcttctttt
    68221 tcatcatcta gtttgaagcc atcaccgcca aagaacttga ttccgttatc aagggctggg
    68281 ttgtggctag cagaaatcat gacaccggca cttgctcctt cagtttcaac caagtaagct
    68341 actgctggtg ttgcaaggac accaagtttg tatacgtgaa tccctactga aaggagacct
    68401 gccaccaagg ccgattccag catttcccct gaaatacgtg tgtcacgtcc tacaaagact
    68461 ttcggcgctt ccgtttcatg ttgactaaga acatagcctc caaaacgtcc tagtttaaag
    68521 gctaattctg gtgttagttc taggttagct tctccacgga ctccatcagt cccaaaatat
    68581 ttacccattg ttataaaatc cttttctatt tttaattcgt ttttgaacta gttgctttcg
    68641 ttgacgaaga tgtctccgat gaactgcttg tacttgaatt tgatgtgctt gaacttggtg
    68701 ctactggttt tgtagtcacc ttcattattg tatcaaacgg agtgataact gccggtaaga
    68761 caacaccatt gcggtcgatt gcctgcaaag gtactgaacc actgtaatta cctgttatac
    68821 gttcgctagt tggcaagaca gcgataatct tatcaattct atccaatgtc tcttggtcac
    68881 tcgtaataga cacttcttta tctgacacca tgacattttc aatttgtacc cgactatcaa
    68941 tttgactagg gtcaatctct ggtacaatct ttaccttatc cttctgagcc ttcttaccaa
    69001 tcttgactgt aattttttgc ggagtcgcca cagcggtcag cccattgggt aaatcttcaa
    69061 tgctcaaagg aacttcaatc gttccaacac cggcatctgt taggtcagca gtaaccttga
    69121 atttacgtgt actttcttgc atttcactag ctagcgatag gcgatttgca ccagtcaaga
    69181 ccactgatac ttctgaagca aaaccgctaa taaaatactt atcactatta tagcgtatgt
    69241 caatagggac atttgttact gtattagtat aggtttccgt ttttacctgc ctagcactgg
    69301 tactgttttg aaaattcgtc gccgtagcat agacaaataa gacacaagca aaaaagagtg
    69361 aggatatgat atataaacta ttttttttca tgtttccatc ctcctagcaa tcgttcttta
    69421 aaactaagac ccacttcctc ttttggaagt aagatttcac gtaattctgt ttcaaattca
    69481 tcaagtgtta ggttgtgctt aaaccttcca ttataggtta tcgaaattcc tcccgtttcc
    69541 tctgatacga caaaagtcaa ggcatctgag acttctgata aaccgatagc cgcccggtgt
    69601 ctggtcccaa attccttgga aatccctgtg ttttttgtca agggcagata ggcagacgtc
    69661 acagcgatac gttcttcttt gataatcacc gcaccatcat gtaggggagt gttgggaata
    69721 aaaatgttaa tgagaagttc tgcagaaatc ttagcatcca agggaattcc tgtcgaaata
    69781 tactcctgca aggtacgtac acgctgaata gcaaccaagg ccccgatttt acgaggactc
    69841 atgtattcaa cagacttaac aaaggcacga atcatctgtt cctcagcact aataggggca
    69901 ttggaaaaaa aatctgtcgc tcttcccaaa cgttccaaac cagtccgaat ctctggagag
    69961 aagataacaa ccgccgcaat aaccccataa gtaataattt gattgattaa ccaagaaatc
    70021 gtagtcaaac caatcatatt tgcaaggatt tgagctaaaa taaacaccaa aactccacgt
    70081 accaaaatca taatcttggt tcctgcaata gcttttgtaa aatggtataa aatataagca
    70141 acaatcaaaa tatcaatcag attgatagct atcgtccatg gacttgcaaa caaactggtc
    70201 caatattgca gattggataa ttgttgaaaa ttcatccctg atatcctccc tatcaaaaca
    70261 ctttcgtcct attataccat tttctggcat ttttttccct atcctagtcc attttacatt
    70321 gaacaaaaat atgataaaat aaactgacta gaaaaaacaa aggagaaact atgtctcaac
    70381 tctatgatat taccattgtg ggtggtggtc ctgtcgggct ttttgcagcc ttttatgccc
    70441 acctacgcca agccaaggtt caaatcatcg actctcttcc ccagctaggt ggacaacctg
    70501 ctattctcta ccctgaaaag gaaatcctag acgtaccagg cttcccaaac ctgactggag
    70561 aagagttgac taaccgcttg attgaacagc taaatggatt tgatacccct attcatctca
    70621 atgaaacggt tcttgagatt gacaaacaag aagaagaatt tgccatcaca acttctaaag
    70681 gaagtcacct gactaaaaca gttatcatcg ctatgggtgg cggtgccttc aaaccacgtc
    70741 cgctggaact tgaaagtgtt gagggctatg aaaatatcca ctaccacgtt tctaacattc
    70801 agcaatacgc tggtaagaaa gtgacgattc ttggcggggg agactcggct gtggattgga
    70861 ctttggcttt tgaaaaaatc gcaccaacta cccttgttca ccgcagagat aatttccgtg
    70921 ccttggaaca cagtgttcaa gccttgcaag aatcatctgt aaccatcaag acaccattcg
    70981 tccctagcca actccttgga gatggaaaaa cgcttgataa acttgaaatc acaaaagtca
    71041 aatctgatga aactgaaacc attgacctag accacctctt tgtcaactat ggtttcaaat
    71101 cttctgtcgg taaccttaaa aactgggggc tcgacctcaa ccgtcacaag attatcgtca
    71161 acagcaaaca agaatccagc caagcaggta tctatgctat cggtgactgc tgctactatg
    71221 acggaaaaat tgatctgatt gcgacaggtc taggggaagc tccaactgct gtcaacaacg
    71281 ctatcaacta cattgaccct gaacaaaaag tacaaccaaa acactctacc agtttataaa
    71341 aaagaaccac gagtcacata ggattcgtgg ttttataatt catccgcatt taaaaatacc
    71401 tttacatcgt tttactatat cttacaatac cttttaaaac agctatattt tgagctttta
    71461 aataaatgtt tttacatcat tttacagaga tttacgacac ttttgcccct tttttgcccc
    71521 ttttttaaaa caaaaaaacc gcaagcctga gcctgcggtg aaagaacaat ttagaaagtt
    71581 tcctttctat ttatttaact gtaatcaagc catctggctc tactgtgaag tctggcttat
    71641 ctgccagtgt tccgtctggt ttgaggtagt accagcctgt tccgtctgcg gactggacga
    71701 aggcgtttga taccatagca ccttctttag cgtctaagta gtaccaagtg ttcttgtact
    71761 tgacccagcc tgtcttcatg gcaccttcta catcaaaata gtaccacttc tcagcgattt
    71821 tcttccagcc tgtggccatt tcgcctgatt ggtcaaagta gtaccaatta ccgtctgtgt
    71881 gcttcttcca gcggtctgaa agcatatagc ctgagccatc gaaataatac caggtaccgt
    71941 tgattttctc aaacttatct tttggataag agccgtctga gtgtacgtac cagtagccag
    72001 tgccattttt ctgccagcct gttgcagctc tcaagccgtt ttcgatgtct tgcttaaact
    72061 gttcacggct aatgccccat ttagcaagat atggatatgg atccacatgg tctgagtggt
    72121 tgtttggttg gttattggta cagtattcat gcgtcttgat acctgccaag tcgtctgtat
    72181 caagagtctt cggcaagcct gcttcgtccg ctagattgcg tagcaattcg atatagaggc
    72241 ggtagtccgt catgaactct tctttagttg aatggctttc aatcagttca accgctgcgt
    72301 aactctcagc attccaaccg cccccaacat cccaacttcc gttgttcaca ggtcctacct
    72361 gcatgatgcg accgttccca acaacgtgcg agaaaaaacc taattctggg tctttccgcc
    72421 agtgataatc cgcttcattc tgtacggttg agttacggtt gcctgttgaa tgggcgtgta
    72481 cttgtcgata aggctgcacc ccaacctgtg gcaagcctgt acgtagtctg tttctatcga
    72541 tatccattcc ctatcgtcct ttccatgcgt cattcatctg cttcactgct gactctacga
    72601 aggtgtctaa gtctttgtca gtcatgctaa tattgtattt tgtaagctca gcacggactt
    72661 tagcgcgagc ctgtgccagt ttttcatctc ccttgtagcc agtttcagca gctacttgct
    72721 caacggcatt tactgcattt ttggccaaga tttcaacgat tttgatggtc ttttctccac
    72781 ctttttgaac caggtagtcc ttgactgcct tgactgcgat accagccaaa ataacaagga
    72841 tgctgattgc tccattagta atgatttcag taatttgttg catttgttat tctccttttt
    72901 cgatttcttc catgcggtcg ttcatgcgaa ccatttcttt ttgaatgtct ccgaccgcgt
    72961 gagtgattgt ggttaattct gtagtggtct tttctaggtg agtcatcaaa cgctcttctc
    73021 gtctattaga gtcggccttt gattgctcgt gcaaatccat aatcttcttc tctcgcttgt
    73081 ccgaagtctt gataagatat cgaatgataa taaagaaaag caagataaac aaaatcgccc
    73141 aagctacctg actttgagcg attttttcag cttcttcaat tggcatacac acctcctatt
    73201 ctttaggttt taccgttgga tccgtccagt caggattgcc ctctgcatca aatttcatga
    73261 tatagaattc atgattcaac agaacggcga cgttgattgt tgcgattgta ccaccccact
    73321 ggttgaacgc ccaaacggtt tcaacatcct tgagttggcg acggccattt acgatcacag
    73381 gacgtttttg aacatcacga tacatataga agtcatcgct tacattcttg caacgaatga
    73441 actctccatt ttctttcatg tagcgcaaag cactcgcaag atcaaatggt tctgtgattt
    73501 ttgtaaggtc tagtaagtta tctgtgtttt gatttgtttc tgccatgtct attctccttt
    73561 gtctgctggt ttagtttgtt catcaagcag agcttccagc tcatccactc gtgcttgaag
    73621 tctttgattc ccttcccttt gctcatccaa ctgaatactc aagatattat ttgtaatcat
    73681 cgaatttgtt gaagttgttg acatttcact aattgtcatt tgtaaggctt ggttaagctg
    73741 ttctgtgttc attttctaag ttctccaatc tgtgtgttcg ttttctattt tcaagagcaa
    73801 gctcctgaat tgctttaaag tgcgatattg gtcaacatgt tgttattcat gctattgttt
    73861 tctccatttt ttctattttt tgatttaatt cttgaatagc cttgattaag taaggaacta
    73921 aagcggtata gtcaatatgc agatagccat ctggattctc aggatctcgt gagacaattc
    73981 ttggaacgat ggtttcagcc tcttgagcta ttagaccaat ctcctcatgt ttcttatttt
    74041 cgatgaaatc aaatgcaacc attcttaatc tgttgatttt gtccaaggct ttcacagctg
    74101 tatctgtgat gttctctttt aagcgtctgt ctgatttttg ttccatccaa tacttcacgc
    74161 taccgctacc gacctgattc caccaaacaa ccgcattcct tccgcctttg ggattccaac
    74221 catcaccaag cacatcttta cttccaagtt cgataccatt tgaaaacaca ggagaacgag
    74281 aaaaagtagt attcccatag aagtttgctc tcgatgaatt cgaaaaatcc acttgatcat
    74341 aaaaaccgac ttcattccta cagtacattt tcccatcagt attgacgttc catgctttag
    74401 gtccggcata gttccaatta tttccccagt tcgcccagaa ggctgtccgg actccatgcc
    74461 cggcaccatt ccccatacca acagagaact gattgacacc tgaaatccag cgaccgccac
    74521 cctggtcaaa ttgaccaagt gtgaatccac caattcgacc ttgataggct tctaggaagg
    74581 ttgaactaga aatgacggac tcaaccttag tagagaagat acgtttagat gtcagtcggt
    74641 caataaaagc atcatttgca gttaattttc taataagcgc atcgtcaact ttcaacttct
    74701 cagcagttac cgcttcagcg tctaatatcg tagtcgtgac cgaaccagct tcaaaattgc
    74761 ccgttttcag cttatcaacc atggcagact tgatgactgc tctgtcaatc agggtctctc
    74821 cagtgatgtg ggtcaatttc ccaacgaagc ggttatgtcc attggcgcca agattgattc
    74881 cagagatgat atctccagcc gagttgatgt tttcaactac ccatgagcca gctagttgag
    74941 tcatttttgt ttgcgttgct tcaagcttct tattcgcatc tgcgactgca tcttctggat
    75001 gtggttgcca tgttctaggt ttataacctt tgtacaagtc aacttctgta atatacaaat
    75061 cagctgttcc tgatgatgag ccattgttat caaaacgaat gtaagcatta tccatttctc
    75121 cggaattaaa agttactgag acatcttcgc atctagaggt agatagtttc ttgctgctaa
    75181 caactttctt aacgattgtg aatccatcgc tctcgcctgc tcttcgtccc aaaatataaa
    75241 catcatagct tgcgagagca ctgttgttaa atcctctaaa attcagtaca tagtcagtat
    75301 ttcgttcaag attaaaacgg tgactataca aaaagttttc gtttttagtt gcattactta
    75361 aacgcataag gtctttctgc ccgttgtgat aaaagctatg cttaaccaat cttcctaaat
    75421 tttgagttga gccccattca ttcgtagcat ttttaaaatc actattctta atgaggttag
    75481 ggccgcttac actatatttc ccaacctcaa cctgaaacag ttgattagtc agagccatgc
    75541 gagcaacctt atccgcaatt ccattttcag tattgcccaa aattcgctcg taaagtttac
    75601 tggtttcctt aacacgctgg aagtcagtag tctctacttt tcgcgctagt tgattggtca
    75661 cattcgcaaa ttgactatca gcattcgctt tgtttgcaga aacctgatca gatattctac
    75721 ccatttgtcg ttcagcatta tccttgtttg tagcgacctg agtctttaaa tttgaaatct
    75781 gattatctgt gccttgctta ttactgttta tccgatttga aatatttgaa atctgagtag
    75841 tggttccttg ctcactgctt gtaagtctat ttgatagacc actgatttga ccgcccacat
    75901 cttgcttata agtagttatc tgacttgaaa tatccgtgaa cttaccatct acagattgac
    75961 gatagctagc gatttgacta gcgatgtctt tattcgcact agttttaaca gcttcaatcc
    76021 tctgattgat acccttaaca tcttcttgat aagtagcctt accaacgaaa tcacgattga
    76081 ccagctcacg gactgctgtc gcttgtctcg tgctttcctc acgagtatag cgctgtaggg
    76141 cttcctgtcg ctgaccgtct tgaccaacat agctctcaac tgctgccatc ttagtagata
    76201 ggccatcagc tgtcctcttg aactcggttt tagcgactaa aagctcattt tctccgtctt
    76261 ctggtgctgg tgaccagtcc gtcgccacac taccgatttc aaccttgatt cctgttaccc
    76321 aagctgtacc gcttgtagca ccttcaagat tgaatcgcaa tgatgtcttc aattgatcaa
    76381 aatttgtttt ttcagagtag tcataagtga atgtaatata tttccaatct gccgaacctt
    76441 tatacatacc aagcgtagca taatctggac cactctgtac tccggtctca ctattttttc
    76501 taaaaaga
//
LOCUS       DAVKQB010000002        68947 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKQB010000002 DAVKQB010000000
VERSION     DAVKQB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 68947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.002"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..171
                     /locus_tag="VW528_000077"
     CDS             <1..171
                     /locus_tag="VW528_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9910975.1"
                     /translation="VKDKGLWYYLNESGSMATGWFTVSGQWYYTYNSGDLLVNTTTPD
                     GYRVNANGEWVG"
     gene            complement(650..799)
                     /gene="rpmG"
                     /locus_tag="VW528_000078"
     CDS             complement(650..799)
                     /gene="rpmG"
                     /locus_tag="VW528_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9910976.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(815..997)
                     /gene="rpmF"
                     /locus_tag="VW528_000079"
     CDS             complement(815..997)
                     /gene="rpmF"
                     /locus_tag="VW528_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9910977.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            complement(1149..1349)
                     /locus_tag="VW528_000080"
     CDS             complement(1149..1349)
                     /locus_tag="VW528_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9910978.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            complement(1352..2359)
                     /locus_tag="VW528_000081"
     CDS             complement(1352..2359)
                     /locus_tag="VW528_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9910979.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            2550..4580
                     /locus_tag="VW528_000082"
     CDS             2550..4580
                     /locus_tag="VW528_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9910980.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            4584..4868
                     /locus_tag="VW528_000083"
     CDS             4584..4868
                     /locus_tag="VW528_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9910981.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            4881..6227
                     /locus_tag="VW528_000084"
     CDS             4881..6227
                     /locus_tag="VW528_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9910982.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFITKPSAKEE"
     gene            6231..7088
                     /locus_tag="VW528_000085"
     CDS             6231..7088
                     /locus_tag="VW528_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9910983.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            7085..8017
                     /locus_tag="VW528_000086"
     CDS             7085..8017
                     /locus_tag="VW528_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9910984.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            8250..9953
                     /gene="ilvD"
                     /locus_tag="VW528_000087"
     CDS             8250..9953
                     /gene="ilvD"
                     /locus_tag="VW528_000087"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9910985.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(10114..10194)
                     /locus_tag="VW528_000088"
                     /pseudo
     CDS             complement(10114..10194)
                     /locus_tag="VW528_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10252..10593)
                     /locus_tag="VW528_000089"
     CDS             complement(10252..10593)
                     /locus_tag="VW528_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9910986.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            10717..11568
                     /gene="rgg"
                     /locus_tag="VW528_000090"
     CDS             10717..11568
                     /gene="rgg"
                     /locus_tag="VW528_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9910987.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            11761..13008
                     /locus_tag="VW528_000091"
     CDS             11761..13008
                     /locus_tag="VW528_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9910988.1"
                     /translation="MKLLFRNPAYRILILSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            13086..14375
                     /locus_tag="VW528_000092"
     CDS             13086..14375
                     /locus_tag="VW528_000092"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9910989.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKEA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVSYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            14366..14446
                     /locus_tag="VW528_000093"
                     /pseudo
     CDS             14366..14446
                     /locus_tag="VW528_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            14716..15030
                     /locus_tag="VW528_000094"
     CDS             14716..15030
                     /locus_tag="VW528_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9910990.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            15040..15246
                     /locus_tag="VW528_000095"
     CDS             15040..15246
                     /locus_tag="VW528_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9910991.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            15246..15443
                     /locus_tag="VW528_000096"
     CDS             15246..15443
                     /locus_tag="VW528_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9910992.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQGDQFVWLYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            15446..16180
                     /locus_tag="VW528_000097"
     CDS             15446..16180
                     /locus_tag="VW528_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9910993.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            16192..16833
                     /locus_tag="VW528_000098"
     CDS             16192..16833
                     /locus_tag="VW528_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9910994.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            16851..17081
                     /locus_tag="VW528_000099"
     CDS             16851..17081
                     /locus_tag="VW528_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9910995.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            17433..19196
                     /gene="aspS"
                     /locus_tag="VW528_000100"
     CDS             17433..19196
                     /gene="aspS"
                     /locus_tag="VW528_000100"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9910996.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            19174..20115
                     /locus_tag="VW528_000101"
     CDS             19174..20115
                     /locus_tag="VW528_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9910997.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(20325..20405)
                     /locus_tag="VW528_000102"
                     /pseudo
     CDS             complement(20325..20405)
                     /locus_tag="VW528_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(20541..21527)
                     /locus_tag="VW528_000103"
     CDS             complement(20541..21527)
                     /locus_tag="VW528_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9910998.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(21537..22337)
                     /locus_tag="VW528_000104"
     CDS             complement(21537..22337)
                     /locus_tag="VW528_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9910999.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PGQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(22598..23440)
                     /locus_tag="VW528_000105"
     CDS             complement(22598..23440)
                     /locus_tag="VW528_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9911000.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(23442..24734)
                     /locus_tag="VW528_000106"
     CDS             complement(23442..24734)
                     /locus_tag="VW528_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9911001.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(24845..26116)
                     /locus_tag="VW528_000107"
     CDS             complement(24845..26116)
                     /locus_tag="VW528_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9911002.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            26666..28183
                     /gene="malQ"
                     /locus_tag="VW528_000108"
     CDS             26666..28183
                     /gene="malQ"
                     /locus_tag="VW528_000108"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9911003.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            28209..30467
                     /gene="glgP"
                     /locus_tag="VW528_000109"
     CDS             28209..30467
                     /gene="glgP"
                     /locus_tag="VW528_000109"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU9911004.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(31512..32084)
                     /locus_tag="VW528_000110"
     CDS             complement(31512..32084)
                     /locus_tag="VW528_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911005.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTTIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(32707..33555)
                     /locus_tag="VW528_000111"
     CDS             complement(32707..33555)
                     /locus_tag="VW528_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9911006.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            33707..33985
                     /locus_tag="VW528_000112"
     CDS             33707..33985
                     /locus_tag="VW528_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911007.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            33987..36050
                     /locus_tag="VW528_000113"
     CDS             33987..36050
                     /locus_tag="VW528_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9911008.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIKLMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(36128..37384)
                     /locus_tag="VW528_000114"
     CDS             complement(36128..37384)
                     /locus_tag="VW528_000114"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9911009.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            37526..39991
                     /locus_tag="VW528_000115"
     CDS             37526..39991
                     /locus_tag="VW528_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9911010.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            40056..40967
                     /locus_tag="VW528_000116"
     CDS             40056..40967
                     /locus_tag="VW528_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9911011.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            41197..41895
                     /gene="dapD"
                     /locus_tag="VW528_000117"
     CDS             41197..41895
                     /gene="dapD"
                     /locus_tag="VW528_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9911012.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            41963..43093
                     /locus_tag="VW528_000118"
     CDS             41963..43093
                     /locus_tag="VW528_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9911013.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            43105..43644
                     /locus_tag="VW528_000119"
     CDS             43105..43644
                     /locus_tag="VW528_000119"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9911014.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVVYDPQQLVKTSFALLEPQ
                     GDVEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            43628..44305
                     /locus_tag="VW528_000120"
     CDS             43628..44305
                     /locus_tag="VW528_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9911015.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(44377..45276)
                     /gene="galU"
                     /locus_tag="VW528_000121"
     CDS             complement(44377..45276)
                     /gene="galU"
                     /locus_tag="VW528_000121"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9911016.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(45298..46314)
                     /locus_tag="VW528_000122"
     CDS             complement(45298..46314)
                     /locus_tag="VW528_000122"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9911017.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            46536..46939
                     /locus_tag="VW528_000123"
                     /pseudo
     CDS             46536..46939
                     /locus_tag="VW528_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(47054..47479)
                     /locus_tag="VW528_000124"
     CDS             complement(47054..47479)
                     /locus_tag="VW528_000124"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9911018.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(47547..48197)
                     /gene="phoU"
                     /locus_tag="VW528_000125"
     CDS             complement(47547..48197)
                     /gene="phoU"
                     /locus_tag="VW528_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9911019.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(48212..48964)
                     /gene="pstB"
                     /locus_tag="VW528_000126"
     CDS             complement(48212..48964)
                     /gene="pstB"
                     /locus_tag="VW528_000126"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9911020.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(48966..49781)
                     /gene="pstA"
                     /locus_tag="VW528_000127"
     CDS             complement(48966..49781)
                     /gene="pstA"
                     /locus_tag="VW528_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9911021.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(49774..50637)
                     /gene="pstC"
                     /locus_tag="VW528_000128"
     CDS             complement(49774..50637)
                     /gene="pstC"
                     /locus_tag="VW528_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9911022.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(50755..51630)
                     /locus_tag="VW528_000129"
     CDS             complement(50755..51630)
                     /locus_tag="VW528_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU9911023.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGKYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(51797..53128)
                     /locus_tag="VW528_000130"
     CDS             complement(51797..53128)
                     /locus_tag="VW528_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9911024.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMVLTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(53129..53836)
                     /locus_tag="VW528_000131"
     CDS             complement(53129..53836)
                     /locus_tag="VW528_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9911025.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(53920..54222)
                     /locus_tag="VW528_000132"
     CDS             complement(53920..54222)
                     /locus_tag="VW528_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9911026.1"
                     /translation="MTADEIWYFHAESPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            complement(54209..54430)
                     /locus_tag="VW528_000133"
     CDS             complement(54209..54430)
                     /locus_tag="VW528_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9911027.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            54392..54950
                     /locus_tag="VW528_000134"
                     /pseudo
     CDS             54392..54950
                     /locus_tag="VW528_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55265..56956)
                     /locus_tag="VW528_000135"
     CDS             complement(55265..56956)
                     /locus_tag="VW528_000135"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9911028.1"
                     /translation="MNTKELIASELSSIIDSLDQEAVLKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            57092..57538
                     /gene="argR"
                     /locus_tag="VW528_000136"
     CDS             57092..57538
                     /gene="argR"
                     /locus_tag="VW528_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9911029.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            57589..60123
                     /gene="mutS"
                     /locus_tag="VW528_000137"
     CDS             57589..60123
                     /gene="mutS"
                     /locus_tag="VW528_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9911030.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDCEGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            60270..61964
                     /gene="patA"
                     /locus_tag="VW528_000138"
     CDS             60270..61964
                     /gene="patA"
                     /locus_tag="VW528_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9911031.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELFGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(62295..62564)
                     /locus_tag="VW528_000139"
                     /pseudo
     CDS             complement(62295..62564)
                     /locus_tag="VW528_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            62756..64522
                     /gene="patB"
                     /locus_tag="VW528_000140"
     CDS             62756..64522
                     /gene="patB"
                     /locus_tag="VW528_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9911032.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAHQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPIMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            64661..65350
                     /locus_tag="VW528_000141"
     CDS             64661..65350
                     /locus_tag="VW528_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9911033.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            65354..65533
                     /locus_tag="VW528_000142"
     CDS             65354..65533
                     /locus_tag="VW528_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911034.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            65520..66869
                     /locus_tag="VW528_000143"
     CDS             65520..66869
                     /locus_tag="VW528_000143"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9911035.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            66993..68453
                     /locus_tag="VW528_000144"
     CDS             66993..68453
                     /locus_tag="VW528_000144"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9911036.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            68467..68901
                     /locus_tag="VW528_000145"
     CDS             68467..68901
                     /locus_tag="VW528_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9911037.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT        20221 a        12824 c        14910 g        20992 t
ORIGIN      
        1 gttaaagaca aaggcttatg gtattactta aatgaatcag gttcaatggc tactggttgg
       61 tttacagttt ctggtcaatg gtactatacc tataattcag gagatttatt agtaaacacg
      121 actacacccg atggctatcg agtcaatgct aacggtgagt gggtaggata ggtatccatt
      181 agctaaatct aatcattctg aactaaaaaa tcttcttaca tatgtaggaa gattttttgg
      241 attctcaaaa ctagtttaaa agactacgct atttaaacca attttaagaa tggatatata
      301 aggatattca gaaatctagt tggtcaactt ttgtattttg aatatacctt agatggtgac
      361 gatgtcttgg tatatctggt actcacttac atattctaac ttattttaac taagatacaa
      421 ctgtagttgg tgaatttgct acaaatagtg tagaggcgtt atttgaaaaa tatacaaaaa
      481 aggaacccca acttacattt gagttcctct tgcttttaat tgtccgaatc cgtattctta
      541 tttcaataat gtagtcattc gaatgatttc ccttgcaata tagtgaaatt aacaaaaaga
      601 aaaaacgctg atcaagcgtt tttcttctgt attgattcgt attgaatgct tacttcactt
      661 ctgtaaacac aacgtgtttg cgaagttttg gtgagtattt cttcaattga aggcggtctg
      721 gagtgttacg tttgttttta gaagtaaggt acaagcgttc accagattct ttgtgttcaa
      781 gtgtaatatt tacgcgcata gtatctccct tctattattc agctgatgca gctttagcga
      841 ttttacgtcc tttgtagtat cctttaagtg atacacggtg agaacgtgag taatctccag
      901 tagtttcgtc aaagtttaca gatggagctg ttactttgta gtgtgtacga cgtttgtttt
      961 tcttcgcttt tgaagtgcga cgtgcaggta ctgccatttt gatttctcct ttaggtattt
     1021 atattcgatt caatctactg attttcatca accatactag gataacacat tttttttgta
     1081 aagtaaagtt acttgacaaa aaaagatgaa aaaattctcc tatccgacac ggcagacagg
     1141 agaaggtgtt aaatatcaat ctcaaatggt tcgtcaatgg tttctgatac gtattttccg
     1201 tcttttttcc gttgcttgac acactctgtg aggagatatt ctatttgccc attgactgaa
     1261 cgaaagtcgt cttctgccca tgatgcgagt gcagcgtata actttgttga gagtcgaagg
     1321 gggatctgct tttttttagc ttcagccatc tttagtaaag acttcctgtg ttgacaattg
     1381 gttgtgcatc atgattgcca caaagaacga caaggagatt tgaaaccatg gcagcttttc
     1441 gttcttcgtc aagttctacc aattcccctt cattgagccg ttctagtgcc atttcaacca
     1501 ttcctacagc accatctaca atcatcttac gtgcatcaat aatggcagat gcttgttggc
     1561 gttgaagcat aacggcagca atttctggag cataagctag gtaagtgata cgtgcttcaa
     1621 ggatttccaa gccagcatcc tcaacacgac tttggatttc ttcacgaata cggttagcaa
     1681 caatttcgct agagccacgg agactacctt catctgcttg cccgtcaccc gtagtatcca
     1741 cattaggaga cacatcgtaa ggatagatgc ggacaatatt acggagggcg ctatcacatt
     1801 gcaatgaaag atattctttg tagttatcaa cgttgaagac tgccttagcg gtatcgacaa
     1861 ctctccaagt taccgcgata ccgatttcta cagggtttcc taagcaatca ttgatttttt
     1921 gacgagaatt gctcaaggtc atgactttga gggaaatctg tttcttgcca atttcaagat
     1981 ttacatcatt gtcatttgat gatttagctc ctaaaaaagg agattttgtg ctaacatcac
     2041 cactttgtcc aagtcgagtg tggtttgcag ggttgactgc tacgctgaag ggattgacaa
     2101 agtaaaaacc aggttctttg atggtacctg tataattacc aaagagtgtc agaaccagag
     2161 cttcctgagg tttgacaact tttaaaccag catgagctag acctgcaatt acgattagta
     2221 aaggtccgat aataattcca aaaatgtttt ccgaaccaac acccattata aatataaaga
     2281 taccaagtac gattgtcaat tcaatgagaa tcaatgcgaa tacaccattt ggttttgaat
     2341 tgatgagttt ttcagtcata agaatgacct cctattattt gatatctaaa tgatatcact
     2401 ttgtttttaa aagtcaatag aaagttgaaa ataatcacta aaaagtttca gcccaataat
     2461 ttcttttaaa aggtgagatt atattgtgct caaatactct tttattctta taatttttac
     2521 actataattg tagttagaaa ggaagagaaa tgtttgatta tagagcacta gttattttga
     2581 tgactttgat ggatcattgt gagctatcat tatatgaatt atctgttaag gtgagcttac
     2641 ctataaagga agtcaaagaa ggaatagatt atttagtgcc ttatctagct aataaaggga
     2701 tagtgctgga taaaaaacaa ggtcgctata gtttatcaaa tcgtacgaag cagtctttga
     2761 cagatattat taagtcggat gaattggttt taccaaagtc gactcgctta gctttaatct
     2821 atctttacac tttttgccga ttagatttta tatcgaataa tcattaccaa gactttttga
     2881 aagtaagtaa gaatacaacc ttatctgata ttcaatcatt aagaaagatt atgttagata
     2941 atgatttgga gctagggtac agtagagcaa aaggttatac tttacacggt tcagagtgga
     3001 ataagcaccg cttagctttt caaatggtta gtgagttgct ggaatcctcc atagggattt
     3061 ggggtttgga ttatgtttta tccagttggg ggtattcatt aacttatgat ttgataaatc
     3121 aggtagttaa agattattat gagaagctac agctagtacc tattgttaat caattaaaag
     3181 tttgcctttt cggtttagta ttcattctgt gtaggtatca aagggatgtt gaaagagtat
     3241 gtctttcaga gacattagta tctcctgtta ttcaagatat aacgactatt ttattggata
     3301 cagtggttga tttgggaatt atagatacgg tgttttctga ggatgattat cgctatatca
     3361 ccgttttatt atcaagttgc tttgaaggtg aagtagatgt tgctccggtt tactttaatc
     3421 aattaacaga ggctattata agtagaatgg aagatatttc tttgttacat tttaaacaaa
     3481 gggaaggatt gagagaaaat cttcgccgcc accttatacc ggcttactat agattaaagt
     3541 tcggcctacc tagttcaaat gaatatgtat tgcatgttaa agaacattat cctgatttat
     3601 ttgaattagt caaagattct ttgatgccct tgatggacgc tattgacaat cccatacctg
     3661 atagtgaaac agcatatttt gttatccatt tcggaggtta tttaaagaaa gcagatactt
     3721 tgcctcaaaa atgctataaa gcagcaatca tttgtcctaa tggtgttagt tcttcattga
     3781 tgttaaaaga gaatctatta gcattatttc ctcaaattga gtttatagga acctcaaaga
     3841 ttgatgattt acaggtgaaa gctagtagtg actatgatat ggttttttct accataaagg
     3901 tggagacaga aaagccaaat tatctagttt cggttatgat gactgaagag caagcaatac
     3961 aactagtaga actagtgtta aaagattttc cgaatttaga gtatggggat tttgagattg
     4021 aacaaatcct gaatattgtc aaaagatatg gaattattac acaagaattg gaattaagat
     4081 tggcgttaaa gaattatctt taccaaaaaa atgatagaaa ggaaattgta ccagtgctag
     4141 aacaactcat taccaaagaa acctatcagg ttagttcgca aaaattagga tggaaggagg
     4201 cgattcgttt agcagctaaa ccgcttttgg atcaagataa gataactgag aactaccctg
     4261 aggcaatgat tcaaaaagta gaagagtttg ggccttttat taatttaggg aagggagtag
     4321 caattcctca cgctcggcca gatgaaggtg tgaatgaaat aggaatgtca atgttagttt
     4381 tggaagaacc gatttattta ttggataatc cagaacaaga ggtaagattg ttgatttgta
     4441 ttgcagccat tgataatgag agtcatttaa aggctttgtc acatttaaca acaattttaa
     4501 gagataaaaa tcatgttcaa actttaatat catcaaaaaa ctatgatgac attaaaatga
     4561 taattaaaca ggaggattag ataatgttaa aaattggtac agcttgtggt tcaggattag
     4621 gttcaagttt tatggtacag atgaatattg aatctgtatt gagtgatttg aatgtttcgg
     4681 atgtagaagt tgaacattat gatttaggtg gagcagatcc aaatgcagct gatatttgga
     4741 ttgttggtcg tgatctagct gattcagcta gtcatcttgg agatgttcgt atcttaaata
     4801 gtattattga tatggatgaa ctacgagaat taattactaa actttgtgaa gaaaaaggac
     4861 ttatatagga ggctagcgtt atgatgaagt tcatattgga tattgttagt acaccagcta
     4921 ttttagtagc tttaattgca attttaggat tagttcttca gaagaagaaa ttacctgata
     4981 ttattaaagg tggaattaag acctttgttg gtttcttagt tgtatctggt ggtgcaggaa
     5041 ttgtacaaaa ttctttaaat ccatttggta ccatgtttga gcatgctttt catttatctg
     5101 gcgttgtgcc gaataatgaa gcaattgtag ctgtagcttt aacaacatat ggctcagcta
     5161 ctgcactgat tatgtttgca ggcatggtgt tcaatatctt aatcgctcgt tttactcgat
     5221 ttaaatatat ttttttaaca gggcaccaca ctctatatat ggcatgtatg attgcggtca
     5281 ttttatcagt tgctggcttt actagcttgc ctcttatctt actaggagga ttagcactcg
     5341 gtattattat gagtatttcc ccagcatttg ttcaaaaata tatggttcaa ttaactggaa
     5401 atgacaaggt ggctttaggt catttcagtt ctttgggata ttggttgagt ggttttactg
     5461 gtagccttat cggtgacaaa tcaaaatcaa cagaggacat taaatttcca aagagtttag
     5521 cttttttacg tgatagtact gttagtatca ctttatccat ggcagttatt tacattattg
     5581 tagctatctt tgcagggtca gaatatatag aaaaagaaat cagtagtggt acaagtggtc
     5641 tagtttatgc tttacaatta gcaggtcaat ttgcagcagg ggtatttgtt attttagcag
     5701 gtgttcgcct tattttgggc gaaattgttc cagcctttaa aggtatttca gagcgtcttg
     5761 tacctaattc aaaacctgct ttggattgtc cgattgttta tacttatgca cccaatgcag
     5821 ttctaattgg atttatctct agttttgttg gtggtttagt aagtatggca attatgattg
     5881 cttcaggaac ggttgttatc ttaccaggtg ttgtgcctca tttcttctgt ggagcgactg
     5941 caggtgtcat tgggaatgca tctggtggtg ttcgtggagc cactattgga gcatttttac
     6001 aaggtatttt aatcagtttt cttccagtct ttttaatgcc agttttggga ggacttggtt
     6061 tccaaggatc aactttctca gatgcagatt ttggtctatc aggaattatt ttaggaatgt
     6121 taaatcaatt tggctcacaa gcaggcattg tgattggtct tgttcttatt ctagcagtta
     6181 tgtttggagt atcctttatt acaaagccat ctgcaaagga ggaataaggg atgattttaa
     6241 gtaaaaatag ggaagatgag ttaagaaaat ttgcgactaa catccgatta aatactctta
     6301 gaacattgaa tcatcttgga ttcggacatt acggaggaag tctgtctatc gtagaagttt
     6361 tagcagtgct ttatggtgaa ataatgccaa tgactccaga aatatttgca gcacgagata
     6421 gagattattt catattatca aaaggtcacg gaggaccagc tctatacagt acactctatt
     6481 tgaatggttt ctttgacaaa gaattcttat attctttaaa tacaaatgga accaaattac
     6541 cgtctcatcc tgatagaaat ctaacgccag gcatagatat gacaacgggc tctttaggac
     6601 aaggaattag tgttgcaact ggacttgcat atggtcagag aataagaaag agtccctttt
     6661 atacttatgc tattgttgga gatggtgagt taaatgaggg acaatgttgg gaggctatac
     6721 agtttgcttc tcatcaacag ttatccaact taattgtatt tgttgatgat aacaaaaaac
     6781 aattagatgg ttttacaaag gatatttgta atccaggtga tttcgtagaa aaattttcag
     6841 catttggatt tgaatccatt agggtcaagg gttcagatat tagagaaatt tatgaaggga
     6901 ttgtccaatt aaaacagtca aataattcat cacctaagtg cattgtatta gatactatta
     6961 aaggtcaagg ggttcaagag ctggaagaaa tgaaatccaa ccatcatctt cgccctactg
     7021 tagaggagag acaaatgtta acttcagttg tagaaagatt aagtcaggaa ttggaggaaa
     7081 cagaatgatg agaagcacga aagaattacg gcatgtatat agagacttcc ttttagaggc
     7141 taatcaaagt tattctgata tagtagtctt agaagccgat ttgtcaagtt cgatggctac
     7201 taataatctt gaaaaggatt ttggagaccg ttatgtgaat gttgggatta tggaagcaga
     7261 aatggtcggc cttgcagcag gcttgtctat tcaggggttt agaccttatc ttcatacatt
     7321 tggtcctttt gcttcacgaa gagtatttga tcaattattt atttctcttg gatacgcaca
     7381 attggatgcc actgtgattg gatcagatgc aggagtaact gcagaaatga atggtgggac
     7441 acatatgcca tttgaagaaa ttggattgtt acgtttaatt cctaaatcga tcatttttga
     7501 agcaactgat gatatccaat ttcgtgaaat attgaaccag acattagact taaaaggact
     7561 aaaatatatt cgaacaatta gaaaagctcc agttcctgtg tatcaaggtg gagaagattt
     7621 ttctaagggc tacattgagt taaggcatgg tgaagatctt gtaatcgttg cttctggtat
     7681 aatggttgct ccaagtattc gagttgcaga tgaactgtct aaattaggtt attcagtaag
     7741 tgtgatagat ttatttagaa tcaaaccgat accagaacag ataaaaacaa tgttaagtgg
     7801 aaaaactata tttactgtag aaaaccacaa tcagataggt ggaattggca gttctttatg
     7861 tgaattattc tctgatgatg gaatgacaaa aattcatcgg atgggtgttc aagaaagatt
     7921 cggtcaagta gggaaaatgg attatcttct taatgagtat ggtttgagtg aatcgaatat
     7981 aaaagagaag gttctagaaa tttataatgc aagatagatg ttaaatacac ttatctgaaa
     8041 gaatttctgt aaaaattaca gtacgttatt taaagaaagc ggattaaaaa gcatattctg
     8101 gtcatcagaa agtgcactaa tccgctttgt tgtttttaat tttaaatatt tcacatgtgt
     8161 atagaatttt ctgaaaattc aagattttta ttctgttctt catgcgaaaa ttgtgctata
     8221 atagtaaaaa ctgaaatggg agggataaga tgactgaatt agataaacgt caccgcagta
     8281 gcatttatga cagcatggtt aaatcaccta accgtgctat gcttcgtgcg actggtatga
     8341 cagataagga ctttgaaaca ccgattgtgg gagtgatttc gacttgggcg gaaaatacac
     8401 catgtaacat tcacttgcat gatttcggga aactggctaa agaaggtgtc aaatctgcag
     8461 gcgcttggcc tgtacagttt ggaaccatta ccgtagcaga cgggatcgct atgggaacgc
     8521 ctggtatgcg tttctctcta acatctcgtg acatcatcgc ggactccatc gaggcggcta
     8581 tgagtggtca caacgtggat gcctttgtcg ctatcggtgg ctgtgacaag aacatgcctg
     8641 gatctatgat tgctattgct aatatggata tcccagctat tttcgcctat ggtggaacta
     8701 ttgcaccggg aaatcttgat ggtaaagata tcgacttggt ttctgtcttt gaaggtatcg
     8761 gaaaatggaa ccacggtgac atgacagctg aggacgtgaa acgtcttgaa tgtaatgcct
     8821 gccctggccc tggtggctgt ggtggtatgt atactgctaa taccatggca actgctatcg
     8881 aagttctagg gatgagtttg ccagggtcat cctctcaccc agctgaatca gctgataaga
     8941 aagaagatat cgaagcagca ggacgtgctg ttgttaagat gttggaactt ggtctcaaac
     9001 catcagatat cttgactcgt gaagcctttg aagatgctat tactgtaacg atggctctcg
     9061 gtggttctac aaacgccact cttcacttgc tcgccattgc ccatgccgca aatgttgact
     9121 tgtcacttga ggacttcaat acgattcaag aacgtgtgcc tcacttggcc gacttgaaac
     9181 catctggtca gtatgtcttc caagacctct acgaagtcgg tggtgtccct gcggttatga
     9241 agtacttgtt ggcaaatggt ttccttcacg gagatcgcat cacatgtact ggtaagactg
     9301 tagctgaaaa cttggctgac tttgcagact tgactccagg ccaaaaagtt atcatgccac
     9361 ttgaaaatcc aaaacgtgcg gatggtccgc ttatcatctt gaacgggaac cttgctcctg
     9421 acggtgcagt tgccaaggta tcaggtgtta aagtgcgtcg tcacgttggg ccagctaagg
     9481 tctttgactc agaagaagat gcgattcagg ccgttctgac agatgaaatc gttgatggcg
     9541 atgtagtcgt tgttcgtttt gttggaccta aaggtggtcc tggtatgcct gagatgctgt
     9601 cactttcttc aatgattgtt ggtaaaggtc agggagataa ggtggccctc ttgacggacg
     9661 gacgtttctc tggtggtact tatggtctgg ttgttggaca tatcgctcct gaagctcagg
     9721 atggtggacc aattgcctat ctccgtaccg gcgatatcgt tacggttgac caagatacca
     9781 aagaaatttc catggccgta tccgaagaag aacttgaaaa acgcaaggca gaaacaacct
     9841 tgccaccact ttacagccgt ggtgtcctcg gtaaatatgc ccacatcgta tcatctgctt
     9901 cacgcggagc cgtgacagac ttctggaata tggacaagtc aggtaaaaaa taaactcata
     9961 ctcgaaaatc tcttcaaatc acgtcaacgt cgccttgccg taggtatggt tactgacttc
    10021 gtcagttcta tccacaacct caaaacagtg ttttgggctg acttcgtcag ttctatccac
    10081 aacctcaaaa cagtgttttg agctgacttc gtcagttcta tccataactt caaaacagtg
    10141 ttttgagcaa cctgcggcta gttttctagt ttgccctttg attttcattg agtatcaaaa
    10201 agcaagccat tttcggcttg cttttttcat ctttaaaagt gattggcctg cttaacgagg
    10261 tggtagtcca agggcaatac ggccatagcg actgattcgt gtgattttcc aagcaggcga
    10321 ccaggtaact tcaaccttga tatctttgat accctcgatt tgtttcagac ctgccacaat
    10381 ttcaataggc aggctttcgg cgcaatcaca ggcagtatcg gtgaaggtca tgacaatctt
    10441 gcagagcccc gtttcatcca gattgatttc ataaatcaga cctagattgt aaacatccaa
    10501 ttccacatct gtatcaaaaa ctttctctag tttttcgata atttggtctt gcaaggccaa
    10561 agcacggtca ttgattttga tatcgtctct catagcagtc cctccacgtg ataaatatcc
    10621 tctattttct cataattctg acaatttggc aagtttttga tgaattttct gaaaaatagg
    10681 ataaaatgaa gaaaatacgg aatcaagggg aagtctatgg agcagattgg aaaagtcttt
    10741 agacaattac gagagtcaag aaatatctca ctgagacaag caaccggagg acaattttcg
    10801 ccgtctatgt tgtcccgttt tgaaacaggt cagagtgagc tttcggtgga aaagtttcta
    10861 tttgctctgg aaaatatatc tgccagtgtg gaggagatcc tctttctggc gagaggtttc
    10921 cagtatgata cagattctga gttgagaaaa gaaattacag atgtcttgga accaaagaat
    10981 atagcacctc ttgaggactt atatcgcagg gagtatcaaa agcatgccca ttctcacaac
    11041 aaacagaaac atattctaaa tgccattatt atcaagtctt atatgaagag catggatgaa
    11101 agggtagagt taacagcaga ggaaggaaaa gtccttcatg attatttgtt ttctaccgag
    11161 atctggggaa tctatgaact caatttattt tcagtcagtt ctccattttt atctgtttct
    11221 ctttttacta gatatgtacg agaaatggta cgtaaatctg attttctaat ggaaatgtct
    11281 ggcaatcgaa acctttttca tactatacta ttgaatggct ttttagctag tattgagtgt
    11341 gaagaattta ccaatgccta ttattttaaa cgtgttatcg aagagcattt ctacaaggaa
    11401 aatgagacct atttccgaat tgtttatctg tgggcagaag gtctccttga tagcaagcaa
    11461 ggtagagtca aggaaggcca gaaaaagatg gaggatgctg tccgtatttt tgagatgctt
    11521 ggctgtaata aatctgccga atactataga aatacgaccg aatgttgaat atctgcaaac
    11581 caagaaaaaa tttgaagcga tagaactatt gccctctctc aggtcattac aaaagttcta
    11641 tcgtttcttt ttgctcattt tatttgttgc atatattcaa gagttctccc tctcaaattt
    11701 ctaagtgcta tactatagtc atacttcata tagaggttcg aacaagaagg gagattatct
    11761 atgaaactat tgtttagaaa tccagcttat agaattttga ttttatcacg cttcttcaat
    11821 gcttttggtg cttcgatttt caacctggtc tttatcgtct atgcatcgac cttgtcacaa
    11881 gcatcttttg ctgttgctat ggcgaatatt gtcatgattc ttccgaccct ctttacagtt
    11941 tttgtaggga ttcgggcaga ttacacgagg gacaaggtca aatggatggt ctatagcggt
    12001 ttgtttcagg cggttctatt ttttctagca gccctagttg ttcagcaagc tagtctcttt
    12061 gccttttcta gcctgtgttt gatcaatgtc attagtgata tcatcagtga ttttgcaggt
    12121 ggcctgcgta tgcctcttat taaggaaaaa gtagctgaag atgatttgat ggaggcttat
    12181 tctttttcac agttcatcac ctatatttca gctattggtg gtcaagcttt cggagtctgg
    12241 ctcttagctc tatcggtcaa caatttttct ctcgttgcgg gaatcaatgc ctgctttttc
    12301 ctagtatcag ccactatcct ctttttagga aaaagcaaat tgagcctgtc aatgtcatct
    12361 gctgatggtg aaaacttaaa aaatgagaag ctttctatca aagaccagtt cctaacaatt
    12421 taccgaaatt tacgtctcgt ttttcttaaa agtgggcaga aaaactttgg ttttatgctc
    12481 tttgctgtct tgcttattaa ttccttgggt ggcgctttgg gtggaattta taatatcttc
    12541 tttttgagcc attctctctt gaatttttct tatacagagg cactatttat caatcaattc
    12601 tgtgttttag tagcaatcat catcagtagc cttacgggca atgattattt tgggaagcag
    12661 tccttgccta gattgatgat gtgggcgacc gtaggactta gtctagttgg tttggctaat
    12721 ttattcaatc aagtcgtgct tggtttgcta tttctcttct ttaccctgta tgtgtctggt
    12781 aaagttcaac caaagattag tgccatgctc atgaaaaatc tagctccaga ggttctagct
    12841 cgtactagta attttttagg tctattgttt accttatcca tacctgtggg aacagcttgt
    12901 ttttcactag tagccgtatg gaatatacag ttgacttgga tgctatttgt tggtctttcc
    12961 ttgctagcta tttttttgac aattcttaat ctcaaaaatg atatctaaat cctaattttt
    13021 ttcttactgt tttaatccct ttatttgtgg taaaataaga ctattaagtt taaagaggat
    13081 tccctatgaa attacaaaaa ccaaaaggaa cgcaggatat tttacctgct gaatctgcta
    13141 agtggcagta cgttgagggc tttgcccgtg agattttcaa gcgctataac tatgcagaag
    13201 tgcgcacgcc tatttttgag cattacgagg ttatcagtcg ctctgtcgga gatacaacgg
    13261 atatcgtaac caaggaaatg tacgattttt atgacaaggg tgaccgtcat attaccctcc
    13321 gtccagaagg aactgcgccc gttgtccgtt cctatgtgga aaataaactc ttcgccccag
    13381 aagtgcaaaa gccaagcaag ttctactata tgggacctat gttccgttat gagcgtccac
    13441 aggcagggcg cttgcgccaa ttccaccaaa ttggtgttga gtgttttggc tctagcaatc
    13501 cagctaccga tgtggaaaca atcgctatgg cagcccattt tttgaaggaa atcggtattc
    13561 aaggtgtcaa attgcacctc aacactcttg gaaatcctga gagccgtgca gcctaccgcc
    13621 aagccttgat tgactatttg acaccgctca aggagacctt gtctaaggat agccaacgtc
    13681 gcttggagga aaatcctctt cgtgtcttgg actctaagga aaaagaagac aaggaggcag
    13741 tagagaatgc gccgtctatc ttggactttc ttgatgaaga aagccaagct cattttgatg
    13801 ctgtgcgtca gatgttggaa aatcttggag tagattacat catcgatacc aatatggtgc
    13861 gtggtctgga ctactacaac cacaccattt tcgagtttat cacagagatt gagggcaatg
    13921 acctgaccgt ctgtgcgggt ggtcgttacg atggtttggt ttcttatttt ggaggccctg
    13981 aaactgctgg atttggtttt ggacttggtg tagagcgcct gcttctcatt cttgaaaagc
    14041 aaggtgtgac cctccctatc gaaaacgccc tagatgtcta tatcgcagtc ttgggcgaag
    14101 gggcaaatat caaggccttg gaattggtac aggctcttcg ccaacaaggt ttcaaagcag
    14161 agcgtgatta cctcaaccgt aaactaaaag ctcagttcaa gtcagccgat gtctttgtgg
    14221 ctaagaccct catcacccta ggagagagcg aagtcgaaag cggacaagtg acggtcaaga
    14281 acaaccaaac tcgagaagaa gtgcaagtgt cacttgagac aatcagccaa aacttctcag
    14341 aaatctttga aaaactagga ttttatactc aataaaaatc aaagagcaaa ctaggaagct
    14401 agccgcaggt tgctcaaaac actgttttga ggttgtggat agaactgacg aagtcagctc
    14461 aaaacatggt tttgaggttg tagatgaaac tgacgaagtc agtaaccata cctacggcaa
    14521 ggtgaagctg acgtggtttg aagagatttt cgaagagtat taatgataga taaagtggtc
    14581 aggaacttac tcctgacctt tttcttttgc aaaatgacaa aaatcataaa ctttttgaca
    14641 aatatacttg tcaaaaagaa aaagatatgt tacaatgaat tcaagttaag aaataggaag
    14701 cagaaaggag ttataatgtg ggtactagga tttatactat ttatgatttt cttttattct
    14761 aataattcta aaaaaatcaa gaaactagag aataaaatca aaaaacttga gcgaaaagag
    14821 aaaggaaacg cagaaatgtc gagattatta caagaaatga ttggaaagga accaattata
    14881 acgggagtgt atattgggcc agataactgg gaagttgtgg atgttgatga ggaatgggtc
    14941 aaactacgta gtgttgataa tacgggaaaa gaaaaattca agttgcaacg tattgaagat
    15001 atccaaaccg ttgaatttga cggagagtag gtgtgtaacg tgcaactaaa aaatcgtcta
    15061 aaagagcttc gagctcgcga tggtctcaat caaaccgacc tagccaaact ggcaggggtt
    15121 tccagacaga ccattagcct actagaacgg gatgagtaca ccccatccat tatcattgcc
    15181 ctgaaaatat ctcaaatttt caacgaaaca gtcgaatcgg tatttcgttt ggaggaggac
    15241 gagtgatgaa caagtataaa gtgatttatt atgtagtggt catagcttta ttagtcagcg
    15301 tatttctact gattgggatg gacctaagct ggtttaatcc ctatcaaggc gaccaatttg
    15361 tttggctcta ctttgctctc atcccagtaa ttgaatggat tgaaaagaaa tccaaaaatc
    15421 tagcaagtga aaaaggagaa tgaatatgaa aaagaaacgt agattgttat ttttgatgtc
    15481 tattgtcttg ggggggttct tgggcatgtt tgtagggatg tttaaggcac gtgtcgaatc
    15541 ccacgaaatt attttagatg taaaagcctt gatgccatgg atatcagcta tttgtttact
    15601 gataggtttc attagtatgt ttttgacttt caatttctta aagaaaagca gaaaatttca
    15661 ttccttgtat caagaggaaa tggatgacga tctgaatgaa acctattatg tgcaaatgta
    15721 tcggaatctt gagtttggaa ccattgcttt taatattaca ggtgtagcga ttccattggc
    15781 tatttttatt tcattaagtg aggtgattat attgcataca aaccctcaaa catttttcct
    15841 ttctttctta ctctttgtgg tattcttagt cgctcaaaaa tctcttttta aaaccattgc
    15901 gattgttcgt cagtttgatt tggaattttt cgctacacca aaggatgtct tgaactatat
    15961 aaattcttat gatgaagggg agcgtcaggc taatttggaa cagagttttc gaattttatt
    16021 ccaattacac caatatgtct taccagcctt atatattttt cttattatca tttctttctt
    16081 gacaggagag attcagttac tagctttctt gcttgtagga gccatccatg tttatatcaa
    16141 tgtgatgcag ttacctatgg taaaacgtta tttcaaataa aggagttatc tatgaaatta
    16201 cttaaaaacc ttggctggat tcttctagcc cttctatcct ttttatttat ctatggcttt
    16261 attcaggggc tcgcgactgc gtcgcttgct ttaggcgctt caccctatgc tgttactcta
    16321 ctctatgtgg ccttggctgg agtatatgtg tacggcattt acaaatggta tcagaaggct
    16381 cctgttcata ttgagaagag cggctttaac cgatttattt ggcttcctgc cttggtttgg
    16441 ttcttatctc tggtcgttca attcttcttg ccagatgatc cttcagtaaa tcagcaaata
    16501 gcgacagact tgaccttgtc tcaaccactt ttctcattct ttgctgtggt tatttttgct
    16561 cctttgacgg aagagattgt ttttagaggg atgttagcac gctatctctt tcctaagcag
    16621 gacaatagta aacgaaccct gatttttctt ctggtatcta gtcttctatt tgccttgatt
    16681 cattttccag gtgatgtgca acaatttttt gtctatttta gccttggttt tagtttgggc
    16741 ttggcttaca ttagcagaaa aggtctggtc tacagtattt ctcttcacgc tttgaataat
    16801 ttagtcggct ttttgatgat tctcatgcta taatagagtc aggaggtcac atgaaacgag
    16861 taattttatt agcagtgatt caggcagtcg ttctattttt catcattgga gcgctagctt
    16921 atgccttcaa aggcgatttc ttctataact atctagcagt tgtctttgct cctattgcag
    16981 gtgtactgcg ttttgggacg gcttacataa cggaaattgt cttgcctcga aaggcagccg
    17041 aaatcgctga aaagcgtaaa gcaggcaaaa attcaaaata aaagagtccg gtgaagttca
    17101 tcggattttt gtattatact caatgaaaat caaagagtaa actaggaagc tagccgcagg
    17161 ttgctcaaaa cactgttttg aggttgtaga tgaaactgac gaagtcagct caaaacatgg
    17221 ttttgaggtt gtagatgaaa ctgacgaagt cagtaaccat acctacggta aggcgacgct
    17281 gacgtggttt gaagagattt tcgaagagta ttagcgctaa tttttagggc tttcacttga
    17341 cttttaaaga ctggcaaact tttctgatga ttttcatgaa gagcctgcgc ttttatggta
    17401 aaatagtaac agaataaaag aggagagaaa taatgaaacg tagtatgtat gctggtcgtg
    17461 ttcgtgagga acacatcgga caagaaataa ccttgaaagg atgggttggc cgtcgtcgtg
    17521 accttggtgg tttgatcttt atcgatcttc gtgaccgtga aggaatcatg cagttggtta
    17581 tcaaccctga aaaagtatct gcagaggtta tggcaacagc tgaaagcctt cgtagcgaat
    17641 ttgttattga ggtgactggt caggtcgctg cgcgtgagca agccaatgat aagttgccaa
    17701 ctggtgcggt tgagttaaac gtgacagctc ttattgtgct taacacagct aagacaacac
    17761 catttgagat taaggatggc attgaggcaa atgacgatac acgtttgcgt taccgttacc
    17821 ttgaccttcg tcgtccagaa atgttggaaa atcttaaact tcgtgccaag gtgacccact
    17881 ctatccgcaa ctatttggat gagttggagt ttatcgacgt ggagacacca ttcctttcta
    17941 agtcaacgcc tgaaggggcg cgtgattatt tagtgccgtc tcgtgttaat aaggggcatt
    18001 tttacgctct tcctcaaagt ccacaaatca cgaaacagct cttgatgaat gctggttttg
    18061 accgttacta ccaaatcgtt aaatgtttcc gtgacgagga cttgcgtgga gaccgccagc
    18121 ctgaatttac tcaggtcgac ttggaaacgt ccttccttac tgagaaagaa atccaagata
    18181 ttacagaagg cttgatcgcg cgcgtgatga aggaaacaaa aggcatcgaa gtaacgctac
    18241 cgttccctcg tatgaaatac gatgatgcta tggctcttta cggttctgac aagccagata
    18301 ctcgttttga catgttgctt caggacttga cagaagtggt caaaggtgta gactttaaag
    18361 tcttttcaga agcacctgct gtaaaagcga ttgtggtcaa aggagctgcg gacaactatt
    18421 cacgtaaaga catcgacaag atgacggaag tagccaaaca gtatggtgcc aaaggtcttg
    18481 cttgggtcaa ggtggttgat ggagaattaa acggaccagt tgccaagttc ttgactggta
    18541 tccaagaaga attgacaaca gcgcttgctc ttgaagataa ggacttggtt ctctttgtgg
    18601 cggatacgct tgaagtggct aatgcaacac tgggtgccct tcgtggacgt attgctaaag
    18661 agcttggctt gattgataat gataagttca acttcctttg ggtggttgac tggccgatgt
    18721 ttgaatggtc tgaagaagaa ggccgctaca tgagcgccca ccatccattc acacttccac
    18781 aggaagagac tgctcacgaa ttagaaggtg atttggctaa ggttcgtgcc attgcttacg
    18841 atatcgtctt gaacggttat gagcttggtg gtggtagcct tcgtatcaac caaaaagacc
    18901 ttcaagaacg catgttcaag gctcttggtt tctcaactga agaagccaat gaccagtttg
    18961 gtttccttct tgaagccatg gactatggtt tcccaccaca cggtggtttg gctatcgggc
    19021 ttgaccgttt tgtcatgttg cttgctggag aagaaaatat ccgtgaagtc attgcctttc
    19081 ctaagaacaa caaggcaact gacccaatga cacaagctcc atcaacagtc gctctcaaac
    19141 aactagagga actcagctta caagtagaag aagatgaaac aaacaaaacg aattaagcgg
    19201 tggcgctatt atctgcgccg ctttgcttat cagataaaaa ttttacgtgt cttacaaagt
    19261 atctctcgag aaaagtatga tgagaagatt tcggcctctc tggtctatgg ttttttatca
    19321 gcagtagcag ttaatttctt tttccaacca gggcatgtgt attcgagtgg tgcaacaggt
    19381 ctggcacaga ttatctctgc cttgagtaat cactggtttg gttttcatat tccgatttcg
    19441 ctaagcttct acgccattaa cttccctttg atggtcttag cttggtatca gattggccat
    19501 aagttcaccg tctttacctt tatcacggta tctatgagtt ccttctttat ccagtttgtc
    19561 cctgtggcaa ccttgacgga ggatcccatt atcaattccc tttttggggg tgttgttatg
    19621 ggtttgggga ttggttttgc tcttcgaaac aatatctcca gtggtgggac ggatatcgtc
    19681 agcctgacta ttcgcaagaa aacgggtaag aatgtcggta gtatttcttt cttggtaaat
    19741 ggaactatca tgctgatagc aggtttgacc tttggttgga aatacgctct ttattctatg
    19801 attaccatct ttgtctctag ccgtgtgaca gacgcagtct ttactaagca aaagcgtatg
    19861 caggccatga ttgtgacaaa tcatccagag aaggtaattg aaaaaatcca taaaaaattg
    19921 caccgcggag caaccatgat ccacgatgca gaaggaacct ataatcacga gagaaaggca
    19981 gttttaatca ctgtcattac acgtgcagag tttaatgaat ttaaacagat tatgacacaa
    20041 gtggatccaa gctcctttgt ctctgtctcg gaaaacgttc atattctagg aagatttgtt
    20101 gagatagata attagtgatt aatactcttc gaaaatctct tcaaaccacg tcagcttcac
    20161 cttgccgtat atatgttact gacttcgtca gttctatctg caacctcaaa accatgtttt
    20221 gagctgactt cgtcagttct atctgcaacc tcaaaaccat gttttgagct gacttcgtca
    20281 gtttcatcta caacctcaaa accatgtttt gagctgactt cgtcagttct atccacaacc
    20341 tcaaaacagt gttttgagca acctgcggct agcttcctag tttgcacttt gattttcatt
    20401 gagtataaaa agatcttggg aaaagactca atttcagtag aaaaggaagt aaatcttccc
    20461 acaataaaac gcatagtatc aagttttttc aatacctgat agtatgcgtt tttgctttta
    20521 acgactcttt gcccagtctc ttatttttcg ataattttgt gggcgatcaa ttgacggtaa
    20581 caaatttgtt tattgttttt attatcatta ataatctgga gaatagtttc aagggaaaca
    20641 cgaccaagct ctaaactatt gatatcgaca taggctgcca agttgagctt gggattaacc
    20701 gagtcaaagc tgagaacagg gacatccagc tggtgtttgg caatatagtt acaaacacct
    20761 tcagctagga ggctatcggt tgtgatgata gcatcaattt gtggatcgtg cttgaataat
    20821 cgcttgctaa atttatagcc cttttcttcc agaaactcgt cggcaaagta gatgcgattg
    20881 ttgtcagtgg taagtttgta atgtttaagc gcctgttcat agcccgttaa acggtctttg
    20941 gtcacgaaga gctttttact tcctccgata aaggcaatgc gtttgcagcc ttttttgatg
    21001 aaatattcag tcgcatcaaa accagcttga acattgtcgt tgtcgacaag tgggatgaaa
    21061 ggagatagag atttacctaa gataaggaag gggaactgtt cttctgcgac gagttttacg
    21121 agagggtctt cttcttgggc atagagaaag attagcccat ctacacgctt gccgtagacc
    21181 atttgtgaaa tagcgttgag acgctccttc tcatctttcc ctgttgctat ttgaatggca
    21241 tagtggtttt cagatgcgac ttgagagatg ccacgtagaa ccgatggaaa gaaaggattc
    21301 tggtagaagg cgtctgagtc atcaggaaga actaatccga taacctgagt atagctgctt
    21361 accaagctac gagcgttaag gtttgggtgg tagttgagtt ccttcatagc tttgcgaacg
    21421 cgtttttttg tttcgtcgct aatggttgat ttattttgaa taacacgggt tacggttgaa
    21481 ggcgaaacac cagcagcctt ggccacgtct ttaatcgtaa cgggcataaa aatctcctat
    21541 ttatggtaat ttggatcacg gaaatgtgtt ttataaaaga tagtgaccag atacagaaca
    21601 aaaagaatat tttgtacagt aatcagggtt gtcatatttt ggccaaataa tcccaaaata
    21661 agtgtaatca gagtcggcaa tcctaaacag ttcaagataa agtgatagca ctctttaaag
    21721 gttctaaatg aaaagaggcg tgatttctta gtgatataaa ggagaagact agctcctaga
    21781 gagacgataa agaaattcaa accaaagagg aagctcgcac cgagaactag gaagaggctg
    21841 atatagacac gattttgttg gtaccagtct ttagaaattg cttgggtcaa gctgtctttg
    21901 cttttgaaac tctcagtctg aatggctcgg taagagatgc gggtcagttc cttgctttcc
    21961 ttgctgatga ctagttcatt tgtatcgaaa tgcagttgca agtccttagg taattccttg
    22021 atttgacctg gaccaatcac aacagaaggg gcttgactag ctgttccagt ataagttaat
    22081 gtgccatcga caattgtagc atgttcagag agatcctgga caacctcatt tgtcagaggt
    22141 tcatagacat tatcgataaa agtttctagc ggataggtct cctgggagct gttttggata
    22201 gcaatgggta ccatagacaa gctgataagg aagatactgg taaagagaag ttgaaaccag
    22261 ttgagctcga aacgtttgga caggggctta cgaaaccccc aaatacttga aaaataggaa
    22321 aatggatatg gaagcatagg tatctttcta aaaggtgttt tctatatgag tattattata
    22381 tagagaaatg cgctttattt caagtctagg gggtaaattg cttgctacaa ggatattatt
    22441 tacagtgaca atcgctaata gtcaatgaaa atcaaagtgc aaaccaggaa gctagctgca
    22501 ggttgctcaa agcacaactt tgaggttgga gataaagctg acgtggcttg aagagatttt
    22561 cgaagagtat aaaatgaaaa agggtggcgg ggataaatta tcccttgtcg ccaccacttg
    22621 taagtcctga aacaaagttc ttttgtagga agaagaagag aatacagatt ggaagggcga
    22681 tgaggatagc acctgctgag aagtaggcaa tcttcaagtt tttcgcattg ttaacgaagg
    22741 tttggagacc tacggcaaca gtaaagtatt ctttctcacg aagcaagaaa ctagagagga
    22801 tgtagtcccc gaaaggtccc atgaaggccc agagagcttg tacggcaacc attgggcgaa
    22861 caagtggtag aacaatttgc cagaagcggc ggaagtgtcc tgcaccgtct agttttgcag
    22921 attcgtctaa agacattggc actgtatcga agtagccttt catgagccaa gcattcatcg
    22981 ggataccacc accaacgtag aggaagatga ggaaccagtt gtggttaagg gcgttcaaca
    23041 taagcgccat aacgaagaag gctgtcaaag cggccatagt tggcaccatt tggatgatca
    23101 agaagaagac caaactttgt ttacgagcca agaagttgta acggctgtaa gcataaccag
    23161 caagtacgat gatacttgtt tgaacagcca tggtaattaa ggcgataatc aaagtgttga
    23221 ggtaccaagt accgtacaag gtttcagtga agaggccttt aaagttatca aaattgaggt
    23281 cgatattagt atctagttta aaggctgaga cgttacctgc tttaaaggct gacatgatgg
    23341 taatcaacag tggatagata attacaattg atagaccaat caggtaaagg taagtaaggc
    23401 tttgagtcag tctacgtttg agtttaattg agttattcat cttagacgtc ctccatatca
    23461 aatgcgtgta gtttcttgaa tgcgatcata gagattgaga tgacaatgat agagataatc
    23521 aaggtaacag ctgccgccat tgagtattga ggagatgtac ctgttgtcaa acggtagatc
    23581 catgagatca agatatcggt tgaaccagct ccacctccga cactaccagg tcctccacca
    23641 ttgaagaggt acatgataga gaagttgtta aagttgaagg tgtattggct aatcaaagta
    23701 ggtgccgcaa cagccaaaat cattgggaaa gtgatgttgc ggaatttttg ccaagcgttg
    23761 gcaccgtcaa tataagctgc ttcgtaaagg tcgttaggaa tagattgcaa gatacccaaa
    23821 gtcaaaacgt agatgtatgg gaatccgagc caaccttgca tcataatcaa ggcaatctta
    23881 gtccaagttg ggtctgtttt ccaaggaata agagctccat caaggaaagg aaggaattta
    23941 gccaagattg gcaatacttg agtgttgata gcaccgacac tatcgttaaa catgtttgag
    24001 aatgtcaaga tagtgatgaa ggctgggaca gcccaaggaa gaaggaaaat aacaccaaag
    24061 atacgttttc ctttgataaa tggttggttg gcaatgatag ctgtgaagat accaattacg
    24121 atttgtaaag tagaagctgc caaagcccaa atgatagtcc aagaaagaac agaaccaaag
    24181 gcagaacgga aggtactcaa gctccaaatg tttgtaaagt tggtcaaacc aacccagtcc
    24241 aacaacttgt ttggtggcaa gtgttggaag tcgtagttgg taaaggcgat catcaaggtt
    24301 acgataactg ggaagataat cgcgaaggtc atggcaacat aagatggaat gatcaagagg
    24361 taagggaagc cattttcata aatccctttg atcatgtctt tgagtgtgcg tggaactgga
    24421 attccattgt taatgcgttt tgcaatcgta tgtgcatctt tgatatttga gaaataaaag
    24481 agtacataaa cgattacaaa gattagatgg aaggcaccac gaatcagcat aaagagggaa
    24541 ttatcacgac ctggtttgtc accaagagtg atgaggttgc tcaattcagg ggttgcaagt
    24601 gctaggaagt aaaggacaaa tacgatggtt acaccaagga agataaaacc tttggctttt
    24661 tgtttattgt aaatctgtcc taacccagga atgatagaca gcagggctgc tttactaggt
    24721 tgttgctttt ccatattaac tcctttcata ggatactact tttttataag aaagctaaat
    24781 tatttgtgtc tctattgata aattcaaagg gggatttgat ttccaccccc cttgaacaaa
    24841 ttttttattc accaaatttt tgtttgattg tttctttgat caatgttaca gcatcgttag
    24901 cagctgtttt agcatctttt tgaccactta cagcatcaaa gagcatattt ttcgctggat
    24961 cccaaactgc agacatttga gagatgtttg gcagtggttg agtgttcttg aactgtttga
    25021 taacagctgt tgtcaactca tcgtttttac cttcagcgta tgaacgagcc tcagtattag
    25081 ctgggatttc gttagtctta tcatataata ctttttgttg ttcagttgca acaaggaagt
    25141 ctacaaattt ttgagaagct tcaaggttct taacggcttg aggaatgacc caagctttac
    25201 caccaccgaa tgcagcatat tcttttccat ttggaagagt tgggatagtt gcaactccgt
    25261 agtttacttt agcatcttta aaggcttgag ctttccaagg tccgtcgatg atagcagctg
    25321 ttttaccttc ttggaattga gtttggatta agtttccagc accttctgta tcttgcatac
    25381 ctttaggcca tttttcgtac caagatttag cgtagttgat acctgcgata gaaccgtcgt
    25441 ttgcaagacc gatgtcttta gcgtctttac cgttttggcc aaagacgtaa gcaccgttac
    25501 cggcaagaag tccatatgta tagtagaagt ttgtccagtc agctaggaag gcagtagttt
    25561 taccatcttc accagcgaat gcgtatttgc tatctttagc aaggttttcc aagtcagcaa
    25621 atgtttttgg agcatctttc accaagtctt tgttgtagta cataacaagt gactcgataa
    25681 cggcaggagc accgtaaact ttaccattag cagctgttac aagagattta gttgtgtcgt
    25741 ctgttttagc accatcgctc aatttcactt ctgaaagttg tccgtcagaa ccaaggctac
    25801 ctacacggtc gtatggagcc atcataacat cagggacatt accagattgg ttgtcaagag
    25861 aaagtttatc aagacctcct agagcatcac cagttttaag agtgactttt actccagctt
    25921 ctttttcata agctttagca acctcttcaa tatagctctt atatccctcg tctacatata
    25981 cagtgagttc tttgacttca gatgaaccag aatcagcagg cttatcagca gttttgcttc
    26041 cgcaagctac caaaagcaag ctagcaagtg tagcagttcc aagcaccgca gtgctcttca
    26101 taaatttaga tgacatagtg tattcctccc aaagaatagc aagttttatt gataaggaaa
    26161 cgcaaacgtt ttcctttatg agcttagtat agcacaaata gaaaacggtt gcaagtattt
    26221 tttgtaaaaa ttttaaaaaa tttttaagct tgatttgata gcataatttt gcattttaat
    26281 agaagaaaag tatgaaatag ataagaatac agtattttta ataaaaatat atggaatctg
    26341 tctaaagagg gaaatcagga ggggctacct ccctgtttct aatccctatc tattttctat
    26401 agacgtttgt gctttgttgc atataaattc aagctcccta aggaggtttt gtactttgta
    26461 gactgtctcg tgagagtcaa actttaggat tagatagttt taagttatct ttgctaaaat
    26521 gagttagtct ccttaaaaat aatcaaaaaa gttttttaaa aacgcttgca attatgcttg
    26581 aaaaggagta tacttataag taacgcaaac gtttgcgtct gcaaaatacg caacgttcca
    26641 ttattttaac acacgaggtg ctattatgaa aaaacgtcaa agtggtgtgt tgatgcacat
    26701 ctcttctctt ccaggagctt acggaatcgg atcatttggt caaagtgctt acgacttcgt
    26761 tgatttcttg gtccgtacaa aacaacgtta ctggcaaatc cttccattag gagcaactag
    26821 ttacggggat tctccttacc aatctttctc agccttcgca ggaaacactc attttatcga
    26881 tttagatatc ttggtggagc aaggtttgtt ggaagcaagt gaccttgaag gagttgactt
    26941 tggtagcgat gcgtctgaag ttgactatgc taaaatctac tatgcacgtc gtcctctttt
    27001 agaaaaagcg gtgaaacgtt tctttgaagt cggagatgtt aaagattttg agaaatttgc
    27061 tcaagacaac caatcatggc ttgagctctt tgctgagtat atggctatca aagagtattt
    27121 tgacaatctt gcttggactg aatggccaga tgcagatgct cgtgctcgta aagcttcagc
    27181 acttgaaagc tatcgtgagc aattggcaga caagttggtt taccaccgtg tgactcaata
    27241 cttcttcttc caacaatggt tgaaattgaa agcttacgct aacgacaacc acatcgaaat
    27301 cgttggggac atgccaatct acgtagcgga agattcaagt gatatgtggg caaatccaca
    27361 tctcttcaaa acagatgtca atggtaaggc tacttgtatc gcaggatgcc caccagatga
    27421 gttttctgta actggtcagc tttggggtaa tccaatctat gactgggaag caatggacaa
    27481 agacggctac aaatggtgga ttgaacgctt gcgtgaaagc ttcaaaatct acgatatcgt
    27541 tcgtatcgac cacttccgtg gcttcgaatc ttactgggaa atccctgctg gttccgatac
    27601 agcagcacct ggtgagtggg tgaaaggtcc aggctacaag ctttttgcag ccgttaagga
    27661 agaacttggt gagctaaaca tcatcgcaga agaccttggc ttcatgacag atgaagtgat
    27721 cgaattgcgt gaacgtactg gcttcccagg aatgaagatt cttcaatttg ccttcaaccc
    27781 agaagacgaa agcattgata gcccacactt ggcacctgct aactcagtta tgtacacagg
    27841 aacacacgat aacaatacgg ttcttggttg gtaccgtaat gagattgatg atgcgactcg
    27901 tgagtacatg gctcgttaca cgaaccgtaa agaatacgaa acagtggtac acgctatgct
    27961 tcgtacagta ttttcatcag ttagctttat ggcaattgca actatgcaag atttactaga
    28021 attggatgag gcagctcgta tgaacttccc atctaccctt ggtggaaact ggtcttggcg
    28081 tatgactgaa gatcaattga caccagctgt cgaggaaggt ttgcttgact tgacaacaat
    28141 ttatcgccga attaatgaaa atttggtaga tttaaagaaa taagacaata atcaggagac
    28201 aactaaacat gttatcacta caagaatttg tacaaaatcg ttacaataaa accattgcag
    28261 aatgtagcaa tgaagagctt taccttgctc ttcttaacta cagcaagctt gcaagcagcc
    28321 aaaaaccagt caacactggt aagaaaaaag tttactacat ctcagctgag ttcttgattg
    28381 gtaaactctt gtcaaacaac ttgattaacc ttggtcttta cgacgatgtt aaaaaagaac
    28441 ttgcagctgc aggtaaagac ttgatcgaag ttgaagaagt tgaattggaa ccatctcttg
    28501 gtaatggtgg tttgggacgt ttggctgcct gctttatcga ctcaattgct actcttggtt
    28561 tgaatggtga cggtgttggt cttaactacc actttggtct tttccaacaa gttcttaaaa
    28621 acaaccaaca agaaacaatt ccaaatgcat ggttgacaga gcaaaactgg ttggttcgct
    28681 caagccgtag ctaccaagta ccatttgcag actttacttt gacatcaact ctttacgata
    28741 ttgatgttac tggttatgaa acagcgacta aaaaccgctt gcgtttgttt gacttggatt
    28801 cagttgattc ttctattatt aaagatggta tcaactttga caagacagat atcgctcgca
    28861 acttaactct cttcctttac ccagatgata gtgaccgtca aggtgaattg ctccgtatct
    28921 tccaacaata cttcatggtt tcaaacggtg cgcaattgat catcgacgaa gcaatcgaaa
    28981 aaggaagcaa cttgcatgac cttgctgact acgcagttgt ccaaatcaac gatactcacc
    29041 catcaatggt gattcctgaa ttgattcgtc ttttgactgc acgtggtatc gagcttgacg
    29101 aagcaatctc aattgttcgt agcatgactg cctacactaa ccacacaatc cttgctgagg
    29161 cgcttgaaaa atggcctctt gaattcttgc aagaagtggt tcctcacttg gtaccaatca
    29221 tcgaagaatt ggaccgtcgt gtgaaggcag agtacaaaga tccagctgtt caaatcatcg
    29281 atgagagcgg acgtgttcac atggctcaca tggatatcca ctacggatac agtgttaacg
    29341 gggttgcagc acttcatact gaaatcttga aaaattctga gttgaaagcc ttctacgacc
    29401 tttacccaga aaagttcaac aacaaaacaa acggtatcac tttccgtcgt tggcttatgc
    29461 atgctaaccc aagattgtct cactacttgg atgagattct tggagatggt tggcaccatg
    29521 aagcagatga gcttgaaaaa cttttgtctt atgaagacaa agcagctgtc aaagaaaaat
    29581 tggaaagcat caaggctcac aacaaacgta aattggctcg tcacttgaaa gaacaccaag
    29641 gtgtggaaat caatccaaat tctatctttg atatccaaat caaacgtctt cacgagtaca
    29701 aacgccaaca aatgaacgct ttgtacgtga tccacaaata ccttgacatc aaagctggta
    29761 acatccctgc tcgtccaatc acaatcttct ttggtggtaa agcagctcca gcctacacaa
    29821 tcgctcaaga cattatccat ttaatccttt gcatgtcaga agttattgct aacgatccag
    29881 cagtagctcc acacttgcaa gtagttatgg ttgaaaacta caacgttact gcagcaagtt
    29941 tccttatccc agcatgtgat atctcagaac aaatctcact tgcttctaaa gaagcttcag
    30001 gtactggtaa catgaaattc atgttgaacg gagctttgac acttggtact atggacggtg
    30061 ctaacgtgga aatcgctgag ttggttggag aagaaaacat ctacatcttc ggtgaagatt
    30121 cagaaactgt tatcgacctt tacgcaaaag cagcttacaa atcaagcgaa ttctacgctc
    30181 gtgaagctat caaaccattg gttgacttca tcgttagtga tgcagttctt gcagctggaa
    30241 acaaagagcg cttggaacgt ctttacaatg aattgatcaa caaagactgg ttcatgactc
    30301 ttcttgactt ggaagactac atcaaagtca aagagcaaat gcttgctgac tacgaagacc
    30361 gtgacgcatg gttggataaa gtcatcgtta acatttctaa agcaggattc ttctcatctg
    30421 accgtacaat cgctcagtat aacgaagaca tctggcactt gaactaatac tcttcgaaaa
    30481 tctcttcaaa ccacgtcagc tttatctgca acctcaaagc ggtgctttga gcaacctgcg
    30541 gctagcttcc tagtttactc tttggttttc attgagtata agatacaaat ttatactaat
    30601 acattttgta aaaaagcgag tttcgattga aattcgcttt tttaaatgct gtagatttgg
    30661 gtcaatcttg tctaaaaata gggaaatcct agatacagtg aagactttaa atgctggttt
    30721 ttactgtcct cagccttata ttttttcgta gttgattacc tcacacctct tgtattcgct
    30781 tacataaagt attataatat aattgtagga aagaaggtgt ttttatgata tacacactta
    30841 aattggtgtt gtttattacc tttcttgtaa taagcttgtt acctgataag atttttggaa
    30901 aaaataaaaa aatttggaaa atagtttttg caatattgac ggcagtggca gcattgtcat
    30961 ttatgtacta agttatttta agaatgtagg gaaataaacc ctacattctt tttagttttt
    31021 tctgttttct aaattctatt tatccaagcg cttcaacatt tcttgcttct tcgcttcaag
    31081 ttctgcacgc ttttcttcga tttcggcatg ttttttctcg agttcagaac aacttgcacc
    31141 attgctaaat tcttttcgcc atcaggagat agggtgagtc gacatgtcta ttactcaccc
    31201 aaagcagtcc tacaaagcag gaattttctg ttactttttt ggaaatagta acgtttatac
    31261 agctttgaca cttcgtatca aagcgccaaa cacactccga gggatttaca gaaagcagaa
    31321 aaggaatgat ctggtataag atcattcctt ttctctcttt ttctttaagt aattatatac
    31381 aatgtacgac gaagtcgtca ttgcaatgct gatccaccac ctaaagggaa ctttaaacaa
    31441 cattgataag ataaagaata taaacaacga aaatacgtta tacccaatta attttattgt
    31501 atatctcatg attaaaagtt aatccttccg ttgttaggaa tggcatcatt tttatcccat
    31561 aattgtgcta aataagtccc cggtgataat aaattcatag cggattctaa agcaacatca
    31621 tttacaaacc aactacctag atatctagaa attgctgaac gaatagcact ttttgctgca
    31681 tgttttcctt ttactttaat tagatttgca aggcctgcag tagttcctcc taatgctaaa
    31741 gctattgtag tatctaatag agcacccatt tgattaactg taataccttg ccaagctgct
    31801 ctaaatggag agtatgtagg tgggattgta taatcgcctt gtaattgtcg gttaattact
    31861 tctttgatcc attgttgtga gacgtctgga tgaaaagatt ggatttcgtt tgcaagtgta
    31921 ttgatttgtt cttctgttag agaagtgaca ggttgaagtt ccatatttgt ttcaatttgc
    31981 gatacttgtt cagaagcgta tacagctgaa acacttggaa tcgctgatac aattaacaca
    32041 attgacgtca aaaaaaccga aataaatttc attaatttgt tcatgagctt ttctcctttt
    32101 tatttgtatc tgcttacatt ttattatata ctgttattat agtcaagaaa aatatgctat
    32161 tatgttaaaa aaatattttt caaaatataa atggacggat ttattttgga ttttatttgt
    32221 tattttgacc tgcctctata ttggtaacca tgatttgttt actctcaatc atcaagaatt
    32281 ctcttttcgt ggtagctttt ggggtttggt actggcctta tatcacttat tgttcattga
    32341 taagtttgta atttcgaatc gaaaataaag attagaacta tgattgactg gctactttta
    32401 atattgattt tggaggaaga ttttgtctct attatttatt attttaaatt tatctatttt
    32461 gtacaatatc tattctttga gacgggagaa atcgaaatac ttgatttata cggcctatat
    32521 catatttggg gtaaatgtag tatatggtat tcaatggtta ttaagagaac tgatttcaac
    32581 tatttcccct taatgtgtat ggaaactata acaaaacgca taagattaag gtttgtgtac
    32641 ctgttcttat gcgtttttgt aatattgaag attttcggta agttttatac ctttgggaag
    32701 tctagtttag aatgctttcc caaccagaat ctctgcttcg atggtaatct ctgtcagttg
    32761 gctccagtca atcttgctct ggtcaacatg aaagaggaga ggagtcatac tgagtagggc
    32821 ttggagttgt tctgctgtga tagtcttagt cagagaggca gtttgactag ataggatggt
    32881 aaagtgttcc tggaaatgct ccttgatatc ttggttagaa tactccttgt ttgtcagctg
    32941 gtcctgtact ctttgacgga tttctttgag gtgattttca gttgggataa cctttatcaa
    33001 gataccgtct ttggataaaa cgcgacgaaa ttctccatag ttggcaggtg agaagatatc
    33061 aagcagaata tccatgttag cgtcttttat aggaagtcgt gccaagtcgc caacgaacca
    33121 attgactgcc cagttgggtt cacttttagc agcgatttgg actgaatctt tggagatgtc
    33181 aaaggcatag aaagtttttt cagagtgact ttcttgtagt ttgcgagaat agaatccttc
    33241 accacaaccg atatccaaaa ttgttgtggt agtttttgag cttgcaagca agtcagatac
    33301 agcatctaag atagcttggt aaaagccggc ttctaggatt tgttgacggt tttgaaaatt
    33361 ttccttgtca tagttagcag attgcttgat ttgaggtgct agattgacat agccaaattt
    33421 cgccaagtca aaagaatgac ggttgcagca cttgaaatta gtctctaaca gagtcagatt
    33481 ttcttgacag ataggacagg caaaggcagt cgcagaagca aaacgctgaa gtttgggctt
    33541 gagatttgta ttcatagttt aagtgtatca aaagaggcaa atgaaagcaa ctttaggaat
    33601 aagtagatgg gagattcagt agaaataaaa ctaattctcc aatttataga atgaaattgc
    33661 ttttatatct aaattgctat ataataatga taaggaggaa ataagtatgt taaaagaagt
    33721 attaaccgtt gcaaaagttg cgaaaaaatc ttcattattt ttgggtggtg tcgcatttgg
    33781 tacccttggt ttgaaaatct tagcaagtaa ggaagctaaa aaaggttatt ctaaagcttt
    33841 ggctaaggct tacaagttga aagacgagct agatgcatct gtttctgttg tgaagcaaca
    33901 tggagacgat gtcttgcaag atgccaaata tttgtacgag caagagaaaa aagaagagca
    33961 attagatagc cttataggtg aataatatgt cttttaaagt gctacataga ggataccaac
    34021 atatccgact atcatcttct ttttcactta ccttggatat tcaagactat cttcgttcct
    34081 tggcgagaga tgaaaagggg attgagtcta tccagtttta catggatcaa cagcacttta
    34141 ctctacgcat aaaagaaggc ttttctgtat tagataatgc agaagccttt ttaaaaagaa
    34201 ttgataaagg gaaagtttct gagttgatga ctcttcccat tcgtagagaa gagagtgctt
    34261 attctattgt ttcaggtgca gcggttaagc gtgtactttt tcgtagtttt gtgccgtatc
    34321 ctattcgcta tatatggact tgttatcagg ctttgggtta tattagagaa gcctatcaaa
    34381 cactagcgcg taaggaacta acgatggaag tcttggactg ttcggctatt ttattgtctt
    34441 tgtttatgaa ccaatccaag acagctagca atatcatgtt tatgcttgat ttggggaatc
    34501 atttagatca gtggtccttg aaaaaaactg caacagattt agaacagagt cttcttgcaa
    34561 aagagagcga tgtattccta gtacagggcg atacggttgt tagtatcaag agttccgatg
    34621 ttcaaatagg agatgtcttg atcttatctc aaggaaatga aattctgttt gatggacaag
    34681 tagtttcagg tttaggtatg gtcaacgaaa gttccttgac aggagagagt tttccagttg
    34741 aaaaaagaga gtctgatttg gtttgtgcaa atacagtatt agaaactgga gagttacgca
    34801 ttcgtgtaac agataatcag atgaacagcc gtattttaca gctgattaag ttgatgaaga
    34861 aatctgaaga aaacaagaaa acgaaacaac gctatttcat caagatggcg gataaagtcg
    34921 ttaaatataa tttcttgggg gctgggctga cttacctatt gacaggttct ttttctaagg
    34981 ctatttcttt cctattggtc gatttctcct gcgctttgaa aatctctact cctgtagctt
    35041 atttgacagt tatcaaggaa gggttgaacc gtgaaatggt gattaaggat ggagatgttc
    35101 tggagaaata tctggaagtt gatactttct tgtttgataa gacaggaaca atcacaacta
    35161 gttatcctat agttgaaaag gtgttacctt ttggagacta tagtgaggaa gatattctca
    35221 gaatcagtgc ctgtcttgag gaacacattt atcatcctat tgctaatgcc atcgtcaagc
    35281 aagctgagat agagggaatt gaacatgagg aaatgcatgg gaaactccaa tatatcgcaa
    35341 gcaaggggat caaatctcat atagatggcc aaccagttct tattgggaat tatgtcttga
    35401 tgcaggatga gcagattcat atcagttcag aacaaaatgc tttaattgaa gagtacaaga
    35461 gtcactacaa tctcttattc ttggcttatc aaaatgaatt gattggaatg ttctgcattc
    35521 atactccttt gagaaaagaa gcaaaaacag ccttggataa acttaaggca caggggaaaa
    35581 aattgattct ggcaacagga gacaccttga ttaggacaga ggaattagtc aaagatttgc
    35641 cctttgatca ggtctataca gacttgaaac ctgatgggaa atttgagtta gtagagaaac
    35701 tgcagaaagc aggtcacact attttgatgg ttggagatgg attgaatgac tcagcggctt
    35761 taactctatc agatatcggt gtggtgatga atgagagtgc agatatttct aagcagatga
    35821 gtgatatctt attgttagat aatcgtttgg atttcttcca agagttggat tcgttatcat
    35881 catctttgca aacactcatc aagaaaaata ttcaagatac cgttgtcgta aatagtagtt
    35941 tgattggctt tggcttattt aattggctca gtccttcaaa tctctctatc ttacataatc
    36001 taacaacctt gcgcattgtc ctgcgtagcc tgtctattaa aaatagatag atgagagtta
    36061 ttaacagcaa aaaggagtta cctttctcga ggttaactcc tttgtttata gataaatgtt
    36121 gaatggttta gtaagtcaat acaaagtatt ttttcttccc acgacggata acagtcagtt
    36181 cattttctaa cttatcagcg tcactcaaga catagtcaag gtcttggatg cggtcgccgt
    36241 ttacgtagat ggctccgttt tggacgtctt cacgggcttg gcgttttgag ttaactatac
    36301 cagatgagac gagcagttcc acgatattgt tgttttcgtc tgcctgtact tggtagtttg
    36361 gcacaccacg aagtccttgt ttgagctctt tgacagaaag gtttttgatg tttcctgcaa
    36421 agagttgctc agtgatgtta agtgcttctt tgtaggcttc ttctccgtga acaagtgtaa
    36481 caacttcacg agccaagact ttttgagcca agcgttcgtg tggcgctgct tcaaattgtt
    36541 tacgaatatc ttcaatctca tcaagtgaca agaaagtaaa gattttcaag aagcgaacag
    36601 cgtcagcgtc catcacgttc atccagaatt ggtacatttc gtatggagaa gtcttttcgg
    36661 gattgagcca gacggcattt ccttctgatt taccaaattt tttaccagtt gcatctgtga
    36721 ttagtggaac agtgataacg tgaccagtct tgtccgcctt acgacgaagc aattcggtac
    36781 cagctgtcat atttccccac tggtcagaac caccgatttg aagagtgaca ttatggtctt
    36841 ggttaaggac gaagaagtcg tacccttgca tgatttggta agcgaactca gtgtaagaaa
    36901 ttcctgtttc gatccgtttt ttaacagatt ccttactcat catgtagttg accgtgaagt
    36961 attttccaat gtcacggagg aagtcaatga agctgatgct gccaaaccag tcgtagttgt
    37021 tgaccatgac agccttgttt tcgccatttt caaagtcaag aaaacgagaa agttgtcctt
    37081 ggatagactt gacccagcca tctactgtgt cttttgtttg gagactacgt tcagcatctt
    37141 tgaaggacgg atctccgatg agacctgtag caccgccaac gagcgcataa ggtttgtgac
    37201 ctgctagttg caagcgacga cttgtcaaga ttgcgacaag gtggcctagg tgaaggctgt
    37261 cagcagttgg atcgtagcca gtataataag aaacttgacc ttcttctagg gctttacgca
    37321 aagcttcttc atcagtcgtt tgaaatatca aaccacgctc ttttagctca tcaaaaatgt
    37381 gcatgtgtct tttctccttt ttaaaattat tgtttctacc tattgtatca caaacttggg
    37441 caatagccta gcagaatagg gaagaaagtg gctattttat tgaaaatttc ccttttgtgg
    37501 tataatagat agagtgagga aatccatgca aaatcaatta aatgaattaa aacgaaaaat
    37561 gctggaattt ttccagcaaa aacaaaaaaa taaaaaatca gctagacctg gcaagaaagg
    37621 ttcaagtacc aaaaaatcta aaaccttaga taagtcagcc attttcccag ctattttact
    37681 gagtataaaa gccttattta acttactctt tgtactcggt tttctaggag gaatgttggg
    37741 agttgggatt gctttggggt acggagtggc cttatttgac aaggttcggg tgcctcagac
    37801 agaagaattg gtgaatcagg tcaaggacat ctcttctatt tcagagatta cctattcgga
    37861 cgggacggtg attgcttcca tagagagtga tttgttgcgc acttctatct catctgagca
    37921 aatttcggaa aatctgaaga aggctatcat tgcgacagaa gatgaacact ttaaagaaca
    37981 taagggtgta gtacccaagg cggtgattcg tgcgaccttg gggaaatttg taggtttggg
    38041 ttcctctagt gggggttcaa ccttgaccca gcaactaatt aaacagcagg ttgttgggga
    38101 tgcgccgacc ttggctcgta aggcggcaga gattgtggat gctcttgcct tggaacgcgc
    38161 catgaataaa gatgagattt taacgaccta tctcaatgtg gctccctttg gccgaaataa
    38221 taagggacag aatattgcag gggctcggca agcagctgag ggaattttcg gtgtagatgc
    38281 cagtcagttg actgttcctc aagcagcatt tttagcagga ctcccacaga gtcccattac
    38341 ttactctcct tatgaaaata ctggggagtt gaagagtgat gaagacctag aaattggctt
    38401 aagacgggct aaggcagttc tttacagtat gtatcgtaca ggtgcattaa gcaaagacga
    38461 gtattctcag tacaaggatt atgaccttaa acaggacttt ttaccatcgg gcacggttac
    38521 aggaatttca cgagactatt tatactttac aactttggca gaagctcaag aacgtatgta
    38581 tgactatcta gctcagagag acaatgtctc cgctaaggag ttgaaaaatg aggcaactca
    38641 gaagttttat cgagatttgg cagccaagga aattgaaaat ggtggttata agattactac
    38701 taccatagat cagaaaattc attctgccat gcaaagtgcg gttgctgatt atggctatct
    38761 tttagacgat ggaacaggtc gtgtagaagt agggaatgtc ttgatggaca accaaacagg
    38821 tgctattcta ggctttgtag gtggtcgtaa ttatcaagaa aatcaaaata atcatgcctt
    38881 tgataccaaa cgttcgccag cttctactac caagcccttg ctggcctacg gtattgctat
    38941 tgaccagggc ttgatgggaa gtgaaacgat tctatctaac tatccaacaa actttgctaa
    39001 tggcaatccg attatgtatg ctaatagcaa gggaacagga atgatgacct tgggagaagc
    39061 tctgaactat tcatggaata tccctgctta ctggacctat cgtatgctcc gtgaaaaggg
    39121 tgttgatgtc aagggttata tggaaaagat gggttacgag attcctgagt acggtattga
    39181 gagcttgcca atgggtggtg gtattgaagt cacagttgcc cagcatacca atggctatca
    39241 gaccttagct aataatggag tttatcatca gaagcatgtg atttcaaaga ttgaagcagc
    39301 agatggtaga gtggtgtatg agtatcagga taaaccggtt caagtctatt caaaagctac
    39361 tgcgacgatt atgcagggat tgctacgaga agttctatcc tctcgtgtga caacaacctt
    39421 caagtctaac ctgacttctt taaatcctac tctggctaat gcagattgga ttgggaagac
    39481 tggtacaacc aaccaagacg aaaatatgtg gctcatgctt tcgacaccta gattaaccct
    39541 aggtggctgg attgggcatg atgataatca ttcattgtca cgtagagcag gttattctaa
    39601 taactctaat tacatggctc atctggtaaa tgcgattcag caagcttccc caagcatttg
    39661 ggggaacgag cgctttgctt tagatcctag tgtagtgaaa tcggaagtct tgaaatcaac
    39721 aggtcaaaaa ccagggaagg tttctgttga aggaaaagaa gtagaggtca caggttcgac
    39781 tgttaccagc tattgggcta ataagtcagg agcgccagcg acaagttatc gctttgctat
    39841 tggcggaagt gatgcggatt atcagaatgc ttggtctagt attgtgggga gtctaccaac
    39901 tccatccagc tccagcagtt caagtagtag ttctagcgat agcagtaact caagtactac
    39961 acgaccttct tcttcaaggg cgagacgata attctctaaa tgaagtggcc aatcaatgga
    40021 ttgccacttt tttcctttcg tgttacaata agggtatgaa tcagtatcag aaaaagattg
    40081 ttaacggaaa aatttattcg ctcctatccg gcttaatatg gggaatctgt ggaattttag
    40141 gagagtactt ctttactcat tatcaggtgt cttcgggctg gattacctct atgcgtttga
    40201 cactggcagg gagtcttgta ctcatttggt ctgcaataca attaaaatcg caagtgctag
    40261 atatttggcg agacaagaaa aattacctgc cctttttagc ctatgctatt ttggggattt
    40321 tttcagttca gtattttttc tatctctgtg tagaatactc aaatgctacg acagcaacta
    40381 ttttacagtt tattagccct gtttttatcc tcttttacaa tcgcttggtt tatcaaaaac
    40441 gagcgtcaaa aagcgctgtt ttctatgttt tggttgccat gctgggtgtt tgcttgatgg
    40501 cgacaaaggg agatctctct cagttatcca tgacgccgct agctcttata acaggtttgc
    40561 tgagtgccat gggtgttatg tttaatgtta tcttgcccca accttttgct aagcgttatg
    40621 gttttgttcc tacggttggg tgggggatga ttttggcagg tttgtttagc aatgtcctct
    40681 cgccggttta tcagctttcc tttactcttg atatttggag tatcttgatt tgcctcatta
    40741 tcgctttctt tggaacggct tttgcttttt tcatttccat gaaggctgtg tccttggttt
    40801 ctcctttggt ggttgccgtt atcagtgcca gtgaacctct ctcttctgct ctcttgagtg
    40861 ttttgttctt aggattagta gtggattggt ccctccttct agctatagcc ttgattattt
    40921 tacccatgat atttttgtct atagaagaag cgaaagaaag tagataaaaa gtcttttctt
    40981 aattctaaaa gtgtgctata ctagaatagt caataaaaca cggggagata gttgtgaaga
    41041 gtgtttttag gttgtatcgg taggggcttg cttttaccga cagcacgttt tatctcacat
    41101 ccctaaaaaa tcatacctaa aaacagacaa aaaggtttca aatttgaggc cttttttggt
    41161 agaataggta tcattataac gaaccaggag gcacctatga ctgctacaaa aatgaacgct
    41221 caagaaatta tccaatttat cgccaatgct gaaaagaaaa ccagtgtcaa agtaaccttt
    41281 gaggggcaac tcgcaactgc tgtgcctagc tctgttgtca aactaggaaa tgtcctattc
    41341 ggagactgga aggacgtggc tccgcttctt gaaggtttgg tagaaaatca agattatgtt
    41401 gtcgagcaag atgctcgtaa ttctgcagtt cctttgctag ataagcgtgc tatcaacgct
    41461 cgtatcgagc caggtgctat tatccgtgac caggtggaaa ttggtgacaa tgctgttatc
    41521 atgatgggag ctgttatcaa tatcggtgct gaaatcggtg ctggaaccat gattgacatg
    41581 ggtgccatcc ttggtggccg tgctatcgtt ggaaaaaata gccacgttgg tgcaggtgca
    41641 gttttggcag gtgtgattga gccagctagt gctgaaccag tccgtgtcgg agacaatgtt
    41701 cttatcggtg ctaatgcagt ggttatcgaa ggagtccaaa tcggtagtgg ttcagttgtc
    41761 gctgcaggag ctatcgttac ccaagatgtc ccagaaaacg tggtagtagc aggtgttcca
    41821 gctcgtatca tcaaagaaat tgatgcccaa acccaacaaa aaacagcgct agaggatgcg
    41881 cttcgtacct tgtaattgta aaagtaaaaa agaggcggaa ccctttttcc agcctctttc
    41941 tgctataacg gaggacagat agatgttaga tttgattcag actagacgag ctttacacca
    42001 gattccagag attggcttgg aggagttcaa gactcaggct tatttgctgg atgtgattga
    42061 gaaattgact acgggcaagg attttgttca aattcgtact tggcggacag ggattttggt
    42121 ctacctgcag ggaagtcagc cggagcgaac cattggttgg cggacagata ttgatggtct
    42181 gcctatcgtc gaacaaacag gtctaccttt tgcttctcaa caccaaggtc gcatgcatgc
    42241 ctgtggccat gattttcata tgaccattgc cttaggctgt cttgagcgcg cccttgagga
    42301 gcaacccaag aataatctgc tctttctatt tcagcctgct gaagaaaatg aagctggtgg
    42361 catgctcatg tatgaggacg gtgcttttgg agattggttg ccaaaccaat tctatggtct
    42421 ccatgttcgt ccagatttga aagtcggcca gattgcgact aatactcata cactctttgc
    42481 aggaacttgc gaggtgaaga tccgtttcaa aggcaaaggt ggccacgcag cttttcctca
    42541 tgaagccaat gacgccttgg tggcggctag ttactttgtg acccaagtgc agtcagttgt
    42601 tagccgcaat gtcaacccaa tcgagggagc agtggtgacc ttcggcgttt ttcaagctgg
    42661 aaccaccaac aatgtcatca cagacacagc ttttttacat ggaactattc gtgccttgac
    42721 acaggacatg agtctcttgg tgcaaaagag ggtaaaaaca gttgcagaag gggttgcagc
    42781 tgcctttgat atggaagtcg aagtggaact caaacaaggt ggatacctac ctgttgagaa
    42841 caatccagcc ttggcgcgtg aactgatgga cttctttgac gaaaaagacg gaatcgagtt
    42901 gattgatatc gaacctgcta tgacaggcga ggactttggt tatctccttt caaaggttga
    42961 tggcgtgatg ttttggctag gtatcgatag tccctacgct cttcatcacc ctcaaatgag
    43021 tcctaaggaa gaagtcttag ctattggggt ggctgcggtg tctagtttcc tgaaaaagaa
    43081 ggcagcagaa tagagggctt gtctatgaaa tcggaattac gcaagcaagt cttgcatgaa
    43141 atgaaggcta tatctcaaga gcaaaaacag gctatagacc aagctttaac cgagcggctt
    43201 ttacaccatc ccttctacca agaagccaag gtcatcgcaa cctacctctc tttttctcat
    43261 gagtttcaaa cgcgggaact gattgagcag gcgctgaagg acggcaagaa ggttttaata
    43321 cccaaaactt atcccaaggg gcgcatggag tttgtggtct atgatccgca acagttggta
    43381 aaaacttcct ttgccttact ggagccacag ggagatgtgg aagtggtgga tgcatctcag
    43441 attgatttga ttcatgttcc tggtctggct tttacgacga aaggatatcg gataggatac
    43501 ggtggaggct attatgaccg ctatctggaa catttttctg gtcgtacttt gagtacgata
    43561 tatccttgtc aaattcagga ctttatacct gaaaaccatg atattcctgt tcaggaggta
    43621 ttaattgatg aaggaaatct ttgatagacg ttaccctgtg acgagtttct tcctcttagt
    43681 gacggccttg gtatttttac taatgttggt cactgcaggc ggaaactttg acagggcaga
    43741 tacattattt cgatttggag ccatgtatgg gccagctatt cgcctctttc ccgagcaggt
    43801 ttggcgtctc ttgtctgcca tttttgttca tattgggtgg gaacatttca ttgttaatat
    43861 gctttcactt tattatcttg gaaggcaggt agaggagatt ttcggttcta agcagttttt
    43921 ctttctctat cttttatcag gaatgatggg caatctcttt gtttttgtat ttagtcctaa
    43981 atccttagca gcaggcgctt ctacctctct ttatgggcta tttgccgcga ttattgttct
    44041 tcgctatgca actcgcaatc cttatattca acagctaggg caatcctatc tgacactttt
    44101 tgtggttaac attattggaa gtgttctgat tccaggaatc agcctagcag gccatatcgg
    44161 tggtgcagtt ggtggcgcat ttctagcagt tatctttcca gttagaggag aaaaacggat
    44221 gtataatacc agccagagat taggagcggt agtcttgttc gtaggactcg ccattttgct
    44281 tttctacaag ggaatgggaa tgtgacgaag ttgaaagaaa atttgtgtaa tgaaaaaaga
    44341 taaggcattt tttgaacctt atctttttat tttattttat tccttctcag tcaattcttt
    44401 tccaagttgg atgaggtaat tcttcaaatc atctttgact tgtgggtgtt tgagggcgta
    44461 gtcgatggat gttttcatga agccaaactt gtctccgaca tcgtaacgag ccccattgaa
    44521 ctcacgagca aatacacgtt gtgttttatt gagggtgtcg attgcatctg tcagctgaat
    44581 ttcatttcct gcacctggag cttggttttc gaggatttgg aaaatttcag gcgtgaggag
    44641 gtagcgtccg ataatagcaa ggtcgctagg agcgtcctct ggagctggtt tttcaacaaa
    44701 ggtttcaaca ctgtaaagac catcttttcc ttcgccttgc ggagcaataa ccccgtaagc
    44761 agatacttcg tcatgaggga ctggcatgac agcgatagta gacgcgtggg tacgctcgta
    44821 gtcatccatg agttgtttgg taagtggaac agccttttcg tctgtgatat ccatcaagtc
    44881 atcaccaagc ataacgacaa aaggttcatt tccgacgaaa gccttagctt gcaaaacagc
    44941 atctccgaga ccgcgtggat gagtttggcg gataaaatgc agacgcatgt cagttgtttt
    45001 atcaactagc ttcaaaagat ctgttttccc tttttctttg aggttatatt ccaattcgaa
    45061 gtttgaatca aagtggtcct caatagaacg ttttgattta ccagtgacaa ctagaatatc
    45121 ttcaatacct gatttgagag cttcttccac gataaactgg atagttggtt tgtctacgat
    45181 tggcaacatt tctttggcaa gggccttggt tgctggtaaa aatcgagttc ctagtccagc
    45241 agcagggatg actgcctttc taacttttga tgtcataaaa atcctttcta tagagggtta
    45301 agaccactca ttttctgctt taaattcatt gttcatgatg tcataaatgg catctttgat
    45361 attggttccg tgataaataa cttggtaaat agcctgtgta atgggcatat agactccaag
    45421 ttcttgggct agttcatagg ctgctcgagt cgttgaaatt ccttcgatta ccatgcccat
    45481 attagcttct atatcagcta gggattctcc tcgtccgaga gcatctccag ctctccagtt
    45541 acgagagtgg atggaagttc ccgttacgat caaatctccc acaccagata agccgctata
    45601 ggtcaatgga ctggccccga gtgctacccc taggcgggtg atttctgcta aacctcgagc
    45661 gatgatggct gccttagcat tatcaccaaa tccaagacca tgtaaagctc cagcaccgac
    45721 agcaataata tttttaagag caccagcagt ttcaaccccg ataacatccg tattggtata
    45781 aagtcggaag tagtgattac taaatagctt ctgaacgtat tgagctgttt gtaaatcttt
    45841 agaagcagca gttattaaag ttaggtcacg cacaatggtc tcttctgcat gactaggccc
    45901 tgaaacaacg acgatatcac tacggagatg ttcaggaatt tcttcttcaa gaatggttga
    45961 taatcgttta tggctatcag gttctaatcc ctttgatgcg tgcatgatga taaccttatg
    46021 gtccaaggtt tgtgcaactt gctgggcaac aagtcgtgtc acttttgttg ggacaacaaa
    46081 caaaatcgca tccacatctt tcaatgtttc tgctaagtcg gtgtaggcaa tgatattttc
    46141 gtctagaacg acatctttaa agtagtgctt attagtatgg tgtgtattaa tttcattgat
    46201 ttgctcggga agatttcccc aaatacgtac ctcgtgtcca ttgtcattta agacttgtga
    46261 aagggcggtt ccccaagaac caggccccaa gacggcgacg gtttgttttt ccatgatttc
    46321 ctccttgata gagttctttc tcttattcta tcatattcta ggggattttg cacttgcatt
    46381 actggggtat ggtggaaaaa tacacaaaaa ccgagacgaa gggataaact cttagtcttt
    46441 agttaaagtg agaagaaatc tgatagatag tttgattcag tatatgctta taatagaggt
    46501 aaacaactca ggaagttctt gtaggttgcg agagtttggc taaaaaaatc agtcgttcag
    46561 aattttgtgg tgatgagtct gaattaagta tccgtcaatt aattagaatt gaaaatggag
    46621 aatccagacc aacactaaca aagttaaaat atatcgctga acgtttgggg gttgaagatt
    46681 acaagttgat gccaagttat atagagttgg ataaggaata cctagaattg aagtatttct
    46741 tgatgaggac tcctacatac gaagatgaaa ctatcgccca aaagaaagag agtgttttga
    46801 taagattttt gaagagtatt atgataggct acctgaggaa gaaagattta tcatcccaaa
    46861 ttattcatat ctggcactaa cgaactacac agttcaaaaa ttaccagaaa agctagttga
    46921 aatactgtcc ttctggtgat tttgcttaat ttctgatgtt cgagacataa ttgaggagat
    46981 ttgtcttgat tttttcctaa aaaagggcag actcctccct tggtttgatg ctcgattttt
    47041 aaaataaggt gacttattct gttaggcatg ttttcccctg ccctctagct ctatatggaa
    47101 cagggagtat cagatttatc tggcagattg tatcaaataa ggcttgaaac tgttttgaat
    47161 aggcatatag acttgagaat ttgagaatat gtcgtctagc agtagagaca tattttccgg
    47221 caatatgaag gaagagacgt cgaaaacgct tgatagtcaa ggcctttact tcatcaccag
    47281 ctagctgctt taaaaagagg tagagattat aagccagaca gcccatcatc atacgtactt
    47341 cattcttaat catggtcgaa ctatctgtct tatccccaaa gaatcctgct ttcctttctt
    47401 tgataaagtt ttcagcattc cctcgttcac gatagaattg aaacagctcc tcactatcta
    47461 cactgcttaa attgttcaaa tgaacttttt tacaaccata aaaaagagca cttaatcgta
    47521 ctcttctctt ttaaggatta gttgaattaa ttcaaatcca ccagttctcc tgtttctagg
    47581 tagactaggc gttcacaaat gttagcaatg taatcagcga agcgttccag atgccctatg
    47641 atataaaggt attgagttcc attgggaatt gaggtttctt ggtctttcat aagtccaatg
    47701 atttcctttg ataaggcata ataatattgg tcaatctgtt catctttttg agcaatacta
    47761 atagcttttg aggcttggtg caaaggaaag gcaaccaata aatcggctag catgctgagg
    47821 gataatttac ccatttggtg taactgttct tcgtcagggg ctagttgatt ttcttttagt
    47881 tgcaaaacag ctttagcaat gcctgccata tggtctccca tacgttcaag gtctgaacaa
    47941 gaagacatga tgctaatcac aaatcgaagg tcagacactt gtggctgctg caaggccaac
    48001 aaacgggcac aggtcaattc gatagcgctt tgaccttggt tgatagcatg atccttattg
    48061 ataattagct ctgccatctc cttgtctttg gaggctaagg ccagtaaggc ttttgaagct
    48121 gtttcaagga caagttgccc tagtcctaaa aaggattgtt ctaattcatg caattctaag
    48181 tcaaattgat ttctcatagg tttttccttc cttatccgaa ccgtcctgaa atatagtctt
    48241 ctgtgcgctg atcttttgga ttggtaaaca cgtcaacggt atctccaaat tcgcaaattt
    48301 ctcctgttaa gaaaaaagca gttttatctg aaatacgtga agcttgttgc atgttatggg
    48361 taacaatgat aatcgtataa tcctttttta gttgctgaat gaggtcttca atttttaaag
    48421 tggagatagg gtctaaggct gaagtcggct catccattaa cagaatatca ggttctactg
    48481 ctaaagctcg cgcaatgcaa aggcgttgct gctgaccgcc agataaggac atggcactct
    48541 ttttaagatc atctttgact tcttcccaaa tggctgcccc ttttaaagat ttctccacta
    48601 aggcatctaa ttgttttttg tctcgaatac catgtgtcct tgggccataa gccacgttat
    48661 catagataga catggcaaag ggattaggct gttgaaaaac catccctaca cgcttacgta
    48721 gctgattaag gttgaatttg ctactataaa tatcttgctc atctaagagg acttggcctt
    48781 caatatggca agaaggaacc aaatcgttca tccggttaag ggtttttaga aaagttgatt
    48841 tgccacaacc agatgggcct atcaaggcag taatctgtct ttctggtaat tgaatcgaaa
    48901 tattttttaa ggcttgaaaa tccccgtaaa ataagtctag gtgtctgact gaaaatgttc
    48961 ccatactagg aagctccttt cacaagtttt cgagataata agcttgatag agtatttatc
    49021 attaaaacag taataatcaa aatcacaccg gtagcatagg cttcattgac atgtagcccc
    49081 tcacttgaca gcatatacat atgcagggct agagaacgac ctgaagacat gagactactt
    49141 ggcgtattgg tagaggtacc taatgtatac atgagggcag ctgtttcacc aacgatacgg
    49201 ccaatagcta gtatcactcc agctaaaata cctggcatgg caactggtag aacaattcta
    49261 aaaacagtcc gtaacttacc tgccccaagt ccataacttg cttgacgcat gctatcacta
    49321 acagataaaa gggcttcttc tgttgagcga ataatgactg gcaacaccat gataactgag
    49381 gttaagattc ctgataacag agagtattga aaacctaaga agactacaaa gaagagcatg
    49441 ccaaacagac caaaaacaat ggaaggaatc ccagacaagg tatctgaggc caatcgcatg
    49501 attttaacac aaagggaatc tttttttgta tattccacaa gataaaaacc agcaaaaatc
    49561 cctatgggca aggctaaaag aagagcacca aagaccagaa taacggtgga aataatcgct
    49621 ggcataaggg aaatgttctc agaagtataa gtccaagaaa agagggatag acttagatga
    49681 ggtaagcctt tgatgaggat aaaaccaatg attaaaaaga gagagccaaa ggttaaagct
    49741 gaaaaacaat aaacgagaag ttttagcagg tatttactca taagatgatt ttcctttcaa
    49801 gtaggcaaag taggcattaa tcaagagaat aaggaaaaag agaactgctg aggttgcaat
    49861 aagggcttcc ctatgctgac ctgatgcgta agccatctcc agaacaatat tggttgttaa
    49921 ggttctggtt cctgaaaaga gtccacttgg aataattggc tggttgcctg ccaccaaaat
    49981 aactgccatg gtttcaccta ctgcgcgacc gattcctaaa ataactgctg ataaaatacc
    50041 agatctcgca gctggcaaga tgacactaaa aatactccgt tcatgactag ctcctagagc
    50101 caagctacca gaataatacg ttttgggaac tgttcggata gcagattctg acaaactgat
    50161 aatggttggc aaaatcatta ttcctaatag taacgaagcg gttaggacac tcatgccatt
    50221 tcctaaaaag cttctaatcc aaggcaccaa taattgtagg ccgaaaaaac cataaacaat
    50281 agatggaatg gctgccatca agttgatagc tgattttaag aagccataga cgggctttgg
    50341 acaataataa accataaaca ccgatgtcaa gatgcctgtt ggcaccccaa tcacaatcgc
    50401 tcctaaggta attaataagg aaccaacgat cattggtaaa ataccatagc ttgccggaat
    50461 gttcgttggc gaccaatcac tgcctaataa aaaacgggca aagccgtagt tagctatgaa
    50521 aggtaagcca ttactaaaaa taaagaaaca gattagcaaa atagctacaa cagctactgt
    50581 tgcactcatg aaaaaaattg ccctaaaaac tgcttctttg aaggcttgtt ttgtcacatc
    50641 ttgtcctttc tagtgaagaa agtaagggag atacgacacc tccctacttg ccttctttat
    50701 cttattgtac gatgaaacgt ctgcatctct ttagagattt atggagcaaa cattttattt
    50761 aatcttgtcc caggtggtta atttgccact aaaaacgtct gcaagttcag ccatactgac
    50821 ttggcttgcc ttattgtcat tattgaccac aacagcaata ccgtctaaag caatagcatc
    50881 atgggtgaga ctcttacctt cttcaggagt taattcccta gaaaccatac caatatcagc
    50941 ggttttctcc ttaacagcgg taatacctgc tgaagaccca ttagaggtaa tatcaatcgt
    51001 aacttctgga ttttcttttt tataagcttc tgctaatttt tccattaaag aagatactga
    51061 agtggaacct acaacagaca acttgcctga taagtgttgg cttgtatatt ctgtggtttc
    51121 ggttttagct tcaataaatt tattatctgt gaccacttgt tgaccttgtt tggagtggat
    51181 aaagctgata aaatcttgac ctagcttgga aagattagaa gaccaaacaa tgttgaaggg
    51241 acgttgaaga gggtatttac catctaaaac tgtgtctcga ctagccttga caccatcaat
    51301 ctctaaagct ttgacagatt tcgttaaaga tcccaaggag atgtagccga tagcattagc
    51361 attcccttga actgctgaga gaacaccttc tgtactattt tgaatcacag ctgttttggc
    51421 agtgttgtca atttttttat caccgtcttt tttgagaatc cctgtgattt ctgtgaaggc
    51481 accccgtgtt ccagagccat tttctcgtga aatcacctca atcgttcctg aagctgactg
    51541 tttggaagca gctgactgat tgccacaggc aacaagccca aatcctgata agccaatggc
    51601 tgcaagagta agcatttttt tgaatttcat aataatcacc tttatctcta tgtatttttc
    51661 ttgtgtaggc ttactacatt tatagtctaa caagtctttg taaaggttta tccctgattc
    51721 atgtaaagat tgtgtaaaga atcaaaaaaa gccacttttg aaaaatggct gctcctaaaa
    51781 atagctttaa aaattattag tcctgtgcga aagattggtt aggaagaaaa atcgtgaagc
    51841 aactgcctct gccaagctga ctcgtcaccg tgacttggcc acctaataat tgactgagtt
    51901 ctttgacaat ggcaaggcca agaccagtgc caccagtttg tctgcttcga cctttattaa
    51961 ctcggtaaaa acgttcaaaa atacgatcct gctctaattg actaatacca atccctgtat
    52021 ctgatacaga aatcttaatg ccttcgttca ccttttgggt cttgacctca atttttcccc
    52081 cttgttcagt gtaacggatg gcattggata aaagattgag taagatttgg gaaagtaatt
    52141 gactatctga tacgagggtg acatcatctg gcacctgcac ctttagctgt aaatccttct
    52201 tcttgagctg aggttgcaag ctttgagtca aatcctgtac aaattctgcc aaagaaaggg
    52261 tcgtccattg tataggcatt tgttgagcct tagataaggt aagaagatgc tcaacaatat
    52321 gctcaagacg caagctttct ttgtaaataa tgtctagaaa gtcatccttg agcgcttctt
    52381 cttcagctga catcccctta atggtttcag caaagccctt aatcgaagta actggtgtcc
    52441 tcaattcatg ggaggcattt gagacaaagg ctaaatttaa cttttcataa gttctaatcg
    52501 ttgttaaatc atatagcaag acgagcacag cttccacaga ttgggtgggg ctaaaaacgg
    52561 gaactgctgt cacttctaaa atcaagtcac cctcatgaaa cccacttact tcttgtttta
    52621 accttgtttt ttgatcaaag gcttggtgaa ctaaatttcg aatatccatc cgtttgaggt
    52681 catcaagtga acttatgtcg ccgtccacat cgggaaaata atgaggcaga gagcgactgg
    52741 ataataacat ctgaccttga gcggaaacta aaaacgtccc catggttagg tgcgacagaa
    52801 gaacctccat tgtttcggct aggtccttgt attgctgatc ctgttgggag actttggttt
    52861 ttagggcaga cacatactga gccaaagact ttaagtcttc ttgccctttt tctaaaaagt
    52921 attcactact ggtcaagaga ggttggtgca aggtctcaaa agcaacttcc catttccaaa
    52981 ggcaaaagag ccagtagcca ccttgtccca aagaaagggc tagaagaaag agaccgatgc
    53041 ctttactgat ccaagttaat accatccctg caatcagaat gaggctaaca cttagattga
    53101 ctagccaaaa ttgaaggtag cgtttcatct ataactcctt gaacttataa ccataacccc
    53161 gaatggttcg aataaattga ggggctttag gattgtcttc aattttttcc ctcaacttac
    53221 caatatgaac gtccaccaaa cgtgtttcct gcccaaagtc atacccccag atacgttcca
    53281 aaagacgctc tctagtcagt gtcatgttgg gatgtttcat aagatagagc aagagttcaa
    53341 attcttttgg ggtcaaactc agtaacttat tcgccttgta gacttcatga cgctcagggt
    53401 atactttcaa ggtcccaaat agccaagaat cgtcagcgat attatctgaa tcatctcctt
    53461 cttgttctcc tttagttcgc ctgaggacag ccttgacacg cgccagcaat tctctagggc
    53521 taaaaggttt ggtcaggtag tcatcagccc ctaattccaa ggccaaaacc ttatcaaatt
    53581 catcactttt cgcagaaacc atcataattg gagttttgac gcctttggct ctcagccgct
    53641 tacaaacttc catgccatct aattgtggta acatgatatc aagcaagata aaatcaaagg
    53701 gttctgtttc tgccaaagct aaggccttcc gtccatttgt taccaattga gtagaaaagc
    53761 cttccttact taaatggtag tcaagcaatt tcagaatgtg ttcttcatca tccactaata
    53821 agacttgttt tgtcatctat tatctcctat tggtaacatt ataacacaat tatcagaaat
    53881 cctaacattg ctaaatcaga ttaaatttgc ttatcaagac tagtatctgg tcaaacgctc
    53941 aatcatctcc ttgtgctctg gataggtcgc cagtagatct accctttcaa ataattcaaa
    54001 atcctcaaat tcaaaaccag gagcaacaag acaagaaacc agagcatcat ccttatcaac
    54061 tgttgatccc caaatagtgc ccttaggaac acagtagtga agttgttgcc ctttggatat
    54121 gtccaggcct aaagtgactg cttcgtagtg accatctgct gtaatcatgt gaacagtaag
    54181 tggggattct gcatgaaaat accagatttc atctgctgtc aatcggtgaa aatgtgaagg
    54241 attcgtttct tcttaataag aaataaatac tggtataaag cgcccttccc ttaccagcaa
    54301 ggtttatagt gtctgaagct ttttttgttt gtctaaaata gccaccttca acatggggag
    54361 ctaactctag agttcttatc aagtcttctt tatccgtcgg agccaatggg ttgaagtaac
    54421 tcttgttcaa agtggtgtta cgatttcaag aactcctctc agttctgagg acacggtaat
    54481 gattgatgcg acggaagtac aaatcaatcg ccctaaaaaa agaattagcg aatcattctg
    54541 gtaaaaagaa atttcacgct atgaaggctc aagcgattgt cacaagtcaa gggagaattg
    54601 tttctttgga tatcgctgtg aactatagtc atgatatgaa gttgttcaaa atgagtcgca
    54661 gaaatatcgg acaagctggt aaaatcttgg ctgacagtgg ttatcaaggg ctcatgaaga
    54721 tatatcctca agcacaaatt ccacgtaaat ccagcaaact caagccgcta acagttgaag
    54781 ataaagcctg taaccatgcg ctatctaagg agagaagcaa ggttgagaac atctttgcca
    54841 aagtaaaaac gtttaaaatg ttttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat
    54901 tacgaatgaa tttgattgct ggtattatca atcatgaact aggattctag ttttgcagga
    54961 agtctattat ttggttaggt gaattagtga agcgtttagg caagtgtctc tggttacgac
    55021 gtcatggcct ctaaatcgat tatatttagg ggtcatgact agtgaagcag ttagctagtt
    55081 cgcatataag cggctagcgt ctaacaatta ggaactttag ttccaataac tttaagatta
    55141 cgacgtttta ggacataaat cgatcatatt tatgtcctaa aactagtgaa gcgcctagcc
    55201 aaagtccgaa taggatttgg cgttagttac ttagattgct ttgcaatcaa gtaactttgg
    55261 cgatttacat cttctctggt gcttctactc caagcaagcg aagggcttct ttgagaacga
    55321 ctgcggttgc gtagctgagg gctagacggc tgtcgcgttc tgggctttca tccaagatac
    55381 gtgtatgtgc atagtatttg ttaaaggatt gagccaggct aattgcaaat ttagcaatga
    55441 tagaaggttc aaagttatct gccgcacggt tgataatacg tgggaagtct tgaatgagtt
    55501 taatgatttc ccagctttca gtatcattca agctatagtt gccagctgtt tctggtttga
    55561 aatcggcttt gcgtaagata gattggatac gagcgtaggc atattgaacg taaggtccag
    55621 tttcaccctc gaaggatacc atagcttcta ggtcgaagtc gtatccattt gtacggtcag
    55681 ttttgaggtc atagaattta atggctccaa ccccaacagc atgtgctact tggtctttgt
    55741 tttctagttc aggattttta gcctcgattt ggaccttggc acggctaaca gcctctgcaa
    55801 cagtaggctc tagcaagatg acattccctt tacgagtaga gagcttcttc ccttcttttg
    55861 taaccagacc aaaaggaacg tgggtaatgt cgtcactcca atcgtagccc atctcttgca
    55921 agacagcttt gagttgttta aagtgggcag attgctcttg accaacgacg tagatagatt
    55981 tagcaaattg gtattcattt ttacggtaga gggctgcagc caagtcacgt gtgatataga
    56041 gagttgcacc atcagacttc ttgatgaggg ctggatgttc aattccatat ttctcaagat
    56101 tcacaacttg ggcaccttct gattcaagaa gtagtccttt ttcagaaaga atgtctacaa
    56161 ctgcatccat cttatcattg tagaaggctt ctccgttata gctgtcaaat tcaaccttca
    56221 attcattgta aaggcggtta aattccacta aactttcatc gcggaaccat tgccaaagag
    56281 cgagagcttc ctcatctcca ttttcaagtt tacggaacca ttcgcgcgct tcttcatcca
    56341 agctagggtc attttcagct tcagcgttga tgcggacata gagtttaagg agttcatcga
    56401 ttggatgagc ttttacagct tcttcgtcgc cccatttttt ataggcaaca atcaacatcc
    56461 caaattgttt accccagtct cccaaatggt tgaccttgac cgtttgataa ccgatttttt
    56521 ggaaaatatg tgacaagcta tctccgataa cagttgaacg caggtggcca atagaaaatg
    56581 gtttagcgat attcggacta gacatgtcga taacaacatt ttcttgttta ccaatatttt
    56641 ggtcagcata gtgttctttt tcagtggtaa cagcttgcaa tacttgagca gaaatggcag
    56701 atttatcaag gaaaaagtta acgtaaggtc ctgttgcgac aactttttca aaggcttggc
    56761 tgttcatttt ttcagccagt tcagccgcaa tcatttgtgg tgctttacgt tcgacttttg
    56821 caagagaaaa agcagggaaa gcaatgtctc ccatttctga gtttttaggg gtttccagta
    56881 actttaaaac agcctcttgg tccaggctat caatgatgct agataattcg ctagcaatca
    56941 attcttttgt attcattaag agctcctttt tggacttttc tactatttta tcacaatttt
    57001 aaagaaagaa gaaaaaattt ttgaaatctc ctgttttttt ggtataatat ggttataaat
    57061 atagttataa atatgcacgc aagaggattt tatgagaaaa agagatcgtc atcagttaat
    57121 aaaaaaaatg attactgagg agaaattaag tacacaaaaa gaaattcaag atcggttgga
    57181 ggcgcacaat gtttgtgtga cgcagacaac cttgtctcgt gatttgcgcg aaatcggctt
    57241 gaccaaggtc aagaaaaatg atatggtgta ttatgtacta gtaaatgaga cagaaaagat
    57301 tgatttggtg gaatttttgt ctcatcattt agaaggtgtt gcaagagcag agtttacctt
    57361 ggtgcttcat accaaattgg gagaagcctc tgttttggca aatattgtag atgtaaacaa
    57421 ggatgaatgg attttaggaa cagttgctgg tgccaatacc ttattggtta tttgtcgaga
    57481 tcagcacgtt gccaaactca tggaagatcg tttgctagat ttgatgaaag ataagtaagg
    57541 tcttgggagt tgctctcaag acttattttt gaaaaggaga gacagaaaat ggcgatagaa
    57601 aagctatcac ccggcatgca acagtatgtg gatattaaaa agcaatatcc agatgctttt
    57661 ttgctctttc ggatgggtga tttttatgaa ttattttatg aggatgcggt caatgctgcg
    57721 cagattctgg aaatttcctt aacgagtcgc aacaagaatg ccgacaatcc gatccctatg
    57781 gcgggtgttc cctatcattc tgcccaacag tatatcgatg tcttgattga gcagggttat
    57841 aaggtggcta tcgcagagca gatggaagat cctaaacaag cagttggggt tgttaaacga
    57901 gaggttgttc aggtcattac gccagggaca gtggtcgata gcagtaagcc ggacagtcag
    57961 aataattttt tggtttccat agactgcgaa ggcaatcaat ttggcctagc ttatatggat
    58021 ttggtgacgg gtgactttta tgtgacaggt cttttggatt tcacgctggt ttgtggggaa
    58081 atccgtaacc tcaaggctcg agaagtggtg ttgggttatg acttgtctga ggaagaagaa
    58141 caaatcctca gccgccagat gaatctggta ctctcttatg aaaaagaaag ctttgaagac
    58201 cttcatttat tggatttgcg attggcaacg gtggagcaaa cggcatctag taagctgctc
    58261 cagtatgttc atcggactca gatgagggaa ttgaaccacc tcaaacctgt tatccgctac
    58321 gaaattaagg atttcttgca gatggattat gcgaccaagg ctagtctgga tttggttgag
    58381 aatgctcgct caggtaagaa acaaggcagt cttttctggc ttttggatga aaccaaaacg
    58441 gctatgggga tgcgtctctt gcgttcttgg attcatcgcc ccttgattga taaggaacga
    58501 atcgtccaac gtcaagaagt agtgcaggtc tttctcgacc atttctttga gcgtagtgac
    58561 ttgacagaca gtctcaaggg tgtttatgac attgagcgct tggctagtcg tgtttctttt
    58621 ggcaaaacca atccaaagga tctcttgcag ttggcgacta ccttgtctag tgtgccacgg
    58681 attcgtgcga ttttagaagg gatggagcaa cctactctag cctatctcat cgcacaactg
    58741 gatgcaatcc ctgagttgga gagtttgatt agcgcagcga ttgctcctga agctcctcat
    58801 gtgattacag atgggggaat tatccggact ggatttgatg agactttaga caagtatcgt
    58861 tgcgttctca gagaagggac tagctggatt gctgagattg aggctaagga gcgagaaaac
    58921 tctggtatca gcacgctcaa gattgactac aataaaaagg atggctacta ttttcatgtg
    58981 accaattcgc aactaggaaa tgtgccagcc cactttttcc gcaaggcgac gctgaaaaac
    59041 tcagaacgct ttggaaccga agaattagcc cgtatcgagg gagatatgct tgaggcgcgt
    59101 gagaagtcag ccaacctcga atacgaaata tttatgcgca ttcgtgaaga ggtcggcaag
    59161 tacatccagc gtttacaagc tctagcccaa ggaattgcga cggttgatgt cttacagagt
    59221 ctggcggttg tggctgaaac ccagcatttg attcgacctg agtttggtga cgattcacaa
    59281 attgatatcc ggaaagggcg ccatgctgtc gttgaaaagg ttatgggggc tcagacctat
    59341 attccaaata cgattcagat ggcagaagat accagtattc aactggttac agggccaaac
    59401 atgagtggga agtctaccta tatgcgtcag ttagccatga cggcggttat ggcccagctg
    59461 ggttcctatg ttcctgctga aagcgcccat ttaccgattt ttgatgcgat ttttacccgt
    59521 atcggagcag cagatgactt ggtttcgggt cagtcaacct ttatggtgga gatgatggag
    59581 gccaataatg ccatttcgca tgcgaccaag aactctctca ttctctttga tgaattggga
    59641 cgtggaactg caacttatga cgggatggct cttgctcagt ccatcatcga atatatccat
    59701 gagcacatcg gagctaagac cctctttgcg acccactacc atgagttgac tagtctggag
    59761 tctagtttac aacacttggt caatgtccac gtggcaactt tggagcagga tgggcaggtc
    59821 accttccttc acaagattga accgggacca gctgataaat cctacggtat ccatgttgcc
    59881 aagattgctg gcttgccagc agacctttta gcaagggcgg ataagatttt gactcagcta
    59941 gagaatcaag gaacagagag tcctcctccc atgagacaaa ctagtgctgt cactgaacag
    60001 atttcactct ttgatagggc agaagagcat cctatcctag cagaattagc taaactggat
    60061 gtgtataata tgacacctat gcaggttatg aatgtcttag tagagttaaa acagaaacta
    60121 taaaaccaag actcactagt taatctagct gtatcaagga gacttctttg acaattctcc
    60181 acttttttgc tagaataaca tcacacaaac agaatgaaaa ggagctgacg cattgtcgct
    60241 cccttttgtc tattttttaa ggagaaagta tgctgattca gaaaataaaa acctacaagt
    60301 ggcaggcctt ggcttcgctc ctgatgacag gcttgatggt tgctagttca cttctgcaac
    60361 cgcgttatct gcaggaagta ttagacgccc tccttactgg gaaatatgaa gctatttata
    60421 gtatcggggc ttggttgatt ggtgtggccg tagtcggtct agttgctggt ggactcaatg
    60481 ttgtcctcgc agcctatatt gcccaaggag tttcatccga ccttcgggag gatgccttcc
    60541 gtaaaattca aaccttttct tatgctgata ttgaacaatt taatgcggga aatctagtcg
    60601 ttcgaatgac aaatgatatc aaccagattc agaacgttgt catgatgacc ttccaaattc
    60661 ttttcagact tcccctcttg ttcatcggtt cgtttatcct agcggttcaa accttacctt
    60721 ctctgtggtg ggtgattgtt ctcatggtag tcttgatttt tggtttgact gctgtcatga
    60781 tgggaatgat ggggcctcgt tttgccaagt ttcaaaccct tcttgagcgc atcaatgcca
    60841 ttgccaagga aaatttacgt ggcgttcgtg tggtcaagtc ctttgtccaa gaaaaagagc
    60901 aatttgctaa gtttacagag gtctcagacg agctttttgg tcaaaacctt tacattggtt
    60961 atgccttttc agtagtggaa ccctttatga tgttggttgg ttacggggcg gtcttcctct
    61021 ctatttggct ggtcgcggga atggttcagt cggatccgtc tgttgttggt tccatcgctt
    61081 cttttgttaa ttacctaagc cagattatct ttaccattgt tatggttgga tttttgggaa
    61141 attctgtcag ccgtgccatg atttccatgc gtcgtattcg agaaattctt gacgcagagc
    61201 cagctatgac cttcaaggat atcccagatg aagagttggt tggaagcctt agctttgaaa
    61261 atgtgacctt tacctatcca atggacaagg aaccgatgct gaaagatgtg agctttacta
    61321 ttgaacctgg tcaaatggtt ggtgtagttg gagcgactgg tgcaggaaag tcaaccttgg
    61381 ctcaattgat tccacgtctc tttgatccac agaacggggc cattaaaatc ggtggcaagg
    61441 atattcgaga agtgagtgaa ggaaccctgc gtaaaacagt ttccatcgtt ctccaacgtg
    61501 ccattctttt tagtggaacg attgcagata acttgagaca ggggaaggga aatgctactc
    61561 tatttgaaat ggagcgcgca gccaatattg cccaggctag tgaattcatt catcgtatgg
    61621 agaaaacctt tgaaagtcca gttgaagaac ggggaaccaa tttctctggt ggacaaaaac
    61681 aaaggatgtc gattgcgcgt gggattgtca gcaatccacg tattctgatt tttgatgatt
    61741 cgacctcagc cttggatgcc aaatcagagc gcttggtgca agaagctttg aataaggact
    61801 tgaaggggac gacaaccatt attattgctc aaaaaattag ctcggttgtc catgcagaca
    61861 agatcttggt tctaaatcaa ggacgattga ttggtcaagg tacgcatgca gacttggttg
    61921 ccaacaatgc cgtttaccgt gaaatctatg aaacacagaa atgaaagaca aactataaga
    61981 aaagtcaata gttttatcta aactatttct tatttcaatt tgatgatttg gcgatgattt
    62041 tagagcacgg caaaaggccc ttgaaaaagt ccattttttc aaaggtaatc ctgtgttaat
    62101 ttcagaaatt acatcacttt ttgttcgtca aatggcagct ctttttttag gatataaaac
    62161 agggttcgga taagtttttt tgcaaggtgg atgatggcta cattgtaatg ttttccttgt
    62221 tctaatttag tcttaagata ggccttaaaa gcaggcgaaa agcgagggca tgctttggca
    62281 gcttgtatga gtacctaccg cagatgaggg gaactccgtt tgaccattct tcctgctaaa
    62341 tcaatctgat ctgactgata aatagaagaa tccagtccag cgaaagcttg taattgagca
    62401 ggattatcaa aggcatgaat atttcgaatc tcagctaaaa tgaccgcccc taaacgatcc
    62461 ccaatcccag taaccgtcgt gatgaccgag ttgaactcag ccatcaagtc attgacacat
    62521 gtttccgcct tgtcaatgag ccccttgtaa tgtttgatgt tttcattaca cgagataaaa
    62581 cgtctatgcg ttatcaaact cattaccaat taaaacaaaa agctgtggtt agatcctttc
    62641 ggaaattgtc aagcgattgg aggaaatgaa ctaatccaca gcggcttatt ccaagtatac
    62701 cacttgggct ttggcagtag ctaactgcgc taaatataat ataaggagga gtaaaatgaa
    62761 gacagttcaa tttttttggc attattttaa ggtctacaag ttctcatttg tagttgtcat
    62821 cctgatgatt gttctggcga cttttgccca agccctcttt ccagtctttt ctggacaagc
    62881 ggtgacgcag ctagccaatt tagttcaagc tcatcaaaat ggcaatccag aacttgtatg
    62941 gcaaagccta tcaggaatca tggtcaatct tggcctgctg gttttggttc tatttatctc
    63001 tagtgtaata tacatgtgtc tcatgacgcg cgtgattgca gaatcgacca acgagatgcg
    63061 caaaggtctc tttggtaagc ttgctcagtt gacggtttct ttctttgacc gtcgacaaga
    63121 tggcgatatc ctgtctcatt ttaccagtga tttggataat atcctccaag cctttaacga
    63181 aagcttgatt caggtcatga gcaatattgt tttatacatt ggtctgattc ttgtcatgtt
    63241 ttcgagaaat gtgacgctgg ctctcatcac cattgccagc accccattgg ctttccttat
    63301 gctgattttc atcgtgaaaa tggcacgcaa atacaccaac ctccagcaga aagaggtagg
    63361 gaagctcaac gcctatatgg atgagagcat ttcaggccaa aaagccgtga ttgtgcaagg
    63421 aattcaagag gatatgatgg caggatttct tgaacaaaat gagcgcgtgc gcaaggcaac
    63481 ctttaaagga agaatgttct caggaattct tttccctatc atgaatggga tgagcctgat
    63541 taatacagcc atcgtcatct ttgctggttc ggctgtactt ttgaatgata agtctattga
    63601 aacaagtaca gccctaggtt tgattgttat gtttgcacaa ttttcacagc agtactacca
    63661 gcctattatc caagttgcag cgagttgggg aagccttcag ttggccttta ctggagctga
    63721 acgaattcag gaaatgtttg atgcagagga ggaaatccga cctgaaaagg ctccaacctt
    63781 cactaagttg caagaaagtg ttgaaatcag tcatatcgat ttttcatact tgcctgataa
    63841 acctattttg aaagatgtca gcatttctgc ccctaaaggc cagatgacag cagttgttgg
    63901 gccgacaggt tcaggaaaaa cgactattat gaacctcatc aatcgctttt atgatgttga
    63961 tgctggtggt atttattttg atggtaaaga cattcgtggc tatgacttag atagtcttag
    64021 aagcaaggtg ggaattgtat tgcaagattc ggtcttgttt agcggaacga ttcgagacaa
    64081 tatccgattt ggtgtgccag atgctagtca ggaaatggtt gaggtagcag caaaagcaac
    64141 ccacattcac gactatatcg aaagtttgcc tgataagtac gatactctta ttgatgatga
    64201 ccagagcatc ttttcaacag ggcagaagca attgatttca atcgctcgaa ccctgatgac
    64261 agatccagaa gttctcattc tcgatgaagc aacttcaaac gtagatacgg tgacagaaag
    64321 caagattcag catgccatgg aggtggttgt agcaggtaga actagtttcg tcattgccca
    64381 ccgcttgaaa accattctca atgcagatca gattattgtc cttaaagatg gagaagtcat
    64441 tgaacgtggt aaccaccatg aacttttgaa gctaggtggc ttttattcag aactctatca
    64501 caatcaattt gttttcgaat aagaaagaag ttgtcctatg tgggcagctt tttcttgtcc
    64561 ataaaaaatg tttatcacag ccttaaaaaa aacatattag acgaaagtca ttttgagtga
    64621 tatgatagga ctatcgttag cattcgaaag gagaggcatc atggctagaa cggttgtagg
    64681 agttgctgca aatctatgtc ccgtagacgc agaaggcaaa atcattcatt catctgtatc
    64741 ttgtagattc gcagagatca ttcgtcaagt cggtggtctc cctttagtca ttcctgttgg
    64801 tgatgagtca gttgtacgtg attatgtgga aatgattgac aaactcattt tgacaggagg
    64861 ccaaaatgtt catcctcagt tttatggaga gaagaagacc gtcgagagcg atgattacaa
    64921 tctggtccgt gacgaatttg aattggcact cttgaaggaa gcgcttcgtc agaataaacc
    64981 aattatggca atctgtcgcg gtgtccaact tgtcaatgtt gcctttggtg gaaccctcaa
    65041 tcaagaaatc gaaggtcact ggcaaggact acctttcggg acatctcact ctattgagac
    65101 agtagaagga agcgtggtgg ctaagctatt tgggaaagaa agtcaggtca actcagtcca
    65161 tcgtcaaagc attaaagatt tggcacctaa tttccgtgta actgctattg attcaagaga
    65221 ccaaactatt gaagcgattg agtctatcga cgagcaccgc attatcggtt tacagtggca
    65281 tccagagttt ctggttaatg aagaagatgg caatttagaa ttatttgagt atttattgaa
    65341 tgaactgtaa cgattggaac atctggtcgt tctttctttt attttttcaa aatttttaga
    65401 atgtggcatt ttacgcaaac gtttgaattc tgataaaaat tggagaaatt cgacaaaaaa
    65461 acttgaaaaa aacgaaggta agcgttatga tagaaaagaa gaaatattgg aggaagaaca
    65521 tgtcacatat taaatttgat tattcaaaag ttttagacaa atttgttgca ccacatgaag
    65581 tggaatacat gcaatcacaa gtaacagcag cagatgaatt gatccgtaaa ggaactggtg
    65641 ctggtagcga cttcttggga tggttggacc ttcctgaaaa atacgaccgc gaagaatttg
    65701 accgcatctt gaaagctgct gagcaaatca agtcagacag cgatgttttg gttgtgatcg
    65761 gtattggtgg atcttacctt ggtgcaaaag cagcaatcga cttcttgaat caccattttg
    65821 ctaacttgca aacaaaagaa gaacgcaaag ctccacaaat cctttacgca ggaaactcaa
    65881 tctcatctac ttaccttgct gacttggtag agtatgttgc agacaaagac ttctcagtaa
    65941 acgtaatttc taaatcaggt acaacaactg aaccagcgat tgctttccgt gtctttaaag
    66001 aactcttggt taagaaatac ggtcaagaag aagctaacaa acgtatctat gcaacaactg
    66061 accgccaaaa gggtgctgtt aaggttgaag cagacgctaa cggttgggga acatttgttg
    66121 ttccagatga tatcggtgga cgcttctcag tattgacagc cgttggtttg cttccaatcg
    66181 cagcatcagg agctgacata aaagctctta tggaaggtgc gaatgcagct cgcaaagact
    66241 acacttcaga caaaatctct gaaaacgaag cttaccaata cgcagtcgtt cgtaacatcc
    66301 tttatcgtaa aggctatgca actgagatct tggtaaacta tgagccatca cttcaatact
    66361 tctcagaatg gtggaaacaa ttggctggtg aatcagaagg aaaagaccaa aaaggtatct
    66421 acccaacttc agccaacttc tcaactgact tgcactcact tggtcaattt atccaagaag
    66481 gaactcgtat catgtttgaa acagttgtcc gtgttgacaa acctcgtaaa aacgtgctta
    66541 ttcctacttt ggaagaagac cttgacggac ttggttacct tcaaggaaaa gacgttgact
    66601 ttgtaaacaa aaaagcaact gacggtgttc ttcttgccca cacagatggt gatgtaccaa
    66661 acatgtatgt gactcttcca gagcaagacg ctttcactct tggttacact atctacttct
    66721 tcgaattggc aattgccctt tcaggttact tgaatgctat caacccattt gaccaaccag
    66781 gtgttgaagc ttataaacgt aacatgtttg cccttcttgg aaaaccagga tttgaagaat
    66841 tgagcaaaga acttaacgca cgtctataat agaagaaaag agtggtttgc ccactctttt
    66901 tactctcttt atccatagaa attggactca gccaagactt gtgatataat atagaaagca
    66961 aaaaggcaga cgcctagata ataggagaaa ctatgtcaaa agatatccgc gtacgttacg
    67021 caccaagtcc aacaggacta ctacacatcg gaaatgcccg tacagcattg tttaactact
    67081 tgtatgcgcg ccatcatggt ggaacatttc tcatccgtat cgaagatact gaccgtaaac
    67141 gccatgtcga ggatggtgaa cgttcacaac ttgaaaacct tcgctggtta ggcatggatt
    67201 gggatgaaag tccagaatca catgagaatt atcgccagtc tgagcgtttg gacttgtatc
    67261 aaaaatacat cgaccaacta ttagctgaag gaaaagccta caaatcttac gttacagaag
    67321 aagagttggc agctgaacgt gaacgtcaag aagtagctgg cgaaacacca cgctacatca
    67381 atgaatacct tggtatgagt gaagaagaaa aagcagctta catcgcagaa cgtgaagcag
    67441 cagggatcat cccaactgtt cgtttggctg tcaatgagtc aggtatctac aagtggcatg
    67501 atatggtcaa aggcgatatc gaatttgaag gtggcaatat cggtggtgac tgggttatcc
    67561 aaaagaaaga cggttaccca acttacaact ttgccgttgt tatcgatgac cacgatatgc
    67621 aaatctctca tgttatccgt ggagatgacc atattgctaa tacaccaaaa cagcttatgg
    67681 tctatgaagc tcttggttgg gaagctccag agttcggtca catgaccttg attatcaact
    67741 ctgagactgg taagaagtta tctaaacgtg atactaatac acttcaattt atcgaagatt
    67801 accgaaagaa aggttacctt ccagaagcag tctttaactt tattgctctt cttggttgga
    67861 acccaggtgg tgaagatgag attttctctc gtgaagaact cattaaactt tttgatgaaa
    67921 accgcctcag caagtcacca gcagcctttg atcagaaaaa actagactgg atgagcaatg
    67981 attatatcaa gaatgcagac cttgaaacca tctttgaaat ggcaaaacca ttcttagagg
    68041 aagcaggccg tttgactgac aaggctgaaa aattagttga gctctataaa ccacaaatga
    68101 aatcagtaga tgagattatc ccattgacag atcttttctt ctcagatttc ccagaattga
    68161 cagaagcaga gcgcgaagtc atgacgggtg aaacagttcc aacagttctt gaagcattca
    68221 aagcaaaact tgaagcgatg acagatgata aatttgtgac agaaaatatc ttcccacaaa
    68281 ttaaagcagt tcaaaaagaa acaggtatta aagggaaaaa tcttttcatg cctattcgta
    68341 tcgcagtttc aggcgaaatg catgggccag aattaccaga tacaattttc ttgcttggac
    68401 gtgaaaaatc aattcagcat atcgaaaaca tgctaaaaga aatctctaaa taaagaggat
    68461 acaataatgg acatctggga aaagatgtac gaagaagcac agaaactata caatccacat
    68521 gaagtatcag actttgttta tgccaatcat gttgtagctg cagtagaagc agaagatgga
    68581 caaatattta caggattctg tatggaggga acctgtggtg ttttccatct ctgcgcagaa
    68641 cgggcgaccc tcttcaatat gtaccaattt tcaggacaaa ctaaggttaa gaaagtatta
    68701 gcctttcgag acaaaccacc ttatggtgga agttcagcca tgccttgcgg tgcttgtaga
    68761 gaattccttt tagagcttaa tgctgaaaat aaagatgcag aattcatgat ggactataat
    68821 ataagaaaaa cagttaaagt cgcagaacta atcccttatt ggtggggaga agaacgtgct
    68881 tctaagttta atgagcgata gaagagtcag tataattctg gcttttttta ctataagttg
    68941 aaaaaat
//
LOCUS       DAVKQB010000003        66513 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKQB010000003 DAVKQB010000000
VERSION     DAVKQB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 66513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.003"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(166..1914)
                     /locus_tag="VW528_000146"
     CDS             complement(166..1914)
                     /locus_tag="VW528_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911038.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLVIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(1916..3640)
                     /locus_tag="VW528_000147"
     CDS             complement(1916..3640)
                     /locus_tag="VW528_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911039.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLSKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQKLWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFIILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(3698..4636)
                     /gene="msrB"
                     /locus_tag="VW528_000148"
     CDS             complement(3698..4636)
                     /gene="msrB"
                     /locus_tag="VW528_000148"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9911040.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            complement(4784..4855)
                     /locus_tag="VW528_000149"
                     /pseudo
     CDS             complement(4784..4855)
                     /locus_tag="VW528_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4931..5800)
                     /locus_tag="VW528_000150"
     CDS             complement(4931..5800)
                     /locus_tag="VW528_000150"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9911041.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAAAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            complement(5802..7088)
                     /locus_tag="VW528_000151"
     CDS             complement(5802..7088)
                     /locus_tag="VW528_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9911042.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(7239..7976)
                     /gene="mecA"
                     /locus_tag="VW528_000152"
     CDS             complement(7239..7976)
                     /gene="mecA"
                     /locus_tag="VW528_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9911043.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(8176..9210)
                     /locus_tag="VW528_000153"
     CDS             complement(8176..9210)
                     /locus_tag="VW528_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9911044.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(9239..10675)
                     /locus_tag="VW528_000154"
     CDS             complement(9239..10675)
                     /locus_tag="VW528_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9911045.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNNVSLLSTLLFLL
                     VSSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVM
                     VSILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRL
                     FERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSF
                     IAANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAM
                     GVLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPV
                     LDYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYI
                     MELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSE
                     SFFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            complement(10691..11677)
                     /locus_tag="VW528_000155"
     CDS             complement(10691..11677)
                     /locus_tag="VW528_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9911046.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(11679..12782)
                     /locus_tag="VW528_000156"
     CDS             complement(11679..12782)
                     /locus_tag="VW528_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9911047.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(12772..13617)
                     /locus_tag="VW528_000157"
     CDS             complement(12772..13617)
                     /locus_tag="VW528_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9911048.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKDPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(13896..15170)
                     /gene="psr"
                     /locus_tag="VW528_000158"
     CDS             complement(13896..15170)
                     /gene="psr"
                     /locus_tag="VW528_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9911049.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(15167..16015)
                     /gene="pheA"
                     /locus_tag="VW528_000159"
     CDS             complement(15167..16015)
                     /gene="pheA"
                     /locus_tag="VW528_000159"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9911050.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEGVTIPLQSQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(16012..16488)
                     /locus_tag="VW528_000160"
     CDS             complement(16012..16488)
                     /locus_tag="VW528_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9911051.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(16481..17764)
                     /gene="aroA"
                     /locus_tag="VW528_000161"
     CDS             complement(16481..17764)
                     /gene="aroA"
                     /locus_tag="VW528_000161"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9911052.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSASFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(17856..18194)
                     /locus_tag="VW528_000162"
     CDS             complement(17856..18194)
                     /locus_tag="VW528_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9911053.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(18205..19308)
                     /locus_tag="VW528_000163"
     CDS             complement(18205..19308)
                     /locus_tag="VW528_000163"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9911054.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLESIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITKNTDYTVVIK"
     gene            complement(19318..20484)
                     /gene="aroC"
                     /locus_tag="VW528_000164"
     CDS             complement(19318..20484)
                     /gene="aroC"
                     /locus_tag="VW528_000164"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9911055.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(20494..21561)
                     /locus_tag="VW528_000165"
     CDS             complement(20494..21561)
                     /locus_tag="VW528_000165"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9911056.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            complement(21580..22434)
                     /locus_tag="VW528_000166"
     CDS             complement(21580..22434)
                     /locus_tag="VW528_000166"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9911057.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEAHLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            complement(22424..23101)
                     /locus_tag="VW528_000167"
     CDS             complement(22424..23101)
                     /locus_tag="VW528_000167"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9911058.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(23098..24261)
                     /locus_tag="VW528_000168"
     CDS             complement(23098..24261)
                     /locus_tag="VW528_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9911059.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(24946..26580)
                     /locus_tag="VW528_000169"
     CDS             complement(24946..26580)
                     /locus_tag="VW528_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911060.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMIICFSNIAIHFPFEEIPIYFSLMIAILLVIAFSTSLTAFYNVFYESK
                     DLISYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVDPFSTEAILGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFFPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(26561..27310)
                     /locus_tag="VW528_000170"
     CDS             complement(26561..27310)
                     /locus_tag="VW528_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911061.1"
                     /translation="MIEFQNVSKLYGDKKALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            27489..28943
                     /locus_tag="VW528_000171"
     CDS             27489..28943
                     /locus_tag="VW528_000171"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9911062.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(29033..31651)
                     /gene="alaS"
                     /locus_tag="VW528_000172"
     CDS             complement(29033..31651)
                     /gene="alaS"
                     /locus_tag="VW528_000172"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9911063.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(31673..32158)
                     /locus_tag="VW528_000173"
     CDS             complement(31673..32158)
                     /locus_tag="VW528_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9911064.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(32433..32672)
                     /locus_tag="VW528_000174"
     CDS             complement(32433..32672)
                     /locus_tag="VW528_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911065.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLNDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(32941..34011)
                     /locus_tag="VW528_000175"
     CDS             complement(32941..34011)
                     /locus_tag="VW528_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9911066.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGSEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(34008..34781)
                     /locus_tag="VW528_000176"
     CDS             complement(34008..34781)
                     /locus_tag="VW528_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9911067.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(34778..35584)
                     /locus_tag="VW528_000177"
     CDS             complement(34778..35584)
                     /locus_tag="VW528_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9911068.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(35565..36722)
                     /locus_tag="VW528_000178"
     CDS             complement(35565..36722)
                     /locus_tag="VW528_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911069.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(36883..37788)
                     /gene="murB"
                     /locus_tag="VW528_000179"
     CDS             complement(36883..37788)
                     /gene="murB"
                     /locus_tag="VW528_000179"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9911070.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(37900..38652)
                     /locus_tag="VW528_000180"
                     /pseudo
     CDS             complement(37900..38652)
                     /locus_tag="VW528_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
     gene            complement(38662..39393)
                     /gene="budA"
                     /locus_tag="VW528_000181"
     CDS             complement(38662..39393)
                     /gene="budA"
                     /locus_tag="VW528_000181"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9911071.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            complement(39877..40398)
                     /locus_tag="VW528_000182"
     CDS             complement(39877..40398)
                     /locus_tag="VW528_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcin A-M57"
                     /protein_id="HEU9911072.1"
                     /translation="MKFKKIVTSALVVLSLFSIMSPSIASVRVFADDVTTTEVSEIST
                     QEQKQIDDVANVLEQMFRNGVNEKNFTEYVYKNFSQKDIALAENELETNINNPYDRVP
                     WDEMGGCIAGKIRDEFFAMINVSLIVKYAQKKAWSELAKVVLRFVKANGLKTNIYIIA
                     GQLAIWAVQCGLE"
     gene            40829..42100
                     /locus_tag="VW528_000183"
     CDS             40829..42100
                     /locus_tag="VW528_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9911073.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(42120..42935)
                     /locus_tag="VW528_000184"
     CDS             complement(42120..42935)
                     /locus_tag="VW528_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9911074.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(43092..43745)
                     /gene="phoU"
                     /locus_tag="VW528_000185"
     CDS             complement(43092..43745)
                     /gene="phoU"
                     /locus_tag="VW528_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9911075.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(43757..44515)
                     /gene="pstB"
                     /locus_tag="VW528_000186"
     CDS             complement(43757..44515)
                     /gene="pstB"
                     /locus_tag="VW528_000186"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9911076.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLNPQHKETEDYITGKFG"
     gene            complement(44528..45331)
                     /gene="pstB"
                     /locus_tag="VW528_000187"
     CDS             complement(44528..45331)
                     /gene="pstB"
                     /locus_tag="VW528_000187"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9911077.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(45342..46226)
                     /gene="pstA"
                     /locus_tag="VW528_000188"
     CDS             complement(45342..46226)
                     /gene="pstA"
                     /locus_tag="VW528_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9911078.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(46216..47133)
                     /gene="pstC"
                     /locus_tag="VW528_000189"
     CDS             complement(46216..47133)
                     /gene="pstC"
                     /locus_tag="VW528_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9911079.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(47133..48011)
                     /gene="pstS"
                     /locus_tag="VW528_000190"
     CDS             complement(47133..48011)
                     /gene="pstS"
                     /locus_tag="VW528_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9911080.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(48585..49889)
                     /locus_tag="VW528_000191"
     CDS             complement(48585..49889)
                     /locus_tag="VW528_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9911081.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(49890..50663)
                     /locus_tag="VW528_000192"
     CDS             complement(49890..50663)
                     /locus_tag="VW528_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9911082.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGHLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(50653..50931)
                     /locus_tag="VW528_000193"
     CDS             complement(50653..50931)
                     /locus_tag="VW528_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9911083.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(50931..51332)
                     /locus_tag="VW528_000194"
     CDS             complement(50931..51332)
                     /locus_tag="VW528_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9911084.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(51542..52159)
                     /locus_tag="VW528_000195"
     CDS             complement(51542..52159)
                     /locus_tag="VW528_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9911085.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(52149..52922)
                     /locus_tag="VW528_000196"
     CDS             complement(52149..52922)
                     /locus_tag="VW528_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9911086.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(52933..53670)
                     /locus_tag="VW528_000197"
     CDS             complement(52933..53670)
                     /locus_tag="VW528_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9911087.1"
                     /translation="MCLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(53675..54805)
                     /locus_tag="VW528_000198"
     CDS             complement(53675..54805)
                     /locus_tag="VW528_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9911088.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(54889..55278)
                     /locus_tag="VW528_000199"
     CDS             complement(54889..55278)
                     /locus_tag="VW528_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9911089.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(55294..55677)
                     /locus_tag="VW528_000200"
     CDS             complement(55294..55677)
                     /locus_tag="VW528_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9911090.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(55678..56466)
                     /locus_tag="VW528_000201"
     CDS             complement(55678..56466)
                     /locus_tag="VW528_000201"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9911091.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFSLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(56459..57394)
                     /locus_tag="VW528_000202"
     CDS             complement(56459..57394)
                     /locus_tag="VW528_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9911092.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(57524..57700)
                     /locus_tag="VW528_000203"
     CDS             complement(57524..57700)
                     /locus_tag="VW528_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9911093.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(57840..58547)
                     /locus_tag="VW528_000204"
     CDS             complement(57840..58547)
                     /locus_tag="VW528_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9911094.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            58698..59726
                     /gene="queA"
                     /locus_tag="VW528_000205"
     CDS             58698..59726
                     /gene="queA"
                     /locus_tag="VW528_000205"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9911095.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKIVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(60387..60776)
                     /locus_tag="VW528_000206"
     CDS             complement(60387..60776)
                     /locus_tag="VW528_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9911096.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(60880..61521)
                     /locus_tag="VW528_000207"
     CDS             complement(60880..61521)
                     /locus_tag="VW528_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002871528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9911097.1"
                     /translation="MNTSFAHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRFEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKIEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKTLKQELLKKEPSPKFLSKKFQSKQNISMAQLLIKVKK"
     gene            complement(62235..62786)
                     /locus_tag="VW528_000208"
     CDS             complement(62235..62786)
                     /locus_tag="VW528_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9911098.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSTENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(63185..64009)
                     /gene="nadE"
                     /locus_tag="VW528_000209"
     CDS             complement(63185..64009)
                     /gene="nadE"
                     /locus_tag="VW528_000209"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9911099.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(64006..65466)
                     /locus_tag="VW528_000210"
     CDS             complement(64006..65466)
                     /locus_tag="VW528_000210"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9911100.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIYNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFPEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DENGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(65582..66070)
                     /locus_tag="VW528_000211"
     CDS             complement(65582..66070)
                     /locus_tag="VW528_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911101.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(66060..66269)
                     /locus_tag="VW528_000212"
     CDS             complement(66060..66269)
                     /locus_tag="VW528_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9911102.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT        19800 a        15048 c        12397 g        19268 t
ORIGIN      
        1 ttatctacaa cctcaaagct gtgctttgag caacctacgg ctagcttcct agtttgctct
       61 ttgattttca ttgagtatta cagtgaacaa tttttctagc tattgtaaca aaaaagtcac
      121 tcgaaggcaa cttttttcgt ccataaactt tcaaaagaga atggcttatt cagaactaaa
      181 agccgcagct ttttgcattt ggtaatactt accctttcta tccatgagtt cttgatggtt
      241 accatattca acaatatcac catctactaa gacaagaatt aaatccgcat cctgaatggt
      301 tgacaaacgg tgagcaatga tgaagcttgt gcggcccttc atgagttttg caaaggcatc
      361 ctgtaccagc acttctgtcc gtgtatcaat ggaagaagtt gcctcgtcta agataagaat
      421 ctttggaata accagaaaga ctcgggctat ggtcaagagc tgagcttggc cgacagagag
      481 agattctcca gcattttcca acttggtatc gtatccctgt ggcaactgtt ggatgaaaaa
      541 gtctgcatta gctgctttgg cagcagcaat tacttgctct cgactggctt caggattgcc
      601 aaaggcaata ttatcatgaa tggtcccttg tgtgagccag gtttcttgaa gcaccatacc
      661 aaactgctgt ctcaatgata ctcgtgtata atcataaatg gattgcccat ccagcaagat
      721 atctcccgag ctaatgggat aaaaacgcat aaggagattg ataagagttg attttccagc
      781 acctgtcgga ccaacgatgg ctatcttact accagctgga atatcgatag acaagtcctt
      841 aatcaaaatt ttttcaggat ggtagccaaa agagacatgt ttaaaggaaa tagctccctt
      901 aacttggtca ctggtcaaga cttccttacc tgtttcagcc acttcagggc tatctaagac
      961 tccatagata cgctctacgc aagccagagc actttgcaac tcagctagca ctgaagaaat
     1021 atcattaaag ggcttggtgt attgctgaac atagttcaaa aaagtcacta aacgaccgac
     1081 ggtcaaggtt gaacccatca tgatacgata agctcctact ccagctaaaa gggcataaat
     1141 gagtgcattt acaaagcgag tcgaaggatt gaccgttgaa gaataaaaga tggctgactg
     1201 agaatagcct gagtagttgt catgagcctc acgcaatctt tggataaatt ctgtttgagc
     1261 attgaaggac tggattatag tctgctggct aagcgattct tcaatcaact gagtctgaat
     1321 tcccctcgtc tctgtttgct tctggaagag atgataggat ctcttggcaa taaagcgtga
     1381 aatcaccatg gacagtggcg tcaacagcaa gactaagaga gtcatgagga gatgaatttg
     1441 gagcatggct agaatactaa ccaaaatcat caaaacacca atgaaaaatt ggttaaaaat
     1501 catggtcaag ccagctgcca actgttcgat gtccgtggtt acacgactaa ccatctctcc
     1561 actaccttgc ctatctacaa aagcaatcgg taaacgatgg agcttatgga tgattcgctc
     1621 tcgcaaatct ctagtataag agaagattag acgattatag aggagaggat tggcccattg
     1681 taccagagta tttcctatta ccaccaagag catctggaga aaaatctgcc aaaaaactgg
     1741 tgatgaacca gccactagga cttggtcaat gacctgccca atcagaatag gtaggtaaat
     1801 tgataagcca acttgggcaa tagttcctag aaaggctagg aaaaggagga agggatggtt
     1861 tgctaaatct acggctaaac gtttgagcgt ctggtttaca gtttgtcgtt tcattctagt
     1921 cctcctttcc atgttgggat gcattgattt cacgatagac ttggctggat ttcatcaagt
     1981 catcgtgctt gccaacagct agcaactcac ctttttccaa gaggagaatc tggtccgcca
     2041 tctgtaaagt tgaggttcgt tgagagatca aaattaagct cgtgtttgga aaattttctc
     2101 taatagcttt caagagcttg gactctgtaa tggtatccag tgccgaggtt gcatcatcta
     2161 ggatgataaa cggagcttgg cgcaagactg ctcgggcgat agacaacctt tgtttttgtc
     2221 cacctgagaa atttcgcccc cctgcctcaa ctagggcatc caaaagtcct tccttatcac
     2281 tgacaaaatc cttagcttgc gcaatctcca aggcctgcca gagtttctgg tcagatactt
     2341 cttgattgaa acctagagtc aagttggaac gaatggttcc tttaaagagt tcgacctttt
     2401 gaggtacata ggcaatccaa gaccgccact gctccaaatt aagaggacta cgtccatttt
     2461 gataaaggtc aatgttcccc ttgtctactg gataaagtcc aagtaagagt tgcaccaagc
     2521 ttgatttacc agaaccagtc cccccaatga taccaaggat ttgtccttga gtcatatcaa
     2581 aggaaatgtc tctcagagaa ggctgggccg catcaggata ggtaaaagtc aattcttgga
     2641 cttgtaaaac ccgaccactg gtaacttgct tttgttctaa ttctgaatgg atgttttcgg
     2701 gagattccgc aaagacctcc tcgattctct tggctgagat ataggactgg ttgagggaat
     2761 tgatcagcat ggcaagcttg accaattcca ccaaaatctg taagaggtaa ttgataagag
     2821 caatgagagc accttgactg agcactcctc cttgaattga aatatagcct tgccagataa
     2881 taacgagaag agttccattg acaatcagat aggtcagagg tgttaataaa ctagaccaga
     2941 aacctgtctt ttcttgtaat ctagcataaa cttggttaag ggtttgaaaa atctgtaact
     3001 ctcgtttttc ttgaccaaaa gcacgaataa cccgcatccc ttgcaattgc tggcgcgttt
     3061 cctgaaccag ttggtccgtt ttctttctga gactactgta gagaggattg accaatcgag
     3121 atagccctac aatgacaatg gtcaaaatgg caaccatgac taagaaccag aaagtcaact
     3181 cagctgagat tcgataagcc ataaaaatgg caccaaaaac gataatgggc gctcgcaaaa
     3241 agagacgcag gaattgattg ataccagtct gaatctggta ggtatccgaa gtcaagcgag
     3301 tgaccaaact agaagttgtc agacggtctc tgctgtcctt ggacaaggaa agaatatgac
     3361 gataaagatc gtttgtcaat tccttagcaa agcctactgc tgcctttgct gagtaaaatt
     3421 gagctatcaa ggccactaaa acgccaatta ctgcaaagat aaggagcagg ccaatctgca
     3481 tccagagatg accttgatct ccctgaggta aagattggtc aacaatccca gcaatcacca
     3541 tgggaaccaa gagctcaaaa acagcttcta acagcttgaa caagggggct aaaattgatt
     3601 ccttgatgta gggtttgaag taagataata ggtgtttcat aaatcccttc tattcatatt
     3661 ctagaaatga agaaagtggg aagccccact ctctgtttta tttgtttaag taaggcaata
     3721 gatagccata tcctgctttt tccatctcat ccttggccac aaagcgtaaa gaagcagaat
     3781 tgatacagta acggaggccg cctaactccc gcggtccatc tgtgaaaaca tgacccaagt
     3841 gagcactgcc tgaacgagaa cgaacttcaa ttcgctccat tccatggctc agatccttgt
     3901 aataatgaat caactctttg gaaatcggac ggctaaaact tggccaacca caacctgaag
     3961 caaacttatc cttggcaaaa aagagtggct cacctgtcgt aatatctaca taaatcccct
     4021 cttcaaaggt ttggtcatag gcattggtaa atggagcctc tgtagcagct tcttgtgtga
     4081 cacgataaga ctcttcagat agactggcct tcaacacctc ttgactaggc ttttcatagt
     4141 ttgctgcatc aatcaatggc ttatcagcat cggtcacatc gatatgacag taacctgaag
     4201 gattcttcct gagatagtct tggtggtagt cttcagccag aatgtagtgg cgtaattgct
     4261 ccacttctac tgcaatcttt cgacccagca tgcgttcctg ctcctgtacc actgtgtaga
     4321 tagctggcaa atctgcttca tcctgataat aaatcccagt tcgatattgg cgaccacggt
     4381 cattcccttg ttgattgata gatagaggat cgataactcg gaaataataa agtaaaatct
     4441 ctctgagtga cacttccttc tcatcgtaaa tcacttggac cgtttctgca tggtctgttt
     4501 ccttgatcaa ctggtaattg gtcgtttcga cttgaccatt agcgtagcca acactggttt
     4561 ctagcactcc agaaatgcgt gaaaaatatt cctctaggcc ccaaaaacaa ccacctgcta
     4621 gataaatttc tgccatcttt atttctcctt tatcacgttt ttaactaata ctcttcgaaa
     4681 atctcttcaa accacgtcag cgtcacctta ccgtatatat gttactgact tcgtcagttc
     4741 tatctgcaac ctcaaaacag tgtcttgagc tgacttcttc agttctatcc acaacctcaa
     4801 aacactgttt tgagcagcct gcggctagct tcctagtttg ctctttgatt ttcattgagt
     4861 ataaacttct tttcacaaaa aggatatgaa accctctggt caagagtttc cccatcctat
     4921 cttctattct ttattttgct tctacacgga caccttgggt atcaactctc aagtcatgca
     4981 gttttccttt gaaaggttgc ttttccaatt ctgccttaat tgttggcatc ttgtcatgag
     5041 aagccagaac cataactgtc ggcccagcac cagaaaggta ggttgcatag gccccatttt
     5101 ctttggtcac ttgcttaatc atcgcaaatt ctcttaccaa gtcctgacga tagcgctcat
     5161 ggaagaggtc tccctcgatt gcttgcccag cggtcaccat gtctcctgcc aacaaggcag
     5221 caaccgctac attggcgata gaacttgcag cagcagcttc cttataagac aattttttag
     5281 gcaagacact acggctgtcg cgagtacgta attcatagtt tggaatgtaa gctagaaaat
     5341 cacactctgg aaagtctgct acgatagcag agacttgccc ttcaacagaa cttgcaataa
     5401 cgagattacc ataaatggct ggagccacat tgtcaggatg cccttcaatc ttggtcgcta
     5461 actgcaattt ttcatggtct gataagttga gttgacccag ttggttggct agttcaatcc
     5521 cagcaacgat aaccgagctg gaagaaccca aaccgcgcgc caaagggaca tcactggtca
     5581 ttttcaagcg tcttggttgc aagtctggta caatttgcaa agcgattttg agcaagagat
     5641 tacgctcatc atgtggaatc catttgccaa tctggtgttc aatcagccac tcatctcgtt
     5701 cttcgcagac ctcaatttga agatacttgg ttacagctac accgaccgag tcaaaacctg
     5761 gcccgatatt ggcactggtt gcaggtacaa taatcttcat cttattctcc tagcaccttg
     5821 aaggtattca agaggtcgaa ttctgaaacc ttcttcaatt cagctgagac attttcaagc
     5881 tgggctttat taatcttgtg tgtgatgata acgacacgcg ccttgtcacc ctctttgcca
     5941 tcttgaagga tttgcttaaa ggaaatatct tgagcattga agatttcagc caacttcaag
     6001 acctgacctt ttgagtctgg agccaagatt gagaaatagt agtttgcttt gacatcttca
     6061 ggatttgcca agaccaagtc acggctatat tcgttgaagt ctttgccaat ggtaccatca
     6121 ttcaaacgac gaacgatacg gacaatatca gctacaacac ttgttgcagt tggtttttga
     6181 cccgcacctg gtccgtagta catagactca ccaataccga tagattctac aaagacagcg
     6241 ttcattacgc cattcacact agcaagtggg tgcgctttcg gtaggaaggt tggagtcact
     6301 tctgcagcaa tacctgaaga agtttcctca atagaaccaa ccaatttcac tacgtaacca
     6361 agctcttgag ctacagctac gtcttctggt gtgatattgc ggattccctt gtgggctaca
     6421 tcatcaaagg caatcttcat gccaaaggca aattggctca aaataaccat cttgtaggct
     6481 gcatcaatcc catctacgtc attcgtcgga tcgctttctg caaatcctag acgttgtgct
     6541 tccgcaagag catcatcgta agaccagcct tcttccacca tcttggtcac catgaagttg
     6601 gaagttccgt tgactactcc aagcacgcgc gtaattttat cagaagccaa ggaatttgct
     6661 aaagtacgaa gaattggaat cccaccagca actgctgctt cgtagtaaag tgctaccttg
     6721 ttagcttgag cgatttctag caattctgcg ccatggacag ctaaaaggtc cttgttagca
     6781 gtaacaacgt gttttccagc ttccaaggca cgagtgataa aggttttagc aggctcaata
     6841 cgccccatca attccactac gatagtaata tcctggtctg ataaaatatc atccacattg
     6901 gttacaaagt taaagtcatt ccctgctgca agcaagcgat ttttttcatc ttcatccttg
     6961 accaatacct tagcaacttt gatatctgaa tgtgctgatt gattgatttt tcctccattt
     7021 tcctttagga ggaaaggcac accacttgca acggtaccaa atcctagtaa agcaatttta
     7081 actgtcatct ttatctcctc gaaattttct aatatagcca ttataacaga attttgtgaa
     7141 aattcctatt atagtaaatc actatttcag tataaaaaga aaaaacgaat cagacgattc
     7201 gctcttctta aaatctgaaa atagctttcc agaaaggatt agccgatttt ttgcagattg
     7261 agcactgcat cgtgactcat caagacttga ccatactctt gtaagactga gcgactgata
     7321 tcactatcgt ctgcaaactc gcgcatacgg gccaacagcc aagctggata tgggcttgga
     7381 tgattttcaa tatccactaa aatggtcaaa taatagcgct cgttcatttt gtagagttca
     7441 gaagtttcca tttcaaaagt cactgtcttg gcaaaagcta ccaagtcagc caacttagca
     7501 aaagaaagga tgtagtagat gtaaggttct ttcttactct cagcttcttg ttcagcctgc
     7561 tcttgctctt cttccttggc ttcaacttgc tcaagagatt gaatggcttc gatatcatcc
     7621 ttggttttgt ctgcgatgct tttttccagg gttttgataa attcatctgg agacatttga
     7681 gccaattctt ccatatctgg caaatccgat aagtcttcaa aatctagatt ttggtcaatc
     7741 tttgacttgg tcacaaagac atctacctta tcaggttttg gagtcacacg gaagctcaac
     7801 atgcctgtat ccagaaagct atcaggcatc tctagctcat ccaagatagc ataaaagaac
     7861 tcttctgttt tttcttgagg aacgagaaag tcagcaatct ccattccacg atccatcaaa
     7921 tcctctaaag acatcgtgat ttttaaagtt gtatcactaa tttgtttcat tttcattgct
     7981 agtaacctca tactttcagt tctatctatt atactagatt tttacgattt tatcaaaaga
     8041 aggctcctct atacggatag attttcccta gggtctttct ataggagact ccaagagaaa
     8101 atttctgcag acagatagaa aaagccttca aaatcggctc ttagccgatt ttgaagacct
     8161 tatacatcag aatacttata atttaaaggt tgctacaccg aggatagaac gatttaagtt
     8221 tctgagaatt tgaagacttt gctcaaattt cttataacga gtcactccgt actcttcaac
     8281 aagaaggact gtatctcttt ccaaaagaga tgatacatcc tgcaaatcta caaaatgcat
     8341 tccttttaaa gcttcttgac tctgtttcaa tttatctagg atagctttat ttgagctaac
     8401 gatggtcaat tcctgtccag tatttttgta tgacaaaaca tctgctaggt tagcaattgt
     8461 tgtaatctct gttacaaaat caatttgata ctgagaaaaa tcacctactc tattgattgt
     8521 tggattaaag agataaacta acacatttcc catcacaacc aaaatcacac aaaccactcc
     8581 aataacaact aaacgaagaa tcagattttt cacatttaag ccaagcgctg tttcaccatt
     8641 tgcgttcaat tctttagagt tgatggtttc cagtttttca attttcacat ttgcataagc
     8701 atgtttaaat ttctcaatca acccatcaat ttttttctct aacaagtcat tggcatcttt
     8761 acttgatgtc aaaattttca caccaacccc tgcatcgtca atcatatagt agacggtcaa
     8821 ttttttccac caatagtcat tcgttgaatt tttcaaggtt gtttccgtcg tgtctaattc
     8881 actggcaatt tttttcaact cactgggttc tacatcattg aaaagataag ctccattcaa
     8941 attaccatca atcaatttcc cataaaaatc actataacca ccaatttgat gattcaaaat
     9001 cgttttggcc gaatcttctg gaggagtgat tttatagata agataagttg aataacttgt
     9061 tgtatctttg acagtgtttt tattcctaac tgctttaatt gtaaatggta cagcaatgag
     9121 agcaaataaa gcgatgagag ctaaaatatt tgcttttcgt tttttataaa gatttgcaaa
     9181 caaatcagct actgaataat gttcaaacat gatttttttc tcctttgttt agtagatact
     9241 agttttcctt tgtaagcatt tttgctacaa atataatcac aagaacaatt ccccagaatt
     9301 gcattgtaaa taaattgaag aaactttctg aaaagctgct tcttggcata aagaatagat
     9361 tattcaagat gagtagggat aaagcaaata ggattgtcct tgagcgatag gctacttgca
     9421 gcatggctat aaataatacg ccgagtaaga aactaagcag aaagactcca atcataccat
     9481 agtcggtata caactccatg atataactac ttccgatacc atgccctttc aagtattcct
     9541 tgttcaagac aagataggat agattgtggg cataactatt actatcaata gctagttcca
     9601 cactattggt tgtatgttca aaggcttttc ctccgaaaat ggctcccaaa ctcccccttg
     9661 caaaataatc aagaacagga ccaaaagtaa aattacggaa atctcggtaa gggaggctac
     9721 tgttaaatag aaaacctcga gccagaacac caaaactagt cccttgttta tagataaagt
     9781 caagtaagat atcccagaaa cctgtatgag aaacttggac attatcccgt acataattga
     9841 gtactcccat cgctaacatg agaataggag aacctacaaa aatcgctaac ttttctttaa
     9901 acccaatcca ttttcctttt tcagtttgct cccgcataaa gtaataaaca aaagcaaata
     9961 aaatacttaa aataaaggga tttcgtgtcc caattgccaa atgaatagta ttagctgcaa
    10021 taaaggagac aagcactgct gtggcctgca atttctttgg cttggttgcc agatacatac
    10081 acattgcata gaccgtaaag gtagacaaaa tgtaggtaaa ataaggcagt ttactttcaa
    10141 aatttgcata gtaggcatag taggaagtct gcaaacgata caagagccgt tcaaataacc
    10201 gaatgaaata gaaaggatag gttagaagaa aaactcctag tgatacaaag cgtaaccgct
    10261 tgatataaac ctcttttaga gaatttccta tatttgctac ttttattttc ttcctagcta
    10321 tgaagtaacg agccagaatg cctcctgtgg tcaagcccag aatcgaaacc atgacaacta
    10381 taaaggcaaa acgataggct attggatgat aggtatccaa agcaccatcc ctaaaataat
    10441 caatggtcgg tcttgatacc agaaatacaa aaatggttaa atagaaaata aaatggatta
    10501 agtaatactt gatatcattc caacaagcaa ttaagctact aaccaacaag aacaataaag
    10561 tagaaagtaa gctaacatta ttattattat taaacagata cacaattcca cttactagcg
    10621 tcaaggcata actgactatg gtcaaactaa ataataatcg tttcccatca atcacttggt
    10681 cacccccgtt ctaatgtaat tttttagatt tttcaatatt tttcagtaat aagaatcgat
    10741 ataaggaaat atttatgaat agggccaaag cactaattct tctcccctta cggaaaattg
    10801 gattcctaga aatagcaaag gcatggcctt ttaaaaaacg atgaatctga gaataggctt
    10861 caaactgttt atactgatca tctagcaaca tcttatccag aataaagaag tgggcatagg
    10921 ccaatctgaa aaaagcgacc tctttcaagt caggatagtt tttcacaact tcattataaa
    10981 acttttggta gatatcaata taggctaaat ccttctctgc atagggtttg gtcgtaatac
    11041 tatcccctct atggaaatag taataatagg gtttagtatt aaccacatac ttcttggcca
    11101 acttgattaa atcaaaatgg taataggcat cttcgtaaat caacccctta ggaaaggata
    11161 gggcagttgc aatctgtctc ttgattagct tattgcaaat cgtcccaggt attttttcac
    11221 ctatgaggta ttcctttaga aatgtttgag aatcacagac aaaatagtca tcctgattgg
    11281 ctgactgtgg gctttcatca ttagcataga cattcatgac accacagctt gaaacatccg
    11341 catcttcttg aactaattgc tcatataagc tctgaatcat ttctggatgg atataatcat
    11401 ctgagtcaat aaaaatcaga taatccccgt gagcctgctt catcccatca tttcgtgctt
    11461 gcgacaatcc ttcgttcttt ttatgaagca ctgacaccct gtcatcttgt tcagcgattg
    11521 aatcacacaa gcgaccactt tcatctgttg caccatcatc aacaagaata atttccagat
    11581 tttgataggt ctgcttctga atggaagcta tcgatttttc taggtactgc gccacattat
    11641 agactggcac aatcacacta attaatgcag tttccatgct actcctctaa tagtttttct
    11701 acttgttcga tttgttttgt aattgtaaat tgttgaatga attggctagc ctcatcgaca
    11761 tcaaagtttg aggcagaagt catgtaatta gtaatcgcct gagctgcctc ttgattgctc
    11821 tcaatgattt gtccaaatcg tccttcttgg gataattcct cagcccctcc aacgtccgta
    11881 gagataaaag ggagtcccag actcaaggcc tccacataca ctccaggaaa accttcttgt
    11941 ttagacatag acaagagaac tttcgtctga gatagatact gataaggatt tttttgataa
    12001 ccaaggaaat gtacatagtc ctcaatccca tactctttga ctcgtttttt cagttcctct
    12061 tccatatcac cagccccgat aaaatagaga tgatagtttt ttccctcttg gtgtaataat
    12121 cgtatcactt ccactacacg gtcagaaccc ttattttcct caatccgtcc gatagtacag
    12181 atactttgag gagcaatctc gatatcgatc ttctcttgag atttttctag aatagtctga
    12241 aaatcatatc cattgtagat tgtctgtaat ttagaagcat aatctggata aacttccttg
    12301 atagaattgc tggtcttttt tgaaatccct acaattgtat tcgcagcatc caactggctt
    12361 ctatgtgatt ctcttttaga gctatcctta agaagttctt caatacttcc atgaatccaa
    12421 gatatcttct tgacttctct tcttttagag aacaacagtg gtggattcat aatggtaaaa
    12481 gaaacttcaa catcataatc atcttttaca agcaaacgac gagccagtct tggaaaataa
    12541 attctcattc tccacaaaaa agctcgtaac catctggttt ggcgataatc ttgaagggat
    12601 tttaaaatgc gtacatgctt tggaacagat tcatatccct tgtcaaagtg ctccatttca
    12661 agaatatcaa tatcatactt ttctagatcc agatttgaaa caatggttga tagaatcttc
    12721 tctgcaccac ctccaagaga aaaagaccac ataaaaaata agattttttt cttagccacc
    12781 atattctccc ttgtattctg tataagactt atccatatca gcgatgacag catcatgatg
    12841 cggtacctgc ttgtctgctg gtggaggcgt catataatcc ccaaaagcag ttctgagata
    12901 gacatcatag ccgattggaa taggcatctc tgttccttca aatggcaaga aaagattgtc
    12961 ttcaaaagat gtgattgggt acttgtttct catgtagcca ggacctgagc ataattctgt
    13021 aatgccatca caatcagcca aatcatactt agtcatttct ttctcagctt ttttccagat
    13081 gcgataacgg agagattttg gagtcaaacc cagtaaaatg cgacttcccc atttcatgag
    13141 agcaccatgc ttttctggaa tagtttgcgc acaaaagagt gaataaatca aggcccaacg
    13201 aacctgtttt ttccgctcag ctggatcttt cggataataa tccaaaggca aaacatccaa
    13261 ggccagacca tgtggcaaat ccaaatcctg ctgataaggc ttgatacagg tggtttcctt
    13321 gtcacgaatg gtaataaaaa gattacgatc aacaaaatcc ttgtaactct ttgacaagaa
    13381 ataacgttca tctgcataac gaggccataa ttctgctaat ttctcataat ctttacgagg
    13441 cataaaaaag tctaggtcgt cgtcccaagg aataaatccc ttgtttcgaa gggtaccaat
    13501 agcgcctccg ccacagagat aacagagcaa atcatgttct ttacaaaagg ccacaaaata
    13561 ttcagccatc tccagactac gagcctgaat tgcttttaaa tcagtcatat tgttcattat
    13621 tctttctatc gtatcgtttc attataccac aaacaagggg tgaaaatcta ttgcagactg
    13681 taaaaaatca aagcctgact gctatccaaa tagctatcaa actttgattt ttctgtctta
    13741 tactcttcga aaatctcttc aaaccacgtc agcttcacct tgccgtaggt ataggtaact
    13801 gacttcgtca gtcttatcta caacctcaaa actgtgtttt tagcagcctg cggctagctt
    13861 cctagtttgc actttaattt tcattgagta ttatcttatc tcaagcccat ttgagcgagc
    13921 ttggtttgat atttgttttg atcaaccagc aagcccaagc ctccataaac atcataggca
    13981 tctacccagt cacccagttc tggaatcgtc aatttttcaa taccattttt tgctccatcc
    14041 aaaacagata aaccgtttgt taggaggaaa gtatagggta cgttggttga ggtcatagca
    14101 aaaacctttc caagagcttc agaaccagtg aaaagtttag tgggatcttt aatttgctct
    14161 aaaattgctg ttaaaacttg ttgctgtctt tttgtacggc cgtaatccgc ctcatcatca
    14221 tcacggaaac gagcataatt gagcagggtc gagccattca tctgctgttt tccgacttta
    14281 atggtttggg ttggagactc agtctcggta gcatataaat catctccgac tgtagcttct
    14341 gttaggggac gcccattcaa tgttgaaaat tgagcatcaa tcgtcacccc atcagggaaa
    14401 agcgtgtcaa tcgctgtggc aaaggcctgg aaatcaacca aggcgtagta cttaatgtcc
    14461 aagtcaaaat tatctttcaa gacttggcga accatttctg cccctttttg cccctcttgt
    14521 tctcctaact cgtaggctac gtttaacttg ttatctgtct gttttctacc attaatcact
    14581 tgactataac catctatata gaccaaatta tcacgcatga aactgactag cttcattttc
    14641 ttatctgagc ccccgacatt taataccata atagagtcag ttcgtgtctc aacactgttc
    14701 tggccgattc gaccatcagt acccatgatt aaaatattaa ctccatctct agtgtcctga
    14761 ccattaaata cttctacttg agctgcccgg gcatcagcag ttttctttgc gctagcatct
    14821 tggtaaccac gcaaaaacat gaataccatg gccaaagcca cacagaccaa aagtgaaaaa
    14881 atcaccataa aaattcgttt aagacggagc ttccgtcttt tcttttttgg agggaaagag
    14941 agtgcttgtg atttggattg tgagcgactc cggttcgcat agcttggtaa gtcaacctgc
    15001 tcttctcttt cttgttccaa gctagagcta ctatttcccc tagcaagagt tagcttttct
    15061 tgcaaatagg caaactcatt tttttctctc tcattgagat agtgaatatt ttttagcaaa
    15121 taatcataac gcaactgctc atgatgactt aagggatttt ctttactcat cttctctcct
    15181 ttccatggtc tgatattgga taaataggat aggcacccag aattttatac tggattccaa
    15241 tcgcttctaa ttctttttgg gcaaagtgga ccaagtcctt atcggtataa tccacatcga
    15301 taatgaaaaa gtattcaccc agtgctgtct tgagtggacg actttcaatt tttgtcaagt
    15361 caattcctcg ccaagcaaag gtcgacaggg ctttataaag tgcacctgga aggttgtcag
    15421 gtagggtcaa ggctaaactc attttttcag tttgtgattg caagggaata gttacacctt
    15481 cagctcccaa aacccaaaaa cgtgtgaaat tggcttccat ttcctgaata tcctcggcaa
    15541 tcagttccaa tccatattct tcagcagaac ttctaggtgc aactgctgca aagggctgat
    15601 ctggatgttc ggaaataaaa cgggccgcat aagctgtact agctgttacc tcgatttgag
    15661 cctctggata ttgttcatcg atgaatttct ttccttgagc caaggcctgt ggatgtgaaa
    15721 aaatcttttc aatcttagta tggcctggaa ccaccatcaa ctgctgatga ataggctgaa
    15781 cgatttctgc tactgcttgg atgtgagcct gatgaaaaag atagtccaag gtttcatgaa
    15841 cactaccctc aatagaattt tcaactggca ccacagaata gttcactaat ccttgctcat
    15901 aagccttgat gacatctgta atgttggcaa aagcctgcaa ttcctcatga ggaaaagctg
    15961 tctgcacaac gtggtgtgaa aatgatccct tgggacctag ataagctatt ttcatcttag
    16021 ttcctctata atttcctctg ggcttagctt ggtcacatcc aaaacccgac tagccacttc
    16081 ctcataccaa gcctgtcttt cttgaaaaat agctactagt tcttccttgc tattatttag
    16141 aaaaagcggt cgctgattgt ccttatcagc tgcgatacgt aggtagaggg tttcaaaatc
    16201 tgctttcagg tagatgttat ctgtattagt cttgagtaag tcacgatttc tctgagaaat
    16261 aaccactcct cctccagttg acacgacttg gtctgtttgt agtaaatcag ctaggacttc
    16321 tgattctacc tgacgaaagg ctgcttctcc cttttcagcg aaaaaattcg caatggacat
    16381 acctaggcgc ttctcaatca gagcatccat atcaaggtaa ttagggtcca agcctcttgc
    16441 aatagtcgat tttccagccc ccataaaccc taataacacc ttagccatga atcaagctct
    16501 ccaaatcatc aaagaaacta ggatagctgg tattgatggc ttctgcacgg tcaagctcca
    16561 cctctccatc tgcaaccaag agggctgcga tagctgtcat catgccgata cggtggtcac
    16621 caaacgtatt gactctagca ccgtgaagag ctgatttccc tttgataatc atcccatctg
    16681 ccgtaggagt aatatctgct cccatactat ttaaggcgtc tgccacaacc tgaatacggt
    16741 ctgtttcctt gaccttgagc tcctcagcat ccttgataac tgttacacct tgggcttgag
    16801 tcgcaagcag ggcaataatg ggcaattcat caatcaatcg tggaatcaaa gcgccaccaa
    16861 tctctgttcc tttcaagtca gaagactcaa caatcaaggt agcagattta gcgactggat
    16921 cgatttcagt tatttccaat tttccaccca tggcacgaat gacatcaata ataccggtgc
    16981 gagtttcgtt gatccccaca ttctgcagca ctaaacgaga atttgggaca atcaaacctg
    17041 cgactaacca aaaggatgca ctggaaatat ctcctggtac gaccaccttt tgtccagtca
    17101 gtttttgagg tccttgaact gtgattttct tgccatccac acttaaatgg ccaccaaatt
    17161 gttgcaacat atcttcagta tgattacggg tgcactcttt ttcgataata actgactccc
    17221 ccttagcttg taaggctgca aacatcaagg ctgacttgac ttgggcagag gcaattggca
    17281 actcataatg aataggtctt aggtttttcg tcccttttaa gcgaagggga ggcaagtctc
    17341 gttcagtttg ccctgagatg ctgacgccca tttttttcag tggaagggtc acacggtcca
    17401 taggacgttt ggaaagacta tcatctccaa acatctctac ttcgaaatct gcaccagcaa
    17461 ggacacctga aatcaggcga atcgaggtgc cagaatttcc catattaagg gcattttgcg
    17521 gcgcttttaa gccagccatg cctacacctt gaatggtaat aaccccatct ttatcctcaa
    17581 tttcaacacc aaggtcacga aaaacctgca tggtcgaaag aacgtcttca cctcgcagaa
    17641 tatcataaac cttggtctca ccctcagcca aacttccaaa gataatggaa cggtggctga
    17701 tagacttgtc acctgggacg cggatactac catgtaaatg gcgaatattt gtttttagtt
    17761 tcatactgga cctcatactt gcaatacttt tacctatttt atcataaaaa gccagaaatt
    17821 ccttaaaaat tcctgacttt aggatagttc ttttcttatt tcagcaattc tgaaactggt
    17881 tcgaaaacaa ttttttcaat gtcagaaagg taaatggcca attgttgttg cttggtaaag
    17941 aattctgaca agaggctatt tccttgaatc tgtttaccaa agccttccat cttagcttgg
    18001 aaggacgcat ctggcatttg acctgtctgt gctagttttt gaatttcctc ttgaaaggca
    18061 agatattctg taaagatttt gcttgcctca gcatctgctg caatcgcatc tttagctgct
    18121 ttaacagcct tgtattctgg taatccgcgt agaccgcgac tgagttcgtt tgcactatcg
    18181 taaatatttg acatgttctt ctccttattt aatgacgact gtatagtcgg tattttttgt
    18241 tatgagatgc tcggctcttt ccaagtcttg agcattttta aatgaaattt gtaggattcc
    18301 gtgaatatcc tcacgatttt cctcgttgat gtggatgtta accaaggaag tcccacgtag
    18361 cagttccaaa atccgcagga tgacatcttc ttcatcagga acgtcaacat agaggtcgta
    18421 agagctatcc acaccaccac gcttatggat ttccatggtc tggcgttgtt cacgcgcttg
    18481 gttaaaaaag ttccaaattt gctcttcatc tcccttacta atggcctgac caatcgattc
    18541 caaacgttcc ttgaaatcct caattctatc cagaatggtc tcgctattgg acaagagaat
    18601 ggaggtccac attcctggct cgctttccgc aattcgggtc atatctcgaa aaccacctgc
    18661 cgcaaagcgc cttgccatct catgctcttg agcatagacc gcagtctgct ccatgagact
    18721 agaagccaaa atatgaggaa aatggctaat ctgagaagtg acacgatcat gctccttggc
    18781 atcaatctcg ataaaacgag catgaagacc tgaaagcaga tccttcattt ccttaagcgt
    18841 gtcctgactt gtcaggcttg aaggtgtaaa gatataatag gcattttcaa aaagattgac
    18901 atctgccgaa gcagcccctg tcttgtgact accagccatg ggatgggccc cgacaaagcg
    18961 aacagacttg ccagccaaat actgctccgc cgcatccaca atggttgact tggtcgaacc
    19021 agcatctgaa ataataacgc cttctcgcaa atccaaattg gccaactcct taatgaaagc
    19081 aatagtttgt ttgattggca agctgaggat aatgacatct gccaaaggag caaaactagc
    19141 aaaatcatcc gttgcacggt caatcatgcc ttctttcaag gcgatatctc tcgaagcttg
    19201 actacgatta taacctaaaa tttcataatc tggatgatcg cgtttgatac caagtgccat
    19261 agaggctcca atcaacccaa gacctgcgat atagattgtt tttgccatag gaactcctta
    19321 atagttcttt gtatagtctc ggtgtttggc taccgcttct tttagttcct caagattatc
    19381 tgatgagaat ttttcgagga tctcttgcgc cagaaccgtt gctacaactg cttccatgac
    19441 cattcctgca gctggaagag cagtcggatc acttctctcc acggttgcct tgtaaggttc
    19501 gtgggtttcg atatccacac tcataagagg tttataaaga gtaggaatgg gtttcatgac
    19561 cccacgaaca acgatgggtt gcccattagt cataccacct tcaaaaccac ctagattatt
    19621 ggtacggcga gtataaccgt cttctttaga ccagagaatt tcatccataa cttggctgcc
    19681 tttacgataa ccagcctcaa agccaagacc aaattccacc cctttaaagg cattgataga
    19741 gacaacagct tgagccaatc ttgcatccaa ttttctatcc cattggacat aggaaccaag
    19801 accaactgga actcctccga cgactgtctc cacaacccca ccgatggtat caccatcacg
    19861 tttgatttgg tcaatatagt ccttgatttc ctgttctcgt tcttggttga caatagaaac
    19921 ttcagactgg gcagctcttt gcttaatttc agcgactgtc agattttcag gaacatctat
    19981 ttccttacca ccaaagacca cgacatggtt ggcaatctcc atatccagct cagccaagag
    20041 gcgtttggct actgcaccaa ctgccacccg catggtggtt tcacgagctg atgaacgctc
    20101 caaagaattt cgcaaatcat caaaacggta cttaatcccc ccaaccaaat cggcatgacc
    20161 tgggcgagga tgagtaattt tccgcttgct tttaaggcgg tcttcaatgt cctccgcaga
    20221 catgatgtcc agccatttct ggtggtcctt attgatgaca tccatagtaa taggcgcccc
    20281 tgtcgtcttc ccgtggcgaa cacccgaagt aaagacaacc tggtcactct caatcttcat
    20341 acgaccacca cgaccgtagc caccctgacg gcgtctaaga tcctcattga tatcctcagc
    20401 tgtcaatgga agtccagctg gaattccctc aataatagct gttagacggg ggccgtgtga
    20461 ttctcctgca gttaaatatc tcatacactc tccttatttt accaagtagt ctttcatctc
    20521 ttccagagaa actgggtgaa tggtcgctga accaagctct ggcaccaaga ccaatttcaa
    20581 ggtgttacca cgcgctttct tgtcatgagt aagagcctga taaagcttgt caactttcca
    20641 attttcatag tcaacaggca agccgaattt ctgacacatc tctgtgatag attgggtaat
    20701 gccagctggc atgaggcctt tttcctcagc aaccttggaa atctgtacca ttcccatggc
    20761 aacagcctct ccatgcatga ccttgccata accggcagtc gcttcgatgg catggccaat
    20821 agtgtggcca aaattgaggt aaagacgaat accattgtcc aactcatctt caaccaccat
    20881 cttgcgcttc acctgacaag aatgttcaat caaggtctct gcatgttcca aaatactctc
    20941 aacagaacca ttcagtcccg tcaagagagc ccacagttct ggatcctcaa tcaagccata
    21001 cttgataact tcacccatcc cttcaatcaa ctctcttttt ccgagggttt caagaacaag
    21061 tggatcaatc agaaccccat ctggttgggc aaaggtcccc accatatttt tagcaaatgg
    21121 tgtgttaacg cctgtctttc caccgataga agaatcaacc tgagctgtca aactagtcgg
    21181 aatctgaaca aagtgaatac cccgcatata ggtagaggct acaaatccag ccaggtcccc
    21241 aacaacgcca ccaccaagag caacgattcc atcgctacga gtcagacctt gcttgactag
    21301 aaattcatag actttctgaa cagtagttaa attctttctt tcttcacctt ctaagaaatc
    21361 aaaaacagct acctgaaaac cagcatcttc taggctgagc ttgaccttct ctgcatagag
    21421 agaggctaca tggttatctg tcacaatgac taccttttgc ggttgccaga gttctcgcaa
    21481 ccactgacca gcctgggcca tacaaccttt ttcaatctga atatcataag gatgatgagg
    21541 aatatcgatt ctgattttca taggagagtc tccctttctt tattggtatt tttctgttaa
    21601 agactgccaa atctcttctg tcggcatttc cttgcctgtc cacagttgaa aagcttctgc
    21661 agcttgatag agcaacattc ccagaccatt gactgctgga ttgccctgac ttctagccca
    21721 tttcaaaaac ggtgtttcaa agggttggta tatgatatct gcaactaaga gagtttctgg
    21781 taagactatg ttttcaggaa caggagagga ttggccatcc atgcccacac tggtggcatt
    21841 aactagcaaa tccgactcgg caatccttgc ttgcagttca gaaacatatt ctaaagcaca
    21901 caaatccact ttaaaacctg tctgctcctg taacttgtct aggtaaggtc ttgttttttc
    21961 catagaaacg gaacgaacaa agaccgaaat ctgactgacg ccatccaaaa tagcctgtgc
    22021 caagattgat ttagccgcac cacctgcacc cagcagggtc atctttttac ctgaaattgt
    22081 aaaagaaggc aagcacttaa aaaatccctt gccatctgta ttatatccaa ttaaattgcc
    22141 attctcattg acaaccgtat taaccgcacc aatcaagtgc gcttcatcgc tcagcttatc
    22201 caaataagga atcacctgct ccttataggg catggacaga ttgatgccaa acatctggta
    22261 gcgacgaata ttggccactg tttctgccaa gtcactcgct tcaatctccc aagccacata
    22321 agcaccgttg gtagctgtcg cctcaaaggc tctattgtgg atgaagggag aaatagaatg
    22381 cttaatagga ttggcaacaa ctgcagctaa acgtgtatag ccatcaagct tcatccaaaa
    22441 tctccctgat ttttttcatg ctagctagag aaatctgccc aggggcacta acctcatcca
    22501 gactggcaaa agaccaactc gaaccagtca catccgcagt gatacgagag accttgccca
    22561 ccttacccat agaaatggtc acatattcct gttcaggatt gagggtttta aagcctcgtg
    22621 tatagttcat caagtctaag acatcctgct ccgtgtgagc catcaccgca accttaacaa
    22681 gttttggatt taggatcgtc aactctgaca agatttccat catgttctca ggtgtttctt
    22741 ggaaattatg gtaactcaaa acaagatttg ggaagtccag catttcctca aaaacatcct
    22801 tgtagctata gtactcaaaa tcaatatagt ctggttgata gagttgcgca acttccttga
    22861 ttagatggat atactcttct ggagaaaggt cgatttctcc accttcggag cgagttcgta
    22921 gcgtgaaaac caactcacgg cctgcgaatt tttcaaaaat ggctggagct acctgcaaaa
    22981 tcgcttcttt aggcagatag tcggcacgcc attcaatgat gtcggcatcc aggtacctcg
    23041 tggcatccag agcctgagcc tcctctaaac ttcttggcat tactgaaacg attaatttca
    23101 tttactaacc ttcatactaa tcaccttgag gtaattacta ctttcatctt ttttattata
    23161 ggcaaaatct gctggaagac catatttgtt taaaatctgg taacttcttc ctgcaaaacc
    23221 tttatcaatt tgttctgtaa atttctgacg ggaaacattg gcagcattgg tactggcaat
    23281 gataatccct cccggattta aaatctcaag actctgggaa atcaacttgt gataatcctt
    23341 ggccacagag aaagtttgtt ttttattccg agcaaagcta ggcggatcta ggacaatcac
    23401 atcgtaggtc aagtctttgc gtttggcata tttgaaatac tcaaagacat ccatgactat
    23461 aaaacgatgc tcgtctgtgc tgagcccatt tgcctgaaaa tgcgcttgag acaattctcg
    23521 tgaacgtttg gctagatcaa cagaagttgt ctggctagct cctcccatgg ccgcagctac
    23581 tgaaaaagcc gctgtgtagg aaaacatatt gagcaaggat ttacccatag ccaagccgtc
    23641 aactaaacta ccgcgaacct catgctggtc taggaaaatt cctgtcatca agccatcatt
    23701 cataaagact tgatacagga caccattttc taaaacagtg aaaaagtcag gtgcttcttg
    23761 accataaaca tgggcagatt catagtccaa acccttaaag cggattttct cataagctcc
    23821 taaaacctca gggaaaacct gtctaaaggc ttctgatata gtctgacgaa tctgataaac
    23881 ataagagtta taccaagaaa agacggcgta gtcgccataa aggtccactg tcagaccccc
    23941 aaagccatct ccctcttgat taaagagacg aaaggcagtt gtcaaatcat cttgatagta
    24001 ggcgtttctc ttttctttgg cttttctaaa caacgtttca aagaaagctt gattgaaggc
    24061 caccttgtct ttgctgataa accagcccaa gcccttgttt tgctgagaaa ggtaggcagt
    24121 cccaagaaag tttccttcct gaccctgcac ctctacttcc tgatccttaa gattgacatt
    24181 ctcaagatca ctggcttcta gtaaaactag ccccttagca agcttctttt caaccctttt
    24241 gctgactctt attctattca taactaccat tatatcaaac ttttagacaa ttctcaaaaa
    24301 agaaactacc cttgcttttt tactcttctt ttaaaaaatg gtatactaga cttcctgcaa
    24361 aactaggaag taaatgtgta agaatcacag taaaaaatgc tcttccgtct tggaggagca
    24421 tttcttttta tcaacgaaaa tcaaatagca aactatgaaa ctagcctcag gttaaccgtg
    24481 agattatagg tagagaggtt gtatcagcaa tatgtgtctg tcaaatttag tgacaaaggt
    24541 agtagaagaa agatgaagaa ataaatcagc ttcagtagat atctggaaaa tttgatttta
    24601 tagaaaagcc ttttgttaca aactcaatat actatcaata aataatatta tagaagcaac
    24661 aataattaaa atttcaccta tttgcatcat tctatttcga actctaaata tatgttctat
    24721 caaaaatact tggatcacac acattatagg aattaacgtt tttgaaattg aaaaatatcc
    24781 caataaataa actataaaca acaataatag aactatatta tatttcttat tcaaaacatt
    24841 cctccctata tttttgatta ccaatcttaa tcatttacaa ctacattcta acaaactata
    24901 aaagcgtttg tcgaattgaa tttatcaagc aagcaaccaa ccagctcatc ttttttctat
    24961 ttctgccaat atgcgtgaca ggtaataatg atagccaaaa atagcaagag caagcaagac
    25021 gataagagct cctactccca agttgatggc aaggataggg gagagagact gaaccaagaa
    25081 tatgctccca attacaaggg ccatcaggat tgcactataa ataaacaata aaactatggc
    25141 gactatgcca tttgaacgat tcaccaggtc cgtaatgcta ctccaattgg ttgacagatt
    25201 tttaacgtcc ttaaagtaat ggtggcaaga aaggatgaca ctggcaagga tccatgccac
    25261 aataaggtaa atcatcgaaa tgatgggcaa gcctagatat agagaaagac caagcaaagt
    25321 cagaactggt aaaaaggact ggacagcata tataatccaa aatttcactt tcacataacg
    25381 agcaaagtca aagggtaaac tcttaagaaa atcaacattt tccctctcca aggacaaggc
    25441 aattgaatgc aggctggtga tattgttatt gacaactgct ataaacactc ctacaaaaaa
    25501 caagggtaac cagtatggag gatgaatgtc tggaactatc tgagaatctc ggattttgga
    25561 aatcagaccg atcatcatga gataaggaaa gaaagcactt gtaaaaagca ctgtaaccac
    25621 gccagtaccc tgtcccaaga gggtgaggtg gtagcgtaaa accatgcgga aaaatccctt
    25681 tttagtggtt gaaattctct ccttgctgcg acgttctttt ttgaccttct cctcactatt
    25741 aagcaggatc acgtcataaa aacgaggaag gaccttcttt ttggtcagat aaagcaggaa
    25801 gagagttagt cctatccaag cgagcagacc caatatggct tctgtcgaaa aaggatccac
    25861 tgctattttg taaaagatat gaagaggata aaggagaaat ggaatgtctc taactttgtc
    25921 aacaatactt ccaaaagtcg actgaagaaa gaagataaat attaaaggta tgagaactcc
    25981 tatcccaatc atcacattcg aaaaaataga ctgatacttt ctgaagaccc tagtttgagc
    26041 caagaaatgc actgccacta ccatcactag agccacagag acaaataata aggtcaagga
    26101 cagtagcatc aaaggcaaac ccagccatag agaaggagct agcctaatgt agaggaccag
    26161 aaaataagct aggattggta caattccagt tagagctggc aaaaggacag acagtccttt
    26221 agcaattata atctctgatt ctttaaaggc atagggccta tacgatatca aatccttact
    26281 ctcataaaag acattgtaaa aggccgttaa agaagttgaa aaggcaatca ctagtaaaat
    26341 agcaatcatc aagctaaaat aaataggtat ttcctcaaaa ggaaaatgaa tggctatatt
    26401 actaaaacag atgatcatca agagactgga aaaaatgtaa gaacttaaga ctctagcgga
    26461 aacatttact tttttcccag gattcttagc ctgcttcttt cgtagattag ccagattagc
    26521 ttcttgagat gaataaagga tattgatatc aactaatttt ttaatgacct tgagacgcat
    26581 ccgcaacctc ctcttttcta ccagcaagac taaggtagat actttccaaa gactggtctg
    26641 ggtaatcttt tctcaagtcc tctacactac cacaataaat caaatgcccc tttttcaaaa
    26701 tggcaatccg atcacagact tgctctgcca cctctaggac atgagttgaa aacaagactg
    26761 tcttcccttt ttgtgcatgt tccttcatca tctgtttcaa atcaaaggca gcctggggat
    26821 ccaaaccagt caagggttca tccaagaccc aaatatcggg atcagacaag agtgctccga
    26881 tgacaaagac tttctgacgc attccgtgag aaagagtttc aataacctga tagcgatttt
    26941 cagcaaaatc aaaaacgttc aatagcctag ctagactagc ctccaagtca gatctactca
    27001 gatcatagga tgaggcgatc aattcccaaa attcattggc cgttaagcgt aaaaataagt
    27061 caggcgagtc tgctacgtag ccaatctttc gtttaatagc caagcgattt tccgataact
    27121 cctgaccgtc taccaaaata cgaccactgc tgggtgaaat gatactgact agggatttta
    27181 tagtggtcga ttttccagcc ccattatgac caatcaagcc cataatctct ccattttcaa
    27241 tctgcaaatt gagattactc aaggcctttt tatcaccata caacttacta acattttgaa
    27301 attcaatcat gggacaactc ctatctttat ctgtattata tatcgctata ctagcacttt
    27361 gatacaaaaa aatcaatact ttattttaag tagttaaaat agtttccttc tatcttacgc
    27421 aaacgtttgc gctaatagat actttatgat agaataaaga acaagattga caagtaagag
    27481 gaaacattat gcaaaatcaa acactcatgc aatactttga atggtatctg ccccacgacg
    27541 gtcaacactg gacgcgtctg gctgaaaatg ctccacacct agctcatctg gggatcagtc
    27601 acgtctggat gccaccagcc ttcaaggcaa ccaacgaaaa agatgtcggc tatggggtct
    27661 atgacttatt tgacttagga gagttcaacc aaaaagggac tgtccgcacc aagtatggtt
    27721 tcaaagaaga ctatcttcaa gccattcaag cccttaaagc gcagggaatt caacctatgg
    27781 ccgatgtggt tctcaaccac aaggctgctg ctgatcacag ggaagccttt caagttattg
    27841 aagtggatcc tgtagaccgt acagttgaac ttggagaacc cttcaccatc aatggctgga
    27901 ctagttttac cttcgatggt cgccaagata cctacaatga cttccactgg cactggtacc
    27961 acttcacagg tacagactat gatgccaaac gccgtaagtc tgggatttat ctgatccaag
    28021 gggacaacaa gggctgggct aacgaggaat tggtcgataa cgaaaacgga aactacgact
    28081 acctcatgta tgccgaccta gactttaaac atcctgaagt catccaaaac atctatgact
    28141 gggctgattg gttcatggaa acgactggtg tagctggttt ccgtttggat gccgttaagc
    28201 atattgactc tttctttatg cgcaacttca tccgcgatat gaaggaaaaa tacggtgacg
    28261 atttctatgt ttttggtgaa ttttggaacc cagacaagga agccaatctg gactatctcg
    28321 aaaaaacgga agaacacttt gaccttgtcg atgttcgtct ccatcagaat ctctttgaag
    28381 ccagtcaagc tggcgcaaac tatgaccttc gtggcatttt cacagatagc ctggttgaac
    28441 tcaagcctga caaggctgtg acttttgtcg acaaccacga tacccaacga ggacaagccc
    28501 ttgagtctac cgttgaagaa tggttcaagc cagcagccta tgccctcatt ttgttacgcc
    28561 aagacggcct tccatgtgtc ttttacggag actactatgg gatttcaggc cagtatgctc
    28621 aagaagattt caaagaaatc cttgaccgcc tcctagccat ccgaaaagat ttggcctatg
    28681 gagaacaaaa tgactacttt gaccatgcta actgtatcgg ttgggtacgt tcaggtgctg
    28741 aaaatcaatc cccaatcgca gtccttatct caaatgacca agaaaacagc aagtcaatgt
    28801 ttgtcggtca agaatggact aatcaaacct ttgtagattt acttggtaac caccaaggtc
    28861 aagttacaat tgatgaggaa ggttatggac aattccctgt ctcagctaga tccgtaagtg
    28921 tctgggcagt caataccatc taatagctca taataaccaa gctaggtcca agcggatttg
    28981 gcttttttgt attcacaaaa agacctaccc aaatggatag atctttactt gattacaatt
    29041 tacctgctac tgcatccaac aattcttgga tcttaggttg gttgcttcct cctgccatgg
    29101 ccatgtctgg tttaccacca ccacgtccat cgatgattgg tgctaattct ttgacaaggt
    29161 ttcctgcatg aaggtctttt gtcttgcttg ctacaaggac attgactttg tcaccgatag
    29221 cggcaactag gacaagaaga tcagagtagt ctttttgttt ccagttatct gcaaaggtac
    29281 gaagggcacc tgcatcggat acagacactt gactagcaat gtaacgatga ccgttgactt
    29341 ccttaacatc tttgaagata tcgcctgcgg ctgcagctgc ggctttttct ttcaactcag
    29401 cattttcttt ttgaagttga cgaagttgtt cttgaagtcc ttctaccttg tgaggtactt
    29461 ccttgacttg aggtgctttc aaggttgctg cgacagcttt aagagcatcc tcttgttcac
    29521 gataggcttc aaaggcttcc ttaccagtca ctgccaagat acggcgagtt cctgaaccga
    29581 ttccttcttc tttgacaatt ttgaagagac caatctcaga agtgttgcca acatgagtac
    29641 caccacaaag ttcaatagag tagtcaccga tagtcacgac acgaacttcc ttgccgtatt
    29701 tctcaccaaa gagggccata gctcccattt ctttagcagt gtcaatatcc gtttcaactg
    29761 tcttcacttc aagtgcttcc caaattttct cgttaacttg ctgttcaatc gcacgaagtt
    29821 cctcagcagt tactgcttgg aagtgggtaa agtcaaagcg aaggaattca acttcgttaa
    29881 gagatcctgc ctgtgttgcg tggtttccaa ggatattgtg aagggcagcg tgaagcaaat
    29941 gagtcgcagt gtggtttttc atgacacggt gacggcgatt gctatcaatt gccaaggtat
    30001 attcttggtt caaggcaagc ggtgcaagga cttcaactgt atgaagggct tgaccatttg
    30061 gggctttctg aacattggtc acagtagcca caatcttacc tgactcatcc aagatttgtc
    30121 cgtagtcagc tacctgtcca cccatttcag cataaaatga cgtttccgca aagataagag
    30181 aggcagttcc ttctgaaaca gctcctactt ctgcattgtc agcaacgata gctaccaatt
    30241 tagaagacaa ttggctagta ttgtagttga agacactttc tacagtgatg ttttgaagag
    30301 tttcattttg catacccatt gagccaccct tgacagctga cgcacgcgcg cgttcttgct
    30361 gttctttcat ggctgcttca aaaccttcac ggtctacagt cataccagct tcttcagcga
    30421 tttcttcagt caattcaact gggaacccat aagtatcata gagtttaaag acatcttgac
    30481 cagcaatgac agattgacct ttttctttca agtctgccac aatgccttgg gcaaagtgtt
    30541 gacctgagtg aagggtacgg gcaaatgatt cttcttcgct cttaacgatt ttctcaataa
    30601 agtcacgttt ctcaagcact tctgggtagt agctttccat gatttttcca acagttggaa
    30661 ccaatttgta aaggaaaggc tcgttgatac ccaatttttg accatgcata gaagcacgac
    30721 ggagaagacg acgaagaaca taaccacgac cttcatttcc tggaagggca ccatcaccga
    30781 tagcaaatga aagagaacga atgtggtctg cgataacctt gaagctcatg ttgtcgccat
    30841 cttggtcata aaccttacca gacaatttct cgacttcacg gataatcggc atgaagaggt
    30901 ccgtttcaaa gttggtctta gccccttgga taacggccac caaacgctcc aaaccagcgc
    30961 ccgtatcaat gttcttatgt ggcaattcct tgtattcgct acgaggaaca gcagggtctg
    31021 cgttaaattg tgacaaaacg atgttccaga tttcaatata acggtcgttt tcaatatctt
    31081 ctgcaagcag gcgaagaccg atattttctg ggtcaaaggc ttccccacgg tcaaagaaga
    31141 tttctgtatc tggtccagaa ggtcccgcac cgatttccca gaagttgtcc tcaattggaa
    31201 tcaagtgact tggatccact cccacttcaa tccagcggtt gtaagaatct ttatcgtctg
    31261 gatagtaggt catgtaaagt ttttcagcag ggaaatcaaa ccattcaggg cttgtcaaaa
    31321 gctcataagc ccaagtgata gcttcgtcac ggaagtaatc cccgatagag aagttcccca
    31381 gcatttcaaa catggtatgg tgacgcgcag tcttccctac gttttcgatg tcgttggtac
    31441 ggatagcctt ttgggcattg gtaatacgtg gattttcagg gataatggtc ccgtcaaagt
    31501 atttcttaag ggttgctacc ccagagttga tccacaaaag agttgggtca tttacaggaa
    31561 ccaaacttac tgatggttct actgagtgac ctttggtcgc ccagaaatca agccacattt
    31621 ggcgtacttg tgcactagat agttgtttca tattgtctcc ttattcactt gtttaatgtg
    31681 attggctttc cagcatttcc acatagtcaa tcgcgacaca gagggaaatg actaggtctg
    31741 cataagcgtc ttcaagaacc gttacggtat aggtagaggt cagatggaag agttccttct
    31801 taatttccgc aatcaactga tcgcgatcat ccagcaattt gaaattcaaa tcccagatat
    31861 tgccctcgat acgaagacct agattatcaa actcatactt atctcgccag aaggtcaact
    31921 tcttacgaat gacaaaactc gagccatccc gaagctgaat ctcaaaacga ggaagcaagg
    31981 tcaagatttc tttactgatc tgactgactt gttcaccagc cgcatcatag atggtaaaag
    32041 ttttgggaat cttaaaaaat gatccctcca cctgataggc aatttctccc ctgtcatcct
    32101 tgatagcgaa gcgttcgcct ccaagacgaa acttttgttt gacaagaaat gttttcatca
    32161 acacctccaa aaatcaaaag acaagctcat atcacgaagg gcgaaaaacc gcggtaccac
    32221 cttcattcaa tgaacttgtc attctcttgt tcttatgcaa ttgtatgatt gagtagcatg
    32281 acttcctagc ttagatggct cgcagcaccg ccatttctct ggactaagac aagtgaaaat
    32341 caattctcaa ctttcttatt ataacgtttt tttaagcttg cgtcaactgg aaatgatctc
    32401 cgttgaatta gaccaattcc ctacatctct gattactttt tcaggatata ttttttctta
    32461 ctgccatttt tctttttatc ccaaattttc atattactaa acacagctac tagaatattt
    32521 ccaaatataa aggtgcctat cacccaatat atggactcag ttgttaggta ttgtcgatcc
    32581 aagccatcat ttaaatggaa tagtatagca gtttggttaa caatcataaa ggttggccag
    32641 aaactttttt tgaaaaaagt agacattttc attatttgtt gccgctttct gtaaggttaa
    32701 tactcaataa aaatcaaaaa gcaaactagg aagctagcct caagctgtac ttgagtacgg
    32761 caaggcaacg ctgacatggt ttgaagagta taggcttagt atactactag gcaagcaaat
    32821 aagcaaataa gcaaataagc aaataaacaa ctagaataga aaaagatagg gctctaaaaa
    32881 ctgacttcta ttccttaaaa acgaaccagc ttgactgatt cgtcttctta cgtttatctc
    32941 ctacttccga tacattttaa actgtaggaa gaggtcgcta tatttccctg tccatttatg
    33001 gtcaaatttc tcataaactt ctaggtgttt catggtttca acatcgggat agaaggcctt
    33061 atcttccttt gtttcctctg ggagcaattc cttcgctggt aggtttggtg ttgaatagcc
    33121 gacatactcc gcattttgga gagcattttc aggtttcaac ataaagttga taaaggcata
    33181 ggctgagttt tggtttttaa ctgttttggg aatgaccata ttgtcaaacc aaagattgct
    33241 ggcctctgtc ggtaccacat aacgtagatt ttcatttttt tctaacattt ggctggcttc
    33301 accagagaag gtcacgccga ttgcaacatt attctgaatc atatagccct tcatctcgtc
    33361 cgcaacgata gccttgatat ttggagtcag tttgtagagc ttatccactg tctcttccaa
    33421 ctgctgcaga tccttggagt tgaggctgta gccgagggaa ttgagtccta gtcccagcac
    33481 ctcacgcgcc ccatcaaaga gcatgataga attcttatac tccggcttcc aaaggtcatc
    33541 ccaatgctca ggcgcttcat ctaccatggt ttcgttgtag acaattccta aggttcccca
    33601 gaagtaaggg atggagaatt tattacctgg gtcaaaggac tggttgagaa actctgatcc
    33661 gatattttcg attccttcaa tttttgaata atcaagcgga accaagaggt cttcgtcctt
    33721 catcttgtta atcatgtatt cacttggaat ggcaatatcg taggtcgttc caccctgctt
    33781 tatcttagtg tacatggctt cgttggagtc aaaagtctcg tactgaactt gaattcctgt
    33841 ttcttctgta aactgagtca agagttcagg atcgatatag tctccccagt tatagataac
    33901 caatttttga ctatctcgac tattgatttt actatctaaa tgagtcgcaa ttccccacaa
    33961 gacaaggata atcgctgcaa ttcctgctaa aaatgaatag atttttttca tgcttgctcc
    34021 tccttctcac gagagataaa gtaataacct acaactagga taatactaaa gagaaagact
    34081 agagcagaca gggcattgat ttctaaggaa atccccttgc gagcacgaga gtaaatctcg
    34141 actgataggg ttgaaaagcc atttcctgtt acaaagaagg tcacggcaaa gtcatctaac
    34201 gaataggtga aggccatgaa ataaccagta atgatagacg gagtcaggta aggaagcatg
    34261 atttccttga acatctgaaa ttgactagct cccaagtcat aggccgcatg aatcatgtcg
    34321 ccattcattt ccttgagtcg aggcaagacc atcaagacca cgataggaat ggagaaggcc
    34381 acgtgactag atagaacggt caaaaagcca agtgaaaact tgagttgggt aaagagaatc
    34441 aagaagctag caccaatcat aacgtcaggc gcaaccatga ggatattatt gagtgataga
    34501 aaggcttctt ggtatttctt acgagactgg tagatgtaaa tggcaccaaa agtcccgata
    34561 atggtcgcta tcaaggctga taggaaggcc aagaaaaatg tctgagccaa aatcagcatg
    34621 agtctcccat ctccaaacat ggtttcaaag tgagtccagc taaaacctgt aaagctattc
    34681 atatcatcac cagcattaaa ggcatagcca atcaagtaaa agataggcag gtagaggacc
    34741 agaaagacca gtcccagata aaggttggca aattttttca tcgttctctc ctttccttag
    34801 tcacccacat ggtgatgaac atggtcagga tgagaatcac accgatggtt gaacccatac
    34861 catagttgtc attggttaga aaattctgct caatagccgt ccccaaggtg ataacgcggt
    34921 tcccaccaat caaacgggtc agcatgaaga gactcaaact tgggataaag accgactgaa
    34981 ccccacttcg cacaccgttc atagatagag ggaagatgac atgacggaag gtctcccact
    35041 tagttgcacc aaggtcataa ctagcattga tgagattatt atccatatcg tctaagacat
    35101 tgaaaatcgg caaaatcata aaggggagct cgatgtagct tgcgacaaag ataaaggaaa
    35161 aatcggtaaa aagcaactgt tgtgaaccaa ttccgataaa ttccaagaat tggttaatag
    35221 agccattttg accaaaaatc ccgataaaag cataggcttt aaggagcaaa ttgatccagg
    35281 taggaaggat aatcagcatg agccagagtt gacggtgctt gagacgggtc aaaaagaggg
    35341 ctgttggata gctgataagc aaggtcacaa aggtcacaat ccctgcataa agcactgagt
    35401 tgaaactcat tttgaggtag gtcaagtttt gtgacgcaaa gtaagattta taattttcta
    35461 aactaaactg gccttcaatg ttgaaaaagg attgtccgaa aatcaagacc aagggtgcca
    35521 atacaaagag ggcaatccaa agcatgtagg gcactacaaa gagtttagag cttgttttct
    35581 tcatctcttt cctcctcgat cgcattgatc agacctgctt cttgctcttc gatttccacg
    35641 tattcttcga tacgagcatc gaactcttct tcggtttcat tgagacgcat gatgtggatg
    35701 tcttctgatt caaagtccag accgatttcc tcacccacga tggccttacg ggttgagtgg
    35761 atcatccatt catttccaag ttcgtcatag gcgataattt cataatgaac tccacggaag
    35821 agctgggtgt cgaccttaac ttggagtttg ccttcttcag gaagggtaat gcgcaagtcc
    35881 tctggacgaa taacgacctc aacaggttca tttggcttca tcccaccatc aaccgcttca
    35941 aagcgtttgc cgttaaattc gaccaagtag tcctcaatca tggtacctgg caagatgttt
    36001 gactccccga taaaggtggc aacaaagtgg ttgattggct catcgtagat gtccacaggg
    36061 gttccagact ggacaatctc gccatcattc ataacgaaaa tccagtcact catggcaaga
    36121 gcttcttcct gatcgtgagt gacaaagaca aaggtaatgc ccaatcgttg ttgtaattca
    36181 cgcaattcgt actgcatgtc tgttctcaat ttcaagtcca gcgctgataa aggctcgtcc
    36241 aacaagacca cacggggttg gttgatgata gcacgggcga tggccacacg ctgacgttgt
    36301 cctccagaaa gtttgcggat ggaacgtttt tcataacctt ccaactgaac catcttgaga
    36361 acttccgcta cgcgttgctc gatttctttc ttgtcaatct tgcgcaagcg aagtggaaag
    36421 gcaacatttt caaacacatt catatgtggg aacaaggcat aggattggaa gacagtatgt
    36481 acgtcgcgct tgttggttgg aatatcattg atacgaacac cgtctagcat gatatctcct
    36541 gtcgtcgcat ccagtaaacc tgcaataatg tttaggatag ttgatttccc cgaaccagat
    36601 gcacctagaa gggtgtagaa tttcccttct tccaactcaa agttgatgtc tttgagaacc
    36661 ttggtgttgc tgtcttcaaa aactttagag acgtttttga attcgataat tggttttttc
    36721 aattggcata aattccttct ttttcataga ttaaccgatc ggggctctgt caggtcccca
    36781 ctacctcttg cagggagtaa aaccacctgc atacatcttc gctaccgata ggctttcacc
    36841 cccctttcca tgacatagca gcagcttttc aaatacagct tcctacttgc tttcacccaa
    36901 gatccggact tctctttcaa gcgtaatacc tgagtgttcc ttgacttttt cgataaccga
    36961 ttggatcaag tcctcgtagt ctttggccgt tccatctgcg acattgatca taaatcctgc
    37021 atgcttttct gacacttcta cgccaccgat acgatagcct ttcaagccag cttctgaaat
    37081 taactgacct gcaaaatgcc cgactggacg cttaaagacc gagccacaag atgggtattc
    37141 caaaggttgc ttgagttcac gtaggtgcgt caagcggtcc atttcctgct tgataacctg
    37201 atgggttcct ggggctaggg caaatttaac tgacaagaca actgcaccag actcctgaat
    37261 agctgaatgg cggtaaccaa aagccaagtc tttagcagac agggtttcga tttctccatc
    37321 cttggtcaag accttacaag actgcaatat gtgagcaatc tcgccaccat aggcacccgc
    37381 attcataaag acagcaccgc caacgcttcc tggaatacca caagcaaact caaagccagt
    37441 caaactatga cggagggcaa tgcgagttgt ttcaatcaag ttagccccag cttctgcttc
    37501 aatggtatag ccatcaacag aaacgttatt gagcttgtca cacaagatga caaatccacg
    37561 aatcccacca tcacgaacga tgatattgct tgcattgcca agaaccatcc aagggatatt
    37621 ttcttggttg gcaaatttca caaggcgagc caactcaaaa cgatttcgtg gaaagaccaa
    37681 ataatcagcc tctccaccta cttttgtata actatagcta tgcaagggtt cctgaaaacg
    37741 gatatcaatt ccttctaaga tttcaagcat tttttctctt acagacatgt cactcttcct
    37801 tttacaaaat tcattccatt ataccatttt tagagacatt tgacgaccat aaaaatacct
    37861 tgtttggatt ttgcataaga aaaagaggtt cccctctttt tatgattttt tacaaaagat
    37921 ttccttggtt ccataggcga ccagaacgag ctccagtgct agaatcactt caaccaagac
    37981 tggatttgtc aaccagccta cttggaaaag agatggtgcc agatcaaaga aggcatgcaa
    38041 gccataggct gttaggagat aaatccattt cttctggcga acagcttgat aaacccaaac
    38101 tgtcaaaagt aattggaaac caagcgccaa gattcgctca aaaccaagca aataaatctg
    38161 ccagaccgaa agtgactgaa tggtttttaa catattttca gacagtaatt gcataacctg
    38221 tggattctga gtttgaactg ccgaaagaac aatgtaaaga ttgagtaaac tagtaaggcc
    38281 tagaaaaatc aactccaagc caccatgccc caatccatag gccaaggcgt ctgccttttc
    38341 caaacttctc tttttctcca accatttgaa gaaaacaaga cgagccgttt cctcaaaaaa
    38401 tgctaccatg gctaggccat agataatata gataagcgga tgaacttaca agagggcaat
    38461 actaccgtct ttttgaggat gcaaaatcaa gatatgcacc agtttttcca aaatctgcga
    38521 agagacaaag aaagcgactg cacccaagtc caagacagct agattaatct ggtatttctt
    38581 tttggcaaac caaatgcttc ctatcagaaa agctagcaac agcaacatgg taataatgat
    38641 atgaatggtc attttcttct cctattctgc cttttcaata tcttttttca tctcgtcaac
    38701 attgaactta gcaaacaagt attgacggtc ttggactggg aaacgttggt ccaactggtc
    38761 aactgctccc acctcgataa tgccttcctt gatgacaaaa tccatgacat gtccaccgaa
    38821 ggtcaaatca tctgatatga agtgcagatg gtagcctgcc acactgaccc catggaaaat
    38881 ctcaggcgtc cagaaaccaa caatagtccc cgcaacattg tcacgactat attccggttg
    38941 atgggttgcg acatcagcaa acttggtatc gggtgttgac ttaggaatca tacgcacatg
    39001 catatgtgaa aattcccccc gaatcttgat agagcggaaa agattttccc catcataata
    39061 agactcaatt cgttctttca attccttgtc tgtcatctca aagcgctggc ggaaaatgac
    39121 ctctgcctga tgcggtacca ctgcagcgta aggaataagg gcatctggtg acacttccac
    39181 aatttctggc tggtctcctg accctttggc ctgataagcc ttgccatcca agacaatcaa
    39241 ttccccatca atggaatcca aggttcccaa accaaggtca ccatgttcta gcaattctcc
    39301 tactgtcata gtaccaccat aaaggccagc catcaaggct ccaagagtat tgtattgaaa
    39361 taatttcact ggttcctgca cgttcttatc cattcacttt cttgtctgtt attctataaa
    39421 tcttactcct aagtatacca catttgcccc tagatgtgaa cgagagaaac gctctagaca
    39481 ttgccaagaa ggaaaaacag ggtacaatgt aacaaaatca agggaggtct ggaatgaaga
    39541 aacaaagcaa gtacaaagag gtcgtttcct atctgaaaaa tggtatcgag tctggacgat
    39601 ttccgacggg tagcctgaga ttttgacaga aacaaaactt gcactagcac aacaacccct
    39661 aaggaacaca cttccctagg ggtcgctttt tatttaacta aatcattatt tattttttgt
    39721 tcctagtaaa cataaatatt gtattaataa ttgcaaaaat aaatgcaaac aaaaggattt
    39781 tccaaaggat aaatttttgt aacactaaat atgcatatac aaatccaatc aaaaagctaa
    39841 taatcccatc ttttattaaa aagtttttat cacgcattat tccagaccac actgaactgc
    39901 ccaaatagcc aactgaccag caatgatata aatatttgtt ttaagtccat tagctttaac
    39961 aaatcttagt accactttag ctaattctga ccaagccttt ttctgcgcat acttaacaat
    40021 tagcgaaaca ttgatcatag caaaaaattc atctcgtatt tttcctgcta tacatcctcc
    40081 catctcatcc caagggactc tgtcataggg attattaata ttagtttcta attcattttc
    40141 cgcaagagca atatcttttt gagaaaagtt tttataaaca tattcagtaa aatttttttc
    40201 attaactcca tttctaaaca tttgttcaag aacattggct acatcatcga tttgtttttg
    40261 ttcttgggta ctaatttcag aaacttccgt agtagttaca tcatcagcaa atactctaac
    40321 agaagctata gaaggagaca taatagaaaa tagtgataat acaacaagag ctgaagttac
    40381 aattttttta aatttcataa aaaacactcc tttcaagtca caaatatata ttactgataa
    40441 aataacttga ttttctgatt taaaactcct ttctaacaag ttattcgtat gcttttattt
    40501 tttagttcta ttataccaca acaagaaaac gatttcaagc attttcctaa aaatatgtac
    40561 aagtaatatg tacaagtaat aaggggaaat tagcataaaa actttttaaa acaaatcaac
    40621 cccagataaa aatacacatg ttgttttaaa gacaaaaaag ggtacgaaaa actgtcacat
    40681 ctgcatttga ttgctctttg atttcaaaaa agtattaaaa atgggagtat accacatttg
    40741 cccctagatg tgaacgagag aaacgctcta gacattgcca agaaggaaaa aaaagggtac
    40801 aatgtaacaa aatcaaggga ggtctggaat gaagaaacaa agcaagtaca aagaggtcgt
    40861 ttcctatctg aaaaatggta tcgagtctgg acgatttccg acgggtagtc gcctgccttc
    40921 tatccgtcaa ctgagccttg actttcactg cagcaaggac accattcaac gagccctgct
    40981 ggaattacgg cacgaacaat atctctatgc caagcctcag agtggctact atgtattaga
    41041 acaagggcaa catcaagacc tagaaatcga ggttaccgac gaacatgcca gtgcctatga
    41101 cgatttccga ctctgtgtca atgaaacctt gattggccga gaaaactacc tcttcaacta
    41161 ctatgacaat caagaaggat tagaagacct aagacagtcc attcacaaac tcctctttga
    41221 gcaagctctc tactgcaagg ctaaccaact agtactgact tctggaaccc aacaagcctt
    41281 gtttatcctc tctcaaatat cctttcctag acaagccaag gaaatcttgg tggaacagcc
    41341 aacctaccat cggatgaatc gcctcttgat tgcacagggg ctggactatc aaacgattga
    41401 acgaggcatt gatgggattg acttggagga gctggaaggc cacttcaaaa caggaaaaat
    41461 taaatttttc tacaccattc ctagatttca ctatcctctg ggacattcct attctgagca
    41521 ggacaaacga tctattctta acttagctgc caagtatgat gtctatatcg tagaggacga
    41581 ttatctgggt gatttggact ccaagaaggg ccaaaccttc cactatcttg atacagagga
    41641 gcgtgtcatt tatatcaagt ccttctcgac cagccttttt cctgcccttc gtattacagc
    41701 actcattctt ccaaatgcta tcaaagaagc atttgtggcc tacaaaaata tcctagacta
    41761 cgacagcaac ctcattatgc aaaaggccct gtcactctat attgacagtc aattgtttga
    41821 aaaaaatcgt ttggctcgct tgaccaatca tgaatcttac caaaaacaaa tcgaggaaag
    41881 gataactaaa acaccttgtc cccttcctca ttatccccta cacgatggtt tattgctaga
    41941 cctgagacag tatcctaaaa tcgccagtct caaacacagt caactgggct tggacttctt
    42001 tgaagaggcc tatttaagca cctgtcctta tcaatttgcc aaggtgtccc tagacaatct
    42061 ggaaaatgtt ttaaactatt taaaagcaga attggaatga catccaactc tgctttttct
    42121 tattcttcaa cttcagttaa acttgctgct ttttcaagat agctttggta agttccttca
    42181 tccttaagtt tttggatgac cttgtctacc acttctttca aatcatcact attttttcta
    42241 agagcaacgg cattggcgtc gccgtccttc atcttcaagc tgacagttgc gacagctaag
    42301 ccagcgtttt tagcagcata actaagtgca acaggctcat ccatatgaac agcatctact
    42361 tttccagcct gcaattcatt gactgcttca cccatattag ttagggaagt taattgagct
    42421 tttggcaatt gttccttgac cattgattct ggaacagtcc ctttttgggc tgcaatatta
    42481 gcactttcta ggctagttaa atccttgtat ttttccacat cagccttacg aaccaagaaa
    42541 ctaatcttgt tttcatagta tgggattgaa aaatcaaaga cttcttttct ctcgtcagta
    42601 gcactaattc ctgcaactgc taggtcagcc ttaccagttt gaagactggt caaaacatta
    42661 tcaaaactca tgcttgagat ttccaactta accccaagtt catcagcgat agcctgagcc
    42721 atgtcgatgt ctgcaccgac tacctggttc tttccatcaa ccaatgattg aaattcaaag
    42781 ggtgcatagt caggactggt tgccacaact aatttcccct tttgcttaat ggcctcaaca
    42841 gctgactgag aaccattaga acctgactgg caagctacta agaagaaact tgcaagaaaa
    42901 ttacataaaa caaatatcca tttttttgat ttcataaata aacaacctct ctgttaattt
    42961 tgtataatta taacgatatc caagttactt gtcaagtgtt ttttaaattt ttatctcaaa
    43021 aatatttttt cgttcaaaaa aaggagccat cagttgattt caagctcctt tttatacaga
    43081 attaaactat tttatagttc gacaatctta cctgtttcaa agtagacaac ccattcacag
    43141 atatttttag catagtcacc gatacgctcc aagtaggaaa taacttggaa ataatcacga
    43201 cccgtaacaa tggcttctgg atttttctta atctcttcag tcgcaaggtc acggatagtt
    43261 tcaaaatagt ggttaatttg ctcatccatg gaggccaccc ggtatgcgtc gtcaacagaa
    43321 ccattaagat aaagatcaag tgctgcttca acaacgcttt taacttcacg tcccattttt
    43381 ttaatttctt cctctacagc tggaatgcgc tcttccccct tcatacggat ggttgcctga
    43441 gcaatggcta cagcgtgatc ccccatacgc tccacatctg atacagcctt aaggacagtc
    43501 aagactgtac gcaaatcttg agagactggt tgttggagtg cgatcatttc aaatgatttc
    43561 ttttccagtt tcacttcgta ttcatttact tctgcatcat cttcgatgac ctcttttgcc
    43621 aggtcacggt catgcgtgac aaaagcacgt accgtacgat tgatttgtga gagcacttct
    43681 tgtcccatag cgtagaactg gttatgtaat ttctctaaat cttcttcaaa ttgagatcgt
    43741 aacatctttc atctccttat ccaaattttc ctgtaatata gtcttccgtt tccttgtgtt
    43801 ggggattaag gaacatctgc ttggtatcat taaattcaat caaatctcca tctaggaaaa
    43861 atcctgtctt atcagagata cgtgaagctt gctgcatgga acgggttacc agaagcatgg
    43921 tgtacttgtc ttttagacca tacaaggttt cctcaatttt accagctgaa atcggatcca
    43981 aagccgaagt tggctcatcc aagaggatga ttttaggact agttgccaag acacgggcca
    44041 cgcagacacg ctgctgttga ccacctgaca atccaatagc tgaatcatat agacgatcct
    44101 tgacctcatc ccagatagag gcaccttgca aggctttttc tacggcttca tccagaacct
    44161 gcttatcctt aattccattg atacgaagcc cgtagacaac attctcatag atagtcatag
    44221 ggaaaggatt aggttgttgg aaaaccattc cgatttcctt acgtaattca accgtatctg
    44281 tacgcggact gtagatgttg tgaccattgt acaccacgga tccagttgtg gtcacctctg
    44341 gattgagatc tcccatgcgg ttgagagact tgaggagggt tgacttccct gatccagatg
    44401 gaccaatcaa ggctgtaatt tccttaggtt ggaaagatag ggaaacacta ttcaaagcct
    44461 tctttttatt ataataaacg gacaggtctg atacctgtaa aatcgcatct gtcatacggt
    44521 ttcctttcta accaaagtga ccagatacat agtcattggt ggactgtagc ttggcatttt
    44581 ggaaaatagt tgcagtcttg tcatactcaa tcaaatcacc caagtaaaag aagcctgtat
    44641 agtcacttgc acgagcagcc tgctgcatat tatgcgttac aatgatgatg gtaaagtttt
    44701 tcttgagctc aaacatggtc tcttctagtt gcatggtcgc aatcggatcc aaggctgagg
    44761 ctggctcatc cattaagagg atatctggct taacagagat ggcacgagcg atacagagac
    44821 gttgttgctg accacctgat aaggtcaagg ctgacttgtg gagatcgtct ttaacctgat
    44881 cccaaagggc agcctgacga agggaggttt ctacgatttc atctaggact tgcttatcct
    44941 taactccagc acgttcatgc gcaaaggtaa tattacggta aattgactta gcaaatggat
    45001 tggggcgttg aaaaaccatt ccaatgtgtt tacgcatttc ataaacgttg atttctggac
    45061 ggttgacatc aattccacga tagagaatct gcccagttac tttagcaata tcaatggtat
    45121 cattcatgcg attgagactg cgtaagtagg tagatttccc cgatcccgac ggaccaatca
    45181 aagctgtaat tttatttctt tcaaattgca tatcaatccc cttaatggat tcatttttac
    45241 catagtaaac atggacatcc ttagtagaaa gggctacttt ttcttcagga aaggtaagga
    45301 tatgcttctc atcccagtta tatgttgaca tggcttctcc tttaggcagc ggttaatttc
    45361 ttgtgtagat agcttccgaa cttacgagct ccaaagttaa aaatcaggat aaagatcagg
    45421 agcacagcgg cagaacctgc tgatacaatg gttccatctg gaatagtgcc ttcactattg
    45481 actttccaga tatggacagc caaggtttct gcttgacgga agatagagat ggggctagtc
    45541 acactgagga tattccagtt agaccagtca agagctggcg ccgattgccc tgctgtatag
    45601 atcagagctg cagcttcgcc aaagatacga ccagatgcca aaacgacacc cgttacaata
    45661 cctggaagcg cttctggaat aacaacatga accactgtct cccagcgaga aatcccaaga
    45721 gccagaccag cctcacgttg ggtatggtga acgtgtttca aactatcctc tacattacgc
    45781 gtcatctgag gcaagttaaa gactgtcaag gccaaggcac ctgaaatgat tgaaaatcca
    45841 tactcaaact ggactacaaa gatcaagtaa ccaaagagac ccaccaccac tgatggtaaa
    45901 gaggacaaga tttcaataca agtccgcaca aagttggtaa caggaccttt tttagcatat
    45961 tcagccaagt aaatcccagc tcccatagaa agaggtacag aaataatcaa ggtaatgacc
    46021 aataggaaaa aggaattgta aagctgaatg ccaatcccac cacctgcttg aaaagcagaa
    46081 gaccttccag tcaagaaaga ccaagagata tggggcaagc cccgaaccaa gatatagaga
    46141 atcaaggaag ccaagattgt cacaatgatg ctagcaatcg tatagaggac agctgttgca
    46201 agtttatcta atttcttagc gcgcataatt tttctttcct ctttctttcg taatcaattt
    46261 aatcacactg ttaaaaacta aactcatcaa gagcagtacc aaggccagtg accagagaac
    46321 attattattt acagttccca tgacagtgtt cccaattccc atagttaata tagaagttaa
    46381 agttgcagct ggtgtggtca aggaagttgg gataacagct gagtttccga caaccatctg
    46441 gatagctaga gcctcaccaa aggcacgcgc catcccaaag accactgcag tgaaaatacc
    46501 agaacgggcc gccttcaaga tcacacgcca gatagtctgc cagcgagtgg ctcccatagc
    46561 gaaactggct tcacgataat aacgaggaac cgcacgcaag ctatccgttg tcataaaggt
    46621 tacggtcggc aaaatcatga caaagaggac ggaaatccct gacaaaatcc caaaaccagt
    46681 cccaccaaag acactgcgaa caaagggaac gacgacttgc aagccaataa atccgtacac
    46741 tactgaagga atcccaacca ggagttcaat agctggttgc aaaatcttcg ccccttttgg
    46801 tgatacttcg gtcataaaaa ctgctgcacc aatagcaaag ggtgttgcga taagggctga
    46861 gagaatggta acgataaagg aacccaaaat cataggaagg gcaccaaatt ctttactaga
    46921 aggattccaa gttcctccca aaagaaagtc aaagatattc acaccattga caaagaaggt
    46981 cgacaagcct ttttgcgcta cgaaaaccaa aatcatggcc acaaggatga ctatcaaaga
    47041 aagacaggca aaggtcaaac cttttcctaa tttctccaga cgagaattct ttgatggaag
    47101 caacattttc ttagctaatt cttcttgatt cattattgtc tcccttccaa cactgtcaca
    47161 gttccggcag catctttttc aaccttcatt tccttaatcg gaatatactt caatcctttg
    47221 acaatccctt cttgggtctc atccgagaga acaaaattga gaaattctgc agccaactca
    47281 ttgggctgcc ccaatgtata catatgctca taagaccaca agggccaatt attgctactt
    47341 atattttctg gacttaagtc atagccattc aacttcatgc ttttgaccga atcatctata
    47401 taggtaagag ataaataaga gatagctcct ggactttttg atacgattga ttttaccgct
    47461 ccatttgaat cctgctcctg actttgcatg gcagactgac cttccataat gacagtatca
    47521 aaggtagcac gagagccaga gccggctgcc cgattgataa cagagatggg taagtcctta
    47581 ccaccaacct ctttccaatt ggttacctca cctatgaaga tttgacgaag ttgctctgtc
    47641 gttaggttat caacatcaac ctccttattg acaatcagag ccaagccagc taccgcgacc
    47701 ttgtggtcaa caagagcaga agcatcaatt ccgtcttttt cctcagcaaa tacatctgag
    47761 tttcctatat caactgcccc agactgaacc tgggacaagc ctgtaccaga acctccccct
    47821 tggacattga ccgtttttcc aacatggatc gtgccaaatt catctgccgc tacttcaacc
    47881 aagggttgca aggcagttga gccaacagcc gttatggatt ctccacgatc aatccagcta
    47941 gcacagccta ctaaacaagc cgtcagccaa aaagcgataa gagacagagc aagctttttt
    48001 ctttttttca ctgtttttct cctcgaaaat aattatgaat actgtgaatt ttttaagtag
    48061 ttctttatga gttgacgcat gaattcttac caaatttctg cgcaattgat tatttatata
    48121 atataggcta tattactctt tcctaacctc cttttttcat atgtggataa aatctcttgt
    48181 ctatcccttc ccccattgtc acccattata gtcatttcgt gtctcttttt ccccttttta
    48241 atgcaaggga aattactctt cttagatgat aatccaaaag ctagaaaggt atctcaaacc
    48301 tctctactct cccagactag tttacaacta aaaggaaaag attctatttt atgagaaatc
    48361 tagtttacaa gcggtaagaa cgctaataac taaacttctt gtactctttg aaaatctctt
    48421 caaagcagtg ttttgagcta tctatggcta gcttcctagt ttgctctttg attttcatcg
    48481 agtagtaaaa ctacatgtga tggcaatcaa gatatcaaga atcatcctac taaaaaaatc
    48541 catactttca ctataacata gaataagata tttgactagc attttcattt gaatctgagg
    48601 ccttttggaa aataattttt caaaacattt ccagtaacct ttgcaaagcc caagccattg
    48661 cctttaacca aaacttggta ccaaccattt ggcagacttt ctgccagctg aacggtttct
    48721 ccagccgcat acttgacaaa cgcttcttgg ccaatttcaa ccgactgttc gacctgactc
    48781 ggtttcaagg ctaaaccaag agcgaaactg ggctcaaagc gtttcttctt aaaagtaccc
    48841 agatgcagtc cattgcgagc aatcttgagc ttccctaaat ctggcaaaag ttctggcaag
    48901 agataaagct ggtctccaaa aatctgcaag atacccggta gattgacctt caaatggttt
    48961 tgggcaaatt cctgccacaa ggcaacttgt tcacggctga ggttactctt acttgcctta
    49021 aatttaggag ctggattgtt acccttaaac tgtagatggg caacaaactg accctctccc
    49081 ttaaactgat gagggtacat ccgagccgtt tctggcaggt caataccagc taccattcca
    49141 ttgatatgct ctactggcaa caagtcaaaa tcatactctt ccagcaacca attgacaatc
    49201 tcttcgtttt cctcgggtgc ccaggtacag gtcgaataaa ccagacgacc accttcagct
    49261 aacatggtca ctgcatcctc cagaatttct ctttgcaagc tagcacattg actcggataa
    49321 tctaagctcc aatagtccat agcatcaggt tgcttacgaa acattccttc accagagcaa
    49381 ggggcatcaa gaacgattaa gtcaaaatag cctttaaaga ccttgaccaa gcggtcggca
    49441 gattcattgg tcaccacgac atttgtcgct ccaaaacgct ccatgttttc aaccaaaatc
    49501 ttagcccgtt tgcttgaaat ttcattggaa acaagtagcc cctcccctgc tagataggct
    49561 gccagttgag ttgatttgcc ccccggtgca gcagccaagt ccaagacctt cataccagga
    49621 ctgggttggg ctacttgagc caccatttga gcagcaggtt cttgcgaata aactaaacct
    49681 gtagcatgct caggcgattt ccctgaaacc ttcccatagt ggccccaagg ggtttgagta
    49741 atggcatcag aaaaggaaag ttgctcttct tttaagggat tgacccgaaa ggccgaaacc
    49801 gcttcctcct caaaagaggc aagaaaatct cttgcctcat ctcctagtat ttctttatat
    49861 ttttcaacaa atccttctgg aaatttcatt taagttcttt tcctttcgta aatataggac
    49921 tgaatttcct cctgcatctc aagaggcacc atcatgaccg gctgtctggt ttgaaaatca
    49981 ggagcttcac caaaaagggt cacaacccga tagcccagac tttcccctaa aatactagct
    50041 gcggcataat cccatggttg cagataggtg agataggtca acaaatgccc tgacaaaatc
    50101 ttggcaaaac taatggccgc acttccatag acacgaacac caagaaccgc tcggctcaaa
    50161 tcagccagcc cccattcatt ggtttccagc ataccactat tccctgcaat gagaaaatct
    50221 ccaagtggtt tagttttaaa aggagctagg ggcctatcat ttagacaaac tggaaattcc
    50281 ccaccaccgt ggtaacaatc ccctttgacc acatcataaa tcagaccaaa ctgtccctga
    50341 ccattttcaa aataagccat cataacagca aaatcttcct gctgggctac aaaattattg
    50401 gtaccatcaa tgggatcaat gacccaaacc ttgccctctt gaaccgaggc tcgcagacaa
    50461 ccttcttcag cacaaatctt atcctcagga taacgggaca aaatctcacc aaccaagagt
    50521 tcctgaactt ctttgtccag tctggtcacc aaatctgttg gagaggactt ggtttcaaca
    50581 cgcaagtctt cctgcatatg gtcaagaatg tactggcctg ctttcttaac aagctcttta
    50641 gcaaattcaa atttactttc caagagaaat ctttccttcc cctttttctt tggcagcctg
    50701 aactgctcgg taaagtgaat agccactaac cgtttcaaat tctcgtccca aacgtttctc
    50761 ttcgctcttg cttggcacga ccgctttgaa tcccttatag gagtccagta actttttagc
    50821 ctctactttg ccttcatagg cagcttcaac atcattaaaa aaagaaagca ctgaagcaag
    50881 ttcttcagtg ctccacgaca aatctagtgg gtaactatac tgtttgttca ttaactaata
    50941 ccagctctca ttcttgcttc ttttagttct tgcttacgat aactacgagg gagaaaagca
    51001 cgaatctcat cttcattaaa accgatttgc atacgcttgg catcaataat aattggacga
    51061 cgcaaaagac taggatactg ctcaatcaaa tgaagcaatt ccgataccga aatactctct
    51121 acatcaatat tcaatttttg aaaaattttt gaacgagttg aaatgatgtc atcagtacca
    51181 ttttcggtca aggaaaggat gtgttgcaat tcttttcttg ttaaaggact ggtcataata
    51241 ttgtgttcca caaagggaac cttatgtttt tctaaccagg ccttagcctt acgacatgat
    51301 gtacagctcg gtgatagaaa tagtgtaatc atgcttttct cttcttatct atactttgct
    51361 acttctatta tacaaaaaaa taaagcgctt gactagggat ttttagaaaa aaagcctatt
    51421 ttttcaagaa aaataggctt tttgcgaacg attgacacaa ttggatttgg ttaattcact
    51481 cttaacgatg gttttaaacg atatatattt ttatatatgt aaattaaaaa catctttcct
    51541 ttcacttcct acgacttttc agatacagat agccaaagaa gttttcatag agggcaaaaa
    51601 agaggaggaa ggcatgaaga aagaaggtct ctggcaaaat cataataaca ggatccttgg
    51661 ctggatcaaa aagccaggta tcatctccca caaagagaat ttgatggaaa agagtaaaga
    51721 attggtcaaa accaatcaaa actcccccaa gtccaatcat cacaggtaag actactagag
    51781 ccaggagact ttttcgataa agagacaaaa agtccttttt cacaatccta ttgacaaaga
    51841 catagaaact tggcagtgtc actagagcta ctagctgaac caaatgaaag agattcttga
    51901 ccactgcgaa atggtgcaga ccagctgctg acgaacgaaa atcaggcatc tgtaagacct
    51961 gactaaaagg attggtcaga taattcatca agatatgaaa attgtattga atggtttctg
    52021 gttttagata gactcgattc gttaagttta gccactgaat ctccatagga tagaaaatcc
    52081 aagccagata aatggtcaga aggattgaga gggagaggag aaagagcata gagccccaaa
    52141 aggtcaattt agttttcatc aaaatcccac tccgcaaggc tagaaaccac atgtgtcggt
    52201 gcgattggca ggccagctac ttcttctgcc ttagtaaaac ctgtcgtcac caagagcgtt
    52261 ggaatgccat tgtcaatccc agcccggata tcagtcaaat aattgtcccc aaccatgatt
    52321 aactcttcac gttccaaacc taagtgctca accgccttgt ccataatgat ggcatttggt
    52381 tttccgatat aaaccggctt cactcgtgtc gctacttcaa gcagcgtaat cagtgagcca
    52441 gcacctggca aaagaccacg ttccgtcggg atgttgaggt caggattggt tccgataaaa
    52501 tgggcaccct tttgaatagc aagagttgct gtggcaaatt tttcatagtc gacttgccaa
    52561 tccagaccaa ctaccacgta ggcagggttt tccttgtctt ccacataacc agccgccttg
    52621 atggcttcct tgagtcctgc ttctccgacg acatagacag tcttttcaag ccccaaatca
    52681 ttcatatagt cgatggttgc caaagtcgct gtgtagacag tcgatagagg cgtatcgata
    52741 ttaaaattct gagccaacat ctccttaaca ctctctggag tgcgggttgt attgttggtt
    52801 acaaagagat agggaatgtc ccgcttttgc aattcatgaa caaaagtctc tccagcaggg
    52861 attcggtctt tccccttata aatggttccg tctaaatcaa ttaaatagcc tttatatttc
    52921 atctatttct ccctaaacct tttttatttc ttgccaagta atgattgctt gggcattgat
    52981 aaccccatca cttgtaattt catgcttgct ttccagtcca gtccgttcaa cagccgatgt
    53041 aatcacccca cctggtcgaa cttccttgac atacttgagg ttgattttct tgggaatata
    53101 gtgggtcaaa aaatccgctc ccatgacctc aaaaatccag tccaagtatt tactgttatt
    53161 gacatgacca ttcatatcca agtcgtaaaa acgaacatgg taatccttgc tgatcggctc
    53221 ttccaaggat tcatacttcg gtccacggat aagtttttta tcaaaatcag actggtaagg
    53281 agccacaatc tcaggttcaa cagcatggac ttttcgactg tcgcggtcca tgagaacaaa
    53341 ggtcgccatc atatggatga gctcctgccc cgcttcatta taaatagtaa agcgacggta
    53401 gcaaaaaagt cgattgtagc tcaaggcttc cgtttcgatg gtaatttctt ccgcaaaacg
    53461 aggcaaacga accacctcaa tatcatattc tgcgataatc cagaccagat tatagtcttc
    53521 caaaatggcc ttatcactaa ctcccagttc aatcgattgc atccctgaaa cttgcagtga
    53581 cagcaaaatc acatctggaa gtttgatatg accgttcata tcagccatat caaaaggaat
    53641 tttcattttc atttgataag ttaagcacat gatcctactc caaaataaat cgttctgcta
    53701 cagtatctcc caaaaagaga cctctctttg tcatgcgaac gtggtcaccc tcaatctgca
    53761 tgaggccttg ttgaaccaaa tctctgacaa tttctccata aagtccagca aaagactgtc
    53821 caaatttttc ctcaaatcgc gccatggaaa ccccggattt cttgcggagt cccaagaaca
    53881 tttcttcttc catttgctcc ttttgactca ggtgatcttc tgtaatacaa gcattgcctt
    53941 cctcaaccgc actgagataa tgacgaatgg gaccatgatt tttatagcgt actccattga
    54001 cataaccaga tgcccctgca ccaataccat agtattcagc attgtcccag tacatgagat
    54061 tgtgacgact ttcaaagccg ggtttggaga aattagaaat ctcataatgc tcaaaacccg
    54121 ctcgctccag ctctgcaatg atgtactcaa acatctccgc ttctagttcc tccttaggca
    54181 gaggcaattt cccacgtcgc atccggttca taaagaccgt atggttttct aaaatcaaac
    54241 tatacaaact catgtgggga atatccagtc caatggcttt agccacattt tcctttactt
    54301 gctccatggt ctgaccaggc agagcataaa tcaaatcaat ggagatgttg tcaaaaccag
    54361 ccagtttcag gcgatcgata ttttcataaa tatccttctc caaatgactg cgcccaatct
    54421 ttttcaacat cttatcatca aaggtctgga cacctagcga aacacgattg acagccgaat
    54481 ttttcaaaac agctatctta tccgcatcca aatcgcctgg attggcttca atggtcaact
    54541 cttccaagac agacaagtcc aagtttttag tcaagccatt cagtaacacc tccagttgcg
    54601 gagccgacag ggctgtcggt gttccaccac cgatataaag ggttgacaac ttttcaatat
    54661 cataagaacg aaactcttcc agcagatgct ctaaatagct gtcgactggc tgatttttga
    54721 tgaagacctt tgaaaaatca caataataac aaatctgggt acaaaatggg atgtgcacat
    54781 aggctgacgt tggttttttc tgcatagtaa ttattatacc acaaagacta gattccagat
    54841 aaaaatcacc atccccagat acatagtccg tccggagatg gtgatggttt attcttctgt
    54901 tatatcaatc acaatctctt ctgagtcatc aagagcttcg gctttttctt gccattgctc
    54961 cttgagatta tttaattgat tttttgatgc ttctgtcgct tgaaaagcat aggatttagc
    55021 ttgagcaagt atactgtcca cagtgatttc acctgactca acctgttctt ttgttttcag
    55081 aacaaaatct gtagcctgct ccttaacttc tgtcagtttt tcacagactt gctccttggc
    55141 atactccgga tcttctctca aatcatctag aaaatcttga gcctgactgc aaacttgttt
    55201 gcccttatca cttgttaaaa acaaggcaag agctgcacct gaaacggttc ctaaaaggat
    55261 tgaggataat ttacccataa ggattctcct tttttatttt ttgaaaaatt tacttgcaag
    55321 acgaagagct gacagacttg caccagtctt gagtgttttt gaaccagctg atgaagcttt
    55381 cttgctcaag acacgcgcat ggtcattgag gtctgaaaca gatagagata aatctgcaac
    55441 agcactgaag agtggatcaa tcgtagccac cttgacattg atatcatctg ccaagacatt
    55501 gaccttagcc aacaactcat tggtgtgatg caaggtcaca tccacatctg aagtcaaggt
    55561 tttaatcgtc ttttctgttt catcgatgac acgaccaagc ttttgtacag taatgatcag
    55621 atagaccaaa aagacaatca aagctagggc aacaagaata tatgcaactt ctaacattta
    55681 gttttcctcc tctgtaatat agtaaggggc cttctttcga ttttgataaa taacgatcat
    55741 tataccgaga ccgataaaga caactgacag ccattgggac actcgaaggc tgaagaacat
    55801 gagactatct gttcgcatac cttcgatgac catacgaccg aaaccatacc aaatcaagta
    55861 aaaggccgtg atatgacctc gtctgagact cttccatttc cgtctaaaaa tcagaatcaa
    55921 ggcaaagcca agcagattcc atagagactc ataaaggaaa gtcggttgac ggtagctccc
    55981 ctcaatatac atctggtcac ggataaagcc aggtagataa tccagattat ccactgttgc
    56041 accataagct tcttggttaa agaaattacc ccaacgcccc aaactttgag caatcataac
    56101 gctaggcgcc gcaatatcta gaaaatccca agtattgatg agtttacggt cagcaaagat
    56161 atagagcaca agagccccag ttatcaaacc accgtaaatg gccaaaccac cattccaaat
    56221 ggcaaaaatc tctcctaaat tctgactata gtaatcaaat cggaaaataa catagtagag
    56281 acgagctcct aaaatagcca agggaaaggc tactaagata aaatctaaaa tatcgtctgg
    56341 tatgatcttc tttctaggtg cttctttcat ggtcaaataa accgcaagaa tcaagcctgt
    56401 cacaatacat aaggcatacc aacgaatggc taggggtcct agttgaatag caattggatc
    56461 aagcattttg cacctcattt cgagctatga gacttgtcag tcgttcgtcg aacaaacggg
    56521 tagcatcaaa gcccatttcc ttggcacgat aattcatggc agctgcctca atcacaacag
    56581 agatattacg acctgtttta actggaatac gaatacgagg aatggctacg ccagaaactt
    56641 caagttcctc tgcattgttt ccaagacgat caaaggtctt atgcgtatcg taattttcca
    56701 aatagacagc aagctgaacc tgtgaagaat ccttgacagc actcgcaccg tagagactca
    56761 taacatcgat aataccaacc ccacgaattt caatcaagtg tttcaaaatt tcagctggtt
    56821 caccccagag agtaatctca tccttggcaa agatatcgac acggtcatcg gctaccaaac
    56881 ggtgaccacg tttgacaagc tcaagacctg tctcgctctt accaattcca ctatctccct
    56941 gaatcaagac gcccatccca taaatatcca tcaagacacc gtgcacactg gtacgttctg
    57001 ccaaacgaga atccagatag ctagataact ctccagataa acgactggta gctgcacggc
    57061 tggttaaaat agcaatctta cattctctag cagcctttaa catctcctct ggaaccacca
    57121 aaccacgggc aacaatgact gctggtgtct caggtagaaa catttttttc aaaacttcat
    57181 aacggctgtt ggaaggcatg ctgatcagat aagaccactc cttcatcccc aaaagttgaa
    57241 tccgctctgg tgtatagtag tcaaaatagc ctgtcatttc aagaccaggt cgcgtaatat
    57301 ccgctgtatt gatttccttt tcaagcaatt ctggttcacc atagacaata tctagtctaa
    57361 gcttttcaat cacttctttt actaaaaccg acatcatttc ctccttcaaa tgaattcttc
    57421 tctctattat atcaaattgt aggaggaagt ttcagatttt tgcaggcttt ggagtattta
    57481 tttaaaagaa aaaggactag atttctctag cctttcattt caattagaat tttttacgtt
    57541 tacgagctac ttctgattta cgtttacgtt ttacagaagg tttttcatag aattcacgtt
    57601 tgcgtgtttc ttgaagagta ccagctttag taaccgcacg tttgaaacgg cgaagtgcgt
    57661 cgtcaagaga ttcattctta cgtactactg ttttagacat ttttttcact cccttcaagt
    57721 tcaatatcta tttcattcta cacgtaaaag gaataattgt caagcaaaaa tgcggtttaa
    57781 ttttgatttt ttcttccatt tatacaagag ttgaaagcgt tcaatagaac attgcttttt
    57841 tatttttcga gtaagctaag cgcttcagca tctgcgatga tggttacatc agggtgattt
    57901 tggaggctac ttgcaggtag gttctcagtc actgggccag atactgttcc ggcaatggct
    57961 tctgctttcg actcaccata agcaaaaaga ataatagact tggcatccaa aatgttttta
    58021 atccccattg aaatagcttg ggttgggacg tcttcaatct tggcaaagaa gcgagcattg
    58081 gcttcgatag tagactggtc aagttctact agatgcgttt gactgtcaaa tggagtacca
    58141 ggctcattaa agccgatatg cccattgcga ccgattccca agatttggag gtcaactgga
    58201 tggtcagcca aaatttggtt gtagcgttca acttcagctt cagcattgtc cttaacacca
    58261 cgaggcaaga aactttcttt aaatggtttt tggttgaaca agttttcttg catgaagtaa
    58321 cgataagact gtggattgtc cccatcaagg cccacatact catcaaggtt gacactggtt
    58381 agatttgaaa aatcaaggtc actctcaaca atttccttgt aaaattcaag tgggctactt
    58441 cctgtcgcaa gtcctagagt ttgagctcca ttagccaatt tttccttcaa aatctcaaaa
    58501 gcaacttttc caccttgaac ttggttttca actttaataa ctttcatctt ttcatcctcc
    58561 atttctgtct atatttatta tatggtatag accaattttt gtcaagtgaa aacgatttaa
    58621 tttctaaaaa aagagcgtcc aaacttgaaa cgtgttataa tggataggat tagaacttag
    58681 aaagaatgaa caaatatatg aatacagctg attttgattt ccacttgcca gaggaattga
    58741 ttgcccaaac gccccttgaa aaacgtgatg cctccaaact cctcatcgtc aaccgtgaga
    58801 caggagaaat gcaagataaa catttccact ctattattga tatgctggaa cctggtgatg
    58861 cccttgtcat gaacgacacc cgagttctcc ctgcccgcct ctatggtcaa aaagtggaga
    58921 caggaggtca tgtggaactt ctcctcctta agaacactag tggagacgag tgggaagttc
    58981 tggctaaacc tgccaaacgc ctcaaggtcg gtactcgtat cagctttggt gatggccgcc
    59041 tcagcgctgt cgttacagaa gaattgaccc acgggggacg cattgtccgc tttgaatacc
    59101 aaggaatttt cctagaagtc ttggaaagtc tgggagaaat gcctctgcca ccttatatcc
    59161 acgaaaaatt agatgaccgt gaacgttatc aaaccgtcta cgccaaggaa agcggctctg
    59221 ctgcagcacc gactgctggt cttcacttca ccaaagaact gctggcagaa atccaagcta
    59281 agggtgttca tctagtctat ctgactctcc atgtcggact cggaaccttt agacctgttt
    59341 ctgtggataa tctggacgaa cacgaaatgc actcagagtt ctatcaactt tctgaggaag
    59401 ctgctgccac ccttcgctct gtcaaagaaa atggtggtcg cgtcatcgct gtcggaacca
    59461 cttctatccg caccttggaa actattggtt ccaagtttga tgggcaaatc caagcagatt
    59521 ctggttggac caatatcttt atcaaacctg ggtatgagtg gaagatcgtg gatgccttct
    59581 caaccaactt ccatctgcca aaatcaactc tggtcatgtt ggtttctgcc tttgcaggcc
    59641 gtgaattagt cttagatgcc taccaccatg ccatccaaga acactaccgc ttcttcagtt
    59701 ttggtgacgc catgtttatt tattgagaaa gaatttctct aattcttcta ataccaataa
    59761 atcgctaaga tattatttca aagaacatct acaattgaaa ctctagctag ctgtagaaga
    59821 cgcctagtac attgaaatta aaatgcttcc ccctagcttc gaaaatattg ccatagattg
    59881 cgttgactct ccaaattgat tcatctatat tttatttcag cttcctatat tttcttcgct
    59941 gtttgtaaat caaaatgcaa gacacatgag tagcaccata tttgttactc ttatctgtcc
    60001 tctcaagaga ctattatgag ttatttcaga atcattcact actttgaccc tgactctcct
    60061 tagtctcaaa atcaaagact tatactcttc aaaaatctct tcaaaccgcg tcaacgtcac
    60121 cttggattat atatgtgact gacttcgtca gttctatcta caacctcaaa gcagtacttt
    60181 gagcaacctg cgactagttt tctagtttgc tctttgattt tcattgagta ttaaacaaaa
    60241 agtgaacaaa tctgaattct aatgtacaga agactaggct tgttcacttt tttatagtcg
    60301 ctataagatg accttatcta tagcttttta tatataatta tatattcaga catactatta
    60361 tcaattttgt cgcagggagg aatctgttaa cgcacccatt caccattatc attgactcta
    60421 tagccatcta tacttgtatt gaccgctaac tcacccgatg catttacata ataccattta
    60481 ccaccaactt ggaaccattg attgactttc atagaaccgt tgctgttgag gtagtaccat
    60541 gaaccattaa cttgtaccca acctgttgcc atggaaccat cagtattata aaaataccac
    60601 ataccatttt cttgtttcca gtctgttgtt ggagcaactg ctttagctgg ttctactgct
    60661 acatctgttc cttggttaga tgtaacagat acaggatacg aaggaataga tgattgctca
    60721 ggaacaacaa ctttttcagg ttctctcgtc cctctcctta tacgtctttt taccatctct
    60781 ttagtaattt gacgagaagt agtttcttca attattccat cacgttcatc tacagtatag
    60841 attgtagtaa gagtaattta ccaatttctc ctacttcttc tacttcttga cttttatcaa
    60901 gagttgggcc atcgagatat tctgtttcga ttggaatttc ttggacaaga acttggggct
    60961 tggttccttt tttaacaact cttgtttgag agtctttttt tgacttaaag tactctcagt
    61021 tacttgtcca ctctttccat ctacattata agttatcgtt gtaactgttt tcccattctt
    61081 tcctagagta atctcttgct cctgtcctgc agaaaggtca ttgtctgctt catatttagt
    61141 agcaaatgga acaagaactt cttcaatctt gcttttagct ggaactttga taactgtatc
    61201 cgtggcttct tttctatcaa cagtaacctg ttcggtaaca taaccagtct ctggattaac
    61261 atcgtaggtc cttgtcgtag ttacatagcc atcctctccg tcaattgtaa caggattttc
    61321 actacggtct tttgtttcat ctttttcaaa acgaattcgc gtacttgaaa ttttcttggt
    61381 tactacctta ggtttagtcg ctacttttac aataatatcc ccattgtcag cgtcatcata
    61441 ctctattccc tcttctttat ctctagtatc atctctgaca tattgaatcc catcagcagc
    61501 atgagcaaaa cttgtattca gattcctcct aaaaataaag ttagcccgat tatcgcagaa
    61561 ccaaaaatct ttccgagttt acgtattgca taacgcttat tagtattaga ttttgccatt
    61621 acatcctact tctagtatag catcttttct atcaaacgtt aaacaatata ctttatatat
    61681 aaaatagact tagaatgata tattgattat tgaactaaca ctttaactat atcgtaatca
    61741 atctcatata taaaggattg cagacatctg atctaaatac atgctaatat atttagatac
    61801 aaacattcca acttgataat tttcactcat ctttcaacat tccttacaca acgatgcagt
    61861 ataaatagaa tagttttctc atcagaatga gactatttta atattagata atttgataga
    61921 aaaacataaa taaaattggt tgaatttaat tccctaactc gatagaggat tctattttaa
    61981 atttaacaac agctaggatt acactataaa aagtacatta ccacctgttc ggctggagaa
    62041 tctagtcgat tagctccctc ttagttacaa gcttaaagcg attttttaac taatactaaa
    62101 ccagattaaa gaaggaacta gaacatctgt cttgtactaa tcaatatgct attttaaaat
    62161 tgcaaataaa cttggaatgc taattattat atttacaata aatagttgac ttcgatagaa
    62221 acagaaaata gaattcaaca ttcttcccta cttataccat aataaataag atccgcaaga
    62281 tagccttttc tctctacacc attaacaatg gtttgtagat aggacattcc agccttttcc
    62341 atgacacgac ctgaagctgg gttgagactg gcataacgtg ctctgacctt ttgaaaacct
    62401 gcttgagtaa aacaaaagtc caagacagct ttcaaggcct ctgtcatcat accatgtccc
    62461 cagtaagcct tgcctagcac atagccgatt tcacagctta aatcagcctc gtctatctta
    62521 acgatgctga tatctcctat cacttgctct gggttttctt ttagacaaat ggcccatttg
    62581 taatagttga gattagtata ggaagcaacc caattgcgaa tcgagtttcg agtgatttcg
    62641 acatcaggat ggggatccca ggtaacatag gtcagattct cagtggatga agcccaattt
    62701 tgaaacatgg cttctgcatc actctccaca aatcttctta aaatcaaacg ttctgtctgt
    62761 aatatttgcg taccgataga tttcatgatg ctacctcact tccttatttc tatactcgta
    62821 gtaagaaggc tcaactcagt aacagctaga aaacgagttt gtcttctaat ttcttgacgc
    62881 agttgctgcc tggtttgaaa tgcgctctag caagcttttt caaacctagt catcgttgca
    62941 ggggtaagac gatgaaatcg aactcttcga gttactgaat ctgcctcatt ttcttatttc
    63001 taaactcggg gttcaagatc ccccagacct tctctcactt tcttatttca agatgagagg
    63061 ctgaaaacct ccactggagg tttttcttgc tttttaattt cagggcaaga agctaaaaag
    63121 gtcccccgga ccttcttcgc tttctcattt ctaggctcag ggtaaaaacc tccagtggag
    63181 gtttttactc ccaaaagtca tcaaatacag tgatgggtaa gtggcgtttg tgttggcctt
    63241 tgtgccacca gttttcaatg gtcgcttgag cttctgggct gattgttttg ccttctaggt
    63301 agtcgtcaat ctctgcgtag gtgactccaa gtgcgacttc gtcagctagg cctggtttat
    63361 cttcttctag gtctgccgtt gggatttttt cataaagggc tggctctgcg ccaagtttct
    63421 gcaagagctg ttttccttgg cgtttattga ggcggtaaag agggagaata tccgcaccgc
    63481 cgtcaccaaa cttggtaaag aaacctgtga tattttccgc ggcgtggtct gttccaatga
    63541 ccgctccgct atgggaacca gcaagggcat actgagcaat catacggcaa cgtgccttga
    63601 tattcccctt gttgaagtct gaaacaggac tacctgtcgc ttcaactgca gctgtcatgg
    63661 catcagctga ttccttgata ttcacaacca agctgacatc tggctggatg aaggctaggg
    63721 ctttttgagc atctgcttca tcagcttgca ctccgtatgg caggcggaca gcgataaatt
    63781 tgtagctatc gtctcccgtt tcagctcgca gttcttccat agctaattgc gccaaacgtc
    63841 ctgccaaggt tgagtcttgt cccccagaaa tccctagtac aaaggttttt aggaagggat
    63901 gttttttcag atatcttttt aagaaatcaa tagaacgacg gatttcttcc tgggcatcaa
    63961 tcactggttt gacacccagc tcttggataa tcgtttcttg caaactcatt cttcttctcc
    64021 ttcaccaagg gcttccttgc gcatcttatc aatcaagtcc atcttatctt gccatacatc
    64081 acgcgccaaa tccactggat agtgctgcgg attgagcaca cgcttatact catcccacaa
    64141 cttgtcaaat tccttacggg cataatcctg aatgtcagtc aaactaggca agttgtaaac
    64201 taatattcct tctttgaaga tatccaccaa gagaggaacg gcatcaaaat tacgaaccgt
    64261 cttcttgatg tatgtatagg tcggatggaa catcttgatt tctgtcatgt cgctaatatc
    64321 cacaccatca taagtgatgt agtcaccttc tgacttgcct ttttcacgac tggtaatgcg
    64381 ccacacctgc ttcttacctg gcgtcgacac tttttccgca ttattagaca gcttaatcgt
    64441 attgcgcatc tggccgtttt catcttcgat tgcaacaatc ttgtaaaccg ccccaagagc
    64501 cggctggtca taggctgtaa tcagcttggt acccacaccc cagacatcaa tcttggcctt
    64561 ttgcatcttg aggttaagga tggtattttc atctagatca ttagaagcat aaatcttagc
    64621 ctctggaaat ccagcctcgt ccagttgctg acggactttc ttagaaatgt aggcaatatc
    64681 cccagagtca atccgcacac ccataaagtt aatctgatca cccagctcac gcgccacctg
    64741 aatggcagct ggtacaccga tgcgaagggt atcataggta tccacaagaa agacacaatt
    64801 tttgtgggtc gcagcgtaag ccttgaaagc ctcatagtca ttgccataaa cctgtaccaa
    64861 ggcatgggca tgggttccca aaacaggaat gtcaaagagc ttacccgcac gcacgttgct
    64921 ggttccattg gcgccaccaa tcaccgctgc gcgtgttccc cagatggccg catccatttc
    64981 ttgagcccga cgtgtcccaa actccatcaa gggttcatct tcgataaccg aacgaatacg
    65041 agctgccttc gtcgccacca aggtctggta gttgacgatg ttcaaaagag ccgtttcgac
    65101 caactgacat tgggctagag gtccttccac ctgcacaatc ggttcattag caaaaaccaa
    65161 atccccttct tgggcagaac gaacggtcaa ctccaacttg aaattgcgaa ggtaatccaa
    65221 gaacgcccca tgataaccaa gcgactccaa ataggctata tcactatctg aaaaacgcaa
    65281 gtcttcaaga tagttcacaa ttctttccaa acctgcaaaa accgcatagc cgttcttaaa
    65341 aggctgttgg cggaaataca cctcaaagac cgccttctta ttgtaaatcc cttgatcaaa
    65401 gtaaacctgc atcatgttga tctggtacaa gtccgtgtgc aatgtcaaac tatcatctgg
    65461 atacatactt ttcctacttc cttagctaga aacccatgaa aattttcaag aactttcatg
    65521 tattccaata aattagtact attatatcac attttagctg gattgagaaa agagtaacaa
    65581 gctattctcc actctccaat tcatccatat cttgttcaaa ttttttctga gcccattcgc
    65641 catagctctt aagaccaaga ttgccaataa agacccacgg aaggtaaatg acataagtaa
    65701 tgacccaagc agacaggtat ttaaaattca aaggattgtg ctgataaatt tctatgttga
    65761 attgataatt ctgcaacatc aaaagagccg taatagccaa ggttaggaaa aaacaaccca
    65821 aaatcgtaaa atgaaaacga ctatagtagg tcactcccag ataacgggca cgattgaaaa
    65881 agtaaaatgt ccctatgatg ataacgatta gcagcatatt agaattaaaa aggcttggtg
    65941 ctaatactga aatgatataa gataggagcg acaaagcaat gcagatatag aaactttcag
    66001 agcccgcttt attgaacagt tgttcttctc tttcgtctag taattgataa taataaaatc
    66061 tatttttcat cttcttcctc ccaaaatagt tggtctaggg ttttccctaa acatctgcaa
    66121 atagactggc agagcgagag actgggattg tattttcccg cctctatcaa accaatagtc
    66181 tggcgtgtca ccccgacagc ctctgccagt tgaccttgtg ttaaatcacg ctctacccga
    66241 gctaatttta attttaaatt tttagccacc ttcgtcctcc ttatagtttt aatactcatc
    66301 tactcttaaa aaatccaaaa ccaacacaag ctatcagcta tttattataa ccccattaat
    66361 ttgcactcca gtattatctt cagccgaaaa tcagtatttt aggttgtttg aaataaactt
    66421 cctagtttac tcttttattt ttatttagta ttagctttag taggatcttt taaacgagtt
    66481 tttattgctt atatttcttg tttagtttag ttg
//
LOCUS       DAVKQB010000004        65366 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKQB010000004 DAVKQB010000000
VERSION     DAVKQB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 65366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65366
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.004"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..1174
                     /locus_tag="VW528_000213"
     CDS             <1..1174
                     /locus_tag="VW528_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9911103.1"
                     /translation="NLIKLLIIRLIVNLADSVFYIVALWHVSNNYSSSMFLGIFIAVN
                     YLPDLLLIFFGPVIDRVNPQKILIISILVQLAVAVIFLLLLNQISFWVIMSLVFISVM
                     ASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDSIFNSFASFLQVAVGFILLVK
                     IDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSISLT
                     LINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQIV
                     GVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGRVN
                     TTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY"
     gene            1599..1832
                     /locus_tag="VW528_000214"
     CDS             1599..1832
                     /locus_tag="VW528_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9911104.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            2115..4064
                     /gene="mutL"
                     /locus_tag="VW528_000215"
     CDS             2115..4064
                     /gene="mutL"
                     /locus_tag="VW528_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9911105.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(4389..4856)
                     /locus_tag="VW528_000216"
     CDS             complement(4389..4856)
                     /locus_tag="VW528_000216"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9911106.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIAVAWIPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4857..6062)
                     /locus_tag="VW528_000217"
     CDS             complement(4857..6062)
                     /locus_tag="VW528_000217"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9911107.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(6082..6717)
                     /gene="ribE"
                     /locus_tag="VW528_000218"
     CDS             complement(6082..6717)
                     /gene="ribE"
                     /locus_tag="VW528_000218"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9911108.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(6702..7802)
                     /gene="ribD"
                     /locus_tag="VW528_000219"
     CDS             complement(6702..7802)
                     /gene="ribD"
                     /locus_tag="VW528_000219"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9911109.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7937..8051)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8208..8801
                     /gene="ruvA"
                     /locus_tag="VW528_000220"
     CDS             8208..8801
                     /gene="ruvA"
                     /locus_tag="VW528_000220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9911110.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8811..9374
                     /locus_tag="VW528_000221"
     CDS             8811..9374
                     /locus_tag="VW528_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9911111.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            9371..10048
                     /locus_tag="VW528_000222"
     CDS             9371..10048
                     /locus_tag="VW528_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9911112.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIATYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVDYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(10203..11234)
                     /locus_tag="VW528_000223"
     CDS             complement(10203..11234)
                     /locus_tag="VW528_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9911113.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKVLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEEPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDNNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(11489..12172)
                     /locus_tag="VW528_000224"
     CDS             complement(11489..12172)
                     /locus_tag="VW528_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9911114.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(12184..13128)
                     /locus_tag="VW528_000225"
     CDS             complement(12184..13128)
                     /locus_tag="VW528_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9911115.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            13260..16091
                     /gene="uvrA"
                     /locus_tag="VW528_000226"
     CDS             13260..16091
                     /gene="uvrA"
                     /locus_tag="VW528_000226"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9911116.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            16084..17145
                     /locus_tag="VW528_000227"
     CDS             16084..17145
                     /locus_tag="VW528_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9911117.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLFPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            17277..17675
                     /gene="spx"
                     /locus_tag="VW528_000228"
     CDS             17277..17675
                     /gene="spx"
                     /locus_tag="VW528_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9911118.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            17741..18310
                     /locus_tag="VW528_000229"
     CDS             17741..18310
                     /locus_tag="VW528_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9911119.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLVGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            18397..18663
                     /locus_tag="VW528_000230"
     CDS             18397..18663
                     /locus_tag="VW528_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9911120.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            18667..19086
                     /gene="ruvX"
                     /locus_tag="VW528_000231"
     CDS             18667..19086
                     /gene="ruvX"
                     /locus_tag="VW528_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9911121.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            19102..19407
                     /locus_tag="VW528_000232"
     CDS             19102..19407
                     /locus_tag="VW528_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9911122.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            19820..21070
                     /locus_tag="VW528_000233"
     CDS             19820..21070
                     /locus_tag="VW528_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9911123.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSCREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            21067..21624
                     /locus_tag="VW528_000234"
     CDS             21067..21624
                     /locus_tag="VW528_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9911124.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQP
                     TDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKI
                     YTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            21770..23302
                     /gene="cls"
                     /locus_tag="VW528_000235"
     CDS             21770..23302
                     /gene="cls"
                     /locus_tag="VW528_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9911125.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGINLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            23380..23523
                     /locus_tag="VW528_000236"
     CDS             23380..23523
                     /locus_tag="VW528_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911126.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            23560..25098
                     /locus_tag="VW528_000237"
     CDS             23560..25098
                     /locus_tag="VW528_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9911127.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFEFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            25218..27425
                     /gene="nrdD"
                     /locus_tag="VW528_000238"
     CDS             25218..27425
                     /gene="nrdD"
                     /locus_tag="VW528_000238"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9911128.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            27437..27577
                     /locus_tag="VW528_000239"
     CDS             27437..27577
                     /locus_tag="VW528_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911129.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            27621..28127
                     /locus_tag="VW528_000240"
     CDS             27621..28127
                     /locus_tag="VW528_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9911130.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNLASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            28120..28710
                     /gene="nrdG"
                     /locus_tag="VW528_000241"
     CDS             28120..28710
                     /gene="nrdG"
                     /locus_tag="VW528_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9911131.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            28707..29324
                     /locus_tag="VW528_000242"
     CDS             28707..29324
                     /locus_tag="VW528_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9911132.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQASMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            29590..29898
                     /gene="rpsJ"
                     /locus_tag="VW528_000243"
     CDS             29590..29898
                     /gene="rpsJ"
                     /locus_tag="VW528_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9911133.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            30115..30741
                     /gene="rplC"
                     /locus_tag="VW528_000244"
     CDS             30115..30741
                     /gene="rplC"
                     /locus_tag="VW528_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9911134.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            30766..31389
                     /gene="rplD"
                     /locus_tag="VW528_000245"
     CDS             30766..31389
                     /gene="rplD"
                     /locus_tag="VW528_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9911135.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            31389..31685
                     /locus_tag="VW528_000246"
     CDS             31389..31685
                     /locus_tag="VW528_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9911136.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            31703..32536
                     /gene="rplB"
                     /locus_tag="VW528_000247"
     CDS             31703..32536
                     /gene="rplB"
                     /locus_tag="VW528_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9911137.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            32640..32921
                     /gene="rpsS"
                     /locus_tag="VW528_000248"
     CDS             32640..32921
                     /gene="rpsS"
                     /locus_tag="VW528_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9911138.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            32933..33277
                     /gene="rplV"
                     /locus_tag="VW528_000249"
     CDS             32933..33277
                     /gene="rplV"
                     /locus_tag="VW528_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9911139.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            33290..33943
                     /gene="rpsC"
                     /locus_tag="VW528_000250"
     CDS             33290..33943
                     /gene="rpsC"
                     /locus_tag="VW528_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9911140.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            33947..34360
                     /gene="rplP"
                     /locus_tag="VW528_000251"
     CDS             33947..34360
                     /gene="rplP"
                     /locus_tag="VW528_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9911141.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            34370..34576
                     /locus_tag="VW528_000252"
     CDS             34370..34576
                     /locus_tag="VW528_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9911142.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            34601..34861
                     /gene="rpsQ"
                     /locus_tag="VW528_000253"
     CDS             34601..34861
                     /gene="rpsQ"
                     /locus_tag="VW528_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9911143.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            34887..35255
                     /gene="rplN"
                     /locus_tag="VW528_000254"
     CDS             34887..35255
                     /gene="rplN"
                     /locus_tag="VW528_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9911144.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            35333..35638
                     /gene="rplX"
                     /locus_tag="VW528_000255"
     CDS             35333..35638
                     /gene="rplX"
                     /locus_tag="VW528_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9911145.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            35662..36204
                     /gene="rplE"
                     /locus_tag="VW528_000256"
     CDS             35662..36204
                     /gene="rplE"
                     /locus_tag="VW528_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9911146.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            36222..36491
                     /gene="rpsN"
                     /locus_tag="VW528_000257"
     CDS             36222..36491
                     /gene="rpsN"
                     /locus_tag="VW528_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9911147.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            36776..37174
                     /gene="rpsH"
                     /locus_tag="VW528_000258"
     CDS             36776..37174
                     /gene="rpsH"
                     /locus_tag="VW528_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9911148.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            37366..37902
                     /gene="rplF"
                     /locus_tag="VW528_000259"
     CDS             37366..37902
                     /gene="rplF"
                     /locus_tag="VW528_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9911149.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            37986..38342
                     /gene="rplR"
                     /locus_tag="VW528_000260"
     CDS             37986..38342
                     /gene="rplR"
                     /locus_tag="VW528_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9911150.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            38360..38854
                     /gene="rpsE"
                     /locus_tag="VW528_000261"
     CDS             38360..38854
                     /gene="rpsE"
                     /locus_tag="VW528_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9911151.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            38826..39050
                     /gene="rpmD"
                     /locus_tag="VW528_000262"
     CDS             38826..39050
                     /gene="rpmD"
                     /locus_tag="VW528_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9911152.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            39195..39635
                     /gene="rplO"
                     /locus_tag="VW528_000263"
     CDS             39195..39635
                     /gene="rplO"
                     /locus_tag="VW528_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9911153.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            39648..40958
                     /gene="secY"
                     /locus_tag="VW528_000264"
     CDS             39648..40958
                     /gene="secY"
                     /locus_tag="VW528_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9911154.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            41109..41747
                     /locus_tag="VW528_000265"
     CDS             41109..41747
                     /locus_tag="VW528_000265"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9911155.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            41864..42082
                     /gene="infA"
                     /locus_tag="VW528_000266"
     CDS             41864..42082
                     /gene="infA"
                     /locus_tag="VW528_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9911156.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            42107..42223
                     /gene="rpmJ"
                     /locus_tag="VW528_000267"
     CDS             42107..42223
                     /gene="rpmJ"
                     /locus_tag="VW528_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9911157.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            42241..42606
                     /gene="rpsM"
                     /locus_tag="VW528_000268"
     CDS             42241..42606
                     /gene="rpsM"
                     /locus_tag="VW528_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9911158.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            42624..43007
                     /gene="rpsK"
                     /locus_tag="VW528_000269"
     CDS             42624..43007
                     /gene="rpsK"
                     /locus_tag="VW528_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9911159.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43050..43985
                     /locus_tag="VW528_000270"
     CDS             43050..43985
                     /locus_tag="VW528_000270"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9911160.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            43997..44383
                     /gene="rplQ"
                     /locus_tag="VW528_000271"
     CDS             43997..44383
                     /gene="rplQ"
                     /locus_tag="VW528_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9911161.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            44648..44914
                     /locus_tag="VW528_000272"
     CDS             44648..44914
                     /locus_tag="VW528_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9911162.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            44924..46261
                     /locus_tag="VW528_000273"
     CDS             44924..46261
                     /locus_tag="VW528_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9911163.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            46505..47197
                     /locus_tag="VW528_000274"
     CDS             46505..47197
                     /locus_tag="VW528_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9911164.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(47300..48945)
                     /locus_tag="VW528_000275"
                     /pseudo
     CDS             complement(47300..48945)
                     /locus_tag="VW528_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(48935..50026)
                     /locus_tag="VW528_000276"
                     /pseudo
     CDS             complement(48935..50026)
                     /locus_tag="VW528_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(50039..51160)
                     /locus_tag="VW528_000277"
                     /pseudo
     CDS             complement(50039..51160)
                     /locus_tag="VW528_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(51377..51574)
                     /locus_tag="VW528_000278"
     CDS             complement(51377..51574)
                     /locus_tag="VW528_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9911165.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(51649..52425)
                     /locus_tag="VW528_000279"
     CDS             complement(51649..52425)
                     /locus_tag="VW528_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9911166.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            52547..53293
                     /locus_tag="VW528_000280"
     CDS             52547..53293
                     /locus_tag="VW528_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9911167.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            53306..54286
                     /locus_tag="VW528_000281"
     CDS             53306..54286
                     /locus_tag="VW528_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9911168.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            54481..54801
                     /locus_tag="VW528_000282"
     CDS             54481..54801
                     /locus_tag="VW528_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9911169.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            54847..55155
                     /locus_tag="VW528_000283"
     CDS             54847..55155
                     /locus_tag="VW528_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9911170.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            55172..56470
                     /locus_tag="VW528_000284"
     CDS             55172..56470
                     /locus_tag="VW528_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9911171.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLAMSTMVYFPFFKIQDRLACQNEIKQS"
     gene            56621..59059
                     /locus_tag="VW528_000285"
     CDS             56621..59059
                     /locus_tag="VW528_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9911172.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            59078..59746
                     /locus_tag="VW528_000286"
     CDS             59078..59746
                     /locus_tag="VW528_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9911173.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            59764..60852
                     /locus_tag="VW528_000287"
     CDS             59764..60852
                     /locus_tag="VW528_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9911174.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            61334..63835
                     /locus_tag="VW528_000288"
     CDS             61334..63835
                     /locus_tag="VW528_000288"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9911175.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            64029..64724
                     /locus_tag="VW528_000289"
     CDS             64029..64724
                     /locus_tag="VW528_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9911176.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            64736..65152
                     /locus_tag="VW528_000290"
     CDS             64736..65152
                     /locus_tag="VW528_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9911177.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
BASE COUNT        19996 a        11746 c        14117 g        19507 t
ORIGIN      
        1 aaatttgata aagttgctaa taattagatt gattgttaac ttagcagaca gtgtatttta
       61 tatagtagca ttgtggcacg ttagcaataa ttattcttcg agcatgttct taggaatatt
      121 tattgcagta aattatctac cggatttgtt actaatcttt tttggaccag ttattgacag
      181 agtaaatccg caaaaaattc ttataatatc aattttggtt caattagcag tggctgtaat
      241 atttttatta ttattaaacc aaatatcatt ttgggtgata atgagtctag tgtttatttc
      301 agtaatggct agctccataa gttacgtgat agaagatgtg ttgattcctc aagtggtaga
      361 atatgataag attgtatttg caaattctct ttttagtatt tcgtataaag tattagattc
      421 tatttttaat tcattcgcat catttttaca ggtggcagta ggatttattt tattggttaa
      481 gatagatata ggcatatttt tacttgctct atttatattg ttgttgttaa aatttagaac
      541 tagcaatgcg aatatagaaa acttctcttt caaatattac aagagagaag tgttgcaagg
      601 tacaaagttt attttaaata ataaattatt atttaaaacc agtatttctt taacgcttat
      661 aaactttttt tattcatttc agacagtagt tgtaccgatt ttttctattc gatattttga
      721 tggtccgatt ttttatggta tttttttaac tattgctggt ttgggtggta tattgggaaa
      781 tatgctagcg ccaatcgtaa taaaatattt aaaatcgaat caaattgttg gtgtatttct
      841 ttttttgaac ggctcaagtt ggttagtagc aattgttata aaagactata ctttatcact
      901 tattttattt ttcgtttgtt ttatgtctaa aggagtcttc aatattattt ttaattcgtt
      961 gtaccaacaa atacctccac atcaacttct tggtagggta aatactacca ttgattctat
     1021 tatttctttt ggaatgccaa ttggtagttt agttgcagga acgcttattg atttgaatat
     1081 tgaattagtg ttaattgcta ttagcatacc ttattttttg ttttcttata ttttttatac
     1141 ggataatgga ttgaaagaat ttagtatata ttagaaatgt ttatgttcat tcaaaagcat
     1201 aatgactata actgaaaaag aaaagtgata tctttaaggt tgttcttctt ggtggtgaga
     1261 ttcgtgagac aacccaagct tttttcggaa agattaccaa tgctttgatg gataggatgt
     1321 actttagcaa gatgttttta gtggtaacgg tatcgtggat ggacgtgtaa taacctcttc
     1381 tttcgaggag tattttacta aaaaactagc cttggagcgt tccccagaaa cggacttact
     1441 cattgactct tcaaagattt ggggagaaga ttttgcttca tctgttcctt gaaaaaagtc
     1501 acagcagtca tcacagacga tagtactgaa caaaactatg aagagttaga aatttatacg
     1561 caggtgattg tataaaggat ctggaaatag ataagaagtt gattagtatt gacctaggtg
     1621 gtacaaatat taagattact gttctttcaa atgacggtga gattgaaact ttgtggagta
     1681 ttacaacaga tacaagtgag aaaggttctc aaattatatc ggacatcatc agttctatta
     1741 aaaataaatt gaccgaacgg aatattcctg atagcgacct tcttggaatc ggtatgggaa
     1801 gttgctcatc atactttcct tgtaaatcat aggggctata aactctccgt ctacttgtcc
     1861 tgcaacaatt gaagtctgct caaaacgccg tccgctaatc ttttcataga ctttctccct
     1921 tttaggagcc tagctttcta gtttgttctt tgatttttat tgagtatacc actattttac
     1981 tccctctggc aagggacttt gtctatgtgg agggattggg ctcctatgtg gtggagcttt
     2041 tctgttcttt ctgaaatatg gtataatagc actaatcaat ttctaggaaa atagatacag
     2101 aaaggggctg aaagatgtct catattattg aattgccaga gatgctggca aaccaaatcg
     2161 cggctggaga ggtcattgaa cgtcctgcca gtgtggtcaa agagttggta gaaaatgcca
     2221 ttgacgcggg ctctagtcag attatcattg agattgagga agctggtctc aagaaggttc
     2281 aaatcacgga taacggtcat ggaattgccc acgatgaggt ggagttggcc ctgcgtcgcc
     2341 atgcgaccag taagataaaa aatcaagcag atctctttcg gattcggacg cttggttttc
     2401 gtggtgaagc cttgccttct attgcgtctg ttagtgtctt gactctgtta acggcggtgg
     2461 atggtgctag tcatggaacc aagttagtcg cgcgtggggg tgaagttgag gaagtcatcc
     2521 cagcgactag tcctgtggga accaaggttt gtgtggagga tctctttttc aacacgcctg
     2581 cccgtctcaa gtatatgaag agccagcaag cggagttgtc tcatatcatt gatattgtca
     2641 accgtctggg cttggcccat cctgagattt cttttagctt gattagtgat ggcaaggaaa
     2701 tgacgcggac agcagggact ggtcaactgc gccaagcaat cgcaggaatt tacggtttgg
     2761 tcagtgccaa gaagatgatt gaaattgaga actctgacct agatttcgaa atttcaggtt
     2821 ttgtgtcctt gcctgagttg actcgggcta accgcaatta tatcagcctc ttcatcaatg
     2881 gccgttatat taagaacttc ctgctcaatc gtgctatttt ggatggtttt ggaagcaagc
     2941 ttatggttgg acgttttcca ctggctgtca ttcacatcca tatcgaccct tatctagcgg
     3001 atgtcaatgt gcatccaact aagcaagagg tgcggatttc caaggaaaaa gaactgatga
     3061 ctctggtttc agaagctatt gcaaatagtc tcaaggaaca aaccttgatt ccagatgcct
     3121 tggaaaatct tgccaaatcg accgtgcgca atcgtgagaa ggtggagcaa actattctcc
     3181 cactcaaaga aaatacgctc tactatgaga aaactgagcc gtcaagacct agtcaaactg
     3241 aagtagctga ttatcaggta gaattgactg atgaagggca ggatttgacc ctgtttgcca
     3301 aggaaacctt ggaccgattg accaagccag caaaactgca ttttgcagag agaaagcctg
     3361 ctaactacga ccagctagac catccagagt tagatcttgc tagcatcgat aaggcttatg
     3421 acaaactgga gcgagaagaa gcatccagct tcccagagtt ggagtttttc ggacaaatgc
     3481 acgggactta tctctttgcc caagggcgag atggacttta catcatagat cagcacgctg
     3541 ctcaggaacg ggtcaagtac gaggagtacc gtgaaagcat tggcaatgtt gaccagagcc
     3601 agcagcaact cctagtgccc tatatctttg aatttcctgc ggatgatgcc ctgcgtctca
     3661 aggaaagaat gcctctctta gaggaagtgg gcgtctttct agcagagtac ggagaaaatc
     3721 aatttattct acgtgaacat cctatttgga tggcagaaga agagattgaa tcaggcatct
     3781 atgagatgtg cgacatgctc cttttgacca aggaagtttc tatcaagaaa taccgagcag
     3841 agctggctat catgatgtct tgcaagcgat ctatcaaggc caatcatcgt attgatgatc
     3901 attcagctag acaactcctc tatcagcttt ctcaatgtga caatccctat aactgtcctc
     3961 acggacgtcc tgttttggtg cattttacca agtcggatat ggaaaagatg ttccgacgta
     4021 ttcaggaaaa tcacaccagt ctccgtgagt tggggaaata ttaaaagtat aaaaaagtct
     4081 gggaaaaatt ttcaaaatca gaaaaacgca taaaatcagg tgttcaaaaa ccttgatttt
     4141 atgcgtttta tcatagaaat atttacttca ttttttccta attcttttcg aaactctttt
     4201 taaacgacgt cagttttatc agtaatctca aaacagtgtt ttgagctaat tttgccagtt
     4261 ttgtctgtaa catcgaagtt gtgttttacc actctgcgac tggtttccta gtttgctcta
     4321 tgattttcac agagtattaa attgcgattt tgccaagttt ctttattcgt ctaaaagtag
     4381 agtctgttct atgcgtctaa tgtacgaatt aggttgacca tttcaatagc tccttgtgca
     4441 cactcagaac ccttatttcc tgctttagtg ccagctcgtt ctatggcttg ttcaattgta
     4501 tctgtcgtta gcacaccaaa cataacagga atttcgctat ttaaactgat ttgggcgatt
     4561 cccttagata cctcgctaca tacataatca taatgacttg tattccctct aatgacagct
     4621 cccaagcaga taattgcatc atatttttta ctttttgcca tttttgatgc aatcagtggt
     4681 atttcaaaag ctcctggaat ccaggctacc gctatatctt tctcgtttac attctctctt
     4741 ttgagattat ctagtgctcc agataataat tttgaagtta taaattcatt aaatctcgct
     4801 acaacaatac ctattttaat attgtttgct actaaattac cttcataagt gttcatttat
     4861 ttttcctcca tatttaaaat gtgacccatt cgatttttct ttgtttctaa ataaaaacta
     4921 tcgtaaggat tggcttctat ttcgattgat attctactgg aaatggtaat tccatatttt
     4981 tctaactgtt caaccttgtc aggattattt gtcagtaaat gaagtgactg aagtcccaga
     5041 tctttaagca tttgtgctcc aatatgatat tctcttaaat caccttcaaa gcctaatgca
     5101 agattggcat caagcgtatc catgccttga tcttgtaaat gataggcttt taatttattg
     5161 ataagtccaa ttcctcgtcc ctcctgtcgc aagtaaagta agacacccga accattctca
     5221 acaatcattt tcatagcttt atcgaattgc tgtccacaat cgcaacgtaa agagcctaaa
     5281 acatcccctg ttaaacattc ggagtggacc cgacataata cattggcttc atcctctatg
     5341 tttcccataa taagagcaag atgatgttcc ccatttagtt tatctatata gctaattgct
     5401 ttgaaattac cgtatctagt aggcatattg acagttgaaa ctcgttctac cagctgatca
     5461 tatacttttc tatattcttg taattctttg atggtaatta gtggaatgtt gtgtttcttc
     5521 gagaactgaa ttaaatcatc tgttctcatc attttgccat catgattcat tatttcacaa
     5581 cataggccac actcttttag tccagctaat tttaataaat caacagttgc ttctgtgtgt
     5641 ccatttcttt ctagaacacc accttttttt gcaattaaag gaaacatgtg tcctggcctg
     5701 cgaaaatcag agggtgttat atcttcagct acacacatac gtgcggtcag tcctctttcc
     5761 tcggcagaaa tacctgtggt cgtttcttta taatcaattg aaactgtaaa agcagtctta
     5821 tgattatctg tattgttttc aaccataggt gaaagcatta attgattagc taaactttcg
     5881 ctcataggca tacaaattaa tcctttggca taagtagcca taaaattaac attttctgtt
     5941 gtagctgctt gtgcagaaca aattaagtct ccttcatttt ctctatcctt gtcgtctata
     6001 acaagaacaa gtcgtccctt ctgcaatgct tctaatgctt cttgtatttt tcgatattcc
     6061 attgactgat tatcctttct gctaaaatcc attttgatat aatagttcct tagatatttc
     6121 tgattttgga gagttatcca tcagtttttg cacatattta cctaagatat cattttcaag
     6181 atttactgta ctcccgactt gtttactctt aagaatggtt tgttccaagg tatgagggat
     6241 aacagatact gaaaagttta ctttggagac tttagcgaca gtcagactaa tgccgtcaat
     6301 tgtaatagat cctttttcaa ctattaaatc taaaatttct ttttgtgtgt tgatttgata
     6361 ccatacagca ttatcatctt tttttattga cgagattttt cctgtaccat caatgtgtcc
     6421 tgtaacgacg tgacccccaa gtcgaccgtt gacagataag gctctttcta gattcacctc
     6481 acttccatgt tttaatagag taagagctgt tcgactccat gtttcattca ttacatcaac
     6541 tgtaaaggat tgatggttga aatgagtaac tgtaagacag ataccattta ctgctatact
     6601 atcgcctaaa tggatatccg ttaatatttt tgaggcttta attgatagtt tacaattacg
     6661 agagtctttc tgtattcttt caacttttcc gatttcttca attattcctg tgaacatgga
     6721 taaatcactt cactttctat gagatagtca tttcctattt gagaaaaagc ataaggtttc
     6781 aatctaatag cgtcatttgg caaagaaatg ccttcacctc cgacaggaaa cttggcactg
     6841 cctccaaaaa tttttggtgc aatatatatt ttcagctcat caacaatttg ttgttccaaa
     6901 gcactccaat tcattagact gcccccttct agaactaggc tatcaatctg catgtttcct
     6961 agatgttgca ttaaactcga taagtctata tgattgcctt ttttctttat ggaaagtatt
     7021 tcacagccat gattttgata tagcttcatt ttatttttgt cttcagagga agtggcaatg
     7081 taagttttaa tatcatttgc tgtttttacg attttagagg taagaggagt tcgtaaatgt
     7141 gtatcgcata tgatacggat aggatttttc ccttcctcca atctacatgt cagcaaagga
     7201 tcgtcttgaa taacagtatt gactcccacc ataattgcac taacatagtg tcgtaactga
     7261 tgcacatgct ttcttgcttc ttcttcagta atccatttgg attgatttgt tttagtggct
     7321 atttttccat ccattgacat tgcatatttc ataaaaacat agggtacatg ctgggtaata
     7381 tactttctaa aactttttat taagttaaga cactcatttt ctaaaattcc aacagtaact
     7441 tgaagattat tttcctcaag tatctttact ccttttccag atacaatagg attacagtct
     7501 aggcttccaa tgactactct tgtaatacca ctatcgatta tagcatctat acagggaggt
     7561 gttttcccga agtgacaaca gggttcaagt gttacataaa gcgtcgctcc gacaggggat
     7621 tctctacagt ttttaagagc atttctctca gcatgtgggc caccaaaaaa ctcatgataa
     7681 ccttgtccga tgatgtgatt atcttttaca ataactgcgc cgaccatagg attgggattg
     7741 acgtaaccag cccctttttg tgccagtttt attgctaatt tcatatattt tgaatcgctc
     7801 atctcactac ctccaaaaaa aatatacctt gaatagggga ctactcaagg catacaaaag
     7861 aaaacttatg cgattaacaa aaatactctg aaatgacaag taatcatttc agagcacgca
     7921 aaaagcacaa atatactttt atcttctttc atccagacta tactgtcggc tttggaattt
     7981 caccaaatca tgcctttcgg ctcgtgggct ataccaccgg tagggaatta caccctgccc
     8041 tgaagatagt tattcaatta cagatgatta tagtacttaa ttttgaatat gtcaacagat
     8101 aaataccgat tgtttttgat atactgtatt tgtgataatc gattctcgct cctcggataa
     8161 agaaaatatg atatactaga taaacgaaat aagagagaag gaatactatg tacgcatatt
     8221 taaaaggaat cattaccaaa attactgcca aatacattgt tcttgaaacc aatggtattg
     8281 gttatatcct gcatgtggcc aatccttatg cctattcagg tcaggttaat caggaggctc
     8341 agatttatgt gcatcaggtt gtgcgtgagg acgcccattt gctttatgga tttcgctcag
     8401 aggatgagaa aaagctcttt cttagtctga tttcggtctc tgggattggt cctgtatcag
     8461 ctcttgctat tatcgctgct gatgacaatg ctggcttggt tcaagccatt gaaaccaaga
     8521 acatcaccta cttgaccaag ttccctaaaa ttggcaagaa aacagcccag cagatggtgc
     8581 tggacttgga aggcaaggta gtagttgcag gagatggcct tcctgccaag gtcgcagtgc
     8641 aagcaagtgc tgaaaaccaa gaattggaag aagctatgga agccatgttg gctctgggct
     8701 acaaggcaac agagctcaag aaaatcaaga aattctttga aggaacgaca gatacagctg
     8761 agaactatat caagtcggcc cttaaaatgt tggtcaaata ggagcagaga atgacaaaac
     8821 gttgttcgtg ggtcaagatg accaacccgc tctacatcgc ctatcatgat gaggagtggg
     8881 gtcagcccct ccatgatgac caagcattgt ttgagttgtt gtgtatggaa acctatcagg
     8941 caggcctgtc ttgggaaacg gtactcaaca aacgccaagc tttccgagaa gcctttcata
     9001 gctatcaaat tcactcagtc gcagagatga ctgacactga attggaagcc atgctggaga
     9061 atccagctat cattcgaaat agagccaaga tttttgctac acgcgctaac gcccaagcct
     9121 ttctacagtt acaggcagag tacggctctt ttgatgccta tctttggtct tttgttgagg
     9181 ggaaaactgt cgttaacgat gttcctgatt atcgccaagc cccagctaaa acacccttat
     9241 ctgagaaatt agccaaagat ctcaaaaaac gaggcttcaa gttcacaggc ccagtcgccg
     9301 tattgtcttt tctacaggct gcagggctag ttgatgacca cgagaatgat tgtgagtgga
     9361 aaggtcttaa atgatgtcta acaaaaataa gggaattctg atttttgcga ttctctatac
     9421 agtcctcttt gtgtttgatg gcgttaaatt gctggcttct ttaatgccat ctgccattgc
     9481 aacttatctt gtttatgtag ttttagcttt atatggctcc ttcttgttca aggatagatt
     9541 gatccaacaa tggaaggaga ttagaaagac taaaagaaaa ttcttctttg gagtcttaac
     9601 aggatggctc tttctcattc tgatgactgt tgtctttgaa tttgtatcag agatgttgaa
     9661 gcagtttgtg ggactagatg gacaaggtct aaatcagtct aatattcaaa gtacctttca
     9721 agaacaacca ctactgatag ctgtttttgc ttgtgtcatt ggacctctgg tagaagaatt
     9781 atttttccgt caggtcttat tgcattactt gcaggaacgg ttgccaggtt tactaagcat
     9841 tattctggta ggacttgttt ttgctctgac tcatatgcac agtttggctc tatcagagtg
     9901 gattggtgca gttgattact taggtggagg ccttgccttt tctattattt atgtgaaaga
     9961 aaaagagaat atctactatc ccctacttgt tcacatgtta agcaacagcc tctccttaat
    10021 cattttagct atcagtatag taaaatgaaa taagaacagg acaaatcaat caggacagtc
    10081 aaatcgattt ctaacaatgt tttagaagta gaggtgtact attctagttt caatacactg
    10141 taacatgtga tgaaaatgcc agtaatgata ccgagaaaaa agctgagaaa cttttcccag
    10201 ctttatttgt tatagtcaaa gcgaatgact tgttcctgtg catctacatg agcatggacc
    10261 ccaaagggta caattgctct tggagttgcg tggccgacat tcagattata gacaatcggg
    10321 atattgttgt caatgatatc caatagtgcc tctttatagt cgtcatggaa agtttcatcc
    10381 ataggttttc cgaccaagag tccattgatg accgcgaata tgccagtgtc ctttaaagtt
    10441 agcaacatct ttttgaagtc ttctggctta ggctcttctt cgcttgtttc gagcaagagg
    10501 attttccctt cccagtctga caagtcaggg aaaagtttgt atttttggca gagttccgtg
    10561 ctatctgcgt atcgagagtt gtcaaagata tcgtagaggg attcgaggca accaccgagg
    10621 attttcccct cgaactgagc acttccttgc aacaagtcaa aacctgtatt tgtatgactg
    10681 acacgaggtg ttcccagggc cgtgggacta aaatcagttc gttcctcata ccaaacgtca
    10741 ctagggcgga tttctgaaat tcttcccgtc tcaatcaatt ctttaaagta gtgaaggcta
    10801 taggctagca tttctttgtc taattcacaa atgtctgcta aaaaggattg accataaaaa
    10861 gtcttgattc ctaatttatg caacatgagg tggttcatgg ttgtatccga gaagccaaga
    10921 aaaatttttt gtttgataac cttttggagt tggtcatttt caaaaagata aggtagcaag
    10981 cgataggtat cgtctccacc tatggcacat aggatcatgt cgatgctatc atcagaaaag
    11041 gcatgaatca aatcctctgc acgagcttca ggatggtcct tgataaagtc taatcctttt
    11101 aacgaatggg gcaaaaagat gggattgagt cccagaacct tgagacgttg gatacccaag
    11161 tccacttcgt gtttgacaaa atcctctccg ataatgccac tagataaact aacaatacca
    11221 atagtagaaa ccatatctca tcctcctaga aatagattga gcctatttta tcacaaaata
    11281 taagagaaag ctatgtggga tttcagaaga atacttttaa tactcttcga aaatctcttc
    11341 aaaccacgtc agttttatct gcaacctcaa agctgtgctt tgagcaacct gcagctagct
    11401 tcctagtttg ctctttgatt ttcattgagt ataactcaaa aaagtaagtg aaaaagcaac
    11461 cgcacctgca gttgctttta tcgttttctt attcttgata gcgtgttggt cctgcacttg
    11521 cactacggat attcaagcgt ttcttgagat agaagcgaag ggctaggaga gctgctccaa
    11581 taatagctag tggcaatgga tccagtactg ggttaaggct agctggtagg aagcttgttg
    11641 caaagaagac aagcaaccaa aggaacatag aagctaggat aactagtaca gatttccaga
    11701 aaggtggacg ttgactgcga tccatatctg gtccatagta ttggtaaaca aagtagtaca
    11761 tcaagtagaa ggcaaatcca ccaaccagtc caactaatag aagagtaatc aatccatagc
    11821 cgaaagcttg atctgccgca aagaaggttg tgagggcgct gacaagggca aagaggctag
    11881 tgatgaaaag agctgagtcc ataatcatca gttttgggtc atcattttct tttggatgct
    11941 ctttttcgta ctgctctttg acagtgaagc tatgagccca atgagttggt gcgccgtata
    12001 gggaacgggc agttgtacct ttagattgct cctcaaggat ttggggaatg acttcctcaa
    12061 aaatagtctg gatttcagcg tctgttttcc catctttgat gaattgttgg gtagcaatgt
    12121 ggacaaactc ttggtttttc ttagttaatt tttgtagatc aatttgagac ataggaactc
    12181 ctcttagaac cattttttat ggatgagata gagagtgagc gagacactca tagcaaaggc
    12241 gataaagacg attaaccaga aggcatttgg ctctccgttt aggggaattt cattatcctt
    12301 aaagttcatc ccgtaggcag aaaagaccat ggttgggatg gacatgacga tggtcacaag
    12361 ggccaaggtt ttcatgatgt tgttctggtt gttagaaatg atagaggcaa aggtctctgt
    12421 catagaatgc aagacgtttc cataaatatc tgccatctcg atggcctgtt gggtttcaat
    12481 cagggtgtct tcaagcaggt cttcgtcctc aaggtatttc ttgatattgc tggttgaact
    12541 ggtcaatttc ttaatcacgc gctcatttgt tttgagggag gccttgaaat agacgatagt
    12601 tttttccaat tccatgagct caatcaattc ttcattacga gttgattgat gcagttgact
    12661 ttcgatttgt tcactcttgc ggtcgattga acgaagggct gttaggtaaa gctctgcatt
    12721 gcgataaaga atttgaaaga taaaacgtga acgcatgaag gtatagaaat tacgcaatcg
    12781 acggttgata aagacatcaa ggacaggtag tggttccaaa cacgtagtga taatggtttc
    12841 ctcagtgatg ataataccaa gcgggatggt tacgtagtag gtgcggttat ttctttcctc
    12901 cgtgaccggc acgtctacga taatcagggt atactcgtct tcaatggtaa tacgagacat
    12961 ttcttccgca tcgagcggtg ctcgaaggtc ggcaatatca atatcgaagg tgttggcgat
    13021 ttcgagtgat tcattttgag tcggattgac gagattgatc caagtacccg gttcaagcgt
    13081 atcgatctct ttaaattcag ttgttgtaga gagaaaaact tgtttcatat cccttatcct
    13141 ttctcatttt tcagattttt tcacactgta ctattatact acaaaatcgg ccttttgggt
    13201 aatagaatct cacttttttt ggtataatgg taagcaataa tggactagaa agaacaaaga
    13261 tgcaagataa aattgtcatt catggggcgc gtgcccataa tttaaaaaat attgatgtgg
    13321 agattccgcg agacaagttg gttgtcgtga ctggcttgtc aggttcaggg aagtccagtc
    13381 tggcttttga tactctctat gcggagggac aacgtcgcta tgtagagagt ttgtctgcct
    13441 acgctcgtca gttcttggga aatatggaaa aaccagatgt agatgccatt gatggtctca
    13501 gcccagctat ttccatcgac cagaaaacga ctagtaaaaa tcctcgctcg acggtgggaa
    13561 cgacgactga aatcaatgac tatctgcgtc tcctctacgc acgtgtgggg acgccttact
    13621 gtatcaacgg acatggagct atcaatgctt cttctgtgga gcaaatcgtt gataaggttt
    13681 tggagttacc tgaacgccag cgcttgcaga tcttggctcc tgtcatccgc aagaaaaaag
    13741 gccaacataa gagtgttatc gagaaggttc agaaagacgg gtatgttcgt gttcgtgtgg
    13801 atggggaagt ctatgatgtg accgaagtgc cagagttgtc taagagcaag caacacaata
    13861 tcgatgtcgt ggttgaccgt attgttatca aggagggcat tcgtagccgt ctctttgatt
    13921 ccattgaagc tgcccttcgt atcgcagaag gttatgtgat tatcgacacc atggacgact
    13981 ctgagttgtt gttctctgag cattatgcct gtccagtttg tggatttact gtcccagagt
    14041 tagaacctcg tctcttctct ttcaatgctc cctttggctc ttgtagtgag tgtgacggct
    14101 tggggatcaa gctggaggta gatactgatt tggtagtgcc agatgccagc aaaaccttac
    14161 gtgagggagc gctagcacct tggaatccta tctcatccaa ctactatcca aacatgttag
    14221 agcaggccat gaaagtcttt ggagtggcta tggataagcc ttttgaggac ctgtcagaag
    14281 aagataagaa cttgattctc tatgggtcag atggtaagga attccatttc cactatgaga
    14341 atgaatttgg tggcgtgcgc gatatcgaca ttccgtttga gggagttatc aataatatca
    14401 aacgtcgtta ccatgaaacc aatagcgatt acactcgcac tcagatgcgt ctctacatga
    14461 atgagctgac ctgcggaacc tgtcaaggct atcgtctcaa tgaccaggcc ttgtctgtcc
    14521 gtgtgggcgg ccagcaaggg ccacatattg gagaaatctc agacctgtct atcgctgacc
    14581 acttggactt ggtgagccag ttaactttgt ctgaaaatga agccatcatt gctcgtccca
    14641 ttctcaagga aatcaaggat cgtttgacct tccttaataa tgtgggtctt aactatctaa
    14701 ccctgtcacg ttcagcagga accctttcag gtggggaaag tcagcgcatt cgtttggcaa
    14761 cccagattgg ttccaaccta tcaggcgtcc tctatatcct agacgagccg tcaatcggtc
    14821 ttcaccagag ggacaatgac cgcctgattg ccagtctgaa aaagatgcgt gacttgggca
    14881 atactcttat cgtggtggaa cacgacgaag ataccatgcg tgaagctgat tatctgattg
    14941 acgttggtcc tggtgccggt gtttttggtg gtgagattgt tgcagcaggt acacctaagc
    15001 aagtggctcg caacagcaag tctatcacag gtcagtactt gtcaggcaaa cgtgtcatcc
    15061 cagtaccaga agagcgccgt gtcggaaatg gtcgttttat tgaagtgaca ggagcgcgtg
    15121 agaacaactt gcaaaatgtc acggctcgct ttccactagg aaaatttatc gcagtgacag
    15181 gtgtgtcggg ttcagggaaa tcgactctaa tcaacagcat tctcaaaaaa gccattgccc
    15241 agaagctcaa ccgtaattca gacaaacctg gtaagtttaa gactattaca gggattgagc
    15301 atgtcgaccg tttgattgat attgaccaga gccctatcgg acggacgccg aggtctaacc
    15361 cagctaccta tacaggagtt tttgacgata tacgtgacct ttttgctcag acaaatgaag
    15421 ccaagattcg aggctacaag aaggggcgct tcagtttcaa cgtcaaggga ggtcgctgtg
    15481 aagcttgctc aggtgacggg attatcaaga ttgagatgca cttcttgcca gatgtttatg
    15541 tggcttgtga agtttgccac ggaacccgct ataacagtga aaccctagaa gttcactaca
    15601 aggaaaagaa tatttcgcag gtcttggata tgacggtcaa cgatgcggta gaatttttcc
    15661 aacacattcc gaaaattcaa cgcaaactcc agaccatcaa ggatgtaggg ctaggctatg
    15721 tgaccttggg acagccagct accacccttt ctgggggaga agcccaacgt atgaagttag
    15781 ctagcgaact ccacaaacgc tcgacaggaa aatctttcta cattctggat gagccgacga
    15841 cagggcttca tacagaggac attgctcgcc tgctcaaggt tttagctcgc tttgtagacg
    15901 atggcaatac agtcctcgtc atcgagcaca atctggatgt tatcaagacg gcagaccata
    15961 tcattgactt gggacctgag ggcggtgtcg gtggtggaac catcatcgta acaggaactc
    16021 cagaagaagt agctgccaac gaagccagct atacaggaca ctatttgaaa ggaaagttac
    16081 atcatgaata aacgcgtaca agcatttcta gctaaaatgc aagaaaaaga actagatggt
    16141 atcatcatca acaatcttaa aaacgtctat tatttgactg gtttttgggg ctcaaacgga
    16201 acagtcttta tcagtcgtga ccgtcaggtc ttagtgacag actctcgcta tatcatcgca
    16261 gctaagcaag aaaccagtgg ttttgagatt gtggctgatc gtgatgaatt ggctgtcatt
    16321 gcaggaattg ttaaggacat gggcttgact cgtatcggtt ttgaagatga gatttcagtg
    16381 tcttattacc accgtatgca ggcagctttt gcaggtttgg acttgtttcc gcaaactcag
    16441 tttgtggaag gtcttcgtat gattaaggat gaggcagaga ttgcagcgat tcgcaaggct
    16501 tgttctatct cagaccaagc tttccgcgat gcgcttgact ttatcaaacc aggaaaaact
    16561 gaaattgaga ttgccaactt ccttgacttc cgcatgcgtg agttgggagc atctggctta
    16621 tcttttgata cgatcctagc tagcggtatc aattcttcta aaccccatgc ccatccaatg
    16681 cacaaaccag tggagttggg agaagccatt accatggact tcggctgtct ctatgaccac
    16741 tatgtcagtg atatgacccg gactatctat ctagggcatg ttagcgatga gcaggcagag
    16801 atttacaata cggttctaaa agctaaccaa gccttgattg accaagctaa ggcaggctta
    16861 ggtttccgtg actttgacaa aatccctcgt gatattatca ttgaggcagg ttatggcgac
    16921 tactttactc acggcattgg ccacggtatt ggtctggata tccatgagga accctacttt
    16981 agccagactt ctacagaaac tattaagaca ggtatggcct tgaccgatga accaggtatc
    17041 tatatcgaag gcaaatatgg cgttcgtatc gaggatgata tcctgattac agagacaggt
    17101 tgtgaattat tgaccctagc tccaaaagag ttgatagtca tttagttgtt tgtcaagaaa
    17161 aaagtacgaa aatgacgaaa aaagtcaaaa aaaattaaaa ataggtcgca agtcggatgt
    17221 tttttatggt ataatagact aaactgatag taacatgtag cgaaaggggt aggtacatga
    17281 ttaaaattta tacagtctca agttgtacta gctgtaaaaa agcaaaaacc tggctcaatg
    17341 cccaccagtt aagttataaa gaacaaaacc ttggtaaaga aggaattacg agagaagaat
    17401 tactggatat tctaaccaaa acagataacg gaatagccag catcgtttcg tctaaaaatc
    17461 gctatgccaa agcccttgga gtggatattg aagatttgag tgtcaatgaa gttctcaatc
    17521 tgattatgga aacaccgaga attttaaaga gcccaatcct tgtagatgaa aaacgcctgc
    17581 aagttggcta caaggaagac gatattcgtg ccttcctacc acgctctgtc cgtaatgtag
    17641 aaaatgcaga agcacgttta cgtgcagctc tataaacatc aaggctggga gcaattccca
    17701 gtcttattct attttaattt caaaaagaaa gaagaaagaa atgaaaaaaa tagttcttgt
    17761 tagtctagct ttcctttttg tcctggttgg ttgcggacag aaaaaagaaa ctggaccagc
    17821 tacaaaaaca gaaaaggata cgcttcagtc ggcattgcca gttattgaaa atgccgagaa
    17881 gaatacagtt gtaaccaaga ctttggtctt gcccaagtca gatgatggta gccagcaaac
    17941 tcaaaccatt acatacaagg acaagacttt tttgagctta acgattcaac aaaaacgtcc
    18001 agtttctgat gagttgaaga cttatattga ccaacatgga gtggaagaaa ctcaaaaagc
    18061 tcttcttgaa gcggaggaga aggataagtc tatcattgaa gctcgtaaat tggtaggttt
    18121 caaacttgaa acaaaactat tgagcgcaac ggaacttcaa acaacgacta gttttgattt
    18181 tcaagttctg gatgtcaaga aggcttccca gttggaacat ctgaagaata ttggtttgga
    18241 aaatcttttg aaaaatgaac caagcaaata tatttcagac agattggcaa atggtgcgac
    18301 agaacagtag aaaatccaaa taaatagact gcctgaattg tagaaggtaa ggaattatgc
    18361 tataatggta ctattctaag gaaagagggt gttagaatgg gatttactga agaaacagta
    18421 cgttttaaat tggacgattc caataaaaaa gaaattagcg aaactttgac agatgtttat
    18481 gcttcgttga acgataaggg ttacaaccca attaaccaaa tcgtaggtta cgtattgagt
    18541 ggagaccctg cctacgttcc tcgttataat aatgcacgaa atcaaatccg taagtatgag
    18601 cgtgatgaaa tcgttgagga attggttcgc tactacctca aaggacaagg agtcgatcta
    18661 taacctatga gaattatggg attggacgtc ggttcaaaaa cggtaggggt ggcgattagc
    18721 gatccgcttg gttttacagc tcaagggctt gaaatcatcc agataaatga agaacaaggc
    18781 caatttggtt ttgaccgcgt taaggaattg gttgatactt acaaggtgga acgatttgta
    18841 gtgggcttgc ctaaaaacat gaacaataca agtggaccgc gcgtagaagc tagtcaagcc
    18901 tacggagcaa agctagaaga gttttttggt ttaccagtag actatcagga tgaacgcttg
    18961 acaacagtgg ctgctgagcg catgttgatt gaacaagcag atatcagtcg caataagcgc
    19021 aagaaagtca ttgataagtt agcagctcag ctgattttac aaaattattt agatagaaaa
    19081 ttttaatata aaggagaggc tatgtcacac gatcataacc acgaccacga agaacgtgaa
    19141 ctaatcacac tagtagatga gcaaggaaat gaaaccttgt ttgaaatcct tttgacaatt
    19201 gatggaaaag aagaatttgg taaaaactat gttcttctag taccagttaa cgcagaagaa
    19261 gacgaagacg gacaagttga aatccaagct tactcattca tcgaaaacga agatggaaca
    19321 gaaggcgaat tgcaaccaat cccagaagac tcagaagacg aatggaacat gattgaagaa
    19381 gtcttcaaca gctttatgga ggagtgatac agtctgggag actgtatcag cccaactccc
    19441 agaaatgggg aagagttgga acgtccagtg gatgttttta gcccagttcc tttaaataag
    19501 agagaactgg taggttcggg gggacgtttt tagcccagct cctttaaata agatagagct
    19561 ggtaagtctg ggagactgta tcagcccaac tcccagaaat ggggaagagt tggaacgtcc
    19621 agtgaacatt tttaccccaa ctcttagaaa ttgagaagag ttggaacatc tagtggatgt
    19681 ttttagccca tattcacatt catagtggct agttattagc taaccaggta agaggtcggg
    19741 acgaaaatcc tggccttgtt tttattagta accatacttt aatgcgataa ttgattggac
    19801 ttttgtcaag gagatgtgta tgtttgaagt agaagaatgg ctccatagtc ggattggttt
    19861 aaattttcga tcaggtttgg gccgaatgca gcaagcggtg gatttgttag gaaatcctga
    19921 gcagtcttac cctattatcc acgtaacagg gactaatggg aaaggatcta ccattgcttt
    19981 tatgagggaa ttatttatgg ggcatggcaa aaaagttgcg acctttacct cccctcatat
    20041 cgtctctatc aatgaccgaa tctgcattaa tgggcaacct atagcagacg cagactttat
    20101 ccgtttggct gatcaggtca aggagatgga gaaaacgctt ctgcaaactc ctgaccagtt
    20161 gtcctttttt gaattgctga ccttggttgc ttttctttat tttagggagc aagaggtgga
    20221 tttggtttta ttagaagtgg gaattggtgg cttacttgac acgaccaatg tggtaactgg
    20281 agagcttgct gtcatcacct ccattgggct tgaccatcaa gaaaccttgg gtgatagtct
    20341 agaagcaatt gcagagcaga aagctggtat tttcaaggct ggtaagaagg cagtgattgc
    20401 gaaattgcct ccagaagcta ggcttgtctg tcagaaaaaa gccgaatctt tagctgttaa
    20461 cctttatcag gcaggtcaag attttttaat gctgaatggt gatttttcaa gctctttact
    20521 aaatctttca cagctgaaca taggcttaga aggagtctat cagcaggaga atgcagcctt
    20581 ggcgttgcaa acttttcttc tttttatggg agaaggaaag gaagctgttg atgaacaggc
    20641 tgtaagaaag gccttggaac agacccattg ggctggtcgc ttggagcgta ttcgcccaca
    20701 gatttacttg gatggtgctc ataacctccc tgccttgact cgcttggttg agtttatcaa
    20761 agaaaaagag caggaaggct atcgacctca aatccttttt ggagccttga aacggaagga
    20821 ttatcaaggg atgttaggtt acctgactga aaaattgcct caggtggaac tcaaggtgac
    20881 cggctttgac tatcaggggg ctttggacga aagggatgtg acaggttacg atatagtttc
    20941 ttcttgccga gaatttatca gcgattttga agaaagagca gatgctcagg atttgctgtt
    21001 cgttacaggg tctctctatt ttatctcaga agtacggggc tacctgctgg accgtgagca
    21061 gataaattga cccccttgtt tgaacttgtt atactagggg aattgctggt tagagaaaat
    21121 ttctctaaat tggtagcaga aaggaaattc atcatgaaat taaaaagatt cacactttct
    21181 cttgcttctc tagcaagttt tagtctctta gtagcttgtt cacaaagagc tcaacaggtt
    21241 caacagcctg ttgctcagca gcaggtccaa cagcctgctc aacaacctgc tcaacagaat
    21301 accaatactg caaatgcagg aggtaaccaa aatcaagcgg ctccagtaca aaaccaacct
    21361 gttgctcaac cgaccgatat tgatgggact tatactggtc aggatgacgg agaccgtatc
    21421 actttagtgg taactggaac gactggtaca tggactgagc tcgaatctga cggggatcag
    21481 aaagtcaaac aggttacatt tgattcagca aatcaacgca tgattattgg cgatgatgtc
    21541 aaaatttaca ctgtaaacgg taatcaaatc gtcgtagatg atatggatag agacccatcg
    21601 gaccaaatcg ttttaactaa ataagactat gcaagtagaa agggctggat ttttcagcct
    21661 ttttactttt acctagaaaa taatcataaa tacttgcctt ctaccgaata cctgcgttat
    21721 actagtatca attacttgtt tttagcattc aaactatcaa ggaggggata tgaaatatag
    21781 gaaatttcaa ttattgatgt ccaagtatgg ctttagtctt tcgattatgt tacttgaact
    21841 ttgtcttgtt tttggtctct ttctttattt aggacgcatg gctcccattt tatggattac
    21901 tgtcctcatt ctactgagta tcatcacaat catttcgata gtcaaccgta atacgactcc
    21961 tgagaataag gtaacctggt tgttagtagc ctttgtgcca gtatttggtc ccttgctcta
    22021 tctgatgttt ggtgaaaggc gattgtccaa aaaagaaatc aaacaactga agaagctagg
    22081 ctctatgcat ttccaagaag caaatagcca gctactaaaa gagaaattaa aagaaagtga
    22141 caaggcagct tatggagtca tcaagtcctt attgagtatg gataccaatg ctgacatcta
    22201 tgatcaaact gcctctacat tttttcctaa cggagaagct atgtggaaaa agatggtaga
    22261 agatcttaaa aaggctgaga aatttatttt cttggaatat tacattatag aagaaggttt
    22321 gatgtggaat cgcatactag atatactaga gcaaaaggta gctcagggtg tagaggttaa
    22381 gatgctctat gatgatatcg gctgtatggc tactttaaca ggagattatg cacatcgact
    22441 tcgtcagctg ggcatcgagg cccataaatt caataaagtt attcctcgtt tgacagtggc
    22501 ttataataac agagatcata gaaaaatatt gattgttgat ggtcagatag cctatactgg
    22561 tgggatcaat ctggcagatg agtacattaa ccacgtcgag agatttggtt attggaagga
    22621 tagtggaatt cgcttagacg gactagcagt aaaagctctg acacgcttat ttttgaccac
    22681 ttggtacatt aatcgaggag aaattagtga ttttgatcaa tatcatttag aaaatcattc
    22741 tatcccgagt gacggtttaa ccattccata cggaagtgga cccaagccaa tttttcgagc
    22801 gcaggtaggg aaaaaagttt atcagagttt aatcaatcaa gcaacagaat cggtctatat
    22861 tacgacacct tatttgatta tagattatga tttaacagag acaatcaaaa atgcagctat
    22921 gagaggggtc gatgttcgaa ttatcacccc ttacatacca gataagaagt tcattcagtt
    22981 agtcacgaga ggagcttatc ccgaccttct ttctgctggt gttcggattt atgagtatag
    23041 tccaggtttt attcatagta agcagatgtt ggtagacgaa gattttgcgg tggtggggac
    23101 aatcaatctc gactaccgga gcttggtaca ccattatgaa aatgcagtct tactctataa
    23161 aactccttct ataagggaaa tcgcccgaga ttttcgaaat atatttgcag attctcagga
    23221 agtctatcct cattctatca aaacgagctg gtatcaaaag cttgtaaaag aaatcgccca
    23281 gctattcgcc cctatcttat aagaaatcta gaacttagga tacaaaccag acctattttt
    23341 cttgtctttt acgaatagaa agaaagagga ggggaattca tgctaactaa ggaagaagtg
    23401 aatgatatga ttgagttcaa acagacacat ttacaccgct ttcgggaaat tgaaaccttt
    23461 gtgaaactat gcaaaaaatc actcaaacag ccatcgcaag ctgacaatcc taggactttt
    23521 tgatatacta agacagataa attgaacagg agaaacgata tgagtgtgta tggtagagta
    23581 gaagaagttc ataaggagaa tcgtgaacct ctagaatacc aaatcgaaca agaatcgcat
    23641 catcgtgaat caagtcgtct tcctttggtg aaaattttac tatggagtac gcttgtaacg
    23701 gggataactc taggagtacc gctattactc gacttaatga gtgcacaaga agtgcaggat
    23761 ttttatgcag gttgggcgct tcatcagaca gggaagattt acagcgacta ttatggaagt
    23821 caaggtttgc tttattattt gctgacttac gtgagtcagg gcggattttt ctttgccatc
    23881 tttgagtggt tagccttggt agcaggagga tttttccttt ttagatcagc ggacaccttg
    23941 acagagcaag gagaccaagc tggacatctg gtgactattt tttacatgct agttacaggt
    24001 cttgcttttg gtggaggcta tgcgactctt ttagcgcttc ctttcttatt cgcagccttt
    24061 agtttagttg cggcttacct aagcaatcca agccatgata agggatttgt acggattggg
    24121 ctagctttgg caggcggatt tttctttgct cccttatcat cgctcctgtt tattgctgta
    24181 gtgagtttag gcttgttggt ctttaacctt gggcatagac gctttgcgca tgggttttat
    24241 cagtttcttg cagtggcttt aggtttttca cttgtctttt atccaactgc ctactatagt
    24301 gctgcaacag gaagttttgg ggatgcgatt agtggtattc gttatcctat tgacagtatt
    24361 cgctttgatt ttacttctaa aattttagag aatatgtttt tttatggctt gttgtccctt
    24421 ggtttgggat ttgtggtttg tatcttttta ggtctctttc aatccaaacc ctttaaacta
    24481 tacgtcattt cggttcctgc aagtcttgtg gtaattttag gtttgatttt acttttcttt
    24541 tcacaagagc ctctgcacgc ttcttatttg atggtcgtct tccctgtttt cctactttta
    24601 ttggtaacca atattaagag tcaacagagg gggcgtagtg ctagaagaag ccgaagagaa
    24661 acgccagtta gcctatggag tcgtttcttc aaaggaaatc tatatctgct agtttttgag
    24721 tttgtctatc ttttgtctgt tccttttttg atgaagtttg tcctttatcc agtaccttat
    24781 caagaacgta atcgtcttgc tgatttggta aaagaggaga caaatacgga agatgctatc
    24841 tatgcatggg atgatactgc gactctttat cgtaagagtg agcgcttgtc gccatcggcg
    24901 attttgtccc cgttgcacta tacagcaact gaggaaaatc gtaataagtt acttaatgac
    24961 ttgaaagaaa aacaacctaa ggtgattgtg gtaaatgata aggtggtagt ctggtctgaa
    25021 gtggaaacac tcttaaaaga aaattaccaa caagtaaaga ctgattactc agagtttaaa
    25081 gtctataaaa ttaaataacc aaatcaatat cttgtgtatt tttaaaaatt ttaggatttt
    25141 taacacaaga tattgatttt tctttttaga gtggtataat actttttaga aagaacattt
    25201 tagaaaagag catgcatatg attgcactag aagaaaaaat tacaattttg ccaactctct
    25261 tcgtcgagaa acgagatggg agacgtgttg tatttgatgt ggacaagatt gacaaggctc
    25321 tccacaaggc ggctgacaag gttatggatg tgacacccct ggttgaaaaa cgcctcaatg
    25381 atctgactga gcgaattatt acagaaattc atagtcgctt tccacaggga attaagattt
    25441 acgaaattca aaatatcgta gaacatgaac tccttgaagc caaagaatat gcgctggctg
    25501 aggagtatat tacttatcgg acacagaggg attttgagcg ctcaaaagcg acggatatca
    25561 actttagtat tcataaactt ctcaacaaag accagacagt tgtcaatgaa aacgctaata
    25621 aagacagtga tgtctttaac actcagcgtg atttgacagc agggattgtt gggaaatcaa
    25681 tcggactgca aatgcttcct aagcacgtag ccaatgccca ccaaaagggg gatatccact
    25741 atcacgattt ggactacagt ccctataccc ctatgaccaa ctgctgtttg attgatttta
    25801 agggtatgtt ggaaaatggt tttaagattg gaaatgcaga ggtagagagt cccaagtcta
    25861 tccagactgc gacagcacag atttctcaaa tcattgccaa cgttgcttct agccagtacg
    25921 gtggctgttc agctgaccgt atcgatgaaa ttttggcgcc ttatgcagag aagaattatc
    25981 aaaaacatct caaagatgca gaagagtggg tattgcctga aaaacgggaa gattacgctt
    26041 ggaagaaaac gcaaaaggac atctacgatg ccatgcaatc tcttgagtat gaaatcaata
    26101 ctctcttcac ttcaaatgga caaacacctt ttacttcgtt aggttttggt ctgggaacca
    26161 gtcgttttga acgagaaatt caaaaagcta ttttaaacat tcgcatcaag ggtcttggtt
    26221 cagaacaccg tacggctatc tttcctaaac ttattttcac tctgaaaaga ggccttaatt
    26281 tagaggaagg aactcccaac tacgacatca agcagttggc tcttgagtgt gcaaccaaac
    26341 ggatgtatcc agatgtttta tcttacgata agattgttga cttgacagga tccttcaagg
    26401 tgcctatggg ttgccgttct ttcctccaag gatggaagga tgaaaatggt gtagaagtca
    26461 attcaggtcg catgaatctg ggtgttgtga cggttaatct gcctcgtatt gctcttgagt
    26521 ctgaaggtga tatgaataag ttctgggaaa tcttcaacga gcgaatgaat atcgcagaag
    26581 atgctcttgt ttaccgtgtc gaacgcacta aagaggcgac accagcaaat gctcctattc
    26641 tttatcagta cggtgctttt ggccatcgtc taggtaaaga agaaagtgtt gaccagctct
    26701 ttaagaatcg tcgtgctacc gtttcgctgg gctatatcgg cttgtatgaa gtagcgacag
    26761 ttttctttgg taacagctgg gaaagtaatc cagatgctaa ggaattcacg ctagacatca
    26821 ttcacgatat gaaacgccgt gtagaagagt ggtcagacca atatggctac catttctcta
    26881 tctactcaac accatccgaa agtctgacag accgtttctg ccgactagat atagacaagt
    26941 ttggctctat tcctgatatc acagacaagg aatactacac caactctttc cactacgatg
    27001 ttcgtaaaaa tccaacaccg tttgaaaaat tggactttga gaaagtctat ccagaagcag
    27061 gtgcgtcagg tggtttcatc cattattgtg agtatccagt ccttcagcaa aatccaaagg
    27121 ccttggaagc tgtctgggat tatgcttatg accgtgtagg ctatctaggc accaatactc
    27181 cgattgaccg ttgctacaag tgtgactttg aaggggattt tgaaccaact gagagagggt
    27241 ttgcttgtcc aaactgtggc aatagcgacc ctaaaacagt agatgtggtg aagcgaactt
    27301 gtggctacct aggtaatcct caagcaagac cgatggtcaa cgggcgtcac aaggaaatcg
    27361 ctgcgcgtgt caaacatatg aatggttcaa cgattaaaat agctgggcat caagtaacaa
    27421 attagaaaga aatgaaatgg gaaaatatca actagacgat aaggggcgcg cacaagtgac
    27481 ccgttatcac gagaaacact ctaaaggtgg agctggtaag aaagaacgct tgcttagctt
    27541 cagagaacaa tttttaaaca agaacaagaa aaaataaaag tgagagccag ctctcgcttt
    27601 tctcatagtg ggaggtaagg atggaattac gcagaccaag attagcggat aagaaagctg
    27661 ttttagatat gatgacagag tttgaaaaga gtcaatcagc ccatgatgga ggattttggg
    27721 atgttgagga tttttcttac gaggagtggc tagaaggtaa ccgtaataaa gaaatgggaa
    27781 tatggctacc tgaaaatcgc gttccatcga tacagttcgt attgtttggt gaatcaggtt
    27841 gtgctctagg atttttgaat ctgcgattga gactgaatga ggggttactg aattatgctg
    27901 gccacattgg ctactccatc cgtccatctg aaagaggcaa gggttatgca aaagagactc
    27961 tccgtcaggg cttgcaagtt gctaaggaaa agaacatcaa gaaagctctg gtgacctgta
    28021 gcgtgaataa tcttgctagc agagcagtca ttctagcaaa tggtggaata tttgaggatg
    28081 ctcgcaatgg agtcgagcgt tattggatag aggtagcgaa tgaataatcc aaaaccacaa
    28141 gaatggaaaa gcgaggaact tagtcaaggt cgtatcattg actacaaggc ctttaacttt
    28201 gtggacggcg aaggcgtgcg taactctctc tatgtatcag gctgtatgtt tcactgcgag
    28261 ggatgttata atgttgcgac ttggtctttt aatgctggca ttccctatac agcagaatta
    28321 gaagagcaga tcatggcaga tcttgctcag ccctatgttc aaggtttgac tttgttggga
    28381 ggggagcctt ttcttaatac tgggattctc ttgccacttg ttaagcggat tcggaaggaa
    28441 ttgccagaca aggacatctg gtcctggacc ggctacactt gggaagaaat gatgttggaa
    28501 actccagata aactggaatt cttgtcactg attgacattc ttgtcgatgg aagatatgat
    28561 cgaactaaga gaaatcttat gctccagttt cgaggttcgt ctaaccaacg aattatcgat
    28621 gtgcaaaaat cgctcaaaag tgggcaagta gtgatttggg acaagctcaa tgacggaaaa
    28681 gaaagctatg aacaggtgaa gagagaatga agaaaaaaga cttagtagac caactagtct
    28741 cagagatcga gacggggaaa gtcaggacac tgggaatata cggtcatgga gcttcaggta
    28801 aatcaacctt tgcacaggaa ttgtaccaag ctttagattc tactacagta aatttgctag
    28861 agacagatcc ttatatcacc tcaggacgcc atctggtagt acccaaggac gcgccgaatc
    28921 aaaaggtgac agccagtctg ccagtggcgc atgaactgga gagtttgcag agagatatcc
    28981 ttgccttgca ggcgagtatg gatgtcttga caattgaaga accttggaag gctagtgagg
    29041 tcttgtctgg agccaaacca attttgattg tcgaagggat gtctgttggc tttctaccca
    29101 aggaactctt tgaaaaaacc atctgtttct acacggatga ggagaccgaa ttaaagcgac
    29161 gccttgctag agatacgact gtgagaaatc gcgatgcatc ctttatattg gctagccatc
    29221 agatgagacg ggagcagtat ctgcgatact ataaagaaac tgagtctaag gcggatatct
    29281 tagtggacca atcagaagat aaatttgatg tcaagaggac ttaacttatg tatagaagaa
    29341 atatagaaga aaatccctaa ttttagaaag aaaaagtagg aaaacagttt catccccaaa
    29401 aaacgaaaaa acctaacaaa tcccttgcaa tcgcagggga tttgtgttat tctattatgg
    29461 tgctgtaaat tacagcctta gttttgatgc aagaggttgc gacacgctcg gttgcattgc
    29521 cacgcaacac cgcgtcggtt ttcttgtgga gctagcctat tatcttaaat agacgaaaag
    29581 gagaaaaaga tggcaaacaa aaaaatccgt atccgtttga aagcttacga acaccgtacg
    29641 cttgacacag cggctgcaaa aatcgtagaa tcagctactc gtacaggtgc acaagttgcg
    29701 ggtccaatcc cacttccaac tgaacgtagc ctctacacaa tcattcgtgc gactcacaaa
    29761 tacaaagact ctcgcgaaca atttgaaatg cgtacacaca aacgtttgat cgatatcgtt
    29821 aacccaactc aaaaaacagt tgatgccttg atgaaattgg atcttccaag tggtgtaaac
    29881 gtagaaatca aactttaatc taaaatataa aagagcagag gctggtgttt caatctaatt
    29941 gaacacgggc taaactcggt gtgaaaaaga taaacttcct cgcgtctgct agacactgtg
    30001 tcagtttcct attttcactt tgagtttgac gccctttgta tcttagactt gagcataaaa
    30061 aacgctcgtt aaaaactttt tgaataaaaa atatagaaaa ggaactattt tctcatgaca
    30121 aaaggaatct tagggaaaaa agtgggaatg actcaaatct tcactgaagc tggcgaattg
    30181 atccctgtaa cagttattga agcaactcca aacgttgttc ttcaagttaa aactgttgaa
    30241 acagacggat acaacgctat ccaagttggt ttcgatgaca aacgcgaagt attgagcaac
    30301 aaacctgcta aaggacatgt agcgaaagct aacacggctc ctaagcgctt cattcgtgaa
    30361 ttcaaaaacg ttgaaggctt ggaagttggt gctgaaatta cagttgaaac attcgcagct
    30421 ggagacgttg ttgacgtaac tggtacttct aaaggtaaag gtttccaagg tgttatcaaa
    30481 cgccacggac aatcacgtgg accaatggct cacggttctc gttaccaccg tcgtccaggt
    30541 tctatgggac ctgttgcacc taaccgcgta ttcaaaggta aaaaccttgc aggacgtatg
    30601 ggtggtgacc gcgtaacaat tcaaaatctt gaagttgtac aagttgttcc agaaaagaac
    30661 gttatcctta tcaaaggtaa cgtaccaggt gctaagaaat ctcttatcac tatcaaatca
    30721 gcagttaaag ctggtaaata ataaagaaag gggaaatcag tcacaatggc aaacgtaaca
    30781 ttatttgacc aaactggtaa agaagctggc caagttgttc ttagcgatgc agtatttggt
    30841 atcgaaccaa atgaatcagt tgtgtttgat gtaatcatca gccaacgcgc aagccttcgt
    30901 caaggaacac acgctgttaa aaaccgctct gcagtatcag gtggtggacg caaaccatgg
    30961 cgtcaaaaag gaactggacg tgctcgtcaa ggttctatcc gctcaccaca atggcgtggt
    31021 ggtggtgttg tcttcggacc aactccacgt tcatacggct acaaacttcc acaaaaagtt
    31081 cgtcgcctag ctcttaaatc agtttactct gaaaaagttg ctgaaaacaa attcgtagct
    31141 gtagacgctc tttcatttac agctccaaaa actgctgaat ttgcaaaagt tcttgcagca
    31201 ttgagcatcg attctaaagt tcttgttatc cttgaagaag gaaatgaatt cgcagctctt
    31261 tcagctcgta accttccaaa cgtgaaagtt gcaactgcta caactgcaag tgttcttgac
    31321 atcgcaaata gcgacaaact tcttgtcaca caagcagcta tctctaaaat cgaggaggtt
    31381 cttgcataat gaatttgtat gatgttatca aaaaacctgt catcactgaa agctcaatgg
    31441 ctcaacttga agcagggaaa tatgtatttg aagttgacac tcgtgcacac aaacttttga
    31501 tcaagcaagc tgttgaagct gctttcgaag gtgttaaagt tgccaatgtt aacacaatca
    31561 acgtaaaacc aaaagctaaa cgtgttggac gttacactgg ttttactaac aaaactaaaa
    31621 aagctatcat cacacttaca gctgattcta aagcaatcga gttgtttgct gctgaagctg
    31681 aataatctaa ggaggaaata tcgtgggaat tcgtgtttat aaaccaacaa caaacggtcg
    31741 ccgtaatatg acttctttgg atttcgctga aatcacaaca agcactcctg aaaaatcatt
    31801 gcttgttgca ttgaagagca aggctggtcg taacaacaac ggtcgtatca cagttcgtca
    31861 ccaaggtggt ggacacaaac gtttctaccg tttggttgac ttcaaacgta ataaagacaa
    31921 cgttgaagca gttgttaaaa caatcgagta cgatccaaac cgttctgcaa acatcgctct
    31981 tgtacactac actgacggtg tgaaagcata catcatcgct ccaaaaggtc ttgaagtagg
    32041 tcaacgtatc gtttcaggtc cagaagcaga tatcaaagtc ggaaacgctc ttccacttgc
    32101 taacatccca gttggtactt tgattcacaa catcgagttg aaaccaggtc gtggtggtga
    32161 attggtacgt gctgctggtg catctgctca agtattgggt tctgaaggta aatacgttct
    32221 tgttcgtctt caatcaggtg aagttcgtat gattcttgga acttgccgtg ctacagttgg
    32281 tgttgtcgga aacgaacaac atggacttgt aaaccttggt aaagcaggac gtagccgttg
    32341 gaaaggtatc cgcccaacag ttcgtggttc tgtaatgaac cctaacgatc acccacacgg
    32401 tggtggtgaa ggtaaggcac cagttggtcg taaagcacca tctactccat ggggcaaacc
    32461 tgctcttggt cttaaaactc gtaacaagaa agcgaaatct gacaaactta tcgttcgtcg
    32521 tcgcaacgag aaataatatt aaactagtcg cttaagcaac tagtaaatcc gccagctcgg
    32581 tagcgctcca taggagtgca agccgctgtg gtacaacatt taaaggagaa aatataaaaa
    32641 tgggacgcag tcttaaaaaa ggacctttcg tcgatgagca tttgatgaaa aaagttgaag
    32701 ctcaagctaa cgacgaaaag aaaaaagtta ttaaaacttg gtcacgtcga tcaacgatct
    32761 tcccaagttt cattggttac actattgcag tttatgacgg acgtaaacac gtacctgttt
    32821 acatccaaga agacatggta ggccacaaac ttggtgaatt tgcaccaact cgtacttaca
    32881 aaggtcacgc tgcagacgac aagaaaacac gtagaaaata aggagaacat aaatggcaga
    32941 aattacttca gctaaagcaa tggctcgtac agtacgtgtt tcacctcgta aatcacgtct
    33001 tgttcttgat aacatccgtg gtaaaagcgt agccgatgca atcgcaatct tgacattcac
    33061 tccaaacaaa gctgctgaaa tcatcttgaa agttttgaac tcagctgtag ctaacgctga
    33121 aaacaacttt ggtttggata aagctaactt ggtagtatct gaagcattcg caaacgaagg
    33181 accaactatg aaacgtttcc gtccacgtgc gaaaggttca gcttcaccaa tcaacaaacg
    33241 tacagctcac atcactgtag ctgttgcaga aaaataagga ggtaaaatcg tgggtcaaaa
    33301 agtacatcca attggtatgc gtgtcggcat catccgtgat tgggatgcca aatggtatgc
    33361 tgaaaaagaa tacgcggatt accttcatga agatcttgca atccgtaaat tcgttcaaaa
    33421 agaacttgct gacgcagcag tttcaactat tgaaatcgaa cgcgcagtaa acaaagttaa
    33481 cgtttcactt cacactgcta aaccaggtat ggttatcggt aaaggtggtg ctaacgttga
    33541 tgcactccgt gcaaaactta acaaattgac tggaaaacaa gtacacatca acatcatcga
    33601 aatcaaacaa cctgatttgg atgctcacct tgtaggtgaa ggaattgctc gtcaattgga
    33661 gcaacgtgtt gctttccgtc gtgcacaaaa acaagcaatc caacgtgcaa tgcgtgctgg
    33721 agctaaagga atcaaaactc aagtatcagg tcgtttgaac ggtgcagata tcgcccgtgc
    33781 tgaaggatac tctgaaggaa ctgttccgct tcacacactt cgtgcagata tcgattacgc
    33841 ttgggaagaa gcagatacta catatggtaa acttggtgtt aaagtatgga tctaccgtgg
    33901 tgaagttctt ccagctcgta aaaacactaa aggaggtaaa taaccaatgt tagtacctaa
    33961 acgtgttaaa caccgtcgtg aattccgtgg aaaaatgcgc ggtgaagcaa aaggtggaaa
    34021 agaagtagca ttcggtgaat acggtcttca agctacaact agccactgga tcactaaccg
    34081 ccaaatcgaa gctgctcgta tcgccatgac tcgttacatg aaacgtggtg gtaaagtttg
    34141 gattaaaatc ttcccacaca aatcatacac tgctaaagct atcggtgtgc gtatgggatc
    34201 tggtaaaggg gcacctgaag gttgggtagc accagttaaa cgtggtaaag tgatgttcga
    34261 aatcgctggt gtatctgaag agattgcacg tgaagcgctt cgacttgcta gccacaaatt
    34321 gccagttaaa tgtaaattcg taaaacgtga agcagaataa ggagaaggca tgaaacttaa
    34381 tgaagtaaaa gaatttgtta aagaacttcg tggtctttct caagaagaac tcgcgaagcg
    34441 cgaaaacgaa ttgaaaaaag aattgtttga acttcgtttc caagctgcta ctggtcaatt
    34501 ggaacaaaca gctcgcttga aagaagttaa aaaacaaatc gctcgtatca aaacagttca
    34561 atctgaagcg aaataataga ctagggaagg agaaatttca atggaacgca ataatcgtaa
    34621 agttcttgtt ggacgtgttg tatctgacaa aatggacaag acaatcacag ttgtagttga
    34681 aacaaaacgt aaccacccag tctatggtaa acgtattaac tactctaaaa aatacaaagc
    34741 tcatgatgaa aacaatgttg ccaaagaagg cgatatcgta cgtatcatgg aaactcgccc
    34801 gctttcagct acaaaacgtt tccgtcttgt agaagttgtt gaagaagcgg tcatcatcta
    34861 atcaaacctg aaaggagaaa actgaaatga ttcaaacaga aactcgtttg aaagtcgcag
    34921 acaacagcgg tgctcgcgaa atcttgacta tcaaagttct tggtggttca ggacgtaaat
    34981 ttgcaaacat cggtgatgtt atcgtggcat ctgtaaaaca agctactcct ggtggtgcgg
    35041 ttaaaaaagg tgacgttgtt aaagcagtta tcgttcgtac taaatcaggt gctcgtcgtg
    35101 ctgatggttc atacatcaaa tttgacgaaa acgcagcagt tatcatccgt gaagacaaaa
    35161 ctcctcgcgg aacacgtatc tttggcccag ttgcacgtga attgcgtgaa ggtggcttca
    35221 tgaagatcgt gtcacttgct ccagaagtac tttaattttt aggaacaaac tagtccccta
    35281 gcttcaagct agggtgccct tatgggcgta agaaaaatca aggagaaacc taatgtttgt
    35341 aaaaaaaggc gacaaagttc gcgtaatcgc tggtaaagat aagggaacag aagctgttgt
    35401 ccttactgcc cttccaaaag taaacaaagt tatcgttgaa ggtgttaaca ttgttaagaa
    35461 acaccaacgt ccaactaacg agcttcctca aggtggtatc atcgagaaag aagcagctat
    35521 ccacgtatca aatgttcaag ttttggataa aaatggtgta gctggtcgtg ttggttacaa
    35581 atttgtagac ggtaaaaaag ttcgctacaa caaaaaatca ggcgaagtgc ttgattaatc
    35641 acgaaggaaa ggagaagtat aatggcaaat cgtttaaaag aaaaatatct taatgaagta
    35701 gttcctgctt tgacagaaca attcaactac tcatcagtga tggctgtgcc taaagtagat
    35761 aagattgttt tgaacatggg tgttggtgaa gctgtatcaa acgctaaaag ccttgaaaaa
    35821 gctgctgaag aattggcact tatctcaggt caaaaaccac ttatcactaa agctaaaaaa
    35881 tcaatcgccg gcttccgtct tcgtgaaggt gttgcgatcg gtgcaaaagt tacccttcgt
    35941 ggtgaacgta tgtacgaatt cttggataaa ttggtatcag tttcacttcc acgtgtacgt
    36001 gacttccacg gtgtcccaac aaaatcattt gatggacgcg ggaactacac acttggtgtg
    36061 aaagaacaat taatcttccc agaaatcaac ttcgatgacg ttgacaaaac tcgtggtctt
    36121 gacatcgtta tcgtaacaac tgctaacact gacgaagagt cacgtgcatt gcttacaggc
    36181 cttggaatgc cttttgcaaa ataatatagg aggtaaatct aatggctaaa aaatcaatgg
    36241 tagctagaga ggctaaacgc caaaaaattg ttgaccgtta tgctgaaaaa cgtgctgcat
    36301 taaaggcggc aggggactac gaaggtttat ctaaattacc tcgcaacgcc tcaccgactc
    36361 gtttacataa tcgttgtagg gttacggggc gcccacattc agtttaccgc aaatttggtc
    36421 tgagtcgtat cgcttttcgc gaacttgcgc ataaaggtca aattcctggt gtaacaaaag
    36481 catcttggta atttaagata tcaagagtgt caaaactcca agtaaaaata ggaaacttga
    36541 cgaagaaact aaagtttcta ggaaagttta tctttttcac acagagttta gcccgggttc
    36601 aattgggctt gccaatttga acacgagcta cagctttggc aaaaaagacc aatttgcttt
    36661 ggagcattgc ttctgcatta aattgtctat ttttgctcgt gctgttacgc tctttgtatc
    36721 atgtattaac tagcaagtgc aacttgcaaa ctactagtaa gaggagaaaa acaaaatggt
    36781 tatgactgac ccaatcgcag acttcctaac tcgtattcgt aatgctaacc aagctaaaca
    36841 cgaagtactt gaagtacctg catcaaacat caaaaaaggg attgctgaaa tccttaaacg
    36901 cgaaggtttt gtaaaaaacg ttgaaatcat tgaagatgac aaacaaggcg tcatccgtgt
    36961 atttcttaaa tacggaccaa atggtgagaa agttatcact aacttgaaac gtgtttctaa
    37021 accaggactt cgtgtctaca aaaaacgtga agaccttcca aaagttctta acggacttgg
    37081 aattgccatc ctttcaactt ctgaaggttt gcttactgat aaagaagcac gccaaaagaa
    37141 tgttggtggt gaggttatcg cttacgtttg gtaaaatcaa gatacaaagc tcgtaaagaa
    37201 caaagcaaaa ttaggaagtt ggagaagttt gtttacaaac aagccaactt atctattttg
    37261 cacagttctt agagcgtgtt cagttcagct cttgaactaa ataagtatct gaaccccgtg
    37321 aaaactggcc gttctggcct gacaatttaa caggagaaaa taaacatgtc acgtattggt
    37381 aataaagtta tcgtgttgcc tgctggtgtt gaactcgcta acaatgacaa cgttgtaact
    37441 gtaaaaggac ctaaaggaga acttactcgt gagttctcaa aagatattga aatccgtgtg
    37501 gaaggtactg aaataactct tcaccgtcca aacgattcaa aagaaatgaa aactatccac
    37561 ggaactactc gtgccctttt gaacaacatg gttgttggtg tatcagaagg attcaagaaa
    37621 gaacttgaaa tgcgtggggt tggttaccgt gcacagcttc aaggatctaa acttgttttg
    37681 gctgttggta aatctcatcc agacgaagtt gaagctccag aaggaattac ttttgaactt
    37741 ccaaacccaa caacaatcgt tgttagcgga atttcaaaag aagtagttgg tcaaacagct
    37801 gcttacgtac gtagccttcg ttcaccagaa ccatataaag gtaaaggtat ccgttacgtt
    37861 ggtgaattcg ttcgccgtaa agaaggtaaa acaggtaaat aatgttgagt ggttgatcat
    37921 caaccaccaa cctattttcc aactttgtgc atagcacacg atttaaaact aaagaggtga
    37981 aaactgtgat ttcaaaacca gataaaaaca aactccgcca aaaacgccac cgtcgcgttc
    38041 gcggaaaact ctctggaact gctgatcgcc cacgtttgaa cgtattccgt tctaatacag
    38101 gcatctacgc tcaagtgatt gatgacgtag cgggtgtaac gctcgcaagt gcttcaactc
    38161 ttgataaaga agtttcaaaa ggaactaaaa ctgaacaagc cgttgctgtc ggtaaactcg
    38221 ttgcagaacg tgcaaacgct aaaggtattt cagaagtggt gttcgaccgc ggtggatatc
    38281 tatatcacgg acgtgtgaaa gctttggctg atgcagctcg tgaaaacgga ttgaaattct
    38341 aataggagga cactagaaaa tggcatttaa agacaatgca gttgaattag aagaacgcgt
    38401 agttgctgtc aaccgtgtta caaaagttgt taaaggtgga cgtcgtcttc gtttcgcagc
    38461 tcttgttgtt gttggtgacc acaatggtcg cgtaggattt ggtactggta aagctcaaga
    38521 agttccagaa gcaatccgta aagcagtaga tgatgctaag aaaaacttga tcgaagttcc
    38581 tatggttgga acaacaatcc cacacgaagt tctttcagaa ttcggtggag ctaaagtatt
    38641 gttgaaacct gctgtagaag gttctggagt tgccgctggt ggtgcagttc gtgccgttgt
    38701 ggaattggca ggtgtggcag atattacatc taaatcactt ggttctaaca ctccaatcaa
    38761 cattgttcgt gcaactgttg aaggtttgaa acaattgaaa cgcgctgaag aaattgctgc
    38821 ccttcgtggt atttcagttt ctgatttggc ataagaaagg ggataaaatg gctcaaatta
    38881 aaattacttt gactaagtct ccaatcggac gcattccatc acaacgtaaa actgttgtag
    38941 cacttggact tggcaaattg aacagctctg ttattaaaga agataacgct gctatccgtg
    39001 gtatgatcac agcagtatct cacttagtaa cagttgaaga agtaaactaa tgaagtttta
    39061 ggggatgtgc actgtaccat cccctaaaac tagatatagt catctatgat gacatcgtat
    39121 aggcgagttg atgggggaga caaccttttc tcccttatcg gcgctagcat tttacaaaag
    39181 aggagaaaat aaaaatgaaa cttcatgaat tgaaacctgc agaaggttct cgtaaagtac
    39241 gtaaccgcgt tggtcgtggt acttcatcag gtaacggtaa aacatctggt cgtggtcaaa
    39301 aaggtcaaaa agctcgtagc ggtggcggag ttcgccttgg ttttgaaggt ggacaaactc
    39361 cattgttccg tcgtcttcca aaacgtggat tcactaacat caacgctaaa gaatacgcaa
    39421 ttgtgaacct tgaccaattg aacgtctttg aagatggtgc tgaagtaact ccagttgttc
    39481 ttatcgaagc aggaattgtt aaagctgaaa agtcaggtat taaaattctt ggtaacggtg
    39541 agttgactaa gaaattgact gtgaaagcag ctaaattctc taaatcagct gaagaagcta
    39601 tcactgctaa aggtggttca gtagaagtca tctaagagag gtgacctatg ttttttaaat
    39661 tattaagaga agctcttaaa gtcaagcagg ttcgatcaaa aattttattt acaattttta
    39721 tcgttttggt ctttcgtatc ggaactagca ttacagttcc tggtgtgaat gccaatagct
    39781 tgaatgcttt aagtggatta tccttcttaa acatgttgag cttggtgtcg gggaatgccc
    39841 taaaaaactt ttcgattttt gccctaggag ttagtcccta tatcactgct tctattgttg
    39901 tccaactctt gcaaatggat attttaccca agtttgtaga gtggggtaaa caaggggaag
    39961 taggtcgaag aaaattgaat caagctactc gttatattgc tctagttctc gcttttgtgc
    40021 aatctatcgg gattacagct ggttttaata ccttggctgg agctcaattg attaaaactg
    40081 ctttaactcc acaagttttt ctgacgattg gtatcatctt aacagctggt agtatgattg
    40141 tcacttggtt gggtgagcaa attacagata agggatacgg aaacggtgtt tccatgatta
    40201 tctttgccgg gattgtttcc tcaattccag agatgattca gggcatctat gtggactact
    40261 ttgtgaacgt cccaagtagc cgtatcactt catctatcat tttcgtaatc attttgatta
    40321 ttactgtatt gttgattatt tactttacaa cttatgttca acaagcagaa tacaaaattc
    40381 caatccaata tactaaggtt gcacaaggtg ctccatctag ctcttacctt ccgttaaagg
    40441 taaatcctgc tggagttatc cctgttatct ttgccagttc gattactgca gcgcctgcgg
    40501 ctattcttca gtttttgagt gccacaggtc atgattgggc ttgggtaagg gtagcacaag
    40561 agatgttggc aactacttct ccaactggta ttgccatgta tgctttgttg attattctct
    40621 ttacattctt ctatacgttt gtacagatta atcctgaaaa agcagcagag agcctacaaa
    40681 agagtggtgc ctatatccat ggagttcgtc ctggtaaagg tacagaagaa tatatgtcta
    40741 aacttcttcg tcgtcttgca actgttggtt ccctcttcct tggtgtgatt tccattttac
    40801 cgattgcagc taaagatgta tttggtcttt ctgatgttgt tgcctttggt ggaacaagtc
    40861 tcttgatcat tatctctaca ggtatcgaag gaatcaagca attggaaggt tacctattga
    40921 aacgtaagta tgttggtttc atggacagaa cagaataaaa gtatttactg aatcagtaaa
    40981 tgctgaggga gtggaggttt aaactctgac atttgtaaga gttggatctc ccctcttcta
    41041 ttttgttttt aaataggggt gaaaagactt tttgcttcta tttaaaaata aaataaggag
    41101 atcaaatcat gaatcttttg attatgggct tacctggtgc aggtaaggga actcaagcag
    41161 caaaaatcgt agaacaattc catgttgcac atatctcaac aggtgatatg ttccgcgctg
    41221 caatggcaaa tcaaactgaa atgggtgttc ttgctaagtc atatattgac aagggtgaat
    41281 tggttcctga cgaagttaca aatggaatcg taaaagaacg cctttcacaa gatgatatta
    41341 aagaaacagg attcttattg gatggttacc cacgtacaat tgaacaagct catgccttgg
    41401 acaaaacatt ggctgaactt ggcattgaac tagaaggtgt tatcaatatt gaagtgaacc
    41461 ctgacagcct cttggaacgt ttgagtggcc gtatcatcca ccgcgtaact ggagaaactt
    41521 tccacaaggt ctttaaccca ccagttgact ataaagaaga agattactac caacgtgaag
    41581 atgataagcc tgagacagta aaacgtcgtt tggatgttaa tattgctcaa ggagaaccaa
    41641 tcattgctca ctaccgtgcc aaaggtttgg ttcatgacat cgaaggtaat caagatatca
    41701 atgatgtctt ctcagatatt gaaaaagtat tgacaaattt gaaataaagc gtttttcata
    41761 cttgcaaaaa ttcgctacaa atgttatact gagatagtct gacttataat tgttgtctct
    41821 gtgtctagag gcatcgaatc gaaatttatg gaggtgcttt tgcgtggcaa aagacgatgt
    41881 gattgaagtt gaaggcaaag tagttgatac aatgccgaat gcaatgttta cggttgaact
    41941 tgaaaatgga catcagattt tagcaacagt ttctggtaaa attcgtaaaa actatattcg
    42001 tattttagcg ggagatcgtg ttactgtcga aatgagtcca tatgacttga cacgtggacg
    42061 tatcacttac cgctttaaat aatcgaaaaa cttggaggga taagaaatga aagtaagacc
    42121 atcggtcaaa ccaatttgcg aatactgtaa agttattcgt cgtaatggtc gtgttatggt
    42181 aatttgccca gcaaatccaa aacacaaaca acgtcaagga taagatagaa aggagaaaac
    42241 atggctcgta ttgctggagt tgatattcca aatgacaaac gcgtagtaat ctcattgact
    42301 tatgtttatg gtatcggact tgcaacatct aagaaaattt tggctgctgc tggaatctca
    42361 gaagatgttc gtgtacgtga tcttacatca gatcaagaag atgctatccg tcgtgaagtg
    42421 gatgcaatca aagttgaagg tgaccttcgt cgtgaagtaa acttgaacat caaacgtttg
    42481 atggaaatcg gttcataccg tggtatccgt caccgtcgtg gacttcctgt ccgtggacaa
    42541 aacactaaaa acaacgctcg cactcgtaaa ggtaaagctg ttgcgattgc tggtaagaaa
    42601 aaataatata ggaggtaaaa gtcttggcta aaccaacacg taaacgtcgt gtgaaaaaga
    42661 atatcgaatc tggtattgct catattcacg ctacatttaa taacactatt gttatgatta
    42721 ctgatgtgca tggtaatgca attgcttggt catcagctgg tgctcttggt ttcaaaggtt
    42781 ctcgtaaatc tacaccattc gctgctcaaa tggcttctga agctgctgct aaatctgcac
    42841 aagaacacgg tcttaaatca gttgaagtta ctgtaaaagg tccaggttct ggtcgtgagt
    42901 cagctattcg tgcgcttgct gccgctggtc ttgaagtaac agcaattcgt gatgtgactc
    42961 cagtgccaca caatggtgct cgtcctccaa aacgtcgccg tgtataatca tcgcattaca
    43021 ctgcttttcg tttaagaggg agtaactaaa tgatcgagtt tgaaaaacca aatataacaa
    43081 aaattgatga aaataaagat tatggcaagt ttgtaatcga accacttgaa cgtggctacg
    43141 gtacaactct tggtaactct cttcgtcgtg tacttctagc ttctctacca ggagcagctg
    43201 tgacatctat caacattgat ggtgtgttac atgagtttga cacagttcca ggtgttcgtg
    43261 aagacgtgat gcaaatcatt ctgaacatta aaggaattgc agtgaaatcg tacgttgaag
    43321 acgaaaaaat catcgaactg gatgttgaag gtcctgctga agtaacagct ggtgacattt
    43381 tgacagatag cgatattgaa attgtaaatc cagatcatta tctctttaca atcggtgaag
    43441 gttcttctct aaaagcgact atgactgtta acagtggtcg tggatatgta cctgctgatg
    43501 aaaataaaaa ggataatgca ccagttggaa cacttgctgt agattctatt tatacaccag
    43561 ttacaaaagt caactatcaa gtggaacctg ctcgtgtagg tagcaatgat ggtttcgaca
    43621 aattaaccct tgaaatcttg acaaatggaa caattattcc agaagatgct ttagggcttt
    43681 cagcacgtat tttgacagaa catcttgatt tgtttacaaa tcttactgag attgctaagt
    43741 caactgaagt gatgaaagaa gctgatactg aatctgacga ccgtatttta gatcgtacga
    43801 ttgaggaact ggacttgtct gtgcgttcat acaactgttt aaaacgtgcc ggtatcaata
    43861 ctgtgcatga tttgacagaa aaatctgaag cagagatgat gaaagtacga aatcttggac
    43921 gcaagagttt ggaagaagtg aaactcaaac tcattgattt gggtcttgga ttaaaagata
    43981 aataaaggag gaatacatgg cttaccgtaa actaggacgc actagctcac aacgtaaagc
    44041 aatgcttcgc gatttgacaa ctgacctttt gatcaacgaa tcaatcgtga caactgaagc
    44101 tcgtgctaaa gaaatccgta aaactgttga aaaaatgatt actctaggta aacgtggtga
    44161 tttgcatgca cgtcgtcaag cagctgcttt cgtacgtaat gaaatcgcat ctgaaaacta
    44221 tgatgaagca actgataagt acacttctac tacagcactt caaaaattgt tctcagaaat
    44281 cgcacctcgt tatgctgaac gtaacggtgg atacactcgt atccttaaaa ctgaaccacg
    44341 tcgtggtgat gcagcgccaa tggcgatcat cgaattagta taaaatcatc aattttgttg
    44401 agtgttatga tgatggagtc ttgtgctctt agtctagctc tggtctaccg ctaggatttc
    44461 ggtcctagcg ggaacactca tcataagttg ggatagtaga cgcttgttta cgaaattgtt
    44521 tttttcttaa gaacaacttc gtaagcaggc gtttttgagt attttcgtta gaattatgct
    44581 atactatttg aaaagaatcc tgtttaatgt taaggtttct tattttaaga agaattggag
    44641 tttacttatg aaagccatta taactgttgt tggtaaagat aaatctggaa ttgttgcagg
    44701 tgtttctggt aaaattgcag aattaggatt gaatattgac gatatctctc aaactgtatt
    44761 ggatgaatat tttacgatga tggctgttgt atctagtgat gaaaagcaag attttaccta
    44821 tcttcgtaat gaatttgaag cttttgggca aactttgaat gtaaaaatca atattcagag
    44881 tgcagcgatt ttcgaagcta tgtataatat ctaggaggtc atcatggata ttagacaagt
    44941 tactgaaacc atcgccatga ttgaggagca aaacttcgat attagaacca ttaccatggg
    45001 gatttctctt ttggactgta tcgatccaaa tatcaatcgt gctgcggaga aaatctatca
    45061 aaaaattacg acaaaggcgg ctaatttagt agctgttggt gatgaaattg cggctgagtt
    45121 gggaattcct atcgttaata agcgtgtatc ggtgacacct atttctctga ttggggcagc
    45181 gacagatgcg acggactacg tggttctggc aaaagcgctt gataaggctg cgaaagagat
    45241 tggtgtggac tttattggtg gtttttctgc cttagtacaa aaaggttatc aaaagggaga
    45301 tgagattctc atcaattcca ttcctcgcgc tttggctgag acggataagg tctgctcgtc
    45361 agtcaatatc ggctcaacca agtctggtat taatatgacg gctgtggcag atatgggacg
    45421 aattatcaag gaaacagcaa atctttcaga tatgggagtg gccaagttgg ttgtattcgc
    45481 taatgctgtt gaggacaatc catttatggc gggtgccttt catggtgttg gggaagcaga
    45541 tgttatcatc aatgtcggag tttctggtcc tggtgtggtg aaacgtgctt tggaaaaagt
    45601 tcgtggacag agctttgatg tagtagccga aacagttaag aaaactgcct ttaaaatcac
    45661 tcgtatcggt caattggttg gtcaaatggc cagtgagaga ctgggtgtgg agtttggtat
    45721 tgtggacttg agtttggcac caacccctgc ggttggagac tctgtggcac gtgtccttga
    45781 ggaaatgggg ctagaaacag ttggcacgca tggaacgaca gctgccttgg ccctcttgaa
    45841 cgaccaagtt aaaaagggtg gagtgatggc ctgtaaccag gtcggtggtc tatctggtgc
    45901 ctttatccct gtttctgagg atgaaggaat gattgctgca gtgcaaaatg gctctcttaa
    45961 tttagaaaaa ctagaagcta tgacggctat ctgttctgtt ggattggata tgattgccat
    46021 cccagaagat acgcctgctg aaactattgc ggctatgatt gcggatgaag cagcaatcgg
    46081 tgttatcaac atgaaaacaa cagctgttcg tatcattccc aaaggaagag aaggcgatat
    46141 gattgagttt ggtggtctat taggaactgc acctgttatg aaggttaatg gggcttcgtc
    46201 tgtcgacttc atctctcgcg gtggacaaat cccagcacca attcatagtt ttaaaaatta
    46261 agaaaatagg agaaatttta agttctattt aagattagac gtgtatacta taatcattaa
    46321 ataaagacct cctaatatta tttgaaacag ataacactga attagtttga atttgatttt
    46381 catctaatat ctttatttaa tgaactccta aactttttca taataatctc cttcaaaagt
    46441 cgcctgtatg ggtggctttt attttatcat tcatgatata atagaagcaa acggaggacg
    46501 gaaaatggta aaagtacgat tgtatttggt acgtcatggc aagaccatgt ttaacacgat
    46561 tggtcgcgcg caaggttgga gcgatactcc cttaactgct gaaggtgaac gagggattca
    46621 agagttagga attggtttgc gagaatctga tctacagttt gagcgcgctt attcgagtga
    46681 ttctggtcgt accattcaga ccatgggaat tatccttgaa gaacttggct tgcaggggaa
    46741 aatcccttat cgcatggaca agcgtatcag agaatggtgt ttcggtagtt ttgatggagc
    46801 ctatgatggc gatcttttca tgggcattat tcctcgtatc tttaatgtgg accacgttca
    46861 ccaattgtct tatgctgaac tggctgaggg cttggtagag gtcgatacag ctggttgggc
    46921 tgaaggctgg gaaaaactca gtggccgaat caaggaaggc tttgaaatga ttgcaaaaga
    46981 aatggaagat caaggtggag gtaacgccct tgttgtcagc catggaatga ctattggaac
    47041 cattgtttat ctgattaatg gcatgcatcc gcatggtcta gataatggta gcgtgacgat
    47101 tcttgaatat gaggacggtc agtttagggt tgaagttgtc ggtgaccgta gttaccgaga
    47161 gctaggacgt gagaagatgg aagaaggctc tatttaatca gtctagactt gcttgccatg
    47221 agctagggat ttgataagaa tatcaagata agaaaaaaca gccgagggta ctcctttcgg
    47281 ctgtttttga tgtggaaaac taaagtgtaa tgctattgct tttagagatt ttcataaaca
    47341 agagcaagga acctactgtt agaacagtca ggatagttga caaggctgcg gctacaccgt
    47401 aatttcctct gagaacctct gtataaatag ctacagtcat tgttcttgtt ttgacattgt
    47461 agaggaggat agaagtagag agttttgaaa tcattatgac ctaagataag atggctccag
    47521 aaatgatacc agatagcatc attggagttg taatcttagc aaaggtattg agacgactac
    47581 ttcctaagct ttcagcagct tcttcaatac ttggtgctat ttgttgtaag ctagcaacag
    47641 atgagcgaat agtataaggt aatcttctga cagatagaga cataatcaag atgaaagcag
    47701 tccctgtaat cataagaaat ccacttccaa atagaccagt attgaaggaa gaaatgaagg
    47761 caatccctag aacggttcct ggtacaatat aaggtaccat actgaggctg tcaattaagt
    47821 ttgtaaacaa attccgtttt ctaacggcta ggtaggagat aaatgtcgca aatagaacaa
    47881 ctagaactaa ggcaatcaaa gggatacgaa tggtattgaa aatagcagat cccatacgat
    47941 tgaaagctac cttgtaactg tttggagaat aacctttaac agataccata cctgatgttt
    48001 ttaggaaaga ggtataaatt aagtagattt gaggtaaaac agagataaag ataattccgt
    48061 agactgttgc ataaatggca gctatttttc cttttgtagt ttttttaggc tcaattggat
    48121 ggagcagatt catgctgaaa ctgtagcggt ttgcaatgtg tttttggata aggaaaattg
    48181 ccaagacaat gataatcgcc ataattgcaa aagcagaatt tcctccaacc tcgctaataa
    48241 attgggtata aatcaggaca gggaaggtcc gatatccttc gccaatcaac ataggcgttc
    48301 caaagtctga gaatgctctc ataaatacaa gcaaggagct gctagtaagg ttggaactag
    48361 gagaggtaaa acaaccgtta cgataggttt aaatccgaag gaccccatgc tttcagctgc
    48421 ttcaagtaga gaattgtcaa tactgttcat tgttccagca acatatagaa ataccagtgg
    48481 gaatagttgc agtgtaaaga caagtacaat tcctttgaat ccataaatat cgatagctgg
    48541 aagatgaagg gcatttgtca aaaatttagt gatgacccca tttcgtccta gcaagagaac
    48601 ccaggagtag gctcctacga aaggagctga catggaagca atgataatca atatttgtag
    48661 aaatttcttc cccttgaagt catacataga gaagagataa gctaataggg ttcctacaac
    48721 taaggaagtg acagtagcgg taatggaaac cttgaaactg ttgactagtg tctcagagta
    48781 gtagacttta ctaaagaaag tgacaaaatt agctagtgag aattgtcctt catgtataag
    48841 tgcttgcttg agcacggtaa cgataggata aacgagaaag acaagatagg taagaaagat
    48901 gaagaaagag gaggttgtcc aaatatttag ttttttacat tccatggttg actcctttta
    48961 tcaggttttg ggaaccatct gcagaaaaga tgtttaattt ttgcgtattg attcgtagac
    49021 gaatacgatt gcctttttgt agttcttctt caaaagttga ttcttcacta acttgaattt
    49081 ttgaggcaaa acctgtctca atgaaatact ccgtatttag tccaagatag acgctatcgc
    49141 taatagttcc ttcaatatct ccagattcat ctttgataaa ctcttcggga cgaatgctta
    49201 catgaatagc ttgctcctca acctgatcaa gagctggcat tcgaagggca tagccatctg
    49261 aaaagacgat ataagcgccg tcgctccgtt tttcaagatt agtagggata atatttgtgc
    49321 gtccgataaa ggttgccaca aactcattag ctggtttatg atagagttct tttggtcggc
    49381 cgatttgttg gatcacccca tctttcataa cagcaatttg gtctgaaata gccatggctt
    49441 cttcttggtc gtgggttaca taaacagttg taattcccac ttcgtgttgg atttctcgga
    49501 tggcttgacg catatccaag cgaagtttgg cctccagatt actaagtggc tcgtccatga
    49561 ggagaacact tggattaacc gctaaggcgc atgccaaggt gacacgttgt tgttgtccac
    49621 cactgagttt atcgggcttt caatccgcat attgagcaat ttgcatgagt tcaagatact
    49681 tgttggtctg ttgaatcaat tcttcttttg gaaccttctt ttgcataaga ccaaaagcaa
    49741 cgttgtctcg gacagtcaaa tgtgggaaaa tagcgtagtt ttggaaaacc agcccgatat
    49801 tgcgtttgct gggttccata ttattgattt ttgtatcatc gaagtaaaat tctccatctt
    49861 tgatactgtt gaaacctgca atcatacgaa gaagggtcgt tttcccacat cctgaagctc
    49921 caagaagggt aaagagactt ccttttggaa ttgtaatgtt caaattctca ataacaggga
    49981 catcgtggta gatttttttg gcgttaataa tttcgatctc actcatagtg aacctctttt
    50041 actgtttaga ttggatatct gtaaagactt cgttgtattt cttaacgata tctgatttat
    50101 tcttgatgac ataatcataa tcttcagtga gtgttttgat tttgtcaatt ggtttcatgt
    50161 tttcgcttgt tttagcattt ttacgaacag gacggttagt agtggttgta ccaagtgtat
    50221 cttgtacttc ttgagagata ataaaatcga taaatttctt ggcattttcc atatttttag
    50281 cttttttaac gatagcagca ctagcaggta ggaagacggt tccttctttt ggatagacta
    50341 ctttaatgtt agctccgtca tttaagagtt taactgctgg atcctcataa gagagaccaa
    50401 cagccatttc tccatcaacg actgctttat agacaccaga tgaacttgaa ccgattttat
    50461 agtgtattga aactagaata gtacacctct acttctaaaa cattgttaga aatcgatttg
    50521 actgtcctga ttaatttgtc ctgttcttat ttcattttac tataccatca ataagtgtga
    50581 aaagatcttt tacataagac caagccttat catctttgta accaccttga gcttgtagca
    50641 tatttgttaa ttgagcaaag gcgctagaag agtttgctgg gtcagcagtt gcgatttttc
    50701 cttttagttc aggtttgaga aggtcgctat atccttcgat gttcatgcct ttagttaaat
    50761 cagggtggac gattaaaaca ctaccatcta gtgtataagg agtagagaag ccagttgtgt
    50821 tttgatattc tttgataaca ttatcatttt cttttgaaat atagttttca aagagttctc
    50881 cgtgggtagc atattgtgta taagaaccac caaagataac atcagctaca ggaacttctt
    50941 tttctgactc tagttttttg aaaagttctc cagtaccagc ttgaatcagt tctattttga
    51001 taccatattt ttcttcaaag gcaggaatag ttgctccaat taagccttct gagtttggtg
    51061 aataaacgac tagcgaacca ccgtctcctt tatcagatga actgtcatcg gcagattcat
    51121 tagaagaaca agcagcataa tacatccatt tctttttcat gatggatatc tccgttgtgt
    51181 tatttaagtt tattttaaaa caatgtaagc gtttttaaaa cctacaattc tattctatag
    51241 tgtattgaat ctataacagt acactttgac tgctaaaata tttctataaa ttaatttgac
    51301 tttcctgata gagatgttca catcttattt caattcacta tattagagta aaattctcta
    51361 caaaaagaag aatagcctat ttgactattc ttccgagtga tttcaatttc tttggggaaa
    51421 tatggagata ctttttaaat actttggcaa aatatttata gtctgaaaat ccaacttggt
    51481 cagagatagc gcttaaaggg gagttgggag attcagccat cttatctata gcttgtttca
    51541 ggcgatattg taagatatat tcatttagag tcatggggaa gtcttctttg ataaccttat
    51601 atagatgctt gttatctgca aatagctaga gcatttcaag gaaataaact agaaataaca
    51661 attaatatgg tggttcagaa ataccttttg ataatcaata agatcttcag gatgaagtgc
    51721 gttgattcca tccatttcat atttccgatt taataaacga tatttgtttt caccaaattg
    51781 atgaaaaggg agtagttgaa cttggtcgat atttaatgag ttaaatagag tagcgaattt
    51841 ttctgcatcc tctaaactat tgttaaaatt aggaataact gggattctta aaacgatagt
    51901 tttattttga gaaaaagcat aatgaatgtt tttaataatc atttgattaa aaaccccagt
    51961 cactttttta tgttttatag aattataatg ttttaggtct gtgtagataa aatccacata
    52021 ttgaattaaa tcaataaatt tttcatgatc aacaaaggca gtagtttcaa tggcagtgtg
    52081 tatgtgatgt tctttagctg attttaagat ggctttagca aattcaaact gagcaaatat
    52141 ttcacctcct gataaagtta aacctccacc ggattcttcg taaaattctt tgtcttttaa
    52201 tacctctgta ataatttctt ctacagtctt ttcttcaccg actaaggtga atttctctcg
    52261 ttgagcatct ttcatttttt caggtttcat tctttgagat tcaggattag aacaccatgg
    52321 acagcgcaga ggacatcctt ttaaaaaaac agttgtacga atacccgggc cgtcatgaat
    52381 tgaaaagtgt tgaatattaa aaataattcc tttagatatt tccataagag ttcctcctct
    52441 tcataagtct atcctatcac aaaaacgaaa gaaaaacaat tacgaacgaa agtttaattt
    52501 gttgtttttt gctttttatt ttggtagaat aaaacggagg tgtaaaatga aaagattgga
    52561 acaaattatt aaattagtat cagaatatga aaagatcgac gttaatacat tatcggaaaa
    52621 attaaatgta tcgaaagtaa cgattagaaa agatttagat aaattagagt caaaaggttt
    52681 attacacaga gagcatggat atgctgtatt aaatagtgga gatgacttaa atgtacgttt
    52741 gtcaattaac tatgaaatta agagaaaaat tgttcaggaa gcagtaaaat tggtgtcaga
    52801 taatgaaaca ataatgatag aatctggatc gacctgtgct ttacttgctg aggaaatttg
    52861 caagcaaaaa agaaatgtta cgattgtaac aaattcattt tttatagcaa attttgtgag
    52921 agcttatgat tcatgtcgta ttattgttct tggtggagag tttcagaagg attcacaggt
    52981 tactgtggga cctttattaa aagaaatgat acagactttt catgtgcatc aagcttttgt
    53041 tgggacagat ggttacgata aagagatggg ctttacagga aaagatttaa tgcgcagtga
    53101 ggtagttcaa tatatttcag cagcgtcgga taaagtcatt gtactaactg actcaagtaa
    53161 atttgataaa aggggtacag taagaagatt tgctttaagt caagtctatg aagtaataac
    53221 agacgaaaaa ctttctaaac aaaatatagc tacattagaa aatgctggga taatggttaa
    53281 ggtagtttcg taagaggtta aatgtatgaa tcaagatagg aataaactgc tctctaaaat
    53341 tgcttatctg tattatattg aaaacttaaa tcagtcacaa atagcagcaa aattaggaat
    53401 ttatagaacc tctattagta gaatgttaac agaagcaagg aatgcaggaa ttgttaaaat
    53461 tgaaatagag aattttgata ccaatatgtt taagttggaa aattatgtaa aagaaaaata
    53521 tggtctggaa agtttagaaa ttattccaaa tgaatttgat gataatccaa caattttatc
    53581 tgaaagaatt tctcaagttg cagcaggtgt ccttaggaat ctaattgatg ataatatgaa
    53641 aattggcttt tcttggggga aaagtttaag taatttagta gatttaattc acagtaaaag
    53701 tgtccgaaat gttcacttct atcctctagc aggtggtcct agtcacatac acgctaaata
    53761 ccatgtgaat acactgattt atgaaatgtc tagaaaattt catggagagt gtacatttat
    53821 gaatgcaacg attgtgcaag aaaataaatt gttagcagat ggtattttgc aatcaagata
    53881 ttttgaaaat ttgaaaaata gttggaaaga tttagatata gctgtagtcg gaattggtga
    53941 ttttagcaat aaagggaaac atcaatggtt agacatgctt acagaggatg actttaaaga
    54001 attgaccaaa gttaaaactg taggagaaat ttgttgtcga ttttttgatt caaaaggtaa
    54061 agaagtctat gaaaacttac aggaaagaac gatagccatc tctttggaag atttaaaaaa
    54121 tattcctcaa agtttagctg ttgcttacgg tgatacgaaa gtatcttcga ttctttctgt
    54181 cttgcgtgct aatttagtaa atcatttgat tacagataaa aatacaattt taaaagtttt
    54241 ggaagaagat ggtgatttga cttttagaga gattctaggt gagtgaaaat gatagactga
    54301 tcagtttatc gttttttctt tttagtcgat tgcacatttg tgcttatata aacaaaaata
    54361 gtttatctgt tgtttttgga ttgacaactt tattatgtag ttgtattcta tagttacaaa
    54421 agaaaatttt aaaatttcaa atgaaaaaag ctttttacat agtgaaatga ggaggaattt
    54481 atggaaatta ttgttccaga tcaaattatc atgggtttaa ttttatatgc tggcgatgcg
    54541 aaacaacata tttataaagc attagattac ataaaaaatg gtacatgtga acggtgtgaa
    54601 gaagaaatcc agttagctga tgccgcctta ttagaagccc ataatctaca aacaaaattc
    54661 ttggcacaag aagcgtctgg tacaaagaca gaaattacag ctctctttgt tcattcacaa
    54721 gatcatctca tgactagcat gacggagatt aatttaatca aagaaattat tagtttgaga
    54781 aaagaacttc ataaaaaata atactagagt attatcattg ttattaacat agaggaggaa
    54841 aacataatgg tgaagattgg tttgttttgt gcagcaggtt tttctactgg tatgcttgta
    54901 aataatatga aaattgcagc gcaatctagt ggagttgagg cagaaataga ggcgttttct
    54961 cagtctaaat tagcggatta tgcgccaaat atagatgttg cactattggg tccacaagtt
    55021 gcttatacat tagataaatc aaaagaaatt tgtgataagt gtgatgttcc gatagctgtt
    55081 attccgatga tggactatgg tatgttagat gggaaaaaag tattagattt ggccctatct
    55141 ttgattagtg ggtaagaaaa ggagatttat tatgtcaaag atggatgttc agaaaatcat
    55201 tgcaccgatg atgaagtttg tgaatatgcg tggcattata gctctaaaag atgggatgtt
    55261 agcaattttg ccattgacag tagttggcag tttgttcttg attatgggac aattgccgtt
    55321 cgaaggatta aataagagca ttgctagtgt ttttggagct aattggacag agccgtttat
    55381 gcaagtatat tcaggaactt ttgctattat gggtctaatt tcttgttttt caattgccta
    55441 ttcttatgct aagaatagcg gagtagaggc tttaccagct ggagttctat ctgtatctgc
    55501 attctttatt ttgctaagat catcttatat ccctaaacaa ggtgaggcga ttggggacgc
    55561 tattagtaaa gtttggtttg gaggccaagg aattatcggt gctatcatta taggtttggt
    55621 agtaggaagt atttatacct tctttataaa gagaaaaatt gttattaaga tgccagaaca
    55681 agttccacaa gctattgcca aacagtttga agcaatgatt ccagcatttg taattttctt
    55741 atcttctatg attgtatata ttttagcgaa gtcattgact aatggcggaa cattcataga
    55801 aatgatttat tctgctattc aagttccgtt gcaaggttta actggatctt tgtatggtgc
    55861 tattggaatt gcattcttta tatcattttt gtggtggttt ggtgttcatg ggcaatcggt
    55921 agtaaatgga gtagtgacag ctctgctttt atctaatctt gatgctaata aagctatgtt
    55981 agcctctggt aatctatcat tagaaaatgg tgcacatatt gttactcaac aatttttaga
    56041 ttcattttta attctatcag gttcagggat tacgtttggt cttgtagttg ccatgctttt
    56101 tgcagcaaaa tcaaaacaat accaagcctt aggaaaagtt gcagcttttc cagcaatatt
    56161 taacgtaaat gagccagttg tatttggatt tccgattgtc atgaatccag ttatgtttgt
    56221 acctttcatt cttgttcctg tacttgcagc tgtgatagta tatggagcta ttgcaacagg
    56281 tttcatgcaa ccattctcag gggtaacatt gccttggagt acaccagcta ttctatcagg
    56341 atttttggtg ggtggatggc aaggtgttgt gactcagtta ttagtattgg cgatgtctac
    56401 aatggtttac tttccattct ttaagataca agatcgttta gcttgccaaa atgaaatcaa
    56461 acaatcttag aggtatttgt gtgttactgt taaactcaca catttgtgct aaaaattaga
    56521 gagttaaaat ttttctagtt aaaagcttga aaatttctat aaaaatcggt attatatttt
    56581 cgaaagaaat aaaaatattt tcgaaagaaa ggtgcttacg atggtaaata cagaagtagc
    56641 aagaacaaca atcaagacag aatattttgg cagccttact gaaaggatga acaaatatcg
    56701 agaagatgtt ttaaataaaa aaccttatat tgatgctgag agagcagttc tagcaacacg
    56761 cgcctatgaa cgatacaagg aacaacctaa tgtcctaaaa cgtgcatata tgctgaaaga
    56821 aattttggaa aatatgtcta tctatattga agaagaatct atgattgcgg gaaatcaagc
    56881 ttcttccaat aaagatgctc ctatttttcc ggaatatacg ctagaatttg ttctcaatga
    56941 gttggatctt tttgaaaagc gtgatggaga tgttttctat attacagaag aaacaaaaga
    57001 acaacttaga agtattgctc cgttttggga aaataataat ttacgtgcta gagctggtgc
    57061 cttattacct gaagaagtgt ctgtttatat ggaaacagga ttcttcggta tggaaggtaa
    57121 gatgaattct ggagatgctc acttagcagt taactatcag aaacttttgc aatttggttt
    57181 aagaggtttt gaagagcggg ctcgtaaagc gaaggtagct ctagatttaa cagatccagc
    57241 aagtattgat aaatatcatt tttacgactc tatatttatc gtaatcgatg ctattaaagt
    57301 atatgcaaag cgctttgttg ctcttgctaa aagtttagcc gaaaatgcaa atcctaaacg
    57361 taagaaagaa ttacttgaga ttgcagatat ttgctctaga gtcccatatg aaccggcaac
    57421 tacttttgca gaagctattc aatcagtttg gtttattcaa tgtattttac aaattgaatc
    57481 taatggccac tctctttcat atggccgttt tgatcaatat atgtatccat atatgaaggc
    57541 tgatttagaa agtggtaaag aaacagaaga tagcattgtt gaacgtctga caaatctttg
    57601 gattaagaca attacaatta ataaggttcg cagtcaatca catacatttt cttcagcagg
    57661 aagtccttta tatcaaaatg ttacaattgg tggacagact cgagataaga aggatgctgt
    57721 taacccatta tcttatttgg tattaaaatc agttgcacaa acccatctac cgcaacctaa
    57781 tctaactgta cgttaccatg caggtttaga tgctcgtttc atgaatgagt gtattgaagt
    57841 gatgaaactt ggttttggta tgcctgcatt taataatgat gagattatta ttccttcttt
    57901 tattgcaaaa ggagtattgg aagatgatgc ttatgattac agtgccattg gatgtgttga
    57961 aacggcagtt ccagggaaat ggggctatcg ttgcacaggt atgagttata tgaacttccc
    58021 taaggttcta cttatcacga tgaatgatgg aattgatccg gcttcgggta aacggtttgc
    58081 accaagcttt ggtcatttta aggatatgaa gaacttttct gaattagaaa atgcttggga
    58141 taaaacacta agatatttga cacgaatgag tgttattgtt gaaaattcta ttgatttatc
    58201 attggaacga gaagttcctg atattctatg ttcagcattg actgatgatt gtattggtcg
    58261 tggaaaacac cttaaagaag gtggagcagt atatgattat atatcaggat tgcaagttgg
    58321 aattgcaaat ttgtcggatt cattagctgc aattaaaaaa ttggtgtttg aggaagaacg
    58381 tataagccca agtcagcttt ggcatgcact ggaaacagat tatgccggag aagaaggtaa
    58441 ggtcattcaa gaaatgttga ttcatgatgc acctaagtat ggtaatgatg atgattatgc
    58501 tgacaaattg gttactgctg cttatgacat ttatgttgat gaaattgcta aatatcctaa
    58561 tacacgttat ggaagaggtc caattggagg aattcgttat tcaggaacat cttctatctc
    58621 agccaacgta gggcagggac gtggaacatt agcaactcca gatggacgca acgcgggtac
    58681 accgttagca gagggttgtt caccatcaca taatatggat caacacggcc ctacatctgt
    58741 tttaaaatct gtttcaaaat taccaacaga tgaaatcgta ggtggggttc tcttaaatca
    58801 gaaagtaaat cctcaaacgt tagccaaaga agaagataaa ttaaaactaa ttgctttgtt
    58861 acgaacattc tttaatcgtt tacatgggta ccatattcaa tacaatgttg tttccagaga
    58921 gacgctgatt gacgctcaga aacatcctga aaaacacaga gacttaattg ttcgtgttgc
    58981 aggatactct gcattcttca atgttctttc taaggcaacc caagatgaca ttataggacg
    59041 tactgagcat actttgtaaa ataaagaggt tctttttatg gaatttatgc ttgacacatt
    59101 aaatttagat gagattaaaa agtggtctga aattttgccg ctagctgggg taacttcaaa
    59161 tcccactatt gcaaaaagag agggttctat taattttttt gaacgaatca aagatgtaag
    59221 agaattgatt ggctctacac cctctattca tgttcaggtg atttctcaag attttgaagg
    59281 catcttaaag gatgctcata aaattcgaag acaagcagga gatgatatat ttatcaaagt
    59341 acctgttact ccagctggat tacgtgcaat aaaggcgcta aaaaaagagg gctaccatat
    59401 cactgcaaca gctatttata cagttattca gggattatta gctatcgaag caggagcgga
    59461 ttacctagct ccatattata atagaatgga aaatctgaac attgattcaa attctgtcat
    59521 tcgtcaatta gctcttgcta ttgatagaca gaactctcct agtaagattt tagctgcatc
    59581 ctttaaaaat gtagcacaag taaataatgc tttagctgca ggtgcgcatg ctgttacagc
    59641 aggagcggat gtttttgaat cagctttcgc catgccatct atccaaaagg cggttgatga
    59701 tttttctgac gattggtttg ttattcaaaa tagtcgttcc atttagatag agaggaaata
    59761 catatgagaa tttttgctag tccttctaga tatattcagg gggaaaatgc cttgtttgaa
    59821 aatgccaaat caattttgga tttgggaaat tgccctattc tattatgcga tcagttggtt
    59881 tatgatattg ttggaaaacg atttgaagat tacctacata ggtatggttt ccatattgtt
    59941 ctggcgctat ttaatggtga agcttctgac aatgaaatca atcgagttgt tgccttggct
    60001 gagaaagaaa attgtgatag tattatcggt cttggtgggg gaaagacgat tgatagcgca
    60061 aaagctattg cagatttgat tgaaaagcct gttattattg ctccaacaat tgcatcgacc
    60121 gacgcacctg tatctgcttt atctgttatt tatacagatg aaggtgcatt tgatcattat
    60181 ctattttatt ctaaaaatcc agatttagtt ttggttgata caaaagttat ttcacaagcc
    60241 cctaagcgtt tattagcgtc tggtattgca gatggtttag caacttgggt tgaggcgcgt
    60301 gcggttatgc aggcaaatgg aaaaactatg ttgggacaac agcaaacatt ggctggagtt
    60361 gcaattgcga agaaatgtga agaaacgctg tttgcagatg gtttacaggc tatggcagct
    60421 tgtgaagcta aagtggtgac accagcatta gaaaatattg ttgaagctaa tactttattg
    60481 agtggtctag gttttgaaag tggaggatta gctgcggcgc atgcaattca taatggtttt
    60541 actgcattga caggtgacat tcatcattta acacatggtg aaaaagtagc ttatggaact
    60601 ttagtacaac tattattgga aaatagacct aaagaagaac ttgataagta tattgagttt
    60661 tacaaaaaaa ttggtatgcc aacaactcta aaagaaatgc atttggatca agttggatat
    60721 gatgatttaa taaaagttgg taaacaagca actatggagg gtgagacaat tcatcagatg
    60781 ccgtttaaga tttcgccttc agatgttgct caagctatta tcgctgtaga tgcctatgta
    60841 aattcaaaat aaacaataag gactactgtt ttccaaatgg tagtctttta ttgatccctg
    60901 tattgaattc tatagaagat tgaaatagga tgagaacaaa tcgattggga aagtaaaatt
    60961 aatttctata aatgttttag caattgtttc gtactatttc agattcagtc tactatatgt
    61021 tcttcataaa tcaaaaagcg acataggttg tcggcaatca aaaagcgaca taggttgtcg
    61081 gctatttatt gtgaatacat taattagcat tccagtttta tcttcggtct aaaataagta
    61141 ttttgtgcta tacgagataa gcttcttgac ttactccttg atttactgca taacaatggg
    61201 ataaaaagtg ggagatagag caattcatag tcatcaaaat taatgagata cagtatacag
    61261 tttttccttt aaacacattt caaattccct caaaaatggt ataatagtaa catcacaaaa
    61321 ttggagagag accatgagtt tttacaatca taaagaaatt gagcctaagt ggcagggcta
    61381 ctgggcagaa catcatacat ttaagacagg aacagatgca tcaaaaccta agttttatgc
    61441 gcttgatatg ttcccttatc cgtctggagc tggtctgcac gtaggacacc cagaaggtta
    61501 tactgcaacc gatatcctca gtcgttacaa acgtgcgcaa ggctacaatg tccttcaccc
    61561 aatgggttgg gatgcttttg gtttgcctgc agagcaatac gctatggata ctggtaatga
    61621 cccagcagaa tttacagcgg aaaacattgc caacttcaaa cgtcaaatta atgcgcttgg
    61681 attttcttat gactgggatc gtgaagtcaa cacaacagat ccaaactact acaagtggac
    61741 tcaatggatt ttcaccaagc tttacgaaaa aggcttggcc tatgaagctg aagtgccagt
    61801 aaactgggtt gaggaattgg gaactgccat tgccaatgaa gaagtgcttc ctgacggaac
    61861 ttctgagcgt ggaggctatc cagttgtccg caaaccaatg cgccaatgga tgctcaaaat
    61921 cacggcttac gcagagcgct tgctcaatga cttagatgaa ctagattggt cagagtctat
    61981 caaggatatg caacgcaact ggattggtaa atcaactggt gccaatgtaa ctttcaaagt
    62041 aaaaggaaca gacaaggaat ttacagtctt tactactcgt ccggacacac ttttcggtgc
    62101 gactttcact gtcttggctc ctgaacatga attagtagac gctatcacaa gttcagagca
    62161 agcagaagct gtagcagact ataaacacca agccagcctt aagtctgact tggctcgtac
    62221 agaccttgct aaagaaaaaa caggggtttg gactggtgct tatgccatca accctgtcaa
    62281 tggtaaggaa atgccaatct ggattgcaga ctatgtcctt gctagttatg gaacaggtgc
    62341 ggttatggct gtgcctgccc acgaccaacg tgactgggaa tttgccaaac aatttgacct
    62401 tccaatcgtc gaagtacttg aaggtggaaa tgtcgaagaa gctgcctaca cagaggatgg
    62461 cctgcatgtc aattcagact tcctagatgg actcaacaaa gaagacgcta ttgccaagat
    62521 tgtggcttgg ttggaagaaa aaggctgtgg tcaggagaag gttacctacc gtctccgcga
    62581 ctggctcttt agccgtcaac gttactgggg tgagccaatt ccaatcattc attgggaaga
    62641 tggaacttca acagctgttc ctgaaactga attgccgctt gtcttgcctg taaccaagga
    62701 tatccgtcct tcaggtactg gtgaaagtcc actagctaac ttgacagatt ggcttgaagt
    62761 gactcgtgaa gatggtgtca aaggtcgtcg tgaaaccaac actatgccac aatgggctgg
    62821 ttcaagctgg tactacctcc gctatattga tccacacaat actgagaagt tggctgatga
    62881 ggacctcctc aaacaatggt tgccagtaga tatctacgtg ggtggtgcag agcatgctgt
    62941 tcttcacttg ctttacgctc gtttctggca caaattcctc tatgacctcg gtgttgttcc
    63001 gactaaggaa ccattccaaa aactctttaa ccaagggatg attttgggaa caagctaccg
    63061 tgaccaccgt ggtgctcttg tggcaaccga caaggttgaa aaacgtgatg gttccttctt
    63121 ccatgtagaa acaggggaag agttggagca agcgccagcc aagatgtcta aatcgctcaa
    63181 gaacgttgtt aacccagacg atgtggtgga acaatacggt gccgataccc ttcgtgttta
    63241 tgaaatgttt atgggaccac tcgatgcttc gattgcttgg tcagaagaag gtttggaagg
    63301 aagccgtaag ttccttgacc gagtttaccg tttgattaca agtaaagaaa tccttgcgga
    63361 aaacaatggt gctcttgaca aggtttacaa cgaaacagtc aaagctgtta ctgagcaaat
    63421 tgagtctctc aaattcaaca cagctattgc ccaacttatg gtctttgtca atgctgctaa
    63481 caaggaagat aagctttatg ttgactatgc caaaggcttt attcaattga ttgcaccatt
    63541 tgcacctcac ttggcagaag aactctggca aacagtcgca gaaacaggtg agtcaatctc
    63601 ttatgtagct tggccaactt gggacgaaag taaactggtt gaagacgaaa tcgaaatcgt
    63661 tgtccaaatc aaaggtaaag tccgtgccaa actcatggtc gctaaagacc tatcacgtga
    63721 agaattgcaa gaaattgctc tagcggatga aaaagtcaaa gcagaaattg acggtaagga
    63781 aatcgtgaaa gtgattagtg taccaaataa attggttaat attgttgtaa aataaccaat
    63841 ttatttgctg atttccaacc tcaaacagtt tcccaaactg tttgagccaa ctaaactcgt
    63901 taatatcgtt gtgaaataag gtaggaatcc ttcagagtag aatctggagg attttttgaa
    63961 tcttcttatg aaagtatggt atactatggg caactataaa gtttgaaaag tgaaaaaagg
    64021 agaaagctat gccagtaaat gaatatggtc aaatgattgg ggagtcaatg gaaggttata
    64081 caccaggtga actgccttct attgatttct tagaagggcg ttatgctagg atagaggctc
    64141 tttcagtgga aaagcatgcg gaggatttgc tagctgttta tgggcctgat acgcctcagg
    64201 agatgtggac ctaccttttt caggagccag tagcagatat gggggagctg gttagccttt
    64261 tacatcagat gttggctcgt aaggaccgtt tttactatgc aatcatagac aaggcgactg
    64321 gtaaggcttt gggatctttt tctctcatgc gcattgacca gaataaccga gtaatagaag
    64381 tcggagcagt cactttttct ccagagctaa ggggtacacg gataggaact gaagctcagt
    64441 atctcctagc tcgctatgtt tttgaggagc taaactatcg tcgctacgag tggaaatgcg
    64501 atgccttaaa tctgccatcc agacgagctg cggagcgttt gagctttatt tatgaaggaa
    64561 ccttccgtca ggcagtggtt tataagggac gtacgagaga tacggattgg ttgtctatga
    64621 ttgataagga ctggcctcaa gtcaaagatc gtttggaaac atggttgcgt cctgaaaatt
    64681 ttgataaaaa tggacagcag tacaagagct tgagagaact ttaagaggtg ttgacatgat
    64741 tactattaaa aagcaagaaa ttgtcaagct agaggatgtt ttacatctct atcaggctgt
    64801 cggttggaca aattataccc atcaaccaga gatgctggag caggccttat ctcattcatt
    64861 agtaatttat ctggcacttg atggtgatgc tgtggtgggc ttgattcgtt tggttggaga
    64921 tggattctca tcagtattgg ttcaggattt aatcgtttta ccaatctatc agcgtcaagg
    64981 aattggtagt gccttgatga aagaggcttt agaggattac aaagatgcct atcaagtcca
    65041 gctggtgaca gaagagacag aaagaacctt gggcttctat cgttctatgg gctttgaaat
    65101 cttatccacc tataattgta tagggatgac ttggatgaat cgtaaaaaat aacaaaactt
    65161 gttttttctt aagcaaaatt taaggatggt ctagtattat atagtcatta aataaagacc
    65221 tcctaacttt atttaataaa atcctaaact tttttcatca caatctccta atgaagccac
    65281 ccaatcaggt ggcttttttg cgtggtgagg tgctggtgat agaaattttt tgcaaaatag
    65341 taaaatttta gaaaagttaa gctagt
//
LOCUS       DAVKQB010000005        56923 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKQB010000005 DAVKQB010000000
VERSION     DAVKQB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 56923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.005"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(99..632)
                     /gene="queF"
                     /locus_tag="VW528_000291"
     CDS             complement(99..632)
                     /gene="queF"
                     /locus_tag="VW528_000291"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9911178.1"
                     /translation="MKRQSALVVFSGGQDSKTCLFWTMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(939..1253)
                     /gene="trxA"
                     /locus_tag="VW528_000292"
     CDS             complement(939..1253)
                     /gene="trxA"
                     /locus_tag="VW528_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9911179.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            complement(1270..1491)
                     /locus_tag="VW528_000293"
     CDS             complement(1270..1491)
                     /locus_tag="VW528_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9911180.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            complement(1590..1970)
                     /locus_tag="VW528_000294"
     CDS             complement(1590..1970)
                     /locus_tag="VW528_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9911181.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            complement(2115..3068)
                     /locus_tag="VW528_000295"
     CDS             complement(2115..3068)
                     /locus_tag="VW528_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9911182.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(3136..4344)
                     /gene="sstT"
                     /locus_tag="VW528_000296"
                     /pseudo
     CDS             complement(3136..4344)
                     /gene="sstT"
                     /locus_tag="VW528_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            4483..5043
                     /locus_tag="VW528_000297"
     CDS             4483..5043
                     /locus_tag="VW528_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU9911183.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGNRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            5100..6008
                     /locus_tag="VW528_000298"
     CDS             5100..6008
                     /locus_tag="VW528_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9911184.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            5960..6550
                     /locus_tag="VW528_000299"
     CDS             5960..6550
                     /locus_tag="VW528_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9911185.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            6553..7659
                     /gene="yqeH"
                     /locus_tag="VW528_000300"
     CDS             6553..7659
                     /gene="yqeH"
                     /locus_tag="VW528_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9911186.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            7873..8184
                     /gene="yhbY"
                     /locus_tag="VW528_000301"
     CDS             7873..8184
                     /gene="yhbY"
                     /locus_tag="VW528_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9911187.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            8236..8865
                     /locus_tag="VW528_000302"
     CDS             8236..8865
                     /locus_tag="VW528_000302"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9911188.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVEYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            8865..9458
                     /gene="yqeK"
                     /locus_tag="VW528_000303"
     CDS             8865..9458
                     /gene="yqeK"
                     /locus_tag="VW528_000303"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9911189.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVGKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            9459..9959
                     /locus_tag="VW528_000304"
     CDS             9459..9959
                     /locus_tag="VW528_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9911190.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEEHFADVL
                     DYKHIF"
     gene            9972..10325
                     /gene="rsfS"
                     /locus_tag="VW528_000305"
     CDS             9972..10325
                     /gene="rsfS"
                     /locus_tag="VW528_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9911191.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            10428..11174
                     /locus_tag="VW528_000306"
     CDS             10428..11174
                     /locus_tag="VW528_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9911192.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKEKKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            11184..12281
                     /locus_tag="VW528_000307"
     CDS             11184..12281
                     /locus_tag="VW528_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9911193.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVNILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTARSVEELVELVTTKRY
                     TKARVRRLLTYILMQARENVLPEVIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGNPSIADQNFGRVPIRIETN"
     gene            12519..12773
                     /locus_tag="VW528_000308"
     CDS             12519..12773
                     /locus_tag="VW528_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU9911194.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            12777..13031
                     /locus_tag="VW528_000309"
     CDS             12777..13031
                     /locus_tag="VW528_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU9911195.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            13222..14835
                     /locus_tag="VW528_000310"
     CDS             13222..14835
                     /locus_tag="VW528_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9911196.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            14965..15591
                     /gene="gmk"
                     /locus_tag="VW528_000311"
     CDS             14965..15591
                     /gene="gmk"
                     /locus_tag="VW528_000311"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9911197.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            15616..15930
                     /locus_tag="VW528_000312"
     CDS             15616..15930
                     /locus_tag="VW528_000312"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9911198.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            15996..18392
                     /locus_tag="VW528_000313"
     CDS             15996..18392
                     /locus_tag="VW528_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9911199.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQVRILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTRNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            18405..19340
                     /locus_tag="VW528_000314"
     CDS             18405..19340
                     /locus_tag="VW528_000314"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9911200.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPNRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSICKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            19333..20646
                     /gene="rsmB"
                     /locus_tag="VW528_000315"
     CDS             19333..20646
                     /gene="rsmB"
                     /locus_tag="VW528_000315"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9911201.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVALKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFANGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            20661..21401
                     /locus_tag="VW528_000316"
     CDS             20661..21401
                     /locus_tag="VW528_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9911202.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            21398..23377
                     /gene="pknB"
                     /locus_tag="VW528_000317"
     CDS             21398..23377
                     /gene="pknB"
                     /locus_tag="VW528_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9911203.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            23492..24250
                     /locus_tag="VW528_000318"
     CDS             23492..24250
                     /locus_tag="VW528_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9911204.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            24252..24701
                     /locus_tag="VW528_000319"
     CDS             24252..24701
                     /locus_tag="VW528_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9911205.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            24763..25389
                     /locus_tag="VW528_000320"
                     /pseudo
     CDS             24763..25389
                     /locus_tag="VW528_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            25457..25705
                     /locus_tag="VW528_000321"
     CDS             25457..25705
                     /locus_tag="VW528_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911206.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            25836..27008
                     /locus_tag="VW528_000322"
     CDS             25836..27008
                     /locus_tag="VW528_000322"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9911207.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTVLSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            27008..28282
                     /locus_tag="VW528_000323"
     CDS             27008..28282
                     /locus_tag="VW528_000323"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9911208.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQDDPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVEQLIADKTFNLETAQRYLENLRS"
     gene            complement(28318..29283)
                     /locus_tag="VW528_000324"
     CDS             complement(28318..29283)
                     /locus_tag="VW528_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9911209.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVTTTGYFLPVTLLPGKSI"
     gene            complement(29264..30718)
                     /locus_tag="VW528_000325"
     CDS             complement(29264..30718)
                     /locus_tag="VW528_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9911210.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            31094..32977
                     /locus_tag="VW528_000326"
     CDS             31094..32977
                     /locus_tag="VW528_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9911211.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFIIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFETKV
                     AQGDKVKVGDVLGTFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            33126..34013
                     /locus_tag="VW528_000327"
     CDS             33126..34013
                     /locus_tag="VW528_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9911212.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(34056..34673)
                     /locus_tag="VW528_000328"
     CDS             complement(34056..34673)
                     /locus_tag="VW528_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9911213.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(35088..35276)
                     /locus_tag="VW528_000329"
                     /pseudo
     CDS             complement(35088..35276)
                     /locus_tag="VW528_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(35786..35866)
                     /locus_tag="VW528_000330"
                     /pseudo
     CDS             complement(35786..35866)
                     /locus_tag="VW528_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            35982..36875
                     /locus_tag="VW528_000331"
     CDS             35982..36875
                     /locus_tag="VW528_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911214.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            36872..38071
                     /locus_tag="VW528_000332"
     CDS             36872..38071
                     /locus_tag="VW528_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9911215.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLEQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(38424..39191)
                     /locus_tag="VW528_000333"
     CDS             complement(38424..39191)
                     /locus_tag="VW528_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911216.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(39208..39903)
                     /locus_tag="VW528_000334"
     CDS             complement(39208..39903)
                     /locus_tag="VW528_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911217.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(39908..40273)
                     /locus_tag="VW528_000335"
     CDS             complement(39908..40273)
                     /locus_tag="VW528_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9911218.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            40413..40886
                     /gene="nrdR"
                     /locus_tag="VW528_000336"
     CDS             40413..40886
                     /gene="nrdR"
                     /locus_tag="VW528_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9911219.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            40887..42056
                     /locus_tag="VW528_000337"
     CDS             40887..42056
                     /locus_tag="VW528_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9911220.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFNPKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPDYKNETSEETRLELERKKQELLARLEKGGD"
     gene            42057..42953
                     /gene="dnaI"
                     /locus_tag="VW528_000338"
     CDS             42057..42953
                     /gene="dnaI"
                     /locus_tag="VW528_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9911221.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            42950..43663
                     /locus_tag="VW528_000339"
     CDS             42950..43663
                     /locus_tag="VW528_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9911222.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAKMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            43677..44987
                     /gene="der"
                     /locus_tag="VW528_000340"
     CDS             43677..44987
                     /gene="der"
                     /locus_tag="VW528_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9911223.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            45078..45413
                     /locus_tag="VW528_000341"
     CDS             45078..45413
                     /locus_tag="VW528_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911224.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            45553..45888
                     /locus_tag="VW528_000342"
     CDS             45553..45888
                     /locus_tag="VW528_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911225.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            46073..46864
                     /locus_tag="VW528_000343"
     CDS             46073..46864
                     /locus_tag="VW528_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911226.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILHVVPYMVLKKYEDNV"
     gene            46857..47498
                     /locus_tag="VW528_000344"
     CDS             46857..47498
                     /locus_tag="VW528_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911227.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            47502..48152
                     /locus_tag="VW528_000345"
     CDS             47502..48152
                     /locus_tag="VW528_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9911228.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            48163..48450
                     /locus_tag="VW528_000346"
     CDS             48163..48450
                     /locus_tag="VW528_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911229.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     MQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            48596..51109
                     /gene="secA"
                     /locus_tag="VW528_000347"
     CDS             48596..51109
                     /gene="secA"
                     /locus_tag="VW528_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9911230.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMATLTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQAMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            51190..52221
                     /locus_tag="VW528_000348"
     CDS             51190..52221
                     /locus_tag="VW528_000348"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9911231.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            52223..53254
                     /locus_tag="VW528_000349"
     CDS             52223..53254
                     /locus_tag="VW528_000349"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9911232.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            53293..53661
                     /locus_tag="VW528_000350"
     CDS             53293..53661
                     /locus_tag="VW528_000350"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9911233.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            53651..54754
                     /locus_tag="VW528_000351"
     CDS             53651..54754
                     /locus_tag="VW528_000351"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9911234.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLALPYDLIPALTLESALVHIKTVPAGACMGYGATYQTDSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            54773..56788
                     /gene="recG"
                     /locus_tag="VW528_000352"
     CDS             54773..56788
                     /gene="recG"
                     /locus_tag="VW528_000352"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9911235.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHYMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIETWQEDPEWRMIALHLEKKEHLD"
BASE COUNT        16828 a        10959 c        12989 g        16147 t
ORIGIN      
        1 agctgacttc gtcagtttta tctacaacct caaacactgc tttgagtaac ctgcggctaa
       61 cttcctagtt tgctctttga ttttcattga gtatgacttt agcggttgtc aattttctct
      121 ggataaaggt cgtgttggaa gaggcgttgt tctgccaagc cctcatactt agttccttgc
      181 ttaccgtagt tatagtaggg gtcgattgaa atgccaccgc gcggagtgaa ttttccccag
      241 acttctaaat agcgagggtc tagcaagttg accaagtctt tcccgatggt gttgatacag
      301 ttttcgtgga aatctccgtg gtttcggtag ctaaatagat atagtttgag ggattttgac
      361 tcgacacaga gcttgtcagg aatgtaggaa atatgaatcg tcgcaaagtc tggctgagca
      421 gtgatttgtc ccagcagaga catatcgagg atatggtgac gaatgccctg ttccttagcg
      481 atttctctag taatttgaat ttcgaggtga tgacgttggc cgtaggcaaa ggtgacagct
      541 tcgactgttt catagtgttg catggtccag aaaaggcagg tttttgaatc ttgaccacca
      601 ctaaagacga ccaaggctga ttgacgtttc atagtactcc ttccaaaatg ggaaatgttc
      661 agagcacgta aaaagctccc attagggagc taaaaaatac caaatcgagg ttttttttag
      721 cgatggcata tcccaaatat cgtaatattc tatttatata gtaaaatgaa ataagaacag
      781 gacaaatcga tcaggacagt caaatcgatt tctaacaatg ttttagaagt agagatgtac
      841 tattctagtt tcaatctact atagtctagc atatcttttg aaaaatggca aagggcaaga
      901 aaaaagagac caaagaaagt acttggtctc tcgtttgatt agctcaattc agcaatgatg
      961 gccttgattt gttctgctgt gtgaacacct gcaacttgtt tgacaacttg gccgtctttt
     1021 ttgaagagaa gagttggaat agacatgatt ccaaaagcac gagctgtgtt tggattttca
     1081 tcaacgtcca ttttaacgat tttcaagaca tcttctgaaa gttcttcaga caatttgtcc
     1141 aagattggac cttgcatacg acatggacca caccaagttg cccagaagtc tactaagact
     1201 aaaccgtctt ttgtttcttg ttcgaatgtt gcatctgtaa ttgcttttgc cattgtattt
     1261 ctcctttttt tagttatatt ggcttaaatc ttgtttcatg agatagaaga agatatctcc
     1321 ataagtccca tggtagtcca aattatgacc cttgtaagtt aatttttgga cagggtagta
     1381 gtctgcgacg ccgataaggc aagcttgttg cgaacgttca aagtcttcat aagactcgaa
     1441 agtcacagtt ctttcgttct tgctggcatc tatataggta atttcaatca tgtttaaaac
     1501 tcctttgttt aatgctaact ttattttact ccttataaaa gagaatgtca agaaaaatga
     1561 ttgcgcacgc aacttttttt aaaatcatct taaatcaaga aatccaaacc tgcttccaag
     1621 ctttcttcga cagtcttttg tagcgaggcc agtgtctttt gcccatcatt tgtcaggcag
     1681 ataaaactag agcgtctatc ttgatggcaa cacatgcgac tgagtagacc gcaattttta
     1741 gcttccaagc gagccaccat cctagaaact gcgctcgggc tcagatgaag cttatctggc
     1801 aggtcaatct ggcgtagaga tttttcttca gccaagtcca gatagtagag caggtagaac
     1861 tctttcaagg tcagactttg ctcgctctgt tgggcaatgg tctcttccaa gagactttca
     1921 atttctttct gacgccgatt gaagtcaaac catttttcca aataggtcat agtgtctcct
     1981 ttctttttag agtcataatt atagaagaaa gtccattaac gggcagtctt tgcgtcataa
     2041 gatgattgcg catgcaataa ttatactact tttcaagaat gctggcaagc tctgtttttt
     2101 agtggttttc ttttttactg tctatatttt tggtaaaaat caacttttac ttggatgaag
     2161 gttttggctt cacgtaggag ttgaagaaga gtggcgcggg tttcaaactc ttctcttgtc
     2221 ttgggcagac tgcggttccg gaagacttcc agataacgtt caatttcatc tagcaaatca
     2281 gaagcaggat tggtctggct cagttgacct gcaatttttg aaaagagttg cgctaagatc
     2341 aggctttcac tggcggcaag gtgacaagtg ttaatctgtt gggccatgtt tctcaggata
     2401 cgactttgtc gttgtctcat ctcaaagtag tggatatggt agtcggtctg gtgaaagagg
     2461 tggtcagagt gatccaaata gaccagtctg agggcttctt tcaaaagcgt gtctaattct
     2521 gctaccagct gtgctcggtt gcgtccgtct cctctggata aatagtattt gaagcgctgg
     2581 aggatatctt ttaacttttc ttccaccagc gtgtggtagt gctggatttc ctcttctcgt
     2641 gaaggcatat agagattaac aagcaaggca aatcctgtac caatagcaaa gagaaggaat
     2701 tcgttgacta ggaggtctgg agaggttgac tcttgaacca agagatggct aaccaaaacc
     2761 gtgcttggtg tgatgccaat ttcccagccc atcttgtagg ctaaaggaac gtagaaggcc
     2821 agatagaggc cgagactcca gatatgaaat ccgctcaagt gaaaagctag aacgccgata
     2881 gccagagcta gaagcataga aaaaagacga ttgcgagcca gttttaaagt acttctacgc
     2941 gtatcagata ggctcaagag agcgataatt ccagccgaaa ctgctgacga aagattgaga
     3001 aaataagcaa gcaggcaggc aagacaggta gctaagatga gcttggtcgt acgttggcta
     3061 atagacataa gaatttccta ataagttaga ataaaagcgt aaaagacaag acatgagcag
     3121 gcttgccttg atgagttatt ttttacgggt tgctgcgtat tcggcaacgg cggtaaagag
     3181 gacatctgta gaagagttaa gggctgtttc acatgagtct tggatgacac caatcacaaa
     3241 accaacccca acaatttgta tggcaatatc gttagaaata ccgaaaaggc tacaagcaac
     3301 tgggataaga aggagggaac ctccggcaat acctgaagca tcacaggctg agatagctgc
     3361 taccacactg aggacaaagg ctgtggcaaa gtcaacagga attccaagag tgttaactgt
     3421 aacaagggtc aaaacgttaa tggtaatcgc tactccagcc atattgatag tagaaccgag
     3481 tgggatagaa acagaatagg tatctgggtt gagtccaagg tcatggcaga gtttcatgtt
     3541 gacaggaatg ttagtcgcag aactacgagt gaaaaaggct gtcacaccgc tgacacggag
     3601 gcagttccaa actagagggt aaggattgcg tctcataaag aagaaggcaa tcaaagggtt
     3661 gaccacaggg gcaacaaaaa gcatagtcgt tactaataga accaataaaa taccgtagtt
     3721 ggcaaggctt ccgactccct tgtcagaaat ggttttaaaa acaagaccaa ggattccaaa
     3781 tggagccaga ttgatgatcc attcgacaat tttagaagtc acgtcagcga tagtttttag
     3841 caattcttta ctatttttac tggcttctct catagcgatt ccaaaaatga ctgcccaaga
     3901 taagattcta atatagttag cagtaagcag ggcgttgact gggttgtcaa ccagtttgag
     3961 caagaggttg ctgaggacct gcccaatccc atctggtggt gcaatttcag tattggcact
     4021 atttagggta atttcaatag ggacgatgaa acttgctagt acagctacaa gagcagcggc
     4081 gaaagtccct atcataggat atacaagaaa acaacagttt tcatattgct atcttgtccc
     4141 ttttgatgtt ggaaagggca ttggcaacga gggcaaagac taggatagga gcaacagctt
     4201 ttagacctcc aacgaataaa tccccgagta gcccaatccc tgagagatta ggaagggtca
     4261 gtcctaggat tccccacaaa gcataccaat caagatacgc ttgacaaggc ttgccttatt
     4321 ccaagcatga atgattcttt tcataataat ctcctttttg tgtagtgatt atgattatag
     4381 tataaatgat agacaaaatc aagaattttc tgtctatttt ttgaatattt atggagaatg
     4441 agactgatga aaatatggta taatgaaata aaggagtttt atatgcaaaa atttattcag
     4501 gcttatattg aaaagctaga tgtgacaacc attatcgaga atattctaac caaggtcatt
     4561 tctcttttac tgcttttgat tgtattttat attgctaaaa aaatgcttca taccatggtg
     4621 cagagaattg tcaaaccttc tctaaaaatg tctcgtcatg atgtcggacg ccaaaaaacc
     4681 atctcacgtt tactagaaaa tgtgtttaat tatacgctat atttcttttt actctactgc
     4741 attttgtcga ttttaggttt gccagtttct agtttgctgg ctggagctgg tattgctggg
     4801 gtagcgattg gtatgggagc ccaaggcttt ctgtctgatg tcatcaatgg ctttttcatc
     4861 ctctttgaac gtcaactgga tgtgggagat gaggtcgttc tgacaaatgg accgattact
     4921 gtatcgggta aggttgtcag tgtgggaaat cgtacgacac agcttcgtag cgaggagcaa
     4981 gcccttcact ttgtccctaa ccgaaatatc acagttgtta gcaatttctc acgcacagac
     5041 tagacctgtt attttaagta atttgtggta caatagaggg agtttaataa ggagaaaaga
     5101 tggttttaga aaagcagttg ggcaatggtt gtacctggat agacctagac ctaggaaagt
     5161 tgaataaact agaagacctt tctgaaattt acggtttgga caaggaaacc attgaatacg
     5221 cactggatag aaacgagcgc gcccacatgg attaccaccg tgaaagtggg acggttacct
     5281 ttatctataa tgtcctagac gtaaaaaagg acaaggccta ctatgagact tttcccatga
     5341 cctttattgt cgagcatcgt cgcctgatta ccattagtaa taccaagaac gcctatgtca
     5401 ttgaacagat gactcgttat ctggagagcc atgacacgct ttcgatttat aagtttctct
     5461 ttgccagtct ggaaattatc agcaatgcct actatcctgt cattgagcag atggacaaga
     5521 gtagggatga ggtcaatgac ctcttgcgcc agcgaactac caagaaaaac ctctttgccc
     5581 tgtctgattt ggagactggt atggtttatc tgacggcagc tgccaaacaa aatcggattt
     5641 tgttagagca tattcaaggt catgccttgt atcgtagttt tgatgagatt gagagagaac
     5701 agtttgatga tgccatgatt gaggctcatc agctggtatc catgacagac ctaatctctc
     5761 agattttaca gcagctttca gcctcttaca acaatattct aaacaataat ctgaatgaca
     5821 atttgacaac cttgactatc atttcagtct tgctagctgt tttggcagtc gtgacaggct
     5881 ttttcggaat gaatgttccc ttacctttaa cagatgagcc ccatgcttgg ctctatatca
     5941 gtttggctag tgcaggtttg tggattgttt tatccttgtt actaaggaaa attgcgaaaa
     6001 aaagttaaga aaaggagcca gaatggcgat tgaaaattat ataccagatt ttgctgtgga
     6061 agcagtctat gatctgacag tcccaagcct gcaggcgcag ggaatcaagg ctgttttggt
     6121 cgatttggat aataccctca ttgcttggaa caaccctgat ggaacgccag agatgaagca
     6181 atggctacat gaccttcggg acgcgggtat tggcattatc gtagtgtcaa ataacaccaa
     6241 aaaacgcgtt caacgagcag ttgagaaatt tgggattgat tacgtttact gggccttgaa
     6301 gcccttcaca tttggtattg accgtgctat gaaggaattc cactatgaca aaaaggaagt
     6361 ggtcatggtt ggcgaccagc tcatgacaga tatacgagca gcccaccgtg cagggattcg
     6421 gtcaatttta gtcaaaccct tggtccaaca tgactcaatc aaaacgcaga ttaaccgaac
     6481 tcgtgagcgt cgtgttatga gaaaaatcac tgaaaagtac ggaccgatta catataaaaa
     6541 aggaatttaa ctatggaaga aattctctgt attggttgtg gagcaaccat tcagacgaca
     6601 gataaagctg gtcttggttt taccccccag tcggcacttg aaaaaggttt ggagactggc
     6661 gaagtctatt gccaacgctg tttccgtctc cgccactaca atgaaatcac agatgtccag
     6721 ttgacgaacg atgatttcct caagctcttg cacgaggtgg gagacagtga tgccttagtg
     6781 gtcaatgtca ttgatatctt tgattttaat ggatctgtca tcccaggttt accacgtttc
     6841 gtctcgggca atgatgtcct cttggtagga aataaaaaag atatccttcc taagtcagtt
     6901 aagtctggta agattagcca gtggctcatg aaacgtgccc atgaagaagg tcttcgtcca
     6961 gtcgatgtgg tcctaacttc agcacaaaat aaacatgcca ttaaggaagt cattgacaag
     7021 attgaacact accgtaaggg ccgcgatgtc tatgtggtcg gtgtgaccaa cgttggaaaa
     7081 tcaactctaa tcaatgctat tatccaagaa atcacgggtg atcagaatgt catcactact
     7141 tcacgcttcc caggtacaac cttggacaaa atagagattc cgcttgacga cggatcttat
     7201 atttacgata cgccgggaat tatccaccgc caccagatgg ctcactactt gacggccaaa
     7261 aacctcaagt atgtcagtcc taaaaaggaa atcaagccta agacctatca gcttaatcct
     7321 gagcaaaccc tatttttagg tggtttggga cgctttgact ttatagcagg agaaaagcaa
     7381 ggatttactg ctttctttga taatgaactc aaactccatc gtagcaagct tgaaggagct
     7441 agtgctttct acgataagca cctgggaact cttctgacac caccaaatag caaggaaaaa
     7501 gaagatttcc caaggctagt ccagcatgtc tttaccatta aagataagac agacctagtc
     7561 atctcaggcc taggctggat tcgtgtaaca ggcatagcaa aagtcgccgt ctgggcacca
     7621 gaaggcgtcg ccgtcgtcac acgaaaagca attatttaaa acaaaatcgc tgagcgaaca
     7681 aagtgagctc atatagcgat agttattgcc gtggtgtgat tgccaatctg tagattggca
     7741 aggggcacaa ttgagaccaa ggctcaattg tgaggtcagg aaatccattt ttcaaagatg
     7801 aaatctttgg aaaattagtt ccgaccgtcc ctcaccacct aaaataacta tccaaaaaca
     7861 aggaagaaaa ttatgtcatt aacatcaaaa caacgtgcct tcctcaacag ccaggcacac
     7921 accctcaaac ctatcatcca aatcgggaaa aatggactca acgaccaaat caaaaccagc
     7981 gtccgtcaag ctcttgatgc gcgtgaatta atcaaggtta ctctcttaca aaacacagat
     8041 gaaaacatcc acgaagtagc tgaaattttg gaagaagaaa tcggtgtgga tacagtccaa
     8101 aaaataggac gcatcttgat tttgtttaaa caatctagca agaaagaaaa tcgcaagatt
     8161 tctaagaaag tcaaagaaat ctaagaccga aactccaaat aactgttttt atagagaaat
     8221 aaaggggact agcctatgac aatcgaacta ttgactccct ttaccaaggt agagttggag
     8281 ccagaaatca aggagaaaaa acgcaaacaa gttgggattt taggggggaa ttttaaccct
     8341 gttcacaatg cccatctcat tgttgcggat caagtacggc aacagttggg actggatcaa
     8401 gttcttctca tgcctgaata ccaacctcct cacgttgata aaaaggaaac catccctgaa
     8461 caccatcgtc tcaagatgct tgagttggca attgagggaa ttgacggcct agtcattgaa
     8521 accattgagt tggagcgcaa gggtatttcc tacacctacg acaccatgaa gattttgaca
     8581 gagaagaatc cagatacgga ttattacttt atcatcggtg ccgacatggt tgagtatctg
     8641 cctaagtggt accgaattga tgaactggtt gacatggttc agtttgtggg ggttcagcgt
     8701 ccacgctaca aggtagggac ttcctatcca gttatctggg tggacgtacc gctcatggat
     8761 atctcgtcca gcatggtgcg ggacttcctt gcccaaggtc ggaaacccaa ctttctccta
     8821 cctcagccag tgctagacta catcgagaag gaggggctct actgatgtcc tatcaagact
     8881 atatcaactg ctcccgtgag gccttgttgg aaaaaatggc agagcttcta cctgagaaac
     8941 gtctaaccca ttgtctaggt gtggagcgtg ccgctatgga attagctcag cgctttggtg
     9001 tagatgttgg gaaagcaagt ctagcaggtc ttcttcatga ctatgctaaa aagttgtcaa
     9061 atcaggaatt tctagccttg attgaccgtt accagctaga ccctgacctc aaaaactggg
     9121 gcaataatgt ctggcatggt atggtcggaa tttacaaaat tcaggaagat ttggatttgc
     9181 atgattctga aattctgcga gccattgaaa ttcatacagt cggagctggt caaatgacag
     9241 acctagataa ggtcatctac gtcgcagact atatcgagca caatcgagcc tttcctggag
     9301 tagatgtggc gcgtgagatt gcaagtctat cgctcaataa ggcggtggcc tacgaaacag
     9361 ctcgtaccgt ggagtatcta gctcatcagg gattccccat ctatccccaa acccttgaaa
     9421 cctataacgc ctttgtgcac tatttgaaag aggactaaat gaagacggct tttataatca
     9481 ttgatgttca gaatgtttta gtagaaactg ggttccagac aaaaagtcta ttggaaaaaa
     9541 tttcttattt acaaaaccag gctagaagca agaatattga aattatctat gttcaacata
     9601 ttgagaattc tgaagctcaa acatcagaag attggcagtt atctgcgctt ttaaatcgaa
     9661 aacctgctga aaaggtcttt cagaagaagt ataacagtat tttcaaagaa actggcttaa
     9721 aagaatactt ggataaacag gggattgaaa aattagtttt atgtggtatg cagacagaat
     9781 attgtgtgga tacctctgtc aaggttgcct ttgaatatgg ctatcagctt attgttccag
     9841 aaggtgctgt cacaaccttt gatggggatg acattccagc agaaacgatt aatgaatttt
     9901 atgaggacat ttgggaggag cactttgcag atgtcctaga ttacaaacat attttctaaa
     9961 aagaggatta aatgaacgaa aaagaattac tagaactagt cgtgaaagcg gctgatgaga
    10021 aacgtgcgga ggatatcctc gcacttgatg tacaagattt gactagtgtg acggactact
    10081 ttgtcatcac tagctcaatg aatagccgtc agttggacgc tatcgcagct aatatccgtg
    10141 aaaaagtagc tcaagcgggc tttaaaggta gccatgtcga aggcgatgca gctggaggct
    10201 gggtcttact ggacctcggt gctgttgtcg tgcatatctt ttcagaagaa atgcgtgccc
    10261 attataatct agagaagcta tggcatgagg cgaattcagt agatatttca gaagctcttg
    10321 cttagaagat gaactcagtt gtagtcaact gggtttcttt gcttatttta gaaaaattga
    10381 tagaaaggta aagggctagt ggtgtttacc atggagctct tagtatcatg attatggcga
    10441 cttatgaaac ctttgcggcg gtctatgatg cggtcatgga cgatagttta tacgacaaat
    10501 ggacgaattt ttctctgcgt catttgccta agaccaagga gaaaaagaaa ctcttggaac
    10561 tggcttgtgg gacaggaatt caatcagtgc gcttctctca ggctggtttt gatgtgactg
    10621 gacttgactt gagtgcggat atgttgaaga ttgcggagaa gagagcaact tcagccaagc
    10681 aaaagattgc ttttatagaa ggtaacatgc tgaatttgtc caaggcaggg aaatacgatt
    10741 ttgtcacgtg ttattcggac tctatctgct atatgcagga tgaggtggaa gtaggggacg
    10801 tctttaagga cgtgtacaat gcgctgaatg aagagggagt tttcatcttt gacgtgcact
    10861 cgacctacca gacagatgaa gtattccctg gctattccta ccatgaaaat gcggaagatt
    10921 ttgccatgct ttgggataca tatgaggacg aagctcctca ctccatcgtg catgagctga
    10981 ctttctttat caaggaggct gacggttcct ttagtcgcca cgatgaagta catgaggaga
    11041 ggacctatga gatcttgacc tatgatatct tactggaaca ggctggattc aagtccttca
    11101 aactctatgc agactttgag gataaggaac caacagaaac tagcacccgt tggttttttg
    11161 tggcgcagaa gtaggagatt agcatgacca tcacaggtat tatcgcggag ttcaatcctt
    11221 ttcataatgg ccataaatac ctgctggatc aggcagaggg actgaaaatt gttgccatgt
    11281 cagggaattt catgcagcgt ggagagcctg ctattgtgga taagtggaca agggcccaga
    11341 tggcgctgga aaatggcgca gacttggtag tggaattgcc ctttttagtc agtgttcagg
    11401 cagctgattt ctttggtcaa ggagctgtga atatcttgga tcgcttgggc attgatagtc
    11461 tagtttttgg gacagaggaa gttcgagatt atcagaaaat cgctgactta tacacagaga
    11521 aaggtgctga gatggagaaa tttgtggaaa atctgcctga ttccctttcc tatccccaga
    11581 aaacccaagc tatgtggaag gaatttgctg gtcttgattt ttcaggtaat acccccaatc
    11641 atgttcttgc tctggcctat gccaaggcgg ttgcaggacg aaatatcaag ctgcatccga
    11701 ttcagcgtca gggggcaggt taccattctg tgaataaaga tgtggacttt gcctcggcga
    11761 cagccctccg tcaacatcag aaggaccaag atttcttaga acgctttatg ccttctgttg
    11821 ccctatttga gcaggccagt aaggtgatct gggaagacta ttttcccttg ctccgctatc
    11881 aaatcttgtc aaatcccgac ctaaccacca tctatcaggt caatcaagaa atggctgtgc
    11941 gcattaaaga agctattaaa acagcccggt ctgtggaaga attggtcgag ttagtgacta
    12001 ccaaacgtta caccaaggcg cgtgtcagac gcctattgac ctatattttg atgcaggcta
    12061 gagaaaatgt cttgccagaa gtcattcatg tccttggatt tactgaaaaa ggtaggcaac
    12121 atctcaagtc tttgaaaggt caggtcagtc tagtcagccg aattggcaaa gaaccttggg
    12181 atgctatgac ccaaaaagca gaccagattt accaactagg aaatccaagt atagcagacc
    12241 agaattttgg aagagtgccg attagaatag aaacaaacta agtctaccga aaggtaggct
    12301 ttttgagatt ttttacgaat aatactcttc gaaaatcaaa ttcaaatcgc gtcaacgtcg
    12361 ccttgccgta ctcaagtaca gcctgcggct agtttcctag tttgctcttt gattttcatt
    12421 gagtataaat agatagaaaa gaaaatcttg tacaagttcc tgacaatttc tttcttttcg
    12481 tgtataatag tggaaaagag ctaaaacgag gtatggttat ggaagcagtc ctttactcaa
    12541 cattccgaaa tcatttaaag gactacatga agaaggtaaa tgatgaattt gagcctttga
    12601 cggtggtcaa taaaaatcca gatgaggaca ttgtagtcct ttcaaagagt gagtgggata
    12661 gtatccaaga aaccctgaga attgctcaaa ataaggaact ttctgataag gttttgcgag
    12721 gaatggctca agttcgtgct ggaagtactc aggtccatgt tattgaggag taagaaatgc
    12781 tgctcaagtt tacagaagat gcctgggcag attattgcta ctggcaaaat caggataaga
    12841 aaacgttaaa aagaatcaat aaactaatca aggatattca acgtgatccc tttacaggaa
    12901 taggtaaacc agaaccactc aaatatgatt accaaggagc ctggtcacgg cgtattgatg
    12961 cagaaaatcg cttgatttat atgatggatg gagatagcgt ggctttcttg tcctttaaag
    13021 atcattacta agtctattga aaggtaggct ttttctcaag tctctactaa actactattt
    13081 ctaaacaaca agtttatttt tgcaaatgtg ttccaaattc cccaaaacat attctatttt
    13141 aggtttgtga aaatcacttt tttatggtag aatgtaaaag aatgtatgtc attcggaata
    13201 acaataaaat gaggtaaaaa catggaaatc atgtcgcttg cgattgctgt ttttgccgtc
    13261 atcattggtt tagtcattgg atatgtcagc atctcagcta agatgaaatc atctcaggaa
    13321 gctgcagagt tgatgctttt aaatgctgaa caagaagcaa ctaatttacg tggacaagct
    13381 gagcgtgaag cggatttact tgttaatgaa gccaaacgtg aaagcaagtc tcttaaaaaa
    13441 gaagcactat tggaggccaa agaagaagcc agaaaatacc gtgaagaagt ggacgctgaa
    13501 ttcaaatcag aacgtcaaga actcaaacaa atcgaaagtc gtttgacaga gagagctact
    13561 agccttgacc gtaaggacga caatttgacg agtaaagaac aaacacttga acaaaaagaa
    13621 caaagtattt ctgatagagc gaaaaacctt gatgcgcgtg aagagcaatt agaggaagtc
    13681 gaaagacaaa aagaagcaga actagagcgt attggtgctc tgtctcaggc agaagcacga
    13741 gatattatct tggctcagac agaggaaaac ttgaccaggg agattgccag tcgcattcgc
    13801 gaagctgagc aagaggtcaa ggaacgttct gacaaaatgg ccaaggacat cttggttcaa
    13861 gctatgcaac gtatcgctgg tgaatatgta gcggagtcaa caaactcaac ggttcatctg
    13921 ccagacgata ctatgaaggg acgcattatt ggtcgtgaag gtcgtaacat tcgtaccttt
    13981 gaaagtttga caggggtcga tgtgattatc gacgatacac cagaagtggt gaccttgtca
    14041 ggatttgatc cgattcgtcg tgagattgcc cgtatgacta tggaaatgtt gctcaaagat
    14101 ggtcgtatac atccagctcg tatcgaagag ttggttgaga aaaaccgtca agagattgac
    14161 aataagattc gtgaatacgg tgaggctgct gcctatgaaa ttggtgcgcc aaaccttcat
    14221 ccagacttga tgaagattat gggacgtttg cagttccgta cttcatatgg acaaaatgtt
    14281 ttgcgccatt cgattgaggt tgctaagttg gctggtatca tggcgagcga acttggtgaa
    14341 aatgcggctc ttgcccgtcg tgctggattc cttcacgata tcgggaaagc cattgaccat
    14401 gaggttgaag gtagccacgt tgaaatcggt atggaattgg cccgtaagta caaggaaccc
    14461 ccagttgtgg tgaatacgat tgctagtcac cacggagatg ttgaagctga gagcgtgata
    14521 gcagttatcg tcgctgcagc agatgccttg agcgcagccc gtccaggtgc tcgtagtgag
    14581 tctcttgaaa gctacatcaa gcgtctccat gatttggaag aaattgctaa cggctttgaa
    14641 ggagtgcaaa ctagctttgc ccttcaagca ggacgtgaaa ttcgtatcat ggtcaatcca
    14701 ggaaaaatca aggacgacaa agtcacaatc ttggctcaca aagttcgtaa gaaaattgaa
    14761 aacaatctcg attatccagg aaatatcaag gtaaccgtga ttcgcgagct tcgtgcagta
    14821 gattatgcta aataaataag aaagagcagt tgaaaaatta ctgctttttt gttacactag
    14881 atagaaagac tgtaaaggat aatctggctt tttgccatta ttctagagaa atgttatttc
    14941 acagattgac taaaaggaga cacaatggca gaccgaggct tactaatcgt tttttctggt
    15001 ccttcagggg ttggaaaagg aacggttaga agagagattt ttgagagttc tgaaaaccaa
    15061 tttcaatact ctgtatcgat gacgacacgc gcacaacgtc ctggagaagt ggacggtgtc
    15121 gactatttct tccgtactcg tgaagaattt gaagagctga ttcgtcaagg acagatgttg
    15181 gaatacgcag aatatgtcgg taactactat ggaactcctc tgacctatgt caatgaaacc
    15241 ttggacaagg gaatcgatgt tttccttgaa attgaagttc agggtgctct tcaggtcaag
    15301 aaaaaggttc cagatgctgt ctttatcttc ctgacaccac cagatttgga tgaattgcaa
    15361 gatcgcttgg taggtcgtgg aacagatagt gcagaagtga ttgcccaacg aatcgaaaag
    15421 gccaaggaag aaattgccct catgcgtgag tatgattatg ccattgtcaa cgatcaggtg
    15481 cccctagctg ctgaacgtgt caaatgtgta attgaagcag aacacttctg tgtggatcgt
    15541 gtcattggtc actatcagga gatgttacca aaatctccaa ctacccgata aaatttagaa
    15601 aataggtaca agcaaatgat gttaaaaccc tctattgata ccttgctcga caaggttcct
    15661 tcaaaatatt cactcgtaat cttggaagca aaacgtgccc acgaattgga agcaggtgcc
    15721 ccagcaactc aaggtttcaa gtctgaaaaa tcaactcttc gcgctttaga agaaatcgaa
    15781 tcaggaaacg ttacaattca cccagatcca gaaggaaaac gcgaagcagt gcgtcgccgt
    15841 atcgaagaag aaaaacgccg caaagaagaa gaagaaaaga aaatcaaaga gcaaattgct
    15901 aaagaaaaag aagatggtga aaaaatttaa ggttgggggg actcaatctt attttttcta
    15961 ttgcaagaat gtactgacaa ggaggaggtg agaagatggc cctagctaag attatcgtag
    16021 atgtgccctt gatgcagacg gaccagccct atagttacag gattccggag gaatttgagg
    16081 gaatgctgga agttgggatg cgggttcatg tgccttttgg taagggtaat cgcctgattc
    16141 aagggattgt tcttggtttg gagtcccaat cagatggaga agagatggag caagatttaa
    16201 aagatattgc agaggtgctg gatttttctc ctgttctcac gccagaacaa ctctggctgg
    16261 ctgaggagtt acgcaaatct gtcttctcct acaaaatttc tatcctcaag gccatgcttc
    16321 caggatttct gaattctagc tatgacaaga ttctctatcc tctggaaggc ttgagtcagg
    16381 aagaacgagt gcgcctgttt ggttcagaag attcgctagc cttttcttca ctagaccttg
    16441 ccaagcaagc tgaaatgatg cgtttgacta gaaaaggcct gcttggtctg gaatatcagg
    16501 cagtcgatca aaagaaggtc aagacccagt cttggtatga ggttgaccat gctcaattag
    16561 aaggtgttga gatttctaca cgtgccaaga aaaagttgga actgagagat tatctgctgt
    16621 ctcatccaga gagcgcttcc ttggctagct tgttagagtc ctactcgcga gagcaagtca
    16681 acttctttgt ggatcaaggt gctgttacca tagtccaaaa ggaagttcaa cgctcggctg
    16741 cttattttga aggaattgaa gcaagtagac ctttggagtt aaatccagaa caaagacagg
    16801 cgcgtgatgc ggttgtgagt tctattggca gttctcaacc tccctttcta cttcaaggga
    16861 ttacaggaag tgggaagacc gaggtttact tgcagattat ccaaggtgcc ctggataagg
    16921 gcaagacagc tattttgctg gtacctgaga tttccttgac tcctcagatg accgagcgtt
    16981 ttatagcacg ttttggggac aaggtagcca ttcttcactc aggattatcc aatggtgaaa
    17041 agtatgatga atggcgcaag gtggagcgtg gcgatgccca agttgttgtt ggtgccagat
    17101 ctgctatctt tgctcctctg aaaaatctgg gtgtcatgat tattgatgaa gagcatgaag
    17161 cggcttataa gcaggacagc aatccccgtt accatgccag agaggtagct attttacggg
    17221 ctcagtataa tcaagcaact ctggtacttg gatctgcaac gccgagctta gagagccggg
    17281 cacgtgctgg caaaggcgtc tatcaacact tacgtctaac ccaacgtgcc aatcctttgg
    17341 ctacaatccc tgaggttcaa gtgattgact ttcgagacta tatcggacaa aatgagacgt
    17401 caaactttac gcctcctttg ctagaggcta tccaagaccg tctggttaaa aaagagcagg
    17461 tggttctcat gctcaatcgc cgtggttatt ctagctttgt catgtgtcgg gagtgtggga
    17521 cggtggatac ttgtcccaac tgcgatattt ctctgacctt gcatatggat accaagacta
    17581 tgaactgcca ttattgtggt ttctcgaagg atattcctca ggtctgtcct aactgtaaga
    17641 gccacagtat tcgttactat gggacgggaa ctcagaaggc ttatgatgag ctagcagaac
    17701 tctttcccca agttcgcatt ttgaggatgg atgtggatac gactcgaaag aaaggcagtc
    17761 accaagcctt gcttgaccag tttgggcgag gggaagcgga tattttactt ggtactcaga
    17821 tgattgcaaa gggtttggat tttcccaatg ttaccctagt aggagttcta aatgcggata
    17881 cggccttgaa tctgcctgat ttccgttctt ctgagagaac cttccagctc ttgactcagg
    17941 tggcaggtcg agcaggtcgg gctgaaaaag caggtcaagt cttgatacag tcctacaatt
    18001 cgcagcacta tgctattcga tttgccaagg atcaagatta cgaaggcttt tatgcctatg
    18061 aaatgggaat cagacgacaa ctcggctatc caccttacta tttcacgatt ggtattaccc
    18121 tttctcacaa gaaagaagaa gaggttttca aacgtgccta tgaagtcatg aacattttgc
    18181 ggtcaggctt gtcagagacc agtcccattc tggggccaac gccaaaaccg attgcccgta
    18241 cccgcaacct ctatcattac cagattttaa ttaaataccg tttagaagat gagctggggc
    18301 cgaccctcaa ccaggtcttg gccttgactc aagaacggga aaatagtgag ctccgtctca
    18361 gcattgacca tgagccgcag caatttttat aagaaggaga agatatgaca aaactaatct
    18421 ttatggggac ccccgacttt tcagcaacag tcttaaaagg acttttgaca gatgaccgtt
    18481 acgaaattct agccgttgtg acccagccaa accgtgctgt tggtcgtaaa aaagttatcc
    18541 aagaaacccc agtcaagcag gctgccaagg aagcaggact atctatctac caacctgaaa
    18601 aattatctgg aagtccagag atggaagatc ttatgaagct aggagcagat ggaattgtga
    18661 ctgctgcttt tgggcagttt ctcccaagca aactccttga tagcatggac tttgctgtca
    18721 acgttcatgc ctccctcctt cctagacacc gtggtggtgc gcctatccat tatgccttga
    18781 ttcaagggga tgaggaagct ggtgtgacca tcatggaaat ggttaaggaa atggatgcag
    18841 gagatatgat ttctcgtcgc agcattccga tcacagatga ggacaatgtt ggcaccttgt
    18901 ttgaaaaatt ggcgctagtt ggtcgtgatt tgcttttgga cactctgcct gcctatattg
    18961 ctggtgatat caaacctgaa ccgcaggata cgagtcaggt taccttctct ccaaatataa
    19021 agccagagga agaaaaactg gactggaaca aaaccaatcg tcaactcttt aaccaaattc
    19081 gtggaatgaa cccctggcct gttgcccata ctttccttaa gggcgaccgc tttaagattt
    19141 atgaagccct accagtagaa ggtcagggaa atccaggtga gattctctct atctgcaaga
    19201 aagaattgat tgtcgcaacg gctgaagggg ctctatccct caaacaagtg cagccagctg
    19261 gtaagcctaa gatggacatt gcttccttcc tcaacggagt tggacgtaca ttgactgtag
    19321 gagaacgatt tggtgactaa agtagaaacg gctagaagtt tagctctagc agtgctagag
    19381 gatgtttttg tgaatcaagc atattcaaat attgccttaa ataaacacct caaggggagt
    19441 cagctctctg cagcagacaa gggcttagtg accgagctag tctatggaac ggtagccctt
    19501 aaactgactc tggaatggta cctatcccac tttatcgaag acagagacca gttagacagc
    19561 tggctctatg tccttcttct catgagtgcc taccaactcc gctatttgga caagattcca
    19621 gatcatgctg tggtcaatga agcagtggaa ttggccaaac tccgtaaaaa aggcagtgaa
    19681 aaattggtca acgctgtcct tcgccgtatc ttgcgtgaag gctggccaga tattgctagc
    19741 atcaagcgaa aaaacaagcg tgactccatt gcctattctc tcccagtttg gctagttgcc
    19801 aaactcaagg aagaatacgg agaggagaga gcaaaagcca tctttgaaag cctgttagtg
    19861 cgaaacaaag ccagtattcg tgtaacagac ctaagtcgaa aagaggaaat ccaagccttg
    19921 ttggaggcga ataattccct tttagcaacc actggtctgg ttaaggagca ggggcatttt
    19981 gcagggcata atttgtttgc gaatggagcc attaccatcc aagatgagtc cagtcagctg
    20041 gttgctccga cgcttgattt acaaggtgat gagcgagtgc ttgatgcctg tgcggctcca
    20101 ggtgggaaaa cagcccatat agcctcttat cttacgacag gtcaggttac tgctctggac
    20161 ttgtacgacc acaagttgga tttaattcaa gaaaatgccc aacgtctggg agttgcagat
    20221 cgggttcaaa ctcaaaaatt ggatgccaga aaggtgcatg agttttttga ccaggattcc
    20281 tttgataaga ttttggtgga tgctccttgt tcaggaatcg gtcttttgcg ccgaaaacca
    20341 gatatcaaat acaataaaga aacggcagat ttcgcgtcct tgcaggaaat tcagttagaa
    20401 atattaggta gtgtttgtca aatactaggc aaaggtggta taataactta tagcacctgc
    20461 actatcgtct cagaggagaa ttttcaagtc gttaaggcct ttttagagag tcatcccgag
    20521 ttcgagcagg taaaactaga acatgaatgt aaggatatca tgaaagacgg ctgtatcctc
    20581 attacacctg aattgtatgg aagtgatgga ttcttcatca gtcaatttcg caagatatcg
    20641 gattaggaag gaactgacac atggaaattt cattattaac agatgttggt cagaaacgaa
    20701 caaataacca agactatgtc aaccactatg tcaatagagc tggacgtacc atgattattt
    20761 tagctgatgg gatgggaggt catcgcgcag ggaatatcgc tagtgaaatg gcggtcacag
    20821 acctgggtgt agcttgggtt gatacccaga tcgatacagt caatgaagtg cgtgaatggt
    20881 tcgcccatta cctagaaatt gaaaatcaaa agattcacca gcttggtcag gatgaagctt
    20941 acagaggcat gggaactact ttggaagtcc ttgctattat tgataatcag gctatctatg
    21001 ctcatattgg tgattcgcgt atcggcttga ttcgtggaga agaataccat cagttgacga
    21061 gtgatcattc tttggttaat gaattgctca aggctggtca attgacacca gaagaggcag
    21121 aagctcatcc gcaaaaaaat attatcaccc agtctattgg gcaaaaagat gaaattcagc
    21181 ctgattttgg gacagttatc cttgagtcag gtgactatct cttgctcaat agtgacggct
    21241 tgaccaacat gatttcaggc agtgagattc gtgatattgt aaccagtgat attcctttag
    21301 cagataaaac ggagacactt gttcgttttg ctaacaatgc aggaggttta gacaacatta
    21361 cggttgccct tgtttctatg aacgaggagg atgaagaatg atccaaatcg gcaagatttt
    21421 tgccggacgc tatcggattg tcaaacagat tggtcgagga ggcatggcgg atgtctacct
    21481 agccaaagac ttaatcttag atggggaaga agtggcagtg aaggttctga ggaccaacta
    21541 ccagacggac ccgatagctg tagctcgttt tcagcgtgaa gcgagagcta tggcagatct
    21601 agaccatcct catatcgttc ggataacaga tattggtgag gaagacggtc aacagtatct
    21661 tgcaatggag tatgttgctg gactagacct caaacgctat atcaaggaac attatcctct
    21721 ttctaatgaa gaagcagtcc gtatcatggg acaaattctc ttggctatgc gcttggccca
    21781 tactcgagga attgttcaca gggacttgaa acctcaaaat atccttttga caccagatgg
    21841 gacggccaag gtcacagact ttgggattgc tgtagccttt gcagagacaa gtctgaccca
    21901 gactaactcg atgttgggct cagttcatta cttgtcacca gagcaggcgc gtggttcgaa
    21961 ggcgactgtg cagagtgata tctatgccat ggggattatt ttctatgaga tgttgacagg
    22021 ccatatccct tatgacgggg atagcgcggt gaccattgcc ctccagcatt tccagaaacc
    22081 cctgccgtcc gttattgcag aaaatccatc tgtacctcag gctttagaaa atgttattat
    22141 caaggcaact gctaaaaagt tgaccaatcg ctaccgctcg gtttcagaga tgtatgtgga
    22201 cttgtctagt agcttgtcct acaatcgtag aaatgaaagt aagttaatct ttgatgaaac
    22261 gagcaaggca gataccaaga ccttgccgaa ggtttctcag agtaccttga catctattcc
    22321 taaggttcaa gcgcagacag aacacaaatc aatcaaaaac ccaagccagg ctgtgacaga
    22381 ggaaacttac caaccacaag caccgaaaaa acatagattt aagatgcgtt acctgatttt
    22441 gttggccagc cttgtattgg tggcagcttc tcttatttgg atactatcca gaactcctgc
    22501 aaccattgcc attccagatg tggcaggtca gacagttgca gaggccaagg caacgctcaa
    22561 aaaagccaat tttgagattg gtgaggagaa gacagaggct agtgaaaagg tggaagaagg
    22621 gcggattatc cgtacagatc ctggcgctgg aactggtcga aaagaaggaa cgaaaatcaa
    22681 tttggttgtc tcatcaggca aacaatcctt ccaaattagt aattatgtcg gccggaaatc
    22741 ttctgatgtt atcgcggaat taaaagagaa aaaagttcca gataatttga ttaaaattga
    22801 ggaagaagag tcgaatgaga gtgaggctgg aacggtcctg aagcaaagtc taccagaagg
    22861 tacgacctat gacttgagca aggcaactca aattgttttg acagtagcta aaaaagctac
    22921 gacgattcaa ttagggaact atattggacg gaactctaca gaagtaatct cagaactcaa
    22981 gcagaagaag gttcctgaga atttgattaa gatagaggaa gaagagtcca gcgaaagcga
    23041 accaggaacg attatgaaac aaagtccagg tgccggaacg acttatgatg tgagtaaacc
    23101 tactcaaatt gtcttgacag tagctaaaaa agttacaagt gttgccatgc cgagttacat
    23161 tggttccagc ttggagttta ctaagaacaa tttgattcaa attgttggga ttaaggaagc
    23221 taatatagaa gttgtagaag tgacgacagc gcctgcaggt agtgtagaag gcatggttgt
    23281 tgaacaaagt cctagagcag gtgaaaaggt agacctaaat aagactagag tcaagatttc
    23341 aatctacaaa cctaaaacaa cttcagctac tccttaaaag cagatggata atcaaaatga
    23401 gtaatcaatc tttgtcatga tttcatggca aagatttttt ttgagtccag atttgtgata
    23461 gaatagaggg agttgataaa aggaggaaag catggaagaa tcaaaagaat taaatgccgt
    23521 cattgatgtg attatgctag cggggactat tctccttaaa agtggctcag aaatccatcg
    23581 tgtagaagat accatgattc gaatcgcgca ttcgcagggg attgtggatt gcaatgtcct
    23641 tgccatgcct gccgctatct ttttctctat tgaaaatacc aatatttcgc gcatgaagcg
    23701 cgtgacctcc tcttcttata acatcgaaaa agtctgcgat gtgaaccaga tttctcgtca
    23761 gctagttggg gggcagattg acttagaaac agcctttaag caattgacgg ccttgcaagc
    23821 tcaacccctt ccctatacta agttgcaggt aactctggct gcgaccttta gtgctccttt
    23881 cttttcagtt atgtttagcg gaaatatcta cgacgcactt ggtgcaggag tggcgacctt
    23941 atttggtttt gccttttccc tctatgtgga aaaatttatc cgaattccct ttgtgacagc
    24001 ctttgctgga gcctttgtct ttgggataat tgcccagttt tgggctcgct acacaggttt
    24061 tccttcaacg gcagatttga ttatagctgg tgcggtcatg ccgtttgtac caggtattgc
    24121 cttgaccaat gcggtccgtg atattatgac caaccacata aactctggta tgagtaagat
    24181 gtttgaatcc ctgctcatta ccctcgcttt aggggcagga acttctgtcg ccttggtatt
    24241 gatgaactaa tatgacacta acaacctttt tattacaagc agtagcaagt tttcttgcca
    24301 ttatcacttt tttaatcgta ctcaatgtgc aacggtctat gctcttacct ggagggattt
    24361 tgggcatgac tgtctggcta atctatctct tgctcaagga accgaccaat gtcattgtag
    24421 ctaccttcat tgcagccatt attggttctt gtgtcagcca gattttaagt attctttata
    24481 agacacctgc tgtggtcttt atcttggcca ttttggcacc gctggttcca ggttatctct
    24541 cctaccgaac aactgccttt tttgtgacag gggactataa taaagcactg gcaagtgcga
    24601 ccttggttgt catgttggct ttggtaatct ctattggaat ggctagcgga acagtgattc
    24661 tcagactgta tcattatata aaaacacatc gagtatcgta gactttacag aaataaaaga
    24721 attttctgaa aaatgagata aataaattaa caacgctttc tatatgtgcg agaataccgc
    24781 acttatgaag aaattgcgac tgattttggt atccacgaaa gcaacttact ccgtcggagc
    24841 caatgggttg aagtaactct tgttcaaagg tggtgttacg atttcaagaa ctcctctcag
    24901 ttctgaagac acggtaatga ttgatgcgac ggaagtaaaa atcaatcgcc ctaaaaaaaa
    24961 caattagcga atgattctgg taaaaagaaa tttcacgcta tgaaggctca agcgattgtc
    25021 acaagtcaag ggagaattgt ttctttggat atcactgtga actattgtca tgatatgaag
    25081 ttgttcaaaa tgagtcgcag aaatatcgga caagctggta aaatcttggc tgacagtggt
    25141 tatcaagggc tcatgaagat atatcctcaa gcacaaactc cacgtaaatc cagcaaactc
    25201 aagctgctaa cagttgaaga taaagcctat aaccatgcgc tatctaagga gagaagcaag
    25261 gttgagaaca tctttgccaa agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat
    25321 catcgtaaac gcttcggatt acgaatgaat ttgattgctg gcattatcaa tcatgaacta
    25381 ggattctagt tttgcaggaa gtctactact ttccttattg tctgtaaatc tactgacctt
    25441 gttgtttatc ccagtcatgg tttctagttc gggctcagag tttcaaagtg gatggcaaga
    25501 gcatcaattg attgctgaga aggttagtaa aacacttgat aagacatttg ataaggatgt
    25561 cagagaaatt ccgaccagtc agttttatca aaaatttgta gatgagatgg gaaggactta
    25621 ctcaggaaat ttgatcctcc aggagctgat aactgtgaat ggagcttata aagctactta
    25681 tatcggtgaa ctctctagca actaatcatt ctttaaaatg atgaccaata agaggcttga
    25741 tttggtgaat caagtctttt ttctctcttt tactgccatt tgtgataaaa tagtatagaa
    25801 cgatttttta catgaatgat aaaacagagg taaatatgac aatcggtatt gataagattg
    25861 gttttgcgac cagtcaatat gtcttgaaat tacaagactt agcagaagcg aggggaattg
    25921 accctgaaaa attaagtaaa ggactcttac tcaaggaatt gagtattgcg cccctaactg
    25981 aggacatcgt gaccttggcg gccagtgcta gtgactctat tttaactgag caagaaagac
    26041 aagaagttga catggtcatt gtggcgaccg agtcaggaat tgaccagagt aaggctgcgg
    26101 ccgtctttgt gcatggcttg ctgggcatcc agccctttgc tcgtagtttc gagattaaag
    26161 aagcctgcta tggggcgact gctgccctcc attatgccaa attgcatgtg gaaaattctc
    26221 cggagtccaa ggtcttggtc attgcaagtg atattgccaa atacggtatt gaaactccgg
    26281 gagaacccac tcagggtgcc ggaagtgtgg ctatgttgat tacacaaaat ccacgcatga
    26341 tggcctttaa taatgacaat gtagctcaga cccgtgacat catggatttc tggcgaccaa
    26401 attactcgac aactccttat gtaaatggtg tctattctac ccaacaatac ttggatagtt
    26461 tgaaaacgac ttggcttgaa tatcaaaaac gctaccagct tactttggat gattttgcgg
    26521 ctgtttgttt ccacttgcct tatcctaaat tagcgctaaa aggcttgaaa aaaatcatgg
    26581 ataagagcct gcctcaagag aaaaaagacc tcttacaaaa gcattttgac cagtctattc
    26641 tctacagtca aaaggtgggg aatatctaca caggttcact tttccttgga cttttgtctc
    26701 tcttggaaaa tacagatagc ttgaaagctg gggataaaat cgccctttat agttacggaa
    26761 gtggagctgt ggctgagttc ttcagtggtg aattggttga aggatatgaa gcttatttgg
    26821 ataaagaccg cttgaacaag ctcaaccaac gaactgtctt atccgttgca gactatgaaa
    26881 aggtcttttt tgaggaagta aacttggatg aaacaaactc tgcccagttt gctggctatg
    26941 aaaatcaaga ttttgccttg gttgaaattc tcgaccacca acgccgttat agcaaggttg
    27001 aaaaataatg aagataagtt ggaatgggtt ttctaaaaaa tcataccaag agcgcctcga
    27061 gctgttaaaa gctcaggcgc tccttagtcc tgagagacaa gctagtctgg agaaggatga
    27121 acagatgagt gtgactgtgg cagaccagct gagtgagaat gtagtgggaa ctttttctct
    27181 gccttattcg ctggttccgg aggtacttgt caacggtcag gaatacaccg ttccctatgt
    27241 gacagaagaa ccctctgtgg ttgcggcggc cagctatgcc agcaaaatca tcaagcgtgc
    27301 aggtggtttt actgcacaag tccatcagcg ccagatgatt gggcaggtag ccctttatca
    27361 agttgctaat cctaaactag cgcaagagaa gattgccagc aagaaagcgg agctcttgga
    27421 gcttgccaat caagcctatc cttctatcgt taaacgtggg ggtggggcgc gtgacctgca
    27481 tgtcgagcag ataaaaggcg aaccagactt tctcgttgtt tatattcatg tcgataccca
    27541 ggaagccatg ggtgccaata tgctcaacac catgctggaa gccttgaaac cagtcttaga
    27601 agaactcagt cagggacaga gtctcatggg aatcctgtcc aactacgcga ccgattctct
    27661 ggtgactgca agctgtcgca tggcctttcg ctacttgagc cgccaaaagg atcaaggacg
    27721 agagattgcg gagaaaattg cgttggctag tcagtttgcg caggatgatc cttaccgagc
    27781 tgctactcat aataaaggaa tttttaatgg tattgatgcg attttgattg ccactggtaa
    27841 tgactggcgt gccatcgaag ctggggccca tgcctttgcc agtcgagatg gacgctatca
    27901 aggtcttagt tgctggacgc tggaccttga aagagaagaa ttggtcggtg agatgaccct
    27961 gcccatgcct gtagcgacta agggtggctc tatcggcctc aacccacgtg tagctctcag
    28021 tcatgatcta ctaggaaatc cttctgccag agaattagcc cagattatcg tgtctatcgg
    28081 tcttgcccaa aattttgcag ccctcaaagc cttggtaagt acgggcatcc agcaaggcca
    28141 catgaaacta caggccaaat ccctagctct cctagctggg gctagtgaat ctgaagttgc
    28201 tcccctagtc gagcagctta tcgcagataa aacctttaac ctagagacag cccagcgcta
    28261 tctcgaaaat ttaagatcat aaaaaactca gacgaatcgg tctgagtttt cttgtgttta
    28321 aatacttttt cctggtaata gtgtaactgg taagaagtaa ccagttgtgg tgacttcctt
    28381 gccttcaatc ttttgcaaga gaagatcaat agtgagacaa gcaatctctt ccaaaggttg
    28441 cttgatagta gccaattgag ggtagtaatt ttcgataaag taggtcccat catagccgat
    28501 gaccttgagc tcttttggga cagaaatgcc caattcttga gcgattttaa tgaccagaat
    28561 agctgtcaaa tcatccgaag caaaaatggc atctggtttt tcccgggtca agatattctt
    28621 gatttccatt tcttttctga cgggagaaaa gtcactggaa acattgataa taggagcttt
    28681 tgggagtacg gatgcaaaac cagcgtggcg cagtccggtt ggcgaattgg aattgtcatt
    28741 ccctgtaatc atgatgatag actgggcacc tgtcttgacc aaggtttggg cagcaagaac
    28801 cccaccagca tagttgtcag aggagacgac agggatgtct ggcgataggt ttcggtcaaa
    28861 ggaaataatc ggcgctgtca cacgattgta gtcttcgatt cctaggttgt gactaccaga
    28921 aatgatgccg tccacctgat tggcttccaa catttcgatg tattcacgtt ccttctcaga
    28981 atcatgttca ctgttgcaga tgatggtctt gtaaccattt ttgaagagtt ggtgttccaa
    29041 tttatcaatc aattctgcat agaaaacatt ggaaatattg gggaaaatca agccgattaa
    29101 cttagctgat tttccttgca gactacgagc caggttgttg ggtttatagc ccaattctcg
    29161 catggcttca ttgacttttt ggatggtttt ctcagataga tacccttttt tattgataac
    29221 ccgagaaacg gtagtaggac tgacgcctgc aagtttggcg acatcagtta gttttgcgac
    29281 cataatctaa ttcatagtaa gttccagttg ggtttccaga tttgatcagg ataccatttt
    29341 ggtccgcatg tgggaagaca cgaccagaaa atactttttc tcctttattg atgaaaattt
    29401 caaagacaga gttatcgatg aagattgtag cagtagtagc ctgattctcg atagggcaag
    29461 aacgagttgt cccaaattct tgggcatact gttctccagc ctggctacga tccactgtca
    29521 cttgaccgtt tacaaggtca aagttgattg aaagtccctt accttcttta tcagcaagta
    29581 agacaatctc gctctggcta ttagcttcca agttgagttc aagttcgtaa gtgttcttgg
    29641 tttgggaacg gtttgagaag gcttcttcag aagcacgaag gtccttaata gcagcgactg
    29701 gatactggta gagcttgtcg tctttgatag tgagttcctt gaccaaagag aaggttcctt
    29761 ggtggtcaaa acggtcagat gggtaagaaa catctggcaa accaagccag ctaactgcta
    29821 gagcacgccc atcaggagcg ttgaaggctt gagttgcata ggcttcgaaa ccgtaatcca
    29881 tgttttgaag ttgagacaca tctaccattt tggcattttt agggtcaaag gaagccccaa
    29941 tcttatacat atttggaaag atattatcgt agtctagaac tttcttatcc aatccttgtg
    30001 gacagtagag aaggacaggt tgttcctcta caaagaccag attaggacat tccatcatgt
    30061 aggcagtacg gtcgttagca aagtcaaggt cgccaactgc ttgccagttt gtgtagtcgt
    30121 tattgacagc cttgtagaga cgaacgaaac cttttttctc caagtcctgt ccaccgacga
    30181 cagcatagta ttgaccttta aagttaaaaa tttgtggatc gcggaagtgg tcagtagagt
    30241 ctgctggctg gtcaatcaag atcttgtcaa tctttgtaat cttaccctcc ttgtccatca
    30301 aagcaccgat ctggtatggg tgacggatcc agttttcatc gcgaacattt cctgtataaa
    30361 ataggaataa gttatcgcca aattgcatgg cagaaccaga gtaggcaccg tggctatcta
    30421 atggagtatc tggtaaaact ttgattccag tttctttaaa gtgaaccaaa tcatcacttt
    30481 ctagctgtgc ccaagatttt aaaccgtggg ctgcaccaaa aggaaaattc tggtaaaaga
    30541 ggatccactt gccatcaaag taagaaaagc catttgggtc gttgagaagt cctgtttttg
    30601 gctcaacatg gtaatgagta tgccatggag attgtgccat attttccttt atatgttgaa
    30661 tttcttcttg cgtccaatct tgataaagtc tgtaacgacg ctcagttgtc cattccattc
    30721 attcctccaa aaatcttatc acttttaata gtaactgttt tcgataaaaa atgcaagcct
    30781 tttatgttaa aaaagaggaa aaactgtcaa aggattatca taaaataaat acaggaaatc
    30841 aatcaaattt ttacgaaaat gtgaaagata atgaaataaa agacttttta accaaggttt
    30901 caaacttatt tttcaaggaa ttcctgatga aacagtcaaa aacaatcaat ctaaagcttg
    30961 cccactaagt ggatagaata tttttctgca aaacgcttga catattttta aaacatgata
    31021 aaataatagt cgtaagggcg aataaaatcg ctttcaaaca agaacaaaat gttatataag
    31081 gagatttttg caaatgaaca atcaggaaat tgcaaaaaaa gtcatcgatg ccttgggcgg
    31141 acgtgaaaat gtcaatagtg ttgcccactg tgcgactcgt ctacgtgtca tggtcaaaga
    31201 tgaagagaaa atcaataaag aagtgattga gaacttggaa aaagttcaag gtgctttctt
    31261 taactcaggg caataccaaa ttatctttgg tacaggtaca gttaacaaaa tgtacgatga
    31321 agttgttgta cttggcttac caacatcatc taaggatgac atgaaagcag aagttgctaa
    31381 acaagggaac tggttccaac gtgctatccg tacttttggt gatgttttcg ttccaatcat
    31441 cccagttatc gtagcgacag gtctcttcat gggtgtgcgt ggtcttttca acgctcttga
    31501 aatgccactt ccaggtgact ttgcaactta cacacaaatc ttgacagata cagccttcat
    31561 catcttgcca ggtttggttg tgtggtcaac cttccgtgta tttggtggaa atcctgccgt
    31621 tggtatcgtt cttggtatga tgcttgtctc tggctcactt ccaaacgctt gggcagttgc
    31681 tcaaggtggt gaagtaacag cgatgaactt ctttggtttc atccctgttg ttggtttgca
    31741 aggttccgtt cttccagcct tcatcatcgg ggttgtcgga gctaaatttg aaaaagctgt
    31801 ccgcaaggtt gttccagatg tcattgacct cttggtaaca ccattcgtga cacttttggt
    31861 catgtctatc cttggactct ttatcattgg accagttttc cacgttgttg aaaactacat
    31921 ccttattgct acaaaagcga ttcttagcat gccatttggc cttggtggtt tcttgattgg
    31981 tggggttcac caattgatcg tcgtgtcagg tgtgcaccac atcttcaact tgcttgaagt
    32041 gcaattactt gctgctgacc atgctaaccc attcaacgct atcatcacag ctgctatgac
    32101 agctcaaggt gctgctactg ttgcggttgg tgttaaaaca aaaaatccaa aactgaaaac
    32161 acttgctttc ccggctgctc tttctgcctt cctaggtatt acagagcctg ctatcttcgg
    32221 ggtgaacttg cgcttccgta aaccattctt cctttcattg attgctggtg caatcggtgg
    32281 tggattggct tctatccttg gacttgctgg tactggtaat ggtatcacca tcatccctgg
    32341 tacaatgctt tatgttggta acggacaact tccacaatac cttcttatgg tagctgtatc
    32401 atttgccctt ggttttgctc ttacttacat gtttggttac gaagatgaag tagacgcaac
    32461 tgcagctgca aaacaagctg aagtggctga agaaaaagaa gaagttgcgc cagcagctct
    32521 tcaaaatgaa acacttgtaa ctcctatcgt cggtgatgtt gtcgctcttg ctgatgtcaa
    32581 tgacccagtc ttctcaagtg gagctatggg acaaggtatc gctgtgaaac caagccaagg
    32641 cgtggtctat gcaccagctg atgctgaagt ttcaattgcc tttccaacag ggcacgcttt
    32701 tggtttgaaa acaagaaatg gtgctgaagt tttgattcat gttggtattg atactgtatc
    32761 tatgaacggt gacggttttg aaacaaaagt tgctcaaggt gataaggtga aagttggcga
    32821 tgttcttgga acatttgact caaacaaaat cgctgcagct ggacttgatg atacaacaat
    32881 ggttatcgtt acaaatacag gtgactacgc ttcagtagct ccagtcgcaa caggttcagt
    32941 tgctaagggg gatgctgtga tcgaagtgaa aatctaatca atcctctcta atgtgaaaac
    33001 gaacaaatga catatttgtt cgtttttatt agatagtaag atttacagag gaaaatctaa
    33061 aaaatagaga catttagact ttcgaagtat gctataataa agaaaataaa aacaagaggt
    33121 ttatcatgac aaaattatat ggaagcttgg aagcgggcgg tacaaagttt gtctgtgctg
    33181 tcggtgatga aaactttaac gttgtagaaa aaacacaatt tccaacaaca actccaatcg
    33241 aaacaatcga taaaaccatt gagttcttct caaaattcga taaccttgct ggtcttgcag
    33301 ttggttcatt tgggccgatt gatattgaca aaaactcaaa aacttatggc tttatcacga
    33361 cgactccaaa accaaactgg gcaaatgtgg acttgcttgg tgcccttcgt cgcgccctaa
    33421 atgtgccaat gtacttcaca acagacgtaa acagctctgc ttacggtgaa gtggttgccc
    33481 gtaacaatgc tggtggtcgt atcgaaaact tggtttacta cacaatcggt acaggtatcg
    33541 gtgcaggtgt catccaacgt ggtgagttta tcggtggtgt gggtcaccct gaaatgggtc
    33601 attattatgt tgctagacac ccaatggata ttgaaaaaga gtttaagggt gtttgtcctt
    33661 tccataaggg atgtctagaa ggttatgcag ctggtccaag tttggaagct cgtacaggtg
    33721 tacgtgggga aactattgaa ctcaacaacc ctgtttggga tgttcaagcc tactatatcg
    33781 ctcaagctgc ggttaatgcg acagtgactt tccgcccaga cgtgattgtc tttggtggag
    33841 gggtcatggc tcaacaacat atgctggacc gtgtccgtga gaaatttaca tctcttctta
    33901 atggttacct accagtacca gatgtgcgtg actatatcgt gacgccagca gtcgcaggaa
    33961 atggttctgc cacacttggg aactttgttc ttgcaaaaga agtttcaaaa taaagcacaa
    34021 aatccgcttg ggaaaccaaa cggatttttt acgtgtcaaa gcatttgcca gaaaaagtca
    34081 ataatatagg tcagaccgta ggtataaacc acgagggtaa agggtttggt cagaatgatg
    34141 atggtcatgt agcgcttgaa actcatcttg gtcagggcag ccagcataca gagaaagtca
    34201 gctgggctaa tgggccaaat catcataaaa ataaagaagc ggtcaaaacg attgccctta
    34261 tctagccagc cgatgtactt gtcgtaggtg cgcttgctga cgacagactg gacaaaggca
    34321 gctccgtata ggcgcactag ataaaagata atggcacagc caatcacgat gccgatatag
    34381 ttgtagatag tcccgatgat gtgcccgtag ataaagaccc cagccaccga ggtcaaggcc
    34441 cctggaatga tagggacgac agtctgtaaa atctgtaaaa agataaagag aggtggaccc
    34501 cagatgcctg cctgctggat aaaggcagag agggtttcct tggattgtaa aatcccagcc
    34561 tgataagccc aaatacagaa aatcaaactc ccaaccccac cgacaatcga tgagatattg
    34621 ataactcgct gcagaagggg agaaacagct ttcatttgtt tatcctgtga catgtaaact
    34681 cctcatagat agtatatttc tactatacca tattttggag gagaaagggg gaatgagtct
    34741 tttttgataa ggaatagaaa gtaaatagcc cttcgagact tagaataggt ctgtcaacta
    34801 aaggttatat gcattctagg tttcgaataa tcgcaattcc ataaatttaa aagtattcta
    34861 agtttcgaat agcttatctg ggataatttt gaggcattcg cgctttcgaa tagtcatttt
    34921 ccctaaagtt tatatgtatt ctgaagctag aaagactaaa atataaaagt agtaagagtc
    34981 tctctctgat ggacgtaatt tatggctagt atgaatcctt tgaattgtta gttaatttta
    35041 atattaatca gttctgaaat aattactaaa tatagttaga tggtgagtta attattgcta
    35101 aatttcaagt tcaagttagt catccagaag tcttctctgg gggacttgta gttcaagcat
    35161 tttttaggat agttgttaat ccacttttcg ataaatgcga cttcctttgt agttgttttc
    35221 ttagttccct gaggtaacca tctatgaatg agcctgttgt gattttcatt agatcctata
    35281 gtgatgtgca taatagatat agcagattga aactagaata gtatacatct acttctaaaa
    35341 cattgttaga aatcaatttg actgtcctga tcaatttgtc ctgtttttat ttcattttac
    35401 tatataatcc ttagaaaaca tatcggacaa gcggttgaat tccgttccgt ccattagtag
    35461 ataaaggatt actccttctt gaaatgacag atgaacttgt ttcttataaa aatgttgtct
    35521 gaaactatta gcctttttcc agaagtttta gaggaagaaa actttacgag gaaaaaggag
    35581 ttactcaaat aacaattcag gattaaaaat agacagttga ggagcggaag gtataaatta
    35641 agtttgctac tgtataatgg atttatcact aatactcttc gaaaatctct tcaaaccacg
    35701 tcagcttcgc cttgccgtag atatagttac tgacttcgtc agttctatcc acaacctcaa
    35761 aacagtgttt tgagctgact tcgtcagttt tatctgcaac ctcaaagctg tactttgagc
    35821 agcctacggc tagcttccta gtttgctctt tgcttttcat tgagtataaa agtgctccag
    35881 gccatatttt acacagcaat agtcttctaa aaatggacaa aaacattgta aaggctatca
    35941 aaaaggagta taatgagtgc aagaaatttc ggaggtgaaa gatgctagaa gtaagaagtc
    36001 tagagaaaag ttttggctcc aagcaagttt tgtttggtat tgactttcaa gcgcgaccag
    36061 gtcgtatttt gggactagtc ggaaaaaatg gtgctggaaa aacaacgatt ttccatagta
    36121 ttttgaaatt cctagaatat cagggagaaa tcggtctgga tggtcaggat attcgtcagg
    36181 agacctatgc tcggattggt tatctgcctg aagaacgcag tctcatgcct aaattgacag
    36241 ttcttgaaca agttcgctac ttggcgactc taaaaggcat ggatgccaaa gaagtcaaag
    36301 aaaaactccc tcaatggatg aagaggttgg aagtgaaagg aaagctgaca gataaaatca
    36361 agagtctgtc aaaaggaaat cagcagaaga ttcagctcat tattactctg attcatgaac
    36421 cagacctgat tatcttggat gagcctttta gtggattgga cccagttaat acagaattgc
    36481 tcaaacaagt catttttcag gaaaaagagc gcggagcaac cattatcttt tctgaccatg
    36541 tcatgaccaa tgttgaggaa ctttgtgacg atattctgat gatccgagat ggccgtgtgg
    36601 tcttgcacgg accagttcag gatgtccgca atcaatacgg gaaaacgcgt ctctttgttt
    36661 caagtgaacg aagcaaggaa gaattggaaa atcttcctca tgtcaaacag gtgagcttga
    36721 ccaaacaagg cagttggaaa ttgattttgg aggatgagag cgctggaagg gaactcttcc
    36781 caatcttgac tcaaggtcaa tatatcgcaa catttgacca acaagcgcca actatcgatg
    36841 aaatctttaa actagaatca ggagtggaag tatgagaaat atgtgggttg taatcaagga
    36901 aacctatctt cgacatgtcg agtcatggag tttcttcttt atggtgattt cgccgttcct
    36961 ctttttagga atctctgtag gaattgggca tctccaaggt tcttctatgg ctaaaaataa
    37021 taaagtggca gtagtgacaa cagtgccatc tgtagcagaa ggactgaaga atgtaaatgg
    37081 tgttaacttc gactataaag acgaagcaag tgccaaagaa gcaattaaag aagaaaaatt
    37141 aaaaggttat ttgaccattg atcaagaaga tagtgttcta aaggcagttt atcatggcga
    37201 aacatcgctt gaaaatggaa ttaaatttga ggttacaggt acactcaatg aactgcaaaa
    37261 tcagcttaat cgttcaactg cttccttgtc tcaagagcag gaaaaacgct tagagcagac
    37321 aattcaattc acagaaaaga ttgatgaagc caaggaaaat aaaaagttta ttcaaacaat
    37381 tgcagcaggt gccttaggat tctttcttta tatgattctg attacctatg cgggtgtaac
    37441 agctcaggaa gttgccagtg aaaaaggcac caaaattatg gaagtcgttt tttctagcat
    37501 aagggcaagt cactatttct atgcgcggat gatggctctg tttctagtga ttttaacgca
    37561 tattgggatc tatgttgtag gtggtctggc tgccgttttg ctctttaaag atttgccatt
    37621 cttggctcag tctggtattt tggatcactt gggagatgct atctcactga ataccttgct
    37681 ctttattttg atcagtcttt tcatgtacgt agtcttggca gcattcctag gatctatggt
    37741 ttctcgtcct gaggactcag ggaaagcctt gtcgcctttg atgattttga ttatgggtgg
    37801 tttttttgga gtgacagctc taggtgcagc tggtgacaat ctcctcttga agattggttc
    37861 ttatattccc tttatttcga ccttctttat gccgtttcga acgattaatg gctatgcggg
    37921 gggagcagaa gcatggattt cacttgctat tacagtgatt tttgcggtgg tagcaacagg
    37981 atttatcgga cgcatgtatg ctagtctcgt tcttcaaacg gatgatttag ggatttggaa
    38041 aacctttaaa cgtgccttat cttataaata gaagagcctt gcgaaagcga ggtttttaaa
    38101 gatgaaaagg atggaatcac gaacctaatt atgtacacat tatgtaaaca ggttgcttga
    38161 aaactcaatt gtatagtgaa ctgaatctgg aataggataa tattacttct aaaacattct
    38221 tataaattga tttgaattcc aaaatcccat cgttccgttc ttatttcatt ttgcgatata
    38281 gtgaaaggaa ataagtcagc tcacaaaaaa cgcataatat caaggatttt taacacttga
    38341 tactatgcgt tttctaattt taaagacttt ttgcctggtc tcttttgttc tatgagtatg
    38401 ttacatatct aggtaaaaat tatttaaagc aaattaacct tatttctcaa gatgtagtag
    38461 gttccgagat aaaagatagc tatgaatatg agttctttaa caatggctat acctgctccc
    38521 atatagaaat ggtcattgag tcctaccagt gaattgacat agaaattaga actaagattg
    38581 aagaaaagtt caataaatcc aatgacgatt tggataccaa tgtaggctgc aacagcgagt
    38641 gctgtacggt attcattgaa aagctgtcca atggaaatag ccaggtagat gcagaggatt
    38701 cctgaaatgg tatttagtag gaaggatata cctgtaagaa agatctgagg gagatgtgtt
    38761 tctacaaatg taatcacata agaaagagga atccattctg gagctgttaa agccagaata
    38821 ataacagcac ttagagccaa tacagcggtg ctaatcaatg accagataaa ggcaccgatt
    38881 agtttggctg tgatgatatg gtgttcagaa actggcaagg tcaaagtcag atagccttgt
    38941 cggtcgtaga cactaccttt gaagcgttta ataatcaaga aaatggttga aatcgcaagt
    39001 gtaagcatca agccaccaaa gactgtagct agaaaaagta gcatagtagc ctgactttct
    39061 tggtaaggta gatttttaaa gccttgtgtc tgtattccga tgagggcaga aaggactagc
    39121 acggctgcgt agagggctaa ataccacttg ttaacatttt taaattcgta gtgaactaaa
    39181 ttccaaaaca taaataatct cctttgctta ggccttaaat tcctgacgga agagttggtc
    39241 aatggattca cctgactcgt agcgaatatc atctacattt ccttgacgga cgacttttcc
    39301 gtcttttagg aagacaattt catccaagat tggctcgata tcagaaatca agtgggtaga
    39361 aatcaaaacg gtagaagttg gtgagtagtt gttgataatg gtattgagga tataagcacg
    39421 ggctgctgga tccaccccac caatgggttc atccaaaaca tagagacgag catcacggct
    39481 cataaccaaa atcagttgaa ccttttcttt gtttcctttt gatagtttct tgagacgact
    39541 attttcatca atgcccaggt ctgcaagtag atgatgagcg cgttcaagat tgaaatcttt
    39601 atagaaggtc ttgaagtagg ttagggcttc tttgaccttc atttgctcat tgagataggt
    39661 cgtatcaggc aaataagcta caacggcctt ggttgctggg cttgggtcca tgtcgttgat
    39721 gaggacacgt ccttgatctg gttgtaagag gccattaatt agtttaatca gggttgtttt
    39781 tcctgagccg tttggcccaa gaaggccgac aatttttcca gctggaatgt caagagaaac
    39841 attttcaagg gctggtgttg ctccatatga tttggataca ttttcaaata ctagtaatga
    39901 cataggctta aactccttta atataatcac tgactacgcc tggtagttct tctttttcat
    39961 agccaaaatg gatcatggag gaaacgaagt gttccaattc ttcttctgat aattgtttgc
    40021 gtgactgagc gattagctcc ttatccttag tcacaaatcg tccagttgtt cgcttgctgt
    40081 agacaaatcc ttctcgttca aggtctgata aggctctttg gatggtattg ggattgacac
    40141 cagcctcgct agctagctcc ctcacggttg gaagttgttg attgggttcc agtgtatggg
    40201 aaacaatctg aagcttgatt ttctccataa tctgtaaata gatgggtttt ttgttgtcaa
    40261 atgtccagga catcttagtc tcctttctct tatccttttg tcttaactaa ataatacaac
    40321 aaaacaaaaa acttgtcaag caaaaagaag aattgttttg ggaatcgctt aaaaaatggt
    40381 ataatgaaaa gaaaacgata aggagggaaa ggatgcgttg tccaaaatgt ggggctacca
    40441 agtcaagtgt tatcgatagt cgccaagcag aagaagggaa caccattcgt agaagacgtg
    40501 agtgcgacga atgccaacac cgttttacaa cctacgaacg agtagaagaa agaaccttag
    40561 tggttgttaa aaaagatggt acacgggaac aattctccag agataaaatc tttaatggga
    40621 ttatccgctc agcccagaaa cgtcctgtgt caagtgatga aatcaacatg gtagtcaatc
    40681 gtatcgaaca gaaactccgt ggtcgaaatg aaaatgaaat tcaaagtgag gacattggtt
    40741 cactcgtcat ggaggagttg gctgaattgg acgagattac ctatgtacgt tttgctagtg
    40801 tctatcgtag ttttaaggat gtcagtgagt tagagagctt gctccaacaa atcacccagt
    40861 cctctaaaaa gaaaaaggaa agataaatga agccaattga ccgtttttct tatctaaaga
    40921 ataatcgggt gtcgcaagat acctcatctc tggtacagtg ctacctcccg attatcggtc
    40981 aggaggcact gagcctttat ctttatacaa tcagtttttg ggataatggt agaaaggaat
    41041 atcttttttc aagtatcctc aatcatctca actttgggat ggatagactg ataaaatcat
    41101 tgaaaatctt atctgctttt aatctcttga ccctctatca aaagggggat gtttatcagc
    41161 tagccctcca tgctcctcta tctagtcaag acttcttgga gcatcctgtt tatcgcagac
    41221 tcttagagaa aaagattggc gactctgctg tggaggattt gaaagttgaa agtgctgatg
    41281 gtgaagaaat acctgtctca ctcaatcaag tctttccaga attggcagaa ctaggcagtc
    41341 aagaagacct tggtctcaag aagaaagtgg ccaatgattt tgacttggaa cattttcgcc
    41401 agctgatggc tcgagatgga cttcgttttg cggatgagca gtccgatgtc ttaaacctct
    41461 ttgctattgc cgaggagaag aaatggactt ggtttgaaac ctatcaattg gccaagtcaa
    41521 cagctgtttc tcaggttatt tcaaccaaac gcatgcgtga aaaaattgct caaaaaccag
    41581 tttcctctga ctttaatcct aaggaagcaa ccattatcaa ggaagctaaa agtaagactg
    41641 ccctgcagtt cttagcagaa atcaagcaaa cacgcaaggg gaccattacc caaacagaaa
    41701 gagaactttt gcaacagatg gctggcttgg gcttgctgga cgaagtcatc aatatcattc
    41761 tcttgttgac ctttaataag gtggattcgg caaacatcaa tgagaaatat gccatgaagg
    41821 tggctaatga ctatgcctat caaaagatac attcagcaga agaggccgtt ttacgaattc
    41881 gtgagcgagg gcagaaaaca aaaacacaaa aacagaatca gactgcccca gcaaaaacca
    41941 atattcctaa atggagtaac ccagattata agaatgaaac cagcgaggaa actcgtctgg
    42001 aactagaacg taagaaacaa gaactattag ctcgattaga aaaaggagga gattagatgg
    42061 aaagtgtcgg agacgtactc aaacgtcaac ccagccgttt ttattatcaa gatttggtcc
    42121 agaaaatcat gaaggaccct gatgttgcgg cttttatcca gcaagaatcc ttgactccag
    42181 aggaattaaa tcgcagtatc tccaagttta atcagtacat caccgagcgt gacaagtttc
    42241 tccgtgggga tacggattat attgccaaag gctacaagcc gattttggtt aagaatcatg
    42301 gctatgcaga tgtttcatat gaagaaactc ctgagctaat cgcggctgaa aaagaagcgg
    42361 ctattaagaa tcgtctcaag ctaatcaatc taccagccag tctcaaaaaa gctagtttgg
    42421 ctcaagttga tttggatgat ttgggacgct tgccagtttt tgaaaaacta ttagcctttg
    42481 tggagcaata tccagctatt cgaaaaggtc tttacttata tggagacttt ggtgtgggta
    42541 aaagtttcat ggtggcggcc ttagctcatg atttatcaga aaaacgtggt gtttcatcaa
    42601 ctctcctcca ctatcctagc tttgtcattg atgtcaaaaa tgctatcagt gatggcaatg
    42661 ttaagacctt ggtggatgag attaagctgt ctgaggtcct gattttagat gatattggcg
    42721 ccgagcaatc aaccgcttgg gtgcgtgacg aaatcctgca ggtcattctc caatatcgga
    42781 tgcaggaaaa tttaccgacc tttttcacat ccaacttcaa ctttgaagat ttggagaagc
    42841 attttgcgaa agtgaaacat ggaaatgacg aaacctggga agccagacgg gtcatggaac
    42901 gcatccgtta tttggctgag gagactcgtt tagaaggagt aaaccgtcga tgacagaaac
    42961 cattaaattg atgaaggctc atacttcagt gcgcaggttt aaagagcaag aaattcccca
    43021 agtagactta aatgagattt tgacagcagc caagatggca tcatcttgga agaatttcca
    43081 atcctactct gtgattgtgg tacgaagtca agagaagaaa gatgccttgt atgaattggt
    43141 acctcaagaa gccattcgcc agtctgctgt tttccttctc tttgtcggag atttgaaccg
    43201 agcagaaaag ggagcccgac ttcataccga caccttccaa ccccaaggtg tggaaggtct
    43261 cttgattagt tcggtcgatg cagctcttgc tggacaaaac gccttgttgg cagctgaaag
    43321 cttgggctat ggtggtgtga ttatcggttt ggttcgatac aagtctgaag aagtggcaga
    43381 gctctttaac ctacctgact acacctattc tgtctttggg atggcactgg gtgtgccaaa
    43441 tcaacatcat gatatgaaac cgagactgcc actagagaat gttgtctttg aggaagaata
    43501 ccaagaacag tcaactgagg caatccaagc ttatgaccgt gttcaggctg actatgctgg
    43561 ggcgcgtgcg accacaagct ggagtcagcg cctagcagaa cagtttggtc aagctgaacc
    43621 aagctcaact agaaaaaatc ttgaacagaa gaaattattg tagaaagtga gaaattatgg
    43681 ccctaccaac tattgccatt gtaggacgtc ccaatgttgg gaaatcaacc ctatttaatc
    43741 ggatcgctgg tgagcgaatc tccattgtag aagatgtcga aggagtgaca cgtgaccgta
    43801 tttatgcaac gggtgagtgg ctcaatcgtt cttttagcat gattgataca ggaggaattg
    43861 atgatgtcga tgctcctttc atggaacaaa tcaagcacca ggcagaaatt gccatggaag
    43921 aagcagatgt tatcgtcttt gtcgtgtccg gtaaggaagg aattaccgat gcagacgaat
    43981 acgtagcccg taagctttat aagacccaca aaccagttat cctcgcagtc aacaaggtgg
    44041 acaaccctga gatgagaaat gatatctatg atttctatgc tctcggtttg ggtgaaccat
    44101 tgcctatctc atctgtccat ggaatcggta caggggatgt gctagatgcg atcgtagaaa
    44161 atcttccaaa tgaatatgag gaagaaaatc cagatgtcat taagtttagc ttgattggtc
    44221 gtcctaacgt tggaaaatca agcttgatca atgctatctt gggagaagac cgtgttattg
    44281 ctagtcctgt tgctggaaca actcgtgatg ccattgatac ccactttaca gatacagatg
    44341 gtcaagagtt taccatgatt gatacggctg gtatgcgtaa gtctggtaag gtttatgaaa
    44401 atactgagaa atactctgtt atgcgtgcca tgcgtgctat tgaccgttca gatgtggtct
    44461 tgatggtcat caatgcggaa gaaggcattc gtgagtacga caagcgtatc gcaggatttg
    44521 cccatgaagc tggtaaaggg atgattatcg tggtcaacaa gtgggatacg cttgaaaaag
    44581 ataaccacac tatgaaaaac tgggaagaag atatccgtga gcagttccaa tacctgcctt
    44641 acgcaccgat tatctttgta tcagctttaa ccaagcaacg tctccacaaa cttcctgaga
    44701 tgattaagca aatcagcgaa agtcaaaata cacgtattcc atcagctgtc ttgaacgatg
    44761 tcatcatgga tgccattgcc atcaacccaa caccgacaga caaaggaaaa cgtctcaaga
    44821 ttttctatgc gacccaagtg gcaaccaaac caccaacctt tgtcatcttt gtcaatgaag
    44881 aagaactcat gcacttttct tacctgcgtt tcttggaaaa tcaaatccgc aaggcctttg
    44941 tttttgaggg aacaccgatt catctcatcg caagaaaacg caaataaaaa agtagaatct
    45001 ggaatgacag ttccggattt ttttgatata ataaaataat agaaaacgct atcaaaagaa
    45061 ggagggtgat gtcggtgatg gaacatttgt ttaaattctt acttttagca ccgtactttt
    45121 attttgataa ctggattgag aaggccaaca gaaatagtaa gttttttcca attttttatt
    45181 atttttactg gttttacatc cccttctatt ctctttttag ccttgcttgg acagttgttt
    45241 cagttctgtt tttcaatacc gtcttgagaa atgtgacaga tatcaagtta tggggcattt
    45301 ggtttctttt tattctgcta gctattggta tgaattggtt aacttattcc tgtttcaaag
    45361 aaatgtttcg cttgagacag gaactaggga agtctaaggg tggaaggcat tgatttatat
    45421 cggtttctat ttgtaggagg tggcttatgg tacctctaaa atcatttatt acacgctatt
    45481 ccaaggttta tattggtaat aacatttaat agaacgaaac taaagaaaac aatttaaaag
    45541 aggaggtttg tgatggaata cttgtttaaa atgataatct tattaccctg tttttacttt
    45601 ttcagctgga ttgaaaaggc aaacagagat agtaagattt tcccaatttt ttactatttt
    45661 tactggattt acatcaccct ctatgctctt tttagccttg cttggacagt tttttcagtt
    45721 ctgtttttca atatcgtctt gagaaatttg acagatatca agttatgggg tatttggcta
    45781 ttattactac ttatcgcttt tgctagtgac tggttagact atatctgttt caaaaaaatg
    45841 cttgacttga gacgggaact agggaagtct aaaggcggaa ggcattgatt tatactcttc
    45901 gaaaatctct tcaaaccacg tcaacgtcgc cttggattat atatgtaact gacttcgtca
    45961 gtcttatcta caacctcaaa gcagtgcttt gagcaacttg cggctagttt cctagtttgc
    46021 tctttgattt tcattgagta ttatattact ttctatttgt aggaggtggc ttatgaagat
    46081 tcctctctta actttggcaa ggcataaatt tgtttatgtc ttgcttactt tgctttttct
    46141 tgctttggtt tatcgtgatg ttttgatgac ttatttcttt tttgatattc atgcgcccga
    46201 tctagctaaa ttcgatggac aagcaattaa aaatgactta ttaaaatcag cattagattt
    46261 tcgtattctc cagttcaatc taggttttta tcaatcattt attattccaa tcatcattgt
    46321 tttgctaggt tttcaatata ttgagctgaa aaataaagtt ttacgattga gtattggaag
    46381 agaagtgagc tatcaagggt taaaaagaaa gttgactttg caagttgcaa gtatcccttg
    46441 tttgatatat ttagtgactg tgctgataat tgcaattata acctatttct ttgggacttt
    46501 ttctcctctt gaatggaatt ctctattttc tgatgggagt ggtttacaaa gactcctaga
    46561 tggagagata aaaagctatt tgttctttac ttgtgtccta ctaatcggta ttttcatcaa
    46621 tgcaatctat tttttacaaa tagttgatta tgtggggaat gtgactcgtt cggcaatcac
    46681 ctatttgatg tttctttggc ttggttctat gctgctctat agtgccttgc cttactatat
    46741 ggttcctatg acgagtttga tgcaagctag ctatggggat gtaagtttga tgaaactctt
    46801 tactccttat atccttcatg ttgtccctta tatggtactt aaaaaatatg aagataatgt
    46861 ttaagaattt taacaatatt tggctaaaga gaaagatcgt tttactactt cgtatagttc
    46921 tgatgatgat tttgataaac tatctattgt caacagcggt tcaaaagcag gatgctgtta
    46981 tctttttcaa gagagaattg atttcaattt tttcctataa tgactattct gaagcgaatt
    47041 tagaaatccc caaactcttg ttaaaccttt cgcttttcat ggtaggatgg ctctctgtca
    47101 ttttacttga aagtgatttg gcagaccatt accatcactt gattcgctat caatcaagct
    47161 cctttttcga ttatacaagg aaacgattgg ttgtcatttc taaatttttt actcaagatt
    47221 tgtttgtctg gtttcttggt ttacttccgc taggaattca tttcaaaaca gtcgcacttt
    47281 tctttttact tgctcagtta atgatgttgt acttactact gtcttatctg atagcactga
    47341 ttagtgcggg cgctggtttt tctttttttc tctatttttt ggcatttgtg ggacaagaat
    47401 ggatgatgga tcatattgta acagtgtatt tagtactctt aagcttatta gttatgttga
    47461 ttgttagtcg cttggaagag aaatttaaga aaggataaac gatgagactt gaaattataa
    47521 atggacagaa aatttatggg aaaagaccta ttttaaatca gttgaatttg gtgtttcaat
    47581 caggaaaaat ttatggactt aaaggtgata atggatctgg caagacggtt cttttaaaga
    47641 tacttgctgg ttatattaag cttgacaaag gaaaagttct tcaagatggt aaagtttacg
    47701 gggtaaaaaa tcattatatt caggatgcag gaattttaat tgaaaaagtc gagtttttat
    47761 ctcatttatc cctgagagaa aatttggaac tgttaaggta tttttcatct aaagttacgg
    47821 aaaaaagaat tgcctattgg attcaatact atgatttaca ggaatttgaa gacgttgaat
    47881 accgtcattt atccttggga acaaagcaaa aaatggcctt gattcaagcc tttatttcct
    47941 ctccttctat actctttctc gatgaaccta tgaatgcttt ggatgagaag agtgtgaggt
    48001 taaccaaaca ggtcatttta tcttacctga aaaaagaaaa tggtctggtt atcctgacgt
    48061 cgcacatatc ggaagatatt tcagaccttt gtacagatgt attagttgtc gaaaatggac
    48121 atatacaata tgtaaaggat atacaatcct aggagatggc ttatggcaca tctaaaatca
    48181 tttattacac gctattccaa ggtttatatt ggtttagttc tgctgatctg gctgtctttc
    48241 ttctttatcc cttgggatag agcgattctg ggggtaagga ttgacatctt catcatgcag
    48301 aaaatcttgc tagcttttgg aattctgtcc attctcatgg ccttgctgtc caagaaagtc
    48361 agtctctttg tttttggact gatttgctgt ctttctcttt ggattaactt atttatcaca
    48421 tttgccattt tgccgatttt tggcaattaa acagtcataa aagtcggaga ggttagcttg
    48481 aaaactaacc tctttttcct tttcaaaatg gggattcttc cttgaaaata atcagtaatt
    48541 gtgctaaaat taaaggaaca ttctaaaata ttcggaattt aaagtaagga aaaacatggc
    48601 taatatttta aaaacaatta tcgaaaatga taaaggagaa atccgtcgtc tggaaaagat
    48661 ggctgacaag gttttcaaat acgaagacca aatggctact ttgactgacg accaactaaa
    48721 agcaaaaaca gttgaattta aggaacgtta tcaaaatgga gaatcactgg attcattgct
    48781 ttacgaagca tttgcggttg tccgtgaagg tgccaaacgt gtcctaggtc tcttccctta
    48841 taaggttcag gtcatggggg ggattgttct tcaccatggt gacgtgccag agatgcgtac
    48901 aggggaaggg aaaaccttga ctgcgaccat gccggtatac ctcaatgccc tttcaggtaa
    48961 aggggttcac gtagttacgg ttaatgaata cctgtcagaa cgtgacgcga ctgagatggg
    49021 tgaattgtac tcttggcttg gtttgtcagt agggattaac ttggctacca aatctccaat
    49081 ggagaaaaaa gaagcctatg agtgtgatat tacttactca actaactcag aaatcggatt
    49141 tgactacctt cgtgacaaca tggtcgttcg cgccgaaaac atggtacaac gtccgcttaa
    49201 ctatgccttg gtcgatgagg ttgactctat cttgattgac gaggctcgta cacctttgat
    49261 tgtatcaggt gccaatgcgg ttgaaaccag tcagttgtat cacatggcag accactatgt
    49321 aaaatctttg aacaaagatg actacatcat cgatgtgcag tctaagacta ttggtttgtc
    49381 tgattcaggg attgacaggg ctgaaagcta cttcaaactt gaaaacctct atgacatcga
    49441 aaacgtggct ttgactcact ttatcgataa cgcccttcgt gccaactaca tcatgcttct
    49501 cgatattgac tatgtggtga gcgaagagca agaaatcttg attgtcgacc aatttacagg
    49561 tcgtaccatg gaaggtcgtc gttattctga tggattgcac caagctattg aagccaaaga
    49621 aggtgtgcca atccaggatg aaaccaagac atctgcctca atcacgtacc aaaacctttt
    49681 ccgtatgtac aagaaattgt ctggtatgac gggtacaggt aagactgagg aagaagaatt
    49741 ccgtgaaatc tacaacattc gtgttattcc aatcccaaca aaccgtcctg ttcaacgtat
    49801 tgaccactca gaccttcttt atgcaagtat cgaatctaag tttaaagcgg ttgtcgaaga
    49861 cgttaaggct cgttaccaaa agggtcaacc tgtcttggtt ggtacagtgg cggttgaaac
    49921 tagtgactac atttctaaga aattggttgc agctggtgtt cctcacgaag tcttgaatgc
    49981 caaaaaccac tatagagaag cccaaatcat catgaatgct ggtcaacgtg gtgccgttac
    50041 catcgcaacc aacatggcgg gtcgtggtac cgacatcaag cttggtgaag gtgttcgtga
    50101 acttggagga ctttgtgtta ttggtacaga acgtcatgaa agtcgtcgta tcgataacca
    50161 gcttcgtgga cgttcaggtc gtcaaggaga tccaggtgag tcacaattct acctatctct
    50221 tgaagatgat ttgatgaaac gttttggttc tgaacgcttg aagggaatct ttgaacgttt
    50281 gaacatgtct gaagaggcga ttgagtctcg catgttgacg cgtcaggttg aagcagctca
    50341 gaaacgtgtc gaaggaaata actacgatac ccgtaaacaa gtccttcaat acgatgatgt
    50401 catgcgtgaa caacgtgaga ttatctatgc tcaacgttac gatgtcatca ctgcagatcg
    50461 tgacttggca cctgaaattc aggctatgat caaacgcacg attgaacgtg tcgttgatgg
    50521 tcatgcgcgt gccaaacaag atgaaaaact agaggcaatt ttgaactttg ctaagtacaa
    50581 cttgcttcct gaagattcta ttacgatgga agacttgtca ggcttgtctg ataaggccat
    50641 caaggaagag cttttccaac gtgccttgaa ggtttacgat agtcaggttt caaaactacg
    50701 cgatgaagaa gcagttaaag aattccaaaa agttttgatt ctacgagtgg tggataacaa
    50761 gtggacagat catatcgatg cccttgatca attgcgtaac gcggttggac ttcgtggcta
    50821 tgctcagaac aaccctgttg ttgagtatca ggcagaaggt ttccgtatgt ttaatgatat
    50881 gattggttcg attgagtttg atgtgacacg cttgatgatg aaagcacaaa ttcatgaaca
    50941 agaaagacca caggcagaac gtcatatcag tacaacagcg actcgcaata tcgctgctca
    51001 ccaagcaagt atgccagaag atttggattt gagccagatt ggacgcaatg aactttgccc
    51061 atgtggttct ggtaagaaat ttaaaaactg tcacggtaaa agacaataaa atgagatagt
    51121 ttagaggcgg atatcttgtg aaaagtaaat ttttactggg tatccgtttg ctttataagg
    51181 agatgagtta tggtatttac agcaaaaagc tctaaaataa atatagaaga agttcgtgcc
    51241 ttgtcaaaat tagaaggtca ggctttggag aggaaatcac agcgcgatca agagctagaa
    51301 gccattatac gtggagaaga tcagcgaatt ctcttggtaa tcgggccatg ctcatctgac
    51361 aacgaagaag ctgtccttga atacgctaag cgtttggcag tcctacaaga agaagtggca
    51421 gatcgtatct ttatggttat gcgtgtttat actgccaaac cccgtaccaa cggagatggc
    51481 tataagggct tgattcacca gcctaacgcg acagaagcgc ctagtcttat caatggaatc
    51541 aaagccgttc gccatcttca ctatcgtgtc atcacagaaa cagggatgac aactgctgat
    51601 gaaatgcttt atcctgaaaa ccttccgctt gtagatgatt tgatttctta catggcagtt
    51661 ggtgcccgtt cagttgaaga ccagcaacac cgctttgtgg caagtggggc aggattttct
    51721 actggtttta aaaatccaac ctctggaaat ctcaatgtca tgtttaatgg gatttatgct
    51781 gctcaaaaca aacaaagttt ccttttctta ggaaaagaag tagaaacaac tgggaacccg
    51841 ctttcacacg ctattcttcg tggtgctctt aatgagtatg gaaaaaatat tcccaactac
    51901 tattatgaca atttaattga taccattgcc caatatgaga aaatgggctt ggaaaatcct
    51961 tttatcatca ttgataccaa tcatgacaat tctggtaagc agtatattga acagatccga
    52021 attgtccgcc agaccttgat taaccgtgct tggaatgaaa aaattaagca gttcgttcgt
    52081 ggttttatga ttgagtctta tctggaagat ggtcgacaaa atgagccaga agtatttggc
    52141 aagtctatca cagacccttg cctgggttgg gataacacag aagctcttgt cagagaaatt
    52201 tacaaaacgt taggagaata agatggcatt tattgaaaaa ggtcaagaaa tcgatatgga
    52261 agtcatcaag gctgaaaccc aattgtctgc ggaagccttg agactcaagg aaagccgtga
    52321 cagggaattg gcagatatta tttcagggga agatgaccgt attctcttgg tgattggtcc
    52381 ttgctcttct gataatgaag aggcggtctt ggaatatgct cgccgtttat ccgccttgca
    52441 aaagaaggta gcggacaaga ttttcatggt catgcgcgtg tatactgcta agcctcgtac
    52501 caatggagac ggctataaag gattggttca ccagccagat acttctaagg ctccaagcct
    52561 gattaacggt ttgcaggctg tgcgccagtt gcattaccgc gtgattacag agactggttt
    52621 gacaacggca gatgagatgc tttatccgtc aaatctgatc ttggtggatg acttggtcag
    52681 ctaccatgcc gttggagctc gttctgtgga agaccaagag caccgctttg tggcttctgg
    52741 gattgatgca ccagtaggga tgaaaaatcc aacctcagga aatttgggtg ttatgtttaa
    52801 cgccatctat gctgctcaaa acaagcaaac cttcctttat catgggcagg aagttgagac
    52861 atcaggtaat cctttggccc atgttatcct ccgtggagca gtcaacgagt atggcaatta
    52921 tatgccgaat tactactatg aaaacctact ccaagccatt gaacgctatg aaaccatggg
    52981 acttgaaaat ccttttatcc tcattgacac caaccatgat aactcaggca agcaatatat
    53041 ggagcagatt cgaattgttc gccagacctt gcagaatcgt gattggaatg agaaaattaa
    53101 aaagacggtt cgaggattta tgattgaatc ttacctagca gatggtcgtc aaaaccaacc
    53161 agagatcttt ggttgctcta ttactgaccc ttgcctaggt tgggaaaata cagaggcctt
    53221 ggtagaagag atttatgtta ccttgacaaa ataagtgaaa aggatggagt tggggaatct
    53281 caactccttt tgatgagaat gatagttgga cacggaattg acatcgaaga attggcttcg
    53341 atagaaagcg cagttacacg acatgaagga tttgctaagc gtgtactgac cgctcaggaa
    53401 atggagcgct tcaccagtct caaaggacgc aggcaaatag aatatttagc tggtcgctgg
    53461 tcggctaagg aggccttttc caaggctatg ggaacgggca ttagcaagct cggttttcag
    53521 gatttggaag tattgaacaa tgaacgtggg gcgccttatt ttagtcaggc accattttca
    53581 ggaaagattt ggctgtctat cagccacacc gatcagtttg tgacagccag tgtcattttg
    53641 gaggaaaatc atgaaagcta gtccacatag accaaccaag gctctgattc atctgggagc
    53701 tattcgacaa aatattcagc aaatgggggc tcatatccct caaggaacgc tcaagttggc
    53761 tgtggttaag gccaatgctt atggtcatgg agctgttgcc gttgccaagg caattcaaga
    53821 tgatgttgat ggcttttgcg tttccaatat cgatgaagcc attgaactca gacaagctgg
    53881 actcagcaag ccaatcctca ttttaggagt ttctgaaatc gaagctgttg ctctagctaa
    53941 agaatatgac ttcaccttga cagtggctgg actggagtgg attcaagcac tcttagataa
    54001 ggaagtggac ctaactggat tgacagtcca cctcaagatt gattcaggga tgggacggat
    54061 tggttttaga gaggcaagtg aggttgagca ggctcaagat ttgctccaac aacacggtgt
    54121 tcgtgttgaa ggaatcttta cccactttgc tactgctgat gaggaatcag atgactattt
    54181 taatgcccag ttagaacggt ttaaaactat tttagctagt atgaaggaag ttccagagct
    54241 ggttcatgct agcaattctg caacgactct ttggcatgta gagactattt tcaatgcggt
    54301 tcgtatggga gatgccatgt atggtctcaa tccaagtgga gcggtcttgg ctttgcctta
    54361 tgatttgata ccggccttga ccttggagtc tgctctggtt catatcaaga cagttccagc
    54421 tggtgcttgc atgggctatg gagcaactta tcaaacggat agcgagcaag tcatcgcgac
    54481 cgtgccaatc gggtatgcag atggatggac aagagacatg caaaatttct ctgtcttggt
    54541 agatggccaa gcttgcccaa ttgtcggcag ggtttcgatg gaccaaatca ctattcgatt
    54601 gcctaagcct tacccactcg gaaccaaggt aaccttgatt ggctccaatg gggataagga
    54661 aatcactgca actcaggtag cgacctaccg cgtaaccatt aactatgagg tggtttgcct
    54721 cctcagcgac cgtattccga gagaatatta ttagaaaaga aaggagtgga gcatgaatct
    54781 acatcaaccc ttgcatgtct tgcctggtgt gggaccaaag tcagcagaaa aatacgccaa
    54841 actaggaatt gaaaacttgc aagatctctt gctctacttt cctttccgtt atgaagactt
    54901 caaaaccaag caggtgctgg aactagaaga cggtgagaag gcagttcttt ctggtcaggt
    54961 agtgactcct gctagtgtcc agtattatgg tttcaagcgc aatcgcctgc gttttagtct
    55021 caagcaggga gaggtcgttt ttgcggtgaa tttctttaac cagccctatc tggctgataa
    55081 aatagagttg ggagcaaccc ttgctgtctt tggaaaatgg gaccgcgcta aggctagtct
    55141 gactgggatg aaggttctgg ctcaggtaga agatgacctc cagcctgtct atcgtctggc
    55201 tcagggaatc agtcaggcca gtctggtcaa ggtcatcaag acggcttttg atcagggact
    55261 ggacctcttg atagaagaaa atctgcccca gtctttacta gacaaataca aactcatgtc
    55321 ccgttgtcag gcagtccgag ctatgcattt tccaaaggat ttggcagaat acaagcaggc
    55381 tcttcgccgt atcaagtttg aggaactctt ttatttccaa atgcagttgc agatgctcaa
    55441 gtctgaaaat agagttcagg gaagtggtct ggttctgaat tggtctcagg aaaaagtgac
    55501 agcagttaaa gcaagtcttc cttttgccct gacccaagct caggaaaaga gtttgcagga
    55561 aattttaact gatatgaagt ccgaccacta catgaatcgt ctcctacaag gagatgtggg
    55621 gagcggaaaa acggtagtcg ctggcttggc catgtttgcg gcagtgacag caggttatca
    55681 ggctgcccta atggtaccaa cagaaatcct cgcagagcaa cactttgaga gtttacagaa
    55741 cctttttccc aatttgaaac tggctctctt gacaggttcc ttgaaagctg cagaaaagag
    55801 agaggtcttg gagaccattg ccaagggtga ggctgatttg attataggaa ctcacgctct
    55861 gatacaagat ggggtggagt atgctcgtct tggtttgatt attatcgatg agcagcaccg
    55921 ttttggtgtg gggcaaaggc gtattttacg ggaaaaaggc gacaatccag atgtcctcat
    55981 gatgacggcg actcccattc cacggacgct tgccatcaca gcctttggag atatggatgt
    56041 ttccattatc gaccagatgc cagcaggtcg gaagcctatt gtgacgcgct ggatcaaaca
    56101 tgagcaacta cctcaggtct tgacttggtt agagggggaa attcaaaaag gttcccaagc
    56161 ctatgtcatc tctcctttga ttgaagaatc agaagctcta gatttgaaaa atgccattgc
    56221 cttatcagag gagttgacga ctcattttgc aggcaaggca gaggtggctc ttctacatgg
    56281 taggatgaag agtgacgaaa aagaccagat catgcaggat ttcaaggaga gaaagacgga
    56341 tattctggtt tcgacgacgg ttattgaggt tggggtcaat gttcccaatg cgactgtcat
    56401 gattatcatg gatgccgatc gcttcggtct cagtcaactt caccagctta gaggtcgtgt
    56461 cggtcggggg gacaagcagt cctacgctgt tctcgttgcc aatcccaaga cggattctgg
    56521 gaaagaccgc atgcgcatca tgacagaaac gaccaatgga tttgtccttg cggaggaaga
    56581 tttgaaaatg cgtggttctg gtgagatttt tggaaccaga cagtcaggac ttccagagtt
    56641 ccaagtggct gatattatcg aagattttcc gattttagaa gaagcaagaa aggttgctag
    56701 ctacattagt tctatagaaa cttggcaaga agatccagag tggcgcatga ttgcccttca
    56761 tctggaaaag aaagaacatc tggattaagc tttctctaag gaaaacttaa gctagttttt
    56821 agatttggct cttatactaa agtcatcaaa aagaaacgag gactctcata tgacagtaac
    56881 gattaaagta aattaccaaa ccactttcca aaagaaggaa gca
//
LOCUS       DAVKQB010000006        49435 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKQB010000006 DAVKQB010000000
VERSION     DAVKQB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.006"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..749
                     /locus_tag="VW528_000353"
                     /pseudo
     CDS             1..749
                     /locus_tag="VW528_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(865..1668)
                     /locus_tag="VW528_000354"
     CDS             complement(865..1668)
                     /locus_tag="VW528_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9911236.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(2048..3652)
                     /locus_tag="VW528_000355"
     CDS             complement(2048..3652)
                     /locus_tag="VW528_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9911237.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNVKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEII
                     SEIMDESYTDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVND
                     IVNAQSNSLILIDEPEISLHPSAIYKFKEFLLQECLNKKHQIIITTHSTQLIKDFLRE
                     AVKLLVKNGEKVDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSE
                     NLKQNLVVRYIPGGANQIICNNILNSSYLDSDNHYFWFDGDQNTNVSESNNLMNYLEN
                     GVVISDKIPESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKY
                     VSYLPFPTPEFFLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQE
                     QRRAVSKIQPESSMFQCIKEKLEALF"
     gene            complement(3703..3867)
                     /locus_tag="VW528_000356"
     CDS             complement(3703..3867)
                     /locus_tag="VW528_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9911238.1"
                     /translation="MGKIVAIDLFNGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            complement(3948..6599)
                     /locus_tag="VW528_000357"
     CDS             complement(3948..6599)
                     /locus_tag="VW528_000357"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9911239.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVIAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(6592..7170)
                     /locus_tag="VW528_000358"
     CDS             complement(6592..7170)
                     /locus_tag="VW528_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9911240.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAEILQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(7170..7730)
                     /locus_tag="VW528_000359"
     CDS             complement(7170..7730)
                     /locus_tag="VW528_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9911241.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHIFPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLDFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(7717..8165)
                     /locus_tag="VW528_000360"
                     /pseudo
     CDS             complement(7717..8165)
                     /locus_tag="VW528_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            complement(8577..8972)
                     /locus_tag="VW528_000361"
     CDS             complement(8577..8972)
                     /locus_tag="VW528_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9911242.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQPAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(9068..9322)
                     /locus_tag="VW528_000362"
     CDS             complement(9068..9322)
                     /locus_tag="VW528_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9911243.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(9332..10666)
                     /locus_tag="VW528_000363"
     CDS             complement(9332..10666)
                     /locus_tag="VW528_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9911244.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYEYMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(10666..10899)
                     /locus_tag="VW528_000364"
     CDS             complement(10666..10899)
                     /locus_tag="VW528_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9911245.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            10930..11007
                     /locus_tag="VW528_000365"
                     /pseudo
     CDS             10930..11007
                     /locus_tag="VW528_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(11348..11530)
                     /locus_tag="VW528_000366"
     CDS             complement(11348..11530)
                     /locus_tag="VW528_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911246.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(11532..12311)
                     /locus_tag="VW528_000367"
     CDS             complement(11532..12311)
                     /locus_tag="VW528_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911247.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
     gene            complement(12591..12941)
                     /gene="rbfA"
                     /locus_tag="VW528_000368"
     CDS             complement(12591..12941)
                     /gene="rbfA"
                     /locus_tag="VW528_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9911248.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(13192..15984)
                     /gene="infB"
                     /locus_tag="VW528_000369"
     CDS             complement(13192..15984)
                     /gene="infB"
                     /locus_tag="VW528_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9911249.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKTEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(16001..16300)
                     /locus_tag="VW528_000370"
     CDS             complement(16001..16300)
                     /locus_tag="VW528_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9911250.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(16293..16586)
                     /locus_tag="VW528_000371"
     CDS             complement(16293..16586)
                     /locus_tag="VW528_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9911251.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(16608..17744)
                     /gene="nusA"
                     /locus_tag="VW528_000372"
     CDS             complement(16608..17744)
                     /gene="nusA"
                     /locus_tag="VW528_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9911252.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(17788..18267)
                     /gene="rimP"
                     /locus_tag="VW528_000373"
     CDS             complement(17788..18267)
                     /gene="rimP"
                     /locus_tag="VW528_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9911253.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMGKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(18393..19028)
                     /gene="trmB"
                     /locus_tag="VW528_000374"
     CDS             complement(18393..19028)
                     /gene="trmB"
                     /locus_tag="VW528_000374"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9911254.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQDPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(19025..19819)
                     /locus_tag="VW528_000375"
     CDS             complement(19025..19819)
                     /locus_tag="VW528_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9911255.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(19980..20591)
                     /locus_tag="VW528_000376"
     CDS             complement(19980..20591)
                     /locus_tag="VW528_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9911256.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            complement(20742..20975)
                     /locus_tag="VW528_000377"
     CDS             complement(20742..20975)
                     /locus_tag="VW528_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911257.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            complement(21017..21706)
                     /locus_tag="VW528_000378"
     CDS             complement(21017..21706)
                     /locus_tag="VW528_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9911258.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            complement(21758..22141)
                     /locus_tag="VW528_000379"
     CDS             complement(21758..22141)
                     /locus_tag="VW528_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9911259.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            complement(22770..23141)
                     /locus_tag="VW528_000380"
     CDS             complement(22770..23141)
                     /locus_tag="VW528_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911260.1"
                     /translation="MIKKNHILCEIVPGAVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            complement(23610..23729)
                     /locus_tag="VW528_000381"
     CDS             complement(23610..23729)
                     /locus_tag="VW528_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9911261.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(23833..23982)
                     /gene="blpO"
                     /locus_tag="VW528_000382"
     CDS             complement(23833..23982)
                     /gene="blpO"
                     /locus_tag="VW528_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9911262.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(24226..24429)
                     /gene="blpN"
                     /locus_tag="VW528_000383"
     CDS             complement(24226..24429)
                     /gene="blpN"
                     /locus_tag="VW528_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU9911263.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(24445..24699)
                     /gene="blpM"
                     /locus_tag="VW528_000384"
     CDS             complement(24445..24699)
                     /gene="blpM"
                     /locus_tag="VW528_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU9911264.1"
                     /translation="MNTKMMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            24979..27104
                     /gene="blpA"
                     /locus_tag="VW528_000385"
                     /pseudo
     CDS             24979..27104
                     /gene="blpA"
                     /locus_tag="VW528_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
     gene            27115..28476
                     /locus_tag="VW528_000386"
     CDS             27115..28476
                     /locus_tag="VW528_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9911265.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVISSIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGIFKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDAIVNKRARLPTGNPHQSILNRY
                     LIASQEQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            28533..28688
                     /gene="blpC"
                     /locus_tag="VW528_000387"
     CDS             28533..28688
                     /gene="blpC"
                     /locus_tag="VW528_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9911266.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(28732..30072)
                     /locus_tag="VW528_000388"
     CDS             complement(28732..30072)
                     /locus_tag="VW528_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9911267.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLLKFLL
                     GIIFTTFQFLAVSKYLSYFIEPLFGIGLSFLLLRGLPKKILIFYGLFPMILVELFYRG
                     VSYFVLPFLGQGIVDGDGNPIFLLIMIFVCFIVLVFLKWLDYDFTRLRREFLDTGFQK
                     SLTKINWAMGAYYLVMQSLSYLEYEQGIQSTTVRHLILVFYLLFFMGGIKKLDTYLKE
                     KLQEELNQEQTLRYRDMERYSRHIEELYKEIRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLRDSSQKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTVVSILCDNAIEASVEACQPHVSIAFFKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(30086..30823)
                     /locus_tag="VW528_000389"
     CDS             complement(30086..30823)
                     /locus_tag="VW528_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9911268.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(30828..31166)
                     /locus_tag="VW528_000390"
     CDS             complement(30828..31166)
                     /locus_tag="VW528_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9911269.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            31442..31771
                     /locus_tag="VW528_000391"
     CDS             31442..31771
                     /locus_tag="VW528_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9911270.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(31939..32988)
                     /locus_tag="VW528_000392"
     CDS             complement(31939..32988)
                     /locus_tag="VW528_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9911271.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(32985..33716)
                     /locus_tag="VW528_000393"
     CDS             complement(32985..33716)
                     /locus_tag="VW528_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911272.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            33784..34194
                     /locus_tag="VW528_000394"
     CDS             33784..34194
                     /locus_tag="VW528_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9911273.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            34204..34491
                     /locus_tag="VW528_000395"
     CDS             34204..34491
                     /locus_tag="VW528_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911274.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            34653..34892
                     /locus_tag="VW528_000396"
     CDS             34653..34892
                     /locus_tag="VW528_000396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911275.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(35214..36350)
                     /gene="dnaJ"
                     /locus_tag="VW528_000397"
     CDS             complement(35214..36350)
                     /gene="dnaJ"
                     /locus_tag="VW528_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9911276.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFATAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(36555..36809)
                     /locus_tag="VW528_000398"
     CDS             complement(36555..36809)
                     /locus_tag="VW528_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911277.1"
                     /translation="MLVTVERNGNVIYKEFTEVAVGKSKYDHTSVFKDVMIYFSDHIC
                     QELHLYKTQDPHYNKFEFDLAKRSLKITTRDNTTIFISNE"
     gene            complement(36922..38745)
                     /gene="dnaK"
                     /locus_tag="VW528_000399"
     CDS             complement(36922..38745)
                     /gene="dnaK"
                     /locus_tag="VW528_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9911278.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(39225..39749)
                     /gene="grpE"
                     /locus_tag="VW528_000400"
     CDS             complement(39225..39749)
                     /gene="grpE"
                     /locus_tag="VW528_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9911279.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(39776..40810)
                     /gene="hrcA"
                     /locus_tag="VW528_000401"
     CDS             complement(39776..40810)
                     /gene="hrcA"
                     /locus_tag="VW528_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9911280.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(40974..41411)
                     /locus_tag="VW528_000402"
     CDS             complement(40974..41411)
                     /locus_tag="VW528_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9911281.1"
                     /translation="MHLKGWRYRTSRFGLFYFDQCQPDDVIYRIYDSRFLKKYKHELQ
                     DFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMSKGLKWEVMRSRLRSCTTTFLGGLV
                     VCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFRLKRKYRVDE"
     gene            42091..44424
                     /locus_tag="VW528_000403"
     CDS             42091..44424
                     /locus_tag="VW528_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9911282.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVSEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            44437..45900
                     /locus_tag="VW528_000404"
     CDS             44437..45900
                     /locus_tag="VW528_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9911283.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFKDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            45900..47468
                     /locus_tag="VW528_000405"
     CDS             45900..47468
                     /locus_tag="VW528_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9911284.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLAR
                     FIDPKTVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRV
                     LSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIV
                     DKIEQFFAHIDALI"
     gene            47525..48322
                     /gene="psrA"
                     /locus_tag="VW528_000406"
     CDS             47525..48322
                     /gene="psrA"
                     /locus_tag="VW528_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9911285.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            48333..48941
                     /locus_tag="VW528_000407"
     CDS             48333..48941
                     /locus_tag="VW528_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9911286.1"
                     /translation="MVKLTTCYFCDYGSYYGNKDETTSYPIYEIPEAWRYIKFASLVN
                     FRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTL
                     LMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAI
                     KGKTLNSTSISELLIPISNHEEMKRIIFKVDLLFQKVSQLFE"
     gene            complement(48895..49434)
                     /locus_tag="VW528_000408"
     CDS             complement(48895..49434)
                     /locus_tag="VW528_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9911287.1"
                     /translation="MNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKALTKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQL"
BASE COUNT        15348 a        10257 c         8898 g        14932 t
ORIGIN      
        1 aaaacagata aggaccaaga gcttaaagct gaaattcaat ccatctttat cgaacacaag
       61 ggaaattatg gttatcgtcg gatttattta gaactaagaa atcgtggtta tctggtaaat
      121 cataaaagag ttcaacactt gatgaagtac tcaatttaca agctaaaatg cgacagaaac
      181 gaaaatattc ttctcataaa ggagacgttg gcaagaaggc agagaatctc attcaaggcc
      241 aatttgaagg ctctaaaaca atggaacagt gctacacaga tgtgacagaa tttgccattc
      301 cagcaagtac tcaaaagctt tacttatcac cagttttaga tggctttaat agcgaaatta
      361 tcgcctataa tctttcaact tcacccaact tagaacaagt acaaacaatg ttggaacagg
      421 cattcacaga gaagcactat gagaatacga ttctccatag tgaccaaggc tggcaatacc
      481 gacacgattc ttatcatcgg ttcctagaga gtaagggagt tcaagcatcc atgtcacgca
      541 agggaaacag ccaagacaac ggtatgatgg aatctttctt tgggattctg aaatcggaaa
      601 tgttttacgg ttatgagaag tcgtttcagt cgcttaagca attggaacaa gctattgtag
      661 actatattga ttactacaac aataaacgaa ttaaggtaaa actaaaagga cttagtcctg
      721 tgcaatacag aactaaatcc ttcggataaa ttaattgtct aactgtttgg ggtcagtaca
      781 cctaaaactt tgatgatata cgttttcttg tgagaatatt tacttcattt ttgcctaaaa
      841 ttcaatgttt actcagtatt tggattatga aaaatcaagg tctaaatcta gatacatttt
      901 ttctgaagac aaatcatttt gaccaccgag caagagattt tcaaaaaaag ctgttaaaaa
      961 ctcaggacgt cgctgtaaaa tctttgcatt atctaatacc aaggcatcac gaaaatattt
     1021 ggaatgttgc tgaaatggtg tattatcaat atcaaaacca aactcacgaa gatactgaat
     1081 caaaaagacc gttactgtcc gagtgtttcc ttcgcgaaat ggatgaatct gccagattcc
     1141 tgaaataaaa tgctggattt gtttaaccac atccgcctga gttagtgtcg catatgcaac
     1201 ttgtttttcc tgattaaaat cataatctaa ggtcatttga atcatggagt aatcagagta
     1261 cacaacactt tcaccattca aaacaggttc attctttgtg atattggtct gacgaaattg
     1321 ccccaccgga atagagggtt caaatatatc ttgaaacaac tccttatgaa tagcaagtaa
     1381 ggtcgcagga ctaaagctaa agcctcttcg agacaatagt tctacaatac gtagagaaac
     1441 caagtctgcc tcttccgtac ttgcatcaat agtatggtga taagccgttg catcctcata
     1501 aacctgctca taagtcagtt ctccccggga ctgtttctca gccaaagatt ccatatacgc
     1561 tgatggcact agattgtcaa ctttctgcag accaaaacct atccgccata aatcacgctt
     1621 cgcttcataa gacaagtttg gattgtcaat gttgtaagtt ggttgcatag aaatatcctt
     1681 tcaattgttt ttctgtgtca tcggtaaatg gtgaaatggg tatggaattg gcgaaaatta
     1741 taaaaatcct tcacaataag ttctatgaaa tatatatact tcatcttaat agtgagcaag
     1801 ctaaacttag catttcatgc cctcatatgg gatgttcttt gactaaataa tatgattatc
     1861 gagatatatc tggataaatg aactaataag tctgacgcgc atacttatca aactcattgg
     1921 catacacctc tatgaactcg ttggtctgtt caaatcccaa cacattacct gagaagaaag
     1981 ttgcaatgtt gtttttggtg cgggtttgaa tttaaaaaat ttgttatgta gtacctaatc
     2041 taaggaatta gaacaatgcc tctaattttt ctttaataca ctgaaacatt gatgattctg
     2101 gctgtatttt tgaaacagct cttctttgct cctggaaaat atcttcagaa gttatattct
     2161 ctattcctaa cgctacttga gttttttttc taaaatattc ttttccgttg ccatctttag
     2221 aaaaatcata accttcccta tctacgctgt tacacaaatt agctaaaaaa aactctgggg
     2281 ttgggaaagg aagataagaa acgtatttag cccaataatc tataaagctt ctttgtttcg
     2341 caattaattc aatattattt ttttgccctt tattacctga aacattaaat ttaattggac
     2401 atcccgttat caattttata atatcatcaa gatttttatt atctgattca ggaattttat
     2461 ctgatataac aacaccattt tcaagatagt tcattaaatt atttgattca ctaacattag
     2521 tgttttgatc tccatcaaac caaaaataat ggttatcgga atctaaatac gatgagttta
     2581 aaatattatt acaaattatt tgatttgctc caccaggaat atatctcact actaaattct
     2641 gtttaagatt ctcactacct gaatgagtga taacaaactc tagaatatat ttagctagtc
     2701 tatcttcaac ataaatcatc ttcctagaat gatacacatc acctaattca aaaaatgcat
     2761 cctgataatc aatattttca ataacatcta ccttttctcc gtttttcact aaaagtttca
     2821 cggcttctct aagaaaatct tttataagtt gtgtagaatg tgtagtgata ataatttgat
     2881 gttttttatt taaacactct tgaagtaaaa actctttaaa tttatagatt gcactcggat
     2941 gaagtgagat ttcaggttca tctattaata ttaatgaatt tgattgcgca tttactatat
     3001 catttactaa caaaataatt ctagcctcac ctgttcctgc aaaagcctcg gaatattctt
     3061 ttccagattt tttcatccaa atagttttgg aagcttttat atcatcacct tttgaataca
     3121 acttatgtgt taaaatttga atgtctgtat aagattcatc cattatttca ctaataattt
     3181 cacaaacttt atcatcaact ttaacattat ctataaccat ttccttttta taacgcgtat
     3241 aactacttgt attattcttt aaaatatcag caactggttt agatcgtaat cttataaaat
     3301 cttgtttact acgttgagta gaaatttttt taaaattata gtgatagaaa aataaatcaa
     3361 aagcagaaac atattcttta caatcacaaa agacaacatt tttttcaatg ccatcccatc
     3421 tgtctgtcga agaacttcca atatatttat ttttgggtaa tctttccatc tcatattgtt
     3481 tttgaggagc atatggttcc caataatcta atcctttttt tgttccagaa cggcctttaa
     3541 gaacttctac atttctagaa gctttaacgt tataatatga atagattaaa cattgtttcc
     3601 catccacttc atctatttga tcaacatttg tactaaacca atattcagac acacttttat
     3661 tggctggaga accatataaa gcttgtaaaa ttgaagtttt atttactcca tatctattac
     3721 agacacctca ggattattta acttataagt tttaacagct acggaatcaa tttcaacagc
     3781 aacttgaaca tctatgcctg attttttaag gccacttgta gtgccacctg caccgttaaa
     3841 taaatcaata gcaacaattt tccccatagt attctcctaa agtttctcct ttttattata
     3901 acattatcaa atgtaaaacc caacccgata gggttaggtt tttaacatca tttcaccaac
     3961 ttcttcatct catcaatacg tgctacggtc gcatcatact tggcttggta gtcggcttgt
     4021 ttgtcttttt ctttttggac aacttctggt ttagcgttgg cgacgaagcg ttcgttgctg
     4081 agttttttgc caaccatgtc gagttctttc tgccatttgg caagttcttt ttccagacgt
     4141 gccaattctt cttcgacatt caagaggtct accagtggca agaagatttc ggctcctgta
     4201 ataacgcttg acatagccag ttcaggtgca gggatggttg atgcgatttc caagtgttct
     4261 ggatttgtaa agcgtttgat atagttgaca ttgctgttaa agaaggcttc caagtcgcta
     4321 tcgcttgtct taacaaggat ggtgataggt ttgcttggtg ctacatttac ttccgcacgc
     4381 gcattacgaa cggcacggat caagtctttg agactttcca caccagtgtg ggcagcaagg
     4441 tcctcaaagg ctgggttgac agttgggtat gctgctgtca cgatagagcc ttcagcgtac
     4501 tgaccaaaga tttcttctgt cacgaacggc attattgagt gaaggagacg aaggatttta
     4561 tccagcgtat agaggagaac agatcgagta attaccttat cgtcttcatt gtcgctgtaa
     4621 agaacttcct tggtcaactc aacataccag ttggcaaatt cttcccaaat gaagttgtaa
     4681 aggatatgac cagccacacc aaactcgaac ttatcaaagt tttcagtaac ttttgcaatg
     4741 gtttcgttga gattgtggag aatccagcgg tccgtcacat taccagcctc acctgttgca
     4801 acttttgtga cattgtcatg cgccacatcc agcgtcaaac ctccattgtt catgaggata
     4861 tagcgagaaa tgttccaaat cttgttaata aagttccatg aagcatccat tttctcataa
     4921 gagaaacgca cgtcttgtcc tggcgcagaa ccgtttgaaa ggaaccatcg aagggcatcg
     4981 gcaccgtatt tctcgataac atccattggg tcaatcccgt taccgagaga cttagacatc
     5041 ttgcgtcctt gctcgtcacg aatgagaccg tggataagga cgttttggaa tggttgacgg
     5101 cctgtgaatt ccaatgactg gaagatcata cgagacaccc agaagaagat gatgtcgtaa
     5161 cctgttacca aggttgaagt tgggaaataa cgtttaaagt cttctgagtc gacttcaggc
     5221 cagcccatgg ttgaaaatgg ccagagggca gaactgaacc aagtatccaa gacgtcttcg
     5281 tcctgagtcc atccgtcacc ttctggagct tcttcgccga catacatttc accatcagca
     5341 ttgtaccagg cagggatttg gtgaccccac caaagctgac gagagataac ccagtcgtgg
     5401 acattttcca tccattgaag gaaggtatcg ttgaaacgag gtgggtagaa ttcgaccttg
     5461 tcctctgtgt cttggttggc aatggcgttc ttagccaatt ggtccatctt gacgaaccat
     5521 tgagtagaca agcgtggctc aactacgaca cctgtacgtt ctgagtgacc aacactgtgg
     5581 acacgttttt cgattttgac aagggcaccg atttcttcca acttagcaat gactgcctta
     5641 cgagcttcaa aacgatccat gcctgaaaat tcaaaggcaa gctcattcat agttccgtcg
     5701 tcgttcatga cgttgacttg tggcaagtta tgacgttggc caaccaagaa gtcatttgga
     5761 tcgtgggcag gtgtgatttt cacgacacca gtaccaaact caggatctgc gtgctcatct
     5821 ccaacgattg ggatgagttt attagcgatt ggaaggatga cgtttttacc aatcaagtcc
     5881 ttgtagcgcg ggtcttctgg attaaccgca accgcaacgt ccccaaacat agtctcagga
     5941 cgagttgtag caacttcaag ggcgcgtgaa ccatcttcca gcatgtaatt catgtggtag
     6001 aaggcacctt ccacatcctt gtgaatcacc tcaatatcag aaagggctgt gcgagctgct
     6061 gggtcccagt tgatgataaa ctcaccacgg tagatccagc ctttcttgta aaggtccaca
     6121 aagaccttac gaacagcttt tgacaaacct tcgtcaagag tgaaacgctc acgagaatag
     6181 tctacagaga gccccatctt gccccattgt tccttgatgg tagtggcata ttcgtctttc
     6241 cattcccaga ctttcgtcaa gaaagactca cgaccaaggt catagcggga aatgccctca
     6301 ccacgcaagc gctcctcaac cttagcctga gtcgcaatac cagcgtggtc cattcctgga
     6361 agccaaagcg tatcaaaacc ttgcatgcgt ttttgacgga tgataatatc ttgcaaagtt
     6421 gtatcccaag cgtgaccaag gtgaagttta cctgtaacgt ttggtggtgg aataacgatt
     6481 gaataaggct tagccttttg atcgcctgaa ggcttgaaaa catcagcatc aagccatttt
     6541 tggtaacgac cagcctcaac ctcggctgga ttgtatttag atgaaagttc tttagacatg
     6601 tgtgtgtcct ttctctattt tgtttatttt attttgaatt tgcttagcag cttcttctgc
     6661 agacaaattc gtattattta ttttaaagta gtggtgcaac tcattcggtt gatattggga
     6721 atttaattga agtatttcag cggtctctat aatttctctt tcagatacct caatatgtcg
     6781 ttttaagggt ttgtacttta atcgattctc cgttcgattt cgacgtatgc actcttcaag
     6841 acttgtttcc aactcaacaa aaagaatctc ttgatgataa tcatccaaca aactctgaat
     6901 ttgctttaaa tatatcagat gatactgatt tgaaaaatca attacatctg ttaaaattac
     6961 tgatcgctga tttcttgcac tcgctccaag gaatgaaaaa gtgattccac gaacaaattc
     7021 ccacatttcc tctgtatagt cctgatagat ttctagtgca aaatcgatgg cttgatggtt
     7081 ataaaatagg gtagcatccg tcagtcgaga taattcttga ccaatggtca tttttcctga
     7141 tgctggagca ccaatgatga aaagatgcat caaatcacct cccattcact cttcagcaag
     7201 gcatatctta aatcatcaca gcagttgcct tgagcatctt tgcggtctct tatgcgagct
     7261 tcgagggtaa agtcaagctt ttccgcgact cgtttacttt gaaggttata tccaaagcaa
     7321 gttagttcaa tcttgtgaag acccaaatct ttaaaggcta agtcaatcaa ggcacgcgca
     7381 gcttctggca cataacctcg cccccaatag tctgggtgta aggtatagcc gatttccagc
     7441 acatcatctt catggcgatg gttgaaatcg acagagccaa cgatcttatc cgttcctttg
     7501 acgacaatcc cgtagcctgc tgggagattt tccttttgat tacgctccgg aaaaatgtgc
     7561 tccagataat aaatctcatc ttccaaggtc ttgactggag gaaaacctgc tggataggcg
     7621 acctctggca aactagcata gtcaaagata tcctcagcat ccgccacagt acggactcgt
     7681 aaaacgagac gttctgtttc gattttatct ggcagctcag ttcttgccat ccttcttcct
     7741 cgcttttttg atgaaactgc ccttcatatc tacacgcttg tccagatagc aataaacgcg
     7801 ctgatatcca tctcccatga aataggttgg gcaaacagtt gatttttaaa atgtcccttt
     7861 tcatccagga gttctggggc aacaagtcgc tcaagaatct tggcaaagat gtggcaaata
     7921 ccgtattcct caacaatcct atctacccga taatctaaaa caagtggaca ggcgtctaaa
     7981 ataggagtct gagttcgttc agaaatttca taatgcactc ccaaacgttc caatttctcc
     8041 tgatgactga taaaaccagc ctgctccatc gcaagcatag aagtttcatc agaaatattc
     8101 acagtaaatt tttgatattg tttgatctgc tctgcggcat tctctctcgc aacgactcca
     8161 atcacaaccc aatctcctag actataagag gaactacagg tcatgatgtt atagctaaaa
     8221 ttctgatctt gatatcctaa aataaaaaca ggaaaaccat aatatagttt attagtttta
     8281 aatgattgtt ttataacaac ctcctttgac taagacacaa aagaaaagcc ctgccatcta
     8341 catgacaggg acgaatgtgt ttatccgcgg taccacccaa tttcgggcca agcccgcatc
     8401 tttagtcttt aaaataaaaa gacagaaata tttcattttt tcagtcatta gcaaccagtt
     8461 ttgacacatt tgtggacttc tcagcgccgc cactttctgt aaaatgtggg attcaaaaca
     8521 cctctaatgg ctctattgta gcaagttttt ctcggaaatg caaggctcaa gaaaaattac
     8581 ttgaacttga tgccattttc tttcaatttc ttgatggtag ctgggccgat tcctttcaag
     8641 gcaaggagtt ctttttcagt ccagtttttg aaatctgaag cagacttgat tccttcatca
     8701 tagaaagtct tggcacgatc aagtggaagt ccccccaagt ttgctgcaaa atcttctaca
     8761 gaattagcta ctttttgagc ttgctctttg gtagcttcta ctgcttgttc aactttttta
     8821 gaaacagcct taccagctgc gcttgctggc tgttctgcac gcttcacgac tttttttgtc
     8881 tcatctacaa ccttagtcac agtacttgaa aaggcacctg cgcgacggag gccatttcgt
     8941 aattgttttt tacgattgag tttctttgac atgataaaat cctttcaata aaaaatccct
     9001 gctctgacaa ggatttaaca taaacattct atcaagattt tagtgaaaaa tcaagaatct
     9061 ttctcgttta ataatttcgc tcaccaaaca agccttctgg caaatcatgg aatcccttgc
     9121 ctgctttgaa gttttcaaac ttaccaagca agaactgata agcatccaag cgactctcgt
     9181 agcgaatcat ggcatctttg gcacgctcgt cctggtcttc ttcgtaaact ttttgacttt
     9241 ccttgtgcgc catgtcgtta atcatgattt gggtattgac ccaagcttca aattccttca
     9301 taaattcttg ttcgtaactc attttttctc cttattctaa tccacgaaaa tagtccgtat
     9361 caatctcacg gtagggctgg atatcctgaa catactccaa cactccttgg aattctccgt
     9421 cttcatcgtg tactgcagca taggtgatgt ggacaaactt acctcgcgac tcagacttga
     9481 accacatttc atacttgtct ttgcttccct cacgaagccc cttcatgata gttttgacct
     9541 tgtccaagta cttaggcgga tggcagagtt cgacattgcg cccgacttga gacggcgtcc
     9601 gtttgaaaat catctcatca gctggcgtat tgtcattgta atactggaaa atatcatctt
     9661 tattgacaaa ggtaatctcc atagggaggt gattgaggat gagattggcc tgctcgactg
     9721 aaagatagcc attaccaaaa gcctgttgac tatggcggtc cagcacagct tccttttcct
     9781 taggggtaaa ggtaatggta aaatggcctt ctggcgtatc tatgacttgt tgaacttgac
     9841 cttctgccgt atctagctgt acaggctcct ctgcaatctt ttcctcaata aagctctgtc
     9901 gttctggcac ccatttctct gacggacgga tgatggcata gccataggca tcgctctcct
     9961 ccgcaatctg aagccagtca tcctgagtaa aagactcaag gagaatcatg aggaggatgg
    10021 actcttcctt gaaaatcata ctttcaaact ctgtcgcaaa agcttcaaaa gcttccttta
    10081 cactgctaat tgacacttct ggtagtgact tggctgtcgt tagagctgtt tgaaagagtt
    10141 ccctaatctg atcatccact ccccacataa ctttgggagg tgaatcgtgt ccatagcgct
    10201 ccatgatagg aaagaagagt tcttccttac gttggtaatg gatgtcaaat tgacccacaa
    10261 gtcccatctg acgcaccaaa cccttacgca tctccgccag catttcctcg tcttccatat
    10321 actcataggt atctaacaat ctacgaatgc gaatcaaggc cgcacggaga gccagatttt
    10381 cttctttgaa gacacgaact gggtgacctg gatgctcagt atctgaaact tcgacacctt
    10441 tgatagcatt tttaaaaaga ttggcatgga catcacagag ttccataaca tcttcaaagg
    10501 tgacacctga gtctgagttc atcagctcgt gctccataag ggaaatctcg atggctgaga
    10561 cacccgcaaa ggtcgcatca aagcgctctt gaaccgactc aggagaggcg ccattgtgca
    10621 attctaacaa aatatcccgt aggacatgaa tccgttcatc tgccattagt ctaatccaat
    10681 cacttcgtag ccattcgctt ccagtgtgcg tacaatcttg tccataggag ttcctgctag
    10741 cttagaaccc tgtttgagcg ataccttgcg accaactgta ttgcgcatta agggattggc
    10801 aaggggttta aaacccaact ccactagaat ttccaagact tctggatgct tgtccaccac
    10861 ttctgcaaca ggaattgaca catcgatgat attgtccatg acgacctcca ttgtatgttc
    10921 taatactcaa tgaaaatcaa agagcaaact aggaagctag ctgcagactg ctcaaaacag
    10981 tgctttgagg ttgtggatag aactgacgaa gtcagctcaa aacaccgttt tgaggttgta
    11041 gatagaactg acgaagtcag taaccatacc tacagcaagg tgaagctgac gtggtttgaa
    11101 gagattttcg aagagtataa aatgatttta ctgcttatat tataccatat ggagagagat
    11161 taaaaaacta gcagtggggt tcctgctagt tttctgcgac ttggattacc atctagataa
    11221 gtttaaagaa ccaatccaat atcttgtaaa acagcaattg aactgcaaaa gaaatgatca
    11281 tccaaaagaa tttcacaatc ccaataatcg gtttgataaa aagaatagca agaagaccaa
    11341 aaaaatttca ttctctctcc tctggcttaa tgagccaccg agccgtatcc attagcttat
    11401 ctgcaataaa aagtttgccc agaccaacaa cgacatggtc agctttcttt atcgttttca
    11461 tcacttttac accttgcacg gtcaaaaagt aattaccata aattttagct agagttttta
    11521 tgagtcccat attactctcc ttcgatgatt tcagcctctt ttcggattgt ttcaacatct
    11581 tcttgatatt gaacttcact atagttaact agcttggaca attctttctg caagctttca
    11641 cccatcggtt tcatagttgc aaaggttaaa ccacctgaaa tgataccacc caagatagga
    11701 ataaatttcc ccattccttt ggccagccct cccttggtaa gattcacacc aaatattctt
    11761 aagacttttt tcaaaatagg ataccaaagc gtcttagtta aagctttatt aggtactgtt
    11821 ttcattacct gtttggcaat tgttatacca ccagcacgta gcaaagcagc agttccattc
    11881 acccccaaca tgacgcctag ataaagcaag agggtatttt gagcatcttc actcaactca
    11941 tcgcgtgaag cccaaagatc ctcataacca taaatataac ctaattcttg ggccaatttc
    12001 agagagaaag cataaaattg agccacatca gctggaatgg taattgccat agcaagccct
    12061 ccaggtaatc ctgccaaaac agaagtccca ctcgctaata aaacattatc cctaatacag
    12121 gcattagcca ctcgatctaa gatttcttga gataagagag acgttggacc ttgttccaat
    12181 aaggtaggaa tgtcctgtgg acccaattct ttggaaaact tatccactaa aaatttcgaa
    12241 cgatcaacct taacaactgg tagttttacc acttgttgta atacttgcat agctaagtct
    12301 tgttcagcca tatacaaact ccttatttct tttttacacc ttatcatatt atatttccta
    12361 caaaaaaagc attttctaat tagataacat taaaaaatac agaacccctt tttttatagt
    12421 aaaatgaaac acgaacagga caaatcgatc aggacagtca aatcgatttc taacaatgtt
    12481 ttagaagtag aggcgtacta ttctagtttc aatatatttt aaaagaatat ttttccattt
    12541 caaattcaac ctattttcct ccaccaaaaa agaggggcaa cccctcttct ttagttctta
    12601 tccagattgc gtagcatctc gtcaatcttg tttccatact cgatggactc gtctttgacg
    12661 aaggtcaaat ctgggatttt gtacaatttc aaattgcgac caagttcacg tttgatggta
    12721 ccagttgctt tttcaagccc gatttgggct ttttggttat ccgaagcaag gttactcaaa
    12781 atggtgtaat aaaccttggc aacagacaag tcacccagca tctgaacatc tgtgatggtc
    12841 acaccttgga cacgtggatc acggactttc ttttgcaaaa tctcattgac ttcacgcttg
    12901 atttccatgc ccacacgatc tgtacggaaa tgatttgcca tgatattcct ttctctactt
    12961 tgaaccaaaa gctaggccag cagacctagc tattttaaac ttcttgattt tcagtttaaa
    13021 ttgaaacgaa gttcaatttg aagtcatctg cttctttcgc cgtggtgaaa gtgatggaac
    13081 tgatttatca gtcccatcac aggggatttt tgagacccta ggctcaaaaa taagcaatga
    13141 aaccatcggt ttgcttgcgt ccctcaccac ctaagaaagg agcaaaaaat cttatctctt
    13201 gatttcttcc atgacatacg cctcaatcac atcatccatc ttaatatcat tgtagccgtc
    13261 gatcatcaat ccaccttcac ggccgtttgt aacttcctta acgtcgtctt tatagtgttt
    13321 caagcttgcg agttcaccat catagataac gacaccatca cggataacac ggactttaga
    13381 gtcacgggca accttaccgt tgataaccat aaatccaccg atagttccca ctttagacac
    13441 cttgaaggtt tcacggataa ctgcttcacc aataactttt tcttcaaatt ctggatcaag
    13501 catccctttc atagcttctt ccatctcttc gataaccttg tagataatgc tgtgaagacg
    13561 gatttccaca tcgtcagctt ctgcttgttg acgagcttgt ggtgtagggc gtacgttgaa
    13621 accaacgata aaggcatttg aagcttcggc aagggtcacg tctgattcgt tgatagcacc
    13681 gaccgctgag tggacgatag tcactttgac accttccacg tcaatctttt gaagtgaggc
    13741 agaaagggct tcaacagaac cttgtacatc agccttgatg ataacattaa cagatttgag
    13801 ttccccagct ttaagggtat caaagaggtt ttcaaggcta acacgttggg tagcttgacg
    13861 ttgtttcatg agggcacgtt tggcacgctc ttcacctgct gcacgcgcag atttttcatc
    13921 ctcgtaaacg gcaaagtggt cacccgccat cggtgcttcg ttcaaacctg tgatagagac
    13981 tggtgttgat ggtccagcaa ctttaacacg acgaccaagg tcgttggtca tagcacggac
    14041 acgaccgaag gtatttccga caacgattgg gtcttgaaca ttcaaggtac cttgttgtac
    14101 aagaagggtt gcgaccgcac cttttccttt atccaagcgc gcttcgataa ccgtaccgat
    14161 tgcacgcact gttgggtctg ccttgagttc ttggatttca gctacaagaa ggactgtttc
    14221 caacaattct tcgatatttt ggttgaattt agctgagatt tcaacaaatt cagaatctcc
    14281 accccaagca gttgacataa ctccatgctc tgccaattca ccgataacgc gttctgggtt
    14341 agcacctggt ttatcaatct tgttaatggc tacgatgatt ggaacgttgg ccgcttttga
    14401 gtggttaatg gcttcgatag tctgaggcat aaccccgtca tctgccgcta cgaccaagat
    14461 cgtaatatcg gtaacagaag caccacgcgc acgcattgat gtaaaggccg cgtgtcctgg
    14521 tgtatcaagg aaggtaatct tcttaccatt ttccacgatt tggtaggcac cgatatgctg
    14581 agtaatacca cctgcttcac ctgtcgcaac acgtgagtta cgaagagtat ccaaaagggt
    14641 tgttttaccg tggtcaacgt gtcccatgat agtaacaact ggtggacgct caaccaattc
    14701 atcttcattg agataaccat cttcgacaaa gaaacgttcg atgtcagcat tatccacttc
    14761 aaccttttgt ttggcttcga taccgtaatc caccatgagg agttcaattg tttccccatc
    14821 caaggattgg ttttgtgtgg ccatgacacc catcatgaaa agtttcttaa caatttcagc
    14881 tggttcacgt ttgatacgtt ttgcgatttc cgcaacggtc ataccatctg tatattcaaa
    14941 ttctgttggc aattcatgga atttacgctc cgtaacaggt tttggagtct gattacggtt
    15001 gttcttgtta ttgccttttt tgttcttttt gttgttattc cagttactat tcttttgatt
    15061 tctcacttga ttttgactac ttcgattctt ttgttgtttt ctaggaccat cttcttcatg
    15121 atcataatcg tcacgatttt tgtctggtcg agcttgtttt ttacgacgtg tatccactgc
    15181 aggttctttt ttctcaggga cgacttcaac cactgcttga acttgctgtg cttgttcagc
    15241 taacttagcc gcttcttcaa agatttcctc tggttccttg cgtttgttag cttgagccaa
    15301 ggcttcttta gcagcctgat actgcttgaa gcgttcctca cttgaacgag cgtactctgc
    15361 attttgctct gcttttaggg ctgctgcacg ggctttaaag tcaatacgtg gagccgcttg
    15421 atttgaacgt ttgtcctctt gctgacggcg ctcatttcta cgtttttgct gaccttgctg
    15481 cttcttagct tggtcattaa agcgacgatt gtcgcggttg ctttgacctt gttttccacc
    15541 attacggcca tcgtttttct gacggtttcc gttttgttgt tggtcacggt tattgccctt
    15601 attttgcttg cgtcgttctg cctgctcttt agcacgggct tcacgctcag ccttgaaatt
    15661 acggctttgc ggtttcgctg ctactttttc tgttttcgga gcaactggct cagccggttt
    15721 tgcctcttcc ttagctacag ctggtttagc tggctcagat ttttcggttt tcttttctgc
    15781 acttactttt ggtgctgcag gttttgcttc tactttcgga gcagctgcag gcttaaagct
    15841 ggcagcaatt tttgcagcga cagcttcttc cacacttgat gagtggcttt tcacatccaa
    15901 gcccaactct tttgcacgcg ctacaacttc tttactttct tttccaagtt cttttgcgat
    15961 ttcgtacaat cttttcttag acaaatcatg tcctcctctt ctattccata agagacctca
    16021 ttttctttgt aaatccagca tctgttacag ccaaaacctt tctcgatttc ccgactgcta
    16081 tgcttaattc cagtgttgaa aacacggtta caatttctac ttgataataa tgacttttat
    16141 cttgaatctt cttggtcaga ttgggtccag catcatgagc tagaaagacc aacttggcct
    16201 tgccgtcttg aatggccttg accaccaatt cttcacccga tatgatgcgc cctgctcgct
    16261 gagcaagccc caagagatta cttatctttt gcttattcaa gtcccaactc tcttcttttc
    16321 actttgtgat ccacataagc gatcaactcg tcataaaagc tttcttccac ttccatgcta
    16381 aagctgcggt taaagacctt cttctttttc gcctctaggg cttctgcatt gtctagtttg
    16441 atataagcgc cgcggccatt ggccttgccc gtaggatcaa taaagacttg tccttccttg
    16501 ttcttgacaa tgcggagcaa atcacgctta tcaatcactt cgttagacac aacagacttg
    16561 cgcaaaggga tttttcttgt tttcatcttt ccctcctcta gcagctttta ttcttctaca
    16621 gtatcgtttt ctacttccaa ctctactgaa gcagcgtctt ccatggcttc aaattcgcta
    16681 gcagacttga tatcgatacg gtaaccagtc aagtgagccg ccaagcgcac gttttgtcca
    16741 cgacgaccaa tggcaagaga aagcttgtta tctggaacaa ccaccaaggc acgtttgctg
    16801 tcgttttcat caaagataac ttggtcaacc tcagcaggag cgatggcatt gtagataaat
    16861 tcagctggat ctgctaccca ctcgataaca tcgatatttt cttcgattgg taccatgcgg
    16921 tcatttttag catcgtaacg agctgggtgg aatttgctag taatcttctt gatattagca
    16981 ccaccacgtc caacgattgt accgatagca tccacgtttg gattgtggct acgaacagca
    17041 accttcgtac ggtcacctgc ttcacgagcc acgctcatga tttcaacagt tccatcataa
    17101 acttctggaa tttcttgctc catcaaacgt ttgatcattt ctggatggct acggctaaca
    17161 aagacgttca caccacgagg gttgtcttca accttgtaaa cataaacttc gatacgatca
    17221 tgagaagcaa aaacttctcc aggaatttgg tcttgttttg acaattgggc ttcgatgcta
    17281 ccaaggttga catagataaa gcggttgtca aagcgttcta ctgtaccaga catgatttct
    17341 tgctcatgtt ctttgtaagt attgtaagtg atggcacgtg tttgcttgcg cattttttcc
    17401 atgatggttt gtttggcaga ttgggctgct acacgaccaa actcagctgg tgcttcttca
    17461 aacttgattt tgtctccaag ttcataagct gaattaatgg caagagcatc tttcaagctg
    17521 atttccaaac ggctatcaaa tacttcatca acaacttcac ggacagtata aactgtaaag
    17581 tcacctgttt tttcgttgaa gtcaatagct acgctgtctg actgaccata gcgtctgcga
    17641 taagcggaac gaagcgactc tactactgcg tcgatgatat cttctttttt gattcccttg
    17701 tcttcttcca aaatgcggaa ggcctctagc atttctttac tcatgttttc ttcacttttg
    17761 aatcctcaaa agctatcctt tctttttcta taatttaact gctaaacgtg cttttgatac
    17821 taaactgtat ggaatttgga cggttttctt acgcgtcttg cccatatatt ccatagtcaa
    17881 ctcgtcctct tcgaaggcca acaaggttcc ttcaaagacc ttttgcttat cgatggcttg
    17941 gtagagcccg acatggatgt attttccaac cgctccagcg acggcatcct tggttttcaa
    18001 agggcgttcc aaacctggac tggtaatttc taggaaatat tgttctggga agggatctgg
    18061 cttgatggtg tctaggacag gactgataat ttctgtcaag tctgccgtgt cgttcaaggt
    18121 aattccttcg ggtttatcta caaaaatact gagaatcatg tcactgccaa tctttccata
    18181 ctcgatatcc acgagttcaa aaggagcttc tatgacaggt tctacaactt ctctgactaa
    18241 ttctacgatt gttgcgattg cgtccacctc ctcataagca agaggcgaag atatttcccc
    18301 gcctcacttt tcatattctt actatcagta tagcacgttt gccaacttat gtaaagcaaa
    18361 agcacttgta cagcctaatt ttaggctatc tcttaaaatt ctgcctcaac tcggtagata
    18421 acttgcccct tgtttgagaa tttttgctcg tattctgtca tgacattgcc ttcaaaatca
    18481 ctggcatgca aatctaacca gacaccattg agtttcatgc catattgaga aaagctcact
    18541 aaactgtact caaacaagcc acggttatcc gtcttgaaat gaatttctcc attttcaggc
    18601 aagatacgtt tgaaggtatc caagaaggtc ttgtaggtca aacgacgctt ttcatggcgt
    18661 tttttcggcc atggatctga aaagttcaga tacaagcgat caatctcacc gtcttcaaag
    18721 tagtcagtta agtcagaacc atctacccac aagagcttga tgttaggcac tccaacttca
    18781 agcaccttgt ccaaagcgta gctcaaaaca gacttttgaa tatcaatccc gatatagttg
    18841 atgtcagggt cttgcttggc catacctgaa acaaaggcac cctttccact tccaacttcc
    18901 acatgaatgg gattatcatt gccaaacaag tcccgccatt ttgccttggc ttccaaggga
    18961 ttgaggacca catactgggg atttgcctct agtaattctg ttgccccttt acgatttcta
    19021 actctcatct tctctttcca tacttgtctc ggaaatgacg caaaccatga atctcccgat
    19081 tgacattttc taaatcttgg ttcatataat acttggaaat ctgactcaaa taagacaatt
    19141 gaccatacca atacaattta cttaataccg tttgattgta cttgtaaccg tagtaggtca
    19201 accattcctt ccactgatgt tctgaaatat aatggcagag catatgggcc acatcaaaca
    19261 tgcgatcggt caagcgaacc gaatcccaat ctactaaata aatcaagcca ctatctgtct
    19321 caatccaatt actatgtcgt acatctccat ggacaatggt cgcatggtcc tctctaaatc
    19381 ctggaatagt ctgacgtaaa tcagccatca cttcactgat aaaatgattt ttacgcaaag
    19441 catctggagc cgtttcctgc caagactgta gtaaatctac aggtgtttcc atggcatagc
    19501 ccaaacgact caattgtgtc atcaacggac gtgagcgatg aagacgggtt aaaatattga
    19561 cgatttgctt acgattcata tcataggggg tcaatatctt gcctgtcaac cattcttgag
    19621 cacacatatc acgcccatct gccaaacgac gactccataa taattgtgga gcaatttgtt
    19681 ctctagctag accaggtagg attggagagg tgttcatttt tacaaagatg cgcttcccat
    19741 caggatagct acccatataa gccttgccac ttttcccagg tatgggagtc agtgttagct
    19801 cattatcacc caaatccatt tctttcctcc gtataaagtt tccttactat tctactagtt
    19861 tttggcctat tcgtcaacca ctctaacccg attctcaaag aaaagcctct ggattggctt
    19921 gggtatcatt atagcttgaa aaaggtaagc accgtcttct gcttaccttt tcataatttt
    19981 tataaataat ataagagtgg ccaactgttg taaagcatat ggactagagt agaaacccat
    20041 aggttttgac tctttttata agcaaagccc agcaacaaac ccccaagtag ataaaaaata
    20101 aacgaaggga cattagaagg tccatgcatg aaagaaaaga gagaggaagt tagcacaagc
    20161 cctaaccaag aattgtcaaa aaccgcacct tgaagaagtc ctctgaaaat gagttcctcc
    20221 tggatggggc caaaaactga ggtagtcaaa atcaaagaca gggaaattcc tctgctgact
    20281 tccgccaagt gttggacccc tgagccagaa gaaacagcga agaaatgaat ataaaccagc
    20341 gtctcaagta cacttagaag aaagattgca atgaaaagca aaagttgttt cttgcttaac
    20401 atcccaaaag aaatcatttc aaactttcta gcataagcaa cactcagcga ggttacaaga
    20461 agacttacaa gaatcaatgc agattcattt tgaaaaaaac ccccatggtt aatcgtataa
    20521 ggaactgtaa tgacaattat tagtactaaa aatccaaaac acaaggcatg aaatttatca
    20581 aaaaactcca taaaattatc ctcctcaaca aacagacttc ctacacgtca ttctttagtt
    20641 ctagcctttc caaaacaaac cattttatac tcttcgaaaa tcaaattcaa accacgtcaa
    20701 cgtcgccttg ccgtactcaa gtacagcctg cggctagttt cctagtttgc tctttgattt
    20761 tcattgagta ttagtaacca aaatccgacc acatagccag cccctatgaa tatagccatt
    20821 aaagctagca tggaatttag gaaattaaaa accaccgcag atacaaaggt tagcacaaaa
    20881 acattaaaag caatggtgtc agaagccaag actagaatat agggtgtcaa ccgatctaaa
    20941 gttttggaat ctaggaaaaa taagtgttta tacatgatga cctcctctat ggctgaaaag
    21001 caagcctttt gtttttttac cccaagaccc tatgtagaaa agtgagcaaa aacgggaagg
    21061 tcgctacaat attattgatc acatgcaccg cataggatgg ataaatgctc ttggtatagc
    21121 gggtcaaacc agcaaagatg attccaactg ttgcaaagac gaagatatct aacagactag
    21181 gcaggcttga aaaatgaggg agagcaaata aaatagaagg aagaagcaaa tcaagaccaa
    21241 atcgcgaatg cttaaagaaa gcatgttgca gtaatcctct ataaatcaat tcttccatca
    21301 gtggaaccag aaagaacagg gctatataaa tacctagctc tgcaaagtta gtcccactat
    21361 aaccaatcaa tacagcccaa ccttccgcag ttgactgaac atgtttagct gtctgaacgt
    21421 taaaagagat ctggaacact accactaata ctgtcaaaat cgaataccaa agccattttt
    21481 ttcttggaat gcggaagaga taaccatggc ctgtcttaac aagaaccaca atcatgactc
    21541 caataaaaag taaactcaag atattttgaa tccagaataa attgcctatc tgagaagaaa
    21601 attgccaata gttttggacg ataagcgtca gctgagaaag accaaatacg aaaaataagt
    21661 aagagaagac tgcacttatt ttgaatagaa gttgatactt tttcatagaa atcctcccta
    21721 ctataacctc accttgtcag gctctactgc tgtaagatta agaagacagt ttgttttttt
    21781 taaggctaac ctgactacta gataatagat acattaaggc attaaagaca atgaaaatat
    21841 gtccatagaa taaaatcaac ctcgcatcca aaccaagata aagtttgatt atcaaaaaga
    21901 tgagcaaaag aatttgaaac cataaggttt ttccaaaaat aaatttaaag cgatttcgaa
    21961 tatctacttc cttgattttt accgccaccc ctttattagc aagaaggaaa actcctgctt
    22021 caaacaaacc actgtaaaga acaagccacc caatagatac gatagagatt tgtaaaaatg
    22081 tccctaaaag aatatccaac acactactca agaaaataac aaaaaataat ctgtatttca
    22141 tattaaatac ctccattcat ttatttcact aacaatttaa tagagccttc tactcaaata
    22201 tcctgtcaga aaaggataga aagctacttt ttataatact tcaagcccca catgagcaga
    22261 agcgtgataa acaagcagag aatacaccta tataagcgat tagttgttga tagaattctg
    22321 tttctgaaat acctctatac aaacaaatga caaacataaa atctgccaag ccgataaaca
    22381 taagttgatt ggttctagga ctaaccaaat catcatttac ttatatttaa gagtatctct
    22441 tttatgttag tgtatgttaa cactaaaaaa caagttaggc caataatatt taaaatgaac
    22501 aataacgggg ttaagtctct aaaaaaatta tctactgaca ctacaagaaa tactatacat
    22561 attatagtcg aaactatctt tttcttatcc ataattattt actcctttcc taacaaatcc
    22621 aacttatcaa tcaagagcga tttttaacat aatgtagcag cacccgttgc aactttgaca
    22681 agtttagtat atcattgttt tttaaaattt ttcatccaaa tcttgaattg tcatcgaaac
    22741 ctcctaaatt gcaaaaactt cattagcccc tactttttat aatacttcaa accccaaatg
    22801 agtagaagca tgatgatcaa gcaaagaata gcaccaatat aagcgattag ttgttgataa
    22861 gattctcctg ctgtgatacc tctatacaaa caaataatag acataaagcc tgtcaagccg
    22921 ataaacatga gttgattggt tctaggacta accaaatcat cgtcttcaaa ctcttttatc
    22981 cgcatttccc tagtgaggta aacagtgacc aaaatagaag ctaagttaat aaccactaaa
    23041 agaaattgga aaactgacga aatagaaata gataagtaga aacaagcaaa ggcaactgcc
    23101 ccaggaacaa tctcacaaag aatatgattc tttttaatca agaaaatctt tcatttcttt
    23161 tctttctttt taattaaaga aaaatagatc ataaccgcaa tcatataggc tatggtataa
    23221 aatagattat accaaatact ctccctaagc ggataaagaa agatggacat gatcagatac
    23281 agaacgaaaa taatcagtat ttttttcttc ataagatttc ctcctaaatg tgcgatttat
    23341 cttagttgag caagaacatt tacactgcta gtatagcact tattttgacc ttggatcact
    23401 caaatcataa atggtcatca aaacctcttg aattgtaaaa attaaaaaaa gcaagcatga
    23461 aaaacatact ttcctcttta tgttgtattg ataccaactt gtttgtagac ttttcatcct
    23521 gctatcacat atcattttga caggcgaaac aatattaaag aaactcccct gtaaattaag
    23581 ctagcaaata caggggagaa atttattttt tagagagtac tatccgtatc ctttttggaa
    23641 gattttgaaa atatttttct aattaagtca tccatataag gaccaaatat accaactact
    23701 aaaccaataa taaaactttt aaaatccata attaccacca acatgttgct acataggcta
    23761 cacctccaag tatagctcca cctgcagcac cagttacacc tattcctata gcaaatggtc
    23821 ccaatagaaa tgtcaaaccg ttgttgcaca cccatcaatt gcgccatatg caacccctgc
    23881 tgcacaacta atttttcttc cccaatcaat atctccacct tcaacgcaag caagcatttc
    23941 attatccata acagaaaatt gtgacatcat ttttgtattc atatagtatg tcacttttca
    24001 gttacggaac aagtttaata taaaaattat caaaaaaaca taggcaataa agagaaaaat
    24061 taatttatca tagattagaa ataatatgac aaaacaattc aatgatgtta attcaatagt
    24121 cttttgtttt ttatcagaga tacttatgga tagataaata agataggttt gaaaagcgaa
    24181 gagaataata aagaatatag ccttcataaa atttagcttt catttttatg atgtagcggt
    24241 ataggctaaa tatccacaaa ccactgctcc tccaattcct cctattgcag cgccccatgg
    24301 tcctagaatt ttcccatatt tcactccacc cgctgcacaa cctaaagcag caactacagc
    24361 tgctcctccg gaattacctc cataaacctc actcagcatt gtttcattta tattacaata
    24421 agtattcata caagtctcct tttattaaaa tccacccgtt gcccctgtta ctcctgccca
    24481 aagatccaca ccaaatttag ctcctatgta tccacatgct cccataaatg gtgctccaac
    24541 accactcgca gcacaaatag ctgtccctag cccccagcca ccaaaagcag caccaccacc
    24601 ttctaagaca ttagtttgcc aattattctt gcctccttca atactagata acatagttat
    24661 atccatttca tgaaattgtt ccatcatttt tgtattcatg acaaatactc ttttttattt
    24721 ttaatttttg tcttgttgta actttgacaa gtttagtata tcatcgtttt ttaaaatttt
    24781 tcatccagat cttgaatagt catcgaaacg tcttgaatta gcttttcatt tgaaaccacc
    24841 tctaaatttt ttaaaaaaag ataatttcta attatttttt accattcagg aagtttcaat
    24901 gactattcaa gatttaataa aatatgaact tatttttatg gcataatata cctatctact
    24961 atatgaaagg aattgccaat gacttcttat aaacgtacat ttgttcctca aatagatgcg
    25021 agagactgcg gtgttgctgc cttagcctcg attgctaaat tctatggttc agatttttct
    25081 ctagctcact tgagagaact tgcaaagacc aataaagaag ggacgactgc tcttggcatt
    25141 gtaaaagccg ctgatgaaat gggctttgaa acaagacctg ttcaagcaga taaaacgctc
    25201 tttgacatga gtgatgtccc ctatccattt atcgttcacg ttaacaaaga aggaaaactc
    25261 caacattact atgttgtcta tcaaacaaag aaagactatc tgattattgg tgatcctgac
    25321 ccttctgtaa aaatcactaa aatgtcaaaa gaacgctttt tctctgaatg gactggagta
    25381 gctatttttc tagctcccaa acccagctat caaccccata aagataaaaa gaatggtcta
    25441 ctaagcttcc ttcctctgat tttcaaacaa aaatctctca ttgcttacat tgttctctca
    25501 agcttattgg tcactattat caatatcggt ggttcttatt atctccaagg aatcttagat
    25561 gaatacatcc caaatcagat gaaatcaact ttaggaatca tctcagttgg tctggttatc
    25621 acctatatcc tccaacaagt catgagcttc tccagagatt aagcattgat gtgattttat
    25681 cctatattcg ccatattttt gaacttccca tgtctttctt tgcgacacgt cgtacaggag
    25741 aaatcatttc acggttcaca gatgctaact ctattataga tgccttggct tctaccattc
    25801 tttctctttt tctggatgtt tctattctga ttcttgtagg gggcgtctta ctggcacaaa
    25861 accctaatct cttcctcctt tctcttcttt ccattcctat atacatgttc atcatctttt
    25921 cttttatgaa acctttcgaa aaaatgaacc atgatgtcat gcaaagtaat tctatggtta
    25981 gctctgccat tatcgaagat atcaacggga ttgaaactat aaagtcgctc acgagtgaag
    26041 aaaatcgcta tcaaaatata gacagcgaat ttgtagatta tttggaaaaa tcctttaagc
    26101 tcagtaaata ttctatttta caaacgagtt taaagcaggg aacaaaatta gttctgaata
    26161 tccttatcct atggtttggc gctcaattag tcatgtcaag taaaatttct atcggtcagc
    26221 tgattacctt taacacactt ttttcttact ttacaactcc tatggaaaat attatcaacc
    26281 tccaaaccaa actccaatct gcgaaggtcg ctaataaccg tttgaacgaa gtctatctag
    26341 tcgaatctga atttcaagtt caagaaaacc ctgttcattc acattttttg atgggcgata
    26401 ttgaatttga tgacctttct tataagtatg gttttggacg agatacctta acagatatta
    26461 atctcacgat taaacaagga gataaggtta gcctagttgg agttagtggt tctggtaaaa
    26521 caactttagc caaaatgatt gtcaatttct ttgaacccta caaagggcat atttccatca
    26581 atcatcagga tattaaaaac attgataaaa aagtcttgcg ccgtcatatt aattacctac
    26641 cccaacaagc ctatatcttt aatggctcta ttttggaaaa cttaaccttg ggcggtaatc
    26701 atatgattag tcaagaagat attctaaaag cttgtgaagt agctgaaatc cgtcaagaca
    26761 ttgaaagaat gcctatgggc tatcaaactc agctctctga tggagctggt ctatcaggag
    26821 gacagaagca acgaatcgct ctcgctcgtg ctcttttaac taaatctcct gttttaatac
    26881 tagatgaagc tactagcggt cttgatgtct tgactgagaa aaaagttata gataatctta
    26941 tgtccctaac tgataaaacc attctctttg tagcccatcg tctcagtata gccgaacgaa
    27001 ccaaccgtgt cattgttctt gaccagggga aaatcattga agttggtagc caccaagagt
    27061 taatgcaggc gcaaggtttc taccatcatc tattcaataa ataaggagaa tgtcatgaat
    27121 cctaatcttt ttagaagcgt cgagttttat cagagacgtt accataacta tgcgacagtg
    27181 ttaattatac ctctttcatt actatttact ttcatcttga ttttctccct tgttgccaca
    27241 aaagaaatta ctgtcacttc ccaaggagaa atcgccccta caagtgtcat ttcatctatt
    27301 cagtcaacca gtgataatcc tatccttgct aatcatttag tggcaaatca agtagttgaa
    27361 aaaggggact tactcatcaa atactctgaa acaatggaag aaagtcagaa aactgcctta
    27421 gcaactcaat tacaaagact tgagaagcaa aaagaaggac ttggaatttt caaacaaagc
    27481 ttagaaaaag cgactgatct tttttctggc gaggatgaat ttggctacca taataccttt
    27541 atgaatttta ctaaacaatc ccatgatatt gaactgggta tctcaaagac taacactgaa
    27601 gtttcaaatc aagctaatct taccaatagc agttcatcag ccatcgaaca agaaattaca
    27661 aaagttcaac aacaaattgg agaatatcaa gagctgagag atgctatcgt aaataaaaga
    27721 gctcgcttac caactggcaa tccgcaccag tcaattttaa atcgttatct tatagcctct
    27781 caagaacaaa cacaaggaac ggcagaggag ccatttttat ctcaaattaa tcaaagtatt
    27841 gcaggtctgg aatcatctat cgcaagcctc aaaattcagc aagctggtat cggaagtgta
    27901 gcaacttatg ataatagttt agcaaccaaa attgaagtac tccgcactca gtttttacaa
    27961 acagcctcac agcaacaact aaccgtggag aatcaattaa cagaattaaa agtacaacta
    28021 gatcaagcga cacagcgctt agaaaataat accttaaccg ctccaagtaa aggtatcgtt
    28081 catctgaaca gcgaatttga aggtaaaaat agaattccaa ctggtacaga aattgctcaa
    28141 atattccctg tcatcacaga tacaagagaa gtactaatca cttactacgt atcttctgac
    28201 tatctacctc tactagataa aggacaaact gtaagattaa aactggagaa gattggaaat
    28261 cacggcacca ccatcatcgg ccaacttcag acaattgatc aaactcctac cagaacagag
    28321 caaggaaatc tctttaaatt aaccgctctt gcaaaactat ctaacgagga tagtaaactc
    28381 atccaatatg gcttacaagg tcgcgtcact agtgtaacta caaagaaaac atattttgat
    28441 tatttcaaag ataaaatttt aacccattct gattaatttt cagataacac tctataacta
    28501 tttattatct tatcaaaaag gagaatcata acatggataa gaaacaaaac ctaacttcat
    28561 ttcaagaact aacaactacc gaactcaacc aaattatagg tggaggatgg tgggaagatt
    28621 tcttatatag atttaatata attgaacaaa aaaatacaaa aggatttcat cagccaatac
    28681 aactataaaa aaacaagacc aagagaacgc actctcggtc ttattttttc ttcattctat
    28741 atgtattaca gtaagtacct gacgaaaaac gtgatcttgg caggtggtat tgagactggt
    28801 attgggatga ctttccacaa ttttcataac ggtatagaga ccaactcctc tctcctcccc
    28861 tttagaactt gctccaaagg agaagatttc agaaatatcg attccctctt ctttgatgga
    28921 attttcaatg ataaaggtct cctgtgctcc atttttaaaa aaggcaattg aaacatgagg
    28981 ttgacaggct tctacactag cttcaatagc gttgtcacaa aggatagaca caacggttag
    29041 aaaatcaagt agactcatcc cctctacctg aatctcctct ggaacttcga cattaaagac
    29101 aatgttctta tctctagctt ttataaattt tccagctagg agacttttga gggcacgatc
    29161 ccgaacattc accaatcggc caaggtcata tttattgtcc tgcaatttct gactggaatc
    29221 ccttaagacc gagtcgtaga tctcttttat ctgctccata tcctcctctt caatgcccaa
    29281 acgtaagctg gttaagaggt tagtgtagtc atggcgaaaa ctccgaattt ccttgtaaag
    29341 ttcctctata tgccgactat agcgttccat atctctgtag cgcaaggtct gctcttggtt
    29401 cagttcctcc tgaagttttt ccttcaaata ggtatccaat ttcttgatac cccccataaa
    29461 aaagagtagg taaaacacta ggatgagatg gcgaacagtc gttgattgaa taccttgttc
    29521 atattcaagg taagatagac tttgcatcac tagataataa gcccccattg cccagttaat
    29581 cttagtaaga gacttttgaa aacctgtatc tagaaactcc cttctcaatc tagtgaaatc
    29641 atagtctaac catttcaaaa agactaaaac tatgaagcaa acgaatatca taatcaataa
    29701 aaagatagga ttgccatccc catctacaat tccttgcccc aaaaatggaa gcacaaaata
    29761 ggaaacacct ctgtaaaaga gctctactaa tatcattggg aagagaccat aaaaaataag
    29821 gattttttta ggaagccctc ttaacaataa gaaagataga cctataccga acaaaggttc
    29881 tataaaatag gacaaatatt tacttacagc caaaaattga aaagtcgtaa aaatgatccc
    29941 tagaagaaat ttcagaagaa aggccttaaa aatcctctcg aaagtaagat caattccatc
    30001 caccttaaag aagatgacaa tttctagtcc attagtaaca agtgtataca acaatatcca
    30061 agcaatgttc ataaattctc ctagctcagt gtaatttatt gatggcctca gacacttccc
    30121 tgaccttata acgggcgatt agacaacttc caccattggg aaagaaaagc agtttttctt
    30181 tcttatccaa atgcaccaca tttgcaggat tgatgagaaa agagcggtgg cactgcaaga
    30241 gacggggctc ctgcttgaaa acctcctcta aactcgctgt aaattccagc ctgtctgtct
    30301 tggtatagag aataacacga tgggctctgg gcgacgtttc gagatagtaa acctctttaa
    30361 aaggatactg aaattgggca aattttgatt taaagtaaaa gcaatcttcc gccagacttt
    30421 tactatcttg actattggca tagaggaggg ctgtctcgat ccgagattca aactcctctg
    30481 ctgacaaggc cttatcaatg tagtccaaag cagacacttg gtagcgaaaa gacaggggca
    30541 taaactccga gtgagtcgtc acaaagacaa tcagggcata aggatcccga tcccgaatct
    30601 ttctagccac ttccagtccc ttcatctctt catttcgaat ctcaatatcc aaaaagaata
    30661 gctgatgggc ccccttctca tgcacttcag ccagcagttg gtccggcttg ccaaatacct
    30721 caaaagagct aggaatgata tgatgtgctt tcaaaagttt ctcaatcgtc gtttcaattc
    30781 tagtctgttg ggaaaaatca tcctctaaaa caaatattct catcttctta ctctccctct
    30841 ttcaaccatt tttgacgaat ttctctatag cgacgtctgg catacttgac agcgtattga
    30901 ccttcttctc cgagaaaaag gattctttct tgaaaatcaa tcgattttaa tttatcaaga
    30961 ttaaccaaac aatttcgatg acatctcatc aaggtttccc cacattggtt ctcaagatta
    31021 cttaaatttt gaagcatatt aaaactagct tcttctgtaa caatctgtac ggtatgggct
    31081 ttagttggat gtgtttggat atagtagata tcgtcgatgt ttactttata gactgttttc
    31141 tgcgtctgaa taatcatata tttcattgga ttctcctcct aagaaaagaa taacagaaaa
    31201 ggtctagccc aagcacaccc aatcctaaac agtctttgaa aactcctaaa aggattattt
    31261 tctacaacat catgatgagc ttacaactat tttatcataa aattcactca gcgcaattca
    31321 agacatttca atgacaatta aagatttgaa taaaaaaaaa gaattaaaag tgataaacta
    31381 acagtatcaa aacacagttg ccagaagtaa gactggcacc cagattaaag gaggcattcc
    31441 catgacagat acagacccca tcaaaagagc tcatactttg attactgact taaacaaagc
    31501 ctaccaagca tgcaaacagg caagcgctga cgacgtccgc tttcaggagc aattaaactc
    31561 tattcttggt tttctagcca aggctgaaac agtggataat cgattcttga ttgaactaga
    31621 aaaattttac cagacttcca gtcttctcat gggactcagc gctcttgatc cagatgctcc
    31681 aactcgcgcc gcttggcggg cctatgaccg cttccacttt gaccaagtca agaccaagtt
    31741 aatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaagctgt acttgagtac
    31801 ggtaagacga cgctgacgtg gtttgaagag attttcgaag agtataagtc tctacggacc
    31861 aaccattatc ctgtagtaac aaaaaagaag ttgagacaga ctgaactcaa cttctttttt
    31921 agtgtcgtat cagcaatgtt agtcctgcat ctgacgtttt acctgataag gcaaatacaa
    31981 gactagtaaa accaaaccag ctcctagtaa ggctagaagg gctagttttt cttggaaggt
    32041 aatcaaccca acaactaatt ccacaagtaa aacaaaactg gtcaatcctc gaactacctc
    32101 ctgtaagcct ttttcctttt gccccttgtc cagcggaaag agttgggtca aatactggta
    32161 gtcaaaggca tgatagaggg ccagcaactg gaagagcaag aggtagttaa agagaactac
    32221 cactgctgtc gcaatccaag cttgctcgat aaaaacctgc gccagtacgg aaagtaagag
    32281 aagacgaaga ctgagagcaa agaggtcgcc atttcgcaga taagaacgca gatagagatt
    32341 ttgccaaatc ttcccaggca ccttctgaac agcctttaag ataaagtcca gataggcacg
    32401 acgcttaacg ctgtttgaaa ttcccttgac ctgcgtaaag agggcaaaga aacgaagcaa
    32461 gacttgctta cgcttgcttt cttgggaaat gacatagtcc cagtccagcc cagtttcagt
    32521 gaaaaatttg ctggcctttt gacgaaagtg gaaatatttt cctaccccca ataaaagcac
    32581 atagagcaga aaaactggca agccataacc cattgctaaa aataaaggcg caaataacag
    32641 caagaaaagg gtctgtacaa agagccaaaa gactagggaa atgccagttt gacgcttgag
    32701 atggagcttg atttcctctt ctccaactaa gagaaagagc ttgtctggag cctccatata
    32761 ggtggcaatt cctccccaaa gtaaaagtaa aacagacgta attcctacaa acaaaaggat
    32821 aggccaatga ttttcaggaa aatgttgtaa gagttgactg tactggtagg ctagaaagcc
    32881 caacaggaca agcaggaaca agacaaagtg gtcattgagc acatagcgca gataaccaag
    32941 acactcctta cgaaaggcct gctttctctt taaaaacaag tctttcatag atcctcctct
    33001 ttggtcagag ccaagtaaat atcattcaaa ctagcctcag gcatatcaaa ggcttcacgt
    33061 agttgcagga gattgccttt ggcacgcacc tctcccttgt gaagaatgac aaaggcatca
    33121 cacatcttct ccgccgaatc cagcacgtgg gtactcatga gaatagactt gcccttttgc
    33181 ttctccactt ccaaaagctg aatcaaatca gaaatagcca gcggatcaag accaaggaaa
    33241 ggctcatcca cgatgaaaag acttggatcc accacaaaag cacagataat catgaccttc
    33301 tgcttcatcc cttttgaaaa atgaacaggg aaccagtcta atttctgttc caaacggaac
    33361 atttttaaca agggctctac tcgttcgaaa gccacttttt gctcaatacc gtaagccata
    33421 gcaaccgttt cgatatgctc tctgagggtc aattcctcat acagactagg cgtctcagga
    33481 atgtagccaa tctgcttgcg gtagctagtc gcatctcctt gcagagtcag gccattgata
    33541 ttgatggagc cactataagg tgccaacaga ccgataatct cattgatcgt cgttgatttc
    33601 ccagcaccat tgagaccaat caaaccgacc aactgcccac tttcaacagt aaaggacaca
    33661 tctttcaaaa caggaacatg aacatagcca cctgtcaggt ttttaatttc taacatattt
    33721 tctccaaatc tggtataatg tagctatatt atatcaaaat tcagtacagt agaggtagat
    33781 tttatgtcag attgcatttt ttgtaaaatc atcgcagggg aaattcctgc ttcaaaagta
    33841 tatgaagatg agcaggtcct tgcctttctt gatatctctc aagtaacact aggacacacc
    33901 ttggtcgtgc caaaagaaca ctatcgcaat cttttggaga tggatgctac gagcgccagc
    33961 caactctttg cccaagtacc aaaagtagct caaaaagtca tgaaagtcac taaggctgct
    34021 ggtatgaata tcatttccaa ctgtgaagaa gtcgctggtc aaacagtttt tcatactcac
    34081 gttcaccttg tgcctcgcta cagtgctgac gatgacctca agattgattt tatcgcccac
    34141 gaaccagact ttgacaaact tgctcaagtc gctgaaacca tcaaaaacgc ttaaggagtt
    34201 gctatgaaat tatccaacct actgctattt gcaggagctg cagccggaag ttatctggtt
    34261 acaaaaaatc gccaaaccat cacagatgaa gtcttgaata ccactgaccg cgttcaagct
    34321 atcaaggacg atgtggatat tatccaaaac agcctgcaaa tcattaacca gcaaaaagaa
    34381 cttatcaagg aataccaaga agacttgact tacaagttta aggtcttgga aaaggatatc
    34441 cagactagac tagctgtgat aaaagaaatg cagggaactg aagataagta aaaagagccc
    34501 gacggctctt ttttaaaacg taaacaagtt atagtgaatt gaatttagaa tagcacatca
    34561 tagtttctaa agcattgtta gagattactt taaaatcctc ttatcaattt gttcatattc
    34621 tatttcaatc tactatatat atcccatcaa ctatgcatca taatttaggt aactcatact
    34681 caataaaaat caaaaagcaa actagaaagc taggcacaga ctgctcaaaa caccgtttta
    34741 aggttgtgga tagaactgac gaagtcagct caaaacactg ttttgaggtt gtggatagaa
    34801 ctgacgaagt cagtaaccat acccatgaca agccgacgct gacgtggttt gaagaaattt
    34861 ttgaagagta tcattcacca tttcacaact aaatcgaaga ctttctgccg taatgaaaac
    34921 acctgaaaca gcttggtttc agctgtccgg aaactttaag acttaggttc aaagtttagg
    34981 tatggaactt cgaaggaggt cgctaccgtc cgtcattact taggaaagtc ttaaaaaatc
    35041 tataaagtaa aaagagcccg acagctcttt ttgtttatac tcaatgaaaa tcaaaaagca
    35101 aactaggaaa ctagccacgg gctgctcaaa acactgtttt gaggttgtag atacgactca
    35161 catatatacg gcaaggcaac gctgacgtgg tttgaagaga ttttcgaaga gtattattct
    35221 ccatcaaagg catcttttat atggtcaaag aagccttttt tctttggatt tactttcaag
    35281 tcaccagcag tcgcgaattc tttcaaggct actttttggc ggtcgttcaa gcctgtcggt
    35341 gttacgacat taacagtaac gtattggtca ccaactgcac cgccacgaag gctcggtgcc
    35401 cccttactac gtaggcggaa tttcttacca gtctgagttc cctctggaat aaccaattca
    35461 acatcaccgt gaacagttgg aatatctact gtatcaccaa gagccgcttg gacaaagttg
    35521 aggttgagat tgtagaagat agtcgttcct tcacgttcaa acttgtcgct agcttctaca
    35581 gaaactacta catacaagtc accataaggt ccaccgttaa agcctgcttc accttgacca
    35641 gcgaggcgaa tttgttgacc tgtttccaca ccagcaggga ttttcacatg tacgctatga
    35701 gcttgtttct catgacctgt tccatgacag gttgtacatg gatatttgat ttcttttcct
    35761 cgaccgtgac agacatcaca ggttacttgg cgacgcatca taccaagagg agtctgcgta
    35821 tcgacgttaa tgacaccagc gccatgacag cgtccacaag tgactggact tgtccctggc
    35881 ttagcaccag agccattaca tgtacgacag ccagcttcac gatgatactt aacttccttc
    35941 tcagttccga agatagcttc ttcaaaggtc aaattgacac gatactggag atcatctcct
    36001 tggcgaggag cgtttggatt gcgcgaagaa ccgcctccgc cgaagaaact tgagaaaata
    36061 tcctcaaaac caccgaagcc acctgcccca ttgaaaccac cgaaaccacc agctccacca
    36121 aaaccaccat tggcgcctgc agcaccatac tggtcatagg cagcacgttt ttggtcgtca
    36181 ctcaaagtct cataggcttc ttgaacttcc ttgtacttgt cctcagcacc aggctccttg
    36241 ttgatatctg ggtgatattt tttggaaagc ttacgataag cctttttgat ttcgtctgcc
    36301 gaagcgtttt tggatacccc cagacgatca taaaattcag tattgttcat acaagatacc
    36361 aagaccgtaa aattcgactg aaaaatagga aatctgacgc tggagcgacg ctcctagaca
    36421 gatttctctt ttttccgaga atttaggtcg ggttcaattc ctttctttta tattgatgag
    36481 ataatactcc agccactcgg ccctatccct ttttaccgaa aaacagaggc tgggttgcaa
    36541 cctctgtggt tagattattc atttgaaata aaaatggttg tattatctct agtagtaatc
    36601 ttcaaagacc ttttagctaa atcaaactca aatttattat agtgtgggtc ttgtgtttta
    36661 tataggtgta actcctgaca tatatgatcc gaaaagtaaa tcataacatc tttaaacaca
    36721 gaagtgtggt catatttact cttaccaaca gctacttctg taaattcttt ataaatgaca
    36781 tttccatttc tttccacagt caccagcatt tctgttggat tataatccaa attaaataat
    36841 tattcaaaaa aactttatct tttcatgata atctgcaatg tcagaaagat tatttttatt
    36901 aacactcata acactagccc cttatttttc agtaaactct ccgtctacga cgtcatcgcc
    36961 tgcgtttcct gttgcttgtg cgccttctgc tccttcttga gcttgttgcg ctgctgcggc
    37021 ttgttcgtag agtttcacag caaggccttg agctttttcg ttcaacgctt caagtttcgc
    37081 tttcatgtcg tccaagttgt tgtcttcttg agctttctta aggtcatcaa gggcagcttg
    37141 ggcagcgtca cgttctgcgt cgaagccttt accttcagtt tccttgattg tcttttcagt
    37201 cgcaaagatt gcttggtcta cttcattacg aaggtctact tcttctttac gtttcttatc
    37261 ggcttcagcg tttgcttctg catctttcat catgcggtcg atttcttcgt cagtcaaacc
    37321 tgagttcgat tggatgacaa tagtttgttc tttttgagtt ccaaggtctt tggccttaac
    37381 agacacgata ccgttcttgt cgatgtcaaa tgttacttcg atttgaggaa ttccacgagg
    37441 tgcagctggg atatcagtca attggaagcg tccaagagtc ttgttatctg ctgccattgg
    37501 gcgttcacct tgaagaacgt ggatatcaac ggctggttgg ttgtctgctg ctgttgagaa
    37561 gacttgtgat ttagatgttg ggattgtagt gttgcgatcg ataagttttg taaatactcc
    37621 acccattgtt tcgataccaa gtgacaatgg cgttacatca agaaggacaa cgtccttgac
    37681 atcaccagta atcacaccac cttggatagc cgcacccata gcaactactt catcagggtt
    37741 tactgatttg tttggttctt taccagtttc agctttaaca gcttcaacaa cggcagggat
    37801 acgagttgaa ccaccaacaa ggataacttc gtcgatttct gacaagctca aacctgcatc
    37861 tgaaagggct tgacgaactg gaacttttgt acgttcaaca aggtcacgag tcaaatcatc
    37921 aaatttcgca cgagtcaaag tcatttccaa gtgaagaggt ccagcctcac ctgcagtgat
    37981 aaatggcaag ctgatttgtg ttgaagttac accagaaagg tctttcttcg ctttttcagc
    38041 cgcatctttc aaacgttgca ttgccatctt gtcagtagac aagtcgatac cgttttcttt
    38101 cttgaattct gctaccaagt ggtcaatgat tttttggtca aagtcgtcac caccaagttt
    38161 gttgtcccct gcagttgaca atacgtcgaa gacaccgtca cccaattcaa ggatagagac
    38221 gtcgaatgta ccaccaccaa ggtcaaatac caagattttt tcttctttgt cagtcttgtc
    38281 caaaccataa gcaagagctg ctgcagttgg ttcgttaaca atacgttcta cttcaagacc
    38341 agcaatttta ccagcatctt ttgttgcttg acgttgagcg tcgttgaagt aagccggaac
    38401 tgtgataaca gctttggtta ctttctcacc aaggtagtct tcagcgtagc ctttcaagta
    38461 ttgaaggatc atagctgaga tttcttgtgg agtgtattct tttccatttg cagaaacttt
    38521 ttcagaagtt cccatcttag atttgataga gataactgta tctgggtttg tgactgcttg
    38581 acgttttgca gcatcaccaa cgatgatttc tccgtttttg aatgagacta cagatggagt
    38641 tgtgcggttt ccttctgggt ttgcgatgat tttgctttca gttccttcaa gaactgcaac
    38701 tgctgagttt gttgtaccta agtcaatacc gataatttta gacatgtgtt tttctccttg
    38761 ttagttaatt ttctattttt ctttttgata ctcttcttaa gtggcgacgc cgtcagagct
    38821 tgacttcgtc aatctctttg actaaacttt gagcctaagg tctcaaaatt tgcgcaaata
    38881 gcaccacggc gaagagtatc gtctttggtt cgcttcgctc actattacaa agccaaacat
    38941 attttatctt tcttcatagt ttattgtcgt ttcggacaag ttttctatta tgtaaattgc
    39001 gacacgagga gtcgaaatcg attttatttc gacgacgagt tagtaaggaa gctaggcaaa
    39061 cgtcatagcg attgccgttt tctgacgagt tgctttagct accgtcagaa ttgccgaatc
    39121 gaacacgccc taagctctga gtaaaaaaga tagattttct tgtgttcatc gaacacttcg
    39181 tcaatctcct atttttactg cgagcttttt acgagctttg tatcttagtt atacaccact
    39241 accattgctg ggcgtaggat gcggtcatgg agtttgtagc ctttttgaaa gacttgggcg
    39301 atggtatcta ctgggtgttc atcgtctgct gggagagttt ggatggccat atggtagtta
    39361 tggtcaaatt cgccatctgc tgcgatttct tcaattcctt cttctttcaa agcgtgaatc
    39421 aagctttctt gcaccatcgc caagcccttc ttcacatcat ctgtcaaacc ttcaactgca
    39481 agtgcacgct caaggttgtc aagagatggt aagattgctt ttgccaagtc ctggctacga
    39541 taacgttgca agttttgacg ttcttcattg gcacggcgtt ggatattttg catttctgca
    39601 tgagcgcgaa gatatttgtt ttcgaactca tctgcacgtt catttgccaa gtccaactca
    39661 gacttttcag gagttgtttc ttcagctgtt tccacaactt cctcttcttg aacttcttct
    39721 acttcttcat tttttatatc ttgggccatg ttcgcctcct ttaatgattt caatcttaat
    39781 gtacttcgta atgattactg ctgaggtagc ggtaaaaatc tgtcaacttc atggtcaaaa
    39841 cacggttgac cacattgact tgattgatta gctgttggta atccagatta actggaccga
    39901 taatggctag aattccaact cctcgataag gaatgaggaa cttactacta atcaccgcta
    39961 ggtcagctaa acaggactct tgaccgtctg caacacgaac attttgcatc tgatcctcac
    40021 gcaacccctc acgaatctcc aaggccacct tttgcggttg gtcaaagaac tgataggctt
    40081 ctagattggc aaaattcaag agattgacct tgcccgccat cacaatgttt tcgttgaaca
    40141 tttccttaaa gatgtgttca aagagatcga tgacattatc cgttgttgta aagtaacgct
    40201 ggataatctg cggaatctcc gtccgaatct tgtagtgaat atctaaaacg gtgtgaccga
    40261 ggaaacgttc ctgaatgatg ctcttcagtt tcagcaaatc ctcctgcaag aagttccttg
    40321 gaatcagaaa ctgactagta accgttcgcg actcgtctag ggtaaatacc gccaaggctg
    40381 tatgttgccc caaaacaacg atatcaaagg ctgtcaaacg ttgcctgctc ggctcaacat
    40441 ccagtgccac taccgtacag ccactcaggt ctgttagcaa gttagcagcc tcttgcagaa
    40501 tatcctccaa tttgaagaat tcctgatcaa aggctttgac aatctcatat acctcatttt
    40561 cagccagccg gtgaaaatcc agtgagtgtt tcacatagta ctgaaaacca gcaacacttg
    40621 gcatccgacc gcttgaagta tgagccttct caagcaaccc ttgtttttct agttccgcca
    40681 tgtcattacg aatggttgca ctgctagagt taatagactc ttgcaaggct tttgatccga
    40741 caggttcgtg cgttttggta aagatgtcaa taatcagatt taaaatatcc tgctgacgct
    40801 ctgtaaccat ctcatcactc ctctctgtct gatcaattag cactcaatgc atccaagtgc
    40861 taacttatga ttctattata cacccttaaa agagaatgtc aagacaaaaa ctcaaaaaat
    40921 tagcactctt ttatcaagag tgctaaaaat caatctgaag acctagaacc ttgctattca
    40981 tctactcgat atttcctttt tagtctgaaa taaccatgaa ttagataaga aatcagaaaa
    41041 gcatataaaa caaaaataag aaagaaagac atagaaagat cctctctaaa caaactcgta
    41101 caaacaacaa gaccacctaa gaaagtagtt gtacaggaac gaagtctaga tcgcataact
    41161 tcccatttga gccccttact catatagact tgatcctctg gaagtaaatc agaagacgat
    41221 ttacgaagaa tcgaacaaga acctgctcct atcaattccc aacctctatc tctaaaatct
    41281 tgcagttcat gcttatattt tttaagaaat ctagaatcat agatacggta gatgacatcg
    41341 tctggttgac attggtcaaa atagaacaaa ccaaaacgac tcgttctata cctccaacct
    41401 ttcaaatgca tctcatgtaa atattcttct tacttgtcca aatcaacaat ggtgaaaatc
    41461 cgaaattcta ctctgctatt cattgtctta ccccaaaatt agaaaacatg cctggcgtta
    41521 tttattagat aattctttcc acttttgact caatctccaa aaaatataag aaatctgaat
    41581 cgcaaaaact atcaataaaa cccaatctat tatgaaaatc aaaaacactt tccaactgaa
    41641 agaactacct ccagtgacaa actttgagaa aaacggtagt agagctaaaa agagaagtaa
    41701 aataggaagc atccgcattg ttaaaatccg tttggcataa aaaaatcttt atttaaacta
    41761 aaatattata tcaaaattta cgccagtttt tgaacggctg atgtagatat tttatacttt
    41821 caaaatgttt aaatgtgatt atttattttt gaaaaatagc tcaccagccc gactgaaagt
    41881 gcttatagaa tgataataag tcgcctgccg aaaacagcgg aaaatagcgg tgttatgcgg
    41941 agataatctg acgcgatgcg aaagtatatt gcatacttat tttcaacaat ttagcagagt
    42001 atttttataa gtgtgatata atagaagtat aatttgttct gatagtttat tttatggaga
    42061 agtagatttt tagaatgcgg agggttcaat atggttgagt ttataaagtc taagaaagaa
    42121 atgagtgagg aggatattaa agcaaatttc atcactcctg ctattgtatc caaaggatgg
    42181 aaaaatggtg agcatatcgc ttacgaagaa tacttcactg atggtcgaat tgaggttaga
    42241 ggagataagg ctcgtcgtaa agaaggaaaa aaatcagact attcactgta ttaccaattt
    42301 ggaactcgaa ttgcaattgt tgaggcaaag gataataaac acagcgttcg agcaggatta
    42361 caacaagcta ttgaatatgg agagatttta gatgttccat ttgtttattc ttcgaatggt
    42421 gatggcttta ttgaacacga ccgtatcacg agagaagaac gtgagctgga gttagacgaa
    42481 ttccctactc gtgaagaatt attttctcgt atgacgaagg aaaaaggatt gacgtacgaa
    42541 attacagaag ctatctcaac tccatactat acagacgcct tctcaatgaa aacgccacgc
    42601 tattatcagc aaatagctat caaccgtact attgaaacag ttgccagagg acaaaaacga
    42661 gtaatgtttg tgatggcaac aggaacgggg aaaacgttca tggcttttca aattattcat
    42721 cgccttcgaa aagctggttt ggctaaacga gttttattct tagcggatag aaacatctta
    42781 gtagaccaaa cgatggctga agactttagg ccattcgaaa aggtaatgac gaaaattaca
    42841 ccaaaacttt tgactgctcc tgaaaaatta aattcttttg aaatttatct agggctttat
    42901 cagcaactaa ctggtgaaga tggaactgaa acacattatc aaaaatttga caaagacttc
    42961 tttgatttaa tcgtaattga tgaagcgcac cgtggttcag ctaaggaaaa cagtaactgg
    43021 cgtaagataa ttgattattt cagttctgcg acacagattg ggatgaccgc tactcctaaa
    43081 gaaaccaaga atgcttccaa tacggaatac tttggtgagc caatctatac ttatagttta
    43141 aaacagggaa tcgaggatgg ttttttggct ccatatcgtg ttatgagagt taatttagat
    43201 gtagatgtgg atggttatcg tccagaaact ggaaaagttg atgctaacgg acaattaata
    43261 gaagataggt actacggcag gaaagatttt gataaaacca ttgtcattga tgatagaacg
    43321 caaagagttg ccaagtttgt ttctgattat atgaagcaaa acaatgcacg atttgataaa
    43381 acaattgttt tttgtgttga tattgaccat gccgagcgaa tgcgtgctgc acttgtaaaa
    43441 gagaatctag acttagtcca agaagactat cgttatgtca tgcaagtaac tggtgacaac
    43501 gctgaaggaa aagctcaact ggataacttt atggatgtca attctaattt tcccgctatt
    43561 gtaacaacgt ctaaattatt aacgacagga gttaatgcta aaacatgtcg tttgattgtt
    43621 ttagactcta atatccaatc catgactgaa tttaaacaaa ttattggtcg tggcacacgt
    43681 ctttatcctc aaaaggggaa agaatttttt acgattattg attttcgaaa tgttaccaat
    43741 ttgtttgctg accctgattt tgatggtgat ccagtgaagg tgctggaaac aggtgcgaaa
    43801 acagtcagtg gttctacgcc cggtttcgta agtgaggaag gtgacccagt agaaaaatat
    43861 atcgttacag acaagcaggt taccattctt aattctactg ttcaagtatt ggatgaaaac
    43921 gggaaactga ttaccgaaag cctgaccgac tacactcgga agaatatctt aggtagctac
    43981 gccactttga acgattttat cacagtttgg catacggcgg ataaaaagaa gcttatctta
    44041 gacgaacttt ataaaaaagg agtttatcta gatgctattc gagagtcgga gggaatatca
    44101 gaacaagaaa tcgatgattt tgatttactc ctaaaacttg cctatggtca aaaagaatta
    44161 accaaaacgg aacgtatcaa taagctcaaa caaagcggat atttatataa atatagtgag
    44221 gaagcgcgtg ctgttttgga aattttactg aacaaataca tggataaagg tattggagaa
    44281 ctcgaaagca ttgaaacatt aaaacttcca gaatttcaga tatatggtgg aaccttcaaa
    44341 atcatcaata cttattttgg agataaaaaa cgatatttac aagcaattaa agaattggag
    44401 caagagctat ttacagtagc ttaatgaaag gaaagtatgt caattacatc atttgtaaaa
    44461 agaattcaag atatcactcg aaacgatgct ggtgttaatg gtgatgctca acgtattgag
    44521 caaatgtctt ggttattatt cttaaaaatt tatgatagcc gtgaaatggt ttgggaatta
    44581 gaagaagacg agtatgagtc aattatccca gaggaattaa aatggcgaaa ttgggctcat
    44641 gctcaaaatg gggaacgggt attgacaggc gatgaattac ttgattttgt caataacaag
    44701 ttattcaaag agttgaaaga gcttgaaata acttcaaata tgcctattcg aaaaacgatt
    44761 gttaaatcag cttttaaaga tgcgaacaac tatatgaaaa atggcgtctt gttacgccaa
    44821 gtcatcaatg ttattgatga agttgatttc aatagccctg aagatcgtca ttcgtttaat
    44881 gatatttacg aaaaaattct taaagatatt caaaatgctg ggaactcagg agaattttat
    44941 acaccacgtg cagcgactga ttttattgcc gaagttcttg acccaaaact tggagaatca
    45001 atggcagacc ttgcttgcgg aacaggaggc ttcttgactt cgactctgaa ccgtttaagt
    45061 agtcaacgta aaactagtga agataccaaa aaatataata cagctgtttt tggtattgaa
    45121 aagaaagcat ttcctcatct tttagcagtt acaaatctgt ttcttcacga aattgatgac
    45181 cctaaaattg ttcatggaaa tactttagag aaaaatgttc gtgaatatac ggatgatgaa
    45241 aaatttgaca ttattatgat gaatccacct tttggagggt cagaattaga aacaataaaa
    45301 aataactttc cagcagaatt acggagttct gaaacagctg atttatttat ggctgtcatt
    45361 atgtatcgtt tgaaagaaaa tggtcgtgtt ggagttattt tacctgatgg ttttctattt
    45421 ggtgaaggtg taaaaactcg cttgaaacaa aaactggtag atgagttcaa cttgcatacg
    45481 attattaggt tgcctcatag tgtctttgca ccgtatacag gaatccatac gaacattctt
    45541 ttctttgata aaacaaagaa aacagaagaa acttggtttt atcgtttaga tatgccagat
    45601 ggttataaaa atttctcgaa aactaagccg atgaagtcag aacacttcaa tcctgttcgt
    45661 gactggtggg aaaatcgtga agagattctg gaaggtaagt tctacaaatc taaatcattt
    45721 acacctagtg aattggctga gttgaattat aatttagacc agtgtggttt tccaaaagag
    45781 gaagaggaaa tcttaaatcc ctttgagttg attcagaatt atcaagcgga aagagcaact
    45841 ttaaatcata agattgataa tgtattagct gatattttgc agttgttgga ggacaaataa
    45901 tgacaccaga acaacttaag gcaagtattc tccaaagagc gatggaaggg aaattagtgc
    45961 cgcaaaatcc caatgacgaa cctgcaagtg aattattaaa gagaattaaa gctgaaaaag
    46021 aaaaacttat cagtgaagga aaaatcaaac gagataaaaa ggaaactgag atatttcgtg
    46081 gtgatgatgg gaaacattat gggaagtttg ctgatggaag tactcaagaa attgatgttc
    46141 cttatgatat tcctgatact tgggagtggg tgaggttttc tacattggtt gaaattgtca
    46201 gaggtggctc tccacgacca atcaaagatt atcttacttc tgaagtagat ggaataaatt
    46261 ggataaaaat aggtgatact gaaaagggtg aaaagtatat aaataatgtt aaagaaaaaa
    46321 tcaaaaaatc agggcttaac aaaactagat ttgtaaaaaa aggtacattt ttgttaacta
    46381 attctatgag ttttggtaga ccttatattt tgaatgttga tggtgcaata cacgatggat
    46441 ggttggctat ttcgaactat gaaaactcat taaataaaga ttacctattc tatattcttt
    46501 catcaaatgt agtttattct caatttctat ctctaattag tggagctgtt gtgaaaaact
    46561 tgaatagtga taaagttgct tctattctta tccctctccc cccactatcc gaacaacaac
    46621 gcataataga agcaatcgaa tcagctttag aaaaagtaga tgaatatgct gaaagttata
    46681 atagactaga acagctagat aaagaatttc cagataaact aaaaaaatct attcttcaat
    46741 atgctatgca aggaaaatta gttgaacaag acccaaatga tgaatcagtc gaagttttac
    46801 ttgaaaaaat acgagcagaa aaacaaaaac tctttgaaga aggcaagatt aaaaagaaag
    46861 atttggacat ttctattgtt tcccaaggag atgataactc ttattatgag gaagtacctt
    46921 gtgaaatacc agaaagttgg gaatgggtga ggttaaacga tataacctcc tacattcaaa
    46981 gagggaagtc tccaaaatat tcaaatattc ctatataccc tgtgattgct cagaaatgta
    47041 atcaatggtc tggcttttct attgacttag caagatttat tgatcccaaa acagttcata
    47101 gttaccagaa agaaagatta ctaagagatg gtgatttaat gtggaattca acaggtctag
    47161 gtacgttagg acgattagct atttaccatg aaaataaaaa tccttatggc tgggctgttg
    47221 cagatagcca tgttacagtt ataagagtct tatcgggggt tattaattgc catttcatct
    47281 ataattttct ttcctctcct attgttcagt cagttattga agaaaaagcg tcgggtagta
    47341 caaaacaaaa agaattatta acgaaaacta taaaagaata tttaatcccc ctcccacccc
    47401 ttcctgaaca atccagaatc gttgataaaa tcgaacaatt tttcgcccat attgacgcac
    47461 taatttagta taatagtttt acgtgcggtg gaatttctat caaacataag ataggagttt
    47521 tacgttgaaa gattttgaat tacatttgaa gaaagctggt ttagctgaaa atactgtgcg
    47581 ttcgtacctt tatggtgttc gatttttctt ggagaattac gaactcaaaa tggaagattt
    47641 atttgaatac aaaggatatt tattagacaa ttttaaacct aaaactgtca atttgcgact
    47701 acaaggagta aataaatatc tggcttttat tggtcatgat gatttgaaat taaaatttgt
    47761 caaagtacag caaaaaccgt ttttagaaga tgtcattagc catgctgatt acctttttct
    47821 taaacgtagt ttgaaaaaag atggtatttt aaaatggcat tttgtcgttt ggtttttagg
    47881 tgcaactggg gctcgtgtaa gtgaacttat taaactaaaa gtagagcatg tagaaatcgg
    47941 ttattttgac atttactcaa aaggtggtaa aattcgtaga ctgtacattc ctaaaaaatt
    48001 aagaaatagt tgtcttagtt ggttagaatc agaaaatcgc cgaagtggtt atttattttt
    48061 gaataaattt aacgaaccaa ttacagcaag aggagttgct caacagttaa aaaattatgc
    48121 tgataaatac aaaatgaatc ctaaagtaat ttaccctcat tcttttaggc atttatttgc
    48181 taagaatttt ttagcgaagt ataatgatat tgccttgctt gcagatttga tgggacacga
    48241 aagtatagaa actactcgaa tttatctaag gaaaacagct actgaacaac aaaatattgt
    48301 agataaaatt gttaattggt aaaaaataac aggtggtcaa actgactacc tgctattttt
    48361 gtgattatgg ctcttattat gggaacaaag acgaaacaac ctcttatcct atatatgaaa
    48421 tcccagaagc atggagatat attaaatttg ctagcctagt taacttcaga ataggaaaaa
    48481 ctcctccacg cagtgaagct actttttggg gaactgaaat accttgggta tctatatcag
    48541 atatgcctat atctggttat gtaactaata cgagagaatc tatttctaaa ttagcattaa
    48601 aatcaaagaa aatagacatt tcacctaaag gaactttatt aatgagtttt aaattatcta
    48661 ttggaaaagt tgccatattg gatattcctg ccactcataa tgaagccatt atatcgatat
    48721 tcccctatgc taataaagaa aatattatca gagattactt aatgatattt ttgccactta
    48781 tctctacttt aggtgattca aaagatgcta tcaaagggaa aacattaaat agtaccagta
    48841 tctccgaatt attaatccct atttctaatc atgaagagat gaaaagaatc attttcaaag
    48901 ttgatttact ttttcaaaaa gtttctcaac tttttgagta ataagttcct gttcctcaaa
    48961 aggagctaac ggaattaata gctcgctcag tgtagtttta ggaatattat ataaagcttg
    49021 acctgatagt ttagttaatg ctttcaattg tttataaaat aacggagagg acaagttaaa
    49081 taatagaaat tttgaaataa tctctgaact ttcgaatggt gttaattgga aaataaatcc
    49141 accagccaca acaccatcat agtctttatc gattcttgca aactttccaa tatgttctaa
    49201 agaggttgat acaggtgtta ttagctgatt atgttttaaa taaacttgct cagaggagat
    49261 gaattgtgta tcaatgtagt aatcgttatc caacagagaa aattctaaag gcttaatatt
    49321 accaccacgt ataattctaa cacctttatt attaatgctt aaatcgccct tcttgtaaga
    49381 aagacctgta ttcattgaaa aaatatcttt tatttttata acaacccaat tcata
//
LOCUS       DAVKQB010000007        47993 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKQB010000007 DAVKQB010000000
VERSION     DAVKQB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.007"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..137)
                     /locus_tag="VW528_000409"
     CDS             complement(<1..137)
                     /locus_tag="VW528_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9911288.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     G"
     gene            307..378
                     /locus_tag="VW528_000410"
     tRNA            307..378
                     /locus_tag="VW528_000410"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:340..342,aa:Glu,seq:ttc)
     gene            384..457
                     /locus_tag="VW528_000411"
     tRNA            384..457
                     /locus_tag="VW528_000411"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:418..420,aa:Asn,seq:gtt)
     gene            complement(498..1040)
                     /locus_tag="VW528_000412"
     CDS             complement(498..1040)
                     /locus_tag="VW528_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9911289.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(1300..2553)
                     /locus_tag="VW528_000413"
     CDS             complement(1300..2553)
                     /locus_tag="VW528_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9911290.1"
                     /translation="MKCFVGLDISSTKLDVCIMLSDTSTPVTASLSNDLSGATEIKNH
                     ILDLNHTYHFERIVIGMEATSLYSFHPAMFFHEDRQLKELHVEIMVEQPNKIKKYRDA
                     FEENKNDTIDAFYIADYFRIERFSPSFLKEEKYMALQHLTRTRLQIIEQLTRTKQHFI
                     ENIYYKCNTLAYQFKKEELSTNLWSSTMMTLMTDEMTLDELANMPLQELDDWLQKLGK
                     GHFKDSEKLAQTIQAAIRGSYRLSKLQQDSVNVILGLLAREIRNLEKMIKEIDKAIED
                     MVETIPEYQCLTSIPGVGKVYAAGIIAEIGQIERFKDHPQVAKFAGLNWREKQSGNSN
                     SQHTSLVNRGNRYLRYYLVEAANSVRRYDDEYKDFYKKKYHEVPKHQHKRAILLTARK
                     FVRLVDVLLRNHQLYTPPRRIMEDN"
     gene            2909..4667
                     /locus_tag="VW528_000414"
                     /pseudo
     CDS             2909..4667
                     /locus_tag="VW528_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(4697..7240)
                     /locus_tag="VW528_000415"
     CDS             complement(4697..7240)
                     /locus_tag="VW528_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9911291.1"
                     /translation="MKSFLKTYRTYFISLIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLSPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYTLMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVLFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSTYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVDFDY
                     FSPIPYEKIENTNNLISVTSSSNEDAAIQYQIEVPENSQIYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(7302..8924)
                     /locus_tag="VW528_000416"
     CDS             complement(7302..8924)
                     /locus_tag="VW528_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9911292.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            9130..10155
                     /gene="trpS"
                     /locus_tag="VW528_000417"
     CDS             9130..10155
                     /gene="trpS"
                     /locus_tag="VW528_000417"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9911293.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            10307..11785
                     /gene="guaB"
                     /locus_tag="VW528_000418"
     CDS             10307..11785
                     /gene="guaB"
                     /locus_tag="VW528_000418"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9911294.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(11836..12933)
                     /gene="recF"
                     /locus_tag="VW528_000419"
     CDS             complement(11836..12933)
                     /gene="recF"
                     /locus_tag="VW528_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9911295.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(12936..13304)
                     /gene="yaaA"
                     /locus_tag="VW528_000420"
     CDS             complement(12936..13304)
                     /gene="yaaA"
                     /locus_tag="VW528_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9911296.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            13463..14713
                     /locus_tag="VW528_000421"
     CDS             13463..14713
                     /locus_tag="VW528_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9911297.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            14710..15993
                     /locus_tag="VW528_000422"
     CDS             14710..15993
                     /locus_tag="VW528_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9911298.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGYVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTIDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            16035..16856
                     /locus_tag="VW528_000423"
     CDS             16035..16856
                     /locus_tag="VW528_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9911299.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            16867..17412
                     /gene="pgsA"
                     /locus_tag="VW528_000424"
     CDS             16867..17412
                     /gene="pgsA"
                     /locus_tag="VW528_000424"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9911300.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            17409..18236
                     /locus_tag="VW528_000425"
     CDS             17409..18236
                     /locus_tag="VW528_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9911301.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            18221..19060
                     /locus_tag="VW528_000426"
     CDS             18221..19060
                     /locus_tag="VW528_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9911302.1"
                     /translation="MGIALENVNFIYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            19053..19847
                     /locus_tag="VW528_000427"
     CDS             19053..19847
                     /locus_tag="VW528_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9911303.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            19906..20724
                     /gene="mreC"
                     /locus_tag="VW528_000428"
     CDS             19906..20724
                     /gene="mreC"
                     /locus_tag="VW528_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9911304.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            20724..21218
                     /gene="mreD"
                     /locus_tag="VW528_000429"
     CDS             20724..21218
                     /gene="mreD"
                     /locus_tag="VW528_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9911305.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            21312..22490
                     /locus_tag="VW528_000430"
     CDS             21312..22490
                     /locus_tag="VW528_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9911306.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            22714..23493
                     /gene="rpsB"
                     /locus_tag="VW528_000431"
     CDS             22714..23493
                     /gene="rpsB"
                     /locus_tag="VW528_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9911307.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNK"
     gene            23559..24599
                     /locus_tag="VW528_000432"
     CDS             23559..24599
                     /locus_tag="VW528_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9911308.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            24845..25168
                     /gene="tnpB"
                     /locus_tag="VW528_000433"
     CDS             24845..25168
                     /gene="tnpB"
                     /locus_tag="VW528_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9911309.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSDQV
                     FLFCGGRKDRFKVLYWDGQGFWILYKRFENGKLTWSSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(25309..25596)
                     /locus_tag="VW528_000434"
                     /pseudo
     CDS             complement(25309..25596)
                     /locus_tag="VW528_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            25587..25985
                     /locus_tag="VW528_000435"
     CDS             25587..25985
                     /locus_tag="VW528_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9911310.1"
                     /translation="MKAPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEGWARM
                     GLPITRKEIVNWHIKASQYYLEPLYNLLRERLLTQSLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(26182..27102)
                     /gene="cysK"
                     /locus_tag="VW528_000436"
     CDS             complement(26182..27102)
                     /gene="cysK"
                     /locus_tag="VW528_000436"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9911311.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(27387..28022)
                     /locus_tag="VW528_000437"
     CDS             complement(27387..28022)
                     /locus_tag="VW528_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9911312.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            28078..29376
                     /locus_tag="VW528_000438"
     CDS             28078..29376
                     /locus_tag="VW528_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9911313.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLDLYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILEREVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            29373..30035
                     /locus_tag="VW528_000439"
     CDS             29373..30035
                     /locus_tag="VW528_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9911314.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            30115..30663
                     /gene="raiA"
                     /locus_tag="VW528_000440"
     CDS             30115..30663
                     /gene="raiA"
                     /locus_tag="VW528_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9911315.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            30799..32772
                     /locus_tag="VW528_000441"
     CDS             30799..32772
                     /locus_tag="VW528_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9911316.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDTFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDHVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            32769..33221
                     /gene="rplI"
                     /locus_tag="VW528_000442"
     CDS             32769..33221
                     /gene="rplI"
                     /locus_tag="VW528_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9911317.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            33265..34617
                     /gene="dnaB"
                     /locus_tag="VW528_000443"
     CDS             33265..34617
                     /gene="dnaB"
                     /locus_tag="VW528_000443"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9911318.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            34619..34885
                     /locus_tag="VW528_000444"
     CDS             34619..34885
                     /locus_tag="VW528_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911319.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            34974..36320
                     /gene="cbpD"
                     /locus_tag="VW528_000445"
     CDS             34974..36320
                     /gene="cbpD"
                     /locus_tag="VW528_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9911320.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPMIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      36403..36505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            36576..36866
                     /locus_tag="VW528_000446"
     CDS             36576..36866
                     /locus_tag="VW528_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9911321.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVPMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            36829..37587
                     /locus_tag="VW528_000447"
     CDS             36829..37587
                     /locus_tag="VW528_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9911322.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            37625..38632
                     /locus_tag="VW528_000448"
     CDS             37625..38632
                     /locus_tag="VW528_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9911323.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            38632..39360
                     /locus_tag="VW528_000449"
     CDS             38632..39360
                     /locus_tag="VW528_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911324.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            39479..39937
                     /locus_tag="VW528_000450"
     CDS             39479..39937
                     /locus_tag="VW528_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9911325.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            39939..42371
                     /locus_tag="VW528_000451"
     CDS             39939..42371
                     /locus_tag="VW528_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9911326.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            43013..43666
                     /gene="rr06"
                     /locus_tag="VW528_000452"
     CDS             43013..43666
                     /gene="rr06"
                     /locus_tag="VW528_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9911327.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            43663..45003
                     /gene="hk06"
                     /locus_tag="VW528_000453"
     CDS             43663..45003
                     /gene="hk06"
                     /locus_tag="VW528_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9911328.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            45098..45625
                     /locus_tag="VW528_000454"
     CDS             45098..45625
                     /locus_tag="VW528_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9911329.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            45704..>47993
                     /locus_tag="VW528_000455"
     CDS             45704..>47993
                     /locus_tag="VW528_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEU9911330.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLVMGSVVHATENEGIT
                     QVATSYNKANESQTEHRKAAKQVDEDIKKMLSEIQEYIKKMLSEIQLDKRKDTQNRTL
                     NRKLSAIQTKYLYELRVLKEKSKKEELTSKTKKELDAAFEKFKKEPELTKKLAEAKQK
                     AKAQKEEDFRNYPTNTYKTLELEIAEFDVKVKEAELELVKEEAKPRNEEKIKQAKEKV
                     ESKKAEATRLEEIKTERKKAEEEAKRKAEESEKKAAEAKQKVDTKEQGKPKRRAKRGV
                     SGELATPDKKENDAKSSDSSVGEETLPSPSLNMANESQTEHRKDVDEYIKKMLSEIQL
                     DRRKHTQNVNLNIKLSAIKTKYLYELSVLKENSKKEELTSKTKAELTAAFEQFKKDTL
                     KPEKKVAEAEKKVEEAKKKAKDQKEEDRRNYPTNTYKTLELEIAESDVKVKEAELELV
                     KEEANESRNEEKIKQAKEKVESKKAEATRLEKIKTDRKKAEEEAKRKAEESEKKAAEA
                     KQKVDAEEYALEAKIAELEYEVQRLEKELKEIDESDSEDYLKEGLRAPLQSKLDTKKA
                     KLSKLEELSDKIDELDAEIAKLEVQLKDAEGNNNVEAYFKEGLEKTTAEKKAELEKAE
                     ADLKKAVDEPETPAPAPQPAPAPEKPAEKPAPAPEKPVEKPAPAPEKPVEKPAPAPAP
                     EKPAPAPEKPAPTPETPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMA
                     TGWLQNNGSWYYLNSNGAMATGW"
BASE COUNT        15236 a         7878 c        10276 g        14603 t
ORIGIN      
        1 tttcctttct gttgataggc tagtaacaga cgattgattt gtctttcaga aagattgagt
       61 tcgacacagg cccgtttctt tgttttcttt ccttgggcta tagcttttat cacaagatat
      121 tttttcgttt cattcatatt cagttggatc cttttcatat gactattcta ccaaatggga
      181 cattttcacg ttcgatttac taaagacatt atcacattcg aattacatat tttagagaaa
      241 aaatctcaaa agtgattgac aattagcaag aaattgatat aatggttata tctgctacag
      301 atagaaggtc cgttggtcaa ggggttaaga caccgccttt tcacggcggt aacacgggtt
      361 cgaatcccgt acggactatt ttatccgcca tagctcagtt ggtagtagcg catgactgtt
      421 aatcatgatg tcgtaggttc gagtcctact ggcggagtat agataaaagg gaacacagct
      481 gtgttcctct ttttgtatca atttgtatca ccaagcattt tcataaggaa gtctgttatt
      541 tcttgagaac tttctttttt tccatgtgca atccaagttt ggcagacacc aaaaagtgca
      601 tgagttagat agatgctact atattctaat tcagtggtat ttagattcag ttgcataaat
      661 cgcttttgta aatctgtact aagcatgata tgaagtttat ttcgtaagaa attttggatt
      721 tctttagtcc cattttcaga aagaagggca gccagaagtg gttctgactc tagatattca
      781 aaaacttcta aaatagcgtc tcttttgtga tgagcatgtt tttgaaaaat atattcaaat
      841 gtatggaata gcttgctttg atagtgctca atcatatcat acttatcctt atagtgagta
      901 tagaagctgg aacgactaat tccggctttt tctactaatt tgacagtaga aattttatca
      961 aatggctgtt ccatcagtaa ttgtaccata gcgttttcaa tagttcgctt tgtttttaag
     1021 cgtttgttac tttcttgcat atttcctcct tgtaaacaaa ttagactata tgtctaaaaa
     1081 tagatttttt atcttgtaat ttagattttt taatgtataa tctattatat caaaatttta
     1141 gacaatatgt ttaaaaaagt ctagtggtaa aatgtcaagc gaccccccgc tcaaagtttg
     1201 aaaactattt caaaaaaagg gtgcactaaa gaagccctac tgaaaacgat gttttcacac
     1261 tgtttttagt agattttcaa ggagaggtgc agatataact tagttatctt ccataatcct
     1321 ccttggtggc gtatagagtt gatggttgcg tagtagcaca tccaccagac gcacaaattt
     1381 tctagcggtt agtaagatgg ctcgtttgtg ttggtgttta ggtacttcat ggtatttctt
     1441 cttatagaaa tccttgtatt cgtcatcata acgccttacc gagttggcgg cttcaaccag
     1501 gtagtagcgc agataacgat tgcccctatt cacaagcgaa gtatgttgtg agtttgagtt
     1561 accagattgc ttttctctcc aattcagacc agcaaatttt gcgacctggg gatggtcttt
     1621 aaagcgttcg atttgtccaa tttcggcaat gataccagca gcgtagacct tcccgacccc
     1681 aggtatggag gtcaggcatt gatattcagg aatggtttcc accatatctt caatggcctt
     1741 atcaatttcc ttgatcattt tttccaggtt tcgaatttcc cgagccagaa gtccgagaat
     1801 gacattgact gaatcttgtt ggagtttaga gaggcgataa gaacctctga tagcggcttg
     1861 aatggtttga gcaagcttct cagagtcctt aaaatgccct ttacccagtt tttggagcca
     1921 atcatctaat tcttgaagag gcatgttagc aagttcgtct aaggtcatct catctgtcat
     1981 caaggtcatc atcgttgaac tccataggtt agtagagagt tcttctttct tgaattgata
     2041 agccaaggta ttgcacttgt agtagatatt ttcaatgaag tgctgtttgg ttctggttaa
     2101 ttgctcgatg atttggagtc tggttcttgt gagatgttgg agagccatgt atttctcttc
     2161 ttttaaaaaa gaaggtgaaa atcgctcaat acggaaataa tccgcaatgt agaaagcatc
     2221 aatggtatcg tttttgtttt cttcgaaagc atcccgatac ttcttgattt tattaggttg
     2281 ttcgaccata atctcaacat gtagttcttt caactgccta tcttcatgga agaacatagc
     2341 agggtggaaa ctgtagagac tggttgcttc catgccaatg acaatccttt cgaagtgata
     2401 ggtatgattc aaatcaagga tatgattctt aatctctgtt gccccagaaa ggtcattaga
     2461 gagggaagca gtgacggggg tgctagtatc actcaacatg atacacacat ctaattttgt
     2521 agagctgatg tctaaaccga caaaacattt cattaaggaa ggtctccttt ccattagatt
     2581 tggaagtttt tttgactact gtaaggtttc ccagaaacac cttgttccaa catcctcgca
     2641 taagagaatc cttatcacag cctgacagag ccgcctgcat cctactagat acagaagtca
     2701 agctgtggaa acagctagtg tgttggaagt ttgaacaagg cttgggtaac agacttttta
     2761 atgaagtcca agcaacaagg agaaaaagaa acaaccatga gtccattcca tgactctatt
     2821 gttctagaaa accttgcaat agtcaagtga taacgaataa aacttgggat tgaaatatcc
     2881 ccatgattat atattacgag gagaaactat gtttaaagaa tggaaagcaa tttaaaaaaa
     2941 acaaccttta ttattgtcat gatcgggatt tctcttattc cagctctgta caatatcata
     3001 tttttgtcat caatgtggga tccatatggg caattgtctg acttacctgt ggcagttgta
     3061 aataatgata aagaggcttc ctataatggt aatactatgg caataggaaa agacatggtg
     3121 tccaatttaa aagaaaataa aaccttggat tttcattttg tagatgaaga ggaaggaaag
     3181 aagggattgg aagatggcga ttactatatg gtagtgactt taccaagtga tttatctgaa
     3241 aaagcaacta cattatccaa tattcaatcg acagcagctt atcaatcatt gacaagtgag
     3301 caacaaactg agataagtga ttctgtatct caaaattcaa ctgatagtat tcaatcggct
     3361 cagtcaattg tagctttagt acaagattta cagggaagtt tagaaaactt acaaaatcaa
     3421 tcttctaatc tttcgacttt aaaaaatcaa gctaatcaag tattacctat tacttctact
     3481 tctttgatag gattgtcaag tggattaaca gagatacaag gagctgttac tagcaaatta
     3541 gttcctgcca gtcagtcgat tgcatcaggt gtaaacgcat atactacagg tgttgataaa
     3601 gtttctcagg gcgcaagtca actaagtgaa aaaaatgcta ccttgacagg tagtttggat
     3661 caattagttt caggctcaaa caccttgaca caaaaatctt ctagcttgac agcaggagtt
     3721 ggtcaattag ttgaaaaaac tccagaatta gtatctggta ttgaaaaatt atcaactggc
     3781 tccaattaat tgaatcaaaa gagtcaagaa ttgatagcag gagttgataa attgcagtca
     3841 ggctctagcc aactagctga caaatccagt cagttacttt caggtgtttc tcaattagaa
     3901 agtggagcta ataaattggc agatggagct gggaaactag cagaaggtgg aacaaagtta
     3961 acttctggat tggaaggttt acagacagga cttgcttctt taggacaagg actaggtaat
     4021 gctagtgatc aactcaaatc agcatcaaca gaatctaaaa atgcagagat tttgtcaaat
     4081 ccactcaatc tttccaaaac agacaatgac caagttcctg taaatggaat tgcaatggct
     4141 ccttatatga tatcagttgc tctttttgtt gcagcaatat caacgaatat gatctttgcg
     4201 aagttacctt caggacgtca tccagagagc cgttgggctt ggttgaaatc tcgagctgaa
     4261 ataaatggta ttatagctgt tttggcaggg attttggtat atggaggagt tcatcttatt
     4321 ggtctaactg ctaatcatga aatgagaaca tttattctca tcatcttaac aagtttagta
     4381 ttcatgtcta tggtgaccgc tttaacaaca tggaatagcc gtataggagc tttcttctcg
     4441 cttatttttc ttttattaca gttagcatca agtgcaggta cctatccgct tgctttgaca
     4501 aatgatttct ttagagctat taatccttgg ttgccaatga gttattcagt ttcgggatta
     4561 cgacaaacaa tctccatcaa caagtcattt tcctagctgt catactagct ctatttactg
     4621 gtttaggtat gctagcctat caacctaaga aaatggaaga agattaaaaa aatcgaccga
     4681 ttaactggtc gattttttat gccttagatg actttcgtct gtgattatag attccaaata
     4741 gtaagagaga agtaaaggaa cagattgctc cagtaataaa accattggga atgaaggaaa
     4801 gtgtaatagt tcctttcccc ttgggaatgt caactttcat aaatccagtt tgagcttgtt
     4861 taatttctat tttcttacca tcttggtagg cagaccaacc tttgtcataa ggaatggtga
     4921 agaaaataga tgtatcttgt tggacatcat atgtagcaaa aaccttgttt ttagaagttg
     4981 atactgtgac aggttgttct ttaatttttt gaattgcctc ggtgaaagtt ttggtatcta
     5041 aacgatagaa ggtaggagat tcaaatgata cttgtgaatt tccagggaaa ctaacattga
     5101 tattgaaagt ttttttctct ttagtatatc ctagattaaa gaaggagaag acattatcag
     5161 ttgtaaaagt ctttttttca ccatttacaa ggatgtcaac cttcttttgt ttatcgttag
     5221 aaaagtgaag gtttatgaaa gagagataaa tttggctgtt ttctggaact tcaatttgat
     5281 actggattgc tgcatcctca tttgaagaac ttgtgacact aatcaaatta ttagtatttt
     5341 ctattttttc ataagggatt ggagaaaaat aatcaaaatc aacgttagca agttgattta
     5401 aaaacgaggc ctgattatcc aaggtatgtt cattgaactt gacatcattg taaacagatt
     5461 gactagcaac tgcaatcgga agagagtatt gattttcata tagggtaaga ttatcttttt
     5521 gatagatatc tttaaaacca tacttatcaa taggactgtc tgagatattg tactggatac
     5581 caaataaact atcagccaaa atactattat ttgcatatcg gagattgaga ttagtcccag
     5641 aggatttaaa cccaagttta tctaaagtag agcttgatga acgatttcga acagatgaaa
     5701 attgagagat tccattgtag ttgaatttca tactgtcatt tcctgtctga gtttgtagtt
     5761 tttcagtacg agtaaattga tttccaatat atgttgagaa agattccata gctgggatat
     5821 ctcgactata agtacttcga gaagcaaatc cccattcctt agcaattccg tccatttgag
     5881 atgaagcatt taaactcatt tcaaccagta taaataaaga gattagaatg gcaaatagat
     5941 tcacagatat aaactttttg ataactgcaa ggagtaaaag agaatagaca accaaaaatt
     6001 caagagtaag cagaatattc aaatctgtta aaaaagaata atgcgatttt agatagatgg
     6061 tagctaaaaa tcctgttact acaagaaaaa gcgaaactaa aaaattccag actttaagtt
     6121 ctttcagacg ctttaagact tctgctgctg tgtaaattaa caaggtagag aaaatccaag
     6181 catagcgatg taaaaacatg tttggagtat gcatgccttg ccaaaataag tcaagagctt
     6241 ctatgtaaaa gcttgcaatt agaaatgcaa agaataatac atatatgagt ttcacgtgaa
     6301 acttaataga tttcagcgta aaaaataaaa tggtcaaaat aaaaggaagt agtccaacaa
     6361 aaatcattgg gatggcccca tactttgttg tgtcaaagga accaatgaat tgcttagcaa
     6421 agagatcaag ataccagcta ctttcagttt gaaactttgt aacttcagtc aatttttccc
     6481 catgtgtctg taaatcaaac agagtgggaa gagtcataat caaactagcc ataccagcta
     6541 aaaaggagat aactatgaaa tcaagaacag atgattttcg agtcttaaag tcccacgaaa
     6601 tttgacagag ataccagaaa ataagaaaca atactgtcat atatccaaaa taataatttt
     6661 gaataaataa gattgacaga cttgtaaagt acaataggag tttcttttca gttatcagta
     6721 gatgtaaacc agttataatt aaaggaatca agataaaaac atctagccag gtttttatct
     6781 ctaattgact gacagtgaaa ctcatcagag tataggaagt agataaggct agttttaaag
     6841 tctgagggat agattgaaac aatttattca aactaaaaaa ggttgacaga ccaatcaatc
     6901 caaattttaa gagagtggtc agataaacag catctggcat attcgttaga tcaaaaaagt
     6961 aaaccagagg tgagagaaaa ctacccaagt aataactaga cagagcatag aagtttagcc
     7021 ctagaccact tgtaaaggtg taaaacagac tactatttcc atgtaggata tttcgtaagg
     7081 ctacatcaaa aataacgtat tgatgaaagc catctcctaa tagaggagag ttgtcgctat
     7141 tccagtagat accttgagat agatatactc cagacataat cactacagga atgatgagag
     7201 aaataaaata ggttcgatat gtttttaaaa atgatttcat gttacctcgt agaatgatag
     7261 aaaactcagt tggttaaccc aactgagttt tgaagtttta tttagtcttt ccaaagttct
     7321 ttaacttttg cttgtacttc tgcattttct aggaattcat cgtaggtttc atcgatacgg
     7381 tcaatgacgc catttttaga taagacaatg atatggttag ccaaagtttg aataaattcg
     7441 tggtcatggc tagcaaagat gattgattct ttaaagtttt tcaatccatc attcaagctt
     7501 gagatagatt ccaagtccaa gtgatttgtt ggatcatcaa gtacaaggac atttgatttt
     7561 aagagcatga gttttgaaag catgacacga actttttctc cccctgacaa gacatttaca
     7621 ggtttgttaa cttcatctcc agagaagagc atacggccga ggaagccacg taggaaagta
     7681 ttgtcatctt cttctttact tgcgaattga cgcaaccagt caagaattga ttctcctcct
     7741 gcaaaatcag ctgagttatc ttttggtagg taagattgac tagttgtaac tccccacttg
     7801 acagttcctt catagtcaat atctcccatg attgcacgaa ttaatgcagt cgtttgaata
     7861 tcattttgtc caataagtgc tgtcttatca tctggacgca agatgaaact aatattatcc
     7921 aagatagttt caccatcaat ctttacagtt agattttcta ctgtcaagag atcattacca
     7981 atctcacgtt ccgctttaaa gttgataaat ggatatttac gactagatgg tacaatctct
     8041 tctagctcaa tcttatcaag cattttctta cgtgatgttg cctgccttga cttagaagca
     8101 ttggcagaga aacgagcaac gaattcttgc aattgtttaa ttttttcttc tgctttagca
     8161 ttacggtctg ctagcaattt agcagcaagc tcagaagatt ccttccagaa gtcgtagttt
     8221 ccgacataga gtttgatttt tccaaagtca aggtcggcca tgtgagtaca aactttgttt
     8281 aagaagtgac ggtcgtggga tactacgata actgtgttat caaagtcaat caagaagtct
     8341 tctaaccaag taatcgattg gatatccaaa ccgttagtag gctcgtccaa gagaagaaca
     8401 tctggtttac caaaaagtgc tttggcgagg agaaccttta ctttttcacc gttggccaat
     8461 tcgctcatgt tttggtagtg taattcttct ggaatgttta ggttttgaag tagttgagag
     8521 gcttcactct ctgcttccca acctccaagt tcggcaaact ctccttcgag ttcggcagca
     8581 cgaaccccgt cctcgtctga gaaatcttcc ttcatgtaga tagcatcttt ctctttcatg
     8641 atgctataaa gtttttcatt tcccatgata acgacatcaa tggcacgttc atcttcgtag
     8701 tcaaagtgat tttgacgaag aacagagaga cgttcatctg gaccaagaga gatgtgacca
     8761 gtagtaggtt cgatatctcc agctaaaatt tttaaaaagg ttgattttcc ggcaccatta
     8821 gcaccgatta atccgtaagt atttccttct gtaaatttga tattgacatc atcaaaaagt
     8881 ttgcgatcac taaaacgtag tgaaacatca gatactgtaa gcaatatttt tctcctatat
     8941 gtgtaatata tttattctac tagaaaatac agaaatattc aaatttttat ttgtcaattt
     9001 tgtgtaaatt atatttacag tatcctttac acaaatctgt aaaaagcaag gctgatttat
     9061 tttgataaat tacggttatt tcattaaaaa aatgctataa ttgaaaggac tatatcgaag
     9121 gagaacaaaa tgactaaacc cattatttta acaggagacc gtccaacagg aaaattgcat
     9181 attggacatt atgttggaag tctcaaaaat cgagtattat tacaggaaga ggataagtat
     9241 gatatgtttg tgttcttggc tgaccaacaa gccttgacag atcatgccaa agatcctcaa
     9301 accattgtag agtctatcgg aaatgtggct ttggattatc ttgcagttgg attggatcca
     9361 aataagtcaa ctatttttat tcaaagccag attccagagt tggctgagtt gtctatgtat
     9421 tatatgaatc tagtttcgtt agcacgtttg gagcgaaatc caacagtcaa gacagagatt
     9481 tctcagaaag gatttggaga aagcattccg acaggattct tggtctatcc aatcgctcaa
     9541 gcagctgata tcacagcttt caaggctaat tatgttcctg ttgggacaga tcagaaacca
     9601 atgattgagc aaactcgtga aattgttcgt tcttttaaca atgcatataa ctgtgatgtc
     9661 ttggtagagc cggaaggtat ttatccagaa aatgagagag cagggcgttt gcctggttta
     9721 gatggaaatg ctaaaatgtc taaatcacta aataatggta tttatttagc tgatgatgcg
     9781 gatactttgc gtaaaaaagt aatgagtatg tatacagatc cagatcatat ccgcgttgag
     9841 gatccaggta agattgaggg aaatatggtt ttccattatc tagatgtttt tggtcgtcca
     9901 gaagatgctc aagaaattgc tgatatgaaa gaacgttatc aacgaggtgg tcttggtgat
     9961 gtgaagacca agcgttatct acttgaaata ttagaacgtg aactgggtcc tattcgtgag
    10021 cgccgtattg aatttgctaa ggatatggga gaagtttata atatgattca aaaaggtagt
    10081 gaaagagcgc gtgaagttgc gggtcaaacc ctatctgagg taaaaggggc aatgggactt
    10141 cattacttta actaagttta ttttacaaga aactatcatt tctatctcga agaaaagata
    10201 gttttttatt tacccctgta acatttgaca aatttttata gaatggtatg atagatacaa
    10261 tataaaaaga gtcaagctca aaaagaaaga aaagaggaaa cttcgaatgt ctaattggga
    10321 cactaaattt ttgaaaaaag gttttacctt tgatgatgta ttgcttattc cagctgaaag
    10381 tcatgtgttg cctaacgatg cagatttaac aactaaattg gcagataatt tgactttaaa
    10441 tatcccaatt attaccgctg ccatggacac agttacagag agtcaaatgg ccattgctat
    10501 tgctcgtgca ggtggtctcg gagttatcca taaaaacatg tcaattgctc aacaagcaga
    10561 cgaggttcgt aaggtaaaac gttctgaaaa tggagttatt attgatccgt tcttcttgac
    10621 gcctgaacat acaattgctg aagcagatga gcttatgggt cgttaccgca tcagtggtgt
    10681 tccagttgtt gaaacacttg aaaatcgtaa attggttggt attttgacaa accgagatct
    10741 tcgttttatt tcagattata atcaaccaat ttcaaaccat atgactagtg aaaatcttgt
    10801 tactgctcct gtgggtacgg atcttgcaac ggctgagagt attcttcaag agcatcgtat
    10861 tgaaaaactt ccgttggtcg atgaagaagg cagtctttct ggtttgatca ctatcaaaga
    10921 tattgaaaaa gttattgagt ttccaaatgc ggctaaagat gaatttggtc gtcttctagt
    10981 tgcaggtgca gtaggtgtta cttcagatac atttgaacgt gcagaggctc tttttgaggc
    11041 aggagcggat gcgattgtta ttgatactgc acatggtcat tctgcaggtg tcttgcgtaa
    11101 aattgccgag attcgtgctc atttcccaga tcggactttg attgctggaa atattgctac
    11161 tgctgaaggt gcacgtgccc tttatgaagc gggtgtagac gttgttaagg ttggtattgg
    11221 accaggttct atctgtacta ctcgtgtgat tgctggtgtt ggtgttccgc aagtaacagc
    11281 tatctacgat gctgcagctg ttgcgcgcga atatggtaaa acgattattg ctgacggtgg
    11341 gatcaagtat tctggagata ttgtaaaagc acttgctgca ggtggaaatg ctgttatgct
    11401 tggatctatg tttgctggaa ctgatgaagc tccaggcgaa actgaaatct tccaaggacg
    11461 taaattcaag acttaccgtg gtatgggatc aattgctgct atgaagaaag gttcaagcga
    11521 ccgttatttc caaggttctg tcaatgaagc aaacaagctc gttccagaag gaattgaagg
    11581 tcgtgttgct tataaaggag cggcagctga tattgttttc caaatgattg gtggtattcg
    11641 ctctggtatg ggttactgtg gtgcagctaa ccttaaagaa ctacacgata atgctcaatt
    11701 tattgaaatg tctggtgctg gtttgaaaga aagccatcct catgatgtgc aaattactaa
    11761 tgaggcacca aattattcta tgtaaaaaac aatgaaaaga actccagtga aaacaggagt
    11821 tcttttacaa tgttgtcaat ttccatttac agaagcttta ccatcctgaa tagtgaagat
    11881 acttagattt tctggcagat tttgaagatg gtctaagctt gttgttgtga taaaggtttg
    11941 gattgattga gaaatcgttt ctaataattt taactgtcta gtgttgtcaa gttcactcat
    12001 cacatcgtca agcagtaata taggagattc tgtggtaatg ctttccatta attcgatttc
    12061 tgctaatttt atcgagagga cgagactacg atgttgacct tggcttccga aactagcatc
    12121 catcccattt atataaaaag aaatgtcatc tcgatgagga ccgacaccag tattcttttt
    12181 aaataaatct ctggatctac ttttttctaa agcaattttg aaagattcgg ataagttttg
    12241 tttgtcagtt atattgacag aagattgata ggatattgac aactcttcga tctgattaga
    12301 gagttcaaaa tgtttcttac gcccaaatga ttctagtttt tttatgaaat ctaagcggtg
    12361 attcattaca cgacatccat aatcaactag ctgatcatct aacacagaaa ggaatgtttc
    12421 atctattttt tgagctgatt ttaggtaagt gtttctttgc tttaggatgt ggttataatt
    12481 ggttaagtca gataaataga ttggcttaat ttgcccaagt tccatatcaa tgaattttcg
    12541 tcgaatcgaa ggtgctcctt taattagttg taaatcttca ggagcaaata agacaacatt
    12601 catgtgtcct acataatctg aaaggcgtgc ctgttttaag tgattaactt ttgttacacg
    12661 gcctttttgt gttagttcga tttctagggg aatggatcca gtttttttct gaacgagacc
    12721 tgaaagatga agttgttcct catcaaaatg aatgagattt ttatctgttc gagttcgatg
    12781 actacgcgtt aaggctaaaa aatagatagc ctctaacatg tttgtttttc cttgtgcatt
    12841 acgtcccaaa aagacattca atttaggatt aaagtctatt ttcgtttctt tgtaattacg
    12901 aaaagtcttg agagatagat gttgtagcca catgattatc taccagggaa tcgtggagct
    12961 tgttggcttt taggtgatga agcaggtttc gatttgtctt ttttaactcc cttattcatc
    13021 tttttgacaa gtttagcgat ccgttctttt tcaactttat cagcttggta ttcatcttgt
    13081 tcttcagaag taggttgtgt caacaagatg tcaatgttca tgtcagggat gtcaacttta
    13141 tcgccaatac gaagtttttt tccacgacga ctctctaatt ccctattaaa gtaaacagaa
    13201 tgttcagaga gaaatgattt aatagctcct ccgctatgtg taattccaag ttctttgagt
    13261 agtgcttgga gggtaataaa ttcttcaaat aatttgtatt ccataattca cctcaatctt
    13321 tggtattata ccatattttc ctaaaaatag tgagagagac agcgagaaat gtaagcgatt
    13381 tttatttcaa aagtgttaca gggaacaaga aaatttcagg ttttcgtgat ataatagaag
    13441 tctgtatata aggaggtaaa tcatggagtt agtgcatgga atttcaacac attttatcca
    13501 atcaaaaaag tttaaaacaa acaaaattac cgtgcgtttt accgctccat tatcccttga
    13561 tacgattgca ggtcacatgt tgagtgcaag tatgctagag actgctaatc agatgtaccc
    13621 cacttctcaa gatttgagga gacacttggc cagtctatac ggtacagata tgtcaaccaa
    13681 ttgtttcaga agagggcaaa gccacattat agaattgaca tttacctatg ttcgtgatga
    13741 gtttttaagt aggaaaaacg tgctaacctc tcagattttg gaacttgtaa aagaaactct
    13801 tttttcaccc gcagtagttg ataatgggtt tgatccggcc ttatttgaaa ttgagaaaaa
    13861 acaattgcta gcaagtttag cagctgatat ggatgattct ttttattttg cacataaaga
    13921 attggataaa ttgttttttc atgatgaacg tcttcaattg gaatatagtg atttacgaaa
    13981 tcgtatttta gctgaaactc cacaaagttc ttattcttgt ttccaagaat ttttagccaa
    14041 tgatcgaata gatttctttt tcctaggtga ttttaatgag gttgaaattc aaaatgtatt
    14101 agaatcattt ggctttaaag gtcgaaaagg agatgtgaag gttcagtatt gtcaacctta
    14161 ttctaatatc cttcaggaag gtatggttcg gaaaaatgtg ggacaatcca ttttggaatt
    14221 aggttatcat taccgttcta aatatggtga tgagcaacat ttacccatga ttgtaatgaa
    14281 tggtttactt ggtggatttg ctcactctaa gctctttaca aatgtccgtg aaaatgctgg
    14341 attagcttat accatttcaa gtgagcttga tttatttagt ggattcttga ggatgtatgc
    14401 tggtatcaat cgagaaaatc gtaaccaggc tcgtaaaatg atgaataatc aactgcttga
    14461 tttaaaaaaa ggttatttta cagagtttga gttaaatcag accaaggaaa tgattcgttg
    14521 gtcgttgtta ctttctcaag ataatcaatc ttcattgatt gaacgtgctt atcaaaatgc
    14581 cttatttgga aaatcttcag cagactttaa aagttggatt gcaaagcttg aacaaattga
    14641 caaagatgct atttgtagag tagctaataa tgtgaaacta caagcgattt actttatgga
    14701 aggaatagaa tgacaaaggt tgtttttgaa gaaaaatact atccagctgt aaaagaaaag
    14761 gtttatcgaa ctcgtttggc caacggattg acagttgctc ttttgcctaa aaaggaattt
    14821 aaagaggttt acgggagtgt cactgtacag tttggttcgg tagatacgtt tgtcacagaa
    14881 gttgacggat atgtaaaaca atatcctgga ggaattgctc attttcttga acataaatta
    14941 tttgagagag aagattctag tgatttgatg tcggctttta cgagtctagg tgcagatagt
    15001 aatgccttta caagctttac aaaaacaaac tatctttttt cagcaacgga ttatttttta
    15061 gaaaatttag atttacttga tgaattggta acatcagcac actttactga agcttccatt
    15121 ctgacagagc aggatattat tcagcaagaa cgagaaatgt accaagatga tccagattcg
    15181 tgtttattct tttcaacttt agcgaatttg tatcctggta cacctttagc aactgatata
    15241 gttggaagtg aggagtccat ttcccaaatc aatctaacta atttgcaaga aaattttaca
    15301 aagttttaca aacctgtaaa catgtctctg tttttagttg gtaattttga tgtggagcga
    15361 gtacaggact attttgaaag caaagaactg aaagattcag attttcagga agtagcaaga
    15421 gaaaagttgt ttttacagcc tgtaaagcca acagatagta tgagaatgga agtatcttct
    15481 cccaaactag cgattggagt tagaggtaag cgagaagttt ctgaagcgga ttgctatcga
    15541 catcatattt tattaaaatt attgtttgca atgatgtttg gttggacttc ggatcgtttt
    15601 caaaaatgtt atgaatcagg taaaattgat gcgtccttat ctctggaagt tgaaataaca
    15661 agtcgctttc attttgtcat gttgacaata gatacgaaag agccagttgc tttgtctcat
    15721 caatttagga aggctattcg taattttaca aaggatttag atattacaga ggaacattta
    15781 gatattatca aaagagagat gtttggcgaa tttttcagta gcatgaactc tcttgaattt
    15841 attgcaacgc aatatgatgc ttttgaaaat ggtgagacaa tttttgattt gccgaaaatt
    15901 ttacaggaaa ttactttaga ggatgtcctt gatgctggac atcatttaat agatgatggt
    15961 gacatagttg attttacaat attcccatcg tagtaaccta tcataataga cactagaaag
    16021 aagggatgac aagtatgaga aaaaaaacaa ttggagaggt tttacgatta gctagaatca
    16081 atcagggatt gagtttagat gaattgcaga aaaagacaga aatccagtta gatatgttgg
    16141 aagcaatgga agcagacgat ttcgatcaac ttccaagtcc tttttacacg cgttctttct
    16201 tgaaaaaata tgcatgggct gttgagttag atgaccaaat tgttttggat gcttatgatt
    16261 ctgggagtat gattacttat gaggaagtag atgttgatga agatgagttg acaggtcgta
    16321 gacgttcaag taagaaaaag aagaaaaaaa catcattttt acctttattt tattttatcc
    16381 tttttgcttt atcgatttta atttttgtga cttattatgt ttggaactat attcaaactc
    16441 aaccagagga gccttctctt tctaattaca gtgtggttca atcaacaagt tcaactagct
    16501 ctgttcccca ctcctcaagt agtagttctt ctagtataga atcagctata agtgtatcag
    16561 gcgaaggaaa tcatgtagaa atcgcttata agacaagtaa ggaaacagtt aaattgcaat
    16621 tggcagtttc agatgttaca agttgggtca gtgtttcaga aagcgaactt gagggcggtg
    16681 taaccttatc gccaaagaag aaaagtgcag aagcaacagt tgcaactaaa agtcctgtaa
    16741 caattacgtt aggtgttgta aaaggtgttg atttgacagt agataatcag actgttgatt
    16801 tatcgaaatt aacagctcag actggacaaa tcactgtaac ctttactaaa aattaaggaa
    16861 aaacgaatga aaaaagaaca aattcccaat ctcttaacaa taggtcgaat tctctttata
    16921 cctattttta tctttatttt gacgatagga aattcgatag agagtcatat agttgcagct
    16981 attatctttg ctgttgccag tattaccgac tatttagatg gatatttagc tcgtaaatgg
    17041 aatgtggtca gtaattttgg taaatttgca gatcctatgg cggataagtt actagttatg
    17101 tcggctttta ttatgttgat tgagttaggt atggctccgg cttggattgt tgcagtgatt
    17161 atctgtcgtg agttagctgt gacaggttta aggcttttat tggttgaaac tggtggaaca
    17221 attttagcag cagcaatgcc tggaaaaatt aaaactttta gtcagatgtt tgctattatt
    17281 ttcttgctat tacattggac tttgcttggt caagttctac tttatgtagc cttatttttc
    17341 actatctact ctggctatga ctatttcaag ggtagtgcct atgtatttaa agggacattt
    17401 ggttcgaaat gaaatcaata attgatgtaa aaaatctttc ttttcgctat aaagaaaatc
    17461 agaactacta cgatgtgaag gatattacgt ttcacgtgaa acgtggagaa tggctttcga
    17521 ttgtagggca taatggtagt ggtaaatcaa cgacggttcg attaattgat ggcttactgg
    17581 aagcagaatc cggagagatt gtaattgatg gccaacgttt gactgaggaa aatgtttgga
    17641 atatacgtcg tcaaatcggt atggtttttc aaaatccaga caatcaattt gttggagcga
    17701 ctgttgaaga tgatgttgct tttggtttgg aaaatcaggg actttctcgt caagaaatga
    17761 aaaagagagt ggaagaagct ctggctttag ttggcatgtt ggactttaaa aagagagagc
    17821 cagcgcgtct atcaggtggc caaaagcaac gtgtggccat tgcaggtgtt gtagccctaa
    17881 gaccagctat tttaatctta gatgaagcaa cgagtatgtt ggatcctgag gggcgtagag
    17941 aacttattgg gacagtaaaa ggaattcgaa aagactatga tatgacggtc atttctatta
    18001 cccatgattt ggaagaagtc gccatgagtg atcgcgtatt ggtcatgaaa aaaggggaaa
    18061 ttgaatcaac tagtagtcca agggagcttt tctctcgaaa tgatttagat caaattggat
    18121 tagacgatcc ttttgctaat caattaaaaa aatctttgag ccagaatggc tatgatttac
    18181 ctgaaaatta tttgacagaa agtgagctag aggataagct atgggaattg ctctagaaaa
    18241 tgtgaatttt atatatcaag aaggtactcc cttagcttca gcagctttgt cggatgtttc
    18301 tttgacgatt gaagatggct cttatacagc tttaattggg cacacaggta gtggtaaatc
    18361 aactatttta caactcttaa atggtttatt ggtgccaagt caagggagtg tgagggtttt
    18421 tgatacctta atcacctcga cttctaaaaa taaagatatt cgtcaaatta gaaaacaggt
    18481 tggcttggta tttcagtttg ctgaaaatca gatttttgaa gaaacggttt tgaaggacgt
    18541 tgcttttgga ccgcaaaatt ttggagtttc tgaagaagat gctgtgaaga ctgcgcgtga
    18601 gaaactggct ctggttggaa ttgatgaatc actttttgat cgtagtccgt ttgagctgtc
    18661 agggggacaa atgagacgtg ttgccattgc aggcatactt gccatggagc catctatatt
    18721 agtcttagat gagccaacag ctggtctaga tcctctaggg agaaaagagt tgatgaccct
    18781 gttcaaaaaa ctccaccagt cagggatgac catcgtcttg gtaacgcatt tgatggatga
    18841 tgttgctgaa tatgctaatc aagtctatgt aatggaaaag ggacgtttag taaagggggg
    18901 caaaccaagt gatgtctttc aagacgttgt ttttatggaa gaagttcagt tgggagtacc
    18961 taaaattacg gccttttgta aacgattggc tgatagaggc gtgtcattta aacgattacc
    19021 gattaagata gaggagttca aggagtcgct aaatggatag tatgattttg gggcgttata
    19081 tcccagggga ttcgattgtt caccgattgg atccacgtag caaattgctt gctatgatgc
    19141 tactgatttt gattgtgttt tgggctaata accccttgac gaatctgatt ctttttatag
    19201 cgacagggat atttattgcc ttgtcaggag tatctctttc attttttatt cagggcttga
    19261 agtctatgtt tttcttgatt gccttcacaa ctatttttca actatttttc atttctaatg
    19321 ggaatgtttt atttgagttt tcgtttgtga gaatcacgga ttatgctttg caacaagctg
    19381 ggattatttt ttgtcgcttt gtattgatta ttttcttttc aactttgtta accttaacaa
    19441 ccatgccttt aagtttggca tcagctgtcg aagctttatt agcaccttta aagcgtgtga
    19501 aagttccagt tcatgaaatt ggattgatgc tgtctatgag tttgcgtttt gtcccaacct
    19561 tgatggatga tacgactcgg attatgaatg cacagaaagc tcgtggagtg gattttggag
    19621 aaggaagcat cgttcaaaaa gtaaaggcga tgattcccat tttgattcct ctttttgcga
    19681 caagtttaaa acgtgcagat tccttggcta tcgctatgga agcgcgtggc tatcagggtg
    19741 gaaaaggcag aagtcaatac cgacaattga aatggactct aaaggatacg ctgaccattc
    19801 ttgttattct cgtacttggt tgttgtttat ttttcttaaa atcttagtag ctttcaggaa
    19861 ttgataaaaa gttactgtaa cagtttttga tataaaggta gggatatgaa ccgttttaaa
    19921 aaatcaaaat atgtcattat tgtttttgtc actgttctgc ttgtgtcagc tctcttagcg
    19981 acgacttatt caagtacaat tgtgacaaaa ttaggagatg gaatctcatt ggttgataga
    20041 gttgtacaaa aaccttttca gtggtttgat tctgtcaaat cagatttggc tcatttgaca
    20101 cgaacatata atgaaaatga aagtttgaag aaacagcttt accaattaga agttaaatca
    20161 aatgaggtgg aaagtttaaa gacagaaaat gaacaactgc gccaattgct tgatatgaag
    20221 tctaaattgc aagccacaaa gactttagca gcagatgtta ttatgcgttc tccggtatct
    20281 tggaagcagg agttgacctt agatgcaggt agatcaaaag gtgcttctga gaacatgtta
    20341 gctattgcaa atggtggctt gattgggagt gtttcaaaag tagaggagaa ctctactata
    20401 gtcaaccttc tgacaaatac ggaaaatgct gataagattt ctgttaaaat tcaacatggc
    20461 tctactacaa tttatggaat tattattggc tatgacaagg aaaatgacgt tcttaaaatt
    20521 agccaattaa atagtaatag cgatattagt gcgggagata aggtgactac tggtggatta
    20581 ggaaacttta acgttgctga tattcctgtt ggtgaagtgg ttgccacaac gcatagtaca
    20641 gactatttga cacgagaagt aactgttaaa ttgagtgcag atactcataa tgtagatgtg
    20701 atagaattag tggggaattc ataatgagac agttgaagcg agttggagta tttttattgc
    20761 ttcctttctt tgttctaatt gacgcccata ttagccagct tctgggctca tttttccccc
    20821 atgtacattt ggctagtcat tttctttttc tatttctctt atttgagacg atagaagtat
    20881 cagagtatct ctacctagtc tattgttttg ttataggctt ggtttatgat gtttactttt
    20941 tccatctaat agggattaca actctcttat ttatcttatt gggagccttc cttcataaat
    21001 tgaatagtgt tattttgttg aatcgttgga caagaatgct agctatgatt gtgctgacat
    21061 tcctgtttga aatgggtagt tatcttttgg cttttatggt agggttgaca gtagatagca
    21121 tgtcgatttt tatagtctat agcttggtac cgacgatgat tttaaatttt ttatggatta
    21181 ctgtttttca atttattttt gaaaaatatt atctataaga acgacatata aatgtaacaa
    21241 aggcgtaata tttattaggc ctttttttgg tatactagta ttgtctttaa aagaaggagt
    21301 atctacgtaa tatgaagaaa aaaatcttag cgtcactttt attaagtaca gtaatggttt
    21361 ctcaagtagc tgttttaaca actgcgcatg cagaaacgac tgatgacaaa attgctgctc
    21421 aagataataa aattagtaac ttaacagcac aacaacaaga agcccaaaaa caagttgacc
    21481 aaattcagga gcaagtatca gctattcaag ctgagcagtc taacttgcaa gctgaaaatg
    21541 atagattaca agcagaatct aagaaactcg agggtgagat tacagaactt tctaaaaaca
    21601 ttgtttctcg taaccaatcg ttggaaaaac aagctcgtag tgctcaaaca aatggagccg
    21661 taactagcta tatcaatacc attgtaaact caaaatcaat tacagaagct atttcacgtg
    21721 ttgctgcaat gagtgaaatc gtatctgcaa acaacaaaat gttagaacaa caaaaggcag
    21781 ataaaaaagc tatttctgaa aaacaagtag caaataatga tgctatcaat actgtaattg
    21841 ctaatcaaca aaaattggct gatgatgctc aagcattgac tacgaaacag gcagaactaa
    21901 aagctgctga attaagtctt gctgctgaga aagcgacagc tgaaggggaa aaagcaagtc
    21961 tattagagca aaaagcagca gctgaggcag aggctcgtgc agctgcggta gcagaagcag
    22021 cttataaaga aaaacgagct agccaacaac aatcagtact tgcttcagca aacactaact
    22081 taacagctca agtgcaagca gtatctgaat ctgcagcagc acctgtccgt gcaaaagttc
    22141 gtccaacata cagtacaaac gcttcaagtt atccaattgg agaatgtaca tggggagtaa
    22201 aaacattggc accttgggct ggagactact ggggtaatgg agcacagtgg gctacaagtg
    22261 cagcagcagc aggtttccgt acaggttcaa cacctcaagt tggagcaatt gcatgttgga
    22321 atgatggtgg atatggtcac gtagcggttg ttacagctgt tgaatcaaca acacgtatcc
    22381 aagtatcaga atcaaattat gcaggtaatc gtacaattgg aaatcaccgt ggatggttca
    22441 atccaacaac aacttctgaa ggttttgtta catatattta tgcagattaa tttacagagg
    22501 gactcgaata gagccctctt ttcaggtttt accgtgacaa tccctattaa aaattatatc
    22561 aaaatagctt gaaaatattg gaaaagtatg gtaaaatgaa aattgtcgtg tgaacgataa
    22621 tactcattct tgatgaattg tgaagcagtt gcccttgggt cgttttgcga gttgaagtca
    22681 agaagaggaa aaaaacaaaa aggagaaata ctcatggcag taatttcaat gaaacaactt
    22741 cttgaggctg gtgtacactt tggtcaccaa actcgtcgct ggaatcctaa gatggctaag
    22801 tacatcttta ctgaacgtaa cggaatccac gttatcgact tgcaacaaac tgtaaaatac
    22861 gctgaccaag catacgactt catgcgtgat gcagcagcta acgatgcagt tgtattgttc
    22921 gttggtacta agaaacaagc agctgatgca gttgctgaag aagcagtacg ttcaggtcaa
    22981 tacttcatca accaccgttg gttgggtgga actcttacaa actggggaac aatccaaaaa
    23041 cgtatcgctc gtttgaaaga aattaaacgt atggaagaag atggaacttt cgaagttctt
    23101 cctaagaaag aagttgcact tcttaacaaa caacgtgcgc gtcttgaaaa attcttgggt
    23161 ggtatcgaag atatgcctcg tatcccagat gtgatgtacg tagttgaccc acataaagag
    23221 caaatcgctg ttaaagaagc taaaaaattg ggaatcccag ttgtagcgat ggttgacacc
    23281 aatactgatc cagatgatat cgatgtaatc atcccagcta acgatgacgc tatccgtgct
    23341 gttaaattga tcacagctaa attggctgac gctattatcg aaggacgtca aggtgaggat
    23401 gcagtagcag ttgaagcaga atttgcagct tcagaaactc aagcagattc aattgaagaa
    23461 atcgttgaag ttgtagaagg tgacaacaaa tagtaattac ctaggagggc ggggcttagc
    23521 ccggctctcc tattttcaaa aaatatagga gaattaaaat ggcagaaatt acagctaaac
    23581 ttgtaaaaga gttgcgtgaa aaatctggtg ccggtgttat ggacgctaaa aaagcgcttg
    23641 tagaaacaga cggtgacatc gaaaaagcga ttgaattgct tcgtgaaaaa ggtatggcta
    23701 aggcagctaa gaaagctgac cgtgttgctg cagaaggttt gactggtgtt tatgttaacg
    23761 gtaatgttgc agcagttatt gaagtaaacg ctgaaactga cttcgttgca aaaaacgctc
    23821 aattcgttga attggtaaat actacagcta aagtcattgc tgaaggaaaa cctgctaata
    23881 atgaagaagc tcttgctttg ataatgcctt caggtgaaac tcttgaagct gcatacgtat
    23941 ctgcaacagc aactatcgga gagaaaatct cattccgtcg ctttgcattg attgaaaaaa
    24001 cagacgcaca acactttgga gcataccaac ataacggtgg acgtatcggt gttatttcag
    24061 ttgttgaagg tggagacgaa gcacttgcta aacaattgtc aatgcacatc gcagcgatga
    24121 aaccaacagt tctttcttac aaagaattgg atgagcaatt cgttaaagat gagttggcac
    24181 aattgaatca cgttatcgac caagacaacg aaagccgtgc aatggttaat aaaccagctc
    24241 ttccacactt gaagtatgga tcaaaagctc aattaactga tgatgttatt gctcaagctg
    24301 aagctgacat caaagctgaa ttggctgcag aaggcaaacc agaaaaaatc tgggacaaaa
    24361 ttatcccagg taaaatggat cgcttcatgc ttgataatac taaagttgac caagcttaca
    24421 cacttcttgc acaagtttac atcatggatg acagcaagac agttgaagca taccttgaat
    24481 cagttaacgc ttcggtagtt gagtttgctc gctttgaagt tggtgaaggt atcgagaaag
    24541 ctgcaaacga cttcgaagct gaagttgcag ctacaatggc agcagccttg aataactaat
    24601 aatagaaagg aagcaaattg cttccttttt tgtatagggc ttagattttt cctgaaattg
    24661 ttttttcccc agtctcactc gattagaata attaaaacaa aaagccctat aataagggct
    24721 aagtgcattt aggagactat acttcaaatt catagtaagc gcgtcataac aaggtatcta
    24781 tcctaattcc ccaacttcca gtataccaga taaaaaataa ttatcggagg aaaggtattg
    24841 tactatgata attcaactaa gtgatttagg tcaagttcac cttgtttgtg gcaagacaga
    24901 tatgaggcag ggaatagact cattagccta tgtagttaaa acccactttg aattggatcc
    24961 tttctccgat caagtctttc tcttttgtgg tggacgtaaa gaccgcttta aagttcttta
    25021 ctgggatggt caaggatttt ggatattata taaacgcttt gagaacggaa aactgacttg
    25081 gtccagtaca gaaaaggatg tcaaagctct cacatctgaa caagtagatt ggctgatgaa
    25141 gggcttttct atcactcccc aaatatagtg gatttaaact agaatagtac acctctgctt
    25201 ctaaaacatt gttagaaatc gatttgacta tcctgttctt atttcatttt actataaatt
    25261 catcagaaag tcgtgatttc tattgaaatg aggactttct ttttatactc atctgttttc
    25321 aaaaagcatt ctagtccgtc tccgattaac gatggacttt atcacctcct tctccagtcc
    25381 ttgtatgaca tcttccaaag ttcgaaaggc tttattctta aatccacgtt tacgaatctc
    25441 tttccacact tgttcaatgg ggttcatctc tggtgtgtat ggaggaataa atgaaaagcc
    25501 aatattagtc ggaatcttta aggtacttga tttatgccat atagcattgt ccataacgag
    25561 taaaagataa tcatctggat aagcttgtga aagctcctat tcctaaagcc cctttagcgc
    25621 ataactttgg ctcagcttct attatcgctc acaccatcca tcagaagttt aatctgaagg
    25681 tacccaatta tcgccaagaa gaaggttggg ctaggatggg tttaccaatc acacgtaagg
    25741 aaattgttaa ttggcatatc aaggcgagtc aatactattt ggagcccctt tataacctct
    25801 tgcgagagag actattgacg cagtccttac ttcatgcgga tgaaacttct tatagggtgc
    25861 tagagagtga tagtcagctg acttactatt ggactttttt gtctgggaaa gctgagaaac
    25921 aagggattac gctttaccat catgtcctaa tcgatttgtt catatcttat ttcaatccat
    25981 tataaatagt gagaaatatc tatcctatct tctagaatgt cttccaaacg tggaaactct
    26041 cgtaaacaaa gaggttttag aggcctattt accatggact aaagttgtac aagaaaagtg
    26101 caaataagaa atctccagat taggaactat ccgtgagttc tctagtctgg agatttttca
    26161 atagacttcg ttattggatg gttacaattc ataaagtgct gtagagagat aacgttcacc
    26221 gttatctggt gctagggcaa ggactttttt acctgtacct aattttttgg caacctcaat
    26281 ggctccgtag atagctgcag ctgaggaaat ccctacaagg aagccttctt ttccaccaat
    26341 ttcacgtccg agtgcaagag cgtcatctga tgttacacga acgataccat cataggcttt
    26401 agtatcaagt gtatcaggaa taaatccagc tgagatacct tgaattttgt gaggaccagg
    26461 tttttcacca gatagaatag cagattcatc tgcttctact gcaaaaactt gaatgttaga
    26521 attttctgat ttgagtgcat gagaaacacc agaaatcgtt ccaccggtac ctactccagc
    26581 aacaaaggca tctaatccat ctttaccgaa agcagctagt atctcagctc ctgttgttct
    26641 ttcgtgtact tctggattag ctggattgtc aaattgaaga ggaaggaaac catcacgttc
    26701 agcagcgatt tcttgagcct tagcaatagc acctttcatt ccctcgctac caggagttag
    26761 gacgagttca gcaccataag cttggataat tttacgtcgt tctacactca tagtttcagg
    26821 cataacgatg acgactttat accctttagc agcacctacc catgaaagtc caataccagt
    26881 gtttccactt gttgcttcaa caatagtaga accaggtttc agaataccat cttgttcagc
    26941 tttttcaatc atgctaaggg caatacggtc ttttacagat gaaccaggat taaatgcttc
    27001 aagctttata tagacgtctg cagcaccttc tggcacgatg ttgttaagtt taacaatcgg
    27061 tgtttgaccg attaattcag taatgttgtt ataaatagac atatgaatac ctctttgtat
    27121 aagatatctt tagtataacg ctatccatac catttgtaaa atatagaaat cctatcgaag
    27181 cgataggatt tctatatatc atagatcaaa gtcattaatt ttcaagtgat tgcgttcagc
    27241 taattctagt aaataggtgt gaaggaaaac gatatcaggt ttcgtttgaa ttgcttcatt
    27301 ttctagtata ttttggtacg catcttttta ttttgctcgc taaagtagtt gtgagaatgt
    27361 caacgaaacg ctgtattatt cattgtttac actaagttta caggaacttc aacctctcgt
    27421 aaaccttggt cagttaaagt gacttttcca ttaaaaaact ccacaagtgc agctttaata
    27481 gtttcttttt cttctttatc aacataaatc atcgtatcga cttgatctgt aaagtttgta
    27541 tccagctcca tgagatcatg ttctttaagg aagttactgt actcttggta ctgagcataa
    27601 gacatttgaa tagcaatgcc agcctgttct tttatttcaa taataccaat ttctttgaca
    27661 gctaaggcga cactgccggc gtaagcacga attagtcctc cagcgcctag tttaatacca
    27721 ccaaagtagc gtgtcacgac cacacagaca ttggtgagat tgtgattttc tagtacccca
    27781 agcatgggaa caccagcagt accactaggc tcaccatcat cacttgtacg tttaatttca
    27841 ctacgttctc caataataaa ggcagagcag ttatgtgtcg ctttgtagtg ttcttttttg
    27901 atggcagtaa tgaagtcacg agcctcttct tcgctataaa cacgcttggc atggcagata
    27961 aagcgagatt ttttgatttc ttcttggact tgaccgtcct ctttaattgt cctaaattcc
    28021 atgatttaat tgtactaaaa aatcatctaa agtgctagtt tttacgaata aagaagtatg
    28081 aaagtaaatt tagattatct cggtcgttta tttactgaga atgaattaac agaagaagaa
    28141 cgtcagttgg cggagaaact tccagcaatg agaaaggaga aggggaaact tttctgtcaa
    28201 cgctgtaata gtactattct agaagaatgg tatttgccca tcggtgctta ctattgtcga
    28261 gagtgcttgc tgatgaagcg agtcagaagt gatcaaactt tatactattt tccgcaggag
    28321 gattttccaa agcaagatgt tctcaaatgg cgcggccaat taactccttt tcaagagaag
    28381 gtgtcagagg gattgcttca agtagtagac aagcaaaagc caaccttagt tcatgcggta
    28441 acaggagctg gaaagacaga aatgatttat caagtagtgg ctaaagtgat caatgcgggt
    28501 ggtgcagtgt gtttggctag tcctcgcata gatgtttgtt tggatctgta caagcgcctg
    28561 caacaggatt tttcttgcgg gatagctttg ctacatggag aatcggaacc ttattttcga
    28621 acaccactag ttgttgcaac aacccatcag ttattgaagt tttatcaagc ttttgatttg
    28681 ctgatagtgg atgaagtaga tgcttttcct tatgttgata atcccatgct ttaccacgct
    28741 gtcaagaata gtgtaaagga gaatggattg agaatctttt taacagcgac ttcgaccaat
    28801 gagttagata aaaaggtccg tttaggagaa ctaaaaagac tgaatttacc gagacggttt
    28861 catggaaatc cgttgattat tccaaaacca atttggttat cggattttaa tcgctactta
    28921 gacaagaatc gtttgtcacc aaagttaaag tcctatattg agaagcagag aaagacagct
    28981 tatccgttac tcatttttgc ttcagaaatt aagaaagggg agcagttagc agaaatctta
    29041 caggagcaat ttccaaatga gaaaattggc tttgtatctt ctgtaacaga ggatcgatta
    29101 gagcaagtac aagcttttcg agatggagaa ctgacaatac ttatcagtac gacaatcttg
    29161 gagcgtgaag ttaccttccc ttgtgtggat gttttcgtag tagaggccaa tcatcgtttg
    29221 tttaccaagt ctagtttgat tcagattggt ggacgagttg gacgaagcat ggatagaccg
    29281 acaggagatt tgcttttctt ccatgatggg ttaaatgctt caatcaagaa ggcgattaag
    29341 gaaattcaga tgatgaataa ggaggctggt ctatgaagtg cttgttatgt gggcagacta
    29401 tgaagactgt tttaactttt agtagtctct tacttctgag gaatgatgac tcttgtcttt
    29461 gttcagactg tgattctact tttgaaagaa ttggggaaga gaactgtcca aattgtatga
    29521 aaacagagtt gtcaacaaag tgtcaagatt gtcaactttg gtgtaaagag ggagttgaag
    29581 tcagtcatag agcgattttt acttacaatc aagctatgaa ggattttttc agtcggtata
    29641 agtttgatgg agacttcctg ttaagaaaag ttttcgcttc atttttaagt gaggagttga
    29701 aaaagtacaa agagtatcaa tttgttgtaa ttcccctaag tcctgataga tatgctaata
    29761 gaggatttaa tcaggttgag ggcttggtag aggcagcagg ctttgagtat ctggatttat
    29821 tagagaaaag agaagagaga gccagttctt ctaaaaatcg ttcagagcgc ttggggacag
    29881 aacttccttt ctttattaaa agtggagtca ctattcctaa aaaaatccta cttatagatg
    29941 atatctatac tacaggagca actataaatc gtgttaagaa actgttggaa gaagctggtg
    30001 ctaaggatgt aaaaacattt tcccttgtaa gatgaggaaa aaatttgcaa aaacagaatg
    30061 aaagcgttat aataaaatca taaaaacaaa aatgtttaga aagaaggtac tcatatgatt
    30121 aaatatagta tccgtggtga aaacctagaa gtaacagaag caattcgtga ttatgtagtt
    30181 tctaaactcg aaaagatcga aaagtacttc caaccagaac aagagttgga tgcccgaatt
    30241 aacttaaaag tttatcgtga aaaaacggct aaagtggaag taacgattcc gcttggatct
    30301 attactctcc gcgcagaaga tgtatctcaa gatatgtatg gttcaattga ccttgtaact
    30361 gataaaattg aacgtcagat tcgtaaaaat aaaacaaaaa tcgagcgtaa aaataaaaat
    30421 aaggtagcaa ctggtcaatt atttacagat gctttggtgg aagattcaaa tgttgtccag
    30481 tctaaagttg ttcgttcaaa acaaattgat ttaaaaccaa tggatttgga agaagcaatt
    30541 ctacaaatgg atttattggg gcatgatttc tttatctatg tggatgttga agatcagaca
    30601 accaatgtga tttatcgtcg tgaggatggc gaaattggtt tgttagaggt taaagaatct
    30661 taatttcaat atgtgtaaag gtaggtttac tgaattgtaa actacctttt tcttataatt
    30721 gatagaatta ctgattacac ttttaaaaag tcgctttttg aggggtatta tggtataatg
    30781 ggtgccaaga ggttttgaat gaaaaaattt tatgtaagtc caatttttcc tattctagta
    30841 ggattgattg cgtttggagt cttatccact ttcattattt ttgttaataa taatctgttg
    30901 acggttttaa ttttgtttct ttttgtagga ggctatgttt ttttatttaa gaaactgaga
    30961 gtgcattata caaggagtga tgtagaacag atacagtatg taaaccacca agcggaagaa
    31021 agtttgacat ctctattgga acaaatgcct gtaggtgtta tgaaattgaa tttatcttct
    31081 ggagaggttg agtggtttaa tccctatgct gaattgattt tgaccaagga agatggtgat
    31141 tttgatttag aagctgttca aacgattatc aaggcttcag taggaaatcc gtctacttat
    31201 gccaagcttg gtgagaagcg ttatgctgtt catatggatg cttcttccgg tgttttgtat
    31261 tttgtagatg tatccaggga acaagccata acagatgaat tggtaacaag tcgaccagtg
    31321 attgggattg tctctgtgga taattatgat gatttggagg atgcaacttc tgagtcagat
    31381 attagtcaaa tcaatagttt tgtagctaat tttatatcag agttttcaga aaaatacatg
    31441 atgttttctc gtcgggtaag tatggatcga ttttatctat ttactgacta cacggtgctt
    31501 gagggcttga tgaatgataa attttctgtt attgatactt tcagagaaga gtcgaaacag
    31561 agacagttgc ccttgacctt aagtatggga ttttcttatg gcgatggaaa tcatgatgag
    31621 atagggaaag ttgctttgct caatttgaac ttggctgaag tacgtggtgg cgaccaggtg
    31681 gttgttaagg aaaacgacga aacgaaaaat ccagtttatt ttggtggtgg gtctgctgct
    31741 tcaatcaagc gtacacggac tcgtacgcgc gctatgatga cagctatttc agataagatt
    31801 cggagtgtag atcatgtttt tgtagtcggt cacaaaaatt tagacatgga tgctttgggc
    31861 tctgctgtag gtatgcagtt gttcgccagc aatgtgattg aaaatagcta tgctctttat
    31921 gatgaagaac aaatgtctcc agatattgaa cgagctgttt cattcataga aaaagaagga
    31981 gttacgaagt tgttgtctgt taaggatgca atggggatgg tgaccaatcg ttctttgttg
    32041 attcttgtag accattcaaa gacagcctta acattatcaa aagaatttta tgatttattt
    32101 acccaaacca ttgttattga ccaccataga agggatcagg attttccaga taatgcggtt
    32161 attacttata tcgaaagtgg tgcaagtagt gccagtgagt tggtaacgga attgattcag
    32221 ttccagaatt ctaagaaaaa tcgtttgagt cgtatgcaag caagtgtctt gatggctggt
    32281 atgatgttgg atactaaaaa tttcacctcg cgagtaacta gtcggacatt tgatgttgct
    32341 agctatctca gaacgcgcgg aagtgatagt attgctatcc aggaaatcgc tgcgacagat
    32401 tttgaagaat atcgtgaggt caatgaactt attttacagg ggcgtaaatt aggttcagat
    32461 gtactaatag cagaggctaa ggacacgaaa tgctatgata cagttgttat tagtaaggca
    32521 gcagatgcca tgttagccat gtcaggtatt gaagcgagtt ttgttcttgc gaagaataca
    32581 caaggattta tctctatctc agctcgaagt cgtagtaaac tgaatgtaca acggattatg
    32641 gaagagttag gcggtggagg ccactttaat ttggcagcag ctcaaattaa agatgtaacc
    32701 ttgtcagaag caggtgaaaa actgacagaa attgtattaa atgaaataaa ggaaaaggag
    32761 aaagaagaat gaaagtaatc tttttagcag atgttaaagg aaaaggtaaa aaaggcgaaa
    32821 ttaaggaagt accaacaggg tatgcgcaaa actttcttat caaaaagaat ctagccaaag
    32881 aagcgactgc tcaagctgta ggtgaacttc gtggtaaaca aaaatcggaa gaaaaagctc
    32941 acgctgagat gattgcagaa gggaaagcaa ttaaagcaca acttgaagca gaagaaactg
    33001 ttgtagaatt tgttgaaaaa gttggtccag atggtcgtac ctttggttct attaccaata
    33061 agaagattgc agaagaatta caaaagcaat ttggaattaa gattgataaa cgtcatattc
    33121 aagtacaagc tccgattcga gcggttggtt tgattgatgt gccagtgaaa atctatcaag
    33181 atatcacaag tgtaatcaat cttcgtgtga aagaaggata agtttacacc ttcttgacaa
    33241 gattgtaaaa ggaagggaag tctgatggca gaagtagaag agttacgagt acaacctcaa
    33301 gatatcttag ctgagcaatc cgttttaggg gctatcttta ttgatgagag taaacttgtt
    33361 tttgtgcgag aatacattga gtctcgggac ttttttaagt atgcccatcg tttgattttc
    33421 caagccatgg tcgatttatc cgatcgtggt gatgccatag atgcaacaac ggttcgtact
    33481 atccttgata atcaaggtga tttacagaat attggtggct tgtcttactt ggttgagatt
    33541 gtcaattctg tgccaacttc tgctaatgcg gagtattatg ctaagattgt tgcagaaaaa
    33601 gcaatgctac gtcgtttaat tgccaagttg acagagtctg tcaaccaagc ttacgaagcg
    33661 tcacaaccag ctgatgaaat tattgctcag gcagaaaaag ggctgattga tgtcagtgaa
    33721 aatgcaaatc gaagcgggtt taagaacatt cgagatgtgt tgaatctcaa ctttggaaat
    33781 ctggaagctc gctcgcaaca aacgaccgat attacaggta ttgcgacagg ttatcgtgat
    33841 ttggatcata tgacaacagg acttcatgag gaggagttga ttatcttagc agctcgtcca
    33901 gcagttggta agacagcatt tgccttgaat atcgctcaga atattgggac taagttggac
    33961 aaaacggttg ctattttttc actcgaaatg ggtgcggaaa gcttggtaga ccgtatgtta
    34021 gctgcagaag gcttggtgga gtcacattct atccgtacag ggcaattgac agatgaggag
    34081 tggcaaaaat atactattgc tcagggtaat ctagctaacg ccagtatcta tatcgatgat
    34141 acgccaggta ttcggattac agagattcgt tctcgttctc gtaaattggc tcaagaaact
    34201 ggaaatcttg gtttgatttt gatagactat ttgcaactta tcacgggaac tggtcgagaa
    34261 aatcgtcaac aagaagtttc tgaaatttct cgtcagttga aaatactagc caaggaattg
    34321 aaggttccag taatcgctct gagtcagctt tctcgtggtg tagaacaacg tcaggacaag
    34381 agaccggtct tgtctgatat tcgtgaatct gggtctattg agcaggacgc tgatatcgta
    34441 gcttttctct atcgcgatga ctactatgaa cgtggtggtg aagaagagga gggtatccca
    34501 aataataagg tggaagttat tatcgagaaa aaccgtagtg gagctcgtgg aacagtggaa
    34561 ttgattttcc aaaaagaata caataaattt tcaagtatct caaagaggga ggcataagat
    34621 gtcagatgca tttacagatg tagccaagat gaaaaaaatc aaagaagaaa tcaaggcaca
    34681 tgagggacaa gtcgtagaaa tgactttgga gaatggtcgt aagcgccaaa aaaatagatt
    34741 gggtaagcta attgaagttt atccatccct atttattgtg gagtttgggg atgtggaagg
    34801 agataaacaa gttaatgttt acgttgaatc ctttacttac tcagatattc ttacagaaaa
    34861 gaatttgatt cattatcttg actaaagtga gaaattttct cactttttct tttttctccg
    34921 aataatttag gtgaaggcaa tcatcgcttt atattatttt ttaaggagga agaatgaaaa
    34981 ttttaccgtt tatagcaaga ggaacaagtt attacttgaa gatgtcagtt aaaaagcttg
    35041 ttcctttttt agtagtagga ttgatgctag cagctggtga tagtgtctat gcctattccg
    35101 gaggaaatgg atcgattgcg cgtggggatg attatcctgc ttattataaa aatgggagcc
    35161 aggagattga tcagtggcgc atgtattctc gtcagtgtac ttcttttgta gcctttcgtt
    35221 tgagtaatgt caatggtttt gaaattccgg cagcttatgg aaatgcgaat gaatggggac
    35281 atcgtgctcg tcgggaaggt tatcgtgtag ataatacacc gatgattggt tccattactt
    35341 ggtctactgc aggaacttat ggtcatgttg cctgggtgtc aaatgtaatg ggagatcaga
    35401 ttgagattga ggaatataac tatggttata cagaatccta taataaacga attataaaag
    35461 caaacacgat gacaggattt attcatttta aagatttgga tggtggcagt gttgggaata
    35521 gtcaatcctc aacttcaaca ggcggaactc attattttaa gaccaagtct gctattaaaa
    35581 ctgaacccct agttagtgca actgtgattg attactatta tcctggagag aaggttcatt
    35641 atgatcaaat tctcgaaaaa gacggctaca agtggttgag ttatactgcc tataatggaa
    35701 gctatcgtta tgttcaattg gaggctgtga ataaaaatcc tctaggtaat tctgttcttt
    35761 cttcaacagg tggaactcat tattttaaga ccaagtctgc tatcaaaact gaacccctag
    35821 ttagtgcaac tgtgattgat tactattatc ctggggagaa ggttcattat gatcagatac
    35881 ttgaaaaaga cggctacaag tggttgagtt atacggctta taacggaagt cgtcgctata
    35941 tacagctaga gggagtgact tcttcacaaa attatcagaa tcaatcagga aatatctcta
    36001 gctatggatc caataatagt tcaactgtcg gttggaagaa aataaatggt agttggtatc
    36061 atttcaaatc aaatggttct aaatcaacag gatggctgaa agatggttct agctggtatt
    36121 atttgaaatt atctggtgaa atgcagacag gatggttaaa ggagaatggc tcgtggtatt
    36181 atctgggtag ttcaggggca atgaaaacag gctggtacca ggtctctggt aagtggtatt
    36241 attcttactc ttcaggcgcc ttagctgtta atacgacggt ggatggctac agagtaaaca
    36301 gtgatggaga acgagtatag aaaattggag taggagaaat ttcctgctct tttctgtaag
    36361 cagtttcctt gacatttttc ttattttgcg ctatcatata tacatataat atatgggagt
    36421 ctgtgtacag tctgagagga agtgttaaac ttcgaccgca cctgatctgg gtaatgccag
    36481 cggagggaac gatacttagt ctaattttgc accttttcca tgtatggtaa aggtttttct
    36541 tttttttaaa aggaaaacga gaagaggagg ttcttatgaa agcaagcatt gccttgcaag
    36601 ttttacccct agtacagggg attgatcgga tagctgttat tgatcaggtc attgcttatc
    36661 tgcaaactca agaagtgccg atggtagtga caccatttga aacggtcttg gaaggggagt
    36721 ttgatgagct tatgcgcatt ctaaaagaag cgctggaagt ggcagggcag gaggcagaca
    36781 atgtctttgc caatgtcaaa ataaatgtag gagagatttt aagtattgat gagaaacttg
    36841 agaagtatac tgagacgaca cattagtcta ttgggctttc tcggagtctt gtcaatctgg
    36901 cagttagcag gttttcttaa actgctcccc aagtttatcc tgccgacacc tcttgaaatt
    36961 ctccagccct ttgttcgtga cagagaattt ctctggcacc atagctgggc gaccttgaga
    37021 gtggctttac tggggctgat tttgggagtt ttgattgcct gtcttatggc tgtactcatg
    37081 gatagtttga cttggctcaa tgacctgatt taccctatga tggtggtcat tcagaccatt
    37141 ccgaccattg ccatagctcc tatcctggtc ttgtggctag gttatgggat tttgcccaag
    37201 attgtcttga ttatcttaac gacaaccttt cccatcatcg tcagcatttt ggacggtttt
    37261 aggcattgcg acaaggatat gctgactttg tttagtctga tgcgggccaa gccttggcaa
    37321 atcctgtggc attttaaaat tccagtcagc ctgccctact tttatgctgg tctgagggtc
    37381 agtgtctcct acgcttttat cacaacagtg gtatctgagt ggttgggagg ctttgagggg
    37441 cttggtgttt atatgattca gtccaagaaa ttgtttcagt atgataccat gtttgccatt
    37501 attattctgg tgtcgattat cagtcttttg ggtatgaagc tggtcgatat cagtgaaaaa
    37561 tatgtgatta aatggaaacg tacttaaaat agagcgtttt tgaaaaagaa aagaggaaat
    37621 caaaatgaag aaaacatgga aagtgttttt aacgcttgta acagctcttg tagctgttgt
    37681 gcttgtggcc tgtggtcaag gaactgcttc taaagacaac aaagaggcag aacttaagaa
    37741 ggttgacttt atcctagact ggacaccaaa taccaaccac acagggcttt atgttgccaa
    37801 ggaaaaaggt tatttcaaag aagctggagt ggatgttgat ttgaaattgc caccagaaga
    37861 aagttcttct gacttggtta tcaacggaaa ggcaccattt gcagtgtatt tccaagacta
    37921 catggctaag aaattggaaa aaggagcagg aatcactgcc gttgcagcta ttgttgaaca
    37981 caatacatca ggaatcatct ctcgtaaatc tgataatgta agcagtccaa aagacttggt
    38041 tggtaagaaa tatgggacat ggaatgaccc aactgaactt gctatgttga aaaccttggt
    38101 agaatctcaa ggtggagact ttgagaaggt tgaaaaagta ccaaataacg actcaaactc
    38161 aatcacaccg attgccaatg gcgtctttga tactgcttgg atttactacg gttgggatgg
    38221 tatccttgct aaatctcaag gtgtagatgc taacttcatg tacttgaaag actatgtcaa
    38281 ggagtttgac tactattcac cagttatcat cgcaaacaac gactatctga aagataacaa
    38341 agaagaagct cgcaaagtca tccaagccat caaaaaaggc taccaatatg ccatggaaca
    38401 tccagaagaa gctgcagata ttctcatcaa gaatgcacct gaactcaagg aaaaacgtga
    38461 ctttgtcatt gaatctcaaa aatacttgtc aaaagaatac gcaagcgaca aggaaaaatg
    38521 gggtcaattt gacgcagctc gctggaatgc tttctacaaa tgggataaag aaaatggtat
    38581 ccttaaagaa gacttgacag acaaaggctt caccaacgaa tttgtgaaat aatgacagaa
    38641 attagactag agcacgtcag ttatgcctat ggtcaggaga ggattttaga ggatatcaac
    38701 ctacaggtga cttcaggcga agtggtttcc atcctaggcc caagtggtgt tggaaagacc
    38761 accctcttta atctaatcgc tgggatttta gaagttcagt cagggagaat tgtccttgat
    38821 ggtgaagaaa atcccaaggg gcgcgtgagt tatatgttgc aaaaggatct gctcttggag
    38881 cacaagacgg tgcttggaaa tatcattctg cccctcttga ttcaaaaggt ggataaggca
    38941 gaagctattt cccgagcgga taaaattctt gcgaccttcc agctgacagc tgtaagagac
    39001 aagtatcctc atgaacttag cggtgggatg cgccagcgtg tagccttact ccggacctac
    39061 ctttttgggc acaagctctt tctcttagat gaggccttta gcgccttgga tgagatgaca
    39121 aagatggaac tccacgcttg gtatcttgag attcacaagc agttgcagct aacaaccctg
    39181 atcatcacgc atagtattga ggaggccctc aatctcagcg accgcatcta tatcttgaaa
    39241 aatcgccctg ggcagattgt ttcagaaatt aaactagatt ggtctgaaga tgaggacaag
    39301 gaagtccaaa agattgccta caaacgtcaa attttggcgg aattaggctt agataagtag
    39361 aaaaataggg agttggtgaa gattatcctt taccagcgcc ctttttcttt taaaaatgag
    39421 aaaatttcgg tataatagtc aaacaaggtc aaggtttaaa gagagaggtg ggtttgttat
    39481 gagatttaaa aatacatcgg atcatattga ggcctacatc aaggcgattt tagatcaatc
    39541 tggtatcgtg gagttgcaac ggagtcagtt ggcagatacc tttcaggttg ttcctagtca
    39601 gattaactac gtgatcaaga cacgctttac ggaaagtaga ggctacttgg ttgaaagtaa
    39661 gcgtggtggc ggaggctaca ttcgtatagg acggattgag ttttctagtc atcatgaaat
    39721 gctccgggag ctgctttact cgattggtga gcgagtcagt caagaaattt atgaggatat
    39781 tctccagctt ttggttgagc aggaattgat gaccaagcag gagatgaatt tgctagaatc
    39841 agtagctttg gatcgcgttt taggagaaga agctccagtt gttcgagcaa acatgctacg
    39901 tcagatcata caagaggtag atagaaaagg gaagtaagat gaactattca aaagcattga
    39961 atgaatgtat cgaaagtgcc tacatggttg ctggacattt tggagctcgt tatctagagt
    40021 cttggcactt gttgattgcc atgtctaatc acagttatag tgtagcaggg gcaactttaa
    40081 atgattatcc gtatgagatg gaccgtttag aagaggtggc tttggaactg actgaaacgg
    40141 actatagcca ggatgaaacc tttacggaat tgccgttctc ccgtcgtttg caggttcttt
    40201 ttgacgaagc agagtatgta gcgtcagtgg tccatgctaa ggtgctaggg acagagcacg
    40261 tcttctatgc gattttgcat gatggcaatg ccttggcaac tcgtatcttg gagagggctg
    40321 gtttttctta tgaagacaag aaagatcagg tcaagattgc tgctcttcgt cgaaatttag
    40381 aagaacgggc aggctggact cgtgaagatc tcaaggcttt acgccaacgc catcgtacag
    40441 tagctgacaa gcaaaattct atggccaata tgatgggcat gccgcagact cctagtggtg
    40501 gtctcgagga ctatacgcat gatttgacag agcaagcgcg ttctggcaag ttagaaccag
    40561 tcatcggtcg ggacaaggaa atctcacgta tgattcaaat cttgagccgg aagactaaga
    40621 acaaccctgt cttggttggg gatgctggtg tcgggaaaac agctctggcg cttggtcttg
    40681 ctcagcgtat tgctagtggt gacgtgcctg cggaaatggc taagatgcgc gtgttagaac
    40741 ttgatttgat gaatgtcgtt gcagggacac gcttccgtgg tgactttgaa gaacgcatga
    40801 ataatatcat caaggatatt gaagaagatg gccaagtcat cctctttatc gatgaactcc
    40861 acaccatcat gggttctggt agcggaattg attcgactct ggatgcggcc aatatcttga
    40921 aaccagcctt ggcgcgtgga actttgagaa cggttggtgc caccactcag gaagaatatc
    40981 aaaaacatat cgaaaaagat gcggcacttt ctcgtcgttt cgctaaagtg acgattgaag
    41041 aaccaagtgt ggcagatagt atgactattt tacaaggttt gaaggcgact tatgagaaac
    41101 atcaccgtgt acaaatcaca gatgaagcgg ttgaaacagc ggttaagatg gctcatcgtt
    41161 atttaaccag tcgtcacttg ccagactctg ctatcgatct cttggatgag gcggcagcaa
    41221 cagtgcaaaa taaatcaaag catgtaaaag cagacgattc tgacttgagt ccagctgaca
    41281 aggccttgat ggatggcaag tggaaacagg cagcccagct aatcgcaaaa gaagaggaag
    41341 tgcctgtcta taaagacttg gtgacagagt ctgatatttt gaccaccttg agtcgcttgt
    41401 caggaatccc agttcaaaaa ctgactcaaa cggatgctaa gaagtattta aatcttgaag
    41461 cagaactcca taaacgggtt atcggtcaag atcaagctgt ttcaagtatt agtcgtgcca
    41521 ttcgccgcaa tcagtcagga attcgcagtc acaagcgtcc gatcggttcc tttatgttcc
    41581 taggacccac aggtgtcggt aagaccgagt tggccaaggc tttggcagaa gttctctttg
    41641 atgacgaatc agcccttatc cgctttgata tgagtgagta tatggagaaa ttcgcagcca
    41701 gccgtctaaa tggagctcct ccaggctatg tgggttacga agaaggtggg gagttgacag
    41761 agaaggttcg caataaaccc tattccgttc tcctctttga tgaggtagag aaggcccatc
    41821 cagatatctt taatgttctc ttgcaggttc tggatgacgg tgtcttgaca gatagcaagg
    41881 gacgcaaggt cgatttttca aataccatta tcattatgac atcgaactta ggtgcgactg
    41941 cccttcgtga tgataagact gttggttttg gggctaagga tattcgtttt gaccaggaaa
    42001 atatggaaaa acgcatgttt gaagaactga aaaaagctta tagaccggaa ttcatcaacc
    42061 gtattgatga gaaggtggtc ttccatagcc tatctagtga ccatatgcag gaagtggtga
    42121 agattatggt caagccttta gtggcaagtt tggctgaaaa aggcattgac ttgaaattac
    42181 aagcttcagc tctgaaattg ttagcaaatc aaggatatga cccagagatg ggagctcgcc
    42241 cacttcgcag aaccctgcaa acagaagtgg aggacaagtt ggcagaactt cttctcaagg
    42301 gagatttagt ggcaggcagc acacttaaga ttggtgtcaa agcaggccag ttaaaatttg
    42361 atattgcata aaagaataaa agtatcagca tctgaccata agtcacagtg gagtgaaact
    42421 caatgaaaat caaagagcaa actaggcagc tagccgcagg ttgctcaaaa cactgttttg
    42481 aggttgcaga tagagctgac gtggtttgaa gagattttcg aagagtatga aactaaaacc
    42541 tatagcttct aaacgatccg tggttttcac cattcaacac aaaattcata tgtttattac
    42601 cctccgtcgt atttgtctta gagcgtgtgt agtagaaaaa gagcagtctt atctgaaatt
    42661 tttattcttt caaaagagac ctgtttcttt tttgcatgtc aaatccgttc tagctggtat
    42721 ttgaaaaatc aaactaatac tcaatgaaaa tcaaagaaca aactaggaag ctagccgcag
    42781 gttgctcaaa acactgtttt gaggttgtag atagagctga cgtagtttga agagattttc
    42841 gaagagtata agctgcaaga tgaatgattt tcttgtattg acgttgttgt tgacaaaaag
    42901 tagcggataa atgaaatcca ttccattatc atagatgata ggctggtagg aaattttcaa
    42961 atagcataca ggaaatagat gtatggagtt ctggtagtag aaagggagag agatgaacat
    43021 tttagttgca gatgacgagg aaatgattag agaaggaatt gcagcatttc tgacagaaga
    43081 gggttatcat gtcattatgg ctaaggatgg acaagaggtc ttggaaaaat ttcaagatct
    43141 ccctatccat ctcatggtac tggatttaat gatgcctagg aagagtggtt ttgaagtgtt
    43201 aaaagaaatc aatcaaaagc acgatattcc tgtcatcgtc ttgagtgctc tgggagatga
    43261 aactactcag tcacaggtat ttgatctcta tgctgatgat catgtgacaa aacctttttc
    43321 tttggtactg cttgtcaagc gtattaaggc gcttatcaga cgttactacg tcatagagga
    43381 tctttggcga tatcaggatg taacagtgga ttttacctct tacaaagcac attataaaaa
    43441 tgaagaaatt gatctcaaac caaaggaatt actggtacta aagtgtttga ttcagcataa
    43501 aaatcaagtt ttaagtagag agcagatatt ggaagaaatt tcaaaagatg tagctgattt
    43561 accttgtgat agggtcgttg atgtctatat tcgtactctt cgcaaaaaat tagctttaga
    43621 ttgtatcgtg actgtgaaaa atgttgggta taagattagc ttatgataaa aaatcctaaa
    43681 ttattaacca agtctttttt aagaagtttt gcaattctag gtggtgttgg tctagtcatt
    43741 catatagcta tttatttgac ctttcctttt tattatattc aactggaggg ggaaaagttt
    43801 aatgagagcg caagagtgtt tacggagtat ttaaagacta agacatctga tgaaattcca
    43861 agcttactcc agtcttattc aaagtccttg accatatctg ctcaccttaa aagagatatt
    43921 gtagataagc ggctccctct tgtgcatgac ttggatatta aagatggaaa gctatcaaat
    43981 tatatcgtga tgttagatat gtctgttagt acagcagatg gtaaacaggt aaccgtgcaa
    44041 tttgttcacg gggtagatgt ctacaaagaa gcaaagaata ttttgctttt gtatctccca
    44101 tatacatttt tggttacaat tgctttttcc tttgtttttt cttattttta tactaaacgc
    44161 ttgctcaatc ctctttttta catttcagaa gtgactagta aaatgcaaga tttggatgac
    44221 aatattcgtt ttgatgaaag taggaaagat gaagttggtg aagttggtga agttggaaaa
    44281 cagattaatg gtatgtatga gcacttgttg aaggttattc atgagttgga aagtcgtaat
    44341 gagcaaattg taaaattgca aaatcaaaag gtttcctttg tccgcggagc atcacatgag
    44401 ttgaaaaccc ctttagccag tcttagaatt atcctagaga atatgcagca taatattgga
    44461 gattacaaag atcatccaaa atatattgca aagagtataa ataagattga ccagatgagc
    44521 cacttattag aagaagtact ggagtcttct aaattccaag agtggacaga gtgtcgtgag
    44581 accttgactg ttaagccagt tttagtagat attttatcac gttatcaaga attagctcat
    44641 tcaataggtg ttacaattga aaatcaattg acagatgcta ccagggtcgt catgagtctt
    44701 agggcattgg ataaggtttt gacaaacctg attagtaatg caattaaata ttcagataaa
    44761 aatgggcgcg taatcatatc cgagcaagat ggctatctct ctatcaagaa tacatgtgcg
    44821 cctctaagtg accaagaact agaacattta tttgatatat tctatcattc tcaaatcgtg
    44881 acagataagg atgaaagttc cggtttgggt ctttacattg tgaataatat tttagaaagc
    44941 tatcaaatgg attatagttt tctcccttat gaacacggta tggaatttaa gattagcttg
    45001 tagacagatt agttttttat taaagttcat atagggttaa cataagtgta ttattcttta
    45061 tatagataaa aatatagata aaagaaagga tactaatatg gtattagcga ttattttagt
    45121 aacattcctt attcgattga tttttttaaa gcgttcgata gagaatgaga aacgaatcct
    45181 tagcaatggc gggaaagaat ttggagttga gaatacaaaa cgattaacgt tggctcatat
    45241 tgttttttat ctctcttgcc tcgttgaagc aatggtgcat aagacaatgt ttgatggtat
    45301 gagcatggtt ggtttagtct tgcttatttt ttctatgctg atgttgatgt tggtgattca
    45361 cttgttggga gatatttgga cagtgaagct tatgcttgtc aataatcaca aatatgtaga
    45421 tcatatcttg tttaggacag taaaacatcc taattacttt ttaaatattt tacctgagtt
    45481 gattggcttg accttgttga gtcatgccta tatgactttt gttttagttt ttccagttta
    45541 tgcagttatt ttgtatcgac gaatagctga agaggaaaag ctattacatg aagttataat
    45601 cccaaatgga agcataaaga gataaataca aaattcgatt tatatacagt tcatattgaa
    45661 gtgatatagt aaggttaaag aaaaaatata gaaggaaata aacatgtttg catcaaaaag
    45721 cgaaagaaaa gtacattatt caattcgtaa atttagtatt ggagtagcta gtgtagtagt
    45781 tgctagtctt gttatgggaa gtgtggttca tgcgacggag aatgagggaa ttacccaagt
    45841 agccacttct tataataagg caaatgaaag tcagacagaa cataggaaag ctgctaaaca
    45901 agtcgatgaa gatataaaaa aaatgttgag tgagatccaa gaatatataa aaaaaatgtt
    45961 gagtgagatc caattagata aaagaaaaga tacccaaaat cgcaccttaa acagaaagtt
    46021 gagcgcaatt caaacgaagt atttgtatga attaagagtt ttaaaagaga agtcgaaaaa
    46081 agaagagttg acgtcaaaaa caaaaaaaga gttagacgca gcttttgaga agtttaaaaa
    46141 agaaccagaa cttactaaaa aattagcaga agctaaacaa aaagccaagg ctcaaaaaga
    46201 agaagatttc cgtaactacc caaccaatac ttacaaaacg cttgaacttg aaattgctga
    46261 gttcgatgtg aaagttaaag aagcggagct tgaactagta aaagaggaag ctaaaccccg
    46321 aaacgaggaa aaaattaagc aagcaaaaga gaaagttgag agtaaaaaag ctgaggctac
    46381 aaggttagaa gaaatcaaga cagaacgtaa aaaagcagaa gaagaagcta aacgaaaagc
    46441 agaagaatct gagaaaaaag ctgctgaagc caaacaaaaa gtggatacta aagagcaagg
    46501 taaaccaaag aggcgggcaa aacgaggagt ttctggagag ctagcaacac ctgataaaaa
    46561 agaaaatgat gcgaagtctt cagattctag cgtaggtgaa gaaactcttc caagcccatc
    46621 ccttaatatg gcaaatgaaa gtcagacaga acataggaaa gatgtcgatg aatatataaa
    46681 aaaaatgttg agtgagatcc aattagatag aagaaaacat acccaaaatg tcaacttaaa
    46741 cataaagttg agcgcaatta aaacgaagta tttgtatgaa ttaagtgttt taaaagagaa
    46801 ctcgaaaaaa gaagagttga cgtcaaaaac caaagcagag ttaaccgcag cttttgagca
    46861 gtttaaaaaa gatacattga aaccagaaaa aaaggtagca gaagctgaga agaaggttga
    46921 agaagctaag aaaaaagcca aggatcaaaa agaagaagat cgccgtaact acccaaccaa
    46981 tacttacaaa acgcttgaac ttgaaattgc tgagtccgat gtgaaagtta aagaagcgga
    47041 gcttgaacta gtaaaagagg aagctaacga atctcgaaac gaggaaaaaa ttaagcaagc
    47101 aaaagagaaa gttgagagta aaaaagctga ggctacaagg ttagaaaaaa tcaagacaga
    47161 tcgtaaaaaa gcagaagaag aagctaaacg aaaagcagaa gaatctgaga aaaaagctgc
    47221 tgaagccaaa caaaaagtgg atgctgaaga atatgctctt gaagctaaaa tcgctgagtt
    47281 ggaatatgaa gttcagagac tagaaaaaga gctcaaagag attgatgagt ctgactcaga
    47341 agattatctt aaagaaggcc tccgtgctcc tcttcaatct aaattggata ccaaaaaagc
    47401 taaactatca aaacttgaag agttgagtga taagattgat gagttagacg ctgaaattgc
    47461 aaaacttgaa gttcaactta aagatgctga aggaaacaat aatgtagaag cctactttaa
    47521 agaaggttta gagaaaacta ctgctgagaa aaaagctgaa ttagaaaaag ctgaagctga
    47581 ccttaagaaa gcagttgatg agccagaaac tccagctccg gctcctcaac cagctccagc
    47641 tccagaaaaa ccagctgaaa aaccagctcc agctccagaa aaaccagttg aaaaaccagc
    47701 tccagctcca gaaaaaccag ttgaaaaacc agctccagct ccagctccag aaaaaccagc
    47761 tccagctcca gaaaaaccag ctccaactcc agaaactcca aaaacaggct ggaaacaaga
    47821 aaacggtatg tggtacttct acaatactga tggttcaatg gcaacaggct ggctccaaaa
    47881 caatggctca tggtactacc tcaacagcaa tggcgctatg gcgacaggat ggctccaaaa
    47941 caatggctca tggtactacc tcaacagcaa tggcgctatg gcgacaggat ggc
//
LOCUS       DAVKQB010000008        47428 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKQB010000008 DAVKQB010000000
VERSION     DAVKQB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.008"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            45..392
                     /locus_tag="VW528_000456"
     CDS             45..392
                     /locus_tag="VW528_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9911331.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDSFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEIEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(509..1642)
                     /locus_tag="VW528_000457"
                     /pseudo
     CDS             complement(509..1642)
                     /locus_tag="VW528_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1707..1988
                     /locus_tag="VW528_000458"
     CDS             1707..1988
                     /locus_tag="VW528_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9911332.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWS
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            2120..3064
                     /gene="manA"
                     /locus_tag="VW528_000459"
     CDS             2120..3064
                     /gene="manA"
                     /locus_tag="VW528_000459"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9911333.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(3085..4364)
                     /locus_tag="VW528_000460"
                     /pseudo
     CDS             complement(3085..4364)
                     /locus_tag="VW528_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            4687..5427
                     /locus_tag="VW528_000461"
     CDS             4687..5427
                     /locus_tag="VW528_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9911334.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSETAQYTSDAIAVYVQTNAE"
     gene            complement(5456..5911)
                     /locus_tag="VW528_000462"
     CDS             complement(5456..5911)
                     /locus_tag="VW528_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9911335.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(5931..7106)
                     /locus_tag="VW528_000463"
     CDS             complement(5931..7106)
                     /locus_tag="VW528_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9911336.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(7165..8010)
                     /locus_tag="VW528_000464"
     CDS             complement(7165..8010)
                     /locus_tag="VW528_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9911337.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            8135..8692
                     /locus_tag="VW528_000465"
     CDS             8135..8692
                     /locus_tag="VW528_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9911338.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFRETRKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            8711..9178
                     /locus_tag="VW528_000466"
     CDS             8711..9178
                     /locus_tag="VW528_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9911339.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            9267..9896
                     /gene="upp"
                     /locus_tag="VW528_000467"
     CDS             9267..9896
                     /gene="upp"
                     /locus_tag="VW528_000467"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9911340.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            10070..10660
                     /gene="clpP"
                     /locus_tag="VW528_000468"
     CDS             10070..10660
                     /gene="clpP"
                     /locus_tag="VW528_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9911341.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            10739..10987
                     /locus_tag="VW528_000469"
     CDS             10739..10987
                     /locus_tag="VW528_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9911342.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            11088..12248
                     /locus_tag="VW528_000470"
     CDS             11088..12248
                     /locus_tag="VW528_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9911343.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            12516..13385
                     /locus_tag="VW528_000471"
     CDS             12516..13385
                     /locus_tag="VW528_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9911344.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            13389..14345
                     /locus_tag="VW528_000472"
     CDS             13389..14345
                     /locus_tag="VW528_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9911345.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGTLLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            14345..15109
                     /locus_tag="VW528_000473"
     CDS             14345..15109
                     /locus_tag="VW528_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911346.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            15109..15819
                     /locus_tag="VW528_000474"
     CDS             15109..15819
                     /locus_tag="VW528_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911347.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            16127..16783
                     /locus_tag="VW528_000475"
     CDS             16127..16783
                     /locus_tag="VW528_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9911348.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            16891..17986
                     /gene="prfB"
                     /locus_tag="VW528_000476"
     CDS             join(16891..16962,16964..17986)
                     /gene="prfB"
                     /locus_tag="VW528_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9911349.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSYEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            18004..18696
                     /gene="ftsE"
                     /locus_tag="VW528_000477"
     CDS             18004..18696
                     /gene="ftsE"
                     /locus_tag="VW528_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9911350.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            18689..19615
                     /locus_tag="VW528_000478"
     CDS             18689..19615
                     /locus_tag="VW528_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9911351.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            19901..22081
                     /locus_tag="VW528_000479"
     CDS             19901..22081
                     /locus_tag="VW528_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9911352.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            22133..22948
                     /locus_tag="VW528_000480"
     CDS             22133..22948
                     /locus_tag="VW528_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9911353.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYNRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            23088..24431
                     /locus_tag="VW528_000481"
     CDS             23088..24431
                     /locus_tag="VW528_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9911354.1"
                     /translation="MSFTKFQFKNYIREALEELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLEIVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDRNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            24808..25998
                     /locus_tag="VW528_000482"
     CDS             24808..25998
                     /locus_tag="VW528_000482"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9911355.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            26551..27486
                     /locus_tag="VW528_000483"
     CDS             26551..27486
                     /locus_tag="VW528_000483"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9911356.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            27520..28557
                     /locus_tag="VW528_000484"
     CDS             27520..28557
                     /locus_tag="VW528_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9911357.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            28730..29335
                     /locus_tag="VW528_000485"
     CDS             28730..29335
                     /locus_tag="VW528_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9911358.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            29492..30022
                     /locus_tag="VW528_000486"
     CDS             29492..30022
                     /locus_tag="VW528_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9911359.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            30043..31128
                     /gene="rlmN"
                     /locus_tag="VW528_000487"
     CDS             30043..31128
                     /gene="rlmN"
                     /locus_tag="VW528_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU9911360.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFVCTINDDKGMAIGARHITISTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            31128..31610
                     /locus_tag="VW528_000488"
     CDS             31128..31610
                     /locus_tag="VW528_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9911361.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            31612..33153
                     /locus_tag="VW528_000489"
     CDS             31612..33153
                     /locus_tag="VW528_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9911362.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(33209..34012)
                     /locus_tag="VW528_000490"
     CDS             complement(33209..34012)
                     /locus_tag="VW528_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9911363.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(34095..34316)
                     /locus_tag="VW528_000491"
     CDS             complement(34095..34316)
                     /locus_tag="VW528_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911364.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            complement(34795..34992)
                     /locus_tag="VW528_000492"
     CDS             complement(34795..34992)
                     /locus_tag="VW528_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911365.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKRNMDLLADD
                     SLAGDIILLWRIQFGTFTTET"
     gene            35162..35434
                     /gene="rpsP"
                     /locus_tag="VW528_000493"
     CDS             35162..35434
                     /gene="rpsP"
                     /locus_tag="VW528_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9911366.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            35454..35693
                     /locus_tag="VW528_000494"
     CDS             35454..35693
                     /locus_tag="VW528_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9911367.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            36270..37058
                     /locus_tag="VW528_000495"
     CDS             36270..37058
                     /locus_tag="VW528_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9911368.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELLE"
     gene            37089..37607
                     /gene="rimM"
                     /locus_tag="VW528_000496"
     CDS             37089..37607
                     /gene="rimM"
                     /locus_tag="VW528_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9911369.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            37597..38316
                     /gene="trmD"
                     /locus_tag="VW528_000497"
     CDS             37597..38316
                     /gene="trmD"
                     /locus_tag="VW528_000497"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9911370.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            38328..38666
                     /locus_tag="VW528_000498"
     CDS             38328..38666
                     /locus_tag="VW528_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911371.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(38697..39017)
                     /locus_tag="VW528_000499"
     CDS             complement(38697..39017)
                     /locus_tag="VW528_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911372.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            39115..39330
                     /locus_tag="VW528_000500"
     CDS             39115..39330
                     /locus_tag="VW528_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9911373.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(39489..39575)
                     /locus_tag="VW528_000501"
                     /pseudo
     CDS             complement(39489..39575)
                     /locus_tag="VW528_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(39626..40162)
                     /locus_tag="VW528_000502"
     CDS             complement(39626..40162)
                     /locus_tag="VW528_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9911374.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            complement(40359..41705)
                     /gene="gor"
                     /locus_tag="VW528_000503"
     CDS             complement(40359..41705)
                     /gene="gor"
                     /locus_tag="VW528_000503"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9911375.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGLYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            41991..43190
                     /locus_tag="VW528_000504"
     CDS             41991..43190
                     /locus_tag="VW528_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9911376.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            43174..43875
                     /locus_tag="VW528_000505"
     CDS             43174..43875
                     /locus_tag="VW528_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911377.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            43877..45136
                     /locus_tag="VW528_000506"
     CDS             43877..45136
                     /locus_tag="VW528_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9911378.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            45260..47257
                     /gene="metG"
                     /locus_tag="VW528_000507"
     CDS             45260..47257
                     /gene="metG"
                     /locus_tag="VW528_000507"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9911379.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLPLLTVDPAVPNGSVIG"
BASE COUNT        14489 a         8795 c        10286 g        13858 t
ORIGIN      
        1 tttaaaagat cggagcaatc cggtcttttt atggaggaga gtaaatgaat ttaaatcaat
       61 tagatattat cgtttcaaat gttccccaag tctgtgctga cttggagcat atcttggata
      121 aaaaggcaga ttatgctgat gatagttttg ctcagtttac gattggtagt cactgcctta
      181 tgttgtcaca aaatcatttg gttcctttgg aaaactttca gtcaggaatc attattcata
      241 tcgagattga ggatgtagac cagaactaca aacggttgaa cgagcttggt ataaaggttt
      301 tacacggacc aactgtaacc gattggggaa cagagtcctt attagttcaa ggacctgctg
      361 gtctagtgct tgatttttat cgtatgaaat aggtcgcgta gtcatttgac gttaacctta
      421 gctattttta aaatagaaat tgagaatatg aactattgaa agatcggagc aatccggtct
      481 ttttatataa agaaattgca tataatactc atctgctttc aaaaagcatt ctagtccatc
      541 tccgattaac gatggacttt atcacctcct tctccaatcc ttgtatgaca tcttggagtt
      601 gattcatgac atcttccaaa gttcgaaagg ctttattctt aaatccacgt ttacgaatct
      661 ctttccacac ttgttcaatg gggttcatct ctggtgtgta tggaggaata aatgcaaagc
      721 caatattagt cggaatcttt aaggtacttg atttatgcca tatagcattg tccataacga
      781 gtaaaaaata atcatatgga taagcttgtg aaagctcttc taaaaagacg ttcatccact
      841 cagtattaca tccaccagct cttaagaaaa atgattcgcc tgtataggca tcaacagctc
      901 cataacaata gcgaaattct cgtatatagt gactatggat atgtggacct actcctattg
      961 gagaccaaca agatcccagt ttactgattc taccgaaacc agcctcatct tggtacatca
     1021 agcgaacctt acgaaaacgt ctactggttt taaatcgctt tcttttcttc ttgaattgag
     1081 attttatttt tagacgcgac aatggtttga gcgtctgctt tcttaggatg ttctggacgt
     1141 ggcgtaatat ttcgccaacc atggcgcttc aacagttgat agaaggcatc acgtgtgtag
     1201 gaacgaccta actccttttt ataagcctga aataaggcat caattgtaac aaattctcct
     1261 gcctctgtag ccttcaaatg acgggcaaga aaggctttct cttcctcaac tgtcatatat
     1321 gcatggttac gaccaccacg tgtttcttga aggagagagt cgagtccgaa ctcctcatat
     1381 tttttacatt ttgctaaatc gttgtttgat tacagtctaa aagctctata atctctttat
     1441 aagatttgcc catcagacga aatataatag attgaaacta gaatagtaca cctctacttc
     1501 taaaacattg ttagaaatcg atttgtcctg ttcttgtttc attttactat agaacgattt
     1561 gaaggcgttt ataatattta gctgtacgag agtcttttaa aagtgttttg atggtttgga
     1621 tttcttcttt agttgatttc atattactat tgtataatgc tttttgattt tagtttagta
     1681 ttatcgtaga aaatggactc ggtgctatgg atacaccgga tgagaatggt tatgtagcag
     1741 atgactatcg gattacttac ttagaggccc acatcaaggc catgcgagat gccatttacc
     1801 aagatggggt tgacttgctt ggttatacga cttggagctg tatcgatccg gtttcagctg
     1861 gcacagggga aatgaataag cgctatggct ttatctatgt agatcgagat aatgtaggaa
     1921 atgggactct caaacgtagc aagaagaagt ccttctattg gtacatgtca tttatagcaa
     1981 tggtgtaagc atcgagtaga gcacatttgt ttactatttt tataaaatct tctccctgct
     2041 ttataagatg taaaaaagag ttcaaatgga actctttttt tgctataatg aggggagaaa
     2101 aatcagacag gagattaaga tgtcagaacc attattttta caatcagtta tgcaagaaaa
     2161 aatctggggt ggtaccaagt tacgtgatga gtttggctac gacatcccaa gtgaaaaagt
     2221 cggagagtac tgggctatct cagctcatct aaatggtgtt tctaaagttg ccaatggtcg
     2281 ttacgaggga acagatcttg ctactttgta tgcggaacac cgtgaattat ttggcaatcg
     2341 tccagaacct gtatttccgc ttttgaccaa gatcctcgat gccaacgact ggctcagtgt
     2401 ccaagttcac ccagacgatg cttatggact cgagcatgaa ggcgaacttg gaaaaacaga
     2461 atgttggtac atcattgcag cagatgaagg ttcagagatt atctacggtc acaatgccaa
     2521 gtcaaaagaa gaactccgcc agcaaatcga ggataagaac tgggatgcgc tgttgacaaa
     2581 agtgccagtt aaagctggag atttcttcta tgtaccaagc gggactatgc acgctatcgg
     2641 ggcgggtatc ttaatccttg aaacccagca gtctagcgat accacctatc gcgtctatga
     2701 ctttgaccgc aaggatgaca aaggcaactt gcgtgaactt catcttgaaa aatccatcga
     2761 tgtcttgaac attggtgagc cagcaaatag ccgtcctgta actgttaaag cagatgattt
     2821 gcgttccact ctccttgtat ctaatgattt cttcgcagtt tacaagtggg aaattactgg
     2881 aaaagttgac tttgaaaaga cagctgacta cagcttattg agtgtcttgg ctggtcaagg
     2941 tcagctaact gttgacggga aaaattatcc aattcaaaaa ggcagccact ttatcctacc
     3001 aagtgatgtt gaagcttgga ctctggaagg gcaaggtttg gaattgattg ttagccatcc
     3061 ataaaaagaa agggcctgag ttcactactc aagtcctttt tgattacata aattgggcaa
     3121 tgaagaccac aatgatgatg attggaatga cgaaacgaag aaacgaagaa acgaagaagg
     3181 aagagccaga cttggaacag tccttgtttc catgctcttt catcgagatg gagttcctcc
     3241 attgcaagag cctttttaaa gatatagcct gtaaaaagtg aaaggtagag agctccaaat
     3301 ggcatgagga gattggaaac caagaagtcc atagcgtcaa agaaggtctt accaaaaatg
     3361 tgaacatccg ccatgacacc gtaagatagg gctgaaggaa taccaaagac aaaggtcaaa
     3421 attcctaaaa taacactcca tttggcacgt ttgctgttat cctggttggt gatattgtct
     3481 acattgattt ccagcatcac gacagaaaaa gtgactgtcg caaaaaggaa gagcaagagg
     3541 aagaggacgt agaaaatggt tccaaaaggc atcttgtcaa agagttgagg caagacgata
     3601 aagagcaggc tgggtccccc ttcagactgg atattgaagg gggatcgagc ttggaaaatg
     3661 gctagacctg ccatgatgga tatcgagata ttcatggcta cgatggagat tcctgactgg
     3721 actagattgg ttttcttgtc taagtaagaa gcataggtca acatgactgt aacccctagt
     3781 gagagggcaa agaaagattg tcccagagca tagaggagac cagtgctagt cagttttgaa
     3841 aagtctggtt tgaggaagta aagaacccct tccatggcat ttggcaaact gagagagcga
     3901 ccgatgataa aaacaaagac gataaagagc aggggcatca tgactttcga agctctttca
     3961 atcccttttt gaaccccacg tgatacaatg aagatattca ataggataaa ggccgcttga
     4021 gctcctaggg caatggctgg atttgaaatg attgaagtaa ataactgagc ataatcaccc
     4081 gttccaccaa gttggaacaa tttcccaaac tcaataccta gatagactag aatccatcct
     4141 ccgataacac tgtaaaaaga taagaggata aagagggcaa aggcgccaat ccaaccgata
     4201 aagttgtact tgttattctt gcccagtttt ccaaaggttt tgatatcgga aacgccagca
     4261 ctacggccaa gggcaaactc agccagcagg agagggaaac cgattaaaat agtggaaatg
     4321 agaaagatta gtaaaaagcc tccaccgcca ttagcagcag tcatgtaggg aaacttccaa
     4381 acggaaccaa gcccgatggc cagccagcag atgctagaat aaaaccaagt ttcgaccccc
     4441 attgtgattt ttctgacata gttaaactct gatctccttt ttcaaaataa gaaaaagcta
     4501 cttgagttgt caaatagctt tctgtttgat tgatagactt gactatccta tcatgttttc
     4561 taaggtctgt caagaaaatc attttcaagt tttcacacct ttctcaaaaa agttaaaaaa
     4621 ttttctcaaa aacgcttgac tctgacctaa ggggaggggt tatactatca ttgtaaggag
     4681 gaaatcatgt accatataaa agaagctgcg cagctttcag gtgtctctgt caagaccctg
     4741 catcactatg acaagatagg actcttggtc cccttaaagt cggaaaacgg ctatcgaacc
     4801 tacagtcaag aggatttgga acgccttcag gtcattcttt actacaaata tctaggcttt
     4861 tctttagaga aaatagcaga gctgttaaag gaagaaagga cagatttatt gccccatttg
     4921 actaggcagt tggactatct aactcgcgaa aggcaacatc tggatacctt gatttccacc
     4981 ttgcaaaaaa ctattcaaga acaaaaagga gaaagaaaaa tgaccattga ggaaaaattc
     5041 acgggattta gctatcaaga caatcaaaaa taccaccaag aagcggtaga gaaatatggt
     5101 caagaagtca tgggacaagc gctcgaacgc caaaaaggtc acgaagacga ggctacggcc
     5161 gcctttaacc aagtctttca aactttggca caaaatcttc aagttggttt acctgcaaca
     5221 gcaaccgaaa accaggagca agcagccaag ctcttgcaag ccattcgcac ttatggattt
     5281 gactgctcta ttgaggtatt cggtcatatc ggtaaaggtt acgtctacaa cccagagttt
     5341 aaggaaaaca ttgacaagtt tggttctgaa acagcccagt acacgtcaga tgccattgcg
     5401 gtttacgttc agacaaatgc agaataaata ggctaggaat ttcctagcct attttttact
     5461 tcaaatcata aagccagtcg tcaccgtttt tgtagtaaaa gaattcactg agatcttctt
     5521 ctagaaacac acgaagcata tcagacatat catcggttgc aagttttaga tgagaaagat
     5581 tttcaaagtc ctcccaccaa actttccctt cgtctgaaga ctggagttca ccagtaaagt
     5641 gttctgtctt gtaaaaaagg acgacataac gataatcctt gtcgtcatac cagtttttga
     5701 taccacagag ttggggtttg gaaatgatca gaccagtttc ttctttcact tcacgaatga
     5761 cagcatcgac aaaggattcg ccacgttcaa catgaccacc aggaaaagta atgccagacc
     5821 agtcgggact aactcggtct tggaccagga ccttatctcc gtttttaatc atacacatgt
     5881 taacaaattc gactgcctct cttctgttca ttcttcacaa cctttaatct ttaatcataa
     5941 tgcagacttc ccgccaccca gccggtacag agggcagaag tgatgttaaa gccacccgtg
     6001 tgggcattga tatccataac ttcgcctgca aagtggaggc caggtaccag cttactttca
     6061 agggttttag gattgatttc cttgagactg actccaccct tggtaacaaa ggactttgca
     6121 agggacattt ttccagttac aggaatttta agttctttaa tggactggac aagttgttct
     6181 cgttcctttt cagtcagttg tttgactttt tcaggatatc cttgtacaaa aaattcggcc
     6241 aagcgttctg gtaacaaggt ttttaaagcg tttttcaagg atttttcccg attttcttct
     6301 agaaatgtaa ccaagtcctt ctcagaaagt tgaggcaaaa catcgagtga gagaacctcc
     6361 ccacctttga caaagctaga catgcgtagg gcagcaggac ctgacaaacc aaagtgggta
     6421 aagagtaaat catgagtgat gacatgctta ccataactta gggtcacatc gtccagagaa
     6481 ataccttgta aggctttatg tggaaaatct gttaataaag gactttcagc agcctcaaga
     6541 tcggtgatgg tatgcttaaa atggcgagca atctcgtgac caaaaccagt cgaaccagtc
     6601 gaaggataag acttaccacc tgttgtgaca atgagtttct cacaagtgaa ggtttgatcc
     6661 gctgacttaa ggacaaactg gtcatctact tttttaacag aaacgatttc tatttgagta
     6721 gcaacttgac cacctagttc ggtgattttc ttttccaaag cttcgataat agtccgagac
     6781 ttgtcactgg ctggaaagac gcgtccgtgg tcttcgacct taagtttaac accattttct
     6841 gtaaaaaagt tgatgatgtc atgattatcg aactgggaga aaacactgta aagaaagcgt
     6901 ccgtttccag gaattccagc tagcaggttg tctaagctac cattgttggt cacattgcaa
     6961 cgtcccccac cagtcccagc taattttttt ccaagtttcc gatttttttc gatgaggagg
     7021 gttttctgtc cataaaagct actggaaatc gtagccatca taccagcagg tcccccaccg
     7081 atgacaatag tatcaaaatg tttcatagct ctattgtacc acaaaaaaac aagagatgat
     7141 ggtcacctct tgtcaagaat gcaattaatc aatttcatag cccatcagca aaccgccctc
     7201 ttctgcatag aaactgcaga gaccagaggt tggtagaatt ttaatatccg cttgtgggaa
     7261 ggtttcacgg attcgctctg agagctgttg acaacatttt tcgttattgc gttgggccat
     7321 gacaatacgg ccaccagcat atccagcttt tactaattca tcataggcag cttgaactga
     7381 tttctttgat ccccttgctt tttgtagcaa ttcgagagtc ccagtttcac tagcttttcc
     7441 gaccatacga atgttgagaa ggccaacgac cgtaccgata agcttgctca aacggccgtt
     7501 cttcaccaag ttatcgactt tggctaggac aaagagcaac ttagtttttt cttgataggc
     7561 ggtgatagct tcaaccactt cttcaaaaga caagccctgg tcaatcaagt cattcaattt
     7621 ttctacgagt aggtcaactt caccaccagc agataaacta tcaatcacat gaatcttagt
     7681 gtcaggatgg tcttccagat aaatattctt tgctagttga gcactattgt gactgccaga
     7741 aagggtacct gtgatggtta ctaggaaaat gtttttggca ccttcaaatg ctcgcaaata
     7801 gtcatctggg cttggacaag ctgattttga agcttctgca gttgcataca tggtttccat
     7861 catttggtca atatcgagac tggcgtcatc aacaaagacc tgatcagcta cttgaatggt
     7921 taaggggaca cttacaaagg ttgtgttaat agctggtgtt ggcagttgac gataatcaca
     7981 accagagtca gcaataatct tccaagtcat agaaattctc catctttgtc agttatacat
     8041 tgacaaaggt tctgtctttt tttacaatta tatcatgaaa actcttgaaa caaaagcatc
     8101 atccgtctgt tgtgacaagt agaaagaaaa tgttatgtct gaacgtagaa tctctgaaaa
     8161 gtctcttgaa aatctcagaa aatcaaacca agaatccaat ttattaacca gagaagccat
     8221 tgaaacagcc ctcttgcaac tcttggaaaa aaaggagctg accaagatta gtatttctga
     8281 attggtcaaa cgtgcaggtg tttcgcgtgc ggccttttat cgcaattatg actccaaaga
     8341 ggaaatttta gagagcgtct ttaaacgaac tgtccacaat attatggaac agctgcatca
     8401 ttacgattta aagacagacc tttatttggt ttgggttcac cttttccggg agaccagaaa
     8461 ggaagctaga gtaattcaat tggccttgga ttaccatctg gaaaaaatct ttgtccaagc
     8521 catgcaggaa tttctagaaa aataccatgg gaaatcaaaa ggtgtcagct cttatcttca
     8581 ttccttctgg agctcagcca tcgtctctgt ccttctaaaa tggatcaagg atggcatgaa
     8641 ggtaccagct gaaaagattg cagatttagg tttaccattt tttaaaaaat agagaaaaag
     8701 gagaaaagag atgactgaaa aaagactggc ctgggatgag tattttgcag cccaagccct
     8761 actaattgcg aatcgttcca cttgtaaacg tgctaaagtg ggcgcgattc tggtaaaaga
     8821 taataaggtt atttccactg gttacaatgg ttcggtgtca gggactgagc attgtattga
     8881 tcacgaatgt ttggtcattg aaggccactg tgttcgcacc cttcacgctg aggtcaatgc
     8941 tatccttcaa ggtgctgagc gtggtgttcc caaaggcttt acagcctatg taacccattt
     9001 tccttgtctc aactgtacaa aacaattgct tcaggtcggc tgcaagcgcg tggtttatat
     9061 caaccagtac cgaatggacg actacgccca atacctttat caagaaaaag ggacagaatt
     9121 gacccattta ccacttgaga cagtacagac agctcttaaa gaggctgatc taatgtaaaa
     9181 attatcaaaa ataaatggtt tagaaagatt tttaaaccgt tttttggtat aataagaaga
     9241 ataaattgaa agaaggaatt ccaaaaatgg gaaaaattga agttattaat cacccactga
     9301 ttcaacacaa attgtcaatc ttgcgtcgta cagatacttc tacaaaagct tttcgtgagc
     9361 tagtagatga gattgcaatg ttgatggggt atgaagtact tcgtgatctt ccactagaag
     9421 atgtggaaat cgaaacacca attacaaaaa cagttcaaaa acaattggca ggtaagaaat
     9481 tggccatcgt cccaatcttg cgtgcaggta tcgggatggt tgatggtctc ttgagcttgg
     9541 ttccagctgc taaagttggc cacatcggta tgtaccgtga tgaagaaaca cttcaaccag
     9601 ttgaatactt ggtgaaattg cctgaggaca ttgaccaacg tcaaattttt gtagtagacc
     9661 caatgttggc aacaggtggc tcagcaatct tggctgttga ttctcttaaa aaacgtggcg
     9721 catcaaatat caaatttgtc tgccttgtat ctgctccaga gggtgtaaaa gcccttcaag
     9781 aagctcatcc agatgtagaa atctttacag cagccttgga tgaacgtttg aacgaacacg
     9841 gttatatcgt tccaggtctt ggagatgctg gagaccgctt gttcggtaca aaataagatc
     9901 gaaaagaaga atgacttgga aaagcatttc tagtcacgaa aggaggttgg gtttttgttt
     9961 ctgtctaatg aaagcagagc aaaaatttga ccttttttga ccaagatatt ataatagtct
    10021 tatcttcagt catttgacca acaaaattaa aactcaaaag gagaaatgaa tgattcctgt
    10081 agttattgaa caaacaagcc gtggagaacg ttcttacgat atttactcac gtcttctcaa
    10141 agaccgcatc attatgctga caggtccggt tgaagacaat atggctaact ctgttattgc
    10201 ccaattgctt ttcttggatg cccaagatag tacgaaagat atttaccttt atgtcaatac
    10261 accaggtggt tctgtttcag ctggtttggc aatcgtagat accatgaact ttatcaaggc
    10321 agatgtccaa accattgtta tgggaatggc tgcatctatg gggactgtca tcgcatcaag
    10381 tggggcaaaa ggcaaacgtt tcatgcttcc aaatgctgaa tacatgattc accaaccaat
    10441 gggcggtaca ggtggtggta cccaacaaac tgatatggct atcgctgcag aacacttgct
    10501 caaaactcgt aataccttgg aaaaaatctt ggctgaaaat tcaggtcagt caatggaaaa
    10561 agtccatgca gatgcagaac gtgataactg gatgagcgcc caggaaacac ttgaatatgg
    10621 ctttattgat gaaattatgg ccaacaattc attgaactaa tgatgataga aggcaaactc
    10681 gactgggttt gctttttttg gtataatagg gagagatttc ttagaaagag gatttatcat
    10741 gtttgaaaaa gtcaatcgct ctggcttgat tatctatctt tactataatc gtgatgccaa
    10801 aaaactgcag gattatggag atattaccta tcattccaag aaacatcgtt acttacaact
    10861 ctatgttcca actcaagaag tggagcaatt ggtcggacgc ttgagcaagg aaaaatttat
    10921 taaaaaagtt agggtttgtc atatccaaga gttggaaaca ccctttgtgg gcaatcttta
    10981 tcgataggaa aacgttatca tcgaaaaagt tcaaggaaaa cattgacaat tttctgataa
    11041 ttcggtatat tcttaacaga ctatttaaga aataaggaga caaaaagatg aagaaaaaat
    11101 ttgccctatc gtttgtggcg cttgcaagtg tagcacttct tgcagcctgt ggagaagtga
    11161 agtctggagc agtcaacact gctggtaact cagtagagga aaagacaatt aaaatcgggt
    11221 ttaactttga agaatcaggt tctttagctg catacggaac agctgaacaa aaaggtgccc
    11281 aattggctgt tgatgaaatc aatgccgcag gtggtatcga tggaaaacaa atcgaagtag
    11341 tcgataaaga taataagtct gaaacagctg aggctgcttc agttacaact aaccttgtaa
    11401 cccaatctaa agtatcagca gtcgtaggac ctgcgacatc tggtgcgact gcagctgcgg
    11461 tagcgaacgc tacaaaagca ggtgttccat tgatctcacc aagtgcgact caagatggat
    11521 tgactaaagg tcaagattac ctctttattg gaactttcca agatagcttc caaggaaaaa
    11581 ttatctcaaa ctatgtttct gaaaaattaa atgctaagaa agttgttctt tacactgaca
    11641 atgccagtga ctatgctaaa gggattgcca aatctttccg cgagtcatac aagggtgaaa
    11701 tcgttgcaga tgaaactttc gtagcaggtg acacagactt tcaagcagcc cttacaaaaa
    11761 tgaaagggaa agactttgat gctatcgttg ttcctggtta ctataatgag gctggtaaga
    11821 ttgtaaacca agcgcgtggc atgggaattg acaaaccaat cgttggtggt gatggattca
    11881 acggtgagga gtttgtacaa caagcaactg ctgaaaaagc atcaaacatc tactttatct
    11941 caggcttctc aactactgta gaagtttcag ctaaagctaa agccttcctt gacgcttacc
    12001 gtgctaagta caatgaagag ccttcaacat ttgcagcctt ggcttatgat tcagttcacc
    12061 ttgtagcaaa cgcagcaaaa ggtgctaaaa attcaggtga aatcaaggat aaccttgcta
    12121 aaacaaaaga ttttgaaggt gtaactggtc aaacaagctt cgatgcagac cacaacacag
    12181 tcaaaactgc ttacatgatg accatgaaca atggtaaagt tgaagcagca gaagttgtaa
    12241 aaccataata gaaaaatgtt gaaataggga atgagccttt gactcactcc ctgtttcgat
    12301 atttaatact cttcgaaaat ctcttcaaac tgcgtcaacg tcgccttgga ttatatatgt
    12361 gactgacttc gtcagtctta tctacaacct caaagcagtg ctttgagcaa cctgtggcta
    12421 gtttcctagt ttgctctttg attttcattg agtataagaa cctatcaaaa agtgagggaa
    12481 acccctcggg attataaata gaaagagtga atcttatgct ccaacaactc gtaaatggtt
    12541 tgattctagg tagtgtttac gcgctgttag ccctaggata taccatggtt tacggaatta
    12601 tcaagctcat caacttcgcc catggtgata tttatatgat gggagccttt atcggttatt
    12661 tcttgatcaa ttctttccaa atgaatttct ttgtagcgct tattgtagct atgctagcga
    12721 cagctattct tggtgtcgtg attgagtttc ttgcttaccg acctttgcgc cactctactc
    12781 gtattgctgt tttgattacg gctattgggg tttctttcct attggagtat ggaatggtct
    12841 atctggttgg tgccaatacc cgtgccttcc ctcaagcgat tcaaacagtt cgatatgatt
    12901 tgggaccaat tagcttaaca aatgtgcagt taatgatttt ggccatttcc ttgattttga
    12961 tgattttgtt acaagtcatt gtccaaaaga ctaagatggg gaaagccatg cgtgcagtat
    13021 cagtagatag cgacgcggcg caattgatgg ggatcaatgt aaaccgtacg attagcttta
    13081 ccttcgcttt gggttctgct cttgcgggtg cggctggtgt tctgattgct ctttattata
    13141 actctcttga gcctttgatg ggggttactc caggtcttaa atctttcgtt gccgcagtac
    13201 ttggtggtat cggaattatt cctggtgcgg ctcttggtgg ctttgtgatt ggtctattgg
    13261 aaacctttgc gacagccttt gggatgtcag atttccgtga tgccattgtt tatggaatct
    13321 tgttgttgat cttgattgtc cgcccagctg gtatccttgg taagaatgtg aaagagaagg
    13381 tgtaaacgat gaaggaaaat ttaaaagtta atattctatg gttactcctt ttgttagctg
    13441 gctatagctt gattagtgta ctggtttcag tcggagtact taatctattc tatgtacaga
    13501 ttttacaaca aattggaatt aatattattt tggctgttgg tctcaactta atcgttggtt
    13561 tttcaggaca attttcactt ggtcatgctg gtttcatggc gattggtgcc tatgcagcag
    13621 ctattattgg ttctaaatca ccaacctacg gtgccttctt tggagctatg cttgtaggga
    13681 ctttgctttc aggagcagtt gccttacttg tcggcattcc aaccttgcgc ttgaaggggg
    13741 actatcttgc ggtagcaact ctgggtgttt ctgaaattat ccgtatcttt atcatcaatg
    13801 gtggaagcct tacaaatggt gcggcaggta tcttagggat tcctaacttt acaacttggc
    13861 aaatggttta cttctttgtc gtgattacaa ccattgcaac cttgaacttc ttgcgtagcc
    13921 caattggtcg ctcaaccctc tctgttcgtg aagatgaaat cgctgctgag tcagttgggg
    13981 ttaatacgac taaaattaaa atcatcgctt ttgtctttgg tgccattact gcaagtattg
    14041 ctgggtcact tcaggcagga tttatcgggt ctgttgtacc gaaagattac accttcatca
    14101 actcaatcaa cgttttgatt attgttgtat ttggtggact cggttccatt acaggtgcga
    14161 ttgtttcggc tattgttctg ggaattttga atatgcttct ccaagatgtt gctagtgtgc
    14221 gtatgattat ttacgctttg gccttggtat tggtaatgat tttcagacca ggtggactcc
    14281 ttggaacatg ggaactgagc ctatcacgtt tctttaaaaa atctaagaag gaggaacaaa
    14341 actaatggca ttacttgaag taaaacagtt aaccaaacat tttggtggtc taacagctgt
    14401 tggagatgtg actcttgaat tgaacgaagg ggaactggtt ggattaatcg gtccaaacgg
    14461 agctgggaaa accacccttt tcaacctttt gaccggtgtt tatgaaccaa gcgagggaac
    14521 agtaacccta gatggtcacc ttttgaatgg gaaatcacct tataagattg cctctttggg
    14581 acttggacgt actttccaaa atatccgtct ctttaaagat ttaacagttt tagataatgt
    14641 tttgattgct tttggaaacc atcacaaaca gcatgttttt actagtttct tacgcttacc
    14701 agctttttac aagagtgaaa aagaattaaa ggctaaagct ttggaattgt tgaaaatctt
    14761 tgatttagat ggtgatgcag agactcttgc taaaaatctt tcctacggac aacaacgtcg
    14821 tttggaaatt gttcgtgccc ttgctacgga acctaaaatt ctcttcttag atgaaccagc
    14881 agcaggtatg aacccacagg aaacagccga attgactgag ttaattcgtc gtatcaaaga
    14941 tgagtttaag attacaatca tgttgattga acacgatatg aatctggtca tggaagtaac
    15001 agaacgtatc tacgtacttg aatatggccg tttaatcgct caaggaactc cagacgaaat
    15061 taagaccaat aaacgcgtta tcgaagctta tctaggaggt gaagcctaat gtctatgtta
    15121 aaagttgaaa atctttctgt gcattacggt atgatccaag cagttcgtga tgtaagcttt
    15181 gaagttaatg aaggagaagt tgtttccctt atcggtgcca acggtgcagg taagacaact
    15241 attcttcgca ccttgtcagg tttggttcga ccaagttcag gaaagattga atttttaggt
    15301 caagaaatcc aaaaaatgcc agctcagaaa atcgtggcaa gtggtctttc acaagttcca
    15361 gaaggacgcc acgtctttcc tggcttgact gttatggaaa atcttgaaat gggagctttc
    15421 ttaaagaaaa atcgtgaaga aaatcaagct aacttgaaga aggttttctc acgctttcct
    15481 cgtcttgaag aacggaagaa ccaagatgca gccactcttt caggggggga acaacaaatg
    15541 cttgccatgg gacgcgccct catgtcaaca ccaaaacttc ttcttttaga tgaaccatca
    15601 atgggacttg ccccaatctt tatccaagaa atttttgata tcattcaaga tattcagaag
    15661 caaggaacaa cggtcctctt gattgaacaa aatgccaata aagcacttgc aatctctgac
    15721 cgaggatatg tactggaaac agggaaaatc gtcctatcag gaacaggaaa agaactcgct
    15781 tcatcagaag aagtcagaaa agcatatcta ggtggctaaa acaatccagt ggattgtttt
    15841 agtcggcaga tggagattac gaagtaatca tcaatatagt ccgggggacc tttttagtcg
    15901 gtagattgag attgcaaaca aatctgcatc tacattgaaa gcttaatttc taataattga
    15961 aaaaatcgaa tgaaaaattt cttaccttca ttcacagagc tcgatttcag agctcttttt
    16021 gctagcttat tcatactttt ctgaatttcg aaaaagaaat gtaagcgttt gatagattta
    16081 caaaaagatt gtataatggg gataagaata gaaaaggaga agtctcatgg cagttaaaga
    16141 ttttatgacc cgcaaggtag tttatattag tccagataca acagtatctc atgcagcaga
    16201 tttgatgaga gagcaaggtt tgcaccgtct gcctgttatc gaaaatgatc aattagttgg
    16261 tttggtgact gagggaacca ttgcacaagc aagtccatct aaagcaacaa gtctttctat
    16321 ctatgagatg aattatcttc tgaataagac aaaagtaaaa gatgtcatga ttcgcgatgt
    16381 tgtcactgtc tcaggctatg ctagtctaga agatgcaact tatctgatgt tgaaaaataa
    16441 gattagtatt ctccctgtcg tagataacca tcaagtatac ggagttatta ctgaccgtga
    16501 cgttttccaa gcctttcttg aaattgcagg ttatggcgaa gaagggattc gtgtacgctt
    16561 tgttacagaa gatgaagttg gtgttcttgg aaaaattgtt tctttgattg tagaagaaaa
    16621 tttgaatatc tcccatacag tcaatattcc gcgtaaggat ggtaaggtga ttatcgaagt
    16681 gcaaatcgat ggatcaattg atttaccagc cttgaaagaa aaatttgaag caaatggtat
    16741 tcaagtggaa gaaatcgctc gcacttcagc aaaagtcttg taagaaggga agcccaaagg
    16801 cttctttttt catgaaaagg ggattagagc aaaagatgga aagaaatgat aaaatatgct
    16861 ataatgaaat gatgtaaaaa aggagtattt atggacattt cagtaattcg tcagaaaatt
    16921 gacgcaaatc gtgaaaaatt agcttctttc agggggtctc tttgacctcg aagggctaga
    16981 ggaagagatt gccatcttgg aaaacaagat gacagaacct gatttttgga acgataatat
    17041 tgcggcccaa aaaacgtcgc aagaattgaa tgaattaaaa aacacttaca ataccttcca
    17101 taagatggaa gagttgcagg atgaagtcga aattttattg gattttttgg ctgaagacga
    17161 gtcagtgcat gatgaactgg tagcgcagtt agccgaactt gataagataa tgaccagcta
    17221 cgagatgact ctactcttgt cagaacctta tgaccacaac aatgccatct tggaaatcca
    17281 tccaggttct ggtggtactg aggcgcagga ctggggtgat atgttgcttc gtatgtatac
    17341 tcgttatggt aatgctaaag gctttaaagt ggaagtgttg gattaccaag caggtgatga
    17401 ggctggtatt aagtcggtaa ctttatcatt tgaagggcct aatgcctatg gtctcctcaa
    17461 gtcagaaatg ggtgttcatc gcttagtgcg aatctcacca tttgactctg ccaaacgtcg
    17521 ccatacctct ttcacatctg tagaagtgat gccagaattg gatgatacta ttgaagtgga
    17581 aatccgtgaa gatgatatca agatggatac cttccgttca ggtggtgccg gtggacaaaa
    17641 cgtcaataag gtttcaacag gtgtacgttt aacccacatt ccaactggaa ttgttgtcca
    17701 atcaacagtg gatcgtaccc agtatggaaa tagagatcgc gccatgaaga tgttgcaggc
    17761 taagctctat caaatggagc aagagaagaa ggctgcggag gtagattctc tcaaaggtga
    17821 gaaaaaggag atcacttggg gaagtcaaat ccgttcttat gtcttcacgc cttatactat
    17881 ggtaaaagat caccgaacta gctatgaggt tgctcaggta gataaggtta tggatgggga
    17941 cctagatggt tttatcgatg cttatctcaa gtggcgaatt agctaagata gaaaggaact
    18001 cacatgtcaa ttattgaaat gagagatgtc gttaaaaaat acgacaacgg aacgactgct
    18061 ctacgcggtg tttcggttag cgttcaaccg ggggaatttg cttacatcgt aggaccttca
    18121 ggagcaggga agtcaacttt tattcgttct ctgtatcgtg aagtaaaaat cgataaagga
    18181 agtctatcag ttgctggttt taatctggtt aagatcaaaa agaaagatgt cccgcttcta
    18241 cgtcgtagtg ttggggttgt cttccaggat tataaactgt taccaaagaa aactgtctat
    18301 gaaaatattg cttacgctat ggaagtaatc ggggaaaatc gccgtaatat caaaagacga
    18361 gtgatggaag ttttggactt ggttggattg aagcataagg ttcgttcttt cccaaatgaa
    18421 ctctcaggtg gggagcaaca gcggattgcg attgcgcgtg caattgtaaa taatcccaaa
    18481 gtattgatag ctgatgagcc aacaggaaat ctggatccgg ataattcatg ggaaattatg
    18541 aatctcttgg aacggattaa cctacaagga acaactattt tgatggcgac tcataatagc
    18601 cagattgtaa ataccttgcg ccaccgtgtc attgccattg aaaatggccg tgtcgttcgt
    18661 gacgaatcaa aaggagagta tggatacgat gattagtaga ttttttcgcc atttatttga
    18721 agccttaaaa agtttgaaac gaaatggttg gatgacagta gctgctgtca gttcagtcat
    18781 gattactttg accttggtgg caatatttgc atctgttatt ttcaatacag cgaaactagc
    18841 tacagatatt gaaaataatg tccgtgtagt agtttatatc cgaaaggatg tggaagataa
    18901 tagtcagaca attgaaaaag aaggtcaaac tgttacaaat aatgactacc acaaggtata
    18961 tgattctttg aagaacatgt ctacggttaa aagtgttacc ttttcaagta aagaagaaca
    19021 atatgaaaaa ttaaccgaga taatgggaga taactggaaa atctttgaag gagatgccaa
    19081 tcctctctat gatgcctata ttgtagaggc aaacactcca aatgatgtaa aaactatagc
    19141 cgaagatgct aaaaaaattg aaggtgtctc tgaggttcaa gatggcggtg ccaatacaga
    19201 aagactcttc aagttagctt catttatccg tgtttgggga ctagggattg ctgctttgtt
    19261 aatttttatc gcagttttct tgatttcaaa taccattcgt attaccatta tttcccgcag
    19321 tcgcgaaatt caaatcatgc gcttggtcgg agctaaaaac agttatatcc gtggaccgtt
    19381 cttgttagaa ggagccttta tcggtttatt gggagctatc gcaccatctg ttttggtctt
    19441 tattgtttat caaattgttt accaatctgt caacaaatcg ttggtagggc aaaatctatc
    19501 catgattagt ccagatttat ttagtccgtt gatgattgcc ctactatttg tgattggggt
    19561 tttcattggt tcattgggat caggaatatc catgcgccga ttcttgaaga tttaggtaaa
    19621 atagctgctt ttatgaggag attgtaaaat ctcctttttt gctacaagag tttttgaaaa
    19681 gagatgcgca gaagaaaaga gcttccaaag aagtccccca gagaagactt cttgatggct
    19741 aatttgaact tgaaatttgg cgcactagaa attttttaga aaaaagtgta atttttttat
    19801 aaaagccttt acaaaaaaaa ttaaagtggt ataattaaat catagaaagt gcaaacgttt
    19861 tcgtaaaacg tttgcctaaa taaaaataaa ggagatttga atgatgaaag atacattcaa
    19921 aaatgtcttg tctttcgaat tttggcaaaa attcggtaag gctttgatgg tagttatcgc
    19981 ggttatgccg gctgctggtt tgatgatttc aatcggtaag tctatcgtga tgattaaccc
    20041 aacctttaca ccacttgtca tcacaggtgg aattcttgag caaatcggtt ggggggttat
    20101 cggtaacctt cacattttgt ttgccctagc cattggagga agctgggcta aagaacgtgc
    20161 tggtggtgct ttcgccgctg gtcttgcctt cattttgatt aaccgtatca ctggtacaat
    20221 ttttggtgta tcaggcgata tgttgaaaaa tccagatgct atggtaacta ctttctttgg
    20281 tggttcaatc aaagttgctg attactttat cagtgttctt gaagctccag ccttgaacat
    20341 gggggtattc gtagggatta tctcaggttt tgtaggggca actgcttaca acaaatacta
    20401 caacttccgt aaacttcctg atgcactttc attcttcaac gggaaacgtt tcgtaccatt
    20461 tgtagttatt cttcgttcag caatcgctgc aattctactt gctgctttct ggccagtagt
    20521 tcaaacaggt atcaataact tcggtatctg gattgccaac tcacaagaaa ctgctccaat
    20581 tcttgcacca ttcttgtatg gtactttgga acgtttgctc ttgccatttg gtcttcacca
    20641 catgttgact atcccaatga actacacagc tcttggtggt acttatgaca ttttaactgg
    20701 tgcagctaaa ggtactcaag tattcggtca agacccacta tggcttgcat gggtaacaga
    20761 ccttgtaaac cttaaaggta ctgatgctag tcaatatcaa cacttgttag atacagtaca
    20821 tccagctcgt ttcaaagttg gacaaatgat cggttcattc ggtatcttga tgggtgtgat
    20881 tgttgctatc taccgtaatg ttgatgctga caagaaacat aaatacaaag gtatgatgat
    20941 tgcaacagct cttgcaacat tcttgacagg ggttactgaa ccaatcgaat acatgttcat
    21001 gttcatcgca acacctatgt atcttgttta ctcacttgtt caaggtgctg ccttcgctat
    21061 ggctgacgtc gtaaacctac gtatgcactc attcggttca atcgagttct tgactcgtac
    21121 acctattgca atcagtgctg gtattggtat ggatatcgtt aacttcgttt gggtaactgt
    21181 tctctttgct gtaatcatgt actttatcgc aaacttcatg attcaaaaat tcaactacgc
    21241 aactccaggg cgcaacggaa actacgaaac tgctgaaggt tcagaagaaa ccagcagcga
    21301 agtgaaagtt gcagcaggct ctcaagctgt aaacattatc aaccttcttg gtggacgtgt
    21361 aaacatcgtt gatgttgatg catgtatgac tcgtcttcgt gtaactgtta aagatgcaga
    21421 taaagtagga aatgcagagc aatggaaagc agaaggagct atgggtcttg tgatgaaagg
    21481 acaaggggtt caagctatct acggtccaaa agctgacatt ttgaaatctg atatccaaga
    21541 tatccttgat tcaggtgaaa tcattcctga aactcttcca agccaaatga ctgaagcaca
    21601 acaaaacact gttcacttca aagatcttac tgaggaagtt tactcagtag cagacggtca
    21661 agttgttggt ttggaacaag taaaggatcc agtatttgct caaaaaatga tgggtgatgg
    21721 atttgcagta gaacctgcaa atggaaacat tgtatctcca gtttcaggta ctgtgtcaag
    21781 catcttccca acaaaacatg cttttggtat tgtgacggaa gcaggtcttg aagtattggt
    21841 tcacattggt ttggacacag taagtcttga aggtaaacca tttacagttc atgttgctga
    21901 aggacaaaaa gttgcagcag gagatctcct tgtcacagct gacttggatg ctatccgtgc
    21961 agcaggacgt gaaacttcaa cagtagttgt cttcacaaat ggtgatgcaa ttaaatcagt
    22021 taaattagaa aaaacaggtt ctcttgcagc taaaacagca gttgctaaag tagaattgta
    22081 atatacttga ggttggaagc tgtattccaa cctcttattt tgggagaaaa gaatgaaatt
    22141 tttaacactc aatactcaca gttggatgga gaaagaagca gaggaaaaat tccagatttt
    22201 gcttgaagat attcttgaaa aggactatga tttgatttgt ttccaagaaa tcaatcagga
    22261 gatgacctcg tcagaggtgg aggttaatga cctttatcaa gctttgccag cagctgagcc
    22321 tattcaccaa gaccattatg ttagactctt ggttgaaaag ttgttagagc aagggaaaaa
    22381 ttactactgg acctgggctt ataaccatat cggctataac cgctaccacg aaggtgtggc
    22441 tatcttgtct aaaacaccta ttgaagccag agaaattttg gtttcagatg tggatgatcc
    22501 aacagactat catactcgcc gtgttgccct agctgaaact gtagtcgatg gcaaggagct
    22561 agcagttgcc agtgttcatc tctcttggtg ggataaaggt ttccaagaag aatgggcacg
    22621 atttgaggct gtcttgaaaa aattgaacaa gccactttta ctagctggag attttaacaa
    22681 cccagcaggt caggaaggtt accaagctat tttagctagt ccattaggct tacaagacgc
    22741 atttgaagtt gctcaagaga aaagtggtag ctatactgtt ccgcctgaaa ttgatggctg
    22801 gaaagggaac actgaacccc ttcgaatcga ttatgtcttt actaccaaag agttagcggt
    22861 ggaaaattta catgtcgtat ttgatggtaa caagagtcca caagtgagtg atcactatgg
    22921 cttgaatgct atgttaaact ggaaataata actgaaaaga ggttggaact ataaaattcc
    22981 agccttttct tactagagaa gctactggaa atagcctaaa taagtgagac tactgtaatg
    23041 gaataaaata tggtataatt gataagatag atagaatcga ggatgttatg tcatttacga
    23101 aatttcaatt taaaaactat attagagaag ccttggagga gttaaaattt acaactccaa
    23161 cagaggtgca agacaagttg attcctattg ttttggcagg tcgtgaccta gtaggagaat
    23221 caaaaacagg ttcaggtaag actcatactt tcttgttacc gattttccag caattagatg
    23281 aagctagcga tagtgtacaa gcagtgatta ctgcaccgag tcgtgagttg gctactcaaa
    23341 tttaccaagt agcgcgtcag atttcagctc actcagatgt cgaagttcgt gtggttaatt
    23401 atgtgggtgg tacggataag gctcgccaga ttgagaaatt ggaaagcaat cagcctcata
    23461 ttgttattgg aacaccaggc cgtatctacg acttggttaa atctggtgat ttagctattc
    23521 ataaagccaa gacatttgtt gttgatgaag cagatatgac cttggatatg ggattcttgg
    23581 aaattgttga taagattgct ggcagtcttc caaaagactt gcaattcatg gtcttctcag
    23641 cgactatccc acaaaaactg caaccattct tgaaaaaata cttatcaaat cctgttatgg
    23701 agaaaattaa gactaaaacg gttatttctg acaccattga taattggttg atttcgacca
    23761 agggacatga taggaatgct caaatttacc aattgactca gttgatgcag ccgtatttgg
    23821 caatgatttt tgttaacact aaaacgcgtg ctgatgaatt gcattcatat ctgactgctc
    23881 aaggcttgaa ggttgcaaaa atccatggag atattgcccc tcgtgaacgc aagcgaatca
    23941 tgaatcaggt gcaaaatctg gattttgagt atattgtcgc aacagattta gcagcgcgtg
    24001 ggattgacat tgaaggtgtc agccatgtca tcaatgatgc cattccgcaa gacttatctt
    24061 tttttgttca ccgtgttggt cgtactggac gaaatggcct accaggtaca gctattaccc
    24121 tttatcagcc aagtgatgac tcggatatcc gtgagttgga gaaattggga atcaagttta
    24181 gtcctaagat ggtcaaagat ggggaatttc aagataccta tgaccgtgat cgtcgtgcca
    24241 accgtgagaa aaaacaagat aaacttgata tcgaaatgat tggtttggtt aaaaagaaaa
    24301 agaaaaaagt caaaccgggt tataagaaga aaattcaatg ggcggttgat gaaaagcgcc
    24361 gtaaaactaa gcgtgctgaa aatcgcgctc gcggtcgtgc agagcgtaaa gctaaacgcc
    24421 aaacatttta atagaaattg ttggagtact gagctccaac ttttttattt atgagaacga
    24481 actatctaaa ccgaaacact acattaaaga ctgcaaattg cgattaaaaa tggtataatg
    24541 ataaagttat atagtcccga taagatggta ggtatttatt acgaagagtt ttcctatcag
    24601 tagtttgcaa ttcgcaaaga atgacggctt cgaggttgct cgtctttcta cggttgccgc
    24661 catactgcgg tcaccctatg cgccttacta gcttcagaaa taaaaaagta tcgtttttat
    24721 ttctgaagct agtaactctt agataatgga aattcctatc agtactttgt aactctataa
    24781 caatattttt taagggggga catttttatg tcagaacgta aattattcac gtctgaatct
    24841 gtatctgagg ggcatccgga taagattgca gaccaaattt cagatgcgat tttggatgct
    24901 attttagcaa aggatccaga ggcgcacgtt gctgctgaaa cagctgtata tactggttct
    24961 gtccacgttt ttggtgaaat ttctacaaat gcctatgtgg atattaaccg tgtggttcgt
    25021 gataccattg cagagattgg ttataccaat acagaatatg gattttctgc tgagacggtg
    25081 ggagtacacc catctttggt ggaacaatct cctgacatcg ctcaaggtgt taacgaagcc
    25141 ttggaggttc gtggaaatgc tgatcaagat ccactggact tgattggagc aggtgaccaa
    25201 gggctcatgt ttggatttgc agtagatgaa acagaagagc tcatgccatt gccaattgca
    25261 ctcagtcata aattggttcg tcgtctggca gaacttcgta agtctggaga aattagctat
    25321 ctccgtccag atgcaaaatc acaagttaca gttgagtacg atgaaaatga ccgtccggta
    25381 cgtgtagata cagtcgttat ttctactcag catgatccag aggccactaa tgaacaaatc
    25441 catcaagatg tgattaacaa ggtcatcaaa gaagttattc catcttctta tcttgatgat
    25501 aagacaaaat tctttatcaa tccgacaggt cgttttgtaa tcggtggtcc tcaaggggac
    25561 tcaggtttga ctggtcgtaa gattattgta gatacttatg gtggctactc tcgtcatggt
    25621 ggtggtgcct tctctggtaa agatgcgact aaggtggatc gttcagcctc ttatgcggct
    25681 cgctatattg ccaagaatat cgttgcagca ggccttgcta agaaggcaga agtgcagttg
    25741 gcctatgcta tcggtgttgc gcaacctgtt tctgttcgta tcgatacttt cggtacagga
    25801 acagtagctg aaagtcaact tgaaaaagcg gctcgtcaaa tctttgacct tcgccctgca
    25861 gggattatcc aaatgctgga cctcaagcgt ccaatttacc gtcaaacatc ggcttacggt
    25921 cacatgggac gtacagatat tgatcttcca tgggaacgtt tggataaggt agatgctttg
    25981 aaagaagcag taaaataaga ttttaagagg ggaacgtcct ctctttttta tagtttttaa
    26041 ctatactggg atactgttct gaaaatccat tttgcgaaag tagagattta catgtatagt
    26101 agattgaaac tagaatagta cacctcaact tctaaaacat tgttagaaat cgatttgact
    26161 gtcctgatcg atttctcctg ttcttgtttc attttactat atttctttaa aaatgataaa
    26221 ggttgagatt tctcctcgta atagataatc atggggatat ttcaatccaa agttttattc
    26281 gttatcactt gactattgca aggttttcta gagcaacaga gtcatggaat ggactcatgg
    26341 ttgagatttc tccttgttgc ttggacttca ttcaaaagtc tgttacccaa gccttgttca
    26401 aacttccaat acactagctg tttccatagc atgacttctg tactagactt tcttttccga
    26461 ataaatagat agaaccacag aatctagtaa acctagaatt aaaattatgg tataatatta
    26521 gcaataaaag aaatctggag gattagaatc atggtatcaa cgaaaacaca aattgctggt
    26581 tttgagtttg acaattgctt gatgaatgca gcaggtgtgg cttgtatgac gatagaggag
    26641 ttagaagagg tcaaaaactc agcggcagga acctttgtta ctaagacagc gaccttggac
    26701 ttccgtcagg ggaatcctga gccacgctac caagatgttc cacttggttc catcaactct
    26761 atgggcttgc caaataatgg cttagactat tatttggatt atcttttaga tttgcaggaa
    26821 aaagagtcga accgaacttt cttcttatct ctggtcggca tgtctccaga ggaaacccat
    26881 actattttga aaaaagtcca agagagtgat tttcgtggtc tgactgagct aaatctttcc
    26941 tgtccaaatg ttccaggtaa acctcagatt gcctatgatt ttgagacaac agaccggatt
    27001 ttggcagaag tgtttgctta cttcaccaaa cctcttggaa ttaaattgcc accttatttt
    27061 gatattgttc actttgacca agcggcagct attttcaaca aatatccgct caagtttgtc
    27121 aactgcgtta actctatcgg aaacggcctc tatatagaag acgaatctgt cgttattcgg
    27181 cctaagaatg gttttggtgg aattggtgga gaatacatca aaccgactgc tctagccaat
    27241 gttcacgcct tttatcaacg tttaaatcct caaatccaaa ttatcggaac aggtggcgtt
    27301 ctgactggtc gagatgcctt tgaacacatc ctctgtggag caagtatggt gcaggtggga
    27361 acgacccttc acaaagaagg cgtcagtgct tttgaccgca ttaccaatga actgaaagca
    27421 atcatggtgg aaaaaggcta cgagagctta gaagatttcc gtgggaaatt gcgctatatt
    27481 gactaaatta aatcgaaaaa tcagaagaaa gaagggacga tgctagccat tgaagaaagt
    27541 cagaagttga ctttatcaaa tttaccgagc ctgagcctat ttacagggac agatcagggt
    27601 cagtttgaag tgatgaagag tcaaatgttg aaacagattg ggtatgattc tgctgacctc
    27661 aactttgcct actttgatat gaaagaagta gtttacaagg atgtggaact ggagttggtc
    27721 agccttcctt tctttgcgga tgagaaaatc gtgatattag attattttat ggatatcacg
    27781 actgctaaga aacgcttttt gacagatgat gagcttaagt catttgagga ataccttgac
    27841 aatccttctc caacaaccaa gttgataatc tttgcagaag gaaagctgga tagcaaaaga
    27901 cggttagtca aattacttaa gcgtgatgcc aaggtcttcg atgcagtaga agtaaaagaa
    27961 caagaattgc gccagtattt ccaaaagtgg agtcagaaac aaggtctgca gtttaccaat
    28021 cattcttttg aaaatctcct catcaagtcg gggtttcaat ttagcgaaat ccagaaaaat
    28081 cttctctttt tacagtccta taaggcgaat tctgttatcg aggaagagga tattgttaac
    28141 gcaattccca agaccttgca ggacaatatt tttgatttaa ctcagtttat tctgactaaa
    28201 aagatggatc aggcgcgcga tttagtgaga gacttgacct tgcaagggga agatgaaatc
    28261 aaactgattg cagtcatgct gggacaattt cggactttta ctcaggtgaa gattttggcg
    28321 gagtctggcc aaacagaatc gcagattgca agtagtttag gtagttatct gggacgtaac
    28381 ccaaatcctt atcaaatcaa gtttgcatta agagattcga gaggactttc tttgagcttt
    28441 ctgaagcaag ctatttccta tttgattgag acagactatc agattaagac aggtctttat
    28501 gaaaaaggtt tcctttttga aaaggcactc ttacagattg ctagtcaggt caattgacat
    28561 ttgttgaaac tacaacccgc gtcaggatta tcttgtcgtg ggtttcttgc tgatttgctt
    28621 attttattag aaagtatatc agtgatttcc ttatctataa gcaaaaaact tgaaaaaagt
    28681 gaatgtttgc gttatcattt tcatatagaa agaaaaagag gtattacaga tggctattat
    28741 cttaccagaa cttccatatg catacgacgc tttggaacca tacatcgatg cggaaacaat
    28801 gcatttgcac catgacaaac accatcaaac ttatgtcaac aatgccaatg cagctttaga
    28861 aaaacaccct gaaatcggtg aagaccttga agccttgctt gctgatgtag aatctatccc
    28921 agctgatatc cgtcaagcac ttatcaacaa tggtggcgga cacttgaacc acgctctttt
    28981 ctgggaattg atgactcccg agaaaacagc tccttcagca gaactggcag cagcaatcga
    29041 tgcaacattt ggttcatttg aagaattcca agcagccttc actgcagcag caacaactcg
    29101 ttttggttca ggttgggcat ggttggttgt caacaaagaa gggaaacttg aagtgacttc
    29161 aacagcaaac caagacacac caatctcaga aggtaaaaaa ccaatcttgg gcttggacgt
    29221 ttgggaacat gcttactacg tgaaataccg caacgtgcgt cctgactaca tcaaagcttt
    29281 cttttcagta atcaactgga ataaagtaga tgaattgtac gcagctgcta aataatgata
    29341 gttggaggga agaattgttc ttctcttttt taggttataa gattctggtc tgacaaaatc
    29401 gtcagacttt tttcattttt atgagaaacg tgctaactgc tagaaattat ggtagaataa
    29461 agtattaagt aatcgtggaa aaaggatatc tatgcgaaaa gaaattgcac ctgaattata
    29521 caattataac aagtttcctg gtccggagtt ccatttacac ggggacaagg tcgaaacgga
    29581 agggatagct ttttccttgg ttgaaaatat caaggatgcc tttgatgtga cgacttttaa
    29641 tcagcgtttt tcagaagtct taaccaagtt tgattatatc gtgggggact ggagcaacga
    29701 acagcttcgc ctacgaggtt tttacaagga tgaccgaaca gaagaaaaac ttgaaaaaat
    29761 cagtcgttta caagactacc ttttagagta ttgtagttat ggttgtgcct attttgtctt
    29821 agaaaatgaa gcccctaagc gagcatcatt tgacaagaaa atgcgtaaga cggaagaaga
    29881 aacaccttct agaaaaggaa agaaaccgac tcaaactaaa cgaaagtcga atgcagataa
    29941 gaaaaataga cgtcgtcaga aagaccagca ttctcagaaa gaggacaagg gacaacgtca
    30001 ttttgtcatt cgtcagaagt gagtagagaa taaggagaag aaatgaaacc ctcaattcat
    30061 agtttggtac atcaaaccat gcaggaatgg gtattggagc agggagaaaa gaaattccgt
    30121 gcagatcaaa tctgggaatg gctctaccgt aaacgtgtgc agtcatttga agaaatgacc
    30181 aacctttcca aggatttgat tgctaagctc aatgaccagt ttgtggtcaa tcccttgaaa
    30241 caacgtattg tgcaagagtc tgctgatggt acagtcaaat atctctttga attgcccgac
    30301 ggtatgttaa ttgagactgt actcatgcgt caacactatg gtttatctgt ctgtgtgacc
    30361 actcaggtcg gctgtaatat cggttgtacc ttctgtgcct ctggtttgat taagaagcag
    30421 cgtgacctca ataacggtga aatcgtagcg caaatcatgc tggttcagaa atactttgat
    30481 gagcgtggtc aggatgaacg catcagccat atcgttgtca tgggaatcgg tgagcccttt
    30541 gataattaca acaatgtctt gaatttcgtt tgtactatca atgatgataa aggaatggct
    30601 atcggtgctc gtcacatcac gatttcaacc tcaggtttgg ctcataaaat tcgtgacttt
    30661 gctgatgaag gggttcaggt caatcttgcc gtgtctcttc acgcacccaa caatgaactt
    30721 cgttcaagta tcatgaaaat taaccgtgcc tttccgattg aaaaactctt tgctgctatt
    30781 gaatactata ttgagacaac aaaccgtcgt gtaacctttg aatatatcat gcttaatgaa
    30841 gtcaacgacg gtgtagaaca agccttggaa ttggctgaat tgctcaagaa catcaagaaa
    30901 ttgtcttatg taaacttgat tccttataac ccagttagtg agcatgacca atatagccgt
    30961 agtcccaaag agcgcgtgtt ggctttctat gatacgctta agaaaaaagg ggtcaactgt
    31021 gtggttcgtc aagagcatgg tacagatatt gacgcagctt gtggacaatt gcgctctaac
    31081 acaatgaaac gtgaccgcca gaaggcagtc gcagcggtaa atccttaatg atgaagaaga
    31141 cttataatca tattttggtc tggggagtca ttttctatag catttgcatt gtctgttttt
    31201 gctttactcc tcaagaacaa tctaccgtgg gagtgggaac tccaggtatt cagcatcttg
    31261 gacgcctggt ttttcttttg actcctttca attctctctg gaaactgggc gaagtgagtg
    31321 acattggaca attatgttgg atttttttac aaaatatcct caatgtcttc ttgttttttc
    31381 ctctgatttt ccaactcctt tatctatttc caaatttgcg gaaaacaaaa aaggtccttc
    31441 tttttagttt tcttgtgagt cttggaatcg agtgtacgca attaatcttg gactttttct
    31501 ttgatttcaa tcgcgtcttt gagattgatg atttgtggac caacactttg ggtggctatc
    31561 tggcttggct cctttataaa cgattacata aaaacaaggt aaggaattaa aatgagtatt
    31621 ttagaagtta aaaatctgag tcacggtttt ggtgaccgtg caatttttga agatgtgtcc
    31681 ttccgtctcc tcaagggaga acatatcggc ctggtcggtg ccaatggtga aggaaaatca
    31741 acctttatga gtatcgtgac tggtaaaatg ctgccagatg aaggaaaggt tgagtggtcc
    31801 aaatatgtga cggctggtta cttggatcag cactctgtcc ttgctgaaag acagtcggtg
    31861 cgtgatgttc tccgtacggc ttttgatgag cttttcaaag ctgaagctcg tatcaatgac
    31921 ctctatatga aaatggctga agacggcgcg gatgttgatg ctctcatgga agaagtagga
    31981 gaacttcaag accgtctgga gagtcgtgat ttctatacct tggatgctaa gattgacgaa
    32041 gtagcgcgtg ctcttggtgt tatggacttt gggatggata cggatgtaac ttctttgtca
    32101 ggtgggcaaa gaaccaaggt gcttttggca aaacttctcc ttgaaaagcc tgatattttg
    32161 ctgttggacg agccgaccaa ctacttggat gctgagcata ttgattggct caagcgctat
    32221 ctccaaaact atgagaatgc ctttgttctt atttcgcacg atattccatt cctcaatgac
    32281 gttattaata ttgtctatca tgtggaaaat caacagctga cgcgttactc tggtgactac
    32341 taccagttcc aagaagttta tgctatgaag aaatctcagc tagaggcagc ctacgaacgc
    32401 cagcagaaag agattgcaga cctcaaggac tttgtggctc gtaataaagc ccgtgttgca
    32461 actcgtaata tggctatgtc tcgtcaaaag aaattggata agatggatat tatcgaactc
    32521 caaagtgaga aaccaaaacc atcctttgat ttcaaaccag ctcgtacacc agggcgcttt
    32581 atcttccaag ccaagaactt gcaaattggt tacgaccgtc ctcttactaa gcctttaaat
    32641 cttaccttcg aacgcaatca aaaggttgcg attattggtg ctaatggtat tggaaaaaca
    32701 actctcttga agagtctctt aggcattatc tcgccaatcg ctggggaagt ggagcgtgga
    32761 gattatttag aacttggtta ttttgagcag gaagtagaag gcggtaatcg ccaaactcct
    32821 cttgaagctg tctggaatgc ctttcctgcc cttaatcaag cagaagtccg tgcagccctt
    32881 gcccgttgtg gtttgacaac caaacatatt gaaagccaga ttcaagtatt atcaggggga
    32941 gagcaagcca aggttcgttt ctgtctcttg atgaatcgtg aaaacaacgt tttagtgctg
    33001 gacgagccga ccaaccattt ggatgtggat gcaaaggatg agctcaaacg cgctctcaaa
    33061 gaatacaggg gatctatcct tatggtctgc cacgagccag acttttatga aggttggata
    33121 gaccaaatat gggattttaa taatttaact taagaatgag agtaaaagaa atacagtacc
    33181 gaatgtggat actgtatttt tttggttttt aagatttaaa atcgtagtct ttcaccactt
    33241 cgatgctgtc gattgtgata gcagtagttg gcttgtcttt ttcatctttt tcggctttag
    33301 caatcttatc cacaacatcc ataccgtcaa tcacttgacc aaagactggg tgtttgccat
    33361 ctagactagg gtttccacct tctttgtagg cttcgataat tttctgtgga tacttgcttg
    33421 tagggagttt agaagaggta tctgtagagt tttggttgat gaagaactgg ctgccattgg
    33481 tgtttggttg accagtatta gccatagcaa gagcaccacg gatgttatac aaataaggag
    33541 taatctcgtt cttgaaacca gttcctttgt ctttagtctt atccttgtca tgccatatgg
    33601 actgaccacc tgtaccgtcc ccttttggat ctccagtttg gaccataaag ccatcgatga
    33661 cacggtggaa ggtaataccg ttatagtagc cttctttggc gtgagtgagg aaattttcaa
    33721 ccgctagagg agcgagttta gggaagagtt taatgcgaat atcaccttgg cttgtgtgga
    33781 gaatcacttc ggcttcatct tcagcaactt ccttagatag ttgtgggaaa ttggcgtttt
    33841 cgtttgttaa agcatcgttt aactccttgg cagattgggc agctactttg gaactttctt
    33901 cagcagcaag actggaatca acataatcat caccacgcag actgcgttgg acgctgctac
    33961 acccagctag ggctacagta gacagtaaaa gaagggttgc tagttttttc attctgatcc
    34021 tctcaaaaat tcatttatac cattgtaccc taaaaggaag tggatttcaa atattagtag
    34081 tttacagaac tggcttagat aatagttggg atttcatcat catcttgaaa atcttcaact
    34141 tcttcaactt cttccccagt aaatggtttc tgaaaagaac ttagtataaa acgggcaatt
    34201 ggttcaacaa ttaggaaatt tagaggaaga gctgcaataa agtttacgaa ccaggcgctt
    34261 agataggtat tgggtgtgat agcaatgcca ttgtaaaaaa gtccatagaa ggacatgaaa
    34321 gtgaccagct aattcttctt ctgaaaaatg ctcatgaagg gcttgatcca ggtcgcaaga
    34381 tttcatttta gaaagtccag taactccctt cttcttcagg atattctttt tcatggtatt
    34441 atttagatgg tcaagtgagt caaaagaagt ttcgacaaga gcataccctt tttctaccag
    34501 agtttttaga tgtttagggg catcaatacc ataagtgtac tcaaagtatt ttggaaacca
    34561 gatcttaaga aagctcgtaa agctagccag ttctctaaac gctaagaact agctacaaca
    34621 cagcatttac aacacttcat ggttcacacc atggggtgtt ttttaatgat tttaagcaaa
    34681 attcacactt aattgaaagt tatttaaatt agatgaaaat ttattttttg aaaaagtggg
    34741 aaaacattga ttctaagcga attttgaatc atattcgaat gggagttttt aaaatcatgt
    34801 ttcagtggta aaagtaccaa actggattct ccatagtaaa atgatatctc ctgctagtga
    34861 atcatctgct aataagtcca tatttcgctt gggacttaga agccaagtat ccaactctcg
    34921 ttcaggcgat atgaagggtt tgacttggtg ttctttataa aatttttcta aaatagtatt
    34981 cacggatagc atctttcaaa catttatttc ctattctaac aaatttctaa atgctttaag
    35041 aaatttatag tctgtcaagt ttttttcttg acacctgcca gaaatctgct ataatagaac
    35101 atgtgctaaa tagctcagct atttcaccga aataaaaaat taaagaaatg agactttaaa
    35161 aatggcagtt aaaatccgtt tgactcgtat gggttctaag aaaaaacctt tctaccgtat
    35221 caacgtagca gattcacgtt caccacgtga cggacgtttc atcgaaacag ttggaactta
    35281 caacccactt gttgctgaaa accaagtaac tttgaaagaa gaccgcgttc ttgcatggtt
    35341 ggctaatgga gctcaacctt cagacacagt acgtaacatc ctttcaaaag aaggcgtatt
    35401 gaaaaaattc cacgattcta aattctcaaa ataagtttaa agtaggttga cagatggata
    35461 cgattgaaaa tctcattatt gcgattgtga aacccttaat ttcacaacca gatgccttaa
    35521 ctatcaagat tgaggataca ccagaatttt tggaatatca tttgaatctt gatcaaagcg
    35581 atgtgggtcg tgtaatcggt cgtaagggtc gcactatttc tgcgataaga acgattgtct
    35641 actctgtccc aactgaatac aaaaaagtaa gaatcgttat tgacgaaaaa taagaagggc
    35701 gggacggatg tctcgctttt ttgtgtggaa tagaagatat agtaaaatga aataataaca
    35761 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag taaaggtgta
    35821 ctattctagt ttcaatatac tataaaagat tgaacgttta ggcaattcca agactactta
    35881 atcgaacatt aacagcagtc tcggactgat gaaggtcttt ttatgaagtt agtggaggaa
    35941 gtcggagaag tagccgaggt tttgaatagg cgctctggtc gaaaagaagg gataggattc
    36001 gaacgaggaa cttgccagag aactggctga tatcatttac tacaccgtcg caatcgtagc
    36061 tattaatcat attgacctta ccaagactgt ctttgagaaa gacaaaaccg cagccgttaa
    36121 ttaccaacat aaacataatt tggaaggatt attgaaggga aagtagagat attgtcccct
    36181 tcctccaact tgagaaaatc cttggaacgt gataaaataa aggataagga tttttaataa
    36241 ttcattatat agaatcagtg gatttcttta tgaagaggtt tgaagtatct acagaaattg
    36301 gtagcctctc tgttacttat caaaagcaaa agaaagtgct agtttgtcta aacggagcag
    36361 gtttgctacc aagttatgaa aatttttcac ttatacttga aaaacctcct cctacaattg
    36421 gttatttgac aattgatttt ccgaacacag gtaggagtcc gattcatgac caagctggaa
    36481 aaaatctaga taatcttgca gatgcggttt atgaagtact tgaagaatta ggaatttctg
    36541 aatatatact ttgtgcacat agttggagtg gaattttagc ttgcaaatta ctcgagaaac
    36601 caattaagcg tcagacttta gtagcaattg aaccgacaac taaaaaagtc atgtttgctg
    36661 atttttcaga aaatccttat ccagaaatgg aagagcagat gaggctgatt gacgagtgtg
    36721 gtcctgaact ttattttaag aacttaactc aagcaacatt tagtcctgaa acgaataaaa
    36781 aaatctggga attaatgcaa gaaaaaggct tagagttgga aaatcaagat ccagaatttc
    36841 agatatctgg agagattact gaggaagatt ttgaggatgt atctatagaa gctcatattc
    36901 ctgtatttgt tttttgtcag ccttatagag aaaaagagta cagagaatca gaatattgga
    36961 cttccaatac taaactcatt ttaggaggga atcaccatta tttacaatgg tcagaatcgg
    37021 aaaaaattgc ggctattatt cgagaattgt tagaataaga tggaaaaaag gagattacag
    37081 gagacaagat gaactacttt aatgttggga aaatcgttaa tacgcaggga ttacagggtg
    37141 agatgcgagt cttgtctgtg acggattttg cagaagaacg gtttaaaaaa ggagctgagc
    37201 tggctttgtt tgatgaaaaa gatcagtttg tccaaacagt gaccatcgct agccaccgta
    37261 aacagaagaa ctttgacatt attaaattca aagatatgta ccatatcaat actatcgaaa
    37321 agtacaaggg atacagtctc aaggtcgctg aggaagattt gaatgaccta gacgatggtg
    37381 aattttacta tcacgagatt atcggtttgg aagtctatga gggtgatagc ttggttggaa
    37441 ccatcaagga aatcctgcaa ccaggtgcta atgatgtctg ggtggtcaaa cgaaaaggca
    37501 aacgtgattt gcttttacct tatatcccac cagtggttct caatgttgat attccaaata
    37561 aacgggtcga tgtggaaatc ttagaagggt tagacgatga agattgatat tttaaccctc
    37621 tttccagaga tgttttctcc actggagcac tcaatcgttg gaaaggctcg agaaaaaggg
    37681 ctcttggata tccagtatca taattttcga gaaaatgctg aaaaggcccg tcatgtagat
    37741 gatgagccct acggaggcgg tcagggcatg ttgctcagag tacaacctat tttcgattcc
    37801 tttgatgcta ttgaaaagaa aaatccgcgc gttattctcc tcgatcctgc tggaaagcag
    37861 tttgatcagg cttatgctga agatttggct caagaggaag agctaatctt tatctgtggg
    37921 cactatgagg gttatgatga gcgcattaag accttggtaa cagatgagat ttccctaggc
    37981 gactatgtcc tcactggtgg agaattggca gctatgacca tgattgatgc tacagttcgc
    38041 ctgattccag aagtgattgg caaggagtct agccaccaag atgatagttt ttcttcaggt
    38101 cttttagaat atcctcagta cacacgtcct tatgattatc gaggcatggt cgtgccagat
    38161 gtattgatga gtggccacca tgaaaagatt cgtcagtggc gattgtacga gagtttaaag
    38221 aaaacctacg agcgcagacc ggatttactt gaacattatc aactgacagt agaagaagaa
    38281 aaaatgctgg cagaaatcaa agaaaacaaa gaataaagga gaaacctatg caagtaatca
    38341 aacgtaatgg cgaaattgct gaatttaatc cagataagat ttaccaagcc atcttgaagg
    38401 cagcccaaac tgtttatgtt ttgacagatg atttgcgtca aaatcttgct caagtcacta
    38461 agaaggtagt tttggattta caagaagcca aggtggaacg tgcgactatc agtatgattc
    38521 aatctatggt tgaacatcgt ttattgggcg caggttacat taccattgca gaacactaca
    38581 tttcctatcg tttacaacgt gacttggaaa gaagtggtta tggagatcat atcgcagttc
    38641 atttacattt tgaacaaatt cgctaagaaa aaagagtggg atgaagcaac tacatctcac
    38701 tctttcttaa atctattttt ttgggctgtt tggacagttg aggggacaaa tcgcaggcgt
    38761 tgaatatcgc ttatacggat aatccggctc acatttttgg aaaaattttt cacgataatt
    38821 tgctggcctt gctgatcata tttgatgatg tcacctgtaa agcttgtctc agataagata
    38881 aggtgaacag cggtttgttt ctggatagcc tcttcaatag tggctgtaag ggagttggtt
    38941 tcagtctggt caggttggtc atatccattt aaaaaatcat gtattttatt tagaacttgc
    39001 tggaatttat gtctcatatt ggcctccctt cttttatatc aatattatat aaaattttcc
    39061 taaaatctac aagattttac gaataaacta atgaaactaa aaaaggagaa gaaaatggca
    39121 gaatttacat ttgaaatcga agagcacttg ttgactcttt ctgaaaacga aaaaggttgg
    39181 accaaggaaa ttaaccgtgt gagctttaat ggtgcccctg caaagtttga tattcgtgct
    39241 tggagtccag accatactaa aatgggcaaa gggattactc tctcaaatga agaatttcaa
    39301 acgatggtgg atgcctttaa aggcaactaa tactcttcga aaatctcttc aaaccgcgtc
    39361 agctttgcct tgccgtatgt atggttactg acttcgtcag tttcatccac aacctcaaaa
    39421 caatgttttg agctgacttc gtcagtttca tccacaacct caaaacaatg ttttgagctg
    39481 acttcgtcag tttcatccac aacctcaaaa cagtgttttg agcagcctgc ggctagcttc
    39541 ctagtttgct ctttgatttt cattgagtat aagttttcaa aataaaagaa aaggatattg
    39601 agtcatgata actcggtatc cttttttagt tagtaaagta agcctgattt ttaaggcgtt
    39661 gaagtagggg acggctaata aaactaatag ccagaagttt gccaaggtct ggaatgataa
    39721 agggaagaac ccccacaaca agagcttttt caaatgccat tccagctagg aaatgcaagc
    39781 tgagaatccc gccgacaaag acaagggcat cacccaagag gtttgcaaga aaaatcttaa
    39841 caacaccact cttgctgttg gttagagagg aagtaagtcc agagtaaacg agataaaacc
    39901 aaagatagcc tgcagtaggg ccaactaaag cctgaaaacc agctccacct cctgcaaaga
    39961 caggaagacc gatagcacct agaagaagat agagtccagc agaaagtaca gcctctctcg
    40021 gtctaaagac agtagcaatc aagccgattg caaagttttg cagagtgaag ggaacaggtc
    40081 caattggaag actgatttgt gccaaagcag caatgagagc agccccaata gcagggatag
    40141 cataaacgtg agcttttttc aaaatagtta acctcttttc taatgtatag taactattat
    40201 actcatatag gagtcgatgt caaccatttt ttaaaaacaa ggtaacaaaa actgtaacag
    40261 ccgattttcg tgactagtca agttggataa tttttttgtt gattcgaaaa tcattaaaaa
    40321 agaagcagac atatttatat cagcttcttt tgtttcgatt aacgcatggt tacaaattct
    40381 tcagatgcag ttgggtgaat tgccacagtt gcatcaaagt cagccttggt tgctcccatt
    40441 ttgatagcaa cggcaaatcc ctgaatcatt tcatcaacgc cgtagccaat tccatgaagt
    40501 ccgacaactt tttcttctga accagctgtt atgagtttga aacgggattc ttgacggttg
    40561 cgagtgcaag cagagtacat agatgcaaag cttgatttat aaaccttgat ttggtcttga
    40621 ccgtattctt taatagcttg ctcttctgtc aaaccaacag ttccgatagc agggtgtgaa
    40681 aagacaacag ttggaatagt tgagtaatcc atttttgcag tagtttttcc gttaaagaga
    40741 cgttcagata gggtacgtcc ggccttgatt gcaactggag tcagttcttt ctcgcccgtt
    40801 acatcaccta gagcatagag tccctcaaca acagtatttt ggtattcatc cacttggata
    40861 aagccacgtt cgttcagagt cactccagct ttttcaagtt gcaagccctt aacgtttgga
    40921 cggcggcctg tagcccagat aacttggcta gctgtgtgac tagtaccatc ttcgaaatga
    40981 atggtaatgc cgtcagtagt tttttctaac ttgacaggga ctttgtgagt gtgaagtggt
    41041 aagtttgttc tttccatttc cttgaccaaa ccttcaacga tgtaggaatc aaaaccacgt
    41101 aaaggacgat cgcggcgaac aaagagatct gtcttgacac caaaagtgtg gagtacgcca
    41161 gctaattcaa cggcgatata accagcgcct agaatggcaa ctgactctgg aagttcttcc
    41221 caggcaaata catcatcaga agagccacct agctcagcac caggaatatt aggaatactt
    41281 ggatgggcac ctgtagcaat cacgatatgt ttagcacgaa tcagttcacc atttacgctt
    41341 acagtatgag aatctacaaa ttcagcatga ccttcaatca agtctacacc gttgcgttta
    41401 aaactaccat cataagaaga acgagcgcga tcaatgtagg cttcacgatt gcgacgtagg
    41461 gttgcaaagt caaagttaag atcagtagtc ttaaagccgt agtcttctcc aaattgatgg
    41521 aaagtctcag cgatttgcgc cccgtaccac atgatttttt taggaacaca accgacgttg
    41581 acacaggttc cacctaattt cttttcctca ataacggctg ctttggctcc atgttcacca
    41641 gcacggttca tggtagcgat tcctccgcta cctccaccga tagcaatgat atcatattct
    41701 ctcattgaaa actcctttgt aatcttttaa atagtagagc gtcttttttg atagttatat
    41761 tttattcttt ttttgatttg attgcaaaaa tctgctacgc acaaaaaatt gcaaaaaagc
    41821 ttgccaaata agtcaagata gtgtattata tatctagtac aatagaaaag caaaattccg
    41881 aacgattgtt taatcctgaa ctgccattat ttccatctga actgcattgt ttgctggagg
    41941 gaaatttggt ataatagcaa agtattgtaa aaaagaaaag gagtttcatt atgaagaaaa
    42001 agaatggtaa agctaaaaag tggcaactgt atgcagcaat cggtgctgcg agtgtagttg
    42061 tattgggtgc cggggggatt ttacttttta gacaaccttc tcagactgct ctaaaagatg
    42121 agcctactca tcttgttgtt gccaaggaag gaagcgtggc atcctctgtt ttattgtcag
    42181 ggacagtaac agcaaaaaat gaacaatatg tttattttga tgctagtaag ggtgatttag
    42241 atgaaatcct tgtttctgtg ggcgataagg tcagcgaagg gcaggcttta gtcaagtaca
    42301 gtagttcaga agcgcaggcg gcctatgatt cagctagtcg agcagtagct agggcagatc
    42361 gtcatatcaa tgaactcaat caagcacgaa atgaagccgc ttcagctccg gctccacagt
    42421 taccagcgcc agtaggagga gaagatgcaa cggtgcaaag cccaactcca gtggctggaa
    42481 attctgttgc ttctattgat gctcaattgg gtgatgcccg tgatgcgcgt gcagatgctg
    42541 cggcgcaatt aagcaaggct caaagtcaat tggatgcaac aactgttctc agtaccctag
    42601 agggaactgt agtcgaagtc aatagcaatg tttctaaatc tccaacaggg gcgagccaag
    42661 ttatggttca tattgtcagc aatgaaaatt tacaagtcaa gggagaattg tctgagtaca
    42721 atctagccaa cctttctgta ggtcaagaag taagctttac ttctaaagtg tatcctgata
    42781 aaaaatggac tgggaaatta agctatattt ctgactatcc taaaaacaat ggtgaagcag
    42841 ctagtccagc agccgggaat aatacaggtt ctaaataccc ttatactatt gatgtgacag
    42901 gcgaggttgg tgatttgaaa caaggttttt ctgtcaacat tgaggttaaa agcaaaacta
    42961 aggctattct tgttcctgtt agcagtctag taatggatga tagtaaaaat tatgtctgga
    43021 ttgtggatga acaacaaaag gctaaaaaag ttgaggtttc attgggaaat gctgacgcag
    43081 aaaatcaaga aatcacttct ggtttaacga acggtgctaa ggtcatcagt aatccaacat
    43141 cttccttgga agaaggaaaa gaggtgaagg ctgatgaagc aactaattag tctaaaaaat
    43201 atcttcagaa gttaccgtaa tggtgaccaa gaactgcagg ttctcaaaaa tatcaatcta
    43261 gaagtgaatg agggtgaatt tgtagccatc atgggaccat ctgggtctgg taagtccact
    43321 ctgatgaata cgattggcat gttggataca ccaaccagtg gagaatatta tcttgaaggt
    43381 caagaagtgg ctgggctggg tgaaaaacaa ctagctaagg tccgtaacca acaaatcggt
    43441 tttgtctttc agcagttctt tcttctatcg aagctcaatg ctctgcaaaa tgtagaattg
    43501 cccttgattt acgcaggagt ttcgtcttca aaacgtcgca agttggctga ggaatattta
    43561 gacaaggttg aattgacaga acgtagtcac catttacctt cagaattatc tggtggtcaa
    43621 aagcaacgtg tagccattgc gcgtgccttg gtaaacaatc cttctattat cctagcggat
    43681 gaaccgacag gagccttgga taccaaaaca ggtaaccaaa ttatgcaatt attggttgat
    43741 ttgaataaag aaggaaaaac cattatcatg gtaacgcatg agcctgagat tgctgcctat
    43801 gccaaacgtc agattgtcat tcgggatggg gtcatttcgt ctgacagtgc tcagttagga
    43861 aaggaggaaa actaagatgc agaatctgaa atttgccttt tcatctatca tggctcacaa
    43921 gatgcgttct ttgcttacta tgattgggat tattatcggt gtttcatcag ttgttgtgat
    43981 tatggctttg ggtgattccc tatctcgtca agtcaataaa gatatgacta aatctcagaa
    44041 aaatattagc gtcttttttt ctcctaaaaa aagtaaagac gggtctttta ctcagaaaca
    44101 atcagctttt acggtttctg gaaaggaaga ggaagttcct gttgaaccgc caaaaccgca
    44161 agaatcctgg gtccaagagg cagctaaact gaagggagtg gatagttact atgtaaccaa
    44221 ttcaacgaat gccatcttga cctatcaaga taaaaaggtt gagaatgcta atttgacagg
    44281 tggaaacaga acttacatgg acgctgttaa gaatgaaatt attgcaggtc gtagtctgag
    44341 agagcaagat ttcaaagagt ttgcaagtgt cattttgcta gatgaggaat tgtccattag
    44401 tttatttgaa tctcctcaag aggctattaa caaggttgta gaagtcaatg gatttagtta
    44461 ccgggtcatt ggggtttata ctagtccgga ggctaaaaga tcaaaaatat atgggtttgg
    44521 tggcttgcct attactacca atatctccct tgctgcgaat tttaatgtag atgaaatagc
    44581 taatattgtc tttcgagtga atgataccag tttaacccca actctgggtc cagaactggc
    44641 acgaaaaatg acagagcttg caggcttaca acagggagaa taccaggtgg cagatgagtc
    44701 cgttgtattt gcagaaattc aacaatcgtt tagttttatg acgacgatta ttagttccat
    44761 cgcagggatt tctctctttg ttggaggaac tggtgtcatg aacatcatgc tggtttcggt
    44821 gacagagcgc actcgtgaga ttggtcttcg taaggctttg ggcgcaacac gtgccaatat
    44881 tttaattcag tttttgattg aatccatgat tttgaccttg ttaggtggct taattggctt
    44941 gacaattgca agtggtttaa ctgccttagc aggtttgtta ctgcaaggtt taatagaagg
    45001 tatagaagtt ggagtatcaa tcccagttgc cctatttagt cttgcagttt cggctagtgt
    45061 tggtatgatt tttggagtct tgccagccaa caaggcatcg aaacttgatc caattgaagc
    45121 ccttcgttat gaatgagatc aacaagatgg acactcgtct atcttgtttt tgtatggatt
    45181 tccctatcga aattcattaa aaatgtgata taatgaagtg ttagaaaaac aggtttcatt
    45241 tgcctggaaa ggaaaaatta tgtctgaaaa aaatttttat attacaacgc cgatttacta
    45301 tccatctggg aaacttcata tcggttctgc ctacacaact atcgcatgtg atgtcctagc
    45361 acgttacaaa cgcctgatgg gctacgatgt attttatctg acaggtcttg atgaacatgg
    45421 tcagaaaatc cagcagaaag cggaagaagc tggtattaca cctcaagcct atgttgatgg
    45481 aatggcggtt ggagttaaag aactctggca attactagat atctcatacg ataaatttat
    45541 ccgtacaact gatgactacc atgaaaaagt tgttgcacag gtctttgaac gcttacttgc
    45601 tcaagatgat atctacttgg gtgaatactc tggttggtat tcagtatcag acgaagaatt
    45661 ctttacagaa agccagctgg cagaagtttt ccgtgatgaa gctggaaatg tgactggtgg
    45721 tattgctcca tcaggtcatg aggttgaatg ggtttctgaa gaatcatact tccttcgcct
    45781 tagcaaatac caagaccgtt tggtcgaatt tttcaaagct catcctgaat ttatcacgcc
    45841 agatggtcgc cttaatgaaa tgctacgcaa cttcatcgag ccaggtttgg aagatttggc
    45901 ggtatctcgt acaaccttta catggggagt gcctgtccca tcaaatccaa aacacgttgt
    45961 ctacgtttgg attgatgccc ttcttaacta tgcgacagct cttggctacg ctcaagacga
    46021 acatggtaac tttgacaagt tctggaatgg aacagtcttc catatggtag gaaaagatat
    46081 ccttcgcttc cactctatct actggccaat ccttcttatg atgttggatg ttaaattacc
    46141 tgatcgtttg attgcccatg gttggtttgt catgaaagac ggaaaaatgt ctaagtcaaa
    46201 agggaatgtc gtttaccctg aaatgttggt agagcgttat ggactagatc cacttcgtta
    46261 ctacctcatg cgtaaccttc cagttggttc agacggaacc tttactcctg aagactatgt
    46321 cggccgtatc aactatgaat tggctaatga ccttgggaac ctccttaacc gtacggtttc
    46381 catgattaat aagtactttg atggacaaat ccctgcctat gtagaaggtg tgactgaatt
    46441 tgatcatgtt cttgctgagg ttgcagaaca atcaatcgca gacttccata cacacatgga
    46501 agcagttgac tatccacgtg cgcttgaagc agtctggact ctgatctctc gtaccaataa
    46561 atacatcgat gagactgcac catgggtctt ggacaaggat gaagctcttc gtgaccaatt
    46621 ggcaagtgtc atgagccact tggcagccag cattcgtgta gttgctcact tgattgaacc
    46681 atttatgatg gaaactagtc gtgcagtttt gactcaactt ggtttggaag aagtttctag
    46741 tcttgaaaac ttgagtttgg ctgacttccc agcagatgtg actgtagttg ccaaaggaac
    46801 acctatcttt ccacgtctaa atatggaaga agaaatcgcc tatatcaagg aacaaatgga
    46861 aggcaataaa ccagcagtcg aaaaagaatg gaatccggac gaagttgagc tcaaactaaa
    46921 caaggatgaa atcaagtttg aagactttga caaggttgaa atccgtgtcg cagaagtcaa
    46981 agaagtgtct aaagtagaag gttcagataa gttgcttcaa ttccgcttgg atgctggtga
    47041 tggagaagat cgtcagattc tttcaggaat tgcaaaatac tatccaaatg aacaagaatt
    47101 ggtcggcaag aaagttcaaa tcgttgctaa cctcaaacca cgtaaaatga tgaaaaaata
    47161 tgtcagccag ggtatgattc tctcagctga acatgatggc aaattacccc ttctcacagt
    47221 tgatccagct gtaccaaatg gaagtgtgat tgggtaaaag tagacaggac tagttcaggc
    47281 tagttctgtt tttttattta tactcaatga aaatcaaaga gcaaactagg aagctagccg
    47341 cgggttgatc aaagcactgc tttgaggttg tagatgaaac tgacgaagtc agctcaaagc
    47401 actgctttga ggttgtagat gaaactga
//
LOCUS       DAVKQB010000009        44903 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKQB010000009 DAVKQB010000000
VERSION     DAVKQB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44903
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.009"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            100..597
                     /locus_tag="VW528_000508"
     CDS             100..597
                     /locus_tag="VW528_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9911380.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            597..2252
                     /gene="dnaX"
                     /locus_tag="VW528_000509"
     CDS             597..2252
                     /gene="dnaX"
                     /locus_tag="VW528_000509"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9911381.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATDKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2280..2474
                     /locus_tag="VW528_000510"
     CDS             2280..2474
                     /locus_tag="VW528_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9911382.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2624..3394
                     /gene="sufC"
                     /locus_tag="VW528_000511"
     CDS             2624..3394
                     /gene="sufC"
                     /locus_tag="VW528_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9911383.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3422..4684
                     /gene="sufD"
                     /locus_tag="VW528_000512"
     CDS             3422..4684
                     /gene="sufD"
                     /locus_tag="VW528_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9911384.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4695..5921
                     /locus_tag="VW528_000513"
     CDS             4695..5921
                     /locus_tag="VW528_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9911385.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATAFYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVINPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5908..6348
                     /locus_tag="VW528_000514"
     CDS             5908..6348
                     /locus_tag="VW528_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9911386.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6402..7814
                     /gene="sufB"
                     /locus_tag="VW528_000515"
     CDS             6402..7814
                     /gene="sufB"
                     /locus_tag="VW528_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9911387.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8045..9286)
                     /locus_tag="VW528_000516"
     CDS             complement(8045..9286)
                     /locus_tag="VW528_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9911388.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKTPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9389..10498
                     /locus_tag="VW528_000517"
     CDS             9389..10498
                     /locus_tag="VW528_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9911389.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11032..11379
                     /gene="ssrA"
                     /locus_tag="VW528_000518"
     tmRNA           11032..11379
                     /gene="ssrA"
                     /locus_tag="VW528_000518"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11552..11639)
                     /locus_tag="VW528_000519"
     tRNA            complement(11552..11639)
                     /locus_tag="VW528_000519"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11603..11605),aa:Ser,seq:gga)
     gene            11802..12542
                     /locus_tag="VW528_000520"
     CDS             11802..12542
                     /locus_tag="VW528_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9911390.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12539..13450
                     /gene="pfkB"
                     /locus_tag="VW528_000521"
     CDS             12539..13450
                     /gene="pfkB"
                     /locus_tag="VW528_000521"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9911391.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTVEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13447..15399
                     /locus_tag="VW528_000522"
     CDS             13447..15399
                     /locus_tag="VW528_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9911392.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSAGQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15563..17788
                     /locus_tag="VW528_000523"
     CDS             15563..17788
                     /locus_tag="VW528_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9911393.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKTLLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(17833..18261)
                     /locus_tag="VW528_000524"
     CDS             complement(17833..18261)
                     /locus_tag="VW528_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911394.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITAGITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            18399..19541
                     /locus_tag="VW528_000525"
     CDS             18399..19541
                     /locus_tag="VW528_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9911395.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSVQPIEAI
                     SKLLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19550..20764
                     /gene="thiI"
                     /locus_tag="VW528_000526"
     CDS             19550..20764
                     /gene="thiI"
                     /locus_tag="VW528_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9911396.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGRAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            20899..21722
                     /locus_tag="VW528_000527"
                     /pseudo
     CDS             20899..21722
                     /locus_tag="VW528_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            21744..22483
                     /locus_tag="VW528_000528"
                     /pseudo
     CDS             21744..22483
                     /locus_tag="VW528_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            22496..23662
                     /locus_tag="VW528_000529"
     CDS             22496..23662
                     /locus_tag="VW528_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9911397.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     ATTFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            24170..25663
                     /locus_tag="VW528_000530"
     CDS             24170..25663
                     /locus_tag="VW528_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9911398.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            25676..26797
                     /locus_tag="VW528_000531"
     CDS             25676..26797
                     /locus_tag="VW528_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9911399.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     FQLDELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHL
                     RINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLA
                     LQNEFADFVVQVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            26857..27093
                     /locus_tag="VW528_000532"
     CDS             26857..27093
                     /locus_tag="VW528_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9911400.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            27090..27503
                     /locus_tag="VW528_000533"
     CDS             27090..27503
                     /locus_tag="VW528_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9911401.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            27621..28586
                     /locus_tag="VW528_000534"
     CDS             27621..28586
                     /locus_tag="VW528_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9911402.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            28610..29136
                     /locus_tag="VW528_000535"
                     /pseudo
     CDS             28610..29136
                     /locus_tag="VW528_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            29166..32516
                     /locus_tag="VW528_000536"
     CDS             29166..32516
                     /locus_tag="VW528_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9911403.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFGENCCVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIEEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            32787..32864
                     /locus_tag="VW528_000537"
                     /pseudo
     CDS             32787..32864
                     /locus_tag="VW528_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(33102..33572)
                     /locus_tag="VW528_000538"
     CDS             complement(33102..33572)
                     /locus_tag="VW528_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9911404.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(33589..35862)
                     /locus_tag="VW528_000539"
     CDS             complement(33589..35862)
                     /locus_tag="VW528_000539"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9911405.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHINKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            36209..39310
                     /locus_tag="VW528_000540"
     CDS             36209..39310
                     /locus_tag="VW528_000540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9911406.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQKSEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQVDD
                     SKKKLDVTLFSDLYRQVGQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHDSDQEMSRILDQYKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNGIVMKT
                     IYR"
     gene            39392..40399
                     /gene="pfkA"
                     /locus_tag="VW528_000541"
     CDS             39392..40399
                     /gene="pfkA"
                     /locus_tag="VW528_000541"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9911407.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTATDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     L"
     gene            40458..41963
                     /gene="pyk"
                     /locus_tag="VW528_000542"
     CDS             40458..41963
                     /gene="pyk"
                     /locus_tag="VW528_000542"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9911408.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(42697..42922)
                     /locus_tag="VW528_000543"
                     /pseudo
     CDS             complement(42697..42922)
                     /locus_tag="VW528_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            43770..44642
                     /locus_tag="VW528_000544"
     CDS             43770..44642
                     /locus_tag="VW528_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9911409.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            44655..44819
                     /locus_tag="VW528_000545"
     CDS             44655..44819
                     /locus_tag="VW528_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911410.1"
                     /translation="MTVILGFLFLFLMTWWVISIVNSQKPLKNETFIGYVQRYDIDGN
                     GYQSLPCPQN"
     gene            complement(44788..44903)
                     /locus_tag="VW528_000546"
                     /pseudo
     CDS             complement(44788..44903)
                     /locus_tag="VW528_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT        13718 a         7992 c         9719 g        13474 t
ORIGIN      
        1 ggggttaccc actacaaata ttatagagcc caaaatcctt tgtttactaa acaagggatt
       61 tttcttttgt ctctgctcct tttttgatat aatagttcta tgttaaaatc agaaaaacaa
      121 tcacgttatc aaatgttaaa tgaagaattg tccttcctat tggaaggcga aaccaatgtt
      181 ttggctaatc tttccaacgc cagtgctctc ataaaatcac gttttcctaa taccgtattt
      241 gcaggctttt atttgttcga tggaaaggaa ttggttttag gccccttcca aggaggtgtt
      301 tcctgcatcc gtattgcact aggcaagggt gtttgtggtg aggcagctca ctttcaggaa
      361 actgttattg ttggagatgt gacgacctat ctcaactata tttcttgtga tagtctagct
      421 aaaagtgaaa ttgtggtgcc gatgatgaag aatggtcagt tacttggagt tctggatctg
      481 gattcttcag agattgagga ttacgatgct atggatcgag attatttgga acaatttgtc
      541 gctattttgc ttgaaaagac aacatgggac tttacgatgt ttgaggaaaa atcttaatgt
      601 atcaagcact ttatcgaaaa tatagaagtc aaaacttctc ccagttagtt ggtcaagaag
      661 ttgtggctaa gactcttaaa caagcggtgg agcaagagaa aataagtcac gcttatcttt
      721 tttctggtcc tcgtggaacg ggaaaaacca gtgttgctaa aatctttgcc aaggctatga
      781 actgtcccaa tcaagtgggt ggcgaacctt gcaataactg ctatatttgt caagcagtga
      841 cggacggtag tttagaagat gtcattgaaa tggatgcagc ttctaataat ggggtagatg
      901 aaattcgcga aattcgtgat aaatctacct atgcgcctag ccttgctcgt tataaggttt
      961 atatcataga tgaggttcac atgctgtcta caggggcttt taatgccctc ctaaagacgc
     1021 tggaagaacc aacacagaat gtagtcttta ttttggccac tactgaattg cacaagattc
     1081 ctgctactat tctatcccgt gtgcaacgtt ttgagtttaa atcaattaag acacaggata
     1141 ttaaggaaca tattcactat atcttagaaa aagaaaatat cagttctgaa ccagaggctg
     1201 tggaaatcat tgccagacgg gcggaaggtg gaatgcggga cgccttgtct attttggatc
     1261 aagccctgag tttgacacag ggaaatgagc tgacgactgc tatctctgaa gaaattactg
     1321 gcaccattag cctatcagcc ttggatgatt atgtggcggc cttgtctcaa caggatgttc
     1381 ccaaagcttt gtcttgcttg aatcttcttt ttgacaatgg taagagcatg actcgttttg
     1441 tgaccgatct tttgcactat ttaagagact tgttaattgt tcaaacaggg ggagaaaata
     1501 ctcatcatag ttcagtcttt gtagaaaatt tggcacttcc tcaaaaaaat ctgtttgaaa
     1561 tgattcgctt agcaacagtg agtttagcag atattaagtc tagtttgcag cccaagattt
     1621 atgctgaaat gatgaccgtc cgtttggcgg aaatcaagcc cgaaccagct ctatcaggag
     1681 cggttgaaaa tgaaattgct acgctgagac aggaagttgc ccgtctcaaa caagagcttt
     1741 ctaatgtagg tgcggttcct aaacaagttg caccagctcc tagtcgacca gctacggaca
     1801 aaacagtcta tcgtgtcgat cgcaataaag tgcaatctat cttacaagag gccgtcgaaa
     1861 atcctgattt agcacgtcaa aatctaattc gtttgcagaa tgcctgggga gaggtaattg
     1921 aaagtctagg tgggccggac aaggctctgc tagttggttc tcaaccggtt gctgccaatg
     1981 aacaccatgc tattcttgct tttgagtcta acttcaatgc tggtcaaact atgaaacgag
     2041 acaatctcaa taccatgttt ggtaatatcc tcagtcaggc ggcaggtttt tcacctgaga
     2101 ttttagctat ttccatggag gaatggaaag aagttcgcgc agccttttca gccaaagcca
     2161 aatcttctca aactgaaaaa gaagtagaag aaagcctgat tccagaagga tttgaatttt
     2221 tggctgataa agtgaaggta gaggaagact aaagaaagat ttcatgatac aataagttta
     2281 tgaataaaca acaatttatt attatggcgc tgtttacagc tgctgagacc tattttttca
     2341 atgaagcctg gatgactggc cgctatatta tggcagcctt ttgggcaatt ttactcttta
     2401 gaaatttccg agtcagttat gtgatgggca aaatcgttga tgtcatcgat cagcatttta
     2461 ataggaaaga ctagccctca gcttccagac aaaatcaaag ccttttaggc ttttttttgt
     2521 tatactagaa aagtatattt atagaatttt tgctctattt ctggggaaat cagacgtttt
     2581 tctagtaagt actgtaaaag ttttgaaaaa gaaaggaact atcatgtcag tattagagat
     2641 taaagatctt cacgttgaga ttgaaggaaa agaaatttta aaaggggtta acctgaccct
     2701 gaaaacagga gaaattgccg ctatcatggg accaaatggt acaggtaaat cgactctttc
     2761 tgccgctatc atgggaaatc caaactatga agtaactaaa ggtgaagttt tgtttgatgg
     2821 cgtaaacatc cttgagttgg aagtggatga gcgtgcgcgt atgggacttt tccttgctat
     2881 gcaataccca tcagaaatcc ctggaattac caatgctgag tttcttcgtg ccgctatgaa
     2941 tgcgggtaaa gaagatgatg agaagatttc agttcgtgag tttattacta agctagatga
     3001 aaaaatggaa ttgctcaaca tgaaagaaga aatggcagag cgttacctca acgaaggctt
     3061 ctctggtggt gagaaaaaac gcaatgaaat tcttcaactt ttgatgttgg agccaacatt
     3121 tgctcttttg gacgagattg actcaggtct tgatattgac gctcttaaag ttgtgtctaa
     3181 aggtgtcaat gccatgcgtg gtgaaggttt tggtgctatg atcatcactc actaccaacg
     3241 tcttttgaac tatatcacac ctgatgtggt acacgtgatg atggaaggtc gtgttgtcct
     3301 ttctggtggt ccagaattgg ctgcgcgttt ggaacgtgaa ggatacgcaa aattagctga
     3361 agaacttggc tacgactaca aggaagaatt gtaatttcct cgtatctttt aggagaagta
     3421 aatgactaga gaaaatatta aacttttttc agaaatgcac gctgaaccaa gctggttggc
     3481 tgatctccgt caaaaagctt ttgacaagat tgagactttg gaattaccag ttattgagcg
     3541 tgtcaaattc caccgttgga atctgggtga tggaacgatt acagaaaatg agccatcagc
     3601 aaatgttcca gatttcacag ctttagatca tcacttgaag ttggtgcaag taggaactca
     3661 aactgttttc gaacaaactc cagttgagtt agctgaacag ggtgttgtct tcacagactt
     3721 tcactcagct ttagaagaaa ttccagagct gatcgaagaa ttcttcatgt catctgttaa
     3781 gtatgatgat gacaagttgg cggcttacca cacagcttac tttaacagtg gtgctgtact
     3841 ctatattcca gataacgtag aaatcacaga gccaattgaa ggaattttct accaagatag
     3901 cgatagcaat gtgccgttta acaagcatat tatgattatc gctggtaaaa attctaagat
     3961 tagttatctg gagcgtttag agtcacgcgg tgaaggaagt gacaaagtaa ctgccaatat
     4021 cacagtggaa gtgattgcac gttctggtgc gcaagtcaag tttgctgcta tcgaccgtct
     4081 aggtgaaaac gtcactgcct acattagccg tcgtggtaaa ttaggcaacg atgcaagtat
     4141 tgactgggct atcggtgtca tgaacgaagg aaatgtcgtt gctgattttg atagtgactt
     4201 gattggtaat ggtagccatg ctgacctcaa ggttgtagct ctttcaagtg gtcgtcaggt
     4261 acaagggatt gatactcgtg taactaacta tggctgcaac tctatcggaa atatcctaca
     4321 acatggggtt atccttgaaa aagcaacttt gactttcaat ggtatcggcc acatcatcaa
     4381 gggtgctaag ggagcagatg cgcaacaaga gagccgtgtt ctcatgcttt cagaccaagc
     4441 gcgttcagat gctaacccaa ttcttttgat tgatgaaaat gatgtcactg caggacacgc
     4501 agcgtctatc ggacaagttg atccagaaga catgtattac ctcatgagtc gtggattgga
     4561 taaggcaact gcagagcgtt tggttgttcg tggtttcctt ggatctgtta tcgtggagat
     4621 tccagtcaag gaagttcgtg atgaaatgat tgcaactatc gaagagaaat tgtcaaaacg
     4681 ctaaggggaa gcctatgtta gatgtagaag cgattcgcaa ggattttcca attttagatc
     4741 agattgtcaa tgatgaacct ctggtctatc tggacaatgc tgcgacgaca caaaaacccc
     4801 tagtagttct gaaagctatt aacagttact atgagcagga caatgccaat gttcaccgtg
     4861 gtgtccatac cttagcggaa cgagcgacag ctttttatga agctgctcgt gaaaccattc
     4921 gtaagtttat taatgcaggc tctacaaagg aagttctctt taccagagga acgacaacca
     4981 gccttaactg ggtggcacgc tttgctgagg aaattctcac tgagggagac caggtcttga
     5041 tttcagtaat ggaacaccat tctaatatca ttccatggca ggaagcttgt cgaaagactg
     5101 gagcagagct tgtctatgtc tatcttaaag acggtgcctt ggatatggag gatttgcgag
     5161 ctaaattgac tgataaggtt aaatttgttt ccctagctca tgcctccaat gttcttggtg
     5221 tgatcaatcc gatcaaggaa atcactcaat tagcccacca agttggggca atcatggtag
     5281 tggatggtgc tcaatctaca cctcatatga agattgatgt ccaggacttg gatctggact
     5341 ttttcgcctt ttcgggtcac aagatggctg gtccgactgg tatcggtgtc ctttacggca
     5401 aagaaaagta tcttgagcaa atgtctccag tagaatttgg cggcgagatg attgattttg
     5461 tctacgagca atttgctagt tggaaggaat tgccttggaa atttgaggct ggaacgccaa
     5521 atatggcagg agctattgga cttgcgactg cagttgatta tttggaaaag attggtatgg
     5581 atgccgttga agctcatgaa caggaattga ttgcgtacgt ctatccaaaa ctgcaggcaa
     5641 ttgagggatt gaccatttac ggttctcagg atttggctca acgttcgggt gttattgcct
     5701 ttaacctagg tgatctccat cctcacgatc ttgcgacggc tctggattat gaaggagtgg
     5761 ctgttcgtgc tggtcaccat tgtgcgcaac ccttgcttca gtatttggaa gtcccagcaa
     5821 cagctcgtgc aagtttttat atctacaata ccaaggcaga ttgcgacaaa ctagtcgatg
     5881 ccctacaaaa gacaaaggag tttttcaatg gcactttcta aactagatag cctttatatg
     5941 gcagtggtag cagaccattc gaaaaatcca catcaccaag ggaagttaga agatgctgag
     6001 caaatcagtc tcaacaatcc gacttgtggg gatgtcatca acctctctgt caagtttgat
     6061 gcagaggacc gtttggaaga tattgctttt ctaaattcag gatgcacgat ttcaactgct
     6121 tctgctagta tgatgacaga tgccgtttta ggaaaaacca aacaagaaat tttagaactg
     6181 gcgactattt tttctgaaat ggttcaaggg caaaaagatg agcgtcaagg ccaacttgga
     6241 gacgcggcat tcttgtcagg tgttgccaaa ttccctcaaa gaatcaagtg tgcaacccta
     6301 gcttggaatg cccttaagaa aacaattgaa aatcaagaaa aacagtaaga caagtttctt
     6361 ttgtcttatg aattattaga aatgaagaaa gaaaggatac tatggctgaa gaaagagtag
     6421 aaccaaaacc aattgacctt ggtgaatata aatttggttt ccatgacgat gtagagcctg
     6481 tcttatcgac aggaaaagga ctcaacgaag gtgttattcg tgaattatct gctgctaagg
     6541 gtgagcctga gtggatgttg gagttccgtt tgaagtctta tgaaaccttc aaaaaaatgc
     6601 ccatgcaaac ttggggagca gacttgtcag agattgactt tgatgactta atctactacc
     6661 aaaaaccatc tgacaaacca gcccgttctt gggatgatgt acctgaaaag attaaagaaa
     6721 cctttgaacg tatcgggatt ccagaagctg aacgtgctta tttagcaggg gcttctgccc
     6781 agtacgagtc agaagtggtt taccacaaca tgaaggaaga gttccaaaaa ttaggtatta
     6841 tctttacaga tacagattcc gcactcaagg aatacccaga cttatttaaa caatactttg
     6901 cgaagttggt accgccgaca gataacaagt tggcagccct caactcagca gtatggtcgg
     6961 gtggaacttt tatctacgtg ccaaaaggtg tcaaggtaga tattccactt caaacttatt
     7021 tccgtatcaa taacgaaaat ataggtcagt tcgaacgtac cttgattatc gttgatgagg
     7081 gagcaagcgt ccactacgta gaaggatgta cagcaccaac atattcaagc aatagcttac
     7141 acgctgccat tgtagaaatt tttgctttgg acggagctta tatgcgttat acaactatcc
     7201 aaaactggtc tgataacgtc tataacttgg taacaaagcg tgctaaggct caaaaggatg
     7261 ccactgttga gtggattgat ggaaacttgg gtgccaaaac gactatgaaa tatccatctg
     7321 tttaccttga tggagaagga gcgcgtggta ccatgctctc tatcgccttt gctaatgcag
     7381 ggcaacacca agacacgggt gctaagatga ttcacaatgc tccacatacc agctcgtcta
     7441 ttgtgtctaa atccatcgct aaaggtggag gaaaggttga ctaccgtgga caagtcacct
     7501 ttaacaagaa ctctaagaaa tctgtttccc acattgaatg tgataccatt atcatggatg
     7561 acttgtcagc atcagatact attccattta atgaaattca caactcgcaa gtggctttgg
     7621 aacacgaagc caaagtatct aagatttcag aagagcaatt gtattatctc atgagccgtg
     7681 gattgtcaga atctgaggca actgaaatga ttgtcatggg atttgtagaa ccctttacaa
     7741 aagaacttcc aatggaatac gcagttgagc tgaaccgctt gattagctat gaaatggagg
     7801 gatcagttgg ataaaatttg attttatact cttcgaaaat ctcttcaaac cacgtcagca
     7861 tcgccttacc gtatgtatgg ttactgactt cgtcagtttc atctacaacc tcaaaacagt
     7921 gttttgagca agctgcggct agcttcctag tttgttcttt gattttgagt attagattta
     7981 ctcaaaatca aggattttga agatgaactt gtatcaaaaa atcgcggttt aaaatcgcga
     8041 ttttttataa tttctcgtta acaaagcgga caaactgatt ccaccaaact tttaagaaga
     8101 aggctttttc aattttcttg tctgctacca tttcgaaact agggcgctct gtggtgatgt
     8161 aaccttgacc aatcaagtcc ttgtcttcat aagtcaaatg gccaaccact gttccagctt
     8221 caagtggtgc tgggattgct ttggaatcag gtgtgaattg aacagattgg gaagattgat
     8281 tcccaacacg ttcgattaga tagatatcct ctggagccac tgcagttact gtatcttctt
     8341 ttccatcttg tacaggggtt ttgctatctt gataggcatc gccttgttga acgattttgc
     8401 gaagtgtaaa tgtagaagaa atataatcca ttagggaaga tgtagctgta aatcgagcgt
     8461 aaggattatt gtcttgatga tctgcattta aaacaactgt gatgactctc atgccttttt
     8521 cgacagtagt accaacaaaa gactctccag ccttatctgt tgttcctgtt tttagcccat
     8581 caaaaccacc acggtaagca ggcatacctt ctaacatgta gttggttgaa gtgattgtca
     8641 tcccagcaaa agtagaagaa ggttttttgg tgatttctaa gacttgtggg tattttttga
     8701 tgaggttgcg agcaacgata gcgacatcat aagcactaag cttattttcc tcatcttttt
     8761 tagaacctgg gtaaatgtta tcccctagag tttcattgtt aagacctgtc gtattgacaa
     8821 cagtggcatc ctgaattccc cattccaaga gttttgcccg catcatatcg acgaaatctt
     8881 tttctgagcc agcaattttc tcagctaggg caatagcggc gctgttggca ctagatacca
     8941 gagttgcttc aagcaactct tcgacagtat aattacgggc ctccatagga atattactgg
     9001 cttcagaatt tgtcgtcaat tgataaggat aatcagaaat atctacagga gtggagaggg
     9061 taatacttcc gttttccaaa gcttcataga ccagataaac agtaatcaat tttgttatgg
     9121 aagcaatttc gacaggttgc gttgcatcct tctcatagag aattttacca gtatttgcct
     9181 caacagcaat cgcatgttta gcggcaatgg taaaatcttg agcaacagca gtagaagcac
     9241 cccctaaaag agagacagtt aacaaagtta aaaatatttt tttcatagta gtcttattct
     9301 atcataaaga aaaaaaatat tcttgcttta ataattcatc tgttaagctt tttgaaaata
     9361 tggtaaaata aagtgaggga ggtaactcat gtttcgtaga aataaattat ttttttggac
     9421 cacagaaatt ttactcttaa ccatcatctt ttacctatgg agacagatgg gatctttgat
     9481 taaccctttt gttagcgtgc ttaatacaat tatgattcca tttttattag ggggctttct
     9541 ttattatttg acaaacccta ttgttacttt cttaaataaa gtctgtaaac tcaatcgttt
     9601 gcttggtatt ttaattacct tgtgtacttt ggtctgggga atggtcatag gtgttgtcta
     9661 tctcttacct attttgatta atcagttatc tagtttgatt atatctagtc aaactattta
     9721 tagtcgagta caagacttaa tcatagactt atctaattat cctgcgctcc agaatttgga
     9781 tgtagaagct acaattcagc agttaaactt atcctatgtt gatattcttc aaaatatcct
     9841 aaatagcgta tcaaatagtg tggggagcgt cttgtcagct cttatcagta ctgttttgat
     9901 tttgattatg actccagttt ttttggttta tttcttatta gatggacata aattcttgcc
     9961 catgcttgaa agaacgattc taaagaggga tcgcttgcat attgcaggct tattaaagaa
    10021 tttaaatgcg acgattgctc gctatattag tggagtttcg attgacgcaa tcattatagg
    10081 ttgtttggct tatattggct atagtattat tggtttaaaa tatgctttag tttttgccat
    10141 tttttctggt gtagccaatt taattcctta tgtggggcca agtattggtt tgattcctat
    10201 gatcatcgca aatatattca ctgatcccca tagactgctg attgcagtga tttatatgct
    10261 tgttgttcag caggtagatg gcaatatctt atatcctcga atcgtaggaa gtgttatgaa
    10321 ggttcatcca atcacgattt tagttttact tttgttgtca agcaatatct atggtgtagt
    10381 tggaatgatt gtcgcagtgc caacctattc tatcttgaaa gaaatttcta agttcttatc
    10441 ccgtttgtat gaaaatcata aaataatgaa agaacgagaa agagaattag ctaagtaaaa
    10501 gtcaggagaa ccctgatttt tctttactgg aagtggcctt tagattagaa gactgaaaat
    10561 aagttaaagt cttaaactaa ttttcacagc taagaatagt agaagttaat ctgataaaaa
    10621 tcgaaaaaac cagtggaatt ctgtgtcagg gtaagttcca ctggttttca tagtctatta
    10681 aagttcgaat gaaacctatt tatagtagat tgaaactaga atagtacacc tctaattcta
    10741 aaacattgtt agaaatcgat ttgactgtcc tgatctattc gtcctattct tatttcattt
    10801 tactatattt tggtgcaata agtgaaaagt agaccgaata atataaggat tgattttata
    10861 gtttttaaac tcaaatgaat tgaaataaag agagtacgaa aattctcatc tgaaagtatt
    10921 ttagaataat tctcttcgtg aatttcttca aaacagatag cttcatctta ggtatgtgat
    10981 ttctttttgc atttttgagt tagataaggt ataatggttt tattgtcttt tggggtcgtt
    11041 acggattcga caggcattat gaggcatatt ttgcgactcg tgtggcgacg taaacgctca
    11101 gttaaatata actgcaaaaa ataacacttc ttacgctcta gctgcctaaa aaccagcagg
    11161 cgtgacccga tttggattgc tcgtgttcaa tgacaggtct tattattagc gagatacgat
    11221 taagccttgt ctagcggttt gataagagat tgatagactc gcagtttcta gacttgagtt
    11281 atgtgtcgag gggctgttaa aataatacat aacctatggt tgtagacaaa tatgttggca
    11341 ggtgtttgga cgtgggttcg actcccaccg gctccattat tcctttgcat tccttggtaa
    11401 aacgttgtta aatcaacgtt ttttattttt atctttggta ttcctttgca ttcttttgct
    11461 aaaaagggag tcacaaacag accctatttt aaaaaaagga tagaaaaaag gatacaacat
    11521 ttgtcgcatc ctaaaaataa tcttttttcg acggaagaca tgggattcga acccacgcac
    11581 gctattacac gcctaccgcg tttccaacac ggcctcttaa gcctcttgag taatcttcca
    11641 atacttactc aaatagtcta ccataaaggc tcttatcttg caataaaaat tctagaaata
    11701 agaaaaaatg atagattttg aaagaaaatg ataaaaaatg cttgacttcg aaagaaagta
    11761 tgatagaatg aatagtgtaa acgataacag gaggtgattc agtgttaaaa acagaacgta
    11821 aacaactaat tttagaggag ttaaatcagc atcatgtagt ttctctagaa aaattagtta
    11881 gtttgctaga aacgtcagaa tcaacggttc gaagagactt ggatgagttg gaagcggaaa
    11941 acaagcttcg tcgtgtgcat ggtggagcag aactccccca ctccttacag gaagaagaaa
    12001 ccattcaaga aaaatctgtc aaaaaccttc aagaaaagaa gttgctggct cagaaagcag
    12061 cctctctcat taaagaaaaa gatgtcatct ttatcgatgc tggaacaaca actgcttttt
    12121 tgattcatga attggtcaat aagaatgtta cagttgtgac caactccatt caccatgccg
    12181 ctcagttggt tgaaaagcag attccaactg tcatggttgg aggaaacgtc aagacggcga
    12241 cagatgctag tatcgggggc gttgctctta accagattaa ccaattgcac tttgaccgtg
    12301 cctttatcgg aatgaatggt gttgacgatg gctattatac gactcctgat atggaggagg
    12361 gagctgtgaa aagagctatt ttggagaatg ccaagcagac ctacgtcttg gtggattcgt
    12421 caaaaattgg acaaacttgc tttgccaagg tagcaccact caaacgcgct atcgttatca
    12481 ctagtcaagg gcatgagctc ttgcaggtta ttaaggagaa aacggaggta atagaagtat
    12541 gatttataca gtcacactca atccatccat tgactatatc gttcgtttgg accaagtcaa
    12601 agttggtagt gtcaatcgta tggacagtga tgataagttt gctggtggga aaggaatcaa
    12661 tgtcagccgt gtcttgaaac gtttgaatat accaaataca gcgacgggat ttatcggtgg
    12721 ctttactggt aaatttatca cagatactct agcagaggaa gaaatcgaga cacgttttgt
    12781 ccaagtggca gaagatactc gtatcaatgt taaaatcaaa gcagaccaag aaacagaaat
    12841 caacggaacg ggtccaactg ttgaaccggt tcagctagaa gaattgaaag ctattttatc
    12901 tagtctgaca gtagaagata cagttgtctt tgcaggttca agtgctaaaa atctaggcaa
    12961 tgttatctat aaggatttga tttccttgac gcgccagact ggtgcgcaag tggtctgtga
    13021 ctttgaagga cagaccttaa ttgatagttt ggactaccag cctcttcttg taaaaccaaa
    13081 caatcatgaa cttggagcga tttttggggt taaactcgaa agtttagatg aaattgagaa
    13141 atacgctcgt gagttactgg ctaagggtgc tcaaaatgtt attatctcta tggctggtga
    13201 tggcgccctt cttgtcacat ctgagggagc ttacttcgct aaaccaatca aaggaacagt
    13261 caaaaattca gttggagctg gtgattctat ggttgctgga ttcacaggtg aatttgtcaa
    13321 atcaaaagac gcagtagaag ccttcaaatg gggagtggct tgcggaacgg caactacctt
    13381 ctcagatgac ttggcaacgg cggaatttat taaagaaaca tatggaaaag ttgaggtaga
    13441 aaaaagatga aaattcaaga cctattgaga aaagatgtca tgttgctaga tttgcaggca
    13501 actgaaaaaa cagctgtcat cgacgagatg attaaaaatt tgacagagca cggttatgta
    13561 acagattttg aaacatttaa agaaggaatt ttggcgcgtg aagctttgac ttctactggt
    13621 ttgggtgatg gaatcgcaat gcctcacagc aaaaacgctg ctgtcaaaga agcgacagtt
    13681 ctatttgcta agtcaaacaa gggtgttgac tacgagagct tggatggaca agcaactgac
    13741 ctcttcttca tgattgcagc tccagaaggt gccaatgata ctcacttggc agccttggca
    13801 gaattgtctc aatacttgat gaaagacggt tttgcagaca aacttcgtca agcaacatct
    13861 gcaggccaag ttatcgaact ttttgaccaa gcttcagaaa aaactgagga acttgttcaa
    13921 gcacctgcta atgactctgg tgactttatc gtagctgtta cagcttgtac aacaggtatt
    13981 gcccacactt acatggccca agaagccctt caaaaagtag ctgctgaaat gggggttggt
    14041 atcaaggtcg aaaccaacgg tgctagcggt gttggaaatc aactaactgc agaagatatc
    14101 cgtaaggcta aagctattat cattgcagca gacaaggccg ttgaaatgga tcgatttgat
    14161 ggaaaaccat tgatcaatcg tccagttgct gacggtatcc gtaagacaga agagctaatt
    14221 aacttggctc tttcaggaga tactgaagtc taccgtgccg ctaatggtgc caaagctgca
    14281 acagcctcta acgaaaaaca aagccttggt ggtgccttgt acaaacactt gatgagtggt
    14341 gtatctcaaa tgttaccatt cgttatcggt ggtggtatca tgattgccct tgccttcttg
    14401 attgacggtg ctttgggtgt tccaaatgaa aaccttggca atcttggttc ttaccatgag
    14461 ttagcttcta tgtttatgaa aattggtgga gctgcctttg gtttgatgct tccagtcttt
    14521 gcgggttatg ttgcctactc tattgctgaa aaaccgggtt tggtagcagg tttcgtggct
    14581 ggtgctattg ccaaagaagg ttttgccttt ggtaaaattc cttatgccgc aggtggtgaa
    14641 gcaacttcaa ctcttgcagg tgtctcatct ggtttcctag gtgcccttgt tggtggattt
    14701 atcgcaggtg ccttggttct tgccatcaag aaatacgtta aagttcctcg ttcactcgaa
    14761 ggtgctaaat caatccttct attgccactt cttggaacaa tcttgacagg atttgttatg
    14821 ctagctgtga atatcccaat ggctgcaatc aacactgcta tgaatgactt cctaggcggt
    14881 cttggaggag gttcagctgt ccttcttggt atcgtccttg gtggaatgat ggctgttgac
    14941 atgggtggac cagttaataa agcagcttat gtctttggta caggtacgct tgcagcaact
    15001 gtttcttcag gtggttctgt agccatggca gcagttatgg ctggaggaat ggtgccacca
    15061 cttgcaatct ttgtcgcaac tcttcttttc aaagataaat ttactaagga agaacgtaac
    15121 tctggtttga caaacatcat catgggcttg tcatttatca ctgagggagc gattccattt
    15181 ggtgccgctg acccagctcg tgcgattcca agcttcatcc ttggttcagc agtagcaggt
    15241 ggactcgttg gtcttactgg tatcaaactc atggcgccac acggaggaat cttcgttatc
    15301 gcccttactt caaatgctct cctttacctc gtttctgtct tggtaggagc aatcgtaagt
    15361 ggtgtggttt atggttacct acgcaaacca caagcataaa aaatagaaaa atgaaaagat
    15421 tggaccgttt ggtgcagtct ttttctcttc ccgaaatgcc tgtgaaatat ggtataatag
    15481 aagaatggca aacaagaata caagtacaac aagacggaga ccgtctaaag cagaactgga
    15541 aagaaaagaa gcgattcaac gaatgttgat ttcgttagga attgcgattt tattgatttt
    15601 cgcagccttc aaattagggg ctgcaggtat aaccctttat aatttaattc gcttgctagt
    15661 gggtagccta gcttatctgg cgatattcgg cctattaatc tatctcttct ttttcaagtg
    15721 gatacgaaaa caggaaggac tcttatctgg ctttttcacc atatttgctg gcttactctt
    15781 gatttttgag gcctacttgg tttggaaata tggtttggac aagtccgttc taaaagggac
    15841 catggctcag gttgtgacag atctgactgg ttttcgaacg actagctttg ctggaggggg
    15901 cttgatcggg gtcgctcttt atattccaac agcctttctc ttttcaaata tcggaactta
    15961 ctttattggt tctatcttga ttttagtggg ttctctccta gtcagccctt ggtctgttta
    16021 cgatattgct gaatttttca gtagaggctt tgccaaatgg tgggaagggc acgagcgtcg
    16081 aaaagaggaa cgctttgtca aacaagaaga aaaagctcgc caaaaggctg agaaagaggc
    16141 tagattagaa caagaagaga ctgaaaaaac cttactcgat ttgcctcctg ttgatatgga
    16201 aacgggtgaa attctgacag aggaagctgt tcaaaatctt ccacctattc cagaagaaaa
    16261 gtgggtggaa ccagaaatca tcctgcctca agctgaactt aaattccctg aacaggaaga
    16321 tgactcagat gacgaagatg ttcaggtcga tttttcagcc aaagaagccc ttgaatacaa
    16381 acttccaagc ttacaactct ttgcaccaga taaaccaaaa gatcaatcta aagagaagaa
    16441 aattgtcaga gaaaatatca aaatcttaga agcaaccttt gctagctttg gtattaaggt
    16501 aacagttgaa cgggccgaaa ttgggccatc agtgaccaag tatgaagtca agccggctgt
    16561 tggtgtaagg gtcaaccgca tttccaatct atcagatgac ctcgctctag ccttggctgc
    16621 caaagatgtc cggattgaag caccaatccc tgggaaatcc ctaatcggaa ttgaagtgcc
    16681 caactccgat attgccactg tatctttccg agaactatgg gaacaatcgc aaacgaaagc
    16741 agaaaatttc ttggaaattc ctttagggaa ggctgttaat ggaaccgcaa gagcttttga
    16801 cctttctaaa atgccccact tgctagttgc aggttcaacg ggttcaggga agtcagtagc
    16861 agttaacggc attattgcta gcattctcat gaaggcgaga ccagatcaag ttaaatttat
    16921 gatggtcgat cccaagatgg ttgagttatc tgtttacaat gatattcccc acctcttgat
    16981 tccagtcgtg accaatccac gtaaagccag caaggctctg caaaaggttg tggatgaaat
    17041 ggaaaaccgt tatgaactct ttgccaaggt gggagttcgg aatattgcag gttttaatgc
    17101 caaggtagaa gagttcaatt cccagtctga gtacaagcaa attccgctac cattcattgt
    17161 cgtgattgtg gatgagttgg ctgacctcat gatggtggcc agcaaggaag tggaagatgc
    17221 tatcatccgt cttgggcaga aggcgcgtgc tgcaggtatc cacatgattc ttgcaactca
    17281 gcgtccatct gttgatgtca tctctggttt gattaaggcc aatgttccat ctcgtgtagc
    17341 atttgcggtt tcatcaggaa cagactcccg tacgattttg gatgaaaatg gagcagaaaa
    17401 acttcttggt cgaggagaca tgctctttaa accgattgat gaaaatcatc cagttcgtct
    17461 ccaaggctcc tttatctcgg atgacgatgt tgagcgcatt gtgaacttca tcaaggctca
    17521 ggcagatgca gactacgatg agagttttga tccaggtgag gtttctgaaa atgaaggaga
    17581 attttcggat ggagatgctg gtggtgatcc gctttttgaa gaagctaagt ctttggttat
    17641 cgaaacacag aaagccagtg cgtctatgat tcagcgtcgt ttatcagttg gatttaaccg
    17701 tgcgacccgt ctcatggaag aactggagat agcaggtgtc atcggtccag ctgaaggtac
    17761 caaacctcga aaagtgttac aacaataaaa aaatagcttc tttccgagtt tggagggaag
    17821 ctattttagt ggctattgat tgcttttatt ttctgaagtt ggcgcattgg actgtttttc
    17881 gttttcagta gcaggtttac ttgaagcagg agtagaagag tcctgagttg ctgttttctg
    17941 atcttctttt ttctcttcct tgacgctaga ttttggtgtt tcctcttgct gtgttttttc
    18001 ttgactagtg ttagtctctt tagttggact ggtgttttcc ttaggggatt ccttttggat
    18061 ttctttgaca atggttgtcg tctggcttgt cgtaggttct tttttaatat ttttgttatt
    18121 atccaaggcg ttcatgatcg tgatacccgc ggtaatcaag ccaaggatac tagcgattgt
    18181 agcaacggtt ttttgaaaga ctgtaaagcc ttttttgatg tgttctgttt ttggttttga
    18241 agttctacga tagttagaca tacattctct cctttgatta tgatttatta tactctgttt
    18301 ctttaaaaaa atcttaaaaa aagagaaatt gccctttctt gtcgcaggct ccgtttcatg
    18361 atacaataga aggagtgatt ttagaaagcg tgagaaacat gatttacttt gataattcgg
    18421 cgacgaccag gccctatcca gaagcccttg aaacctatat gcaggtggct tcaaaaattt
    18481 taggaaatcc atctagtctc catcgtttgg gagaccaggc aacacgaatt ttagatgctt
    18541 cccgtcaaca gattgcagat ttaatcggta agaaaagtga tgaaatcttc tttacatctg
    18601 gtgggacaga aggggataac tggatcatca agggagtagc atttgaaaaa gcccagtttg
    18661 gcaagcacat catcgtatca gccattgaac atccagcagt taaggaatca gccctctggt
    18721 tgaaagctca aggttttgaa gtggattttg ctccagttga taacaaagga tttgtggatg
    18781 ttgaggcgct agaagatttg ataagacctg atacgaccct cgtttccatc atggcagtta
    18841 acaatgaaat tggctctgtt cagcccattg aggctatttc aaaactattg gcagacaagc
    18901 caactatttc cttccacgtt gatgcggttc aggcgcttgc caagattccg actgaaaagt
    18961 atctgacaga acgagtggat ttcgcgactt tctctggtca caagttccac ggaatccgtg
    19021 gtgttggttt tatctatatc aagtctggca agaagattac gcctctttta acaggtggtg
    19081 gtcaggaacg tgattaccgt tcgacaactg aaaatgtggc aggaattgca gcgaccgcta
    19141 aggctctccg tttatctatg gaaaagctag atatctttag gagcaagact gggcagatga
    19201 aggcagtgat tcgtcaagca cttctggact atccggatat atttgtcttt tcagatgagg
    19261 aagacttcgc ccctcatatt ctgacttttg gaatcaaggg tgttcgtggt gaggtcattg
    19321 ttcacgcctt tgaagactat gatattttca tctcaacgac ctcggcttgt tcgtccaagg
    19381 ctgggaaacc agccggaacc ctgattgcta tgggagtgga caaggataag gctcagtcag
    19441 ctgtgcgtct tagtctagac cttgaaaatg atatgagtca ggtcgagcag ttcttgacca
    19501 agctaaaatt aatttacaat caaactagaa aagtaagata ggagcattca tgcagtattc
    19561 agaaattatg attcgctacg gtgagctgtc aaccaagggc aaaaatcgta tgcgtttcat
    19621 caataaactt cgcaataata tttcagacgt tttgtctatc tatccccaag ttaaggtaac
    19681 agcagatcgc gaccgtgccc acgcttacct caatggagca gattacacag cagtagcaga
    19741 atcgctcaaa caagtatttg gaattcaaaa cttttcccct gtttataagg ttgaaaaatc
    19801 tgtagaagtt ctgaagtctg ctgtccaaga gattatgcag gacatctaca aggaaggcat
    19861 gacctttaaa atctctagca agcgtagcga ccacaccttt gaattggaca gtcgtgaact
    19921 caaccaaacg cttggaaggg ctgtattcga agccattcca aacgtacaag ctcaaatgaa
    19981 aagtcctgac atcaatcttc aggtggagat acgtgaagaa gcagcctatc tttcttatga
    20041 aaccgttcga ggggcaggtg gattaccagt gggaacttct ggtaagggca tgctcatgtt
    20101 gtcaggggga atcgattccc ctgtagctgg ctatctagcg cttaaacgag gggtagatat
    20161 agaggcagtt cactttgcca gcccacctta cacgagtcct ggtgctctta aaaaagccca
    20221 agatttgacc cgtaaattaa ccaaatttgg gggaaatatc cagtttatcg aagttccttt
    20281 tacagagatt caagaggaaa tcaaagccaa agcgccagaa gcctacctta tgaccttgac
    20341 gcgtcgtttt atgatgcgta ttactgaccg tattcgtgag gtacgaaatg gtttagttat
    20401 catcaatggg gaaagtctag gtcaagtagc cagccaaacc cttgaaagta tgaaggctat
    20461 caatgctgtt accaacactc ccatcattcg tcctgtggtt accatggaca agttggaaat
    20521 cattgacatc gcccaggaaa tcgatacctt tgacatttca atccaaccgt ttgaagactg
    20581 ttgtaccatt tttgcaccag atcgtccaaa aacaaatcct aaaattaaga atgcggagca
    20641 gtacgaagcg cgtatggatg ttgaaggctt ggttgagcga gcagtggctg gaatcatgat
    20701 tactgaaatc acacctcaag ccgaaaaaga tgaagttgat gacttgattg acaatctgct
    20761 ctaattcaga aaatccaaaa gaatagcgaa aatcagtaaa aaaagttagt tttttctcta
    20821 aaaacaggta aaaaactaac tttttttatt tttatgatat aatgatataa aattttgaat
    20881 atagagagtt ttctggcaat gaatcaatcc tacttttatc taaaaatgaa agaacacaaa
    20941 ctcaaggttc cttatacagg taaggagcgc cgtgtacgta ttcttcttcc taaagattat
    21001 gagaaagata cagaccgttc ctatcctgtt gtatactttc atgacgggca aaatgttttt
    21061 aatagcaaag agtctttcat tggacattca tggaagatta tcccagctat caaacgaaat
    21121 ccggatatca gtcgcatgat tgtcgttgct attgacaatg atggtatggg gcggatgaat
    21181 gagtatgcgg cttggaagtt ccaagaatct cctatcccag agcagcagtt tggtggtaag
    21241 ggtgtggagt atgctgagtt tgtcatggag gtggtcaagc cttttatcga tgagacctat
    21301 cgtacaaaag cagactgcca gcatacggct atgattggtt cctcactagg aggcaatata
    21361 cccagtttat cggtttggaa taccaagacc aaattggttg cttgggcgtt ttttcatctg
    21421 caaactggct ccaccaagaa gcctttaacc gctatatcga gtgccagaaa ctatcgcctg
    21481 accagcgcat cttcatctat gtaggaacag aagaagcaga tgatacagac aagaccttga
    21541 tggatggcaa tatcaaacaa gcctatatcg actcgtcgct ttgctattac catgatttga
    21601 tagcaggggg agtacatctg gataatcttg tgctaaaagt tcagtctggt gccatccata
    21661 gtgaaatccc ttggtcagaa aatctaccag attgtctgag attttttgca gaaaaatggt
    21721 aagttaagaa aggaaaaaac gaaatgcata ttgaacatct tagccactgg agtggtcatc
    21781 ttaaccgtga aatgtacctt aaccgttatg gacatggtgg gattccagtt gtggtctttg
    21841 cttcatcagg tggtagtcac aacgaatact atgattttgg catgattgat gcctgtgctt
    21901 cctttatcga ggaaggcctt gtccagttct ttaccctatc tagtttggat agtgagagct
    21961 ggttggttac ttggaaaaat gctcatgacc aagcggaaat gcaccgtgcc tacgaatgtt
    22021 atgtgattga ggaggccatt cttttatcaa gcacaagaca ggttggtttg atggcatgat
    22081 gacgacaggt tgctctatgg gagcctatca tgcactcaat ttcttcctcc agcatccaga
    22141 tgtctttacc aaagtgattg ctctcagtgg tgtttacgac gcacgtttct ttgtcggtga
    22201 ttactacaac gatgatgcta tttaccaaaa ctcgccagta gattatattt ggaaccaaaa
    22261 cgacggctgg tttattgacc gttaccgtca ggcagagatt gtgctgtgta cggggcttgg
    22321 agcctgggaa caagatggtt tgccatcctt ttacaagctc aaagaagcct ttgacaagaa
    22381 acaaattcca gcctggtttg ctgaatgggg acatgatgtc gcccatgact gggaatggtg
    22441 gcgtaaacaa atgccttatt tcctcggtaa tctctattta taaaaggagt tacctatgaa
    22501 ttaccttgtt atttctccct actatccaca aaactttcaa cagtttacca tcgaactagc
    22561 taataaaggc atcacagtct tgggaattgg tcaagagtct tacgagcaat tggatgagcc
    22621 cttgcgcaat agcttgaccg agtattttcg tgttgataat cttgagaaca tagatgaagt
    22681 caaacgtgca gttgcttttc tcttttataa acatggtcca attgaccgca tcgagtctca
    22741 caatgaatac tggcttgagc tagacgcaac actcagagaa caattcaatg tttttggtgc
    22801 caaaccagag gatctcaaaa agacgaaata taagtctgaa atgaagaaac ttttcaaaaa
    22861 agcaggtgtt cctgtggtac ctggagctgt tatcaagacg gaagcagatg ttgatcaagc
    22921 agtgaaagaa atcggtcttc caatgattgc caaacctgat aatggagtgg gagcagccac
    22981 aacctttaaa cttgagacag aagacgatat caatcacttc aagcaagaat gggaccattc
    23041 aaccctttat ttctttgaaa aatttgtcac ttccagcgaa atctgtacct ttgacgggct
    23101 cgtggacaag gatggaaaga ttgtcttctc aacaaccttt gactacgcct atacaccgct
    23161 tgacctcatg atttataaga tggacaattc ttattatgtg ctcaaggata tggatcctaa
    23221 actgcgcaag tatggggaag caattgtcaa agaatttggt atgaaagaac ggtttttcca
    23281 tattgagttc ttccgtgagg gggacgatta tattaccatc gagtacaata accgccctgc
    23341 aggtggtttt accattgatg tttataactt tgctcattcc ttggacctct atcgtggcta
    23401 tgcagctatt gtcgcaggag aggagttccc ggcgtcagac tttgaaactc agtattgttt
    23461 ggctacttct cgccgtgcaa atgctcacta tgtttattca gaagaggatt tgcttgccaa
    23521 atatagccag cagttcaaag ttaaaaaagt catgccagct gccttcgcgg aacttcaagg
    23581 agattacctg tatatgctga ccactccgag tcgacaagaa atggagcaga tgattgcaga
    23641 tttcggacaa cgtcaagaat aagaactatc ggattaagga aattaactcc cttaatcctt
    23701 ttgttttgtc tgataaaaaa taagagcatc ccaacaaggt agctatcata aaacttgttc
    23761 gataactatt tgaagcagga ttaggtggtc agaaattgaa ttttaatatt tcaattgagt
    23821 catagtattg tgtttgcgta tccttaaatc agctaaaagg atccatgacg acacctatac
    23881 gatatagttt tcaagatacc aaacaagtct attaatactc aatgaaaatc aaagagcaaa
    23941 ctaggaagct agccgcaggt ttctcaaaac actgttttga ggttgtggat agaactgaca
    24001 gagtcagtat catataccta cggcaaggtg aagctgacgt ggtttgaaga gattttcgaa
    24061 gagtataaaa tattcaggtg acgcatagat atagttaatt gaagctttgt ttgaaatctg
    24121 ataaaataat gatattacta agttttaaaa actaaagaaa agggaagata tgattacagg
    24181 cgaattaaaa aataaaatcg atcagctgtg ggaaattctt tggacagaag gaaacgcaaa
    24241 tcctttaaca aatattgaac agttgactta tctcttattt atgaaagatt tggatagtgt
    24301 cgagcttgga cgtgaaagtg atgctgaatt tctagggatt ccttatgagg gagtttttcc
    24361 aaaagataaa cctgaatacc gttggtcaac ttttaaaaat ataggagatg ctcaggaagt
    24421 ttatcgttta atgactcagg agatttttcc gtttattaaa aatctcaagg gggatacaga
    24481 tgatacagcc ttttcacgat atatgcgaga agctattttt caaataaata aacctgctac
    24541 gcttcaaaag gcaatttcta tcttagatgt ttttccaact aggggattag atgtagattt
    24601 tgataatgac aaacaaagta ttactgatat cggagatatc tatgaatatc tgttatcaaa
    24661 attgtcgacc gcaggtaaaa atggacagtt ccgtacacct cgtcacatca tcgatatgat
    24721 ggttgagttg atgcaaccga ctatcaaaga tatcatctca gatcccgcta tgggttctgc
    24781 tggcttctta gtatctgcta gccgttactt aaagcgtaag aaagatgaat gggaaaccaa
    24841 tacagataat atcaatcatt ttcataatca gatgtttcat ggaaatgata cggatacgac
    24901 tatgttgaga cttggggcga tgaacatgat gctacatgga gtagaaaatc cacaaatcag
    24961 ttaccttgac tcgctgtctc aagataatga agaagccgat aaatatactt tggttttagc
    25021 aaatcctcct tttaagggct cacttgacta caattcaacc tctaatgacc ttcttgcaac
    25081 cgtaaaaacc aaaaaaacag aattactctt tctttctctt ttcttgcgaa ctttaaaacc
    25141 aggtggacga gcagcagtta tcgtacctga tggtgtcctt tttggttcgt ctaaagctca
    25201 taaaggaatt cgtcaggaaa ttgtagagaa tcataagctt gatgctgtaa tctcaatgcc
    25261 tagtggtgtg ttcaagcctt atgctggagt ttcaactgct attctcatct ttacaaaaac
    25321 tggtaatggt ggtactgaca aagtctggtt ttacgatatg aaagcggatg gtttaagttt
    25381 ggatgataag cgacaaccga ttagcgacaa tgatattcca gatattatcg aacgctttca
    25441 tcatcttgaa aaagaagcag aacgtcagag aacggatcaa tctttctttg ttccagttgc
    25501 tgagataaag gaaaatgatt atgatttgtc tatcaataaa tataaagaga ttgagtatga
    25561 aaaagttgag tatgaaccaa cagaggtcat cttaaagaaa atcaatgatt tagaaaaaga
    25621 aattcaagct ggcttggctg aattagaaaa attactcaag tagggaggtg gctgtatgaa
    25681 aaaagtgaag ttgggggaag tcttatctct aaaaaaagga aagaaagcca ctgtacttgc
    25741 tgaacaaaca actctaagcc aacgttatat tcaaatagat gatttaagaa ataataataa
    25801 tttaaaattc actgaaagtt taaatatgac tgaagcactc ccagatgata ttctaatagc
    25861 atgggatggt gctaatgcag gaacagttgg ctatgggtta tcgggagctg ttggtagtac
    25921 aattacggtc ttaaaaaaga atgagcgata caaagaaaaa attatatcag attacttggg
    25981 agtctttttg gaaagtaaat cgcagtattt acgagatcat tcaacaggtg caactattcc
    26041 tcatttaaac aagaatatat tacttgattt acaattagaa ttgctaggta tcgaagaaca
    26101 agagaacatt atctgtattc ttaatacgat taaaaggctt attactaaaa gaaaatttca
    26161 gttagatgaa ctaaacttgc tcgtcaaatc ccgatttaac gagatgtttg gggaaaataa
    26221 aatatttgaa agcattgata acttatttga tattatagat ggtgataggg gcaaaaatta
    26281 tcctaaatca gatgagttgt ttagtgagga gtactgttta tttttaaata caaagaatgt
    26341 tactaaaaac ggattttcat tcgatacaaa gcaatttatc actaaaacaa aggataaatt
    26401 acttcgaaaa ggcaaacttg agcgttatga tatagtcttg acaacaagag gtactgttgg
    26461 aaatgtagcg tactacgatg aattaataaa atataaacat ttacgtataa attcaggtat
    26521 ggtaatatta cgtcccaaga caccaaatct aaatcagaaa tttattatcc atgttttaag
    26581 gaataataat tatagtcgag tgatatcagg aagtgctcag cctcagttac caattacaaa
    26641 attaaaaaaa atacttctcc ccctcccccc actagccctc caaaatgagt tcgcagactt
    26701 tgtagtccag gtcgacaaat cacaattggc aatccaaaaa tctctggaag aacttgaaac
    26761 tttgaagaaa tctctgatgc aggagtattt tggctgatat tctgccattg taattacggt
    26821 aatgatttgt tataatactt caaaggagga aatcagatgg tagtaaaaac aagaaaacaa
    26881 ggaaattcaa tcaccattac gattccaagt gaatttaata ttccaagtgg tgttaaatac
    26941 gaagcgaaat tgttaccaag tggtgagatt atctttactc ctgaagaatt ggggcagcag
    27001 gtttcttatg tatctgatga tgcctttgac ttaaatttag ataaaatatt tgacgaatac
    27061 gacgatgttt tcaaagcttt ggtggaaaaa tgacaatcta tctgacagaa aagcaaattg
    27121 aaaaaataaa tgctttagca attcaacggt attctccaaa tgagaaaatt caaacagtta
    27181 gttcttctgc cttaaatatg attgtgaact taccagaaca atttgtcttt gggaagtctc
    27241 tttatccaac aatttttgat aaagcaacga tactatttgt ccaattgata aagaagcatg
    27301 tttttgctaa tgctaataaa agaactgctt tcttcgtttt ggtcaaattt ttacaattaa
    27361 acggctatcg tttttctgta acggtagaag aagcagtaaa aatgtgtgta accatcgcag
    27421 tagaagcttt aactgatgaa aaaatgacaa gctactccaa atgggtttct gaacattctg
    27481 ttagagaaaa ggtcaaaaag taacctagta tgctggattt gaatgagcac aagaaaataa
    27541 atgaacagac aatattagaa ttctgtaatg cagaaactga tattgtctct ttttattgat
    27601 gaataagaaa gtgagaaatt atggaatcaa aagttacaat tatcatgcaa gaaatgttac
    27661 ctcttttaaa taatgaacaa ttactagcgt tgagagagag tttagaacat catctagtag
    27721 acggaaaaaa gcagcagaag tattcgaata ataacctgtt gcaactattt attaccgcca
    27781 agcaggtaga gggctgtagc tcaaaaacaa ttcgttatta ccagaggacg attgaaaact
    27841 tgtttaatgc tattaaagag tctgtgacac aactcacaac agatgattta aggagttatt
    27901 tagcaaatta ccagtctgaa aaggattgta gtaaggcaaa tttagacaat attaggcgta
    27961 tattgtcttc tttttttgct tggcttgagc aagaggaata tatcattaaa aatcccattc
    28021 gacggataaa gaaaattaag actgagcaaa atgtgaagga aacttatact gatgaacatt
    28081 tggaaattat gcgtgataac tgtgaaaatt tgagagattt ggcaataata gacctactag
    28141 catcgacagg tatgcgtgta ggggagcttg tacagttgaa tcgttcagat attgattttg
    28201 aaaacagaga gtgtgttgtc tttggtaaag gaaagaagga gagaccagta tattttgacg
    28261 ctcggacgaa aattcattta agaaattatc ttaacgacag aaaagatagt caccctgctc
    28321 tttttgtaac gctagttgga aaagcccaga gacttggaat tgctggtgta gagattcgct
    28381 taagaaagtt aggagacaaa ctcggcatac aaaaggttca cccacataag ttcagaagaa
    28441 ctttagcgac taaggcaatt gataaaggta tgcctatcga acaagtccaa aaactgctag
    28501 gtcatagcaa gattgacaca accttggcct atgccatggt caatcaaaat aatgtcaagc
    28561 attcacacca aaaattcatc tcttaaaagc aaattcccga tttaacgaga tgtttgggga
    28621 tcctttaaat aataacaaga aatttgcagt aaaaacggga caacagtgtt ttaaattttc
    28681 aagtggaaaa tttcttgata aacatgatag agtttttgaa ggttatcctg cctatggtgg
    28741 aaatggcatt gcttggaaaa gtcgaaaata tttaattgat aatcctacta tcatcattgg
    28801 tcgtgtaggt gcatattgtg gtaatgtaag aactacccac ggtaaggtat ggataagtga
    28861 caatgctata tatataaaag aatttaagaa ttccgatttc aatcttgtat ttttattaga
    28921 attaatgaaa gtaattgatt ttagtaaatt tgctgatttc tcaggtcaac caaagataac
    28981 tcaaaaacca ttagaaaatc agaaatatat cctcccccct ctagccctcc aaaatgagtt
    29041 cgcagacttt gtagccctgg tcgacaaatc acaatttgct tgtgagatag ctatataaaa
    29101 gtgtggagaa atagcttgaa atttagtata atatagctaa actatttgtt taaagtgaga
    29161 aaaaaatggg aaattttagc tttcttttaa aaaatgacga atatgaatct ttttcaaaac
    29221 cttgcattga agctgagaat atgattgcta catcaactgt ggctactgcc tttatggcgc
    29281 gtcgtgcttt agagcaggct gtccattgga tatatagtca cgattcatat ttagaagctc
    29341 cctatcgtgc tactctatct tctttagtat gggatgatga ttttagggat atcgtagatt
    29401 ctgaactcca caagcagata gttctgttga ttcggtgggg aaaccatgct gctcatggtg
    29461 gtgaaattaa ggaacgagaa gcgattttag ctttgcatca tttgtatcag tttgttaatt
    29521 ttatcgatta ttgttacagc aatgagtttg tggagcgtta ttttgatgag aagtgcttac
    29581 cactttcagc aaacatcaaa taccgagaaa ctccacaatc tatgataaag ttacaagaca
    29641 gtttaccaga actgcctgat tttcatgaac agatggctgc tcagtccgta gaagttcaag
    29701 agacttatac tgaaaaacgt gagactgcag cgcaacggca agatgtgcct ttccatattg
    29761 atcaattatc tgaggcagag acaagaaagc tctttattga tatcgatctc cgtttagcag
    29821 gatggatatt tggagaaaac tgttgtgttg agatagccgt tgatggtctc aagcacggtt
    29881 caggaattgg ttactgtgac tatgtacttt atggtaaaaa tgggaaaatt ttagcgattg
    29941 tggaggctaa aaaagcctct gtcaatccag aagtagggga agtacaggtc aaagaatatg
    30001 ctgaagcttt ggagaaacat atcggctatc agccaatttg ctttattaca aatgggttga
    30061 agcactatat acttgatggt ccgaaccgac gccagattgc aggcttttac tctcaagaag
    30121 aattgcaatt agtgatggat agacgtcatc ttcaaaaacc acttgaggat atttctagta
    30181 aaattaggga cgatatttcc gggcgtcact accaaaaaca tgccattgca agcgtttgtg
    30241 aagctttctc taatcatcgt agacaggcac ttttggttat ggcaactggg gcggggaaaa
    30301 ctcgtacagc agtttctcta gttgatatct tatcacgtca taactgggta aaaaacgttc
    30361 tcttcttagc cgatagaact tccttggtta agcaagccta tgattcgttt agaaaattac
    30421 tcccagatct ttccgtttgt aacttcttag aagataaaga aggagctcaa tcaagtcgta
    30481 tggtcttttc aacttatccg accatgattg gagcgattag tggtcaagaa gaagtaaatc
    30541 aacgcccttt tactgttggg cattttgacc ttatcataat tgacgaatct caccgttcta
    30601 tttatcagaa atacaagtcc atttttgatt attttgatgc aagaattgta ggcttaacag
    30661 ctactccgcg tcaagattta gataaaaaca cctatggatt ctttaatttg gagaatgggg
    30721 ttccaacata tgcatatgat ttggaagagg ctgttaaaga cggatattta gtagcctatc
    30781 attctatcga aaccaaactg aaactaccta cggatggtct acattatgat gatttgtccg
    30841 aagaagaaaa ggaacatttt gatagcaaat ttgaagacga tagctgtgaa aaagatattg
    30901 atgggagtgt atttaattcc tttgttttca ataaaagtac agtagaaatt gttttaaatg
    30961 aactcatgac aagaggaatt cagacagcct cgggtgatga aattggtaaa actattattt
    31021 ttgctaaaaa tcatgatcat gcagaatata tcagaggtat ttttaacaac cgctatcctg
    31081 aaaaagggag cgactatgct caggtgattg attatagtat taagcattat cagaccttga
    31141 ttgatgattt taaaattgag gagaagtatc ctcaaattgc gatttctgtc gatatgttag
    31201 atacaggtat tgatgtacca gaggttgtta atttagtctt cttcaagaaa gtacgctcta
    31261 aaactaagtt ttggcagatg attggtcgag gaacccgtct atgtaaagat ttatttggac
    31321 ctgagcagga taaggaaaac ttcttggtat ttgattatgg ggataatttt gattattttc
    31381 gtgcagatcc aagagatgga gagggtcgtc acattgtttc gttgactcag cgtttattta
    31441 atatcaaagt ggacttgatt cgagaacttc agggactcca ataccaagaa gatcagtttg
    31501 cgagagcata ccgtcagcag cttgtctcgg aacttcaagg tcgtatagag agcttaaatg
    31561 agttggactt cagggttcgt atggttttag atacagttta tagctatagg aaattggaaa
    31621 gttggcagaa tctaactgct gttacaagtg aaaccattca aaaaaatctc tctccgcttt
    31681 tatttgatga agataaagaa gatgagatgg ccaggagatt tgatttgtgg ttgcttcata
    31741 ttcagttggg gcaactgaca gctaaatctt ccactgttca tatttcccaa gtgatgaaga
    31801 cggctagagc tctttctgct attggcaata tcccgcaggt ttttgagcag gctgaaatta
    31861 tcaggaaagt acaggagcct gaattttgga aagaagttaa cttgtctgat ttggaaaaaa
    31921 ttcgtcttgc tattcgagat ttattacagt ttttggataa aacagaccgt aaaccctact
    31981 atgttaactt tgaagatcgt atactctcca ctgttcacga gaccacagca tttttgcagg
    32041 tcaacgatct tcggtcttac aatgaaaaag ttgagcatta tttgaaaact catctggatg
    32101 aggagtccat ttctaagcta taccataata aaaagttgac atctgatgat atgcttgcac
    32161 ttgaaaaatt gctttgggaa aaattaggta gtaaagcaga ctaccaaagt cattatgaaa
    32221 ataaggcaat tccgagattg gttcgtgaga ttattggctt agatagagag tctgccaatc
    32281 gtattttttc taaatttttg tcggatgaga atcttaatgc caggcagatt tcatttgtaa
    32341 aattgattgt agactacatt gtagaaaatg gttttttaga gacgaaagtg ttaacgcaag
    32401 agccgtttaa atcttatggt tctgttcaac tactcttcca acaccaacta ccagtacttc
    32461 gtaatattgt tcaaatcatt gaacttatca ataatcgagc tggagaagcg gcttaaattc
    32521 taaagtgatt gccatgctga gactcattta aaattaaaaa gagtagaaat ttatgctata
    32581 tatgagaagt tttattagga agaatgtcat cgttttccta gaatacagta tcagttgtta
    32641 agtggttgat aaatttcaaa gtagatactt gtaccacgat gtttgttgat cgagttatta
    32701 acaaaagagc tactttgatt ttaaagaaat agaaaacaaa aagccgagca agaattcaat
    32761 tgcaggagaa aatgaaataa tactcaatga aaatcaaaga gcaaactagg aaactagctg
    32821 caggctgctc aaaacactgt tttgaggttg cagataagac tgacgaagtc agctcaaaac
    32881 atggttttga ggttgtagat gaaactgacg aagtcagtaa ccatacctac ggcaaggcga
    32941 cgttgacgcg gattgaagag attttcgaag agtataaatc ttcctaggat aaagcaaaac
    33001 gcatagtatc aagggttttc aacacttgat actatgcgtt ttctgatgtt aaagactttc
    33061 taccagtttt tttaagcata attgttagtt gtagtcattt attattcttc aaagaaaaat
    33121 ggtggggcga attttttcag ttcttcaaag cacttttgag cagtatctgc atcttcacag
    33181 atgataagac agacatcatt accacaaagg gtagcgatag cgtcagggaa gctcaaagta
    33241 tcaatgatag aaccaaagga ttgagccagt ccaggaaggg tttttagtag gacttggtgt
    33301 tgaactgggc gcatccagac aagggcgtct tccatgtaga gttcgagacg tttttcccat
    33361 tttgagatgg aaccattgtt aagaacataa taagcgctat cttcttcgcg gacttttgat
    33421 aggttcatat ttttgatgtc gcgtgagagg gttgcctggg ttacttgaat gtcgttctta
    33481 gcaagaaggg cttgcaactc agcctgtgta tgaatcttgt tttttgtgat aagagcgcgt
    33541 ataagttggt ggcggtgttc tgatttattc ataataatgt aactcctttt agcaaggtaa
    33601 ggtaagcatg gactgagcga ggtcgacagt caagtggtag tctgtattgt cacggatggt
    33661 gatttcaaag tcagtagtat agaggactaa acggagagtg tctccttctt ttagcttgta
    33721 aatagttggc tgcagttcaa attgaacatc catccattca tctgcagtaa tatcctctac
    33781 taacagtaaa tcatttctat tttgtaaatt aaggtaacct tttgtcacga ctcgttgtgc
    33841 ctctggtcta aatggcaatt cacagagatt ttccaacatg tgatagcgac cgttgtcaat
    33901 ggttctagca cttaaaatag ctggataagg ttgtaggtat ttcttttgcc caaattctag
    33961 cagttgggca gataagagcc ccttgtttgt actggatttg atacgaagat tgagctgagc
    34021 gcgaccgttt aggtggagat ctttagtcac aggaaggtta atagtaatct gattggcttt
    34081 cccttgatag agctctgtat tgaaggtttg gtatgtctta ccatagcgct caaaatcctt
    34141 atctgggtac tggttttgaa tagcttgctc ttcttgacca agtgagaagg tttcacagtt
    34201 ttcttgccca ccgaagttat caagtgataa ccaagtctgt ggagctgtat tgtcctgcca
    34261 gataacagta ggaagttgaa agtctgtttc ctgtcctagt aatttcttgg tcaataaggc
    34321 atttatggac tcacggaagt caattgattg ccaattgttc atgtaaacat gggcaccatt
    34381 atggaaaaag agatgcttgt ttatatgagt aggaagagca tggaacatct ggtaaacatg
    34441 aagtggtttg acattccaat cctgagaacc atgagtaaag acaacctctg cctttacttt
    34501 atgggcattg agcagataat tgcggtcatg ccaaaactga ttgtagtccc cagtttttcg
    34561 gtctagctga gctttcactt tttctaagtc agcttggtga gcttcattgc cacggatata
    34621 gtcgccagct aagagattac gagaataggt taactcagca agggagtcaa agtcctcacc
    34681 tggataacca cctgggctag tcaccagacc gttttcacgg tagtagttgt accatgatga
    34741 aattcctgcc tcggcaatga taacttctaa accatcgact cctgtagtcg caagaccatt
    34801 ggacatggta cctagatagg aaagtcctgt tgtagcaact tttccgtttg accaatcagc
    34861 cttgacttga cgctggcgcg tgtgatcagt aaaggcacgg caacggccgt taagccaatc
    34921 gatgacattt ttataagcct cgatttgctg gtagtctcca ttagtcatga aacctgtcga
    34981 gtctttggta ccaacacctg agacatagag attggcaaag cctctcggaa ggaagtagtc
    35041 gtttagtgta tagctagagt tgatgtgagt tagcttttcc tcagcctctg ctataagctc
    35101 agctttacct tggggttgga cgagatttag ttgaggtttc tctagctcaa tcttgtgagg
    35161 aagcttaacc tcaagctcgc cctccatctt gtagagagcc ttgtcactag ccttgtcatt
    35221 ggttccctga tgataagggc tggctgtcat gatggcaggg atttttccat caaaacgagg
    35281 gcgaataatg ctaaccttta ctaggtctga tagccctttt tggtcagtat cgacacgaga
    35341 ctcaacgtaa acgacttcac gaatgacatc ctggttagaa aaagtagcca aactcttgcc
    35401 gttaaagtag tggtagtcat tatcctccgg aataagacca tcactaacaa gttggtcgat
    35461 aagagtattt ccttttttgg tgcgagtatt gagtaactga tagagatttt caatcaagtc
    35521 accatatata atgggaaatc cagtttcttt acgaaaaacg tcactatctt cgaagtcaac
    35581 caaataagaa aagcctaaaa gttgaaaagc aacagtataa aaaatatctg ctgtcagttc
    35641 atcttctgat tgaaaaaatg tcagcaggtc tgttttttta tcagctgcta ggatagaaag
    35701 tgggtagttg gtgtcttgat aagtgaaaaa gaaacgacgt aaaaaggttt caagtgagtc
    35761 tttgtgattg gctgtatttt gtaaatcaaa gccacatttt tttagttcag ataagacatt
    35821 ttcttttgga aaattgatat aactatattg attaaaacgc atagaacctc catatagaat
    35881 gacagttaag gttattatat caaaaaaaaa gcagaaaggg aattgttaac ttcaaaagga
    35941 aataatccaa taaaaatgaa taaagtacta aattcaatat agagaacaga gtaacaataa
    36001 gaataaatag atagggtata aaagttctag gagatttata ttatatgctt tctattttta
    36061 tatacaatat agtataaata taaaaatgat gacaaaaata caaatgaata gaaaataaat
    36121 tagtaagctg atgaaatttt tctcaagaga agccatttat aggtgaaaat ggtataatat
    36181 agtgagaagg atagaggaga agtgtaaatt gatcgcacaa ctagatacaa aaacagtcta
    36241 tagttttatg gaaagcgtca tttcgatcga aaagtatgtg agagcagcta aagaatacgg
    36301 ctacactcat ttggctatga tggatattga caatctttat ggcgctttcg actttctaga
    36361 gattacaaaa aaatacggca ttcatccttt gctagggctt gaaatgacag tgtttgtaga
    36421 tgatcaggaa gtgaatttgc gctttttagc tctatctagt gtgggctatc agcagttgat
    36481 gaagctttcg acagccaaga tgcaggggga gaaaacttgg tcagtcctgt cccagtacct
    36541 ggaggatatc gcggtcattg tgccttattt tgatagagtt gagtcgttag aactaggctg
    36601 tgattactat ataggggttt atccagaaac actagcaagc gaatttcatc atcctatctt
    36661 acctctttat cgggtcaacg cttttgaaag cagggataga gaagttcttc aagttttaac
    36721 agcgattaaa gaaaatctac cgctcagaga agttcccttg cgttcgagac aagatgtctt
    36781 tatatcagca agttctttag agaaactatt ccaagagtgt tttccgcaag ctttggacaa
    36841 tttagaaaag cttatttcag gcatttctta cgacttggat actagtctga aactgcctcg
    36901 ttttaatcca gctagaccag cagtagagga gttgagagag cgtgctgaac tggggcttgt
    36961 tcagaagggg ttgactagta aagaatatca agatagacta gaccaagaat tgtctgttat
    37021 tcatgatatg ggctttgatg attatttctt ggttgtttgg gatttgttgc gttttggacg
    37081 atcgaatggc tattatatgg gaatgggaag gggttctgca gtaggcagtt tggtttctta
    37141 tgccttagac atcacgggga ttgacccagt agagaaaaat ctgatttttg aacgctttct
    37201 taatcgtgaa cgctatacca tgcctgatat tgatattgat atcccagata tttatcgtcc
    37261 agattttatc agatatgttg gtaataaata tggtagtaaa catgcggcac aaatcgttac
    37321 tttttcaacc tttggagcca agcaagctct tcgagatgtc ttgaaacgct ttggtgtgcc
    37381 agagtatgaa ttatctgcaa ttactaagaa aatcagtttt cgtgacaatc ttaagtcggc
    37441 ctatgagggc aatctccagt ttcgtcagca aatcaatagt aagttagaat accaaaaagc
    37501 ttttgagatt gcttgcaaga tagagggcta tccaagacaa acctctgtcc atgcggctgg
    37561 tgttgtaatt agtgaccaag atttaaccaa ctatattcct ctaaagtatg gtgatgaaat
    37621 tccactgact cagtatgatg ctcatggagt tgaggctagc ggacttttga agatggactt
    37681 tctgggacta cgaaatttga cctttgtcca gaagatgcaa gagttgcttg ctgaaataga
    37741 aggtattcac cttaaaattg aagaaataga tttggaagac aaagaaacgt tagctttatt
    37801 tgcctctgga aatacaaaag gtatctttca atttgagcaa ccaggtgcca ttcgtctgct
    37861 taagcgtgtg caaccagtct gttttgaaga tgtcgtcgcg actacttctc taaatcgacc
    37921 gggtgctagt gactatatca ataattttgt ggcaagaaag catgggcagg aagaagtgac
    37981 tgttctggat ccagtactgg aggatatttt ggctccaacc tacggcataa tgctctatca
    38041 ggagcaggtt atgcaggttg cccagcgatt tgccggattt agtcttggga aagccgatat
    38101 tttgcgtcgg gctatgggga aaaaggatgc ctctgccatg catgagatga gggcttcctt
    38161 tattcaaggt tcattagaag ctggtcatac tgtggaaaaa gcagagcagg tctttgatgt
    38221 tatggagaag tttgcaggtt atggttttaa caggtcacac gcctatgcct actcagcctt
    38281 ggccttccag ttggcttatt ttaaaacaca ttatccagcc attttttatc aggtcatgtt
    38341 aaattcttcc aacagtgatt acttaataga tgcacttgaa gcaggttttg aagtagcctc
    38401 tctatccatc aacaccattc cctatcacga taaaattgcc aacaaggcca tctatctagg
    38461 tttgaaatcc attaaaggag tcagtaatga tttagctctc tggattattg aaaatagacc
    38521 ttattctaac attgaagatt ttatagctaa attacctgag aattatctga aacttcctct
    38581 gctagaacct ttggtaaaag ttggtctttt cgattcattt gaaaaaaatc gtcaaaaagt
    38641 atttaataat ttagctaatc tatttgaatt tgtgaaagag ttgggaagtt tgtttggaga
    38701 tgctatttat agttggcaga aatcggaaga ttggacggaa caagaaaaat tttatatgga
    38761 acaagagctt ttagggatag gtgtcagcaa acatccacta caagctattg caagtaaggc
    38821 tatttacccg attaccccaa tcggaaattt gtcagaaaat agctatgcta ttattttggt
    38881 tgaagttcag aaaataaaag tgattcgtac caaaaagggt gaaaatatgg ctttcttaca
    38941 ggtggatgac agtaagaaaa aattggatgt tacactcttc tcagatttat atcgacaggt
    39001 tggtcaggaa gtaagagaag gagcattcta ctatatcaaa gggaaagtcc aatcacgtga
    39061 cggtcgtctg caaatgattg cacaagaaat aagagaagca gttgctgaac gcttttggat
    39121 tcaggttaaa aatcatgact cggatcaaga gatgtcacgt attttagacc agtataaagg
    39181 cccaatccca gtcatcatcc ggtatgaaga ggaacagaaa accatcgttt ctccccatca
    39241 ttttgtagct aaatctgatg aattagaggc gaaattgaat ggaatagtta tgaaaacgat
    39301 ttatcgctaa aattacggaa aatagaagaa ttttcaacgt aaatgtggta taatcagtaa
    39361 gaatgttaaa agaaaaagga gcataaccaa tatgaaacgt attgctgttt tgactagtgg
    39421 tggagacgcc cctggtatga acgctgccat ccgtgcagtt gttcgtcaag caatttcaga
    39481 aggaatggaa gtgtttggta tctatgacgg atatgctggt atggttgccg gtgaaattca
    39541 tcccctagat gcagcttcag taggggacat catttctcgt ggtggtactt tccttcactc
    39601 agctcgttac ccagagttcg ctcaacttga agggcaactt aaagggattg agcaattgaa
    39661 aaaacacgga attgaaggtg tagttgttat cggtggtgac ggatcttacc acggcgctat
    39721 gcgtttgact gaacatggct tcccagctat tggtcttcca ggtacaatcg ataacgatat
    39781 cgttggtact gactttacaa tcggttttga cacagcggtt actactgcca cggacgctat
    39841 cgataagatt cgtgatacat catcaagtca ccgtcgtact tttgtaatcg aagttatggg
    39901 acgtaacgct ggtgatatcg ctctttgggc tggtattgca actggtgctg atgaaatcat
    39961 catccctgaa gcaggcttca agatggaaga tatcgtagca agcatcaaag ctggttatga
    40021 atgtggtaaa aaacacaata tcatcgtctt agctgaaggt gtgatgtcag cggctgaatt
    40081 tggtcaaaaa cttaaagaag ctggagatac aagcgacctt cgtgtaacag aacttggaca
    40141 tattcaacgt ggtggttctc caactgcgcg tgaccgtgtt ttggcgtcac gtatgggtgc
    40201 acatgctgtt aaacttctta aagaaggtat cggtggtgtt gcggttggta ttcgtaacga
    40261 aaaaatggtt gaaaatccaa ttcttggtac tgcagaagaa ggggcattgt ttagccttac
    40321 tgcagaaggt aagattgtgg ttaacaaccc acacaaagcc gatattgagc tatctagctt
    40381 gaataagagc ttgttataat ttacaattta gttaataaga ccaaaaaggt catatatata
    40441 aaggagtcac aaaaatcatg aacaaacgtg taaaaatcgt tgcaactttg ggtcctgcgg
    40501 tagaaatccg tggtggtaaa aaattcggtg aggacggata ctggggtgaa aaacttgatg
    40561 ttgaagcttc agctaaaaac attgctaaat tgattgaagc tggtgctaac acattccgat
    40621 tcaacttctc acacggcgac caccaagaac aaggtgagcg tatggcaact gttaaacttg
    40681 cggaaaaaat tgcaggtaaa aaagttggtt tccttcttga tacaaaagga cctgaaatcc
    40741 gtacagaatt gttcgaaggt gaagctaaag aatattcata caaaactggt gaaaaaattc
    40801 gtgttgcaac taaacaagga atcaaatcaa ctcgtgaagt gattgcgttg aacgttgctg
    40861 gtgctcttga tatctatgat gatgttgaag ttggtcgtca agttttggtt gacgatggta
    40921 aacttggtct tcgtgtggtt gctaaagatg atgcaactcg tgaatttgaa gttgaagttg
    40981 aaaacgatgg tatcatcgct aaacaaaaag gtgtgaacat ccctaacact aaaattcctt
    41041 tcccagctct tgctgaacgc gataacgacg atatccgttt cggtcttgaa caaggtatca
    41101 acttcatcgc aatttcattc gtacgtactg caaaagatgt gaacgaagtt cgtgcaatct
    41161 gtgaagaaac tggaaacgga catgttcaat tgttcgctaa aatcgaaaac caacaaggta
    41221 tcgataactt agacgaaatc atcgaagcag ctgatggtat tatgattgct cgtggtgata
    41281 tgggtatcga agtaccgttc gaaatggttc cagtttatca aaaaatgatt atcaagaaag
    41341 tcaatgctgc aggtaaagtt gttatcactg caacaaacat gcttgaaaca atgactgaaa
    41401 aaccacgtgc aactcgttca gaagtatcag acgtattcaa cgctgttatc gacggaactg
    41461 acgctacaat gctgtcaggc gagtctgcaa acggtaaata cccactcgag tcagtaacta
    41521 caatggctac aatcgacaag aacgctcaag ctcttcttaa tgaatacgga cgtcttgatt
    41581 cagattcatt tgagcgtaac tctaagacag aagtaatggc ttctgctgtt aaagatgcta
    41641 ctagctcaat ggatatcaaa ttggttgtaa ctcttactaa gacaggtcat actgcacgtt
    41701 tgatttctaa ataccgtcca aatgctgaca tcttagcatt gacatttgac gaattgacag
    41761 aacgtggctt gatgttgaac tggggtgtta tcccaatgtt gacagatgct ccatcttcaa
    41821 ctgacgatat gttcgaaatc gctgaacgta aagcggtaga agcaggtctc gttgagtcag
    41881 gcgatgatat cgttatcgtt gctggtgtgc cagtaggaga agctgttcgc acaaacacaa
    41941 tgcgtatccg cacagtacgt taagaaaaat ataaaaacct atcatatcca gctttctagc
    42001 ctgtatgatg ggctcttttt ttgtccaggg ggcaaaaagg gggcaaaact ttcataatgg
    42061 attgatactc ttcgaaaatc tcttcaaacc acgtcagcgt cgccttaccg tatatatgtt
    42121 actgacttcg tcagttctat ctacaacctc aaagcagtgc tttgagcaac ctgcggctag
    42181 cttcctagtt tgctctttga ttttcattga gtatgaaata agatatgcac aaattgatta
    42241 ggaaattcaa atgaatttct acaaatgttt tagcaatcgc aatctacttg tctagattcg
    42301 atctgatata ttttcgattt aatgatatgg tatttaaaac ctccaaagta gcttgctcca
    42361 ttcttttact tacgtgagtg tagatgttat ttactgtttt agcgtttttg tgttctactc
    42421 taaccattat agcattcttc tcagctagtg tactaaggag tgtgtgcctg aaaatatggg
    42481 aactaagggc tggtttatcg gtttctctag tttagtattt gccttttgca aagtgatctt
    42541 aaatgccttt ctctaaattt acatatcact attgtttaac aaaatctaat ctactttagg
    42601 tcacttattc tttttttgaa atgtagaatg aactttttca aagtttttcg aatcttttaa
    42661 aatctgtttg ttttatatcg ccattctccc ccttttttaa ttctccctat atagcctgac
    42721 aactttcccg atggtacgaa tatggttgct ttcgtctagg tggatgtcgg agtattcggg
    42781 attgagtttt tttgaggtag ccttggcgga gtttcttgac atagttagtg ccgtctactt
    42841 ggaagatgcc gatggtatta tagtcaatct gtggggtatt cttgataaat aggtagtcgc
    42901 tgtttcttat ctttggctcc atggacttgc tgacgacata agcgattggg tcgtagtcgt
    42961 ctgggataat ggaaactcca taactaaatc gtcgtcctgc atcaagcggc tacctgcaga
    43021 gataaactac ctaacacgag agtaagtagt ctgtctgtag tcgtccagtc tgatgatttt
    43081 tacgatactt cgtttttctg atcatacagt tgcctctcgg cataggtcag aactttacct
    43141 tgtctgggtg gtgcccgttg gtcgtagata gattggatat cgctaggaga atccttttga
    43201 actggaggaa agagggcatc aatcaagcta ctgaatactt taactaagtc aaatatagta
    43261 tttttcttag tagacctaac ccttttttca taatttctaa tggtgttttt acttatacct
    43321 atcttagtac ccaattctta ttgagtccaa ccattactag tctatattgt tttatagttg
    43381 attgagtttg gaatagtacg ctgtagctgc taaaacattt ctaggaatta atttgccttt
    43441 cctaatagag ttgttcatat cttatttcaa tctactatat ttttcacctc taacaatcgc
    43501 aatctcttct ttatccatga acgaaaacgc tttctatctt tgtaagtaaa gcatcacacg
    43561 aaatccacga aaatgaaaac ctttgttgtg ttttcgtaaa aaatttgttg acagagcacg
    43621 aaacgcctac tataattaaa tcaagtttaa ggaaataaca aaaaaacaaa tcggagggaa
    43681 agacgatgaa cacattaaat gagaaagtaa tcaatatctg taaagcagta gttaaagaaa
    43741 ctttaatcca agacatttag gagaaagaaa tgaagaaatc aagaaaatta gccacattag
    43801 gaatctgttc agccttgttt ttaggcttgg ctgcttgtca acaacaacat gctacttctg
    43861 aggggacgaa tcaaaggcaa agcagttcag cgaaagttcc atggaaagct tcatacacca
    43921 acctaaacaa ccaggtaagt acagaagagg tcaaatctct cttatcagct cacttggatc
    43981 caaatagtgt tgatgcattt tttaatctcg ttaatgacta taataccatt gtcggctcaa
    44041 ctggcttatc aggagatttc acttccttta ctcacaccga atacgatgtt gagaaaatca
    44101 gtcatctctg gaatcaaaag aagggcgatt tcgttgggac caactgccgt atcaatagtt
    44161 attgtctttt gaaaaattca gtcaccattc caaagcttga aaagaatgac cagttgcttt
    44221 tcctagataa tgatgcgatt gataaaggaa aggtctttga ttcacaagat aaggaagagt
    44281 ttgatattct attttcgaga gttccaactg aggcaactac agatgtcaag gttcacgctg
    44341 aaaagatgga aacattcttc tcacaatttc aattcaatga aaaagctcga atgctgtctg
    44401 tagtcttgca cgacaatctg gatggcgagt atctgtttgt aggtcacgtt ggggtcttag
    44461 tacctgctga tgacggtttc ttatttgtag agaaattgac tttcgaagaa ccctaccaag
    44521 cgattaaatt tgctagtaag gaagattgct acaagtattt gggcaccaag tatgcggatt
    44581 atacaggcga gggactggct aagcctttta tcatggataa tgataagtgg gttaaacttt
    44641 aatagtgtta tgatatgaca gtaatcttag gatttttatt tctatttttg atgacttggt
    44701 gggttatttc aatcgttaat agccagaaac cactcaaaaa tgaaacattt attggctatg
    44761 tccaaagata cgatattgat gggaatggct atcaatcatt accgtgtcct cagaactgag
    44821 aggagttctt gaaatcgtaa caccactttg aacaagagtt acttcaaccc attggctccg
    44881 ccggagtaag ttgctttcgt gaa
//
LOCUS       DAVKQB010000010        44725 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKQB010000010 DAVKQB010000000
VERSION     DAVKQB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44725
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.010"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(79..3756)
                     /gene="rpoC"
                     /locus_tag="VW528_000547"
     CDS             complement(79..3756)
                     /gene="rpoC"
                     /locus_tag="VW528_000547"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9911411.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGAIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(3789..7400)
                     /gene="rpoB"
                     /locus_tag="VW528_000548"
     CDS             complement(3789..7400)
                     /gene="rpoB"
                     /locus_tag="VW528_000548"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9911412.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            7816..7886
                     /locus_tag="VW528_000549"
     tRNA            7816..7886
                     /locus_tag="VW528_000549"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7848..7850,aa:Cys,seq:gca)
     gene            complement(8084..9415)
                     /locus_tag="VW528_000550"
     CDS             complement(8084..9415)
                     /locus_tag="VW528_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9911413.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(9644..10468)
                     /gene="endA"
                     /locus_tag="VW528_000551"
     CDS             complement(9644..10468)
                     /gene="endA"
                     /locus_tag="VW528_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9911414.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(10507..10695)
                     /locus_tag="VW528_000552"
     CDS             complement(10507..10695)
                     /locus_tag="VW528_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9911415.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(10688..11971)
                     /gene="murA"
                     /locus_tag="VW528_000553"
     CDS             complement(10688..11971)
                     /gene="murA"
                     /locus_tag="VW528_000553"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9911416.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(11995..12225)
                     /locus_tag="VW528_000554"
     CDS             complement(11995..12225)
                     /locus_tag="VW528_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9911417.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(12283..13320)
                     /locus_tag="VW528_000555"
     CDS             complement(12283..13320)
                     /locus_tag="VW528_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9911418.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(13304..13792)
                     /gene="coaD"
                     /locus_tag="VW528_000556"
     CDS             complement(13304..13792)
                     /gene="coaD"
                     /locus_tag="VW528_000556"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9911419.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(13782..14321)
                     /gene="rsmD"
                     /locus_tag="VW528_000557"
     CDS             complement(13782..14321)
                     /gene="rsmD"
                     /locus_tag="VW528_000557"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9911420.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(14386..15378)
                     /locus_tag="VW528_000558"
     CDS             complement(14386..15378)
                     /locus_tag="VW528_000558"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9911421.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTSKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(15490..16173)
                     /locus_tag="VW528_000559"
     CDS             complement(15490..16173)
                     /locus_tag="VW528_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9911422.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(16229..16969)
                     /locus_tag="VW528_000560"
     CDS             complement(16229..16969)
                     /locus_tag="VW528_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9911423.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSPALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            17003..17281
                     /locus_tag="VW528_000561"
     CDS             17003..17281
                     /locus_tag="VW528_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9911424.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            17359..18285
                     /gene="yidC"
                     /locus_tag="VW528_000562"
     CDS             17359..18285
                     /gene="yidC"
                     /locus_tag="VW528_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9911425.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVHEELAKNPSKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(18360..19154)
                     /gene="pflA"
                     /locus_tag="VW528_000563"
     CDS             complement(18360..19154)
                     /gene="pflA"
                     /locus_tag="VW528_000563"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9911426.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(19280..20530)
                     /locus_tag="VW528_000564"
     CDS             complement(19280..20530)
                     /locus_tag="VW528_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9911427.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(20597..21424)
                     /gene="purR"
                     /locus_tag="VW528_000565"
     CDS             complement(20597..21424)
                     /gene="purR"
                     /locus_tag="VW528_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9911428.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(21691..22632)
                     /locus_tag="VW528_000566"
     CDS             complement(21691..22632)
                     /locus_tag="VW528_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9911429.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(22622..23878)
                     /locus_tag="VW528_000567"
     CDS             complement(22622..23878)
                     /locus_tag="VW528_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9911430.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(23880..24542)
                     /locus_tag="VW528_000568"
     CDS             complement(23880..24542)
                     /locus_tag="VW528_000568"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9911431.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(24505..25161)
                     /locus_tag="VW528_000569"
     CDS             complement(24505..25161)
                     /locus_tag="VW528_000569"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9911432.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVANPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(25172..26050)
                     /gene="rsgA"
                     /locus_tag="VW528_000570"
     CDS             complement(25172..26050)
                     /gene="rsgA"
                     /locus_tag="VW528_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9911433.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFFNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETHKKVSKKIPK"
     gene            complement(26052..26924)
                     /gene="rsmA"
                     /locus_tag="VW528_000571"
     CDS             complement(26052..26924)
                     /gene="rsmA"
                     /locus_tag="VW528_000571"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9911434.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFACLADALKGQGL"
     gene            complement(27055..27363)
                     /locus_tag="VW528_000572"
     CDS             complement(27055..27363)
                     /locus_tag="VW528_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911435.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(27356..27997)
                     /locus_tag="VW528_000573"
     CDS             complement(27356..27997)
                     /locus_tag="VW528_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911436.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRMIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(27999..30029)
                     /locus_tag="VW528_000574"
     CDS             complement(27999..30029)
                     /locus_tag="VW528_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9911437.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREKVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSI
                     LGLGILWYQGALFMATVQLVIIALLLYGLILAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQYSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(30298..31197)
                     /locus_tag="VW528_000575"
     CDS             complement(30298..31197)
                     /locus_tag="VW528_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9911438.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(31269..31829)
                     /gene="rnmV"
                     /locus_tag="VW528_000576"
     CDS             complement(31269..31829)
                     /gene="rnmV"
                     /locus_tag="VW528_000576"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9911439.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMMVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSCKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(31829..32602)
                     /locus_tag="VW528_000577"
     CDS             complement(31829..32602)
                     /locus_tag="VW528_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9911440.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(32907..33800)
                     /locus_tag="VW528_000578"
     CDS             complement(32907..33800)
                     /locus_tag="VW528_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9911441.1"
                     /translation="MVAQDPKAQDSTKLTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(34084..34218)
                     /gene="rpmH"
                     /locus_tag="VW528_000579"
     CDS             complement(34084..34218)
                     /gene="rpmH"
                     /locus_tag="VW528_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9911442.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(34363..35577)
                     /locus_tag="VW528_000580"
     CDS             complement(34363..35577)
                     /locus_tag="VW528_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9911443.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(35733..36027)
                     /locus_tag="VW528_000581"
                     /pseudo
     CDS             complement(35733..36027)
                     /locus_tag="VW528_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36189..36641
                     /locus_tag="VW528_000582"
     CDS             36189..36641
                     /locus_tag="VW528_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9911444.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(36676..38064)
                     /locus_tag="VW528_000583"
     CDS             complement(36676..38064)
                     /locus_tag="VW528_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9911445.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            38130..39092
                     /locus_tag="VW528_000584"
     CDS             38130..39092
                     /locus_tag="VW528_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9911446.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKNYMEG"
     gene            complement(39121..39279)
                     /locus_tag="VW528_000585"
     CDS             complement(39121..39279)
                     /locus_tag="VW528_000585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911447.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHVFEVVDETDEVS
                     NHTYGKAT"
     gene            complement(39343..40353)
                     /gene="ccpA"
                     /locus_tag="VW528_000586"
     CDS             complement(39343..40353)
                     /gene="ccpA"
                     /locus_tag="VW528_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9911448.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(40778..41377)
                     /locus_tag="VW528_000587"
     CDS             complement(40778..41377)
                     /locus_tag="VW528_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9911449.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(41379..42476)
                     /locus_tag="VW528_000588"
     CDS             complement(41379..42476)
                     /locus_tag="VW528_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9911450.1"
                     /translation="MLERLKSIHYMFWVSLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGIMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VEQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     EAMTSMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(42476..43213)
                     /locus_tag="VW528_000589"
     CDS             complement(42476..43213)
                     /locus_tag="VW528_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9911451.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GEPANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(43215..44099)
                     /locus_tag="VW528_000590"
     CDS             complement(43215..44099)
                     /locus_tag="VW528_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911452.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(44086..44280)
                     /locus_tag="VW528_000591"
     CDS             complement(44086..44280)
                     /locus_tag="VW528_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911453.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIHGALGFGFM
                     IFVVFPMGMEWLKGESHDGG"
     gene            complement(44277..44483)
                     /locus_tag="VW528_000592"
     CDS             complement(44277..44483)
                     /locus_tag="VW528_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911454.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT        12748 a        10448 c         8514 g        13015 t
ORIGIN      
        1 tttaccacat ttcgtggaaa aatggagaaa gttttcagaa gagagaatga gagaaccctc
       61 gggttctctc attctctctt attctactgt ttcttccaca gtttcaacgg cagtatccac
      121 aactacttct gttgtttctt catttccttc ttcctctact ggaggattaa ggtattcttc
      181 ttcgttgaca gcatgtggtt caaggttacg gtaacgggcc ataccagtac cagctgggat
      241 gatcttaccg atgataacat tttctttaag tccaaggaga tggtctttct taccacggat
      301 agctgcgtca gtaaggacac gagttgtttc ctggaaggaa gccgctgaca agaaactgtt
      361 tgtttcaagt gaggctttgg taattcccat aaggactggg cgacctgtcg ctggaactcc
      421 acctgcgata aggacatctt tgttggcatc tgtaaagtca ttgatatcca tgagggtacc
      481 catgagaaga tctgtatcac ctggatccat gacacggact ttacggatca tttgacgaac
      541 cattacctcg atgtgtttgt caccgatttc taccccttgg ctacggtaaa ctttttgtac
      601 ttcaccgaga aggtacgttt caactgacaa gacatcacga actgcaagga gacgttttgg
      661 ttggatagaa ccttctgtca gagcagcacc acgcgctact tggcccccaa cttcgacacg
      721 catacgagct gtaaatggaa cgacatattc accttcgcca gtttcaccct taacaaagac
      781 tttcttggta cgagttgatg catcttcttc gatagcagta acttgtcctt taacctctgt
      841 aataaccgct tcccctttag gattgcgggc ttcaaagatt tcttggacac gaggaagacc
      901 ctgagtgata tcggtatttg aggcaacccc acctgtgtgg aaggtacgca ttgtaagctg
      961 tgtaccaggt tccccgatag attgggcagc gattgtacca actgcttcac caacttcaac
     1021 cgcatcacca gtcgccaagt tgataccgta acagtgacgg cagacaccgt gacgagtgtt
     1081 acatgtaaat acagaacgga tagtcacttc ttccacacca gcattgacaa tttcacgcgc
     1141 cttgtcttct gtaatcaatt catttggacc aataatcact gcaccagttt ctggatgttt
     1201 aacagttttc ttagtgtaac gaccgttgag acgctcttcg agagactcga tcatctcttt
     1261 tccttctgcg atagaacgga tcaagagacc acggtcagtt ccacagtcgt cctcacggat
     1321 gataacgtct tgggcaacgt cgaccaaacg acgagtcaag taacctgagt cggctgtctt
     1381 aagggccgta tcggtcatac ctttacgagc accgtgagtt gagaagaaca tttccaatac
     1441 cgacaaacct tcgcggaagt ttgaaaggat tggcaattcc atgatacgtc cattcggagc
     1501 agccatcaga ccacgcatac cggcaagctg tgagaagttt gagatgttac cacgggctcc
     1561 agagtccatc atcataacga ttgggttctt aggatcttga ttagcaatca agcgtttctc
     1621 aagtttttca cgggcagcac gccattcagc tgtaacagca ttgtaacgct cgtcgtctgt
     1681 gatcatacca cgacggaatt gtttggtgat ttgttcgaca cgtttgtgtg attcttcaat
     1741 gatttcagcc ttgtcatcaa cgactgggat atcggcaata cccactgtca atcctgcaag
     1801 agttgagtgg tggtaaccga ggttcttcat gcggtcaagt agggcagaag tttctgtcgt
     1861 acggaaacgt ttgaagattt cagcgatgat atttccgagg tttttcttct tgaatggagg
     1921 gttgagctca agattgctga tagcttcctt gatagctcca ccaagtggca agaagtattt
     1981 agctggaaca ccttctgtca agttggcatt gtttggttct tgcaagtatg gtagcccctc
     2041 tggcatgata tcgttgaaga gaattttacc aactgttgta agcaagacct tatgtctttg
     2101 ctcttctgtc caaggcttgt tgaggctgtc tgttgcgata ccaacacgtg agtggaggtg
     2161 aacataacca ttgcggtaag ccataaccgc ttcgtcacgg tctttgaaga ccattccttc
     2221 accttcgcga ccagcttctt ccatggtcaa gtagtagtta cccaaaacca tgtcctgaga
     2281 tggagtaact accggtttcc catctttcgg gttcaagatg tgctcagcag ctagcatgag
     2341 gatacgagct tctgcttgtg cttcttctga aagtggtacg tggatggcca tttggtcccc
     2401 gtcaaagtca gcattgtagg cttcacagac aagtgggtgc aagcgaagag ccttaccatc
     2461 aattaagact ggctcgaagg cttggatacc caaacggtga agggtcggtg cgcggttcaa
     2521 aagcactggg tgttctttaa tcacttcttc aaggatatcc cagatacgct catctccgcg
     2581 ttccaccaag cgtttagctg ctttgacgtt ttgcacgata tcacgggcaa cgatttcacg
     2641 catgacaaat ggtttaaaga gttcaatcgc catttcacgt ggcacaccac attggtacat
     2701 cttaagagtt ggaccaacgg cgataacgga acgtcctgag aagtcaacac gtttaccgag
     2761 caagttttga cggaagcgtc cttgtttacc tttaagcatg tggctcaatg atttcaatgg
     2821 acggctacct ggtcctgtga ttggacgacc acgacgacca ttgtcaatca aagcatcaac
     2881 tgcttcttga agcatacgct tctcattttg aacgatgata cctggtgcat ttaactcaag
     2941 caaacgagcc aaacggttgt tacggttgat aacacggcgg taaaggtcat tcaagtcaga
     3001 tgaggcaaaa cggccaccat ccaactgcaa cattggacga agatctggtg ggataaccgg
     3061 aaggatgtta agaatcatcc attcaggttt gtttccagac ttgtaaaagg catccaaaac
     3121 atccaaacga cggatggctt tgacacgttt ttgtccagta gctgttttca attcttcttt
     3181 gagttcagca atttcttttt caagatctac ttgcttcaaa aggtcttgga tggcttccgc
     3241 acccatcttg gcaacaaatg aaccataacc atattcacgc aagcgctctc ggtattcgcg
     3301 ctctgtcatg atagacttgt gctcaagtgg tgtatcctta ggatcaatca ccacataagc
     3361 cgcaaagtag ataacttcct cgagggcacg agggctcata tcaagggtca agcccatacg
     3421 gcttggaatc cccttgaagt accagatgtg agatacagga gctttcaatt cgatatgtcc
     3481 catacgctca cgacgaactt tcgtacgcgt tacttcaacc ccacagcggt cacaaacaat
     3541 tcctctgtaa cgaatgcgtt tgtacttacc acaagcacat tcccagtctt ttgtaggacc
     3601 aaagatcact tcatcaaaga gtccttcacg ttctggtttc aaggtacgat aattgattgt
     3661 ttcaggtttt ttgacttctc cataagacca tgaacggact ttacttggag aagctagggt
     3721 gatttgcata cttttaaaac gatttacatc aaccactatt tcttcccttt ctattctaag
     3781 tgaactgctt attcttgttc agcagcttct tctgttgctt ccgcttttgt tgctttctca
     3841 gcttcttcag cttcaaaggc tgctttagcc tcttgggctg ctttttcgcg ggctttttca
     3901 aggtcatcta cgtggatgac atcttcgtcc attccttcat ccaagtcgcg aagttccact
     3961 tcttggtcat cttcgtctag gacacgcatg tcaagaccaa gagattgcaa ttctttgaca
     4021 agaactcgga aggattctgg aacacctggt tttggaattg gtttgccttt tgtaatagct
     4081 tcataggctt tcaaacgtcc gttgatatcg tccgacttgt aagtcaagat ttcttgaagg
     4141 acatttgacg caccgtaggc ttcaagagcc caaacctcca tctcaccgaa acgttgtcca
     4201 ccaaactgag ctttacctcc gagtggttgt tgggtaacag ttgagtaagg tccgactgaa
     4261 cgcgcgtgca atttatcgtc aaccatgtgg tggagtttga tcatgtacat gactccaaca
     4321 gaaacacggt tatcaaatgg ttcacctgta cgtccatcgt aaaggattgt cttggcatcg
     4381 ctatccatac ctgcttcttt aacagttgac caaagatctt cagaacttgc tccatcaaag
     4441 actggtgtcg caatgtgaat accaagagta cgagctgcca taccaaggtg aagctccata
     4501 acctgaccga tattcatacg tgatggcacc ccaagtgggt tcaacatgat gtcgactgga
     4561 gttccgtctg gaaggtaagg catgtcttct acaggaacga tacgagagac aacccctttg
     4621 tttccatgac gtccggccat tttatctccg accttaatct tacgtttttg agcgatgtaa
     4681 acacgaacca acatgttaac acctgattgc aactcatctc catttacacg tgtaaagatc
     4741 ttaacatcac gaacgacacc atcggcaccg tgtggtacac gaagagaagt atcacgcact
     4801 tcacgagact tgtctccaaa gatagcgtgc aagagacgtt cttcagctga aagatctttc
     4861 tcacccttag gtgttacttt acctacaaga atatcacctt ctttaacctc agcaccaata
     4921 cggataatcc ccatttcgtc aaggtctttg agggcatctt caccaacgtt tggaatttcg
     4981 cgagtgattt cttcaggccc aagctttgta tcgcgcgttt ctgattcgta ttcttcaagg
     5041 tgaacagatg tgtagacatc gtccttcacc aagcgttcgc tcatgataac ggcatcctcg
     5101 aagttgtaac cttcccaagt catgtaggca acgattgggt tttgtccaag cgccatttct
     5161 ccattttcca tagaaggtcc gtcagcgatg aaatcgcctt tttcaacgac atcaccaact
     5221 tttacgagag tgcgttggtt gtaagcagta cctgagtttg aacgacggaa tttttggatg
     5281 tggtaaacat ccaatgaacc atcttcacga cgaacttcta ccttgtcagc atctgcgtaa
     5341 gtaactttac catcatactg agcaatcaca gccgcaccag aatcgtgggc tgcttggtat
     5401 tccataccag taccaacgta aggtgcctga ggattaatca atggcacagc ctgacgttgc
     5461 atattggctc ccatgagggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacctg ttttggtgaa acgtccatgt agtcaacaat attagctgga
     5581 tactcttggt tgaccccttg gtgacgtccc atgacaatct tctcagcaaa ggttccatct
     5641 tcattcagac gagagttagc ctgagctaca gtatattcat cttcttcatc agctgtcaac
     5701 caaacaattt cgttcgtgac aacacctgtt tcacggtcaa ccttacggta tggtgtttga
     5761 acaaaaccat atttgttcaa gtgtccgtaa gatgacaagt tattgatcaa accgatgtta
     5821 ggtccttcag gtgtttcgat tggacacata cgaccatagt gagtgtagtg cacgtcacgc
     5881 acttcatatc cggcacggtc acgagtcaaa ccaccaggtc ctaaggctga caaacggcgt
     5941 ttgtgagaca actcagaaag cgggttgtgt tggtccatga actgtgacaa ctgtgatgaa
     6001 ccaaagaatt ctttaactgc agctgttaca ggacggatat tgataatttg ttgtggtgtc
     6061 aagacttcat tgtcctgaac agacatacgt tcacggacat tacgttccat acgagaaagt
     6121 cccaaacgta cttggttggc aagcaattca ccaaccgcac ggatacgacg atttccaagg
     6181 tggtcgatat catctacacg gccaagtcct tcagccaagt tgaggaagta gctcatctca
     6241 gcaaggatat ctgcaggagt caccgtacga accttgtcat ctgggttagc attaccaatg
     6301 atcgttacga cgcgatctgg atcagttgga gcaacaacct tgaatttttg aagaacaaca
     6361 ggctcagtca caacggctgc atcgtttggg atgtagacaa tcttgttcaa gtcgccatcc
     6421 aaatggcttt caatgctttc aatcacgcta cgagtcataa tcgtaccagc ttctaccaag
     6481 atttctccag tttcagggtc taccaatggc tctgcaatgg tttggttgag caaacgtgtt
     6541 ttaacattga gttttttatt gattttgtaa cgaccaactg ctgccaagtc atagcgacgt
     6601 gggtcaaaga agcgagccac aagcaagcta cgtgagcttt cagctgtctt aggctcacct
     6661 ggacgaaggc gttcgtaaat ttctttcaag gcttcgtctg tacgagagtc cattggattc
     6721 ttgtggatat ctttttcaac agtgttgcga accaattcgc tgtcaccaaa gatatcaaag
     6781 atttcatcat cacctgagaa accaagagca cgaaccaagg ttgtaaatgg aatcttacga
     6841 gtacggtcga tacgagtgta ggtgatatct tttgagtcgc tttcaagttc caaccaagct
     6901 ccacggttag ggataacagt tgaaccatag cccaccttac catttttgtc tactttgtcg
     6961 ttaaagtaaa cacctggtga gcggaccaac tgagaaacga taatacgttc accaccattg
     7021 atgatgaaag tacccatttc tgtcatgatt gggaaatcac caaagaaaac ttcttgggtc
     7081 ttgatttcgc ctgtttcttt attgatcaaa cggaaggtta caaaaattgg tgctgagtag
     7141 ctagcatcgt ggatacgagc ttcttctagc gtgtattttg gttccttgat ttcatatcca
     7201 acaaattcca actccattgt gtctgtgaag tttgaaattg gcaatacatc ttcaaacact
     7261 tccttaagac cgtggtctag gaaagctttg aatgagtcag tttgaatttc aatcaaattt
     7321 ggtaagtcaa gaacttcttt gattcttgaa aaactacgac gggtacgatg tttcccgtat
     7381 tgaacgtcat gtcctgccaa gatgattctc ctttgtaaat aagttccaag ccttgtcaat
     7441 caggcttttc taatcgtcat atggttgtaa accccttatc accgtgtcct cttgacgaat
     7501 tttcagaatc tttaagcctc tgttacaaat gctcataatc ttgaaaaaaa gcacaaaaag
     7561 agcagctaaa tctgactttt tcagaagatt taactgctgt gagccttgtc tggacaatat
     7621 ttcagacaaa acctacgaca aatgattacc catattatac cctatttagc tagatttttc
     7681 aaggggtttc agtaggtttt tggtaaattt tttcccatag aaaacttggc atcacattcg
     7741 aatcacgcta tggtacaaaa aactgaaaaa actattgact gaaaatcatt ttcaaggtat
     7801 aataataaac gttaaggcgg tatagccaag tggtaaggca cggctctgca aaagcttgat
     7861 cgtcggttca aatccgtcta ccgccttcta taacttgatt tatcaggttt caaatgaaca
     7921 gaaagcccaa tttgaagggc ttttcttatt ttccctcgaa taaatacgta taactttaaa
     7981 aacttttgga gcgagtttgt ggcagagttc tttccatggc ataattccct tttgaaatca
     8041 attgaaaaaa gcttcgtcac gcctggtgac aaaagcctct agtttattct gtttcttctt
     8101 ctatttcgac ttctgtgatt tggactttta ccttggtaac acgtccattc ttcactttat
     8161 cattggttaa gataaactgt ttgttttggc tgaccaattc atagctgagt ttctcagtcg
     8221 ttggaatagt tcccactcct gtcaaataat aaccagcgat ggtatcaacg tcatcacttt
     8281 ccagttcaac atcaaagtaa ttattgaaat cattgagggt catagttcct tgaacaatgt
     8341 aggtatcctc gccaatctga tgaacatcta ttgctgcttt gtccgtttca tcatcaattt
     8401 caccaacaat ttcctctaag aggtcttcca aagtcaccaa accagccata ccaccgtatt
     8461 catccagcaa gattgccatt tgtctttggg tatttcgcag ttcttttagc aagtcatcca
     8521 caaaaatagt ttcaggtaca aaaagtggat cttgtaaaat tctcttccaa acaatattgt
     8581 caaaaccgtc cacaaagcct gccttaagga gactcttggt gtgaatgatt ccaattacat
     8641 tgtccttatc cccatcataa accgggatac gagaataatt ttgttttaaa atactttgga
     8701 taatggcttg actatcatcc tgaatatcca ccataaaggc atccgttcga ggcaccataa
     8761 cctctcgtgc catcagttca tcgagcgaaa agacaccttg tagcatctca atctcatcag
     8821 catccaatgt ttcttcacta tttgtcagca tataggcaat ttcatcacgg gtcatctttt
     8881 catccgcatc atcgaatgac ataggagtca aatggctcaa gaaattggtc gaagcagcta
     8941 aaagccaaac aaaaggactg actagttttc cgatcccaat gataatcggc gctgtacgaa
     9001 ttgccaaggc atcctttaga ttaagagcga ttctcttagg atataattcc ccaaaaacga
     9061 tggaaatata ggtcaaaaat gccaaggata gaaaagttgc cacggcttgt gctgtttcgc
     9121 cattcccaag ccaagaggca atcacacgtc ctagagtatt agttaaactc gcccctgata
     9181 agattgtaat cagggtgatt cctacctgaa tggttgataa aaagtggtta ggattttcta
     9241 gtaccttcag caggcggatg tagcgtctgt ctccttcttc cgccttttgt tcaactcggg
     9301 cacgattaag agaaaccatg gccatttctg tggctgagaa aaaggcattt aataccgtca
     9361 agataaacaa taaaacaaat tgtagcaaca aattctgact acttgggtct tccatagttc
     9421 ttctcctaaa atagtatctt atcctttatt atatcacaaa atttaatcca atacatatta
     9481 ttttttctta ccgtaacttc tcaaagtaac ttccatctct ctcccaaaac tggaagttag
     9541 ttttagaagt tgctattttt tcttagtacc tgttctagcc ctgcttaaac tagtaaaaag
     9601 aactcctaca tccataagtg acataggagt ttaggcttat cttttactga gttacagtta
     9661 cttctccagt tcggtaatcc agttgaagtc ccttttgaac attgggaact agaacattga
     9721 attccaattc tccatccgaa gacttggctt caatctgtga agctgaggga actaaatcct
     9781 cgtttgaagc gtagtaaagg gttacacggt aacggacacg cttgttttgg tccaaggctt
     9841 tacgcacctt gctttcatag tagttttgac cagtcgaata ctcggcttgt gcctgatttg
     9901 cccaggctgt ctgaacagca atgtttttag gattgcttgt tgaggcatca aaaccatcca
     9961 aaccaccgat taaggcatag cctaacaaat gacctctatc gactgcatgg gtataagagc
    10021 cctttagatt cttgacctga tgccaacctg gaggagtcca agaagttgaa ccattcccag
    10081 tttctttacg attcttgtac tgacgagtgg ccttagacaa gagggcatta gctacggttg
    10141 gaacagtttc cttgcccact gtctttgttt tattgtcagc gtagggctta cttgaaacct
    10201 tggcatctag atttgtttta ttaccattga cgataaaagc acctgagcca ttccactcca
    10261 gactcccctt tatttgactc ttgactgcgt ctgttaagac actctctgcc aatgcttgac
    10321 taggagcttc agacgcttgt tttttctgac taagattggt tttgggacta ttaggtgccg
    10381 acggcatctg cttgatataa tagctccctg tagacaaaag caataacact agcagtccga
    10441 ttagtgtctg tcttgttttt ttgttcatat ttctccttat ctttagaaaa aggctggttc
    10501 tccagcctaa tttcctgtaa atttatgaat caattcctgc cattttgctg gagacaggat
    10561 agcccatgga tcttgaccct tgcccaagat tccataacct accattaaac cgattcctag
    10621 agccagagta cctaaaatca gtactatgat gactaaaagt aaacgcttga ctacgtagct
    10681 tgatttctta ttcatcttca tcacttgcct caatccgctg aatcttagca cctagctgcg
    10741 ccaacttctc atggaaaccg tagtaacctc tatccaagtg aaccaattta ccgaccacag
    10801 tttctccctg tgctaccaaa cctgtcaaaa tcaaggctgc actggcacga aggtcagttg
    10861 aaagaacttc tgctccctgc aaaggctgtc caccaacaat acgagctgta tcacggataa
    10921 tctcagaatg caagcccatg cggcgcatct cttctaggtg ttggaaacga ttttcgaaaa
    10981 ctgtctccac catggttgat tcgccttttg caactgtcat caaggctgta aattgagcct
    11041 gcatatctgt tggaaatcct gggtggggca aggttttcac atgaacagct tttagatttt
    11101 ctagttgaga acgaacacga attccttcgt cttcttcaat tacttcaaca cccatttcaa
    11161 gtaacttggc aatcaaggga cggttgtgct cccagacagc gtctcgaatc aagacatcac
    11221 caccagtcat ggcagcagct accataaagg ttcctgcttc gatacggtct tggactacat
    11281 tgtgagtcgt accatgaagt ttctcaacac cagtaatggt tatagtctct gtaccagcac
    11341 ctttgacctt ggctcccatt tcattaagga gaatggctaa gtcaacaatc tcaggctcac
    11401 gcgcagcatt ctcaatcact gtcaccccat cagccagagt cgctgccatc atcaagttct
    11461 gcgttgcacc aacacttgga aagtccatat agatatgagc gccatgcaag cgttctgcct
    11521 tggcttcgat gtaaccagct gtctgactaa tcttaacccc catagcttcc agacctttca
    11581 aatgaagatc aataggacgg ctaccaatcg tacaaccacc tggcatggat accttggcat
    11641 gacccacacg ggcaaggatt ggccctaata caacgatgga ggcgcgcatc ttgctgacat
    11701 acttgtaagg ggcttcctca gtgatgtcgc cagtagcatc caccttgaca agatgagctt
    11761 cctcatcaaa gtcaaccttg gcattcaaac caccaactac ctgattcata ataaagacat
    11821 ccgacaaaat cggaacattc tgcaagacgg tctttccttc acttgctaga atagtcgctg
    11881 ccaacaaggg taagactgca ttttttgctc cctcgatcgt cacgcttcct accagacgat
    11941 tatcgccacc ttgaaccaca attttatcca tactcgtttc ctttactctt gatttcataa
    12001 taatcctcta agcgcttctt gccacaaatg atacaggcta ataaagaaag aactcaggat
    12061 gtagcctatg acaatgctaa aaaaggctac taataaacga acttttcttg tattatcatc
    12121 tgtcactttt aaaaccttct cccatctaac aagatccttt aaaaggtaaa aactcacata
    12181 aataaagaac atatgactac ttagagtgaa taataattga accatttgac tattatacca
    12241 tcttctctgt ttgtgcgcta gtttggaaac ttcactcaaa aattagggat tgtttttcaa
    12301 gtaatcaatc gcatcttgta gggttttaac gggcacgatt ttcatatccg tcttgattgt
    12361 tttagccgct tctagggctg tttggtagtt gtttttcgcg tccggatgcg ccttttgttc
    12421 ttcttcgcta acagggttat caggagcaaa gaaaatagca gcacctgccc tagccgaagc
    12481 tacaaccttc ttatcaatgc ctccaatgtc tcccacatta ccatcgcggt caatggtacc
    12541 tgtaccggca acaatacgac cattacgaag atctgggtga gctatttgag tatagatagc
    12601 tagactaaac atgagaccag cacttgggcc gccaatacca gctgttgaaa agctaattgg
    12661 gacattgctg attacctctg tacgatcaat caagccgatt ccaattccat ttttgccatt
    12721 ttccaaggtg ataatctttc cttctgcaga cttggtttgc ccatcctctt cataggtgac
    12781 cttgacggaa tcccctaatt tttgagaact gacgtaatca atcaagtctt tggaactatc
    12841 aaaggtctga tcattgactg ctgtgactgt atcagagata ttgagaatcc ctttaaaggt
    12901 tgaattatcc gtcacattca aaacataaac tccaaagtac ttgagttcga tatccttacc
    12961 agctgttttt agtccttgat acttggccat attttgcgat gtttgcatgt agaattgatt
    13021 gattcgcata aattcaacat cggaagaacc acctgtagtc tcctgagcac tacgaatatc
    13081 tgtaaaaggt gtcaaccaag cataaatcat atgagctaaa gtggcatgtt gaacaccaac
    13141 cgtaacgaat tgataggcac cagcttcctt atcttctgtg tcatttactt taaggacttg
    13201 gcgaatatct tccgaaccac ctggaacctc tatataataa ggcaagggca ctacaaatgc
    13261 caagaaagtc acaatcaagg ccgcaatgac gtataagggc catctaatct tttttttcat
    13321 ttcttatttc ctccaaaata ctctcgggaa catggcaggc aatatcctga ccaaacttca
    13381 aaagctctcg aacacctgat gaactgatat agagatgttc aggccggcta tgtaaataaa
    13441 tagtctctat atcagaagac agctgatgat tgtagtaatc aaaactagct tcatattgca
    13501 aatccgccgc attcctcaac ccccgtacta ggaaagtagc ccccagtctt tttgcaacat
    13561 cgaccaccaa ttcatcatga gaagccacga cttcaacatt ttccaaatgt cccaaagcct
    13621 tttctagccc ccgtttacga ttttcaagag ggagaaatcc ttgtttgtgg ggattaaaaa
    13681 aaatacccac ataaagctta tcaaaaagtc tgctcgcccg ttcaatgata tccagatgcc
    13741 catttgtcat cggatcaaat gagcctgtga ataagccaat cttatctgac atagactgtc
    13801 accttactaa ttccataaat cttttccttc cagataccca gacaggcaat ctcttctgga
    13861 agttcaacgg ccttatccgt ctcgcacaca accataacat cttcagaaaa aagctctctc
    13921 ttagccattt tttcaatatc tgctacgatt tgttccttgg catagggagg gtctaagaaa
    13981 acgaggtcaa attccccaga tacctgttcc aatgccctct ctgcatccat cttgaggagt
    14041 tgaaattttc caacttcctt ggtcatctgg atattttcag ccacgatggt ctgagcctta
    14101 cggtctcgct ccaccaaaac agcactggac atgccacgcg atactgcttc gatagataaa
    14161 ccaccactac ctgcataaag gtccaagact cgtccccctt caaagtaggg accaatcatg
    14221 ttaaaaatgg ctcccctaac cttatccgaa gtaggtcttg ttgtcttgcc ttctagtgtc
    14281 ttgaggggac gtcccccata gattcctgat acgattttca tactgtttat tataccaaat
    14341 tatagacaaa aagagaaaga aaaccgaacc ttgcggttcg attctctaca aaatattttc
    14401 gtaagtatcg cgtacttctt gaggccaaac acttgtttgc acttctccga tgtgtctctt
    14461 gcgaagtagg aacatggcca tacgagattg tccaattcct ccaccgattg tcaatgggaa
    14521 taggccattc aacaaagact tgtgccattc caattccaag cggtcttcat cacctgtaat
    14581 ttccacctga cgtctaagag tttcttcatc tacacgaatt cccatagaag acaactcaaa
    14641 ggctccacct aaagactcat tccagacaag aatatcacca tttagaccct tgtagccatt
    14701 ctcagactcg cttgtccagt catcatagtc tggtgcacgt ccatcgtgcg gtttaccatc
    14761 tggcaactcg ccaccgatac caatcaaaaa gacggctcca aattctttac aaatcgcatt
    14821 ttcacgttct ttcgatgtca agtctggata gcgttctacc aattcttctg tatggataaa
    14881 ggtgatttgt tttggcaaga tagactcgat gtcatagcga gcttcaacag ctagctcagt
    14941 caggcgaata gccttataaa tcttctcaac tgtttctttt agataaacga tgttacgctt
    15001 accatttggg ataaccttct cccagtccca ctggtcaaca taaacagagt gggttgcatc
    15061 caaggaatcc tcatctggac gaagggcttt catgtggaca aagagaccct ctccttcacc
    15121 aaagccaaaa cgagccaagg tgtggcgttt ccatttagca agtgagtgca ccacttcata
    15181 agtagcatca gggatttgga gaaccttgac cgatactgga ttttccacac cagacaggtt
    15241 gtcctgcatt ccgtcaccga ccttactcaa gataggacct tgaacttcga caacttctag
    15301 cttatctttc aaatactggg taaaagtgtt tttgacaaag gaaatttctt cttgttgatg
    15361 gataaaactt ttcttcatag acaactcctc aaaaatttaa taaacgtatt atacacctat
    15421 ttccaagaaa agaaaagaga aaatttaaaa aaaatcagtg tcactcaaaa cactgatttc
    15481 atagaccttt tagtcattgc gaccaaagat acgtagaata cttaggaaaa gattgataaa
    15541 atcaagatag atgctgagag ccattgacac cacccagcct gtcgctacac gaccttgtga
    15601 ttgttcatag gcgaggcgaa tcttttggtt atcccaagca atcaaccctg aaaaaaccaa
    15661 aaccatggct acactaatca tataatcaaa gaagccacta gccaagaaaa tattgactac
    15721 catagcaatc agtaaaccaa tgagagctgc catcattgct cgaccaatcc cactcaagtc
    15781 tttcttggta aagataccaa ctgccgccat gacaaagaag agaagggcgc tcgatacaaa
    15841 ggcagataaa actgtacccg gagtatagaa agccaccaca aaactaaggg taaagccgtt
    15901 taaaacggag taaagtaaaa atactggaag agcagctgga ctatttcttg aagccatgct
    15961 actggcaacg aaaaccaaag ccaactccgc aaaagtagca atggtcaacc agagacgccc
    16021 ctgcatcaac aagtaaacca actgagactg aaagaccgtc aacataaggc ctgataccaa
    16081 agcagatagt ccgattccca gaccaacaaa ggcataaacc ttagcgtaaa attgattgag
    16141 acctgcacgg tcatgtataa tagtgtgatt catccttttc tccttttctg tgaacatctt
    16201 tccttgatta taacaaaaaa agttaaaatt agcttaaatg gaagattaaa ataccggctg
    16261 caactgcaac attcaaactc tcggcctgcc ccttcatgct aatatggacc aactggtctg
    16321 cactttcagc cataagggga ctaattcctt gaccctcatt tcccataact agtacaaaat
    16381 tttctataga aggcagttct ctgtaatcaa cagaatcttt agatagggtt gttgctagca
    16441 ctgggatagc taccttttta gtttcgtcaa gaagcgctgg actagtcatc cggtaaatgg
    16501 gcagatggaa atgactacct tgcatggaac gtagggtttt gagactgtag atgtctgccg
    16561 acttatctga aacaatcact cctgtaaaac ctgctgcatc cgcagttcga atgatagttc
    16621 ctacattacc gggatcttgc acatcttcca aaaacaagaa cttgccctga ctcaaatcag
    16681 cttgtcctac ttcttctttt tgaaccaccg ccacaattcc ctgtggagtc tgagaatccg
    16741 ccaaatctag caaaatatcc tctgaaatcc agacagtttg cggaaaagca gctaactgat
    16801 ctcgataatt ttctagggca aagattttct caatcgtcac tccagcttgg acagcttctt
    16861 caaacaagtg ccagccttca atcaaatagg cagacttgcg gtattttttt tggtgtaatt
    16921 tcttggcatt ttttaccaca gaattggctt ttgaggttat aatagtcata gaaatattat
    16981 aacacaatca aaggggtttg gtatgcaaaa ggttagaatg attgcccaag gtagggtaca
    17041 gggagtcggc tttcgttggg gtgtttacag cttggcactt gaaattggtg gcatcacagg
    17101 tcgagtatgg aataacgacg atggcacagt ggaaatctta gcccaagcag actcatctgc
    17161 tatcatggca aaatttatcc aagaaatccg aaaaggaccg acaccttttt caaaagtaag
    17221 ctacttagat gtcaaactaa gcaactttcc tccctactct gactttaaaa tcgcaaatta
    17281 ggtctccaga actattgtat attttgtaaa aaaacagtag aatagaaagg tataattttt
    17341 aaagaaggaa taaaaacagt gaaatctatt aaacgttttg cactctcagc tatgggagcg
    17401 gctatgttgc ttgtcttgac tggttgtgtc aatgtcgata aaaccacagg tcagccaaca
    17461 ggatttattt ggaataccat cggagcccct atggctgaag ctatcaagta cttcgctact
    17521 gataaaggtc taggctttgg tgtcgctatc atcatcgtaa ccattatcgt gcgcttgatt
    17581 atcttgccac ttggtatcta ccaatcatgg aaggcaacgc ttcactctga aaagatgaac
    17641 gccctcaagc acgtccttga gccacaccaa acgcgtctca aagaagcgac tactcaagaa
    17701 gaaaaactcg aagcccaaca agctctcttt gctgctcaaa aagagcacgg tatcagcatg
    17761 tttggcggtg taggatgttt ccctatcctc cttcaaatgc ctttcttctc tgctatctac
    17821 tttgctgccc aacatactga aggggttgct caagcaagct acctaggcat tcctctaggt
    17881 tctccaagta tgattttggt tgcctgtgct ggtgtccttt actatcttca atcgctcctt
    17941 tcacttcacg gagtagaaga tgaaatgcaa agagaacaaa tcaagaaaat gatttacatg
    18001 agcccactca tgatcgtcgt cttctccctc ttctcaccag ctagtgtcac actttactgg
    18061 gttgtcggtg gtttcatgat gattctccag caatttatcg tcaactatat cgttcgtcca
    18121 aaacttcgca aaaaagtcca tgaagaactg gcaaagaacc catcaaaagc aagtgctttc
    18181 tcaacaccaa gtggacgaaa agacgttacc cctgaacaac caactgctat tacaagcaag
    18241 aaaaaacaca aaaatcgcaa cgctggaaaa caacgttcga gataagaaga aaaaggctta
    18301 gctgatttgc taagtctttt tgcaagtcaa aagcccgatg tttccatcag gcttcttttc
    18361 tatccatgta cacgtttcat ataatcttga taactttcgg tatccatgag ttttttagcg
    18421 ttcttgacgc gatctgctgt tggtggtttg actccttcga gggaatatgg aattccaagt
    18481 tcacgccact tgaactcacc catggtgtga taaggtagaa tttcaaactt atcaacattt
    18541 ttgagggtct tgacgaactt accaagttca atcaagtcat catctctgtc tgtcaatcct
    18601 ggaactagca cgtggcgaat ccagacaggt tttccaatat ctgatagata ctgggcacaa
    18661 gccaagatat ttttattggt ttggctagtg acaatcttgt gctgttcttc gttgatttcc
    18721 ttgatatcca aaagaaccaa gtcagtgaca gccatgagtt tgtcaaactt ctcaaggtaa
    18781 cgcggtttat tacggaaagg aagagcacag gtgtccaagg tacagtggat tccttgttcc
    18841 ttagccttgg tgaagagagc aatcaggaaa tcaatctgca agagagcttc tcctccactg
    18901 actgtaatcc cacccttatt tccccagaaa ccacggtagc gcaaggcctc tgtcaagaca
    18961 tcatctaccg tccgttcacg tgacttattg gactccatag cccaagtgtc tgggttgtgg
    19021 caatactggc aacgcatgtg acagccctgc aaaaagacaa taaagcgaat accaggccca
    19081 tctactgacc caaagctttc tgtcgaatgc accatccctg tcacttgtcc ataatcaatt
    19141 gtttcttcag acatatcgtt accttccttg aaaacgtttt atagttttat tatatcacga
    19201 cttgaaggaa aaacaagatt tttgcctatt ttttagaaag caatctgttt ttcaatttca
    19261 ttttcaatct attatcagat tattcttcaa aatcgaagcc atataaggtt gcaaaatagt
    19321 cctcagggcg ctctgcacgg cggatttgac gggctttacc ttcttcggta tagaggattt
    19381 ccgcagaacg taatttggca ttatactggt agcccattga aaatccgtgg gcacctgtat
    19441 catgaattac cagcaaatca ccgatttctg tatgaggcag ttcgcgattg actgcaaatt
    19501 tatcattgtt ttcacagagt gaaccaacca catctaccgc ttcagctggt ccatctggat
    19561 gggtcacgtt gctaatatga tggtaagctc cgtacatagc tggacgcatg aggttgactg
    19621 ctgaggcatc tacacctaga taggtacggt aggtttcctt cttatgagtg actcttgtga
    19681 ctagagcacc gtgaggtgcc agcataaaac gacccaattc ggtgaaaatc ttgacctgac
    19741 caagacctgc tgacgtaaga acttcttcat acaccttacg aactccctca ccaatcaagg
    19801 cgatatcgtt cggctcctgg tctggacgat aattaacacc aataccgcca gaaagattga
    19861 taaagtctaa cgaaatgccc aacttttcct tgatttcaac agccagttca aagagctgac
    19921 gagccaactc tggataatag agatgggtca cggtattgga cgctaggaag gagtgaagcc
    19981 caaaagtctt ggctcctttt tccttcaaga tagcaaaggc ttcaaagagc tggtccttgg
    20041 tcatgccaaa cttagcctcc ccaggattgt ccataatgtc tgtccccagt tcaaaaacgc
    20101 ctccaggatt ataacgacaa gagatgattt ctggaatgcc tgctactctc tccagatgtt
    20161 caatatcttc aaaggcatcc aagttaatgg tcgcacccaa ttcacgtgca taggcgtatt
    20221 ccttgtctgg cgtgttgttg gaagagaaca taatctcaga acccagaaag tccagtttat
    20281 ggctcatcaa aagctctaca taactagagc agtccacacc acaaccttct tcttggagaa
    20341 ttttcaaaat agctggagtt ggagtagcct taactgcaaa atattcctta aagcccttgt
    20401 tccacgaaaa agcttggttg acagctcttg ccttctcacg aatccccttc tcatcataca
    20461 agtgaaaggg agtcgggaac tcggcaacaa tcgcttctaa ctcttctcta ttgataaatg
    20521 gtgttttcat aagcttcctt ctattctgtt tgcaaagaaa ggctgggaca atcgttccaa
    20581 cctttagttc tatctcttat ttatcttcgt caaagatatt gccaacctca acatcgatgg
    20641 cttggttctt gacatcaata ttggttacct tcaagagtga cttgtagtca aactgctttt
    20701 cacgttcttc ttgggcattg tccgcaaaga ccgctacacc tgccagttct gagtcgaact
    20761 cgcgcaagag actaatcata ccgttgaccg ttccgccacc tttcaagaag tcatccacaa
    20821 tcaagacacg gctgcctgcc ttaagactac gttttgaaag gaacattttc tcgatacggt
    20881 caccacttga acctgaaaca tagttgacgc taacagttga accttcggta attttcaggt
    20941 cacggcgcac aatgacaaaa gagacattga ggacattggc aactgcattt gcaagtggca
    21001 cacccttagt tgctacggtc ataaccgcgt caattttttg gtccataaag cttttggcaa
    21061 taatacgacc aatatttttc aagatggctg gtgtgctaag caaatcagac agatagatat
    21121 aaccacctgg caagatacgg tcactttctg acaacttggt acgcaagtct tcaaccattt
    21181 ccttagcatc ctgactcgaa atagacggtg tgaagatgac acctccgcca gccccagtca
    21241 ctgtctggat atgaccgatt tcaatttcct caaaggcgcg tttgataatg acgatatctt
    21301 ctgagatgga tgatttagca gactcatact tttcagcaaa agtattgaga ctagttagtt
    21361 tataaggatt attaatcaaa tagttggaaa tgacaaccat ccgatcactt cttcttaatt
    21421 tcatttctat ttcctattcc atttttattc aaaaaatcaa aaagcaaact agaaagctgg
    21481 tcgctggtgg ttcaaaacac tgctttgaga ttgtcgatag aactgacaaa ccctgtaata
    21541 tacctgcata tatacatacg acaaggcgat actaccctag tttgaagaga ttttcgaaga
    21601 gtattcattt ttgtctttta cttattatac catattcaca taaaaaaacg aacattctta
    21661 tcctaaaaaa tgctcatttt tcttaaatta ttaatctaaa tctggtttat agaaggaacg
    21721 attatccata gcgaagattt tattggacat ctctccttta tccaccaaag ccagagctgt
    21781 tgacatcatc atcatgcttg catccagatt gtcaatcata tggataatct ctgcctccat
    21841 aatacgtgga cggactggac ttccatattc aagcaagccg tggtgactga ggatgacatg
    21901 acgaagcaaa acgacttctt ccttggtatc gtcgatgccg agttccataa ctgtcttggt
    21961 aatttcgcta tcaatgagag cgatatgtcc aagaagattg cctcgcactg tgtactctgt
    22021 ctggtctggc cccgtcaact cgatgacctt agctaagtca tgcaacataa tccccgcata
    22081 gagcaggctc ttattgagct gaggataaac ttcgctaata gcgtctgcca aacgcaccat
    22141 ggtcgccgta tgataggcca agcccgtttc aaaggcatgg tggttggtct tggcagctgg
    22201 ataggagtag aattccttat catacttggt gtagagattt cggacaatcc gttgccagac
    22261 aggattttca attttgaaaa tcatttgcga catgtagtca cgaatttcct tgacatcaac
    22321 tggtgacttg accttgaaat cagctgggtc attgggttca ccagcttgag gcaggcggag
    22381 agtaatttga ttgacttgag gggtattgtt ataaacttct cggcgtcctt tcatgtggac
    22441 aaccttacct gcggtaaagg cctcaatgtt atgaggttgg gcatcccaga gcttcccatc
    22501 aatctcgcca ctatcatctt ggaaggtaaa ggctaggtag tttttcccag ctcgagtttg
    22561 cctcaggtca gctgatttga ttaggtaaaa gccttcaaat aactcatctt ttttcatgtg
    22621 actaatcttc atattcttcc tcattttctt gaaaatggag tagatcaagc gcaggctcac
    22681 cttctgacaa ctcaatgtga cggagcgtcc gctcgatagc tatggtacga cggtttaata
    22741 attcatcaat attgccagag gcatgttgga gatgtttttg tgccttgacc agaataccac
    22801 caaacttgcc aaactcggtc ttgacactgg caagagtctt gctgatatgg tcggcactct
    22861 tttggatatt aagggtcttg aaaccaactg atagggagtt aagaagggct gatagggtac
    22921 ttggtcctgc aacaataatc tgttcttccc gtctcaaatc atcaaagaag accggattgc
    22981 ggacgatttc tgagtagaga ccttctgtcg gaacaaacaa gattccaaaa ttggttgtcc
    23041 gaggcggtgc tatgtacttg ctcttgatat ccttggcaaa gcgcttgacg cttgctagga
    23101 gtgacttacg acagcgttca atctcatcct tgtcacctgt ctcataggct tcttccaagc
    23161 ggtaataatc tgccagtgga aacttagagt caattggaag atagacgtat tcttggtcgc
    23221 cttgtccggg taacttgatg gcatactcca ctcgttcact agagttttca accgttgcgt
    23281 attctcgttc gtactgggca ggtgtcatga tgtcttcaat aatttgcccc agttgcaatt
    23341 ctcccagaat ccctcgcgtc ttggttccag agagaacctt gttaagagct ccgacatcac
    23401 gggcaactgt ctgcatttct ccaagaccac gattgacaga ctccagttgt ttagaaactg
    23461 tctcaaagga agcctgtaag cgtgtctgca aggtcttttc tagtttttcc tcgaccgtct
    23521 ggcgcatttg ttccaaacgt tgctcatttg attcctgcaa ggcttggaga cgttggtctg
    23581 tcttgtctct agtttggagg agattatctg tcatttcttg acggacttgg gtcaggcctt
    23641 ggtgcaattc aatccgcact tcttgcaaac ggtcgctgac agccacttcc aaatcttttt
    23701 ggtctaactg gctggcttgt ctggcttggt caaagcggta atccaactgg tctgacaaat
    23761 gatctgcctg atcctccaag ctcttactta agtgtttctc ctgcctatcc tgcctttgcc
    23821 aaatcagaaa gagaccagct aggttggcaa ttaataatag aataagtaaa ctttccatcc
    23881 tacctcctat ccttgctatg cagtacgacc acataaccat ctgggcaagt taccgacact
    23941 tccctatcta tatattcgtt agaagcgtac acttttttaa agaaaaaatt ttcctctgtc
    24001 aactcatact tggctccaag aatagtcagc tggctatccc gaactggcat aaaggctaga
    24061 tagtcgtagt ctgaacgagg ttctagctga ctggtccctt ctggacaata agtaatcaag
    24121 ttttgcccat cctcaatttc tatttgatgc atatagggtg ccaacttagg attgctaggc
    24181 agaaagacat tggccaacat atggtcaata cggccaccca aggcaccgaa aatagtaacc
    24241 tgagcctgag gattttgttc aaagatggtt aagagagcca attccaaatc ggtatcatct
    24301 ttttctggtc gtgcttggac aaaatgctgg gcacgttttt gaatcacctg tcgctcttct
    24361 tcggtcacag aatcaaaatc cccaactgct agagcaagag gcaagtcttc ttccaagacc
    24421 cagagcgagc ctcgatccac cccaacaaaa gcatcaaaat ctgtccgata atgaccgcgg
    24481 tctccgcctg caaaaacggc aaccctagtc cagttgtttt ctgagagttt gtactcgctc
    24541 attgacatct cccttaaaga cataggaacc tgctacaaaa acggtcgcac cagcttcttt
    24601 agcttgggca atagtttggt catcaatccc accatccact tcgatttcaa agttcaaacc
    24661 tttttcctca cgaagagcaa ccaactcacg aaccttatcc atggtttctg gcagaaaggc
    24721 ttgcccacca aaacctggat taaccgtcat gactaaaact tggtcaacta gatgaaggac
    24781 gtgcttgatg gcttcaactg atgtgccagg attgataacg actgaaggct taactccgag
    24841 tgaacgaatt ttttggaggg cgccatgaat atgaggcgtt gcttctacat ggatactgat
    24901 gatgtctgca cctgcgcgcg caaagtcttc cagatggtgt tcaggattcg ccaccatcaa
    24961 gtgacagtca aagaccatct tactatgtgg acgaagtgct tcaaccacac cggcaccaaa
    25021 actgatttgt ggcacaaaat gactgtccat gatatcgata tgggcatatt ctgccccagt
    25081 tgcttctaga cgtttgattt cacgttcaaa gttggcataa tctgctgcca gaattgacgg
    25141 agcaatcttg tattgagaca taggtttctc cttattttgg aatttttttg ctgacttttt
    25201 tatgggtttc tctacgattt tcaatttcac taaggaattg caggtaattg tcaaaacgga
    25261 aggttgcaat aacaccctct tcaacagctg gtttgacggc acaagacggc tcatgggtat
    25321 gggtacaagt acgaaactta caatctcggc tgacagtagc aatctctggg aaagcctgat
    25381 tgaggtcttc agcccttgat acttcatagt ccagagatga aaagcctggt gtgtctgcga
    25441 ttttaccccc attgaggttg aaaaaactaa cagctcgagt agtatggcgc ccacgaccca
    25501 gactgtctga gatttctcct gtttcaagat tgaggtctgg tgcgattttg ttgagaagag
    25561 ttgacttccc aacacctgtc tgccccataa agaccgtaac cttgcctgtt aacaaagaca
    25621 ggagttcctc tttactggtc acaaagtcat agccgatgtc accataggtc tgctggtaaa
    25681 aatccagttc tcccctatct tccaacaaat ccattttgga aatatagaca atgggatgga
    25741 tgcccttgtg ctccaaaaga accaagaaac gatccagcaa attgctgtta aaatcaggtt
    25801 ccttgacgga catgattact acagcttgat cgatattgac aataggcgga cgaaccagac
    25861 tgtttttccg ttcgtgaatt tttaggatat agccttctga attttcctcg gcagagaaat
    25921 ctacccagtc cccaacataa ggggtatggc cttttttacg gaaattccca cgcgcgcgtg
    25981 tttgataaac ctggccatca ctctccacat agtagaaacc tgccaaggct ttaatgattt
    26041 gtccctgcat cttagagtcc ttgcccttta agtgcgtctg ctagacaggc aaattctgcc
    26101 aagctgagag cttccccacg cacacttggt gacaagcctg cctggtccaa agccttggtc
    26161 agcttgtcct tgacctcttc agtcttacca aagtaacctg tcaagttatt ccacaaggtc
    26221 ttacggcgat gggtaaaact agccttggaa accttaaaga agaagttctc atcttccact
    26281 gctacagctg gctctggacg gcgcaccatt ttcaagatgg ctgaatccac atttggcgct
    26341 ggcacaaaga ccgtacgagg cacgataaag gcaaccttgg ctgtcatgta atactgcacg
    26401 gcgatagaca agctaccgta agccttggtg ttaggctggg ctgaaatgcg gtccgctact
    26461 tctttctgca tcatgaccac aaactcacta aaaggaatcc cactctcaat caagtgcatg
    26521 agaataggcg tcgtgatgta gtaaggcaaa ttagccacta ccttgattgg caggttagga
    26581 tttttaaaat tttggatatg ttgcgccaaa tcaaccttga gaatatcttc gttaactaca
    26641 gtcacattat caaaatcacg cagggtatct gccaaaattg gcaccaaacg gtggtcaatc
    26701 tcaaaagcca tgacttgggc tgcacgctca gccagaaact ccgtcaaggc accaatacct
    26761 ggcccgattt cgatgacatt gacctggtca tcaatttcag ccgtatccac aattttttga
    26821 aggatattgg tatccgtcag gaaattttgc ccgaaggact ttttaaaggt aaaaccgtga
    26881 cgctccagca ctgccttggt cacgctataa tctgcaattc tcatctattc tcttttctat
    26941 tatctgttac tactattgta acacattccc cttgtatttt gggaatgaaa atcataccaa
    27001 aaaactatct ttcctcatga agaaagatag tcttggaaat ctttaaaagt ggccttaaca
    27061 gttctctcga aacatgcgtt caaaaaaatc agcttcttga taacttcgat tttcgggtag
    27121 ggtcaaatcc acatcctcct ccacatcatc cactgtcagt tcaaccgtct gaggtaaatt
    27181 tttcaaacga taatgctttt gcctttgccc ttcaatacta gcaaaatcaa gcaaatagcc
    27241 atgtactcgg atttgaacca aaaggagatg attggtcata ttttccatga tgaggatatc
    27301 ttctaaacca cctctggcac taggataatc agatactctg cgaatacgga ttttttcaca
    27361 catgagcaag tttcctcata tcaatgattt catcagcctt attccagact aggggattat
    27421 gggtcgcaat gatgatcatt cgattttcat ctttcaaatc caccaagaga ttcataacct
    27481 cctctgaatt ttcaggatca agagctgctg ttggttcatc tgccaaaatc aagggtggat
    27541 ttttcaaaat agtcttagca agggcaactc gttgggcctc tcccccagat aaagtataaa
    27601 ttttttgttc taaatccaaa taccctagat taactttttc taaagccccc acttgccttt
    27661 ccaaacgttc tacttttgag attttctgac caacaaaacc caaatccaaa ttttctttga
    27721 ttgattggtt ttctaagagg ccaaaatttt gaaagagata gcccatctga tctcgaaaat
    27781 actcacgagt gggaatggtt tttaaatctt tcccctgata gagaaccctt cctccatcta
    27841 tcttttctag ctttcccaag atattcagca gcgtcgtctt tccgcttcca ctctttccga
    27901 ttaaggcata aaccttgccc ttctccagct tgagattcaa accagagaaa atcgcgcggt
    27961 cattaaattt cttttccaat ccttgaatat caatcatctt attttccttt caatactgcc
    28021 atgctaccag cttcttcttt tttgtcctgc cttactaaaa tcaagagggc gttaagcgta
    28081 aacaaagcta caactagagc gctaatcaaa ccatctcttg tcaaaatact agataggact
    28141 aggccaagca agagaactcc tccttgcgcc agtaaatact tgccatgaag ctcatagatt
    28201 gtcataccag caagacgttt aatcataagc tcccgtctga actgctcaaa atagagaaga
    28261 ttcatggaat caaataacaa gatagccgta gacagggtca aaatcgtccc aataaggaga
    28321 gagtaaaact ccacccgttt gtcatttagt accttggcaa aaaatagctg gctttttacc
    28381 aagtaagaaa ccttgtgata cagaccttgc tcttttagag ctgtgatgct gtctccatat
    28441 cgatctagtt tcaaggcact attagccgta gttccccaca acatccctgc aacaggtctt
    28501 gttccagtcg cttgcggcgt tagaactaca ataatcggat cttttaggta ctggcgtgtt
    28561 gtcttgtacc catcattata gaggaaacgt tcctgtcctg tttctgtaaa agctagcctt
    28621 acctgtggga agtagtgttt ctgactcgtc acttctacac tttcagatga aaagttttgc
    28681 aggtaatctg taaacaatcc ttggtagtag gcagactgct ctcgcaagct ctcaggcaag
    28741 atcagcccaa actctccctc agacaagttt tgcatcttgt ccataaactc tgaagaaacg
    28801 gtaatctttt cttctatcag atagcgtggt gagacataga taacattccc tgacggtgtg
    28861 tagtcactga gacgattacc atctaggtcc acttctgctc catctgagaa attgtcaaca
    28921 ttgctcataa tataaaaaga gtctgtattg gctaaccgtt cttcagtaaa tgtctgccac
    28981 tcacgattat ccttacgcgt tccttcttca tcggcaaatg cactagacca accaaaggat
    29041 agacggtaac ggtctgagta ctggctccat ttattgctag ctctctccat ttcctgcatt
    29101 tcacggtagt ggggtaggag agctgtcgca ctcgatccga ccaccaatac agctaagagt
    29161 tgccccacca tcatcaatgt catcatacgt ttgagaggga gtttcccttt caatagatcc
    29221 accagactat tttcctgtaa accaagtaga tagacgacac ttagtaaggt agaaatccct
    29281 gccaagatca atccataaag tagaagagca atgatgacca gttgcaccgt tgccataaac
    29341 aaggcacctt gataccagag aatccccaat cccaaaatac tggataccag cactgagcag
    29401 ataagctgtc tcacatcttc taacactggt ctgagagcaa ctccaaacaa gctctcacca
    29461 gctattaagc gaatccctgc ctgacgaagg gatttgatcc gataaatcag ggtcagactc
    29521 ataaaggtca gcagaaaaat agctaaactc agtagcacca taggggtggc cgtcaatagt
    29581 aagactatcg aaaatggatc ttctccattc gaaacaaagc cttgatagcc aagttctttc
    29641 aaggtggtct gtaagctcac tccatccaaa cttcctgaca caatcaagta agaccctaat
    29701 aaatcactcg tttctgcact ctccttggag gaaatggtca gaccttctgg aagctctccc
    29761 tcaccataag tggcataggt aaagagtgtt tctccagctt catttggctc aacgattcga
    29821 cgggcgatga gactattatg ctctaccgcc aagtgactca aagcctctgt caccttttcg
    29881 cgactagcta ctggttgatt tatcatcatt cctgtcacat caacagaagg atagttacca
    29941 aataaaatct cttctcgatg gtcaacggaa gttatcacca tataaagcga taccaatacc
    30001 attgaaatca aaataaataa acgtttcatc atttcctcca aaaagttact tggtcactta
    30061 gtgagaaggt gaccacagtc actgcgatgt taagaagctt attaagttct ttctgctagc
    30121 aacatcttca aatatattat atccgttttt tcaagaacta tcaaggacat caatgtcctt
    30181 aaaatctaac tatttcatat tatttttagc ttatatctaa aaaaacagtt ctaaagacga
    30241 actttagaac tgttttgata cgaggacaag gacatttttg tcactgattc actatgttta
    30301 taaatatttt ttgacatcct cttgccattc ctctgtcaat ttctttacta gaaatccatc
    30361 tccaattagc ttcgcaaatg ttttagaaga ctgataaaat gattcagcta aactaaaatc
    30421 tctttctact cttaatgctt gcttccacaa aaacataaat acaagaggtt tcttttgata
    30481 atcttgagtt ttatccataa tttcttgtaa acacgataga taatcattaa aacaatcagt
    30541 tactcctacc ttttcaagac aacacattcc tgaaataaga gtatctctaa ttataaagta
    30601 attctctatt tgagttacct tcttctgttc caaaagaaag agcattagct tttcgatttg
    30661 atttataaat ttggcaagat cttttctaat gagcatttga taaaaaaaca aacgaatcaa
    30721 caacatatca tttgttgaat aaacttcttt ttctatgatc tgagacatat gttcctcaag
    30781 tattaactct gcatattcag gccttctagt tctagaagta tccaaacacg cctgcaatac
    30841 ttcacatgct aattgttctt ctttaggaag attatcaaag tatgttttat aaatctcttc
    30901 caaacacgct tccttcttat cgtactcctc ttctttacca tagattggtt cacgtaaaat
    30961 ctggtatttc aattctaaat aagcagaagg aagagctgaa aaatccggca taaggctata
    31021 aactggaact cctagccggc gagcaatata gtctaacttg ggcaaactcg gttgcgaagc
    31081 tccactttca attcgcgaca gttgacgaac tgtcagttct tgctcatctc cacaaaaatc
    31141 tggacgacta attccttttt ctatccgaat ctctttaatt ttttgaccaa ttaacatcag
    31201 acgcctcctc ttctacctgc ttcgaaaaat actgtgaaac ttaaaaaatt gtaacggtca
    31261 catctttcct actcataaga tttcatagct tcttccactt ctgccaaagt aaccccaaac
    31321 aactctaggc gtttgaggag ttgcttgccg ttagaatagc cgattcggag agtctctccg
    31381 agatattctc tacgcttaca gctgtctgcc cctgctagaa aaccaaggcg aatcaagtcg
    31441 ctacgactaa tgtcaaactg actctcatgt tcaaattgtt ctgtcacctg agctagagcc
    31501 gttttcaggt cttcatagct ggcatgctca attcccagag aacgcccctt ggtcttggac
    31561 ttgggaacag cttcatctcg cttgagaaag gcatgctgaa ctgttggaat gaccatcatg
    31621 atcatgcgcc gaatccgttc cccattaaaa tctgggtctg taaagacaat gactccatga
    31681 cgttggtgca ggcgctgaat ccgctctata tcctgagcat tgatggcaga acctcgagtc
    31741 tcataggtct ccacatcgaa ataacgtttg agattgaccg tatcatcacg cccttcaacc
    31801 acgataactt gagaaatttt ctctttcatt acttgctgtc cagtccaaaa attcgttctg
    31861 catttgcagt cgttgctacc gccagctctt ctgtcgtcat accacgcaag tcagcgataa
    31921 agtcgaccac atagcgagta taggctgttt tattttcacg accacgcttg ggtacaggtg
    31981 ctaagtaagg cgcatctgtt tccaccaaca tcttgtccaa aggtaactct ttagctgctt
    32041 cttggaggtc agttgccttc ttaaaagtca ccactcctga gaaggaaatg gtcataccaa
    32101 gatccacaaa cttctctgcc cactcaagcg tccctgaaaa tgaatgcatg ataccaccac
    32161 gaggaccaac gccctcactc ttgataatct cataggtatc ttccagcgca tcacgggtat
    32221 ggacaacaaa aggcaaatcc aagtccttag atagctgaat ctgacggcga aaaacctgct
    32281 cctgcacctc tttgggcgct gtcatccaat ggtaatctaa gccaatttca cctaaagcca
    32341 caaccttgga atgttttaac ttatccaaca agtaagcctc aacttcctct gtataagtac
    32401 cagcttctgt aggatgccaa ccaatagtcg catagagctg ctcatactca tctaccaact
    32461 ccaaggcatg ctcaatcgtc ggtttatcaa aaccaacaat attcatctgt gtcacaccca
    32521 tctcagcagc caaggcaatt tcttctgcct cacgacctgc aaattcttct acattcaagt
    32581 gtgtatgtgt atcaaaaatc atctcttcta acctcgtttt ctatcttcta ttataccaaa
    32641 aaagaggagg ggcacctaat ttttcggttt cccctcctct cttcaataga gagctattct
    32701 gctatctttt ctatccgata ttgcccatct cctattccac agttagagac agaagagatt
    32761 ggctacattg atagaatatt cctattcagc taaagtatgt gattatcttg ggttctcact
    32821 tttctcaccc atcactagga ttgatgactt ctcaaaatcc cccttataat ctaactttaa
    32881 gtagagaata gaattttcta gctgttctat tttctacgtc tagtctttct tcctagcaaa
    32941 gcaaaaccac ttgttgccac cactagacca agactagcaa gtgctgcact tgatgctgaa
    33001 cctgtgttag gtaattgttt gccagcaggt gactggtagg taacatcttg cattgttcct
    33061 gcagttgctt gcggtgcact tgttgtctta ggagcagcat taggagccac tggtctctta
    33121 ggaactgtaa ccatggcaat ggcttgcact ccaatcgttt ctgcttcctt cacatcttcc
    33181 atagttttcg cattttcaat ctctttgaga gctgcttgtt tttctgcttc cactcttgct
    33241 aaaagttctg ctttttcttt atcagaaagc ggtgcgcctt tgatttcatc ctgcttgtcc
    33301 ttggctgctt tttcaatggc atttttagct gattcttttt cagtagctaa ttgcttagaa
    33361 gcttgtgatt gagctgagtt ttgtgaacct gtgtgagctg atccacccgc ctggctacca
    33421 ccgccttcat tcgcattagc atctgagctt ccagtattgc ctcccttatc tccacctggt
    33481 gtattttcta gcttatactc tggtgtagct tcttgagtta cagattcacc tttcgcagat
    33541 tgttgaactg tatcttttcc tagaatcgtc actactgaac catctgggaa tgtgattgtt
    33601 gctgtaccat ctccagctac attgattgtc gctccgtcta atgctgaacc atttgcttgt
    33661 aaaatagcaa ccttaacttt ttcttcatct gttaaatgag ttatatcttt tacatctact
    33721 ctttgagcag gtgctttaac agttgatttt tcagcagtca gtttagtgct atcttgtgct
    33781 tttggatctt gtgctaccaa ttgatttcct ggaattgtaa tagctgaacc atctttaaag
    33841 acgattgttg ctgtaccatc tccacctact tcaactttat agtaaaataa aataataaca
    33901 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaaa cagaggtgta
    33961 ctattctagt ttcaatctac tgtaacatcg cttccgaatc cgtttgcatc tactgaactt
    34021 ttttacaaaa ataaaaatca gtagactcga gttcctactg atttttgata gaattcgttt
    34081 ggattatgca gccaaaactt tgcgtccttt acgacgacga gctgccaata cgcgacgacc
    34141 atttttagtt gacatacggt tacggaatcc gtgtttgcgc gcacgacgaa gtttacttgg
    34201 ttgataagta cgtttcacga tgaatacctc ctcatagatt ttgtattcgt ttagccggct
    34261 ataaagtgat cagttactaa acatacttta ctattctatc tgattctttc acttttgtca
    34321 atagctctag gcaaatgttt ccagcttttt cgaatgcaag ccctatctac gatactgttt
    34381 caagaaacga gtcatctttt cttggatttg ggctaactca tcaacacgag gaaggtaaac
    34441 gatacggaag tggtctggtt cctgccagtt aaagcctcga ccatgaacca agagaacctt
    34501 ttcctgcttc aagaaatcaa ggacaaactg ctcatcatca tcgatacggt acatattgcg
    34561 gtcgattttt gggaagatat agagccccgc cttgggttta acggcagaca aacctggaat
    34621 atcttgaatg gcattataga tgaaatttct ttgctcgtag attcgtccac caggaagaag
    34681 caattcatcg actgattggt gaccccccaa ggaagtttgt acgacttgtt gggccaaaac
    34741 gttagagcaa aggcgcatat tggacagcat attgagccct tcgatatagc ccttaacatg
    34801 agtcttaggg ccagacaaga ccatccaacc cacacggaaa cctgctatgc ggtgggattt
    34861 tgacagacca ttcatgctga cacagaagac atctggtgcc aagctcgcca caggcgtatg
    34921 cacatgtccg tccattacca tgcggtcata gatttcatcc gcaaagataa tcaaatcgtt
    34981 ttgacgggca atctcaataa tctccagcaa gagttcctta ggataaaggg ctccagttgg
    35041 gttattggga ttgataagga caattgcctt ggtattggaa gtgatttttg acttgatatc
    35101 gtcaatatct gggtaccatt ccacagcttc atcacagata tagtgaacgg catttcctcc
    35161 agctaggctg acagccgccg tccatagagg atagtctggc attggcacta agacctcatc
    35221 cccattgtcc aaaagcccct gcatggacat gacaatcaac tcactgacac catttccaag
    35281 gtaaatatca tcaatatcta cgtttgggaa tttcttcagc tggcaatact gcatgatggc
    35341 cttacgggct gagaaaatcc ctttggagtc agaatagcct tcactatcac gcgcattcat
    35401 aatcaagtca tgaatgacct cgtctggcgc tgtaaagcca aattctgctg gatttcctgt
    35461 attcagacgt aaaatctttt ctccattcgc tcgcatccgc atggcttctt ccaatacagg
    35521 accacggata tcataagcaa catgctctaa cttactagac ttgttatatt ctttcatctt
    35581 gtttcctcaa ttcatcagga tagtaacatt ataccactag aaagagaatg cgacaagttt
    35641 actgaaatgt gttactagtt tcgtatagaa gaaaaacctt gctaaaggcg aggttaagaa
    35701 cgtagagata gcaggtattt tgcaaagaca catcacatac caaaagataa acctagaaaa
    35761 aagcaagcct agaaaggagt cataaagaag attaaacatg aggaaaagcc cccatatcat
    35821 caagtaatcc caacgcccac ttcgttttgc aactagaaat aagattagat ggtagagtac
    35881 atgaaaaact aaaaatccaa taaaacttgg ataaagaagc ggtaccagac tctctaattg
    35941 acagatcatg atcacttcta ccaagactga aactaggata attccataat aaaggaaatt
    36001 tgatttttga aaagagtttt tcatcattct cccttccttc acttctttcc tgctatcctt
    36061 tcttttattt tatcatatat ttatatagag tttaagccct aatattcttt tgaaaaatac
    36121 tttttttgct ttacttctat agtgaaaagg tttacaatta tagtaagaaa atttcaggag
    36181 gtttcattat ggcacaacgt taccaaaata tcatggtcgc aatcgatggt tctaaggaag
    36241 cggacttggc ttttgtcaag ggagttcatt ctgctctacg aaacgacgct aaactcacca
    36301 tcgcacatgt cattgacaca cgcgctctcc aaagcgtatc cacctttgat gctgaagttt
    36361 acgaagaact ccaagtcgac gctgaaagtc tgatgaaaga gtacgaaaaa cgtgctaaag
    36421 atgctggggt agcagatgtt catatcgtca ttgaaatggg aaatccaaag accctgctag
    36481 cacgtactat tccagatgcc gaggaagtgg acctcatcct cgttggcgca actggtctca
    36541 acgcctttga acgcctcttg gtcggctctt catctgaata catactccgc catgctaagg
    36601 tcgatttgct ggttgtgaga gaacaagaaa aaaccttata atcacaaaga aaaggagccc
    36661 ctagctcctt tttgtttacg atttatttct ctctttatgg cgttcgtaag ccttgagctg
    36721 gcgctgcagt tcctttttaa tagcaggttc tggagcatat ttttcttccc aattatctgg
    36781 ttttaagatt ttatgggtca ctggatcaaa atgagccttg ccatctggaa aaattttccc
    36841 catattggcc tgatggacaa tatcaaaaat acgttctggg tccaccccca tcaagacaaa
    36901 actgccgtag gtgaagtaaa gcgtgtcaat caaggcatcc acttgcccta tcaaatcttg
    36961 ctgagcaggt gtcttcttgg ctactttacc tgctgcctta tcaagggcct gatgaagttg
    37021 cgatacagct tgaccaaaat cttcttcaga aggactggct gatcgaacaa actccaccaa
    37081 ttcttctatt ttaaaaccag ccctatgggt tgcaccctct aaatcccaag ctcgaggttc
    37141 ttcttgggtt cgttcatcca tcatgtggtg gaaggtcttg accttattga aatgatagtc
    37201 acggctgaca aaaacttttt ctgaagacaa aacaccaaaa tgttccaagg ccttgtggat
    37261 cccatcttgt tgattgctag tggtaatgtg cttggcagct tccttgacac tgctactacc
    37321 atttcccata gcgaccgaca taccgacgcc agccagcatt tcaaggtcat tatctgagtc
    37381 accaaaagcc atgacttggt tgaggtcaaa gccatattct ttcccaactc ggcgaatgcc
    37441 ttctaattta gaatttccct gattgatgac atccgatgca aaaggattgc tacgtgtcaa
    37501 tttcaagtct tcaaaatcag ctgccgcctt ctcagattct tctggtgtca tcagcatcaa
    37561 aacttggtaa ataggttgat tcatcaggtg aagcaggtcc tcttcctttt ggggaacaac
    37621 cttgctgacc atgcgattaa aagactgact caccgtccga gttaaaacag agggaacgaa
    37681 gcgactaatt cgttgggaaa aagaacccag accaaaggac atgattttag aacccaacat
    37741 ggcatccttg gtccctaagg caatctccgt gccctctttt ttagcatagc taattagatg
    37801 gcgcaaatgt aacttggaaa tagggctcgt gaacaagact ctgtctttac taaagatata
    37861 ctggccatta taggttaccg caaaatccag atccaaatcg tccatcaatt ccttaacaaa
    37921 aaaaggtcct cgccctgtcg ctacgccaac tagtacccct tgttctttga caatcttaat
    37981 cgcgtcctta gtggatttca aaacactctt gcgattgttg accaaggttc catcgatatc
    38041 aaaaaaaaca gctttgactt ccatcctatc ccaatctccc cttttgtgat acaatgatta
    38101 taccacattt cagaaagagt gagtaaatca tgcctaagaa aatccttgtt ttacatacgg
    38161 gtggaactat ttccatgcag gccgatgctt ctggcgctgt tgtgacgagt tcagataatc
    38221 ccatgaacca tgtgtccaac ccacttgaag gaatccaagt ccacgccttg gactttttta
    38281 accttccaag tccccatatc aaacccaaac atatgctggt cctctaccag aaaattaaag
    38341 aggaagcaga taactacgat ggagtggtga tcacacacgg aaccgatact ttagaggaaa
    38401 cagcctattt ccttgatacc atggaagttc cccatatgcc tatcgttcta acaggagcca
    38461 tgcgtagctc caatgagctc ggtagtgatg gtgtttataa ttacctaagt gctttacgag
    38521 tggccagcga tgacagggct gctgacaaag gagttttggt cgttatgaac gatgaaatcc
    38581 acgctgccaa gtatgtcacc aaaacacata cgactaatgt cagcaccttc cagactccaa
    38641 cacatggccc cctcggtctc atcatgaaac aggaaatcct ctacttcaaa acagctgaac
    38701 ctcgtgttcg ctttgacctt gatcacatac aaggtttagt ccctatcatc tcggcttatg
    38761 ctggtatgac agatgagctg attgatatgc tggatttaga acacttggac ggtttgatta
    38821 tccaagcctt cggagctggt aatattccca aagaaacggc tcaaaaatta gaaagccttc
    38881 tgcaaaaagg aattccagtc gctctggtat cacgatgctt taacggtatt gccgagcctg
    38941 tttatgccta ccagggtggg ggcgtacagt tgcaaaaagc aggcgttttc tttgttaaag
    39001 aactcaacgc ccaaaaagct cgcttgaaac tcctcatcgc cctcaatgcc ggactaacag
    39061 gacaggcttt gaaaaactat atggaaggct aatactcttc gaaaatctct gcaaaccacg
    39121 tcacgtcgcc ttaccgtatg tatggttact gacttcgtca gtttcatcta caacctcaaa
    39181 aacatgtttt gagctgactt cgtcagttct atctacaacc tcaaaacagt gtgttgcgca
    39241 gcctttaacc agtcgcctag tccgctctat ggtattcatt aagtcaacat ctcttgttta
    39301 agagcacaaa atcaggaaat cttctcgatt ccctgatttt ttctatttac gttttcgtgt
    39361 tgagctacgt tctgtcaaac catgaggtaa gagaacttca cgttcttcca actcttcctt
    39421 atgcataatc ttggtcaaca tacgcatact aatggcacca agatcataaa gaggttgggc
    39481 aatcgttgtc aagtttggac gggtaaagcg tgagatttgt gaatcatcac tagtaataat
    39541 ttcaaaatct tctggcacag aaacaccctt atcagccaaa ccgttcaaga ctcctgctgc
    39601 caactcatca cctgtcacaa ctgctgcagt tgcatttgat gaaatcaaac gctctgctaa
    39661 ggcgtaacca tcatcatagc tatatttaga ttcaaatacc aaaccctcac tataagtgat
    39721 tcctgctttt ttcaaggttt ccttgtagcc aactaaacga accttaccat tgatgtcatc
    39781 cactagcgga ccgctaacga aagcaatacg ctcattttct ttagcaaggt aactcactgc
    39841 atcaattgtt gcttgcttat agtcaatatt gacacttggc aactggtgct caacatcgac
    39901 agttcctgcg agaacaatcg gagtacgtga acgcgaaaat tctgagcgaa ttttatctgt
    39961 caagtgataa cccatataga taatgccatc tacctgcttt gaaaagaggg tattgacaac
    40021 agaaacttct ttctcgttat cttcatcgct attagctagg acaatattgt acttgtacat
    40081 ttctgcaata tcatcaatcc ccttagccaa actcgaaaaa taaccattgg taatatttgg
    40141 aatcacgaca ccgacagtgg ttgtcttttt acttgcaaga ccacgcgcaa ctgcatttgg
    40201 acgataatcc aaacgatcaa ttacctctag cactttttta cgggtattct cttttacatt
    40261 tttattgcca ttgaccacac ggctgaccgt cgccatggaa acacctgctt cacgagcgac
    40321 atcataaatg gttactgtat catctgcatt cattcctttt cctgtccttt ctatctcaca
    40381 cattctttta caagtagagg tactgattga agctctatat ctacttacaa aagtgaagat
    40441 gtgaaaattt cgttttcata tttctactta ttccattcta tcactaattg taaacacttt
    40501 caagtgtttt ttgaagattg attgaaaaaa tttcatagaa aacctaggtt tagctccttg
    40561 ctaccacctt agactaaaca caaaggagga aactaagccc tcctaaagtt atagtaaaat
    40621 gaaataagaa caggataaat cgatcaggac agtcaaatcg atttctaaca atgttttaga
    40681 agtagaggtg tactattcta gtttcaatct actataggta ttgttccatt cactaccgtc
    40741 aattttagca catagccttc ataaaaatat tatatcatca taaccaacca gattcttttg
    40801 cgatattagc tgcctctgtt cgattaccag catccagttt tgaaagaata ttggtgacat
    40861 agtttcgaat agttccgttt gagaggtaaa gctgatctgc gatttcttgg ttggataagc
    40921 cccgggcgat tccctttaag acagcaatct cttgttctgt caacggattg ggacgggtca
    40981 tcaccacttc catcaattca ggcgaatact ccttgcgtcc ttcgaggacg gtgtgcaagg
    41041 tttgcatgag gtctgcaatg cttcgttcct ttaatacata agcatccact ccagccttga
    41101 ccgcacgttc aaaataccca gcacgcttga aggtcgtcac cacaaccacc tttgtttcaa
    41161 gcctttctga tcgtatccac tccaagactt caagacctgt cttaacaggc atttctacgt
    41221 caaggatggc gatatctaca gactcctttt ctaatagttg gattgcttct tgcccattct
    41281 tggcttgaag gacagactcc acatctggtt gaagcgtgag caattgacac atggcatctc
    41341 gcaacatact ttgatcttct gcgactaata ctttcatctt agtttctctc cttataaggt
    41401 agtcgaactt gcacttccgt tggatgtttc tgactgatta cacttacttc tcctgaaaat
    41461 ggaaaaacac gatctcggac tgtatggagc tcatctcctt ttagaaaagc aaagccgcag
    41521 ccatcatcac ttactgttaa aatgagttcc ttctctgtcc gttctaattt taagtaagct
    41581 ttagacgctt tggcatgttt gatgatattg gtcaccaact caagcaaaat catagaagtc
    41641 attgcctcca attcttgagt aaggctagca gtatctagtt ggttatccgt ttccacctca
    41701 attccagcaa tttctaacat ctttttcaca gtctctagtt cggatgtcaa agttctagac
    41761 ttaagatttt ccacaatggt tcgaacttca cgcatcgatt ctttgctaat ttgctgtatt
    41821 tctcttaatt ccttttccac ttgtggataa gcctgcattt gaaatagctg caaggctaga
    41881 tctgtcttga cactaagcat agcaaaggta tgtcccagac tatcatgcaa atcctgaccg
    41941 atacgattgc gctcattttc tgctagcaat agatttatct gggtattttg ctcgacctga
    42001 gcttctttca aatcctcgac aaggcgaatt cgaaccgagc ccaaagtcat caaatcgaca
    42061 aaagtaagaa ttacaagcat atagactaga aactcaactt cgattctctg aaaaatcaaa
    42121 agtcgcccca caacaaggac ttgagcaagg agaaatgtcc atacatgtaa agactttaaa
    42181 ctacgtacgc caaaatgata aattaagaga ttggaaagga aaaagaaaaa ccagatataa
    42241 ttcccagcaa caaaggcggt attcccagct acatagacca gcatgatccc ccagaaaagc
    42301 caagataggc gctggttctt agttattaaa acacccaaat acgtcactac aaatagaata
    42361 tcaaccaata aatgccaagc aggaagctcc ccaatcacta caggtaggat gggaaaaagc
    42421 ataaaaatca aactgaccca aaacatatag tgtatgcttt tcagtctttc aagcattaag
    42481 cattctcctt atgaccttga aggtaaatag tcaaaccaaa taaaactgct gaaaaaacaa
    42541 gtaaataaac tgtggctgat agattgatgc caccctcgtt taagaaggtc ttgagcaact
    42601 ccatcaattg ataggtcggg agacacttac caactgcttg catccagtct ggaaataaag
    42661 aaatgggcat ccagagtccg cctaaaacag ccaatcctag ataaagaaga ttgcccacaa
    42721 cagacatcag ctgactagtt ggtaagagag tcaaggtcaa gccaagtgct acaaaagcaa
    42781 tactccccac aatcagcaaa atcgcagctc caacccattt tactagaggc atgtctacac
    42841 ctcttacaaa atgtccaact gtaaaaacaa ccagaattga gaccaaataa tcaaccagca
    42901 tacttgttat ctttgataga taatattcta ccatatttac agggctatga cgcaatgttt
    42961 tctgccagtt gttcatctta tcggtatgta aaacagctgg aaatgagagg atagttgtcg
    43021 acatcatgga aaaggcagtc atggagatga ggtaatcacg cataaaattc gcgggttcac
    43081 ctggtgtgtc ctgatagata ccagaaaaaa ataaatagaa agccgtcggc atccctaccg
    43141 acaatagata atagattaat tgtcgtttgg tcaataaaaa ttctatctta ctaagtgcta
    43201 tccatcgttt catcttagtt atctcccttc tgcgtctctt caaagattgt attcaacaaa
    43261 ctacgattat taacttcgat ttcttgcatc ctacatcctg cttgggctaa tagttcccag
    43321 aaagcatctg cttctcgagt gactacttgt aaagaatctt gctttagggt ccagttttca
    43381 accaaatttg actgctctac gacttccttg taagctatag gaaggataaa gtgcttttct
    43441 atttcctcgc tacgcatagc tagaggcgtc gtatctcgaa tcaactctcc cttatttaag
    43501 agcaaaatgc ggtctgcagt gtgttccact tcctcaatat aatgggacga atagagaatg
    43561 gtgactcctt gcgcttttaa ctcctggaca atttcccaaa aacgttgacg agttgaggta
    43621 tccatggacg cagtaggctc atctaaaaaa acaatctttg gtcgcccaat taaggtcaag
    43681 atgaaagaaa agagacgctt ttgaccgcct gataattttt ctgccaactg ttctttttgt
    43741 tgcttgacaa actgcaatag ttgatcaatt tcctgattgc tcaaaggatt tggatagata
    43801 cgttgaaaga aagcaatcaa ctctttaact tttaatttct gaacgattac attttcttga
    43861 gggaggtaag ccctagtata gtctaactta gaactcgtca ctggtaaacc ttgaatagat
    43921 acttgaccgc ttgtgaccag tttatctcca agcaaacaat ctaagagtgt tgtctttccg
    43981 gcaccatttg gaccaattaa ggcaacacat tcaccatcgt tgatttcaaa ggagatgttc
    44041 cgcaaaattt ccttgtcttt tattttctta ctcaatttct caactttaac caccgtcatg
    44101 agactctcct ttcaaccact ccattcccat agggaaaacg acgaaaatca taaatccaaa
    44161 ccccaaagca ccatgaatga attggcgaag caaggcttga tcaaaccaat cagtaaacat
    44221 ttccactaac cataccaaga gtgacaggcc gataaagaaa tagatgatcc ctctcttcat
    44281 tcctcaaact cctttttcac atctctgact aatttcaaac cttctctaac aagccaagac
    44341 atgattccaa agccagcaaa gaccttccaa ggaaaatggt agaaatcctc atccaatccc
    44401 gaaaacatga gataggtcat aactcctgct actactaaac tcattgcaat aatcatttta
    44461 tttctcatct cttcttcccc catttcatac tacaattata gtcttttaaa atcagaggag
    44521 gcagaagctt ctgtcactag aaaatatgac aaatgtcata aaaaattctg ttcaaaacaa
    44581 gcaagataca ctatacaata aaacacaatc agaaaaatct aaggcaactt cctcaaaaga
    44641 gatatcaaac tcaattcaca ccataatgta aactaatact tatttaaaat caaaaagagt
    44701 agaaattttt atcagacaaa cacat
//
LOCUS       DAVKQB010000011        44231 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKQB010000011 DAVKQB010000000
VERSION     DAVKQB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44231
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.011"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            201..770
                     /locus_tag="VW528_000593"
     CDS             201..770
                     /locus_tag="VW528_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9911455.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            838..1488
                     /locus_tag="VW528_000594"
     CDS             838..1488
                     /locus_tag="VW528_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU9911456.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            1472..3712
                     /locus_tag="VW528_000595"
     CDS             1472..3712
                     /locus_tag="VW528_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9911457.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            3892..4080
                     /locus_tag="VW528_000596"
     CDS             3892..4080
                     /locus_tag="VW528_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911458.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGSY"
     gene            4112..4699
                     /locus_tag="VW528_000597"
     CDS             4112..4699
                     /locus_tag="VW528_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911459.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMTDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            4703..5878
                     /locus_tag="VW528_000598"
     CDS             4703..5878
                     /locus_tag="VW528_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911460.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWKDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    6052..6183
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            6199..6729
                     /gene="infC"
                     /locus_tag="VW528_000599"
     CDS             6199..6729
                     /gene="infC"
                     /locus_tag="VW528_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9911461.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            6762..6962
                     /gene="rpmI"
                     /locus_tag="VW528_000600"
     CDS             6762..6962
                     /gene="rpmI"
                     /locus_tag="VW528_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9911462.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            7014..7373
                     /gene="rplT"
                     /locus_tag="VW528_000601"
     CDS             7014..7373
                     /gene="rplT"
                     /locus_tag="VW528_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9911463.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            7431..7811
                     /locus_tag="VW528_000602"
     CDS             7431..7811
                     /locus_tag="VW528_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9911464.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            8061..8861
                     /locus_tag="VW528_000603"
     CDS             8061..8861
                     /locus_tag="VW528_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9911465.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVNQRVCEDGPVFRTGTVVL"
     gene            8824..9810
                     /locus_tag="VW528_000604"
     CDS             8824..9810
                     /locus_tag="VW528_000604"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9911466.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            9852..11753
                     /locus_tag="VW528_000605"
     CDS             9852..11753
                     /locus_tag="VW528_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU9911467.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     AKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWL
                     DKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIP
                     VASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINN
                     SLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYL
                     SAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMK
                     INEKLGGKD"
     gene            complement(11814..13469)
                     /gene="pavA"
                     /locus_tag="VW528_000606"
     CDS             complement(11814..13469)
                     /gene="pavA"
                     /locus_tag="VW528_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9911468.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            13574..14071
                     /gene="ybeY"
                     /locus_tag="VW528_000607"
     CDS             13574..14071
                     /gene="ybeY"
                     /locus_tag="VW528_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9911469.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLGYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            14052..14447
                     /locus_tag="VW528_000608"
     CDS             14052..14447
                     /locus_tag="VW528_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9911470.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            14464..15363
                     /gene="era"
                     /locus_tag="VW528_000609"
     CDS             14464..15363
                     /gene="era"
                     /locus_tag="VW528_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9911471.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            15412..16236
                     /gene="mutM"
                     /locus_tag="VW528_000610"
     CDS             15412..16236
                     /gene="mutM"
                     /locus_tag="VW528_000610"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9911472.1"
                     /translation="MPELPEVETVCRCLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            16236..16841
                     /locus_tag="VW528_000611"
     CDS             16236..16841
                     /locus_tag="VW528_000611"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9911473.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            16828..18027
                     /locus_tag="VW528_000612"
     CDS             16828..18027
                     /locus_tag="VW528_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU9911474.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            18027..18176
                     /gene="rpmG"
                     /locus_tag="VW528_000613"
     CDS             18027..18176
                     /gene="rpmG"
                     /locus_tag="VW528_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9911475.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            18216..18449
                     /gene="secG"
                     /locus_tag="VW528_000614"
     CDS             18216..18449
                     /gene="secG"
                     /locus_tag="VW528_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9911476.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KVRGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            18551..20905
                     /gene="rnr"
                     /locus_tag="VW528_000615"
     CDS             18551..20905
                     /gene="rnr"
                     /locus_tag="VW528_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9911477.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAANYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            20868..21335
                     /gene="smpB"
                     /locus_tag="VW528_000616"
     CDS             20868..21335
                     /gene="smpB"
                     /locus_tag="VW528_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9911478.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            21351..22211
                     /gene="tehB"
                     /locus_tag="VW528_000617"
     CDS             21351..22211
                     /gene="tehB"
                     /locus_tag="VW528_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9911479.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            22284..23237
                     /gene="coiA"
                     /locus_tag="VW528_000618"
     CDS             22284..23237
                     /gene="coiA"
                     /locus_tag="VW528_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9911480.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            23256..25058
                     /gene="pepF"
                     /locus_tag="VW528_000619"
     CDS             23256..25058
                     /gene="pepF"
                     /locus_tag="VW528_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9911481.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEVLKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            25060..25773
                     /locus_tag="VW528_000620"
     CDS             25060..25773
                     /locus_tag="VW528_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9911482.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELENFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            25840..26781
                     /gene="prsA"
                     /locus_tag="VW528_000621"
     CDS             25840..26781
                     /gene="prsA"
                     /locus_tag="VW528_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9911483.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFKKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(26898..27239)
                     /locus_tag="VW528_000622"
                     /pseudo
     CDS             complement(26898..27239)
                     /locus_tag="VW528_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            27652..27747
                     /locus_tag="VW528_000623"
                     /pseudo
     CDS             27652..27747
                     /locus_tag="VW528_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(28000..28620)
                     /locus_tag="VW528_000624"
     CDS             complement(28000..28620)
                     /locus_tag="VW528_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9911484.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(28620..29102)
                     /locus_tag="VW528_000625"
     CDS             complement(28620..29102)
                     /locus_tag="VW528_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9911485.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(29102..29944)
                     /locus_tag="VW528_000626"
     CDS             complement(29102..29944)
                     /locus_tag="VW528_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9911486.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(30039..30839)
                     /locus_tag="VW528_000627"
     CDS             complement(30039..30839)
                     /locus_tag="VW528_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9911487.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            30983..32362
                     /gene="glmU"
                     /locus_tag="VW528_000628"
     CDS             30983..32362
                     /gene="glmU"
                     /locus_tag="VW528_000628"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9911488.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            32372..32917
                     /locus_tag="VW528_000629"
     CDS             32372..32917
                     /locus_tag="VW528_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9911489.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            32929..33243
                     /locus_tag="VW528_000630"
     CDS             32929..33243
                     /locus_tag="VW528_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9911490.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAAIFLLILLVLAMK
                     LL"
     gene            33260..33952
                     /locus_tag="VW528_000631"
     CDS             33260..33952
                     /locus_tag="VW528_000631"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9911491.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            34029..34520
                     /gene="rocS"
                     /locus_tag="VW528_000632"
     CDS             34029..34520
                     /gene="rocS"
                     /locus_tag="VW528_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9911492.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            34618..35208
                     /locus_tag="VW528_000633"
     CDS             34618..35208
                     /locus_tag="VW528_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9911493.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAD
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            35217..36980
                     /locus_tag="VW528_000634"
     CDS             35217..36980
                     /locus_tag="VW528_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9911494.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            37194..37839
                     /locus_tag="VW528_000635"
                     /pseudo
     CDS             37194..37839
                     /locus_tag="VW528_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            38006..38206
                     /locus_tag="VW528_000636"
     CDS             38006..38206
                     /locus_tag="VW528_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911495.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            38231..38647
                     /locus_tag="VW528_000637"
     CDS             38231..38647
                     /locus_tag="VW528_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911496.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLDVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            38878..39585
                     /gene="ccdA2"
                     /locus_tag="VW528_000638"
     CDS             38878..39585
                     /gene="ccdA2"
                     /locus_tag="VW528_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9911497.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            39596..40153
                     /gene="sdbB"
                     /locus_tag="VW528_000639"
     CDS             39596..40153
                     /gene="sdbB"
                     /locus_tag="VW528_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9911498.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            40274..41665
                     /locus_tag="VW528_000640"
     CDS             40274..41665
                     /locus_tag="VW528_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9911499.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            41808..42725
                     /gene="adcAII"
                     /locus_tag="VW528_000641"
     CDS             41808..42725
                     /gene="adcAII"
                     /locus_tag="VW528_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9911500.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            42733..>44231
                     /locus_tag="VW528_000642"
     CDS             42733..>44231
                     /locus_tag="VW528_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9911501.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITYRTVNGVAVPHGDHYHFIPYSQLSPLEEKLA
                     RMIAVKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQVVRKVGEGYVVEIAGV
                     SHYVFAKDLAKDKIDAIENLLSKKTQETHALVAKKENVAPRDQEFYDKAYNLLTQAHK
                     VLSENKGRTSDFQALDKLAERLNNESSNKVKLVDDLLAFLAPITHPERLG"
BASE COUNT        13528 a         7987 c         9674 g        13042 t
ORIGIN      
        1 ttcttttggg attccgatta acattgagat aacctacctt tcaattgacg gtcttgtttt
       61 ggttgtcaca ttccagttca taaatcaaaa atgtgacggt ttcattgtat atgaaaccgc
      121 ttcaaaaatc aagaaaaact tgtcatccaa atttttttat gctagactag tgaaaatcaa
      181 gctctaatgg agggaaaagt atggaatcaa tatttgtgaa atttgcccag tatccgtcta
      241 tagaaacgga gcgtttattg cttagacctg taactttgga tgatgcggaa gcaatgtttg
      301 actatgcctc ggacaagggt aatacacgtt acacttttcc aaccaatcaa agcttggaag
      361 aaaccaagaa taacattgct cagttctact tggctaatcc cttgggacgt tgggggattg
      421 aattaaaaga taacggcaag tttatcggaa ctattgactt acacaagatt gatcctgttc
      481 ttaagaaggc agctattggc tacattatca ataaaaagta ttggaatcaa ggattaacga
      541 cagaagccaa tcgtgctgtg attgagctag cttttgagaa gatagggatg aataagttga
      601 ctgcccttca cgataaggct aatcccgcgt caggaaaggt catggagaaa tcaggcatgc
      661 gtttttccca tgcagaacca tatgcttgta tggaccagca tgaaaaaggc cgaatcgtga
      721 caagagttca ttatgtcttg accaaggaag actattttgc aaataaataa gcagttgaaa
      781 agaaattttt cgactgtttt ttcttcctct tacgaataat ctaagagagg agaaaatatg
      841 gaagcaatta tcgagaaaat caaagagtat aaaatcatcg tcatctgtac tggtctgggc
      901 ttgcttgtag gaggattttt cctgctaaag ccagctccac aaacatctgt caaagagacg
      961 aatttgcagg ctgaagttgc agctgtttcc aaggactcat cgaccggaaa ggaagtgaac
     1021 aaggaagaga aggaagaacc agttgaacaa gatctaatca cagtagatgt caaaggtgct
     1081 gtcaaatcgc cagggattta tgacttgcct gtaggtagtc gagtcaatga tgctgttcag
     1141 aaggctggtg gcttgacaga gcaagcagac agcaagtcgc tcaatctagc tcagaaagtt
     1201 agtgatgagg ctctggttta cgttcctact aagggagaag aagcagttag tcaacagact
     1261 ggttcgggga cagcttcttc aacaagcaag gaaaagaagg tcaatctcaa caaggccagt
     1321 ctggaagaac tcaagcaggt caagggactg ggaggaaaac gagctcagga cattattgac
     1381 catcgtgagg caaatggcaa gttcaagtca gtagacgagc tcaagaaggt ctctggcatt
     1441 ggtgccaaga ccatagaaaa acttaaagac tatgttacag tggattaaga atttctctat
     1501 tcccctaatt tacctgagtt ttctgttact atggctttac tacgccattt tctcagtatc
     1561 ctattttgct ttgttgggtt ttgtttttct gctagtctgc ctctttatcc aatttccttg
     1621 gaaatcagca ggtaaagttc tagtgatttg tggagtcttt ggcttctggt ttctgtttca
     1681 aacttggcaa cagagtcaag tgagtcaaaa tttggtggat tctgttgaaa gggtacggat
     1741 tttaccagac actattaagg ttaacggtga cagtctgtcc tttcgtggta agtctgatgg
     1801 tcgtgctttc caagtctatt ataaactcca gtccgaggag gagaaagaag cctttcaagc
     1861 tttaactgac cttcatgaga taggactaga agggaagctt tcggagccag aagggcagag
     1921 aaattttggt ggctttgact accaaggcta tctgaagact cagggaattt accagacact
     1981 caatattaaa agaatccagt cactccaaaa ggttggcagt tgggatatag gtgaaaacct
     2041 gtccagttta cgtcgaaagg ctgtggtttg gattaagatg cacttcccag accctatgcg
     2101 caattacatg acaggactct tgctaggaca tctggacacc gattttgagg agatgaatga
     2161 gctttattcc agtttaggaa ttattcacct ttttgccttg tcaggtatgc aggtagggtt
     2221 tttcatggac ggatttaaga aactactttt acgattgggc ttgacacaag aaaagttgaa
     2281 gtggctgact tatccctttt cccttatcta tgcgggacta actggatttt cagcatcggt
     2341 tattcgcagt ctcttgcaaa agctactggc tcaacatggg gttaagggct tggataattt
     2401 tgccttgacg gtgcttgtcc tctttattgt catgccaaac tttttcttga cagcaggagg
     2461 agtcttgtcc tgtgcttatg cttttatcct gaccatgacc agcaaagaag gggaggggct
     2521 caaggctgtt actagtgaaa gtctagtcat ctccttgggc atattgccca ttctatcctt
     2581 ctattttgcg gaatttcaac cttggtctat ccttttgacc tttgtctttt cctttctttt
     2641 tgacttggtc ttcttaccgc tcttgtctat cttatttgtc ctttcctttc tctatccagt
     2701 cattcagctg aactttatct ttgaatggtt agagggcatt attcgcttgg tctcgcaggt
     2761 ggcaagtaga ccgcttgtct ttggacaacc caatgaatgg cttttaatcc tattgttaat
     2821 ttctttggct ttggtctatg atttgaggaa aaacattaaa aagctaacgg tattgtgctt
     2881 attgattaca gggctctttc tcctgaccaa gcatccactg gaaaatgaaa tcaccatgct
     2941 ggatgtgggg caaggagaaa gtattttcct acgggatgta actgggaaaa ccattctcat
     3001 agatgtaggt ggtaaggcag aatcttataa gaaaatcaaa aaatggcaag aaaagatgac
     3061 gaccagcaat gcccagcgaa gcttgatacc ctatctcaaa agtcgaggag tagctaagat
     3121 tgaccagcta attttgacta acacggacaa ggagcatgtt ggagatttgt cagagatgac
     3181 caaggctttc catgtagggg agattctagt atcaaaagac agtctgaaac agaaggaatt
     3241 tgtggcagaa ctacaggcga ctcaaacaaa ggtgcgtagt atgatagtag gggagaactt
     3301 gcccattttt ggaagtcagt tagaagttct atctccaagg aaaatgggag atggaggaca
     3361 cgatgatacc ctagttctgt atgggaaatt cttggataag caatttctct tcacgggaaa
     3421 tttggaggag aaaggagaga aggacttgct gaagcactat ccagacttga aagtaaatgt
     3481 tttgaaagct agccaacatg gcaataaaaa atcatcaagt ccagcctttc tagaaaaact
     3541 caaaccagag cttactctta tctcagttgg aaagagcaat cgaatgaaac tcccccatca
     3601 ggaaacattg acacgactgg aaggtatcaa tagcaaagtt tatcgaactg accagcaagg
     3661 agctatacgt tttaaggggt tggatagttg gaaaatcgaa agtgttcgat aggaaggata
     3721 aatgttgtag attagtgaaa taaactaaaa atttgttgca taataatgat aaaaacggca
     3781 taatgaaaac gtattcaata ttgaggatat aaaatcatta aaaatcagca aaagttgttt
     3841 tagttagtta gtttataatc tattggtctt cttcagtcca gtgtatctgc tgtgacagtc
     3901 actaaaagtt acaagtatga ttggaatacg gttttggaat atagtaccaa ctatcacgac
     3961 catcagtatg cttggattcc ggaatggtct cgttattaca gctattctga gtataaagtt
     4021 ggcggaggct ggaactacgc tcgttatgag gtcataaact actatactgg aagttattaa
     4081 tccttaaaga gtgagaaaag gagggctgga tatgttaaat cttactcatg ttaccttaaa
     4141 aacgcgacaa gtcatcttgc aagatgcgga ttttaccttt aaaaagggta ggatttatgg
     4201 ccttcttgct atcaatggct cgggaaagac gacactattc cgagctatga gcaagttgct
     4261 tccccttagt agtggacaca tcgcagttcc tccttctttg ttttattatg agagcgttga
     4321 atggctggat ggaaacttaa gtgggatgga ctaccttcgt ctcataaaaa acatctggaa
     4381 gtcagaccta aacttgagag atgaaatcgc ctactgggaa atgactgact atatcagtct
     4441 tcccatccgc aagtattcct tagggatgaa gcaacgcttg gtgattgcta tgtattttct
     4501 cagtcaggcc aaatgctggc tcatggatga gattacaaat ggcttagacg agtattatcg
     4561 acagaagttt tttgataggc tagcacaaat cgatagacaa gaacagctgg ttcttttaag
     4621 ttcccactat aaggaagagt tggttgatat ctgcgataga gtagtaacca ttcatcaggg
     4681 gcagatagaa gaggtttagt ttatgaaaga tgttagtcta tttttattga aaaaagtttt
     4741 caaaagtcgt ttaaactgga ttatcttagc tttatttgta tctgtactcg gtgttacctt
     4801 ttatttaaat agtcagactg caaactcaca cagcttggag agcaggttgg aaagtcgcat
     4861 tgcagccaac gagagggcta tcaatgaaaa tgaagagaaa ctctcccaaa tgtctgatac
     4921 cagctcggag gaataccagt ttgctaaaaa taatttagac gtgcaaaaaa atcttttgac
     4981 gcgaaagaca gaaattctaa ctttactaaa agaagggcgc tggaaagaag cctactatct
     5041 gcagtggaaa gatgaagaga agaattatga atttgtatca aatgacccga ctgctagctc
     5101 tggcttaaaa atgggggttg accgcgaacg gaagatttac caagccctgt atcccttgaa
     5161 cataaaagca catactttgg agtttccgac ccacgggatt gatcagattg tctggatttt
     5221 agaggttatc atcccaagct tgtttgtggt tgcgattatt tttatgctaa cacaactatt
     5281 tgcagaaaga tatcaaaatc atctggacac agctcactta tatcctgttt caaaagtgac
     5341 atttgcaata tcctctcttg gagttggagt gggatatgta actgtgctgt ttatcggaat
     5401 ctgtggcttt tcttttctag tgggaagtct gataagtggt tttggacagt tagattatcc
     5461 ctacccaatt tatagcttag tgaatcaaga agtaactatt gggaaaatac aagatgtatt
     5521 atttcctggc ttgctcttag ctttcttagc ctttatcgtc attgtggaag ttgtgtactt
     5581 gattgcttac tttttcaagc aaaaaatgcc tgtcctcttt ctttcactca ttgggattgt
     5641 tggcttattg tttggcatcc aaacaattca gcctcttcaa aggattgcac atctgattcc
     5701 ctttacttac ttgcgttcag tggagatttt atctggaaga ttacctaagc agattgataa
     5761 tgtcgatcta aattggagca tgggaatggt cttacttcct tgcctgatta tctttttgct
     5821 attgggaatt ctatttattg aaagatgggg aagttcacag aaaaaaagaa ttttttaata
     5881 gattctagct ttcctatagg tagggaaaat aagtaaaaac taacatagag agggaatcaa
     5941 cttgattctc tctttttgat tcgaaaacca aaccaaaata caaacacaaa cttttcaaaa
     6001 aataactttt tatcttgaca agagctagaa aacttggtat catataaaag ttgagaaaag
     6061 cagaagtgag agcttctcgc cttgtgacat taagttgcct ggccctacgg atgaaaagtt
     6121 tcgaagaaac gctatcataa cgtgcgggct tgtatattta cgagtccgct attgtttttc
     6181 tctaataaaa caaaagaggt gaaaaccata gcaaagcaag acttattcat caatgatgag
     6241 attcgtgtac gtgaagttcg cttgattggt cttgaaggag aacagctagg catcaagcca
     6301 ctcagtgaag cgcaagcttt ggctgataac gctaatgttg acctagtatt gattcaaccc
     6361 caagccaaac cgcctgttgc aaaaattatg gactacggta agttcaaatt tgagtaccag
     6421 aagaagcaaa aagaacaacg taaaaaacaa agcgttgtta ctgtgaaaga agttcgtcta
     6481 agtccaacta ttgacaaggg tgactttgat acaaaacttc gtaatgcacg taagttcctt
     6541 gaaaaaggaa ataaagttaa ggtatctatt cgctttaagg gtcgtatgat tacccataaa
     6601 gagattggtg caaaagtttt agccgagttt gctgaagcaa ctcaagatat tgccatcatc
     6661 gaacaacgtg ctaaaatgga tggacgtcaa atgttcatgc aattggcgcc agcgactgac
     6721 aaaaaataat tgtcagaaag ttaaataaag gagaaaaaat tatgccaaaa caaaaaacac
     6781 accgcgcatc agctaaacgt ttcaaacgta caggttctgg tggacttaaa cgtttccgtg
     6841 cttacacttc tcaccgtttc cacggaaaaa ctaagaaaca acgtcgtcat cttcgtaaag
     6901 catctatggt gcattcagga gattacaaac gtatcaaagc aatgcttact cgcttgaaat
     6961 aatagcttga ctgtaagtaa acaattctag gaaatatttg gaggaaataa aacatggcac
     7021 gtgttaaagg tggcgttgta tcacgcaaac gtcgtaaacg tattcttaaa ttagcaaaag
     7081 gttactatgg agctaaacac atcttgttcc gtactgcaaa agaacaagta atgaactctt
     7141 actactatgc ataccgtgac cgtcgtcaga aaaaacgtga cttccgcaaa ttgtggatca
     7201 ctcgtatcaa tgcggcagct cgtatgaacg gactttcata ctcacaattg atgcatggtt
     7261 tgaaattggc tgagatcgaa gttaaccgta aaatgcttgc tgacttggct gttaacgatg
     7321 cagtagcttt cacagctctt gcagatgcag ctaaagcaaa acttggtaaa taacaactga
     7381 tgagactgga acaggattgt ccagtctttt taaaaataaa ggagaaaaat atggcttcaa
     7441 aaatgctaca cacttgctta cgagtagaaa atcttgaaaa atcaatcgca ttttatcaag
     7501 atgcttttgg ttttaaagaa ttgcgtcgca gagattttcc agaccatgcc ttcacgattg
     7561 tctatctagg tcttgagggt gatgactatg agttggagtt gacttataac tacgatcacg
     7621 gtccgtatgt ggttggtgat gggtttgccc atatcgccct cagtacacct gatcttgagg
     7681 cacttcatca agaacacagt gcaaaaggct atgaagtgac tgagccaaat ggtctaccag
     7741 gaactacacc aaactattac tttgtcaaag accctgatgg atataaggtc gaagtcattc
     7801 gtgaaaaata atttcgtgca agttaagtag aatattcgtt ttttacttga ctttttttag
     7861 ctgaaagagt atactaataa aaattcaacc tttaagggga gtccagagag actcacaagg
     7921 tgtcagataa aagaatagtg caattttcta gaggagactt tttgagtgtg ctctcttgtg
     7981 ttgtacgatt ttaactgagg ccttgcacta gcaaggtctt ttctttatct ggtcccctta
     8041 aaatttaagg aggaaaagtt atgaatccca catgtaagaa gcgtttgggt gtcattcggt
     8101 tggaaaccat gaaggtggtt gcacaagagg aaatcgcgcc agcaatcttt gaattagtcc
     8161 tagaaggaga aatggttgaa gccatgcgag caggccaatt tcttcatctg cgtgtaccag
     8221 acgatgccca tcttttacgt cgtcctattt caatttcgtc tattgacaag gcaaacaagc
     8281 agtgtcacct catttatcgg attgagggag ctgggactgc aattttttca accttaagtc
     8341 agggagacac tcttgatgtg atggggcctc agggaaatgg ttttgacttg tctgaccttg
     8401 atgagcagaa tcaggttctc cttgttggtg gtgggattgg tgttccaccc ttgcttgagg
     8461 tggccaagga attgcatgaa cgtggagtga aagtagtgac agtcctcggt tttgctaata
     8521 aggatgctgt tattttgaaa acggaattgg ctcagtatgg tcaggtcttt gtaacgacag
     8581 atgatggttc ttatggcatc aagggaaatg tttccgttgt tatcaatgat ttagatagtc
     8641 agtttgatgc tgtttactcg tgtggggctc caggaatgat gaagtatatc aatcaaacct
     8701 ttgatgatca cccaagagcc tatttatctc tggaatctcg tatggcttgt gggatgggag
     8761 cttgctatgc ctgtgttcta aaagtaccag aaagcgagac ggtcaaccaa cgcgtctgtg
     8821 aagatggtcc tgttttccgc acaggaacag ttgtattata aggagaaaat tatgactaca
     8881 aatcgattac aagtgtctct acctggtttg gatttgaaaa atccgattat tccagcatca
     8941 ggttgttttg gctttggaca agagtatgcc aagtactatg atttaaacct tttaggttct
     9001 attatgatca aggcgacaac ccttgaacca cgttttggga acccaactcc aagagtagca
     9061 gagacgcctg ctggtatgct caatgcaatt ggcttgcaaa atcctggttt agaggttgtt
     9121 ttggctgaaa agctaccttg gctggaaaga gaatatccaa atcttcctat tattgccaat
     9181 gtagctggtt tttcaaaaca agagtatgca gctgtttctc atgggatttc caaggcaact
     9241 aatgtaaaag ctatcgagct caatatttct tgtcccaatg ttgaccactg taatcatgga
     9301 cttttgattg gtcaagatcc agatttggct tatgatgtgg tgaaagcagc tgtggaagcc
     9361 tcagaagtgc cagtttatgt caaattaacc ccgagtgtga ccgatatcgt tactgtcgca
     9421 aaagctgcag aagatgcggg agcaagtggc ttgaccatga tcaatactct ggttggaatg
     9481 cgctttgacc tcaaaaccag aaaaccaatc ttggccaatg gaacaggtgg aatgtcaggt
     9541 ccagcagtct ttccagtagc cctcaaactc atccgccaag tagcccaaac aacagacctg
     9601 cctatcattg gaatgggggg agtggattcg gctgaagctg ccctagaaat gtatctggct
     9661 ggggcatctg ctatcggagt tggaacagct aactttacca atccttatgc ctgccctgac
     9721 atcatcgaaa atttaccaaa agtcatggat aaatacggta ttagcagtct ggaagaactc
     9781 cgtcaggaag taaaagagtc tctgaggtaa actgcaatca atctgttctt gattttttat
     9841 tagtttgtaa tatgaattta ggagaatttt ggtacaataa aataaataag aacagaggaa
     9901 gaaggttaat gaagaaagta agatttattt ttttagctct gctatttttc ttagctagtc
     9961 cagagggtgc aatggctagt gatggtactt ggcaaggaaa acagtatctg aaagaagatg
    10021 gcagtcaagc agcgaatgag tgggtttttg atactcatta tcaatcttgg ttctatataa
    10081 aagcagatgc taactatgct gaaaatgaat ggctaaagca aggtgacgac tatttttacc
    10141 tcaaatctgg tggctatatg gccaaatcag aatgggtaga agacaaggga gccttttatt
    10201 atcttgacca agatggaaag atgaaaagaa atgcttgggt aggaacttcc tatgttggtg
    10261 caacaggtgc caaagtaata gaagactggg tctatgattc tcaatacgat gcttggtttt
    10321 atatcaaagc agatggacag cacgcagaga aagaatggct ccaaattaaa gggaaggact
    10381 attatttcaa atccggtggt tatctactaa caagtcagtg gattaatcaa gcttatgtga
    10441 atgctagtgg tgccaaagta cagcaaggtt ggctttttga caaacaatac caatcttggt
    10501 tttacataaa agaaaatgga aactatgctg ataaagaatg gattttcgag aatggtcact
    10561 attattatct aaaatctggt ggctacatgg cagccaatga atggatttgg gataaggaat
    10621 cttggtttta tctcaaattt gatgggaaaa tagctgaaaa agaatgggtc tacgattctc
    10681 atagtcaagc ctggtactac ttcaaatccg gtggttacat gacagccaat gaatggattt
    10741 gggataagga atcttggttt tacctcaagt ctgatggcaa aatggctgaa aaagaatggg
    10801 tctacgattc tcatagtcaa gcctggtact acttcaaatc tggtggttac atggcgaaaa
    10861 atgagacagt agatggttat cagcttggaa gcgatggtaa atggcttgga ggaaaagcta
    10921 caaatgaaaa tgctgcttac tatcaagtag tgcctgttac agccaatgtt tatgattcag
    10981 atggtgaaaa gctttcctat atatcgcaag gtagtgtcgt atggctagat aaggatagaa
    11041 aaagtgatga caagcgcttg gctattacta tttctggttt gtcaggctat atgaaaacag
    11101 aagatttaca agcgctagat gctagtaagg actttatccc ttattatgag agtgatggcc
    11161 accgttttta tcactatgtg gctcagaatg ctagtatccc agtagcttct catctttctg
    11221 atatggaagt aggcaagaaa tattattcgg cagatggcct gcattttgat ggttttaagc
    11281 ttgagaatcc cttccttttc aaagatttaa cagaggctac aaactacagt gctgaagaat
    11341 tggataaggt atttagtttg ctaaacatta acaatagcct tttggagaac aagggcgcta
    11401 cttttaagga agccgaagaa cattaccata tcaatgctct ttatctcctt gcccatagtg
    11461 ccctagaaag taactgggga agaagtaaaa ttgccaaaga taagaataat ttctttggca
    11521 ttacagccta tgatacgacc ccttaccttt ctgctaagac atttgatgat gtggataagg
    11581 gaattttagg tgcaaccaag tggattaagg aaaattatat cgatagggga agaactttcc
    11641 ttggaaacaa ggcttctggt atgaatgtgg aatatgcttc agacccttat tggggcgaaa
    11701 aaattgctag tgtgatgatg aaaatcaatg agaagctagg tggcaaagat tagtactata
    11761 agtgaatatg atttgagtga atagtaagtt aaaaatcctg atttcaagta aaatcaggat
    11821 tttttcatgg atgcaatttt tttggagtct ggtgtgacgc ggagggtctt ttgtcctgtg
    11881 taagtgacaa agccgggttt tccaccagtt ggtttattga gttttttgac ttcaatcata
    11941 tctacctgca ccagattcga caggcgccct tgagagaagt aggcagctaa ctctgctgcg
    12001 tctgtcttga ctgcatcaga tgggtcaaga tttcctgaga tgacaacatg gcttccagga
    12061 atgtccttag catggaacca aagttcctcc ttgcgggcca ttttaaaggt caattcctca
    12121 ttttgaagat tgtttcgtcc gacatagatg atggttttgc catcgcttgc tagatattgt
    12181 tctagttttt tgcgtttctg gattttctcc cgttgtcttc tgcggataaa acctgtttga
    12241 atcaattctt cacggatttc agcgatttct tccagtccag cttggttgag gacggtttct
    12301 acactttcca gatagagaat agtggctttg gtttcttcaa tcaaatcagt caagtatttg
    12361 acagcttctt tgagtttctg ataccgttta aaatagcgtt gggcattctg gttgggagtc
    12421 agagccttat caagcgcaat catgataggt tggttggtat agtagttgtc taggataacc
    12481 tggtcttggt cgttaggcac ttggtggagg aaggttgtca gcaattctcc tttttgacga
    12541 aattcttcag cgttgtctgt cgccagtaac tctttttcct gttttttgag tttgtgtcgg
    12601 tttttctgaa gttcattttc aacacgacga atcagttcac tggcctgctg tttgacgcgg
    12661 tcgcgctcag ccttatcctt atagtaggtg tccaacaaat cagaaagatt tgcaagaggc
    12721 tctcccacct gatttgcaaa aggaactgga ctgaaggaag tctcagtcaa gcatggcttg
    12781 gtttcttgat tgaaaaaatt tcggaaagcg gaaagttttt cactaaccag tatcctttcc
    12841 aattcatttg ccgtatcgcg tcccagacct tgaaagaggc tttgaagatt ttttgctgtt
    12901 agttcttggg tttgcaggat ttcaaagagc ttttcatcct tgatagtaaa aggattgaga
    12961 gattttgtac ttggcggagc gatataggtc gatcctggaa gtaaggtgcg gtagctattt
    13021 tgtgaaaagc cgacgtgttt gataacttcg aggattttat gactgctttt atcgaccagt
    13081 agaatattac tgtgtttccc cataatttcg ataatcaagg tagcctggat atggtctcca
    13141 atctcgtttt tattggaaac tgtaatttcc acaatacggt cattttccac ttgctcaatc
    13201 gactcaatca gggccccctg caaatacttt ctcaaaacca tgataaaggt agaaggttgg
    13261 gctggatttt caaaagtcgt ttgggtcagc tgaatgcgtc caaaaactgg atgggcagaa
    13321 aggagcaggc gatggctttg gcgattgctg cggatttgca agaccaactc ttgttcaaaa
    13381 ggctgattga ttttctggat gcgaccattc actaattcgc ttcgcaattc ctcaactatg
    13441 tggtgtaaaa aaaatccgtc aaatgacatc gttctctcct tgtgattgta ttccatagta
    13501 ttatatcaaa aaggtagaat aaaatcatgg aaatgtggta taataaagcc aagtaaagag
    13561 aaacgagaag cacatgtata ttgaaatggt agatgaaact ggtcaagttt caaaagaaat
    13621 gttgcaacaa acccaagaaa ttttggaatt tgcagcccaa aaattaggaa aagaagacaa
    13681 ggagatggca gtcacttttg tgaccaatga gcgtagtcat gaacttaatc tggggtaccg
    13741 tgacaccgac cgtccgacag atgtcatcag ccttgagtat aaaccagaat tggaaattgc
    13801 ctttgacgaa gaggatttgc ttgaaaatcc agaattggca gagatgatgt ctgagtttga
    13861 tgcctatatt ggggaattgt tcatctctat cgataaggct catgagcagg ccgaagaata
    13921 tggtcacagc tttgagcgtg agatgggctt cttggcagta cacggctttt tacatattaa
    13981 cggctatgat cactatactc cggaagaaga agcggagatg ttcggtttac aagaagaaat
    14041 tttgacagcc tatggactca caagacaata aacgaaaatg gaaaaatcgt gacctgatat
    14101 ccagtttaga atttgccatc acagggattt ttactgctat caaggaagaa cgcaatatgc
    14161 gaaaacatgc agtgacggct ctagtggtca tccttgcagg ttttggtttt caggtgtcac
    14221 gaatcgaatg gctctttctc ctattgagca ttttcttggt ggtagccttt gaaattatca
    14281 attctgctat cgaaaatgtg gtggatttgg ccagtcacta tcacttttcc atgctggcta
    14341 aaaatgccaa ggatatggcg gctggcgcgg tattagtggt ttctcttttt gcagccttaa
    14401 caggcgcatt gatttttctc ccacgaatct gggatttatt attttaaaca gtaagaggaa
    14461 attatgactt ttaaatcagg ctttgtagcc attttaggac gtcccaatgt tgggaagtca
    14521 acctttttaa atcacgttat ggggcaaaag attgccatca tgagtgacaa ggcgcagaca
    14581 acgcgcaata aaatcatggg aatttacacg actgataagg agcaaattgt ctttatcgac
    14641 acaccaggga ttcacaaacc taaaacagct ctcggagatt tcatggttga gtctgcctac
    14701 agtacccttc gcgaagtgga cactgttctt ttcatggtgc ctgctgatga agcgcgtggt
    14761 aagggggacg atatgattat cgagcgtctc aaggctgcca aggttcctgt gattttggtg
    14821 gtgaataaaa tcgataaggt ccatccagac cagctcttgt ctcagattga tgacttccgt
    14881 aatcaaatgg actttaagga aattgttcca atctcagccc ttcagggaaa taacgtgtct
    14941 cgtctagtgg atattttgag tgaaaatctg gatgaaggtt ttcaatattt cccgtctgat
    15001 caaatcacag accatccaga acgtttctta gtttcagaaa tggttcgcga gaaagtcttg
    15061 cacctaactc gtgaagagat tccgcattct gtagcagtag ttgttgactc tatgaaacga
    15121 gacgaagaga cagacaaggt tcacatccgt gcaaccatca tggtcgagcg cgatagccaa
    15181 aaagggatta tcatcggtaa aggtggcgct atgcttaaga aaatcggtag catggcccgt
    15241 cgtgatatcg aactcatgct aggagacaag gtcttcctag aaacctgggt caaggtcaag
    15301 aaaaactggc gcgataaaaa gctagatttg gctgactttg gctataatga aagagaatac
    15361 taagtagagg tgggctcatg cctgcttctt gtttttacag aaggaggact tatgcctgaa
    15421 ttacctgagg ttgaaaccgt ttgtcgttgc ttagaaaaat tgattatagg aaagaagatt
    15481 tcgagtatag aaattcgcta ccccaagatg attaagacgg atttggaaga gtttcaaagg
    15541 gaattgccta gtcagattat cgagtcaatg ggacgtcgtg gaaaatattt gcttttttat
    15601 ctgacagaca aggttttgat ttcccatttg cggatggagg gcaagtattt ttactatcca
    15661 gaccaaggac ctgaacgcaa gcatgcccat gttttctttc attttgaaga tggtggcacg
    15721 cttgtttatg aggatgttcg caagtttgga accatggaac tcttggtgcc tgacctttta
    15781 gacgcctact ttatttctaa aaaattaggt cctgaaccaa gcgaacaaga ctttgattta
    15841 caggtctttc aatctgccct tgccaagtcc aaaaagccta tcaaatccca tctcctagac
    15901 cagaccttgg tagctggact tggcaatatc tatgtggatg aggttctctg gcgagctcag
    15961 gttcatccag ctagatcttc ccagactttg acagcagaag aagcgacagc tattcatgac
    16021 caaaccattg ctgttttgag ccaggctgtt gaaaaaggcg gttccaccat tcggacctat
    16081 accaatgcct ttggggaaga tggaagcatg caggattttc atcaggtcta tgataagact
    16141 ggtcaaggat gtgtacgctg tggtaccatc attgagaaaa ttcaactagg tggacgtgga
    16201 acccactttt gtccaaactg tcaaaggagg gactgatggg aaaaatcatc ggaatcactg
    16261 ggggaattgc ctctggtaag tcaactgtga caaattttct aagacagcaa ggctttcaag
    16321 tagtggatgc cgacgcagtc gtccaccaac tacagaaacc tggtggtcgt ctgtttgagg
    16381 ctctagtaca gcacttttgg caagaaatca ttcttgaaaa cggagaactc aatcgccctc
    16441 tcctagctag tctcatcttt tcaaatcctg atgaacgaga atggtctaag caaattcaag
    16501 gggagattat ccgtgaggaa ctggctactt tgagagaaca gttggctcag acagaagaga
    16561 ttttcttcat ggatattccc ctactttttg agcaggacta cagcgattgg tttgctgaga
    16621 cttggttggt ctatgtggac cgagatgccc aagtagaacg cttaatgaaa agggaccagt
    16681 tgtccaaaga tgaagctgag tctcgtctgg cagcccagtg gcctttagaa aaaaagaaag
    16741 atttggccag ccaggttctt gataataatg gcaatcagaa ccagcttctt aatcaagtgc
    16801 atatccttct tgagggaggt aggcaagatg acagagatta actggaagga taatctgcgc
    16861 attgcctggt ttggtaattt tctgacagga gccagtattt ctttggttgt accttttatg
    16921 cccatcttcg tggaaaatct aggtgtaggg agtgagcaag tcgcttttta tgcaggctta
    16981 gcaatttctg tctctgctat ttccgcggcg ctcttttctc ctatttgggg tattcttgct
    17041 gacaaatacg gccgaaaacc catgatgatt cgggcaggtc ttgctatgac tatcactatg
    17101 ggaggcttgg cctttgtccc aaatatctat tggttaatct ttcttcgttt actaaacggt
    17161 gtatttgcag gttttgttcc taatgcaacg gcactgatag ccagtcaggt tccaaaggag
    17221 aaatcaggct ctgccttagg tactttgtct acaggcgtag ttgcaggtac tctaactggt
    17281 ccctttattg gtggctttat cgcagaatta tttggcattc gtacagtttt cttactggtt
    17341 ggtagttttc tatttttagc tgctattttg actatttgct ttatcaagga agattttcaa
    17401 ccagtagcca aggaaaaggc tattccaaca aaggaattat ttacctcggt taaatatccc
    17461 tatcttttgc tcaatctctt tttaaccagt tttgtcatcc aattttcagc tcaatcgatt
    17521 ggccctattt tggctcttta tgtacgcgat ttagggcaga cagagaatct tctttttgtc
    17581 tctggtttga ttgtgtccag tatgggcttt tccagcatga tgagtgcagg agtcatgggc
    17641 aagctaggtg acaaggtggg caatcatcgt ctcttggttg tcgcccagtt ttattcagtc
    17701 atcatctatc tcctctgtgc caatgcctct agcccccttc aactaggact ctatcgtttc
    17761 ctctttggat tgggaaccgg tgccttgatt cccggggtta atgccctact cagcaaaatg
    17821 actcccaaag ccggcatttc gagggtcttt gccttcaatc aggtattctt ttatctggga
    17881 ggtgttgttg gtcccatggc aggttctgca gtagcaggtc aatttggcta ccatgctgtc
    17941 ttttatgcga cgagcctttg tgttgccttt agttgtctct ttaacctgat tcaatttcga
    18001 acattattaa aagtaaagga aatctagtgc gagtaaaaat taatctcaaa tgctcctctt
    18061 gtggcagtat caattaccta accagtaaaa attcaaaaac ccatccagac aagattgagg
    18121 ttttaaagta ttgtcccaag gaaagaaaag taaccctaca tcttgaatct aagtagattt
    18181 tatggtaaaa taatagggat tttaaggagt ttgatatgta taacctatta ttaaccattt
    18241 tattagtatt atctgttgtg attgtgattg caattttcat gcaaccaacc aaaaaccaat
    18301 ccagcaatgt atttgatgcc agttcaggtg atttgtttga acgtagtaaa gttcgcggtt
    18361 ttgaagctgt aatgcagcgt ttgacaggga ttttagtctt tttctggcta gccattgcct
    18421 tagcattgac ggtattatca agtagataag aaaataatgg gcaggactag gtctttgcct
    18481 ctttttattt ttaaaggatg tttgagaagg ttttacagta aaagaaaatt aaaaaatcta
    18541 gaaagaaaat atgaaagata gaataaaaga atatttacaa gacaagggaa aggtgactgt
    18601 taatgatttg gctcaggctt tgggaaaaga cagttccaag gattttcgtg agttgattaa
    18661 aaccttgtcc ttaatggaaa gaaagcacca aattcgtttt gaagaagatg gtagtctgac
    18721 attagaaatt aagaaaaaac atgagattac cctcaagggg atttttcatg cccataaaaa
    18781 tggctttggc tttgttagtc tggaaggcga ggaggacgac ctttttgtag ggaaaaatga
    18841 tgtcaactat gctattgatg gtgataccgt cgaggtagtg attaagaaag tcgctgaccg
    18901 caataaggga acagcagcag aagccaaaat tattgatatc ctagaacaca gtttgacaac
    18961 agttgtcggg caaatcgttc tggatcagga aaaacctaag tatgctggct atattcgttc
    19021 aaaaaatcag aaaatcagtc aaccgattta tgttaagaaa ccagccctaa aattagaagg
    19081 aacagaagtt ctcaaagtct ttatcgataa atacccaagc aagaaacatg atttctttgt
    19141 cgcgagtgtt ctcgatgtag tgggacactc aacggatgtc ggaattgatg ttcttgaggt
    19201 cttggaatca atggacattg tatccgagtt tccagaagct gttgttaagg aagcagaaag
    19261 tgtgcctgat gctccgtctc aaaaggatat ggaaggtcgt ctggatctaa gagatgaaat
    19321 tacctttacc attgacggtg cggatgccaa ggacttggac gatgcagtgc atatcaaggc
    19381 tctgaaaaat ggcaatctgg agcttggggt tcacatcgca gatgtttctt attatgtgac
    19441 cgaggggtct gcccttgaca aggaagccct taaccgtgcg acttctgttt acgtgacaga
    19501 ccgagtggtg ccaatgcttc cagaacgact atcaaatggc atctgctctc tcaatcccca
    19561 agttgaccgc ctgacccagt ctgctattat ggagattgat aaacatggtc gtgtggtcaa
    19621 ctataccatt acacaaacag ttatcaagac cagttttcgt atgacctata gcgatgtcaa
    19681 tgatatccta gctggcgatg aagaaaagag aaaagaatat cataaaattg tatcaagtat
    19741 cgaactcatg gccaagcttc atgaaacttt agaaaacatg cgtgtgaaac gtggagctct
    19801 caattttgat accaatgaag cgaagatttt agtggataaa caaggtaagc ctgttgatat
    19861 cgttcttcgg cagcgtggta ttgccgagcg gatgattgag tcttttatgt tgatggctaa
    19921 tgaaacagtt gccgaacatt tcagcaagtt ggatttgcct tttatctatc gaattcacga
    19981 ggagcctaag gctgaaaagg ttcagaagtt tattgattat gcttcgagtt ttggcttgcg
    20041 catttatgga actgccagtg agattagtca ggaggcactt caagacatca tgcgtgctgt
    20101 tgagggagaa ccttatgcag atgtattgtc catgatgctt cttcgctcta tgcagcaggc
    20161 tcgttattcg gagcacaatc acggccacta tggactagct gctaactatt atactcactt
    20221 taccagtcca attcgtcgtt atccagacct tcttgttcac cgtatgattc gggattacgg
    20281 ccgttctaag gaaatagcag agcattttga acaagtgatt ccagagattg cgacccagtc
    20341 ttccaaccgt gaacgtcgtg ccatagaagc tgagcgtgaa gtcgaagcca tgaaaaaggc
    20401 tgagtatatg gaagaatacg tgggtgaaga gtatgatgca gttgtatcaa gtattgtcaa
    20461 attcggtctc tttgtcgaat tgccaaacac agttgaaggc ttgattcaca tcactaatct
    20521 acctgaattt tatcatttca atgagcgtga tttgactctt cgtggagaaa aatcaggtat
    20581 cactttccga gtgggtcagc agatccgtat ccgtgttgaa agagcggata aaatgactgg
    20641 agagattgat ttttcattcg tacctagtga gtttgatgtg attgaaaaag gcttgaaaca
    20701 gtctagtcat agtggcagag ggcgtggttc aaatcgtcgt tcggataaga aggaagacaa
    20761 gagaaaatca ggacgctcaa atgataagcg taagcattca caaaaagaca agaagaaaaa
    20821 aggaaagaaa cctttttaca aggaagtagc taagaaagga gccaagcatg gcaaagggcg
    20881 agggaaaggt cgtcgcacaa aataaaaagg cacgccacga ctatacaatc gtagatacgc
    20941 tagaggcagg gatggtcctg actggaactg aaatcaagag tgtacgagct gctcgaatta
    21001 atctcaagga tggctttgct caagtgaaaa atggagaagt ttggctgagc aatgttcata
    21061 tcgcgcctta cgaagagggc aatatctgga accaggaacc agaacgtcgt cgtaaactcc
    21121 tgctccataa aaagcaaatt caaaaattgg aacaagagac caaagggaca ggaatgacct
    21181 tagttcccct taaggtctat ataaaagatg gctacgctaa gcttctttta ggacttgcca
    21241 aagggaagca tgactatgac aaacgggagt ctatcaaacg tcgtgagcaa aatcgagata
    21301 tcgcgcgtgt gatgaaagct gttaatcagc gataaaaaga ggaattgaaa atggaaaaat
    21361 tagttgccta taaacgcatg cctttgtgga ataaacaaac aatgcctgaa gctgttcagc
    21421 aaaagcacaa tacaaaagtt gggacttggg ggaaaattac tgtcttgaag ggagctctca
    21481 agtttattga attgacagaa gaaggggaag ttctagctga acacctcttt gaagcagggg
    21541 cagacaatcc aatggcccaa cctcaagcct ggcaccgagt ggaagctgcc acagatgatg
    21601 tggaatggta cttggaattt tattgtaaac ctgaggatta ttttgctaaa aaatacaata
    21661 ccaatcctgt tcattcagag gtcctagagg ccatgcagac agtgaaacaa gggaaagctt
    21721 tggatttggg ttgtggtcag gggcgtaatt ctctttttct agcccagcaa gattttgatg
    21781 tgacggctgt agatcaaaat ggactagctc ttgaaatctt gcaaagcatt gtggagcagg
    21841 aagatttgga catgcctgtt ggcctttacg atatcaattc agctagcatt gaacaagaat
    21901 atgattttat cgtttcaaca gttgttctca tgtttctaca agcggaccgc attccagcta
    21961 ttattcaaaa tatgcaggag aaaaccagtg ttggtggtta caaccttatc gtttgtgcca
    22021 tggacacgga ggattatcct tgctcggtta atttcccatt cacctttaaa gaaggagaac
    22081 tggcagacta ttacaaggat tgggaattgg ttaagtacaa tgaaaatcca ggccatttgc
    22141 accgtcgcga tgagaatggc aatcgtattc aactacgctt tgcgacctta ctagctaaga
    22201 aaatcaagta aacacacatg aagattagga attttcctga tcttttttct tttttacgaa
    22261 tgatatagaa aaggagggaa ttcatgtttg ttgcgagaga tgctagggga gaattggtaa
    22321 atgtgttaga ggataaactt gagaagcaag catacacctg cccagcttgt ggaggccagc
    22381 tccatttgcg tcaaggacca agtgtacgga cgcattttgc ccataaatcc ttaaaagact
    22441 gtgatttttt ctttgaaaat gaaagtccag aacacctggc caataaggaa tccctctatc
    22501 actggttgaa aaaagagaca aaggttcaat tagagtaccc gctttcagaa cttaaacaga
    22561 ttgcggatgt atttgtaaat ggcaatctag ctctagaagt tcagtgtagt cccttgcctc
    22621 agaaagtcct taaagagcga agtgagggat atcgtagtca gggttaccaa gtactgtggt
    22681 tgctgggtca aaaactgtgg ctcaaggagc gtttgactcg tctacagcaa ggttttcttt
    22741 atttcagtca aaacatgggc ttttatgttt gggaattaga caaggaaaaa caagttttaa
    22801 gactcaaata cctgatttac caggatctcc gcggtaaact ccattatcaa atcaaggaat
    22861 tttcctatgg tcaaggtagt ttattggaaa tattgcgtct tccctataag aaacaaaaaa
    22921 tatctcattt tacagtttct gaggacaagg acatctgtcg ctatatccgg caacaacttt
    22981 attatcaaaa tctcttttgg atgaaagaac aagcagaagc ctatcaaaag ggagaaaata
    23041 tcctgactta tggactgaaa gaatggtatc cacaaattcg accaatagtg ggcaaatttt
    23101 tccagattga acaagacttg actagctatt atcagcactt ttatacctat taccaaaaaa
    23161 atcctcaaaa tgattggcaa aagctttatc caccagcctt ttatcagcaa tatttcttga
    23221 aaaatatggt agaatagaaa ggatggagga atctaatggt attacaaaga aatgaaataa
    23281 atgaaaaaga tacatgggat ctatcaacga tctacccaac tgaccaggct tgggaagaag
    23341 tcttaaaaga tttaacagaa caattggaga cagtagccca gtatgaaggc catctcttgg
    23401 atagtgcgga taacctacta gaaatcactg aattttctct tgaaatggaa cgccagatgg
    23461 agaagcttta cgtttatgct catatgaaga atgaccagga tacacgtgaa gctaagtatc
    23521 aagagtacta tgccaaggcc atgacattct acagccagtt agaccaagcc ttttcattct
    23581 atgagcctga atttatggag attagcgaaa agcagtatgc tgacttttta gaagctcaac
    23641 caaagctgca ggtttatcaa cactattttg acaagctctt gcaaggcaag gatcacgttc
    23701 tctcacaacg tgaagaagaa ttattggctg gagctggaga aatctttggt tcagcaagtg
    23761 aaaccttcgc tatcttggac aatgcggata ttgtgttccc ttatgtccta gacgatgatg
    23821 gtaaagaagt tcagctatct catgggattt acacacgttt gatggagtct aaaaaacgtg
    23881 aggttcgccg tggtgcctat caagctcttt atgcgactta cgaacaattc caacacacct
    23941 atgccaaaac cttgcaaacc aatgttaagg tgcaaaatta ccgtgctaaa gttcgtaact
    24001 acaagagtgc tcgtcatgca gccctcgcag cgaattttgt tccagaaagt gtttatgaca
    24061 atttggtagc agcagttcgc aagcatttgc cactcttaca tcgctatctt gagcttcgtt
    24121 caaaaatctt ggggatttca gatctcaaga tgtacgatgt ctacacaccg ctttcatctg
    24181 ttgaatacag ttttacctac caagaagcct tgaaaaaagc agaagatgct ttggcagtct
    24241 tgggtgagga ttacttgagc cgtgttaaac gtgccttcag cgagcgttgg attgatgttt
    24301 acgaaaatca aggcaagcgt tcaggtgcct actctggtgg ttcttacgat accaatgcct
    24361 ttatgcttct caactggcag gacaatctgg acaatctctt tactcttgtt catgaaacag
    24421 gtcatagtat gcattcaagc tatactcgtg aaactcagcc ttatgtttac ggagattact
    24481 ctatcttttt ggctgagatt gcctcaacta ccaatgaaaa tatcttgacg gagaaattat
    24541 tggaagaagt ggaagacgac gcaacacgct ttgctattct caataacttc ctagatggtt
    24601 tccgtggaac agttttccgc caaactcaat ttgctgagtt tgaacacgcc attcaccaag
    24661 cagatcaaaa tggggaggtc ttgacaagcg atttcctaaa taaactctac gcagacttga
    24721 accaagagta ttatggtttg agtaaggaag acaatcctga aatccaatac gagtgggctc
    24781 gcattccaca cttctactat aactactatg tatatcaata ttcaactggc tttgcggccg
    24841 cctcagcctt ggctgaaaaa attgtccatg gtagtcaaga agaccgtgac cgctatatcg
    24901 actacctcaa ggcaggtaag tcggactatc cacttaatgt catgagaaaa gctggtgttg
    24961 atatggagaa ggaagactac ctcaacgatg cctttgcagt ctttgaacgc cgtttaaatg
    25021 agtttgaagc ccttgttgaa aaattaggat tggcataaaa tggttgaatc gtatagtaag
    25081 aatgctaacc ataacatgcg tcgtcctgtc gtcaaagaag aaattgtaga cttgatgcgt
    25141 cagcgtcaaa agcaggtcac aggtttcttg aaagaattgg aaaactttgc ccgcaaggaa
    25201 aatattccta ttattcccca tgaaacggtt gcttatttcc gttttcttat ggaaaccatg
    25261 cagcctaaaa atattctgga aattgggacg gctatcggtt tttcagctct cttgatggct
    25321 gaacatgcgc caaatgctaa gattacaact attgatcgta atccagaaat gattggtttt
    25381 gccaaggaaa attttgccca gtttgacagt cgcaagcaaa tcactctcct agagggagat
    25441 gcggtggatg tcttatctac actgacagag tcttatgatt tcgtctttat ggattctgcc
    25501 aagtctaaat acatcgtctt tctgccagaa atcctcaaac atttggaagt tggtggtgtg
    25561 gttgtcttgg atgatatttt tcaaggtggt gatgttgcca aggatattat ggaagtccgt
    25621 cgtggtcagc gaaccattta tcgaggcctt caaaaattat ttgatgcaac cttagacaat
    25681 ccagaactca ccgcaacatt agtgccttta ggagatggta ttctcatgct tcgtaaaaat
    25741 gtagcagatg ttcaactgtc tgaaagcgaa tgattttcag aaaaatttaa gaaaaaatag
    25801 taaaatagat agagtaacac ttatctcaaa ggagtagaca tgaagaaaaa attattggca
    25861 ggtgccatca cactattatc agtagcaact ttagcagctt gttcgaaagg gtcagaaggg
    25921 gcagacctta tcagcatgaa aggggatgtc atcacagaac atcaatttta tgagcaagtg
    25981 aaaaacaacc cttcagccca acaagtcttg ttaaatatga ccatccaaaa agtttttaaa
    26041 aaacaatatg gctcagagct tgatgataaa gaggttgatg atactattgc cgaagaaaaa
    26101 aaacaatatg gcgaaaacta ccaacgtgtc ttgtcacaag caggtatgac tcttgaaaca
    26161 cgtaaagctc aaattcgtac aagtaaatta gttgagttgg cagttaagaa ggtagcagaa
    26221 gctgaattga cagatgaagc ctataagaaa gcctttgatg agtacactcc agatgtaacg
    26281 gctcaaatca tccgtcttaa taatgaagat aaggccaaag aagttctcga aaaagccaag
    26341 gcagaaggtg ctgattttgc tcaattagcc aaagataatt caactgatga aaaaacaaaa
    26401 gaaaatggtg gagaaattac ctttgattct gcttcaacag aagtacctga gcaagtcaaa
    26461 aaagccgctt tcgctttaga tgtggatggt gtttctgatg tgattacagc aactggcaca
    26521 caagcctaca gtagccaata ttacattgta aaactcacta agaaaacaga aaaatcatct
    26581 aatattgatg actacaaaga aaaattaaaa actgttatct tgactcaaaa acaaaatgat
    26641 tcaacatttg ttcaaagcat tatcggaaaa gaattgcaag cagccaatat caaggttaag
    26701 gaccaagcct tccaaaatat ctttacccaa tatatcggtg gtggagattc aagctcaagc
    26761 agtagtacat caaacgaata gtccaaatca atgagtcagg gaaaaaaact cgacttcagg
    26821 aaaaaatgaa gcaaacattc ccacaataaa acgcatagta caaggtttgt actgacccca
    26881 aaagttagac aattaattta tctgaaggat ttagttctgt attgcacagg gctaagtcct
    26941 tttagtttta ccttaattct cttattgttg taataatcaa tatagtctat aatggcttgt
    27001 tccaattgat taagtgattt aaatgttttc tcatagccat aaaacatttc ggattttaaa
    27061 atgccaaaga aagattccat cctaccgttg tcttggctgt ttcctttgcg tgacatagat
    27121 gcttgaattc ccttactctc taggaagcga tgataagaat cgtgttggta ttgccagcct
    27181 tggtcactat ggagaatcgt attctcgtag tgcttctctt tgaatgcctg ttccaacatt
    27241 aacgatcaat caatttaatc atgtacctaa tattagaatt gtttatccca aatttatttg
    27301 aaagcttctc taagctatat ccttgttttc taagttcata gatctgaact ttatcatcat
    27361 aagttaattt cataataaaa acaccccaaa agttagattt tttctgtcta acttttgggg
    27421 tgtagttcat gtacacctga tatgatgcgt tttataattt taaagacttt ttgaccagcc
    27481 tcattttttt aacttgatac tcagtgaaaa gcaaagatta aactaggaag ctagctgtag
    27541 gctgctcaaa gaacagcttt gaggttgcag ataaaacttg tgaggtcacc aacatatata
    27601 atgtgaagct gacgtggctt gaatagattt tagaagggta tgagtctgga agttttaatg
    27661 gataatgcaa gattccatag aatgggtaag ctagagttct tatgtgaaga gtttgggcat
    27721 aaacttttac cttttcctcc ctactcatct tagtatagaa aagtgaatct aaaatagtac
    27781 ataactgctt ctaaaacatt cttataaatt gatttaaatt ctcaaatcat attattcagt
    27841 tcttatttca ttttgctcta caatcctgtt gagaagacac gtgttcatat caaaaaggta
    27901 ttggcaagtt gcaatacctt tttacaaggt tcttttgtct tatttttgtt tcaactgact
    27961 atatctccta tggttctagt tcagaaggct aggctataat tatgattgat aagaagtatc
    28021 attccaagta ttgagagtga atgtttcaaa atcatgggtt tctataatgg tcaggctggc
    28081 atttgctaga ccgccatctt tacgaagaag tggttcttta tagcctagga gagtacgaag
    28141 actggcagta agattggcgc cgtgtccgac aattagaata cgctcagctg gactatcttt
    28201 taatgatttg ataaattgga tggtccgttg agttgtacta tagagggatt cggctccgaa
    28261 cattcgagtg tcaaattgag caagatttga acgaaaagcc tggatttgtt gcgggtaaat
    28321 agcttccaag gttgcaattt tcaaaccttc taacttccca agttgccatt cacggagatt
    28381 aggaacgatt tctaaagaac agggggtata gagttgactt tggataatct cagcagattt
    28441 gaccgctcga ggtaaatcac ttgaataaat ctgatcaaaa ggaatttcct tgagatactg
    28501 gccaagtcgt tttagggttt caatggattc aggaagaaga ggagaatcac cgctagcacc
    28561 ttgaaaacga ccttcttggt tccagagggt acgaccgtgg cggacaaagt agagtttcat
    28621 tacttgatgt cctccaaaat atctacaaag tctgccttta caaagctagc caagtcttgt
    28681 ggcgcgacga taatgctgtg tccgacttcg cctgcagaga caatcattcg atccaaatct
    28741 agagcaattt tatcgataaa aatgggataa ttgtgtttct gacgaattcc gacaggatta
    28801 ttggctccat gaatgtaacc agttgttttt tctaagtcct tttgtggaat catgctcact
    28861 tttttattgc cagaaatttt agctagtttc ttttcagaca agtgctgagt gatagggaca
    28921 attccgataa tcggtccggt cttgtctccc aaaagcgcca aggttttgaa aatctgatct
    28981 cgttcataac cttgaggaag ctctccttct agggcattga tttgaatccc ctgatgaggg
    29041 atagctgctt tagataggat ttgttccacc aatgtttttt tgattttaac tttttttgcc
    29101 attatttata tttatcctcc aattgactca tccaaatacc aagccagatt cccagcgcaa
    29161 agaagaaggc gatgatgaca taaccgacaa gtgaaagtcc tgtgtattgg atactttcag
    29221 cgtttcctgc atttggaatt aagatcaaaa gggtacttga taggacgata ccgatgatga
    29281 aatgatagac gcgtgagtgg tagttgttta aggcataatc catcaatttt gaaaaaacga
    29341 tgagagttgc acctgcacca attccaatcg gaaagaaggt tcccaagaga tcaaaagttt
    29401 taaaaccagt caacatagga gcatagagtc ccaaaatcaa aagtaagttc gatgggctga
    29461 ggccaggaac caagacgcca agggccaata gtgcgcctgc taggacgaag ttaagaaagc
    29521 tggcgcttaa ggttccaacg acaaaattta aggcatagag tcctaatcca gaaatgataa
    29581 aggttgtcca taaccaagct aaatcaatct tgtctcggtc agattctcga gttgattctt
    29641 tgaggaggct aggaactgta ccgataatag ctcccgcaaa gctccataat acaaagacct
    29701 gataattttc aagcaggtat tcaatcgggt aggaaaataa gccgattccc agaagcatac
    29761 cgatggcaac tggaataaag tacaaaacat tttctttaag gtctttaaag ggatgggcca
    29821 gaaagccaat cattcgttca tagattccta agattgctgc tagaacccct ccggaaattc
    29881 ccggtaggat aaatccaaga gcaattacaa tccctttaat aacgcgtgct aaccatgaga
    29941 gcatatattt cctccaacta tttctatttc aaaaaatcct tactatattg tatcacaatc
    30001 agacacaaaa agaaaaagca aatgataaac aaatgctttt aaggttttaa aaaaagtttt
    30061 cgaaaggttt cttctttatt ttttaaggga gagataacgt tgatatctaa atcgtggtca
    30121 aagccggcaa tttttccttt agatgtgtat tggtgaatat cataatctaa atcagtttta
    30181 ggactgctct ccaaaaatcc tgagtctgag ccgtaggacg gaatccaaac agaggtaaac
    30241 ttgcctgtat caatactgtg ttcttccatg aagtagacac caacgtagat gccgatgttt
    30301 ttagcaccta gtgatgctag ttttgctcga aagttttcga caccttcgtt catattagac
    30361 atggttttgt cttccacgtc aagccaatag taactagggc tgtaaggaga ggcagcattg
    30421 tagaaaactt cggcagcctt ttccatttct tggacacttt ttccagctac ataagcgtag
    30481 acagcaactg ggacattccg cttttgaagt tcagtgatat gactcttata ggccttgtct
    30541 attccattga taaatgaagc atcattttct tttgttgttt gagcaccact gtgaacacga
    30601 acaatagcac ctgaaatatt ttgtgagagg gcatcgtagt tgatttcctc aggacgctgc
    30661 cagccagaga ggtcaataat cggtttgtct aagtgtttca aagcctgtgc ttcaatctgt
    30721 gctatattgg attttgtttt aaacgattgg ctgtcattaa gtgggcgatt gatgattaaa
    30781 atgaacatca taatcccaaa aaaactaaat aaaataagtg gatgaatttg ttttctcata
    30841 tcttataagt ctaccctaaa aatcaaaaaa aatcaaaaaa atgggttaag gaagagactt
    30901 tagagcattt tttcattcaa gagtgcggaa tgatttgaaa tatggtataa taaaagggaa
    30961 tttctacaga aaagagaaga ttatgtcaaa ttttgccatt attttagcag cgggtaaagg
    31021 gactcgcatg aaatctgatt tgccaaaagt tttgcacaag gttgcgggta tttctatgtt
    31081 ggaacatgtt ttccgtagtg tgggagctat ccaacctgaa aagacagtaa cagttgtagg
    31141 acacaaggca gaattggttg aggaggtctt ggctggacag acagaatttg tgactcaatc
    31201 tgaacagttg ggaactggtc atgcagttat gatgacagaa cctatcttag aaggtttgtc
    31261 aggacacacc ttggtcattg caggagatac tcctttaatc actggtgaaa gcttgaaaaa
    31321 cttgattgat ttccatatca atcataaaaa tgtggccact atcttgactg ctgaaacgga
    31381 taatcctttt ggctatggac gaattgttcg taatgacaat gctgaggttc ttcgtattgt
    31441 tgagcagaag gatgctacag attttgaaaa gcaaatcaag gaaatcaaca ctggaacata
    31501 cgtctttgac aacgagcgtt tgtttgaggc tttgaaaaat atcaatacca ataacgctca
    31561 aggcgaatac tatattacag acgtcattgg tattttccgt gaaactggtg aaaaagttgg
    31621 cgcttatact ttgaaagatt ttgatgaaag tcttggggta aatgaccgtg tggcgcttgc
    31681 gacagctgag tcagttatgc gtcgtcgcat caatcataaa cacatggtca acggtgttag
    31741 ctttgtcaat ccagaagcaa cttatatcga tattgatgtt gagattgctc cggaagttca
    31801 aatcgaagcc aatgttacct tgaaagggca aacgaaaatt ggtgctgaga ctgttttgac
    31861 aaacggtact tatgtagtgg acagcactat cggagcagga gcggtcatta ccaattctat
    31921 gattgaggaa agtagtgttg cagacggtgt gacagtcggt ccttatgctc acattcgtcc
    31981 aaattcaagt ctgggtgccc aagttcatat tggtaacttt gttgaggtga aaggatcttc
    32041 aatcggtgag aataccaagg ctggtcattt gacttatatc ggaaactgtg aagtgggaag
    32101 caacgttaat ttcggtgctg gaactattac agtcaactat gacggcaaaa acaaatacaa
    32161 gacagtcatt ggagacaatg tctttgttgg ttcaaattca accattattg caccagtaga
    32221 acttggtgac aattccctcg ttggtgctgg ttcaactatt actaaagacg tgccagcaga
    32281 tgctattgct attggtcgcg gtcgtcagat caataaagac gaatatgcaa cacgtcttcc
    32341 tcatcatcct aagaaccagt aggagcctat catggagttt gaagaaaaaa cgcttagccg
    32401 aaaagaaatc tatcaaggac caatatttaa actggtccaa gatcaggttg aattaccaga
    32461 aggcaaggga actgcccgac gggatttgat tttccacaat ggggctgtct gtgttttagc
    32521 agtaacggat gaacaaaaac ttatcttggt caagcagtac cgcaaagcta tcgaggctgt
    32581 ctcttacgaa attccagccg gaaaattgga agtaggagaa aacacagccc ctgtggcagc
    32641 tgcccttcgt gaattagagg aagaaacagc ctatacaggg aaattagaac tcttgtacga
    32701 tttttattca gctattggct tttgtaatga gaagttaaaa ctatatttag caagcgattt
    32761 gacaaaagtg gaaaatccgc gtccgcagga tgaggatgaa accttggaag tccttgaagt
    32821 gagcttagaa gaagcgaaag aattaatcca atcaggtcat atctgtgatg ccaagacaat
    32881 tatggctgtt cagtattggg agttgcagaa aaaatagagg aggtcagtat gggtaaatct
    32941 ttattaacgg atgaaatgat tgaaagagct aatagaggcg aaaaaatttc aggtcctcct
    33001 ttgctagatg ataatgagga aactaagatt ttaccaacct cttcttcccg ttttggttat
    33061 gccaatccta aggatcatgg ttttagccag gaaaccttga agattcaggt cgaaccatct
    33121 attcataaaa gccgtcgtat tgaaaatacc aagagaaatg tcttcaattc taagttgaat
    33181 aaaatcttat ttgcggccat ctttctcttg attttgcttg ttttagcaat gaaacttttg
    33241 taatagaaaa ggaattgaaa tgaaaatagg aattattgct gctatgccag aagaactggc
    33301 ttatctggtc cagcatttag ataataccca ggagcaagtt gttttgggga atacctatca
    33361 tacaggaacc attgcttctc atgaagtcgt tcttgtagaa agtggaattg gtaaggtcat
    33421 gtctgctatg agtgtggcga ttttggctga tcatttccag gtggatgccc ttattaatac
    33481 gggttcagct ggggcagtag cagaaggtat cgctgttggg gatgtcgtga ttgctgacaa
    33541 attagcctat catgacgtgg atgtcacagc ttttggctat gcttatggac aaatggcgca
    33601 acaaccgctt tatttcgaat cagacaaaac ctttgttgct caaatccaag agagtttatc
    33661 tcaattggac caaaactggc atcttggttt gattgctaca ggagatagtt ttgttgcagg
    33721 aaatgacaag atagaagcga ttaagtccca tttcccagaa gttttagccg tggagatgga
    33781 gggggcagct attgctcaag cagcgcatgc cctcaatctc ccagtcttag tcatcagagc
    33841 tatgagtgac aatgccaacc atgaagcaaa catctttttt gatgagttta ttatcgaagc
    33901 tggacgtcgc tctgcccaag tcttgttgac ctttttgaag gctttagatt aagcggaaat
    33961 ttgacagttt ttctagctta tgataagatt taagtaaaga aaagctagaa aacgtttcag
    34021 aggatattat gagtattgaa atgaccgtca gtgagattgc agaggtctta ggattatctc
    34081 gccaagcaat caataaccgt gtcaaagaat taccagaaga agacacagat aaaaatgaca
    34141 aaggggtaac agttgttacc agaagtggct tgattaagct agaagaaatc tataaaaaaa
    34201 cgatttttga agatgagcct gtcagtgaag atgtcaaaca acgtgaactg atggagattc
    34261 tagtggatga gaagaatgca gaaattcttc gtctttatga acaattaaaa gccaaggatc
    34321 gtcagttatc agaaaaagac gagcagatgc gtatcaaaga ccgtcagatt gctgagaaag
    34381 acaaacaatt agatcagcaa caacaattga cccttcaagc catgaaggat caagaaaatc
    34441 ttaagctaga gctggaccaa gcaaaagaag aagtccaatc cactaagaaa ggcttttttg
    34501 ctcgtttatt tggaggataa tcaaggagct gtttaggtta aatgctaacc tgatggcttc
    34561 ttttgtttgt gaatagtata gttgattaga acagagacag tagaggagag gtacagaatg
    34621 gaacgattag aagattacat tgcttttgac ttagaattta atcagcacga aggacaaaca
    34681 cacttgattc aagtatcggc agtgcgtttt agagatggtc gggaaatcga tgcctatgat
    34741 tcctatgttc ataccagcgt ccctctaaaa agctttatca atggtttgac cgggattaca
    34801 gctgagacct taaaagatgc tcctccagtt gagaaagtct tagaagaatt ccaagagttt
    34861 gtgggttctt tgctgatgat aggctacaat gctgctaaaa gtgatttacc tattttagca
    34921 gatcatggtt tagactatag ccaacagtac aaggtggatt tgtatgatga agcttttgaa
    34981 cgtcggagtt cagatttaca tggtattgcc aatctcaaat tgcaaactgt tgcctcattt
    35041 cttggatttc aaggtcagtc tcataatagt ttagaggatg ctcggatgac ggcgcgtgtt
    35101 tacgaatcct tcttagagtc ggatcaagct agggaattgt tagagacaga aagtagctta
    35161 tcaaacaatc cttttggtgt cttggacttg tctcaattat ttgactagga gtgcctatga
    35221 aacctgaaaa acctaaaaaa ttgggtctta aacagattta cctcaatctt gataaaatcc
    35281 tctttctatt tttcttgatt tttatgatag tggagtattt gtgggtacca ttcaattcat
    35341 ggttggctag cttcttatta gatcagacag gttatctttt tatttcctat aacaatgtct
    35401 tagccatcct tactcattct ccattgatta gtctagcttt tttagccttg attgtaatta
    35461 accttctggt tgcttatttt caaataggac tcctctttat tggtgctcgt catctcctct
    35521 ataatgaaaa gcgaaggcta attgagtata tccgcaaggt tttccatgaa agttttggat
    35581 ttatgaaaaa gctaacttgc gctaaagcct tgtttatctt tttctatata gctatgcttt
    35641 ttccttttat acggaaaatg ctgaagattt attatcttaa taaaatcgtc attccagagt
    35701 ttatccaagc ttatttagaa gataaatatt ggatgtggtg gctgagcatc ctacttctat
    35761 ctcttatctt tctctatgtc tctgtcagat tgatgtttgc ccttccgaag attgtatatg
    35821 atcagctgac tgttcgagaa gcagtgatgt ttagcttgga gaaaaccaag aagagagtta
    35881 ctttttatgc ttggaattta ttttacatct tgctcaaagc acatttatta ttttatcttc
    35941 ctttgattcc tttattattg gctcaaactt tggtagatga tatcactcag agagagtctc
    36001 tgattcttgg tattttgaat tttgtcgtga ttaaaaatct ctattatatg gctctgactt
    36061 attttttggt taaatttgtt tcatttttga caggaaagga gctggatatg cttcctagga
    36121 gagaaaaaga tcacatcgtg cgatggggtg tcatgacttg tgccagtctt ttctttgcct
    36181 tagaaggtta tatttatctg gaggctccca tggttcatct acctcaactt atttctcacc
    36241 gaggggtttc caatgcaaat gggattcaaa atacagtaga gtccttggaa actacagcac
    36301 aactcaaacc agacttggtg gagacggacg tgcaggaaac aaaagatggg cagtttgtca
    36361 tgatgcatga tgctaacttg aaaaatctag caggtatcaa taaaagtcct caagacttaa
    36421 acttggagga gcttaaaggg attgatattt ttgaaaatgg ctaccagact aaaatttcaa
    36481 gctttgaaga ttatctcagt cgagccaacg aacttggtca aaaattacta attgaaatta
    36541 aaaccagtaa aaaagatagt ccagacatga tgaaccgctt tttagcccgt tatgctgcaa
    36601 agctcaagat ttatggacat caaatccagt ctttagacta ccatgttgtc gaaaaagtaa
    36661 gacagtatga tgccgaactg ccagtttatt tcatcatgcc ctacaattct gtctttccta
    36721 aaacaagagc gacaggatat actatggagt actcaacctt ggatgaatat tttgtaagca
    36781 aactatggac aacggatcag aaactttatg tctggactgt caatgattca gaagctatca
    36841 gcaaatctct tcacttagga gtagatggag tgattacaga tgatttggaa aaagttcagc
    36901 gggaaataga agtagcccaa gaagatccag aatatacgga tttgctcttg aaaaaagcat
    36961 tggaattctt tgaattttag taataaaaaa gaggaacggt tgttcctccc atgataggta
    37021 agtattcttg tgaatgcttg cttttttgtg gttgcaatcc gtttacgaga attgtttgta
    37081 ttgttcttgt ttcaatttac tttatagtgc tttgaagttg gaatgctaca ctataacttc
    37141 taaaatatag taaaatgaac caaaaatagt acacaatgtg gtataatctt cttatggcat
    37201 attcaataga ttttcgtaaa aaagttctct cttattgtga gcgaacaggt agtataacag
    37261 aagcatcata cgttttccaa atctcacgta atactattta tggctggtta aagctaaaag
    37321 agaaaacagg agagctaaac caccaagtaa aaggaacaaa accaagaaaa gttgatagag
    37381 atagacttaa aaactatctt actgacaatc cagacgctta tttgactgaa atagcttctg
    37441 aatttggctg tcatccaact accatccact atgcgctcaa agctatgggc tacactcgaa
    37501 aaaagaacca cacctactat gaacaagacc cagaaaaagt agccttattt cttaagaatt
    37561 ttaatagttt aaagcaccta gcacctgttt agattgacga aacaggattc gatacttatt
    37621 tttatcgaga atatggtcgc tcattaaaag gtcagttaag aagaggcaaa gtatctggaa
    37681 gaagatatca gagaatttct ttggttgcag gtctaacaaa tgaatggtga gttaatcgct
    37741 ccaatgactt acgaagagac gatgacgagc gacttttttg aagtatggtt tcagaaattt
    37801 ctcttaccaa cattaaccac accatcggtt attatagtaa aatggaataa gaacaggaca
    37861 aatcgatcag gacagtaaaa tcgaattcta acaatgtttt agaagtctat atgtactatt
    37921 ctagtttcaa tataccataa ctgctcctag tcaacctgca agtcctgtcc cagctccaag
    37981 ctcagctccg actcaagatc ctagagtggt agcaagcgct tcggctagct caacgagcac
    38041 acaagctcaa gagcaagttg acaagtctga acttcgtgcc ttgagtcaag agttagacca
    38101 acgcttgaaa gctttggcaa cagtatctga tccaaaaatc gatgcaacca aggctgtcct
    38161 cctagatgct caaaaagctc cggaagatag tgccttgaca gagtaaggtc tgcatactgc
    38221 agtagagtct gtgaaggctg ccttggactc tctcaaagat gtgaaagcaa atgctagcga
    38281 cagcaagcct gcacaggaca agaaggatgc aaaacaagga acggaagata gtaaggattc
    38341 agataagatg actgaaacaa actcagttcc ggcaggagtg attgtggtca gtctacttgc
    38401 cctcctagac gtgattgcct tctggctgat tcgccgtaag aaagagtcag aaatccagca
    38461 attaagcacg gaattgatca aggttctagg acagctagat gcagaaaaag cggataaaaa
    38521 agtccttgcc aaagcccaaa accttctcca agaaaccctt gatttcgtga aagaagaaaa
    38581 tggctcagca gagacagaaa ctaaactagt agaggagctt aaagcaatcc ttgacaaact
    38641 caagtaaaac atgatttatg aaatgtcaga cctaaaagac attttcgttc acaaaagcag
    38701 tgacgagtaa tccttgtcgt tgctttttgg ctattttggg ggataagatc cgccttgact
    38761 agcaaaagaa aagattggtc caatagtgcc cgatacgatt ttgcgtagca gaaagcttac
    38821 aaataaaatt gaatatagta aaataggatt agaattatta agaaagttgg ttctttattg
    38881 gaaacgatag tatttttaat ctctgttttt ctagcaggtg ttttatcctt tttttctcct
    38941 tgtatttttc ctcttctgcc agtctatgct gggattttat tggatgatca agaaagtgca
    39001 aaaagctttt ctttgtttgg gagaaaggtt ctctggtcag gcttgattcg aacactttgc
    39061 tttatcgctg gtatctctct cattttcttt attctaggct ttggtgctgg ttactttggt
    39121 catattctct atgcaaattg gtttcgatat gtcatgggag ctattattat cattttgggt
    39181 cttcaccaga tggaaatttt tcatttgaag aaattagaag ttcaaaaaag ttttaccttt
    39241 aaaaaatcag attctaatcg ttattggtca gcttttttac ttggtattac ctttagcttt
    39301 ggttggacac cttgtattgg tccagtttta agttctgttt tagcacttgc ggcttctgga
    39361 ggcaatggtg cttggcaagg agcgatttat actctcattt acactctggg catggccctt
    39421 cctttcttgg tcttggcact agcttcaggt ctagtcatgc catattttag taaaatcaag
    39481 cgtcatatga tgctactaaa gaaaattggt ggtttcctca ttgttttaat gggaattttg
    39541 ttactattag gacaagtaaa tgttctagct ggaatttttg aataaaggag gacaaatgaa
    39601 aaaagtaatg tttgctggct taagtctctt gtcattagtt gtattgatgg cctgtggtga
    39661 ggaagaaact aaaaagactc aagcagcaca acagccaaaa caacaaacga ctgtacaaca
    39721 aattgctgtt ggaaaagatg ctccagactt cacattgcaa tccatggatg gcaaagaagt
    39781 taagttatct gattttaagg gtaaaaaggt ttacttgaag ttttgggctt catggtgtgg
    39841 tccatgcaag aaaagtatgc cagagttgat ggaactagcg gcgaaaccag atcgtgattt
    39901 cgaaattctt actgtcattg caccaggaat tcaaggtgaa aaaactgttg agcaattccc
    39961 acaatggttc caggaacaag gatataagga tatcccagtt ctttatgata ccaaagcaac
    40021 caccttccaa gcttatcaaa ttcgaagcat tcctacagaa tatttaattg atagccaagg
    40081 aaagattgga aagattcaat ttggtgctat cagtaatgcg gatgcagaag cagcatttaa
    40141 agaaatgaac tagaatcgat aaaatctgat tacaagatgt agtcagattt ttttagaaaa
    40201 acattttata aatattcaca tatctcctat atttatggta aaatagaatt atcagtttat
    40261 tttggagtca aagatgaata tatttagaac aaagaatgtt agtttagata aaacagagat
    40321 gcataggcat ttgaagttat gggatttgat tttgctgggt atcggagcca tggtagggac
    40381 aggcgtcttt acaatcacag gtactgcagc tgcaacactt gctggcccag ccctagtgat
    40441 ttcaatcgtt atttctgcct tgtgtgtggg attatcagcc ctcttttttg cagaatttgc
    40501 ctcgcgagta cccgctacag gaggtgccta tagttacctc tatgctatct taggagaatt
    40561 ccctgcctgg ttggctggtt ggttaaccat gatggagttc atgacagcca tatcaggcgt
    40621 agcttcgggt tgggcagctt attttaaagg cttgctctct caatacggga tagcctttcc
    40681 tcaggcttta aatggtacct ttaatcctca agcagggaca tttgttgatt tattgcctat
    40741 tcttgtcttg gtcttggtga cttcgcttgt tttactaaat gccaaggcag ccttacgctt
    40801 taattcgatt ctagttattt tgaaattttc cgctttagct ctctttgttt tagtaggaat
    40861 ttggaatatt aagtttgata attggagcaa ttttgctcct tacggttttg gacaaatcta
    40921 tggagctagc actggtatta tggctggtgc gtccttgatg ttcttcggtt ttttgggatt
    40981 tgaatccatc tctatggctg tagatgaagt caagactcct caaaaaaata ttcctagagg
    41041 gattgtctta tcgctttcta tcgtaaccat tctctatgcc ttggtgaccc ttgttttgac
    41101 tggtgtggtt cactatagtc atttaaatgt cgatgatgcc gttgcctttg cccttcgtag
    41161 tgttgggatt agttgggcag ccaactatgt gtcattagtg gctatcttga ccttgattac
    41221 agtttgtatt tcgatgacct atgccctatc gcgtatgatt tacagtttag cacgtgacgg
    41281 cttagtgcct gctgccttta aggaactgac gaagactagc aagataccaa agaatgctac
    41341 tatcttgaca ggtctagctt cagcagtagc agcaggaatg ttcccactag ccagtattgc
    41401 agccttttta aatatttgta ccttagccta cttgatcatg ctggcttatg gtctgattcg
    41461 cttacggaaa gaaaaaggaa tgcccaaagc tggggaattt aaaacaccat tggtacctct
    41521 gttaccaatt ttatcgatca tcatttgttt atccttcatg ttacagtata atatgaacac
    41581 ctggattgct ttcctagttg cgttgttagt aggaagtatt atttacttca cttatggcta
    41641 taagcattct accatagaag aataaagtga aagtctattg agttgttgac actcagtaga
    41701 tttttatatg tgaaagaaat ttcaaatttt ttctaaaaat atcttgacag attaaatttt
    41761 caggagtaga atatttacta gttaattaaa tggttaagga gttgttcatg aagaaacaaa
    41821 atttattttt agtcctgtta agtgtctttc ttttgtgttt gggggcttgt ggtcaaaagg
    41881 aaagtcagac aggaaagggg atgaaaattg tgaccagttt ttatcctatc tacgctatgg
    41941 ttaaggaagt atctggtgac ttgaatgatg ttcggatgat tcagtcaagt agtgggattc
    42001 actcctttga accttcggca aatgatatcg cagccatcta tgatgcagat gtctttgttt
    42061 accattctca tacactcgaa tcttgggcag gaagtctgga tccaaatcta aaaaaatcca
    42121 aagtgaaggt cttagaggct tctgagggaa tgaccttgga acgtgtccct ggactagagg
    42181 atgtggaagc aggggatgga gttgatgaaa aaacgctcta tgaccctcac acatggctag
    42241 atcctgaaaa agctggagaa gaagcccaaa ttatcgctga taaactttca gaggtggata
    42301 gtgagcataa agagacttat caaaaaaatg cgcaagcctt tatcaaaaaa gctcaggaat
    42361 tgactaagaa attccaacca aaatttgaaa aagcgactca gaaaacattt gtaacacaac
    42421 atacagcctt ttcttatcta gcgaagagat ttgggcttaa tcaacttggt attgcaggta
    42481 tctctcctga acaagaacca agtccacgac aactaacaga aattcaggaa tttgttaaga
    42541 cctataaggt taaaacgatt tttacagaaa gtaacgcttc ttcaaaagta gctgaaactc
    42601 ttgtcaaatc aacaggtgtg ggtcttaaaa ctctgaatcc tttagagtca gacccacaaa
    42661 atgacaagac ctatttagaa aatcttgaag aaaatatgag tattctagca gaagaattaa
    42721 agtgaggaaa gaatgaaaat caataaaaaa tatctagcag gttcagtggc agtccttgcc
    42781 ctaagtgttt gttcctatga acttggtcgt caccaagctg gtcaggttaa gaaagagtct
    42841 aatcgagttt cttatataga tggtgatcag gctggtcaaa aggcagaaaa tttgacacca
    42901 gatgaagtca gtaagagaga ggggatcaac gccgaacaaa ttgttatcaa gattacggat
    42961 caaggttatg tgacctctca tggagaccat tatcattact ataatggcaa ggttccttat
    43021 gatgccatca tcagtgaaga acttctcatg aaagatccga attatcagtt gaaggattca
    43081 gacattgtca atgaaatcaa gggtggctat gtgattaagg tagacggaaa atactatgtt
    43141 taccttaaag atgcagccca tgcggacaat attcggacaa aagaagagat taaacgtcag
    43201 aagcaggaac gcagtcataa tcacaactca agagcagata atgctgttgc tgcagccaga
    43261 gcccaaggac gttatacaac ggatgatggg tatatcttca atgcatctga tatcattgag
    43321 gacacgggtg atgcttatat tgtccctcac ggcaatcact tccactatat tcctaagagt
    43381 gatttgtctg ccagtgaatt agctgctgcc caagcctatt ggaatgggaa gcagggatct
    43441 cgtccttctt caagttctag tcataatgca aatccagctc aaccaagatt gtcagagaac
    43501 cacaatctga ctgtcactcc aacttatcat caaaatcaag gggaaaacat ttcaagcctt
    43561 ttacgtgaat tgtatgctaa acccttatca gaacgccatg tagaatctga tggcctagta
    43621 tttgatccgg cacaaataac atatcgtaca gtcaatggtg ttgctgtacc gcacggagac
    43681 cattatcact ttattcctta ttcacaactg tcacctttgg aagaaaaatt ggctcgtatg
    43741 attgctgtta agggacaaaa tggtgcagtc ttgccaggaa tgcattattt gaaaccagca
    43801 ccaaaaccgc aagttcaacc atcaactgag aaaaaacaaa ctgattttgc agtagaacag
    43861 gttgttagaa aagttggaga aggctatgtt gtggaaattg ctggtgtcag tcactatgtt
    43921 tttgctaaag acttggctaa ggataagatt gatgctattg aaaatctctt gtctaagaaa
    43981 actcaagaga cacatgctct agttgctaaa aaagaaaatg tcgctcctcg tgaccaagaa
    44041 ttttatgata aagcttacaa tctgttaacc caggctcata aagtcttgtc tgaaaataaa
    44101 ggtcgtactt ctgatttcca agccttagac aaattagcag aacgcttgaa taatgaatct
    44161 tctaataaag taaaattggt agatgattta ttggcattcc tagcaccaat tacccaccca
    44221 gaacgccttg g
//
LOCUS       DAVKQB010000012        42092 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKQB010000012 DAVKQB010000000
VERSION     DAVKQB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.012"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..175)
                     /locus_tag="VW528_000643"
                     /pseudo
     CDS             complement(1..175)
                     /locus_tag="VW528_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(270..455)
                     /locus_tag="VW528_000644"
                     /pseudo
     CDS             complement(270..455)
                     /locus_tag="VW528_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            483..710
                     /locus_tag="VW528_000645"
     CDS             483..710
                     /locus_tag="VW528_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9911502.1"
                     /translation="MRERLLTQPLLHADETSYRVLESHSHLTYYWTFLSGKVENQAIT
                     LYHHDQSRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            786..1338
                     /locus_tag="VW528_000646"
                     /pseudo
     CDS             786..1338
                     /locus_tag="VW528_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1922..2398
                     /locus_tag="VW528_000647"
     CDS             1922..2398
                     /locus_tag="VW528_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9911503.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            2436..2741
                     /locus_tag="VW528_000648"
     CDS             2436..2741
                     /locus_tag="VW528_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9911504.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            2789..4264
                     /locus_tag="VW528_000649"
     CDS             2789..4264
                     /locus_tag="VW528_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9911505.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            4290..4367
                     /locus_tag="VW528_000650"
                     /pseudo
     CDS             4290..4367
                     /locus_tag="VW528_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            4569..11270
                     /locus_tag="VW528_000651"
     CDS             4569..11270
                     /locus_tag="VW528_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9911506.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIENRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLYKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVKVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKDQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     VPKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAIKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSFPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKIEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKVGK
                     ERIFTAINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPSTGSAASQAAVAAGLTLLGLSAGLVVTKGKKED"
     gene            complement(11518..12374)
                     /locus_tag="VW528_000652"
                     /pseudo
     CDS             complement(11518..12374)
                     /locus_tag="VW528_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12584..12943)
                     /locus_tag="VW528_000653"
     CDS             complement(12584..12943)
                     /locus_tag="VW528_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9911507.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            12966..13544
                     /locus_tag="VW528_000654"
     CDS             12966..13544
                     /locus_tag="VW528_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846832.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEU9911508.1"
                     /translation="METRNRGIYRKVRLLRLWVIFVIMKVMKSYNTLNDYYRKLFGEK
                     TFKVPIDAGFDCPNRDGTVAHGGCTFCTVSGSGDAIVAPDAPIREQFYKEIDFMHRKW
                     PDVQKYLVYFQNFTNTHEKVEVVRERYEQAINEPGVVGINIGTRPDCLPDETIEYLAE
                     LSECMHVTVELGLQTTYEATSDLINRAHSYEL"
     gene            13545..13991
                     /locus_tag="VW528_000655"
     CDS             13545..13991
                     /locus_tag="VW528_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU9911509.1"
                     /translation="METVKRLRKYPKIEIVSHLINGLPGETHEMMVENVRRCVTNNDI
                     QGIKLHLLHLMTNTRMQRDYHEGRLQLMSQDEYVRVICDQLEIIPKHIVIHRITGDAP
                     RDMLLGLMWSLNKWEALNSIEMEMRRRGSVQGCKAVKQEFENEKTT"
     gene            13975..14532
                     /locus_tag="VW528_000656"
     CDS             13975..14532
                     /locus_tag="VW528_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9911510.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            14525..14779
                     /locus_tag="VW528_000657"
     CDS             14525..14779
                     /locus_tag="VW528_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911511.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            14791..16845
                     /locus_tag="VW528_000658"
     CDS             14791..16845
                     /locus_tag="VW528_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9911512.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            17024..17884
                     /locus_tag="VW528_000659"
     CDS             17024..17884
                     /locus_tag="VW528_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9911513.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            18095..18805
                     /gene="ccdA2"
                     /locus_tag="VW528_000660"
     CDS             18095..18805
                     /gene="ccdA2"
                     /locus_tag="VW528_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9911514.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGFLVLFGNASILSQLFE"
     gene            18825..19391
                     /locus_tag="VW528_000661"
     CDS             18825..19391
                     /locus_tag="VW528_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9911515.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            19402..20514
                     /gene="msrB"
                     /locus_tag="VW528_000662"
     CDS             19402..20514
                     /gene="msrB"
                     /locus_tag="VW528_000662"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9911516.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            20872..21609
                     /locus_tag="VW528_000663"
     CDS             20872..21609
                     /locus_tag="VW528_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9911517.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            21606..23297
                     /locus_tag="VW528_000664"
     CDS             21606..23297
                     /locus_tag="VW528_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9911518.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            23413..23976
                     /locus_tag="VW528_000665"
     CDS             23413..23976
                     /locus_tag="VW528_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9911519.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            23998..29940
                     /locus_tag="VW528_000666"
     CDS             23998..29940
                     /locus_tag="VW528_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEU9911520.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAEAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPREDEKAPVEPEKQPEAPEEEKAVEETPKQEDTQPEVVETKDEAANQP
                     VEEPKVETPAVEKQTEPTEEPKVEQVGEPVEPREDEKAPVSPEKQPEAPEEEKTAEET
                     PKQEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYSTASYNALGPVLETAKGVYASE
                     PVKQPEVNSETKAEKVAANTDAKQSEVNSETASLKTAISGLNTDKVELENQLKIAQGK
                     TETDFSMESWTVLSTAKNKAQEVKDNGTATQEQINEAEKSLKTALADLSVDKTALGSA
                     IDTATKKNKENYTNQTWAELETVLTAAKSVNTNESKQSEVNEAVEKLTATIEKLVELS
                     EKPRLTLSIEKRDIDRKVTVTYTLENPANTQIKSITATLKKGEEVVKDFVLTEENLKT
                     NHLTALFEKLDYYKEYTLSTDMVYNRGNDDETESISEELIQLNLKKLELKDIQTVSLM
                     KFENGQESQVTHLSDKPTDLSKLYLKVTSSTSKDAVLAVSSIEEEIVENKKIFKIHAD
                     TPELVVRKKDGSLSKGFDYYMERVIPHDGDIYYDFKDLISAMTSNPTGTFILGRDISS
                     RNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIFDLEYPLFDTIKSGVVKDIDFKHVNM
                     VFPDSNQGDNVATIARVIKDKTKIENVNVEGYLEGRDHVAGLVNNLEGNSEIENISFT
                     GKIKSKGGNSITAGIAGRNILSRVKRAYVNANIEVLGSTNSSMLVAVNGTTLNASGGW
                     GAWGRLTESVAKGTLEIKRSGQAGGVTATVWPYGAIDKVVSYAKVTKGKELFGSDGDL
                     NNNWFMQKINNIFGVQGISSGDSGNDSKFKRISEEEAKQKVASYNITAPNLMSDSSLL
                     VDRLNESWKNTDQFESIQDYQSQNQLIYQNLTKFTPYYNKEFIVHEGNALTPEQEILK
                     TKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGSQKRYKVTSTGKFSITNLPEYQVEDL
                     NVVYTSEHIVHPLDSSLINNLVEELKKVELYTESTYQVLGIDKDNANKLNRTKRLFLD
                     ESLDAVKTQLPTFVKTMFENEWLHINGESSGAVAALRQKIMDNKTAILLALTYINRYY
                     DVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRLGSSGENRLKGSENAETFKQLFASET
                     KQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDYIQFEERPSLVEEIKDAKYRVYDNLT
                     APYYQGYILPLLTLKNTHLAILSNYSTMTFVSREKRPNWKNEDFDKWVKYVATAHRNH
                     VDTWYKILPDNIKGKMVKENVTAVWEGLSIPGSEWVDQNAVDRKGRDYAPAREFFNLV
                     GGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSEYGISVFTHELTHVNDTWIYLGGYGR
                     RENMGPEAYAQGLFQSPVPGQPGWGALGLNMAFERKNDGDLIYNASPTQFENRKELDS
                     YMKNYNDTLMMVDYLEGDAVISKGKEAITKWFKKVEPKVVSQTAQYDTVRQLTAEEKE
                     KLSVSSVDDLVDQGLMSDRAVGNNTYNPADFETSYIAIDYMTGIYGGGKNSVGSPGAL
                     MFKHNTFRMWGYYGFEEGVLGYASNKFKQASRDEGHAGLSDNFIISKISKGEFLTMEA
                     FKKGYFKKVVEELKTKGIRPVTINQKTYSTFEELQEGFKQAVERDLKKNQLDERETRN
                     FKFQVFRQLLQQTDSFKTSIFR"
     gene            30001..31722
                     /gene="pabB"
                     /locus_tag="VW528_000667"
     CDS             30001..31722
                     /gene="pabB"
                     /locus_tag="VW528_000667"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9911521.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKNLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            31723..32340
                     /locus_tag="VW528_000668"
     CDS             31723..32340
                     /locus_tag="VW528_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9911522.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SFMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            32390..33388
                     /locus_tag="VW528_000669"
     CDS             32390..33388
                     /locus_tag="VW528_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9911523.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEREPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTV
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            33515..34474
                     /locus_tag="VW528_000670"
     CDS             33515..34474
                     /locus_tag="VW528_000670"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9911524.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            34571..35410
                     /locus_tag="VW528_000671"
     CDS             34571..35410
                     /locus_tag="VW528_000671"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9911525.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRIDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(35458..35628)
                     /locus_tag="VW528_000672"
     CDS             complement(35458..35628)
                     /locus_tag="VW528_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9911526.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            35749..36633
                     /gene="miaA"
                     /locus_tag="VW528_000673"
     CDS             35749..36633
                     /gene="miaA"
                     /locus_tag="VW528_000673"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9911527.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            36626..37864
                     /gene="hflX"
                     /locus_tag="VW528_000674"
     CDS             36626..37864
                     /gene="hflX"
                     /locus_tag="VW528_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9911528.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            37857..38480
                     /locus_tag="VW528_000675"
     CDS             37857..38480
                     /locus_tag="VW528_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9911529.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            38495..39424
                     /locus_tag="VW528_000676"
     CDS             38495..39424
                     /locus_tag="VW528_000676"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9911530.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            39445..40200
                     /locus_tag="VW528_000677"
     CDS             39445..40200
                     /locus_tag="VW528_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9911531.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(40254..41162)
                     /locus_tag="VW528_000678"
     CDS             complement(40254..41162)
                     /locus_tag="VW528_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9911532.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            41226..41495
                     /locus_tag="VW528_000679"
     CDS             41226..41495
                     /locus_tag="VW528_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9911533.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            41495..41875
                     /locus_tag="VW528_000680"
     CDS             41495..41875
                     /locus_tag="VW528_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9911534.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
BASE COUNT        12927 a         7753 c         9363 g        12049 t
ORIGIN      
        1 ttgacaatct tctctttatc tttgaggaag gttttagagg atgaacctga ttcagattgt
       61 cctcaatgag tccgaaaaat ttgtcaggct ccttattctg aaagtgaaaa agcaagagtt
      121 gatagagatt atagtggtgt ttcaagtctt ctgaatagct caaaagttta tctatagtag
      181 attgaaacta gaatagtaca cctctgcttc taaaacattg ttagaaatcg atttgactgt
      241 cctgaatgat ttgtcctgtt attatttcat tttactataa atccacgttt acgaatctct
      301 ttccacactt gttcaatggg gttcatctct ggtgtgtatg gaggaataaa tgcaaaacca
      361 atattagtcg gaatctttaa ggtacttgat ttatgccata tagcattgtc cataacgagt
      421 aaaagataat catctggata agcttgtgaa agctcctatt cctaaagccc ctttataacc
      481 tcttgcgaga gagactattg actcagccct tacttcatgc ggatgaaact tcttatcggg
      541 ttctagagag tcatagccat ctgacctact attggacctt tttgtctggg aaagttgaga
      601 atcaagcaat cacgctgtac catcatgatc agagtcggag tggttcggta gtacaagaat
      661 tcctaggaga ttattctggc tatgttcatt gtgatatgtt gcggcagtaa cttaggactt
      721 tagtcctcta gttctgccta tgcgatagca gtccaaggtt taggagcaag gcgacgctaa
      781 gcttggtaaa ctgcgaactg ctagaagctt atcgtcaact ggaagaagct gaacttgttg
      841 gatgttgggc gcatgtgaga aggaaatttt ttgaagcgac ccccaagcaa gcagataaat
      901 catccttagg agctaaaggt ttagcttatt gtgatcagtt attttccttg gaaagagact
      961 gggaggcttt gccagctgat gaacgactac agaaacgtca agaacatctc cagcccctaa
     1021 tggaagactt ctttgcttgg tgccgccgtc agtcagtttt agcaggttca aaactaggaa
     1081 gggcaattga atacagcctc aagtatgaag aaacctttaa gactattttg aaagacggac
     1141 atctggtcct ttccaataat ctagctgaac gcgccattaa atcattggtt atgggacgga
     1201 gtaaaagagt ccagtggact cttttagcct gagctcagtt taaaaaagcg agggtggtta
     1261 ttttctcaaa gttttgaagg agctaaagca agagctatta ttatgagctt gttggaaaca
     1321 gctaaacgtc atcaattata gtgcgttgaa tctataacag tacgcatcga ctgctaaaac
     1381 atttctataa atcaattttc ctttcctaat cgatttgttc atatcttatt tcaatccatt
     1441 ataaatagcg agaaatatct atcctatctt ctagaatgtc ttccaaacga ggaaactctc
     1501 gtaaacaaag aggttttaga ggcctattta ccatggacta aagttgtaca agaaaagtgc
     1561 aaataagaaa tctccagatt aggaactatc cgtgagttca ctaatctgga gatttttcaa
     1621 tagacttcgt tattgggcgg ttacgatatg atcactgact tcgtcagtct tatctacaac
     1681 ctcaaaactg tgttttgagc aacctgcggc tagcttccta gtttgctctt tgattttcat
     1741 tgagtattag aacagaaaaa atacttggag tatttgtttg tgtgtttatt tttatataac
     1801 aaactataaa caaaacaaaa atataaaaaa agagacaaaa aaagaacaga aagcaattga
     1861 caacggtttc atatagtgtt atacttatgg cataaagtta atggaaagaa gggaaaaact
     1921 tatggtatta gattatttct ttgacaaaaa ccttgtgttt tgcttagaag cggataatca
     1981 agaacaactc tttgatcagg ttgcaacttt attggaagaa cgagaaattg tcactccaac
     2041 ttatcgtgaa gccttgatca cgcgtgaaaa gtcatttcca actggtttag atatggaatt
     2101 tctaggaaag gacttgccaa atgtagcgat tcctcataca gatattgttc ataatctagc
     2161 tgagaaagtg gtggttgttc gattagaaaa accagtaact tttcacaata tgatagctcc
     2221 tgataaggaa gtagaagtat ccctactctt ctttattatt aacaattcaa gttcaagtca
     2281 aacaaatatt ctggctcagt tgatggactt tttcacagga aatggacatc ttgaagacct
     2341 atcaaaaatt tccgaaccag aaaaacttta tgcttacatt gctgaagcaa ccgcttaatc
     2401 ttgtctatta aaaaataaaa tcggaggaaa tccaaatgat taaaattctt gctgcctgcg
     2461 gtgcaggtgt taactcaagt caccaaatta aaagtgctct agaagaagaa ctttcaaacc
     2521 gtggttatga tgttcactgt gatgcagtca tggtgaaaga tgtaaacgaa gaccttatga
     2581 aaggttacga tatctttaca ccaatcgctg caacagacct tggttttgaa ccaggtatcc
     2641 cagttatcga agctgggcca atcttattcc gtatcccagc aatgagcgct ccagtatttg
     2701 acaatattga agcagctatt aaagaacacg gattaagcta attctaattt gttaacattc
     2761 atataacata aaaaagggag gaactgttat ggatgcaatc tttgacctaa tcggaaaggt
     2821 tttcaatccc attttagaaa tgggtggacc tgtcatcatg ttaatcattt tgacagtatt
     2881 ggctttactt tttggagtga aattctccaa agcgcttgaa ggtggtatca aacttgccat
     2941 cgctcttaca ggtatcggtg ctatcatcgg tatgctaaat ggtgctttct cagcatcact
     3001 tgcaaaattc gttgaaaaca ctggtatcca attgagtatt accgacgttg gttgggcacc
     3061 actcgcaaca atcacttggg gttctgcttg gacactatac ttcttgctca tcatgttgat
     3121 tgtcaacata gtgatgctag ctatgaagaa aacagataca cttgacgttg atatctttga
     3181 tatctggcac ttgtctatca caggtctctt gattaaatgg tatgctgata acaatggtgt
     3241 gagtcaaggg gtttcactct ttattgctac agcagctatc gtccttgtcg gtgtgttgaa
     3301 aattatcaac tctgacttga tgaaacctac atttgatgac cttcttaacg ccccaagttc
     3361 atcaccaatg acatcaactc acatgaacta catgatgaac ccagttatca tggttttgga
     3421 taagattttt gaaaaattct tcccaggcct tgataaatat gactttgatg ctgctaaatt
     3481 gaacaagaaa atcggtttct ggggatctaa attcttcatc ggtttcatcc ttggtatcgt
     3541 tatcggtatt atgggaactc cacatccaat tgcaggtgtt gcagatgcag ataaatggcg
     3601 tcttgttatc aaaggatggt tgtctcttgg tttgactgcc ggtgtatctt tggaactctt
     3661 ctcacttatc ggttcatggt tcatcgcagc cgtagaacca ctatcacaag gtattacaaa
     3721 cgttgctact aaacgtcttc aaggacgtaa attcaatatc ggtcttgact ggccattcat
     3781 cgctggtcgt gctgaaatct gggcttgtgc caacgtactt gcaccaatca tgttgattga
     3841 agcagtgctt ctttcaaaag ttggaaatgg tatcttgcca cttgcaggta tcatcgctat
     3901 gggtgttact ccagctctct tggttgtaac tcgtggtaaa ttgctccgta tgattatctt
     3961 cggaacactc ttgttgccac tcttccttct ttcaggtaca cttattgcac catttgcaac
     4021 agaacttgct aaaggtgtag gtgccttccc agaaggtgtg agccaaactc aattgattac
     4081 tcactctact cttgaaggac caatcgaaaa acttcttggt tggacaattg gtaacactac
     4141 aactggtgat atcaaagcaa tccttggtgc agtagtcttc cttgtattct atatcggtat
     4201 ctttgcttgg tacagaaaac aaatgatcaa acgtaacgaa gagtacgcag caaaagcaaa
     4261 ataatgcgct cctataaaat ataaactcaa tgaaaatcaa agagcaaact aggaaactag
     4321 ccacaggttg ctcaaaacac agttttgagg ttgtagataa gactgacgaa gtcagctcaa
     4381 aacatggttt tgaggttgta gatgaaactg acgaagtcag taatcatatc tacggcaagg
     4441 cgacactgac gaagtttgaa ttggattttc gcagagtgta aattgttgaa actaggttgt
     4501 cataccatgt ataccacttg ggctttggca gtagctaact gcactaaata taatataagg
     4561 agggttttat ggggaaaggc cattggaatc ggaaaagagt ttatagcatt cgtaagtttg
     4621 ctgtgggagc ttgctcagta atgattggga cttgtgcagt tctattagga ggaaatatag
     4681 ctggagaatc tgtagtttat gcggatgaaa cacttattac tcatactgct gagaaaccta
     4741 aagaggaaaa aatgatagta gaagaaaagg ctgataaagc tttggaaact aaaaatgtag
     4801 ttgaaaggac agaacaaagt gaacctagtt caactgaggc tattgcatct gagaagaaag
     4861 aagatgaagc cgtaactcca aaagaggaaa aagtgtctgc taaaccggaa gaaaaagctc
     4921 caaggataga atcacaagct tcaagtcaag aaaaaccgct caaggaagat gctaaagctg
     4981 taacaaatga agaagtgaat caaatgattg aaaacaggaa agtggatttt aatcaaaatt
     5041 ggtactttaa actcaatgca aattctaagg aagccattaa acctgatgca gacgtatcta
     5101 cgtggaaaaa attagattta ccgtatgact ggagtatctt taacgatttc gatcatgaat
     5161 ctcctgcaca aaatgaaggt ggacagctca acggtgggga agcttggtat cgcaagactt
     5221 tcaaactaga tgaaaaagac ctcaagaaaa atgttcgcct tacttttgat ggcgtctaca
     5281 tggattctca agtttatgtc aatggtcagt tagtggggca ttatccaaat ggttataacc
     5341 agttctcata cgatatcacc aaataccttt acaaagatgg tcgtgagaat gtgattgctg
     5401 tccatgcagt caacaaacag ccaagtagcc gttggtattc aggaagtggt atctatcgtg
     5461 atgtgacttt acaagtgaca gataaggtgc atgttgagaa aaatgggaca actattttaa
     5521 caccaaaact tgaagaacaa caacatggca aggttgaaac tcatgtgacc agcaaaatcg
     5581 tcaatacgga cgacaaagac catgaacttg tagccgaata tcaaatcgtt gaacgaggtg
     5641 gtcatgctgt aacaggctta gttcgtacag cgagtcgtac cttaaaagca catgaatcaa
     5701 caagcctaga tgcgatttta gaagttgaaa gaccaaaact ctggaccgtt ttaaatgaca
     5761 aacctgcctt gtacgaattg attacgcgtg tttaccgtga cggtcaattg gttgatgcta
     5821 agaaggattt gtttggttac cgttactatc actggactcc aaatgaaggt ttctctttga
     5881 atggtgaacg tattaaattc catggagtat ccttgcacca cgaccatggg gcgcttggag
     5941 cagaagaaaa ctataaagca gaatatcgcc gtctcaaaca aatgaaggag atgggagtta
     6001 actccatccg tacaacccac aaccctgcta gtgagcaaac cttgcaaatc gcagcagaac
     6061 taggtttact cgttcaggaa gaggcctttg atacttggta tggtggcaag aaaccttatg
     6121 actatggacg tttctttgaa aaagatgcca ctcacccaga agctcgaaaa ggtgaaaaat
     6181 ggtctgattt tgacctacgt accatggtcg aaagaggcaa aaacaaccct gctatcttca
     6241 tgtggtcaat tggtaatgaa ataggtgaag ctaatggtga tgcccactct ttagcaactg
     6301 ttaaacgttt ggtcaaggtt atcaaggatg ttgataagac tcgctatgtt accatgggag
     6361 cagataaatt ccgtttcggt aatggtagcg gagggcatga gaaaattgct gatgaactcg
     6421 atgctgttgg atttaactat tctgaagata attacaaagc ccttagagct aagcatccaa
     6481 aatggttgat ttacggttca gaaacatcat cagcaacccg tacacgagga agttactatc
     6541 gccctgaacg tgaattgaaa catagcaatg gacctgagcg taattatgaa cagtcagatt
     6601 atggaaatga tcgtgtgggt tgggggaaaa cagcaaccgc ttcatggact tttgaccgtg
     6661 acaacgctgg ctatgctgga cagtttatct ggacaggtac ggactatatt ggtgaaccta
     6721 caccatggca caaccaaaat caaactcccg ttaagagctc ttactttggt atcgtagata
     6781 cagccggcat tccaaaacat gacttctatc tctaccaaag ccaatgggtt tctgttaaga
     6841 agaaaccgat ggtacacctt cttcctcact ggaactggga aaacaaagaa ttagcatcca
     6901 aagtagctga ctcagaaggt aagattccag ttcgtgctta ttcgaatgct tctagtgtag
     6961 aattgttctt gaatggaaaa tctcttggtc ttaagacttt caataaaaaa caaaccagcg
     7021 atgggcggac ttaccaagaa ggtgcaaatg ctaatgaact ttatcttgaa tggaaagttg
     7081 cctatcaacc aggtaccttg gaagcaattg ctcgtgatga atctggcaag gaaattgctc
     7141 gagataagat tacgactgct ggtaagccag cggcagttcg tcttattaag gaagatcatg
     7201 cgattgcagc agatggaaaa gacttgactt acatctacta tgaaattgtt gacagccagg
     7261 ggaatgtggt tccaactgct aataatctgg ttcgcttcca attgcatggt caaggtcaac
     7321 tggtcggtgt agataacgga gaacaagcca gccgtgaacg ctataaggcg caagcagatg
     7381 gttcttggat tcgtaaagca tttaatggta aaggtgttgc cattgtcaaa tcaactgaac
     7441 aagcagggaa attcaccctt actgcccact ctgatctctt gaaatcgaac caagtcactg
     7501 tctttactgg taagaaagaa ggacaagaga agactgtttt ggggacagaa gtgccaaaag
     7561 tacagaccat tattggagag gcacctgaaa tgcctaccac tgttccgttt gtatacagtg
     7621 atggtagccg tgcagaacgt cctgtaacct ggtctcaagt cgatgttagt caagcaggag
     7681 ttgtgactgt taaaggtaca gcaaatggac gtgaggtaga ggctcatgtc aaggttctag
     7741 caattgcgaa agaattacca acagttaaac gtattgctcc aaatactgac ttgaattctg
     7801 tagacaaatc tgtttcttat gttttgactg atggaagtgt acaagagtac gaagtagata
     7861 gctgggagat tacggaagta gataaagcca aactttcagt agctggatca cgtattcaaa
     7921 tgactggtca gttagctgga gaaactattc atgcaaccct tgtggtagaa gaaggaaatg
     7981 ctgcagcacc tgtagtgcca actgttactg ttggaggtga agcagtaaca ggtcttacta
     8041 gtcgacaacc aatgcaatat cgtactctat cttatggtgc ccaattgcca gaagtcacag
     8101 caagtgctga aaatgctgat gtgacagttc ttcaagcaag cgcagcaaac ggcatgcgtg
     8161 cgagcatctt tattcagcct aaagatggtg gccctcttca aacctatgca attcaattcc
     8221 ttgaagaagc gccaaaaatt gctcacttga gcttgcaagt ggaaaaagct gacagtctca
     8281 aagaagacca aactgtcaaa ttgtcggttc gagctcacta tcaagatgga acgcaagctg
     8341 tattaccagc tgataaagta accttctcta caagtggtga aggggaagtc gcaattcgta
     8401 aaggaatgct tgagttgcat aagccaggag cagtcactct gaacgctgaa tatgagggag
     8461 ctaaagacca agttgaactc actatccaag ccaatactga gaagaagatt gcgcaatcca
     8521 tccgtcctgt aaatgtagtg acagatttgc atcaggaacc aagtcttcca gcaacagtaa
     8581 cagttgagta tgacaaaggt ttccctaaaa ctcataaagt cacttggcaa gctattccga
     8641 aagaaaaact agactcctat caaacatttg aagtactagg taaagttgaa ggaattgacc
     8701 ttgaagcgcg tgcaaaagtc tctgtagaag gtatcgtttc agttgaagaa gtcagtgtga
     8761 caactccaat cgcagaagca ccacaattac cagaaagtgt tcggacatat gattcaaatg
     8821 gtcacgtttc atcagctaag gttgcatggg atgcgattcg tccagagcaa tacgctaagg
     8881 aaggtgtctt tacagttaat ggtcgcttag aaggtacgca attaacaact aaacttcatg
     8941 ttcgcgtatc tgctcaaact gagcaaggtg caaacatttc tgaccaatgg accggttcag
     9001 aattgccact tgcctttgct tcagactcaa atccaagcga cccagtttca aatgttaatg
     9061 acaagctcat ttcctacaat aaccaaccag ccaatcgttg gacaaactgg aatcgtacta
     9121 atccagaagc ttcagtcggt gttctgtttg gagattcagg tatcttgagc aaacgctccg
     9181 ttgataatct aagtgtcgga ttccatgaag accatggagt tggtgtaccg aagtcttatg
     9241 tgattgagta ttatgttggt aagactgtcc caacagctcc taaaaaccct agttttgttg
     9301 gtaatgagga ccatgtcttt aatgattctg ccaactggaa accagttact aatctaaaag
     9361 cccctgctca actcaaggct ggagaaatga accactttag ctttgataaa gttgaaacct
     9421 atgctgttcg tattcgcatg gttaaagcag ataacaagcg tggaacgtct atcacagagg
     9481 tacaaatctt tgcgaaacaa gttgcggcag ccaagcaagg acaaacaaga atccaagttg
     9541 acggcaaaga cttagcaaac ttcaaccctg atttgacaga ctactacctt gagtctgtag
     9601 atggaaaagt tccggcagtc acagcaagtg ttagcaacaa tggtctcgct accgtcgttc
     9661 caagcgttcg tgaaggtgag ccagttcgtg tcatcgcgaa agctgaaaat ggcgacatct
     9721 taggagaata ccgtctgcac ttcactaagg ataagagctt actttctcat aaaccagttg
     9781 ctgcggttaa acaagctcgc ttgctacaag taggtcaagc acttgaattg ccgactaagg
     9841 ttccagttta cttcacaggt aaagacggct acgaaacaaa agacctgaca gttgaatggg
     9901 aagaagttcc agcggaaaat ctgacaaaag caggtcaatt tactgttcga ggccgtgtcc
     9961 ttggtagtaa ccttgttgct gaggtcactg tacgagtgac agacaaactt ggtgagactc
    10021 tttcagataa ccctaactat gatgaaaaca gtaaccaggc ctttgcttca gcaaccaatg
    10081 atattgacaa aaactctcat gaccgcgttg actatctcaa tgacggagat cattcagaaa
    10141 atcgtcgttg gacaaactgg tcaccaacac catcttctaa tccagaagta tcagcgggtg
    10201 tgattttccg tgaaaatggt aagattgtag aacggactgt tgcacaagga aaagttcagt
    10261 tctttgcaga tagtggtacg gatgcaccat ctaaactcgt tttagaacgc tatgtcggtc
    10321 cagagtttga agtgccaacc tactattcaa actaccaagc ctacgacgca gaccatccat
    10381 tcaacaatcc agaaaattgg gaagctgttc cttatcgtgc ggataaagac attgcagctg
    10441 gtgatgaaat caacgtaaca tttaaagcta tcaaagccaa agctatgaga tggcgtatgg
    10501 agcgtaaagc agataagagc ggtgttgcga tgattgagat gaccttcctt gcaccaagtg
    10561 aattgcctca agaaagcact caatcaaaga ttcttgtaga tggaaaagaa cttgctgatt
    10621 tcgctgaaaa tcgtcaagac tatcaaatta cctataaagg tcaacggcca aaagtctcag
    10681 ttgaagaaaa caatcaagta gcttcaactg tggtagatag tggagaagat agctttccag
    10741 tacttgttcg cctcgtttca gaaagtggaa aacaagtcaa ggaataccgt atccacttga
    10801 ctaaggaaaa accagtttct gagaagacag ttgctgctgt acaagaagat cttccaaaaa
    10861 tcgaatttgt tgaaaaagat ttggcataca agacagttga gaaaaaagat tcaacactgt
    10921 atctaggtga aactcgtgta gaacaagaag gaaaagttgg aaaagaacgt atctttacag
    10981 cgattaatcc tgatggaagt aaggaagaaa aactccgtga agtggtagaa gttccgacag
    11041 accgcatcgt cttggttgga accaaaccag tagctcaaga agctaaaaaa ccacaagtgt
    11101 cagaaaaagc agatacaaaa ccaattgatt caagtgaagc tagtcaaact aataaagccc
    11161 agttaccaag tacaggtagt gcggcaagcc aagcagcagt agcagcaggt ttaactcttc
    11221 taggtttgag tgcaggatta gtagttacta aaggtaaaaa agaagactag aaattttgat
    11281 accttcttta tcattaaaga ccaaagaaaa actcttgtcc taaatagaag ataaagagcc
    11341 tgagacaaaa tagttctcaa ccacaaaaaa gctagatatt tccaattgtg gaactctagc
    11401 tttttaattt tgagtcattc tcttattgta tagtagattg aaactagaat agtacacttt
    11461 tacttctaaa acattgttag aaatcgattt gattgtcctt atcgatttgt cctgttctta
    11521 tttcatttta ctatatttta ggaggttatt ggccataagt accaatccca tgtcaattct
    11581 cacttgacgc ttccctctca gattacatct cttgtaaccc aaagaagcct ttatctgccc
    11641 aaagacaggt tccacatcaa tcttgcgttg agcgaaaatc tgtctacctt ggggagataa
    11701 aagcgcctga cattctttag ctttcaagtt ttgatagcgt tcgttcatat acagtccctt
    11761 ttgaggggct gattcaggtt cgtcggcgta gtaaaccttg atttcttgtt gaaagtctgt
    11821 ctgtgttttc tgatatttgg tatggtgaaa acgataatac cagccatcag gatgtgtgta
    11881 gctatcctcc ttgtcattat agtgccaatt cgctaagttt ttagctgact ctttataccc
    11941 tctcttttgt ttcttatcaa acatggcata tttaatcaga aggggagtga tttcttcttg
    12001 aaaatagacc tgtatctgtt cttgaagttt ggcggagaat ttctctgtcg ttttcttcca
    12061 cacaaaacta tacttgttgg cattggcttc aatcttagtt ctgtcaataa acaaacaatc
    12121 taaggtcact aactcttcca tttttcaatt tttcgaccag agaaaatccc ttgtgaatag
    12181 gcaaatagaa gagtagatac aagcatttta gggtgataag acgggcgatc aaaggcatga
    12241 taggaggtgt agaagtgacg ttcctccaag gtattcacca ctttttcaat agtaaaaacg
    12301 agatgatctt gtggtaagaa ggaactgatt tctagtggta aagttgtttg atttgtgtta
    12361 tagtgaatat gcatgggata gcctttctaa atggtttatt gggcaattct attttaccaa
    12421 gactatcgta accatgcaaa aaagcaacgg caaatccgtt gcctttttgg atataagaga
    12481 ctattgtccc agactctttt ctttgtataa aattgtatag tgttattgtc tatactattt
    12541 tgtagagaaa gcaaaaatcc ctagaaaatg cttagggatt cgactatttc attaagaaca
    12601 attcaacaat catttctata tacatgataa gggttaataa gaatcctgca cgccagaaga
    12661 atttgataaa tttaggatag taaaagctgc gttttttcaa aaggaagaaa aagacgagaa
    12721 taatggctag gatcgagagg gctagcccca gtctagggag gaaattatgg gtaaaggttt
    12781 tagctgtaat caagtaatac tcaaatacca agactggaaa agccaaatcc gcaaagtttc
    12841 gtcctagttt ttttaatcta aaaagtttgg ttaggataat gcagacgact aaggtcagta
    12901 tcaataataa aatagatgct aatttcatta aaatcatacc catattgtat cataaaaaaa
    12961 ggctaatgga aacgagaaac agaggaattt ataggaaagt caggcttttg agattgtggg
    13021 tgatttttgt tataatgaaa gtcatgaaat cttataatac cttgaatgat tattatcgaa
    13081 aactctttgg agaaaagact tttaaagtcc ctattgatgc gggatttgac tgtcccaatc
    13141 gtgatggaac tgtggctcat ggaggctgta ctttttgtac ggtttcaggt tctggagatg
    13201 ctattgtagc cccagatgcg cctatccgtg agcaatttta taaggaaatt gactttatgc
    13261 accgtaagtg gccggatgtt cagaagtatc tggtttattt tcaaaatttt accaacaccc
    13321 atgaaaaggt ggaagttgtt cgagagcgtt atgaacaggc tatcaatgag ccaggtgtgg
    13381 taggtatcaa tattggaacg cgcccagact gtttaccaga cgaaaccatc gaatatttgg
    13441 ctgagttatc ggagtgcatg catgtgacgg ttgaattggg cttgcaaact acttatgaag
    13501 caacctctga cctgattaac cgtgcgcatt cttatgaatt gtaggtggaa acggtcaagc
    13561 gtttgagaaa gtatccaaaa attgagattg tttcccattt gatcaatggt ttgcctggtg
    13621 aaacccatga gatgatggtt gaaaatgtcc gccgttgtgt cacgaataac gatattcaag
    13681 ggattaaact gcatttgctc catcttatga caaatactcg tatgcaacga gattaccatg
    13741 agggacgttt acaattgatg agtcaggacg aatatgtcag ggtcatctgt gaccaactgg
    13801 aaatcattcc caaacatatc gtcatccatc gaattacagg agatgcgcct agggatatgc
    13861 tgcttgggct tatgtggagc ctcaataaat gggaagctct caacagcatt gagatggaga
    13921 tgcgacgtcg tggaagtgtt caaggatgca aggctgtaaa acaggagttt gaaaatgaaa
    13981 agaccacttg agatggcaca tgattttttg gctgaggtcg tgacaaaaga ggatgtcgta
    14041 gtggatgcga ctatgggaaa tggtcatgac acgctttttt tagccaagct agccaagcaa
    14101 gtctatgcct ttgatattca gaagcaagcc ttggaaaaga cccaagagcg tttgcatcag
    14161 gctggcttga caaatgccca gttaatcttg caaggccatg agacactgga ccagtttgtg
    14221 acaaaagcta aggcagggat ttttaatttg ggctatttgc cgtcagctga taagtctgtc
    14281 atcacccgac cgcagacaac gattgaggca ttagaaaagc tatgtggctt acttgtcaaa
    14341 ggtggacgaa ttgctattat gatttactat ggtcatgaag gaggcgacct cgagagagat
    14401 gctgtcttgg attttgtgat ccagttgaac caacaagagt acacagctgc catttaccga
    14461 actttaaacc aagtcaacaa cccgccgttt ttagtgatga ttgaaaaatt agagagatac
    14521 agacatggat aaacaatacc tacacgaaaa gctggatgcc atgcgccaga attttgttga
    14581 atcaacccac cacgaacgag cggtgggcgt gctagaccaa gcgcatatga gcaaaaaaat
    14641 gcttaaaatc aagaaaaaat tagttgctct tgaaatggaa cggtgccaga gaaaaattga
    14701 gcacaaagac tgttccaaga ttgaccaaaa aatcaaagag cagaaggaga tatttgaatc
    14761 ctgttgtaaa aaagattaag gaggacgtgc gtggaattac tgatttacct catcctattt
    14821 ttactggttt tgattgtctc aagtacaacc aataagctcc tgcccttttt gcctctccct
    14881 ttggtgcaga ttcttttggg aattgtgatt ggtctctttt tacccaatac tgactttcac
    14941 cttaatacgg agttgttttt ggcactggtt atcggaccct tgcttttccg agaggctgaa
    15001 gaagcagatg ttacggctat tttaaaacac tggcgaatca ttgtttatct catatttcca
    15061 gtgattttta tctcgaccct gagtttgggt ggcttggccc atcttctttg gttcagcctt
    15121 cccttggcag cttgcttggc tgttggtgca gcccttggtc ctacggactt ggtggccttt
    15181 gcctctcttt cggagcgttt tagctttcct aagcgcgtgt ccaatattct taagggcgaa
    15241 ggactcttga atgatgcttc tggtttggtg gcttttcagg tagctttgac agcttggaca
    15301 actggagctt tttctctggg gcaagctagc agttcgctca tcttttcaat cctaggcggt
    15361 tttttaattg gatttttaac agccatgacc aaccgcttcc tccatacctt cttgctaagt
    15421 gtgcgcgcaa cggatattgc cagtgaactt ttattagaat tgagtttgcc tctagtgacc
    15481 ttctttctgg cagaagaagt ccatgtttca gggattattg ccgtcgtagt tgctggaatt
    15541 ttaaaggcaa gtcgcttcaa gaaaatcacg ctcctcgaag cccaagtgga tacggtgacc
    15601 gagacggtct ggcatacagt gacctttatg ctcaacggtt ctgtctttgt gattttaggg
    15661 atggagttgg aaatgatagc agaacctatc ttgaccaatc caatctataa tcctctactt
    15721 ttattgctat ctctcatcgc ccttaccttt gtcctctttg tcattcgttt tattatgatc
    15781 tatggctatt atgcctatag aacccgacgc ctcaagaaaa agctaaataa gtatatgaag
    15841 gacatgtttc tcttgacctt ttcaggtgtt aagggaacgg tgtcgattgc tacgattctc
    15901 ttgataccaa gtaatctaga acaggagtat cctctcttgc ttttccttgt tgcaggtgtg
    15961 acgcttgtca gctttttaac aggtctcttg gtcttgcctc atctttctga tgaagaggaa
    16021 gaaagcaagg attatctcat gcatatcgcc attttgaatg aagtaacgct agagttggaa
    16081 aaagagttgg aagacaccag aaataaactt cccctctatg cggctattga caattatcat
    16141 ggacgtattg aaaatctcat tttaagccaa gaaaaccagg atgatcaaga agattgggct
    16201 gctttgaaac tcttgattct tagtattgaa agtgatggtt tggagcaggc ctatgaagag
    16261 gggaacatta gcaatcgtgc ttaccgagtt taccaacgtt atctgaaaaa tatagaacaa
    16321 ggaatcaatc gtaaacttgc ctcaagactg acctattatt tccttgtttc tttgcggatt
    16381 ttacgttttc tccttcatga agtttttact ctcggaaaga ccttccgtag ctggaagaac
    16441 aaggagcaaa gccgtctccg tgctcttgat tatgaccaaa ttgcagagct ctatcttgcc
    16501 aatacagaga tgattattga aagtttggaa aacctgaagg gagtctacag acgctctttg
    16561 attagtttta tgcaggagtc tcgtcttcga gaaacagcta ttatcagcag tggtgccttt
    16621 gtcgaacggg ttatcaatcg tgtcaaaccc aacaatatcg atgaaatgct gagaggctat
    16681 tatctggagc gcaagttgat tttcgaatac gaagaaaaac gattgattac gactaagtat
    16741 gccaagaaat tacgacaaaa tgtaaataac ttagagaact attccttgaa ggaagctgcc
    16801 aataccctac cgtatgatat ggtggaattg gtaagaagaa attagttaat actcttcgaa
    16861 aatctcttca aaccacgtca gcgtcgcctt accgtatgta tgtgactgac ttcgtcagtc
    16921 ttatctacaa cctcaaaaca gtgttttgag cagcttgcgg ctagctttct agtttgctct
    16981 ttgattttca ttgagtataa gattataaat gaaggagtat gacatgaaaa agtggtggaa
    17041 agagctgata gacaaacctt tattaaaagc ttttttgcat tattatcaag catcagatag
    17101 tgagttgacc agtgtcgcag tagcctacta ttggttgatt tcgattttcc ccctgctttt
    17161 ggtggtggtc aatatcctcc cctattttca gattcctgtg ggagaatttt tgggctttat
    17221 gaaagacgtc ctgcccccaa gcctctatga aggtgtggaa aaaatagcca gagaagtctt
    17281 gacgcaacct tcaacaggtt tattgagttt ttctgtttta tcggcgttat ggagtttttc
    17341 aaaatctatg aatttcttgc aaaaagcttt taacaaggct tatggcgtcg aaaagagtcg
    17401 tggacttatt tcccatcaga tgctaagtct cctagtcagt tttggcttgc agctcctctt
    17461 tgcgtttgcc ttgtttttga tcttatttgg tcagatgatt ttggctttac ttgctcatta
    17521 ttggacaaaa gatggcatta tctatcaggc tttacaaggg ttggcaggtc ctctgattta
    17581 cgccctcctc tttgctatct tggtcatgct ttattatttt cttcccaatc tatctaacag
    17641 gaaaattagc tataccctac caggcagtgc ctttgttctc ttagtgattt taggtttact
    17701 aactctgttc tctagttatc tcaattacta tgtccatcat ttggtggatg tccgaatttt
    17761 aggttccgtt cttcttgttg ttatgatgtt ttggtttatc ttgattgcta aaattgttat
    17821 cctaggcgcg gtgatcaatg ccagtatgca gagtttgaaa gatccggtct ttaaaaaaga
    17881 ctaactattt atccattaca aaagcgcata gtatcaagat tctataaagc cttgatacta
    17941 tgcgcttttg tgctttgaca gttttttgta tagtttttgt ccaagatttc taaatcatct
    18001 gattaaattc ctattttccc ctatcattgt ccctgttttt tcgaaaatgg aaggagtata
    18061 ataaatctat caataaaatg ataggaggaa ctttatgggt catattttct tttttctaag
    18121 tgtctttttg gcagggattc tatccttctt ttctccttgt atcttacctt tgttaccggt
    18181 ctatacagga gtgttactag atgataagga tggtgctcag gcttctagcg gcaaattttc
    18241 aatctcagtt actagtttat tacgaactct ggcctttata gcaggaattt cctttatatt
    18301 tattttgttg ggctatggag ctggtttttt aggcgatttg ctttatgctt cttggttcca
    18361 atatcttact ggggcaatta ttatccttct tggtttgcac caaatggaga ttctacactt
    18421 taaggggctt tataaggaaa agaggctaca actgcaagga caggggcaaa atggtaaggg
    18481 ctatagtcag gcatttttat tgggcttgac ctttagtttt gcttggacgc cttgcgtggg
    18541 gccggttctg gggtctgttt tggccttggc ggcttcaggt ggttcaggag cttggcaggg
    18601 agctggtctc atgttggtgt atacgctggg cttggcgcta ccattcttgc ttctagctct
    18661 gacctctagt tatgttttga aacatttccg aaaacttcat ccctatctcg gaatcctcaa
    18721 aaaagtgggt ggttttctca ttattgtgat gggattcttg gttctgtttg gaaatgcttc
    18781 aattttaagt caattatttg aataaaatgg aaaggaatat caatatgaaa aaatggcaaa
    18841 catgtgttct tggagcaggt tcgctccttt gtttgacggc ttgttcaggc aagtccgtga
    18901 ctagtgaaca ccaaacgaaa gatgaaatga agacggagca gacagctagt aaaacaagcg
    18961 cagctaaagg gaaagaggtg gctgattttg aattgatggg agtagatggc aagacctacc
    19021 gtttatctga ttacaagggc aagaaagtct atctcaaatt ctgggcttct tggtgttcta
    19081 tctgtctggc tagtcttcca gatacggatg aaattgctaa agaagctggt gatgactatg
    19141 tggtcttgac agtagtgtca ccaggacata agggagagca atctgaagcg gactttaaga
    19201 attggtataa gggattggat tataaaaatc tcccagttct agttgaccca tcaggcaaac
    19261 ttttggaaac ttatggtgtc cgttcttacc caacccaagc ctttatagac aaagaaggca
    19321 agctggtcaa aacacatcca ggattcatgg aaaaagatgc aattttgcaa actttgaagg
    19381 aattagccta ggaggcgtct tatgaatgat aagttaaaaa tcttcttgtt gctaggagta
    19441 ttttttctag ccataaccgg tttctatgtt ctattgatac gaaatgcagg gcagacagat
    19501 gcctcgcaaa ttgaaaaggc ggcagttagc caaggaggaa aagcagtgaa aaaaacagaa
    19561 attagtaaag acgcagactt gcacgaaatt tatctagctg gaggttgttt ctggggagtg
    19621 gaggaatatt tctcacgcgt tcccggggtg acggatgccg tttcaggcta tgcaaatggt
    19681 agaggagaaa caaccaagta cgaattgatt aaccaaacag gtcatgcaga aaccgtccat
    19741 gtcacctatg atgccaagca aatttctctc aaggaaatcc tgcttcacta tttccgcatt
    19801 atcaatccaa ccagcaaaaa taaacaagga aatgatgtgg ggacccagta ccgtactggt
    19861 gtttattaca cagatgacaa ggatttggaa gtgattaacc aagtctttga tgaggtggct
    19921 aagaaatacg atcaacctct agcagttgaa aaggaaaact tgaagaattt tgtggtggct
    19981 gaggattacc atcaagacta tctcaagaaa aatccaaatg gctactgcca tatcaatgtt
    20041 aatcaggcgg cctatcctgt cattgatgcc agcaaatatc caaaaccaag tgatgaggaa
    20101 ttgaaaaaga ccctgtcacc tgaggagtat gcagttaccc aggaaaatca aacagaacga
    20161 gctttctcaa accgttactg ggataaattt gaatccggta tctatgtgga tatagcaact
    20221 ggagaacctc tcttttcatc aaaggacaag tttgagtctg gttgtggctg gcctagtttt
    20281 acccaaccca tcagtccaga tgttgtcacc tacaaggaag ataagtccta caatatgacg
    20341 cgtatggaag tgcggagccg agtaggagat tctcaccttg gccatgtctt tacggatggt
    20401 ccacaggaca agggcggctt acgttactgt atcaatagcc tctctatccg ctttattccc
    20461 aaagaccaaa tggaagaaaa aggctacgct tatttactag attatgttga ttaatactct
    20521 tcgaaaatct cttcaaacca cgtcagcgtc gccttaccgt atgtagggtt actgacttcg
    20581 tcagttttat ctacagcctc aaagctatac tttgagctga cttcgtcagt tttatctaca
    20641 gcctcaaagc tatactttga gctgacttcg tcagttttat ctacaacctc aaagctatac
    20701 tttgagctga cttcgtcagt tttatctaca acctcaaagc ggtactttga gaaacctgtg
    20761 gctatcttcc tagtttactt tttgattttt attgagtata agaggtcttt cctaagcagt
    20821 tagaggagaa ttttgctata ctgatactag taagtggtaa aggagcagat catgacctac
    20881 acaatcttaa tcgtagaaga tgaatatctg gtaagacaag gtttgactaa actggtcaat
    20941 gtagcagcct acgatatgga aatcatcggt caggctgaaa atggaaggca ggcttgggaa
    21001 ttgatccaaa agcaggtgcc agatatcatt ttaaccgata tcaacatgcc tcatctaaat
    21061 ggcatccagt tggccagtct ggtacgagaa acctatcctc aggttcattt ggtcttttta
    21121 acaggttacg atgattttga ttatgccttg tctgctgtca aactaggtgt ggacgactac
    21181 ctgctcaaac ccttttctcg tcaggatatt gaggaaatgt tggggaaaat caaacaaaaa
    21241 ctagacaagg aagagaaaga agagcagtta caagatttat tgactaatag gtttgaagga
    21301 aacatggccc agaaaatcca gtctcatctg gctgatagcc aatttagttt aaagtcttta
    21361 gccagtgact taggttttag tccgacctat ctgagttcct tgattaagaa agagttgggc
    21421 ttgccttttc aggattatct ggtgagagaa cgtgttaaac aagccaagct cttgctttta
    21481 actacagatc tgaagattta tgagatcgca gagaaggttg gttttgaaga tatgaactat
    21541 tttacccaac gttttaagca gattgcaggt gtgacacctc gtcagtttaa gaagggagaa
    21601 gaccgatgaa gcgttcttct cttttagtta gaatggttat ttccatcttt ctggtctttc
    21661 tcattctcct agctctggtt ggaactttct actatcaatc aagttcttca gccattgagg
    21721 ccaccattga gggcaacagc caaacgacca tcagccagac tagccacttt attcagtctt
    21781 atatcaaaaa actagaaacc acctcgactg gtttgaccca gcagacggat gttctggcct
    21841 atgctgagaa tcccagtcaa gacaaggtcg agggaatccg agatttgttt ttgaccatct
    21901 tgaagtcaga taaggacttg aaaactgttg tgctggtgac caaatctggt caggtcattt
    21961 ctacagatga cagtgtgcag atgaaaactt cctctgatat gatggctgag gattggtacc
    22021 aaaaggctat tcatcaggga gctatgcctg ttttgactcc agctcgtaaa tcagatagtc
    22081 agtgggtcat ttctgtcact caagaacttg ttgatgcaaa gggagccaat cttggtgtgc
    22141 ttcgtttgga tatttcttat gaaactctgg aagcctatct caatcaactc cagttggggc
    22201 agcagggctt tgccttcatt atcaatgaaa accatgaatt tgtctaccat cctcaacaca
    22261 cagtttatag ttcgtctagc aaaatggagg ctatgaaacc ctacatcgag acaggtcagg
    22321 gttatactcc tggtcacaaa tcctacgtca gtcaagagaa gattgcagga actgattgga
    22381 cggtacttgg cgtgtcatca ttggaaaagt tagaccaggt tcggagtcag ctcttgtgga
    22441 ccttgcttgg ggccagtgtc acatctcttc ttgtctgtct ctgcttagtg tggttcagtc
    22501 ttaaacgctg gattgctcct ttgaaggatt tgagagaaac catgttggaa attgcttctg
    22561 gtgctcaaaa tcttcgtgcc aaggaagttg gtgcctatga actgagagaa gtaactcgcc
    22621 aatttaatgc catgttggat cagattgatc agttgatggt agctattcgt agccaggaag
    22681 aaacgacccg tcagtaccaa cttcaagccc tttcgagcca gattaatcca catttcctct
    22741 ataacacttt ggacaccatc atctggatgg ctgaatttca tgatagtcag cgagtggtgc
    22801 aggtgaccaa gtccttggca acctatttcc gcttggcgct caatcaaggc aaggacttga
    22861 tttgtctctc tgacgaaatc aatcatgtcc gccagtatct ctttatccag aaacaacgct
    22921 atggagataa gctggaatac gaaattaatg aaaatgttgc ctttgataat ttagtcttac
    22981 ccaagctggt cctacaaccc cttgtagaaa atgctcttta ccatggcatt aaggaaaagg
    23041 aaggtcaggg ccatattaaa ctttctgtcc agaaacagga ttcgggattg gtcatccgta
    23101 ttgaggatga tggcgttggc ttccaagatg ctggtgatag tagtcaaagt caactcaaac
    23161 gtgggggagt tggtcttcaa aatgtcgatc aacggctcaa acttcatttt ggagccaatt
    23221 accagatgaa gattgattct agaccccaaa aagggacgaa agttgaaata tatataaata
    23281 gaatagaaac tagctaactc ccagtcaatt ctgggagttt tatgcgtaat tgggcaaact
    23341 ttataggttg tctatcttgc taaaaacaag aaaaaacggt atgatagaag agtgacaaca
    23401 gaggtaacag agatgaacga gacacagatt caaagagaaa cacgtcaggt agtggaagat
    23461 gttttagaaa agaccaattt gaagcagggg gtcctttttg ttttaggtct atcttctagt
    23521 gaggtgctag gtggtcagat tgggaaagaa tccagtcaag agattggtga gctgatagtg
    23581 gagactatct tgggtattct aggtagtaga ggtatccatt tagcagttca aggttgtgag
    23641 catgtcaatc gggctctcgt tgttgaacgt caggtggcag agcagtttga tctggaaatt
    23701 gtcagtgtcc atcctactct tcatgcagga ggttcggggc agttggcagc ctttaagttt
    23761 atgcaggatc cagttgaggt tgaatttatc aaggctcatg ctggattgga tatcggagac
    23821 actgcaattg gcatgcatgt caagcatgtt caggttccga ttcgccctat tttgagagag
    23881 attggtcatg cccatgtaac ggcactggct agtcgtccaa aattaatcgg aggtgcgcgt
    23941 gcgcactatc cgcaagacgc tattagaaag tcttgagaat gaataaagga gataaaagtg
    24001 tttaaaaaag accgtttttc aattcgtaag attaagggag ttgtaggctc tgtatttctt
    24061 ggaagccttt tgatggctcc ttctgtagtg gacgcagcca cctatcacta tgtaaataaa
    24121 gagattattt cacaagaagc taaagattta attcagacag gaaagcctga caggaatgaa
    24181 gttgtatatg gtttggtgta tcaaaaagat cagttgcctc aaacagggac agaagcatct
    24241 gttttgacag cttttggttt gctgactgtt gggagcttgc ttttaatcta caagagaaag
    24301 aaaattgcta gcgtctttct agttggagct atgggattgg tagttcttcc tagtgcagag
    24361 gctgtagacc cagttgcgac cctagcgctg gctagtcgag agggtgttgt tgaaatggat
    24421 ggctatcgct atgttggtta tctatcaggt gacatcctca aaacgcttgg cttggacact
    24481 gttttagaag aaacctcagc aaaacctgga gaggtgactg tagtcgaagt tgagactcct
    24541 caatcaacaa caaatcagga gcaagctagg acagaaaacc aagtagtaga gacagaggaa
    24601 gctccaaaag aagaagcacc taaaacagaa gaaagtccaa aggaagaacc aaaatcggag
    24661 gtaaaaccta ctgacgacac ccttcctaaa gtagaagagg ggaaagaaga ttcagcagaa
    24721 ccagctccag ttgaagaagt aggtggagaa gttgagtcaa aatcagagga aaaagtagca
    24781 gttaagccag aaagtcaacc atcagacaaa ccagctgagg aatcaaaagt tgaacaagca
    24841 ggtgaaccag tcgcgccaag agaagacgaa aaggcaccag tcgagccaga aaagcaacca
    24901 gaagctcctg aagaagagaa ggctgtagag gaaacaccga aacaagaaga tacacagcca
    24961 gaagtagtag aaacaaaaga tgaggctgct aatcaacctg ttgaagaacc aaaagttgaa
    25021 acgcctgctg tagaaaaaca aacggaacca acagaggaac caaaagttga acaagtaggt
    25081 gaaccagtcg agccaagaga agacgaaaag gcaccagtct ctccagaaaa gcaaccagaa
    25141 gctcctgaag aagagaagac tgcggaagaa acaccgaaac aagaagataa aataaagggt
    25201 attggtacta aagaaccagt tgataaaagt gagttaaata atcaaattga taaagctagt
    25261 tcagtttctc ctactgatta ttctacagca agttacaatg ctcttggacc tgttttagaa
    25321 actgcaaaag gtgtctatgc ttcagagcct gtaaaacagc ctgaggtaaa tagcgagaca
    25381 aaagctgaaa aagttgcagc taatacagat gctaaacaaa gtgaagtaaa ttctgagact
    25441 gcaagtctta aaacagcaat ttctggatta aatacagata aagttgaact cgaaaatcaa
    25501 cttaagattg cacagggaaa aactgaaact gatttcagca tggaatcttg gacggttttg
    25561 agtacagcta aaaataaagc tcaggaagta aaagataacg gaacagcgac ccaagaacaa
    25621 ataaacgaag cagagaaaag tttgaaaaca gcccttgctg acttatctgt tgacaaaaca
    25681 gctttgggaa gcgctatcga tacagctacc aagaaaaata aagagaacta tacaaatcaa
    25741 acatgggcag agttagaaac agttcttaca gcagcaaaat ctgtaaacac taacgagtct
    25801 aaacaaagtg aagttaatga agctgttgaa aaattaactg caactattga aaaattggtt
    25861 gaattgtctg aaaagccacg tttgactcta tctattgaaa agagagacat agatcgaaaa
    25921 gttacggtca cgtatacttt ggaaaatcca gcaaatactc aaataaaatc aataactgca
    25981 actctgaaaa aaggagagga agttgttaag gattttgttt taacagaaga aaatcttaaa
    26041 actaatcatt taactgcttt gtttgaaaag ctagattact acaaagaata cactctatct
    26101 acggatatgg tatataacag aggaaatgat gatgagacgg agagtatttc agaagaacta
    26161 attcagctga atcttaagaa actagaattg aaagatatcc agacagtctc tctgatgaaa
    26221 tttgaaaatg gtcaagaaag tcaggttaca cacttgtctg ataaaccgac cgatttgagt
    26281 aaattatact taaaagttac atcatcaacg tctaaggatg ctgtgttagc cgttagctct
    26341 atagaagaag agattgttga aaataaaaaa atctttaaaa ttcatgcaga tacaccagaa
    26401 ttagtagttc gtaagaaaga tggcagtctt tctaagggat ttgactatta catggagaga
    26461 gttatccctc atgatggtga tatttattat gattttaaag acttgatttc agctatgaca
    26521 tctaatccta caggtacgtt tattttaggg agagatattt cctcgcgtaa tgtaaaaccg
    26581 gatggaaatg gaaaatctta tatcaaaggt gaatttaaag gaaaactact tggtacaaat
    26641 gataatgtta gacactcgat ctttgactta gaatatccat tgtttgatac tataaaatca
    26701 ggagtcgtga aagatattga tttcaagcat gtcaatatgg tattcccaga tagcaatcaa
    26761 ggagataacg tagcaacaat tgcaagagta atcaaagata agacaaaaat tgagaatgtt
    26821 aatgttgaag gttatctcga gggtcgagat catgtagctg gtttagttaa taatcttgaa
    26881 ggtaattccg aaattgaaaa tatatccttt acaggaaaga ttaagtctaa aggtggtaat
    26941 tctattacag caggtattgc aggtagaaat atcttatctc gtgttaaacg tgcctatgta
    27001 aatgcaaata ttgaagtcct tggttcaacg aattcgagta tgcttgttgc agtaaatggt
    27061 acaacattga atgctagtgg aggttgggga gcctggggaa gacttactga gtccgttgct
    27121 aaagggacgt tagaaattaa acgcagtgga caagctggtg gagttactgc aacagtctgg
    27181 ccttatggag caattgataa ggttgtcagc tatgcaaaag ttaccaaagg aaaagaatta
    27241 tttgggtcag atggtgattt aaataataat tggtttatgc aaaaaattaa taatattttt
    27301 ggtgttcaag gtatcagctc aggtgactca gggaatgatt ctaagtttaa gcgtatttcc
    27361 gaggaagaag caaagcaaaa ggttgcctct tataatatta cagctcccaa cttgatgagc
    27421 gactcaagtc ttttggtaga cagactcaat gaaagttgga aaaatactga tcaatttgaa
    27481 agtatacagg attatcaatc ccaaaaccaa ttaatttacc agaacttgac taaatttact
    27541 ccttattaca ataaagaatt tattgttcat gaaggaaatg ctctcactcc tgaacaggaa
    27601 atattgaaga ctaagaaaat caaatcgatt gtagggttga aaggaacaga gttcgttgta
    27661 gatggcagtg atattgatac tatcatgctt cattttgagg atggtagtca gaagcgatat
    27721 aaggtaacct ctactggtaa gttttcgata actaatttac cggaatatca ggttgaagat
    27781 ttaaatgtgg tgtatacatc agaacatata gtacatccac tagattcgtc actgataaat
    27841 aaccttgttg aagaattgaa aaaagtagaa ttgtatacag agtctactta ccaagttcta
    27901 ggtattgata aagataatgc taacaaattg aatagaacta agagattatt cttagatgag
    27961 tcattagatg ctgttaaaac acaattgcct acctttgtaa aaacaatgtt tgaaaatgag
    28021 tggttgcaca tcaatggaga atctagtgga gctgtcgctg ctttacgaca aaaaatcatg
    28081 gataataaga cggctatatt gcttgctttg acttatatca atcgttatta tgatgttaaa
    28141 ttctcagact ataatattaa aaaactgatg ctctttaaac caactttcca tggtgaaaag
    28201 attgatctat tagatcgatt aatccgacta ggatcatctg gagaaaatag attaaaaggt
    28261 tcagaaaatg cagaaacgtt caagcagtta tttgctagcg aaacgaagca gaaggatttg
    28321 gtgacttatt tagactacaa tcgctctttg ttgactaatt atcaaacaac aggggaatgg
    28381 tttaaagaaa cgactaagga ttatattcaa tttgaggaac gtccatcctt agtagaggag
    28441 attaaagatg ccaaatatcg agtttacgat aatttaaccg caccgtatta tcaaggatat
    28501 atcctacctc tcttaacgtt gaagaatact catttagcaa ttttatcgaa ctatagtact
    28561 atgacatttg tcagccgtga aaaacgccct aattggaaaa atgaagattt tgataagtgg
    28621 gtgaaatatg tagcaactgc tcatagaaac catgttgata cttggtataa gattttacct
    28681 gataatatca aaggtaaaat ggttaaagag aatgttactg ctgtttggga aggattgagc
    28741 atcccaggaa gtgaatgggt agaccagaat gctgtagata gaaaaggaag agattacgcc
    28801 ccagctcgag aattctttaa tcttgtaggt ggtcctatgg gaggttggta tgcttaccat
    28861 ggttatggtg cgcatgctgg aggaagaaat cgggttaatt atgaggtgtt tgatgtatta
    28921 agtgagtatg gtatatcggt ctttactcat gaattgactc acgttaatga tacttggatt
    28981 tacttaggag ggtatggtag acgtgaaaat atgggacctg aagcttatgc tcagggatta
    29041 ttccagtctc ctgttccagg tcagccgggt tggggggcgc ttggtctcaa tatggcattt
    29101 gaaagaaaaa atgatggtga tctaatttat aatgcctccc caactcaatt tgaaaatagg
    29161 aaagagttag atagttatat gaaaaattat aacgatactc tcatgatggt ggactacttg
    29221 gaaggagatg cagttatctc taaaggtaaa gaagctatta ctaaatggtt taaaaaagtt
    29281 gaacctaagg ttgttagtca aacagctcag tatgatacgg ttcgtcagtt gactgctgaa
    29341 gagaaagaaa aactttctgt ttctagtgtt gatgatttag ttgatcaagg attaatgtca
    29401 gacagagctg taggaaataa tacctataat ccagctgatt ttgaaacaag ttatattgcg
    29461 atagattata tgactggtat ttatggtgga ggaaagaata gtgtaggttc accaggtgcc
    29521 ttgatgttta aacataatac tttccgtatg tggggctact atggttttga agaaggtgtt
    29581 ctaggatatg cttctaataa atttaagcaa gcatctagag atgaaggtca tgctggactt
    29641 agtgataact ttattatttc taagatttcc aaaggggaat ttttaactat ggaagcattt
    29701 aaaaagggat attttaagaa agtagtagaa gagttaaaaa ctaaaggtat tcgtcctgtt
    29761 actatcaatc aaaaaacata tagcaccttt gaagaattgc aagaaggatt taagcaggct
    29821 gttgaaagag atttgaagaa aaatcagtta gatgagaggg aaacaagaaa cttcaaattc
    29881 caagttttca gacaacttct tcaacaaaca gatagcttta aaacttctat ctttagataa
    29941 tcgaaatctc tcatctgctt tgcggatgag ggttttctta ttttatgcta tacttaaggt
    30001 atgcatagaa aaacagtgat tgattttagg actttagggg agagatatac ctttacccag
    30061 cctattaaag agttgaaaac gagaaatgta gaagaagtgg cagatttgct ggcacaagtg
    30121 gaaagctacc aagagcaagg ttattatgtg gtggggtatg tcagctacga ggctgcacct
    30181 gcttttgagg aaaaattagc agttcacaag gatcccctac tgggagagta cctgctttat
    30241 tttactgttc atgatagcgt agaaacttcc cctattcctc tgacttatga ggatattgat
    30301 ttgccctcaa attggcagga agtaacgtct gcagcagact atgaaaaggc cattgcccag
    30361 atacaccatc acttgaggca gggagacacc tatcaggtca actacaccgt ccaactcaag
    30421 caaaagttaa gtgccaatcc ttttgccatc tacaatcgta tggtggtaga gcaggaggcg
    30481 ggctacaatg cctatgtgga acatgacgag atggcagtga tttccatgag cccagagctc
    30541 ttttttgagc aaaatgatcg cgagttgaca acacgaccaa tgaaggggac gactcagcgt
    30601 ggggtaactg accaagaaga tcttgaacag gccagttggt tggaacagga tcccaaaaat
    30661 cgctctgaaa atatgatgat tgtggacctc ttgcgcaatg atatgaaccg tatttctgaa
    30721 gttgggagcg agcacgtgga gcgtctgtgc caggtagagc agtattcaac tgtttggcag
    30781 atgacttcga ccatcaagag tcagttgcga gaggatgtgg accttgttga aatcttccgc
    30841 tcactctttc cttgtggttc cataacgggt gcaccgaaaa ttgcgacaat ggagattatc
    30901 aagaacttgg agcctcaacc gcgtggagtc tactgtggaa cgattggtct cttgcttcca
    30961 aatggacgac ggatttttaa tgtggccatt cgtaccattc aacttcacaa aggtcaagcc
    31021 atttatggag ttggcggagg gattacttgg gatagcacat gggaatctga ataccgagag
    31081 gttcatcaaa aggcagcagt tctttatcgt aaacaagctc gcttccaact gattacgaca
    31141 ggggaaatca gccagaagaa cctgctgttt gaagatcaac atctggaaag actgagaaaa
    31201 gctagtcgtt attttgcctt tccttttgat gcagaagact tgggacacaa gattgaggaa
    31261 gagtgtcagg attgtgaagc taatcaagat taccgcttgc gaatcagcct tagcaaatct
    31321 ggagagatag aagtcaatcg tcaagtatta acccctctca gtacaagctt ttgtcaggcc
    31381 caagtctgcc ttcaggaagc tgctttgaat caatccttta cctactttaa aaccactcac
    31441 cgaccgcatt tgagcctagg agaacaagag aagatttacc acaataagtc aggagaactg
    31501 cttgaaacct ctataggaaa tttggttctg aaaatcgctg gaaaactcta cacaccgcct
    31561 atccgacttg gaatcttgcc aggaatttac cgtcagcatt tgctagaaac aggacaggta
    31621 gaagagaaag tcttgacctt ggcagattta acccaagcag aagctattta cggctgtaat
    31681 gcagtgagag gcttgtatga actaagcctt gaggagaact agatgaaatt gatatttttg
    31741 catgggttag ggcagtcagc agagtcttgg aaagaagttc ggaatctact gacagattat
    31801 ccttctgagg ctatcgagtt atttccttct ggagttagta actatcaaca agctaaagag
    31861 cgagtttatc agcacctagc tcaagagaca gaaccttttg ttttgattgg attgtcctta
    31921 ggtgctgccc tgacactaga actttccagt tacgatttac caaatcttcg agctttgatt
    31981 ctgtcaggat gcccgcttaa attagcaggg aatatcctat tttatcttca gttgctgatt
    32041 tttaaactac ttcccaaaag agtatttgaa aaacagggag cagacaagac tcttatggtc
    32101 ggagtttctg aggaattgaa gacacttgat ttaacggata tagcagggac ttgctcctat
    32161 ccaaccttac taatttgtgg cagcaaagat aaaccgaatc ttagttttat gaaagctctc
    32221 catagactgt taacagattc gcagtttcag atcattcctg atggccctca tgtcttgaat
    32281 aaggcaaaac caaaggagtt tgtagaaaag accagaagtt tccttgaatt gctgaaataa
    32341 gtttgataaa ataatattga aatcgatatc ataattatgg gagaaagaaa tgaataaacg
    32401 tctattttta aaaatgagtc tggtgacgtt gccaatttta gccttgtttt cacaaccagt
    32461 tttggcggaa gaaaacatcc atttttcgag ctgtaaggaa gcttgggcga atggctattc
    32521 ggatattcac gagagagaac ctggttattc tgccaagtta gaccgtgatc atgatggtgt
    32581 ggcttgcgaa ttgaaaaatg ctcctaaggg tgcttttaaa gcaaaacagt caacggctat
    32641 tcaaatcaac acaagttcag caacaacaag tggttgggtt aagcaggacg gcgcttggta
    32701 ctactttgat ggaaatggaa atctagtgaa aaatgcatgg cagggaagct attacctgaa
    32761 agctgatggt aaaatggcac agagtgaatg gatttatgac tcttcttatc aagcttggta
    32821 ttatttgaaa tcagatggtt cttatgcaaa aaatgcatgg caaggagctt attaccttaa
    32881 atcaaacggt aaaatggcac aaggtgagtg ggtttatgat tcttcttacc aagcatggta
    32941 ttacttgaaa tcagatggtt catatgctcg caatgcatgg caaggaaact actatttgaa
    33001 atcagatggt aaaatggcta aaggtgaatg ggtttatgat gccacctatc aagcttggta
    33061 ttatttgaca tcagatggtt cttatgctta cagtacatgg caaggaaatt actatctaaa
    33121 atcggatggt aaaatggctg tcaatgaatg ggttgatggt ggacgttatt atgttggcgc
    33181 tgacggagtt tggaaggaag gtcaagcaag tacagtttct tctagtaatg atagcaatag
    33241 tgaatattct gctgctttag gaaaggcaaa aagttataat tcgttattcc acatgtcaaa
    33301 aaaacgtatg tatagacaat taacttctga ttttgataaa ttttcaaatg atgcagctca
    33361 atatgccatt gatcatttag atgattaaca gatactggta ggcgaaaaaa ttccttataa
    33421 ttcatttacg caaacctttg cataagtgaa tggattttga tatactagta ctgtaagcat
    33481 tttcaattac tacacaatga aaaaggagaa tatgatgagt caaaagatta ttgggattga
    33541 ccttggtgga acttctatca aatttgcaat cttaacaaca gcaggagaaa tccaaggaaa
    33601 atggtcaatc aagaccaaca ttttggatga gggaagtcat atcgttgatg atatgattga
    33661 gtctattcaa catcgtttgg acttgcttgg attggcagca gcggacttcc aaggcattgg
    33721 aatgggatca ccaggtgtgg ttgaccgtga caaagggact gttatcggtg cctacaactt
    33781 gaactggaaa acccttcaac caatcaaaca aaagattgaa aaagctttgg gcattccatt
    33841 tttcatcgat aatgatgcca acgtagcagc tcttggtgag cgctggatgg gtgctggaga
    33901 taaccaacca gacgttgtct ttatgacact cggtactggt gttggtggtg gtattgtcgc
    33961 agaaggcaaa ttgcttcatg gtgttgctgg tgcagcaggt gaacttggtc atatcactgt
    34021 tgactttgac caaccaatct catgtacttg tggtaaaaaa ggctgccttg agacagttgc
    34081 ttcagcaaca gggattgtca acttgactcg tcgctatgcc gatgaatacg aaggcgatgc
    34141 agccttgaaa cgcttgattg ataacggaga agaagtaact gctaagactg tctttgatct
    34201 cgcaaaagaa ggagacgacc ttgctttgat tgtttatcgt aacttctcac gttacttggg
    34261 aatcgcttgt gctaacattg gttcaatcct aaacccatca acaatcgtca tcggtggtgg
    34321 tgtgtcagct gcgggagaat tccttctaca aggtgttcaa aaagtttacg atgaaaatag
    34381 tttcccacaa gtacgcacat ctactaaatt agctcttgca actctaggaa atgacgctgg
    34441 agttatcgga gcagcatcac ttgtattgca ataagataat gagagaggaa aatgctaacc
    34501 tagagttagt aatgttcctc ttttattttt tatgctatac tagttaggac aatgaatgag
    34561 gtgagagtaa atgacaaaag cagatacgat ttttaaagag aatattgaac gcattctaaa
    34621 ggaaggtgtt ttctcagagc aggctcgtcc taagtacaag gatgggactg ttgctaattc
    34681 caagtatgta actggtgcct tttccgagta cgacttatct aaaggagaat ttcctatcac
    34741 aaccttgcgt cccattgcca tcaaatccgc catcaaagaa gttctctgga tttaccaaga
    34801 ccagtcaaat agcttagaag tgcttaatga caagtacaat gttcactact ggaatgactg
    34861 ggaagttgga gacacgggaa ccattggtga gcgctatggt gccgttgtta agaaacacga
    34921 cattatcaat aagcttctca aacagttgga aaccaatcct tggaaccgcc gcaatattat
    34981 ttcgctctgg gattaccaag ctttcgaaga aacagatggg ctgctcccgt gcgcctttca
    35041 gaccatgttt gatgtccgcc gtatagatgg agaaatctat ctggatgcga ccttgaccca
    35101 gcgctcgaat gatatgctgg tggcccacca catcaacgct atgcagtatg tggctttgca
    35161 gatgatgatt gccaaacatt ttggctggaa ggttgggaag ttcttctact tcatcaacaa
    35221 cctccatatc tatgataatc aatttgaaca agctcaggaa ttgctccgtc gggagccgtc
    35281 aaactgccaa ccacgcttgg ttttaaatgt tcctgatggg actaatttct ttgatatcaa
    35341 agcagaagat tttgagttgg tggattatga ccctgttaag ccacagttga agtttgacct
    35401 agctatttaa aagaatagaa aaaagaagtt gagaataatc ccaacttctt ttgtttctta
    35461 acgtgatacg cggcgacgag ctgctttttt acggttttct tcgatgaaag ctgctttttg
    35521 ctcttctggt tcgattactt tctttttaaa tgcgtatact gcacctgcaa cggcagcgac
    35581 agttcctgcg acacctgtta caagaccttt agcgaatcct ttagccatga gtcttcctcc
    35641 tttatattct caatcagcca gcctcctcaa gaggtcacat ttttctgact gacctttttg
    35701 tgttataata atagtaacga aaaaatggga atttttcaag gaaaaaagat gaaaacaaaa
    35761 ataattgtga ttgttggacc gactgctgtt ggaaagacgg ctctagctat tgaagttgca
    35821 aagcgtttta atggcgaagt ggttagtgga gatagccagc aagtctatcg aggacttgat
    35881 attgggacgg ccaaggctag tccagaagag caggcagctg ttcctcatca tttaatcgat
    35941 gttagagaga taaccgagtc ttactcggct tttgattttg tttcagaagc taagatgact
    36001 attgagggta ttcacaaccg tggcaagcta gccattatcg ccggtgggac tggactttat
    36061 atccagagct tgctagaagg ttaccaccta ggtggggaga ctcctcatga ggagatttta
    36121 gcttatcgag ctagtttgga gccatattca gatgaggaat tagcccattt ggtggatcaa
    36181 gcaggccttg agattcccca gtttaatcgt cgtcgtgcta tgcgtgcctt agaaattgcc
    36241 cattttggtc aggatttgga aaatcaagag accttgtatg aaccgctgat tatctgcttg
    36301 gatgatgaac gtagtcaact ttatgagcgt atcaaccacc gagtggattt gatgtttgag
    36361 gctgggcttt tggatgaggc caagtggctg tttgaccatt cccctaatgt acaggctgct
    36421 aaaggaattg gctataagga actctttcct tatttccgtg gagagcagac cttggaggaa
    36481 gctagtgaga gtctcaaaca ggcgacccgt cgttttgcca agcgtcagct gacctggttc
    36541 cgtaatcgca tgcaggtcac cttttatcag attggagaat ctggtgtaca agaccgcatt
    36601 ttaagccaga tagaggagtt tttagatgat tgaaacggag aaaaaagagg agcgagtcct
    36661 gctgattggt gtggaattgc agggtatgga cagttttgac ctctccatgg aagaattggc
    36721 tagtttagcg aaaacggcag gggcagtcgt tgtagatagc tacagacaaa aacgtgaaaa
    36781 atatgattcc aagaccttcg tcggctctgg taagttggaa gagattgcgc ttatggtgga
    36841 tgcagaagaa atcactactg tcatcgtcaa caaccgtctg accccaaggc agaatgtcaa
    36901 tctagaggaa gttctcggtg ttaaggtcat tgaccgtatg cagttgattt tggatatctt
    36961 tgccatgcgg gctcgaagcc atgaagggaa gctccaagtc cacctagccc aactcaaata
    37021 ccttttgcct cgcttggttg gtcaggggat tatgctcagc cgtcaggcag ggggaattgg
    37081 ttcccgtggt cctggtgaaa gccagctgga gctgaaccgt cgtagcgttc gcaatcaaat
    37141 cacggatatt gagcgccagc tcaaggtggt tgagaaaaat cgggcgactg tcagagaaaa
    37201 acgtttggag tctagcactt ttaagattgg tttgattggt tatactaatg ctgggaaatc
    37261 aactatcatg aacatcttga ccagtaagac ccagtatgaa gcagatgagc tctttgcgac
    37321 tctggatgcg acaaccaaga gtattcatct gggaggcaat ctccaagtaa ctttgacaga
    37381 taccgttggc tttatccaag atttgccgac agagttggtg tccagtttca agtcaacctt
    37441 ggaagaaagc aagcatgtgg accttctggt tcatgttatc gatgctagca atccttacca
    37501 cgaggagcat gaaaaaacgg ttctctccat catgaaagac ctggacatgg aagatattcc
    37561 tcacttgacg ctttataata aagcggattt ggtggaggat ttcacgccta cccaaacgcc
    37621 atataccctc atttctgcca agtctgagga cagtcgtgaa aacttgcaag cattattgct
    37681 agataagatt aaggaaattt ttgaagcatt taccctgcga gtgccttttt caaagtccta
    37741 caagattcat gatttagaga gtgttgcaat tctggaagaa cgtgattatc aggaagacgg
    37801 cgaagtgatt acaggctaca tttccgagaa aaataaatgg aggttagaag aattttatga
    37861 ctgatattaa aactttggct ctaaagtatg gaggttatac aagtctggac aaggtctatc
    37921 tggatcagct tctagctggt aaaacagagc aggagcagtt aacacttatt acacctccgc
    37981 caagtgttgt taatgcctac tttgcagaac tctatcagaa aaaaagtcct caggctgcga
    38041 cggattattt tgcagaactc agtcaggaac tgaatcttta caatactgag ccaagtttca
    38101 ccctagaaaa taagcctttt attcggctta atctgtctgg taaatccttt ggtttttgtt
    38161 atgagagtga cggctttggt cggattttct ctgaaaatga ggagaaaatc tcggatgact
    38221 tgctctttga aattgcgcaa attttctccc atcaattagt ctttgaagag tctggtaaga
    38281 tttacatgaa gcctgtcaag gacgaggaag ttgtcagcct agaaagtctc acagctttga
    38341 ctgatttgga aagattggct gatggtcgaa aacgtctcaa aggctacagc caagaggatt
    38401 tattgcaaga agctgctgct ttttctggca aacgctattt ccgatcggaa aaccgcacag
    38461 ccatgttata tattgattaa ttagaaagta tcgaatggat attcaatttt taggaacggg
    38521 ggctggtcag ccctctaaag cccgcaacgt ttcaagtctc gccctgaaac ttttggacga
    38581 gattaacgaa gtttggctct ttgactgtgg agaaggtacg caaaatcgca ttctggaaac
    38641 cacaattcga ccacgtaagg tcagcaaaat ctttattact catctgcatg gagaccacat
    38701 ttttggtttg ccaggtttcc tttctagccg tgcctttcag gccaatgaag agcagacaga
    38761 tttggaaatc tacggacctc aaggaatcaa gtcatttgtc ttaaccagcc ttcgtgtgtc
    38821 aggttctcgt ctgccctacc gcattcattt ccatgagttt gaccaagatt ctctgggtaa
    38881 aattcttgaa accgataaat tcactgtgta tgcagaggag ctggaccaca ctattttctg
    38941 tgttggctat cgtgtcatgc aaaaggatct agaagggacg ctggatgctg aaaaactcaa
    39001 ggctgctggt gttccgttcg gcccgctttt tggtaaaatc aaaaacggcc aggatcttgt
    39061 tttggaagac ggaactgaaa tcaaggcagc agactatatc tcagcgccac gtccaggtaa
    39121 gattatcact attttaggag acactcgaaa aacggatgcc agtgtgcgtc tggctgtcaa
    39181 tgcagatgtc ctagttcatg agtccactta tggcaagggt gatgaaaaaa ttgctcgtaa
    39241 ccatggtcac tcaactaata tgcaagctgc acaagtagcg gtagaagcag gtgccaaacg
    39301 cctcctactc aaccatatca gtgcccgttt cctctcaaaa gatataagca aactcaagaa
    39361 ggacgctgcc acaatttttg aaaatgtcca tgtggtcaaa gacttggaag aagtggaaat
    39421 ctagcagtca cagaaaggat aagtatgcct actattctca ttaccggagc tagcggtggt
    39481 ctagctcaag aaatggtcaa actcctgccc aatgaccaac tcatcttgct tggtagaaat
    39541 aaggaaaaat tagcccaact ctacggaaat tattcccatg cagaattgat tgaaattgat
    39601 attaccgacg attcagccct agaagctctg gtaactgatc tttatctccg ctatggcaag
    39661 attgatgtct tgattaacaa cgctggttac gggatttttg agggatttga ccagattgct
    39721 gataaagata ttcaccagat gtttgaggtc aatacctttg ccctgatgaa tctgtctcgt
    39781 caccttgcgg ctcgtatgaa ggaaagcagc aaagggcata tcatcaacat cgtcagcatg
    39841 gcaggtctaa tagctactgg caagtctagt ctttactcag cgaccaagtt tgcggctatt
    39901 ggtttttcaa atgctctgcg actcgaactt atgccctatg gagtctatgt gacaacagtc
    39961 aatccaggac caatccgtac aggatttttc gaccaagccg acccagatgg aacttatctt
    40021 aaatcggttg accgcttcct gctagaacca gatgcagtgg ctaaaaagat tgtcaagatt
    40081 ataggaaaaa acaaacgaga actcaatctc ccgattttgt tgaacctagc tcataagttt
    40141 tataccctct ttcctaagct agctgataag ttggcagggg aaacttttaa ttataagtaa
    40201 aaagaaccaa tgtgcaggtt gttgctagcc tacatattgg ttcttatcat ttctcaacta
    40261 tcaaactgaa cttcttctag gaggtagtct ataaagcgtt cgcccatctt agataggctg
    40321 gttttctcat gctggatata gaccagctca atcgggtcat caatatccag tgggatagaa
    40381 acgatattgt ctccgtttag gttgctgttc aaaatccctg tcgcaatggt ataaccatcc
    40441 aaaccaatca agagattaaa gagggtggca cggtcactga ccacaatgga tttcttgtgg
    40501 tgttcttgag aaagaatctc ttctgaaaag tagaaggagt tgtgcgtccc ttggtcatag
    40561 ctgaggtaag ggaaattctc caaatcagac agtttcacct tgtctttctt tgccagaggg
    40621 ttggtcttgc tgacaaagat atgtggttgc gctgtgaaga gatggtgggc tagcaggtga
    40681 ttgtcatcca gcatcttggt taaaacatca cggttgtaac tgtttaagaa gaggaccccg
    40741 acctcactgc ggaagttctt gacgtcgtcg ataatctccc aagtccgagt ttcacgaagg
    40801 aagagttcgt atttctccat atcgcttttc ttgagcaaag agacaaaggc attgaccaca
    40861 aaggcatagt gttgagacga aacgctaaat agttcgcggt gggcgacagg atttttatag
    40921 cgttcctcca gaagctgggt ctgctcgaca acctgacggg cataggagag aaactccatc
    40981 ccatcacggg tcaaggtgat tcccttggga ttgcggataa agatctcaat gcccatttca
    41041 ttttccaaat ctcgcactgc attggagaga cttggctgag tgataaagag ttgcttggct
    41101 gcctcattca tggagccagt ttcgacgatt ttgataatat aatgtaattg ttgaattctc
    41161 atgtttttat tgtaccatac ttgccttaga atcgctaaag aagatgggaa aataaggagc
    41221 attgtgtgag tctcttcttg aaaaaaacac aaaattttgc tagaatgagt cttatgaaaa
    41281 cattctatga tgtgcagcaa ttcctcaaac gatttggtat tattgtttac atgggaaaac
    41341 gcttatatga tattgaactg atgaagttgg aactatctcg gatttacgat gcagggttga
    41401 tggataaatt agactatcta gaggcagaag cagttcttcg cagagagcac aaggtagaat
    41461 tggattatat tgagaaaaat ggagaaaaga actaatggtt acttggattt tgtgggcact
    41521 tatactagca atgttggcgt ggatgggctt taactatctt cgtattcgcc gtgcggctaa
    41581 aattgtggac aatgaggagt ttgaagcctt gattcgtacg ggtcaattga ttgatttgcg
    41641 cgacccagca gaattccaca gaaaacatat ccttggtgca cgcaatattc cttcaagtca
    41701 gttgaaaact agtcttgcag cccttcgtaa agataaacct gtccttctct acgaaaacca
    41761 acgtgcgcaa cgagttacaa atgcagctct ttacttgaaa aaacaaggtt tttctgagat
    41821 ttatatcctt tcttatggct tggattcttg gaaagggaaa gtgaagacta gctaacctta
    41881 tttccaaatt agttgaatga taggatgaag ctattgtttt atagtataat taatttgtat
    41941 aaaatttaag gagttcttag aacatgaaag aaaagaagaa aatccacttt ttgtgggtat
    42001 cttgtcgatt attcttggtt tgttatttcc aattgtaggc ctgattttgg gtatcatagg
    42061 attggtcttg gctatttcat accaaaaaga gt
//
LOCUS       DAVKQB010000013        41001 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKQB010000013 DAVKQB010000000
VERSION     DAVKQB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41001
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.013"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..471)
                     /locus_tag="VW528_000681"
     CDS             complement(<1..471)
                     /locus_tag="VW528_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEU9911535.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKP"
     gene            719..1144
                     /locus_tag="VW528_000682"
     CDS             719..1144
                     /locus_tag="VW528_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9911536.1"
                     /translation="MELSIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDVLLLFTYLHMAAAPEL
                     ADAMLAAKITGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            1145..1558
                     /locus_tag="VW528_000683"
     CDS             1145..1558
                     /locus_tag="VW528_000683"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9911537.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGGGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            1504..1830
                     /locus_tag="VW528_000684"
     CDS             1504..1830
                     /locus_tag="VW528_000684"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911538.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(2197..2457)
                     /locus_tag="VW528_000685"
                     /pseudo
     CDS             complement(2197..2457)
                     /locus_tag="VW528_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(2460..2960)
                     /locus_tag="VW528_000686"
     CDS             complement(2460..2960)
                     /locus_tag="VW528_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9911539.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVIDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            2996..3175
                     /locus_tag="VW528_000687"
     CDS             2996..3175
                     /locus_tag="VW528_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911540.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            complement(3153..4121)
                     /locus_tag="VW528_000688"
     CDS             complement(3153..4121)
                     /locus_tag="VW528_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9911541.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(4207..4422)
                     /locus_tag="VW528_000689"
     CDS             complement(4207..4422)
                     /locus_tag="VW528_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9911542.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            complement(4431..5285)
                     /gene="cvfB"
                     /locus_tag="VW528_000690"
     CDS             complement(4431..5285)
                     /gene="cvfB"
                     /locus_tag="VW528_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9911543.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGCVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(5345..5902)
                     /gene="frr"
                     /locus_tag="VW528_000691"
     CDS             complement(5345..5902)
                     /gene="frr"
                     /locus_tag="VW528_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9911544.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(5911..6648)
                     /locus_tag="VW528_000692"
     CDS             complement(5911..6648)
                     /locus_tag="VW528_000692"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9911545.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(6732..8066)
                     /gene="trmFO"
                     /locus_tag="VW528_000693"
     CDS             complement(6732..8066)
                     /gene="trmFO"
                     /locus_tag="VW528_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9911546.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(8361..8615)
                     /locus_tag="VW528_000694"
     CDS             complement(8361..8615)
                     /locus_tag="VW528_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9911547.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            8610..9414
                     /locus_tag="VW528_000695"
     CDS             join(8610..8978,8980..9414)
                     /locus_tag="VW528_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9911548.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVKINRPKKQLANHSGKKKFHAMKAQAIVTSQGRIVSLDIAVNY
                     CRDMKLFKMSRRNIGQAGKILSDSGYQGLMKIYPQVQTPRKSSKLKPLTAEDKACNHA
                     LSKERSKIENIFAKVKTFKMFSTIYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(9403..10049)
                     /locus_tag="VW528_000696"
                     /pseudo
     CDS             complement(9403..10049)
                     /locus_tag="VW528_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(10158..10676)
                     /locus_tag="VW528_000697"
     CDS             complement(10158..10676)
                     /locus_tag="VW528_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9911549.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(10903..11772)
                     /gene="rsmI"
                     /locus_tag="VW528_000698"
     CDS             complement(10903..11772)
                     /gene="rsmI"
                     /locus_tag="VW528_000698"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9911550.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(11775..12092)
                     /gene="yabA"
                     /locus_tag="VW528_000699"
     CDS             complement(11775..12092)
                     /gene="yabA"
                     /locus_tag="VW528_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9911551.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(12132..13022)
                     /locus_tag="VW528_000700"
     CDS             complement(12132..13022)
                     /locus_tag="VW528_000700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9911552.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(13019..13657)
                     /locus_tag="VW528_000701"
     CDS             complement(13019..13657)
                     /locus_tag="VW528_000701"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9911553.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLELHKKVRQGYLSLLEKEGNRIVKIDASLPLEQVVETTKAVLFDGMDLAK"
     gene            complement(13877..14674)
                     /gene="proC"
                     /locus_tag="VW528_000702"
     CDS             complement(13877..14674)
                     /gene="proC"
                     /locus_tag="VW528_000702"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9911554.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFYQLLAKAGLLVELGES
                     LIDAATGLAGCGPAFVYLFIEALADAGVQTGLPREIALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMDAVHQAYKRTQELGK"
     gene            complement(14678..15940)
                     /locus_tag="VW528_000703"
     CDS             complement(14678..15940)
                     /locus_tag="VW528_000703"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9911555.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAIGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(15950..17080)
                     /locus_tag="VW528_000704"
     CDS             complement(15950..17080)
                     /locus_tag="VW528_000704"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9911556.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(17160..19043)
                     /gene="cbpE"
                     /locus_tag="VW528_000705"
                     /gene_synonym="pce"
     CDS             complement(17160..19043)
                     /gene="cbpE"
                     /locus_tag="VW528_000705"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9911557.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(19046..19933)
                     /locus_tag="VW528_000706"
     CDS             complement(19046..19933)
                     /locus_tag="VW528_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9911558.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(19923..20384)
                     /locus_tag="VW528_000707"
     CDS             complement(19923..20384)
                     /locus_tag="VW528_000707"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9911559.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(20381..21289)
                     /locus_tag="VW528_000708"
     CDS             complement(20381..21289)
                     /locus_tag="VW528_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9911560.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            21282..21479
                     /locus_tag="VW528_000709"
                     /pseudo
     CDS             21282..21479
                     /locus_tag="VW528_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(21531..22202)
                     /locus_tag="VW528_000710"
     CDS             complement(21531..22202)
                     /locus_tag="VW528_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9911561.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(22256..22477)
                     /locus_tag="VW528_000711"
     CDS             complement(22256..22477)
                     /locus_tag="VW528_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU9911562.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            22749..23558
                     /locus_tag="VW528_000712"
     CDS             22749..23558
                     /locus_tag="VW528_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9911563.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(23739..24614)
                     /gene="aguB"
                     /locus_tag="VW528_000713"
     CDS             complement(23739..24614)
                     /gene="aguB"
                     /locus_tag="VW528_000713"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9911564.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(24624..25709)
                     /gene="aguA"
                     /locus_tag="VW528_000714"
     CDS             complement(24624..25709)
                     /gene="aguA"
                     /locus_tag="VW528_000714"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9911565.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(25706..26833)
                     /gene="nspC"
                     /locus_tag="VW528_000715"
     CDS             complement(25706..26833)
                     /gene="nspC"
                     /locus_tag="VW528_000715"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9911566.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLEIMDHIVF
                     NSERQLRKHGPRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(26833..28092)
                     /locus_tag="VW528_000716"
     CDS             complement(26833..28092)
                     /locus_tag="VW528_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9911567.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(28089..28949)
                     /gene="speE"
                     /locus_tag="VW528_000717"
     CDS             complement(28089..28949)
                     /gene="speE"
                     /locus_tag="VW528_000717"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9911568.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            29204..29919
                     /locus_tag="VW528_000718"
                     /pseudo
     CDS             29204..29919
                     /locus_tag="VW528_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(29885..31360)
                     /locus_tag="VW528_000719"
     CDS             complement(29885..31360)
                     /locus_tag="VW528_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9911569.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            32067..33062
                     /locus_tag="VW528_000720"
     CDS             32067..33062
                     /locus_tag="VW528_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9911570.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVRKSSVTKELKEKILHYHNQ
                     KFSPEMMVMVKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKSAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSADVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGIKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSVRNKSRN"
     gene            33088..33732
                     /locus_tag="VW528_000721"
     CDS             33088..33732
                     /locus_tag="VW528_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9911571.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATSNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(33774..35762)
                     /locus_tag="VW528_000722"
     CDS             complement(33774..35762)
                     /locus_tag="VW528_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9911572.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(35764..36522)
                     /locus_tag="VW528_000723"
     CDS             complement(35764..36522)
                     /locus_tag="VW528_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911573.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            36950..37141
                     /locus_tag="VW528_000724"
     CDS             36950..37141
                     /locus_tag="VW528_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911574.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(37495..37767)
                     /locus_tag="VW528_000725"
     CDS             complement(37495..37767)
                     /locus_tag="VW528_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9911575.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(37825..38274)
                     /locus_tag="VW528_000726"
     CDS             complement(37825..38274)
                     /locus_tag="VW528_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9911576.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            complement(38261..40390)
                     /locus_tag="VW528_000727"
     CDS             complement(38261..40390)
                     /locus_tag="VW528_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9911577.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            complement(40492..>41001)
                     /locus_tag="VW528_000728"
     CDS             complement(40492..>41001)
                     /locus_tag="VW528_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HEU9911578.1"
                     /translation="GGLIYKQLGFQYRTPFVGLFILPDDYYKLTKHFRYYMEQELVFE
                     EELISPSWTHEVYPLGHLGDIDIHFLHYSSIEEARTKWEKRKKRINWNNLYFKFDNKD
                     SASEEILNKMDNLPYSNKLILVNRPYKNLKSQCVIPGQEHLPELAIMSDPLNTFDIMA
                     WLKKGGNAL"
BASE COUNT        11997 a         9168 c         7570 g        12266 t
ORIGIN      
        1 cggcttaaag ttcggactag cttgcttctt gacacctttc ctttttctgg gataaagcat
       61 gtcggccttc gtcaatccta aatgaccatt atgaaaccag tagtagatgg ttgaaatacc
      121 aactttcact tgcttcttgt taaccatcat ttcaggcgaa aatttttgct tatgatagtg
      181 taagatcttc tctctgattt cctttgttaa aattaacttt ttcaccgacc gttttcgatt
      241 gaattggtaa acagtttgtg cgtaatcggc agaatagacc tttttgtata gcccttttct
      301 cacttgttgt aaagttgtac ctcttttgac ttcattatga atcgtttgag gcgcctttcc
      361 taacaagcct gcaatttcac gattggactt attttcattc ttccaccgct caatcaagcg
      421 acggttatct attgtcaaat gtttcccttt tggggtataa tgttcttgca tctctgtgcc
      481 tttccttgtg tttgtggtga acaacaagta tagcacagag gtgttttctt ataccttaaa
      541 tcacatttca tttgacaaca ggaacccttg aacctgtttt ttcagctaaa ccaaggctat
      601 gattagcctt ggccacctag cctaacagtt tcaaacctgt tatcaaatga aatcatcaac
      661 ttactcaaac atagtgaaac atctaattct tgactaaggt aaggtggcta acttcattat
      721 agaactttca atcccaaaag aaattaaaaa taacgaaaac cgtgtcgccc tcacacctgc
      781 aggtgttcat agcttagtta gtcgtggtca tcgtgtcctt atcgaaacaa atgctggtct
      841 cggttctggc tttactgatg ctgactatca aaagcaagga gctgagattg tcgctactgc
      901 tggtgaagct tgggcagcag agttggttgt gaaagtaaaa gaacctttaa gttctgaata
      961 cggttacttg cgcgacgttc ttcttctctt cacctacttg cacatggccg ctgctccaga
     1021 attagcagat gctatgttag cagcaaaaat aacaggaatt gcctatgaaa ctgttcgtga
     1081 caatcaagga caactaccgc tcctcgttcc tatgagtgag gttgcaggtc gtatggctgt
     1141 ttaaatcgga gctcacttcc ttactaagca agctggtggc tctggtgttc tacctggtgg
     1201 tgtaccaggt gttccaaaag gaaaagtaac tatcatcggt ggtggtgtcg tcggtacaca
     1261 tgctgcccgc atcgcccttg gtcttggtgc tcaagtgact attttagata ttagtgccaa
     1321 gcgtctctca gttctagaag aagtctttgg aagtcaaatt caaactctta tatctaattc
     1381 attcaacatt gaagcaagta tgagagatgc tgatgtggtg attggagcca ttctcatccc
     1441 tggtgcaaaa gcaccggaat tggtgacaga tgagatggtc aaacaaatgc gtccaggctc
     1501 tgtatcgttg acgttgctgt tgaccaaggt ggcgttatcg aaacagctga ccgtgtgaca
     1561 acgcacgatg aacccgtcta tgaaaaacac ggtgttctcc actatgccgt tgccaatatc
     1621 cctggtgcgg ttgctcgcac ttcaaccatc gccctaacca atgtcactct tccttatatc
     1681 gaagctttgg ctggcaaagg attcgcacaa gcaatctctg aagatgaagg cttgcgtcaa
     1741 ggtgtgacca cttatcaagg ttacttgact agcctaccag ttgctcaagg acttaatcgt
     1801 gactacactg atatcaatga tttagtataa attttgaaaa gaaaagggtg acttcgcaag
     1861 tcctacccac tgtgctttgt gtcatcagct attacatcgt tggtttattg tactgtatac
     1921 tttgcaactg gagactttcg ttccagactc aattgtgcat aagaaactct aaattgaata
     1981 aagtcaacag agtttcgccc aaccacttcg tcttgtaatg acaattatat aaaccaaagg
     2041 aagctgagct ctttagtccc agcttctttt tatatataaa attttacccg tgcaaagaca
     2101 gggccttagc agacttcttt tttacttcgt tcacccttgc tttttctttt taggtttggg
     2161 cgttggcagt tggttataca tagctaaaat caggtcttat agaaacatct tattatcaag
     2221 ttcttccact caaatcattt ctttggcacc tttgtatgga aactcaaaaa aagattggtc
     2281 aatcttatct aagactgctt gcacgggttt aactaaaaag cgatcatcat aaatgccgcc
     2341 aataatcttg ccgcggaagt aaagaatata ctcccccatc atggaacggt aagtcacatc
     2401 atctaatcct gataattgtt ccaaaacaaa ttccaaatag ttcttacttg atgccatttc
     2461 taatcttcta ggctctgttc agcgataaca accgtataga gttcttgctt aacctcgcat
     2521 ccaattgatt taaagccctg cttttcccaa aaatgctgag attgcggatt tcccttaaca
     2581 taagccaaac gtgcctttcg aaagttctta gcaaaataag ctagtgcttc tgtcacaata
     2641 tgactaccaa tccctttcct ctgataggct tgatcaacca taaacaaacc aataaaaaca
     2701 gtctcctcat caggatgtgc atagacaaaa tctataacag ccacaaggtc agatccattc
     2761 caaaatccaa caaaaaactt atcagcctta gctttacctt caggtagaca aagcatgtcc
     2821 tcttttacag ttgcaaaatt tggctctggt ggacaatgct gaaaatacag aggattactt
     2881 tcatataaag ataaaatact tggaatatcc ttttcagtta gtatcctaca actgtaatac
     2941 ttagatagtt ggtcaatcat cttttcaaat tcctactttc ttgtgccctg tgattatgac
     3001 acaggaagat gcactgatcg tcatcagcca cataaaagtt ctttccatcg tgcctaatcg
     3061 ttgtctcaaa cctttggata aaacctttag cctatacaac tggattttcc tctctcaaaa
     3121 gtatattctt ttgcaggcaa acttcctcaa aatcagtcgt gtgcaacttc agtagaatat
     3181 tcataggctc ggataatctg agcgacaact ggatggcgaa ccacatcctt ggctgaaaaa
     3241 tgaacaaagt caatctgatg gatgttcttg agtttctctt gagcatcaat caaaccggac
     3301 ttgacattac gtggcaggtc aatctgacta atatctccat tgacaatcat cttagaatga
     3361 aaacctaaac gagtcaagaa catcttcatc tgcatgatgg tcgtattttg cgcctcatcg
     3421 agaatgacaa aggcatcatc caaggtccgt ccacgcatat aggcaagggg cgcaatctcg
     3481 ataatttcac gctccatgag tcgagttgtt tggtctttcc ccagaatctg atacaaggca
     3541 tcgtaaacag gacgaaggta aggatccacc ttctccttaa gatcacccgg aagaaatcca
     3601 agactctctc ccgcttccac cgctggacga gttaggataa ttcgcttgac ttgcccacgt
     3661 ttaagggcag tcactgccaa ggtcactgca aggaaggtct tccctgtacc tgctggccca
     3721 attccaaagg tcacatcatg ctgtttgaca ctgtccacat aaagcttttg ccctagggtt
     3781 ttgacacgga taggtttccc agtattatct ttgataattt cttcttcgta aagggcgaca
     3841 aacttgtcaa tttcatcatt tttgaccatg ctaatcgcag tgactacatc tggcgtacca
     3901 acggtcatcc cacgatttac caagaccatc aaagcctgaa taacttgacg ggcttcctca
     3961 caggcagact cttctcccaa aacctggaca atctccgtac gagcatgaat cacaacatca
     4021 agctcttctt ccatcaaacg aagatggcgt tcattggaac caaaaagatg aaacaggtca
     4081 tctggatgac tcagttgaat gtctattgaa tgttccttca aataaagaac ctctctaatc
     4141 cttttatttc tttttattat agcagaggga tggagaaatt actagcccaa acccaatgaa
     4201 taaatgctag tgttctagat attcctgcca aatgctgtca aaatctccta ggttaaaaga
     4261 gaaactggca tgctcctcca aaaagcgact gacctcatca aaatcatctg tatgttttgg
     4321 aaaagctgcc tcttcaaaag cgaggtctgc caaaattgct ttgggactgt tacttttagg
     4381 attgcgctcg gtcatgagcc aagtgtaaaa tgattttctc ataagcctcc ctaaatcaac
     4441 tctgtcccaa actggtcctg cttgatttta ccagccttca taagaccacc taaagctttc
     4501 ttgaactgac ctttagaaat gccaaaggtt gccttgatgt cgtctggaga tgacttatca
     4561 tttaaggtca tgaaaccgcc attactttcc aaataagtca aaatcatctg agcatcgttt
     4621 tccaacattt caaaggagcg tggtttgagg gagaggttca gagtgcggtc cacttcacgg
     4681 aaaccaataa cgcgcgcatc taatacttgc cccaaacgtg gctctgcgta acgctcgcta
     4741 ggatgaataa aaccaagcat attattttct ggtaggtaaa caaaagttcc tgacagcttg
     4801 agacggtaaa caatggctgg ccagttttgg ttctgcatgt tgttgtaggc aggacgagca
     4861 agacgttgga agtcttcttg ataagccaag aggccccaga tacggtcttt cttatccact
     4921 tcaagacgga tgtagagttg gtcgcccttc ttaggccaga gttccttgag ctcagggaga
     4981 atatcgagtg acacaacgat ttccttgtca ggaaggcctg tatctacaaa gacacccaag
     5041 tccttacgaa cctctgtgac acatccccaa ccaaattggt cctgagtggc agtcacttct
     5101 aaggttgtca ggcggagttt ttgcttcata tccgtgtatg caaaaccttt gaccgtatcc
     5161 cctactgtat gttggccctc ttccttagca agagcatagg tttgaccatc cttttgcaca
     5221 aagtaaaaac ggtcattttc atcgatgatc agtccaacga taaaacttgc aagatttgta
     5281 ttcatatttc cttctttcga ataaaactca gccagcaatg ccaactgagt ttttctgttt
     5341 atttttagac ttccaaaagt tctttctctt tgttagcagt catgtcgtcg atgtgtttaa
     5401 cagcatcgtc tgttactttt tgaatatctt tttcaagagt cttcaattcg tcttcagtga
     5461 tttcttttgc tttttcttgt ttcttagctt cgtccatagc atcgcgacgg atattgcgga
     5521 cagccacttt agcattttcg ccgaccttct tcacttcttt agcaaggtca cgacgagttt
     5581 cttctgtaag agctgggata accaagcgaa tcacagaacc gtcattagcc ggtgtgatac
     5641 caagatcaga agcgttcaag gcacgttcga tgtctttcaa tgaagacttg tcaaatggtg
     5701 ttaccaacaa aacacgcgct tctggaatcg taattgaagc gatttggtta agaggagttt
     5761 cgactccata gtattctaca tgtacacggt caagcaagct tgcattggca cgaccagcac
     5821 ggataccacc aaattcacga gcaagtgatt ggtgagattg ggtcattctc tctttagctt
     5881 tttcaataat tacgttagcc atattctttc ttattccttt tcttcgatat tatttgaaac
     5941 tgttgttccg atattttcac caaatacgac acgtttgatg ttgcctgatt ggttcatgtt
     6001 gaagacaacc aagtcaatgt cgttgtccat tgagagggtt gaggctgttg agtccatgat
     6061 acgaagacct ttgttgataa catcacggtg ggtcaattct tcaaacttaa cggctgtctt
     6121 gtccttctta ggatcggcat tgtacacacc atcgacgcca tttttagcca tgaggatggc
     6181 atctgcttcg atttcagctg cacgaagggc cgctgttgta tctgtcgaga agtatggtga
     6241 accaattcca gcaccaaaga taacgatacg gcctttttca aggtgacgaa gggcacgtcc
     6301 acggacataa ggctctgcca cttgttgcat agcaatagct gtttgtacac gcgtatcaac
     6361 cccaacttgt tgcaatgaat ctgccatcac aagagcattc ataacagtcc caagcattcc
     6421 agtgtaatct gcctgaacac ggtccatacc tgcttctgct gcaggttctc cacgccaaag
     6481 atttcctcca ccaataacaa gggcaatttc gatacctaag ctatgaactt cttgaatctc
     6541 ttttgcgatt gtttgaactg tttggatatc aatccctacg ccacgttcac cggcaagggc
     6601 ttcacctgat aacttgatta aaatacgttt atacttggga ttcgccattt tcactctcct
     6661 tctttcatcc tacctatttt atcacaattt ctaagatttt tatagtatca tgagcaattc
     6721 tttcaaaaaa attagacagt caaaaattcc tctaagtcgg caagggcacg ctctgcaatt
     6781 ttttcataac gagccttctt atcacggata cgctcgcctt ccaactcctt gatgatccca
     6841 aaattgacat tcattggttg gaaatgtttg ctgtcggcat gggtaatgta atgagctaag
     6901 cttccaatcg ctgtcgtctc ggggaaaata acctcgcttt cttccttgaa gagacgagct
     6961 gcgttaattc ccgcaactaa gcccgaagcc gccgactcaa catagccttc cacccccgtc
     7021 atctgaccag caaagaagag atttggttgt ttcttggaac ggtaagtctg ctcaagaaga
     7081 tttggtgaat ccatgtaaga attgcgatgc atgacaccat aacggacaaa ctccgcattt
     7141 tcaagacccg gaatcatttg gaagacacgc ttttgttctc cccatttgag gtgggtctgg
     7201 aaaccaacaa tattgtagag gctaccagct gcattatcct gacgaagttg cacaaccgca
     7261 taaggtgttt taaattctcc atcacgaggt cctgtgtagt cgtccggata ctcaagaccg
     7321 actggcttca tagggccata aagcatagtt ttaatgccac gtttggccat aacttcgatt
     7381 ggcatacatc cttcaaagta cttttctttt tcaaaagaat tgagcggtgc ttcttccgca
     7441 ttgaccaagg cttcatggaa atccataaac tcttgcttgg tcattggagc attgagatag
     7501 gctgcttctc ccttatcata acgagatttg agatagacct tactcatatc gatagtgttg
     7561 acatcgataa taggcgctgc tgcatcgtag aaatagaagc catcgccatt attaagggca
     7621 tgaatctttt cagccagggc atcgctagtc aagggaccag tcgccacaac tgtaataaca
     7681 tctgttggca attctgtaat ttcatcacgc accacttcaa tcaaggggtg gttgaccact
     7741 ttttcggtca ccatttgaga gaaaccatca cgatccaccg caagggctcc accggcagga
     7801 acacgtgtag cctcagcaga ttccaagata acagaaccca agcgacgcat ttcttccttg
     7861 agaagaccaa ctgcatttgt caaagcatcc ccacgcaaag aattggaaca aaccaactca
     7921 gcaaaattgt ctgttttgtg ctgaggagtc gacttgacac cacgcatttc ataaagttta
     7981 actggaatac cacgctctgc gatttggtag gctgcttcag aacctgccaa accagcaccg
     8041 ataacattga tataagattg agacacgaca ctaatacctc tttgggagtg tgaagttaag
     8101 attcacattg aaaaagccaa tcagacttac gagatttcga gtttcttggc tcaggctgaa
     8161 aaagtccccc agactttcct cgctctcgaa tttcttggct caagctgaaa aagtcccccg
     8221 gactttttca ctcccacaaa tctttctatt tttttcttct actagtataa caaaaaaagg
     8281 gaagaaggca aacttccctg tttagtcatt ttcttgatgt aaagagatag agagtattcc
     8341 agttaagaat caattatatg ctactctata aaatcttttc cacataacgg atcgataggg
     8401 actgttattc tatctcttgc tataaccgta ttatctaaaa cagcataaca ttcaacatag
     8461 tgatctcctt taaactgtga atcttcgtga tattttattt tacctgaaaa aatagcacca
     8521 cgctcacaat ttcttctaat agcctcagct tcaatatttc ttactttcca atagacttcc
     8581 tgcgaaacaa aatatggtat agtagttcta tgaatgatga agcaagtaaa caactaactg
     8641 atgcacgatt taagcgtctt gttggtgttc agcgcacgac ttttgaagag atattagctg
     8701 tattaaaaac agcttatcaa cttaaacacg caaaaggtgg acgaaaacct aaattaagcc
     8761 tagaagacct tcttatggcc actcttcaat atgtgcgaga atatcgaact tatgaacaaa
     8821 ttgcggctga ttttggtatt cacgaaagca acttaatccg tcggagccaa tgggttgaag
     8881 taactcttgt tcaaagtggt gttacgattt caagaactcc tctcagttct gaggacacgg
     8941 taatgattga tgcgacggaa gtaaaaatca atcgccctaa aaaaacaatt agcgaatcat
     9001 tctggtaaaa agaaatttca cgctatgaag gctcaagcga ttgtcacaag tcaagggaga
     9061 attgtttctt tggatatcgc tgtgaactat tgtcgtgata tgaagttgtt caaaatgagt
     9121 cgcagaaata tcggacaagc tggtaaaatc ttgtctgaca gtggttatca agggctcatg
     9181 aagatatatc ctcaagtaca aactccacgt aaatccagca aactcaagcc gctaacagct
     9241 gaagataaag cctgtaacca tgcgctatcc aaggagagaa gcaagattga gaacatcttt
     9301 gccaaagtaa aaacgtttaa aatgttttca acaatctatc gaaatcatcg taaacgcttc
     9361 ggattacgaa tgaatttgat tgctggcatt atcaatcatg aactaggatt ctagttttgc
     9421 aggaagtcta atgtaattct caagctgtga gttcattatg atttgtaaat ccgatatatc
     9481 ttcagcagct aaaaacacat tttgaaatgt tccaagtccg ttttcaccaa aactatattc
     9541 tctcttacta tacttactct tattattctc tatatagtta gagtcaaggt tcttaacctc
     9601 ttgaagttca ttttctttac ttctgaggtt aaaatctttt acttctgaag ttaagttttt
     9661 tgacttctgt aagtcatttt cttttacctg aaatatactc ataaagtctt taacataaat
     9721 gatgttcggc ttaccaagtc caagccttac tctttcgatc agtcctattc cttttttaca
     9781 tcaagctcat ctaatgtttt tatggcagtt ggctttgaga tatttcttct tttcataatt
     9841 tcttcgacag taaaatagat aaatactctg ccttccttgt ctatccagtt attcttaaat
     9901 gacattcctg tctgtttaag aagtatggaa taaaggataa ttgcttctgc tgataagccc
     9961 cgaaattctt ctctatcaac taatatctcc ggtactttta agaagttaaa tctttccgct
    10021 tctctgttat aaaaatattc aaagtccatg caagatcacc tcccttctta aaaatttgca
    10081 aagaaaaaga cgatagtttt tcttctatcg cctttggtaa ccatttttat gaaatttttt
    10141 aaagttaaaa atataattta taatactatc ttcttccatt ttctgatttt agttctgaaa
    10201 gttccaaaag gtgcaaccgt atttacatga ataaacttat acacttccca agtcgctgtt
    10261 ttagtcgctt catcagccca ctttctcata tgcggttcaa ataattcttc ctcactcaac
    10321 gaatcaatca ttgcagagat agaattaata ttttcattta attttgcttt caactcttgc
    10381 agagataaat gagcgtaggt atctgtgaac cactgatata attcaccaag ttgattccat
    10441 ttaaatttat ccgatggtgt ttttacttga agcccctttc tttcatcttc ttcccattta
    10501 agaaccaagt tggtccaacc aacctgataa gcaaggtttt ctgctggagt tctgtcaact
    10561 tcatcagctc tcttatcttt taaattttct ggaatattat caaattctaa aatatatttc
    10621 tcaaatgttt tctctatctc agcttttagt tcttctttat tttcgtatgt tcgcaaaaaa
    10681 tcaccttctt tctgatttat caatcctttg atttaaaatg accttatttt tctatttata
    10741 ccttctaaga acgctataac tgctgctcca accatcggta ttccgtatgg gcttaataaa
    10801 aaacttaata ccaaagcttc tataccaagt gatacatctt tatattatca tcactaatta
    10861 agttaaacag aaacagacag aattattaca tcctgtctcc ctttattgtt tttcttccca
    10921 gtcgtggtag gcagcgtaga gctgactttt attccactgg taaatcttag cgacttcctt
    10981 gatagcttgg tttttcttca caccttgctg gatgcgggtt tgaatttcta cgaacaagtc
    11041 ttcctcgtcc ttttcctcca caccctgact ggcaccctca acaatgagaa gacattcgcc
    11101 cttgagtggc gtttcagcaa tgctttctaa taactcagag atagtacctc gttggtattc
    11161 ttcatagatt ttggtcaatt ccctgaccaa gacaacggag cggtcaccgt agacttctaa
    11221 catattttcc aacgtgtctg ctacacgatg aggtgattca taaaaaatct gtgtttcagg
    11281 ataatctttt ttcaagccaa aaaattgctt ctgctgacct gattttctcg gtaaaaaacc
    11341 gtaaaagata tgtggctgtg gcgctaaacc actggcaatc aaggcagaaa ttcctgcaga
    11401 ggcacctgga actgtcacaa ctgcaatttc ttcctcaata gctgccttaa ctaaatcatg
    11461 accagggtct gaaatgctag gcaaaccggc atcagagacc tgagcaatac tttgccctgc
    11521 tttcaagaaa ccaatcaaat caggaatttt ttccttggca ttgtgctcat gaaaactgat
    11581 ctgcttggtg gaaatgtcaa aatgcttgag caaaagccct gtattgcgcg tatcctcagc
    11641 agcaatccag tccacttctt tcaaggtctg gatagcacga aaagtcatat catctagatt
    11701 gccaatcggc gttgccacta gatacagctt gccataggga gactgcccct taaaactttt
    11761 ttgaatttgc atgcttactc cctgtataac aactcatcac agaacataca ttcttcgtcc
    11821 tgctctcgac gttgtccata aaaatcatta catacgtgaa atccatcacg gtaaatacga
    11881 cggacacttt cgcgaacatg cttggccttg acgggagcgt ctgcttccac ctcacccaag
    11941 cgttctcgca acttactatt ttccaagcga agagctgtat tttcttctac caagctcttg
    12001 agatttttct tgatggcttc cacatcggct aaggttacca ataattgttg ggaaaaatca
    12061 tccagcgcgt caaataattc ttttttgtcc ataaaacagc cctttccttt ctttatcatt
    12121 catttttgag tttatatttc tttcaagacc agatattcca tggcattttg aaagctgaca
    12181 ttagcttgcc acatttttct agcttctagt aaatcttgta gaatcactct tactcttacc
    12241 tgcaagaggt cctgcccaca gagaacttca agaatccgta aaacctgatc ctgtttttcc
    12301 ttatcatctg ccaagttggc taatttggca acctgtagat aactttcttt tttcttagct
    12361 actaaccaag tcagcaggcg ttcactttca tcgaccaagg tccaaaaact tgcctgatta
    12421 gccaactttt ctgcttcagc tcgcgattga ctaaacttag ctaaaagagt cgcttttttc
    12481 ttaacaagtc ccatttgttc taagagtaag ataagttttt cttcttgctt tttaaagtgg
    12541 aagatctgag tccgacttcg gattgtcggt aacatctttt cctcatcgct agtcaagaag
    12601 aaaatataaa cttcactctg gggttcttcg atgaccttga gcagagaatt ggctgcgttg
    12661 ggatgcattt tatccgcttg ctcgataata aagacctgtt gctggctttc aatccctgct
    12721 tgagaaaact gtcccaccaa ttcccgaatg cgttctgtct tgatgacctg attgactggc
    12781 ttaatcaagg tgacatctgg aaactcttcc tgttcaatca gcttgcaact tcggcatttc
    12841 tcacatggta agacgccaac tttatccgta caaaagaggc tcttagctaa aaattgcgcc
    12901 atttccaagc ttccaaagaa acctgaaaag agataggcgt gattgagctg gtcttgttct
    12961 aagatacgga caaaacggtc aaactgagct ggttgccaag cctttagttg atcttgtttc
    13021 atttggccaa gtccattccg tcaaacaaga cagccttggt agtttccaca acttgctcca
    13081 aagggagact agcatcaatc ttgacaatgc gatttccttc tttttccagg agagaaaggt
    13141 agccttgacg gactttcttg tgcaattcca aaccttccaa gtccagacga ttgacctcgc
    13201 ggtcactatt cgcagcaatg cgagccagcc cttcttccac ctcgatgtca aaatagagtg
    13261 tcaaatcggg tttgaggcca tctgtcgcaa actgattgag ccagtcaatg gcttcaatat
    13321 ctaagccacg accaaatccc tgataggcaa cagaactatc gataaaacga tccatgatga
    13381 ccaacttgcc agcttcaagg gctggaagaa ctttttccac caaatgctgt ctgcgactgg
    13441 caatatagag aagtagctct gttttagcat ccatctgagt atgacttgga tccaaaatca
    13501 cttcccgaat cttctcccca atcaagactc cgccaggttc acgggtcgtc agcaccttta
    13561 ctcctttttc ctctaaaatt ggtagcagag cctctaaaac actggtcttg cctgctccct
    13621 ctggtccctc aagagagact aaaaatcctt ttgacatgtc taactcattt cttttttact
    13681 acttctattc tatcaaaaaa ataggggttt gtgacaatct ttttgtcttc tcatttcata
    13741 ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggctgtactt gagtacggca
    13801 aggtgaagct gacgcagttt gaatttgatt ttcgaagagt atcaaccacc ataaaagagg
    13861 cagataaaac tacctcttat ttacctagtt cttgtgttcg tttgtaggct tgatgaactg
    13921 catccatgac tgttcctcgg aaagcatgcg cttctaggct tgctacacca gcgatagtcg
    13981 aaccgcctgg gctacagact tggtctttca atactccagg atgttgctga ctttcaagga
    14041 ccaattgccc agctcctacc acagtttgtg ctgccatttt caatgctatt tctcgtggta
    14101 atcctgtctg aacacctgca tctgccaagg cctcgataaa aagatagaca aaggctggtc
    14161 cacaacctgc aagacctgtc gctgcatcga ttaaactttc tcctagttca accaagagac
    14221 cagccttggc taaaagctga taaaagagct cactgtcctc agccctgcaa ttaggagaca
    14281 aggcataact aatcactcct tgcccgatag aagcaggggt attaggcatc atacgaataa
    14341 ttcggtgttg acttgggata agacttgcta gtttttctaa ggtcaatcca gctgccatcg
    14401 aaatcaaaag aagactttct cttttttcaa ggatggtctg gtattgagaa agcagttcag
    14461 aaaactgagc aggcttaact cctagaaaaa tcacatctgc ttctgcaaac atttcttcat
    14521 tgctggaagc ctgaccacca aagtctgcaa tgaaagcatc taccttagct tgactacgat
    14581 tggcaaggag aatctcatct gacgtcctag tctgcaagac agattttgcc aagctagcac
    14641 ccatattccc caaaccgata aatccaatct tcatctctta ctcccttatc tgcccatcac
    14701 cggcaaccac atacttgtag ctggtcaact ctttcaagcc catgggacca cgcgcgtgca
    14761 atttctgagt agaaatcccc atttcacaac caagaccaaa ttgtcctcca tctgtgaaac
    14821 gagttgaggc attaacatac accgctgcag agtccacttg atctgtaaag tatgctgcag
    14881 cttcagcatt ttccgtcaca atagcatccg aatgatgggt gctgtgggat tcaatgtgcg
    14941 caaccgcttc ttctaaactg ctcacaacct taacagcaag gacatagtct aaaaactcgg
    15001 tgtcaaagtc ttgggtctca gctgcttgac ctgaaacaaa ctggcttgct ttgctatcca
    15061 agcggaattg aattggttcc agtccagctt ccttacgctc tgcaaccagc atttgatcca
    15121 agcgaggaag gatgcttgct gccttgtttt catgaaccag cagaacctcc atggcattac
    15181 aaacagaagg acgactggtt ttagcattgt tgatgataga cagcgccttg tcttcgtctg
    15241 catccttatc cacatagaca tggacaatcc cagtccctgt ctcgataaca ggtacaatcg
    15301 cattctcaac cactgcattg atcaagccag ctcctccacg aggaatgaga aggtctagat
    15361 agcccttggc cttcatcata gcataactac tttcacggct agtatcctcc accagttgaa
    15421 tcacatttgg atgaatagta gtcgtctcca agcccttctt caaggctgtg acaatggcat
    15481 gggttgtttg ataggcatcc ttaccactac gaagaacaac cgcatttcca ctcttaagag
    15541 tcaaagcagc cgcatcagac gtcacatttg gacggctttc atagataata ccgatgacac
    15601 ctatagctac acgttttttt gtgataacca aaccattttc aagctgactt gtttctaaaa
    15661 cttcaccgat tggatctggt aaggcaacca cttcacgaat tcctcttgcc atcgcttcta
    15721 tacgatctgc atccaaataa agacgatcca acatcacatc tgagattttc cccttagccg
    15781 ctgccatatc gagggcatta gccgctaaaa tttcctcagt agcagccact aagtgatcag
    15841 ccatggctag caaggcttgg tttttcactt cttcactagc tgtgttaatc gattttttaa
    15901 cagcctgtac ctgttcaaat tgttcttgtc tactcaccat agtttacctc taaaattctg
    15961 taaaaagtag ttggatttca ggagtaatgg aaatccagtc gtcacggtaa atcaagacac
    16021 ccttggcttt ttgagaacgc aacatatcct ccaaagcaga tgctccaaat tgcacgcgtc
    16081 cttttccaag tgattttcca ctttccttgt caaatactgt cacgatatca ccgtaagaaa
    16141 aggctccttc tgcttcaacg ataccagata agagaagact ctttccatgt tgagagagag
    16201 cttccgcagc ccctttatca acccaaatag aaccttgact ctgagcatag aaggcaagcc
    16261 attgtttctg ggtacgaagc cccttctctt gagcaacaaa gtaagaacca tcctcggtct
    16321 cctctgccgc ctcaatcatg gaatctgatt tcaaggatga gcagatataa acaggaactc
    16381 ctgattccgt cgcgatagtt gcagccttga ttttggttaa cataccccca gttccgtttg
    16441 acgaaccagc tccaccagcc atatcaataa tctcacgatt gatggtctcg attctctcca
    16501 agcgtttggc tcttggatct gaattaggat ttccagtata gagaccgtcc acatctgtca
    16561 agaaaactaa aaggtctgct tggaccatcg ccgctacttg agcacttaga gtgtcattgt
    16621 ccccaacctt gagctcatca ataacgacac tatcattctc attgatgata ggaattgccc
    16681 cacggttgag caaaaccgac aaagcctgat gggcattttt ataacgacgc ttatccacaa
    16741 agtcatcttg ggtcagcaag atttgtgcag aaacgatttg acgcaagaga agattggtcg
    16801 tatattcttc caacaaaagt ccctgcccta ccgctgctga agcctgttta tcagcaattt
    16861 tagtcggacg ctttttaaat cctaaggctc caaaaccagc cgcaatggca cctgaagaca
    16921 ccaaaatcaa ctcatgacca gcctcgtgca gcatagccaa ctgctgggta atatccttta
    16981 ccttactacg tgataaactt ccatcctcat ttgtcagaga agaagtaccc accttaaaga
    17041 caatccgttt gtatttcata gtctcactcc gattattcat atttatccat tataacatga
    17101 ataacaggaa atagaaaaga gttgatagga aaaagaaggc cctaaaacct tcttttctac
    17161 tactgttctg attccgattt gtttacactc gtttcagagc tttgtgaagt tgaggatgtc
    17221 aaatcttgac tagttgaaaa atttgtaaca ctttcatgtt gactcgtttc tttattttca
    17281 agatcctttt tcactacttc tttggattct tttatttctg aatgacttgt agttttaatt
    17341 gtagcagatt tctcgatcga aacatccact aaccaggcac cacttgaatc cacagtataa
    17401 ccttctggtg tcttaccgtt cacaagtaat tgaccatttt ctttcaagaa gtaccattta
    17461 tccttgtcct tgatccaacc cacagctcgt gaaccgtctg tcttgtagaa ataccaatca
    17521 tttgcctttt tatgccagcc ttgcttcata gctcctgaat cttccagata ataatgttga
    17581 ccagcaactt ctatctcacc tgtcttcatg acaccagtag aatccatata gtaccaggtt
    17641 tctttatctt ttacccagcc cattttcatt tcccctgatg gagcaaagta ataccattgc
    17701 cccatatatt gaagccatcc tgtcttcata gaaccatcaa catccaaata ataccactgc
    17761 tctttatctt gaatccaacc aatttccatc tggttttctt tgttaaaata ataccagatt
    17821 ccagcgattt tcttccaatt tttagcagaa gcaccagagt ctgtcaaata gaaccaatga
    17881 ttgttccatt ttttccattg attctgtaac aagatacccg tttggttaaa gtaataccat
    17941 tcaccttcga tttcattcca accgacagca tactctcctg tagaatcagg cgcttgatac
    18001 caccagttcc catatgcact cttatgccaa ccagcttgaa aacttggaat cggcttgtag
    18061 gatgttgaaa tattgacaaa accgtctttt cgaatatcaa aaactgttgc atcatagtct
    18121 ttgctggctg cgttgattct ctcaattcct cgttctttga gccaattaac atactcacta
    18181 tcaacaccat ttttccaagg tagactatcc gaagtttgaa caatcaaact cggactcaaa
    18241 tttttaatga aatccttggt atttgatttg ttggtatcat ggtgatgatt aaacttcatc
    18301 aaatcaactt ttccaatgag aggaccatac ttgtcttctg ctccatgaac attatctaaa
    18361 tcgcccccaa ggtaaatttt cttgccattg actttcacca cgctaatcaa ggaattggaa
    18421 ttgtcatccc aaattttctt taattcaccc gatgaatcag tttcattttc ataattatag
    18481 agctgaatat ccatgtcccc aaactgaaaa tgagcatccc cttgtgtgat attttgaata
    18541 actgaaacac ctttttctgc agcagtctgt aaaaccttat catagccata cagattatcc
    18601 catagacgtt cagaattagt aatacgacta tcactatatt tcttaagata gactcggtca
    18661 actggatagg tagacagtaa ttcatcaaca tttccaatat gatcactgtg ggtatgggtc
    18721 accaaaataa aatcaagttt ttggacaccc aattccttca aacgacgaaa gacacggtct
    18781 gttagaacat gcttataaga cgtttcaatt ccttctctcc atggatagcg agaatcactt
    18841 ccatctggga aatcataatc ttctcctgta tccaccatgg caaaatgtcc attgctttca
    18901 agaataatcg catcactgcc accttcttga acattgataa agtggatttt atttcctgaa
    18961 ctttcttgag cctgaacatt cccataccat gacaaaccta aaaaagcgcc tacaagtgct
    19021 aaactagtta atttcttttt cattcttact ttctcaatct ctccaaggtt tccttaaaaa
    19081 tctctgggat atctgcttta aattccaagg tcttacctgt tctcggatga gtaaaaccta
    19141 aagtcttggc atgaagaaat tgtccatgtc ctttcaaagt cttgcgagga ccatagacct
    19201 catcaccagc gactggatgg ccgatataag ccatgtggac acggatttga tgagtgcgcc
    19261 ctgtctccag ttgcaactct actaagctat aatcgccaaa gcgttccaag acgtgaaaac
    19321 gcgtcactgc aggcttccct ttagcagtta cagcctgttt cttacggtct ttttcactcc
    19381 ggccaatcgg cgcttcaatt acaccacgat cattaggtag atttccatga acaatcgccc
    19441 aatatttgcg gagagacttt ttatccttga gttcttgggc aagtgctaga tgcgcatcat
    19501 cgtttttagc aatcatgaga agacctgacg tatccttatc aatacggtga acaatccctg
    19561 gacgcagaac cccattgata cccgacaagt ccttaatatg atacatgagg gcatttacta
    19621 gggttccact ggtatgacca gcactcgggt gcacaaccat tccctgaggt ttgttaacga
    19681 cagccacatc ctcatcttgg tagactattt ctagcggaag atcctcagcc acatactcta
    19741 atacctctgg ttctggcaca tggtaagtga cgacatcacc ctcttggact gtgtatttag
    19801 ctttcttgac ttgaccattg accaagacct ggcctgattt aatttgttca ttcgcgagac
    19861 tacgtgataa ttctgacaaa tctgacaaag ccttatccaa acgcagacca ccagtttcaa
    19921 ttttaatttc catttatttc ctcttttagc attgcaatca ataaaataat cactccaacc
    19981 gtcagatagc tatctgccac attgaaaatt gcaaagttga taaagtcaag gtggaacata
    20041 tccacaacaa agccctgact gaccctgtca ataaagtttc caagaccacc cgcgattatt
    20101 agagtcaaac ccaagaccat ccagaatgag tcctccatgt gtttatgtaa ataccaaatg
    20161 gcacctatca cgacaaccag agtaatgaca gcgaataaca actgctgatc ttgtaagata
    20221 gaaaaggctg cacctcgatt ttgcaggtag gtcaagctaa cgaaattggg gatccaggag
    20281 cgcacttcac ccagtggaat ctgctggacg atataggatt tgaccaactg atccagccca
    20341 atcaaaagca gtacaatgac tgccactatt gctctttttt tcatgatttc ctcttttgat
    20401 caaaatattc ttgcatgact tctacgaaga gagtcccagc ttgactaagc tccacttctt
    20461 cacgtttaac atagaccatg cggttatcta ggttatcctt gagacgaata actgtaatgc
    20521 cattaacact gtcactatct aaaaatccag aacctgtcgc ataggcgtcc gtccgctcca
    20581 aaataccatt caaggtggca cggtctgtca cattaaacat ctgtgagcta gcgctggtat
    20641 caacaaagtt ctctgaataa taaaggtact cgtctttctc ttgagtgaaa cgaaccgttg
    20701 gtaaatccgc taaatcctcc atgactaatt cctctttctg ggctaaagga tgaccctcac
    20761 agagataaat atgggtatgg aaaggaatca attcgatgac ctccagacct aacttttcaa
    20821 cccgttgcat aatccccttt ttattttgat tgttgaggta gataatccca atctcactat
    20881 gcccttgcgc cacttcatct aatatttgaa cagtagttga ttcaaaaata cggaagttct
    20941 tatagtcagg atagcgctct gaaaaggccg taatagttgg tggtaagaag tcatagtgct
    21001 ggctagcaac ggaaaattca tctttttctt cttcaggatt ggcatactga ttttgaaaaa
    21061 tatcaaatcc tttaaccaat tcttgcgctt tttcataaaa ttccatccca cgacgggtca
    21121 agaaagtccc tgagctggtc cgacggaaaa tcttaaaacc caactctttt tccaaatccc
    21181 gaacagaaat agacagactc ggctgactaa catacatctt ttcagcagct tcacgaaaag
    21241 taccactatt ggcaatagcc acaacatagc gtaattgttg aatgttcatc ttctacccct
    21301 aacttcttta tcttttcatt ataccatatt ttagaagttt tccaaaaagg aaaaaagaac
    21361 atcctattct tcttaactat cttcactatc tgcctttctc acgccaatct tattttcaaa
    21421 atcaattcaa aaaaataagt ggctacacac cacttcagta tcggaaagaa aaacgttgac
    21481 tatttgtgaa aaaagaaaat gccggaaaat tccgacattt ttttagtaag ctaacttcct
    21541 gaaaatagga gtaaccaaac atcccagaag gcagtgatat tgatgagaat atgaactagt
    21601 attggatagt agagattttt agtcatgcga tacaatagag ctaaaatcag acctccacta
    21661 gcataaatga aaaaagcaag aggagttaaa gcaaaattga tatgaatata accgaaaata
    21721 atagcagaaa gcaaaacatc tccgtaccaa ggtgagtttt tgaaaaaggt tgtcataagc
    21781 acacctcgat aaatcaattc ctcagcaata ggggcgatga agcaaacgat gagcaagaaa
    21841 taagggaact cctgtctccc catcatttct atcgtttcat tcaaagaaat ctgatttgaa
    21901 gacagggata tgaaatacga aaagaggaag tcagacatat atgaaatgat gtagcccagt
    21961 aaaaggtaga tgaagtacct cagctgccac ttgaaatgaa aaattttctt tcctgcaaaa
    22021 accagtagat agatgaccgc taagaaaaga actccgctct ccatcaaaag cagaatctga
    22081 aaaaattcac gacttgctgg tagatagggc tttgcgaggt gattgaaaac tctccaaaac
    22141 caagttgatt tgtaaaaaat taaggacaat gctagtaaaa tttgaatagc ccgttttttc
    22201 atattaaatt ctctgctttc tccccttgtt tcttcatatt actaataaat tttacttaaa
    22261 atctcgcagc acttcctttg caaagatgat tgcctcctcc aatatatcct aaatcatagg
    22321 tgcctctggg acaaaatcca tgaaccggca gagttaatac tggttctatt tcccaatcgt
    22381 acctaccatt aggattttta ccatatgaat ctgctccacc ttgtatcagc tctaattctt
    22441 catttgttag atttatattt tttgtcatta aaatcatgat atatttcctc cattattctt
    22501 cagattagtt gagtaatctg atactcatac ctacttacaa aaaaactatt atattaagtt
    22561 ggttttttaa tttgtctagt tgcaacattg acaaacatag tatagcatat ctttgcgaaa
    22621 tatcctcttc aaatcatgaa ttgtcatcaa aacatcttaa actataaaat caattagtct
    22681 caagctttct atcaatttct tctccaaaat atgctataat aatagcaaaa gataaagaag
    22741 gaagacctat gattaaacta ctagccttgg atatggacgg caccctcctc aatgaagcca
    22801 aggaaatccc acaagctcac attactgcta ttcacaaagc cattgaaaaa ggtgtcaaac
    22861 tggttctctg tacgggtcgc ccccttttcg gtgtcctccc ctactacaaa aaactgggac
    22921 tcgacctcca gaatgagtat gtgattgtta acaacggttg ttcaactcac cagactagcg
    22981 actggggctt ggttgactgg caagaactta gtccagctga catcgaatac ctctatgacc
    23041 ttgctgaaaa gagtgatgtt cagttgacac tttttgacga gtcacattat tttgttctcg
    23101 gtggaaagcc caatcaagtt attgaaaatg atgctaaact agtattttca gacctgactg
    23161 aaatttctct tgaggaagcg actagtggaa agttccggat gttccaaggt atgtttttag
    23221 gaacaaaaga acaaacagac gattttgagc agcgttttgc tgaagagctt tgccaacgat
    23281 tcagtggagt tcgttcgcag cctgtcattt atgaagcaat gccacttgga acgacaaagg
    23341 ctactgctct ttcacgacta gctgagattt tgaagattga ttcctcagag attatggcca
    23401 tgggcgatgc taataacgat atcgaaatgc tccagtttgc agggcttggg attgcaatgg
    23461 gaaatgccag cgattatgtc aaatctcttg cggatgccgt tacctcaagc aacgaagaag
    23521 acggcgttgc gcgtgctatt gagaaatata ttctataaaa aagaaaagca aatagacaga
    23581 agttactgct atttgctttt ttgctaatat tttaaaatac ggactaatca atagagaaga
    23641 atagcgaatc ataaaaatcg atttactaga tgccatagta tcttatagtt gctaataaga
    23701 agttagtcta gcagaatgaa tctctcattt ctcccacact aatctgtaat ttgtctatac
    23761 atttctggtc ttctatctcg aaacaagccc cagtttaggc gttcacttgc tcccttgtct
    23821 aggttataag tagctaacag aacagcttct tcttgtcttt cagctcgttc tagaatagct
    23881 cctgtttcat ccgtcataaa ggaggaaccg tagaagtcaa gactggaact ctgtccgcca
    23941 ttttcctcac taggagtaac ctcctctaaa ccataacgat tggctgcgat gactggaaca
    24001 atattcgctg ctgcgtgccc ttgcatagta cgttgccagt gaccacaact atctgtatcc
    24061 aaaatcggct ctgaaccgat ggctgttgga taaaagagca attcagcacc attcaatgca
    24121 agacagcgcg ctgtttcagg gaaccattga tcccaacaga taccgatacc aatcttagca
    24181 tagcgagtat tccagacctt gaaaccagtg ttaccaggcg tgaaatagaa tttttcttga
    24241 taataatggt catctggtat gtgggtcttt cgataaacgc ccagcacttc cccatctgca
    24301 tcaatgacgg caatagagtt atacaagaca ttaccatctt tttcatagaa actgattggt
    24361 aaaacaactt gtagttcctt agcaatcacc ttaaaatgct gaatggcagt attttccgct
    24421 acagattggg catactggta gtagtcatac tgacgttcct gacagaaata gggatgttca
    24481 aacaactcgg gcaagagaat aatttgggct ccttgctcag cagcctgacg tactaaacgc
    24541 tctgcggttt ggatatttgt tgccacatcc ttagcgcatt gcatctgaat ggttgcaact
    24601 cttacatttc tcatcttttt ctcctattct ggaatttgtt gggtgataca gtggatattg
    24661 ccaccaccta agagaatatc tctggctggt attccgacaa ctttacggtc tgggaaacac
    24721 ttgctgagga tatctaaggc cacttggtcg tttacatcct caaactgtgg aaccaagaca
    24781 gccttgttgg cgatataaaa gtttacgtag gaagctgcta gtcgttcacc tgcgtatcgc
    24841 ttttcttctc cttcttcata gatgtagcct ggcaaatctt cttctgtcac aacttgtcga
    24901 actgcaggga taggcaattt atgaatggtg aagtgacaac cttttgcatc tgtttcctgt
    24961 tctaagagtt cgagatctgc ttttgacatg gcatactggg gatcattttc gtcatctgtc
    25021 caagccaaaa caagctcagc aggaccaaca aaggcagcaa cattatcgac gtgttcattg
    25081 gtttcatcct gataaatacc ataaggaagc caaataactt tttcagcacc aagactttct
    25141 aataatgtgt tttcaatctc ctctttagtc aggttaggat tgcgaccagg actaagcaag
    25201 caactttcag ttacgagaat agttccttga ccatcgctat ggattgcgcc tccttccagt
    25261 acaaaaggtt tagcatcata gacaggcctt tccaaggcct cagcaaaacg actggctact
    25321 tggtcatcct cttcataatc ttgataaaga ccatcatagg tgcctcccca agcattaaag
    25381 gcccaatcca cggctaattt cttaccttta tcattgacga gaatggttgg gccagtatca
    25441 cgcgcccagg catcattggt gggaatgtct aaataaacaa ccttgtctcc aagataggat
    25501 tgggcttcag atagataggc ctgctccacc aaaagataga ctctttcccc ttctgctatg
    25561 gtctcgatga tctgagtaaa tgctctttta gcagcctttc cttgaaaagg ccatgatcct
    25621 ggtcgagtcg gccatatcat gagggtacca tgatggggtt cgtactctgc tggcatgtga
    25681 tagcctaatt tttttggact gtccatcatg ataatctccc tttaaagtct tgatagccaa
    25741 aagctttgag taagctacag tctccctgtt cgtccatgag atagagactt ggcaatccaa
    25801 taccattaaa ggtattattt ttgacaaaag aataaatggc catgtcttga aaataaagtc
    25861 tgtctccgat ttggactgga ttttcaaaac tataatcacc aatcacatcg cccgtcagac
    25921 aggtattaga agaaagtctg taggtatggg ctttttcctg tgactcaaag ccatttctca
    25981 aaggtggacg atagggcatc tcaagtacat caggcatatg gcaggtcgca gaggcgtcta
    26041 aaaccaagat ttccataccg ttttctacaa tatctaatac ctcagttgct aaataacccg
    26101 cattaagcgc aatggcttca ccaggctcga tatagatttc aagattgtaa gtttttcgga
    26161 tacgcttgat ttctgaaatc agcaaatcca catcgtaacc ttctcttgta atatgatgac
    26221 caccacccat attgagccat tttacctcat gtaagtaggg accaaactgt tcttctactg
    26281 ctttcaaagt tgtttgtaaa tcatctgctc cctgctcgca aagggtatga aaatgaagtc
    26341 cgtcaaccaa atctagcaaa tcactcggaa tcttgtctat agtaactcca aagcgagaac
    26401 ctggtgcaca agggtcatag agcgcgtgat ctccttgagt tgaacactga gggttgaggc
    26461 gcaaaccaac actgacacca gcctctcgac aacgcggacc gtgtttacgc aactgtctct
    26521 ctgagttaaa gacgatatgg tccattatct ctagcaattc ctccaagtct gcatccttga
    26581 aagcaggcgc aaatacatgg acttcaccag gaaattcttc ccttgccaat ttggcctcat
    26641 agagtccact agctgtcgta cctgatagat actggctaat caagggataa gttttgtaga
    26701 gggaatatgc cttctgggca agcaagacct tgcaaccggc ctcttcttgt acatattgta
    26761 gaatgcggca attagcttct aacttggcca agtcaataac ataggctggt gttggtactt
    26821 gttctaactt cattagtcca ccatttgtgg attttcaacc acaacccatg gcaaaccata
    26881 ctcattcaaa gcttccatga atggatctgg atctaactcc tcaaggttat acactccagc
    26941 ttgtttccaa gttccgttca tgactaattt tgtcccaatc atggctggaa ctcctgtcgt
    27001 ataagaaata gcttgcgaac caacctctgc gtaacattcc tgatggtcgc agacattgta
    27061 gatatagata gtctttttga caccgtcttt gacacctgta aagatacatc caatattggt
    27121 ttttccgact gtacgtggcc caagactggc aggatctgga agcaaggctt tcaaaaattg
    27181 aattggaaca atttcttggc cgttaaagtt aatggtatcc gtacgaagga gtccaacatt
    27241 ttcaagacat ttcatgtgcg tcaagtaaga ttgaccaaaa gtcataaaga aacgaatgcg
    27301 tttgacacct ggaatgttct tggccaatga ttcgatttct tcatggtgaa ggagatacat
    27361 atctttttgt ccaacttgag ggaaatcata ctcacgcttg atagacatag cttcgacttc
    27421 gacccatttc ccatcttccc agtaagaacc tggcgcagaa acctcacgga gattaatttc
    27481 tggattaaag ttggttgcaa atggataacc gtggtcaccg ccattacagt ctaaaatgtc
    27541 gatataatgg atttcatcaa aatagtgttt gagggcataa gctgaaaaga cactagttac
    27601 acctgggtca aaaccagaac caagaagagc agtcaagcct gcttctttga atttctcttg
    27661 ataagcccac tgccatgagt agtcaaagta ggctgtaaaa ccaagttcct tacaacgttt
    27721 ttcgtagata gcacgccact cagggtcttc tgtgtcttct gcttcgtagt tggctgtatc
    27781 gatatagtga acacctgttg ccaaacaagc atccataatg gttaaatctt gataaggcag
    27841 agctacattc aaaacagctt ctggtttgta gctttcaatc agggcaatca cttcttcaac
    27901 cttgtcagca tcaagtgctg cagtttcaat tttagtactt gttttgcctt ctagcttcgc
    27961 tttcaagtca tcgcattttg acttggtacg gctagcaatc ataatctctg taaatgtttc
    28021 gctatcttga caaatctttg aaatagcaac ttgggcaacg cccccacaac caataactag
    28081 taaacgactc atttttttcc ttcctcttct tctaaaatgt cctcaacata cttgggcaac
    28141 ataaaggctc ccacgtgtaa gtttgcagtg tagtattctg tgaaaagctg gcgttttttc
    28201 cagccttcct tgtcaaaatc tttgacaggg tggtattttt tcgatgcaaa tccaaacaac
    28261 caatagccag ctgggctagt tggaatatgg gcctgataaa cccgactgat tggaaaggct
    28321 tgattgacct tgcggtgcat gcttcggcag gccgactcat cctcgtcaaa gaagggactc
    28381 ccatgctggt aaatcatgat gccgtcttcc ttcagagctc gataactatt gccgtagaat
    28441 tccttggtaa agagtccttc cgtatggcca aatggatctg tcgcatcgtt gatgataata
    28501 tcgtaatcat cttcgcagtt tcgcaaaaag cgtagcccat tttggtagta aatggtaaca
    28561 cgaggatcat ctagccctgc agcaaagtct gggaaatact cacgacagac ctcgaccaac
    28621 atctcatccg gttccacaat atcaatttgc tccagttcag gatagagggt taatacttgg
    28681 gcaacaccgc cgtcaccacc cccaataacc aatactttct ttggatttgg gtggacagcc
    28741 atgggaacgt gaacggtcat ttcattgtag acgaaatcat ccgcatctga gaacaagaca
    28801 tggccattta aaatcagtat tttcccaaaa gctggcgtat ccaagacttc gatatcctgc
    28861 cattcacttt ttccagcgta aagttgcttg gctgttctca gagacaattt gacatctgga
    28921 gtatgaactt cagaaaacca taaatccatc tatttctcct tatattatat ttagcgcagt
    28981 tagctactgc caaagcccaa gtggtatact tggaataagc cactgtggat tagttcattt
    29041 cctccaatcg cttgacgatt tccgaaaggc tctaaccaca gcttttgttt caattggtaa
    29101 tgagtttgat aacgcataga cgttttatct cgtctaaggt aacaaggcaa aaagttcgtg
    29161 gataaccaag tagacttgaa atactacatc tggaggtact cccatgcttt atgtgggcat
    29221 tgatatcgct aaaaataaac acgatgttac agccttgaat gttcaaggaa aaactgttct
    29281 taaaccactc actttttcaa ataataaagc tggttttgaa ctcttagatc tgtctcttcg
    29341 acagctcaac caagactgtc tcatcgctct tgaagatact ggacattatg cctttaatct
    29401 actgaacttc ctgcatgaac aaggatataa agtttacacc tacaatcctt tgctcatcaa
    29461 ggaatttgcc aaatcattat cacttcgcaa gactaaaacg gataagaaag acgctcatgg
    29521 catcgctctt aaacttcttt ctgaccccaa tcgtgaacaa tttcaacacg ataatcggca
    29581 agtagaacta aaaatactgg ctagacatat tcatcgtctc aagaaaaaca gtctgattgg
    29641 aaagtacaat acactcgttg tcttgatatc atctttcctg agttggataa aatcgttgga
    29701 aagcattcag aatataccta ccaactcttg acacgctacc ctaatcctca gaaaaggatt
    29761 gaggcaggat ttgataagct gatagaaatt aagcgattga ccgcttctaa ttcaagggct
    29821 ttttgccgtg ctctaaaatc atcgccaaat catcaaattg aaataagaaa tagtttagat
    29881 aaaactattg acttttctta tagtttgtct ttctcttaat aacgttgata tgattgacct
    29941 ctggatcttc cgtcccttgg agggagcaac cacgttcctt ggcgaattgg atatagtcta
    30001 caatttctcg tgtaatgcgt tcaccaggag ccaagatagg aatccctgga ggatagcaca
    30061 tgacaaattc tccacagacc tgtccaacag actcatctaa ggtcaaactt tttctctctg
    30121 aatagaaggc ttcttgtgga gacagcacca actcgggctg aatatattct ccagctatta
    30181 agtccttccc atctcgtgaa tagagtctct taatatcagc cagagcacca accaagcgct
    30241 cgatgtcttg gatgcggtcg ccgatggaaa tataggccaa gatattgccg atatcaccaa
    30301 actcgatctg aatgtcgtat tcgtctcgca agaggtcata aacctcgata cctgttaagc
    30361 ctattccctg agtgtaaact gacaacttgg ttacatcaaa atcacagacc gacacaccat
    30421 ctattaactc ttttgagtaa gcatagtaac cgccaatggc attgatttca tgacgagcgt
    30481 actcggatag ctcaatgact tcctcaaacg actctttacc acgaagggcc aagttgcgac
    30541 gtgaaatatc caaactagcc atcaataagt aagaggcaga tgtagactgg gtcaggttta
    30601 taatctgacg aacgtactcg ggattcatct gctccccgat taaaagaagc gaactttgtg
    30661 tcaaactccc accagactta tgcatggaaa ctgctgccat atcagctcct gcatccatag
    30721 cagaaattgg aagtttacca gtgaaatgca aatgcgctcc atgggcttca tctactaaaa
    30781 ccatcatgcc agcttcatga gccatttctg tcaacccctt taggtctgaa cagatgccgt
    30841 agtaagtagg attgttgatt aggatagcct tagcatctgg atggtccttt atggcctgtg
    30901 ctactcggtc attttcaaga cctaaagcga taccaatctt aggatctaca ctcatctcga
    30961 tatagatggg aatggcacca catagaacca acgcattgat agcagattta tggacatttc
    31021 gtggcagaat aatcttatct cctgccttgc aggttgccag aatcatagtc tgcaccgatg
    31081 aagttgttcc accaatcatt agaaaggcat ggctagctcc aaaagcatct gcagccagct
    31141 cctctgcatc acgaataatc gaaataggat ggcctaaatt atccaagggt ttcatcgaat
    31201 tgacatcaat gcctacacat ttttctccta agagttcgac aagttctgga tttccccgtc
    31261 cacgcttgtg acctggaaca tcaaagggaa caatcctttt cttgcgtaac ttcaccaagg
    31321 cttcataaat tggggcttgg ttttgatcta actctttcaa aacatactcc tttcaatatt
    31381 tttaggacct tatgaaaact aaggaactaa aggttgactc atgaaaaaag agcaggtttt
    31441 cacctgctct gcaatcttct gacgtgttta ttttggtttc tgttgagtat gtctgttccc
    31501 gatgtttcct aactctctag tagcaaatat tacgtacttt attgatcgtt tcacaagatg
    31561 acgtgtgctt tcaccacact aaaaatttat gaattaggac aagtgtccta acatcaactt
    31621 ataaaagtag gcttttaacc ctatcaataa tgtggctttt caaccacgct tcggcattca
    31681 accctgcctg tccgtaggca ttttttactc gggtttatat ttgctagaaa acatcatctc
    31741 attttctaag ctttattact atatcatgtt tttaagaact tgtaaagtat tttttgataa
    31801 aaaacaaaaa tatagtagat tgaaactaga atagtacact tctgcttcta aaacattatt
    31861 agaaatagat tttactgtcc tgatcgattt gttctgttct tgtttcattt tactatatgt
    31921 tcgtaaatga aagttctata atgaagttag ccacccagcc ctcctaaaaa ttagatggtt
    31981 caactagctg atagctcttg taggcataag aaaacacctc tgtgttatac ttgttgttca
    32041 accacaaaca caagaaaggc acagagatgc aagacaatta tactacaaaa gccaaacatt
    32101 tgacaatcga tagccgtcgc ttaatcgaaa gatggaaaaa agaagggaaa tcaaatagag
    32161 aaattgcctc tctgcttgga aaagttcctc aaactatcca cactgaaatc aagcgtggga
    32221 cagtccgaca atgtcttgga aaagggcgct tcaaagaggt ttattctgcc gactatgctc
    32281 aacagtctta tgaaaacaat cgcaagcgct cggtcaggaa atcaagcgtg accaaggaac
    32341 taaaggaaaa gattctccac tatcataacc aaaaattttc gcctgaaatg atggtgatgg
    32401 ttaaaggggt taacgtggga atttcaacca tttactattg gattcatcat ggaaaattgg
    32461 ggttaagcaa acaggatctg ctttacccta gaaaaggaaa agcgcttaag aaacaggcta
    32521 gcaccaactt taaatctgct ggtcaatcca tcgaacagcg gtctgaagct atcaatcttc
    32581 gcttggagaa tgggcattat gagattgata cggttctact tacgagagcg aaaaactact
    32641 gtttgcttgt cttgacggat cgaaagagta gacatcagat catccgattg attccaaata
    32701 aaagtgctga tgtggtcaat caggctctaa aactcatctt aaaacaacac aagattcttt
    32761 ccatcacggc agataatgga acggaattca atcgcttgtt tgatgtattt tctgaggagc
    32821 acatctatta tgcgcacccc tatgcctctt gggaaagggg aactaatgag aatcacaaca
    32881 ggctcattcg tagatggtta cctaagggaa tcaagaaaat gactcccaaa gaagtcgcat
    32941 tcatcgaaaa gtggattaac aactatccta aaaaatgctt ggactacaag tcacccagag
    33001 aagacttctg gatggctaac ttgaacttga aatttagcgt cagaaacaaa tcacgaaatt
    33061 gattcaaatt ttgcaggtcg tttaaatatc ctgcgtgcgg gtgttcttga tgctaacgat
    33121 ggaattattt ccattgctgg tgtggttatc ggagttgcca gtgccacgag caatatctgg
    33181 attatctttt tatcaggttt tgcggctatc ttagctggtg ccttttcaat ggctggtgga
    33241 gaatatgtat ccgtttcaac tccaaaagat accgaggaag ctgccgtttc gcgagaaaaa
    33301 ctcttgctag accaagatag ggaactagcc aaaaaatccc tctatgcttc ttatatccaa
    33361 aatggagaat tcaaaacttc tgcccaactc ttgactaata agatctttct taaaaatcca
    33421 ctcaaggctc tggtagagga aaaatatggg attgagtatg aagaatttac caatccttgg
    33481 cacgctgcca tttctagctt cgttgccttt ttccttagaa gtttgcctcc aatgctgtca
    33541 gtgaccattt ttccaagtga ttaccgcatc cctgctaccg tccttattgt cggtgtggcc
    33601 cttcttctca ctggttacac aagtgctaga cttggaaaag ccccaaccaa aacagctatg
    33661 attcggaacc ttgctattgg tctcttgacc atgggagtta ccttcctgct cgaacaactt
    33721 ttcagcattt agaatacaag aaatacctcg attttgaagt cgaggtatct tttttacatt
    33781 tgcacaatct tgcgataact tcttgaagta atcataaaaa tcagcacata ggcgatgagg
    33841 aagatagcgc agatagacaa ggtcacaatc aacatcatag tcgtatccag tacaccaatc
    33901 acttttaaaa tcaggctaag catatggtag gcaaaggcta gatgtaggaa ggcaaagagc
    33961 aaaggaagga agaaaacagt taaaacctgt ttgttgatgg tttgcttgat ttgcttttgg
    34021 tccaaaccga ctttctgcaa gataataaag cgttcacggt cttcgtagcc ttcagaaatt
    34081 tgtttgtagt agatgaccag aacagttccg accataaaga taatggatag gaaaataccg
    34141 ataaagaaga caccaccaaa gagggcactc atctgagcac tagcatctgc tagagtgcta
    34201 ccataaacat agctaccttc tgtgtctaat tgagcattaa attgattgag gtagttttca
    34261 tactcctcag cgaccttgag ttgttcttct tcgctggcat ctacattcat accaccgtaa
    34321 aactgattat agatagccga atctgggaat tgatccaaaa aggcttgtaa atcaggaaca
    34381 acaaggtaat tgtaatcagt agtcaagata ttaaacttat ttggaacatg gttgacaatg
    34441 aaatctttat taaattcttc tttgacagaa aattgatgat catttagagt tagagctttc
    34501 tgtcctttca gtccgtcatt tttggcaaag agaccgacct catttcctga tagagacagt
    34561 ttttgaccag tcatattttc ataatctttt tggtcaaata ccatgaaaac tgtttttggt
    34621 tggacacggt tttgtccttt ttcaaaaata gttaacttgg ttccttcttg atttgcaata
    34681 ccaaagttac tgtaacgaag cacttctttc tctttaatct tataaccttt gtcacttgca
    34741 aactggctca agagtttgtc caaatcttct ttttcaacat tttgccctga aaccccaaaa
    34801 tcatgaggat ttagaacttt tttaaaggat tctgcggaat tgaaaatgct tgtcgctgct
    34861 gacatggtta ccaaaaccat tgttgacaaa atagcgatag ttgctagtcc aactgcattt
    34921 ttcttcatac ggaaaatcaa gttagaaaca gatatgagat tattaggttg gtaatagtat
    34981 ttcttatttt tctttaagat ttgaaggaaa acggtaatcc ctgcattgaa caaaagataa
    35041 gtaccaaaga taaccagtaa aacagctatg aagaaggttg ttaaggctgt aagaggatct
    35101 tttaccgtaa gggcaagata atatccaatc cctaaactta tggaaccaag aatagtttgg
    35161 agaggaagga agcgaccttt tttctctccg ctagctttct cacgtgagag ctggagggca
    35221 ttcatacggg cgattcgaag ggcattcagg aacatgaggc ctaggaaaat caaaccgaag
    35281 acaagaagta ctgtaatgac aactttcatc tggaaggtag cgaccagttc caccttcaat
    35341 ttcatcagtt tgagtaggaa agcgaaaatc aacttgtcaa acaaggctcc aataccgata
    35401 cccgctccaa cagttagaat cccaaatacc actaactcct taaaggtcat actgataaga
    35461 tgacgcttct ccaagcccaa catgccataa attcctagtt ccttggaacg gttcttcatg
    35521 acaaaactat tggcatagag aacgataatg gctgacgcaa gggtgacgac aaacatacca
    35581 aatccaagtg tagcctgaat ggttgttcct ccacggattt ccgcaatctt aggattgaag
    35641 gttagagagt aaaagagata ggtgacagtg actgccaaga gaacagccag cgcaaaagga
    35701 tagtagagtt tgcggttttt aatcaagttc gataccgcta acttattggt taatcgaaac
    35761 atactaattc acctcgcttg ccatgacagt caaggtatca gagatttctt ggaacatctg
    35821 acgttctgtc ttctctccac ggtagatttg attgtaaaga atgccgtctt tgataaagag
    35881 tacacgttta gctctgctag ctgctgctgt tgagtgggtt accatgagaa tggtttggcc
    35941 gcgctcattg atttcatcaa agacatcaag taaggctgca gatgacttgg agtcaagagc
    36001 tcctgtcggt tcgtccgcaa ggagaatttc aggttctgtg atgatggcgc gggctactgc
    36061 tacacgctgt ttctgaccac cagaaatctc gtaagggtac ttctcttgta attggtttat
    36121 tcccagattc tcagctgtca ccaccaattt cttcatcatc tctgtaatag gtcttcttga
    36181 caagacaagc ggaagcaaga tattgtcctt tacagataga gtatctagca agttaaagtc
    36241 ttggaagaca aagcccaact tttcacgacg gaagctagag gcctgtgaat ttttaatggt
    36301 tgcggtgtca gttccattca agtaaacctg accacgactt ggtttatcca gcatagctag
    36361 aatattgaga agagttgatt taccagaacc agactcaccc atgatggcaa cgtagtcacc
    36421 cttttcgacg gtaaagtgaa tatccttgag ggcttctact tggttgccct gaaaacgtgt
    36481 tttataaatt ttttgaacgt gttttacatc taaaagtgtc atgagaatct cctttcttaa
    36541 tatcccatca aatgaaatgt agcttgcatc atagtgcatc ccagctgaac gcgctcaatt
    36601 tctttgtgac ttggttttct agttttctta tgtaagattt gtttcatgat gttactcctt
    36661 tgttctttat gagtctagtt tacatcaaaa aaagaccgct tgccataacc taaccttaca
    36721 aaagagactt taatcttaca tttttgtaag attgggtgaa atataagcaa aattacttag
    36781 aaaatgattt gatttcctat ttttaaagtt gtaaaatata agtgtgaaaa ggcttacttt
    36841 gaaaaggagg aagcgggaac taacatttta gtaaggttac ctagataaca taccattcaa
    36901 tttaacactt gcaagtcgtt caacctataa ggaaagaagg tgcgctttaa tgaaacacaa
    36961 agaacatata ctcatcggac ttctctatct gctctctcca ttcatcggtc agctcttagt
    37021 tgaacacaca cactttatcg gtacagaatt taccggcact gcttatgtta tttgctggtt
    37081 atcagttgtt atcagcatcc accatttttc caaaaatgta ttgtctcaac agcaaaaata
    37141 gattaaaaaa ctgcgtttaa attactgctt ttatagtaaa atgaaataag aacaggacaa
    37201 atcgatcagg acagtcaaat cgatttctaa caatttttta gaagtatagg tgtcctattc
    37261 tagttccaat ccactatagt ttgtggatga agctggcgaa atattattaa tactattcaa
    37321 aaatcaaagt gcaaactata tcaacttcac attgccgact aaattacagc ctgcggctgg
    37381 cttcctagtt tgcactttga tttttattga gtataaaagt aaaaaagctt taaatcaagc
    37441 agtttagact attgatttaa agctttttgc gtattccaaa tcttttgtaa aacctcacca
    37501 aaaccagcct tcattatcgt caatggcttg agcttctttg cgtttacgtg ggcctgttcc
    37561 gtacatttct gcttcgattt cttccttggt tggcatgata gaagctagga tgatatagat
    37621 aatcacccca agtccaaagt ttgcgactgt aaaaatggca aacagaaaac gtacaagggt
    37681 aacatcaaag ttccacttgt ctgacagacc tgctaaaact cctgaaatca tgcgatttcg
    37741 tctcatttta taaaacttac tgttcatgat atttcctctt tctgctaggt tagactagta
    37801 tatcacggat aggctcggct ttcatcagtc ctcaggctga tttactaata gcaactttcc
    37861 tcgacaaagt ccacagcgat agcgttgggt atcaatccta cgcttacgct gataagtttg
    37921 ctggcaggat tggcaacgat agagcttgat tggcttggag ttactattgg gcaaggatgg
    37981 tacaaaccgt aatccatcca ctgctttcaa cagttcctta aaatcccgat ccttgtgttg
    38041 atagcctttc ccttgaaaat agaggtgata atgacagagt tcatgtcgga caattttcct
    38101 aaaaacgtcc aaccccagtt cctgataaac cttgggatta aaatccaaat gcccatcttt
    38161 ggggaaaaat cgcccacctg tcgaacgtag acgcctattc cactggacat gatggataaa
    38221 aggtctgccg aagtcttcta gtgaaacctg cttgacgtaa tcagtcagtt tcatttggag
    38281 ctaggagaga cagattaact ttttcacgtt cagtatcaat tttcttaacc caaacgctca
    38341 ccaaatctcc aactgccacc acttgactag ggtgtttgat aaacttgcga ctcatatggg
    38401 aaatatggat gagaccgtcc tcatgaattc cgatatcaac aaaagcaccg aaatcaacaa
    38461 cattacgcac cactccttct agcttttgtc caaccactaa gtccttgata tctaggacat
    38521 cttgacggag aactggagca tcaaaggaat cacggaaatc tcgacctggt ttgagaagat
    38581 ctgcaatgat atctttaaga gtttctggac caaggtctaa ctcttgcgcc atttccttga
    38641 ctgaaagcga cttgagttta ctttgggcct cttcattcag atccttaatg tccaagcatt
    38701 tgaagagttc cttaacggca gcgtaatttt ctgggtgaac tcctgtatta tcaaggatat
    38761 tgctactttc agggatacgg aggaaaccag cagcctgttc aaaagccttg gcccccaaac
    38821 gaggaacttt cttgatttgg gagcgtgaag tgatttttcc ctcttcctca cggtatttga
    38881 cgatattttc agaaatggtt ttattgagtc cagctacgtg tgaaagaaga gctgggctag
    38941 ctgtattgac attgacacca acttggttaa ccactgtatc gacaacaaag tccagactct
    39001 cagatagttt cttctgactg acatcgtgtt ggtattgacc gacaccaatt gacttaggat
    39061 cgattttgac caattccgca agaggatctt gcaaacgacg ggcgatagaa atggcagagc
    39121 gtttttcaac ggtcaagtct ggaaactcct gacgagcaag ttcgctggca gaatagacag
    39181 aagcaccact ttcattaacg ataacatagc tgacttcagg gaaatctttc agaacttccg
    39241 ctacaaaagc ttcactttca cgactggccg ttccatttcc aatggcaata atctctacac
    39301 cgtattgacc aattaaatct gctaaatctt tcttggcttc ttcgatttga cgagctgatg
    39361 ctggtttaac aggataaata acctgagttg tcagcatttt tcctgttgca tccacgacag
    39421 ctagcttggc acctgtacga aaggctgggt caaacccaag aaccacgcgc cctttcagtg
    39481 gagcaaccaa gaggagattg cgcagattgt cagaaaaaag ttggatagct ccctcttcag
    39541 ctttctcagt taattctgtc cgaatacgac gctcaatagc aggcaagacc tttttcttaa
    39601 cggattgctg aacaacttca tcaatataag catttttcac cttgaaacga gtagcaaaga
    39661 aggcaagaat acggtccgtc gcatgttcaa aaccgatctt caagacacca agtttctccc
    39721 cacgattgag agccaaggta cgatagcctt gcatagttcc aactgtctct gaaaaatcat
    39781 aataaatctg aaaaacctgc ttttcatcaa gactttcatc cttggcttga gaagtgagtt
    39841 tagagtgtct cagcacttcc tgataagtca tagaacgcaa ggtcacatct tccgataagg
    39901 cttcgaccaa aatatcaact gcaccggtca aggcttcctt gccagtcgca aatccttcac
    39961 agacgaactt ttcagcttct ttctctaagt caactatatt ctgcaaaatc aaacgagcaa
    40021 gaggaaagag tccagcttca cgggcaatgg ttgccttggt acgacgcttt tccttataag
    40081 gaagatagag ttcttcaacg tctgctaatt tttcggcaac taagatagct tcttccaatt
    40141 ccttggtcaa cttaccttgt tcttgaatct tagctaagac agcttcctta cggtcattga
    40201 gatttgtcag acttttatcc aaatcaataa tagccttaat cgccacctca tccagactac
    40261 cagtcatgtc cttgcgataa cgcgcgataa agggaatagt cgccccttca gctgtcaaac
    40321 ttagaacggt atcaatttgc tttaacgtca ctcccaaatc ctgagagatt ttttcatatt
    40381 ttttatccat aaatctatta taccacaagc taaacgtttc aaattaactc gtagaacatt
    40441 taaaaaatat gtaggaaata gatttatatg ctacagcgca ataacttgca cttaaagagc
    40501 attgccacct ttttttaacc aagccatgat atcaaaagta tttaatggat cagacataat
    40561 agccagttct ggaagatgtt cctgacctgg aataacacat tgacttttca aatttttata
    40621 tggacgattg actaaaatta atttattaga ataaggaaga ttatccatct tatttaaaat
    40681 ttcttcacta gctgaatctt tattatcaaa tttaaaataa agattattcc aatttatgcg
    40741 tttttttctt ttttcccact tagttcgtgc ttcttcaata ctagaataat gtagaaaatg
    40801 aatatctata tctcctaagt gccccaaagg ataaacttca tgagtccagc tcggtgaaat
    40861 aagttcctct tcgaaaacaa gttcttgttc catataataa cgaaaatgct ttgtaagttt
    40921 ataataatca tcaggaagaa taaataaacc aacaaaaggt gttctatatt gaaaaccaag
    40981 ctgtttataa attaatcctc c
//
LOCUS       DAVKQB010000014        40075 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKQB010000014 DAVKQB010000000
VERSION     DAVKQB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.014"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            139..897
                     /locus_tag="VW528_000729"
     CDS             139..897
                     /locus_tag="VW528_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9911579.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            906..1709
                     /locus_tag="VW528_000730"
     CDS             906..1709
                     /locus_tag="VW528_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9911580.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            1731..2990
                     /gene="rseP"
                     /locus_tag="VW528_000731"
     CDS             1731..2990
                     /gene="rseP"
                     /locus_tag="VW528_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9911581.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPKDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            3003..4856
                     /locus_tag="VW528_000732"
     CDS             3003..4856
                     /locus_tag="VW528_000732"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9911582.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTSEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            4955..6334
                     /gene="bglA"
                     /locus_tag="VW528_000733"
     CDS             4955..6334
                     /gene="bglA"
                     /locus_tag="VW528_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9911583.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEHYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            6527..8335
                     /gene="glmS"
                     /locus_tag="VW528_000734"
     CDS             6527..8335
                     /gene="glmS"
                     /locus_tag="VW528_000734"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9911584.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLSLLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGFDVDKPRNLAKSVTVE"
     gene            complement(8473..8550)
                     /locus_tag="VW528_000735"
                     /pseudo
     CDS             complement(8473..8550)
                     /locus_tag="VW528_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            8607..9656
                     /locus_tag="VW528_000736"
     CDS             8607..9656
                     /locus_tag="VW528_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9911585.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLALLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(9756..13592)
                     /gene="spuA"
                     /locus_tag="VW528_000737"
     CDS             complement(9756..13592)
                     /gene="spuA"
                     /locus_tag="VW528_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9911586.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNKSDISSLGNANASLEKTEEKPATEPT
                     TPAASPADPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQ
                     GLWTWDDVEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNL
                     TGDKSVEKLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVK
                     NPSSAQWPDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFT
                     DLKNHSQIFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKH
                     SNITNHLGNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDE
                     TYSYDGKLGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTW
                     KQTLDSTNKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSNDAKIDDAHKVA
                     KAAFVDPAKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEA
                     FIEKLDYLKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSL
                     TGMYSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFM
                     DADGTPRTSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEA
                     YKAARALNPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGY
                     PNEGQPAFITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSI
                     KKDPSKAENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDK
                     VPNKSHLLRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKG
                     LIALRQSTDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVN
                     ADEKAREFNLGTAFAHLRNAEVLADENQAGPVGIANPKGLEWTEKGLKLNALTATVLR
                     VSQNGTSHESTAEEKPDSTPSKPEHQDPAPEARPDSTKPDAKVADAENKPSQATADSQ
                     AEQPAQEAQASSVKEAVQNESVENSSKKNIPATPDRQAELPNTGIKNENKLLFAGISL
                     LALLGLGFLLKNKKEN"
     gene            14009..14422
                     /gene="rpsL"
                     /locus_tag="VW528_000738"
     CDS             14009..14422
                     /gene="rpsL"
                     /locus_tag="VW528_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9911587.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            14442..14912
                     /gene="rpsG"
                     /locus_tag="VW528_000739"
     CDS             14442..14912
                     /gene="rpsG"
                     /locus_tag="VW528_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9911588.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            15337..17418
                     /gene="fusA"
                     /locus_tag="VW528_000740"
     CDS             15337..17418
                     /gene="fusA"
                     /locus_tag="VW528_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9911589.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            17522..21913
                     /locus_tag="VW528_000741"
     CDS             17522..21913
                     /locus_tag="VW528_000741"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9911590.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREVFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            22015..22278
                     /locus_tag="VW528_000742"
     CDS             22015..22278
                     /locus_tag="VW528_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9911591.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            22271..22549
                     /locus_tag="VW528_000743"
     CDS             22271..22549
                     /locus_tag="VW528_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9911592.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            22570..22722
                     /locus_tag="VW528_000744"
     CDS             22570..22722
                     /locus_tag="VW528_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911593.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALREK"
     gene            22744..23985
                     /locus_tag="VW528_000745"
     CDS             22744..23985
                     /locus_tag="VW528_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9911594.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            23995..24225
                     /locus_tag="VW528_000746"
     CDS             23995..24225
                     /locus_tag="VW528_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9911595.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            24448..25170
                     /locus_tag="VW528_000747"
     CDS             24448..25170
                     /locus_tag="VW528_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9911596.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            25387..26721
                     /gene="pepC"
                     /locus_tag="VW528_000748"
     CDS             25387..26721
                     /gene="pepC"
                     /locus_tag="VW528_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9911597.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQTKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(26777..27688)
                     /locus_tag="VW528_000749"
     CDS             complement(26777..27688)
                     /locus_tag="VW528_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9911598.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(27712..28515)
                     /locus_tag="VW528_000750"
     CDS             complement(27712..28515)
                     /locus_tag="VW528_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9911599.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(28543..29532)
                     /gene="manX"
                     /locus_tag="VW528_000751"
     CDS             complement(28543..29532)
                     /gene="manX"
                     /locus_tag="VW528_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9911600.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(29765..30784)
                     /gene="adhP"
                     /locus_tag="VW528_000752"
     CDS             complement(29765..30784)
                     /gene="adhP"
                     /locus_tag="VW528_000752"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9911601.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSETMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(31123..31935)
                     /locus_tag="VW528_000753"
     CDS             complement(31123..31935)
                     /locus_tag="VW528_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9911602.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            32125..33543
                     /locus_tag="VW528_000754"
     CDS             32125..33543
                     /locus_tag="VW528_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9911603.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTKVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            33596..34303
                     /locus_tag="VW528_000755"
     CDS             33596..34303
                     /locus_tag="VW528_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9911604.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLMVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            34428..35372
                     /gene="folP"
                     /locus_tag="VW528_000756"
     CDS             34428..35372
                     /gene="folP"
                     /locus_tag="VW528_000756"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9911605.1"
                     /translation="MSSKANHAKTAICGIINVTPDSFSDGGQFFALEQALQQARKLIA
                     EGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDTWKSQVAEAAL
                     AAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLIFPHFGFGQTF
                     TEKELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGLTKKENLLLLR
                     DLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTASAHVTSIAARQ
                     GVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            35374..36696
                     /locus_tag="VW528_000757"
     CDS             35374..36696
                     /locus_tag="VW528_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9911606.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLESIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEEKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGCFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADH
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKARNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            36677..37231
                     /gene="folE"
                     /locus_tag="VW528_000758"
     CDS             36677..37231
                     /gene="folE"
                     /locus_tag="VW528_000758"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9911607.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            37274..38086
                     /gene="folK"
                     /locus_tag="VW528_000759"
     CDS             37274..38086
                     /gene="folK"
                     /locus_tag="VW528_000759"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9911608.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(38237..38554)
                     /locus_tag="VW528_000760"
     CDS             complement(38237..38554)
                     /locus_tag="VW528_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9911609.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            38911..39357
                     /gene="rplM"
                     /locus_tag="VW528_000761"
     CDS             38911..39357
                     /gene="rplM"
                     /locus_tag="VW528_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9911610.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISELI"
     gene            39376..39768
                     /gene="rpsI"
                     /locus_tag="VW528_000762"
     CDS             39376..39768
                     /gene="rpsI"
                     /locus_tag="VW528_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9911611.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
BASE COUNT        11394 a         7773 c         9284 g        11624 t
ORIGIN      
        1 aaaaataaag tcgcccaatc aggcggctta tttttttgaa aaatgggctt ggtgcctgag
       61 aataaatagc ttagtgatag aagaaaatgg ggaaatatgg tataataaaa cgatagattt
      121 ttgaatagga ataagatcat gtttggattt tttaagaaag ataaggctgt ggaagtagag
      181 gttccgacac aggttcctgc tcatatcggc atcatcatgg atggcaatgg ccgttgggct
      241 aaaaaacgta tgcaaccgcg agtttttgga cataaggcgg gcatggaagc attgcaaacc
      301 gtgaccaagg cagccaacaa actgggcgtc aaggttatta cggtctatgc tttttctacg
      361 gaaaactgga cccgtccaga tcaggaagtc aagtttatca tgaacttgcc agtagagttt
      421 tatgataatt atgtcccgga actacatgcg aataatgtta agattcaaat gattggggag
      481 acagaccgcc tgcctaagca aacctttgaa gctttaacca aggctgagga attgactaag
      541 aacaacacag gattgattct taattttgct cttaactatg gtggacgtgc tgagattaca
      601 caggcgctta agttgatttc ccaggatgtt ttagatgcca aaatcaatcc aggtgacatc
      661 acagaggaat tgattggtaa ctatctcttt acccagcatt tgcctaagga cttacgagac
      721 ccagacttga ttatccgtac tagtggagaa ttgcgtttga gcaatttcct tccatggcag
      781 ggagcctata gtgagcttta ttttacggac accttatggc ctgattttga cgaagcggcc
      841 ttgcaggaag ctattcttgc ctataatcgt cgtcatcgcc gatttggagg agtttaggag
      901 gaaatatgac ccaggattta cagaaaagaa ccttgtttgc agggattgcc ctggctattt
      961 tcctaccaat tttaatgatt gggggcctct tgcttcagat agcaatcgga atcatagcca
     1021 tgctagccat gcatgaactt ttgaagatga gaggtctaga gaccatgacg atggagggcc
     1081 tcttgaccct ctttgcaacc tttgcattga ccattccctt ggagaattac ctgacttttt
     1141 tgccagttga tgggaatgtg gttgcctata gtgttttgat ttcaatcatg ttaggaacga
     1201 ccgtttttag caagtcttat acgattgagg atgcggtttt ccctcttgct atgagcttct
     1261 acgtgggctt tggatttaat gctttactag atgctcgtgt tgcaggtttg gacaaggctc
     1321 tcttagcctt gtgtatcgtc tgggcgacag acagtggtgc ctatcttgtt gggatgaact
     1381 atgggaaacg aaagttagca ccaagggtat cgcctaataa aacccttgag ggtgccttgg
     1441 gtggtatttt aggagcaatt ttagtaacca ttatctttat gatagttgac agtacagttg
     1501 ctcttccata tggaatttac aagatgtcag tctttgctat tttctttagc attgctggac
     1561 aatttggtga tttactagaa agttcgatca aacgtcattt tggtgttaag gattctggga
     1621 aatttatccc tggacatggt ggtgttttgg atcgtttcga tagtatgttg cttgtatttc
     1681 caatcatgca cttatttgga ctcttttaat caaaagacgg aggaaacgct atgctcggaa
     1741 ttttaacctt tattctggtt ttcgggatta ttgtagtggt gcacgagttc gggcacttct
     1801 actttgccaa gaaatcaggg attttagtac gtgaatttgc catcggtatg ggacctaaaa
     1861 tttttgctca cattggcaag gatggaacgg cctataccat tcgaatcttg cctctgggtg
     1921 gctatgtccg catggccggt tggggtgatg atacaactga aatcaagaca ggaacgcctg
     1981 ttagtttgac acttgctgat gatggtaagg ttaaacgcat caatctctca ggtaaaaaat
     2041 tggatcaaac agccctccct atgcaggtga cccagtttga ttttgaagac aagctcttta
     2101 tcaaaggatt ggttctggaa gaagaaaaaa catttgcagt ggatcacgat gcaacggttg
     2161 tggaagcaga tggtactgag gttcggattg cacctttaga tgttcaatat caaaatgcga
     2221 ctatctgggg caaactgatt accaattttg caggtcctat gaacaatttt atcttaggtg
     2281 tcgttgtttt ttgggtttta atctttatgc agggtggtgt cagagatgtt gataccaatc
     2341 agttccatat catgccccaa ggtgccttgg ccaaggtagg agtaccagaa acggcacaaa
     2401 ttaccaagat tggctcacat gaggttagca actgggaaag cttgatccaa gctgtggaaa
     2461 cagaaaccaa agataagacg gcaccgactt tggatgtgac tatttctgaa aaggggagtg
     2521 acaaacaagt cactgttaca cccaaagata gtcaaggtcg ttaccttcta ggtgttcaac
     2581 cgggggttaa gtcagatttt ctatccatgt ttgtaggtgg ttttacaact gctgctgact
     2641 cagctctccg aattctctca gctctgaaaa atctgatttt ccaaccagat ttgaacaagt
     2701 tgggtggacc tgttgctatc tttaaggcaa gtagtgatgc tgctaaaaat ggaattgaaa
     2761 atatcttgta cttcttggca atgatttcca tcaatattgg gatttttaat cttattccga
     2821 ttccagcctt ggatggtggt aagattgtgc tcaatatcct agaagccatc cgccgcaaac
     2881 cattgaaaca agaaattgaa acctatgtca ccttggccgg agtggtcatc atggttgtct
     2941 tgatgattgc tgtgacttgg aatgacatta tgcgactctt ttttagataa tcgaggaata
     3001 ttatgaaaca aagtaaaatg cctatcccaa cgcttcgcga aatgccaagc gatgctcaag
     3061 ttatcagcca tgctcttatg ttgcgtgctg gttatgttcg ccaagtttca gcaggtgttt
     3121 attcttatct accacttgcc aatcgtgtga ttgaaaaagc taaaaacatc atgcgccaag
     3181 aattcgaaaa gattggtgct gttgagatgt tggctccagc ccttcttagt gcagaattgt
     3241 ggcgtgaatc aggtcgttac gaaacctatg gtgaagacct ttacaaacta aaaaaccgtg
     3301 aaaaatcaga ctttatctta ggtccaactc acgaagaaac ctttacagct attgtccgtg
     3361 attctgttaa atcttacaag caattgccac tcaaccttta tcaaattcag cccaagtatc
     3421 gtgatgaaaa acgcccacgt aatggacttc ttcgtacacg tgagtttatc atgaaggatg
     3481 cttatagttt ccacgctaac tatgatagtt tggatagtgt ttatgatgag tacaaagcag
     3541 cctatgagcg tatttttact cgtagtggtt tagacttcaa ggctattatt ggtgacggtg
     3601 gagccatggg tggtaaggat agccaagaat ttatggccat tacatctgct cgtacagacc
     3661 ttgaccgctg ggttgtcttg gacaagtcag ttgcctcatt tgacgaaatt cctgcagaag
     3721 tgcaagaaga aatcaaggca gaattgctca aatggatagt ctctggtgaa gataccattg
     3781 cttactcaag tgagtctagc tatgcagcta acttagaaat ggcaacaaac gagtacaaac
     3841 caagcaaccg tgttgtcgct gaagaagaag ttactcgtgt tgcaacgcca gatgttaaat
     3901 caatagatga agttgcagct ttcctcaatg ttccagaaga acaaacgatt aaaaccctct
     3961 tctacattgc agatggtgag cttgttgcag cccttctagt tggaaatgac caactcaacg
     4021 aagtcaagtt gaaaaaccac ttaggagcag atttctttga cgttgctagc gaagaagaag
     4081 tggcgaatgt tgttcaagca ggatttggtt cacttggacc agttggtttg ccagagaata
     4141 ttaaaattat tgcagatcgt aaggtgcaag atgttcgcaa tgcagttgtc ggtgctaacg
     4201 aagatggcta ccacttgact ggtgtgaacc caggccgtga ttttacttca gaatatgtgg
     4261 atatccgtga agttcgtgag ggtgaaattt ccccagatgg acaaggtgtc cttaattttg
     4321 cgcgtggtat tgagatcggt catattttca aactcggaac tcgctattca gcaagcatgg
     4381 gagcagatgt cttggatgaa aatggtcgtg ctgtgccaat catcatggga tgttacggta
     4441 tcggtgtcag ccgtcttctt tcagcagtga tggagcaaca cgctcgcctc tttgttaaca
     4501 aaacgccaaa aggtgaatac cgttacgctt ggggaatcaa tttccctaaa gaattggcac
     4561 catttgatgt gcatttgatt actgttaatg tcaaggatga agaagcgcaa gccttgacag
     4621 aaaaacttga agcaagcttg atgggagctg gttatgaagt cttgacagat gaccgtaacg
     4681 aacgtgtcgg tgttaaattt agcgatagtg acttgattgg tcttccaatc cgtatcactg
     4741 ttggtaaaaa agcggccgat ggcattgtag aagttaagat caaggcaact ggtgacacca
     4801 ttgaggttca tgcagataac gtgcttgaaa cgcttgaaat cctaagtaag aaataaaaac
     4861 tataatcaga agaaaaacaa ggaaaaaatg taactagttt ttaccttgtt ttttctgtat
     4921 aatgggagaa atgagtagat aaagaggtaa atgtatgcta agatttccaa aggattttgt
     4981 ctggggatcc tctacttctg gaccgcagac agaaggacgt gtagctggtg acggtaaggg
     5041 agacaatctc tgggattact ggttccaagt ggagccaaat cgttactata atgggattgg
     5101 ttcagataag acatcgactt tttatgaaaa ttgggagcgg gatattgagc ttttgttaga
     5161 gactggtcac acagcctttc ggacttctat tcagtggtca cggatttttc cacaaggctg
     5221 tggaaaagtc aaccctcaag gtgtggattt ttatcgtaag gtctttgagg ctattaaggc
     5281 taaaggaatt cgtctgttag tcaatctcta tcattttgat ttaccttttg cccttcaaga
     5341 ggatggtgat ggttgggaaa ataaggcgac agtttcagcc tatgaagact atgctcgttt
     5401 ttgttttgag acttatggag atttagtgga tcaatggatt acctttaacg agcccatcgt
     5461 tcctgtagaa tttggttatt tttatgatgc ccattatcca cataaggtgg atgcagaggc
     5521 agctgttaag gtagcctatc atacacaatt ggccagcagc cgggctgtta aggcttgcca
     5581 tgaacttttg cctgattcca agattgggat tgtcctcaac ttgacaccgg cttatccacg
     5641 tagccagcat cctgctgatg tcaaggcagc tcgtattgcg gccctttttc aggcccaatc
     5701 tttcttagat ccatctgtct tggggactta tccacaggag ttggtagaaa tcttgcatga
     5761 acacggtctt ttacctgatg ctacagagga agagttggaa ctcattcgtg ataatacggt
     5821 ggacttcctt ggtgtgaact actatcaacc tttgcgtgtt atggctcctc gatttgctaa
     5881 gcatccagag agtccactct taccagaaca tttttacgag cattatgtga tgcctggacg
     5941 taaaatcaat tctcaccgtg gttgggagat ttatgagcaa gggatttatg acatcgccca
     6001 aaatatcaag gaaaattatg gcaatattga gtggatgttg actgagaatg gtatgggtgt
     6061 tgaaggggaa gaaaaattcc gtcaagatgg aatgattcaa gatgattacc gtattgactt
     6121 tgtaaaaggt catcttcgtg aacttcaccg tgccattgaa gatggtgcca actgtaaggg
     6181 ctacttgatt tggaccttta ttgactgctg gtcatggctc aacagctata aaaatcgcta
     6241 tggtttggtc gaattagact tggaaacgca agaacgtcgt ctgaagaaat caggccactg
     6301 gttcaaagaa ttaagcgata ataatggatt ttaaaaagga ctctgactga ttatcccaat
     6361 cggtcagagt ttttatgctt acaggagctt aatttgaact ataccaattt ttactcttga
     6421 caagtgaaag aaacaagtat atactgtttt tactgattct ataaaagatg aattagacta
     6481 taccaatttt aaggagaaag cacagcttgc ctgtgtcgta tatactatgt gtggaattgt
     6541 tggtgttgtt ggaaacacaa atgcaactga tattttgatt caagggcttg aaaagcttga
     6601 ataccgtggc tatgattctg cgggaatttt tgtcctagat ggtgctgata accatttggt
     6661 gaaggcggtt ggtcgtattg cagaattgtc tgccaagaca gctggtgttg agggaacaac
     6721 tggtatcgga catactcgtt gggctactca cggaaaacca actgaggaca atgctcaccc
     6781 acaccgctct gagacagaac gttttgtctt ggtgcataat ggggtgattg agaactatct
     6841 tgaaatcaag gaagaatacc ttgcaggtca ccacttcaag gggcagacag atactgaaat
     6901 agccgttcat ttgattggaa aatttgcgga agaagaaggg ctctcagttc ttgaagcctt
     6961 caaaaaagct cttcatatta tccgtggttc atatgccttt gccttgattg actctgaaaa
     7021 tccagatgtc atctatgtag cgaaaaacaa atctccactt ttgattggtc ttggggaagg
     7081 ctacaatatg gtctgctcag atgctatggc tatgattcgt gaaaccaacc aatacatgga
     7141 aattcatgac caagagttgg taatcgtcaa ggctgatagc gtggaagttc aagactatga
     7201 tggtaacagt cgtgaacgtg ctagctatac tgcggaactt gacttgtcag atatcggtaa
     7261 gggaacttat ccttactaca tgcttaagga aattgatgag caaccaactg ttatgcgtaa
     7321 actcattcaa gcctacacgg atgatgctgg tcaagtagtg attgatcctg ctatcattaa
     7381 ggctgttcaa gacgcagacc gcatctacat ccttgcagct ggaacatctt accatgcagg
     7441 atttgcttct aagaaaatgt tggaagaatt gacagataca ccagttgaac ttggaatctc
     7501 atctgagtgg ggctacggta tgccacttct cagcaagaaa ccactcttca tctttatcag
     7561 ccaatctggt gaaacagcgg atagtcgtca agttttggtc aaggctaatg aaatgggaat
     7621 tccaagctta acagtgacaa acgttccagg ttcaaccctc tcacgtgaag ccaactatac
     7681 catgctcctt cacgcaggtc ctgaaattgc cgtggcatca actaaagcct atacagcgca
     7741 aatcgcagcc cttgccttcc ttgcaaaagc agtcggagaa gcaaatggta atgctaaagc
     7801 gcaagccttt gacctggttc atgaattgtc aatcgtagct cagtctatcg aatcaactct
     7861 ttcagagaaa gaaaccattg aagccaaggt tcgtgaactt cttgaaacaa ctcgtaacgc
     7921 cttttacatc ggacgtggtc aagattacta cgtagccatg gaagcaagtc tcaaactcaa
     7981 agagatttct tatatccagt gtgaaggttt tgcggcagga gaactcaagc acggaaccat
     8041 tgccttgatt gaagaaggaa cgcctgtctt gtctctcttg tcagatccag ttcttgccaa
     8101 ccatactcgt ggaaatatcc aagaggtcgc agcccgtggt gccaaagtcc tcactatcgc
     8161 agaagagaat gtagccaaag ataccgacga tatcgtcctt acgaccgtac atccatacct
     8221 ctcaccaatt tcaatggtcg taccaacgca attagtcgct tactttgcaa ccctccaccg
     8281 tggcttcgat gtggacaaac cacgtaacct tgccaagtca gtaacggtag aataagctaa
     8341 aaaagtctag ttatctaggc ctttttcttg tgagcaaatt ggttgcttgt actcaagatt
     8401 cgtgttatac tcttcgaaaa tctcttcaaa ccacgtcaac gtcgccttgc cgtaggtatg
     8461 gttactgact tcgtcagttc tatccacaac ctcaaaacac tgttttgagc agcctgcagc
     8521 tagcttccta gtttgctctt tgattttcat tgagtattag aataattttt caaattgaag
     8581 tacagagata gataaggaga atcacaatgg tagaattggg aatttcaaca tttggggaaa
     8641 taacggagct tgaagggact ggacaaactt acagtcatgc tgaacgcatt cgccagttgg
     8701 tggcagagat tgagctggct gacaaggttg gtttggatgt atatgggatt ggtgagcacc
     8761 atcgggcgga ttttgcagta tcagccccag agattgttct ggccgctggg gcagtcaata
     8821 ccaagaaaat ccgtttgacc agtgcagtca gcattctgtc aagcatggac ccgattcgtt
     8881 tgttccaaca gtatgccact atcgatgctt tgtcaaatgg acgagcggag attatggctg
     8941 gaaggggctc tttcacggaa tctttccctc tgtttggtta tgacttgaaa gactacgact
     9001 ctctttttga tgagaaatta gccttgcttc agttagtcaa tgaaaagacc aagttagact
     9061 ggcaaggtcg attgactcaa acgattgctg gtaaagaagt ttatcctcgt ccagttcagg
     9121 acaaattgcc cttgtggata gctacaggtg gtcatgtcga atcaacagtg aagattgctc
     9181 aggctggtct accgattgta tatgctatta ttggtggtaa tccacgttat tttaaaaagt
     9241 tgattcaggc ttatcgtgag attggaagcg aagcgggtca tgctgacaaa gacttgaaag
     9301 tgggagccca ttcttggggg tggattgcgg aagatggtga gcaggcggtg aaagattatt
     9361 tccatccgac taagcaagtg gtggatgcta tttccaaaga ccgtccgcac tggcaggaat
     9421 tgcgttatga gcaatatttg gagcaggtag ggccaaatgg cgccatgttt gttggcaatc
     9481 cagatcaggt ggccgaaaaa ttgattcgca tgatagagga tttaggattg gaccgcttca
     9541 tgcttcatct accgcttggt tctatgcctc atgaccaggt tctgagagct attgaactct
     9601 tcggaacaca agttgcaccc aaagtacgag cttattttgc tatgaaagag gcttaataaa
     9661 aaatcctaat caagtttatt aggacaacaa atattgtacg aaaattaatc ccccgagcta
     9721 taatgcttgg gggatttttc tataggaggg ctagtttagt tctctttttt attttttagt
     9781 aagaaaccga gaccaaggag cgcaaggagg ctgattcctg caaataggag tttgttttcg
     9841 tttttgattc ctgtatttgg aagttcagct tgtctatctg gggttgcagg tatatttttc
     9901 ttgctagagt tttctaccga ttcgttttga accgcttctt ttacagatga tgcttgtgct
     9961 tcttgtgctg gttgttcagc ttgtgaatca gctgtagctt ggctaggttt attttccgca
    10021 tcagctactt tggcatctgg tttagtagaa tcaggtctag cttctggagc tgggtcttga
    10081 tgttcaggct tggaaggggt tgagtctggt ttctcttctg cagttgactc atggctagtt
    10141 ccattttgag agactcgaag aacagtagct gtaagggcat tcaatttcaa gcctttttca
    10201 gtccattcaa gtcctttcgg gttggcaatt ccgactggtc ctgcttggtt ttcatctgcc
    10261 aaaacttccg catttcttag atgtgcaaag gcagttccca aattaaattc gcgagctttt
    10321 tcatccgcat tgacaaagac tgcgtagata tcgccgtttg gagcagtgat ttggtagcca
    10381 atcactacat cctctttttc cacaccattt tggcctggga cagtgatgag gtggacacgg
    10441 tctttgatat cttggagact cttaagtcgg aaggcatctg tagattgacg aagggcaatc
    10501 aaacctttca tatagtcacg gctcttgaca ttttcaggat aagctttacc atctgtagcc
    10561 ttagtccagt caaacttgtt gactgcgtcg ctagaatcgt aagaatcatg gatgaagtaa
    10621 ggatagtcaa atgggttgcc gtccttatca cgcaacaagt gagatttgtt tggaacctta
    10681 tcctctgcta ctggagtctt gtaggctggg tcacggaatt gtttagtacg tccatattcc
    10741 tgaccggagt ggataaatgg agttccttga gctgtcaaga ccatgagatt tccaagtcgt
    10801 aaacgacggt ggatttcagc atagttctca gccttgcttg ggtctttttt gatagactgg
    10861 gcaatgatgt caaagagggt caagttatca tgggctgcga tgtattggat gacatctcca
    10921 gggctgtcag cttcaaagtt agttggttga gcaatgagat ttttaaagat ggtgttgaca
    10981 tcacgcttgc cacctgtgat aaaggcaggt tgaccttcgt ttggataacc agatttgagg
    11041 ttgttacgga tgtcatctga aaagacagcg acagtatcgg tatgtttcat ccaatcttgg
    11101 tcagcagctt tagtaggcat gttttcatca ccggcatagg ttctccaacc ttcgcctaac
    11161 atgattagat ttggattgag ggcgcgtgca gccttgtaag cttcttcgat agaagcggcg
    11221 tcatggtctc ccatcatatc gaagcggaag ccatccactt tgtaggtatc aactaggtat
    11281 ttgatagagt caactaggag gcgtttggtc atatggtggg ttgtccccaa gcgtccacca
    11341 ccaaagctag ttcgaggtgt gccatcggca tccataaagt ggtagtagtt tggctctagg
    11401 tcttcaaaga tatcgacttt ggctgtgtgg ttataaacga catctaggat agctcccata
    11461 ccacgtttgt ggatttcgtt gatgaggttt ttaaattctg cgattcgttt ttctggattc
    11521 ttaggatcgc ttgagtacat accagtcaag gagaagtagt tttgagggtc atatccccag
    11581 ttgtagttgc tgttgcttga agcatagtca gacaagcgtt catggttttt caattcattg
    11641 acaaagtagt aagacaagac tggaaggagc tggatatggg ttacacccaa gtctttgaga
    11701 tagtctagtt tttcaatgaa ggcttcaaaa gtcccaaatg gtttggtcaa gtcttttgca
    11761 atggcaggat ctgaagtgaa gtcacgcaca tgagcttcgt agataacggc gtcttcacga
    11821 gtcttgaaat tgtgaatctt accataagtc aagtcttgag gtccgagttt agctggatct
    11881 acaaaggcgg ctttagccac tttatgggca tcgtcaatct tggcatcgtt gctattccaa
    11941 gcagcaagag atttagcgta aggatcgagt gcaagaacag ttttaccttg acgctcgatt
    12001 tggtattgat aatagtagcc agtgaaatct gtgattccga gtttgtttgt gctgtctaga
    12061 gtttgtttcc aagttcctct ttcccctttt tcgagagcga cagttccaac tactttgtca
    12121 gggtcattct tgtcgtagac aacaacagaa accttatcag cacttggtga ccaaagggtc
    12181 aaatcaactt gttttccttc ttcttttagg tcagctccca gtttgccatc atagctgtat
    12241 gtctcatctt tcaggcgcca gcttgttttg gtagtgaatt ggtcggaatt gtagctaaca
    12301 gtataaggat gttttgtgtc agagaaatct ccgctgtagg tcactttctt accagcttca
    12361 tcgattgcaa catcggtaat agttaccttg tttcctaggt gattagtgat gttggagtgt
    12421 ttgaggatat cttctttttt agcaccgaca agtgttgaaa agctactttc aatgctagaa
    12481 gtgcctacgt gttgggctcc tgtcatacgg atatcatgga catagtatgg atttgtgtaa
    12541 atcgattcat catcgtcttt taggaaaatt tggctatgat ttttcaaatc tgtgaactta
    12601 taattttctt tacggatttt cacgtcgtct ccttgtttgc tctcatctag taataaaaat
    12661 ccaaattctc ttgcggcttc attaagagga atgtcgatat agcggccata tttgcctgta
    12721 gccgtaaagt ctgttccgtc aggccattga gcgctacttg gatttttcac atctccccag
    12781 taccagagag atttcttgtc atagttgcca tctgtgcggt agtagttgac gcgaacagtt
    12841 cctgcaggct gtggctcgta agagaaaacc ttgtaatctt ggtctaacca agcttcgttc
    12901 atttttggaa ctagtttttc tacagattta tcgccggtta gattttttcc agctgtattg
    12961 ttgatgagga agctaatttt cttggcttgt tctcccttta atttgacatc taggtaatag
    13021 ccgtagtcat ctttcttggc atccttgaag gacaaagctc cgtttggcca gttttcagat
    13081 ggtttttcaa catcgtccca agtccatagt ccttgagcat ccttgttttc ttcaggaagt
    13141 tttttgacat ggatacggaa gtagttatct tcgatgggct cttctgcctt agtttcagtt
    13201 gttactggac tagtagaagt agttggctca cttgaacgat cttgtccagt ttgtggtgct
    13261 ggatcggctg ggcttgcagc aggagttgtt ggctctgttg caggtttttc ttctgttttc
    13321 tctagggaag cgtttgcatt tccaagtgaa gaaatatcac ttttgttctt accagagcca
    13381 gctgctgtat cagtaagagg ttgggcaagg gtggttgtat ttctgttgtt gggttcgtta
    13441 gtggttggtg tactttcgtc ggatgagata gttggtgtag ccatggcaag caagacaagg
    13501 cttgcgccga taaggacaga accagtccca tttttgaggg aacggatgct gtagaccatt
    13561 tttttctcag tgtgagatgg tgtttttctc ataatgattc tccaatcaat aaatttctat
    13621 atcagtatag catgtagtaa aacaatatgc aagcgttttc tttgatttgt gaaaaaaatt
    13681 cttacttttg agaagttttc tgtactattt tatatatcag tataaatgac ggagataaaa
    13741 atgtcacata agccaagccg tattattagg aagatgatgt gttcccagct atttttattc
    13801 agaaattgat ctctaacatt accaataaaa aagaaaaata tcttgacaaa caagggaaaa
    13861 ttttgttaca ataatagacg gtacttttta cttttggtct ctcaagagtg tacagggacg
    13921 tgctgacaaa tgttgcaaaa gtacacacag atgatatctg tcaccaagtg tatcatcacc
    13981 aaaaataaaa aaacacagga gaatgtagat gcctacaatt aaccaattgg ttcgcaaacc
    14041 gcgtaaatca aaagtagaaa aatctaaatc accagctttg aacgttggtt acaatagtca
    14101 taaaaaagtt caaacaaacg tttcttcacc acaaaaacgt ggtgttgcaa ctcgtgttgg
    14161 aacaatgaca cctaaaaaac ctaactcagc ccttcgtaaa ttcgctcgtg tacgtttgag
    14221 caaccttatc gaagttactg cctacatccc aggtatcgga cacaacttgc aagagcacag
    14281 cgtggtgctt cttcgcggtg gacgtgtaaa agaccttcca ggggtacgtt accatatcgt
    14341 ccgtggtgca cttgatactg caggtgttaa cgatcgtaaa caaggtcgtt ctaaatacgg
    14401 tactaaacgt ccaaaagcat aaggaaaggg gataaagaga aatgagtcgt aaaaatagag
    14461 ctccaaaacg tgacgtattg ccagatccgc tatacaattc acaactagtt actcgtctta
    14521 tcaaccgcgt tatgcttgat ggtaaacgtg gtactgctgc ttcaatcgtt tacggtgctt
    14581 ttgagcaaat caaagaagct actggcaacg atgcacttga agtatttgaa acagctatgg
    14641 aaaacatcat gcctgtactt gaagtacgtg cacgtcgtgt tggtggttct aactaccaag
    14701 tcccagttga agttcgtcca gaacgtcgta caacacttgg acttcgttgg ttggtaacaa
    14761 tcgctcgtct tcgtggtgaa cacacaatgc aagaccgtct tgcaaaagaa atcttggatg
    14821 ctgctaacaa cactggtgca gctgttaaga aacgtgaaga tactcaccgt atggctgaag
    14881 ctaaccgtgc attcgcacac ttccgttggt aagataggat gcgaaagcgt taagaaagtc
    14941 ccagagaaaa tagggaatcg aagcaggttg cggttgcaac caatgagatt catctttttc
    15001 tccagacttt tagcttgagc tcaactaaat catgatgcta ggcacggtaa ggatgcaagg
    15061 tgaaaatagg aaactgacgc agtattcaac gaatacaagg agttttatct ttttcacgca
    15121 gcatcccgtt ccagctcaca tcggctaact aactttagcc cgggttcaaa ttagctaaat
    15181 cgattagtat tagctataac tcagcttacc atctcgtaag ttgaaaccaa caatagcatg
    15241 aaaacattga gaacgggtag gtcctgccta tccgttttta ttaaaatcgt gttataatag
    15301 aatagaaatc aaaaataaat aggagaaaca aacctcatgg cacgcgaatt ttcacttgaa
    15361 aaaactcgta atatcggtat catggctcac gtcgatgccg gtaaaacaac aactactgag
    15421 cgtattcttt actacactgg taaaatccac aaaatcggtg aaactcacga aggtgcgtca
    15481 caaatggact ggatggagca agagcaagaa cgtggtatca cgatcacatc tgctgcgaca
    15541 acagctcaat ggaacaacca ccgcgtaaac atcatcgaca caccaggaca cgtggacttc
    15601 acaatcgaag tacaacgttc tcttcgtgta ttggatggtg cggttaccgt tcttgactca
    15661 caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aagcaactga gtacggagtt
    15721 ccacgtatcg tatttgccaa caaaatggac aaaatcggtg ctgacttcct ttactctgta
    15781 agcacacttc acgatcgtct tcaagcaaat gcacacccaa tccaattgcc aatcggttct
    15841 gaagatgact tccgtggtat cattgacttg atcaagatga aagctgaaat ctatactaac
    15901 gaccttggta cggatatcct tgaagaagac atcccagctg aataccttga ccaagctcaa
    15961 gaataccgtg aaaaattgat tgaagcagtt gctgaaactg acgaagaatt gatgatgaaa
    16021 tacctcgaag gtgaagaaat cactaacgaa gaattgaaag ctggtatccg taaagcgact
    16081 atcaacgttg aattcttccc agtattgtgt ggttcagcct tcaaaaacaa aggtgttcaa
    16141 ttgatgcttg atgcggttat cgactacctt ccaagtccac ttgacatccc agcaatcaaa
    16201 ggtattaacc cagatacaga cgctgaagaa actcgtccag catctgacga agagccattt
    16261 gcagctcttg ccttcaagat catgactgac ccattcgtag gtcgtttgac attcttccgt
    16321 gtttactcag gtgttcttca atcaggttca tacgtattga atacttctaa aggtaaacgt
    16381 gaacgtatcg gacgtatcct tcaaatgcac gctaacagcc gtcaagaaat cgacactgtt
    16441 tactcaggtg atatcgctgc tgccgttggt ttgaaagata ctacaactgg tgactcattg
    16501 acagatgaaa aatctaaaat catccttgag tcaatcaacg ttccagaacc agttatccaa
    16561 ttgatggttg agccaaaatc taaagctgac caagacaaga tgggtatcgc ccttcaaaaa
    16621 ttggctgaag aagatccaac attccgcgtt gaaacaaacg ttgaaactgg tgaaacagtt
    16681 atctcaggta tgggtgaact tcaccttgac gtccttgttg atcgtatgcg tcgtgagttc
    16741 aaagttgaag cgaacgtagg tgcgcctcaa gtatcttacc gtgaaacatt ccgcgcttct
    16801 actcaagcac gtggattctt caaacgtcag tctggtggta aaggtcaatt cggtgatgtg
    16861 tggattgaat ttactccaaa cgaagaaggt aaaggattcg aattcgaaaa cgcaatcgtc
    16921 ggtggtgtgg ttcctcgtga atttatccca gcggttgaaa aaggtttggt agaatctatg
    16981 gctaacggtg ttcttgcagg ttacccaatg gttgacgtta aagctaagct ttatgatggt
    17041 tcatatcacg atgtcgactc atctgaaact gccttcaaga ttgcggcttc actttccctt
    17101 aaagaagctg ctaaatcagc acaaccagct atccttgaac caatgatgct tgtaacaatc
    17161 actgttccag aagaaaacct tggtgatgtt atgggtcacg taactgctcg tcgtggacgt
    17221 gtagatggta tggaagcaca cggtaacagc caaatcgttc gtgcttacgt tccacttgct
    17281 gaaatgttcg gttacgcaac agttcttcgt tctgcatctc aaggacgtgg tacattcatg
    17341 atggtatttg accactacga agatgtacct aagtcagtac aagaagaaat tattaagaaa
    17401 aataaaggtg aagactaatc cgtcctcact ctagaaggaa gtcacttagt ggcttccttt
    17461 tgtctttaga aaatacctct aaatatggta aaatagtaga agaataatgt gaggaaaatg
    17521 aatgtcaaat agttttgaaa ttttgatgaa tcaattgggg atgcctgctg aaatgagaca
    17581 ggctcctgct ttagcacagg ccaatattga gcgagttgtg gttcataaaa ttagtaaggt
    17641 atgggagttt catttcgtat tttctaatat tttaccgatt gaaatctttt tagaattaaa
    17701 gaaaggtttg agcgaagaat tttctaagac aggcaataaa gctgtttttg aaattaaggc
    17761 tcggtctcaa gaattttcaa atcagctctt gcagtcctac tatagggagg ttttctctga
    17821 aggtccatgt gctagtcaag gttttaagtc cctttatcaa aatttgcaag ttcgtgctga
    17881 gggtaatcag ctatttattg aaggatctga agcgattgat aaggaacatt ttaagaagaa
    17941 tcatcttcct aatttagcca aacaacttga aaagtttggt tttccaactt ttaactgtca
    18001 agtcgagaag aatgatgtct tgacccaaga gcaggaagag gcctttcatg ctgaaaatga
    18061 gcagattgtt caagctgcca atgaggaagc gctccgtgct atggaacaac tggagcagat
    18121 ggcacctcct ccagcggaag agaaaccagt ctttgatttt caagcgaaaa aagctgcagc
    18181 taaacccaag ctggataagg cggagattac tcctatgatc gaagtgacga cagaggaaaa
    18241 tcgtctggta tttgaagggg ttgtttttga tgtggagcaa aaagtgacta gaacaggtcg
    18301 tgttttaatc aactttaaaa tgacggacta tacttcaagt ttttctatgc aaaagtgggt
    18361 taaaaacgag gaagaggccc agaagtttga cctcatcaag aagaattctt ggctccgagt
    18421 tcgagggaat gtggagatga ataatttcac acgcgatttg actatgaacg tacaggatct
    18481 gcaggaagtt gttcactatg agcggaagga tttgatgcca gaaggtgagc gtcgggttga
    18541 gtttcatgct catactaaca tgtcgactat ggatgctttg ccagaggtcg aagagattgt
    18601 tgcaacagct gctaagtggg gacacaaggc ggttgctatc acggaccatg ggaatgtcca
    18661 gtcctttcca catggctata aggcggctaa gaaagcggga atccagctga tctatggaat
    18721 ggaagccaat atcgtggagg accgtgtccc tatcgtctat aacgaagtgg agatggactt
    18781 gtcagaagca acctacgtgg tctttgacgt ggaaacgacg ggactttcag ctatctataa
    18841 tgacttgatt caggttgcgg cctctaagat gtacaagggg aatgttattg ctgaatttga
    18901 tgaatttatc aatcctgggc atcccttgtc agcttttact acagagttaa ctggaattac
    18961 agatgatcat gtcaaaaatg ccaaaccact agaacaagtt ttgcaagaat tccaagaatt
    19021 ttgcaaggat acggtcctag ttgcccacaa tgctaccttt gacgttggct ttatgaatgc
    19081 taattatgag cgtcatggtc ttccaaagat tagtcagcca gttattgata cgctggagtt
    19141 tgctagaaac ctctatcctg agtataaacg ccatggtttg gggcctttga ccaagcgttt
    19201 tggtgtggcc ttggaacatc atcacatggc caactacgat gcggaagcga ctggtcgtct
    19261 gcttttcatc tttatcaaag aggtagcaga aaaacatggt gtgaccgatt tagctagact
    19321 caacattgat ctaatcagtc cagattctta caaaaaagct cggatcaagc atgcgaccat
    19381 ctatgtcaag aatcaggtag gtctaaaaaa tatctttaag ctggtttcct tgtctaatac
    19441 caagtatttt gaaggagtgc cacggattcc gagaacggtt ctagatgccc atcgagaggg
    19501 cttgatttta ggttcagcct gttcagaggg tgaagttttt gacgtggtcg tttctcaagg
    19561 tgtggatgcg gcggttgagg tggccaagta ttatgacttt atcgaggtca tgccaccggc
    19621 tatctatgcg cccttgattg ccaaagagca ggtcaaggat atggaggaac tccagaccat
    19681 tatcaagagt ttgatagagg ttggagatcg ccttggcaag cctgttctgg ctacgggaaa
    19741 tgttcactat atcgaaccgg aagaagagat ttatcgtgaa attatcgtcc gtagtttggg
    19801 acagggtgcg atgattaacc gaactatcgg tcatggtgaa catgcccaac cagcaccact
    19861 tccaaaggct cattttcgaa cgactaatga gatgttggat gaatttgcct ttttgggaga
    19921 ggaactggct cgtaaactgg ttattgaaaa caccaatgcc ttggcagaaa tatttgaacc
    19981 cgttgaagtc gttaagggtg acttgtatac gcctttcatc gacaaggctg aagaaacagt
    20041 tgctgagttg acctataaga aagcttttga gatttatgga aatccgctgc cagatattgt
    20101 tgatttgcgg attgaaaaag aattaacatc catactgggg aatggatttg ctgtgattta
    20161 tctggcatcg cagatgctgg tgcaacgttc taatgaacgg ggttatttgg ttggttctcg
    20221 tgggtctgtc ggatctagtt tcgttgcgac catgattggg attacggaag tcaatcctct
    20281 ctctcctcac tatgtctgtg gtcagtgtca gtacagtgag tttatcacag atggttcgta
    20341 cggttcagga tttgatatgc cccataagga ctgtccaaac tgtggtcaca aactcagtaa
    20401 aaacggacag gatattccgt ttgagacctt ccttggtttt gatggggata aggttcctga
    20461 tattgacttg aacttctcgg gagaagatca gcctagcgcc cacttggatg tgcgtgatat
    20521 ctttggtgaa gaatatgcct tccgtgcggg aacggttggt acggtagctg ccaagactgc
    20581 ctatggattt gtcaagggtt acgagcgaga ttatggtaag ttttatcgtg atgcagaagt
    20641 agaacgcctc gctcaaggag cggcgggtgt caagcggaca acaggccaac acccgggggg
    20701 aatcgttgtt attccgaact acatggatgt ctacgatttt acgcctgtcc agtatccagc
    20761 agatgatgtc acggctgaat ggcagaccac tcactttaac ttccacgata tcgatgagaa
    20821 cgtcctcaaa ctcgatgtac tgggacatga tgatccgacc atgattcgaa aacttcagga
    20881 tttgtctggt attgacccta ataaaattcc tatggatgac gaaggcgtga tggcactctt
    20941 ttctgggact gatgtgctag gggtaacacc tgaacaaatt ggaacgccta cgggtatgtt
    21001 ggggattcca gagtttggaa caaatttcgt acgtggaatg gtagacgaaa cccatccgac
    21061 aacctttgcg gaattgcttc agctgtctgg tctgtcccac ggtactgacg tttggttggg
    21121 gaatgctcag gatctgatta agcaaggaat agcggaccta tcgactgtta tcggttgtcg
    21181 ggacgacatc atggtttacc tcatgcatgc gggtctggaa cctaagatgg cctttaccat
    21241 tatggaacgg gtacgtaagg gtttgtggct aaagatttca gaagaggaga gaaatggcta
    21301 tatcgaagca atgaaggcta ataaggtgcc agagtggtat atcgaatcct gtgggaaaat
    21361 taagtacatg ttccctaagg cccatgcggc agcctacgtt atgatggcct tgcgtgtagc
    21421 ttacttcaag gttcaccatc ctatttatta ctactgtgct tacttctcca ttcgtgctaa
    21481 ggcttttgat atcaagacca tgggtgcggg cttggaggtc atcaagcgca gaatggaaga
    21541 aatctctgaa aaacggaaga acaatgaagc ctctaatgtg gaaatcgatc tctatacaac
    21601 tcttgagatt gtcaatgaga tgtgggaacg aggtttcaag tttggtaaat tagatctcta
    21661 ctgtagtcag gcgacagagt tcctcatcga cggggatacc cttatcccac catttgtagc
    21721 aatggatggt ctgggagaga acgttgccaa gcaactggtg cgggcgcgtg aagagggaga
    21781 atttctctct aaaacagaac tacgcaagcg tggtggactc tcatcaacct tggttgaaaa
    21841 gatggatgag atgggtattc ttggaaatat gccagaggat aaccagttga gtttgtttga
    21901 tgagttgttt taaaaaattg cttaataatc tattaaaaga ggctaacgta tatccaatag
    21961 atttacatta gctttctttt ttgttaaaat agtctatgga aagagggtga gagtatgtca
    22021 aagatgagta taagcatccg tctggatagt gaggttaagg agcaggccca acaggtgttt
    22081 agtaatctgg gaatggatat gacaacagct attaatattt tccttcgtca ggcaattcaa
    22141 tatcagggac taccttttga tgttagacta gacgaaaatc ggaagttgct ccaagcgtta
    22201 acggatttag accaaaatcg taatatgagc cagtcttttg aatcagtctc agatttgatg
    22261 gaggacttac gtgcttaaga ttcgttatca taaacagttt aaaaaagatt ttaagttggc
    22321 tatgaagcgt ggtttgaagg cagaattatt agaagaagtt ttgaattttc tggttcaaga
    22381 aaaagaacat cctgccagat atcgtgatca ttcattgacg gcatccaagc attttcaagg
    22441 agttcgtgaa tgccataccc agccagattg gcttttggtt tataaagtag acaagtcgga
    22501 attgatttta aatttgctga ggacaggcag tcacagtgat ttattttaat ctattttaag
    22561 ggggttctca tgaaactaag aatatttgcg gaagataagc cggctaagaa ggtatttgaa
    22621 tatcaattag aacttgctga tcgtacaatt cttctatcga cagcactctt gtcaggtgct
    22681 attgctttag caggaatctt ttctgctttg agagaaaaat aaaaatagaa aagagaaaac
    22741 agaatggttt taccaaattt taaagaaaat ctagaaaaat atgcgaaatt gttggttgcg
    22801 aacggaatta acgtgcaacc tggtcacact ttggctctct ctattgatgt ggagcaacgt
    22861 gaattggcac atctaatcgt gaaagaagct tatgccttgg gtgcgcatga ggtcatcgtt
    22921 cagtggacag atgatgtgat taaccgtgag aaattcctcc atgccccgat ggagcgtttg
    22981 gacaatgtgc cagaatacaa gattgctgag atgaactatc tcttggagaa taaggctagc
    23041 cgtcttggag ttcgttcatc tgatccaggt gccttgaacg gagtggacgc tgacaagctt
    23101 tcagcttctg ctaaagctat gggacttgcc atgaagccta tgcgtatcgc aactcaatct
    23161 aacaaggtta gctggactgt agcagctgca gcaggacttg agtgggctaa gaaagtcttc
    23221 ccaaatgctg cgagcgacga agaagcagtt gatttccttt gggaccaaat tttcaaaact
    23281 tgccgtgtct acgaagcaga tcctgttaag gcctgggaag agcatgcagc tatcttgaag
    23341 agcaaggccg atatgcttaa taaggagcaa ttttcagccc ttcactacac agcgccagga
    23401 acagatttaa cacttggttt gccaaagaac catgtttggg aatcagctgg tgctgtcaat
    23461 gcacagggcg aaggattctt gccaaatatg ccgacagaag aggtcttcac agcacctgac
    23521 ttccgtcgtg cagatggtta tgtcacttct acaaaaccgc ttagctacaa cggaaatatt
    23581 attgaaggca ttaaggtgac ctttaaggat ggacaaatcg tagatatcac tgctgagaag
    23641 ggtgatcagg ttatgaaaga ccttgtcttt gaaaatgcgg gtgcgcgtgc cttgggtgaa
    23701 tgtgccttgg taccagatcc aagtccaatt tctcagtcag gcattacctt ctttaacacc
    23761 cttttcgatg aaaatgcgtc aaaccacttg gctatcggtg cagcctatgc gactagcgtt
    23821 gttgatggag cggagatgag cgaagaggag cttgaagctg cagggcttaa ccgttcagat
    23881 gttcacgtag actttatgat tggttctaac caaatggata tcgatggtat tcgtgaggat
    23941 ggaacgcggg tacctctttt ccgtaatggg aattgggcaa attaaggaga taatatgtta
    24001 ggaagtatgt tcgttggtct cctagtggga tttttagcag gtgctatgac caatcgtgga
    24061 gagcgaatgg gatgttttgg aaaaatgttt ctcggttgga tcggagcctt tctaggtcac
    24121 ttgctctttg gaacttgggg gccagtttta tcaggaacag ctattatccc agctgtttta
    24181 ggtgccatga ttgtcttagc tattttttgg agacgaggaa gttaatttcc taaatctgat
    24241 actcaatgaa aatcaaagag caaactagga aactagccgc aggttgctca aagcactgct
    24301 ttgaggttgc agatagaact gacgaagtca gtaacatata tgcggcaagg cgacgttgac
    24361 gcggtttgaa gagatttttg aagagtatga aacgaaaagg aggttggtca ttgtactagc
    24421 ctccttttga tatgatataa tagttctatg agattagata aatttttagt tgcctgtgcg
    24481 gtgggaagtc ggactgaggt caaaaacttg ctcaaggctg ggcgcgtgac tgtaaatggt
    24541 aaaaaagaaa aatcagctaa attgcagatt gatgaaaaaa tagatgagat tcgctttgat
    24601 gggcaagtgt tggagtatga agagtttgtc tactacatga tgaacaagcc caaaggagtt
    24661 atctcagcga ctgaggatcc caagcacaga accgttctgg acttgttgga tgacttggcg
    24721 cggagtaagg aagtttttcc agtaggacgc ttggatattg acacgcatgg tcttttgctc
    24781 ttgaccaata atggtcagct ggctcatgtt cttctttctc ccaagcgtca tgtggacaag
    24841 acttatcagg ctcaggtcaa gggaattatg acccaagaag atgtggagac atttatcaag
    24901 ggcattccgc tcaaagactt tacctgtcag cccgctagac tggagattct ttccacagat
    24961 gcagaaaaga atcagagcca aatccgtgtg accattgcag aagggaagtt tcatcagatc
    25021 aagcgtatgg tgggctactg tggcaaggaa gtggtggact tgcaacgatt gactatggga
    25081 actttagtat tagatgagaa cctggaacga ggggaatggc gtcgcttgac caaggaagaa
    25141 ttagaaattc tccgcgctaa tattatttaa tttagtttaa tactcaatga aaatcaaaga
    25201 gcaaactagg aaactagccg caggctgtac ttgagtacgg caaggcgacg ttgacgtggt
    25261 ttgaagagat tttcgaagag tataaattcg aaaaaggtga gggagaatgt ccttaccttt
    25321 tatgtttttt agttgcttcc tttgtgaaaa gagttataat agactgtaga ataaaaggag
    25381 gaatctatga acgcgattca agaatcattt actgataaac tatttgccaa ctatgaagca
    25441 aatgtaaaat accaagcgat tgaaaatgct gccagccaca acggaatttt tgcagctctg
    25501 gaacgtcgcc aaagccatgt agacaacaca cctgttttct cattggattt aaccaaggac
    25561 aaggtcacta accagaaagc gtctggtcgt tgctggatgt ttgcggctct caacaccttc
    25621 cgccacaaac tcatctcgca atacaaattg gagaactttg agttgtcaca agcccacact
    25681 ttcttctggg ataagtatga gaaatcaaac tggttcttgg agcaagtcat tgcgacttca
    25741 gaccaagaat tgactagccg caaggttagc ttcttactcc aaacacctca acaagatggt
    25801 ggtcaatggg atatggtcgt ttccctcttt gaaaaatacg gtgtcgtgcc taagtcagtt
    25861 tatcctgagt ctgtttcatc tagcagcagt cgtgagctaa atgcgatcct taataaattg
    25921 cttcgtcaag atgctcaaat cttgcgtgat ttgcttgttt ctggtgcaga tcaagtgact
    25981 gttcaaacta agaaagaaga cctcttgcaa gaaatcttta actttcttgc tatgtcatta
    26041 ggacttccac cacgcaagtt tgactttgct tatcgcgata aagataacaa ctacaaaagt
    26101 gaaaaaggaa tcacaccaca agagttctac aagaaatatg tcaatcttcc tttagaagac
    26161 tacgtttctg ttatcaatgc tccaactgct gataaacctt acggaaaatc ttacacagtt
    26221 gagatgttgg ggaatgtggt tggtagccgt gcagttcgct acatcaacgt tccaatggag
    26281 cgcttgaaag aattggcgat tgcccaaatg caagcaggtg agactgtttg gtttggttct
    26341 gatgtcggcc agctcagcaa ccgtaaggct ggcatccttg cgacagatgt ttatgacttt
    26401 gaatcaagca tggacattaa acttactcaa gacaaggctg gacgtttgga ctatagtgaa
    26461 agcttgatga cccacgccat ggtcttgaca ggtgttgact tggacgaaaa tggtaaatca
    26521 accaagtgga aggttgaaaa ctcatgggga gacaaggtcg gtacagatgg ttactttgtt
    26581 gcctcagacg cttggatgga cgaatacaca taccaaatcg ttgttcgtaa ggaattgctg
    26641 acagcagaag aacaagctgc ctatggagca gaaccaatcg tacttgcacc atgggatcca
    26701 atgggagcct tggctgaata aaagcataga aaaaaggaat cagattttag aacctggttc
    26761 ctttttagtt gcttgattac atgatgtgaa gaacatgtgc cacaataccc actgcgaaga
    26821 gggcaaggat aatagtgatt ggagatactt ttttcttaag taagtacatg caaagtaaag
    26881 taaggagtag tcctgataat ccaggaatca acatatccaa gttttgttgg aaagtagtaa
    26941 ctttttcagg agtttgagac aatccttgtc ctacttgtgc gaatgcttct tggatacctt
    27001 tagacccttc tggcaattta tcccaatgga tataagcctt ttcatctagt tgaactttag
    27061 aaacatcgaa agcaaattta atatttaccc agcgttgaac aaggacagca agaatgaaca
    27121 tcccaaggat agaagctcct ttagtgatat cttgaaggat accaccagac atatctttag
    27181 tgatttctga tccagccttg tatccaatct cttgaacata ccacaagaat gacatacgaa
    27241 tcaagttcca tgcaacaaag aagaggagtg gacccaagat attgccagta agggcaagtg
    27301 aagcaccgag agatccaagg attgggcgta ctgtaaacca gaatactggg tcaccgatac
    27361 cagcaagagg tcccatcata ccgattttaa ccccttggat agcagcgtca tcgatttcca
    27421 caccgttagc acgttcttct tcaagcgcaa gagtaacccc catgactgga gcagctacgt
    27481 atggatgagt gttgaagaac tcaaggtgac gctcaagagc agcgatttga tcttcttttt
    27541 tagtatagag ttttttgata gctggaatga gtgtataagc ccagcccaag ttttgcatcc
    27601 gttcaaagtt ccaagaccct tgtaagaagg ttgaacgcca ccaaactttt ttacgatctg
    27661 atttagttaa ttgaagtttt tcagtcatga tgttttcagt cctttcttat cttagtagtc
    27721 ttctaggata tcgccgattg ggtcgttaga agttgcggct cctccgccac catttccacc
    27781 agttttagaa aggtgaaggt agataagagc gatagcaaca ccgatagcac cgaatccgat
    27841 tagagtaata tctgacacag cagcgagaac gaaaccaaga gcgaagaatg gccatacttc
    27901 acgagttgcc atcatgttga taaccatggc gtaaccaacg gcaacgacca taccaccacc
    27961 gatagccata ccatctttga gccagtctgg catggcacta aggatacttt gtacagtttc
    28021 agttggtacc ataaggagaa gagctgcagg aagcgcgata cgaagtcctt ggaaaagtag
    28081 cgcaatgaaa tgcgcacgct ccacagcgcc gaagtcacct tttttagcgg cagcatctgc
    28141 agtatgaacc aaaccaactg aaattgtacg aacaatcatt gtcaagaaaa gtccagctac
    28201 ggcaagaggg atagcaaccg cttgggcaac accgatacca gtcttggtaa agtcaccacc
    28261 aagaaccatg ataatggcag cagcgacaga agcaagtgca gcatcaggag cgatagcagc
    28321 accgatattt gaccaaccaa gggcaatcat ttgaagcgat ccaccgagga taatccctgc
    28381 ttccaagtga cctgttacaa gcccaataag ggtacaggct acaagtggtt ggtgaaattg
    28441 gaactggtcg aggatgcctt caagacctgc aaagaaggct acaacgacta ctaaaaccat
    28501 agaaataata gacatgttta aaatcctttc ataaataatg gcttatttga cattggcttt
    28561 gttaatcaag tcaaacaaat cttttttaga atcatttggt actttacgga catcaaattc
    28621 aacacccaag tcacgcattt tttcaaatgt agcaacatct tctttgtcca tagacaaaac
    28681 ggtattgacc aatgttttac ctgttgagtg agccatagaa ccaacgttaa gagtcttgat
    28741 tggcacgccg ccttcgatgg cacgaagggc atcttgaggt gtttcaaaca agataagggc
    28801 atgtgtttct ccaaaacgtg ggtcttttga aatctcaatc agtttttgaa ttggaaccac
    28861 gttagccttg acattacctg gagctgcttg tttaatcaat tctttacgaa ggtcgtcttt
    28921 agccacgtta tctgaagcaa cgatgatacg atttgctttt gaatctggag tccaagcagt
    28981 tgcaacctga ccgtgaagta gacgtgtgtc aagacgggca agattgattt tcaatttacc
    29041 gtctccgata acagttcctt ctgggatagc agtttgggca actggagcag ctgcagcgct
    29101 tgcaacttct tcaactggat ttagctcttc tggaagagct ttgatgccat ctttggcttc
    29161 tttaatgata ttagcagcga ctttttctac acctgcagca gcgtccatga ggcgctctgt
    29221 gtaggcttga atcaacatcg gtaagttaag tcctgtgatg atggcaaact tacgctcagg
    29281 attttctccc atcacgcgac tagcttggtt aaatggagaa ccactccaaa ggtcagccaa
    29341 aactagaacc tcatcttctg cgtcaaatgc agcaacagcg ttattaaact tagcgtatag
    29401 atcatcagga ccttcatttg gcataaaggt cacaacttga accttttctt gttcaccaaa
    29461 gatcatagat cctgactgat gaatacccgc agcaaattcg ccgtggctcg caataatgat
    29521 tccgatactc attattgtca ttcctccttt tttgttttag ttttcattta agaaaacttt
    29581 aagactgttt aagtataaac cgttttcata aaaaaagcaa ctatttatca taaaaaatta
    29641 aaaaattttt atttttatat gttgatatgt tgagtttttg aggagtaatc ttgaattctt
    29701 atttaaaatt taatataaaa aagttggaag aagcttccaa cttttaaaat aggtttctat
    29761 tagattagtg ggtgaagtcg agtaccatac gtccttggat ttggcctttt tccatttcgt
    29821 cgaaaatggc aacagcatct tctactggac gtttttgaac aactgggact accagacctt
    29881 ctgcaccaaa ttggaaggct tcttctaagt ctttacgagt tccgacaaga gaaccgatga
    29941 cttggattcc atcgaggact gttttaacga tgctgagttc catcgtttca gaaggaagac
    30001 caacagcgac gacgcgacca ccagcacgaa tggagtcaac agcctggttg aaggcaactt
    30061 tagacacagc agttacgaca gctgaatgag ctcctccatc agttttttct ttaatgagtc
    30121 cagctacatc ttcaacttcg aggccgttaa tcacaatgtc agcgcctact tcttttgcaa
    30181 gggcaagttt gtcattgttg atatcgactg cgataacatg agcattgaat acttttttag
    30241 cgtattgaac agcgaggtta ccaagtccac cagcaccgta aagaacaacc cattggcctg
    30301 gttcaacttt tgcttctttg atagctttat aggttgttac tccagcacat gtgatagaag
    30361 aagcttgggc tggatcaagt ccgtcaggaa ctttgacagc atagtcagca gttacgatac
    30421 attgttcagc cataccaccg tctactgagt agccagcatt tttcactgta cggcaaaggg
    30481 tttcgcgacc agttgtacag tattcgcaag tgccacatcc ttcaaagaac caagcaacgc
    30541 tgacgcggtc accgacttta aggcttttca catctggagc aatctcttta acgataccga
    30601 taccttcgtg cccaagaaca cgtcctggga cttgaccaaa gtcaccatga gcaacgtgga
    30661 ggtcggtgtg gcaaacgcca cagtattcaa cttctacaag tgcttcccca gtttcaagtg
    30721 gacggagtac tttttcttca atagcaacac cagtgctttc tggatttaca acaacagctt
    30781 tcatagctat cctccttgat attgaccgag aacagggaca acgggactgg attgattttt
    30841 tgtcaacaga gtcgagtgtg acgagctctc ttgtatttat atgtgaagta tatcacaaaa
    30901 gaaagccttt tccaaggttt tggagacaaa aagtagaaca atcgattaac tggttgatga
    30961 gactgtgaaa agagcacaaa gaccagcttg caagctggtc atactcttcg aaaatctctt
    31021 caaaccacgt cagcttcatc tacaacctca aagcagtgct ttgagcaacc tacggctagc
    31081 ttcctagttt gctctttgat tctcattgag tatcagtata ggttataaga ataagtcttg
    31141 tagggttttg gcaaccccat cttggtcgtt ggtaagggaa atttgctcat ctgcataagg
    31201 aagtagctct ggattggcat ttttcatggc ataacccttc ccagcaaaag cgagcatttc
    31261 ggtatcattg tgttcatctc caaaggcaat caaatctttt ttgtcacgat tcattatctt
    31321 gagcaagtag tccaaagcaa aggccttgtt gacacctttt ggggtacatt caaggatatt
    31381 gagcggacct ccccaggtat tgatagaaag ttgatgctgg tagaaggcgt tcatgtcttt
    31441 tgccaaggca tatttgtcac tggctctggt ctgcaagagg atacagttag ggtccttggt
    31501 caccaattca ggctggaatt gatcttcagg ctggaaagct tctacaccaa atagtttggg
    31561 attggcaatc tcttcattgg gatttgtaat gtagaatttt ttacgatatt ctccagcgat
    31621 aaaatcggct tgaatgtcct ctgaacgttg aaccatatct agcagatatt ttttgtctac
    31681 agtcaaacac ttttcaaaat cccaaacttg gtctggtaaa tgagtaaggg atccgttgaa
    31741 gttaatcata ggagtgtcta agcccagttc acggtaaaaa tcttttgaca tacggtaagg
    31801 gcgacctgtc gtaataataa cctgatggcc tttttcagca acttttttga tggttttttt
    31861 ggtaaagtca gaaatttgac tgtctgagtt gagcagggtt ccatccaggt caactgcaat
    31921 aatttttttc gtcatatgga cctttctagt gtgttttcag ataagatgtc ttctattata
    31981 acagaaagca ggcagaaaag cagattccaa acaaaaaatg aaattggatt aatttcttaa
    32041 acaaatcaaa caaagacatt gaaaaagtga atgataaatg atataattct attattgttc
    32101 gtaaaaatta aaaggagatt gatgatggac aaattattta aactaaaaga gaacggtaca
    32161 gacgttcgta cagaggttct cgctggttta acaactttct ttgcaatgag ctatattctc
    32221 tttgtaaacc cacaaatact ttcacaaaca ggaatgcctg ctcagggtgt cttcctagcg
    32281 acgattattg gtgcagtagc gggtaccttg atgatggctt tttatgctaa cttaccttat
    32341 gcccaagcgc caggtatggg actcaatgcc ttctttacct ttacagttgt attcgggctt
    32401 ggttattctt ggcaagaagc cctagctatg gtcttcatct gtgggattat ttcattgatt
    32461 attaccttga caaaggttcg taaaatgatc attgaatcga ttcccaatgc tcttcgctca
    32521 gctatttcag ctggtatcgg tgtcttcctt gcctatgtag ggattaagaa tgctggactt
    32581 ttgaaattca cgattgatcc aggcaactat actgttgtag gagaaggggc tgacaaagct
    32641 caagcaacga ttgcagcaaa ctcttcagca gttccaggat tggtcagctt taataatcca
    32701 gctgttttag tggctcttgc aggacttgcc attactatct tctttgtcat caaagggatt
    32761 aaagggggaa ttattctctc tatcttgaca acaactgttc ttgctattgc agttggtttg
    32821 gttgatttgt ctagtatcga ttttgctaat aaccatgttg gtgcagcttt tgaagatttg
    32881 aagacaatct ttggtgcagc tcttggttca gaaggtttag gagctttggt ttcagataca
    32941 gctcgcttgc ctgaaactct gatggccatt cttgccttct cattgacaga tatttttgac
    33001 acaattggta ccttgatcgg tacaggtgaa aaagttggta ttgtagcgac aaatggtgaa
    33061 aatcaccaat cagccaaatt ggataaggct ctttactctg atttgattgg aacgacagtc
    33121 ggtgccattg caggtacttc aaacgtaacg acttatgttg agtctgctgc tggtatcggt
    33181 gcaggtggac gtactggttt gacagccttg gttgtagcta tctgttttgc gatttcaagc
    33241 ttctttagcc cacttctagc gatcgtacca acagcggcta cagctccaat cttgattatc
    33301 gttgggatta tgatgcttgg tagcttgaaa aatatccatt gggatgatat gtctgaagca
    33361 gttcctgcct tcttcacatc tatctttatg ggattcagct actctatcac tcaagggatt
    33421 gcagttggtt tcttgactta cactttgact aagcttgtta aaggtcaagt taaagatgtt
    33481 catgccatga tttggatttt ggatgccttg tttatcctta actacattag catggcctta
    33541 taatagaatg acccaggggg atttcccccc ttttttaata caaaggagat aggtgatgaa
    33601 agagaaaaat atgtggaaag aattgttgaa tcgtgcaggc tggattttgg tctttttact
    33661 tgccgtcctt ttatatcagg ttcccctagt ggttacctct attttgactt taaaagaagt
    33721 agccctgcta cagtcagggc tgatggttgc tggcctttca attgtggttc tggctctatt
    33781 tattatggga gctcgtaaaa ccaagttagc tagttttaat ttttcttttt ttagagctaa
    33841 agatttggca cgtttgggtt tgagttattt agttattgtc gggtcaaata tacttggttc
    33901 tatcttgttg caactgtcaa atgagacgac aacagctaac cagtctcaga ttaatgatat
    33961 ggttcaaaat agttcgttga tttccagttt cttcttgcta gccttgcttg ctccgatttg
    34021 tgaggaaatc ttgtgtcgtg ggattgttcc taaaaagatt ttccgaggca aggagaactt
    34081 gggatttgta gtcggtacga ttgtgtttgc tttattgcat caaccaagta atttaccttc
    34141 tttattgatt tatggaggta tgtcgacagt tctatcttgg acggcctaca agacccaacg
    34201 tttggaaatg tcgatcttgc ttcacatgat tgttaatggg attgctttct gtttgttggc
    34261 tcttgtggtg attatgagtc ggacattagg aatttctgtt taaaagcttt tatgtagaaa
    34321 ccgacctctt tctactaggg aaagatgaat gcaatcgtgt ccatcttttt ctttttatgg
    34381 taaaatagaa aaataataag atgaaaagcc ttgagggagt gaccgatatg tcaagtaaag
    34441 ccaatcatgc aaaaacagct atttgcggaa ttatcaatgt aaccccagac tccttttcgg
    34501 acggtggtca attttttgct cttgagcagg cactccagca ggctcgtaaa ttgatagcag
    34561 aaggagccag tatgctcgat atcggcggag aatcgactcg gccgggcagt agctatgttg
    34621 agatagaaga ggaaatccag cgtgttgttc cagtgatcaa agcgattcgc aaggaaagtg
    34681 atgtcctcat ctctattgat acttggaaga gtcaagtagc agaggctgct ttggctgctg
    34741 gtgccgatct agtcaatgat atcactggtc ttatgggtga tgagaaaatg gctcatgtgg
    34801 tagctgaagc gagagcgcaa gtggtcatca tgtttaaccc agttatggct cggcctcagc
    34861 atcctagttc gcttatcttc cctcattttg gttttggtca aacctttaca gaaaaagagt
    34921 tagcggactt tgaaacattg ccaatcgaag aattgatgga ggctttcttt gaacgagcac
    34981 tagcgagagc ggcagaagct ggtattgcac cagaaaatat cctgttggat ccaggaattg
    35041 gctttggtct gaccaagaaa gaaaatctgc ttcttttacg ggacctggat aaactacatc
    35101 agaagggcta tccaatcttt ctcggagtgt cgcgcaagcg atttgtcatc aatatcctag
    35161 aggagaatgg ttttgaagtc aatcctgaga cagagcttgg tttccgaaat cgggacacgg
    35221 cttcggctca tgtaactagt atcgctgcga gacagggtgt agaagtggtg cgcgtgcatg
    35281 acgtagctag tcacaggatg gcagttgaaa ttgcctctgc cattcgtctg gctgatgaag
    35341 cggaaaattt agatttaaaa caatataaat aagatgaaag aaattgaaaa caatcagtgg
    35401 attgctaact accggacgga tcaaccgcat tttggcttgg aacgaatggt ggaactgtta
    35461 gctttgcgtg gcaatcccca tctcaaactc aaggtcctcc atatcggagg gactaacggc
    35521 aagggctcga ctattgcttt tttgaaaaag atgctagaaa agctagggtt gagagttggc
    35581 gtgtttagct cgccctatct cattcattac acagaccaga ttagcatcaa tggggaatcg
    35641 atctcagaag cgaggctaga agctctcatg gcagactatc agtctttgct ggagggagaa
    35701 gcggtcgcca atttacaggg cacaaccgag tttgagatta tcacagccct ggcctatgac
    35761 tactttgcct cagagcaagt agatgtggcc atcatggaag ttggcatggg tggacttttg
    35821 gatagtacca atgtctgtca gcccattttg acaggaatta caactattgg cttggatcat
    35881 gtggccctac ttggtgacac cttggagtcc atagcagagc agaaggcagg tattatcaaa
    35941 caagggatgc ccttggtaac agggcgtatt gctccagaag ccttagctgt gattgaccgc
    36001 attgcggaag agaaagatgc gccgagactt gcctacggga cagattatca ggttcgtcat
    36061 caagaaagtg tggtgacagg ggaagtcttt gactatacaa gtgctgtcag acaaggttgc
    36121 ttccagacta gcctgcttgg tttgtaccaa atagagaatg ctgggatggc catagcttta
    36181 cttgatactt tttgtcaaga agatggtcga gagctagcaa gcaatgactt tcttggtcaa
    36241 gccttggaag aaacaagttg gccagggcgt ttggaaatcg tgtcaagaga tcccttgatg
    36301 attttggatg gagcccacaa tccccatgct atcaaggcct tgttggtaac cttgcaagaa
    36361 cgttttgcgg atcatcataa ggaaatcctc ttcacttgta tcaaaaccaa agccttggag
    36421 gatatgttgg acttgctggg agccatgcca gataccgagc ttactctaac acattttgcg
    36481 gatagtcggg cgacggatga aagcgtgctg aaagaggcag ctaaggctag aaatctcagc
    36541 taccaagatt ggcatgattt tctagagcag aatttgacag ataaaaaaga agagaaacaa
    36601 acagttagga ttgtcacagg ttccttgtat ttcttgagcc aagtgagggc ctatctgatg
    36661 gagaggaaga acgagaatgg atacacaaaa gattgaagca gctgtaaaaa tgattatcga
    36721 ggctgtagga gagaacgcta atcgcgaggg cttgcaggaa acacctgctc gtgtagcccg
    36781 tatgtatcaa gagatttttt caggtcttgg tcaaacagca gaggaacatt tgtcaaaatc
    36841 ctttgaaatt attgacgata atatggtggt agaaaaggat atctttttcc ataccatgtg
    36901 tgaacaccac ttcttgccat tttatggtag agcgcacatt gcctacattc cagatggtcg
    36961 tgtggcaggc ttgtctaagc tagcccgtac ggttgaagtt tattcgaaaa aaccacaaat
    37021 tcaagaacgt ttgaatatcg aagtggccga tgccttgatg gactatctag gtgctaaagg
    37081 agcctttgtt gtcattgagg cggaacatat gtgtatgagt atgcgtggtg ttagaaaacc
    37141 aggcactgca accttgacga cagtagctcg tggtctattt gaaacagata aggatctccg
    37201 tgaccaagct tatcgtttaa tggggctata aaaagaatcc gcttcaagcg gatttttcta
    37261 gaaaggaatc attatggatc aactgcagat taaggatttg gaaatgtttg cctatcatgg
    37321 tctttttcct agtgagaaag aattggggca gaaatttgtc gtttcagcca tcctatccta
    37381 tgatatgacc aaggcagcta cagacttgga tttaacagcc tctgtccatt acggagaatt
    37441 gtgtcagcag tggacgactt ggtttcagga aacgagtgaa gatttgattg aaacggtagc
    37501 ctataaactg gtggaacgta cctttgagtc ttatcctctt gtccaagaaa tgaagttgga
    37561 actgaaaaaa ccttgggcac cggtgcattt gtcactagat acttgctcgg taaccattca
    37621 tcgccgcaag caacgagcct ttatcgccct aggaagcaat atgggagata aacaagcaaa
    37681 cttgaagcaa gccattgaca aactgcgagc tcgtggcatc catattctca aagagtccag
    37741 tgtcttagcg acggagcctt ggggtggagt ggagcaggat agctttgcca atcaagtggt
    37801 tgaggtggaa acctggctac cagcacaaga cttgttagaa accttgttag ccattgagtc
    37861 agagctggga cgggtgagag aagtgcattg gggacctcgt ttgattgatt tggacttgct
    37921 ctttgtggag gaccagatcc tttatacaga cgacctcata ttgcctcatc cttacatagc
    37981 ggaacgcctt tttgtccttg agtccttaca ggaaattgcg cctcatttta tccatccgat
    38041 attaaaacaa ccgatccgca acttgtatga tgctttgaaa aaatagaaaa actctagttt
    38101 tcagtcactt gcaactgaag gctagagttt ttatactctt cgaaaatcaa attcaaacca
    38161 cgtcagcgtc gccttaccgt actcaagtac agcttgcggt tagcttccta gtttgctctt
    38221 tgattttcat tgagtattaa aataggtcat tttcttctgg gaggaggata gtttctctac
    38281 cgtccatgtc taaaaccagt actcttgggg gataacgagg gtcgaaagga tggttaaagt
    38341 caaaatcaat ggctgtaggg aggtgttgac ttgaaaagtg gaaggtaatc tttccttggt
    38401 tattaagcaa ttgaaactcg agttcttctt ccaattcaaa gacatttttt aagaaatgat
    38461 cgatgatata ccaaaaagag tcaatgatgt catcgggcaa gctggtaaca atgccaaaac
    38521 tagcagaccg catgtgggta ttggtaaaag ccatatctct gtcccctttc ttttccctta
    38581 tcatacagca aataggatta aaaatcaaga aaaggtgatt ttttgaaaag gattttaact
    38641 acagggaaaa agaaggaaaa aaattcccaa aaatctaccg aagttaatag gtaaattccc
    38701 aaattaactt gattatataa ctttcagtta ctttgagaag ttaccgaaaa atatttttca
    38761 tatctattga cttttagggg taaaatttgg tatgatagta ggcggtattg tttaccccat
    38821 ttgaaaggcc ccggaacctt ccaaatactt ttcgatggga aggaacaccc atcaccgtaa
    38881 acaaaaatcg aactatatat aggagaaatc atgaacaaaa caacatttat ggctaaacca
    38941 ggccaagttg aacgtaaatg gtacgtagtt gacgcaactg atgtaccact tggacgtctt
    39001 tctgcagtag ttgctagcgt acttcgcgga aaaaacaaac caacatttac accacacact
    39061 gatacaggtg acttcgtgat tgttatcaat gctgaaaaag ttaaattgac tggtaaaaaa
    39121 gcaactgata aaatctacta cactcactca aaccacccag gtggattgaa acaaatctct
    39181 gcaggtgaac ttcgttctaa aaatgcagta cgtttgattg agaaatcagt taaaggtatg
    39241 cttccacaca atacacttgg acgcgctcaa ggtatgaagt tgaaagtatt tgttggagct
    39301 gagcacactc acgctgcaca acaaccagaa gttcttgaca tttcagaact tatctaagga
    39361 aaggaacaat aaagtatgtc acaagcacaa tatgcaggta ctggacgtcg taaaaacgct
    39421 gttgcacgcg ttcgccttgt tccaggaact ggtaaaatca ctgttaacaa aaaagatgtt
    39481 gaagagtaca tcccacacgc tgaccttcgt cttgtcatca accaaccatt cgcagttact
    39541 tcaactgtag gttcatacga cgttttcgtt aacgttgtag gtggtggata cgctggtcaa
    39601 tcaggagcta tccgtcacgg tatcgctcgt gcccttcttc aagtagaccc agacttccgc
    39661 gattcattga aacgcgcagg acttcttaca cgtgactcac gtaaagttga acgtaagaaa
    39721 ccaggtctta agaaagctcg taaagcatca caatttagta aacgttaatt cgaaagaatt
    39781 actatactta tacagagcac ctttcggggt gttctttttt tatactttct tactaaattg
    39841 gtgcaattga cacagttgtt gcgactttag tcgcttacaa atgtggctgc aacctgacaa
    39901 ggtcagttgc ctcaaaacgt taatcaatac gattatatca atgtttcaaa gcactcaaaa
    39961 gtttacctta tttttatagt aaggtgaaat aagaatagga caaaccgagg ataatcaaat
    40021 cgaattctaa caatgtttta gaagtagagg tgtactattc tagtttcaat ctact
//
LOCUS       DAVKQB010000015        39809 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKQB010000015 DAVKQB010000000
VERSION     DAVKQB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39809
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.015"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..290
                     /locus_tag="VW528_000763"
     CDS             <1..290
                     /locus_tag="VW528_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9911612.1"
                     /translation="QYNGSWYYLNSNGAMVTGWLQNNGSWYYLNANGSMATDWVKDGD
                     TWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
     gene            complement(520..1500)
                     /gene="dusB"
                     /locus_tag="VW528_000764"
     CDS             complement(520..1500)
                     /gene="dusB"
                     /locus_tag="VW528_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9911613.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1487..2359)
                     /gene="hslO"
                     /locus_tag="VW528_000765"
     CDS             complement(1487..2359)
                     /gene="hslO"
                     /locus_tag="VW528_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9911614.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2524..3951
                     /locus_tag="VW528_000766"
     CDS             2524..3951
                     /locus_tag="VW528_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9911615.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPTFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QPPVYYLKNDLDMEDLAGIRQLLFT"
     gene            4110..5618
                     /gene="glpK"
                     /locus_tag="VW528_000767"
     CDS             4110..5618
                     /gene="glpK"
                     /locus_tag="VW528_000767"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9911616.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5660..7486
                     /gene="glpO"
                     /locus_tag="VW528_000768"
     CDS             5660..7486
                     /gene="glpO"
                     /locus_tag="VW528_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9911617.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQFGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7556..8260
                     /locus_tag="VW528_000769"
     CDS             7556..8260
                     /locus_tag="VW528_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9911618.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8557..8760
                     /locus_tag="VW528_000770"
     CDS             8557..8760
                     /locus_tag="VW528_000770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911619.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8847..9455)
                     /locus_tag="VW528_000771"
     CDS             complement(8847..9455)
                     /locus_tag="VW528_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911620.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            10547..11824
                     /gene="ltrA"
                     /locus_tag="VW528_000772"
     CDS             10547..11824
                     /gene="ltrA"
                     /locus_tag="VW528_000772"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEU9911621.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKLKLKKLTQRKWSIALTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            12282..13460
                     /locus_tag="VW528_000773"
     CDS             12282..13460
                     /locus_tag="VW528_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9911622.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANLALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            13774..13905
                     /locus_tag="VW528_000774"
     CDS             13774..13905
                     /locus_tag="VW528_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9911623.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            13926..15476
                     /gene="dltA"
                     /locus_tag="VW528_000775"
     CDS             13926..15476
                     /gene="dltA"
                     /locus_tag="VW528_000775"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9911624.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMIMDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGVTHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQSLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            15473..16717
                     /gene="dltB"
                     /locus_tag="VW528_000776"
     CDS             15473..16717
                     /gene="dltB"
                     /locus_tag="VW528_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9911625.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIATTLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGTIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALGISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            16731..16970
                     /gene="dltC"
                     /locus_tag="VW528_000777"
     CDS             16731..16970
                     /gene="dltC"
                     /locus_tag="VW528_000777"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9911626.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            16963..18231
                     /gene="dltD"
                     /locus_tag="VW528_000778"
     CDS             16963..18231
                     /gene="dltD"
                     /locus_tag="VW528_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9911627.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            18386..18826
                     /gene="adcR"
                     /locus_tag="VW528_000779"
     CDS             18386..18826
                     /gene="adcR"
                     /locus_tag="VW528_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9911628.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            18826..19530
                     /locus_tag="VW528_000780"
     CDS             18826..19530
                     /locus_tag="VW528_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9911629.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            19523..20329
                     /locus_tag="VW528_000781"
     CDS             19523..20329
                     /locus_tag="VW528_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9911630.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            20339..21844
                     /gene="adcA"
                     /locus_tag="VW528_000782"
     CDS             20339..21844
                     /gene="adcA"
                     /locus_tag="VW528_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9911631.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(23015..23788)
                     /locus_tag="VW528_000783"
     CDS             complement(23015..23788)
                     /locus_tag="VW528_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9911632.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            23964..25367
                     /locus_tag="VW528_000784"
     CDS             23964..25367
                     /locus_tag="VW528_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9911633.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDVDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELNSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            25482..26120
                     /locus_tag="VW528_000785"
     CDS             25482..26120
                     /locus_tag="VW528_000785"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU9911634.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            26132..26572
                     /locus_tag="VW528_000786"
     CDS             26132..26572
                     /locus_tag="VW528_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU9911635.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            26565..26996
                     /locus_tag="VW528_000787"
     CDS             26565..26996
                     /locus_tag="VW528_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9911636.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            26987..27457
                     /locus_tag="VW528_000788"
     CDS             26987..27457
                     /locus_tag="VW528_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9911637.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            27482..28255
                     /locus_tag="VW528_000789"
     CDS             27482..28255
                     /locus_tag="VW528_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9911638.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            28252..29052
                     /locus_tag="VW528_000790"
     CDS             28252..29052
                     /locus_tag="VW528_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9911639.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            29111..31405
                     /locus_tag="VW528_000791"
     CDS             29111..31405
                     /locus_tag="VW528_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9911640.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRV
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            31415..34531
                     /locus_tag="VW528_000792"
     CDS             31415..34531
                     /locus_tag="VW528_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9911641.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            34588..36354
                     /locus_tag="VW528_000793"
     CDS             34588..36354
                     /locus_tag="VW528_000793"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9911642.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            36500..37651
                     /locus_tag="VW528_000794"
     CDS             36500..37651
                     /locus_tag="VW528_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9911643.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            37796..38680
                     /locus_tag="VW528_000795"
     CDS             37796..38680
                     /locus_tag="VW528_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9911644.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(38798..38875)
                     /locus_tag="VW528_000796"
                     /pseudo
     CDS             complement(38798..38875)
                     /locus_tag="VW528_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            39079..39330
                     /locus_tag="VW528_000797"
     CDS             39079..39330
                     /locus_tag="VW528_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9911645.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFDGMGDQPVQDTE
                     TSSALILSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            39318..39608
                     /locus_tag="VW528_000798"
     CDS             39318..39608
                     /locus_tag="VW528_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911646.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
BASE COUNT        12313 a         6883 c         8638 g        11975 t
ORIGIN      
        1 tccaatacaa tggttcatgg tactacctca acagcaatgg tgctatggta acaggatggc
       61 tccaaaacaa tggctcatgg tactacctaa acgctaacgg ttcaatggca acagattggg
      121 tgaaagatgg agatacctgg tactatcttg aagcatcagg tgctatgaaa gcaagccaat
      181 ggttcaaagt atcagataaa tggtactatg tcaatggctc aggtgccctt gcagtcaaca
      241 caactgtaga tagctataga gtcaatgcca atggtgaatg ggtaaactaa acttaatata
      301 actagttaat actgacttcc tgtaagaact ctttaaagta ttccctacaa ataccatatc
      361 ctttcagtag ataatatacc cttgtaggaa gtttagatta aaaaataact ctgtaatctc
      421 tagccggatt tatagcgcta gagactacgg agtttttttg atgaggaaag aatggcggca
      481 ttcaagagac tctttaagag agttacgggt tttaaactat taagccttct ccaattgcaa
      541 gagggcttca atctctgcta gggtgctagc ttgcgaaatg gctccacgga gtttggcagc
      601 gccagatgtt ccacggaggt agtgaggagc gaggccgcgg aattcacgaa ctgcgacgtt
      661 ttctcctttg aggttaatca atcgtttcaa gtgttcgtag gcgatcttca tcttgtcttc
      721 aaaggtcaaa tcaggtagga tttctcctgt ttcaaagtaa tggttgattt ggttgaagag
      781 gtaaggattt cccatggcag ctcggccaat catgactgcg tcagcaccaa cttcttcgat
      841 gcgttgcttg gcttcttgga cagtacggat atcaccgttg gcgatgaatg gaatcttggt
      901 tagagcttgg gcaaccttgt aaagggtctc aaggtctgcg tggccagtat acatttgttc
      961 acgggtacgg ccatgcatgg cgagggcaga aacacctgca gcttcagcag cgagggcatt
     1021 ttctactgcc agagatgggt ccgcccagcc ggtacgcatt ttgacagtaa gtgggatatc
     1081 aaggacagac tggaccttgt tgatgataga gtagatcttg tcaggatcct tgagccacat
     1141 agcaccagct tcgttcttca cgattttgtt gacagggcag cccatgttga tatcgacgat
     1201 atcggtcttg gtgttttctt ggatgaattc tgctgcgcgt gctaggctgt cttcatcgct
     1261 accaaaaagt tggatagaga cagggttttc gccctcatcg atatgaagca tgtgcagggt
     1321 tttttcgttg ttgtattgga ttcccttgtc agagaccatt tccattacaa cgagtccagc
     1381 tccgagctct tttgcgatgg tacgaaaggc tgagttggtc acgccagcca taggcgctaa
     1441 aacggtacga ttgggaatct caacattgcc aatcataaaa ggtgtattaa gatttgtcac
     1501 gaatgagttc ctccaggtcc ttttcatcaa agttgtaagt agtttggcag aattgacaag
     1561 tgatttctgc cccgtggtct tcctctttca tttcctgtaa gtctgagctt ggaaggctgg
     1621 caagagcgtt cataaagcgt tcatggctac agtcacattg gaaacggatt tcttcttcag
     1681 aaagacgctt gtaggcttcg tccccgtaga tagccttgag gagggcttcg atatggtcgt
     1741 cgctttcgag aagagtagag atagctggca tttcttggat gcgtttttca aagcgagcaa
     1801 tctcttcttt cttggctcct ggcaagactt gaactaggaa accacctgca accttgacct
     1861 tgtcttcctc gtccaaaagg acattgaggc cgaccgctga aggcgtttgt tggctttcag
     1921 taaggtaaaa ggcaaggtct tcaccgattt ctccagagat gaggggagtt atagagttgt
     1981 aaggatttcc agcaccgtag tctgtgataa cgaggaattg accatttcca acaaaaggtc
     2041 cgactaggac ttcaccagtc gcagtctttt tgatgtcaac accaggattt tgaacatagc
     2101 ctttgacgtt ccccttggta tcagcgacgg tgataatagc acctagagag ctagagccca
     2161 acaccttaac tgtaagtttg gtatttcctt tttcattggc tgcgagaatc tggctggcga
     2221 taagagttcg accaagcgct acagttgagc tagcttgggt ttgatgtttt tcttgagcag
     2281 tgcggacggt ttccgtgcta tcaaggacaa aagcacgaaa ggctccgctt tctgatatag
     2341 ttttaataat tttatccata gctactattt tagcataaaa atgcccaaag ggggagccgt
     2401 gtgtttactg attttcagga taatggacca ggaaatcagc atgaaaataa aaagagaaac
     2461 agattatttt agcatttgta agatttatgc tatgcttaag gtagaaaatg aaaggggtaa
     2521 caaatgtatt taggagattt gatggagaaa gccgagtgtg gtcaattttc aatcctttcc
     2581 tttctattac aagagtctca gacgaccgtc aaggctgtaa tggaagaaac aggattttca
     2641 aaagcaaccc taaccaaata tgtcaccctg ctcaatgaca aggctttgga tagtggctta
     2701 gagctggcta ttcactcaga agatgaaaat ctgcgtctgt ctatcggtgc agctaccaag
     2761 gggagagata ttcggagctt gtttttggag agtgctgtta aataccagat tttggtttat
     2821 cttctctacc accaacagtt tttagcccat cagctggctc aagaattggt gattagcgag
     2881 gctacgcttg gtcgtcactt ggctggttta aatcagattt tgtcagaatt tgatttatcc
     2941 atccaaaatg gacgttggcg aggtccagag catcagattc gctatttcta tttctgtctt
     3001 ttccgaaagg tctggtcgag tcaggaatgg gaaggtcaca tgcagaaacc agagagaaaa
     3061 caggagattg ccaatttaga ggaaatctgc ggtgcaagtt tgtctgtggg gcagaaattg
     3121 gacttggttc tctgggctca catcagtcaa caacgtcttc gggtcaatgc ttgtcagttt
     3181 caagtcatag aagagaaaat gcgagggtat tttgacaata ttttctacct tcgtttgctg
     3241 agaaaggttc cgtccttttt tgctgggcaa catattccac taggagttga ggatggtgag
     3301 atgatgatat tcttctcttt tctcctatct catcgcattc ttcctcttca tactatggag
     3361 tatattcttg gttttggagg gcagttggca gatttactga cgcaattgat tcaagaaatg
     3421 aagaaggagg aactattggg ggattataca gaggaccatg tcacctatga actcagtcag
     3481 ctttgtgctc aagtctatct ctataagggc tatattttac aggatcgcta caagtaccag
     3541 ttagagaatc gtcatccata tttactgatg gaacatgatt ttaaagagac agcagaggag
     3601 atttttcatg ctctacctac ttttcaacag gggacagatt tagataagaa gattctctgg
     3661 gaatggctcc agttaatcga atatatggct gaaaacggtg gccagcatat gcggattggt
     3721 ctggatttga catctggttt tcttgtcttt tcaaggatgg cagccatttt gaaacggtat
     3781 ttggaataca atcgttttat taccattgaa gcttatgacc ctagtcggca ttatgatttg
     3841 ctggttacca ataacccgat tcataagaag gaacagcccc cagtttatta tttaaaaaat
     3901 gacttggata tggaagattt ggcagggatt cgtcagttat tattcactta aaaggcttgg
     3961 ttaatccagg tcttttttgt gaaattcaca caatctcctc acattttttt aaaaattaaa
     4021 aaaagttgat aaacaagaaa gcgctttatt ttgtatacta gtaagtgtaa agaggaaaca
     4081 cctcaagatc tttatcagga ggacagtata tgtcacaaga aaaatacatc atggccattg
     4141 accagggaac tacaagttct cgtgctatca ttttcaacaa aaaaggggaa aaggttagct
     4201 cgagtcaaaa agagtttacc cagattttcc ctcaggcagg ttgggttgag cacaatgcca
     4261 atgaaatttg gaactctgtt cagtcagtta ttgcgggtgc tttcatcgaa agtggtgtca
     4321 agccaaatca aatcgaggca atcgggatta ccaaccaacg tgaaacaacg gttgtctggg
     4381 ataagaaaac aggacttcct atctacaatg ctatcgtttg gcagtcacgc cagacagcac
     4441 ctttggctga gcaactaaaa agccaaggtt atgtggaaaa attccatgaa aagactggtt
     4501 tgattattga tgcttacttc tctgctacca aggttcgttg gattttggat catgtagaag
     4561 gcgctcaaga gcgagcagaa aaaggggaat tgctctttgg tactatcgat acttggttgg
     4621 tttggaaatt gactgacggt gcggctcacg tgactgacta ctcaaatgca gctcgtacca
     4681 tgctttataa cattaaagaa ctcaaatggg atgatgaaat tttggaaatc cttaacattc
     4741 cgaaggctat acttccagaa gttcgttcta actccgaaat ctacggcaag acagctccat
     4801 tccatttcta cggtggagag gtgccaatct caggtatggc tggggaccaa caagcagccc
     4861 tctttggaca gttggctttt gagccaggta tggttaagaa tacttatgga acaggctctt
     4921 tcatcatcat gaatactggg gaagagatgc agttgtctga aaacaacctc ttgacaacca
     4981 ttggttacgg aatcaacggt aaggtttatt atgccttgga aggttctatc ttcatcgcag
     5041 gaagtgctat tcagtggctt cgtgacggtc ttcgcatggt tgaaaattca ccagaatctg
     5101 aaaaatacgc tcgtgattct cacaacaacg atgaagttta tgtcgttcca gcctttacag
     5161 gtctaggcgc tccatactgg aaccaaaatg ctcgtggttc cgtctttggt ttgactcgtg
     5221 gaacaagcaa agaagacttt atcaaggcga ctttgcaatc tattgcttat caagtgcgtg
     5281 acatcatcga caccatgcaa gtggatactc agaccgccat tcaagtactg aaggtggatg
     5341 gtggtgcagc catgaacaac ttcctcatgc aattccaggc ggatatttta ggcattgaca
     5401 ttgcacgtgc taaaaacttg gaaacaacag ctctaggagc ggccttccta gcaggtttgt
     5461 cagtagggta ctggaaagac ttggacgagt tgaaactctt gaacgagaca ggagaactct
     5521 ttgagccatc tatgaatgaa tctcgcaagg aacaactcta caagggctgg aagaaggctg
     5581 tgaaagcaac tcaagtcttt gcggaagtag acgactaata ctggcagaat aaagcgattt
     5641 atttagaaag tgtgtaaata tggaattttc aaagaaaaca cgtgaattgt caattaaaaa
     5701 aatgcaggaa cgcaccctgg acctcttgat tatcggtgga ggaatcacag gagctggtgt
     5761 agccttgcag gcggcagcta gcggtcttga gactggtttg attgaaatgc aagactttgc
     5821 agaaggaaca tctagtcgtt caacaaaatt ggttcacgga ggacttcgtt acctcaaaca
     5881 atttgacgta gaggtggtct cagatacggt ttctgaacgt gcagtggttc aacaaatcgc
     5941 tccacacatt ccaaaaccag atccaatgct cttaccagtt tacgatgaag atggagcaac
     6001 ctttagcctc ttccgtctta aagtagccat ggacttgtac gaccttttag caggtgtgag
     6061 caacacacca actgcgaaca aggttttgag caaggatcaa gtcttggaac gccagccaaa
     6121 cttgaagaag gaaggcttgg taggaggtgg agtgtatctt gacttccgta acaacgatgc
     6181 gcgtctcgtg attgaaaaca tcaaacgtgc caaccaagac ggtgccctca ttgccaacca
     6241 cgtgaaggca gaaggcttcc tctttgacga aagtggcaag attacaggtg ttgtagctcg
     6301 tgatctcttg acagaccaag tatttgaaat caaggcccgt ctggttatta atacaacagg
     6361 tccttggagt gataaagtac gtaatttgtc taataaggga acgcaattct cacaaatgcg
     6421 cccaactaag ggagttcact tggtagtgga ttcaagcaaa atcaaggttt cacagccagt
     6481 ttactttgac acaggtttgg gtgacggtcg tatggtcttt gttctcccac gtgaaaacaa
     6541 gacttacttt ggtacaactg atacagacta cacaggtgat ttggagcatc caaaagtaac
     6601 tcaagaagat gtagattatc tacttggcat tgtcaacaac cgctttccag aatccaacat
     6661 caccattgat gatatcgaaa gcagctgggc aggtcttcgt ccattgattg cagggaacag
     6721 tgcctctgac tataatggtg gaaataacgg taccatcagt gatgaaagct ttgacaactt
     6781 gattgcgact gttgaatctt atctctccaa agaaaaaaca cgtgaagatg ttgagtctgc
     6841 tgtcagcaag cttgaaagta gcacatctga gaaatatttg gatccatctg cagtttctcg
     6901 tgggtctagc ttggaccgtg atgacaatgg tcttttgact cttgctggcg gtaaaatcac
     6961 agactaccgt aagatggctg aaggagctat ggagcgcgtg gttgacatcc tcaaagcaga
     7021 atttgaccgt agctttaaat tgatcaattc taaaacttac cctgtttcag gtggagaatt
     7081 gaacccagca aatgtggatt cagaaatcga agcctttgcg caatttggag tatcacgtgg
     7141 tttggatagc aaggaagctc attacctagc aaatctttac ggttcaaatg caccgaaggt
     7201 ctttgcactt gctcacagct tggaacaagc gccaggtctt agcttggcag acaccttgtc
     7261 ccttcactat gcaatgcgca atgagttgac tcttagccca gttgacttcc ttcttcgtcg
     7321 taccaatcac atgctcttta tgcgtgatag cttggatagt atcgttgagc caattttgga
     7381 tgaaatggga cgattctatg actggacaga agaagaaaaa gcaacttacc gtgctgatgt
     7441 cgaagcagct ctcgctaaca acgatttagc agaattaaaa aattaagaaa aaataaaaga
     7501 ggtggagggc agcattcctt gtcgcccgtc ccttcttttt aatggagaca gaaagatgat
     7561 gaatgaatta tttggagaat ttctagggac tttaatcctg attcttctag gaaatggtgt
     7621 tgttgcaggt gtggttcttc ctaaaaccaa gagcaatagc tcaggttgga ttgtgattac
     7681 tatgggttgg gggattgcag ttgcggttgc agtctttgta tctggcaagc tcagtccagc
     7741 tcatttaaac ccagctgtga ccatcggtgt ggccttaaaa ggtggtttgc cttgggcttc
     7801 cgttttgcct tatatcttag cccagttcgc aggggccatg ctgggtcaga ttttggtttg
     7861 gttgcaattc aaacctcact atgaggcaga agaaaatgca ggcaatatcc tggcaacctt
     7921 cagtactgga ccagccatca aggatactgt atcaaacttg attagcgaaa tccttggaac
     7981 ctttgttttg gtgttgacaa tctttgcttt gggtctttac gattttcagg caggtatcgg
     8041 aacctttgca gtgggaactt tgattgtcgg tatcggtcta tcactaggtg ggacaacagg
     8101 ttatgccttg aacccagctc gtgaccttgg acctcgtatc atgcacagca tcttgccaat
     8161 tccaaacaag ggagacggag actggtctta cgcttggatt cctgttgtag gccctgttat
     8221 cggagcagcc ttggcagtgc ttgtattctc acttttctag tttatactct tcgaaaatca
     8281 aattcaaacc acgtcagcgt cgccttaccg tactcaagta cagcttgcgg ctagcttcct
     8341 agtttgctct ttgattttca ttgagtatta gaaaacaatt atgttgatag agcttgggca
     8401 agagcccaat ttcagcaaaa aatgaagtaa atcttctcat aataaaacgc atcatatcaa
     8461 gcacgaaaat tccacgaggt caactacagt cagaaagctg aacaacaagc caaaacgccc
     8521 aaaaaaggcg gcaaaaagca agcacctgca agcaacgtgc cgaaatggtc aaatcctgat
     8581 tatgtcaacg aattagaccc aaaaatcgtt gatatgctag tagaatttca caagtcacaa
     8641 ggcactttgg aaactcccga ggcgcaagca gaaatcgccc aaaaacgtga agaaatcgag
     8701 caaaggagag ctgagcttga ggataaaaaa caagagcttt tgaaccgctt gaacaaatag
     8761 agtttcgcaa gtattatgct tacaaattac ttgagcaatt aactaaaata taaaccctgc
     8821 ctttatatct aggcagggtt tatattttag aaattcacgt aggttgttac ggtttttaca
     8881 tacccagtat agtttgagtt tctatagtat tcagtgataa acttccattt tctttgagca
     8941 acatggatat aagtacttgt tatgtagtat ggatatgggc tttgtgaatc caagtaagac
     9001 tgataagctt gtataccaaa atatgctcca ccaattattg caccccatgg accccccaat
     9061 aaagcaccta tcctaccaat catataactg attccagcac cagtcatgaa gttagcgaat
     9121 gtgttagctt gtttattccc atgtattgtg ttgacgtaat tccaaacatt aggatcgtat
     9181 gatctaaaag atatatttag gtcgatttca ttcttttgat aagccatata aaatgcccca
     9241 ttgatataga cgccgtcagc acgtcgttca atagtgtcta cacttccatc tggattgaca
     9301 acctcaagaa cttcatcgct taaaatattt acttgcgtat ctccgaaccg cactgatgag
     9361 ccattctcaa actgagcctc accagataca actttagagt ttgccgataa gctatcatca
     9421 gcaaaaacaa caagcgacgg ggaaatgcta gacatacaga aaacagacat aactagcaaa
     9481 cacatgcatt taaacatctt agacataacg gaaactcctt tgtatttttg atttttttta
     9541 acttttatta tacaataaaa ccaaataaaa agaaagcggt aacaatatgc ttaatgcgaa
     9601 aattttttat atatttttat gtttgatcgt tatcgaaact acaggcttgt tgttgttgaa
     9661 aagaggtctc gaaatgggtt atttagacac agaagctatt atcctcgcag ttttttcatt
     9721 tgctttttac aacctatgtt cattcgcttg ggtctgctct acaataaaaa acaataaaaa
     9781 ataaatagac gtattttcaa aaaaaaaaaa aaaatgcata tttatcttag caaaacgacg
     9841 atttaaatcg tcgttttttt gtagtacgac gggcatgtcg tatatctgag gtgtaagtcc
     9901 tcggtgggca cctgctaccg gtgaacccaa tagcgattcc caagcctgac tatcgtgagg
     9961 tagcagggag aggaagggat agcgaaatcg tggctctacg aacaggaacg tgatagtaag
    10021 gcgtatatag cggataagga ggcttcaaac tctaaagtcc aaaaaggtag tcgtaaccta
    10081 tatgtgtaaa tcacgagagt aattgaattc ggactaaggt ttgtgtgaaa aagataaatc
    10141 tttctagagt ctaaagactc tgcgtcagat ttcctatttt cactgtaacc ttttaacgtc
    10201 ctcatatctt gtataaacga ggaaagatgt acgacttatc ccgtgaggtt tcatgagcgc
    10261 tgaaagcgta gtaacaacga atcatgagaa gtcagccgag cccatagtag tgaggaaatt
    10321 tccgtaatgg aagtggagcg aaggggtgaa tactcaaaca gtctggggag agactgtttg
    10381 aggtctgtcg ctagaaagag aaaacgacag atcgaagtaa tcctacttca cttgtgtctg
    10441 taaaatgagt ggtctgatag aactggactt tgacacgtaa tgactagatg aaggttcacc
    10501 aatataagat gtccctcagg caccaaaaca agaaaggaat aggcacatgt caaaactgct
    10561 agataagata ttatcacgcg aaaatatgct ggaagcctac aatcaagtaa aatccaataa
    10621 aggctcagct gggattgatg gaatgactat cgaagagatg gataattatc tcagacaaaa
    10681 ctggcgcttg actaaggaac tgataaaaca gagaaaatat aagcctcaac cagttcttaa
    10741 agttgagata cctaaaccag acggaggcat ccgtcaacta ggaattccaa cagttatgga
    10801 tagaatgatt caacaggcca ttgtccaagt tattagcccc atttgtgaac cccatttctc
    10861 agatacgagt tatggcttca gaccaaatag gtcatgtgaa aaagccatca tgaagctctt
    10921 agaatactta aatgacggct atgagtggat agtggatata gacctagaga aatttttcga
    10981 tacagttcct caagatagat tgatgtcctt agtacataac attatcgaag acggagatac
    11041 ggaatccttg attcgtaagt atcttcattc aggtgttatc attaatggtc aacgttataa
    11101 aacactagtt ggtacaccac agggaggaaa tttatctcct ctcttatcca atatcatgct
    11161 taatgaattg gacaaggaat tagaaaagag gggacttcga tttgtgcgct acgcagatga
    11221 ttgtgtgatt acggtcggaa gcgaggcagc cgctaagcgt gtgatgtatt cagtcagtcg
    11281 ttttattgag aaacggctag gtttgaaagt aaacatgacc aagactaaga ttaccagacc
    11341 aagagagttg aaatatctag gttttgggtt ctggaaatca tcagatggtt ggaaaagccg
    11401 tccacatcaa gatagtgttc ggagatttaa gcttaaattg aagaaactaa cacagaggaa
    11461 atggagtata gctctaacaa gacgtattga gcaactgaat ttgtctattc gaggatggat
    11521 aaactatttc tcattgggaa atatgaaaag tatagtcgcc agcatagatg agcgcttgcg
    11581 tactcgccta cgagtgatta tctggaagca atggaagaag aaatcgagac gattatgggg
    11641 attgcttaag ttaggagttc ctaaatggat agcagataag gtatctggct ggggcgacca
    11701 ttatcaatta gtagctcaga agtcggtact taaacgtgct atatcaaaac cagtccttga
    11761 aaaacgtgga ctggtttcgt gtttggatta ttaccttgaa cgacatgcgt taaaagttag
    11821 ttgaaccgcc gtatgccgaa cggcacgtac ggtggtgtga gaggggctag agattatccc
    11881 ctactcgatt aactcccctg aaatttattt taattatgca aatttcacgt atttttgatg
    11941 ctgagacgac gatcctggga acttttcaga tatttttttg actatctaaa atctatcatt
    12001 agaaaagctt agagcgccaa aggatttgag cgtttttctg atttttaaga ctttttccca
    12061 gtctcttttt cgattgaaga tgtaattatt ctactaacta actaacttct tagtactagc
    12121 caacaacgat aatcataatt cctcctaaaa ttaggaataa taaaggcaat agtttttgtt
    12181 ttttcatgta aaaaacctca cttttgtttt ctgctatttt atgctaaaat attaaaaatc
    12241 aaatttaatt ccaaagtttg taactaaagg gggagcgcta catgtctaat tcatttgtca
    12301 agttgttagt ctctcaatta tttgcaaatt tagcagatat tttctttaga gtaacaatca
    12361 ttgctaacat atacattatt tcaaaatcag taattgccac atcactagtt cctatcttaa
    12421 taggaatatc ctcttttgtt gcgagtcttt tagttccgtt ggttactaaa aggttagcgc
    12481 taaatagggt tttatcttta tctcaatttg gaaagactat attattggcg atactggtag
    12541 gaatgtttac cgtaatgcaa tccgtagcgc ctttggtgac ctatctattt gttgttgcaa
    12601 tttccatact agatggtttt gcagcacccg tttcctatgc tattgtgcca cgctatgcga
    12661 ccgatttggg taaggctaat ttagccttat caatgactgg tgaagctgtt caattgatag
    12721 gttggggatt aggtggactc ttgtttgcaa caattggtct gttacctacc acgtttatca
    12781 ttttagtctt gtatatcatt tctagctttc tgatgttatt tcttcctaac gctgaagtgg
    12841 aggtgttaga gtcagaaact aatcttgaaa ttttgctcaa aggttggaag ttagttgcta
    12901 gaaatcctag attaagactt tttgtatcag caaatttatt ggaaattttt tcaaatacga
    12961 tttgggtttc ttccattata cttgtttttg taacggagtt attaaataaa acggaaagtt
    13021 actggggata ttctaataca gcatactcta ttggtattat aattagtggc ttaattgctt
    13081 ttaggctatc tgaaaagttc cttgctgcta aatgggaaag tattcttttt cctcttgtag
    13141 ctatggcaat agtgacactg actattctat attttccaaa cgcacagatg tttttattat
    13201 tttcagcttt agttggtatg ttatcgcaac taaaagaagt tcctgaaagt gtatttcttc
    13261 aggaaacagt agaggaaaat catttagtta atgtctattc cgttcttgaa gtgatttcta
    13321 cattagcatt ttcagtattt gttttgctaa tgagctatat tactgagagt tttggtatta
    13381 gcatcagttt ttggctatca gccatttgtc tgatgataga agctatttta atttatatca
    13441 gacgagatta ttttaaatga gagagtggtt aaaaagtctt taaaaatcag aaaagcgcat
    13501 agtatcaggt gttgaaaaaa cttgatattg tgtgttttat tatgggaaga tttacttcat
    13561 tgtctcctga aatggagttt tgaagcagga tcactctatt tgaaaatata catagaaaaa
    13621 ccgcataatc cttaaaataa gctgaaatct attggaaaca tacggtaaac aattgaaaag
    13681 atactatata aagtggtaca atggtagaaa aataagctag taagaatcac tcttatttta
    13741 gcgaaaaatt acagaaatga atggtttttc ttgatgaaac agagaaaaga attgtacctc
    13801 tttcttggtc ggacagcctt gtattttctt atctttctag ggctgcttta cttctttagc
    13861 tatcttggtc agggtcaagg aagctttatc tataatgaat tttaacttga aggagaatcc
    13921 ctattgtgtc aaataaacca atagcagata tgattgaaac cattgagcat tttgctcaga
    13981 cacagcctag ctatcctgtc tataatgttt tggggcagga acacacttat ggcgatttaa
    14041 aggctgattc ggatagtttg gctgcagtca ttgaccaact aggcttgcct gagaagtctc
    14101 ctgtggttgt ttttggtggc caagaatatg aaatgttggc aacctttgta gcgctgacta
    14161 agtcaggtca tgcctacatt ccaattgata gccattcggc cttggagcga gtttcagcta
    14221 ttttagaagt agcagagcca agcttgatta ttgccatttc agcctttccc ttggagcagg
    14281 tttctacacc aatgataaat ctagctcagg ttcaagaagc ctttgcccaa gggaacaact
    14341 atgaaatcac gcatccagtc aagggagatg ataattacta cattatcttt acttctggta
    14401 cgactggtaa gcctaaggga gtgcagattt cacatgataa tctcctcagc tttacaaact
    14461 ggatgattat ggataaggaa tttgcgacac cgagtcgtcc gcaaatgctg gcacagccac
    14521 cttattcttt tgacttgtct gtcatgtatt gggcaccgac cttggcactt ggtggtacgc
    14581 ttttcactct tccttcagtc atcactcagg actttaagca actctttgcg gctatctttt
    14641 cattgccaat cgctatctgg acatcaacac catcctttgc agatatggcc atgttgtctg
    14701 aatacttcaa cagtgagaaa atgcctggag tcacgcattt ctactttgat ggtgaagaat
    14761 tgacggtcaa aacagctcaa aaactgcgcg agcgtttccc aaatgcccgt atcatcaatg
    14821 cttacggccc aacagaagcg acagtagctc tgtcagcagt tgccgtgaca gacgagatgt
    14881 tagcgactct caaacgccta ccaatcggct ataccaaggc tgattctcca acctttatca
    14941 ttgacgagga aggaaataaa ctgccaaatg gcgaacaggg agaaatcatt gtttctgggc
    15001 cagctgtttc aaaaggttat atgaacaatc ctgaaaaaac agcagaagcc ttctttgagt
    15061 ttgaaggtct gccagcctat cacacaggcg atgtgggaac catgacagat gagggcttgc
    15121 ttctctacgg cggacgcatg gacttccaga ttaagtttaa cggttaccgc attgagttag
    15181 aagatgtctc tcaaagcctc aacaagtctc gctttatcga atctgctgtc gcagtaccgc
    15241 gctataacaa ggaccacaag gtacaaaatc tattggctta tgtcatctta aaagacggtg
    15301 ttcgtgagca gtttgagcga gatatcgata ttaccaaggc catcaaggaa gacctgacag
    15361 acatcatgat gtcctacatg atgccatcta aattccttta ccgagacagt ttgccactaa
    15421 ctccaaatgg aaagattgac atcaaaggat tgattaacga ggtgaataag agatgatgga
    15481 gttttttcaa cagcttcctc atttagagcc atatggcaat cctcagtatt ttgtttatgt
    15541 gattgctaca accttgccca tctttatagg cctctttttc aagaaacgct ttgcctggta
    15601 tgaagtgttg gtaagtctct tctttattgt caccatgttg gtgggtggaa agaccaatca
    15661 actagctgcc ttgggtattt acctttgctg ggaaatattg ctcctgcttt tctacaagca
    15721 ttatcgaaaa agcaaggatg gcaagtgggt cttctactta gttagttttc tgtccctact
    15781 tccgattatc tttgtcaagg tgcaaccagc tatcaatgga acgcagtctt tgcttgggtt
    15841 cttgggaatt tcttacctga cctttcgttc ggttgggact atcatcgagc tgagagatgg
    15901 agtgattaag gattttaccc tctgggaatt cctccgtttc cttctcttca tgccaacttt
    15961 ctcgagtggt ccaatcgatc gctttaagcg atttaatgaa aattatcagg ccattcctga
    16021 gcgagatgag ttgatggata tgctggatga atctgtccgc tatatcatgt ggggcttttt
    16081 gtataagttt atcctagctc atgttttagg agagacctta ctacctcctc tgaagaattt
    16141 agccttgcag tcaggtggct tctttaatct ctatgccttg gcagttatgt atacttttgg
    16201 tctggagctc ttctttgatt ttgcaggtta ttctatgttt gctttgggca tctcaaactt
    16261 gatgggaatc cgtagcccta tcaactttaa caagcccttt ttatcaaggg atttaaagga
    16321 gttttggaat cgctggcata tgagtctgtc cttctggttc cgtgactttg tctttatgcg
    16381 aatggtgatg gtgttaacca gaaagaaagt ctttaaaaat cgtaatgtaa cctcaagcat
    16441 ggcctacatt gtaaatatgc tgattatggg attttggcat ggtgtgacct ggtactatat
    16501 cgcctatgga ctctttcatg gactaggctt ggtcatcaat gatgcctggg ttcgcaagaa
    16561 aaaaacgctc aataaggaac ggaaaaaagc agggaaggct gccctacctg agaatcgctg
    16621 gattcagttg cttggcatgg ttgtcacttt ccatgttgtc atgttgtcat tcttaatctt
    16681 ttctggattc ttgaataatc tatggtttaa aaaataaaag gaaataaaat atggatatca
    16741 aatcagaagt tattgaaatt attgatgagt tgtttatgga agatgtttct gacatgatgg
    16801 atgaagatct ttttgatgca ggtgtcttgg atagtatggg aacggttgag ttgattgtgg
    16861 agattgagaa ccgttttgac attcgtgtcc ctgtaacaga gtttggtcgt gacgactgga
    16921 atacagctaa taaaatcata gctggtattg tggagctaca aaatgcttaa acgcttatgg
    16981 atgatcttcg gaccggtctt gatcgctggt ttgttggttt ttctgctcat tttcttttat
    17041 cctactgaga tgcatcataa tctaggagct gaaaagcgtt cagcagtggc tactactatc
    17101 gatagtttta aggagcgaag tcaaaaagtc agagcactat ctgatccaaa tgtgcgtttt
    17161 gttcccttct ttggctctag tgaatggctt cgttttgacg gtgctcatcc tgcggtatta
    17221 gctgagaaat acaatcgttc ctaccgtcct tatcttttag gacagggggg agctgcatcg
    17281 cttaaccaat attttggaat gcaacagatg ttaccacagc tggagaataa acaagttgtg
    17341 tatgttatct cacctcagtg gttcagtaaa aatggctatg atccagcagc cttccagcag
    17401 tattttaatg gagaccagtt gactagtttt ctgaaacatc aatctgggga tcaggctagt
    17461 caatatgcag cgactcgctt actgcaacag ttcccaaacg tagctatgaa ggacctggtt
    17521 cagaagttgg caagtaaaga agaattgtcg acagcagaca atgaaatgat tgaattattg
    17581 gctcgtttta atgaacgcca agcctccttt tttggtcagt tttcggttag aggctatgtt
    17641 aactacgata agcatgtagc taagtattta aaaatcttgc cagaccagtt ttcttatcaa
    17701 gcaatagaag atgttgtcaa agcagatgct gaaaaaaata cttccaataa tgagatggga
    17761 atggaaaatt atttctataa tgagcagatc aagaaggatt tgaagaaatt aaaggattct
    17821 cagaaaagct ttacctatct caagtcgcca gagtataatg acttgcagtt ggttttaaca
    17881 cagttttcta aatctaaggt aaacccgatt tttatcattc cacctgttaa taaaaaatgg
    17941 atggactatg ctggtctacg agaggatatg taccaacaaa cggtgcagaa gattcgctac
    18001 cagttagaaa gtcaaggttt taccaatata gcagattttt ctaaggacgg cggggagcct
    18061 ttctttatga aggacaccat tcaccttggt tggttgggtt ggttggcttt tgacaaggca
    18121 gttgatcctt tcctatccaa tcccacacca gctccgactt accatctgaa tgagcgcttt
    18181 ttcagcaaag attgggcgac ttatgatgga gatgtcaaag aatttcaata gataatataa
    18241 tatagaagag ttcaagaatg aaactagttt tcacgttttt tcttgactct ttttttgctt
    18301 gtgatataat taactggtaa acaaaatgtc aaagaatagt atttttctct taaaaaagag
    18361 aagtctaaaa ggaaaggagt cagatatgag acagctagca aaggatatca atgctttttt
    18421 gaatgaggtg attttgcagg cggaaaatca gcatgaaatc ctaataggtc attgcactag
    18481 cgaggtggcc ctgaccaata ctcaggagca tatccttatg ctcttgtcag aggaatcttt
    18541 aacaaattca gaattggccc gtcgtctcaa tgtcagtcag gcggcagtta ccaaggccat
    18601 taagtctttg gtcaaggaag ggatgttgga aacatctaaa gattctaaag atgcgcgtgt
    18661 gattttttat cagttgactg acttggctcg tccaatcgct gaggagcacc accatcacca
    18721 tgagcataca cttttaacct atgaacaagt ggcgactcag tttactccaa atgaacaaaa
    18781 agtgattcag cggtttttga ctgctttagt aggagaaatc aaataatgag atatattacg
    18841 gtagaggatt tgtccttcta ttatgataag gagcctgttc ttgaacatat caattattgt
    18901 gttgatagtg gggaatttgt taccttgact ggggaaaatg gagcggctaa gacgacgctc
    18961 atcaaggcta gtcttggaat tctgcaacca cgcattggaa aggtggctat ttcaaagaca
    19021 aatacgcaag gtaagaaatt gagaatagcc tatcttcctc aacaaattgc cagttttaat
    19081 gctggttttc caagtacggt ctatgaattt gtcaagtcgg gtcgctatcc gagaaaaggc
    19141 tggttccgtc gtttgaatgc tcatgatgag gagcatatca aggccagtct ggactcagtt
    19201 ggcatgtggg aacatcgaga caaacgcttg gggtctctat ctgggggaca aaagcagcga
    19261 gcggtaattg cgcgtatgtt tgcttctgac cctgatgtgt ttatcctaga cgagccgaca
    19321 acggggatgg atgcaggaag taaaaatgaa ttttacgaac tcatgcacca cagcgcccat
    19381 catcatggca aggctgtttt gatgattacc catgaccctg aagaagttaa ggattatgcg
    19441 gatcgcaata ttcatctagt ccgtaaccaa gactcgccat ggcgttgttt caacgttcat
    19501 gagaatggcc aggaggtggg ccatgcttag tttattatct tacgacttta tacaacgcgc
    19561 ctttttggcg gttattgcta tgagtctttt ctcaccggta ttgggaacct tcctcatctt
    19621 gcgtcgtcag agtttgatga gtgataccct tagccacgtc tcactttcag gtgtagcctt
    19681 tggtctggtt ttggggattt ctccaactgt ttctactatt gccattgtct tgattgcggc
    19741 ggtctttctg gagtatctcc gtacggttta caagagcttt atggaaatcg ggacagctat
    19801 cctcatgtca acaggtctgg ctgtttctct gattgtcatg agcaagggta aaagctcgag
    19861 ttcaatgagt ttggaccaat atctctttgg ttcgatcgtg actatcagtg aagaacaggt
    19921 catttccctc tttgtcattg cggcggttgt tttgattttg acctttctct ttcttcgtcc
    19981 tatgtatatc ttaacttttg acgaagatac ggcctttgtg gatggcttgc cagttcgtac
    20041 catgtccatt ctttttaaca tggtgacagg ggtggctatt gcccttatga ttcctgcagc
    20101 aggagctctt ctggtatcga ccattatggt cttgccagct agtattgccc tgcgtctggg
    20161 gaaaaacttt aaatcggtta tgctgcttgc cagtgcgatt ggctttttgg gaatggtagc
    20221 aggactttac atttcctact atgcagaaac acctgcaagt gcaagtatta ccattatttt
    20281 tgtaactgtc tttatactaa tcagtttagt aagacgtttt atcaaatagg agacgaacgt
    20341 gaaaaaaatt agcttattac tagccagtct atgtgccttg tttttagtgg cttgttccaa
    20401 tcaaaaacag gcagatggta aactcaatat cgtgacaacc ttttaccctg tctatgaatt
    20461 taccaagcaa gtcgcaggag atacggctaa tgtagaactc ctaatcggtg ctgggacaga
    20521 acctcatgaa tacgaaccat ctgccaaggc agttgccaaa atccaagatg cagatacctt
    20581 tgtttatgaa aatgaaaaca tggaaacatg ggtacctaaa ttgctagata ccttggataa
    20641 gaaaaaagtg aaaaccatca aggcgacagg cgatatgttg ctcttgccag gtggcgagga
    20701 agaagaggga gatcatgacc atggagaaga aggtcatcac catgagtttg acccccatgt
    20761 ttggttatca ccagttcgtg ccattaaact agtagagcac atccgcgaca gcttgtcagc
    20821 agattatcct gataaaaaag agacctttga gaagaatgca gctgcctata tcgaaaaatt
    20881 gcaagccttg gataaggctt acgcagaagg cttgtctcaa gcaaaacaaa agagctttgt
    20941 gactcaacac gcagccttta actatcttgc cttggactat ggactcaaac aagtcgcaat
    21001 ctcaggactt tctccagatg cagagccatc agctgctcgc ttggcagaat tgacagagta
    21061 tgtcaagaaa aataaaatcg cctatatcta ttttgaagaa aatgcctcac aagcccttgc
    21121 taacacactt tcaaaagaag caggtgtcaa aactgatgtc ctcaatcctt tagaaagtct
    21181 gacagaagag gacaccaagg ctggagaaaa ctacatttcc gtgatggaga aaaacctcaa
    21241 ggctttgaaa caaacaacag accaagaagg cccagcaatc gaacctgaaa aggcagagga
    21301 taccaagaca gtccaaaatg gttacttcga ggatgcagct gtcaaggacc gtaccttgag
    21361 tgactatgca ggtaactggc aatcagttta tcctttcctt gaagacggca cgtttgacca
    21421 agtctttgac tacaaggcta agttaactgg taagatgacc caggctgagt acaaggctta
    21481 ctatacaaaa ggctatcaga cagatgtgac taagattaac attactgata atactatgga
    21541 atttgttcaa ggtggacaaa gcaagaaata cacttacaag tatgtcggta agaaaatttt
    21601 gacttacaag aaaggcaatc gtggcgtgcg tttcctcttt gaagccacag atgctgacgc
    21661 tggacaattc aagtatgttc agtttagtga ccacaatgtt gccccagtta aggcagaaca
    21721 tttccatatc ttctttggag gcacaagcca agaagccctc tttgaagaaa tggacaactg
    21781 gccaacctac tacccagata acctatctgg ccaagaaatc gcccaagaaa tgttggcgca
    21841 ttgatgagaa atcgactagt tcataagagc tagtcggttt tttgatacta gatggctaac
    21901 ttcattatag acctttcaga actaagggaa cctgaaaaaa taaataaaac tcttgaaata
    21961 tctgggctta tatgctagaa tgtagttagt gtataaggtt cattagaaca ccaaagcccc
    22021 taactagtgc catagttaag ggcttttttg acgtatctta gcgatttaat gggtgtttat
    22081 aggctagtca gtcggtctac ttcttaccat ctagccattt gcagacgaaa tacaagataa
    22141 ttccagctat aacatctgct ataatagtaa gagcaagctc tggtataagg ctcattagtt
    22201 tcacctcctc tcacgaaccc ataggaacgt aatcggtaac cgatgacaaa aatagtatac
    22261 cacactaaat ttagatcatc aacctcactt aatgcttgaa ctattgtaga ttatatgcta
    22321 aaatgtagtt aattgataag gttcattaga acaccaaagc ccctaactat ggccgtagtt
    22381 aagggctttt ttgacgtatc ttagcgattt aacgggtgtt tataggctag tcagtcggtc
    22441 tactccttac tatctagcca tctgcagacg aaatacaaga taattccagc tataacatcc
    22501 gctataatag taagagcgag ctctatgata aggctcatta gtttcacctc ctctcacgaa
    22561 cccataggaa cgtaatcggt aaccgatgac aaaaatagta taccacaata aatttagatc
    22621 atcaaggtca cttaattctt gaaatatagg gtctaggcga caaagtcttt aaaatcagaa
    22681 aaacgcataa tatcaggtgt gcaaaaactt gatactatgc gttttattgt gggaaggttt
    22741 actccatttt ctcctgaaat tgagtttttg tccagcctct gtttttgggg ttgctaagaa
    22801 aataatgtca tgtggtgaat atttgtaaat cagtcagcag acagaacgaa tactcttcga
    22861 aaatctcttc acatcatgtc agcttcgtct ttccgtatat atgtgactga cttcatcagt
    22921 tctatctaca acctcaaaac agtgtttcga gctgacttca tcagttttca aatctgtact
    22981 ttgagcaagc tgagactagc ttcctatttg attttcattg aatatcagaa acccattctc
    23041 catcaaataa ttcgactgcg tctaataatt tttgatctgg cacggtgtct gaaataaagg
    23101 ttgtgtattt ggagagggga ttaattttaa aaaatccagt cttgtaaaat ttagaactat
    23161 caatcagtaa gatggtttca tgggctttgt caataatatt cttttttgaa atagcttggc
    23221 tgagagaagc ttcataaaca tattggtcat caatacctct tgctgaacaa aatgctaaat
    23281 cgatattaaa atgatctaat aaagaatttt ccttatcata gttgaccacg gaacaggatt
    23341 gatgtttgac ctcgccagat gtgataaaga ttttggagct atctttaaca gtttcagata
    23401 gggtttgtgc agtatgtaaa ccatttgtaa aaataatcaa attatcaagt tcagaaagat
    23461 agggacagag ttcgtagaca gtagtactgg aatccagata gatacacata ccagaccgaa
    23521 taaagtcttt agcgagacta gcgattagtc ttttttgcct agtactttct ccttcacgta
    23581 tttgatgaga aagttcaatt gtgttcatag aggacagggt cacgtatccg tgctttcttt
    23641 tgataagacc ttgattttct aagaaaatta aatcacgacg taaggtactt gtgctggaga
    23701 aagtgatttc tgccagctct tttacggcaa ttcttttttt ctttttgata atttcaatca
    23761 attcaagtac acgttcatct tttatcataa gctcctccta atttatcatt tcaactatat
    23821 tatagcacaa attggaggaa tttgaattat ttttatgaat attgggttaa catttgaaca
    23881 ttatccaagt aagcgttcac atattgaaaa aataaaatgt ggggattata ataaacttaa
    23941 tcaaggacga agagagaaga aaaatggaag cggttttagc aatagattta ggtgcgactt
    24001 ctggaagagc aatcgttggt tacctttctg aaaataaact agtaatggaa gaaataaatc
    24061 gcttttctaa tctacctatt agagtaaaag ggcatttatc ttgggatgtt gactttctac
    24121 tagctaaaat tcttgaaagt atccgcttgg ctaatactag ttacaagatt ttatctatcg
    24181 gtattgacac atggggagtt gattttggcc tgattgataa tgaaggtaag ctgttatcac
    24241 aacctgttca ttatcgtgat gaaagaacaa agggagtgtt aaaggaaata tctgaaatga
    24301 ctgaattaga aaaactgtat tcagagacag gaaatcagat tatggagata aataccttgt
    24361 ttcaactctt taaggcacgt caagaatctc ctgactcttt ctataagacc aataagattc
    24421 ttttaatgcc agatttgttt aattatctct tgacaggtaa gtttgctaca gaaaaaagca
    24481 ttgcttcaac aactcaatta tttgatccta ggagtcaaaa ttggaatcag aatatcttaa
    24541 aactatttga attgaattca tctttacttc ctgaaattgt ttcagaggga aatgttcttg
    24601 gaaggataaa aaaggagtat ggtttaggcg atattcctgt tgtgaatgtt tgtagtcatg
    24661 atacagcaag cgcgattgtc tcagtaccta agacagaagg tagtttattt atttcatcag
    24721 gtacttggtc tttggttgga gtggaactta cttcaccgat tcttactacc gaatccttca
    24781 gttatggatt tacaaatgaa gtcggtaaag atggagtgat tacatttctg aagaattgta
    24841 cagggttgtg gatcatagag gaactaagac gttcatttga acgaagaggg aaagcctatt
    24901 cttttgatga tattaggaca atggtggaga aagaaaaaga aaatcttcct ctgattgata
    24961 ctgaatcaac tgaatttgca acagaatctg atatgcacaa gactttgaca gaatatctag
    25021 cttatcatca tgaaactaga gagtggacag atggacaact atttaagatt gtttatgaaa
    25081 gcctagctga aacgtatagg aaagcgatag agttactaga agaactaact cataaggttt
    25141 ataagaggat atatgtgatt ggaggaggtg ctagagccag ttattttaac caaatgattg
    25201 ctgatagaac tggtaaagag gttcttacag gttcgactga gggtacagct gtggggaata
    25261 ttgttgtgca gctcatagct atgggacaat taaaagggat ggaagaagct caccatgtta
    25321 ttgaggagtt tctacaatta gagagttatt actcccaaaa gaattaaaaa gattgagagt
    25381 ttgtaaattt gcctccctcc cccttcttaa gttttgtaca agaagggggg ataattggtg
    25441 aattgaaaaa tatttagtgt tttgatatga ggaggacaag aatgtcagat gtaaaacaag
    25501 aattaattaa gtatggcaag aagctagtag aaacagattt gacgaaagga acaggtggga
    25561 atctcagtgt tttcgatcgt gaaaaacaat tgatggcaat taccccgtcg ggtattgatt
    25621 tctttgaaat caaagaatcc gatattgtag tgatggatat taatggaaat gttgtagagg
    25681 gagaacgctt gccatctagc gaatggtata tgcatttgat tcaatatcaa actcgtgatg
    25741 atatcgatgc aattatccat gctcatacaa cttatgcaac agtattagct tgtctcagag
    25801 aaccacttcc ggctagtcat tatatgattg cagtggcagg gaaagatgtt cgggtagctg
    25861 agtatgcaac atatggtacg aaagaattgg ctgtgaatgc agctaaagca atggaaggtc
    25921 gtagagcagt tttactagcg aatcatggaa ttttagcagg tgcacaaaat ttattgaatg
    25981 catttaatat tgttgaagaa gttgaatatt gtgcaaaaat ttattgttta gctaagaatt
    26041 ttggagagcc agtagttctt cctgatgagg agatggattt aatggcagaa aaatttaaaa
    26101 catacggtca gagaaaatag ggaggatatt aatgttaaaa catataccga aaaatatttc
    26161 tccagattta ttgaagactt taatggaaat gggacatgga gatgaaatag tattagctga
    26221 cgcgaattat ccttctgcct catgtgcaaa taagctaatt cgttgtgatg gtgtaaatat
    26281 tccagaatta ttagattcca ttctgtattt aatgccatta gatagttacg tcgatagttc
    26341 aattcagttt atgaacgttg tttcgggtga tgatattcct aagatatggg gtacctatag
    26401 acagatgatt gaaggtcatg gtacagatct taaaacgatt acttatctta gaagagaaga
    26461 cttttatgaa cgtagtaaga aagcttatgc tattgttgct acaggagaaa cttcacttta
    26521 tgctaatatt atccttaaga aaggagtagt tgttgaaaga gaaaatgttt aatagaggaa
    26581 ttttagttgc cagtcatggt aattttgcta gcggagctct catgaccgca gaaatgtttg
    26641 ttggtgagac aacaaatgat agagttagga cattaggttt gatgcctgga gagaatattg
    26701 tagagtttga acattatttt aaaaatcaag taaatgaact gttagactca aatcaagagg
    26761 ttatcgtttt gactgacttg attggaggaa gtcctaataa tgttgctttg tcacggtttt
    26821 taaatttgga ttcagttgat attgtaacag ggtttaatat ccctctccta gtggaattaa
    26881 tatcaaatta tgattcaaaa atcaatttag aagaaattgt tcacaatgct caaaatagtt
    26941 tgtttaatgt taaacaacaa cttaacgtag aggaggaaga agatttatgt ctatagagtt
    27001 tgttcgtatt gatgaccgtc tggtacatgg tcaagttgtc actacgtggc taaaaaagta
    27061 tgatattgag caagttatca ttgttaatga tcgcatctca gaagataaaa cacgacaatc
    27121 tattttaaag atttctgcac cagtaggttt aaaaattgtt ttctttagtg taaaacggtt
    27181 tgtggaagtt ttaaactctg tgccaataaa aaagagaaca atgctgatat atacaaatcc
    27241 aaaagatgtg tatgattcta ttgaaggaaa tttaaaattg gagtacctca atgtaggaca
    27301 gatgagtaaa acggaggaaa atgaaaaggt aacgggaggt gtagctctag gtgaagaaga
    27361 caaatattat tttaagaaaa tagttgataa gggaacgaga gttgaaattc aaatggttcc
    27421 taatgataaa gttacaatgt tggaaaaatt tttataaaaa taatttaagg aggtacagta
    27481 tatgctattc acacaagcat tactggtgac attagttggg attattgcca ctattgacta
    27541 taatggaccg ttatttatga ttcaccgtcc gttagttaca agtgcaatgg ttggcttagt
    27601 cttaggagat ttcacccaag gtgttcttat tggttcagct cttgaattaa cttggctcgg
    27661 tgtaacaggt attggaggtt acactccacc agatactatt tcaggtgcga ttattggtac
    27721 tgcatttggt attttatctg gtcaaggaga aactgctggt atcgctatag cagttccaat
    27781 tgcagttgct acccaacagt tggatgttct tgcaaaaact ttagatgttt attttgtgaa
    27841 aaaagctgat aatgatgcta aaaacggaga ttattcaaag atcggttttt atcattattc
    27901 aagtttggtt ttaatcacgt tatttaaaat tgtaccaatt ttcctagcta ttatgcttgg
    27961 aggggaatat gtggcagact tgtttgcgaa ggttccacca atcgttatgc agggacttaa
    28021 ctctgcaggt gctttactac cttcaattgg ttttggtatg cttttaaata tgatgctcaa
    28081 gaaaaatatg tgggtattct tgttgattgg attcatttgt tctgtgtatg gaggaatgtc
    28141 aaccattggg atctcactag ttggtattgc ggtagcatac ttctacgata tgattggaag
    28201 taaaccacaa gaaacaactt caagtagtga tgttgaggag gatcttgatc tatgatgaat
    28261 aataaagtaa ctaaagttga acttaaaaaa gttttcaaac gaagttttat gtatggttct
    28321 tcatggaact atgagagaat gcagaaccta ggttttctat atacaattct tccagtattg
    28381 aaaaaactat acccagacaa agattcagct tctcctgcaa tgaaacgtca ccttgagttt
    28441 ttcaatactc atcaaacagc ggcaccattt attcttggag ttacttccgc tatggaagaa
    28501 caagaaggaa atgaaggtgc agcttcaatt actggtatta aagttggctt gatggggcca
    28561 ctggctggtc taggagatag tttgttctgg ctgacactag ttcctatctg ttttagtatt
    28621 ggtgcgtctt attctaaaga cggcggtgct ttaggtatct ttatcgcctt aatattgttt
    28681 aatattatta atattcctgt taaatatttc ggtttgaaat atgggtatac taagggttct
    28741 agtcttatcc aagaaaataa tacaaaagga acattgaatc gcgttacgag tatggcgaca
    28801 gcattagggc tagtactagt gggtggtttg attccatcaa tggttggtat taattttgga
    28861 ttagaattta agcaggggga acttgttatt tctgttcaag aaatgattac aaaattaatt
    28921 ccaggattta tccctatggc tttgacttta ttaatgtgta aattaattag aaaaggaaag
    28981 aatccggttg tactaatctt tagtgttatg gctattggag ttatcctagt tgttttagga
    29041 attttgaagt agtagaaagt gtggaggtgg tatttgggat atcacctcca ttttggaaga
    29101 gaggtaaaga gtgaaattat ggtataagaa agctgcctca aattggaatg aagccttgcc
    29161 gattgggaac ggtcatttag gtggtatgat ttatggttca gctacaaaag aatgtattca
    29221 actaaacgat gagactattt ggtatagagg aaagtcagat agaaataatc cagactcact
    29281 attgcatctt aaaaaaattc gggaatatct tttagatgga gaaattcaga aagccgaaga
    29341 attgataaag ttaacaatgt ttgctacccc aagagatcaa agccactatg aattacttgg
    29401 ggaactttac attgagcata tagatattca gtcttgtgct ctttcattgt atgaaagaga
    29461 gctagattta gatacagcta tttctaatgt tgtgtttgag cctaatagtt gtaatttaca
    29521 aataaaaaga gaatatttta cgagttttaa taagaatatt ttatgttgcc gtatagtgtc
    29581 atcagttcaa aacacattaa atttaaacat taatttgggt agaaataaac ggtttaatga
    29641 tgaagtatct aaactggatt caagtacaat tttaatgtcg gcctctgctg gaggtagaaa
    29701 aggtgttcag tttaaagtag tatgtcattc taaggttacg gatggtgaag taagtgtatt
    29761 gggagagaca atagttattc ggaatgctac agaggtattt ctttatctca aatcaatgac
    29821 ggattattgg ggaaatatag atatttcttc tcttcaggga gaatttagta gtattgatta
    29881 ctttacagaa aaagatgaac atgtaaaaaa atatcaggag caatttaata gagttgattt
    29941 taaactagac tatagtaaag attgtcttag cattccaacg aatctacttc ttgaaaacac
    30001 taaaaagtat agtaactact tgactaactt gttatttcat tatggaagat atctgttaat
    30061 atcgtctagt caaccgaatg gtttacctgc caatcttcaa ggaatatggt gtgatgaatt
    30121 aaatccaatt tggggttcta aatatacgat taatattaat actcaaatga attattggat
    30181 ggtaggtcca tgtgatttac cagaagtaga atatccatta tttgatatgc tcgaaagaat
    30241 gagagaaccg ggaagactaa ccgctaagaa aatgtatgga gctagaggtt ttacagcaca
    30301 tcataatacg gatggttttg gcgatacggc tccccaatct catgccatgg gggctgcaat
    30361 ttgggtatta actattccat ggttatgtac tcatatttgg gaacactatt tatatttcca
    30421 agatgagcgt attcttacgg aacattttga aatgataaaa gaagcatttc ttttctttga
    30481 agattattta tttgaggtgg atggctactt gatgacaggt ccaagtgtct caccggaaaa
    30541 taaatatcgc ttaaaaaatg gtattgaagg aaatgcttgt ctatcatcta caattgataa
    30601 tcaaatttta agatattttt gtgattcatg cattggaatt gcaaaacaat taggagacaa
    30661 ttcggatttt attagtcgtg tgaaggagtt aaaaaagaaa ttacctagaa caaaaatagg
    30721 tagtaatggg caaatacaag aatggctaga ggattatgaa gaagtagagc ctggtcatag
    30781 acacatttca cctctatttg ggctttatcc ttataatgag attgatattc ataaaactcc
    30841 ggaattagca gaagcagcta aaatcactat caatagaaga ttatcaaacg ctaacttttt
    30901 atcttcacag gatagggagc aagcgattaa taattggtta gtaagtggtt tgcatgctag
    30961 tacacaaaca ggttggagtg ctgcatggct gattcatttt tttgcgagac tatatcaagg
    31021 tgaacctgct tataaccaga ttaatggttt gttaaataat gcgactcttg gcaatttatt
    31081 tcttgaccat ccaccatttc aaattgatgg taatttaggt ttggtgagtg gaatttgtga
    31141 attattagta cagagccatc ataattggtt atcactaatt ccagctttac cttctgcttg
    31201 gtcagaagga gaagtgaaag gtttcagagt aagaggagga tataaggtat cgtttgcttg
    31261 gaaaaatggg gatataacat tcctaaaatt ggaaggagga aacaaagatc aaaaagtaag
    31321 agtaagagta tatggcaaaa atactgatgt acaaaatatt gaattggtat ttaattcaga
    31381 aaaaattatt gagttaaatt tttaggtata agtcatgaat aaagaaaaaa taaaaagaaa
    31441 attaatcaca atactgtttg tatgtattgg gatgttatgt tttggattgt tagcaggagt
    31501 taaggctgat aatcgtgttc aaatgagaac gacgattaat aatgaatcgc cattgttgct
    31561 ttctccgttg tatggcaatg ataatggtaa cggattatgg tgggggaaca cattgaaggg
    31621 agcatgggaa gctattcctg aagatgtaaa accatatgca gcgattgaac ttcatcctgc
    31681 aaaagtctgt aaaccaacaa gttgtattcc acgagatacg aaagaattga gagaatggta
    31741 tgtcaagatg ttggaggaag ctcaaagtct aaacattcca gttttcttgg ttattatgtc
    31801 ggctggagag cgtaatacag ttcctccaga gtggttagat gaacaattcc aaaagtatag
    31861 tgtgttaaaa ggtgttttaa atattgagaa ttattggatt tacaataacc agttagctcc
    31921 gcatagtgct aaatatttgg aagtttgtgc caaatatgga gcgcatttta tctggcatga
    31981 tcatgaaaaa tggttctggg aaactattat gaatgatccg acattctttg aagcgagtca
    32041 aaaatatcat aaaaatttgg tgttggcaac taaaaatacg ccaataagag atgatgcggg
    32101 tacagattct atcgttagtg gattttggtt gagtggctta tgtgataact ggggctcatc
    32161 aacagataca tggaaatggt gggaaaaaca ttatacaaac acatttgaaa ctggaagagc
    32221 tagggatatg agatcctatg catcggaacc agaatcaatg attgctatgg aaatgatgaa
    32281 tgtatatact gggggaggca cagtttataa tttcgaatgt gccgcgtata catttatgac
    32341 aaatgatgta ccaactccag catttactaa aggtattatt cctttcttta gacatgctat
    32401 acaaaatcca gctccaagta aggaagaagt tgtaaataga acaaaagctg tattttggaa
    32461 tggagaaggt aggattagtt cattaaacgg attttatcaa ggactttatt cgaatgatga
    32521 aacaatgcct ttatataata atgggagata tcatattctt cctgtaatac atgagaaaat
    32581 tgataaggaa aagatttcat ctatattccc taatgcaaaa attttgacta aaaatagtga
    32641 ggaattgtct agtaaagtca actatttaaa ctcgctttat ccaaaacttt atgaaggaga
    32701 tgggtatgct cagcgtgtag gtaattcctg gtatatttat aatagtaatg ctaatatcaa
    32761 taaaaatcag caagtaatgt tgcctatgta tactaataat acaaagtcgt tatcgttaga
    32821 tttgacgcca catacttacg ctgttgttaa agaaaatcca aataatttac atattttatt
    32881 gaataattac aggacagata agacagctat gtgggcatta tcaggaaatt ttgatgcatc
    32941 aaaaagttgg aagaaagaag aattagagtt agcgaactgg ataagcaaaa attattccat
    33001 caatcctgta gataatgact ttaggacaac aacacttaca ttaaaagggc atactggtca
    33061 taaacctcag ataaatataa gtggcgataa aaatcattat acttatacag aaaattggga
    33121 tgagaatacc catgtttata ccattacggt taatcataat ggaatggtag agatgtctat
    33181 aaatactgag gggacaggtc cagtctcttt cccaacacca gataaattta atgatggtaa
    33241 tttgaatata gcatatgcaa aaccaacaac acaaagttct gtagattaca atggagaccc
    33301 taatagagct gtggatggta acagaaatgg taattttaac tctggttcgg taacacacac
    33361 tagggcagat aatccctctt ggtgggaagt cgatttgaaa aaaatggata aagttgggct
    33421 tgttaaaatt tataatcgca cagatgctga gactcaacgt ctatctaatt ttgatgtgat
    33481 tctatatgac aataatagaa acgaagttgc taagaaacat gttaataatt tgtcgggtga
    33541 atctgttagt ctagatttca aagaaaaagg agcaaggtat attaaagtta aattactaac
    33601 gagtggagtg cctttgagtt tagcagaagt agaggttttt agagaatcag atggtaagca
    33661 atctgaagag gatatagata aaataacaga agataaagta gtctctacaa ataaggtagc
    33721 tactcaaagt tcaaccaatt atgagggtgt agctgcttta gcagttgatg gtaataaaga
    33781 tggagattac ggacatcatt cggtgactca tactaaggca gattctaacg cttggtggca
    33841 ggtcgatctg ggagaagagt ttacggtttc taaagttgat atttataata gaacagatgc
    33901 cgaacctcag cgtttatcta attttgatgt tatttttcta tcttcatcag gagaagaagt
    33961 ttttagaaga cattttgata aagtagttga tggtttgtta tctttaaaag taccttctgt
    34021 aggggctaag ctagtcaaaa tagaattaaa atcagcagct attccgttaa gtttagcgga
    34081 agttgaagtc tatggttcaa agagaactcc gaagaaactt tctaatattg cattaaccaa
    34141 agaaactcga cagagttcaa cggattacaa tggtttttct cgtctagcag ttgatggaaa
    34201 taaaaacgga gattatggtc atcattcagt gactcatacc aaaggagatt ctccttcatg
    34261 gtgggagata gatttagcac aaaccgaaga attagaaaag ttaattattt ataatagaac
    34321 agatgctgaa attcagagat tatcaaattt tgatattatt atatatgatt caaataatca
    34381 tgaagttttt aaacaacata ttgacagttt agaaagcaat aatctatcca tagacttaaa
    34441 agggctgaag ggaaaaaagg ttagaatttc tttgagaaac gcaggaattc ctttaagttt
    34501 ggcagaggta gaggtttata cttataagta attttaaaaa ttatcatcgg tagtatgaaa
    34561 gaaacagaaa aataagagga aaatagtatg attcaacatc cacgtattgg gattcgtccg
    34621 actattgatg gtcgtcgtca aggtgtacgc gaatcacttg aagtacaaac aatgaacatg
    34681 gctaaaagtg tggcagattt gatttcaagc acattgaaat atccagatgg ggaacctgtg
    34741 gaatgtgtga tttctccatc taccattggt cgtgttccag aggctgcagc ttcccatgag
    34801 ttgtttaaaa aatcaaatgt ttgcgcaaca attacagtta caccatgctg gtgttatggt
    34861 agtgaaacta tggatatgtc tccagatatt cctcatgcta tttggggatt taatgggaca
    34921 gaacgcccag gagctgtcta tcttgcagct gtactagctt cacatgctca aaaagggatt
    34981 ccagcctttg ggatttatgg tagagatgtt caggaagcta atgatacagc tattccagaa
    35041 gatgtcaaag aaaaactttt acgttatgcg cgggcagctc ttgcaactgg cttgatgaga
    35101 gatacagctt acttatcaat ggggagtgtt tcgatgggga ttggtggttc tattgtaaat
    35161 ccggatttct tccaagaata cctaggaatg cgaaatgaat cggtagatat gacggagttc
    35221 acacgccgta tggaccgtgg tatttacgac cctgaagagt tcgaacgtgc gctcaaatgg
    35281 gtgaaagaaa acgtaaaaga aggattcgac cataaccgtg aagaccttgt tttaagccgt
    35341 gaagaaaaag atagacaatg ggaatttgtt attaagatgt tcatgattgg acgtgactta
    35401 atggttggta acccaagact tgctgaactt ggttttgagg aagaagcagt tggtcaccat
    35461 gctttagtag ctggtttcca aggtcaacgt cagtggacag accatttccc aaatggggac
    35521 tttatggaaa ctttcctcaa tactcagttt gactggaatg gtattcgaaa accatttgta
    35581 tttgcgacag agaatgattc actaaatggt gtgtctatgc tctttaatta tctattaaca
    35641 aatactccac aaatctttgc tgatgtgcgc acttattgga gtccagaggc tgttaaacgt
    35701 gtaacgggac atactttaga gggttgtgct gcagctggct tcttacatct aatcaactct
    35761 ggttcttgta cattggatgg tacagggcaa gctactcgag atggcaaacc tgttatgaaa
    35821 ccattctggg agttggaaga aagtgaagtg caggctatgc ttgaaaatac agacttccca
    35881 ccagcaaacc gcgaatactt ccgtggagga ggattctcaa ctcgtttctt gacgaagggg
    35941 gatatgccag taacaatggt acgtctcaat cttctaaaag gggttggtcc agtgctacaa
    36001 attgcagaag gttatacact tgaacttcct gaagatgttc accatacttt agataatcgt
    36061 acagatccag gatggccaac tacttggttt gctccacgtt tgacaggaaa aggtgctttc
    36121 aagtctgtct atgacgtcat gaataattgg ggagctaatc acggagccat aacatatgga
    36181 cacattggag cagacttgat taccttggct tctatgttga gaattcctgt caatatgcat
    36241 aatgtacccg aggaagatat ctttagacct aaaaattggt ccttatttgg aacagaagat
    36301 ctagaatcag cagactatcg tgcatgtcag ttgttgggac cgctacataa ataaaaattg
    36361 tgtatatagg aggtgaactt atgtccctcc tatcctttta aaaagatttg ttaaacaatt
    36421 cacaaataat tgaaaacgaa tacaaaaact aatataatga tgttaaatag atagcgcgga
    36481 ggcgcaggag gaaaattata tggctacatt ttatgttccg gcagtcaacc ttattggtaa
    36541 aggtgttgta aatgaagtgg gtccttatat caaggaactt ggctataaaa aggcactttt
    36601 ggtgacagat aagtacatcg aaggcagtga cattttacct aaggttttaa aaccactgga
    36661 tacagaagga atcgaatatg tcatctttag cgatgtagag ccaaacccta cttgtaaaaa
    36721 tgtcacagat ggggtagctg ctttgcaaga acatggctgt gactttatca tcagtcttgg
    36781 cgggggttct ccacaggatg cagctagttg tatttctatc atggctacaa atggtggaaa
    36841 accacaggat tatgaagggc ttcataagtc tgctaaaaaa ggcttgccag ttgtggctat
    36901 caatacaacg gcaggaactt ctgcagaaat cactattaac tatgtgatta ctgatgaaga
    36961 acgcaaggtt aagatggtaa tggttgacaa gaatagcctt gctcttatct ctgttaatga
    37021 cccggaactc atgctttcca aacctaaagg cctgactgct gctactggta tggatgctct
    37081 gactcacgct gttgaagctt tggtaacacc tggtgcttat gatgtaacca agaaactgtc
    37141 tattggtgct attgagctta tcaaggaata tcttcctcgt gctgtagaaa atggacatga
    37201 tattgaagcg cgtgaaggta tggtcaatgc catcttcctt ggtggtatga gctttaataa
    37261 tgctggtctt ggttatgttc actcaatggc tcaccaactc ggtgcagtat ataatttgcc
    37321 acatggtgtt tgctgtgcca tgttgctacc agttatagaa cgtgaaaatg ctaaacgtgt
    37381 accagaagct ttccgcaatg ttgccaaagc cttgggactt catgtagaag gtaaatcaga
    37441 tcaagaatgt gccgattatg cgattgttga gattgagaag ctttctgaga cagtaggtat
    37501 tcctaagaaa ctgactgaac ttggtattga agaaaaagat ttcgactttg aatacctttc
    37561 taagaatgcc ttgattgatg cctgcgcacc aggaaatcca tttatgccaa ccttagaaga
    37621 aacgattgcc ttttataaag agttatttta gcaagagatt ggacgacaat aggacaattc
    37681 acctctaatt attgacattt caggaaaaat cgcagtaaaa acggcacagc cttctttaaa
    37741 tgtgctataa tactagaaaa atacttgtgg aggttccatt atggcaatat ttttcatgat
    37801 ttttctgatt gtttgtgtgc tcctattggt gatagtcaca ctgagtacag tttatgtggt
    37861 tcgtcagcag tcggtggcga ttattgaacg ctttgggaaa taccaaaagg ttgctaatag
    37921 cggtattcat attcgcttgc cttttgggat tgactcgatt gcagcacgga ttcagttgcg
    37981 cttgttgcaa agtgatattg tggttgagac taagaccaag gacaatgtgt tcgttatgat
    38041 gaatgtagcg actcagtacc gtgtcaacga gcagagcgtg acagatgctt actataaact
    38101 catacgtcca gaatctcaga ttaaatctta tatcgaagat gctcttcgct cttctgttcc
    38161 aaaattaacc ttggatgaat tgtttgagaa aaaagatgag attgcccttg aggttcaaca
    38221 ccaagtagca gaagaaatga ccacttacgg ctacattatc gtgaaaacct tgattaccaa
    38281 agtcgaaccg gatgcagaag ttaagcaatc catgaatgaa atcaatgcgg cgcaacgtaa
    38341 gcgggtcgca gcacaagaat tggcggaagc tgacaagatt aaaattgtca ctgcagctga
    38401 agccgaagca gaaaaagacc gccttcatgg tgtggggatt gcccaacaac gtaaggcgat
    38461 tgtggatgga ttggcagagt ctatcaccga actcaaggaa gccaatgttg gcatgacaga
    38521 agaacaaatc atgtctatcc tcttgaccaa ccagtatttg gataccttga atacctttgc
    38581 ctctaaagga aatcaaacca tctttttacc aaatactcca aatggtgtgg atgatatccg
    38641 aacacaaatc ttgtcagccc ttcgcgctga gaagaaataa tagactaata ctcttcgaaa
    38701 atctcttcaa accacgtcag cgtcgccttg ccgtatagat gttactgact tcgtcagttc
    38761 tatctgcaac ctcaaaacag tgttttgagc tgacttcgtc agtcttatct actacctcaa
    38821 aacggtgttt tgagcaacct gtggctagtt tcctagtttg ctctttgatt ttcattgagt
    38881 acaaagagct tggcaacagg ctctttttgt attgcgttta ttaagaaaaa caaaaaacat
    38941 cgatatacat ttaactaaaa attattgtat gttatctccc ttaattagga atgataaggg
    39001 aataactaga aagatttgtg aatacaaact atttctgata tacaaaatat acagtaataa
    39061 tgaatgatgg gagatgggat gaaagaattt caatttgaga gaaagcagcg tttttctttg
    39121 aggccatatg caataggagc ttgttcggtc ttgctaggaa cgagtttatt ttttgatggt
    39181 atgggtgatc agcctgtaca ggatacagaa acgagttcag cactaatttt aagtcattat
    39241 ttggatgagc aggatttatc tgaaaagctg aaatctgagt tgcaatggtt tgaattagaa
    39301 aacaagcttt tgaacttatg ggagcattag gttactatga aggatttgtt ccttatgttt
    39361 caaatcaata caaaaaccaa gctgaagaag aaggcaaacc gctatctgat aaatatattt
    39421 tcgaaaaaat cttaggaaaa acatatgcag ccttcaaaaa agatcaaatt aatgagcgtg
    39481 tcgagaaatt aggtaagtta aaacctatta caataaatta caacggaaaa tcagaagtaa
    39541 ttgatagtaa agaaaaatta caagagctta tgaataaagc cgttaaagac gaagtggctc
    39601 aaatatagta aaatgaaata agaacaggac aaatcgatca ggacagtcaa atcgatttct
    39661 aaaaatgttt tagaagtaga ggtgtactat tctagtttca atatactata aaatcaggca
    39721 ataccacggc taaaaagttt gagtttatcg aaacaccagt gcaaaaactg aaaaaagcga
    39781 tttataaagc tcatctcaaa gattcagat
//
LOCUS       DAVKQB010000016        38062 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKQB010000016 DAVKQB010000000
VERSION     DAVKQB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38062
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.016"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..137)
                     /locus_tag="VW528_000799"
     CDS             complement(<1..137)
                     /locus_tag="VW528_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9911647.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     G"
     gene            314..1759
                     /gene="cps4A"
                     /locus_tag="VW528_000800"
     CDS             314..1759
                     /gene="cps4A"
                     /locus_tag="VW528_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU9911648.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVITALVLLVALLGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSDIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAVYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNMPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1761..2492
                     /locus_tag="VW528_000801"
     CDS             1761..2492
                     /locus_tag="VW528_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEU9911649.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKVKELFVDNPRKIIMDQLI"
     gene            2501..3193
                     /gene="cpsC"
                     /locus_tag="VW528_000802"
     CDS             2501..3193
                     /gene="cpsC"
                     /locus_tag="VW528_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU9911650.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSMTRIYVVNRNQGEKSGLTNQDLQAGSSLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKDLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPATSPSSPNIKRSTLIGFLAGVIGTSVIVLILELLDTRVKRPKDIEDTLHMT
                     LLGIVPNLNKLK"
     gene            3203..3892
                     /gene="cpsD"
                     /locus_tag="VW528_000803"
     CDS             3203..3892
                     /gene="cpsD"
                     /locus_tag="VW528_000803"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEU9911651.1"
                     /translation="MPTLEIAQKKLEFVKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PEEGKTTTSVNIARSFARAGYKTLLIDGDTRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGTVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTATGEVNKRDVQKAKQQLEQTGKLFLGVVLNKLDISVDKYGVY
                     GFYGNYGKKII"
     gene            3903..5270
                     /locus_tag="VW528_000804"
     CDS             3903..5270
                     /locus_tag="VW528_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9911652.1"
                     /translation="MDEKGLKIFLAVLQSIIVILLVYFLSFVRETELERSSMVILYLL
                     HFFVFYFSSYGNNFFKRGYLVEFNSTIRYIFFFAIAISVLNFFIAERFSISRRGMVYF
                     LTLEGISLYLLNFLVKKYWKHVFFNLKNSKKILLLTVTKNMEKVLDKLLESDELSWKL
                     VAVSVLDKSDFQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFETMG
                     IDVTVNLKAFDKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVIAKRIIDIMGALVGLIL
                     CGLVSIVLVPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGRFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYVQYTSE
                     QKRRLSFKPGITGLWQVSGRSKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFM
                     RDGAK"
     gene            5274..6017
                     /locus_tag="VW528_000805"
     CDS             5274..6017
                     /locus_tag="VW528_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU9911653.1"
                     /translation="MRDRIQLLGVTIDLLTMNETIDIVEQYVLEKRPLHLMGVNADKI
                     NQCHTDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMQCLLELSNKKG
                     YSVYFFGAKEEVLQDMLKVFKRDYPNLIVIGHRNGYFSEEDEQAIQEDIREKNPDFVF
                     IGITSPKKEYIIQKFMDSGVNSVFMGVGGSFDVLSGHIQRAPLWMQKSNLEWLFRVAN
                     EPKRLFKRYFVGNISFIGRVLKAKRGVKY"
     gene            6014..6838
                     /locus_tag="VW528_000806"
     CDS             6014..6838
                     /locus_tag="VW528_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU9911654.1"
                     /translation="MNQTEMIRLIQKVELDAIKEFKKICEENDIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREAYDKLPSVFKDRIIAGKYQVLTYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSVLRKIYFGKVYWYRFLASLGTTYVGDH
                     VDMHSTKQKLIIGFFKKLGFAKLFPQNSVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDTFFKVPTEYDRYLKRLYGENYLYEEPSDDEKKSHLGGH"
     gene            6840..7718
                     /locus_tag="VW528_000807"
     CDS             6840..7718
                     /locus_tag="VW528_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9911655.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNYNAKQIVIIDNFSNNGTG
                     EKLQELYESDLEIDVLINHENAGFARGNNVAYQFAKEKYNPDFMVIMNNDIEIETENF
                     EKIVTDIYREEKFHLLGPDIFSTTYQLHQNPKRLTHYTYGEVKALNEKFKKGSQVSLA
                     LKIKCWLKASKVLRTAIYQNRRKKGSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIRVLHHQNVATNQVYTNLLEKTLFSNKCNF
                     KSTSYFLKLMKENEDV"
     gene            7719..9056
                     /locus_tag="VW528_000808"
     CDS             7719..9056
                     /locus_tag="VW528_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEU9911656.1"
                     /translation="MSYLFLLCLTLFLLTIFYFFAFIQDLIAPPVVMSVMFLISSVFA
                     LVNSKNWNIEYSGIAYILIISGIIIFSLPLMALKSPNFNTEVKIADRLIDIQFWKIAL
                     TIIIDLFILYLYRKEIYNLVLSNGYTGSNIQWFFRNATSYEGELTVRTFIRVLIRVID
                     VSAYIFGYTFINNFLIYRHKRPKDILLLVPLLIFISKTLISGGRQDIIKILIAYVIMM
                     YIQQKRKVGWNRVISHKYIHLGFVGLIAGIPAFYYSLFLAGRSTTRTLFESVSTYLGG
                     SIQHFNQYIENPLDPGEVFGSETLVPILNILGEMGLVNYRSTIHLEFRTLGVTVGNVY
                     TFFRRPLHDFGLVGMYVFVFAVGAFFAIYYLVLRKKQVGFNLDIHTIIYSYVFYWIFL
                     SSIEQYSFTMISLYTLVFIVLVYFMAIFYWCTDFKRGKLIFKISDSSIKLKEE"
     gene            9079..10500
                     /locus_tag="VW528_000809"
     CDS             9079..10500
                     /locus_tag="VW528_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911657.1"
                     /translation="MNTKIKNIITSFSYVISSNLLIVLTSSLVVLIFPKIMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYTNLDRKQFYSQMILFSSFLMLISLVLF
                     TLNLITVRDENARYIYNMAIISMIVTNLRTLYVYILQMTNRLKDSSVILISDRVLYVL
                     LLFMFIVFGWHEYKVMIWADILGRTFSLMLSFWICKDIVFQPLSKFILDFKESLDNIR
                     VGINLMLSNIASSMIIGIVRMGIQWNWNIETFGKVSLTLSISNLLMTFINAIGLVIFP
                     LIKRTKTENLPKIYSNLRNALMLVMFAILLFYYPLKFILDIWLPAYKDALVFMALIFP
                     MSVYEGKMALVINTYLKAMRMEKDILKINALVMLTSMVVTLVTTLLLNNLGLTVVSIV
                     ILLALRSIIAELILSKKLKISVKQDIALELLMTIIFISSSWYLSIWIAVIIYLLAYTL
                     YLYLKHKDIRMYIEYFKNHKKIS"
     gene            10564..11652
                     /gene="wecB"
                     /locus_tag="VW528_000810"
     CDS             10564..11652
                     /gene="wecB"
                     /locus_tag="VW528_000810"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU9911658.1"
                     /translation="MKKIMLVFGTRPEAIKMCSLVNELRKQEDMKTVVCVTGQHKEMV
                     SPVLDLFGVQPDYDLEIMKANQNLFSITISILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIANYHFAPTELAKENLIKE
                     GRNNIYVTGNTVIDALTTTVQKDYTHPDLDLNDGNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYDDVKVIYPIHKNPLVRETATEIFGDTERIQIIEPLDVLDFHNFMNHSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKMLLDDPE
                     EYKKMSQASNPYGNGDASKQIVRILRGI"
     gene            11691..12560
                     /gene="rfbA"
                     /locus_tag="VW528_000811"
     CDS             11691..12560
                     /gene="rfbA"
                     /locus_tag="VW528_000811"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU9911659.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGLKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            12561..13157
                     /locus_tag="VW528_000812"
     CDS             12561..13157
                     /locus_tag="VW528_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU9911660.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            13167..14216
                     /gene="rfbB"
                     /locus_tag="VW528_000813"
     CDS             13167..14216
                     /gene="rfbB"
                     /locus_tag="VW528_000813"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU9911661.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            14282..15133
                     /gene="rfbD"
                     /locus_tag="VW528_000814"
     CDS             14282..15133
                     /gene="rfbD"
                     /locus_tag="VW528_000814"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU9911662.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITDAEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVARASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            15209..15643
                     /locus_tag="VW528_000815"
     CDS             15209..15643
                     /locus_tag="VW528_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9911663.1"
                     /translation="MLLVLVSLAHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTF
                     LSGKAENQVITLYHHDQCRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            15716..15973
                     /locus_tag="VW528_000816"
     CDS             15716..15973
                     /locus_tag="VW528_000816"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9911664.1"
                     /translation="MVNCEPLEAYRQLEEAELVGCWAHVRRKFFEVPPKQADKSSLGA
                     KGLAYCDQLFSLERDWEALPADERLQKRQEHLQPLMEDFFA"
     gene            16019..16165
                     /locus_tag="VW528_000817"
     CDS             16019..16165
                     /locus_tag="VW528_000817"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9911665.1"
                     /translation="MEYSLKYEETFKTILKDGHLVLSNNLAERAIKSLVMGRSKRVQW
                     TLLA"
     gene            16236..16394
                     /locus_tag="VW528_000818"
     CDS             16236..16394
                     /locus_tag="VW528_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU9911666.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            16593..18575
                     /locus_tag="VW528_000819"
     CDS             16593..18575
                     /locus_tag="VW528_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9911667.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDGYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVMAPVNEEFLNSKGDDFAKATDPNSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            18875..24193
                     /locus_tag="VW528_000820"
     CDS             18875..24193
                     /locus_tag="VW528_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9911668.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQ
                     NDNAGKPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYD
                     KDGWFWEYKTPGNSTWYKGNRVAAPETGSINRLSITLKSDGQLNASNNDVNLFDTVTL
                     PAAVNDHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKV
                     TYDTIQSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKIN
                     ETTAEYLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLL
                     SSISFLGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVS
                     TDKLAAGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFP
                     EYTKELPSAKVVIIEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFG
                     SQAQNPFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDF
                     KTLIEKAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDA
                     AYDLAHGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRF
                     AIEWGHGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAA
                     NYPLLGGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDN
                     GSTYKWTPEMRVELVDANNNKVVVTRKSNDVNSPQYRKRTVTLNGRVIQDGSAYLTPW
                     NWDANGKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVK
                     DGKITLDLLANQPYVFYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEI
                     VKSQGANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKE
                     VTTYTNKSLALNYVKAYAHNTRRDNATVDNTSYFQNMYAFFTTGSDVSNVTLTLSREA
                     GDQATYFDEIRTFENNSSMYGENHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRT
                     HLSEKHDPYTQRGWNGKKVDDVIDGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYR
                     VTFEYEAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTG
                     AETGDTWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALK
                     NYLPTVAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTA
                     LVADDFASLTAPAQAQEGLANAFDGNVSSLWHTSWGGGDVGKPATMVLKEPTEITGLR
                     YVPRGSGSNGNLRDVKLVVTDESGKEHTFTATDWPNNNKPKDIDFGKTIKAKKIVLTG
                     TKTYGDGGDKYQSAAELIFTRPQVAETPLDLSGYEAALVKAQKLTDKDNQEEVASVQA
                     SMKYATDNHLLTERMMEYFADYLNQLKDSATKPDAPTVEKPEFKLRSLASEQGKTPDY
                     KQEIARPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(24360..26519)
                     /locus_tag="VW528_000821"
     CDS             complement(24360..26519)
                     /locus_tag="VW528_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU9911669.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(26516..27112)
                     /gene="recU"
                     /locus_tag="VW528_000822"
     CDS             complement(26516..27112)
                     /gene="recU"
                     /locus_tag="VW528_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9911670.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            27178..27705
                     /locus_tag="VW528_000823"
     CDS             27178..27705
                     /locus_tag="VW528_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9911671.1"
                     /translation="MATALVLGYSTFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            27775..28104
                     /gene="gpsB"
                     /locus_tag="VW528_000824"
     CDS             27775..28104
                     /gene="gpsB"
                     /locus_tag="VW528_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9911672.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            28125..28505
                     /gene="rnpB"
                     /locus_tag="VW528_000825"
     ncRNA           28125..28505
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW528_000825"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            28590..29747
                     /locus_tag="VW528_000826"
     CDS             28590..29747
                     /locus_tag="VW528_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9911673.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARLPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFDEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            29760..31154
                     /gene="mapZ"
                     /locus_tag="VW528_000827"
     CDS             29760..31154
                     /gene="mapZ"
                     /locus_tag="VW528_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9911674.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            31230..32654
                     /gene="gndA"
                     /locus_tag="VW528_000828"
     CDS             31230..32654
                     /gene="gndA"
                     /locus_tag="VW528_000828"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9911675.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            32666..33355
                     /locus_tag="VW528_000829"
     CDS             32666..33355
                     /locus_tag="VW528_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9911676.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            33453..34415
                     /gene="cbpC"
                     /locus_tag="VW528_000830"
     CDS             33453..34415
                     /gene="cbpC"
                     /locus_tag="VW528_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9911677.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLRSSGAMA
                     TGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLDPSN
                     GDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            34434..35432
                     /gene="cbpJ"
                     /locus_tag="VW528_000831"
     CDS             34434..35432
                     /gene="cbpJ"
                     /locus_tag="VW528_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU9911678.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKELEIKTQDSVGRKYGEKRENSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYIGGERRAGWINDASAWYYLDPATGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(35550..36782)
                     /locus_tag="VW528_000832"
     CDS             complement(35550..36782)
                     /locus_tag="VW528_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9911679.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEIVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTLFLVLSPFSLI
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(36941..37411)
                     /locus_tag="VW528_000833"
     CDS             complement(36941..37411)
                     /locus_tag="VW528_000833"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911680.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCGIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKSNR"
     gene            complement(37311..37649)
                     /locus_tag="VW528_000834"
     CDS             complement(37311..37649)
                     /locus_tag="VW528_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
                     /protein_id="HEU9911681.1"
                     /translation="MTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANV
                     TAKKLIENYDTLEEIDFALGKSGVLLSLIKYYQFTNDNTLKTFIHNSIWGNLSLFPTK
                     RYSQRKHFRL"
BASE COUNT        12029 a         6408 c         8226 g        11399 t
ORIGIN      
        1 tttcctttct gttgataggc tagtagcaga cgattgattt gtctttcaga aagattgagt
       61 tcgacacagg cccgtttctt tgttttcttt ccttgggcta tagcttttat cacaagatat
      121 tttttcgttt cattcatatt cagttggatc cttttcatat gactattcta ccaaatggga
      181 cattttcacg ttcgatttac taaagacatt atcacattcg aattacatta aagatgcaga
      241 tagtaaaaaa aatgtagaca ttaccgtaaa aaagtgatat aatcgtatga tgttcaaggt
      301 ataggtgtta atcatgagta gacgttttaa aaaatcacgt tcacagaaag tgaagcgaag
      361 tgttaatatc gttttgctga ctatttattt attgttagtt tgttttttat tgttcttaat
      421 ctttaagtac aatatccttg cttttagata tcttaaccta gtgataactg cgttagtcct
      481 actagttgcc ttgctagggc tactcttgat tatctataaa aaagctgaaa agtttactat
      541 ttttctgttg gtgttctcta tccttgtcag ctctgtgtcg ctctttgcag tacagcagtt
      601 tgttggactg actaatcgtt taaatgcgac ttctaattac tcagaatatt caatcagtgt
      661 cgctgtttta gcagatagtg atatcgaaaa tgttacgcaa ctgacgagtg tgacagcacc
      721 gactgggact gataatgaaa atattcaaaa actactagct gatattaagt caagtcagaa
      781 taccgatttg acggtcaacc agagttcgtc ttacttggca gtttacaaga gtttgattgc
      841 aggggagact aaggccattg tcctaaatag tgtctttgaa aacatcatcg agtcagagta
      901 tccagactac gcatcgaaga taaaaaagat ttataccaag ggattcacta aaaaagtaga
      961 agctcctaag acgtctaaga atcagtcttt caatatctat gttagtggaa ttgacaccta
     1021 tggtcctatt agttcggtgt cgcgatcaga tgtcaatatc ctgatgactg tcaatcgaga
     1081 taccaagaaa atcctcttga ccacaacgcc acgtgatgcc tatgtaccaa tcgcagatgg
     1141 tggaaataat caaaaagata aattaaccca tgcaggcatt tatggagttg attcgtccat
     1201 tcacacctta gaaaatctct atggagtgga tatcaattac tatgtgcgat tgaacttcac
     1261 ttctttcttg aaaatgattg acttattggg aggggtagat gttcataatg atcaagagtt
     1321 ttcagctcta catgggaagt tccatttccc agtagggaat gtccacctag actctgagca
     1381 ggctctaggt tttgtacgtg aacgctactc actagccgat ggagaccgtg atcgtggtcg
     1441 caaccaacaa aaggtcattg tagcaattat tcagaagtta acttctacag aggttttgaa
     1501 aaactatagt agtattcttc aaggattgca ggattctctt caaacaaata tgccgattga
     1561 gactatgata gatttagtga atactcagtt ggaaagtggg gggaattata aagtaaattc
     1621 tcaagattta aaagggacag gtcggatgga tcttccttct tatgcaatgc cagacagtaa
     1681 cctctatgtg atggaaatag atgatagtag tttagctgta gttaaagcag ctatacagga
     1741 tgtgatggag ggtagatgaa atgatagaca tccattcgca tatcgttttt gatgtagatg
     1801 acggtcccaa gtcaagagag gaaagcaagg ctctcttggc agaatcctac aggcaggggg
     1861 tgcgaaccat tgtctctacc tctcaccgtc gcaagggcat gtttgaaact ccggaagaga
     1921 agatagcaga aaactttctt caggttcggg aaattgcaaa agaagtggca gatgatttag
     1981 tcattgctta tggcgcagag atatactata ctctggatgc tctagaaaag ctagaaaaaa
     2041 aagaaattcc tacccttaat gatagtcgtt atgccttgat tgagtttagc atgcatactt
     2101 cctatcgtca gattcatacg ggattgagca atattttgat gttgggaatc acgccagtaa
     2161 ttgctcatat tgaacgttat gatgctttag agaataacga aaaacgtgtt cgtgaactga
     2221 ttgatatggg gtgctatact cagataaata gttatcatgt ttcaaaacct aagttctttg
     2281 gtgaaaaata taaattcatg aaaaagagag ctcggtattt tttggaacgt gatttagttc
     2341 atgtagttgc aagtgacatg cacaatttag acagtagacc tccatatatg caacaggcat
     2401 atgatatcat tgctaagaaa tatggagcga aaaaagtaaa agaacttttt gtagataatc
     2461 ccagaaaaat tataatggat caattaattt aggagaaaat atgaaggaac aaaacacttt
     2521 ggaaatcgat gtattgcaac tattcagagc tttatggaaa agaaagttgg tcattttatt
     2581 agtggcaatt ataacttctt cagttgcttt tgcctacagt acttttgtta tcaaacctga
     2641 gtttactagt atgactcgga tttatgtagt taaccgtaat cagggagaga agtctggttt
     2701 aaccaatcaa gacttgcagg caggatcatc cttggttaaa gactatcgtg aaattatcct
     2761 atcgcaggat gttttggagg aagttgtttc tgatttgaaa ctagatttga cgccaaaaga
     2821 tttggctaat aaaattaaag taacagtacc agttgatacc cgtattgtct ctgtttcagt
     2881 tagtgatcga gttcctgaag aggcaagccg tatcgctaac tctttgagag aagtagctgc
     2941 tcaaaaaatt atcagtatta ctcgtgtttc tgatgtgaca acactggagg aggcaagacc
     3001 ggcgacatca ccgtcttcgc caaatattaa acgcagtaca ctaattggtt ttttggcagg
     3061 agtgattgga actagtgtta tagttcttat tcttgaactt ttggacactc gtgtgaaacg
     3121 tccgaaagat atcgaagata cactgcatat gacacttttg ggaattgtac caaacttgaa
     3181 taagttgaaa taggagagag gaatgccgac attagaaata gcacaaaaaa aactggagtt
     3241 cgttaagaag gcagaagaat attacaatgc cttgtgtaca aatatacagt tgagcggaga
     3301 taaactaaaa gtaatttccg ttacttctgt taaccctgag gaaggaaaaa caactacttc
     3361 cgtaaatata gcaaggtcgt ttgcgcgtgc aggctataaa actcttttga tcgatggcga
     3421 tactcgaaat tcagttatgt caggattttt taaatctcgt gaaaaaatta cagggctaac
     3481 ggaattttta tctgggacag ctgatttatc tcacggttta tgtgatacaa atattgaaaa
     3541 tttatttgta gttcaatcgg gcactgtatc accaaaccct acagccttgt tacaaagtaa
     3601 aaattttaat gatatgattg aaacattgcg taaatatttt gattatatca ttgttgatac
     3661 agcacctatt ggaattgtta ttgatgcggc aattatcact caaaagtgtg atgcgtccat
     3721 cttggtaaca gcaacaggtg aggtgaataa acgtgatgtc caaaaagcga aacaacaatt
     3781 agaacaaaca gggaaactgt tcctaggagt tgttttgaat aaattagata tctcggttga
     3841 taagtatgga gtttacggtt tctatggaaa ttatggtaaa aaaataattt aggaaagatt
     3901 ctatggatga aaaaggattg aaaatttttt tggcagtatt acagagtatt attgtcattt
     3961 tattggttta ttttcttagc tttgttagag agacagaact tgaacgttct tcgatggtta
     4021 tactatacct tctccacttt tttgtattct attttagttc ctatggtaac aattttttta
     4081 aaagagggta cctagttgag tttaatagta ccataagata tatttttttc tttgcaatag
     4141 ctataagtgt attaaacttt tttatagcgg aacggtttag tatctctaga agaggaatgg
     4201 tatacttctt aactttagaa ggaatatcct tatacttgtt aaatttctta gtaaagaaat
     4261 attggaagca tgtgtttttt aatctaaaaa atagcaagaa aattttactg ttaacagtaa
     4321 cgaaaaatat ggaaaaagtt cttgataaat tgctagaatc tgatgaactt tcatggaaat
     4381 tggtagcagt aagtgttttg gataaatctg attttcaaca tgataaaata cctgtaattg
     4441 aaaaggaaaa aattattgaa tttgcaacgc atgaagttgt ggatgaggtg tttgtcaatc
     4501 ttccaggaga gagctacgat attggagaaa ttatctctag gtttgagaca atggggatag
     4561 atgtaactgt aaatcttaaa gcatttgata agaatttggg tcgcaataaa caaattcatg
     4621 agatggtagg attgaatgta gtcactttct ctacaaattt ttataaaact agtcatgtaa
     4681 ttgctaagcg gattattgat atcatgggtg cattggtagg gctgatacta tgtggtttag
     4741 tcagtattgt attggttcct ttgattcgaa aggatggggg ctctgctatt tttgctcaga
     4801 cgcgtatagg aaaaaatggt cgtcagttca ctttttataa gtttcgctct atgtgtgtag
     4861 atgccgaggc gaaaaaaaga gaactcatgg aacaaaatac catgcagggt ggaatgttta
     4921 aggtggacga tgatcctcgt atcacgaaaa ttggtcgttt tatacggaag actagcttgg
     4981 acgagttacc acagttttat aatgttctaa agggagatat gagtttggtt ggcacacggc
     5041 cacctacagt ggacgagtat gttcagtata cttcagaaca gaaacgtcga ctcagcttta
     5101 aacctggtat tacaggttta tggcaggtta gcggccgtag taaaataacc gattttgacg
     5161 atgttgtaaa attagatgtg gcttatattg ataattggac aatctggaaa gatattgaaa
     5221 ttttgcttaa aactgttaaa gttgtattta tgagagatgg agcgaagtga ggaatgaggg
     5281 atagaatcca acttttaggt gtaacaattg atttgcttac gatgaatgaa acgatagata
     5341 ttgtagaaca atatgtatta gaaaaaagac cactacactt gatgggggtg aatgctgata
     5401 aaattaatca gtgtcataca gatgagaaaa tcaaaaaaat cgttaatgag tcaggaatca
     5461 ttaatgcgga tggagcatca gttgttcttg caagtaagtt tttaggaacg cctgttcctg
     5521 aacgagtagc gggtattgat ttgatgcaat gtcttttaga gttgtcaaat aaaaaaggat
     5581 attcagttta cttttttgga gcaaaagaag aagttttgca agatatgctc aaagtattta
     5641 agagagatta tccaaatttg atagttattg gacacagaaa tggctatttt tctgaagagg
     5701 atgaacaagc tattcaagaa gatattcgtg aaaagaaccc tgattttgtg tttattggaa
     5761 ttacgtctcc taaaaaagaa tatattattc aaaaatttat ggatagtggc gtcaattcgg
     5821 tatttatggg agttggcggt agttttgatg tcttgtctgg tcatatccaa cgagcacctc
     5881 tatggatgca aaagtcaaat ttagagtggt tattccgtgt agctaatgag cctaaacgtc
     5941 tctttaaacg ttattttgta gggaatattt cattcatagg aagagtttta aaagcaaaaa
     6001 gaggtgtaaa atattgaacc agacagagat gattcgctta attcaaaaag ttgaattaga
     6061 tgctataaaa gagtttaaaa aaatctgtga agagaatgat atagattttt tcctccgcgg
     6121 tggtagtgta cttggtgcgg tcaaatacga cggctttatt ccatgggatg atgatatgga
     6181 tatcgctgtc cctcgtgaag catacgacaa acttccaagt gttttcaaag atagaattat
     6241 cgctgggaaa tatcaggttc ttacttatca atactgtgat acgttgcatt gctactttcc
     6301 tcgactattc cttttagaag atgaaagaaa acgtttgggc ttgccacgaa ataccaatct
     6361 aggattgcat ttgattgata tcattccttt agatggagca ccaaatcatt cggttttaag
     6421 aaagatttac tttggcaaag tatactggta tcgtttttta gcaagtttag gaacaactta
     6481 tgttggcgac catgtggata tgcattccac taagcaaaaa ctaattattg gtttctttaa
     6541 aaaactagga tttgcaaaac tatttcctca aaattctgta tacagacgct tggataatct
     6601 ctataaaaag tatgattgga aaaagcagaa gtatgcgggg actatcaatg cttctttatt
     6661 tgctaaagaa gttatgccag tagagatttg gggagaagga gtagagaagc cttttgagga
     6721 tactttcttt aaagttccaa cggagtatga tcgctacctg aaaagacttt acggagaaaa
     6781 ctatctttac gaagagccta gtgatgatga aaagaaatcg catttaggag gacactaatt
     6841 tgttttgtta tattattttg cattacaaag tcttagaaga aactatttct tgtgttaaat
     6901 ctataaaaga aggcaattat aatgcaaagc aaatcgttat tattgataat ttttctaata
     6961 atggtactgg tgaaaaacta caagagcttt atgaatcaga tttagaaatt gatgttttga
     7021 ttaaccatga aaatgctggt tttgctcgtg gaaataatgt ggcttatcaa tttgctaagg
     7081 aaaagtataa ccccgatttc atggttatca tgaataacga tattgagata gaaacagaaa
     7141 attttgaaaa aattgtgaca gatatctatc gtgaggaaaa attccatttg ctcgggccag
     7201 atatcttctc gactacttac caacttcacc aaaacccaaa acggttgaca cattatactt
     7261 atggagaagt taaagctcta aatgaaaaat ttaaaaaagg gagccaagtt agtctagctt
     7321 taaaaatcaa atgttggttg aaagctagta aagttcttcg aacagcaatc tatcaaaata
     7381 gacgtaaaaa aggatcagta gactatagaa aacaggtaga aaacccaatt cttcatggtt
     7441 cttttattgt atattcgaga gattttatcg aaaaagagga gtatgctttt aaccctaaca
     7501 ccttcttcta ctatgaaaca gagatattag attatgaagc tgaattaaaa ggatacaaga
     7561 gaatttatac acctaaaatt agagttttgc accatcaaaa tgttgcaact aatcaagttt
     7621 acacgaactt gttagaaaaa accttgtttt caaataaatg caactttaaa tccaccagtt
     7681 attttttgaa gttgatgaaa gaaaacgagg atgtttaaat gagttattta tttttacttt
     7741 gccttacatt attcttattg actatattct atttctttgc ttttattcaa gatttaattg
     7801 ctcctccagt agttatgtct gtaatgtttc taattagttc agtatttgca ctggttaatt
     7861 caaaaaactg gaatattgaa tatagtggaa tagcctatat tctcataatt agtggtatta
     7921 ttatattttc gcttccttta atggcattaa aatcacctaa ttttaatact gaggttaaga
     7981 ttgctgatcg attaattgat atccaatttt ggaaaattgc tctaactatt ataattgatc
     8041 tctttatttt gtatctttac aggaaggaaa tatataacct tgttcttagt aatggatata
     8101 cggggtcaaa tattcagtgg ttttttagaa atgcaacgag ttatgaaggt gaattgacag
     8161 tgcgaacttt tattcgagtt ctcattcgtg ttattgacgt atctgcttat atttttggat
     8221 atacttttat taataatttt cttatctatc gccataaacg ccctaaagac atattacttt
     8281 tagtaccttt attaatattt atttcaaaaa ctttaatatc aggaggccgg caagatatta
     8341 ttaaaattct gattgcctat gtaatcatga tgtatatcca acaaaaacgg aaagttggat
     8401 ggaatagagt catatctcat aaatatattc accttggatt tgttggttta atagcaggta
     8461 ttccagcatt ttactactct ttgtttttag ccggtcgttc aacgactagg acgctatttg
     8521 agagtgtttc gacctatcta ggaggctcaa ttcagcattt taatcagtat attgaaaatc
     8581 cattagatcc tggtgaagtt tttggcagtg aaacattggt gcctatatta aatatattag
     8641 gggaaatggg cctagttaat tatcgtagta caattcattt agaatttcgg acactaggag
     8701 ttactgtagg aaatgtttat acttttttta gaagaccctt gcatgatttt ggtctagttg
     8761 gtatgtatgt gtttgtcttt gctgtaggtg ctttttttgc tatttattat ttagttctga
     8821 gaaagaaaca ggttggtttt aatttggata ttcataccat tatttattct tatgtctttt
     8881 attggatttt tttatcatca atcgagcaat actcgttcac aatgattagt ctatatacac
     8941 ttgtatttat tgtgttggtt tactttatgg ctatctttta ctggtgtaca gattttaaaa
     9001 gaggaaaact gatttttaaa atttctgact caagtatcaa attaaaagaa gaataacaga
     9061 atgtatagga gagggtagat gaatactaaa attaaaaata taataactag tttttcttat
     9121 gttatttctt caaatctgct catagtttta acctcatcac tagttgtttt gatttttcct
     9181 aaaataatgg gggtaactga gtacagttac tggcaacttt atatttttta tctgacctat
     9241 atcggttttt tccacttggg ttggattgat gggatttatc tcaaatatgg tggcttagaa
     9301 tatacaaatt tagatagaaa acagttttat tctcagatga ttctattttc tagtttctta
     9361 atgctaatct cgctggtatt atttactttg aacctaataa ctgtaaggga tgaaaacgca
     9421 agatatattt ataatatggc tatcattagc atgatagtca caaacttaag aacactctat
     9481 gtttatatct tgcagatgac aaatcgcttg aaggatagtt cagtcattct aattagtgat
     9541 cgcgttttat atgtactcct tttattcatg tttattgtat ttggatggca tgagtacaag
     9601 gtcatgattt gggctgatat tctaggtcga acattttctc tcatgctttc cttctggatt
     9661 tgtaaagata ttgtgtttca gcctttgtca aaatttatct tagatttcaa ggagtccctt
     9721 gataatatcc gtgttggaat caatttaatg ctatctaaca ttgcgagtag catgattata
     9781 ggcattgttc gtatgggaat tcaatggaat tggaatattg aaacattcgg gaaagtgtca
     9841 ttaactttga gtatatctaa tttattaatg acttttatta atgccattgg attagttatc
     9901 tttcctttga taaagcgaac aaagactgag aatttaccta aaatttattc taatttaaga
     9961 aatgctttga tgttggttat gttcgcaatc ttgctcttct actatccttt aaaatttatt
    10021 cttgatattt ggcttcctgc ttataaggat gccttagttt tcatggccct aatttttcct
    10081 atgtcagttt atgaagggaa aatggctttg gtgataaata catatttaaa agcaatgaga
    10141 atggaaaaag acattctcaa aattaatgct ttggttatgt taactagtat ggtagtgaca
    10201 ttagtgacta ctctactact aaataatttg gggctgacag ttgtatctat agttatttta
    10261 cttgctttaa gaagtataat agctgaatta attttatcca aaaaactgaa gatatcagtc
    10321 aagcaagaca ttgctttaga gttacttatg acgattatat ttatttcttc aagttggtat
    10381 ctctctattt ggattgcagt aataatttat ttattggcgt atactttata tttgtattta
    10441 aagcacaaag atatcagaat gtatatagaa tactttaaaa atcataaaaa aatatcataa
    10501 aaattatata tcaatgaaat ggtagattac atttctacct ttttatccat ttaggaggaa
    10561 acgatgaaaa agataatgct agttttcggt acacgtccag aagcaataaa aatgtgttca
    10621 ttagtcaatg agttgagaaa acaggaagat atgaaaacag ttgtttgtgt aactggtcaa
    10681 cacaaggaga tggttagccc tgttttagat ttatttggag ttcaaccaga ctatgattta
    10741 gaaattatga aggctaatca aaacttgttc tctataacaa taagtatttt agaaaaaata
    10801 aaacctgtgt tagaaaaaga acaaccagat attgttttgg ttcacggtga tactactacg
    10861 acatatgcag cagctttagc ggcattttat ctaggaatta aagttggtca tgttgaagct
    10921 ggattacgaa cttacaacct gcaaagtcct ttcccagaag agttcaacag acaatcgact
    10981 tctattattg caaattatca ttttgctcct acagaattag ctaaagaaaa tctaataaaa
    11041 gagggtagaa ataatatcta tgttactgga aatacagtga ttgatgcact tacaactaca
    11101 gtacaaaagg attatacaca ccccgatcta gatttaaacg atggaaatcg cctcatctta
    11161 ttgactgctc atagacgcga aaatctcgga gaacctatga gacatatgtt tagagctgtt
    11221 aaacgagttt taaatgaata tgacgatgtt aaagtaattt atccaattca taaaaatcca
    11281 ttggtacggg aaacagctac agaaattttt ggagatacag aacgtattca gatcattgaa
    11341 cctttagatg ttcttgattt tcacaacttt atgaatcata gttatatgat tctaactgac
    11401 tcaggagggg ttcaagaaga ggcaccttcg ttaggaaagc ctgtattggt catgcgagat
    11461 acgacagaaa gacctgaagg agtagctgct ggaacgttga aattggttgg gactgatgag
    11521 gagactattt atcaaaactt taagatgctt ttagacgatc ccgaagaata taaaaaaatg
    11581 agtcaagcta gtaatcctta tggaaatggt gatgctagta aacagattgt tcgaatttta
    11641 cgtggaattt gagtgtgttc agataaagta atatagaaag gtaccctact atgaaaggta
    11701 ttattctagc aggtggttcg gggacacgtt tatatccttt gactcgcgct gcatcaaaac
    11761 aacttatgcc ggtttatgat aaaccgatga tttactaccc actttcaaca ttgatgttgg
    11821 ctgggattag ggatattttg attatttcca ctccacagga tttacatcga ttccaagagc
    11881 ttcttcaaga cggatctgag tttgggctca aactttctta tgcagagcaa ccaagtccag
    11941 atggtttggc acaagccttt atcattgggg aagagtttat ttctgatgat agcgttgcgc
    12001 tgatcttagg tgacaatatc tatcacggtt ctgggctttc caagatgcta caaaaggcag
    12061 cgagtaagga gtcgggagca actgtttttg gctaccatgt caaggatcca gagcgctttg
    12121 gtgtggttga gtttgatcag gatatgaagg ctatttctat tgaagaaaag ccagagcaac
    12181 ctcgttcaaa ctatgcagtt acaggtctct atttctatga taatgatgta gtagagattg
    12241 ccaagagtat taaaccaagt cctcgtggtg aactggaaat tacagatgta aacaaggctt
    12301 acctagatcg tggtgatttg tctgttgagc ttatgggacg tggctttgct tggctggata
    12361 ctggaactca tgaaagttta ctagaggctt cacagtacat cgaaacagtg caacggatgc
    12421 aaaatgttca ggtagcaaac ttagaagaaa ttgcttaccg tatgggctat atcagtcgag
    12481 aagatgtatt ggccttagcc caaccactta agaaaaatga atacggacag tatctgctcc
    12541 gtttgattgg agaagcatag atgacagata attttttcgg taagacgctt gcggcacgca
    12601 aggttgaagc tattccaggc atgttggagt ttgatatccc cgttcatgga gataaccgtg
    12661 gctggtttaa agaaaatttc caaaaggaaa aaatgcttcc acttggattt ccagagtctt
    12721 tctttgcaga aggaaaattg caaaacaatg tatccttctc acgtaaaaat gtccttcgag
    12781 gtctccacgc agagccttgg gataagtaca tctctgtagc agatggaggg aaagttctgg
    12841 gttcttgggt tgatctacgc gagggtgaaa cctttgggaa tacctatcag acagtgattg
    12901 atgcgagtaa gggaatcttt gttcctcgag gcgtagctaa tggctttcaa gttttatcag
    12961 atacagtgtc atatagctat ctggtcaatg attactgggc tcttgaactc aaacccaagt
    13021 atgcctttgt gaactacgct gatccaagcc ttggtattga atgggaaaat attgcagaag
    13081 cagaggtttc agaagcagac aaacatcatc cactacttaa ggatgtaaaa cctttgaaaa
    13141 aagaagattt ggaataagga aagaatatga ctgaatacaa aaatattatc gtgacaggtg
    13201 gagctggctt tatcggttct aactttgtcc attatgttta cgagaacttt ccagatgttc
    13261 atgtgacagt cctagataag ttgacttatg ctggaaaccg cgcgaatatt gaggaaattt
    13321 taggtaatcg tgttgagtta gttgttggtg acattgctga tgcggagttg gtagacaagt
    13381 tggctgctca agcagatgct atcgttcatt atgcagcgga aagccacaat gataattcgc
    13441 tcaatgatcc atcgccattt attcatacta acttcatcgg aacctatact cttttagaag
    13501 ctgctcgtaa gtatgatatt cgcttccacc atgtatcgac agatgaagtt tatggggatc
    13561 tccctttacg cgaagatttg ccaggtcatg gtgaaggacc aggtgagaaa tttactgctg
    13621 aaacaaaata caacccaagc tctccgtact catcaaccaa ggcagcctca gatttgattg
    13681 tcaaagcctg ggtgcgttct tttggagtca aggcaacgat ttccaactgt tcaaataact
    13741 acggtcctta tcaacatatc gaaaaattca tcccacgtca gattactaac atcctaagtg
    13801 gtatcaagcc aaaactttac ggtgaaggta agaacgttcg tgattggatt cataccaatg
    13861 accattcttc aggagtttgg acaatcttga caaaagggca aatcggtgaa acctacttga
    13921 ttggggctga tggtgagaag aacaataagg aagttttgga acttatcctt aaggaaatgg
    13981 gacaagctgc ggatgcctat gatcatgtga ctgaccgtgc aggacatgac cttcgctatg
    14041 cgattgatgc tagcaagctc cgtgatgagt tggggtggaa acctgaattt accaactttg
    14101 aagctgggct caaggcaaca atcaagtggt atacagataa ccaagaatgg tggaaagcag
    14161 agaaagaagc tgttgaagcc aattatgcta agactcagga gattattaca gtataaaaag
    14221 caggaaatag ctgcttttta ttgctatatt gggaagagtt acatattaga aaggtctaga
    14281 gatgatttta attacagggg caaatggcca attaggaacg gaacttcgct atttattgga
    14341 tgaacgtaat gaagaatacg tggcagtaga tgtggctgag atggacatta ccgatgcaga
    14401 aatggttgag aaagtttttg aagaggtgaa accgacttta gtctaccact gtgcagccta
    14461 caccgctgtt gatgcagcag aggatgaagg aaaagagttg gacttcgcca tcaatgtgac
    14521 ggggacaaaa aatgtcgcaa gagcatctga aaagcatggt gcaactctag tttatatttc
    14581 tacggactat gtctttgacg gtaagaaacc agttggacaa gagtgggaag ttgatgaccg
    14641 accagatcca cagacagaat atggacgcac taagcgtatg ggggaagagt tagttgagaa
    14701 gcatgtgtct aatttctata ttatccgtac tgcctgggta tttggaaatt atggcaaaaa
    14761 cttcgttttt accatgcaaa atcttgcgaa aactcataag actttaacag ttgtaaatga
    14821 ccagtacggt cgtccgactt ggactcgtac cttggctgag ttcatgacct acctagctga
    14881 aaatcgtaag gaatttggtt attatcattt gtcaaatgat gcgacagaag acacaacatg
    14941 gtatgatttt gcagttgaaa ttttgaaaga tacagatgtc gaagtcaagc cagtagattc
    15001 cagtcaattt ccagccaaag ctaaacgtcc gctaaactca acgatgagcc tggccaaagc
    15061 caaagctact ggatttgtta ttccaacttg gcaagatgca ttgcaagaat tttacaaaca
    15121 agaagtgaga taagtagtag aatgattttc tagtctaata aaagaggcag ataatgaact
    15181 ccaaaggagc ttaagatgta cgattatctt gttgttggtg ctggtctctt tggcgcatag
    15241 ctttggctca gtttctatta tcgctcacac catccatcag aagtttaatc tgaaggtacc
    15301 caattatcgc caagaagaag attgggctag gatgggttta ccaatcacac gtaaggaaat
    15361 ctctaattgg catatcaagg caagtcaata ctatttagag tccctttata accttttacg
    15421 agaaaagttg ttagaacaac ctcttcttca tgcggatgaa acctcttatc gggtcttaga
    15481 aagtgatagt cagctgacct actattggac ctttttgtct gggaaagctg agaatcaagt
    15541 aatcacgctt taccaccatg atcagtgtcg gagtggttcg gtagtgcaag aattcctagg
    15601 agattattct ggctatgtgc attgtgatat gttgcggcag taacttagga ctttagtcct
    15661 ctagttctgt ctatgcgata gcagtccaag gtttaggagc aaggcgacgc taagcttggt
    15721 aaactgcgaa ccgctagaag cttatcgtca actggaagaa gctgaacttg ttggatgttg
    15781 ggcgcatgtg agaaggaaat tttttgaagt gccccccaag caagcagata aatcatcctt
    15841 aggagctaaa ggtttagctt attgtgatca gttattttcc ttggaaagag actgggaggc
    15901 tttgccagct gatgaacgac tacagaaacg tcaagaacat ctccaacccc taatggaaga
    15961 cttctttgct tagtgccgtc gtcagtcagt tttatcgggt tcaaaactag gaagggcaat
    16021 tgaatacagc ctcaagtatg aagaaacctt taagaccatt ttaaaagacg gacatctggt
    16081 cctttccaat aatctagctg aacgcgccat taaatcattg gttatgggac ggagtaaaag
    16141 agtccagtgg actcttttag cctaagctaa attttaaaaa gcgagggtgg ttattttctc
    16201 aaagttttga aggagctaaa gcaagagcta ttattatgag cttattggaa acagctaaac
    16261 gtcatcaact aaatagtgag aaatatctat cctatcttct agaatgtctt ccaaacgagg
    16321 aaactctcgt aaacaaagag gttttagagg cctatttacc atggactaaa gttgtacaag
    16381 aaaagtgcaa ataagaaatc tccagattag gaactatccg tgagttcact aatctggaga
    16441 tttttcaata gacctcgtta ttgggcggtt acgatattca tattttttgc aaagatgttg
    16501 tttgaaaaat aattttcaaa aattctgaaa attctgttga catctttctg aaaagagtct
    16561 ataatggaga gaaagtttta aaggagaaaa tgatgaaaag ttcaaaacta cttgcccttg
    16621 cgggcgtgac attattggcg gcgactactt tagctgcatg ctctggatca ggttcaagca
    16681 ctaaaggtga gaagacattc tcatacattt atgagacaga ccctgataac ctcaactatt
    16741 tgacaactgc taaggctgcg acagcaaata ttaccagtaa cgtggttgat ggtttgctag
    16801 aaaatgatcg ctacgggaac tttgtgccgt ctatggctga ggattggtct gtatccaagg
    16861 atggattgac ttacacttat actatccgta aggatgcaaa atggtatact tctgaaggtg
    16921 aagaatacgc ggcagtcaaa gctcaagact ttgtaacagg actaaaatat gctgctgata
    16981 aaaaatcaga tgctctttac cttgttcaag aatcaatcaa aggattggat ggctatgtaa
    17041 aaggggaaat caaagatttc tcacaagtag gaattaaggc tctggatgaa cagacagttc
    17101 agtacacttt gaacaaacca gaaagcttct ggaattctaa gacaacaatg ggagtaatgg
    17161 ctccagttaa tgaagagttt ttgaattcaa aaggggatga ttttgccaaa gctacggatc
    17221 caaatagtct cttgtataac ggtccttatt tgttgaaatc cattgtgacc aaatcttctg
    17281 ttgaatttgc gaaaaatccg aactactggg ataaggacaa tgtgcatatt gacaaagtta
    17341 aattgtcatt ctgggatggt caagatacca gcaaacctgc agaaaacttt aaagatggta
    17401 gccttacagc agctcgtctc tatccaacaa gtgcaagttt cgcagagctt gagaagagta
    17461 tgaaggacaa tattgtctat actcaacaag actctattac gtatttagtt ggtacaaata
    17521 ttgaccgtca gtcctataaa tacacatcta agaccagcga cgaacaaaag gcatcgacta
    17581 aaaaggctct cttaaacaag gatttccgtc aggctattgc ctttggtttt gatcgtacag
    17641 cctatgcctc tcagttgaat ggacaaactg gagcaagcaa aatcttacgt aatatctttg
    17701 ttccaccaac atttgttcaa gcagatggta aaaactttgg cgatatggtc aaagagaaat
    17761 tggttactta tggggatgaa tggaaggatg ttaatcttgc agattctcag gatggtcttt
    17821 acaatccaga aaaagccaag gctgaatttg ctaaagctaa atcagcctta caagcagaag
    17881 gtgtgacttt cccaattcat ttggatatgc cagttgacca gacagcaact acaaaagttc
    17941 agcgcgtcca atctatgaaa caatccttgg aagcaacttt aggagctgat aatgtcatta
    18001 ttgatatcca acaactacaa aaagacgaag taaacaatat tacatatttt gctgaaaatg
    18061 ctgctggcga agactgggat ttatcagata atgtcggttg gggtccagac tttgccgatc
    18121 catcaaccta ccttgatatc atcaaaccat ctgtaggaga aagtactaaa acatatttag
    18181 ggtttgactc aggggaagat aatgtagctg ctaaaaaagt aggtctatat gactacgaaa
    18241 aattggttac tgaggctggt gatgaggcta cagatgttgc taaacgctat gataaatacg
    18301 ctgcagccca agcttggttg acagatagtg ctttgattat tccaactaca tctcgtacag
    18361 ggcgtccaat cttgtctaag atggtaccat ttacaatacc atttgcattg tcaggaaata
    18421 aaggtacaag tgaaccaatc ttatataaat acttggaact tcaagacaag gcagtcactg
    18481 tagatgaata ccaaaaagct caggaaaaat ggatgaaaga aaaagaagag tctaataaaa
    18541 aggctcaaga agatctcgca aaacatgtga aataactgtt gcaaaatata agaaaggatt
    18601 tagtatttct cttgaatgct gaatcctttt ttacatttgt aaagaaagat tctaaatgta
    18661 ctgaccccca aaagttggag cctctttttg tcagaataga gaaaattttt gttaatttta
    18721 cttgtttcct attgctttct cagttattat ttgttatatt aaaagtataa ttatttttat
    18781 ttatcagggt taagcattgc attttcagag gaaggagtat tttttaaaaa ggaaatgtaa
    18841 acgcttgctc aaaaatgaaa ggatttagaa gtttatgaat aaaggattat ttgaaaaacg
    18901 ttgtaaatat agtattcgga aattttcatt aggtgttgct tctgttatga ttggagctgc
    18961 attctttggg acaagtccgg ttcttgcaga tagcgtgcag tctggttcca cggcgaactt
    19021 accagctgat ttagctactg ctcttgcaac agcaaaagag aatgatgggc gtgattttga
    19081 agcgcctaag gtgggagaag accaaggttc tccagaagtt acagatggac ctaagacaga
    19141 agaagaacta ttagcacttg aaaaagaaaa accggctgaa gaaaaaccaa aagaggataa
    19201 acctgcagct gctaaacctg aaacccctaa gacggtaacc cctaagacgg taactcctga
    19261 atggcaaacg gtagagaaaa aagaacaaca gggaacagtc actatccgag aagaaaaagg
    19321 tgtccgctac aaccaactat cctcaactgc tcaaaatgat aacgcaggca aaccagccct
    19381 gtttgaaaag aagggcttga ccgttgatgc caatggaaat gcaactgttg atttaacctt
    19441 caaagaagat tctgaaaagg gcaaatcacg ctttggtgtc ttcttgaaat ttaaagatac
    19501 caataataat gtttttgtcg gttatgacaa ggatggctgg ttctgggaat acaaaactcc
    19561 aggtaatagc acatggtata aaggcaaccg tgttgctgct cctgaaacag gatcaataaa
    19621 ccgtctttct atcactctca agtcagacgg tcagctaaat gccagcaata acgatgtcaa
    19681 tctctttgac acagtgactc taccagctgc ggtcaatgac catcttaaaa atgagaagaa
    19741 gattcttctc aaggcgggct cttatgacga tgagcgaaca gttgttagcg ttaaaacgga
    19801 taaccaagag ggggtaaaaa cagaggatac ccctgctgaa aaagaaacag gtcctgaagt
    19861 tgatgatagc aaggtgactt atgacacgat tcagtctaag gtcctcaaag cagtgattga
    19921 ccaagccttc cctcgtgtca aggaatacac cttgaatgga catactttgc caggacaggt
    19981 gcaacagttc aaccaagtct ttatcaataa ccaccgaatc acccctgaag tcacttataa
    20041 gaaaatcaat gagacaacag cagagtactt gatgaagctt cgcgatgatg ctcacttaat
    20101 caatgcggaa atgacagtac gcttgcaagt tgtggacaat caattgcact ttgatgtgac
    20161 caagattgtc aaccacaatc aagtcactcc aggtcaaaag atcgatgacg aaagaaaact
    20221 actttcttct attagtttcc tcggcaatgc tttagtctct gtttctagtg atcaaactgg
    20281 tgctaagttt gatggggcaa ccatgtcaaa caatacacat gtcagcggag atgatcatat
    20341 cgatgtaacc aatccaatga aggatttggc taagggttac atgtatggat ttgtttctac
    20401 agataagctt gctgctggtg tttggagtaa ctctcaaaac agctatggtg gtggttcgaa
    20461 tgactggact cgtttgacag cttataaaga aacagtcgga aatgccaact atgtaggaat
    20521 ccacagctct gaatggcaat gggaaaaagc ttataagggc attgttttcc cagaatacac
    20581 gaaggaactt ccaagtgcta aggttgttat cattgaagat gccaatgcag acaagaaagt
    20641 tgattggcag gatggtgcca ttgcttatcg tagcattatg aacaaccctc aaggttggga
    20701 aaaagttaag gatatcacag cttaccgtat cgcgatgaac tttggttctc aagcacaaaa
    20761 tccattcctt atgaccttgg atggtatcaa gaaaatcaat ctccacacag atggtcttgg
    20821 gcaaggtgtt ctccttaaag gatatggtag tgaaggtcat gactctggtc acttgaacta
    20881 tgctgatatt ggtaagcgta tcggtggtgt cgaagacttc aagaccctaa ttgagaaggc
    20941 taagaaatat ggagctcatc taggtatcca tgttaacgct tcagaaactt atcctgagtc
    21001 taaatatttc aatgaaaaaa ttctccgtaa gaatccagat ggaagctata gctatggttg
    21061 gaactggcta gatcaaggta tcaacattga tgctgcctat gacctagctc atggtcgttt
    21121 ggcacgttgg gaagatttga agaaaaaact tggtgacggt ctcgacttta tctatgtgga
    21181 cgtttggggt aatggtcaat caggtgataa cggtgcctgg gctacccacg ttcttgctaa
    21241 agaaattaac aaacaaggct ggcgctttgc gatcgagtgg ggccatggtg gtgagtacga
    21301 ctctaccttc catcactggg cagctgactt gacctacggt ggctacacca ataaaggtat
    21361 caacagtgcc atcacccgct ttatccgtaa ccaccaaaaa gatgcttggg taggggacta
    21421 cagaagttat ggtggtgcag ccaactatcc actgctaggt ggctacagca tgaaagactt
    21481 tgaaggctgg caaggaagaa gtgactacaa tggctatgta actaacttat ttgcccatga
    21541 cgtcatgacc aagtacttcc aacacttcac tgtaagtaaa tgggaaaatg gtacaccggt
    21601 gactatgacc gataacggta gcacctataa atggactcca gaaatgcgag tggaattggt
    21661 agatgctaac aataataaag tagttgtaac tcgtaagtca aatgatgtca atagtccaca
    21721 atatcgcaaa cgtacagtaa cgctcaacgg acgtgtcatc caagatggtt cagcttactt
    21781 gactccttgg aactgggatg caaatggtaa gaaactttct actgataagg aaaagatgta
    21841 ctacttcaat acgcaggccg gtgcaacaac ttggaccctt ccaagtgatt gggcaaagag
    21901 caaggtttac ctttacaagc taactgacca aggtaagaca gaagagcaag aactaactgt
    21961 aaaagatggt aaaattaccc tagatcttct agcaaatcaa ccatacgttt tctatcgttc
    22021 gaaacaaacc aatcctgaaa tgtcatggag tgaaggcatg cacatctatg atcaaggatt
    22081 taatagcggt accttgaaac actggaccat ttcaggcgat gcttctaagg cagaaattgt
    22141 caagtctcaa ggggcaaacg atatgcttcg tattcaagga aacaaagaaa aagttagtct
    22201 cactcagaaa ttaactggtt tgaaaccaaa taccaagtat gccgtttatg tcggtgtcga
    22261 taaccgtagt aatgccaagg cgagcatcac tgtaaacact ggtgaaaaag aagtgactac
    22321 ttataccaat aagtctctcg ctctcaacta tgttaaggcc tacgcccaca atacacgtcg
    22381 tgacaatgct acagttgaca atacaagtta cttccaaaac atgtacgcct tctttacaac
    22441 tggatcggac gtatcaaatg ttactcttac tttaagtcgc gaagctggtg atcaagcaac
    22501 ttactttgat gaaattcgta cctttgaaaa caattcaagc atgtacggag aaaaccatga
    22561 tacaggtaaa ggcaccttca aacaagactt tgaaaatgtt gctcagggta tcttcccatt
    22621 cgtagtgggt ggtgtcgaag gtgttgaaga caaccgcact cacttgtctg aaaagcatga
    22681 tccatataca caacgtggtt ggaacggtaa gaaagttgat gatgttatcg atggaaattg
    22741 gtcactcaag accaatggac tggttagccg tcgtaacttg gtttaccaaa ccatcccaca
    22801 aaacttccgc tttgaagctg gtaagaccta ccgtgtaacc tttgaatacg aagcaggttc
    22861 agataatacc tatgcttttg tagtcggtaa gggagaattc cagtcaggtc gtcgtggcac
    22921 tcaagcaagc aacttagaaa tgcatgaatt gccaaatact tggacagatt ccaagaaagc
    22981 caagaaggca accttcctcg tgacaggtgc agaaacagga gatacttggg taggtatcta
    23041 ctcaactgga aatgcaagta atactcgtgg tgattctggt ggaaatgcca acttccgtgg
    23101 ttataacgac ttcatgatgg ataatcttca aatcgaagaa attaccctaa caggtaagat
    23161 gttgacagaa aatgctctga agaactactt gccaacggtt gccatgacta actacaccaa
    23221 agagtctatg gatgctttga aagaggcggt ctttaacctc agtcaggccg atgatgatat
    23281 cagtgtggaa gaagcgcgtg cagagattgc caagattgaa gctttgaaga atgctttggt
    23341 tcagaagaaa acggctttgg tagcagatga ctttgcaagt cttacagctc ctgctcaggc
    23401 tcaagaaggt cttgcaaatg cctttgatgg caatgtgtct agtctatggc atacatcatg
    23461 gggcggagga gatgtaggca agcctgcaac catggtcttg aaagaaccaa ctgaaatcac
    23521 tggacttcgt tatgttccac gtggatcagg ttcaaatggt aacttgcgag atgtgaaact
    23581 tgttgtgaca gatgagtctg gcaaggagca tacctttact gcaactgatt ggccaaataa
    23641 caacaaacca aaagatattg actttggtaa gacaatcaag gctaagaaaa ttgtccttac
    23701 tggtaccaag acatacggag atggtggaga taaataccaa tctgcagcgg aacttatctt
    23761 tactcgtcca caggtagcag aaacacctct tgacttgtca ggctatgaag cagctttggt
    23821 taaggctcag aaattaacag acaaagacaa tcaagaggaa gtagctagcg ttcaggcaag
    23881 catgaaatat gcgacggata accatctctt gacggaaaga atgatggaat actttgcaga
    23941 ttatctcaac caattaaaag attctgctac gaaaccagat gctccaactg tagagaaacc
    24001 tgagtttaaa cttagatctt tagcttccga gcaaggtaag acgccagatt ataagcaaga
    24061 aatagctaga ccagaaacac ctgaacaaat cttgccagca acaggtgaga gtcaatctga
    24121 cacagccctc ttcctagcaa gtgttagcct agccctatct gctctctttg tagtaaaaac
    24181 gaagaaagac tagtatttag taaaacctct taacaagatt acggaagcag tctctatctt
    24241 ttccaatgag gtttatagta cagaaaaagc ctgagaagat atcttctcag gcttttgtta
    24301 agcacataaa tacaatagtg ctatgaaaaa atcacccaga aaaatctgga tgataaatgt
    24361 tatggttgtg ctggttgagg attctgattt tgttgatcag gggttgtatt tgattgttgc
    24421 gtattattgt taggattggt agtcgtacta ttatttgtgc ttggagtggt tgagctagac
    24481 tgtgaagttg aactatctga tgatgagctt gaactttcag ttgatggggg ttgttgtgga
    24541 gcaggtgagc tccacgtaga acgagcacca tttttaaata cgaattctcc atttctgtag
    24601 agcccctctg gtatattcca atcctctgga ttgcttcctt cagacaggta ggtcatcata
    24661 gagcggtaaa ctttggcagc gaccgtaagg ccattgccta caagtggtgt cagacggtta
    24721 gaatagcctg tccatacagc cattgaatat ttacgcgtat agccagcaaa tagttcatca
    24781 ggtgctacaa attgagaggt cttgatgtgg ttttcaattt cctcgtctgt atagttagag
    24841 gttcctgttt taccagcctg agggagccaa gcaagatagg catttcgtcc agttccataa
    24901 ctcaagactg ttttcatcat gtcggtcatc atataggctg tcgtttcctt catggcacga
    24961 gttccgacat tagagaactc tttttcactc ccatcactaa agacgacttt atggatatac
    25021 attggtttat agtaagttcc accatttgca aaggcagcgt aagcagcagc catcttttca
    25081 ctacttgctc catatttttt gtctgattcg gttgtgttac ttgaaatggc atttgagtag
    25141 tgaatacttg ggtagtcgat tccgagacca tttaggaaag tcttggcgcg gttgagtccg
    25201 accttgttta gagtttccac ggctgggacg tttcgcgatt gttgcagggc gtattgcaag
    25261 gtgatgttgc caaagtagcc cctatcccag ttataaacag gggtatttgt cccagggtag
    25321 ttatagggct catcgtgaac gatagtagca gttgaatcgt agacaccgta ctccaaggca
    25381 ggagcatagt ctgtgatcgg tttcatagtt gatccccagt cgcggtttgt ttctactgct
    25441 tggttaattc cgaaggaaac attacttgac tgatggcgtg ctcctagctg ggcaatgact
    25501 ttaccgttag aaacatcaac aatggtagaa gcgacttgca attcatcgtc tggataggca
    25561 acgtattcgt ctgtattgta aatatcccac agatgttttt gagcttcttg gtctacattt
    25621 gtgtagacat ccatcccagt tgtgagcagg ttatagcctg tttcttcttc cacttgattg
    25681 atgacttcct tgaggtaatt atccatgtaa gcagggtaat tacttgctga tttgagactt
    25741 tgtagtccat cagtaattgg tgtattgact gctttctcat actgttcagc agagatgtag
    25801 ccttgatttt tcatttcaga taagaccaag tttcggcggt cttgggctgc ttctggatgt
    25861 gaataggggt catattggtt tggtgcctga ggcattccag ccagcaaggc taactgaggt
    25921 aaacttaaat tattgaggtc tttaccatag tagttttgag ctgctgtctg cattccatag
    25981 ttcccattag acatgtagac cttatttata tagtaggtca agatttcttg cttggttgct
    26041 ttttgttcta actgaatcgc taaccaagct tcctgagcct tacgagaaat agtctggtcg
    26101 gaagtcgaag ttgaaaagta agtcaactta atcaactgtt gggtgagagt tgatccacct
    26161 tggagggaat tgctttgcag attgcgcaag aaagctccca ggatacggat ggtatcaatc
    26221 cccctgtggt cgaagaagcg atggtcttcg atagaaacga ttgccttaac caaatctgtg
    26281 ggaatatcat tagcttgggc attgacgcgg cgttcagaac ccaagtcagc aatgagttga
    26341 tttttattgt cgtagatttt actagaagtt gttgcaacta gtttactctc ggataggcta
    26401 ggagccttgc taacgtagta gaaaaaaact cctccgccta agacaatggc tgcgataacc
    26461 aagcttaaga agctaatgct cagatacttg attaggcgca gaatcgttgg tttgttcatc
    26521 ttgttttacc acctaataaa tgttctttga taacattgag ataaggaatt tgagggaagg
    26581 caccagcctt gatttcatat ccatattctc gaatatattc aagtggcatt gatttttgtc
    26641 ccttatcttg atgatagaag cgaatcaaat cgaatgccgg caataagtag gtttcttgct
    26701 gagaagaaaa gtgaagaagg acaaagcaga ttccttgttg ggcaaggact tgttccatat
    26761 gctgaatctg atgtggatga aaatttttca tcggaatcgc acgtttttgt tttgtttcct
    26821 tgacttcaaa gtcgatgtaa tatccattat aaacgccaga atagtccgtc gttgaagctt
    26881 gtcgaaaata ggcttcaaca atcttggcac gacttcgttg tggatagtcc acttgtacga
    26941 tttgaatagg agttggtttc ttatgtataa cagccaagcc ctgagacaaa tagtagtcgt
    27001 tggtagcatt gatcatcttt tcaaaagaca ttcctcgatt tgcgaaattt ttgggttgag
    27061 aaagagatgt ttgtcttttt tgtgatgaaa ctttatgtgg atagttgacc ataattctcc
    27121 ttattggtac aataacatca ctttattata ccataatttg cacagaaagg gttaaaaatg
    27181 gctacagctt tggttttagg ctattctact tttgatttgg gtttattttc tgataaggat
    27241 cctcgtctta aattgattaa aaaagcgata cgtaaagatt tagaggctat ggcagcagat
    27301 ggggtgtctt ggcttgtgtt tacaggaagt ttgggttttg aatattgggt tttagaggtt
    27361 gctcaggaga tgaaaaccga atacggtttc cagttggcca ccatttttgc ttttgaaacc
    27421 catggggaaa attggaatga aggtaatcag atgaaactga gtcgctttaa gcaggtagac
    27481 tttgtcaaat atgcttatcc tcgctatgaa cacaaggggc agttaagaga ttaccagcag
    27541 tttttgctgg aaaacacgac tagttcctat cttttttatg atgaagaaaa tgaaacaaaa
    27601 ctagcttatt tttaccaaaa gatgaaaaat caagaggact attttataaa gagattaaca
    27661 tttgatcaat taaatgaact tgctgaaaat ttttccgaaa attgaagctt tgaccttgat
    27721 ttttgtttgc ctttttttat ataataatac tagcaagcga gaatggagag agacatggca
    27781 agtattattt tttcagcgaa agatattttt gaacaagagt ttggacgtga agtccgtggc
    27841 tataataaag tagaagttga cgagttttta gacgatgtca tcaaggacta tgaaacctat
    27901 gctgccttgg tcaagtcact tcgtcaggaa attgcggatt tgaaggaaga attaactcgt
    27961 aaaccgaaac cttcaccagt tcaagcagaa ccccttgaag cggcaattac aagttctatg
    28021 acgaattttg atattttgaa acgcctgaat agattggaaa aagaagtttt tggtaaacaa
    28081 attttagata actcagattt ttaagtagtt atttgagatg tgcaattttt ggataatcgc
    28141 gtgaggagaa ttgcttctca tgaggaaagt ccatgctagc acaggctgtg atgcctgtag
    28201 tgtttgtgct aggcgaaacc ataagcctag ggacgagaaa tcgttacggc agttgaaatg
    28261 gctaagtcct tggataggcc agagtaggct tgaaagtgcc acagtgacgg agtctttctg
    28321 gaaacagaga gagtggaacg cggtaaaccc ctcaagctag caacccaaat tttggtcggg
    28381 gcatggagta tgcggaaacg aacgtagtat tctgactgct atcagctaga gctgttagtg
    28441 gtagacagat gattatcgaa ggaagtggtc ctagtcactt ctggaacaaa acatggctta
    28501 tagaaaattg catataggtt ggggctgaga aattttctca acctcatttt ttaaagtgga
    28561 catatagaaa ggtcttgcaa gactgtaaca tgaaaaaaga atttaattta attgcaactg
    28621 tggcagcagg gcttgaggct gtcgttggtc gtgaagtgcg agagttgggc tacgattgtc
    28681 aggttgaaaa tggacgtgtt cgttttcaag gagacgtgag agctattatc gaaaccaacc
    28741 tttggcttcg ggcagcagat cgtatcaaaa ttatcgtagg aacgttccca gctaagactt
    28801 ttgaagagct atttcaggga gttttcgctt tagattggga aaattattta ccacttggag
    28861 ctcggttacc gatttcaaaa gctaaatgtg ttaagtccaa acttcacaat gagcccagtg
    28921 ttcaggctat ttctaagaaa gctgttgtca agaaattgca gaaacactat gctcgcccag
    28981 aaggggttcc tctgatggag aatggcccag agtttaagat tgaggtctct attctcaaag
    29041 atgtggcaac tgtcatgatt gatacgaccg ggtctagcct ctttaaacgt ggttatcgta
    29101 ccgaaaaagg tggcgctcct atcaaggaaa atatggcagc agccatttta caactttcta
    29161 actggtatcc agacaagcct ttgattgatc cgacctgtgg ttcggggact ttctgtattg
    29221 aggcagttat gattgctaga aagatggcgc caggtcttcg tcgctctttt gcatttgatg
    29281 aatggaactg gatcagcgat cgcttgattc aagaagtgcg cacagaagcg gctaaaaaag
    29341 tagaacgtaa gcttgagttg gatatcatgg gctgtgatat tgatgctcgc atggtggaaa
    29401 ttgctaaggc caatgctcag gcagctggtg ttgcaggaga tattactttt aagcagatgc
    29461 gcgtgcagga tttacgttcc gataaaatca atggagtaat catttccaat ccgccttatg
    29521 gtgaacgttt gtcagatgat gcaggagtga ccaagctcta tgctgagatg gggcaagtat
    29581 ttgcaccgct gaaaacttgg agcaaattta tcctgactag tgatgaagct tttgaaagca
    29641 agtatggtag ccaagcagat aagaagcgta agttatacaa cggaaccttg aaagtggatc
    29701 tatatcaata ttttggtcag cgtgtcaaac ggcaagaggt aaaatagaaa gggatactca
    29761 tgagtaaaaa aagacgaaat cgtcataaaa aagaaggtca agaaccgcaa tttgattttg
    29821 atgaagcaaa agagctaaca gttggtcaag ctattcgtaa aaatgaagaa gtggaagcag
    29881 gagtcttgcc tgaggattcc attttggaca agtatgttaa gcaacacaga gatgaaattg
    29941 aggcggataa gtttgcgact cgtcaataca aaaaagagga gttcgttgaa actcagagtc
    30001 tggatgattt aattcaagag atgcgtgagg ctgtagagaa gtcagaagct tcttcggagg
    30061 aagttccatc ttctgaagac atcttactac ccttgcctct ggacgatgag gagcaaggct
    30121 tggatcctct attgctagat gatgaaaatc caacagaaat gactgaagaa gtggaagagg
    30181 agcaaaacct ttctcgtctg gatcaagagg actcagaaaa gaaaagtaaa aaaggcttta
    30241 ttttgaccgt tttggcgctt gtatcagtaa ttatttgtgt cagtgcttat tatgtctatc
    30301 gtcaagtggc tcgttcgacc aaggaaattg aaacttctca atcaactaca gccaatcaat
    30361 cggatgtgga tgattttaat acactttatg acgcctttta cacaaatagc aataaaacgg
    30421 ctttgaaaaa tagccagttt gataaactga gtcaactcaa gactttactt gataagctgg
    30481 aaggtagtcg tgaacatacg cttgccaaat ctaaatatga tagtctagta acgcaaatca
    30541 aggccattca agatgtcaat gcacaatttg aaaaaccagc tattgtggat ggtgtgttgg
    30601 ataccaatgc caaagccaaa tcggatgcta aatttacgga tattaaaact ggaaatacgg
    30661 agcttgataa agtgctagat aaggctatca gtcttggtaa gagccagcaa acaagtactt
    30721 ctagctcaag ttcaagtcaa actagcagct caagttcaag tcaagcaagt tcaaatacga
    30781 ctagtgagcc aaaaccaagt agttcaaatg agactagaag tagtcgcagt gaagtcaata
    30841 tgggtctctc gagtgcaggg gttgctgttc aaagaagtgc cagtcgtgtt gcctataatc
    30901 agtctgctat tgatgatagt aataactctg cctgggattt tgcggatggt gttttggaac
    30961 aaattctagc aacttcacgt tcacgtggct atatcactgg agaccaatat atccttgaac
    31021 gtgtcaatat cgttaacggc aatggttatt acaacctcta taagccagat ggaacctatc
    31081 tctttaccct taactgtaag acaggctact ttgtcggaaa tggcgctggt catgcggatg
    31141 acttggacta ctaagcagtc gttacaaaat tctttctttt caaaagtaaa aatgataaaa
    31201 taaaacaaat gaaacaagag gagtgtcaaa tgacaaaagc taactttggt gtcgtaggta
    31261 tggccgtaat gggtcgtaac cttgccctta atattgaatc acgtggttac acagttgcta
    31321 tctacaaccg tagtaaagaa aaaacggaag atgtgattgc ttgccatcct gaaaagaatt
    31381 ttgtaccaag ctatgacgtt gaaagttttg taaactcaat cgaaaaacct cgtcgtatca
    31441 tgctgatggt tcaagctgga cctggtacag atgctactat ccaagccctt cttccacacc
    31501 ttgacaaagg tgatatcttg attgacggag gaaacacttt ctacaaagat accatccgtc
    31561 gtaatgaaga attggcaaac tcaggtatca actttatcgg tactggagtt tctggtggtg
    31621 aaaaaggtgc ccttgaaggt ccttctatca tgcctggtgg acaaaaagag gcctacgaat
    31681 tggtttcgga tgttcttgaa gaaatctcag ctaaagcacc agaagatggc aaaccatgtg
    31741 tgacttatat cggtcctgat ggagctggtc actatgtgaa aatggttcac aacggtatcg
    31801 agtacggtga tatgcaattg atcgcagaaa gctatgactt gatgcaacac ttgctaggcc
    31861 tttctgcaga ggatatggct gaaatcttta ctgagtggaa caagggtgaa ttggatagct
    31921 acttgatcga aatcacagct gatatcttga gccgtaaaga cgatgaagac caagatggac
    31981 caatcgtaga ctacatcctt gatgctgcag gtaacaaggg aactggtaaa tggacgagcc
    32041 aatcatctct tgaccttggt gtaccattgt cactgattac tgagtcagtg tttgcacgct
    32101 acatttcaac ttacaaagaa gaacgtgtac atgctagcaa ggtgcttcca aaaccagctg
    32161 ccttcaactt tgaaggagac aaggctgaat tgattgaaaa aatccgtcaa gccctttact
    32221 tctcaaaaat catttcatac gcacaaggat ttgctcaatt gcgtgtagcc tctaaagaaa
    32281 acaactggaa cttgccattt gcagatatcg catctatctg gcgtgatggc tgtatcatcc
    32341 gttctcgttt cttgcaaaag attacagatg cttacaaccg cgatgcagat cttgccaacc
    32401 ttcttttgga cgagtacttc ttggatgtta ctgctaagta tcaacaagca gtgcgtgata
    32461 tcgtagctct tgcggttcaa gctggtgtgc cagtgccaac tttctcagca gctattactt
    32521 actttgatag ctaccgttca gctgaccttc cggctaactt gatccaagca caacgtgact
    32581 actttggtgc tcacacttac caacgtaaag acaaagaagg aaccttccac tactcttggt
    32641 atgacgaaaa ataagtaggt cagccatggg gaaacggatt ttattacttg agaaagaacg
    32701 aaatctagct cattttttaa gtttggaact ccagaaagag cagtatcggg ttgatctggt
    32761 agaggagggg caaaaagccc tttccatggc tcttcagaca gactatgatt tgattttatt
    32821 gaacgttaat ctgggagata tgatggctca ggattttgca gaaaaattga gtcgaactaa
    32881 acctgcctca gtcatcatga ttttagatca ttgggaagac ttgcaagaag agctggaagt
    32941 tgttcagcgt tttgcagttt catacatcta taagccagtc tttatcgaaa atctggtagc
    33001 gcgtatttcg gcgatcttcc gaggtcggga cttcattgat caacactgca gtctgatgaa
    33061 agttccaagg acctaccgca atcttaggat agatgttgaa catcacacgg tttatcgtgg
    33121 tgaagagatg attgctctga cccgccgtga gtatgacctt ttggcgacac ttatgggaag
    33181 caagaaagta ttgactcgtg agcaattgtt ggaaagtgtt tggaagtatg aaagtgcgac
    33241 cgagacaaat atcgtagatg tctatatccg ctatctacgg agcaagcttg atgttaaagg
    33301 acaaaaaagc tacattaaaa ccgtgcgtgg tgttggttac accatgcaag aatagaaaag
    33361 cagttgcagt tgtgtaactg cttttttgag gagtttttat atattgacat gcagtcgtgt
    33421 ctttgctaca atcgttttag gaggatatat aaatgaaact tttgaaaaaa atgatgcaag
    33481 ttctactagc agtctttttc tttggtttgc tagctacaaa tacggtattt gcgaatacca
    33541 caggtggccg atttgttgat aaggataata gaaaatatta tgtaaaagat gatcataaag
    33601 caatctattg gcataaaata gacggtaaaa cttactattt tggtgatatt ggagagatgg
    33661 ttgtcggttg gcaatactta gaaattcctg gaacaggtta tcgtgatgat ttatttgata
    33721 atcgtccagt cttcgaaatt ggccttcagg agaagtggta ctattttgga caagatggtg
    33781 ctttgctaga acaaacagat aaacaagtac tagaggcaaa aacgtctgaa aatacaggaa
    33841 aagtatacgg tgaacaatat cctctatctg ctgaaaagag aacttattat tttgataata
    33901 attatgctgt aaagacaggc tggatttatg aagacggcaa ttggtattat ttaaataagc
    33961 taggaaattt tggtgatgat tcttacaatc cactaccaat tggtgaagtt gctaagggtt
    34021 ggactcaaga ttttcatgtt actattgaca ttgatagaag caaacctgct ccatggtact
    34081 acctccgttc ctcaggagca atggccactg gctggtatca ggaaggtacc acttggtatt
    34141 atttagacca gccaaatggc gatatgaaaa caggttggca aaaccttggg aacaaatggt
    34201 actatctccg ttcctcagga gctatggcaa ctggttggta tcaagatggt tcaacttggt
    34261 actatttaga tccttctaat ggagatatga aaataggttg gacaaaagta aatggaaaat
    34321 ggtattatct caattcaaat ggagcaatgg ttacaggtag tcaaactatc gatggtaaag
    34381 tttataactt cgcttcatct ggtgagtgga tttaatgttg gaggatatat aaaatgaagc
    34441 ttttgaaaaa aactatgcaa gctggactaa cagtaatttt ctttggtttg ctagctacga
    34501 atacagtatt tgcagataac tctgaaggat ggcagtttgt ccaagaaaac ggtagaacct
    34561 attacaaaaa gggggacctc aaagaaacct actggcgagt gattgatggt aagtactatt
    34621 attttgattc tctatctgga gagatggttg tcggctggca atatatcccg tttccatcta
    34681 aaggtagtac aattggtcct tacccaaatg gtatcagatt agaaggtttt ccaaagtcag
    34741 agtggtacta ctttgataaa aatggagtgc tacaagagtt tgttggttgg aaagaattag
    34801 agattaaaac tcaagacagt gttggaagaa agtatgggga aaaacgtgaa aattcagaag
    34861 ataaagaaga gaagcgttat tacacgaact attactttaa tcaaaatcat tctttagaga
    34921 caggttggct ttatgatcag tctaactggt attatctagc taaaacagaa attaatggag
    34981 aaaactatat tggtggtgaa agacgtgcag gttggataaa tgatgcttca gcttggtact
    35041 acctagatcc agcaactggt attatgcaaa caggttggca atatctaggt aataagtggt
    35101 actacctccg ttcctcagga gcaatggcca ctggctggta tcaggaaggt accacttggt
    35161 attatttaga ccacccaaat ggcgatatga aaacaggttg gcaaaacctt gggaacaaat
    35221 ggtactatct ccgttcatca ggagctatgg caactggttg gtatcaagat ggttcaactt
    35281 ggtactacct aaatgcaggt aatggagaca tgaagacagg ttggttccag gtcaatggca
    35341 actggtacta tgcttatagc tcaggtgctt tggcagtgaa tacgaccgta gatggctatt
    35401 ctgtcaacta taatggcgaa tgggttcggt aatgacaggg cgcttataat tatattagaa
    35461 cactcgctta atcaaagaag tgactttgtt gtacttgacc tgacgattgg ggagattaaa
    35521 agcatgtgaa atctacacat gctttttcgt tagttatgag tgttgaactt gactaaccat
    35581 tgacaaaaga taagagcaat caaaataaga tacaaaaata tcaatgctaa ctgcaccgaa
    35641 acgcccgatg ctatggatag ggctaatagg gagagcaggg acggggcgag aatgtctact
    35701 gtgctcagag cagagcccac cgtccccatc ttgtcactgg ggatatgggc aaagacagct
    35761 gcttgtaggc gagggctgag aatgccgata atcgtagaac tgatgaaact ggtcacgaaa
    35821 attacgatat aggcttgaca aaggatacta gttattagag ccaataggga aatctcacag
    35881 aaaaccaaga ggtgcgtgaa acggatattt ttaaatagat tactgctggt catattgcca
    35941 aggatagctc cacaagacat gatgatggag agcagggcga agagttgccc aatgctcagt
    36001 tgtgctagtg tctgttggat gaagcggagg gcaatcagcc gaggggacac atccaagatg
    36061 gcaatcatac tggtcgtcag aaacagtagt atgacggtat cttttaacct caggagcaca
    36121 gccagcgact gtttaagatg ttgaaaatat ttcttaaaac tcagtgctgt atgtgacatt
    36181 tcaatccttt tttcaacctc atacaaggta tgtcgaataa catacaggcc caaaaaggca
    36241 atgacaaaag tcagagtgtt gataagggaa atagtttgaa tactcataac attgataagg
    36301 aatgcgccag ctagattggc gacaatgcgg acaaccctca tcagagactg cctgaacccc
    36361 atggcatcat gcaggtcgtc cttaattaca ctgatgtaga gggcgttcat catgtaggca
    36421 gacaggtagc tcaaggtgtc agagatgagg ttgatgatga caatcataat gactgaaatt
    36481 aatgaaaagg gcgacaagac gagaaatagt gtcaaaatag cgtagagcag gattttgatt
    36541 ttggcaatac agagtgcgtg tttgactcta ttttcttgaa aatctcctag aatcccgaga
    36601 aagagttgcg atagtagggg aactatctct gaaatggaaa tcagtaaaat cgctagcgga
    36661 gcgaaattgt actgagccac ataattgata atggcgaggt agaaaatact gtcgccgaat
    36721 gatgaaaaac agctgtttag cagcaactgt ctatatatgt tgtttgtcca aaataatttc
    36781 atacttttct ccttatgtct gcacatcgaa tgggaattta ttatttaata gacaccaata
    36841 taccccctgc ttcctattcc gaagtcaaac aaatctgctt taccactatc tccttgaaac
    36901 atcagtaaac cgtgatcatc tcgttcagaa catgctaaaa ttacctgttg gattttttca
    36961 tcagtaattt gttttgattg tagacagtgg tttgtagtaa gctagttatt ccgtggcaat
    37021 atcctgtcat catttttaga tgatggttaa aaacaaattc ttgatattta ctaataatat
    37081 ctttgtttcc gtcacaatcg tacatacaaa gatataagat tattccggaa attccttcgc
    37141 accaagaaag ttgtaaattt cctaaatttt cagtattaga agtaactttt tctaataatt
    37201 tttttaattc agcatggaat gtatggagat cattatagaa catagaaggt tctaagactt
    37261 tagaataggc aaataaagca tatgcaattc cacaatatcc atgagcaaag ctatagtcta
    37321 aaatgctttc tttggctgta tctctttgta ggaaataatg ataaatttcc ccatatacta
    37381 ttgtggatga aagttttaag agtattgtca ttggtaaatt gatagtattt tattaatgat
    37441 aataggacac cagattttcc caatgcaaag tctatttcct ctagagtatc ataattctct
    37501 attaatttct tagcggtgac gtttgctaat tgtagatagt attgattttt actgatagaa
    37561 aataaatgta ggagtaacca tagatagcct gcatcaccaa atagcagtga gtattgatat
    37621 tcatatgtat gtgtcgttct aattgtcact gccttcttga tgatagagag tattttagaa
    37681 tccagttctg gggggtacaa tttatttaga ggaaacagta gtccagctat tccgtgttgg
    37741 aatgataaat tacttacgaa atttctaaat tctgttgaag aagtgatcct tccttcagaa
    37801 tttacagttt tatctagtaa aaattgtaga acactattac attctgtttt gtaatcatat
    37861 ttatgttcct ctattttttt aattttgact aaattatttg tttgacttaa atttagtaga
    37921 cattgtaacc aaaatagttg ctcatctgtg agctgacctt gagtatgtga tagttctagt
    37981 aattgaatag gatcctttag gtagattcaa tcaatctata acacaacaac ataataacac
    38041 ttttaggata acttatcaaa ta
//
LOCUS       DAVKQB010000017        36213 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKQB010000017 DAVKQB010000000
VERSION     DAVKQB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36213
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.017"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..516)
                     /locus_tag="VW528_000835"
                     /pseudo
     CDS             complement(1..516)
                     /locus_tag="VW528_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            1004..1123
                     /locus_tag="VW528_000836"
     CDS             1004..1123
                     /locus_tag="VW528_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9911682.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            complement(1219..2565)
                     /gene="glnA"
                     /locus_tag="VW528_000837"
     CDS             complement(1219..2565)
                     /gene="glnA"
                     /locus_tag="VW528_000837"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9911683.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(2602..2958)
                     /gene="glnR"
                     /locus_tag="VW528_000838"
     CDS             complement(2602..2958)
                     /gene="glnR"
                     /locus_tag="VW528_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9911684.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            complement(3035..3562)
                     /locus_tag="VW528_000839"
     CDS             complement(3035..3562)
                     /locus_tag="VW528_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9911685.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRVRLFLEKKEK"
     gene            complement(3698..4894)
                     /locus_tag="VW528_000840"
     CDS             complement(3698..4894)
                     /locus_tag="VW528_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9911686.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(4984..9963)
                     /locus_tag="VW528_000841"
     CDS             complement(4984..9963)
                     /locus_tag="VW528_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU9911687.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLHYQGHEVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELTFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNKVLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            10256..11887
                     /locus_tag="VW528_000842"
     CDS             10256..11887
                     /locus_tag="VW528_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU9911688.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGIGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNIIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            complement(12428..14035)
                     /locus_tag="VW528_000843"
     CDS             complement(12428..14035)
                     /locus_tag="VW528_000843"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9911689.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENRN"
     gene            complement(14425..15012)
                     /locus_tag="VW528_000844"
     CDS             complement(14425..15012)
                     /locus_tag="VW528_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9911690.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(15140..15589)
                     /locus_tag="VW528_000845"
     CDS             complement(15140..15589)
                     /locus_tag="VW528_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9911691.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(15594..15983)
                     /locus_tag="VW528_000846"
     CDS             complement(15594..15983)
                     /locus_tag="VW528_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU9911692.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(16011..16637)
                     /locus_tag="VW528_000847"
     CDS             complement(16011..16637)
                     /locus_tag="VW528_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9911693.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVFASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(16651..17214)
                     /locus_tag="VW528_000848"
     CDS             complement(16651..17214)
                     /locus_tag="VW528_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9911694.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(17379..17528)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(17588..18091)
                     /locus_tag="VW528_000849"
     CDS             complement(17588..18091)
                     /locus_tag="VW528_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9911695.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(18284..19114)
                     /locus_tag="VW528_000850"
     CDS             complement(18284..19114)
                     /locus_tag="VW528_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9911696.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRLLV
                     LIAIFSVFLLYLSEIHFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGAYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(19099..20781)
                     /locus_tag="VW528_000851"
     CDS             complement(19099..20781)
                     /locus_tag="VW528_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911697.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHCPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLRTELVKETPELQSLFRLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKALSSFIQTEGRYLWEGQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEQEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQQGHTIVMITHDMQLMLDYSDRALVMVDGELIA
                     DTDPASLLSNPELLVKANLKETSIFNLAKKLDVDPLALTAFYKERREGCKLN"
     gene            complement(20883..21140)
                     /locus_tag="VW528_000852"
     CDS             complement(20883..21140)
                     /locus_tag="VW528_000852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911698.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(21147..21695)
                     /locus_tag="VW528_000853"
     CDS             complement(21147..21695)
                     /locus_tag="VW528_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9911699.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(21710..22558)
                     /locus_tag="VW528_000854"
     CDS             complement(21710..22558)
                     /locus_tag="VW528_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9911700.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            22746..24095
                     /gene="trkA"
                     /locus_tag="VW528_000855"
     CDS             22746..24095
                     /gene="trkA"
                     /locus_tag="VW528_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9911701.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            24099..25538
                     /locus_tag="VW528_000856"
     CDS             24099..25538
                     /locus_tag="VW528_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9911702.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMAFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMTIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLSLIFIVSLDSNDFLIVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(25663..27342)
                     /locus_tag="VW528_000857"
     CDS             complement(25663..27342)
                     /locus_tag="VW528_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9911703.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSVMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
     gene            complement(27411..28823)
                     /gene="lacG"
                     /locus_tag="VW528_000858"
     CDS             complement(27411..28823)
                     /gene="lacG"
                     /locus_tag="VW528_000858"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9911704.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVQYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(28833..29177)
                     /locus_tag="VW528_000859"
     CDS             complement(28833..29177)
                     /locus_tag="VW528_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9911705.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(29345..31231)
                     /locus_tag="VW528_000860"
     CDS             complement(29345..31231)
                     /locus_tag="VW528_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU9911706.1"
                     /translation="MVIRVFDQQKNTYSSFALEELRYYMNQVFNTDIELVEEKEADIF
                     VGLVNEEDGKDHIFISLNKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFIP
                     ELRFEDFLDKELSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYKDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(31233..32555)
                     /locus_tag="VW528_000861"
     CDS             complement(31233..32555)
                     /locus_tag="VW528_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9911707.1"
                     /translation="MNNILTFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRIFIQRGITIKMPNGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            32723..33946
                     /locus_tag="VW528_000862"
     CDS             32723..33946
                     /locus_tag="VW528_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9911708.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPSVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTATKYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKKFDFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            34957..35241
                     /locus_tag="VW528_000863"
     CDS             34957..35241
                     /locus_tag="VW528_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911709.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDIEKL
                     LDSKELTHFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            35234..35791
                     /locus_tag="VW528_000864"
     CDS             35234..35791
                     /locus_tag="VW528_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter peripheral membrane
                     component"
                     /protein_id="HEU9911710.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNTHEELWLNNHVANMNKQPPLSLCESLYSFENLTVL
                     VVPIEYVLGMKMMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVLLEG
                     FSYAYGMDWLEKFFKENQDKLREFY"
BASE COUNT        11060 a         7773 c         6280 g        11100 t
ORIGIN      
        1 tattcgttgt tgttcggata gtggggggag agcaattaat aatagattaa aattataatc
       61 attgattgca ggataacttg ttccagtaga tttattatta acacgattga taaaattatc
      121 tgataataaa taatatttca aatatgtttc gttaagtaaa gtatccaaaa caataaatgc
      181 tgtactagct atcaaatact ctttaagttc tctaactaca gcaatatttt ttagatatgg
      241 tctaactgtt gaaaataaga cactattctg cgaaactaat tttctagcac gggaaggcgc
      301 ttgttcaggt gaaagatatt gtagattttt gtagttgatt atgttctttt ttctatcaat
      361 actagacgta tctatatacc taaaggattt ctctggctta ttttgcccaa aattccaata
      421 aattgatttt atcctcaccc actcccaagt atcaggaata tcataaggaa catcaatttc
      481 ttgagtactt ccatcagcaa acttcccata atgtttctta tgtgcttcaa gtatataaaa
      541 aggcgtaaaa atacgcctat agataatggg gttgaaatag gtttattgtt gatgagattg
      601 tagataattc aattttttac ttccaatcga atattcaaaa cctccacctt ttctgcctgt
      661 aattgttcat cataaaattc aatatcttca ggattttccc cttggcaacc tcggcagaaa
      721 tattcttccg ctcgatcagg attcaaaaat cgacaagcac aaacaaaaca gtcgccatca
      781 tcatttattg agataatata gtagattgaa ataagatgta aacaaatcga ttaggaaagt
      841 taaattagtt tctagaaatt tttagcagat gtagtgtacc attctagtct caatttacta
      901 tggcttcaaa tatatctttc gaaaaaatat ttacagatgt gtaattttga agcttgcaaa
      961 agttagtaaa cttgtagatt tcgatttgaa gtaacttgtt ttcttgcccg atattgtttt
     1021 tgaaattgaa tttttccata gtgactcctt aattttcttc tacacgtctg atgataaatc
     1081 taattcgcaa aagagtcaag aggatttttc gaaaaataaa tagcgaccga aatcgctatt
     1141 ttaagggtta taggtatttg atggcttaga ctgctgtgtg actgtttacc cacaggcaat
     1201 ctttcttcta tattagtatt agtaaaggtc taaataatta tcaatttccc attgtgaaac
     1261 gaaggttgca taacttgccc attcgattcg tttggcttca aggaagctag tgtagatgtg
     1321 atctccgaga gcagctttaa ccacttcatc ttctgtcaaa gctttcaaag cgttgtgaag
     1381 agttgatgga aggtctgtaa taccagcttc cttgcgctct tctgctgtca tgatgtagat
     1441 attttcttcg ataggagctg gtgcttcgat tttattttca ataccataca aaccaacttc
     1501 caaaagaaca gccatagcaa tgtaagggtt cgccattgga tccactgaac gcaactcaag
     1561 acgagttccc ataccacgtg aagcaggtac gcgcacaagt ggcgaacggt tacgaccagc
     1621 ccaagcaatg taaacaggcg cttcataacc tggaaccaaa cgtttgtatg agttaactgt
     1681 tgggttcatg atggcagtat agttgtaagc atgcttgatc aaaccgccta ggaaatggta
     1741 agctgtttct gacaactgca ttccttttgg atcatttgga tcaaagaagg cgttatttcc
     1801 ttctgcatca aacaaggaca tattacagtg catacctgag ccagcaatac caaattttgg
     1861 cttcgccata aatgttgcgt aaagtccgtg tttgcgagca atggttttaa caacaagttt
     1921 aaagatttga atcttatcac aagcacggag aacttcatcg tacttaaagt caatctcatg
     1981 ctgtccaacc gcaacctcgt ggtgactcgc ttctacttca aatcccattt tggtcaagac
     2041 attcacaatc tcacgacgtg tgttgtccgc aaggtcagta ggtgccaaat caaagtagcc
     2101 acccttgtca ttcacttcaa gtgttgggtc cccattttca tccaacttaa ataggaagaa
     2161 ttctggctct ggaccaaggt tgaaggattt gaatccaact tcttccatgt gacgaagagc
     2221 tcgtttcaaa ttaccacgag ggtcacccgc aaatggttca ccttctgttg tatagacatc
     2281 acagatcaga cctgcaacac ttccattttc atctccccaa gggaagactg tccatgtatc
     2341 caagtccggg tacaagtaca tatccgactc attgatacgt acaaaacctt caatagaaga
     2401 tccatcaaac ataaccttgt tcgacaaaac cttatctaac tgttcatctg tagcaggaat
     2461 ttcgacgttt ttcatggttc ccaaaatatc tgagaacata agacgaataa aggtaacatt
     2521 tttttccttg acttcacgac gaatatctgc agctgtgatt ggcataagtt ttctccttaa
     2581 tctatgacta cttgcggttg cctaaccgcg accaaaaggt gactgtactg aagcaaaacg
     2641 cccctgttgg aggagttcat tgtgaagtgc acgacgtact tcagtctgac taaccgcttt
     2701 cttggatttc gcttcacgtt cagcatattt tttcttaatg gcagcgatat tataaccttc
     2761 agagatataa tctttgattt caagcagacg atccatgtca ttcaaggaat acatgcgacg
     2821 atttccttcg tttcgatcgg gcttgatcaa ctcttgatct tcataataac gaatctgacg
     2881 cgccgataga tcggtcaact tcataacact gccgatagga aaaacagcca tatttcggcg
     2941 aaattctttt tccttcattt acaatttcct tctttctgtc tattatagtc taaaaaaaga
     3001 caaacgtcaa ttgataatgt tataaaatgt aacattattt ttcttttttc tctaaaaaga
     3061 gacgaacacg atcaatatcg taatttacga taattgcgac aaaaactccc ataaacgttt
     3121 ctgatacacg cacaaacacg tacgaaattg tctcaccact tggaattgat agggtaatga
     3181 ttaacatagc tgctacacca ccaataaccc ctgctttgtt attcatggct acatttgtca
     3241 taatggttaa catggtgcag attggaacaa ctaccaaggt cacccaaaag gcttcgtgga
     3301 aaaaggtatt taataagaag aagaccaagg catagagtcc accgatacta tttcctagaa
     3361 tacgcgaagt cccaaaatga acactctcat caaaactctc cctcaggcta aaaacggctg
     3421 tcaaagcacc aatttgaaga cctttccagc caaaaaagcc aaaaatcaag agaactagaa
     3481 aaacagcaat acctgtttta aaggttcgca taccaagttt gaactgggat ttatcgaatt
     3541 tatatttttt aaaataactc ataatctcaa ctttctattt ccattttatc ataaatcggt
     3601 gatttttatg agtaatagtt gagaggaagc gtttttattt taagcaaaag aaaagaggaa
     3661 ctttcatccc tctcttcttt gatttgttta taaaatctta tttttctgtc agggctgcaa
     3721 gtcctggaag aaccttacct tcaagaagtt ccattgatgc tccaccaccc gtactaatcc
     3781 atgagaactt gtctgcacgg ccaaggttaa tcgctgcggc agctgagtca ccaccaccga
     3841 tgattgattt aactcctggt tgtttcacga tagcgtccat cacaccgatt gtaccagctt
     3901 ggaaatctgg gttttcaaat acacccatag gtccgttcca tacaactgtt ttggcaccag
     3961 tcaaagcttc gtcaaatttg gcgatagatt ttggaccgat gtcaagacca aggaagcctt
     4021 cagaaactgc ttcaccttca gtgtcacgca cttcagtgta accagcaaat gcgttagctt
     4081 cttttgagtc aactggcaag atcaatttac catttgcttt ttcaagaaga gctttcgcaa
     4141 catccaattt gtcttcttct acaagtgagt taccgatttc gataccttgt gctttgtaga
     4201 atgtgtaagt catcccacca ccgataagga ctttatcagc tttttcaagc aagttttcga
     4261 taacaccgat cttgtctgaa acttttgaac caccaaggat agccacgaat ggacgttctg
     4321 gagtttcaac tgcttcttgg atgtaggcaa tttcgttttc aagaaggaaa ccagcaactg
     4381 ctttttcaac gtttgctgag ataccaacgt tagatgcgtg tgcacggtga gctgtaccga
     4441 atgcatcgtt tacgaagata ccatctccaa gtgatgccca gtatttacca agttcaggat
     4501 cgtttttaga ttctttcttg ccgtcaacat cttcgtaacg agtgttttca accaagagaa
     4561 cttgtccatc ttcaagagcg ttgattgccg cttccaattc agcaccacga gtgacacctg
     4621 ggaaaacaac atcttgacca agttttgctg ccaagtcagc tgctacagga gcaagtgatt
     4681 taccagcttt atcagcttct tctttcacac gtccaaggtg agagaaaaga attgcacgtc
     4741 caccttgttc gatgatgtac ttaatagttg gaagagctgc tgtgatacgg ttatcgttag
     4801 tgattacgcc atctttcaat ggtacgttga agtcaacacg aacgaggact tttttacctt
     4861 tcaagtcaac gtctttaaca gtaagttttg ccatgttaca aaaactcctt tatttatttt
     4921 attcgaaact attatatcac actttggaaa tataaggaaa gatttcacaa gatttttcga
     4981 tatttaatct ttatcttctc ttttccgtct caaggtcaag gctaaactag caagaccgag
     5041 gctggctaag cctgctatta gggcttcatt agcatcagct gttcctgtat ttggcaattg
     5101 tttgcttgct tcttctgatt taactgctgt attttgagct ggtttttcct gtgtagctac
     5161 tactgctggt actgttttta cttttgttcc aatttcaaca atttctggaa tcgcttcttg
     5221 gatgacttct gttgaacgaa gacgacgctg accgttttca tcaacttcaa agacatgtct
     5281 taacagtcca tctcgtccct gtatgatgac tcgttgttcc ccaactagca tctcagcatt
     5341 ttcatgctct tgacgatgaa aggcaacctt ttcctcttga acttccaatt taggagcctt
     5401 ttgttgatca gaagtcggtt gataagtagg ttttggaagg actttgtttt gtggtgctgg
     5461 tttagcaggt tgtggttttt cagcagattc ataaacaaag gcataatgag taaagtgtgg
     5521 tgctgtaaag ataacatgac tatccgtttg ttcaaaagtc aattctactg cctctttgcc
     5581 ttcaggtaag aaaaatactt tcttaacttt cttatctttt tcaattggga ttttcacaat
     5641 agaagcgtaa gagagatcaa catcttgacc tttttcatcc aaaccttcta tttcaaagac
     5701 atgagcatct tctccagcaa agtatttctt ctcttcagca cttgcttgaa cacgctctac
     5761 tttcaaaccc ttgatgacag tcttctcttt atttgagaag tgtacttcaa cacctgtttt
     5821 actatcagaa aagactgctg gcttgtcttc tttcaataaa gcaaccagat cttttagact
     5881 ctgtttcgct gttgtaagct cttctggact caaatctact ttttcaacca aggccttgtt
     5941 tttctcaaga agcggagcta gggcagaacc tgaaggatgg tttggtttgg cagccaagag
     6001 ttcttgaacc tcacctgtta agcgatcaag ctctgtcttt tcttccgtaa atttctcttg
     6061 accattcaga gctttatgag cttctgtcaa tttattaagt cggtcattga cttgatcttg
     6121 ggtgctagtt tcatggtctt tcaacacttg ttcggccgct tcaattgctt tcaagaaggc
     6181 ttcttttact tctgatgaag caaagttata gaccaccttg ttcttagcag cttcaacttc
     6241 tgctaatttt tgtttgaggt actcatcgtt caatgctgct tttggagata ccaaaacatc
     6301 aaagctaact tcatggcctt ggtaatgaag cgtcaaggtt tgacgaccag tcttttgagc
     6361 atcgtaacca gaaatttcaa ctccctcatc agtaaaggaa tgttcttcca tggtgtcatt
     6421 gctataagcc actgcaaagc gtccttcaga caagtctaag ctatcaccaa ctaggtaatc
     6481 ttttttcggt tcctgactta cttcaattcc caaaatagtt ttcggacttg cttcgtcttg
     6541 gccagtgaca gtcactgaca aattagcatt taccggttgt cccaaatatt ggagagtaag
     6601 attctgttct cccttatgat gcgtatcaaa acctgatact gatacacctg cgtgagttag
     6661 gcgaatgagt tcgtcctcag ttcctccttc atactgaact cgaagaacac cacctctaag
     6721 gtccaaatcc tccccttcgg cgtagcttgt tttcttaggt cctgtttcta ggctgactga
     6781 cttgattctt ctgtcatctt ttggaacgga aactgctagg acattactaa tttccttgcc
     6841 tggcaactgg gtacgataat aaagaagacc agattgattt gtcaaatcca atggtgcact
     6901 tgctaggtct cctccaactt cgctcttcaa ggttgcgagc ggagtttgag aatttggatt
     6961 atcataaacg gtaatagttg ctccagccgg tacatctgca aagccaactt gtaccttgtt
     7021 attgcctaga gaacgggctg cagccttggc catcggaata ttgacactct cagtatcaag
     7081 cttttcatac attttccagt tatagatacg aatagccttc catggagttc cattgtcaga
     7141 agtgacaaca ttcaagcgcc agtcttgagc agtgattggt ttatcaagag tgatatctgt
     7201 cacgtgtgct ttgttaccac ggacttcctt agctagcttc cactcaccat ctgcatcttt
     7261 ataataaagg tcaaagtctt tagtgttcat caagccatcg ttaacagact caccaccagc
     7321 tcctgcatga tccatgaccc atctaacaac ggtacgtggc ttggtcaaac gaatatccac
     7381 actaccactc aactgagctg aagaccattt atctgacaag ctagtaatgg taccgttcaa
     7441 cataccttca ataccttctc caccttcagt cttagggaaa gtactatcaa taactgttgc
     7501 acctggaacg atattttcag ctagtggttt tggtaggctg gtatctttta cagtcatacc
     7561 ccaatcaaag gttgtggttg cagcttctga acgaactcca tttttaccga ctgctacaac
     7621 cttcagttct tgagttgtac cctgagcact tgctgagcgg ctaacttttg gtagataaat
     7681 agttgtagaa gatgagccag ttagtaattt ccagctgtct ccatcttttt cataaacttc
     7741 atagaaatct gcatccttgt tgcctttaaa ttgcacaact gcttccgctt cttgggcatt
     7801 tttaagagat tgtttcacta cagaaaagct tgtcggcgat tgtggctctt ggtgattgtc
     7861 cgagatagtt aattgtccta ggttaaactg ataatctttt acagcacctt catgctcgaa
     7921 aaatagtttg actgcataga tggttttacc cgctagtgag ctaagatcaa attcttcatt
     7981 tgtccagttg tcagaaaggg ttagctcttt ccatgcatct gcatcatcga atttgtagtc
     8041 tggagttgta gagaatgcca tataaacttt agaacctttt cctcccttgt gggcaacacg
     8101 aagtttggtc ttctcagtta cttctaactt agtagaataa agtctcacat cctgatctgt
     8161 cttaccagct acatcaccag agaatttaag ggaatttccg ccattatagg catctgtaaa
     8221 atcatattct gcacgaagtt tttcccctgt tgaagtctgc caccagcgcc atgttggaag
     8281 aacacctgat actgaacgat aattccactc agaatcctta gaaaccttac catctacgaa
     8341 ccatttttta ccatgacctg tattaaaaga agtagtaaaa gtattaccta ccgctggcgt
     8401 acggtccgca actaggttag caataccata ccaatctttg tcacctggtt tttggccagt
     8461 ggggtctcct tgataacctg taaagaagat atcttcattt ttatgataat cttcaccagt
     8521 ttttcctaaa cttgtaatgg tatctggggc aaataaacca agagaaaggc gcaatttccc
     8581 attttcgtct aaaatgtcat tccacttaac ctttgtcttg taggaaccac cctgttgcaa
     8641 ttccaaacct gcaaatacat cataaggatt acgaccaatc cagttggcag ttgcaatagt
     8701 gtaatcattt ttagccttat cccagttaaa gttagcaaag aagttatctg ccggaacctt
     8761 atctccttct ggttgcatga attggtagtt gtattctccc aaaccatctt gatggtaacg
     8821 tccatagtta taggtcatgg catcatacca agaatacttg attggatggt ttaccttagc
     8881 agcatattcc ttgctataga gcataaactg gcgcatcttt tctccaagag gtttaaccaa
     8941 atctccagtt gtttcttggt tgatgaaata gccatcatag ccataatact ttgccatgtc
     9001 taccaattta cgggcaattg ggaagctacc atctgcgtct tgcttcaaag cttcagcaaa
     9061 tctttcttga tctgcaatac tattagacca gttgaagaag agtgtaccgt atacaggaac
     9121 cccgttacgg tgacctgcat caataacgtc aggagttggt acgagacctt cccagaagac
     9181 cattgaatct agatattgcc aatagtcaaa agcataggcc ttgaactctt ctccaccaac
     9241 agaagcatgg tcttttgctt tagaattggt gtttgataag gcttgaactt ttgcttcctt
     9301 gctagctttt tcattgacta aatgacctgt ccgacgtgaa gcgaggacaa cagatccgcg
     9361 gttaatagca tcgtcttcac gagcacctgg ttcccatttt aacaattctt cccaagaatt
     9421 aaatttgatt tcctttggtt ttaatgtttt ttctgtattt ttagggacat ctggctggac
     9481 ttctttttca gtcttattag cttctgctgc tggtttagac tcatcccctt ggttagttgc
     9541 ttcgggcttt gcttctgttt ccttgtcaga cttaggtttt gcttctgcag atggtgtttc
     9601 tgcaaccacc tcacttgctt tttcctctgg tttagctgcg ctagctgctt ctgcagtttc
     9661 agtagttgca gcttcattgc ttgctggcga agcagtttca gatgctatcg ctactccttc
     9721 tgccttttct agcttgtttt ctctagcatg ttccccttca ttctgcttat cagcttcatt
     9781 ggtcgaatgc tcactctctc ctgaaacaag ctctgtatta gctccgctat tttcaggaac
     9841 tgcagtttct tcagccaagg ctggaccagc aaataaaaca gcaccaatca tcagcgagca
     9901 ggctcctact gataacttac gaatactgta acgacaacgt ctttcaaaaa atggattctt
     9961 cattttctcc tcctaaaaga ttgattttgt tgttttcaag aaagcgcttt atattctgta
    10021 gttttttatc aaactctaga accatgataa tcacattttt atcctaaaac atttttagac
    10081 aaaaaacaat agctgctttc ataaatctaa aataaggtta aaaactctta aaaataatac
    10141 acttatcaat tataattttc ttttacaaac ttgacgttaa cacttgttag atttactttt
    10201 atcctttaaa atagaatagg agatattccg cgattatttt tcggaggaaa aagaaatgtc
    10261 cattaattgg caggaaattt tatttcactt tttaggtggt ctggggctat tcttatatag
    10321 catcaagacc atgggagacg gtttgcaaca agctgctgga gatcgccttc gtttttacat
    10381 tgacaaatat actagtaatc ctttctttgg agttctggtt ggtattggga tgactgctct
    10441 aattcagtct agttctggtg taacagttat tacagtcggc ctggtcagtg ccggtctctt
    10501 aaccttacgt caggctatcg ggattgtcat gggtgctaat attgggacaa ctgtcacatc
    10561 ctttctcatc ggttttaaat taggtaacta tgccctacct atgctcttta tcggtgccgt
    10621 ctgtctcttt tttacgaaaa atcggacagt caataatatc ggacgcatcc tctttggtat
    10681 cggtggtatc ttttttgccc tcaatctcat gagcggcgca atggctccac tcaaggattt
    10741 acaggtcttt aaggactata tgattgagct aagtaagaat cctgttttgg gtgtctttgt
    10801 cggtactggc ttgaccttgc taattcaagc ttcttcggct accattggga ttttacaaaa
    10861 cctctacgcc ggcaatctaa ttgacctaca gggagctttg ccagttctat ttggtgacaa
    10921 tatcgggaca accattacag ccatcattgc ctctttaggg gctaatattg cagctaaacg
    10981 ggtagcagga gctcatgttg ccttcaacat tatcggaaca gtcgtctgcg ttatttttct
    11041 agttcctttt actgtcctga ttcattggtt tgaagctacg ctaaatctag caccggaaat
    11101 gaccatcgcc tttgctcacg gaacctttaa tattaccaac accattgtcc aatttccatt
    11161 tatcggagct ctggcttact ttgtaaccaa gattattcct ggagaggacg aggttgtcaa
    11221 atacgaaccc ttatatcttg atgaacattt catcaaacag gccccatcta tcgctctagg
    11281 aaatgctaag aaagagctct tgcacttagg aaactacgct gctaaagcct ttgacctttc
    11341 ctataagtac atcattgact tggatgaaaa agttgctgaa aaagggcata aaaccgaaga
    11401 agcaattaac accatcgatg agcaattaac acgttatctc attgcccttt caagcgaagc
    11461 tctcagccaa aaagaaagtg aagtgcttac caatatcctt gattcctccc gtgatttgga
    11521 acggattgga gaccacacgg aggctctact caatctgact gactatcttc aacggaaaaa
    11581 tgttgaattt tctgatgccg ccttgaaaga attagaggaa gtttaccgcc aaactagtga
    11641 ctttatcaaa gatgctctgg atagtgtgga aaacaatgat attgaaaaag cacgcagtct
    11701 tgtagaacgt catgaagcaa tcaataagat agaacgtgtt ctcagaaaaa cccacatcaa
    11761 acgcctcaac aaaggcgaat gttcaacaca agctggggtc aactttatcg acatcatctc
    11821 acactacact cgtgtatcag accacgctat gaaccttgct gaaaaggttt ttgcagaaca
    11881 aatctaataa ccaagaagct atccatcata attggatggc ttcttacttt ttcctaagca
    11941 agactaggct gaatgaaact gaaagagtat tctgcagata tatagtatat tgaaactaga
    12001 atagtacacc tctatttcta aaacattgtt agaaatcgat ttgtcctgtt attatttcat
    12061 tttactataa tggattaaat taaaaaagta tagtgtattg aatctataac agtccatctt
    12121 gactgctaaa atatttctat aaattaattt gacttttctg atagagatgt tcacatctta
    12181 tttcaattca tgatacaatc cagttcctaa aactttgcta gaaattaatt tggctttccg
    12241 aattaatttg ttcagatttt atttcatttc aatatactga taaatccatt cactagcaca
    12301 gggcaagagg gagtcgctat tgatttcaac ggtagcaggt acatacagag gactcctacc
    12361 tcagatatac tgcaatatca tcgtgtgcaa aaaacctctg agatttttct caaaggttct
    12421 gattttgcta atttctgttc tcaaccgctg cagtaacaaa ggcagtgtag agttcttctg
    12481 gtcggtttgg acggcttgac agttcagggt gatactgaca agctacaaag aatttatttt
    12541 caggaatttc cacgatttct accaaacgat tgtctggaga aactcctgaa aagacaaagc
    12601 ctgctgcctc aaactgctca cggaaggcgt tattaaactc ataacggtga cggtgacggc
    12661 gttgcaccac ttcttgattg tgataagcag cagcagcctt agagccacgt ttcaacttag
    12721 acggataaag tcccaaacga agggttccac ccatatcctc aatatcaatc tgatcacgca
    12781 tgatatcaat gatagggtat tttgtttctg gtgcaagctc tgcagaattg gcaccttcaa
    12841 gacctaaaac gtgacgagca aactcgatac atgtcaactg cattcccaag cagactccca
    12901 acattggaac atcattttca cgcgcatagc ggatggcttg gattttccct tctgtaccac
    12961 gttgaccaaa accacctggt acgatgatcc cgtctgcatc agacaagagt tctgctacat
    13021 tctctgctgt cacatcattg gcattgaccc aattgatttt aacttctgca tcattgacat
    13081 agccagagtg tttcaaggct tcgaccactg agatataggc atcttgcaac tccacatact
    13141 taccaacaag ggaaatctta acttgtttct tgaggttcat gaccttgtcc accatggctg
    13201 accattctgt catatccgct gctggtgcgt ctaatttcaa atgatcacaa acaatttggt
    13261 ccatcccttg tgcctgcaag ttcagtggaa tttggtaaag atgttcaaca tccaacgatt
    13321 cgataacggc ttctggtgcc acatcacaga actgggccag tttattttta attccttgac
    13381 cagctggctc ttctgtacga ataaccaaca tatttggttg gattcccaag ccacgcaatt
    13441 ctttgacaga gtgttgggtt ggtttggttt tcatttcacc agcagccttg aggtaaggaa
    13501 gcaaggttgt atggatatac atgacattat ccgcacccac atctgccttc atctgacgaa
    13561 gagcctctag gaatggcaag gactcgatat ctcctactgt tccaccaacc tctgtgataa
    13621 tgacatcaga gtcggtcgtt agagcggcac gcttgatttt ttctttcaaa gcatctgtga
    13681 tatgaggaat gacttgaaca gttgccccaa ggtattctcc acggcgttct ttacgaagaa
    13741 cttcactgta aattttccca gttgtcacgt tggaatattt gttgagattg atatcgataa
    13801 aacgttcata gtgacccaag tccaaatctg tctcagctcc gtcatctgtc acaaaaactt
    13861 ccccgtgctg gtaaggactc atggttcccg gatcaatatt gatataaggg tcaaactttt
    13921 gaatggttac tttgagacca cgatttttca agagacggcc tagactcgct gccacaatcc
    13981 ctttcccaat ggacgatacc acaccaccag ttacaaaaat atatttcgta gacatagatt
    14041 cctctttcta aaatgctcaa ggtcttgtta actacgaggg gatatagaaa acaagaccct
    14101 actaataaga ataatctggg acgactgcac cagattcaca actaatactc ttcgaaaatc
    14161 tcttcaaacc gcgtcaacgt cgccttgccg tagatatgtg ttcctgactt tgtcagtctt
    14221 atctacaacc tcaaaacagt gttttgagca gcctacggct agtttcctag cttgctcttt
    14281 gattttcatt gagtataaaa tacaagaaga taacttcttg aaaaaacaaa aatagctccc
    14341 taagaactag ggagccccga cctctaaaag aggtgcccaa acaatatgat acctaaaaat
    14401 aggataattg tcaatagcta actcttattc ctcgctggtt tcagcgtcgt catctgaaaa
    14461 ctcgtcgtcg tcttcgttga gatccacatc ttctcccaag tcatctggag cgatttcgtt
    14521 gatttctgca tcataagctt ccacttcatt tttttcatca tctggatttt catcatcgta
    14581 tgaaagagct ggatctgctt cgtatgcatc ttcgtcttct ggatcatctg cattgtagtc
    14641 aatggcatct gaatcaccat ccataaaggc attgacacgt tttttcttag cttttggtgc
    14701 tacttcatcg tcgtcatttt cttcaagagc gatgatttct tcgtcgattt cgtccacacc
    14761 ataccatgaa cgaagacccc atttgttgtc cccaagtgag atgaagctac cgtcaaagtt
    14821 caactctgtg tagaacaaag gcaaagcttc gcggatatcg ctgtttgatg ttccaaggta
    14881 gttttgaatt tcgtttacaa gatcgctaaa atgcatctca tgatcgcgac cacgaagttc
    14941 caagatagca cgcgctacct caatcataga tagttcactt ttttcttgcc cagcaaatac
    15001 ttctaattcc aaagcgtttc tcctcattta tactactatc gccagagcga acagactctg
    15061 acctcatttt atcatttact ctttatttta cgataatttt gcggaatagt caaaggttaa
    15121 gggggagaaa gtggcaggat tagactaatt ccaatataaa actccttcct ttttctgttg
    15181 ctccattttc cacaaatcca agcgacttga aacacctcct agaagcatga ttgtaggtgt
    15241 agatttcctt gactctcaat tctttccatc cttttactcg agccaattca atcaaagcac
    15301 ttagaatctt ttttccaagt cctcgatgtt ggtaagcgga attcccaatc acaatgggga
    15361 gattatcctg agatagtgta atatccccaa ttggaaacca ttctcccttc tccttgactt
    15421 caatccaaaa aagctcacca tgccgatcca aataggaata catggcttcc aaggtcgctt
    15481 gactgtaagg aagcttcacc ccatctacga ggtaaaccaa gttcacatcc tgataccaag
    15541 caaaagcttc ctcacaatga ttgaccttat aataaggaac aaaacacaat gcactacctt
    15601 ttcaacaaaa gagttgctgc ttgattaaaa ccatcacacc agttatacca ttttgcttca
    15661 tacccatctt gagctaggat acgatcttct aaatcaaaaa cagagtaaat ctttctttcc
    15721 tcgcaagctt gcgcatagag atgatatagt tcatcaccac catctctatc ccactcagca
    15781 gaaatcgtat cccgacctgc caataaagcc tgataagccc tgtgatgccc atctgtaatc
    15841 agcaaacaat ctccaaaggc aagaatactg attggatcga cttggtttgt ttctgccgac
    15901 tggtaaagca tctgaatatc ttgcaacttc ttttctgata aatatagttg agtcggatga
    15961 agatctgcta tattgacttt catttctttc tcctcaaggg aattcgatac tcacttctgt
    16021 ttgcctttaa atcgccattg gaagcggagc ttgtcataga agggaaactc gataaacagg
    16081 actcccaagc ccacacagag actggcaaag acgtctgatg ggtaatgaac ccccagatag
    16141 actcttgata ccagcacact gactaggtag aggccaagga cgatttgtac gatttttctc
    16201 cagactggat ttttaattcg ttgactgaga atgacaatca gagtacctac catcaaagtt
    16261 acagccagag aatgtccact tgggaaggaa aatcccttct cttccaccag atgtaagata
    16321 gctggtcgtg ggcgctggta gatattttta aaggtcacga ttaaaagacc tgccaaagcc
    16381 agatttccca gcatgaagaa actttctatc ttccatcgct tacgataaaa gacaaaagct
    16441 gtaatgacaa cccaagtgat aatcactggg atatcaatca gacgtgtgag ggctcgaaaa
    16501 agaatagtca aataatctgg taagtctcct cgaatggcag tctgaatcga ttggtcaaaa
    16561 ttgaccaaca tttcagggta aaatttgacc atatagccaa gaataacgaa aagtaaaagg
    16621 gcaaaactgc ccttcattaa aaatgtttgt ttatctttca tagtgtttta aggttggttt
    16681 caaaaggacg tacaacaacc agaatgaaac ggaaaagatt acaccctcaa tcaagttaaa
    16741 aggtaatacc atggtcatta ggtagttgga aagtcccaaa atttttccaa tatcaaagtt
    16801 agcaaactta gcgtacaaag gaacagcata aacatagttg agaaccaaca tggccaaggt
    16861 taaaccaaca gttccagcta gagagcctag taggaaacga agggttgtcc gttccttttt
    16921 ccaaatcaaa gcaaatacga tgacaaaaac tcccaaagct acgatattca tcggcaaacc
    16981 aatgtaagta ttcactcctt ggctgttaag aagcaatttc aatagtgagc gaagcaagag
    17041 cactcctaga gcagcaggca aatccatgac caccagaccc acaaggactg gcaagatact
    17101 aaattcgatc ttgaggaaag atgccgctgg taaaagcgga aagtcaaagt acatcagcac
    17161 aaatgagatg gctgataaaa ttgcaatggt cgaaagtcga cgtgtgtttg tcataacagg
    17221 ttcctccaat tttctataaa atcagaagaa gttggaaagg attcctctat ctattctcac
    17281 tttttatatc ccaaaaattc cctcttactc tattaaagaa aaacaaagca agtggttaca
    17341 atccggctat aaatctatca aaacagacaa agctattctt tcgtcttctc ccatccagac
    17401 tatactgtcg gttgtggaat ctcaccacat cagcttgcgc tcgcggactt ctttaaagag
    17461 aaggaaaaga tcatttccta gttaaagtca ccgccggtcg ggaatcacac cctgccctga
    17521 agactctttt atcataacaa aaaacgcttg caagcgcaag cattttgttc attttcattt
    17581 tcatttttta tttcaattta tccacttcat aggtatggac gagctcaaga cctgcaaagt
    17641 tctgctggcg aagagcctca tagacaatca tgcagacggt attagacaca ttgagactgc
    17701 ggacatgttc atcattcatg ggaatacgga gagccttctc aggatgttct cgcataaaat
    17761 cttcaggtaa gcccttgtct tcacgtccaa agagaaaata atggtcctcg tcagtcgata
    17821 aatccacctc agaatagact ttttctgcga atttcgaaat cagatagagt tttcccttca
    17881 tctgagatat gaaatcttcc aaactctcgt aaaaataaat ctctagctta tcccagtaat
    17941 ccaatccagc ccgcttcatc ttgcggtcat caataggaaa gcccataggc ttgatgatgt
    18001 ggaggggaga attggtcgca gcgcaagtac gcgcgatatt gcctgtattt tgtggaattt
    18061 gaggttcaaa taatacaatg tgatttgtca tgacttgctt cctttcacca ttgcaaaaaa
    18121 atagccacac tgcccggagt caagctcagc aaacagcgtg gttaaggcat cgttaactta
    18181 cctcacaaca ggtttgaagt aaatcagcga aactactttc ttagtataac actttcagaa
    18241 tcattgtcaa tagaaatgac ttgatttttt caattttttc aagctatttc caagggttgt
    18301 aaaatcgtcc ctgattctgc aagataagta gtaaactaac tactaaaaac aaggttgcca
    18361 agagcaaggt aatatagtct ccttttttca aggcctgata actataccat gtgcgttttt
    18421 tctctttccc aaagcggcga agctccatgg cagtcgcaat ggtatcaatg cgttctagcg
    18481 agctaaaaat caagggagta ataatgagca gattgccttt gattcgttgc ataagagaag
    18541 ctttcttgga taattccatc ccacgcgctt cctgagacat cttgatggta aagaattctt
    18601 cctgcaaatc tggaatatag cgcaaggtta gactgacaga ataggcaatc ttgtaaggca
    18661 caccaatttg atttaaactg gaagcaaact gactagggtg ggttgtcatc aaaaagataa
    18721 tagccagagg aatagtgcaa aggtacttaa tagccaaatt tagcagataa aagagctctt
    18781 gactggtcag agtgtaggca ccgattccct gccaaatcac acttctctct ccgtaaagtc
    18841 caaccccata ctcgggagaa aagagataga ccatcaaaac gtttaaaacg gcaaataccg
    18901 tcgcaaaaac ggctacaaag gaaacatctt taaaatggat ttctgacaaa tagaggagaa
    18961 agaccgaaaa gatggcaatc agaacaagca gtctggtatc atagctaatc atggccgcca
    19021 atgacacgag aatgaaaaag aggagtttcc cagcgcctga caagcgatga atcacagtat
    19081 ctctatgctg gtaaccgatt aatttagctt gcatccttct ctcctttctt tgtaaaatgc
    19141 cgttaaagca agtggatcca cgtcgagttt cttagccaag ttgaagatag aagtctcttt
    19201 taggttggct tttactaaca gctcaggatt gctcaacaga ctagctggat cagtatcagc
    19261 aatcaattcc ccatccacca tgacaagggc tcgatctgaa taatccagca tcaattgcat
    19321 atcatgggta atcatgacaa tggtatgccc ttgttgatgc agttcttcga gaaattccat
    19381 aatctcagta tagttcttct ggtcttgacc cgcagtcggt tcatctagga ggataatttc
    19441 agcgcctaag accaaaattg aggcaatagt cacacgtttt ttctgaccaa atgacagggc
    19501 agaaatgggc caattacgga attcatagag accacagatt ttcaaggttt catagactct
    19561 cgtttcaatt tcctgctcgt ccacacctcg caaacggagt cccagagcca cctcatcaaa
    19621 aatcatattg gttgaaatca tttgattggg attttgcagc acatagccta ctcgttccgc
    19681 ccgctctgca acagaatctc cttttatatc ctgtccttcc caaagatagc ggccttcagt
    19741 ctggataaag ctactcaagg ccttggctag agttgacttc cctgctccat tttttccgac
    19801 aatggcaatc ttttcaccct ttttaatatc taaatgaaga gattttaaaa tcggtctatc
    19861 atcataagaa aacgacacgt cctctagtct aaagagtgac tgcaattctg gggtttcttt
    19921 taccagttcc gtccgcaact gaacctgacc ttttgagata gacaagttat ccagatctgc
    19981 taattgttct tccttgacta aatccacacc caattgacgg agagtggtga gataaagggg
    20041 ttcgcgaatt ccattttggg tcaacaaatc agtcgccagt aactggtcag ggctcccatt
    20101 aaaaaggata cgaccatcgt ttatcaagac aatccgatcc acagggcaat gcagaacatc
    20161 ctccaaacgg tgctcgataa taagggtcgt cgtcccctcc tccttatgaa tctggtcaat
    20221 caattcgata atatcctgac ctgacttggg atcaagattg gcgagtggct catcaaacaa
    20281 gagaatcgga ctttcatcaa tcaagacacc agccagactg actcgctgct tttgtccacc
    20341 tgacaaatcc tgagggcgct gatccagtaa aggaagaagg tccagctttt cagcccattt
    20401 ataaacacga cctttcatct catctagggc tgtcacatca ttttccagag caaacgccaa
    20461 atcttctgcc acagacaagc caataaactg cccatctgta tcctgcaaaa ctgtgctaac
    20521 cagatgagac ttatcataga tgctcatatc aaaggctgtt tgacccttta tcaaaaattc
    20581 tccatatgtc tgacccttgt aaatattggg aataatccca ttcaaacact gacccaaggt
    20641 agatttacct gatccagatg gtccaacaat taagactttc tctcccttgt aaatggtcaa
    20701 gtctatccct tgcaaggtcg gttcttgttg tgtttcatac cggaaagaga aatccttcca
    20761 ctcaattata gcttctttca tcttactctc ttcattcgct tcttagactt ctattttatc
    20821 ataaatcaag cccttcttgc agtctcttct cttaaaatct tagcgccaaa aagattccta
    20881 tcctagctta cttgcctaac taatctataa acatcgaaaa agactagttg cccagccttc
    20941 cccatcattt tatactcttc gaaaatctct tcaaaccacg tcagcttcgc cttgccgtag
    21001 gtatggttac tgacttcgtc agtttcatct acaacctcaa aaccatgttt tgagctgact
    21061 tcgtcagttc tatccacaat ttcaaaacac tgttttgagc aacctgcggc taacttccta
    21121 gtttgctctt tgattttcat tgagtattag tcctttttca aacttcctgc acgagtttgg
    21181 gttcctgcat aggcaagtaa gagaagagtt cctgcaatag ctacagatac accattggca
    21241 attcccgcaa caatcccttg tgcaaatact ttttctgccg cttcttgata aatcacaaca
    21301 tctccaagtg gtgccaagac accccaaaca agggcatttg caagtagttg aatgaggtta
    21361 aaaataagaa tatctttcca gtcaaaaaca ccattgatca cgcgaacgta ctttctaaaa
    21421 agtcccacaa ctaaaccaaa gagtccgcta gcgataatcc aagtccacca tagaccataa
    21481 ccagcaagag agtccttgat tgcatgacca atcaacccga caagcaaacc gataatcggt
    21541 ccaaaaataa tagaaagtag cgcttgtacc gcatactgaa gctggatgct tgtatttgga
    21601 acaggggttg gaatgttgat catcccgatg acgacaaaga gggcagcgcc aattccgaca
    21661 gcaacaactt gtttaattgt aaatttaatt tccatactat tctcctattt tatccttcta
    21721 ttttctttat ttcaatggtc caagatgaac cgacacctac attataggcc ttggcaaagg
    21781 aaccttggtt gatagccaaa cctaaacgat agagagagtt gatgtaaagg atgggttgcc
    21841 caattctcac atctgcaaat gatttgccat agacaacctg attttgatag accagcatat
    21901 cagcatgata gatggtcact tcaaaacgat caccaaattc tggttccagc ttgtaaaatt
    21961 cttcccgtgt gatagaggtc caaagcgaac cgaaacgcac atccagaata tcaatggctc
    22021 ccttcaccag atgatcttct atgatggtcg ctacgactgg aagctctaca atctgttcca
    22081 cactgagctc tggccctact tcctcaaaag taatgtgacc actggccagt ttagcaccag
    22141 tataggcata gacatcacga ccgtggaagg tataagaatg ctctgtgttt tgacgcctat
    22201 tggccacctc agaaatctca cgaatggcta caatgccaac gtgtttcttg ataaaggaaa
    22261 gcgtcccatt atctggcgtg acaatgtatt gattttttgc agtcttggca actacactct
    22321 tacgtttcga accgacacct ggatcgacaa ccgatacaaa cgtcgttccc tcaggccagt
    22381 aatccaccgt ctgaaagaga cgatagctcc cctcaaaaat attataaggc gtgatatcgt
    22441 gcgtcaagtg atggattttt aaggttggag actcttctaa agccactcca atcatagccg
    22501 ataccgcacc atcaaccaga ccaaagtctg attgtaatac cagtaaatta ttattcattt
    22561 tatctccttg tatatccttt atcataccat atttcagaga aagtcgctaa tagctatttc
    22621 aattggttag ggtatagttt taccttatag cgaattttca ctaaaagctc ttgttattag
    22681 ctagtaggtg cattttttct tgaaaaaggg tatgatagtt acatcatgaa aaagtaggtt
    22741 ttattatgaa aattatcctt gtcggagggg gaaaagttgg ttttgccctc tgtcgctcct
    22801 tggttgcaga aaagcatgat gttttgctga ttgagcaaga cgaagctgtt ctcaatcata
    22861 ttgtcagtcg ctttgatatc attggtatcc ttggtaacgg ggccgatttt gccattcttg
    22921 agcaagccag cgtccaagat tgtgatatct ttatcgccct gactgagcac gatgaagtca
    22981 acatgattgc agcagttcta gccaagaaaa tgggagctaa agaaactatc gttcgggtgc
    23041 ggaaccctga atattctaac tcttatttca aggaaaagaa tattctcggt ttttctctta
    23101 tcgttaatcc tgagctcttg gctgcccgtg ctatcgcgaa tatcattgac ttccccaacg
    23161 ccctgtctgt cgaacgcttt gctggtggac gcgttagcct catggaattt gtcgtcaagt
    23221 ccaccagcgg tctttgccaa atgcccattt ctgattttcg taaaaaattt ggtaatgtca
    23281 ttgtctgtgc gatagagagg gatcatcaaa ttatcattcc aagcggtgac atgactgtac
    23341 aggataaaga tagaatcttt gtcactggta accgtgtcga tatgatactc ttccataatt
    23401 attttaaatc acgcgccgtg aagagccttc tcatcgttgg ggcaggtaga attgcctatt
    23461 atctacttgg tattctcaaa gatagtcgta tcgatacaaa agtcattgaa atcaatcctg
    23521 aaatcgccag cttctttagc gagaaattcc caaatctcta catcgttcaa ggagatggaa
    23581 ccgcaaaaga tatcctgctg gaagaaagtg ctcaacacta tgatgccgtt gcgactctaa
    23641 caggagtcga tgaggaaaat ctgattacat ctatgttcct tgacagggta ggtgtacaga
    23701 aaaatattac taaggtcaat cgtaccagtc tcctcgagat tatcaatgcg cctgattttt
    23761 caagtatcat cacacctaaa agcatcgctg tagatacgat tatgcacttt attcgtggtc
    23821 gagttaatgc ccagtattca gaccttcaag ccatgcacca tctagccaat ggccaaattg
    23881 aaaccctgca attccatatc aaggaagcca ataaaatgac tgccaaacct ctttctcaac
    23941 tgaaattgaa aaaaggggtt cttattgcag ccattattcg aaagggcaag actattttcc
    24001 caactgggga ggatatgttg gaagttggag acaagctcct agtaacaacc ttgttgccaa
    24061 acatcaccaa gatttatgac ttgattgcga ggtaagaaat gaataaaagt atgattcgtt
    24121 acctcctttc aaagttactt ttgattgaag ctgttctcct cttggttcct gtggctatcg
    24181 ctgtctatta ccgtgaatcg agccaagtct ttacagccct cttttcgacc atagggattc
    24241 tcgtattgtt aggcggttca gggagtttac agaagccaaa aaatcaacgg atttatgcca
    24301 aggagggagt cttgatcgtt gccctctgtt ggatcctttg gtctttcttt ggtggtctcc
    24361 cctttgtctt ttctgggcaa atccccagcc ttattgatgc cttttttgag attagttccg
    24421 gctttacgac tactggagca agtattttga acgacgtttc agttctcagt cgttccctcc
    24481 tcttctggcg aagttttacc cacttgattg gagggatggg agtgcttgtt tttgcacttg
    24541 ctattatgga caatgccaaa aatagccacc tagaggtgat gaaggctgag gttccaggtc
    24601 ctgtttttgg caaggttgta tccaaactaa aaaacactgc ccagattctc tatctccttt
    24661 atctagctct cttctccctc tttgtcatca tctattatct agctggtatg cctctctacg
    24721 atagttttgt cattgctatg gggacagcgg gaactggagg ctttaccgtc tataacgatg
    24781 gaattgccca ctatggtagc tcactgatta cctatctggt tagtatagga gttttggttt
    24841 ttggggtaaa tttcaatctc tactactacc ttatgctccg tcgggttaag gccttctttg
    24901 gagatgaaga gcttcgggct tacttggtca tcgtgctggt ttctacaggc ttgattagcc
    24961 tcaatactct ctacctctac ccaggatttt ccaaaagctt tgaaatggcc ttcttccagg
    25021 tttccaacat cattacaaca actggttttg gatatggaga tattaccaac tggcccctct
    25081 tctcccagtt tatcctcctc ttcctcatga caatcggtgg ttctgctgga tcaactgcag
    25141 gtggactcaa gattattcga ggcctcatcc tttcaaaaat tgccaaaaat caaattttgt
    25201 ccattctatc tccccaccgt gttttgactc tccatgtgaa taaaacggtg attgataagg
    25261 atacccagca taaaattctc aagtactttg tcatctatgc tatgattttg ctatccctta
    25321 tctttattgt cagcctagat agcaatgatt ttctgatcgt gaccagcgct gtctttagct
    25381 gtttcaataa tatcgggcct attctaggaa ccacttctag tttctcaatc tttagtccta
    25441 tctcaaaaat tctcctctcc tttgcaatga ttgcaggccg cttggagatt tacccaatcc
    25501 tccttctctt tatgaagaga acttggtcta agagataaaa tacaaaatcc ctctttttac
    25561 aattgtgaaa gagggatttt tatattaaaa agggggaacg tttttaaatc caagcctgtc
    25621 tgtaagcaaa gtccttactc aaataaaaga tggaaaaact tactacacag cttggtaatg
    25681 ctccaataca aattgtaagg ctttacttgg attctttgtt aaatgaatat attccattcc
    25741 tctggtagca actatctgaa tacctaatct ttctgcatcc accttcatct ctctataata
    25801 actccgaact tgtggagcca gaataattaa atcataaaca cccataatat cataatgagc
    25861 tccgtacgct cctgaattcg caatcactct aacttctgct aattgagcgc cctcgttaat
    25921 tgcattggct aattgcgcac ttgttccaga ccctgcacaa agaaccagta ctttcaactc
    25981 cttacttttt atttcatcta actcaccagg cattatttca cttgtctcct cctctaaaat
    26041 aatatcattt gagcttgcaa catcttcttt catcagtaac tgtctatcat acgctctaca
    26101 gaatggcaaa taaatcaata tgtcgacaac taaaatcaaa gataaaaata caaaggagat
    26161 agattgaaaa ttcgttccaa ttaacaatcc caagggacca ggaaaggccc aaggtaactg
    26221 gatataaaat ccattcattc caaagaaatc aataaagacc tttcctagaa atacattcac
    26281 tggtggagtc atcaaaaaag gtacaaaaag atagggattc aaaataactg gcataccaaa
    26341 tagaagaggt tcattcaccg caaataaaac aggagtaatt gtagctttac ctaccgattt
    26401 taattgttta gaccgcataa agaaaatcaa aataaatggt acaacaaagg tagcccccgt
    26461 tcctccgata gcagcgatat aatttccgaa attaggcgtt aaagaatgat aaggaaactg
    26521 cccatttgca agaagttgag cattctcttc catattgctc agttgtaaag ctgtaacagc
    26581 aggtaagaca atagatggtc catgaattcc aacaaaccaa agcaaagcgc acataaacca
    26641 aatcaacatc atagcaggat atgattctgc ccccttaaaa ataggagtca atagttgttg
    26701 aaatacttgg gcaaaaggaa catctaaact aaaccgagat acaatatcta acaaaccact
    26761 aataagtaaa acaaaagaaa aaggaaaaat atctctaaaa gcttgtgata tagccccagg
    26821 aacttcctta ggtaaatgaa tagtaatgtc tcgcttaata cagaatcggt aaacatttac
    26881 agtaataaag gcactgacaa acgaagttat caatccttga gatcctaaat agtttgtcga
    26941 caaacttgta gatcccgtct tctcatcaac tacaagcgtt acagttaata ccagataact
    27001 acatatggct gccaacatta cagaagtatc atttattacc tttccaggag gcatttttct
    27061 attaacattt ccagccaatg actttgcaac tgtccctgaa acaataacac ccaaaactcc
    27121 catggttagc atatacactt tcctcatcca ggcaatagct ggactcggta actctactcc
    27181 gaaattttta ggaatcataa taataatcat caaaaagctt gaaaacataa ttaaaggcat
    27241 agtagcaatg aatccatctt taatagccat catataatta ttagtagaaa ttttttcaaa
    27301 tttcccttgt tgcttttcaa tggcagcgac tagcttatcc ataaaactcc tcctataaaa
    27361 aattcaacct catatttaaa acatattaaa catcattcaa atcgctaacc ttaattccgt
    27421 tttagccctt ttacccaaag agcacttttc ttaatatatc gcttctgtgt ctcataatca
    27481 acaaagaaaa gaccatatcg cttattgtag ccattcgccc aagaaaattg atcttgcaaa
    27541 gaccatataa aatatccttg aatattgact cccctatctc gagcctctaa aactttgtgt
    27601 aaatgttgct caatataacg gattctctta ctgtcatcaa taatatcatt ctcgccctct
    27661 ggcttaacct ctttaagggc tgtaccattt tcagttaaat aaatgacagg atgttgagga
    27721 tattcttctt tgatacgaag taacatatca aataagcctt gaggataaat attccaatcc
    27781 caatctgttg tcggaatacc aggctttttt acaaattcac ctagagcatt taatttaaaa
    27841 gaggactgtc cttttattcc tgttgaatta aattcttgtc tattttcacc tttgtattca
    27901 cgaataaatt ctgaacgata ataattcatc ccaaacatcg tattaagagg tgcagctctt
    27961 ttcataattt ctaaatcacc atcttcaata ataaagctag aattattagc ttccaatatt
    28021 tgatttaagt gaaaaagcgt gtcatcactg tagtagccca aaaaagttcc atctaataga
    28081 aatttattat tataaacatc ataccgttta gctgccaaaa tattttcttt ttgcccatca
    28141 ataggatagc ctggctttaa agcatgaata caacctacct ttccctcaat ccctaattga
    28201 tgaaattcga ggactgcaag agcatgcgcc aacaacatat tatgattcgc ttgaattgct
    28261 tcagataatt gaaaatgatg attcggagga aactgacctc ctatatattg acctgcagca
    28321 agagacatca gttcattgat tgtaaaccaa tgcttgactt ctgtaaattc ttggaaacaa
    28381 aagcgagcat atcgtatgaa acgatcaata ttctctctat tcaaccaatc ccctgtttct
    28441 aacattttct gaggcgaatc aaaatgatgt aaagaaacaa acggaatcac attatgtttt
    28501 aagcaggact gaaagactct atggtaatac tgaacagcca atatattcgg ctccccatct
    28561 atatcaggaa atatgcgaac ccaagaaata gataaacgca aagcctgcaa accatgttct
    28621 gccgccaagg ctatatcttc ttcgtaacga taataaaaat cactagctgg atctggtaag
    28681 aacggactat tttcttgcaa ataaacatcc cacatattta ttcctttgcc atctacccta
    28741 gttgcaccct ctacttgata agcagcggta gcagctccta ataaaaactc cttcggtaag
    28801 gctttaacct gtaaattttc catgactaat cctcattgca aaccaatatg ttcttcaatc
    28861 tttcgaattc gttcatattc atcaataaaa aactttacct gatcatacaa tagcatcgtt
    28921 gtcatcaaag tatcttgacc atgaatcatg ataaaactca cttcaaaatt atctccagat
    28981 gcctcctccg ctaataaatt agtctgttct cgatgggcag acactattga gtcgtttgca
    29041 gactcaacca attctcttgc ctgttcaaaa tatccatctc tcgcaaacgc caaagcatcc
    29101 atcaaatctg accttgcatc tcctgcgtaa gcgacaattg taaaaccaag catatttatt
    29161 tcttcttttg atttcatctt tcttcctcct caatcttgat acatctttta cacatcatta
    29221 ttaaaatgac ttcgctacga tacaaataga aagttgaccc taaatatctt aaaaatcctt
    29281 aaatttcata atatttagga tcaatcttct ctgaatacct tagttcattt tctatactta
    29341 taatttataa gtggaaacca gcgtaatttt ttgctacttc aattacacga taaacatcca
    29401 aattagattg gaatagcaat tcgtgaccac ggatataatt caaaacattt ctccattcat
    29461 cttgagcttg ccttgttttt ccagttgctt gaagatataa agccttcgcc atcttaacaa
    29521 catattcacg atgataactg agctgtttcc attcatcctt ttgactattt aataacttag
    29581 aaatattttc ctcaataatt ggtaaaaaat tatcagctag attgtaagct atataataat
    29641 gattcgctaa aacatcattt tgacggctgc caattgcatt aaaataatca catgaggaat
    29701 aactagataa tttttctaaa tattcaacaa cagactgcca atttgaccca tacaaagcag
    29761 aaaagtattc ttcaatcaat tcttcataac ttcttgtctt cttccagagc atttgcccca
    29821 taacataatt aggaaaatta tgagggaatc cggcacgtaa ttcttgacac gaaatgtacc
    29881 cgttcaaatg taggtttgaa agataagata catctttgta aatagtttga ctaattttca
    29941 tatagcctaa atcgccataa tgagcacgcc ctaaaggata gtcatatacg aaactatctc
    30001 ctttaaatgt tttttgccac tcaaaaagat aagataaatt ttcttcaaga gaattcggaa
    30061 gtataatttt attacgcaga taaggtttag gcgtaggtat ggaattgtca aaatctacat
    30121 ctgcataact catttcaaat gttcttgtaa tcggtgcaaa catcatggta aagcgttcag
    30181 gattatctaa tttttctttc tgaggcgccc ataacaactc atggtaaagt aggaaacaaa
    30241 tctttgtatc taatccctca ctcgttaaag ccctatccaa ttgattgaga atacgaatat
    30301 actgatccga aaccaattct tgtctacagt tttcgcattc acaaatatta ttacgagcat
    30361 ccgacaacca tacatgtaag tagttaacat caggtctttt cttggcataa tccttaataa
    30421 tttctaccat cttatcagct acatctggat ttgaaaaatc caggctggtt aaaatcggag
    30481 ccgtattaaa caattctcgt ttcccgttta tttcagcgac atagggtttc ttctcctctg
    30541 aaatactaag acctgattcc cagccaaatt ttgaagagta acctaaaacc tcacctgtcc
    30601 atccatgacc aacacgatga tgaataagac ctcttttttg caattcttta tccaacctat
    30661 cactcaattc ttgtactaat tcatttgaga atttttcttt atttaggtat ggattaaact
    30721 catgttcata ccaacgtttc aaaaaagaat agggattttc aaactgaatg aaaaaactgt
    30781 tcatcccaat cttaggtagc caatcaatga aatctagtat attttcaaat gaatctgctc
    30841 cctctataca tactccccta tgatagtaac ttgctgtttc atctatagat agctctttat
    30901 ctaaaaaatc ttcaaatctt aactctggaa taaagtcatg tccgcgccct ggtctagtat
    30961 acacaacacc aaattcatga aacattcggt aaattccaat aagtaaacct acaattgtat
    31021 tggactcaat tctcccctta cctttgttta atgagatgaa aatatggtct tttccatcct
    31081 cttcattgac taatcctaca aaaatatccg cttccttctc ctcgacaagt tctatatcgg
    31141 tattaaatac ctgattcata tagtatctta attcctctaa ggcaaagcta gaataagtat
    31201 ttttctgttg atcaaatact ctgataacca tcttatttcc ctccaacagt agctttttct
    31261 ttttcaagat tgcgtttatc tgcaattttg aagaatggat aataaattgc tacagaaacc
    31321 aaaatcaaaa caacttgtaa tagagctcct cgccaactag cccctgtcgc aaggaagcct
    31381 cctatgatag gtggcattgt ccacggaagg attacacctg ttgtataggg tactaagcct
    31441 acagccattg atacataggt aatcaaggca ttgattgtag gagtagcaat aaacggaatc
    31501 agcataatag gatttaaaac cgttggaaac gtaaatagaa tagctgtatt gatattaaag
    31561 atagacggta taatagctag cttacctaat gttttattag cacgactctt actaaatagg
    31621 aagagacaaa tcgcaagacc aatagtcgct ccaccgccac caataaatac aaataaatct
    31681 ttaaacggta atgtaataat gtgttgtagg gtttgtcctg cagccacagc ttcttggttt
    31741 tctgtagtaa attgtaacca aactgggtct acaaaagcat ttaaaacttg tcccccatta
    31801 actccacaga accaaaagaa tgagtttaca ataacacata aaatcatacc tggaagtgtt
    31861 cctgcaatta acttaagcgg cgttccaaca attgcaccta aaagtccgtt gagacctcct
    31921 gcaactcctg ctgcttccag accttttaag accaaagccc acaaaacaaa agtagtaaaa
    31981 ccaggtaaaa gagctgaaaa tgatttactg accacatctg ggacaccatt tggcattttt
    32041 atcgtaattc cgcgttggat aaaaatacga taaatctccg ctgtaaccaa tccaatggta
    32101 atcgccataa acaaagaaga cgcattcagg ctagaaaatg gtattgcacc tccaaataac
    32161 tgcttgacct gctccccatt tttatcataa accatacttg aaaaacgagg ggccatcaat
    32221 acaaaactgg ataaggctat gatccctgca gacggaccat ctgtaccata tccttccgac
    32281 aacctgtagg caataccgaa acaagcgacc aaggagataa tcgctactgt tgactgattc
    32341 atagttgtca agatacttag caatccaaca ctattcagcc aatttgtaaa agcctcttga
    32401 ggccaactga ttagaataag aaataaagag ccgactaaaa tcaaaggcat tgccatcata
    32461 aatccttcac gaatagcttt caaatgccgt tgattgccaa ctttttcacc aaacggagcg
    32521 actttcgttt ctaaaaacgt taaaatattg ttcatcaccg gaactccttt ttttattttt
    32581 tataaattaa atataccact aacagaaagc gttgtcaata tattttttac tgtttttaca
    32641 atattttttt ctttgtatag aataataatt ttaattgatt ttttcttatg tttaatataa
    32701 aataaaccaa aggaggaaac aaatgattac aaaacaatca gcctttttac aaaatagagc
    32761 cactatactg gaatttctat atcgaaatcc agcaacatcc cgtacagata ttgttaatga
    32821 aacaggactt actccagcaa caacgactaa catcataaaa gagctaagcg aacaatccct
    32881 catatacgaa actggtgatg aatttagcga gttttccgga tctggaagac gtagaaaaac
    32941 tatttctatt acagataata tcccatctgt tgttggaggt attgaaataa acgtcttagg
    33001 tatctttctc agtctatgtg acttacaagg gaaaactctt ttcgagacag aaattttgaa
    33061 tgaagattat cctatttcag aaatcaattc caccattacc aatatgataa aaacagctac
    33121 aaagtatgtc cctttggaga caaaattact tggatttggc ttatcaatac ctggacatta
    33181 taacaaagac tccggaagca tcattacaaa caaccccata tgggaatcat ttaatttatt
    33241 aaatgtaatt aaaaaattcg atttcccttt tattgtaaaa aataatatcg attgtatggc
    33301 tataggacaa taccttttta acccacacaa tacccccgat aactttattt tcctacacgc
    33361 tggattaggt atttacactt cctttttcac aaaagaaaaa ataggagcct ctaaaaatcc
    33421 ttatatcgga gaaattggac acaccattgt cgaattaaat gggcaatatt gtgaatgcgg
    33481 aaaaaaaggt tgtttacaaa catatatttc ggatgcttgg ttaatcaaac atgcccaatt
    33541 attatttaaa aattctcaac taactgtact aaaaagcctt gtaaagactg aaaaagacat
    33601 tcatttagat acccttttaa cagcttacaa cttaggcgac tctgctttac gccaacaaat
    33661 tgataaagga gtcaatttat tggccacttc tattgcaaat cttctcctca tcaatcctgc
    33721 tgataaaatc tatatcaaca gtcaattgct taattatcaa cctttcactc atgaagtcag
    33781 ggataaaatc caagatcagc tccacttcgt tccctttact cgtaatatag aaattgaaat
    33841 tttaccttac aacaaacatc gtggaagtat aggagcttgt gcattagcta tcgttgcttt
    33901 tttcatagag catagcaatg tattacaaga tattatttca ccttaatata ttagaaatct
    33961 atagacctgt ttaaatcaac tataacctgt agtaaatatc tcgtatttag acaatatgaa
    34021 aacaagacga cttccatata ggaaaccgcc ttctcgctat gctgcatgaa actgtaaaaa
    34081 tagatgaaag tgatagcggt agtcaaatca agaggcatca taactctaaa aagtcacaat
    34141 atataagttc atcctcagaa aaatatcatt ctaattattg aaatgcctac atgaaaagaa
    34201 acgttaaatg ctcataaaac aaggaataca ggtatcaaaa ctgtgacaaa acaagtccct
    34261 aaatttacta aagacactac tcaactttac acctgtaaat ggttgttgta taataaagtt
    34321 acaaagatgt acgaccacac tgttgtaaat catagtgttc gcgaatatat tactgatagc
    34381 atttctacaa atacaagtaa agggattgga tgagattcaa acgaaatatg tcagtgtttt
    34441 ggcattccta gccttcatat catttaaaga attgtataga taaaattttt cccaatacag
    34501 acactcgtaa caactgcttc atttttctac caacatattt aggaacagga taagatacaa
    34561 gaggatcaat ccataaatca gttctatacc aatctaagac aaataagcta aaaaaacgat
    34621 tgataataag caaatagatt ccaaattttc tctatctgct cattttaata aacaatacta
    34681 gtgtaactat ccttccagtc agaagcttgt caaatcacac cgaaaattct tctaaaattt
    34741 atctcgttag gcaatcaagc aaaaactcaa cgatagtaca aacattatca tacaggattg
    34801 acttcctaaa ttatatactt tagtaaggtt tttggataag aaaaaaggtt cattttacat
    34861 ttctaaacac tcttttctaa tacgagataa ttaaaaaaac tacattttta aatataaact
    34921 acaataaaat aaactaaatg ccataggaga aagacaatgg attggtacga ttatatgata
    34981 caggcatcca aacaatcaca attcaacgca agccattggt ttcgctattt gcgaaaagtt
    35041 atttttgaag actattctta tttaacaaac caagatatag aaaagttgct agactccaaa
    35101 gaactaaccc attttcaaaa aattagcttg aagtatgcct ttcaagagca tactccaact
    35161 cataaatatg tgatttcatt aaataaacct gctaagttaa ccaatgttca aaaattgatg
    35221 gagaaataca agcatggata aaatgaaacc ggtcttccaa gcactaaata aggaattaat
    35281 tcaggaaaat ctgactttaa caattatctg tgtcggtggt tatgtcttag aatatcatgg
    35341 tttgcgtgcc acacaagatg ttgatgcttt ttatgaccaa aatcagaaaa tcaatgaaat
    35401 tattgctcgt gtaggaaaac agtttaatct taacacccac gaagagttat ggctcaacaa
    35461 tcatgttgca aatatgaaca aacaacctcc cttaagttta tgcgaatcct tgtactcatt
    35521 tgagaatctg accgttttag tagtccctat tgagtatgtg ttaggaatga agatgatgag
    35581 tatcagagag caggatttga aagatattgg tgcaattatt aaatacaaaa actttcattc
    35641 accattcgat acctttaaat atttaaaaga catgggattt gatactattg acctctctgt
    35701 tctattagaa ggttttagtt atgcttatgg tatggattgg ttagaaaaat tttttaaaga
    35761 aaatcaagat aaacttcgcg aattttatta acagtatcaa acaagatgat tgtcaattag
    35821 ccagtcgtct tgtttttatg atgaaatgaa ataaaatcgg aacaaatcac ttagagaatt
    35881 ccagtcaatt tctagcactg tttgagacgt ttcagcggac tatagcaaaa tgaaataaga
    35941 ataggacaaa tcgatcagga cagtcaaatc gatttctaag aatgttttag aagtaaaggt
    36001 gtactattct actttcaatc tattatattt cagattcggt ccactattcc ttcccaaaaa
    36061 cattgcatat tgcgccgaag gaattgctca tactatccca agcacaaaac cctcagtcgt
    36121 ttgaccgagg gttccttttt attattcaag aacttgaggc tctataatat ttgtagtggg
    36181 taaatcccct atggatatta tggagcctat ttt
//
LOCUS       DAVKQB010000018        35057 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKQB010000018 DAVKQB010000000
VERSION     DAVKQB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35057
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.018"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(151..2256)
                     /locus_tag="VW528_000865"
     CDS             complement(151..2256)
                     /locus_tag="VW528_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9911711.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTVEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     TKHLEADMEDGVLVIREKA"
     gene            complement(2865..3845)
                     /locus_tag="VW528_000866"
     CDS             complement(2865..3845)
                     /locus_tag="VW528_000866"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9911712.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(3847..6099)
                     /gene="pbpX"
                     /locus_tag="VW528_000867"
     CDS             complement(3847..6099)
                     /gene="pbpX"
                     /locus_tag="VW528_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU9911713.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(6103..6420)
                     /gene="ftsL"
                     /locus_tag="VW528_000868"
     CDS             complement(6103..6420)
                     /gene="ftsL"
                     /locus_tag="VW528_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9911714.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(6432..7382)
                     /gene="rsmH"
                     /locus_tag="VW528_000869"
     CDS             complement(6432..7382)
                     /gene="rsmH"
                     /locus_tag="VW528_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9911715.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            7548..7742
                     /locus_tag="VW528_000870"
     CDS             7548..7742
                     /locus_tag="VW528_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9911716.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            7758..8300
                     /locus_tag="VW528_000871"
     CDS             7758..8300
                     /locus_tag="VW528_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU9911717.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNIIPLLLAYKYPQEVALIYPPLILVIALIASGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            8311..8553
                     /locus_tag="VW528_000872"
     CDS             8311..8553
                     /locus_tag="VW528_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911718.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            8564..8899
                     /locus_tag="VW528_000873"
     CDS             8564..8899
                     /locus_tag="VW528_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911719.1"
                     /translation="MKSLARLLIIHVFISIFLFFALTSGAISHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(9009..10010)
                     /locus_tag="VW528_000874"
     CDS             complement(9009..10010)
                     /locus_tag="VW528_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9911720.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(10070..11971)
                     /locus_tag="VW528_000875"
     CDS             complement(10070..11971)
                     /locus_tag="VW528_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9911721.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHYSKDYEKVKRSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKSYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIRVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(11993..12286)
                     /locus_tag="VW528_000876"
     CDS             complement(11993..12286)
                     /locus_tag="VW528_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9911722.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(12286..13104)
                     /locus_tag="VW528_000877"
     CDS             complement(12286..13104)
                     /locus_tag="VW528_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9911723.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(13091..13870)
                     /locus_tag="VW528_000878"
     CDS             complement(13091..13870)
                     /locus_tag="VW528_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9911724.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(13885..14376)
                     /locus_tag="VW528_000879"
     CDS             complement(13885..14376)
                     /locus_tag="VW528_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9911725.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(14387..15577)
                     /locus_tag="VW528_000880"
     CDS             complement(14387..15577)
                     /locus_tag="VW528_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEU9911726.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(15589..16023)
                     /locus_tag="VW528_000881"
     CDS             complement(15589..16023)
                     /locus_tag="VW528_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9911727.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            16296..17111
                     /locus_tag="VW528_000882"
     CDS             16296..17111
                     /locus_tag="VW528_000882"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9911728.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            17130..17771
                     /locus_tag="VW528_000883"
     CDS             17130..17771
                     /locus_tag="VW528_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9911729.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            17802..18803
                     /locus_tag="VW528_000884"
     CDS             17802..18803
                     /locus_tag="VW528_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9911730.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            18813..19442
                     /locus_tag="VW528_000885"
     CDS             18813..19442
                     /locus_tag="VW528_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9911731.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(19821..20206)
                     /gene="tnpA"
                     /locus_tag="VW528_000886"
                     /pseudo
     CDS             complement(19821..20206)
                     /gene="tnpA"
                     /locus_tag="VW528_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(20559..23638)
                     /locus_tag="VW528_000887"
                     /pseudo
     CDS             complement(20559..23638)
                     /locus_tag="VW528_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
     gene            23861..24337
                     /locus_tag="VW528_000888"
     CDS             23861..24337
                     /locus_tag="VW528_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9911732.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            complement(24559..26772)
                     /locus_tag="VW528_000889"
     CDS             complement(24559..26772)
                     /locus_tag="VW528_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9911733.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSKTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAIPLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNKNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQVAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GILDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPMYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLAFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTI
                     PVFAKSGAIIPLVGSEIDMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELTLQGDSSIVPSNRRHRIHFKGTNVSIIELPNKNDTARFECKDNKTISLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(27173..27349)
                     /locus_tag="VW528_000890"
     CDS             complement(27173..27349)
                     /locus_tag="VW528_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911734.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            complement(27729..29075)
                     /gene="celB"
                     /locus_tag="VW528_000891"
     CDS             complement(27729..29075)
                     /gene="celB"
                     /locus_tag="VW528_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEU9911735.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVIIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDVFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            complement(29155..29661)
                     /locus_tag="VW528_000892"
     CDS             complement(29155..29661)
                     /locus_tag="VW528_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911736.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            complement(29692..30006)
                     /locus_tag="VW528_000893"
     CDS             complement(29692..30006)
                     /locus_tag="VW528_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEU9911737.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            complement(30016..31995)
                     /locus_tag="VW528_000894"
     CDS             complement(30016..31995)
                     /locus_tag="VW528_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9911738.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNGFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            complement(32113..32427)
                     /locus_tag="VW528_000895"
     CDS             complement(32113..32427)
                     /locus_tag="VW528_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEU9911739.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            complement(32557..32795)
                     /locus_tag="VW528_000896"
                     /pseudo
     CDS             complement(32557..32795)
                     /locus_tag="VW528_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
     gene            complement(32847..34283)
                     /locus_tag="VW528_000897"
     CDS             complement(32847..34283)
                     /locus_tag="VW528_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9911740.1"
                     /translation="MTTFKDRFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITSGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            complement(34473..34811)
                     /locus_tag="VW528_000898"
     CDS             complement(34473..34811)
                     /locus_tag="VW528_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU9911741.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHYQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            complement(34967..>35057)
                     /locus_tag="VW528_000899"
     CDS             complement(34967..>35057)
                     /locus_tag="VW528_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9911742.1"
                     /translation="WAHIKKHLKKVLPSCHTFYEALLSCSCFN"
BASE COUNT        10621 a         7564 c         5812 g        11060 t
ORIGIN      
        1 tttttttcag ctggctcctt cttttttatc ctcaaaattc tatcttatac tcaatgaaaa
       61 tcaaagagca aactaggaaa ctagccgcag gctgtacttg agtacggcaa ggcgacgttg
      121 acgtggtttg aatttgattt tcgaagagtc ttaggctttc tcacgaataa ccaaaacgcc
      181 atcttccata tctgcttcca gatgtttagt atctaaatga tccaagtgga agtctgtcac
      241 cttatcacga atttcttgtt caaccacgcg acggagagga cgaaccccca tgacttcgtc
      301 gtaaccttct tctgtgatat aatctttagc cgcttgactg actaccaagt caatgtcttt
      361 cttagccaag gtttggttaa cttcagccaa catcaaatct acaatcttag aaaggtcttc
      421 cttagtcaat tgtgagaact cgatgactgc attaaagcgg ttgaggaatt ctggacggaa
      481 gaagggtttc aaacggtcca tcaattctgg tttatccgca tcttctgtca agttggcttc
      541 atagccaaat ccagcatttg aggtcgcaat aatgacagtg ttcttgaagt ttactgtatt
      601 tccttgacca tctgtcaaac gaccatcatc tagaacttgg aggagaaggg taataacttg
      661 aggatcagcc ttttcaattt catccaagag aatgatagag tatggattgc gacgaacacg
      721 ttctgttaag gtattgctat tgtcatcata acccacatag cctgctgttg taccaattag
      781 cttagaaaca gctgtgcggt cactgtattc agacatatcc aaacggataa tcgcatcctg
      841 ggttccaaac atatcgagtg ccaattgctt agcaagctcc gtcttaccaa ccccagtaga
      901 ccctacaaag aggaagctgc cgattgggcg atttccttca tcaaaaccag cacggttacg
      961 acggatagcg cgagctacag cttctacggc cttatcttga ccaatcacct tgtcttgcaa
     1021 gcgatgagcc atatctttca aacgttcgat atctgaagct cccatttgcg atactgggat
     1081 acctgtcatt cgttccacag attcagccac atcgttgaca cttgcagtca ctttcatatc
     1141 ttctgtgtgg ttttcgattt tcttttccaa ttctgcaatg cgtgttttat agtttagagc
     1201 tgcttcaaaa tcttctgctt caactgcttt ttcttgcttg tctttttccg tttcgatttc
     1261 tcgttcaaca gcatgcacat ctgttactgg atgttgagcc gccaagtgag cagccgttac
     1321 atcgacaagg tcaatagcct tatctggcaa gctacgttga ggaatgtatt gaacagaata
     1381 atccaccgct gctttcaaga cttcgtctgg caagatgaca ttgtggtgtt gttgatagag
     1441 gtcacgaatt ccttgaagaa ttttaaaagt attctctgcc gaaggagcat tgaccttcac
     1501 ttcgttgaaa cgacgagcaa gagcagcatt cttcaagatg gtgttacggt attcgtcttg
     1561 agttgttgcc ccaatcactg tcaattctcc acgagagaga gctggcttga gaatatccgc
     1621 aagtccttta gaaccactgt ctccaccagt gctaccagca ccaagaattt ggtgaatttc
     1681 atcaaagaag aggataatat tccctgcttc tttcacttca ttgactaagt tttggacatt
     1741 ttcttcaaag ctaccacggt attgagtacc agcctcaaga cctgagatat caatagaaat
     1801 aatttccttg ttcttgatag cagcaggaac atctccgttc acaatggctt gcgctagacc
     1861 ttcgacaact gctgtcttac caacacctgc atctccgacc aaaacaggat tgttcttggt
     1921 gcggcgtgag aggatttcag atgtttcttg aatttccttg tttcgtccga taacaggatc
     1981 caacttgccc tcacgcgctt ctactgttaa gtttcgacct agttttgcaa ggacaccgtc
     2041 ttgtttcata cctgatgcct gttgtggcat ttgcacatca gtttctgcat ttcctggtaa
     2101 ttgaccagtc gcacgatagt gagcaaattc ctcaggtgtg acttcgcgtc cattaattaa
     2161 gtaacggcga ttttcagaac tgtatcctcg cataccaccc atcaattggt taaataaatc
     2221 atccatgtta ttaaaattat taaagttgtt attcatattc tttacctctt tttgtttact
     2281 tagttatgat tactgatatt gactatcttt gaccattgtt ttaaaaaatt tagactagct
     2341 agatcgctac cctacgtact atttctggtt tttctttttc aagcaggagt agactatctt
     2401 tacttctgaa gatttctaga tgaaacatag acccacctct ttctatttta ctttaaataa
     2461 gtgttctagt aaggctttca tttaccttac gttcataata tactacattg gtcagaaaag
     2521 gtcaagagtt ttgactttaa ctgaccaata tttttttaca ctcttcgaaa acctcttcaa
     2581 accacatcag cttctcctta tctacaacct caaaaccgtg ttttgagctg acttcgtcag
     2641 ttctatcaac catctcaaaa ctgtatatta aaacgaaata agaacaggac aaatcaatca
     2701 ggacagtcaa atcgatttct aacaatattt tagaagtaga tgtgtactat tctagtttca
     2761 atctactata ttttgagcag cctaaagtta gctttctagt ttgttctttg attttcattg
     2821 tgtatttaaa cacaaaaaca ccctgaaaca tcagggtgcc attcttacat caaatataaa
     2881 attgctaggg tcaggagact tgctagaagt cccactcccc aaaagaagaa gtcaaccttc
     2941 cactcgctcc aaggatttcc tttaccagac aatcccccaa gctcaaaatg gtgatgtaca
     3001 ggcgtcatac ggaaaatacg tttaccacct gtcagtttga aataactgac ttgcatcata
     3061 acagaagttg tttcaaaaac atacacaatt ccgataatca agagagtcca ttcttggtgg
     3121 agagccatag agatagctgc cagcatccca cctagggcca aacttcccac atcacccata
     3181 aagaccttgg caggcttatg gttaaagatg aagaaaccga gcaaaccacc aatcatggca
     3241 agaatcacta gaagaatatc catctgacct tgcacatagg caataactcc ataggcagac
     3301 aaactaatca caacggaaat actagctaaa ccgtcaacac cgtctgtcaa gtttactgcg
     3361 tttgaaaaac cgactagcca gaaaagagcg aagaaaatat agaaaaatcc caaatgaact
     3421 ggataaccaa agacagacag gatatcgcca ccgcgctcat agaaaagata gaagataact
     3481 ccacctagaa gctgaagagc taatttttgc ttaggattaa gcccctcatt gattttacga
     3541 aagaccttga gaaagtcatc taaaaatccg accaagccat acaagaccaa gatgaacaaa
     3601 atcattccca cattattgct gaattggcta ctaaataggg cgaaaaagaa agcaaccaaa
     3661 acagaagtaa tcaagaaaac caaacctccc attgtaggag tcccagcttt tgcctgatgc
     3721 tgtttgacat cttcatgcat ctgctgtcct gtaatttgcg cctttctata aaattggata
     3781 aaggccggaa ttcctactaa agttagtaaa aatgtcacaa ttccagcact gatggaaata
     3841 aacatattag tctcctaaag ttaatgtaat ttttttaatg tccttgatag ctgtgttagc
     3901 acgaacatct tgcttctgca cagtagagcc cgaaccttga aattcaagtt ctatattgag
     3961 ccacttagca agggtctcag cagtctcctt tgtccaacca tacatatctg gaacttcctc
     4021 tgctttatca gataagataa ggacttgctg attcggggca agattcttcc cttcttcagc
     4081 agaactgttt ttaatcttcg ttcctgttcc cacaacgatg ggttgtacaa gattgcgacg
     4141 caattcttct gctaaatcac caggtgaaat atccttgaca ctaggcatag gataaggact
     4201 ttgttgactt acttgctcca aagctttagc tgttgtttga agattgagag agtctttcat
     4261 agctgaagcc cgctccaaga taggattggc aaattctccc aactgaatac ctgaataatg
     4321 ttcaggttgt tggaccgtca catacaagat aaaatcagga ttttcagccg gactcatcga
     4381 tacagccgag aaaatatagt cggttacccc gactagataa ccaccatttt tctcgtcagc
     4441 aatctgagcc gtaccagact tgagggctac attttgccca ggaacagtta cagttggctt
     4501 gcctgtgctg tggttataca tggttccata aaccggatcc gtccctacca aaaccatgtt
     4561 agtccgagtt agactagctg catctttaga aacaggattt cccacaattt ctttttgaga
     4621 tttccgagca gtttgatcat ttggatcata aatggcacta ataaatttag gctccagcat
     4681 gacaccgtca ttagcaatag ctgtaaaggc acgaatcatt tgcgtctggg tcactgaaat
     4741 cccttgtcca aatgagcttt gcgcaatgtt gacaatatta tccgcaggaa gctgaccagc
     4801 atactcatcc gtcaaaccga aacgggtcgg aactccaaat ttaaaacgat taagataatc
     4861 aagccaggta gcatctccca tcttttgctc aaggagggtc atcccaacgt tacttgagtg
     4921 tgcaaaacct tgagaaaaag tcatcattct gccaccagtc aatccttcat taacgtccca
     4981 atctcgaatc gtggcatctg caatttttaa ctcactacta ttaaagactt ctcctcctgg
     5041 aaaggtatta ttatcaatag cagcagccaa catcatcact ttcatagtgg aacctggctc
     5101 atagttactt tggtaaagga tatcacgcca aacaaagtcc tctgtaatgc cttcttttgt
     5161 atctgcatca aaggtcggtc gttgcgttgt tgccagaatt tcccctgttt tagcactgac
     5221 caaagtcgct gtcatgtact ttccttttac cttctcttga aaagcatcca tctgggtttc
     5281 cataaaggac tggagggggc tggaaatggt tgtataaaca tccttaccgt ccatcgttcg
     5341 ttgggaaact tgttctgttc cgggtacaat attacccaga cgatcctttt cataggtaat
     5401 aatgccgtct gtccctgcaa gaatactgtt caaggaactc tccattccag aggttcccag
     5461 caagctcttg cttccatctt cattttcatg gagctgagct aggccgataa aactagaagc
     5521 aaattgtccg tttgggtaac tacgattggg actggttgta aaatcaatcc ccttgacctc
     5581 tgcagcttcc aattcttttt tgatagacat catattggca taggtaatcc catttccctt
     5641 tgctccaaag gaaacttgct tgagattagg ttgcgagagt tgctctctta cataggattc
     5701 ttccatgtcc aaatacttat gaaagacctc tgcaaccttg ttaaattgtg ttttttctac
     5761 gtaaagaatc ttacccgttg ctgacttata gttctcatca atgaccgcat agacattata
     5821 ggaggttgca tcctcagcaa tcgggactcc atttcggtca taaatagtcc cacgtttggc
     5881 aggaactgta cgggtggttt gatgaacttt cttagcttcc ttcgctaaat ctgttccaaa
     5941 gcgagtgcct gtcccaataa tgaccgcaaa attgactaaa aaaatggcaa aaacaaagac
     6001 agataataaa ctcagacttt ttccaactct gcgtctgttt tcagccggcg atttccgatt
     6061 tttggtcgca taacggatta ctctttttgt ccacttcata tcttactccg ctattctaat
     6121 attttcattg tttaattgca aatcgtgtga attggcaatt tctttcaaac gttctgcacg
     6181 taatagttca ttgacctctt gcttggcatc gtccaattcg gttttctttt cctctatctg
     6241 cgcattgatt tttgtcaaat cattctgcac ttgcaagagc ttggtctgca taaaaataat
     6301 actaatggct acaataagag tggttacagc aatggaaaag taaaaagctt tttccacacg
     6361 cgaaaaccgt ttaagttgca tctgtagtat ttgacctgtt ttttccattt tttctgccat
     6421 ctttttccct cttacttgtg aatttttctg gccacgcgca acttggctga gtgcgagcgg
     6481 ttattggctt ctaactcttc cgcacttggc aagattggct tacgggacac caattccatc
     6541 ttgggcttga gatcatctgg gatgaaaggc aagccttttg gaacttcaac tgttgaagct
     6601 tccttgaaca attgcttggt caagcggtct tctaaggaat gaaaggtaat cactgaaatt
     6661 ctaccatcca gagccaacat atccatagcc tgctggatgg actcatctgc cgcccccagt
     6721 tcatcattga cttcaattcg aatagcctgg aaaatctgct tagcaggatg ccccttcttc
     6781 ttgagttcct tggcaggttt gaccaacttg ataatctctg ctaactcagt cgttgtctca
     6841 atcggcttca cttcacgcgc ttgctcaatc ttacgcgcaa tctgtttaga gaatttgtcc
     6901 tctccatact tgaagaaaat acgaaccaag tcatgatagt cataattgtt caccacttca
     6961 tacgctgtca gactagcatc ctgattcatc cgcatgtcca gtggcgcatc ctttttataa
     7021 gaaaaaccac gctcacgctg gtctaattga ggactagaca ctcccaagtc ataacaaatt
     7081 ccatcaattt cctgaacacc agcttcgcgc aaacgtgcct gtaaatgacg gaagttgtcc
     7141 ttgataaagg tcaccattcc cttctcaatg taaggtgcca agcgtttttg cgcattgtca
     7201 atggcattct gatcctggtc aaaggcatag agatggcctt tttcacttaa tttacttaat
     7261 aaatactcgc tatggcctgc tccacccaaa gtcgcatcaa cgtagatacc gtcaggtttt
     7321 acgtcaagca tatcaatcgt ttcgtggagt aagaccgtta catgatgaaa ttcttttgtc
     7381 atatcttatc tattttacca caaatccgac cagcttgcac ttgtcagaca aataggacaa
     7441 ggagactact atttcttcaa aaaatatgtc aaaaatactt gacactttat gtttaaaaga
     7501 ctataatata gtcaaatata tacgacataa atcagaaagg agaccagatg aatcgtgtga
     7561 aagaatttcg caaggaattg ggtatttccc agctcgaact cgccaaggat atcggtgtct
     7621 cgagacagac tatcaatatg attgaaaacg acaagtacaa tccaaccttg gaactctgtc
     7681 tcaatctcgc ccgcagcctc caaactgacc tcaacagtct cttttgggag gaagattttt
     7741 aaaaaaggag caaactcatg aaaaaagaaa ccttcactga aaaactgatc aaacgcacat
     7801 acggtatttc tggtcccctt gacgaataca aacggcgtga ggccgatagt attgggaacc
     7861 aagtctttat cgtcctcttt tatctgatga ttttcggaaa tattattcca ctccttctgg
     7921 cctataaata ccctcaagaa gtggctctaa tctatcctcc tctgatttta gtgattgccc
     7981 tcatcgcttc tggctatgtc acctaccaaa tgaaaaaaac aggcatcaca gtcattgaac
     8041 cagatatgct gaatgagaaa gaaagcaagc aactacacta ccctggtctg aaagcaggtt
     8101 tgttctttgg tctatggatg ttttttataa ctcctcttct cagtatactc atagatgaag
     8161 gtcaggacta ttttcattct cttctcacta taagaaatgg tgtatcaagt attctcggtt
     8221 ctattttctt cggagcgagc atacagttcc tcatctcccg tcgcattgca aaaactaaga
     8281 aaaatcaaga tgaggattag gaggaaactc atgaaaaaag aaaagaaaca gttacgttat
     8341 cctggtctga aagcaggttc gatttatgga acagtgatat tttttataat tccactcatt
     8401 gataccctaa caagtgaaaa tccaaatttt atcagttctc ttctaaatac aaaacatatt
     8461 tttaaaacta tactgggagc tttctttttc ggagtgatga tacatattgt cgactcactt
     8521 cgtattgcaa gagctaaaaa agaccaggat taggaggtgc cttatgaaat cactagctag
     8581 actactgatc attcatgttt ttatcagtat tttccttttc ttcgccctta cttcaggagc
     8641 tatttctcat acagttttac tactcctact cctctttctt cctgcgctca ataaaggact
     8701 tgagaaaata caatcaaaac ggatacctgt cctcaacgca gccctcttct ttctcctcat
     8761 atcctttcca caacttttaa ccaaccctgt ccaatggaaa ttttcaatat tcctagtcgt
     8821 aaccatcatt tcaagtttgg cctacttcta taacttttat caagtagtta aagaagtaga
     8881 tcaaaaacag ttgatttagg agcttgctcc cataatttct cattttatag gcaaataatc
     8941 ccacctagct tgttctaggt gggattattt tatttcttaa cagagattgc aagtcatttt
     9001 ccttcatttt agaaagtcga ctctttccaa ttcacactac aatccaagac ttgttgcctt
     9061 tcttcttgat tgcgaccttc aatctggtca atcaaaatct ttgtagcctg ttgtccttcc
     9121 tcaaaggagg gctgaaccag cgtcgaaaca cttggagaag aaaagcaagt ccactccgta
     9181 ttgtcaaaac caatcaaccc aacttgcggc aagttataat tcaactcttt gataacggta
     9241 aagactagag gtagggccca acagttaggg ataaatacca gagttttttc atcgggatcg
     9301 atttcttttt gtaaaaattc tttaatttgt tccaaattcg tatgcttatc ttcaatggtt
     9361 agactggcgt gacgcatatt agcatctgtt aaagcatcca caaaaccact tgcccgctca
     9421 atccgagtac tcaaacgact cgtatccgct gtaatcaaga gaaaatgttc ataacctttt
     9481 tcgatacagg actgggtcat gtcataaacg gcatcatagt tattggtttt aacccagcta
     9541 gtccggtgtt catagagctg actatcaaaa aagaccattt tcttcttttt ctcatcgatg
     9601 atacgagaat attttcggaa attagaggtc ggctgaataa taaagccgtc tactcccaag
     9661 agaagcatgc tttcaatata ccggtcctca ctctcttggc tgtaattact atttcctatc
     9721 attacctggt agccattctg gctggcgata tcctcaattc ccttaacaat ttggtttgag
     9781 aaactgttgg taatatcacc aatcaaaaca ccgattaatt ttgttcgttt ggagtttaag
     9841 ctacgcgcaa caatgctcgg tttgtaattt gtttcatgaa taactttttc aatcttttca
     9901 cgtgtctctt gggacatttt ttcatatttc ccgtttaggt aaaatgacac ggttgttttc
     9961 gaggtctgag ccatttccgc aatgtctttt atggtcagtt tcttctccaa tctaaaacac
    10021 ctcctactaa tacttattat aacatattca ctattcaaaa tgcctatttc tagcactgca
    10081 agcgaatcat tctctcattt attttatcat atactagaca ttttccacgc atttttgttc
    10141 cgtcaacgag gcacaatttt tcacctacac gaatatcctc gcttaataac agaatcagct
    10201 tgtctgtttc gtcatttttc acttcaaaag ccagagcatt ttccacttca cgatcatctg
    10261 tctggtagac agaatgacgg atgatttcaa aactctcatg cgcaatgatg gtataatcca
    10321 gcatctgatt ctcaaagaat tggcgcttgg tgagtttgct acttctttca agctcattgt
    10381 atttaggaga aatgatggta tcttccaaat caaaatcaac ttcactccat agtctcaact
    10441 gattgatttt cccatcttga taggtcacat ccttgtcaag gataaactga gtcaacgcct
    10501 catgctgacc ttgacacctg atgtcatcta ccaagagcca gacatcctct accaacatga
    10561 ggatttttct cttgtgaaga taaggcaaat caggttctgc tgaccaataa gccccttcaa
    10621 tataatgcac tccctccctt tctttatggt gacaaaacag ggagtgagga tagtattcat
    10681 attcccagga tcccgtgatt ctttccggag ctttcccatc tacaatgcag gtcgaatgac
    10741 tccaagcact ctttaagaga taacgttcat atatctcccg ataagaataa cgcccagcat
    10801 ctatgaaaat aggttggcct tgatactgta agcaaaaact attctcgtca ctatggctat
    10861 gggcacttcc tagcggacca tttttgaaaa atagataacg atgttcatcc ttaatgcaga
    10921 catgtccaga gtcttcaaag atcatggact taggctgcca agctctcctt tcaaattcct
    10981 gcagtcgctt gaccttttct cgccccagga acaagaggct aagcaaatcg actttaacat
    11041 ccagaccgtt aagaaggtct tccttgttca aaaccacagc agacagactc aaaatttctg
    11101 tcgtttctgt agaatcgcta tcaccaaaag ccaaagttcg tccatctaag cctgtcatca
    11161 tttgaatata ggtcgccatc ttttccagca actctcggta actatcttgc aagtctggaa
    11221 gcaagagaca caaatccagc aaggctttat aaacctctac atgatagaga atcgactgtt
    11281 caaactggct tccatctcct aaaatctgtg tctcaatttg ctgtttcaac tcctctgaag
    11341 caaaatggta agcttcttct agatccatct tatctgaaaa gaaataatag atagcaagca
    11401 tcggaattgt ttgtaaaatc ccccagttac taagggtgta cttggcgcga tagtggcttt
    11461 tcataaagtc aatctgcttt tctagactga ccaaaatttt ctctagttct ttctcctcta
    11521 gcaagtcaaa tttcaagagg agcaagagta gtttcaacca agtaaaggaa cgaatacccg
    11581 tatccaaggt tctagtcatc aaggattgag gagaaaattc tctcacctgc tcaatccaat
    11641 caaatagaaa gaacttgcac ttttgaatat agctcttatc tccttctacc acatacccta
    11701 tcataaactg caagagatat tcttgtcgat tgagcatata agcccattct ggatcatctt
    11761 caaatacttg atcccatacc atcggctgga tttgatggat ttttgaacaa ggctccatat
    11821 cccaaggact atcaaacata aaacgattgt ccatcaagcg ttcaagggaa cgcttgactt
    11881 tctcatagtc ttttgaatag tgggacaaga tataatcacg acattggttt ccatcgattc
    11941 tttcaaaaaa ttgtcttctt tcttctttca ttatctatta ccagaaaaag aactacttaa
    12001 aaagcagttc ttttgtcttt cccattacac tttccttttc tacatagatg accacacctt
    12061 ttgcaatctg caaggagacc aagtcatctt tgatagaaat gatttttcca tgaattccag
    12121 acaataacaa cacttcatca ccagatgtta aagaagctaa atactcttgt cgttgctcca
    12181 tctgtttgcg aagcaacttt tgctgacgaa tagaatgaaa gcttgacagt aaaaggggac
    12241 tcactgccaa gacaatcact attccataaa acaatgttgt atccattaag ctataatctt
    12301 aagccagctt ccgataattc cgatgataac tgttaaaata acgagtttat atgttgtcca
    12361 tttcttttct ttgatcaagt agtaaactaa aagtgtaaat agggctggta gaagagctgg
    12421 agcaacctta tcaagcattc cctgaatact tacgatactt tgtttagcgt ctgctttaac
    12481 ttcccctgca gcaaaggtaa tcggcaccat aatcttaaca gatgtcgctg ccaaaccagc
    12541 aattacggtt acaccgataa tattggcaat acgagaaatc gttgccatct gctcgcttag
    12601 tttatcaatc acagttgttc ctagtttgta tccatacaga ccagttgaca atttaatcgc
    12661 tgttaaaatc gtattcatcg caaggaagaa caagattgga ccgacaacca agccttcttg
    12721 agcaaacgaa gctgcgatgg ttgagaacaa tggagctaaa cagaattgag aaagagaatc
    12781 cccaatacct gccaatggtc ccatcaaggc catcttgatg ctacgtgttt cttttgttgg
    12841 tcgaccattt tccaacatta caagatgcaa gctggtaata aaaggcagga agtgtgggtt
    12901 ggtattatag aattcacagt tttcttccaa ggcttggtag aaaccttcct gatcctctcc
    12961 atagtgtttt ttcaaagcag gatacatcac attggcatat cccaaccctt gatagttact
    13021 atagttaaat ccattttgac aaaagaatgc ccgcaaagac gttttaagat aatcacgttt
    13081 tgttaatttg ttagatccag tcatcgtgtg cttcctcctc taccacatga tccgctgttt
    13141 ttggcttgtt ataaaattca atcaaagcaa agatagtacc tacaattgca ataccaattg
    13201 ttgggatgtt tagataagct gcacaaacat atcccaacaa gacaaaggga atcaactctt
    13261 tcttagccat cactgacaag atcatcgcaa aaccgatagc tgggagcatt ttaccagcaa
    13321 ctgtcaaacc tgtaagtaat accggtggaa tgtagtctac gagtttcaac aaggtatcca
    13381 ttgaaagggc acccaagcaa cccaaggtaa atccaataaa ggcaaacaac caaattgttg
    13441 catttagagt gaacttaaat ttcttcaaat tatggttttt caagtgcttc atcgctgttt
    13501 caggcgcacc agcacgtaca gtgtaggcga aagtttgtaa gaattgaatc gccacagcaa
    13561 tcggagtaga gagggcaaga gccgcttctg gactgacttt accagcacta gtgatagcca
    13621 tcaaggtacc aaagatacca ggtccgattg ggtttggtgg aacagtacct ccagcaccta
    13681 caccgaatcc catataagcc aattcaccaa tagctcccat tgcaagagca gtaggcagat
    13741 cacctagaat aattccgaca ccaaatgaca gaacaataca tctattggtg taaattccta
    13801 acagcattcc actaaaacag aaagctgtcc ataatccaat taaaatcgct tgaaatacat
    13861 taatcgacat aacgatctcc tcttttaaat atagtccata atgttgactt gaacagctcc
    13921 atcatttcct gttggagttg ttttcgtatt aaatgttact tgatgagttt ggctcaattc
    13981 cttgagggct gccttgtcct cttcacccag gaagatggag cgtgtcactt gctctttacc
    14041 aggggcattg tgaatgttcc caatattgat ttctttgata gggacaccac cttctaccaa
    14101 ggttaaagcg tcctttacat cctttacaac gataaagatc gtttgagcag gattagcctt
    14161 gtgaatgata tcaatcacct tttgcaaagg gaagaaacgc atggcaactg agtctggcac
    14221 aactgttttc atcagagttt gttgcatctt gtccgtgctt acttcgtcat tggcaacaat
    14281 gaccgtatta caacctaggt attttaccca aagttgtcct tgtccatgaa tcaaccgttc
    14341 atcgatacgg gtcataataa tatttggcat tgtcatattt ctcctcctac caatataggt
    14401 tccagtcttt gtagaaacgg ataagggctt ctaggtaata gtagtcaccc cagatattgc
    14461 cttcatccac tcctttacct gaatgccatg agtacacacc gtggaggaga cttgtcccac
    14521 caggggtaaa ttgatcattt gcataatgtt cgatcaagga acgaagcatg gcatgcatag
    14581 catgtttata aatatctttg tcagcatcca cctctgggag atgttttagc atttcatgaa
    14641 tcccacagac ggcgatagct gttgctgaag aatctcgtga ttgatcacta ccatcattaa
    14701 aaatcaaatc ccaataggac acatgatctt ttggcagacg attcaagaaa taattggtca
    14761 cacccttaaa caagtcaaag caggactcgt cttttaagtg acgataagtc aaaggaatac
    14821 catagactcc ccatgattga ccacgtgccc agcatgaatc atcactatac ccttgtctcg
    14881 ttacaccttt aaagggttga cctgtctcag gatcaaaata gaaggtgtgg aaggacgaag
    14941 cgtcatcacg gattacatta ttagctgaag catagaaatg actttctgca atatcgtagt
    15001 atttttgatc acctgtttct tgataagcaa agaataagag ttggatattg agcaagcagt
    15061 cgataatcaa acggtaatgc tctttcttgc ccaagtctcc ccaagcttga ataaaaccac
    15121 ctttttcttg atagcgttca atcaacttat ctgcagcttt caaggttgct tctctagcct
    15181 ctccatctcc atttatctta tattcagcca tacaagacgg tgtgtacaag aagccgagat
    15241 catggtgatc caattctact ctcttattga cacgatccag gaaagaaaga acatttttat
    15301 gagcgatgtt tttaaatgca tcctgttgac tgtattcata agccaaccac agttctcctg
    15361 tccagaaacc attggtccat tccgtgttat ccatgattgg atagacatta tcaaaggtag
    15421 ctggcgtcgg gaaatcttcc ttgaaatagt caaggttgag ttctaactgc cgaataacct
    15481 tatcgattgc ctggccgact tcttctttcg tcagaagtgg tacttctaag aagcgctcag
    15541 gcgattttat tttttcaatc gtaacctttt ttatcattct tttacccttt aaatacccga
    15601 ttcgaaatct tcttcctctt cctctgcatc cgttgaaaac aattctttac catttacgac
    15661 tccgccctgg gccgccacta ctgatttatt aacaacctca tcaaagctat gagccattct
    15721 agcaaagact gcttccatta acatggctaa gttcaaaccc gagagaacat tcattgtttt
    15781 ggctggattt tctcccatta tggtagaaga aaccttgaat ggcgatcctc ccaagagatc
    15841 acttaggatt aaaacttctt cttcatttga aattgcaagt aagatttttt gcttgagttc
    15901 atctgctgac attccttcca caaagtcaat cgcctccaca ttttcttgat tgcctgcaat
    15961 caattgtaaa gaactataaa tccctgtagc aaaatgtcca tgccctacaa gtacaatttt
    16021 catcttttct cctttcttgt ttaggtttac cggtttactt tatattcccc attgtaatcc
    16081 tttattttta tcttgtcaat agttttttaa aaaaaaatat tagcgctttc attatctttt
    16141 taaccatttt aataggcaga aatttcaaaa tcttccgttt ttttaggaaa acaaaccttt
    16201 ttataaataa aaatatttat ttattgacaa aaccggttta ctagaatata atttggttat
    16261 agagaagtct taacaaattt gaaaggaagt atcaaatgac aaatacatca ttctcaattg
    16321 agcagttttc tttaaaagga aaaattgctc tcatcaccgg cgcttcttat ggaattggat
    16381 ttgctattgc caaatcctac gctgaagccg gcgctactat tgtctttaac gatatcaatc
    16441 aagatctggt caataaaggg attgaagctt atcgtgaagt tggcatcgaa gcccatggat
    16501 atgtctgtga cgtgacagac gaggacggta tccaagccat ggtcaagcaa atcgaacaag
    16561 aggttggtgt cattgacatc ctcgttaata acgctggtat tatccgccga gttccaatgt
    16621 gcgaaatgag cgccgctgat ttccgtaagg tcatcgatat tgacttaaac gcaccattta
    16681 tcgtttcaaa ggcagttatt ccttctatga taaagaaagg gcatggaaag attatcaata
    16741 tttgttcgat gatgagcgaa ctgggacgtg aaacagttag cgcttatgct gctgctaaag
    16801 ggggcttgaa aatgttgacc cgcaacattg cgtctgaata cggtggagcc aatatccaat
    16861 gtaacggaat tggaccgggt tatattgcca ctcctcaaac agcacctctt cgtgagttgc
    16921 aagaagatgg ttctcgccac ccatttgacc agttcatcat tgcaaaaaca cctgctgcac
    16981 gttggggaaa tcctgaagat ttgatgggcc ctgctgtctt tctcgctagt gatgccagca
    17041 attttgtcaa tggccacatc ctatatgtag atggcggtat cttagcctac atcggaaaac
    17101 aacctgagta aaaatagaaa gaagatctta tgaaaatcgc attaatcaat gaaaatagtc
    17161 aagctagcaa gaatcacatt atttacgata gtctaaaaga agcgacagat aaaaaagact
    17221 accaattatt taactatggt atgcgtggag aagaaggaga aagtcaatta acttatgtgc
    17281 agaacggact aatggctgcc atccttttaa atacaaaggc agttgacttt gttgttaccg
    17341 gctgtggtac gggtgtaggg gctatgcttg ctttaaacag cttccctggt gttgtctgtg
    17401 gtctagcagt ggacccaact gacgcttacc tttattctca aatcaatggt ggtaacgcct
    17461 tgtctatccc ttatgccaaa ggatttggct ggggggcaga actgaccctc aaattgatgt
    17521 ttgaacgctt atttgctgaa gaaatgggcg gtggctaccc aagagaacgt gtaatccctg
    17581 aacaacgtaa cgctcgtatc ttaaacgagg tgaaacaaat cacccacaat gatttgatga
    17641 ccatccttaa aacaatcgac caagacttcc tcaaagacac catctctggc aaatacttcc
    17701 aagaatactt ctttgaaaac tgccaagatg atgaagtcgc tgcttatttg aaagaagtat
    17761 tagccaagta aagctattct aaaccagaaa ggaactaatg gatgacgaaa atattactgt
    17821 ttggcgaacc attaattcga atctcaccat tagatgccac cagtatcggc gatcatgttg
    17881 ccagttcgac ttattttggc ggatcagaaa ttaacatcgc ttgtaatttg caagccctgg
    17941 gtatctcaac gaaagtcttt accgcactcc ctgccaacga gattggagat cgttttctca
    18001 cattcttgaa acagcaccaa atcgatacca gttcaatctg tcggcttggc gatcgaatcg
    18061 gcctctacta tttggagaac ggctttggtt gtcgtcaaag tgaagttttc tacgatcgta
    18121 agcatacgag tatcagccag attcggccaa acatgctaga tatggattct ctctttcagg
    18181 ggattagcca ttttcatttt agtggaatca ccatagctat cggtcaagag gtccgtgcga
    18241 tccttctcct actcttggaa gaagccaagc gccgaggaat tgtcgtttca atggatctca
    18301 atctgagaac aaagatgatt tcagtcctag aagccaagta tgaattttct aagtttgcac
    18361 gttttactga ctattgcttc ggtattgatc ctctcatgat tgatgaccaa aatctagaga
    18421 tgtttccaag agacagtgct agcctagaag aggtggaaaa tcgcatgcga cttttaaaag
    18481 aagcctatgg tttcaaggcc attttccata ccctccgatc tagtgatgag caagacaaaa
    18541 atgtctatca agcctatgct ctggaagaac gatttgaaga gtctgtccaa ctaaaaactg
    18601 cagtctatca acgaattggt agcggggatg cctttatatc tggtgccctt taccaactac
    18661 tccatcattc ctccctaaaa actaccattg actttgcagt tgcgagcgca actctcaaat
    18721 gcactcttcc aggagaccat ctctccactt ccgcaactag tattgaaaat ttactggcaa
    18781 atgcacaaga tatcattcgt taggagaatt acatgaccaa atcagatacg attattgaac
    18841 taaaaaaaca gaaaattgtc gctgtcattc gaggaaatac aaaggaagaa ggactacaag
    18901 cctcgattgc ttgtatcaag ggcggtatca aagctattga aatcgcctat accaatcagt
    18961 atgcaggaca aatcatcaag gaacttgtag acttgtatca ggacgatcag agtgtttgta
    19021 tcggtgcagg tactgtgctt gatgccgtaa ctgctagaga tgccattcta gctggagcaa
    19081 attacgttgt ttctccatct ttccatgctg aaactgcgaa aatgtgcaat ctctacagca
    19141 caccgtacat tccaggctgt attaccctca cagagatcac gactgcactt gaagccggta
    19201 gtgaaatcat caaactcttc ccaggtagta ctctcagtcc agcatatatc tctgcagtca
    19261 aggcaccgat cccacaagtt tccgtaatgg taaccggagg agtcggccta aacaacatcc
    19321 ctcaatggtt cgctgctggt gcagatgccg ttggaattgg tggcgaactc aataaactcg
    19381 cttcccaagg caactttgac cgcatcagcg agattgccca acagtatatt acactcagat
    19441 aaaatcataa ctacccgtct aacgggtggt ttatctcaga gctataagcc caaatcatca
    19501 gccagcgcct aaagacgctg gctttcacgt tgttcaagcc ttattgctct tgactcgtca
    19561 cttgcctctt taagagactt tggtattact taccactatc cctaaaggga tcctcatatt
    19621 cttttacact caatttatct agtgctatag tagattgaaa ctggaatagt atacctctac
    19681 ttctaaaaca ttgttaaaaa tcgatttgac tgtcctgatc gattttgtcc tgttcttatt
    19741 tcattttact atatatcata ctttactagt tctcaaattt tcatactcgt gaagaaatca
    19801 tccactcgat aatttcttta atcttgacta tatttcttaa ttgtggcttc attaagccct
    19861 actggactta cataataacc ttccgcccag aaattccgat tcccaaactt gtacttgaga
    19921 ttggcgtgtt tgtcaaacat catgagtgca cttttgcctt ttaaataccc cataaaactt
    19981 gaaacactta gcctcgacgg aatactgact aacatgtgta catggtctgg cattaagtga
    20041 ccctcgatca tttcaacacc tttataacta cacaagcgat gaaatatttc gcctaaacta
    20101 cttctatatt gattatagat gacttttcgt ctatacttag gggtgaacac aatatgatag
    20161 aacacatcca ctttgtgtat gataaactat gagtcttttg tgccatattt tttctccttt
    20221 cgctttacaa ttggattgaa tacctttatt gtatcgcgtt tggagttttt ttggtataac
    20281 cttcgacgcg cacctcacgg agcgagacgg acttatatag tggagtgaaa taggatacga
    20341 acaaattgat taggaaaatc aaatgaattt atagaaatct tttagcagtt ataacgttct
    20401 attctagttt caaaacgcta tagtcacata ataatgaagt aaaaaaggat aagtatcaac
    20461 ttatcctttt ttaaaagaaa aatccgaaga tatttggcct tcttcggatt ttttctattt
    20521 tccacagttt catgtaattc atctagatga tgaacaaatt agttgttctt tcctctacgg
    20581 aatagataaa atgccccaag tagcaagaac cctagacttg ccaagattga ctgaccttct
    20641 cctgtctgag ggagattctt ttgatccgaa tggttctttt cctcttcaga tttttccttt
    20701 tcttttgaat tctgtacttg tggctgagct gcttgctcta gctttttaaa gacttcctga
    20761 tctggagctg attcctgggt ttcaggatta tagtaggcaa tcttatattc atccccttct
    20821 tttcgaatgg tatagactcc acgtttcaaa acttggaatt ggttggaaat agtagagaca
    20881 gaatcatcat atttcacaat gccccaaact ccttgtttag catcataaac agactgaagg
    20941 gtttcgctat tttcgatgag gctactttct aactctttta tcatttgatt gaaggtggca
    21001 cgatccacgt taggaattag catatagcca taagaatctc cattttgctt atgagcctga
    21061 ctaatcgtaa gaaattcatt ttcaacttcc ttgtctgact gtccttcatt gatatccttc
    21121 caggctccct tttgcaaagc cttactcata ctgattgaac tcttcttaaa gaaaaagtaa
    21181 ccaatattct ttttcgaatc ggacgattct aaaaagacac tttgggtttc aggataatcc
    21241 ttttcttgtt ctgtaaggga ggcttcttta tcattgacat agactttata tggattactt
    21301 gattccagtt ggtgaaatcc atggttgctg tcgcattggc atcatctagt ttgctcgttc
    21361 caacgaaagc agacggtaga actttacctg tatcgctatc cgctctctta gcatccgtct
    21421 ctgttgtacc aggcatctta tatggattaa ctgttggcca gtagccatcg ctatagtgac
    21481 tcaaatcgcc attgtaaaga tagaacatcc catcactcgt ataccaacca tgtttatttt
    21541 ccttgttcat gtgttcgtaa ttcaaggtac gactggaaaa gagtgacaag ccaaatccaa
    21601 accctttctc tgcattgtac atggctgttt tatccatctt gttaaaggca gataggtaac
    21661 ttgttcttgg aacacttgcg actcctgcat cacttaacaa ggattgcatc aaactgatat
    21721 ccttataagt cttcaaattc ttaaagacat cataataact atccgattga acaatggtct
    21781 tcacaagact ctgcaaacgt tgtttggttt ctccttcaga catatccgct attcggtgaa
    21841 tccctcttag tacttctact gcggccacgt gcccctcgct atttgcacga ctgatcgagc
    21901 gtccacgact catatccatc agctctccat tcaccagcaa aggagcaaac gatttatcaa
    21961 tccagtggta catggtttgc attttatctt tatcgattgg attcttggtc ttttgaatga
    22021 ctggcaacag ttgagacagg ccatcaatca aaacattccc ataagcaccc gtataggcaa
    22081 cattggtatg gtcgatatag gatccatctt gataaaaacc ttcaccttgg tctaccaact
    22141 tgaacacttg ctcaatcgag cgaatggtag aagaaatttc ttgatcatcc ttacgcagta
    22201 aaccagctat tacttttact cttcccatat caactaagtt tccacctaga gccttaaatg
    22261 ggttatcagt cgtctttcgg aaatgttcgg gatctggtac aaatttttca atcacatctg
    22321 tatatttttt aatttcctca tcagagaagt attctttcat cagagacaag gtattgttga
    22381 tggcacgagg tgtaccgatt tcataatccc accagttccc aacaatgctc ttttcactat
    22441 tgtagacatg tttatgcatc cattccatgg aatccctgac tgttcgaacg acagtttcat
    22501 cttgataata acgagaagaa ggattggtca cttgcttggc catctcctcc aatttccgat
    22561 aagtggcagt cagatttgca gacattttat aatttgaaaa tttttcccac aaataggtgc
    22621 ggtccgcctg acttgaaata ctagataggc tatcagctac ctttccttcc aattcctggt
    22681 ttaatttggc catctgttca tttttagaat catagtattg attcccagca atgatgccat
    22741 tccagtcatc caaacggtct gtgtatgcat ccttaacaga ggctagaatc ttcaaaggaa
    22801 tctttttcac ttccttgcca tctttactga caatgacatt ggttgtccct tccttaagag
    22861 gttctaaaat tccatttttg actgaagcaa cgtcaggatt ttctacctta taagtatagt
    22921 ccgcaagaga aaaaacatgt ttttttccaa ttggtaaatc aatcttttcc tcaagctgtt
    22981 tatctgtttg agaatcctca gaaagctggg ctgctacctc taccagctca atatccttaa
    23041 aggaaacagt cccagttcct gtttcataga ataactccag cttgatttta tcaacatcta
    23101 aagtcgggct atagtctgct tcaatggtct gccagtcctt tgttcctgac gtcgttgcag
    23161 aattccacaa tcgcttgtcc ttaccacttt cctcaatgat acgaactttg gcaatcccga
    23221 ctttatgatc tgttttaatc ttgaaacgca gtttatactt tttcttagct tcaataggaa
    23281 ccatacggtg aaccgctgcc cttaatttct cagggcttga gatagtgata gccccatcct
    23341 tagcctcaat gactcgagtt gaggcatctg cagaactatt cttctggtct acccaagctg
    23401 accacccctg agctttgctt cctgtccacg gactgcttcc gtcctccgtc tgaccaaaat
    23461 caccattttc aactaaattt tttgtatccc tttgctgata ctgaacttcc ttctggcttt
    23521 gctgaatggt attcgtagtc gtttcttccg ctccaactgt catataatgg tttaataaaa
    23581 atcctgataa aacaagtgaa gacaaactca caatgagctt ctttgttttt gtttgcatgt
    23641 atacactcca ataaacatat tgtaaaatca ataggaacta aatctcaaat tagagattta
    23701 tagcaactaa gtaaaccggt ttaattaaat tataccattt ttaaagttat ttgtaaacgg
    23761 tttctatcat attgagggct tattcttcaa caatatcttc catccgactc gacgaaaaaa
    23821 gtaaaaactg ttacaatgta gaaaaagaaa agaggccctc atgacttcac tttatgattt
    23881 ttccgttttg aaccaaaaca accaagcaac tcccttggat agctatcgtg gcaaagttct
    23941 cttgattgtc aacactgcta ctggatgtgg tttaacgccc cagtaccagg gacttcaaga
    24001 actctatgaa cgctatcaag atcagggctt tgaaatattg gatttccctt gcaatcagtt
    24061 tatgggacaa gcacccggca gcgcagagga aatcaacgcc ttctgtagcc tacattttca
    24121 aaccaccttc ccacgttttg ccaagattaa ggtcaacggt aaggaagcag accctctcta
    24181 tgtctggtta aaagaccaga aatccggccc actaggaaaa cgagtcgaat ggaatttcgc
    24241 taagtttctc atcggtcgag atgggcaagt ctttgaacgc ttttcttcaa aaacagaccc
    24301 aaaacaaatt gaagaggcga tacaaactct actataattc acaatctcac tatgattagg
    24361 tttcctttaa cctgatgaat agtgagattt tttgattggc tttgacttaa atagaaaaac
    24421 accccatgat atgaaacatg aagtgttgta aagtctatgt tgtaggtgct tatttcacat
    24481 tttcaatgtg accagtgata acgaatacca tacagaatct tcatatacac taaacaaatg
    24541 actttctaat tatatcaatt agttttggct agtaaatatc atttccaaca aacgccctct
    24601 caattcctta tcctgatgat gcaagatatt cattaagtca tgagagtttt tcgcattgat
    24661 gaattgattt aacaatctat cttttaattc atatggaaga gaagctgtct ttagtagtct
    24721 aaaaacttca tcatttaaag atattgtttt attatcttta cattcaaatc tagctgtatc
    24781 attcttattt ggtaattcaa ttatagacac attcgttcct ttaaaatgaa ttctatgcct
    24841 tctattgctt ggaacgatac tagaatctcc ttgtaatgtt aactctacca tccccatttc
    24901 ccaatcgatt gataatcttg ttttatatct ttgaccattt tgatcttcaa gcatttcaaa
    24961 agaatgttgt ttgcctggga atacatacca atctacaact tcaggtaaat caacacccat
    25021 atctatctca gaaccaacca agggaatgat cgcaccactt tttgcaaaca caggtatagt
    25081 cgaaatgtct ctataaacac tcaacttcac accacctgta tattttttct ctgaaaagaa
    25141 gtcataccat tcaccttcag ggaaccatac atccactttt gcagattgga atgccaagtc
    25201 catcttttct acaatgggag ccaccattag ttctgttcca aaaaagtatt ggtttggaac
    25261 attatagctc tcatcattct ctgggtagaa ataatacatt gggctgatta atggggcacc
    25321 ttcctcgtgt gtctttacat tcatagtgta tagatatgga atcatctgat gcctcaaacg
    25381 aaggtatttc ttcataatct tagatgttgt ttctgaaaaa aaccaaggtt ctttactatt
    25441 aaaaggactt ctagaactat gtaatcgagt gatcggacta aaaacaccaa actgtagcca
    25501 tctagtttgc agctcttcgt cataatctcc cagcatgtgc ccaccgatat catggctcca
    25561 ccaactataa ccgatattag atgctgtcgc tgtaaaataa ggttgaaatc ttaaggaatt
    25621 ccaactaata atagtgtccc ctgaaaaacc aacagggtag cggtggctac caggacctgc
    25681 atatcttgat aaaatcaaac caccttctgc atttttacaa ctatcctgat aatgataatg
    25741 gtttaaaagc catagtggat ccaatatgcc ttgagtccct tgttgccagt caatccacca
    25801 aaaatctact ccctgctttt ctagctcata atgaacatct ttaaagtagg cttccctaaa
    25861 agagggatta aaaaaatcaa aaatggcagg ttcttctagt tctacattta accctaatcg
    25921 tttggcgact tgaggataag cttcttcata agcccgtata ccatcagcag gatggacatt
    25981 taaagagagt tttaactttc tatcatgaag ttgttgcaat aactgttctg gattaggtat
    26041 taagttttta ttccaactat atcctgtcca gccacttcca aaacgagctg gaatgtcagt
    26101 tatatgccaa tccatatcta acacaccgat agataatgga attttctctg tttcaaatct
    26161 gtctattaaa tccaagtatt catccgacgt ataaggccaa tatctactcc accaattccc
    26221 taaagcatat cttggtaaca agggtgttga accagtcaaa tggtaaaagt ctctgattgc
    26281 tcctctataa tcatgcccat aggcaaagaa atagaggtca atttgatttt ctctctcaat
    26341 ataaccagac tgttcatccc aaataaatcc ttgagaatca tccaataagg ctataccatt
    26401 tcgactaata attccatctt ctaatgggat tgctccatct gccttatcca gagtccgagc
    26461 tgttcctttt aacgtttcaa tcgattcacc aaaataccag cgactaccat atacggcaaa
    26521 atttcctttt aattctataa ataaattttc ggcgttaaat tctcctttat taaagtgcag
    26581 atgaaaatag tccgtcataa tatctagtac gtttgatgtc tcgatataat ctaacgaaac
    26641 ttggccaaaa tctctattat agataagttg tgtagttcta tcctcaaaac tgccagtttt
    26701 agagtattct aaccttacta gcttgtctgt taatacagag atgcgataaa actctccctt
    26761 aaaaattttc aatttgtttc cctcctttta tggtagcata aaaacagaac gcaccatttt
    26821 tgatgcgttt ttcattattc tgaatgcaat gttctatctg ttatatctat gacaaataat
    26881 agtcaattga aaaaatgtag tggacaaaat atcttttaac aaaccaagag tttattaaag
    26941 agttatcact tttcaacttt tctaagctta tgcagttgtg aaacaaacta cttttaaact
    27001 attaactaag ataggattga taaataattt caaactctta ctagcaatca tacaatattc
    27061 aagctcacgt gcttttttcc ttcctgctta tttcttagaa ctgaagaacc cggatcggta
    27121 tataaattat ccggatcaac atagtcataa gattcataac agttgcgctt cattaagtca
    27181 tccccagagc aagagcttca tctcgtaatt tttcaacatc actaaccgta ggtcgccatc
    27241 cttcaatcat atttgtactt aaagcatacc aaacactctt aaaaacggat cggttttcaa
    27301 aagctattcc catgattgtc atcttttctt tatctatatc taaggacata tgctacctcc
    27361 tttagataca ttataccatg tttctatgta gcttttaaaa attttatttt gtttgtcata
    27421 tctaagtttt cagcatgctt atcctatttt ataagcctca aacccaaata taaaacgcat
    27481 tctttttgct tttttactat tgtatcgtat tctacgataa catactttac tttattgttt
    27541 ttttaaaata acagcagttc cctgtttatc aactattcga actactttct attttgcttc
    27601 ataccctaca tagcgaaaaa atatgaaaaa gcagagaaga atatcttaaa aagacctctt
    27661 cactgctaat gttaacgctc attatttaaa ctatatggat tctatcatcg agtatacttt
    27721 tttacttatt agataccttg ctcttctttc accaattttt gatcatatac acggatgaat
    27781 ggaagataga ctaggaatgc tgcaaatgca catactagag caactaatac agctcgaaga
    27841 tctgctgtcc ctaagaaagc tccaatccct actggagttg gccatggaac ctgtgcgata
    27901 attggcttaa taaagtttag agaattcgct acgtaataaa tagtagcagt aaccattggt
    27961 gctaaaataa atggtatagc caaggctgga ttatagataa taggtaatcc aaaaattaat
    28021 ggttcattaa tattaaataa ggctggaact acagatgctc gtcctattgc tttaagctgt
    28081 tcagatttag aggcaaaagc aatatataaa catagtccta aagttgcacc agaaccacct
    28141 gcaattacaa acatattaga aaattcacct gcaacagcga agtgcccgcc agcagcattt
    28201 tcagccatgt tagcaagagc aattggacta acaaatgcaa aaacaatgtt cgcaccgtgg
    28261 atacctacaa tccaaagtag ttgagtcagt aaataaataa tcattaaacc aatccacgaa
    28321 ttagtcagat tggatacaaa accaaatgga attgcaatga ctttaaaaac atctgttccc
    28381 attgctacaa gaagaccgtt gataaagata acaacaaatg caacaacaaa tcccggaacc
    28441 aaagcggtaa atccacgaga aactccttct ggaacagctt caggcatttt aataacccaa
    28501 ttatgtttaa cacacatacg ataaataata acagtcacaa ttgccataat gattgcggta
    28561 aaaatccctg ttgtcccaaa acgtgcgact ccatttccca ttgcccatcc atctgcaatt
    28621 actgcacctt cttttagact tgtcacagtc ttcatcattc caccatcaaa aatgatttgc
    28681 ggtactgtca tgacaaaagc catcaaggca agcaaggcac cattaagagg attcatattg
    28741 agttcttctt cctctgcata aatttttgtc aattcatatg caagtgatag aacgaaataa
    28801 agagatagag aacccatagt cgcatagttt gcaaccatgt aaagtgatgt gaatttatca
    28861 aatgaagcag agaaaatatc tgccacaatt ggccaaaatg agaaagcttg tggcaaaata
    28921 ctgaatacca aaaacattga tcctacaata gtaaatggta cagcagccat acctgcagcc
    28981 gtgatagcac gcactacttt aaactgagca agtttgccca ttggtcccat aacatggttt
    29041 tcaagaaaac caaacaaccc gttttgttga tccataaata gacctcctta ataaaacata
    29101 ataattttta ctttctaaag actagtttca aatacaaatt atactagatc aggattataa
    29161 actaagtgag ttcttttcca attggacaaa ttgttgataa gccttatctg ttcgtttata
    29221 aattttttta attcttctaa tgtctaacaa actcagaact aaacctaata gaagaactac
    29281 aaaaacaaat aaacgtgcta cttggttatt ttcaaaaatc ggaaaaagat tcttaaacca
    29341 acttgtccaa gttaaaacaa gtaatcctat tgaaataagc atttgtattc taacaaacat
    29401 tagtgttatt cccaactttt ctttcctatt tccatagagt ttaaattgtt caacagttgc
    29461 taaaatagaa aatactatga gcataatggg gaaaataata ataggcgagg gattaataaa
    29521 ctgattcaaa agccaataaa tattcccaaa aaagaagagt gctattgaat aacgtagaag
    29581 aagatatcga ttaaaaaaag tattagttag agccatctct cgacgttgtt gttcaatctt
    29641 ttgtcgttct tttttatcca tatcatttcc tccttatata acaacacata tttagttaac
    29701 tttcttataa agagctaaca tttcctttgc tacttctaat aatgtcatag tggtcattaa
    29761 atgatcttga gcatgtacca tgataatttc aattttaatt tccactccac ttgcgtattc
    29821 ttgcaagagt ttggtttgtg catgatgcgc ttcaagaatt atctcatttg attgatttaa
    29881 tttactttct gcatcatcaa aactaccttc tctcattttt gcaaatgctt catgtatttc
    29941 tgaccttgca tttcccgaat gcaggataat ttcaaatgct gcaacctgca gttcctcttg
    30001 attcatatga acctcctatt ttatcttctc aaatatgtta ataaaatctt caaagttatt
    30061 gcaagatatt agctgatttt gcaattcatc attctctgtc agagagacta tctttttagt
    30121 cacagttgcc aaaccttcgt tcccatatat tgatggagat agaagaaata ctagctggac
    30181 atgtgaactt tgattatccc agagtaacga atctttacaa attgcaaccg aaacctttcc
    30241 ctctgtacca aagggctgaa taggatgcgg aactgcaatt ttttcagaaa aaacaactga
    30301 acttaattct tcgcgctgtt taattccata aagtaaagat tgttcaaact catttgattc
    30361 accaacagat aaactctcaa ccatcttttc aagtaaattt accttgtctg attcagtaca
    30421 tattaaaaag ttttctttac taaaatactg tctaaagccg ttgttttcaa atttgttaat
    30481 ctttgatgat tgtacataac tagaaacttg catctgatcc atagcttttc taatcatttc
    30541 catctcatca ctcttaagaa acacactaac tttaaaaact gggatttgaa aatatagatt
    30601 tgataaatca acagctgaca ctataaaatc tattccttta agtttttctt gattcaattc
    30661 ataatagcct attacatcaa caacttctac tcgcttccca aactccgttt ccaaacgatt
    30721 tcttaacatt tgggctgcac caaatcctgt tgcacaaata gcaagaatat taaacttagt
    30781 actctctttg ctacgttcca tagcagctaa aaagtgaaga cttacatatg ctacttcatc
    30841 atctgatatt gtccactcca agaacttgtc catatttgca agaatttctc tagtcataaa
    30901 gaatatatca ctataattct gtttaatttc atctaccaaa gggttattta aggtaatccg
    30961 gctttctaaa cgtacttgta gtgtcattag atgagttatc aatccttcaa ttagttggaa
    31021 atctgaagaa aagttataca tatcatctaa tcctaaattc tgaaatgttt taaataaaga
    31081 tttttttaaa acttcttcag aaatattctt ctgatttttt tgacattgtt gactcttagc
    31141 taacaaatgc aaagtaatgt agtctatttc ctgaactgga aattcctgat ttgttacttc
    31201 tcttacttta gaaagaattc tttgggcaac ctttctctct attgcatcat cagtcatctg
    31261 acagtctata ttttttattt caaatccgga ttttaaacga atcacagaca atgctatgtg
    31321 aactactaag ttctgtagta caaaatcaga tagttttagg ttggcctctt ggcattcatc
    31381 caaaacaatt ctagcaaatt cttctaatgg aacagtttga tcaaaaaagt taaattttac
    31441 atagcaatgt attgttttaa aaaattgatt ctctaggaaa taatttatga taaaacgtcg
    31501 tttatcacgt tcctcgcctg agacataaac tcctttattc gccctactct caatggacaa
    31561 attatactct gataacatca ctcgtatctt tctgaaatca tgagataatg ttgaacggct
    31621 aacgtaaagt tcatcagcta aatcatcaaa aagaactgga acttgctcaa ataataattt
    31681 atttaagata aatactaaac gatcatcacc ttttgaaacc gcagttttcg tatagtcttc
    31741 ttccagttca taagtttgtc taaactcctg gtaagcgcct tgattctcaa aaaatatttg
    31801 atacccttga ccttgttttg aaatcaaccg gactccttga ataatcattg tcttctcaat
    31861 taatttcagt acattacgga cagttctatc tgaacaggat aaatattctg ccagttcttt
    31921 gcttgtaaca aaacgttcct tattttttat taaaaattga aggatatctt tctctttaat
    31981 gtttaacaca ttcattccct cctaaaacgt atgttttcat atattgaagc atattataca
    32041 cttaaatcaa tttatatcaa actcaaaaca atttatctta acttaaatat ttattgacat
    32101 ttcatgtgtt catcaaatat tctcaagaat caaattagcc attttttcaa ttcccattgg
    32161 aataggaata taggcttgag gaggtatttg tacaactggt tttcctgctt tagaaccagc
    32221 ctcttcaaat tgcttaaagt acatttttgt ttgagggctg acaagataca aatcaaaagc
    32281 tgctgctgcg atagctttcc ctccttcagt agcactaata gcatcaacta caatatcttt
    32341 cccttttcct tttagaaact ctgttgtttt ctgtgccata agtgatgaag acattcctgc
    32401 tgcacaaata attaaagctt ttgccataat attttctcct tttcttaaat ccaatcaaag
    32461 ctgtgctaag ttgacttatc tgttatctat ttttattata aaataaagcg tttccaatga
    32521 caattccctc attttcctaa atgatatgga aaaaaattat ttatacttca atttataaaa
    32581 taaaattatt cctgagagta gaaatgaaac actatttgct aaaatcaaag gcaagtctcc
    32641 tatacgaata ccatgagcaa gccacaatgc aatatcaata acttgcataa catacatacc
    32701 tagagcaata gatcctgtgt cctttgtctt aactatacga aaaacttgtg gtaaaaagca
    32761 aatgttgtta aaattgctgc aatacttcca atcatatgtc atcccaatat gctaaacaaa
    32821 ctgagaataa tctcagtttg tttatactat tctactgatt caccgttaga tgaaataact
    32881 tccttatacc agccaaaaga ttttttcggg gaacgattat aacttccctt cccattgtca
    32941 tctttatcta cataaataaa accataacgt ttccgcattt caccggtacc agctgaaacc
    33001 aaatcaatac atccccatgg agtataaccc attaaatcaa caccatcttc aactacagcc
    33061 tttttcattt cacgaatatg ggcacctaga tattcaattc tataatcatc atgtaccata
    33121 ccatctgctg caacttgatc tatagctcca aaaccatttt caacaataaa gagtggtaag
    33181 tgatagtggt ctgtaaacca atttaacgca taacgtaaac cttctggatc aatttgccac
    33241 tcccattcag aagccttaac ataattattt ttcactaaat cttctgtttc aagataatca
    33301 aaataaggat tattttcacg atgagagtct atagcaaagg acatatagta actgaaacca
    33361 atgtaatcta cagtcccacc aagtaaatct tctttatcct ggtcagtaaa atcaactgaa
    33421 ataccttttc gttcccaata cttgaaaata tgctcaggat atttacctaa aacatgcaca
    33481 tcagcaaaat aataacgctt ctgcatagct ttcattgcca ttaagatatc cttaggattg
    33541 caagtagctg gataaattgg acacatcgca atcatacaac ctatttgaaa atctggatta
    33601 atctcatgac caatttttac agctcgtgca gaagcaacta gttcgtaatg tgctgcttga
    33661 tacataattg cttctctatt atcaccttcc tcatatacaa tacctgagtt agtaaatggt
    33721 gcaaaatctt cctgataatt cgcttgatta ttgatttcat tgaaagtcat ccaatattta
    33781 accttatctt tgtaacgttt aaatacgact tctgcaaaac gagcaaagaa atcaatcaat
    33841 ttcctatttt tccaaccacc atattcggtc actaagtgat aaggcatttc aaaatgagat
    33901 agagtgatga caggttcaat accattcttt aagcattcat caaaaagatt atcataaaac
    33961 tgtaatcctt cttcattcgg ctctaactca tcaccttttg gaaagatacg tgtccatgca
    34021 atagaggtac ggaagcactt gaatcccatt tcagcaaaaa gtgctatgtc ttctttataa
    34081 cgatgataaa aatctatcgc ctcatgattt ggataatatt taccctctaa aactcccgaa
    34141 gtaatttcac gagctactcc atgacgacca gcagtcataa catcagcaac actaattcct
    34201 ttgccacctt cttgccatcc gccttcaagt tgatgagcag caacagcacc accccataaa
    34261 aatctatctt taaaagtagt catctttttt cctcctgact ttgatactct tattataaac
    34321 ctcaaaccaa aaaatgaaaa cgtattcttt ttccttattg ttaaggaaag aagtaatttt
    34381 taatggaaat agaacaatat cttcttgtat tctcgtaatg atatctttac gattttcaat
    34441 actttcaaac tacaaaaact ctcacaataa ttctaattcc ctgtgtctat aaacgactta
    34501 tcgctttctg gcatcccaga atcatcttct atataacgtt caacttgcat ctgcaagtga
    34561 tatttttttc ttaaatctaa gattttctgc attgtctttg attgataatg tttatctaaa
    34621 gtttcttgat ttatccactg atcaataagg agaatagttc cctctttttc aattggtaaa
    34681 aaatattcgt atttcaagtt acctttttga tttctaattt cttcaacaag gccactatca
    34741 agcatttctc ttgcaaactt tattgcacta tctccatcac ctttataata tacatgaata
    34801 gtcaatgtca tcttatatcc tccaaaatca tccttcaatt ttaaaaaaac aagtttagat
    34861 gaggatctaa acttgttttt tatgaactaa ttatctaacg ttttgccatt actttcaatc
    34921 acttctttat accaataaaa tgattttttc ttatagcgat ttatagtcaa ttgaaacaag
    34981 aacaagacaa aagagcctca taaaaggtat ggcaacttgg taataccttt ttgaggtgct
    35041 ttttgatatg agcccat
//
LOCUS       DAVKQB010000019        34384 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKQB010000019 DAVKQB010000000
VERSION     DAVKQB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.019"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            556..1008
                     /locus_tag="VW528_000900"
     CDS             556..1008
                     /locus_tag="VW528_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9911743.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1027..2364
                     /locus_tag="VW528_000901"
     CDS             1027..2364
                     /locus_tag="VW528_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9911744.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2443..3327
                     /locus_tag="VW528_000902"
     CDS             2443..3327
                     /locus_tag="VW528_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9911745.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3327..4160
                     /locus_tag="VW528_000903"
     CDS             3327..4160
                     /locus_tag="VW528_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9911746.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4178..6271
                     /gene="nanB"
                     /locus_tag="VW528_000904"
     CDS             4178..6271
                     /gene="nanB"
                     /locus_tag="VW528_000904"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9911747.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIGEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWRYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6283..7386
                     /locus_tag="VW528_000905"
     CDS             6283..7386
                     /locus_tag="VW528_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9911748.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQLSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7559..8257
                     /locus_tag="VW528_000906"
     CDS             7559..8257
                     /locus_tag="VW528_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9911749.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8276..9793
                     /locus_tag="VW528_000907"
     CDS             8276..9793
                     /locus_tag="VW528_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9911750.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9918..11246
                     /locus_tag="VW528_000908"
     CDS             9918..11246
                     /locus_tag="VW528_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9911751.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11498..12364
                     /locus_tag="VW528_000909"
     CDS             11498..12364
                     /locus_tag="VW528_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9911752.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12380..13219
                     /locus_tag="VW528_000910"
     CDS             12380..13219
                     /locus_tag="VW528_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9911753.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13491..13943
                     /locus_tag="VW528_000911"
     CDS             13491..13943
                     /locus_tag="VW528_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9911754.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14076..14384
                     /locus_tag="VW528_000912"
     CDS             14076..14384
                     /locus_tag="VW528_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911755.1"
                     /translation="MDGQGITVDEMAWIIRGIVNGLIGRYIKLGTYAAKYGISMARSI
                     LSRAAATAAARVGLSTKISGWILRVAVNVADVYGNFANNIAAAWDAHDKIPNNGRINF
                     "
     gene            14394..14693
                     /locus_tag="VW528_000913"
     CDS             14394..14693
                     /locus_tag="VW528_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911756.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFDEHTWSTILIASLFGV
                     YDYKPIDKNRKKSKRKKKKEKIDLFMVGTYESFTDKKENSLQRKMMEEQTWHKKE"
     gene            14675..15319
                     /locus_tag="VW528_000914"
     CDS             14675..15319
                     /locus_tag="VW528_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9911757.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15488..16405
                     /locus_tag="VW528_000915"
     CDS             15488..16405
                     /locus_tag="VW528_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9911758.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16423..17307
                     /locus_tag="VW528_000916"
     CDS             16423..17307
                     /locus_tag="VW528_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9911759.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17323..18174)
                     /locus_tag="VW528_000917"
     CDS             complement(17323..18174)
                     /locus_tag="VW528_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9911760.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18374..20431
                     /gene="pbp2b"
                     /locus_tag="VW528_000918"
     CDS             18374..20431
                     /gene="pbp2b"
                     /locus_tag="VW528_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9911761.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20442..21038
                     /gene="recR"
                     /locus_tag="VW528_000919"
     CDS             20442..21038
                     /gene="recR"
                     /locus_tag="VW528_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9911762.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21214..22257
                     /locus_tag="VW528_000920"
     CDS             21214..22257
                     /locus_tag="VW528_000920"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9911763.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22341..23714
                     /locus_tag="VW528_000921"
     CDS             22341..23714
                     /locus_tag="VW528_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9911764.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELGITDLVERKDSLTFKVNFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     RLENEAK"
     gene            23701..24312
                     /locus_tag="VW528_000922"
     CDS             23701..24312
                     /locus_tag="VW528_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9911765.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKFLDGQFQEN
                     QEIADLQFFAIDQLPALSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24403..25107
                     /locus_tag="VW528_000923"
     CDS             24403..25107
                     /locus_tag="VW528_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9911766.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25326..26699
                     /gene="ftsA"
                     /locus_tag="VW528_000924"
     CDS             25326..26699
                     /gene="ftsA"
                     /locus_tag="VW528_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9911767.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26716..27975
                     /gene="ftsZ"
                     /locus_tag="VW528_000925"
     CDS             26716..27975
                     /gene="ftsZ"
                     /locus_tag="VW528_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9911768.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARTATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27980..28651
                     /locus_tag="VW528_000926"
     CDS             27980..28651
                     /locus_tag="VW528_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9911769.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDIIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28661..29200
                     /locus_tag="VW528_000927"
     CDS             28661..29200
                     /locus_tag="VW528_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9911770.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29200..29463
                     /locus_tag="VW528_000928"
     CDS             29200..29463
                     /locus_tag="VW528_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9911771.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29460..30245
                     /locus_tag="VW528_000929"
     CDS             29460..30245
                     /locus_tag="VW528_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9911772.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30254..31042
                     /gene="divIVA"
                     /locus_tag="VW528_000930"
     CDS             30254..31042
                     /gene="divIVA"
                     /locus_tag="VW528_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9911773.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31390..34182
                     /locus_tag="VW528_000931"
     CDS             31390..34182
                     /locus_tag="VW528_000931"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9911774.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGETRKFVVAAELLTSLSEK
                     FGWVDVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEYVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPTQDAVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10577 a         6055 c         7479 g        10273 t
ORIGIN      
        1 aaactaattt agagaatgaa atatagagta tttctctctt aaaagttttt ggtgaaacga
       61 gatgtagaaa ggagatttag ccaaagagtc tattagtgct agaataatag attagaatta
      121 ttttagaaaa acgaagtgag cagcttataa attcaagtcc ccaaatagat tcatactagt
      181 atcttttgca aaaaataaag ggcgacttcc ttcatgaata tcaatttcat ctataaggaa
      241 ggtagctaat tgaactaact tatttattct gtttgtcgct agaaaaatca gacctccttg
      301 tgaagattga ggagatactg aatgaaaatc aaagaagaaa ctagcaagct agtagcagat
      361 tgcccaaaac accgctttga ggttgtagat aagactgacg tatataatcc aaggtgaagc
      421 gactgtggtt tgaagagatt ttcaaagagt ataggctaga gagtagtgtt tttatgtcct
      481 tctagtagaa aatgctagac agaagaatgg ggaacttgga taggaaaaat agattgagaa
      541 aggaggttag aagagatgat tattacaaaa attagccgtt taggaactta tgtgggagta
      601 aatccacatt ttgcaacatt aatagatttt ttagaaaaaa caggactaga aaatttaaca
      661 gaaggttcga ttgctatcga tggtaatcga ttgtttggga attgctttac ttatctagca
      721 gatggtcaag caggggcttt ctttgaaaca caccaaaaat atttggatat tcatttagtt
      781 ttggaaaacg aagaagccat ggctgttaca tcgccggaaa atgtaagcgt tacccaagaa
      841 tatgatgaag agaaagatat tgaattatac acagggaaag tggaacagtt ggttcatttg
      901 agagctggtg aatgcctcat cacttttcca gaagatttac atcaacccaa ggttcgtata
      961 aatgatgaac ctgtgaaaaa agttgtcttt aaagttgcga tttcttaatg tagaaagaga
     1021 agaacgatga aaaaaatgag aaagttttta tgtctagctg gaattgcgct agcggctgtt
     1081 gccttggtag cttgttcagg aaaaaaagaa gctacaacta gtactgaacc accaacagaa
     1141 ttatctggtg agattacaat gtggcactcc tttactcaag gaccccgttt agaaagtatt
     1201 caaaaatcag cagatgcttt catgcaaaag catccaaaaa cgaaaatcaa gattgaaaca
     1261 ttttcttgga atgacttcta tactaaatgg actacaggtt tagcaaatgg aaatgtgcca
     1321 gatatcagta cagctcttcc taaccaagta atggaaatgg tcaactcaga tgctttggtt
     1381 ccgctaaatg attctatcaa gcgtattgga caagataaat ttaacgaaac tgccttaaat
     1441 gaagcaaaaa tcggagatga ttactactct gttcctcttt attcacatgc acaagtcatg
     1501 tgggttagaa cagatttgtt aaaagaacat aatattgagg ttcctaaaac ttgggatcaa
     1561 ctctatgaag cttctaaaaa attgaaagaa gctggagttt atggcttgtc tgttccgttt
     1621 ggaacaaatg acttaatggc aacacgtttc ttgaacttct acgtacgtag tggtggagga
     1681 agcctcttaa caaaagatct taaagcagac ttgacaagcc aacttgctca agatggtatt
     1741 aaatactggg ttaaattgta taaagaaatc tcacctcaag attctttgaa ctttaatgtc
     1801 cttcaacaag ctaccttgtt ctatcaagga aaaacagcat ttgactttaa ctctggcttc
     1861 catatcggag gaattaatgc caacagtcct caattgattg attcgattga tgcttatcct
     1921 attccaaaaa tcaaagagtc tgataaagac caaggaattg aaacctcaaa cattccaatg
     1981 gttgtttgga aaaattcaaa acatccagaa gttgctaaag cattcttaga agcactttat
     2041 aatgaagaag actacgttaa attccttgat tcaactccag taggtatgtt gccaactatt
     2101 aaggggatta gcgattctgc agcctataaa gaaaatgaaa ctcgtaagaa atttaaacat
     2161 gctgaagaag taattactga agctgttaaa aaaggtactg ctattggtta tgaaaatgga
     2221 ccaagtgtac aagctggtat gttaactaac caacacatta ttgaacaaat gttccaagat
     2281 atcattacaa atggaacaga tcctatgaaa gcagcaaaag aagcagaaaa acaattaaac
     2341 gatttatttg aggctgttca gtagatgtaa aagactagaa aataggtggg ctagtgagct
     2401 gaaaagctct agcccaatct tgtaaaagaa gggagaagga gaatggttaa agaacgtaat
     2461 ttaactcgct ggatatttgt tttgccagct atgattatcg taggattact ctttgtttat
     2521 ccgtttttct cgagtatttt ttatagcttt accaataagc atttgattat gcctaattat
     2581 aaatttgttg gtttggctaa ctataaagct gtgctgtcag atcccaactt ctttaatgcg
     2641 ttctttaatt caattaagtg gaccgttttc tcattagttg gtcaagtttt agtagggttt
     2701 gtattggctt tagctcttca cagagtacgc cacttcaaga aattatatag gaccttattg
     2761 attgttcctt gggcatttcc taccatcgtt attgccttct cttggcagtg gattctaaac
     2821 ggggtttatg gctacttacc taatctaatc gtaaaattag gtttaatgga acatacacct
     2881 gcatttttga cagatagtac atgggcattc ctatgtttgg tgtttatcaa catttggttt
     2941 ggagcaccaa tgattatggt taatgtgctt tcagctttgc aaacagtacc agaagaacaa
     3001 tttgaggctg ctaagataga tggtgcttca agttggcagg tgttcaagtt tatcgtcttt
     3061 ccacatatta aagtggttgt aggacttcta gttgttttga gaactgtatg gatctttaat
     3121 aactttgaca ttatctacct cattactggt ggtggaccag ccaatgctac aacgacactt
     3181 ccaatttttg cttacaacct gggttgggga actaaattgt tgggtcgtgc ttcagcagtt
     3241 acagtattgc tctttatctt cttggtggcg atttgcttta tctactttgc tatcatcagt
     3301 aagtgggaaa aggagggtag aaaataatga agaagaaatc cagtatttat ttagatattc
     3361 tctcacatgt acttttagtt ggtgcgacca tcgttgcaat tttcccattg gtatggatta
     3421 tcatatcttc tgtcaaaggg aaaggggaat taactcagta tccaacacga ttttggcctg
     3481 aacagtttac attagattat ttcactcatg ttatcaacga tttgcacttc attgataaca
     3541 ttcgaaacag tttaatcatt gccttggcta caaccctcat tgcgattatt atttctgcta
     3601 tggcagccta tggtattgtt cgattctttc ctaaattggg agcaatcatg tcgagactac
     3661 tcgtcattac ctacattttc ccaccaattt tgttagcaat tccctattca attgccattg
     3721 ctaaagttgg gttaacaaat agtttatttg gcttgatgat ggtttatcta tcttttagtg
     3781 ttccatatgc agtttggctc ttagttggat ttttccaaac agttccaatt ggaattgaag
     3841 aagcggctag aattgatggt gcaaataaat ttgttacgtt ttataaggtt gtgctaccga
     3901 ttgtagcacc aggtattgta gcaacagcta tttatacatt tatcaatgct tggaatgaat
     3961 tcctgtatgc cttgatttta attaacaata caggaaagat gacagtagca gtagcccttc
     4021 gttcacttaa tggttcagaa atactagact ggggagatat gatggcagcg tctgttattg
     4081 tagttcttcc atcaattatt ttcttctcta tcatccaaaa taagattgca agtggattat
     4141 cagaaggatc tgtgaagtag acgaaagaag gaaaaaaatg aataaaagag gtctttattc
     4201 aaaactagga atttctgttg taggcattag tcttttaatg ggagtcccca ctttgattca
     4261 tgcgaatgaa ttaaactatg gtcaactgtc catatctcct atttttcaag gaggttcata
     4321 tcaactgaac aataagagta tagatatcag ctctttgtta ttagataaat tgtctggaga
     4381 gagtcagaca gtagtaatga aatttaaagc agataaacca aactctcttc aagctttgtt
     4441 tggcctatct aatagtaaag caggctttaa aaataattac ttttcaattt tcatgagaga
     4501 ttctggtgag ataggtgtag aaataagaga cgcccaaaag ggaataaatt atttattttc
     4561 tagaccagct tcattatggg gaaagcataa aggacaggca gttgaaaata cactagtatt
     4621 tgtatctgat tctaaagata aaacatacac aatgtatgtt aatggaatag aagtgttctc
     4681 tgaaacagtt gatacatttt tgccaatttc aaatataaat ggtatagata aggcaacact
     4741 aggagctgtt aatcgtgaag gtaaggaaca ttacctcgca aaaggaagta ttggtgaaat
     4801 cagtctattt aacaaagcaa ttagtgatca ggaagtttca aatattccct tgtcaaatcc
     4861 atttcagtta attttccaat caggagattc tactcaagct aactatttta gaataccgac
     4921 actatataca ttaagtagtg gaagagttct atcaagtatt gatgcacgtt atggtgggac
     4981 tcatgattct aaaagtaaga ttaatattgc cacttcttat agtgatgata atgggaaaac
     5041 gtggagtgag ccaatttttg ctatgaagtt taatgactat gaggagcagt tagtttactg
     5101 gccacgagat aataaattaa agaatagtca aattagtgga agtgcttcat tcatagattc
     5161 atccattgtt gaagataaaa aatctgggaa aacgatatta ctagctgatg ttatgcctgc
     5221 gggtattgga aataataatg caaataaagc cgactcaggt tttaaagaaa taaatggtca
     5281 ttattattta aaactaaaga agaatggaga taacgatttc cgttatacag ttagagaaaa
     5341 tggtgtcgtt tatgatgaaa caactaataa acctacaaat tatactataa atgataagta
     5401 tgaagttttg gagggaggaa agtctttaac agtcgaacaa tattcggttg attttgatag
     5461 tggctcttta agagaaaggc ataatggaaa acaggttcct atgaatgttt tctacaaaga
     5521 ttcgttattt aaagtgactc ctactaatta tatagcaatg acaactagtc agaatagagg
     5581 agagagttgg gaacaattta agttgttgcc tccgttctta ggagaaaaac ataatggaac
     5641 ttacttgtgt cctggacaag gtttagcatt aaaatcaagt aacagattga tttttgcaac
     5701 atatactagt ggagaactaa cctatctcat ttcggatgat agtggtcaaa catggaagaa
     5761 atcctcagct tcaattccgt ttaaaaatgc aacagcagaa gcacaaatgg ttgaactgag
     5821 agatggtgtg attagaacat tctttagaac cactacaggt aagatagctt atatgactag
     5881 tagagattct ggagaaacat ggtcgaaagt ttcgtatatt gatggaattc aacaaacttc
     5941 atatggcaca caagtatctg caattaaata ctctcaatta attgatggaa aagaagcagt
     6001 cattttgagt acaccaaatt ctagaagtgg ccgtaaggga ggccaattag ttgtcggttt
     6061 ggtcaataaa gaagatgata gtattgattg gagataccac tatgatattg atttgccttc
     6121 gtatggttat gcctattctg cgattacaga attgccaaat catcacatag gtgtactgtt
     6181 tgaaaaatat gattcgtggt cgagaaatga attgcattta agcaatgtag ttcagtatat
     6241 agatttggaa attaatgatt taacaaaata aaggagaaaa acatggttaa atacggtgtt
     6301 gttggagcag ggtattttgg agctgaattg gctcgctaca tgcaaaagaa tgatggagca
     6361 gagattactc ttctctatga tccagataat gcagaggcga ttgcagaaga attgggagca
     6421 aaagtagcaa gttccttaga tgagttggtt tctagcgatg aagtagattg tgttatcgtc
     6481 gcaactccaa ataatcttca taaggaaccg gttattaagg ctgcacagca tggtaaaaat
     6541 gttttctgtg aaaaaccaat tgcgctttct tatcaagatt gtcgcgagat ggtagatgcg
     6601 tgtaaagaaa acaatgtaac ctttatggca ggacatatta tgaatttctt taatggtgtt
     6661 catcatgcaa aagaactcat taatcaagga gttatcggag acgttctata ttgtcataca
     6721 gctcgtaatg gttgggaaga acaacaactg tcagtatcat ggaaaaaaat tcgtgaaaaa
     6781 tcaggtggtc acttgtatca ccacatccat gaattggatt gcgttcaatt ccttatgggg
     6841 ggcatgcctg aaactgtaac catgacaggt ggaaatgtgg cccatgaagg tgaacatttc
     6901 ggtgatgaag atgatatgat ttttgtcaat atggaatttt ctaataagcg ttttgccttg
     6961 ttagaatggg gttcagctta tcgttggggt gaacattatg tcttaatcca aggaagcaaa
     7021 ggtgccatcc gcttagactt attcaactgt aaaggaactc ttaagctaga tgggcaagaa
     7081 agctatttct tgattcacga atcgcaagaa gaagatgatg atcggactcg tatctatcat
     7141 agtacagaga tggatggagc aattgcttat ggtaaaccag gtaaacgtac tccattatgg
     7201 ctatcatctg tcattgataa agaaatgcgc tatctgcatg agattatgga aggagctcca
     7261 gtatcagaag aatttgcaaa acttttgaca ggtgaagctg ccctagaagc aattgctact
     7321 gcagatgctt gtacccagtc tatgtttgaa gatcgcaaag taaaattgtc agaaattgta
     7381 aaataaattt tggtattctc ctattatagg tcgacttgct cctctgaaag tacttttaga
     7441 ggagctgttt gacttggcta gtttttgaaa ctgaaatcta ttatactaca aactattgaa
     7501 agcgttttaa ttttaaggta taataatctc atagaaataa agaaaaggag gaaagaggat
     7561 gccacagatt agcaaagaag ccttgattga gcaaatcaaa gatggaatca tcgtttcttg
     7621 tcaggctctt cctcatgaac cgctttatac agaagcggga ggggtgattc ccttgctggt
     7681 caaagcggct gagcaaggtg gagcagtcgg tatccgagca aacagtgttc gcgatatcaa
     7741 ggaaattaag gaagtcacta aacttccaat cattgggatt atcaaacgtg attatccacc
     7801 tcaggaaccc ttcatcacgg ctactatgaa agaagttgat gaattggcag aactggacat
     7861 cgaggtgatt gctctggatt gtaccaagcg tgaacgctac gatggtttgg aaattcaaga
     7921 gttcattcgt caggttaagg agaaatatcc taatcagctt ttgatggctg atactagtat
     7981 cttcgaagaa gggctagcag ctgtagaagc aggaattgac tttgtcggaa caaccttatc
     8041 aggctacaca tcctacagtc caaaagtaga cggtccagat tttgaattga ttaagaaact
     8101 ctgtgatgcc ggtgtagatg tcattgcaga aggaaaaatt catacaccag aacaagccaa
     8161 acaaatcctt gaatatggag tgcgaggcat cgttgttggt ggcgccatta ctagaccaaa
     8221 agagattaca gaacgcttcg ttgctagtct taaataagat gtgaggggga gttttatgtt
     8281 taaagtttta caaaaagttg gaaaagcttt tatgttacct atagctatac ttcctgcagc
     8341 aggtctactt ttggggattg gtggtgcact ttcaaaccca accacgatag caacttatcc
     8401 aatactagac aatagtattt ttcaatcaat attccaagta atgagctctg caggagaggt
     8461 tgtattcagt aatttgtcac tacttctctg tgtgggatta tgtattggct tagcgaaacg
     8521 agataaagga accgctgcgt tagcaggagt aactggttac ttagttatga ctgcaacgat
     8581 caaagctttg gtaaaacttt ttatggcaga aggatctgca attgatactg gagttattgg
     8641 agcattagtt gtcggaatag ttgccgtata tttgcacaac cgatataaca atattcaatt
     8701 accttccgct ttaggattct ttggaggttc acgcttcgtt cctattgtta catcgttctc
     8761 ttctatcttg attggctttg tcttctttgt tatttggcca cctttccaac aacttcttgt
     8821 ttctacaggt ggatatattt ctcaggcggg tccaattgga acttttctat atggattttt
     8881 aatgagactt tctggagcag taggcttaca tcatataatt taccctatgt tttggtatac
     8941 tgaacttggt ggtgttgaaa ctgttgcagg acaaacagtg gttggagctc aaaaaatatt
     9001 ttttgctcaa ttagccgatc cggcccattc tggattattt acagaaggaa caaggttttt
     9061 tgcaggtcgt ttctcaacaa tgatgttcgg tttaccggct gcctgtttag cgatgtacca
     9121 tagtgttcct aaaaatcgtc gtaaaaaata cgcgggtttg ttttttggag ttgctttaac
     9181 atcttttatt accggtatta cagaaccaat tgaatttatg tttctattcg tcagtccggt
     9241 tctatatgtt gttcacgcat tccttgatgg tgttagcttc tttattgcag acgtcttaaa
     9301 tatttcaata ggaaacacat tttcaggagg tgtaatcgat ttcactttat ttggaatttt
     9361 gcaggggaac gctaagacga attgggttct tcagattcca attggactta tttggagtgt
     9421 tttgtattat attattttta gatggttcat tactcaattc aacgttctaa cgccaggacg
     9481 aggagaagaa gtagattcta aagaaatttc tgaatccgca gattcaactt caaatactgc
     9541 agattattta aaacaggata gcctacaaat tatcagagcc ttgggtggat caaataatat
     9601 agaagatgta gatgcttgtg tgacacgttt acgtgtagct gtaaaagaag ttaatcaagt
     9661 tgataaagca cttttaaaac aaattggtgc agttgatgtc ttagaagtga agggtggcat
     9721 tcaagcaatc tatggagcaa aagcaatctt atataaaaat agtattaatg aaattttagg
     9781 tgtagatgat taagtactta ctgacttaat aaaaaacaga ggagagtgat ggatgagtag
     9841 gatgaaatga aatcgcatac aagaaataaa gaactcatta tccaagttgg atacgcttat
     9901 tacataggag aatacaaatg aaatttagaa aattagcttg tacagtactt gcgggtgctg
     9961 cggttcttgg tcttgctgct tgtggcaatt ctggcggaag taaagatgct gccaaatcag
    10021 gtggtgacgg tgccaaaaca gaaatcactt ggtgggcatt cccagtattt acccaagaaa
    10081 aaactggtga cggtgttgga acttatgaaa aatcaatcat cgaagcgttt gaaaaagcaa
    10141 acccagatat aaaagtgaaa ttggaaacca tcgacttcaa gtcaggtcct gaaaaaatca
    10201 caacagccat cgaagcagga acagctccag acgtactctt tgatgcacca ggacgtatca
    10261 tccaatacgg taaaaacggt aaattggctg agttgaatga cctcttcaca gatgaatttg
    10321 ttaaagatgt caacaatgaa aacatcgtac aagcaagtaa agctggagac aaggcttata
    10381 tgtatccgat tagttctgcc ccattctaca tggcaatgaa caagaaaatg ttagaagatg
    10441 ctggagtagc aaaccttgta aaagaaggtt ggacaactga tgattttgaa aaagtattga
    10501 aagcacttaa agacaagggt tacacaccag gttcattgtt cagttctggt caagggggag
    10561 accaaggaac acgtgccttt atctctaacc tttatagcgg ttctgtaaca gatgaaaaag
    10621 ttagcaaata tacaactgat gatcctaaat tcgtcaaagg tcttgaaaaa gcaactagct
    10681 ggattaaaga caatttgatc aataatggtt cacaatttga cggtggggca gatatccaaa
    10741 actttgccaa tggtcaaaca tcttacacaa tcctttgggc accagctcaa aatggtatcc
    10801 aagctaaact tttagaagca agtaaggtag aagtggtaga agtaccattc ccatcagacg
    10861 aaggtaagcc agctcttgaa taccttgtaa acgggtttgc agtattcaac aataaagacg
    10921 acaagaaagt cgctgcatct aagaaattca tccagtttat cgcagatgac aaggagtggg
    10981 gacctaaaga cgtagttcgt acaggtgctt tcccagtccg tacttcattt ggaaaacttt
    11041 atgaagacaa acgcatggaa acaatcagcg gctggactca atactactca ccatactaca
    11101 acactattga tggatttgct gaaatgagaa cactttggtt cccaatgttg caatctgtat
    11161 caaatggtga cgaaaaacca gcagatgctt tgaaagcctt cactgaaaaa gcgaacgaaa
    11221 caatcaaaaa agctatgaaa caatagtcct tagttattct ataaaaagta gttttttaaa
    11281 gaacctaaga gtgtataccc ccttttccct ctacacagat agtgtaagaa aagggggctt
    11341 ttgtttaaaa tgtaagaaac tgtcacgaaa ttaaaatgaa gttcttacat aagcgaatca
    11401 taaaaaattt cattttgatt ttaaaacagt tcaagaaagt caaaaaatta ttctatttga
    11461 aagagaggtg ccgactgtga aagtcaataa aatccgtatg cgggaaacag tgatttccta
    11521 cgctttccta gcaccagtat tattcttctt tgtcatcttt gtgttggctc cgatggtgat
    11581 gggcttcatt acaagtttct ttaactactc aatgactaaa tttgagtttg taggcttgga
    11641 taactatatc cgtatgttta aagatcctgt ctttacaaaa tctctgatta acacagttat
    11701 tttggttatt ggatctgtac cagttgttgt tctattctca ctctttgtag catctcagac
    11761 ctatcatcaa aatgtcattg ccagatcctt ctaccgtttc gtcttcttcc ttcctgttgt
    11821 aacgggtagt gttgccgtga cagttgtttg gaaatggatt tatgacccac tatcagggat
    11881 tctaaacttt gtccttaagt ccagccacat catcagccaa aacatttctt ggttgggaga
    11941 taaaaactgg gcattgatgg cgattatgat tattctcttg accacttcag ttggtcagcc
    12001 catcatcctt tatatcgctg ccatggggaa tattgacaat tcactggttg aagcggcgcg
    12061 tgttgatggt gcaactgagt ttcaagtttt ttggaagatt aaatggccaa gccttcttcc
    12121 aacaactctt tatattgcaa tcatcacaac aattaactca ttccagtgtt ttgccttgat
    12181 tcagcttttg acatctggtg gtccaaacta ctcaacaagt accttgatgt actaccttta
    12241 cgaaaaagcc ttccaattga cagaatacgg ctatgccaac acaattggtg tcttcttggc
    12301 agtcatgatt gctatcgtaa gctttgttca atttaaagta cttggaaacg acgtagaata
    12361 ctaaagaaag gagacagcta tgcaatctac agaaaaaaaa ccattaacag cctttactgt
    12421 tatttcaaca atcattttgc tcttgttgac tgtgctgttc atctttccat tctactggat
    12481 tttgacaggg gcattcaaat cacaacctga tacaattgtt attcctcctc agtggttccc
    12541 taaaatgcca accatggaaa acttccaaca actcatggtg cagaaccctg ccttgcaatg
    12601 gatgtggaac tcagtattta tctcattggt aaccatgttc ttagtttgtg caacctcatc
    12661 tctagcaggt tatgtattgg ctaaaaaacg tttctatggt caacgcattc tatttgctat
    12721 ctttatcgct gctatggcgc ttccaaaaca agttgtcctt gtaccattgg tacgtatcgt
    12781 caacttcatg ggaatccacg atactctctg ggcagttatc ttgcctttga ttggatggcc
    12841 attcggtgtc ttcctcatga aacaatttag tgaaaatatt cctacagagt tgcttgaatc
    12901 agctaaaatc gacggttgtg gtgagattcg taccttctgg agtgtagcct tcccgattgt
    12961 gaaaccaggg tttgcagccc ttgcaatctt taccttcatc aatacttgga atgactactt
    13021 catgcaattg gtaatgttga cttcacgtaa caatttgacc atctcacttg gggttgcgac
    13081 catgcaggct gaaatggcaa ccaactatgg tttgattatg gcaggagctg cccttgctgc
    13141 tgttccaatc gtcacagtct tcctagtctt ccaaaaatcc ttcacacagg gtattactat
    13201 gggagcggtc aaaggataat actctgagaa aatctcttca aactacgtca gcgtcacctt
    13261 gccatactta agtattgcct gcggttagct tcctagtttg ttcttcaatt ttcattgagt
    13321 ataggaaaat caatctatca agatacagaa gtatatttta tagatttaga gaatatagag
    13381 gttataagtg tctacaaaat ggagggtatg cagttacttt atgaagtttt gtcagacact
    13441 tataaactta agaatggttt tagttaacta tcagaaacga aggaaagagt atgatttttg
    13501 acgatttgaa aaacatcacc ttttacaaag ggattcatcc taatttagac aaggctatcg
    13561 actatctcta ccaacatcgt aaggattctt tcgaattagg aaagtatgat attgatggag
    13621 ataaagtctt tctagttgtt caggaaaatg tcctcaatca agctgaaaat gatcaatttg
    13681 agtatcataa gaactatgca gatttgcatt tgctggtaga aggacatgaa tattcgagct
    13741 acggttcacg tatcaaagac gaggcagtag cattcgacga agcgagtgac attggctttg
    13801 ttcattgtca tgaacactac ccactcttgt tgggttatca caattttgcg attttcttcc
    13861 caggtgagcc acatcagcca aatggttatg caggcatgga agaaaaggtt cggaaatatc
    13921 tctttaaaat tttgattgat taaaaatagg atgaattgtt tttttgtaaa gctttgacaa
    13981 tactctacca tgaaattgat ctttgtgagg tagggaaatg agaataaaat atttaaaaat
    14041 tggtatcttc taagtatgct gcaagagcta gtttcttgga tggacaggga attacagttg
    14101 atgagatggc ttggataatt aggggcattg tgaatggatt gattggtaga tacataaaat
    14161 taggtactta tgcagccaag tatggtatta gtatggcgcg ttcgatctta agtagggcag
    14221 ctgcaactgc agcagcaaga gtaggattat cgaccaagat ttctggatgg attttacgag
    14281 tagctgtgaa tgtagctgat gtatatggta attttgccaa caatattgct gcagcttggg
    14341 atgcacatga taaaattcct aacaatggtc gtataaactt ttaaaatgcg agaatgaaag
    14401 cactttgtat ttttttattg aatatgttag cttggacagt gcttgcaatg ataattcgtg
    14461 gagggctaga tggatttgat gagcatactt ggagtactat tttaattgct tcgctgttcg
    14521 gggtatatga ttataagccc atagataaaa atagaaaaaa gtccaaaaga aaaaagaaaa
    14581 aagaaaaaat agatttgttc atggtaggga cttatgaaag ctttactgac aaaaaagaaa
    14641 acagtttaca aagaaaaatg atggaggagc aaacatggca caaaaaggag taagccttat
    14701 caaggcagca tttgatacag acaactttct catgcgtttt agtgagaaag tcttggacat
    14761 cgtgacagcc aatcttcttt ttgtcgtctc ttgtttgccc atcgtgacga ttggagtggc
    14821 taaaatcagc ctctacgaga ccatgttcga agttaagaag agcagacggg tgcctgtttt
    14881 taaaatctat ctaagatctt tcaagcaaaa tctgaaacta ggtcttcagc tgggtttaat
    14941 ggagttagga attgtgtttc ttaccctttc agatctctat cttttctggg gtcaaacagc
    15001 tctgcccttc caattgctga aagccatttg tttaggtatt ctgatttttc ttactatcgt
    15061 gatgctggct agttacccta tcgcggcacg ttatgaccta tcttggaaag aaattcttca
    15121 aaaaggattg atgttggcta gttttaactt tccttggttc ttcctcatgt tagccattct
    15181 tgtcctcatt gtgatggttc tttatctgtc cgccttcagt ctactcttag gtggctcagt
    15241 cttcttactt tttgggtttg gactattggt ctttatccag actggattga tggagaaaat
    15301 tttcgcaaaa taccaatagg agccttattt ctgaaactac tttcaaacgc tccaaacgct
    15361 attctataag cgagaaacta aaatctaagt gtctataaga tattgaaagc cttttcggta
    15421 aagtgtatac taaagaagta aagaaaaatc tgctttagca gaaaataaaa ataataggag
    15481 aaaatctatg tcagatttga aaaaatacga aggtgtcatt ccagccttct acgcatgtta
    15541 tgatgaacaa ggagaagtaa gcccagaacg tacgcgtgcc ttggttcaat acttcattga
    15601 taaaggtgtt caaggtcttt atgtcaatgg ttcttctggt gaatgtatct accaaagtgt
    15661 tgaagaccgc aagttaatct tggaagaagt catggcggta gccaaaggta aattgaccat
    15721 cattgctcat gttgcctgca acaatactaa agatagtatg gaacttgctc gccatgctga
    15781 aagcttggga gtagatgcta ttgcaacgat tccaccaatt tatttccgct tgccagaata
    15841 ctcagttgcc aaatactgga acgatatcag ttctgcagct ccaaacacag actacgtgat
    15901 ttacaacatt cctcaattgg caggggttgc tttgactcca agcctttaca cagaaatgtt
    15961 gaaaaatcct cgtgttatcg gtgttaagaa ctcttctatg ccagttcaag atatccaaac
    16021 ctttgtcagc cttggtgggg aagaccatat cgtctttaat ggtcctgatg agcagttcct
    16081 aggaggacgc ctcatgggtg ctagggctgg tatcggtggt acttatggtg ctatgccaga
    16141 actcttcttg aaactcaatc agttgattgc ggataaggac ctagaaacag cgcgtgaatt
    16201 gcagtatgct atcaacgcaa tcattggtaa actcacttct gctcatggaa atatgtacgg
    16261 tgtcattaaa gaagtcttga aaatcaatga aggcttgaat attggatctg ttcgttcacc
    16321 attgacacca gtgactgaag aagatcgtcc agttgtagaa gcggctgctg ccttgattcg
    16381 tgaaaccaag gagcgcttcc tctaatctaa aaggaggtat ttatgacata ctacgttgca
    16441 attgatatcg gtggaaccaa catcaagtat ggtttggttg atcaagaggg gcaacttctt
    16501 gaatcgcatg aaatgccaac tgaggcgcat aagggtggac ctcatatctt acaaaagacc
    16561 aaagatatcg tagctagtta tttagaaaaa ggcccagtag caggtgttgc catatcttct
    16621 gctgggatgg tggatccgga taagggtgag attttctatg ctgggcctca aatccctaac
    16681 tacgcaggca cccagttcaa aaaggaaatc gaagaaagct ttactattcc ttgtgagatt
    16741 gaaaatgatg tcaactgtgc aggtcttgct gaggcagtat ctggttcagg caagggagca
    16801 agtgtgacac tttgcttgac cattggaacc ggtatcggtg gttgcttgat tatggatagg
    16861 aaagtcttcc atggttttag caattcagcc tgtgaagtcg ggtatatgca tatgcaggat
    16921 ggagcttttc aggatctcgc ttctacaaca gctttagtga aatatgtagc tgaagcccat
    16981 ggagaagatg ttgatcagtg gaatggccga cgtatcttta aggaagctac tgaaggaaac
    17041 aaaatctgca tggaaggtat tgaccgtatg gttgactatc taggaaaagg tctggcaaat
    17101 atttgttatg tggccaatcc agaagtggtt attcttggtg gtggtatcat ggggcaagag
    17161 gctatcctca aacctaagat ccgcacagcc ttgaaagagg cattggtacc aagtttagca
    17221 gaaaaaacac gattagaatt tgcccatcac caaaatacag cagggatgtt gggtgcatat
    17281 tatcatttta agacaaaaca atcctagttt ggctcagcca aactaggatt ttcttacacg
    17341 tttttgtcta cgatagccgt tgagtttttt attttcccag tagctattaa agattttttc
    17401 cttgctttcg cgattgattt ccaaaaagta ggcataaatc aaatcgataa agaagagcat
    17461 aggaagttga gcggatattc gttggatgta ggagggttgg ctgtgggtgg ctacaagaac
    17521 agtctctgta taggtctggc tatctttatt gggaacactt gtaaagagta cagtctttgc
    17581 ccccatctcc ttggcatcta atagactatc taaaatagaa ggagttgagc ctgaaagtga
    17641 gaaacccagt actagacaat tttcatccat gatgctggtt gtccaggcaa agccgtcttg
    17701 gtctgtcaaa gcttcgcaga ccacacctag tcgcataaaa cgtaatttca tttcacgggc
    17761 gacgaggcca gaactccctg ttccaaagaa gtagatacgc tcagcatctt cgattagctg
    17821 ggcaattcgc tctagttgga tttcgtcaat caagtcttgt gtttgttccc tcatattgct
    17881 ataacttctg aggactcgtt tggtcagtgg actatgcttg gagacttggt tggcttgatt
    17941 ttctgcctga tgttggtatt ggaaaataaa ttctcggtag ccagtaaagc cacacttttt
    18001 agcaaagcgg gtcaaagcag cttgagaaat atgtaatttt tgggtgactt gttgagaaga
    18061 taaatcatct gtaatcgttt cagcttgcaa aaaatagcga gcgatttctt gctctaggtc
    18121 tgtcatttct tcaaaatgtg aatcaatgat agttgcgata tctggtttgt ccatagggaa
    18181 agctccttta catgggtcat actggaagac tagatcaggg aatagtcaca cttcattata
    18241 acacataata taaggataga taaataaaaa cgcatctctg ttttaaaaac gaaaaaatcg
    18301 aaaaagcttc tctcttttcc ataattttct actcaaattg tggtacaatt aagagtaaga
    18361 ttttaagtta gaaatgagac tgatttgtat gagaaaattt aacagccatt cgattccgat
    18421 tcggcttaat ttattgtttt caatcgtcat tttactcttt atgaccatta ttggtcgttt
    18481 gttgtatatg caggttttga acaaggattt ttacgaaaaa aagctagcct cagctagtca
    18541 gaccaagatt acaagcagtt cagcccgtgg ggaaatttat gatgctagtg gaaaaccttt
    18601 ggtagaaaat acgttaaagc aggttgtttc ctttacgcgt agcaataaaa tgacggctac
    18661 agacttaaaa gaaacagcta aaaagttact gacttatgtg agcatcagtt ctccaaattt
    18721 gacagaacgc cagctggcgg attactattt ggctgatcct gaaatctata aaaaaacagt
    18781 ggaagctctc ccaagtgaga aacgcttgga ttcagatggc aatcgtctat ccgaatcaga
    18841 actgtataac aatgcggtcg atagtgtcca aacaagtcaa ctaaactata cagaggatga
    18901 aaagaaagaa atctatcttt ttagtcagtt aaatgctgtt ggaaactttg cgacaggaac
    18961 cattgctaca gatcctctaa atgattctca ggtggctgtt attgcctcta tttcaaagga
    19021 gatgcctggc attagtattt ctacttcttg ggatagaaag gttttggaaa cttccctttc
    19081 ttctatagtt gggagtgtat ccagtgaaaa agctggtctc ccagcggaag aagcagaagc
    19141 ctatcttaaa aaaggctatt ctctaaatga tcgtgtagga acctcctatt tggaaaagca
    19201 atatgaagag accttacaag gaaaacgctc ggtaaaagaa atccatctgg ataaatatgg
    19261 caatatggaa agcgtggata caattgagga aggtagtaag ggaaacaata tcaaactgac
    19321 cattgatttg gctttccaag atagcgtgga tgctttactg aaaagttatt tcaattccga
    19381 gctagaaaat ggtggagcca agtattctga aggtgtctat gcagtcgccc ttaacccaaa
    19441 aacaggtgcg gttttgtcta tgtcagggat taaacatgac ttgaaaacgg gagagttgac
    19501 gcctgattcc ttgggaacgg taaccaatgt ctttgttcca ggttcggttg tcaaggcggc
    19561 gaccatcagc tcaggttggg aaaatggagt cttgtcagga aaccagacct tgacagacca
    19621 gtccattgtc ttccaaggtt cagctcccat caattcttgg tatactcagg cttacggttc
    19681 attccctatc acagcggtcc aagctctgga gtattcatca aatacctata tggtccaaac
    19741 agccttaggt cttatggggc aaacctatca acccaatatg tttgtcggca ccagcaatct
    19801 agagtctgct atggagaaac tgcgttcaac ctttggcgaa tatggcttgg gtactgcgac
    19861 aggaattgac ctaccagatg aatctactgg atttgttccc aaagagtata gctttgctaa
    19921 ttacattact aatgcctttg ggcagtttga taactatacg ccgatgcagt tggctcagta
    19981 tgtagcaact attgcaaata atggtgttcg tgtggctcct cgtattgttg aaggcattta
    20041 tggtaataat gataagggag gactgggtga cttgattcag caactgcaac cgacagagat
    20101 gaataaggtc aatatatccg actccgatat gagcatcttg caccaaggtt tttatcaggt
    20161 tgcccatggt actagtggat tgacaactgg acgtgccttt tcaaatggcg ccttggtatc
    20221 cattagcgga aaaacaggta cagccgaaag ctatgtggca gatggtcagc aagcaaccaa
    20281 taccaatgcg gtggcctatg ccccatctga taatccccaa atcgctgttg cagtggtctt
    20341 tcctcataat accaatctaa caaatggtgt aggaccttcc attgcgcgtg acattatcaa
    20401 tctgtatcaa aaataccatc caatgaacta gaaaggaaat tatgctttat ccaacaccta
    20461 ttgccaagtt gattgacagt tattctaagt taccaggtat cgggattaag acggctacgc
    20521 gtctggcctt ttatacgatt gggatgtctg ctgatgatgt caatgaattt gcaaaaaatc
    20581 tcctttctgc taagagagaa ttgacatatt gttctatttg tggacgtttg acagacgacg
    20641 atccttgttc tatctgtact gatccgactc gtgaccagac aacaatttta gttcttgagg
    20701 atagtagaga tgtggcagcc atggaaaata tccaagaata ccatggactc tatcatgtcc
    20761 ttcatggcct catttctcct atgaatggta tcagtccgga cgatatcaat ctcaagagcc
    20821 ttatgactcg tcttatggat agtgaagttt cagaagtgat tgtggcgact aatgctacag
    20881 cggatggtga agcgacttcc atgtatcttt cacgtttgct caagccggct ggtatcaagg
    20941 ttacgcgtct agcacgaggt ctcgctgtgg gagcggacat tgagtatgcg gacgaagtga
    21001 cactcttacg agcgattgaa aatcggacag agttgtaagt gtaggcaaat ttacgaactc
    21061 cattcattta taaaaaatca aagaggctga aaatcgttcc tatcggcctc tttttgtata
    21121 gtgtgatgag taggatcagg ttcaagtttt aaaaaaccaa gcaaatatga tatactaaag
    21181 agcgagtatt ctagtagaat taggacaaat aatatgaaac aaacgattat tcttttatat
    21241 ggtggacgga gtgcggaacg cgaagtctct gtcctttcag ctgagagtgt catgcgtgcg
    21301 gtcaattacg accgtttcac agtcaagact ttctttatca gtcagtcagg tgactttatc
    21361 aaaacacagg aatttagtca tgctccgggg caagaagacc gtctcatgac caatgaaacc
    21421 attgattggg ataagaaagt tgcaccaagt gctatctacg aagaaggtgc agtggtcttt
    21481 ccagtccttc acgggccaat gggagaagat ggctctgttc aaggattctt ggaagttttg
    21541 aaaatgcctt acgttggttg caacattttg tcatcaagtc ttgccatgga taaaatcacg
    21601 actaagcgtg ttctggaatc tgctggtatt gcccaagttc cttatgtggc tatcgttgaa
    21661 ggcgatgatg tgactgctaa aatcgctgaa gtggaagaaa aattggctta tccagtcttc
    21721 actaagccgt caaacatggg gtctagtgtc ggtatttcta agtctgaaaa ccaagaagaa
    21781 ctccgtcaag ccttaaaact tgccttccga tatgacagcc gtgtcttggt tgagcaagga
    21841 gtgaatgccc gtgaaattga ggttggcctc ttgggtaact acgatgtcaa gagcacgcta
    21901 cctggagaag ttgtcaagga cgttgccttt tatgactacg atgccaagta tattgataac
    21961 aagattacta tggatattcc tgccaaaatc agtgatgatg tggtggctgt catgcgtcaa
    22021 aatgcagaaa cagccttccg tgccattggt ggccttggtc tatctcgttg cgatttcttc
    22081 tatacagata agggagagat ttttctcaac gagctcaata ccatgccagg ttttacccag
    22141 tggtctatgt acccactact ttgggacaat atggggatca gctacccaga actaatcgag
    22201 cgtttggttg accttgccaa ggaaagtttt gacaagcgcg aagcgcattt gatataaaat
    22261 gaaagagagg gtagaagcca gaaccatcac tgcacggtga ctagagttct cggacttcag
    22321 ccctttttaa aggagtagaa atgaaattaa caatccatga aattgcccaa gttgttggag
    22381 ccaaaaatga tatcagtatc tttgaggaca cccagttaga aaaagctgag tttgatagtc
    22441 gtttgattgg aactggagat ttatttgtgc cacttaaagg tgcgcgtgat ggccatgact
    22501 ttattgaaac agcctttgaa aatggtgcag cagtaacctt gtctgagaaa gaggtctcaa
    22561 atcatcctta cattctagta gatgatgttt tgacagcctt tcaatcccta gcatcctact
    22621 atcttgaaaa aacgactgtt gatgtctttg ctgttacagg ttcaaatggc aagacaacga
    22681 ctaaggatat gttggcgcat ttactgtcaa caagatacaa gacctacaaa acacaaggca
    22741 attacaataa tgagattggc cttccttaca cagttcttca tatgcctgaa ggaacagaaa
    22801 agttggtttt ggagatggga caggatcact tgggagatat tcatctcttg tctgaattgg
    22861 ctcgtccaaa aacagccatc gtgaccttgg ttggagaagc tcatttggcc tttttcaaag
    22921 atcgttctga gattgctaaa ggaaaaatgc aaattgcaga cggaatggct tcaggttcct
    22981 tgcttttagc gccggctgac cctatcgtag aggactattt gccaactgat aaaaaggtgg
    23041 ttcgttttgg tcaaggagca gagctgggaa ttaccgactt ggttgagcgc aaagatagtc
    23101 tgaccttcaa ggtcaatttc ttagagcaag cccttgattt gccagtaact ggcaagtaca
    23161 atgcgacaaa tgctatgatt gcatcctatg ttgccttgca agaaggagtt tcagaggagc
    23221 aaattcgttt ggccttccaa catcttgaat tgacgcgtaa ccgtaccgag tggaagaaag
    23281 cagccaatgg agcagatatc ctgtcagatg tttataatgc caatccaact gctatgaaac
    23341 tgattttaga gactttctct gccattccag ccaatgaagg tggcaagaaa attgcagtgt
    23401 tggcggatat gaaggagctt ggtgaccagt ctgttcaact tcataatcag atgattttga
    23461 gcctctcccc agatgtgctt gatatcgtta ttttctatgg agaagatatt gctcagttgg
    23521 ctcaattggc cagtcaaatg tttccaatcg gccacgttta ctacttcaag aaaacagaag
    23581 accaggatca atttgaagac ctagtcaagc aggttaagga aagccttgga gcctatgacc
    23641 aaatcttgct aaaaggttct aactctatga atctagccaa attggtagaa cgtttagaaa
    23701 atgaagccaa gtgattttgt caagtatctg caaagaatga ttgcccttac agatactggc
    23761 ttaaccttta caaaagatcc ttttgaccgt gagcgctacg aggacttgcg aagtctgtta
    23821 tctgaaatgt tgaatcaggg atcagacctg gatgcagaag aagtagcaga agtcttgaaa
    23881 ccaacttcag cttatgcgac tcccttaatg gacgtccgtg cttggattgt tgaggatgag
    23941 aagatttgtc tggttagggg acaaggagag gatagttggg ctttgccagg tggctttggt
    24001 gaagtcggct attctccaac tgaaaatatt ctcaaggaaa ttgaagaaga aaccggtttt
    24061 aaagccaaag ttgaaagact gctagctgtt tttgatacca atcgtttcca actacagagt
    24121 aaacaatatg caaagtttgt ttttgaatgt aagtttctcg atggacaatt ccaagaaaat
    24181 caagaaattg cagaccttca attttttgcc attgatcaac tgccagcctt atctgaaaaa
    24241 cgcattacca aggagcaaat cgagattctt tggcaggttt atcaaggtca gagggagcaa
    24301 tatcttgact aagaagatga ttatcgtatt tttaaatcca tttttaacaa ctagcatggt
    24361 ataataatat gcaggaaaat tttgaattat gaggaagact agatgaattt atgggatatt
    24421 ttctttacga ctcaggcaac cgagccgccc aaatttgacc ttttttggta tgttagccta
    24481 tttacgctct tagccttaac cttttataca gcccatcgct atcgtgaaaa gaaggtttac
    24541 caacgatttt tccaaatctt gcagactgtt cagttaatcc ttctttatgg ttggtactgg
    24601 gtcaatcata tgccactgtc agaaagccta cccttttacc attgccgtat ggctatgttt
    24661 gtggtactct tgcttcctgg tcagtccaaa tataaacaat actttgcatt attgggaaca
    24721 tttgggacat tagcagcctt tgtttatcca gtgccagatg cttacccttt tccacatatc
    24781 accattctat cctttatctt tggtcattta gcactcttgg ggaactctct agtttatcta
    24841 ttgagacagt ataatgcgcg attgctggat gtgaagggaa tttttctcat gacctttgcc
    24901 ctaaatgcct tgatttttgt ggtcaatttg gtgacaggtg gcgattacgg atttttgaca
    24961 aaaccgccat tggttgggga tcacggtcta gtagctaatt atttacttgt ttcaattgtg
    25021 ctggtagcta ctatcagttt gactaagaaa atcttagaat tctttttagc tcaagaagca
    25081 gaaaaaatga ttgcaaagga agcttaacac agagctttct tttttgctct tagagagttt
    25141 ttacaagcag cttataaaat aagaatttct gaatagacaa actcaaaaaa tggctgggaa
    25201 atttaggaaa aaagcaagca cgattaaatt ttttgtgtta taatattttg tgaatagcta
    25261 tgcctatgtt tagctatgga ataatacgaa gtgcgaaact tggaagatag agaggaagcg
    25321 atgtaatggc tagagaaggc ttttttacag gtctagatat tggaacaagc tctgtcaagg
    25381 tgcttgtggc cgagcagaga aatggtgaat taaatgtaat tggcgtgagt aatgccaaaa
    25441 gtaaaggtgt aaaggatgga attattgttg atattgatgc agcagcaact gctatcaagt
    25501 cagccatttc ccaagcggaa gaaaaggcag gcatttcgat taaatcagtg aatgtcggct
    25561 tgcctggtaa tcttttgcag gtagaaccaa ctcaggggat gattccagta acatctgata
    25621 ctaaggaaat tacggatcaa gatgttgaaa atgttgtcaa atcagctttg acaaagagta
    25681 tgacacctga ccgtgaagtc attaccttta ttcctgaaga atttattgtg gatggtttcc
    25741 aagggattcg tgacccacgt ggtatgatgg gggttcgcct tgaaatgcgt ggtttgcttt
    25801 atacaggacc tcgtactatc ttgcacaatt tgcgtaagac ggttgagcgt gcaggtgttc
    25861 aggttgaaaa tgttatcatt tcaccactag caatggttca gtctgttttg aacgaagggg
    25921 aacgtgaatt tggtgctaca gtgattgata tgggggcagg tcaaacgact gtcgctacaa
    25981 tccgtaatca agaactccag ttcacacata ttctccaaga aggtggagat tatgtaacta
    26041 aagatatctc caaggttttg aaaacctctc gcaaattagc ggaaggcttg aaactgaatt
    26101 acggggaagc ctatccgcct cttgcaagca aagaaacctt ccaagtagag gttattggag
    26161 aagtggaagc agtcgaagtg acggaagcct acttgtcaga aattatttct gcacgaatca
    26221 agcacatcct tgaacaaatc aagcaagaat tagatagaag gcgtctattg gacctccctg
    26281 gtggtattgt cttaatcggt gggaatgcca ttttaccagg tatggttgag cttgctcagg
    26341 aagtctttgg cgtccgtgtc aagctttatg ttccaaatca agttggtatc cgtaatccag
    26401 cctttgcgca tgtgattagt ttatcagaat ttgcgggtca attaacagaa gttaatcttt
    26461 tggctcaggg agcgataaaa ggtgagaatg acttaagtca tcagccaatt agttttggtg
    26521 ggatgctgca aaaaacagct cagtttgtac aatcaacgcc tgttcaacca gctcctgctc
    26581 cagaagtaga gccggtggcg cctacagaac caatggcgga tttccaacaa gcttcacaaa
    26641 ataaaccgaa attagcagat cgtttccgtg gcttgatcgg aagcatgttt gacgaataaa
    26701 gaggaaaaat aaattatgac attttcattt gatacagctg ctgctcaagg ggcagtgatt
    26761 aaagtaattg gtgtcggtgg aggtggtggc aatgccatca accgtatggt cgacgaaggt
    26821 gttacaggcg tagaatttat cgcagcaaac acagatgtac aagcattgag tagtacaaaa
    26881 gctgagactg ttattcagtt gggacctaaa ttgactcgtg gtttgggtgc aggaggtcaa
    26941 cctgaggttg gtcgtaaagc cgctgaagaa agcgaagaaa cactgacgga agctattagt
    27001 ggtgccgata tggtcttcat cactgctggt atgggaggag gctctggaac tggagctgct
    27061 cctgttattg ctcgtatcgc caaagattta ggtgcgctta cagttggtgt tgtaacacgt
    27121 ccctttggtt ttgaaggaag taagcgtgga caatttgctg tagaaggaat caatcaactt
    27181 cgtgagcatg tagacactct attgattatc tcaaacaaca atttgcttga aattgttgat
    27241 aagaaaacac cgcttttgga ggctcttagc gaagcggata acgttcttcg tcaaggtgtt
    27301 caagggatta ccgatttgat taccaatcca ggattgatta accttgactt tgccgatgtg
    27361 aaaacggtaa tggcaaacaa agggaatgct cttatgggta ttggtatcgg tagtggagaa
    27421 gaacgtgtgg tagaagcggc acgtaaggca atctattcac cacttcttga aacaactatt
    27481 gacggtgctg aggatgttat cgtcaacgtt actggtggtc ttgacttaac cttgattgag
    27541 gcagaagagg cttcacaaat tgtgaaccag gcagcaggtc aaggagtgaa catctggctc
    27601 ggtacttcaa ttgatgaaag tatgcgtgat gaaattcgtg taacagttgt cgcaacgggt
    27661 gttcgtcaag accgcgtaga aaaggttgtg gctccacaag ctagaactgc tactaactac
    27721 cgtgagacag tgaaaccagc tcattcacat ggctttgatc gtcattttga tatggcagaa
    27781 acagttgaat tgccaaaaca aaatccacgt cgtttggaac caactcaggc atctgctttt
    27841 ggtgattggg atcttcgccg tgaatcgatt gttcgtacaa cagattcagt cgtttctcca
    27901 gtcgagcgct ttgaagcccc aatttcacaa gatgaagatg aattggatac acctccattt
    27961 ttcaaaaatc gttaagtaaa tgaatgtaaa agaaaataca gaacttgttt ttcgagaagt
    28021 tgcagaggct agtctgagtg ctcatcgaga gagtggttcg gtctctgtca ttgcagttac
    28081 caagtatgta gatgtaccga cagcggaagc cttgcttccg ctaggtgttc atcatatcgg
    28141 tgaaaatcgt gtagataagt ttctggaaaa atatgaagct ttaaaagatc gagatgtgac
    28201 ttggcatttg attggtacct tgcaaagacg taaggtgaaa gatatcattc aatacgttga
    28261 ttatttccat gcattggact cagtaaagct agcaggggaa attcaaaaaa gaagtgaccg
    28321 agtcatcaag tgtttccttc aagtaaatat ttctaaagaa gaaagcaaac acggtttttc
    28381 gagagaggaa ctgctggaaa tcttgccaga gttagccaga ctagataaga ttgaatatgt
    28441 tggtttaatg acgatggcac cttttgaggc tagcagtgag cagttgaaag agattttcaa
    28501 ggcgacccaa gatttacaaa gagaaattca agagaaacaa attccaaata tgcctatgac
    28561 cgagttaagt atgggaatga gtcgtgatta taaagaagcg attcaattcg gttccacttt
    28621 tgttcgtata ggtacatcat tttttaagta ggagagaacc atgtctttaa aagatagatt
    28681 cgatagattt atagattatt ttacggagga tgaggattca agtctccctt atgaaaaaag
    28741 agatgagcct gtgtttactc cagtaaattc ttcacaggaa ccggctctcc caatgaatca
    28801 accttcacag tcggctggta caaaagagaa caatatcacc agacttcatg caagacaaca
    28861 ggaattggca aatcagagtc agcgtgcaac ggataaggtc attatagatg ttcgttatcc
    28921 tagaaaatat gaggatgcaa cagaaattgt tgatttattg gcaggaaacg aaagtatctt
    28981 gattgatttt cagtatatga cagaggtgca ggctcgtcgt tgtttggact atttggatgg
    29041 agcttgtcat gttttagctg gaaatttgaa aaaggtagct tctaccatgt atttgttgac
    29101 accagtgaat gttattgtaa atgttgaaga tatccgttta ccagatgaag atcaacaggg
    29161 tgagttcggt tttgatatga agcgaaatag agtacgataa tgattttttt aattcgtatg
    29221 atttataatg cagtggatat ttactccctg attttggtag ccttcgctgt catgtcttgg
    29281 tttccaggtg cctacgaatc cagtttaggt cgttggattg tagcgttggt gaaaccagtg
    29341 cttgctccct tgcaacgcct gcctttacag atagcgggtc ttgatttatc tgtttgggtc
    29401 gcgattgttt tggttcgatt tttaggagaa aacctagtgc gttttctggc gatgatagga
    29461 tgaataaagg gatttatcag catttctcca tagaagatcg tccatttctt gacaagggaa
    29521 tggaatggat aaagaaggta gaagatagct atgctccttt tttaactcct tttatcaatc
    29581 ctcatcagga gaagctatta aagattttgg ccaaaaccta tggtcttgct tgtagcagta
    29641 gtggggaatt cgtctcgagt gagtatgttc gagttttatt atacccagat tatttccaac
    29701 cagagttttc agattttgaa atatctctcc aggaaattgt gtattccaat aaatttgaac
    29761 atttaacgca tgctaagatt ttagggacag tcatcaatca attagggatt gaacggaaac
    29821 tttttggaga tatcctagta gatgaagaac gggcgcagat tatgattaat cagcagtttc
    29881 ttcttctctt tcaagatgga ctaaagaaaa ttggtcgtat acctgtttcg ctggaggaac
    29941 gtcctttcac cgagaaaata gataagctag aacagtatcg agaactggat ttatctgtgt
    30001 ctagttttcg attagatgtt cttttatcaa atgttttgaa actatctagg aatcaagcaa
    30061 accagttgat tgaaaagaaa cttgtccaag taaattatca tgtggtagac aaatcagatt
    30121 acactgttca agttggagac ttgattagtg tgagaaaatt tggtcgcttg agattacttc
    30181 aagataaggg acaaacgaaa aaagagaaga aaaaaataac cgtccagtta ttattaagta
    30241 agtgaggaat agaatgccaa ttacatcatt agaaataaag gacaagactt ttggaactcg
    30301 attcagaggt tttgatccag aagaagtcga tgaattttta gatattgtgg ttcgtgatta
    30361 cgaagatctt gtgcgtgcga atcatgataa aaatttgcgt attaagagtt tagaagagcg
    30421 tttgtcttac tttgatgaaa taaaagattc attgagccag tctgtattga ttgctcagga
    30481 tacagctgag agagtgaaac aggcggcgca tgaacgttca aacaatatca ttcatcaagc
    30541 agagcaagat gcgcaacgct tgttggaaga agctaaatat aaggcaaacg agattcttcg
    30601 tcaagcaact gataatgcta agaaagtcgc tgttgaaaca gaagaattga agaacaagag
    30661 ccgtgtcttc caccaacgtc tcaaatctac aattgagagt cagttggcta ttgttgaatc
    30721 ttcagattgg gaagatattc tccgtccaac agctacttat cttcaaacca gtgatgaagc
    30781 ctttaaagaa gtggttagcg aagtacttgg agaaccgatt ccagctccaa ttgaagaaga
    30841 accaattgat atgacacgtc agttctctca agcagaaatg gaagaattac aagctcgtat
    30901 tgaggtagcc gataaagaat tgtctgaatt tgaagctcag attaaacagg aagtggaaac
    30961 tccaactcct gtagtgagtc ctcaagttga agaagagcct ctgctcatcc agttggccca
    31021 atgtatgaag aaccagaagt agctccagtg catccgacag gtccaacacc agctacagaa
    31081 actgttgatt cagcaccagg atttgaagca ccgcaagaat ccgttacaat tttataagaa
    31141 acgatgtgag aacaatatct tatccttata tttccagcga gcaggagatg gtgtgagtcc
    31201 tgcattccct aacgataaga ttatcctctc aaaaactcaa gtctgaatct agtaagattt
    31261 gacgtttccc acgttacggg ataagaggga gaaagactaa atctttttcc gaataaaggt
    31321 ggtaccacga ttttcgtcct ttttggaagt cgtggttttt aatttgttat tatttataaa
    31381 ggagatacca tgaaactaaa agataccctt aatcttggga aaacagcttt cccaatgcgt
    31441 gcaggccttc cgaccaaaga gccagtttgg caaaaggaat gggaagatgc aaaactttac
    31501 caacgtcgtc aagaactaaa tgaagggaaa ccgcattttg tcttgcatga tggccctcca
    31561 tacgcaaacg gaaatatcca cgttggacat gccatgaatc atatctcaaa agatatcatc
    31621 attcgttcta agtctatgtc aggttttaac gcaccttata tcccaggttg ggatactcat
    31681 ggtctgccaa tcgagcaagt tttggcaaag caaggtgtga agcgcaaaga aatggacttg
    31741 gttgagtact tgaaactttg ccgtgaatac gctctttctc aagtttataa acaacgtgat
    31801 gatttcaagc gcttggggat gtcaggtgac tgggaaaatt tatatgtgac tttaactcca
    31861 gactacgaag cagcccaaat ccgtgtattt ggtgagatgg ctaataaggg ttatatctac
    31921 cgtggtgcta agccagttta ctggtcttgg tcatctgagt cagcccttgc cgaagcggaa
    31981 attgaatacc atgatttggt ttcaacttcc ctttactatg ccaataaggt aaaagatggc
    32041 aaaggtgttc tagatacaga tacttatatc gttgtttgga caactactcc atttactatc
    32101 acagcttctc gtggtttgac tgttggtgca gatattgatt acgttttggt tcaacctgct
    32161 ggtgaaactc gtaagtttgt cgttgctgca gaattgttga ctagcttgtc tgagaaattt
    32221 ggctgggttg atgttcaagt tttggcaact taccgtggtc aagaactcaa ccacatcgta
    32281 acagaacatc catgggatac agctgtagat gaattggtta tccttggtga ccacgttaca
    32341 actgactctg gtacaggtat tgtccataca gcccctggtt ttggtgagga cgattacaat
    32401 gttggtattg ctaataatct tgaagtcgca gtgactgttg atgaacgtgg tatcatgatg
    32461 aagaatgctg gtcctgaatt tgaaggtcaa ttctatgaaa aggtagttcc aactgttatt
    32521 gaaaaacttg gtaacctcct tcttgcccaa gaagaaatct ctcactcata tccatttgac
    32581 tggcgtacca agaaaccaat tatctggcgt gcagtaccac agtggttcgc ctctgtttca
    32641 aaattccgcc aagaaatctt ggatgctatt gataaagtta aattccatac agaatggggt
    32701 aaagtacgtc tatataatat gattcgtgac cgtggtgact gggttatctc tcgtcaacgt
    32761 gcttggggtg ttccacttcc aatcttctat gcagaagacg gtacagctat catgacagct
    32821 gagactatcg aatatgtggc tcaactcttt gaagtacatg gttcaagcat ttggtgggaa
    32881 cgtgatgcca aagacctctt gccagaagga tttacacatc caggttctcc aaatggcgag
    32941 ttcaaaaaag aaactgatat catggacgtt tggtttgact caggttcatc atggaatggt
    33001 gttttggtaa atcgtccaaa tctgacttat ccagctgacc tttaccttga aggttctgac
    33061 caataccgtg gttggttcaa ctcatcactc attacatctg ttgccaacca tggcgtagca
    33121 ccttacaaac aaatcttgtc acaaggtttt acccttgacg gtaaaggtga gaagatgtct
    33181 aaatctcttg gaaataccat tgctccaagc gatgttgaaa aacaatttgg tgctgaaatc
    33241 ttgcgtctct gggtaacaag tgttgactca agcaatgacg tgcgtatctc tatggatatc
    33301 ttgagccaag tctctgaaac ttaccgtaag attcgtaata ctcttcgttt cttgattgcc
    33361 aatacatctg actttaaccc aactcaagat gcagtcgctt acgatgaact tcgttcagtt
    33421 gataagtaca tgaccattcg ctttaaccag cttgtcaaga ccattcgtga tgcctatgca
    33481 aactttgaat tcttgacaat ctacaaggcc ttggtgaact ttatcaacgt tgatttatca
    33541 gccttttacc ttgattttgc taaagatgtt gtttacatcg aaggtgctaa atcactggaa
    33601 cgtcgtcaaa tgcagactgt cttctatgat attcttgtca aaatcaccaa actcttgaca
    33661 ccaatccttc ctcacactgc ggaagaaatc tggtcatatc ttgagtttga aacagaagac
    33721 ttcgtccaat tgtcagaatt accagaagtt caaacttttg ctaaccaaga agaaatcttg
    33781 gatacatggg cagccttcat ggactttcgt ggacaagcac aaaaagcctt ggaagaagct
    33841 cgtaatgcaa aagttatcgg taaatcactt gaagcacact tgacagttta tccaaatgaa
    33901 gtggtgaaaa ctctactcga agcagtaaat agcaatgtgg ctcaactttt gatcgtgtct
    33961 gagttgacca tcgcagaagg accagctccg gaagctgccc ttagcttcga agatgtagcc
    34021 ttcacagttg aacgtgctgc tggtgaagta tgtgaccgtt gccgtcgtat tgacccaaca
    34081 acagcagaac gtagctacca ggcagttatc tgtgaccact gtgcaagcat cgtagaagaa
    34141 aactttgcgg aagcagtcgc agaaggattt gaagagaaat aagattgaaa agtctaggca
    34201 aaattcaatt tgagaagaaa agacaactaa ttttatagtc tattaaacgc attgtatcac
    34261 gtttttgaac acctgatatg atgcgttttt tatttatttt aaaaatttgc gaggtatgac
    34321 tttttatact caacaagaat caaagagaaa cttagcaagc taacagtagt aagataaaat
    34381 agga
//
LOCUS       DAVKQB010000020        33844 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKQB010000020 DAVKQB010000000
VERSION     DAVKQB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.020"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(59..295)
                     /locus_tag="VW528_000932"
     CDS             complement(59..295)
                     /locus_tag="VW528_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9911775.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(308..781)
                     /locus_tag="VW528_000933"
     CDS             complement(308..781)
                     /locus_tag="VW528_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911776.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(799..984)
                     /gene="briC"
                     /locus_tag="VW528_000934"
     CDS             complement(799..984)
                     /gene="briC"
                     /locus_tag="VW528_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9911777.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(1143..1910)
                     /locus_tag="VW528_000935"
     CDS             complement(1143..1910)
                     /locus_tag="VW528_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9911778.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKHAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(1907..2773)
                     /locus_tag="VW528_000936"
     CDS             complement(1907..2773)
                     /locus_tag="VW528_000936"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9911779.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(2810..4177)
                     /gene="accC"
                     /locus_tag="VW528_000937"
     CDS             complement(2810..4177)
                     /gene="accC"
                     /locus_tag="VW528_000937"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9911780.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(4189..4611)
                     /gene="fabZ"
                     /locus_tag="VW528_000938"
     CDS             complement(4189..4611)
                     /gene="fabZ"
                     /locus_tag="VW528_000938"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9911781.1"
                     /translation="MIDIQEIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(4608..5093)
                     /locus_tag="VW528_000939"
     CDS             complement(4608..5093)
                     /locus_tag="VW528_000939"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9911782.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(5096..6331)
                     /gene="fabF"
                     /locus_tag="VW528_000940"
     CDS             complement(5096..6331)
                     /gene="fabF"
                     /locus_tag="VW528_000940"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9911783.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(6353..7084)
                     /gene="fabG"
                     /locus_tag="VW528_000941"
     CDS             complement(6353..7084)
                     /gene="fabG"
                     /locus_tag="VW528_000941"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9911784.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(7118..8038)
                     /gene="fabD"
                     /locus_tag="VW528_000942"
     CDS             complement(7118..8038)
                     /gene="fabD"
                     /locus_tag="VW528_000942"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9911785.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(8031..9005)
                     /gene="fabK"
                     /locus_tag="VW528_000943"
     CDS             complement(8031..9005)
                     /gene="fabK"
                     /locus_tag="VW528_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9911786.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(9124..9348)
                     /locus_tag="VW528_000944"
     CDS             complement(9124..9348)
                     /locus_tag="VW528_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9911787.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(9408..10382)
                     /locus_tag="VW528_000945"
     CDS             complement(9408..10382)
                     /locus_tag="VW528_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9911788.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(10382..10816)
                     /locus_tag="VW528_000946"
     CDS             complement(10382..10816)
                     /locus_tag="VW528_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9911789.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(10910..11695)
                     /locus_tag="VW528_000947"
     CDS             complement(10910..11695)
                     /locus_tag="VW528_000947"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9911790.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            12064..13428
                     /locus_tag="VW528_000948"
     CDS             12064..13428
                     /locus_tag="VW528_000948"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9911791.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            13431..13802
                     /locus_tag="VW528_000949"
     CDS             13431..13802
                     /locus_tag="VW528_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9911792.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            14016..15290
                     /gene="serS"
                     /locus_tag="VW528_000950"
     CDS             14016..15290
                     /gene="serS"
                     /locus_tag="VW528_000950"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9911793.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILIK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDASVTIPEALRPYMGGAEVIKP"
     gene            complement(15315..16214)
                     /locus_tag="VW528_000951"
     CDS             complement(15315..16214)
                     /locus_tag="VW528_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9911794.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(16324..16872)
                     /locus_tag="VW528_000952"
     CDS             complement(16324..16872)
                     /locus_tag="VW528_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9911795.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(17090..18412)
                     /locus_tag="VW528_000953"
     CDS             complement(17090..18412)
                     /locus_tag="VW528_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9911796.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(18503..18591)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(18755..21091)
                     /locus_tag="VW528_000954"
     CDS             complement(18755..21091)
                     /locus_tag="VW528_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9911797.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRKVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(21195..21743)
                     /locus_tag="VW528_000955"
     CDS             complement(21195..21743)
                     /locus_tag="VW528_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9911798.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQIYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(21740..22042)
                     /gene="zapA"
                     /locus_tag="VW528_000956"
     CDS             complement(21740..22042)
                     /gene="zapA"
                     /locus_tag="VW528_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9911799.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            22129..23013
                     /locus_tag="VW528_000957"
     CDS             22129..23013
                     /locus_tag="VW528_000957"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9911800.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISNPISLEE
                     KAEGKYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQG
                     LNFCAKLHFKNTEKAKNA"
     gene            23025..23639
                     /gene="lepB"
                     /locus_tag="VW528_000958"
     CDS             23025..23639
                     /gene="lepB"
                     /locus_tag="VW528_000958"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9911801.1"
                     /translation="MNLFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            23767..26133
                     /locus_tag="VW528_000959"
     CDS             23767..26133
                     /locus_tag="VW528_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9911802.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEKELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSSISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(26181..27464)
                     /locus_tag="VW528_000960"
     CDS             complement(26181..27464)
                     /locus_tag="VW528_000960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9911803.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(28308..29444)
                     /locus_tag="VW528_000961"
     CDS             complement(28308..29444)
                     /locus_tag="VW528_000961"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEU9911804.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(29506..29943)
                     /locus_tag="VW528_000962"
     CDS             complement(29506..29943)
                     /locus_tag="VW528_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9911805.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(29945..31900)
                     /locus_tag="VW528_000963"
     CDS             complement(29945..31900)
                     /locus_tag="VW528_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9911806.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(31924..33693)
                     /locus_tag="VW528_000964"
     CDS             complement(31924..33693)
                     /locus_tag="VW528_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEU9911807.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPIANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFFVA
                     ALILHADKSTEDSLEAAQEATQAAKAQSKGQLVSTSVDAVISTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
BASE COUNT        10041 a         7624 c         6384 g         9795 t
ORIGIN      
        1 caacaataaa aagaaaaagt gtttgataga tatcaaacac ttttttcttt gcctcccact
       61 atctaaaaaa atgataatag atataattgt aaacaaaaat ccagataggt tttgcatgat
      121 tgagaaagtt aaaaaaacta tggcagagaa tcgttaatct cagattgtcg gtagaacgat
      181 aaacaagggc aaaaaagaaa ccaatcagac tataatataa taaactaatt ggatctctgt
      241 gagatagtat caaatggcta atcccaaaga tgatagcaga taggataaca tccaaatagt
      301 acttggacta gggaaagaag gtattcataa aataccctct atcaagagtc tcctcaaaaa
      361 caggaccgat gattacaggc aggacaaaag ataagatagt cgataaaaag gttggttgtc
      421 catttgaaaa aagcacggta aaatactcat catgaatatt cctatgatta atcaaatgag
      481 catagcgtgc ccaaaaatta ccgagaatct gataaaccac ataagttgca aataagtaga
      541 agacaaatga ccagttccag ctctttttct caaagataaa gagcatcttt ttctttttta
      601 acctccaaat taatagaagg aaacttccca ctaatcccat tgttaaaata agagaataga
      661 catcagctcc taaccctaaa atgatcgtca catacaatcc aattgtttgt ggtaaatagg
      721 tagatagtaa aataataagc aaaaatattc caaattgtct tagttttttt gtgtttctca
      781 tcgtactttt ttgaaagatt accctgctcg gaagccgtac ttccaagcat ctatataaga
      841 attaagtgcc ccttgcctca tatagggagc aaattctcta taatataacc atctactata
      901 tccatcttcc caaacagcaa gaccacctga agtttgctcc aagtcctcag ttgaaagaac
      961 tgtaaatgta tttgtacctg tcattgcaag taccttctta aaatagattg ttgtaggctc
     1021 acatttatag tatatttctt tttttgtcta ttttatagcc catctcctca actggcaatt
     1081 tttcgacctg aattacattt ttccataaaa aatgagacct ttctagtctc atttagtcat
     1141 tcttagtatt ttctaaatcg ttgatagcgt tcttccagca actcttctag cggtttttgt
     1201 gaaagtctag ccagctccgt ttggagttct tttttgacac tcttaatcag ttctttacta
     1261 gaaagtccta tttcagaaat caccttatcc accacgtcca tttctaacag ttcatgcgaa
     1321 gtgattttca tcagttctgc tgcttccatg gcgcgagtac cgtccttcca taaaatggaa
     1381 gcaaagcctt ctggactgag aatggcatag atagaatttt ccagcatcca gacacggtcc
     1441 gcgacagcta gagccagagc cccgcctgaa ccaccttcac cgataataat ggcgataata
     1501 ggaactttca ggtcactcat ttccatgaga ttgcgagcga tagcttcccc ttgaccacgt
     1561 tcttccgctc cgacaccagg ataagcacct gctgtattga taaaggtcac aactggacgg
     1621 ccaaatttct cagcatgttt catcaaccgc agtgcctttc ggtagccttc tggatgtggt
     1681 tggccaaaat tccgtttgag gttgtcttgc aaactcttgc ctttttggat accaaccact
     1741 gttacagctt gatctccaag ccaaccaata ccaccaacaa ctgcaccatc atcacgaaaa
     1801 gaacggtcac catgtaattg gataaattca tcaaaaatgc ctgtcgcaaa gtccaaggtt
     1861 gtcaagcgac tctgctcacg cgcttctctg actatttttg caatattcat ctaggactcc
     1921 ctccatgcaa tctgactagg ctagcaatcg tatctggtaa gtctcttctt ttgacaatag
     1981 catccacaaa gccatgttct aataggaatt ctgccttttg gaaatcctca ggcaagcttt
     2041 cacgaaccgt attttcaatc acacgacgcc cagcaaaacc aaccaagctc tgtggttcag
     2101 ccagaatgat atcgccttcc atagcgaaag aagctgtcac accaccagtc gttggatctg
     2161 tcaaaatggt caggtaaaag agaccagcat ttgaatgacg tttaaccgcc gcagagatct
     2221 tagccatctg catgagactc atgattcctt cctgcatacg ggctccacca gaggctgtga
     2281 ataggacaac tggcaatttt tcgacagtcg catactcaaa caaacgagtg attttttcac
     2341 ctacaaccgt acccatagaa gccatgataa agttagaatc cataatcccg agagccacag
     2401 tctgaccttt aataagagca gttcctgtca caacggcttc atgcagacct gttttttcac
     2461 gcatagatgc cagtttcttt tggtaaccag ggaaatgcaa gggatccttg ctttcaatcc
     2521 ctgtaaacaa ttctttgaag gttcccatat caatcgtcaa agccaagcgt tcttgggcag
     2581 aaatacgaaa ggtatagcta cagtgcggac agatacgttc acttcccaga tccttctgat
     2641 agatggtatg cttacagcct ggacactggg aaaataattc atctggaacc tctggcttag
     2701 cttgaggttt ttccctaacc gaacgattgg gattgattcg aatatactta tcttttttac
     2761 taaatagagc cattgattcc ccttttcggt ttaaactctt aaagtcattt tattcttttt
     2821 cttgatattt aggtaagaag gtttccatca agaaggaagt atcataatcc ccagcaatga
     2881 cattgcgatc tgaaatgagg tcaagctgga aatctgcatt ggtctgcact ccttcaatct
     2941 ctaattcata gagggcacgt tgcattttca tcaaggcgtc aaaacgattt tcgccgtgta
     3001 ctatgatttt ggcaatcata ctatcataat aaggcggaat ggtataacct ggataaactg
     3061 ctgaatccac gcgcaagcca actccaccac ttggcagata gagattagta atcttacctg
     3121 gacttggagc aaagttaaag gctgggtttt ctgcattgat acgacactcg atggcatgac
     3181 cgcgtaggac aatatcttct tgcttaacag acaaaggctg acctgccgca atgcaaatct
     3241 gttccttaac gatatcaaca cctgaaacaa actctgttac tggatgttct acctgaacac
     3301 gagtattcat ctccatgaaa tagaaattgc tacttgcttc atcaagaaga aattcaatgg
     3361 ttcctgcatt ctcatagcca acaaactctg ccgctcgaac agcagcagca cctatttcat
     3421 gacgcagcgt ttttccgatt gcaatcgagg gactttcttc caaaaccttt tggttattcc
     3481 tttgaagaga acaatcccgc tcacccaagt gaatcacatg tccatgctca tcacctagga
     3541 tttgaacctc aatgtgccga gctggataga taacccgttc tatgtacatg gcaccattgc
     3601 cataattggc cttggcctca ctagaggcag tttcaaaggc agaaacgagg tcatctggtt
     3661 tttcaacctt acgaatccct ttaccacctc cacctgctga agccttgagc ataacaggat
     3721 agccaatttt ttcagcaaca atcaaagctt cttcagagtt atgcacttct ccatctgaac
     3781 ctggtataac aggcacacct gctttaatca tctgagcacg cgcattgatc ttatccccca
     3841 tcatatccat aacatgacca gatggaccga taaacttgat acctacttct tcacacatgg
     3901 tcgcaaattt ggaattttca ctgagaaatc caaaaccagg gtgaatagct tctgcctcag
     3961 tcaagactgc agctgataga actgcattaa tattgagata agactctgtt gccttgccag
     4021 gaccaataca aactgcttca tctgccaaaa gcgtatgaag agcttcctta tcagcagttg
     4081 aataaaccgc taccgtcgca atccccaatt cacgtgccgc acggataata cgaaccgcaa
     4141 tttcaccacg attggcaatt aaaatttttc gaaacatgga gaacctcctt agttcccaat
     4201 tgcaaaagta aggataccac tggctgcaag cttgccatcc acttcagcct ttgcttcaac
     4261 cacagctatg gtgccacgac gttttacaaa agtcgctgtc ataaccaatt ggtcgcctgg
     4321 tacaacttgc ttcttgaact taaccttgtc cataccagcg taaaagacca gttttccttt
     4381 attttcaggt tttgataact ccaacacacc agcagtttgc gccaaggctt ccataatcag
     4441 aacacctggc ataactgggt attgaggaaa gtggccgtta aagaaaggtt cgttaatggt
     4501 cacatttttg atagcaacaa tggtatcctc gctcacttcc aagacacggt ccactagaag
     4561 cataggataa cggtggggaa gagcttcttt gatttcttga atatcgatca tttgatacgt
     4621 accaatcctt taccaaactc aaccatttct tcgttagaga cgagaatttc cgttaccaca
     4681 ccatccttag gagctgggat ttcattcatg actttcatgg cttcgataat taccaatgtt
     4741 tgaccttttt tgacactatc accaactgta acgaaggcag gtttatctgg tccagcagcc
     4801 aagtaaacca ctccaacaag tggactctct acaagatttc cctcagtagc cacacttgct
     4861 tcagctggag ctggaacttc ttctgctaca gtctctgctg gagcagatgt aggagctact
     4921 ggactcggtg ttgctagaac gggtgctgga gcgacttgag ttgcaacttc aggcacaggt
     4981 ctcgcttcat tcttgctaaa ctgcaactca tccgtcccat ttttataaga aaattctctc
     5041 aaacttgact ggtcaaattg agtcatcaag tctttaatat cgtttaaatt catacttatc
     5101 tattctccca acgtttgaaa gcaagaactg cattgtggcc tccaaaacca aaagtatttg
     5161 aaatagcgta tggaatttct ttctccaagc cttgtccata aacgacatta gcttcgatat
     5221 aatctgatac ttcacttgtc ccagctgtca ttggtacaaa gttatggcgc atagcttcga
     5281 tggtgacgat agcttctact gcacccgcag cccccagcaa atgtcctgta aaagacttgg
     5341 ttgatgatac aggtacttcc ttaccaagaa cagctacgat agctccactt tctccttttt
     5401 cattggcagg agttgacgtt ccgtgagcat tgacataggc tacttgctct ggagaaatct
     5461 cagcttcttc caaggctagt ttgatggcct tgatagctcc ctgaccttct ggatgtggag
     5521 aagtcatgtg gtaggcatca caagtatttc cgtaaccaac cacttcagcc aggatagtag
     5581 ctccacgttt ttcagcgtgt tcaagacttt ctaaaaccaa catccctgaa ccttcaccca
     5641 taacaaaccc attgcgatcc ttatcaaatg ggatcgaagc acgagttgga tcctctgtag
     5701 tagagagagc tgttaaggct tggaaaccag cgatggcaaa aggtgtgata gaagcttctg
     5761 ttcctcccac caacatcaca tcttggaaac caaacttaat ggagcggaag gcatccccaa
     5821 tcgcatcatt tgatgaagag caggcagtat tgatagattt acaaacaccg tttgcaccaa
     5881 aacgcatggc tacattccca gaagccatat ttggtaaagc ttttggaaga gtcattggtt
     5941 tgacacgttt gggtcctttt tcatgaaggc gaagtacctg atcttcaatt tccttgattc
     6001 caccaatacc agatgcaacg ataacaccaa aacgatccct attaagagcc tctacatcaa
     6061 gattggcatg atttacagcc tcttgggctg catacaaggc atataaagaa tagttatcaa
     6121 aacggttggt atcttttttt acaaagtatt tatcgaacgg aaaatcttgg atttctgccg
     6181 cattatgcac atcaaagtca ctatgatcaa attttgtaat gccaccaatg ccgattttcc
     6241 cagttgctaa actattccaa aattcttctg gtgtatttcc gattggagat gttactccat
     6301 aacctgttac cactactcga tttagtttca ttcttttcac ctctagcttt cgctacatac
     6361 ttaagccacc atcaatggca atcacttgac cagttagata atcttggcct gctaaaaata
     6421 ctgtcaaatc tgcaacctgc tctgcctgcc caaattcttt catcggaatc tgagctagtg
     6481 tagcttcctt aatcttatct gataagatag ctgtcatatc agactcaatc attcctggag
     6541 caatcacatt gactcgtata ttccgactag cgacctcgcg tgccacagac ttggtaaagc
     6601 caatcaagcc agccttagaa gcagcatagt tagcttgacc aatattcccc atcaaaccaa
     6661 caacactaga catattaatg atagcacctt ctctggcttt catcatcggt ttcaagactg
     6721 attgtgtcat attaaaggca ccagtcagat tgaccttgag cactttttca aaatctgctt
     6781 ctgtcatctt gagcataaga gtatcttggg taatccctgc attgttgacc aaaacatcta
     6841 ctgaacccag ttctgcaata gcttgatcaa tcatacgctt agcgtctgca aaatctgata
     6901 catctcctga aatgggaacc accttgatac catagtttga aaactcagcg agcaattctt
     6961 ctgagattgc cccacgactg tttaagacaa tgttggctcc tgcttgagca aacttgtggg
     7021 cgatggcaag accaattcca cgactcgaac ctgtaataaa gatattttta tgttctagtt
     7081 tcattttttt cctttcaaaa cttctactta ttttagtcta tttttctaaa agtgctacta
     7141 aactcgcttg atcttccaca tgagctaagt gagcagtttg atcaattttt ttaacaaaac
     7201 ctgacaagac tttccccggt ccaatctcga taaagttgct tatgcctgct tcttgcatga
     7261 ccccaatact ttcatagaaa cgaacgggtt ccttgacctg acgcgtcaag agctgagcaa
     7321 tgtcctcttt ttgcatcaca gcagcttctg tattgccgac taggggacaa gtaaaatctg
     7381 aaaaacttac ctgagctaga gtttcagcta gtttctggct agctggctca aggagagcgg
     7441 tgtgaaaggg acctgacacc ttaagaggaa tcaagcgttt ggcacctgct tcttgcaaaa
     7501 gttcaaccgc tcgatcaact gcaaccactt ctccagcaat gacgatttgt gcaggtgtgt
     7561 tatagttggc tggagtaacc actccaagtt cagaagcttt ttgacaggct tcttcaatga
     7621 cctctactgg cgtattgaga actgctacca tcttgccaga gtcagcagga gccgcttctt
     7681 ccatataggc tccacgctta gctaccaagg caaccgcatc ttcaaaatcc aaggcgccgc
     7741 ttgccaccaa ggcagagtat tctccaagag acaaaccagc aaccatatca ggctgatagc
     7801 ccttttcttg caataaacgg tagatagcaa ccgaagtcgc tagaatggct ggttgcgtat
     7861 agcgggtctg attgagtttg tcttcttccg tatcgatgag ataacgcaaa tcataaccta
     7921 gcacctgact cgctcgatca atcgtttctt taacaatcgg atactgatca tagaaatccc
     7981 gtcccatccc tagatactgg gcaccttgac cagcaaataa aaaggctgtt ttagtcattt
     8041 cttacaactc ctgtccagcg agaggcttct tcttgaattt tcttagcggc tccgtaatac
     8101 aaatctttta ggatttcttc agctgtttct tctttagaaa caagccctgc gatttgacct
     8161 gccatgacag agccaccctc cacatcaccg tgaacaactg ctttggctag ggcaccggct
     8221 cccatttgtt caaagatttc taaatcagga tcttcctgct taaaggcatc tttttcagcc
     8281 agttcaaaat ctctagtcaa ctgattttta atagcacgaa cagcatgacc aaagtgctga
     8341 gctgaaatcg tagtgtcaat atcccttgct tttaaaattt tctccttgta gtttggatgg
     8401 gcattcgact cttttgcaac tacaaaccgt gtccccacct gtacagcctc tgcacctagc
     8461 ataaagccag ccgcagcacc ttcaccatcc gcaattcctc ctgcagcaat aacaggaata
     8521 gatacagctg tggctacctg tcgcaccaag gtcatggttg ttaatttacc gatatgcccc
     8581 ccagcttcca ttccttctgc aataacagcg tctgcaccga ttttttccat gcgtttagct
     8641 aaagcgacac taggaacgac aggaataacg attatcccag cttcatggaa acgttccata
     8701 tacttgcttg gatttcctgc tcctgttgtg acaactttaa caccttcttc aataacgaga
     8761 tccacgatat cttccacaaa gggagataag agcatgatgt tgaccccaaa gggtttatca
     8821 gtcaatgatt tgattttatc aatattggcc ttgacaactt ctttcggggc atttccccca
     8881 ccgataattc ctaatcctcc agccttggaa acagcccctg ccaaatcacc atcagcaacc
     8941 caggccatcc ctccttggaa aataggatag tcaatcttca ataattctgt aatacgcgtt
     9001 ttcatagtgc ctccaacctt ccttgcttac gtaatagttc gatttcacca taatttgaca
     9061 gtcaaactat tacctaaaca agagggagtg ggtttctccc tactccttct actaatattc
     9121 tgcttatttt gcttgctctt caacgtaagc aaccaagtca ccaactgttt tcaagtcatt
     9181 ttctgcttcg atttggatat caaaagcatc ttcgatttct gagattactt ggaacaagtc
     9241 caatgaatct gcgtccaaat catcaaaagt tgattcaagt gttacttctg atgcgtcttt
     9301 tccaagttct tcaacgataa tttcttgtac tttttcaaat actgccatga taggactcct
     9361 ttaaaataaa tagttttttt ataacaatgt gttcaccaca tgattaccta aattgtaaga
     9421 atgagcgtgc cccaggtcaa gcctccaccg aagcctgata gaagaacagt ctggctacca
     9481 tctaaaggga tgagaccttg ttctacacac tctgaaagta aaatcgggat actggctgca
     9541 ctggtattgc catattccat catattggct ggaagtttgg ctcggtcaac accaattttt
     9601 ctagccatct tatccaaaat acggtcattg gcttgatgaa gtagcagata atccaagtct
     9661 gtcacctcta taggagattc atcaatagtc tgcttgatag acttggctac atctcgaatg
     9721 gcaaaatcaa agactgtgcg tccatccatc ttcaaaaatg aatctgcact ttcttgatct
     9781 gaaaatggag aatgcaaacc tgaatgccca taagttaaac actcgctgcg acttccatcg
     9841 ctattgagac tctcagctaa gaaatgctct tgctcgctag cttctaacaa gacaccacca
     9901 gcaccatctc caaacaacac agctgttgat cgatccgacc aatcgactgc cttagagagg
     9961 gtttcactac caatcaccaa gcctttttga aagcgaccag aagcgataaa cttttcagca
    10021 gttgaaagag caaatacaaa tccactgcaa gccgcggtta agtcaaaagc aaaggcttta
    10081 ttagcaccaa tattagcttg aacacgagca gctgtagagg gcatcatcga atctggagta
    10141 atggtagcta ggatgataaa atccagttct tctcctgtta ttccagcttt tgccatcagt
    10201 ttcttagcaa cctctgtagc caaatcactg gtagattctg ttcttgaaat atgcctttgt
    10261 cgtattcccg ttcgacttga aatccactca tcattggtat ccataatctg agccaagtcg
    10321 tgatttgtaa ccacttgctc tggcacataa tgagcaacct gacttatttt tgcaaaagcc
    10381 attatttcaa atcctccaaa aattggtaaa gattagtcaa gcctttaccc atgacagcaa
    10441 tttcttcctc gctcatgcca tcaataattt tttctaccat ggccttgtgg aagcgtttat
    10501 gcagtctatg aatcaagcga cccttctttg tcaaatgcag atgcaccaca cgacgatcct
    10561 gttctgaccg aactcgctca atgtagccct tacgctctaa attgttcaaa cttgtcgtaa
    10621 cagttccaag agttaccatc aactcttttg acacttgact tggagtcaca tctggagcct
    10681 taccgatgac atcaatcgta tgcatttctt tgatggagat atccttaaaa cgactacctc
    10741 tcaagttcac ttcctcaatt acaaggacat tgttaaatat agatgttaaa tattcattga
    10801 ttcgttggta gtccattttt catatccctc cttcttcaaa aaacttttac aatcaaaaca
    10861 tttgacagaa agaattatat caaaattcaa agaatgatgc aagtattttt tattttcctg
    10921 taaatttagg tcttcttctc tccgaatgag ctcgcactcc ttctttgaaa tcctctgttt
    10981 gagctaagga tttctgtagg ttcagttcta aagtagcgta accctgccag tctttaaatt
    11041 ggctctccca aaccaacttc ttaatggcag cataggaatt acttgaagca cgtcttaatt
    11101 ttttaagaag ctgttctctc gtcttttcaa gtttttcagc ttcagaaacg cggtaaacca
    11161 acccccattc tagggctttt tctgccgtta aagcctctcc tgtcatagct aattgagcag
    11221 cacgcgtcac accgatactg cggctcaaga gatgaattcc ccctgcatct ggagccaaac
    11281 caacaccaac aaaggcttgg ataaacttag ccttatccgt cgccaaacag aaatctgccg
    11341 caacagccat attcgctgcg gcacctgcaa cagcaccgtc aacttccatt aaaacaggtt
    11401 tggctatttg cttgatttta taagaaatcg tattgaccaa ctctgcgatt tttgtcaatg
    11461 atggaatatc atcctcatcc actgcccgct tcatctctac caaatctccc ccaactgaga
    11521 agaccttccc attggcattg attaagataa aatgcacagc tggattttct tctgccaaag
    11581 tcagagcttc taaaatctcc tcacacatgg gaatatgaaa accatttgcg acctcaggac
    11641 ggttcaaggt aaggattgcc aagtcctctt caagctgata aataatgtgt tccatcaggg
    11701 ctccttttta ttttataagc agtttatact cttcgaaaat ctcttcaaac cgcgtcagct
    11761 tccatctgca acctcaaaac agtattttga gctgacttcg tcagtcttat ttacaacctc
    11821 aaagcagtgc tttgagcagt ctgcggctag cttcctagtt tgctctttga tttcattgag
    11881 tgttaaatta tttcactgtc aaagtatatc ttttttagga gcagaaagca agaaaaaatc
    11941 gactgaaagt gtctcagccg ataagaaagg ctcgctttag gctagaaagc atcttatttt
    12001 cttcattgaa aaaagccttt ctttctgcta taatcgtata aaatacttac tttaggagtt
    12061 cttatgaaag ttgttaaatt tggaggtagt tctcttgcct ctgctagtca attagaaaaa
    12121 gttttaaaca tcgtcaaaag cgattcagag cgtcgttttg tagtcgtttc tgcgcctggt
    12181 aaacgcaatg ctgaagatac taaggttacg gatgccctga ttaaatacta ccgcgactat
    12241 gttgcgggta acgatattag caagagccaa agctggatta tcgaccgcta tgctgctatg
    12301 gttagtgaat tgggactaaa accagctgtg ctagaaaaaa tttctaaaag tattcacgcc
    12361 ttggccactc ttcctattga agaaaatgaa tttctctacg atactttcct agcagccggt
    12421 gaaaataaca atgccaaatt gattgctgcc tactttaacc aaaatggtat cgatgcacgc
    12481 tatatgcacc ctagagaagc tgggattgtg gtcacaagtg aacctggtca cgctcgcatc
    12541 attccatcaa gttatgacaa gattgaagaa ttgacaaaca ccaatgaagt ccttgtcatt
    12601 cctggtttct ttggtgtcac taaggaaaat caaatctgta ctttctcacg tggaggttct
    12661 gatattacag gttctatcat tgctgctggt gtcaaagctg acctctatga aaactttacg
    12721 gacgttgatg gtatctttgc agcccaccct ggtattatcc accaaccaca ctcgattcct
    12781 gagttgacct accgtgaaat gcgcgagttg gcctatgcag gcttctcagt ccttcatgac
    12841 gaggctcttc ttcctgccta ccgtggaaaa attcctctgg ttatcaagaa taccaacaac
    12901 cctgaccatc caggtactcg tatcgttcta aaacacagta atgatgaatt tccagttgtg
    12961 ggaattgctg gtgactcagg ctttgtcagc attaacatgt cgaaatacct catgaaccgt
    13021 gaggttggat ttggccgcaa ggttctgcaa atcctggaag aacttaacat cggttgggaa
    13081 catatgccaa caggtatcga cgatctttct atcattctcc gttctcgcca actaactccc
    13141 atcaaggaag aagaaatcct gcgtcagttg gttcaaaagg ctaaagtaga tcatgcagaa
    13201 atcgaacacg acctttctat cattatgatt gttggtgaaa aaatgaagag ccacatcgga
    13261 gtgactgcta ctgcgacacg cgctctatct gaaaacaaaa tcaacatcca gatgatgtct
    13321 caaggttcta gtgaagtttc tattatgttt gttgtcaata aagaccaaga gaaagcagca
    13381 attaaagccc tctacaatgc cttttttgga gaaagtaagg aagactaagc atggcccaat
    13441 cacttaacaa aacagtactc ctcagtacga caggcacttc ctacctctct atcgctggga
    13501 aagttgggaa attccttgtc ggagatcagg ctttggaatt ttacccagat gtcaatgtcg
    13561 aacaatttat ccagattcct tggagccata tcaatcagat tggagccaat gtcactggac
    13621 gcaaaatcag ccgccacttc gaagtcttta cagacaaagg aaaattcctc tttgcttcaa
    13681 aagactcagg tgccattctc aaaattgcac gcgaaaaact aggcaatgac aaggtcgtca
    13741 aacttccgac cctgattcag acaattagcc aaaaatttaa aaatctattt gcaaaaaagt
    13801 agaaacttta gtataatcat agcaacggat aagtaggtta tcttcttctt tcagaaagtc
    13861 tgcgggtact gcgagcagat aggagggata ggtgaaattc taccgttagt aacaattaca
    13921 tacacaatca agtgcacacc tgtgaagttg gatggaaccg tggccctgcc actccaacgt
    13981 tttgtcaggc gtgctttttt cataaaggag ttcttatgtt agatatcaaa cgtattcgta
    14041 cagattttga agctgtcgca gaaaaattag ctacacgtgg tgtagatgct gctgtcttga
    14101 atgaaatgaa agaaatcgat gctaaacgtc gtaacatctt gattaaggtt gaaactctca
    14161 aagcagaacg taacacagtt tctgctgaga ttgcccaagc taagcgcaac aaggaaaata
    14221 cagatgacaa gattgctgcc atgcaaaatc tatctgctga ggttaaagcc ttggatgctg
    14281 aattggcaga aatcgatgct aaattgacag aatttacaac gactcttcca aatatcccag
    14341 ctgacagcgt tcctgttggg gctgacgaag acgacaatgt ggaagttcgc cgttggggta
    14401 ctccacgcga gtttgacttc gaacctaaag ctcactggga tctcggtgaa gaccttggta
    14461 tccttgactg ggaacgcggt ggtaaggtaa caggcgctcg cttcctcttc tataaaggcc
    14521 tcggtgctcg tttggaacgt gctatctaca actttatgtt ggatgaacat ggaaaagaag
    14581 gctatactga agtcatcaca ccttacatag tcaaccatga ttctatgttt ggtactggtc
    14641 agtatccaaa atttaaggaa gatacttttg aactcagcga taccaacttt gtcttgattc
    14701 caactgctga agttcctctg acaaactact accgtgatga aatcttagac ggcaaagatc
    14761 ttccaatcta cttcactgcc atgagtccgt cattccgttc tgaggctggt tctgccggtc
    14821 gtgatacgcg tggcttgatc cgtttgcacc aattccacaa ggttgaaatg gtcaaatttg
    14881 ccaaaccaga agaatcttac gaagaattgg aaaaaatgac agccaacgct gaaaacattc
    14941 ttcaaaaact caaccttcca taccgtgtcg ttgctctctc tactggagat atgggcttct
    15001 cagctgcgaa gacttacgac ttggaagtgt ggattccagc acaaaacaat taccgtgaaa
    15061 tctcaagctg ttcaaacaca gaagatttcc aagcccgtcg tgcccaaatc cgttaccgtg
    15121 atgaagcaga tggcaaggtg aaactccttc ataccttgaa cggttctgga cttgcagttg
    15181 gacgtacagt ggctgcaatt cttgaaaatt accaaaatga agatgcttct gtgaccatcc
    15241 cagaagcact tcgtccatac atgggtggag ctgaagtcat caaaccataa aaaataaggt
    15301 ttagctattt ctagctagac cttttttcgt aaccaaatca gataagcacc tagtacaaag
    15361 aataaaatag ttaggcatat aatggtttca gccaatacca ggtaatccag aaatggaagt
    15421 ttcaaaattc cctgagccat cttgagcgag gtcgctgtga taatggttgg gaaggtgagg
    15481 gctgagaagg ctggttgaaa accttgtttt aaaatgttgg gcagacgagt taaaacaaag
    15541 aaaaagaagg attgagaagc caaaatcatg acaatcaaga cccaagtcgg caggctggtt
    15601 cctcctactc gaactagaga agccaagagt agagagaaag gagcacagta gattccttct
    15661 tgtccaagca aggctagtgg gagtggatgt ttctttaaat cgctataaat aaggggatag
    15721 agatagaagg tcaagagaaa accaaaactc aaggtcgcat aggcaatttc gataatacct
    15781 accagaggat aggtcaaggc agccactgct atccccacat agagaaccgt ccaacttgga
    15841 gtggcatgaa ccctccgccc tggacaagca aacttgatgg taaaaccagc aatcaaggtc
    15901 aaatccaaga gaaatgaaaa ccaccaaatc ccttgtgcta ccaaaggaag ataagagaat
    15961 acgcgaaaga cataggtcga taaaatcatc ccagccatag gaaaggttgc cattcctgac
    16021 aaaagagggg gcttggtcaa ttcttgcttg gtttctttcc aattaaagag atgcagaatt
    16081 agaaagtaaa tccataaaac caaaccagtc agactaaaaa gatgggatag aaccggcaac
    16141 gtatctaaaa taagatttcc agctcctgcc aaacctagca aacaacctga aaatactaag
    16201 gggagttttt tcatcctaac ctccaataat catgttagtt tcagtataac ataaaagcgc
    16261 ttaaatgagg atttaaaaaa acgaagtccg cttatttcag acttcatttt actcagatat
    16321 gaattaggca taaggttgca attctggatt aattggtgta ttagctaagt tgttggcata
    16381 gttacagagg attgctaggc tgacaccaaa aaccacatcc aaggcatttt gttgagtgta
    16441 gccagcttct aaaaactcag acaaggcttc atctcctaca cgacccttgg tattgataac
    16501 tgccaaggta aacttagcta gggtatccaa tttaggatct gtttcaattg gagtacgatt
    16561 gcgaagagct tgaatcaagt catcattcat ctggatttgt ttgatggaaa aggctgtgtg
    16621 acctgcgaca cagaaggcac aaccattggt cacggctgcc gtgatttgca ccacttcacg
    16681 ctcaacgggt gtcaggctgt tgcgacggtg gatagatgag acaatttgat aggcttctaa
    16741 aacagtcggg gcattggcca agagaccgat taggttggga atatagccat tgttgtcttt
    16801 ttctactgtt tcaagaattt ctttcacttc tgctggtgct gactctactg tatggatagt
    16861 aaatgttgtc ataagatacc tcttttctta ttattgacac taatattatt ggaaaatctt
    16921 ataaaatcct gattcctaag tttatctaag ataaagcttt attctctcat aagattttcg
    16981 ttgttatatt agtttatcac acttccaatc acttgcataa tatatattat atatcaggct
    17041 gataaaaatt atttataggc aaaaaaatca cacgagctgt gtgattccat tatttgtcaa
    17101 aatacttttt agtttcagca ataacgactg gcgacaagac caagagggca atcaagtttg
    17161 gcagagccat caaggcgtta acgatatctg cgataatcca gaccatatcc aactcgataa
    17221 atcctcctaa caagaccatg agcacaaaaa ccacacggta gagccagata aagcgaaccc
    17281 caaagaggaa ctcaaaacag cgttctccgt aatagttcca acctagaatc gttgtaaagg
    17341 caaaaagtac aaggaagatg gtcaagaggg caggcccaaa gtgtgaaaag actgttgaga
    17401 aagctgactg agtcaaggca accccattca agtcaccact ccaaactcca gttaccaaga
    17461 tggtcaaacc agttagagta caaatgatga gggtatcaat aaaggttcct gtcatggaaa
    17521 tcaaaccttg ctctactggt tcatttgtct tggcagctgc agctgcaata ggagcagaac
    17581 ccagaccaga ttcgtttgag aacacaccac gcgccacacc attttgaata gccatccgaa
    17641 cgctagcacc agcaaatcca cctaccgcag caaggggact aaaagctgag gtaaagacta
    17701 aagcgattgt gccagggatt tttccgatat taaagaaaat aactgtaaga gttcctaaga
    17761 tataaatgat ggccataaaa ggaacaacag tagttgaaac cttagaaata gacttgagtc
    17821 caccaaagac tgcaatcgct acaaagacag acaagacgag agctgtgatg gctggcgaaa
    17881 tcgtcgttgt attttggata gattctgcaa tcgagttgac ttgggtgaag gttccgattc
    17941 ccaagagagc aaccaatact cctgctactg caaacaaaac agcaagtggt cgccactttt
    18001 ctcccatccc tagaaggata taatgcatgg gacctcccgc tactgcacca tggtcgtcct
    18061 tggtgcggta tttgatggcc aagagtcctt ccgcatactt ggtagccatt ccaaagaaag
    18121 ccgccatcca catccaaaat agagctcctg gtccaccaac cttgatagcc gtcgcaactc
    18181 ctatgatatt tcctgttcca acagttgatg ccaaggctgt acacagagct gcaaaactgg
    18241 atacatcacc atgtccctta tcctggataa aaataagctg aaaagccttg ggcagacgca
    18301 aaacctgcaa gagtcctagc cgaatagtta ggtaaatccc tgttccgacc aataaaatca
    18361 agaggggcgg tccccaagca aaagcatcga ttgatttaag caattctaac atttccttct
    18421 cctatcgttt caaccccaaa agaaagagca catgcaagat acatgtactc tggaatgctt
    18481 agataaatgc taaaaagcgg tctatcctag ctctgtcctt ttacctgaga gtttgagcag
    18541 ttgcctgcct tgccccttcg gtgcctttac ggtctctcca gagttccgtc catttacagt
    18601 catggaaaat caaacgattc cccacttcta ttaaacttca ttcggtgttg gtatttaatt
    18661 gattctaatt ttacaaaaaa tgttggcttt tgtcaatgtg tttattagta aaaattagtt
    18721 caacagtttt tactttataa agtccagaat actgctatcc tttaaaagtg acaatagtcg
    18781 caccactgcc tccagcattt tgtggggcat agccgaaact cttgacatgt ttgtttcttt
    18841 gcaagtattt ggtaactcct tcacggatga ctcctgttcc gataccatgg atgatatcaa
    18901 cttgagccat attgttaagc aaggcttggt cgatgaaggt atctagctca ttcatggctt
    18961 cttcatagcg cttgcctcga agatccagtc tagcttgagg tcctcgccca gaagttcgtt
    19021 tcacaacatt gacctgtttc ttcttgactg gtttttcttg ctgggcttga acaagatcaa
    19081 actctttctc ttccaaggtc atcttaatca agccaacttg ggcttcccag cggccgtcct
    19141 tgagttgact ggtcaaggta ccacgctgac cataactgag aaccacgata tcatctccca
    19201 cctttggagc tcgttttttc ttggcctttt gaaggacctt gtttttagac aagtccactt
    19261 tttcaggagc caattttttc aacttggcct tggcttcaat gatttcgtgg ggcttgagtt
    19321 gggatttact gtggagattt ttgagaatct ggtcactttc acttagggcc atatccacaa
    19381 tctcagcagc ctgttcacgc gccttgttaa gctcggtttc cttttcacga ttaagctcgt
    19441 tgtagagttt ttttagcgca cggttcatct tgagattttc ttgctccacc tcacggatat
    19501 tgtccaagcg tttgcggctt tccagcgtct gctcttctaa ttgctcaatg atacgattga
    19561 cgtcattgtc ctgatcgatc tgctgactgg catctcctac gataacttca gataggccta
    19621 gacgtttggc aatttcaaag gcattacttc ggccaggaac accctgcata aagcgatagg
    19681 tcgggcgaag agttgcagta tcaaactcca tactggcatt ttgcacaaag gctgtctcaa
    19741 taccgtaggc cttgagttct ggatagtggg tcgtcgccat ggtcttgatt tgacgcaggc
    19801 gaaggtcctc cagaatagcc atggcaaggg ctgctccctc ttggggatca gtaccagccc
    19861 ccaactcatc caaaagtaag agtgaatgtt ggttgacctt gccaagaata tccacgatat
    19921 tggtcatatg gctagagaag gtagacaagc tctgctcaat agactgctca tctccaatat
    19981 cagcaaaaat ttcttcaaaa ataccaacac gacttccctt gtctgctaaa atcggcaatc
    20041 ctgactgggc catgacctgt gtcaagccca gagttttgag catgatggtc ttcccacctg
    20101 tattgggacc tgtaatgaca atagctgtta aatcttgacc aaaatagaca tcatttgcga
    20161 cggcattttt gaccaaagga tggcagacat ggagcagttg aatctcttga ttttctgaca
    20221 gctgaggcac gactgcttgt ctttcttgga taaatcgaac cttggcacga atcaagtcca
    20281 gatgaccgat aatccaagca tcattagcaa tctcagccgc atgagggcgg acacgctcag
    20341 aaatttcttg gagaatgcga agcatttcat agcgctcatc tgctcgcaga ctagcaattt
    20401 cttcgctcag tttgactacc tcacggggtt cgatatagac ggtgtttcca ctagcagaaa
    20461 tatcatgaac gacacctgca atcttattgc ggtaggtgtt tttgactggt aaaaccttac
    20521 ggccatttct gctagcaaca attccttccg tcaacatctg cgctttttgc ttgagcaagt
    20581 cttgtaaaac atcgcgtacc tgactctcgc tatcatgtat ttttcgacgg attcgcgcca
    20641 attcttcact ggcaaaattt tcaatgaaac ccgcatcatt aaaggcctga agatttcctt
    20701 gtaattgcgg aaaatcatgt aatttctcaa accaaagggc taattcttcc aagctgacat
    20761 tttccagatt ggcgtaaaaa ctttgaagtt ctcggctggc aagaagcacg cgtttcaaga
    20821 gtaggaactc ctcgatattg agatccgctc ccatctccaa cctcttgcag actcctgcaa
    20881 tttccttagt tgagagaata gtaaaatgcg gttgctcgac gaaaagagcc tgcatttcct
    20941 tcatctcagc aaaagcctgt ttgattttat ctgctttggc agtcggagcc agttgtctca
    21001 attgctccaa gccctgctcg gtcaacaaat gaggctcaaa caaggccttg accttatcga
    21061 actctaatgt ttctaatatt ttcttattca tctttatttc cttgtctttg aatgtctagg
    21121 tgtggtaact ttttacattc tgatagattc aagtcaaatc tgtaaacaaa gggctaggaa
    21181 aactcctgcc ctttttatcc gattaaattt gtcacccaga tttgtttgag ccaactggtt
    21241 gttatcggta tgctctggat gatgtgtttt gcgacgatac tcttttcaag aggattttgt
    21301 ataactgcca tggggatggt cgccaagatt gtcaaggcca tttgcaagac aaataaggtc
    21361 accaacatgg acaagatacc tgctgaaact tggaacaact taccacccag ttttttgcta
    21421 ggaatcaagt gtaagagaag accaagcaaa cgaccaatgc tatagacaat cccaaataca
    21481 agcaagtagc cgatacctgc ataaaagacc ttatccagct gaaagagttg atccgatggg
    21541 aaaaagaaag tcccctgacc ttcctgcgaa tttgcataag ggagcaataa atggaattgc
    21601 tctccaagcc ccttataaaa ctggccagcc ataaaagccg atgccatggc tgaaatcagg
    21661 taataaatct gtaagagcag gcctctccga tagccgatat aaaatcccca aaccaagacc
    21721 aatagaagaa ggaatgaaat cataaggaat cctcaatctt gctctgttct tgcttacaag
    21781 tcacaagctt gtgacggagt tcttctagct cttgctcctt atcgtcaaat tcaatctcac
    21841 ggctgagctg agttgataaa cagttgactg ccaacaaaag agcgattgtt tcatcatctg
    21901 cgctaggcat ttgttcttta attgcttggt atttttctgt cgcaacctta gcgatttcct
    21961 ccataaaaag gttatcatgt tcacttgtca aggttaacga ttttttcccg aatgtaaatt
    22021 tgaatcgatt tagatttgcc ataaaattca cctcacgata ttataccaaa attcgctaat
    22081 tttgtcagtt tttacaaatt ttactgcttt tgtggtacaa tagaaactat ggcaagtatc
    22141 acactcacac caagcgaaaa ggatattcag gcttttcttg aacactatca aaccagtctg
    22201 gctcctagca agaatcccta tatccgctac tttttgaaac tacctcaagc aacggtttct
    22261 atctatactt ctggaaaaat cttgcttcag ggtgaagggg ctgaaaaata cgccagtttc
    22321 tttggctatc aagctgtaga gcaaaccagc ggacaaaatc ttcctttgat tgggacagat
    22381 gaggtaggga atggttccta ctttggtggg cttgcagttg tggcttcctt tgtcacacct
    22441 gaccagcatg ccttcttacg aaaactcggt gtgggagatt ctaagactct gaccgaccaa
    22501 aagattcgtc agattgctcc tattctcaag gaaaaaattc agcaccaggc actccttctc
    22561 tcacccagca agtacaacga ggtcatcgga gaccgctaca atgctgtttc ggttaaggtt
    22621 gccctccata atcaggctat ctatctcctc cttcaaaaag gtgttcagcc tgagaaaatt
    22681 gtgattgatg cctttaccag tgctaaaaat tatgacaagt acttggcaca agagaccaat
    22741 cgtttcagca atcctatcag caatcctatc agcttagaag aaaaggctga gggcaaatac
    22801 ttggctgtcg cagtttcttc tgtcattgcg cgtgatctct ttctggaaaa tcttgaaaat
    22861 ttgggacgag aactgggtta tcagcttcca agtggagctg gaacggcttc tgacaaggtg
    22921 gctagccaga ttttgcaagc ctatggtatg cagggactca acttctgcgc caaattgcac
    22981 tttaaaaata ctgaaaaagc gaaaaacgct tagaaaggta agttatgaat ttatttaaaa
    23041 atttcttaaa agagtgggga ttattcctcc tgattctgtc attactagct ttgagccgta
    23101 tctttttttg gagcaatgtt cgcgtagaag gacattccat ggatccgacc ctagcggatg
    23161 gtgaaatcct ctttgttgtt aagcacctcc ctattgaccg ttttgatatc gtggtggccc
    23221 atgaggaaga tggcaataag gacattgtca aacgtgtcat cggaatgcct ggcgatacca
    23281 tccgttacga aaatgataaa ctttacatca atgacaaaga aacggacgag ccttatctag
    23341 cagactatat caaacgcttc aaggatgaca aactccaaag cacttactca ggcaagggct
    23401 ttgaaggaaa taaaggaact ttctttagaa gtatcgctca aaaagcccaa gccttcacag
    23461 ttgatgtcaa ctacaacacc aactttagct ttactgttcc agaaggagaa taccttctcc
    23521 tcggagatga ccgcttggtt tcgagcgaca gccgccacgt aggtaccttc aaagcaaaag
    23581 atatcacagg ggaagctaaa ttccgcttct ggccaatcac ccgtatcgga acattttaag
    23641 agacctaaga ggccgagaat caccaatctc agcctcttct tctatcgtga gaaaatgatt
    23701 ggtaactatc taaacttacc agaacagaaa cacctcaact ctcacctatt catgcaaagg
    23761 aattttatgg aagtttattt ttcaggaacc attgaacgga ttatttttga aaatcccagc
    23821 aatttttatc gcatcctcct cctagaaatc gacgatacgg acgcagagga ttttgatgat
    23881 tttgaaatca ttgtcacagg aaccatggct gatgtaattg agggcgaaga ctatactttt
    23941 tgggggcaaa ttgtccagca ctccaagtat ggagaacaac tgcaaatcag tcgttatgat
    24001 cgcgcaaaac caactagtaa gggcttggtc aagtactttt caagtagcca tttcaagggg
    24061 attggtctca agacagctca gaaaatcgtg gatacctatg gcgaaaatac cattgacgaa
    24121 attttgcaac acccagaaaa gttagaaggc atcgcaggac tctctgccaa aaatcgcgag
    24181 gctttcgtct ccactctccg tctcaactac ggaacggaga tggttttggc caaactagcc
    24241 aactacggca ttcccaacaa actagccttt cagattcaag acttttacaa ggaagaaacc
    24301 cttgatgtgg ttgaaaatta tccctaccag ctggttgagg atatcaaggg tttgggcttt
    24361 accattgctg accaactagc tgaggaacta ggcatcgaaa gtcaggctcc tgaacgcttc
    24421 cgcgccggtc tagttcacag tctttttcag gcctgtatgg aaacagggga cacctatgtt
    24481 gaagcacggg atttgctgga acaaaccctt actctccttg agtcttcccg tcccgtggaa
    24541 ctagacccca gccaagttgc ccaagagctc tcctacctga tcgaagaaga caaggttcag
    24601 cagattgata ccaagatctt tgacaacagc ctctttttcg ctgaggaagg catccgcagt
    24661 cacttggttc gtatccttga aaaaggaaaa cagaagagtc atgatttaaa aaccattcaa
    24721 aaacatatca ctactatcga gaaagagctt ggaattgagt atgataacat tcaaaaacag
    24781 gctatctgtg atgctatcca gaataaggtc tttatcctga caggtggacc tggtactggt
    24841 aagacaactg ttatcaatgg gatcatcgct gtttacgccc tgttagaggg acttgacctc
    24901 aggaagaaaa gcaacctgcc cattcttctt gctgctccaa ctggtcgagc tgctcgtcgc
    24961 atgaatgaat tgacaggttt gcctagcgcg accatacacc gccacttggg aatgacaggt
    25021 gacgatgata ccagtcatct ggaagattac ctagatgctg actttatcat cgtggatgaa
    25081 ttctctatgg tggatacttg gctggccaat caactcttct ccagtatctc ttctaacagt
    25141 aagatcctca tcgtaggtga cagtgatcag ctaccttctg tcagtcctgg acaggttcta
    25201 gcggatctgc ttcatattcc tttgattcct cagactcgct tggaaaaaat ttatcgacaa
    25261 agcgaagaat caaccatcgt caccctagct agtcagattc gacagggcat cttgccagct
    25321 gatttcaccc aaaaaaaagc tgaccgttcc tactttgaaa ttgctagtgg ccatattcct
    25381 gccaccattg aaaaaatctt aggtgccgcc ctcagaaatg gtattcctgc ccgtgatatc
    25441 caagttctgg ctcctatgta ccgagggacg gcagggattg atgctatcaa tcagctcatg
    25501 caagacctgc tcaatccccc acaaaaagat caactcagtt ttgaagctcc tcagtgccac
    25561 tatcgtaagg gagacaaggt cattcatttg gttaacgatg ctgaaatcaa tgtctttaat
    25621 ggggatttag gagctatcac agacctgatt cctggtaaat acaccgaatc gaaacaagac
    25681 gagattgtca ttgattttga tggtaatgaa gtctcttacc cccgtaacga atggtacaag
    25741 attcgcttgg cctatgccat gagtattcat aagtctcagg gaagtgagtt cccagttgtc
    25801 atcctaccga ttactagtgc tagccgacgt atgctggagc gaaatctcat ctacacagcc
    25861 attacacgtg ccaaaagcaa actcatctta ctaggcgaat tacaagcctt cgactatgct
    25921 acccaacaca tcggaactgc ccgaaaaacc tatctgattg aacgcttcag tgatctactg
    25981 gagaatgttg aagaaaagca acaagctgtc tctgaaacag tcacatcaag tgcctctgaa
    26041 caatcctaca tcctaaccga agaaaactgg gacagcattc cagccatgat tgggattaca
    26101 gacacagacc tcaaagagat ttttggaaaa tagtacatca gaaaacccac caattcaggt
    26161 gggttttctg tttattaaga ttattttact gttgctgtgc ttgtgatcaa ttcaacagct
    26221 tttttgatag tgatatcatg tttcaacatg tcagctgaaa gcaagttttg aacttgtgca
    26281 acttccatgt tgtagtctgc tgccaattgc tcaacttctt tttggatttc ttcttctgaa
    26341 gcatcaaatc cttcagcttt ggcaactgct tcgataacaa ggttagtctt agtacgtgac
    26401 tcagcttctg cttggtattg gttgtgaagg tcttcttgag tagttccagt gatttggaag
    26461 tacatgtcag ggttgatccc ttgacgttgc aagttcccaa ggaattcatt tactgaacgg
    26521 tgaacttctt catggatcat ttcttctgga agttctacga tttcagcgtt ttctacagct
    26581 gtatcaattg ctgcaccttc aactgcatct ttgtaagctt cttctttagc agcagccaat
    26641 tctttgcggt atttttcttt caagtcagca agtgtttcaa cttcttcatc aatgtctttt
    26701 gcaagttcat cgtcaagagc tggaacttct ttagctttta cttcgtggat agttgtcacg
    26761 aatttagctt ctttacctgc aaggtcttct gcttggtagt cttctgggaa tgttacgata
    26821 acatcaacgg tttcgccagc tgagtgacct accaattggt cttcgaaacc agggatgaat
    26881 tgacctgaac caagtccaag tgagaagttt tcaccttttc caccgtcaaa ttcaacaccg
    26941 tcgatagaac caacgaagtc gatcacaaca gtgtcgccgt tttcagcagc agcttccttg
    27001 ataaccaatt cagccaggtt gttgcgttcg cgttcgatac gctcttcaac atcagcgtca
    27061 gttacttctt tttctacatc aactgatact tcaaggtttt tgtagtcacc caattttact
    27121 tcaggttttg taacgacttc agcagcgata acccagtctt gacctttttc cattgaagtt
    27181 acgtcaattt ttggttgggc aaccacttca agaccagctt cttttacagc tgcttcataa
    27241 gcgtttggca aaagagcgtt cataacgtct tggtaaagtg attcttcacc aaatttttta
    27301 tcgaagatag gacgtggaag gtgaccttta cggaaacctg gaacattaag agatttcttc
    27361 actgacttga agacacggtc caattctggt ttgatttggt cttgagagat agtgaaagtc
    27421 aagacaccac ggtttgtttc tttgttttca aatgatacag acattctgtc atttctcctt
    27481 aaaatttttt aaatacagtc tattataaca ttttctataa ataaaatcta gactaatgca
    27541 gccaattata gacaacaagc ctttttccta actaaaacac tcataaagta tagacaattt
    27601 tagactactt tggatatctt ttggttagtc attttccaaa actaatttat ctaatttttc
    27661 agttatatag tagattgaaa ctagaatagt acacctctac ttctaaaaca tttttagaaa
    27721 tcgctttgac tgtcctgatc gatttgtcct gttcttgttt cattttacta tatagatcgc
    27781 attcgaaaga attcgttcca aaattgcttg gttcgttctt ctcttggacg actaaagaat
    27841 tgatgggcat tatttttttc aacaattttc ccaccgtcca taaaaataac atgatgggct
    27901 acatctctag caaaacctat ctcataaagc tgatcaaaaa ctcaatctga ggagaaaatc
    27961 caataaggct ttccataata aaggacatcc tatcaagatt tttgaacacc cgataggctg
    28021 cctttttccg attttgaaga ctttttgtcc agcttctttt accttataaa taacaacaaa
    28081 aatcaaagac aagctagaaa gattaccgca gataactcaa acagtgtttt gaggttgcag
    28141 ataagactga cggagtcagc tcaaagcact gctttgaggt tgcagataag actgacggag
    28201 tcagctcaaa gcactgcttt gaggttgtag atagaactga cgaagtcagt aacatatgcc
    28261 taagacaaag cgatgctaac ctgatttgaa gagattttcg aaaaatatta aatatactct
    28321 acaatttgct caatcaattc ttggtcgtct aaacctgtaa cttgtataac aacatctttg
    28381 actgattgtt tagccaacaa ttcacctaat cgaatacttt cttcatcatt tacatcgcga
    28441 tagtcaaagg catagccaac tgttttaagt aggtttttat atgacaaact gagttctttc
    28501 aattcacgta tcggccggat gaatcgttca ttatagccta attttcggat tggagtacga
    28561 gctacgcgac taacctcgtc cactatgaaa gggttttcaa accgttctat aatgactttg
    28621 tgataattct ccaattcttt tttatcaaag ttccatttgg caatcaagag actccgaatt
    28681 tcagctaata cagattcaat ccgagattta atattaggat tttgaagagc ttccaaaatt
    28741 gtcttggcac cataatgcgc accaatgtaa gctgaagttg catgtccaga attgactgaa
    28801 aaaagttttc gctcaataaa gggttctaaa tcttcttcat aatgcacatc ttttagacgt
    28861 aaatctggat ttttaagacg cttggtttcc acgacccatt cattaaaggg ctcgaccaca
    28921 acaaaaaggg aatcttcgtg actttgtgct ggaacaatcc tgtctactgc agcatttgga
    28981 aaacctatgt agttatcagc aaatgtcaaa ccttccggac ttaaatattt cttgacttct
    29041 tgataaagaa attgagaccc gccaatcata ttttcacagg ccataacatc caatacctgt
    29101 gtattccctg caactcggcg agcttcgatt cctttggcta gaagttcagc gataaaaggg
    29161 agtatattag gtccgattgc agtagtaata atatccgtct tttgaatcgc ttcaatgact
    29221 tgctcaggat gttctttgct attaattcca gccacattag ttacttctat acgagactgt
    29281 cctttctgtg caatttcaat ttcatacttg cccttttcat ttagagcatg aattatctga
    29341 ttattgacat ccacaaaatc aatatggaaa ccatttttaa atagaatttc acctataaaa
    29401 ccacgaccga tattaccggc accaaaatga acagaatgtt tcattcaatt tctccttttc
    29461 ctatttgagg gacatatata ctagatggca aatgacgaaa ttcaattatt caacagcatc
    29521 aaataagcgc aatacttcct cttttgactg agcatcggct aatttaagaa cattatctac
    29581 atctgcacag aagatagaaa ttttctgaat aatttctaag tgttcattac caataccagc
    29641 aataccaaaa agaaccgttg ccacttgagg gttagataca ttcccaaaat caacccctct
    29701 agggacttga acgactgtaa taccagactt gaggacatcc ttttttgctg catctgttcc
    29761 atgcggtatt gcgataaagt tacccatgta aacagatagc tctttatctc gctcaatcat
    29821 ggcttcaata tagtcttcat taacatatcc accctcataa agaacttgcc cacaataaca
    29881 aatagcttcc tccttgttag aaaattgttt attaagctta atcaaatgtt tttcaagttt
    29941 catattagtt ctccaatttc ttaatttttt cgttaaaaat agtatttagc atctgataaa
    30001 taatggattg atttcccgtc ttgtaaatct ctgtataaag atgattttca ataatcgact
    30061 gactaatagc tgtcattaaa tctctcgctt cctcgctagc ttctttagac attagcatta
    30121 gcagacacct tttgacctct tcttcctctc gtttcattga caatgcggag ataggtagtt
    30181 tgagttcaaa catagtaaaa ctatttgttg ttactttgct agactgacta tgtaaaagca
    30241 ccagaccagt attaggaata gccataggac tattctggaa gtgagataac aattttcttg
    30301 ttaggtaatc tctgtcactc acattcttct gatattggat gatttgttct accgtgtgct
    30361 caaatgattg attattctcc aattgaacca actcgaattg actcaaaagc tgactactag
    30421 atattaaata atcttgcaag tcataacttt tctctggagc gatagcatca cgcgcgacaa
    30481 ttgtacgatg tgcctgtaca tactgaagtc gttcctgtaa ttcaagcaat tctttgacgg
    30541 ttggaaaact agaaattaca tcgatttcct gattagtcag cggcttggta gataaaatat
    30601 aatcatactg actgagatca atactgtggt aatctactag agactgaata tctatcaact
    30661 ctacaaaagg agcaacactc ttaattttac tgactaataa atctctagtg acccgacgtt
    30721 catccgttaa aagcaaaaca cggactggat aaatctctgg actacggcct aagctagaga
    30781 taaaatgtag ggcaatcatg ccatactcat actctgtatg aagatatttc ggaaaaatat
    30841 cattcactaa aagactgatg actgtataaa gaaatttatt cctttcaact aaaatctgga
    30901 cagattctgg caaattttca ccctgaaaaa ggatagggac gcctaaactg agccgaatat
    30961 gatggaaaag aaaattgaat aaaaccttgt ccttaaaaaa gtcaatcttg gtatacatgg
    31021 aaaccgtatc aatcagattt gaaatattgt agaaaaattc accatcaaat ttctccgtaa
    31081 agagcggatt gtcctgacgt ttaatgatta attcatccaa gatgctcgca aaatagataa
    31141 tttcctgaat actataaaat tgtgcagtct gcttagagta agcttgaaaa attttttgag
    31201 aaatttccaa agcctgctta ctagtattag gtgaattttc aatgttttgc tcctgaccta
    31261 taagagataa caagatcgca aaaaacatct tcatcctagc atccatatct ggaaaacctg
    31321 acagttgctt attaacaatc tgactggcca gcctagttct atctgcttct aaaatatcaa
    31381 agctcccaaa attaccggtt gaaaaatctg ctactgagat acagtttgtc agtaaaatag
    31441 ccaaaaatct tctcttacca actgaatcac cagaaatccg ataacctttt tgtcgttcaa
    31501 ttttcagatc aaagtctaaa agacgcttat caatatctga aatatcctga ataatagtta
    31561 cattactgac tttcgtgcac tcttgcaatg cttcattggt gacaaaacca ctctcagtca
    31621 gaaggcgata ggtaatcaac tcttgtcttt cttggggact atactcaact aacacttcaa
    31681 gttctgtcgg caaatcatcc aactctccag tcaaaatata gtatttccca tgcttcgtta
    31741 tttcgattcc atattgctcc atgctatctg tcaaatctga taaagttcga taaattgttc
    31801 tagatgaaac ctgtaagatt tcagtcatat cttgcattga aagcttccct acatgtagga
    31861 aagccttcaa taattgttct tctcgtttgg ttaatagcat tcgtaattta gaaacctcta
    31921 gttctatttg tacattctag cagccatttt gtcatattct ggtgttgtta ctaaactatc
    31981 cacaataaga aattgagaat tcggtgctgc ttgctgcact tctgcctgta aagaaatagt
    32041 tgttacaatc attatgtttt tagaattaaa ttcacctaat tctgaaattt tggcagtaga
    32101 aactggaata cgaatattct tgtttctaaa gatagcccga atcgtttcac agcccatagt
    32161 tgcagttccc tgtgctttac cataagcaac tactacagca tcaatatggt taaggtcaat
    32221 ttcctgacta tctactggtg ctgaagttgg tatatctcct tcaatttctg ctattggaaa
    32281 agctcctgtt aatgaagcta caatttcatc ataacgaggg gacgctaaga aattatcaac
    32341 agaaacatga atagcacttg gactcttgtc tttagctctt ggtgtcagtt cttcctgagt
    32401 aacaattaat gtttttggtg tatcaagcaa atttgagatt gcctgattag atactggaat
    32461 ctctagacct gcttttttaa ccttatctcg aagaatacta gctcccatag cagagcttcc
    32521 cataccagca tcgcaggcga aaatgatttt ttccactgag tctgtcgaaa taactgcatc
    32581 aacagaagtt gatactaact gacctttaga ctgagcttta gctgcttggg tagcctcttg
    32641 agcagcttcg agcgaatcct cagttgactt gtctgcatga agaataaggg ctgctacaaa
    32701 gaaagaaaca actgctgcca ctaaaacacc tagaagaaca tttagatggg gccaaacacc
    32761 ttttggcgcc gtagccataa tcgcaataat agaacctggt gaagctggag atttaagacc
    32821 agcgtctaag agttgaaaag taaaagttcc agagatacct cctgccatag cagctaaaaa
    32881 tagagtaggc ttcatcataa cataaggaaa gtaaatttca tgaatccctc cgaagaaatg
    32941 aataaccatt gccccccaag aagaagattt agcagaacct ttaccgaata cagcataagc
    33001 taatagaatt cccaggcctg gtccaggatt agcttccaat aggaagagaa ttgacttacc
    33061 agcttgagct acctgttcta ctcccagagg agtaaaaatg ccatgattga gggcattatt
    33121 gaggaaaagg actttagccg gttcgatgat aatattagct ataggaagga ggcgagcatt
    33181 gacaatagcc tcaacaccat tcccaacagc tccagtaaga gtcgatacga ctggaccgat
    33241 tgcgtagaaa gccaaaagca ataatgcaaa accaacgaga ccagctgaga agttattaac
    33301 taacatttca aatccgggac gaattttttc ctggaacttc tcatcaaatt tcttgatagt
    33361 ccatcctccc agtgggccca ttaccatagc tccgataaac ataggaacac tagaacctgt
    33421 gattgcacca acagtagcaa tagctcctac aacggcacca cgttggccat ggatcatata
    33481 tccacctgtg taaccaatca ggattggcaa taaatacgtt aacataggac caacaacagt
    33541 agctaactgt tcatttggca gatagccatc agcgataaag agggcagtca atactcccca
    33601 agcaataaat gctccaatat tgggcataac catatttgaa agcgatgtcc ctagtttttg
    33661 aaccctgact ttcaatgata ctttttcttc cattagatca tcctcctttt cttaaaacta
    33721 gtatatacaa agttaaaaac ctttaaaact aaagttttgt cacaaatttt gtgtcaaaac
    33781 attacttttg acgaggctgg gcaaaaaggc ttaaaaatga taaaacgcat catatccggt
    33841 gtac
//
LOCUS       DAVKQB010000021        33251 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKQB010000021 DAVKQB010000000
VERSION     DAVKQB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.021"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..157)
                     /locus_tag="VW528_000965"
     CDS             complement(<1..157)
                     /locus_tag="VW528_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911808.1"
                     /translation="MTPRYRAWIKTEKRMFFSDDILAIDYENEEIVTQQIYFENGLPD
                     DRDIYCYN"
     gene            complement(169..591)
                     /locus_tag="VW528_000966"
     CDS             complement(169..591)
                     /locus_tag="VW528_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911809.1"
                     /translation="MTETIKLPDYYEPDWKNARYGSLEELKELLLFKRIVKWDKDFLL
                     LEDGTKVTIEMSESDCCASAGGEFQDVSLDAVITNIEIGEPEEIPDHWGTGYKNKVTI
                     FHNQNPVAIANCEAEHNGYYYSVCSLVIGDIHFPVVNA"
     gene            complement(588..848)
                     /locus_tag="VW528_000967"
     CDS             complement(588..848)
                     /locus_tag="VW528_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEU9911810.1"
                     /translation="MRRFIAIWILVSAGLNIWHMDRIRDLEEKKPMVVYKADNAGAEI
                     FGRVVEKGRHGKLYTLTIRDYGVFVVTKDVYDKVKVGDEVLL"
     gene            complement(850..1188)
                     /locus_tag="VW528_000968"
     CDS             complement(850..1188)
                     /locus_tag="VW528_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911811.1"
                     /translation="MSYDLEILVKIESGDYICIAEPKYSSPTYNLGRMFRVAMNWDFD
                     QDTTYNIADVLDNIQRGISELERYPEKYVQYEPENRWGTVSVALEVLKSLKECILEQD
                     IDTKYLYMRW"
     gene            complement(1185..1373)
                     /locus_tag="VW528_000969"
     CDS             complement(1185..1373)
                     /locus_tag="VW528_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HEU9911812.1"
                     /translation="MSCSENLKKEKELTAAISNFKIEVLQNDDKLSSLSLSNIKGQAR
                     DLYECLVWLQYNAEESGR"
     gene            complement(1373..1876)
                     /locus_tag="VW528_000970"
     CDS             complement(1373..1876)
                     /locus_tag="VW528_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU9911813.1"
                     /translation="MNKQELIEKYKKLEGVWNAEGAELARQIFLQDLEQLDKPKPVKV
                     PQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPEWFYENNIEKFCLAWLDGYEVEKE
                     KRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELEDANFGWVFDCEG
                     IEIEGVE"
     gene            complement(1878..2195)
                     /locus_tag="VW528_000971"
     CDS             complement(1878..2195)
                     /locus_tag="VW528_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911814.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKSQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKDWLPKSKEFDDWVGYSPGMSQVVIAVYLIY
                     GGN"
     gene            complement(2220..2402)
                     /locus_tag="VW528_000972"
     CDS             complement(2220..2402)
                     /locus_tag="VW528_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911815.1"
                     /translation="MADYALYQGDVFVTLGTLAQISSETGITERMLKYYTYTSHQRRH
                     PNGRAVIKIEEEDNEN"
     gene            complement(2418..2849)
                     /locus_tag="VW528_000973"
     CDS             complement(2418..2849)
                     /locus_tag="VW528_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEU9911816.1"
                     /translation="MKLTLNIEPKPQSRPRFARRGSFTTTYEDKDMKTWRNHCQLLIA
                     NQYMGQPILEGALRARLRFYIKPPQYISKVKKNQQALLDEIIPVGKKPDIDNYEKALY
                     DSMSGIVFQDDGQIALHDVGKFYSLNPRIEVEIEVMKSLSI"
     gene            complement(2846..3175)
                     /locus_tag="VW528_000974"
     CDS             complement(2846..3175)
                     /locus_tag="VW528_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911817.1"
                     /translation="MASKINATERIAIIIEKQKIEVVTTLNYDMSISFDNKDTAPTLD
                     DNGDLFEPVYKCKVKAIPKNDVFFTSLTRVKSNIKTLQEVKKFFEFVNENRENLFEMA
                     GFKGALE"
     gene            complement(3189..3398)
                     /locus_tag="VW528_000975"
     CDS             complement(3189..3398)
                     /locus_tag="VW528_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911818.1"
                     /translation="MEWTDWVDWKPETKTDIKTKIENDGYTFPHYDKKNNGVKYVIST
                     LDIKRDCLRLGVPFEDVYPLQTTLF"
     gene            complement(3364..3870)
                     /locus_tag="VW528_000976"
     CDS             complement(3364..3870)
                     /locus_tag="VW528_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9911819.1"
                     /translation="MSKIKILDACCGSRMFWFDKNESHTIFMDIRQETFEIHDKKVNV
                     DPDIIGDFRDMPFEDNTFNLVVFDPPHLKWAGPNSIMKAQYGQLDKVTWSEDLAKGFE
                     ECLRVLKVGGTLVFKWSDRQINVKKLLEVIPFKPLFGQQRGTTHWLTFVKFEEDKNGV
                     DGLGGLET"
     gene            complement(3880..4296)
                     /gene="ssb"
                     /locus_tag="VW528_000977"
     CDS             complement(3880..4296)
                     /gene="ssb"
                     /locus_tag="VW528_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9911820.1"
                     /translation="MINNVTLVGRLVAPPDLRKTPNNVSSLQGTLAVNRNFKNENGER
                     EADFINFQAWRGTADIIAQYCSKGSLIGLTGRLQVRSYEKDGQRRYVTEVIAESVALL
                     ESRNSQHGQGNSFQNGNSSPFTDPNPFDLPNDGLPF"
     gene            complement(4391..4726)
                     /locus_tag="VW528_000978"
     CDS             complement(4391..4726)
                     /locus_tag="VW528_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HEU9911821.1"
                     /translation="MIKTVFFSCDYPHHEAIDDQINSWLAENPGIKLIDIKFQSNVSA
                     VADSGVSAEYWHTSALIIYKVPSENNISSINSNGLGFIISCEKCGSLSIIKGKDVGQN
                     VCYECKGEK"
     gene            complement(4719..5042)
                     /locus_tag="VW528_000979"
     CDS             complement(4719..5042)
                     /locus_tag="VW528_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911822.1"
                     /translation="MDIRKISDSVAIYSDGKKLHVIHNLGDEFILDFKVGEDSVWNLN
                     GQVVEIIDMIEPVFKVFSFCSKSGEGMQRLKHAIVHFEIFEQYIRDNQEDLMIWWHNP
                     GGEYD"
     gene            complement(5045..5206)
                     /locus_tag="VW528_000980"
     CDS             complement(5045..5206)
                     /locus_tag="VW528_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911823.1"
                     /translation="MTEIEKISGELAEYGVPDELIGKIEDLLATLYGEKKEIGDRKIL
                     DISPESMGR"
     gene            complement(5209..6249)
                     /locus_tag="VW528_000981"
     CDS             complement(5209..6249)
                     /locus_tag="VW528_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEU9911824.1"
                     /translation="MQELQVKVTQAQVEIIDREKFEQNINEVVAKYQNYTVTAATIKD
                     DKQTLADLRKLDKQVSDERIRNKKVLSEPADEFDKYVKNAIQPLKDIITKIAGDVKEF
                     EEHQKAVRIDTVKGYLANKSAEYMLDPRLFDEKALEYVKASDFMADGVTLKKATMKSL
                     DDMVTFEFQKQQEFEKAKSAISGLCAEYGMTDSPYIRQLKDLTLAEVFGQIKADYEFE
                     KQKEEIRPAQERAERESQELLAAQQTKQQEQAPKSTETPNFDPETGKILDGGQILQNE
                     PNALRGAENDLKRYAQKMTLEVYFVDTAEKDRFKAGLSQLGFDFKKNYQVRGYQRIEP
                     LTQAELNEQCGW"
     gene            complement(6259..7023)
                     /gene="bet"
                     /locus_tag="VW528_000982"
     CDS             complement(6259..7023)
                     /gene="bet"
                     /locus_tag="VW528_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEU9911825.1"
                     /translation="MTNNQLATQIKRDITTDPSLLTGADIKKYFDPQNLLTEKQVGQA
                     LALCKGRNLNPFANEVYIVAYQNNSGTEFSLIVSKEAFMKRAERCEGYDGFEAGITVM
                     RNGEMIEIEGSLKLPEDILIGGWAVVYRKDRSHRYKVTVDFNEYVKTDRNGNPRSTWK
                     SMPATMIRKTALVQTLREAFPDELGNMYTDIDGGDTFDAIKDVTPQESREDVVARKMA
                     QIDQFNKEQEANHADPEPTQNEDPIQGELLDGELEY"
     gene            complement(7035..7265)
                     /locus_tag="VW528_000983"
     CDS             complement(7035..7265)
                     /locus_tag="VW528_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911826.1"
                     /translation="MTQMTNKGKSFIRAEISEKQKEYIRLLAELRGVTTQELLGQVVE
                     RFIDRNLQLIQDYKNELDDLNSKSRRRIDMNT"
     gene            complement(7385..7528)
                     /locus_tag="VW528_000984"
     CDS             complement(7385..7528)
                     /locus_tag="VW528_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911827.1"
                     /translation="MTKIEIENRVWLLANHEEKNELLDLGLTSKARYVKRVLELGKVY
                     AHV"
     gene            complement(7515..7775)
                     /locus_tag="VW528_000985"
     CDS             complement(7515..7775)
                     /locus_tag="VW528_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911828.1"
                     /translation="MFKALKTIKKIKQLQKEMHDVSLAFLALQDVGLMPETERSKAKA
                     QTMHDVSHMLKDVLGGKSVDEAMKRLNSEVKIEEVEQEDDKD"
     gene            complement(7788..8042)
                     /locus_tag="VW528_000986"
     CDS             complement(7788..8042)
                     /locus_tag="VW528_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911829.1"
                     /translation="MYIISIYVKNTETGNEDFSLIGRDFLPTGHQDYIARVFGIKEEA
                     IDYLKSISYIASGVHGNDWVYQNEKLPEIESRCRIWKVGE"
     gene            complement(8043..8264)
                     /locus_tag="VW528_000987"
     CDS             complement(8043..8264)
                     /locus_tag="VW528_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911830.1"
                     /translation="MKLLTKLKLRLEGVIKSVNLDWREVAIEVSNDLLEERKRRFMRE
                     QENHDLKQELAAYKYKENFDIKARLQGEM"
     gene            complement(8264..8425)
                     /locus_tag="VW528_000988"
     CDS             complement(8264..8425)
                     /locus_tag="VW528_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911831.1"
                     /translation="MKTVKKEWEPRIVNIMADGSQVDDLTGYVIPAGHSYYDIILGMN
                     KQSNEEGVA"
     gene            complement(8497..9213)
                     /locus_tag="VW528_000989"
     CDS             complement(8497..9213)
                     /locus_tag="VW528_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEU9911832.1"
                     /translation="MNELINVTLNDNQEPVVSGRQLHEALGVNSRYTTWFDRMKEYGF
                     TEGQDFLPNLGKSTGGRQATDHIIKLDMAKEIAMIQRTERGKQVRQYFIQVEKDFNSP
                     EKIMARALLMADQKVHKLEAQIEADRPKVLFADAVSASHTSILVGELAKLISQNGYKI
                     GANRLFSWMRENGYLIKRKGSDWNVPTQRSMDLKLFEIMETNVQHADGHITVNKTPKV
                     TGKGQQYFINKFLNQEYLTG"
     gene            complement(9237..9440)
                     /locus_tag="VW528_000990"
     CDS             complement(9237..9440)
                     /locus_tag="VW528_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911833.1"
                     /translation="MRPRRYPYSGKKESTFVKADPELVEKLLRNTSFLERLQKKPINF
                     QIDLEEFKRLSYEAIHDTSQVTQ"
     gene            complement(9625..9903)
                     /locus_tag="VW528_000991"
     CDS             complement(9625..9903)
                     /locus_tag="VW528_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEU9911834.1"
                     /translation="MVVLSNKENWLVYPEEIARRLNISREMVLRHFKKIEKAGYLRTV
                     KKSLGRGRGVQTFRFFSDTKITDFQFEIMLQRLDEAIAMKKSELSTIT"
     gene            complement(10070..10306)
                     /locus_tag="VW528_000992"
     CDS             complement(10070..10306)
                     /locus_tag="VW528_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9911835.1"
                     /translation="MQELTKKQKLKKQELKPKIKLRKERKKHELTTVFMADLIGLKNR
                     RQYELKENGKAPFHDYEISIISNYFHKSESELFF"
     gene            complement(10498..10794)
                     /locus_tag="VW528_000993"
     CDS             complement(10498..10794)
                     /locus_tag="VW528_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911836.1"
                     /translation="MATNRTISVNTSEHDVLLTARKNHPAVFVDGMFLDGVERVEFTN
                     HYLEKCEVVLTFNDRVETNPFPLNDITLLEKLFGQSLNGQSLRDIVVQTLEDAD"
     gene            complement(10811..10981)
                     /locus_tag="VW528_000994"
     CDS             complement(10811..10981)
                     /locus_tag="VW528_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911837.1"
                     /translation="MKEKLNEFLKFRSQFTKREWFEINQAVEARLNQKADHLKLDDVD
                     LEIISKRLGRSI"
     gene            11180..11629
                     /locus_tag="VW528_000995"
     CDS             11180..11629
                     /locus_tag="VW528_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911838.1"
                     /translation="MNNFSFIETLAIAAIPAFVSGMWSYIAAKGNSKHEIDKINIAHS
                     QELENVENQFKQDMEKMQKQHSQELYSLQQTHELRLLELEKVSQLDTQTDQGLKINDL
                     IFKAISGEISADVAIKNMSTLSHFANKQQPSDLQKQFVKKLSKKNHK"
     gene            complement(11620..11787)
                     /locus_tag="VW528_000996"
     CDS             complement(11620..11787)
                     /locus_tag="VW528_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911839.1"
                     /translation="MNESFLTILGISMIASFITNLIAYLAGKHHLKKKIKNHKMWFDS
                     EIERIKKKYHL"
     gene            complement(11862..12137)
                     /locus_tag="VW528_000997"
     CDS             complement(11862..12137)
                     /locus_tag="VW528_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911840.1"
                     /translation="MNTLNEKAINIFKAVAKETLIQGTYEENFLYSQLEAFCTNCRQF
                     AFGWTELAEEIERQERYLLDSGFTQEEIDDIRFDAAFAGMLDKLNVA"
     gene            12298..13050
                     /locus_tag="VW528_000998"
     CDS             12298..13050
                     /locus_tag="VW528_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9911841.1"
                     /translation="MNILGSSIKEVRKSKKLTQKKLAELTGFKQNTISNHENGNRQLD
                     EKDIRIYAQALEVSPQYLFDLAKPSSIEIIPTTSPIQTIYDELEPPRQGKVLNYAKRQ
                     LKEQRNEEETKINEVSENIIRLDDYRQTTYRRVTGVVSAGSGSIQDDDLDMEVSFYED
                     EIPDDYDAIAYVVGNSMEPKIKNGDYLFIKNTQQVDYNTIGIFQVDGANYVKKLRQGY
                     LESLNPDYEDIHLDESNDIRTIGEVVSVYREK"
     gene            13052..13252
                     /locus_tag="VW528_000999"
     CDS             13052..13252
                     /locus_tag="VW528_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911842.1"
                     /translation="MSNESRPMEVIKHNLDCKCHRRREWIRVNDKWHAIEFSVDDPNE
                     PPMTEEEKANVALILQQHLSKE"
     gene            13435..14880
                     /locus_tag="VW528_001000"
     CDS             13435..14880
                     /locus_tag="VW528_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9911843.1"
                     /translation="MITTNKVAIYVRVSTTNQVEEGYSIDEQKDKLSSYCYIKDWNVY
                     KVYTDGGFSGSNTDRPALESLIKDAKKRKFDTVLVYKLDRLSRSQKDTLHLIEDVFIK
                     NGIEFLSLQENFDTSTPFGKAMIGLLSVFAQLEREQIKERMQLGKLGRAKSGKSMMWA
                     KTSYGYDYHKETGTVTINPAQALTIKFIFESYLRGRSITKLRDDLNEKYPKHVPWSYR
                     AVRTILDNPVYCGFNQYKGEIYPGNHEPIISKEEYDKTQSELKIRQRTAAENVNPRPF
                     QAKYILSGIAQCGYCGAPLKIMLGVKRKDGSRLKKYECHQRHPRTLRGVTTYNDNKKC
                     DSGFYYKDKLEAYVLKEISKLQDDADYLDKIFSGDNAETIDRESYKKQIEELSKKLSR
                     LNDLYIDDRITLEELQSKSAEFISMRGTLETELENDPALRKNKRKADMRKLLNAEKVF
                     SMDYESQKVLVRRLINKVKVTAEDIVINWKI"
     gene            complement(14898..14960)
                     /locus_tag="VW528_001001"
                     /pseudo
     CDS             complement(14898..14960)
                     /locus_tag="VW528_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
     gene            complement(14962..15978)
                     /locus_tag="VW528_001002"
     CDS             complement(14962..15978)
                     /locus_tag="VW528_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9911844.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(15926..16867)
                     /locus_tag="VW528_001003"
     CDS             complement(15926..16867)
                     /locus_tag="VW528_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9911845.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(16943..17308)
                     /locus_tag="VW528_001004"
     CDS             complement(16943..17308)
                     /locus_tag="VW528_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9911846.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(17459..18517)
                     /locus_tag="VW528_001005"
     CDS             complement(17459..18517)
                     /locus_tag="VW528_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9911847.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(18680..19831)
                     /gene="nagA"
                     /locus_tag="VW528_001006"
     CDS             complement(18680..19831)
                     /gene="nagA"
                     /locus_tag="VW528_001006"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9911848.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(19984..21801)
                     /locus_tag="VW528_001007"
     CDS             complement(19984..21801)
                     /locus_tag="VW528_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9911849.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTV
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(21902..23044)
                     /gene="tgt"
                     /locus_tag="VW528_001008"
     CDS             complement(21902..23044)
                     /gene="tgt"
                     /locus_tag="VW528_001008"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9911850.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            23174..24031
                     /locus_tag="VW528_001009"
     CDS             23174..24031
                     /locus_tag="VW528_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9911851.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(24058..24701)
                     /gene="pcp"
                     /locus_tag="VW528_001010"
                     /pseudo
     CDS             complement(24058..24701)
                     /gene="pcp"
                     /locus_tag="VW528_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(24808..25176)
                     /locus_tag="VW528_001011"
     CDS             complement(24808..25176)
                     /locus_tag="VW528_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9911852.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELA
                     HPSVSLLFKNQGIYKALLGVFLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            complement(25187..25612)
                     /locus_tag="VW528_001012"
     CDS             complement(25187..25612)
                     /locus_tag="VW528_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9911853.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            complement(26040..27218)
                     /locus_tag="VW528_001013"
     CDS             complement(26040..27218)
                     /locus_tag="VW528_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9911854.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEGQPAETTSPVEEVAPQATTLAEKQETQVSSQTESAVEATTMP
                     VEEKATETTATSSEAKEVASSNGATAAVSTYQPEETKTISTTYEAPAAPDYAGLAVAK
                     SENAGLQPQTAAFKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPESSELGDKIA
                     EYAIQNMASRGISYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG
                     "
     gene            complement(27384..28004)
                     /locus_tag="VW528_001014"
     CDS             complement(27384..28004)
                     /locus_tag="VW528_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9911855.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(28008..29288)
                     /locus_tag="VW528_001015"
     CDS             complement(28008..29288)
                     /locus_tag="VW528_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9911856.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(29349..30833)
                     /locus_tag="VW528_001016"
     CDS             complement(29349..30833)
                     /locus_tag="VW528_001016"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9911857.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALRERLAA
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNMQGQYKLTDFDAEILELFAAEYATEEETAAEIKRVYELDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTTVAAADMQAAVEAYLGL"
     gene            complement(30909..32186)
                     /locus_tag="VW528_001017"
     CDS             complement(30909..32186)
                     /locus_tag="VW528_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9911858.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(32293..32376)
                     /locus_tag="VW528_001018"
     tRNA            complement(32293..32376)
                     /locus_tag="VW528_001018"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32340..32342),aa:Leu,seq:caa)
     gene            complement(32386..32457)
                     /locus_tag="VW528_001019"
     tRNA            complement(32386..32457)
                     /locus_tag="VW528_001019"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32423..32425),aa:Gln,seq:ttg)
     gene            complement(32468..32540)
                     /locus_tag="VW528_001020"
     tRNA            complement(32468..32540)
                     /locus_tag="VW528_001020"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32505..32507),aa:His,seq:gtg)
     gene            complement(32555..32625)
                     /locus_tag="VW528_001021"
     tRNA            complement(32555..32625)
                     /locus_tag="VW528_001021"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32591..32593),aa:Trp,seq:cca)
     gene            complement(32630..32710)
                     /locus_tag="VW528_001022"
     tRNA            complement(32630..32710)
                     /locus_tag="VW528_001022"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32674..32676),aa:Tyr,seq:gta)
     gene            complement(32723..32795)
                     /locus_tag="VW528_001023"
     tRNA            complement(32723..32795)
                     /locus_tag="VW528_001023"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32760..32762),aa:Phe,seq:gaa)
     gene            complement(32799..32872)
                     /locus_tag="VW528_001024"
     tRNA            complement(32799..32872)
                     /locus_tag="VW528_001024"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32836..32838),aa:Met,seq:cat)
     gene            complement(32883..32972)
                     /locus_tag="VW528_001025"
     tRNA            complement(32883..32972)
                     /locus_tag="VW528_001025"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32934..32936),aa:Ser,seq:tga)
     gene            complement(33055..33128)
                     /locus_tag="VW528_001026"
     tRNA            complement(33055..33128)
                     /locus_tag="VW528_001026"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33092..33094),aa:Ile,seq:gat)
     gene            complement(33163..33233)
                     /locus_tag="VW528_001027"
     tRNA            complement(33163..33233)
                     /locus_tag="VW528_001027"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33199..33201),aa:Gly,seq:tcc)
BASE COUNT         9641 a         7506 c         5891 g        10213 t
ORIGIN      
        1 gattgtaaca atagatatct ctatcgtctg gtaaaccatt ctcaaaataa atttgttgtg
       61 tcactatttc ttcgttttcg tagtcaatag caagaatgtc atctgaaaaa aacatacgtt
      121 tttctgtttt tatccacgct ctatatcttg gtgtcatgtt aaatcctcct aagcattaac
      181 aactggaaaa tgaatatcac caatcactaa agagcatacg ctgtaataat agccgttatg
      241 ctctgcttca caattggcaa tagctacagg gttctgatta tggaagatag ttactttgtt
      301 tttataacca gttccccaat ggtcggggat ttcttccggt tctccaattt caatattagt
      361 aatcacagcg tcaagtgata catcttggaa ctccccacct gctgaggcac agcaatcact
      421 ttcagacatt tcaatagtga cctttgtgcc gtcttcaagc agcaaaaagt ccttatccca
      481 tttcacaata cgcttaaaga gcaacaattc tttaagctct tccaacgacc cgtaccttgc
      541 atttttccaa tcaggctcat aatagtctgg tagtttaata gtttctgtca tagtaacacc
      601 tcatccccaa ctttcacttt atcgtacacg tccttcgtaa ccacgaacac accatagtca
      661 cgtatcgtta gcgtatatag cttcccatgc cgtccttttt caacgacacg cccgaatatc
      721 tcagcgccag cgttatcagc tttatagaca accatcggct ttttctcttc caaatctcga
      781 atcctgtcca tatgccagat gttcaatccg gcagagacaa gaatccagat agctatgaat
      841 cgtctcatat taccacctca tatataaata tttcgtatca atatcttgtt ctaaaataca
      901 ctctttcagt gactttaaaa cctccaatgc aacgctaact gttccccatc tattttcagg
      961 ttcatactgc acatactttt cagggtaccg ttctagttca gagataccgc gttgaatgtt
     1021 atctaaaaca tcagcgatgt tgtacgtagt gtcttgatca aaatcccagt tcatagcaac
     1081 tctaaacatt cttccaagat tgtaggtcgg agaactatat ttaggttcag cgatacaaat
     1141 ataatctcca ctctctattt ttactaatat ttccaaatca taactcatct acctgattcc
     1201 tccgcattat actgcaacca tactaagcat tcatatagat cccttgcttg ccctttgatg
     1261 ttgcttaatg atagactgct caatttatca tcattttgta agacttctat cttgaaattt
     1321 gaaatagcag cagtcaattc tttttctttt tttaaatttt cactacatga catcactcca
     1381 ccccctcaat ctcaattcct tcacaatcaa acacccagcc gaagtttgcg tcttctagtt
     1441 ctttacgggt gtgggaatat ataacatcgt ctaaactaaa gctttttgta aagaaatacc
     1501 ttttcaaaag ttctccataa accaacatat tttctttaat attcccttta atcttaacaa
     1561 aataccgctt ctctttttca acctcgtagc cgtcaagcca agcaagacag aatttttcga
     1621 tgttattttc gtaaaaccac tcaggaactt tcttatcata atgatcttca attactctca
     1681 ttgcaccgta aacatgaaaa ttgtttttct ttttaaattc tatatattcc gccacacact
     1741 gcggaacttt gactggtttt ggtttatcta gttgttccaa gtcttgcaga aaaatttgac
     1801 gagctagttc tgctccttca gcattccata caccctcaag ttttttatat ttctcaatca
     1861 attcctgctt attcatctta gtttcctcca taaatcaaat aaactgcaat aactacctga
     1921 gacattcctg gcgaatagcc aacccaatca tcaaactcct tagattttgg caaccaatcc
     1981 ttagtagctc ccaaatcata gtctgtaggt ttttcatcag cgaagatgca ttccatcgct
     2041 cccataaacg tcataccatc ttctgccatt tcccaaaaat agtccgcccg gtctttcacc
     2101 gcttgtggta aatcttgctt gggaggttgc gacttcccgt cttctaccgt ccagttgtat
     2161 acttcattaa ctttttgctt taactcttcc atcatcttcc aacttcttca ctttccgtct
     2221 taattctcat tatcttcctc ctcaatttta ataacggccc taccgtttgg gtgtcgtctt
     2281 tggtgtgatg tgtaagtgta atactttaac atcctttcag taattcctgt ctcgctactg
     2341 atctgcgcta atgttccaag cgtaacaaac acatcacctt gatataatgc gtagtcagcc
     2401 atctgctcct catttcttca aatactcagg gatttcatga cctcaatctc aacctctatc
     2461 cgtggattta gactgtagaa cttgcctaca tcatgtagcg ctatctgacc gtcgtcctgg
     2521 aagacgatcc ctgacatgct atcatatagc gctttttcgt agttatctat gtcaggcttt
     2581 ttgcctactg gtataatttc atccaggagg gcctgttggt tcttcttgac tttagaaatg
     2641 tactgaggag gtttgatata aaatctaagc cgtgccctca aagctccctc aaggataggc
     2701 tgacccatgt actgattagc aatgagcagc tggcaatgat tgcgccatgt tttcatatcc
     2761 ttgtcttcgt aagttgtggt aaaactccca cgtcttgcaa accttggccg tgattgaggt
     2821 ttaggctcaa tgttcagggt caatttcatt caagagcccc cttaaatcct gccatctcaa
     2881 agagattttc tctgttttcg tttacgaact caaagaattt tttaacctct tgtagcgtct
     2941 tgatgttgct cttgactcgt gttaatgagg tgaaaaatac atcatttttg ggaattgcct
     3001 taactttgca cttgtagacc ggttcaaaaa ggtcaccatt gtcatctagt gtaggagccg
     3061 tgtctttgtt atcaaagcta atgctcatat catagtttag agtcgtaacg acctctattt
     3121 tttgtttctc aatgatgata gcaatacgtt ctgtcgcatt gattttactt gccatgttct
     3181 ttctcctgtt aaaaaagtgt cgtttgcaaa gggtacacat cttcaaacgg tactccaagt
     3241 cttagacagt ctcgtttgat gtccagtgta gagatgacgt acttgacgcc gttgtttttc
     3301 ttgtcatagt gtggaaaagt gtacccgtca ttttcaattt tggttttaat gtccgttttg
     3361 gtttcaggtt tccaatccac ccaatccgtc cactccattc ttgtcctcct caaactttac
     3421 aaacgttagc cagtgtgtgg tgcctctttg ctgaccaaat aagggcttga atggtatcac
     3481 ctctagtaat ttctttacat ttatctgacg gtcagaccat ttaaagacta gtgtgccgcc
     3541 aacttttaga actctcagac attcttcaaa acccttggcc aaatcttccg accaggtaac
     3601 tttgtccagc tgtccatact gagctttcat tatcgaatta ggtccagccc attttagatg
     3661 tggtggatca aacacaacta gattaaatgt gttgtcttca aaaggcatgt cacgaaaatc
     3721 accgataata tcagggtcta cattgacctt tttgtcatgt atctcaaatg tttcttgcct
     3781 aatgtccata aaaattgtgt gactttcgtt tttatcaaac caaaacatac gactgccaca
     3841 gcaagcgtca agaattttaa tttttgacat atatacctcc taaaacggca aaccgtcatt
     3901 tgggaggtca aaggggttag gatcggtaaa aggtgagcta ttcccatttt ggaaactgtt
     3961 gccttgtccg tgctgactgt tgcgactctc tagcagagct acactctcag cgattacttc
     4021 agtcacatat cgacgctgac cgtctttctc gtaagaccta acttgtaagc gccctgtgag
     4081 gccaataagt gagcccttgc tgcaatactg agcaatgatg tcagctgtac ctctccaagc
     4141 ttgaaaattg ataaaatcag cctcacgctc tccattttcg tttttgaaat tgcgattgac
     4201 tgcaagcgtg ccctgtaaac tagatacatt attaggcgtt tttcgtagat caggaggcgc
     4261 tacaagcctc ccaaccagtg tgacgttatt gatcatctga tttgtccccc tctagtgcta
     4321 cgctctccca agagataccc taaaaacatc cataggatag ccatcccaat ctctttgata
     4381 aaatcattca ttatttctct cctttgcatt cataacatac attttgacct acatcttttc
     4441 ccttgattat tgataagcta ccacatttct cacagctgat tatgaaacct aaaccatttg
     4501 aattaatact gcttatattg ttctctgagg gaactttgta aataatcaat gcggatgtat
     4561 gccaatattc agcactgact ccactgtcag cgacagcaga cacatttgat tgaaatttga
     4621 tgtcaatcaa cttaatgcct ggattttcgg caagccagct atttatttgg tcgtcaatcg
     4681 cctcatgatg tggataatca catgaaaaaa atacggtttt aatcatattc ccctcctgga
     4741 ttgtgccacc agatcatcag gtcttcctga ttatctctga tgtactgctc aaatatttca
     4801 aagtggacga tagcatgttt taagcgttgc ataccctctc cagattttga gcaaaagctg
     4861 aaaactttaa agacaggctc aatcatgtca ataatttcta cgacttggcc attgaggttc
     4921 cagacgctat cctctcccac cttaaaatct aggataaact catcccctag gttgtggata
     4981 acatgcaatt tcttgccgtc cgagtagatg gctacgctgt cagatatttt tctgatgtcc
     5041 atggttacct ccccattgac tctggagaaa tatccaagat ttttctatct ccaatttcct
     5101 ttttttcgcc atacagagtc gctaataggt cctctatttt tcctattaac tcatcaggca
     5161 ccccatattc agccaattct cctgaaattt tttcaatttc tgtcatactt accacccaca
     5221 ttgttcattt agttcagcct gagttaatgg ctcgatacgt tgataacccc tgacttgata
     5281 gttcttttta aaatcaaatc cgagttgact tagaccagcc ttgaaacggt ctttttcggc
     5341 tgtgtctaca aaatacacct ctaaagtcat tttttgggca tatcgtttta ggtcattttc
     5401 agcccctcta agagcgttag gctcattttg gaggatttgt ccaccgtcca agattttgcc
     5461 cgtttctggg tcaaaatttg gggtttccgt tgattttgga gcctgttctt gctgtttggt
     5521 ttgttgggct gctaaaagtt cctgactttc tcgctctgct cgttcttgag ccggtctgat
     5581 ttcttccttt tgcttttcaa actcataatc agctttgatt tgtccaaaga cttcagcaag
     5641 agtcaagtct ttcagctgtc taatgtaagg tgagtcagtc atgccatact cagcacataa
     5701 ccctgaaata gctgacttgg ctttttcaaa ttcttgttgt ttctgaaatt caaatgtgac
     5761 catgtcatca agtgacttca tagtggcttt tttaagcgtc acgccatctg ccataaaatc
     5821 gctagctttg acatactcaa gggccttttc atcaaagaga cgaggatcca gcatgtactc
     5881 agctgatttg ttggctaggt agcctttgac tgtgtcaatt cggacagcct tttgatgttc
     5941 ttcaaactct ttgacatcac cagcaatttt ggtaatgatg tcttttagag gctggatggc
     6001 attcttgaca tacttgtcaa attcatcagc tggttcagat aagactttct tattcctgat
     6061 ccgttcatca gaaacctgct tgtctaattt tcgtagatcg gcaagtgtct gcttgtcatc
     6121 cttgatagtt gcagccgtaa ccgtgtaatt ttgatacttt gctacaacct cattgatatt
     6181 ctgctcaaat ttctcacggt caatgatttc aacctgtgct tgtgttactt ttacctgtaa
     6241 ttcttgcatg ttgtcctcct aatattcaag ttcaccgtct agcaactcgc cctggattgg
     6301 atcctcattt tgagtaggtt caggatctgc atgatttgcc tcttgctctt tgttgaattg
     6361 atcaatctga gccatcttgc gtgctacgac atcctcacgg ctctcttgag gtgtgacgtc
     6421 tttgattgcg tcaaatgtat ctccaccatc gatgtctgta tacatgttcc caagttcgtc
     6481 agggaaagcc tctctaagag tctgcactag agctgttttt ctgatcatag tggctggcat
     6541 tgatttccaa gtgctccgtg gattgccatt tctgtctgtt ttgacatact cattaaagtc
     6601 aactgtgacc ttatatctgt gtgaacggtc tttacgatag acaacggccc aaccacctat
     6661 tagaatgtcc tcaggtaatt taagagagcc ctcaatctct atcatttctc catttctcat
     6721 gacagtaatt ccagcctcaa aaccatcata tccctcacaa cgttcagcac gtttcataaa
     6781 tgcctctttt gagacaatca agctgaactc tgtgccgcta ttgttttgat aggctacaat
     6841 gtagacctcg ttagcaaatg ggttaagatt gcgaccttta cacaaggcta gagcctgacc
     6901 tacttgtttt tcagtcagta ggttttgtgg gtcaaaatac tttttgatgt ctgccccagt
     6961 caataaactt ggatcagtag tgatgtcacg ttttatctgt gttgctaatt gattattagt
     7021 catcttgttt tctcctatgt gttcatgtca attctgcgtc tagacttact atttaagtca
     7081 tctaattcat ttttgtaatc ttgaataagt tgcaaattcc ggtcaatgaa gcgttctaca
     7141 acttgaccta gaagttcttg tgttgtcacg cctctcaatt cagcaagaag tctgatatat
     7201 tctttttgtt tttcagagat ctctgctctt atgaacgact ttcctttatt ggtcatctgc
     7261 gtcatttttt tctcctttag gctagcaatc tcctacatag atccattgac cagcgctgaa
     7321 aatccaatca gctgggtcaa gttcttctcg ttcttcaggc ggttgcatta tatctctgtc
     7381 ataattaaac atgagcatac accttcccaa gctccagaac tcgtttcaca tatctagcct
     7441 tggatgttag cccaagatcc agcaattcgt ttttttcttc atgattggcc aaaagccaga
     7501 cacggttttc aatttcaatc tttgtcatct tcctgctcca cctcttcaat tttcacttcg
     7561 ctatttagac gtttcatggc ttcatctacc gacttgccgc ccaggacgtc cttgagcatg
     7621 tggcttacat cgtgcattgt ttgagccttc gccttgcttc tttcagtctc tggcatcaat
     7681 ccgacatctt gtagagccag aaaggctaaa ctgacatcgt gcatttcttt ctgaagctgt
     7741 ttgatttttt tgattgtttt tagtgcttta aacatattgt tctcctttta ttctcctact
     7801 ttccaaattc ggcaacgtga ctcaatttct ggtagttttt cattttgata aacccaatcg
     7861 ttaccatgaa cacctgatgc gatgtaagat atagatttta agtaatcaat cgcttcttct
     7921 tttattccaa aaactcttgc aatatagtct tggtgccccg tcggtaagaa atcacgtcca
     7981 atcaaactga aatcctcgtt tccagtttca gtattcttga cataaatcga tataatgtac
     8041 atctacattt ctccttgcag tctagccttg atatcaaagt tttctttgta cttgtaggca
     8101 gcaagctcct gcttcaagtc atggttttct tgctcacgca taaagcgacg tttgcgctct
     8161 tcgagaaggt cattgctaac ctcaatagca acctctctcc agtcaaggtt gactgattta
     8221 ataacacctt cgagtctgag ttttaattta gtaagtaatt tcattaagct acgccctcct
     8281 cgttagattg cttgttcatg cctaaaataa tgtcatagta cgaatgacca gcagggatga
     8341 catagcctgt caaatcatca acttgagaac catctgccat gatgtttaca atccgtggct
     8401 cccattcctt ttttactgtt ttcatgatat aattacctcg taaagtattt tgcttagtcc
     8461 ctcaatggaa ttgccgttcc agaggggctt ttgtttttat cctgtcaagt attcctgatt
     8521 aaggaactta ttgataaaat actgttgtcc tttgcctgtg acctttggtg tcttgttcac
     8581 agtgatatgt ccatctgcgt gttgcacgtt tgtttccatg atttcaaaga gtttcaagtc
     8641 catgctacgt tgggttggca cgttccaatc tgagcctttg cgcttaatca ggtagccatt
     8701 ttcgcgcatc caagaaaaga ggcgattggc accgattttg tagccgtttt ggctaatgag
     8761 cttggcaagt tcgccaacca agatagatgt atggcttgca cttactgcgt ctgcaaatag
     8821 caccttagga cggtcagcct caatctgagc ctctagcttg tggactttct gatcagccat
     8881 gagcaatgct cttgccatga ttttctcagg gctattaaag tctttttcta cttggataaa
     8941 gtattgtcgg acttgcttgc ctctctccgt ccgctggatc atagcaattt ccttggccat
     9001 gtctagcttg atgatgtggt cagtagcttg tcgccctcct gtactttttc ccaaatttgg
     9061 gagaaagtcc tgaccttctg taaatccgta ttccttcata cggtcaaacc atgttgtata
     9121 tcttgaatta acacccagcg cctcatgtag ttgtcttcct gacactactg gctcctgatt
     9181 gtcattcagg gttacgttga tgagttcgtt catagtattc cttttattcc ttcctcctat
     9241 tgagttactt gagaagtatc atgaatggct tcatagctaa gacgcttaaa ttcttctaag
     9301 tctatctgaa aattgatagg ctttttttgt aaacgctcaa gaaaactagt gtttcttaaa
     9361 agtttttcaa ctaactcagg gtcggccttt acaaaggtgg actctttttt cccactatac
     9421 ggatatcgtc ttggtctcat ttcctcacct cctttgtatt tattttttct accctctctt
     9481 ttatttagag aagtaggact ggttgtcttt taatatttat tgttatttaa tacttgttgt
     9541 tagttaatat ttattagtgc cttattttac aacgttgtaa aatgcaatgt tgtaaaatac
     9601 aatgttgtaa aatgcaactt tgtattaagt aattgtggat aactcagact tcttcatagc
     9661 tatcgcttca tcaagacgtt gcaacataat ttcaaattga aaatcagtta tttttgtatc
     9721 tgagaagaat ctgaaagtct gaactcctct ccctctgccg aggctttttt tgacagtcct
     9781 taaatatcca gctttttcaa tctttttgaa atgccttaaa accatttcgc ggctaatatt
     9841 caaccgtcta gctatttcct ctggatagac aagccaattc tctttattgc tgagaacgac
     9901 catcaatatc ccaattgttg ccggctcaag ctttggatct ctcagaaaat catttttgac
     9961 tgcagtgtaa tcatccgtcg catttctgaa agattaattg aagattcaag tttttaaaat
    10021 ctgtcataag ttctcctttc ttttaaatct caaattgaga tattttattt taaaaaaata
    10081 attcactctc tgatttgtga aaataattag aaataataga tatctcataa tcatggaatg
    10141 gagctttgcc attttctttt aattcatatt gtctgcgatt tttcaaacca attaaatctg
    10201 ccataaaaac tgtcgtaagt tcatgcttct ttctctcttt tctaagcttt attttcggct
    10261 tcaattcttg cttttttaac ttttgttttt ttgtaagttc ctgcacgcac tcaccccctt
    10321 gtgttaatag ttaccctccg tgatttctgc tataatgtag tcagaaagga gataatgtta
    10381 tgactgatca ccagctagaa acttctttaa tcgtccttgg caaagaattt gatagaacca
    10441 agaaaaacgg aaaagaaagt tttagtgttc atgtttcttt ttttgatggt ttagatgcta
    10501 atcagcatct tcaagagttt gcacgacaat atcccgtaaa gattgaccgt tcaaactctg
    10561 accaaataac ttttctaata aagtaatatc gtttagaggg aagggattgg tttcaactct
    10621 atcgttaaac gttaaaacaa cttcacactt ctccagataa tgattggtaa attccactcg
    10681 ctcaactccg tcgagaaaca ttccatcgac gaatacagca gggtggtttt ttcttgccgt
    10741 caacaataca tcatgctctg atgtatttac tgatatagtt ctgttagttg ccatttgttt
    10801 caccctttct ttagatagat cgtcctagtc ttttagagat gatttctaaa tctacgtcgt
    10861 ccagtttcaa gtggtcggct ttttgattta aacgagcttc gacagcttgg ttgatttcaa
    10921 accattcacg ttttgtaaac tggcttctga attttagaaa ttcgtttagt ttttctttca
    10981 taccctcgtc ctactttcca ttgccctgag ttctatctca tgactgactt gaagaaatag
    11041 cttctcacac gctattttag cttctctgta cgttgtgttt tcgctgatga aataatcagc
    11101 aagttcgatg attttatctt ccaattctaa ctacctttca aatgtggtat aatcaaaata
    11161 aaatgattgg agaaatctta tgaataattt tagttttata gaaacacttg cgatcgctgc
    11221 catacctgcc tttgtttctg gcatgtggtc ttacatcgct gctaaaggaa acagcaagca
    11281 tgaaattgat aaaattaaca ttgcacactc acaagagctt gaaaatgtcg aaaatcaatt
    11341 taaacaagat atggaaaaaa tgcaaaagca acactcacaa gaactttatt cgctgcaaca
    11401 aactcacgaa ttaagattac ttgaacttga aaaagtatct caactagaca ctcaaaccga
    11461 ccaaggatta aagataaatg acctaatttt taaagctatt tcaggagaaa tatctgcaga
    11521 tgtagcaata aaaaatatga gcactcttag tcatttcgcg aacaagcaac aaccttccga
    11581 tcttcaaaaa caatttgtga aaaaattatc caagaaaaat cacaaatgat actttttctt
    11641 gatacgctct atctcagaat caaaccacat tttgtggttt ttaattttct ttttcagatg
    11701 atgcttacca gctaagtaag caattaaatt cgtgataaaa ctagcaatca ttgatatgcc
    11761 tagtatcgta agaaaagatt cattcatcgt tctactcctt tctctctttt ttcgctctat
    11821 gagcaacaac ctgccaagga ttcgaacctt ggtgatacca atcaggctac atttaattta
    11881 tcaagcattc ctgcaaatgc tgcatcaaaa cgaatgtcat cgatttcctc ttgagtgaaa
    11941 ccagaatcga gaaggtaacg ctcttggcgt tcaatctcct ctgctaactc tgtccatcca
    12001 aaagcgaatt gacggcagtt agtacagaat gcttcaagct ggctgtaaag gaagttttcc
    12061 tcgtaagtgc cttggattaa agtttcctta gctactgctt tgaagatgtt gattgctttc
    12121 tcgtttaatg tgttcatggt gtttccctcc ggtttgtttt tgttatttcc ttaagcttga
    12181 tttaattata tctcatcttg agatatttgt caataatttt ttatcacttt ttgagatttt
    12241 tttatttatt ttttggtcgc gttagtatat aataagaaat gaaaggagtt gtttaatatg
    12301 aatatactag gtagctcaat taaagaagta agaaaatcca agaaattaac tcaaaaaaaa
    12361 cttgccgagc tgacaggttt taaacaaaat acaatttcta accatgaaaa cggaaataga
    12421 cagttagatg aaaaagatat tagaatatac gctcaggctt tagaagtttc tcctcaatat
    12481 ctatttgacc tagccaaacc ttcatctatt gaaattatcc ctaccacttc cccaatccaa
    12541 accatctacg acgaactaga accacctaga cagggcaaag tcctgaatta tgcaaagagg
    12601 caactgaaag agcagaggaa cgaagaagaa acgaagataa acgaagtatc ggagaacatc
    12661 atcagactgg acgactacag acagactact taccgacgtg ttactggggt tgtctctgct
    12721 ggtagtggtt cgatacagga cgacgattta gatatggagg tttcgttcta tgaggatgaa
    12781 atcccagacg actacgacgc tatcgcttat gtcgtcggca actctatgga gccaaagata
    12841 aagaatggtg actatctttt tataaagaat acccaacagg ttgattataa cactatcggc
    12901 atcttccaag tagacggcgc taactatgtt aagaaactgc gtcagggata tctggaaagc
    12961 ttgaatccag attatgagga tatacaccta gacgaaagca acgacatccg aactattggg
    13021 gaagttgtca gtgtgtatag ggagaaataa catgagtaac gaaagtagac cgatggaagt
    13081 gattaaacac aacctagatt gcaaatgcca cagacgaaga gagtggatta gagtaaatga
    13141 taaatggcat gctatcgagt tttcggtaga cgatccaaac gaacctccta tgacagagga
    13201 agagaaagcc aacgtggcct taattcttca acaacactta tcgaaagaat aaaaccaact
    13261 gtttccaaaa tggaaataat tgcaaacaaa aaaaagcccc acgctctcaa aactttggcg
    13321 agtctgagcg tgaggcatgt gacaggaaaa gattttcatg gagataacct ctcatgatgt
    13381 cttttcttgt acccatttta tcatttttta ggaaattttg aaagaggtac tactatgata
    13441 acaacaaata aagtagctat atatgtcagg gtatcgacga caaaccaggt tgaggaggga
    13501 tactctatag atgagcaaaa agacaagctc tctagctact gctacattaa agactggaat
    13561 gtatacaaag tatatactga tggaggtttc tcaggatcca atactgacag accagcgcta
    13621 gaaagtctta tcaaagacgc taaaaaaaga aaatttgaca cagttctagt ctataagctg
    13681 gaccgtctta gccgtagtca aaaagacacg cttcacttga ttgaggatgt attcatcaag
    13741 aatgggattg aatttctgag cttgcaggag aactttgaca cctctactcc ttttggtaag
    13801 gctatgattg gactcttgag cgtctttgct cagctagaaa gggagcaaat caaggaacgc
    13861 atgcaacttg gcaagctagg acgtgccaaa tctggtaaat ccatgatgtg ggctaagaca
    13921 tcctatggtt acgattacca caaagagaca ggcacagtga ccatcaatcc agctcaggct
    13981 ctgaccatta agtttatctt tgagagttac ctgagaggga gatctattac taagttgaga
    14041 gatgatctaa atgagaaata cccaaagcat gtgccttgga gctatcgggc ggtcagaacc
    14101 atactcgata accctgtcta ttgtggtttc aatcagtata agggagaaat ttatccaggt
    14161 aatcatgagc cgattatttc aaaagaggaa tacgataaga ctcaatctga gctaaaaata
    14221 agacaaagaa cagcagcaga gaatgtcaat cctagaccat tccaagctaa gtacatttta
    14281 tccggtatcg cccaatgtgg atattgtggc gctcctttaa aaattatgtt aggcgtaaag
    14341 aggaaagatg ggagcaggtt aaaaaaatat gaatgccacc aaaggcaccc acgaacgctg
    14401 agaggcgtta ctacctacaa cgacaataaa aagtgtgact caggatttta ctacaaagac
    14461 aagctagagg cctatgtgct aaaagaaata agcaaactac aagatgacgc tgattacctg
    14521 gacaaaatat tttcaggaga caatgctgag accatagacc gtgagagcta taagaaacaa
    14581 atagaggagc tatcaaagaa actgagcaga cttaacgacc tctacataga cgaccgtatc
    14641 acgctggagg aattacagag caagtcagcc gaatttataa gcatgagggg gactcttgaa
    14701 actgaactag aaaacgatcc agcactcagg aagaacaaaa gaaaggctga tatgaggaaa
    14761 ctgctaaacg ctgagaaagt cttttcaatg gactacgaaa gtcaaaaggt gcttgttaga
    14821 aggcttataa acaaggttaa ggtgacagct gaggacattg ttatcaattg gaaaatataa
    14881 ataattttag taacctacat ttccaccaat gtaaaagctt taaccttagc ttttttcaag
    14941 aatgtcatca tttttttcat tttaaaaatt tacctccata ttttgataca tgggcatgag
    15001 cattgccgca taaagtaaaa cgataatcag tgccacaaag ataaaaacca gtggctgcac
    15061 caaattcatg gtgcggttga ctcgggtaaa aaaggcttcc caagtttttt cagcatagat
    15121 ttccaactca ctacccagct tggacttaac ttccccatac tctatgatga gactcaattc
    15181 cttcctaaag aaaggatagg ttcctatcgt ctgagaaaat tcacggccat ttttcagggt
    15241 ttgagccaga tcttgaccga tttctttaaa gagctgggaa ccttgttcct gcatcatttg
    15301 aaaaatctgc gtcagctcca ttccctgtga aatcatattc ccccattcac gtgcataata
    15361 ggctgtcaag taggtctgca caaagattcc aataaaggga aggcgtgcta agatagaaaa
    15421 gacactcatc ttagaacttc ttttataaaa agttagtgct aaaagggcaa gcacggaaac
    15481 aagccctacc atgcctagaa aaatttgtgg cagattaccg ataattcggg tggcaatatt
    15541 gctactatcc agttgtggga gcaggtaatt ccgtagcccc agcataatta agagaagaaa
    15601 acccagcaaa atcaaaggat aggtcgctac ttcaattaac tttttcttga ccttggccag
    15661 attgtctaga tattcttcta tctttcccaa actcaggtga agattgccat gaacttcagc
    15721 tagagataac tgagtgacaa tggtacttga acatcccaaa ctttccatca tttctgagaa
    15781 tgatttcccc tgagacaagc ccacacgcat ctgggtcaca cactgcttgt ccaacaaagc
    15841 actcctatct aaaaaggaga tggtctccac cagatgaaaa ccgctagaaa agagattgtt
    15901 aaatagggtg atgatatttt tttgcttagc tgtagctaat tttttccgtc tcagcctgaa
    15961 gacttgtgat atgtccatct ttaagaagct ggtcaatttg ctcattccac ttggctgctt
    16021 ggtgttcttg ataatctctg cttgcaaagt caacgattcc tcctcccccg attaatctct
    16081 ggtagcagac tccttgcaga acaactgcca attcttcttc actcacaccc aactccagca
    16141 gacgctcata aacacctcgg atacttttgg cgtgaatggt tgaaaagact gtcgcacctg
    16201 tcaaactagc tctgaccact gcacgcgccg tctcgctgtc acgaatttct ccgataatca
    16261 agagatctgg tcgatgacgc aaggaaagtt tgattagatt ttcataggtt aggccgattg
    16321 cttcgttcaa ctgcaactga agcatgtcgt cctgcttgat ttcgacagga tcttcgatgg
    16381 acataacttg ctgtccttta aagagtgact tggacaattc atgcatcaag gtcgtcttac
    16441 cactcccaac cggaccagca aaaagataga gtccccgttg cctgtactgc ttgcctaatt
    16501 cttcaatatc ctgaaaccaa aaatgcaagt cctgctcctc atcgtgcaac aaacggataa
    16561 ccaaactctc atgcccccga taatcgccta cagtagataa acgtagagac gccatcttct
    16621 ggtcataggc ataatcacag gaacccagtt gactacgtct tttttctccc acattcatac
    16681 ccgccacaaa cttaaagtga ctgataacgg ctgcaaactt ttcaaaatca tagctaccaa
    16741 ttttacagcg ctcgtctcct accctcatat gaagctcata ggcatctaac ttagggacaa
    16801 aataaatgtc ttgcgcccct tttttccgag ccgaacgaat gatttcttgt gcaatttctt
    16861 gaaccatact tacctcctca cctatactat tcgcaaagcg ttagaaaaat aaaaaaagca
    16921 actccaaaaa agttactttt ctctatttta atttcatacg gcaagaacga ttcctttcct
    16981 gactggtttg tttttcatag cctgagcgta gtttttcgtc tgggataacc tgctcatcct
    17041 gcaaaagagc caaagaatgg tattgttgta aatactcatc acattcatca caccagcgtt
    17101 ggtcttccgg gtcccaaaaa atggtcatca agtcgcttct acttttataa agaggggatt
    17161 cttgttctaa tctaaaccag caagttttgt agtatttgag agcatcataa tactggtcaa
    17221 attttctact tgctacaata tcttcttccc aaccttctaa gaaccaccac ggttcaaaat
    17281 ctccgtacat ttctataaca cgatacatat tcattcattc ctttgtaccg tatattataa
    17341 ccaattccgc cagacaagga aagaaatatg ctcatttctt aattttttga taaaaaatcc
    17401 agccatctga tggatgactg aatttctatt tgcttatgta caaaaatatt tttactactt
    17461 aggcttctga gatatcgttt tcgataatga ccttaatggc atggtggtct gctgccttac
    17521 tgaagacttc gtaggctttt tcaatttcac tgagtttgaa atagtgagtt accaattttt
    17581 ccggttcaat cttatgactt tcaagtgctt tcaacaattg tggagtcgta tttgtagata
    17641 ccaaaccagt tgttacattg atgttacgaa tccaaagttt atctaaatcg aattcaactg
    17701 gtttaccatg cacaccacag ttggcaaccg ttccgtctac accgataatc ttttgacaga
    17761 aatcaaatgt ttcaggaatc cctacagctt caatagcgac atctacaccg cgaccatctg
    17821 tcaaatcata aatttctttt atcgcttttt cagggtctga agagtttacc ttatgagtcg
    17881 caccgaatga tagggcagtt tccaaacggt tatcgtctag gtctaccata atcaatttag
    17941 ctggtgaata gaattgggct gttaaaagag cagccaatcc aactggacct gaaccaataa
    18001 tggctacgct gcaaccaggt tctactttcc cttttaagac accaatttca tatccagtag
    18061 gcagaatgtc tgacagcata accaaagctt catctgacaa gtcttctgga gtatggtaaa
    18121 gagtattatc tgcatgaggg acacgtagat attcagcctg cataccatca atcaagtgac
    18181 cgaaaatcca gcccccttcg tcttcacagt gggcataaat tcctttttta cagtagtagc
    18241 atttaccaca ggcacagacg caagaaatca agaccttgtc accttttttg aagttggaaa
    18301 ctccttcccc aacttcttca acaatcccaa tcccttcgtg gccaagaatg gtaccacttt
    18361 ggcaagtagg aacatcccct ttgataatat ggaggtctgt tccacaaata gtggttttta
    18421 caatacgcac aatagcgtct gttggcttgc gaataactgg tttgtctaca tcaacaaaag
    18481 aagcaagtcc tggtttaaca taagtataag ctttcataaa gcactcctcc gtttttttat
    18541 ttgtaatatt tatagttcaa ttgtaagcct ttttctttgt ttcttcaagc tttttttgtg
    18601 atttttttaa ctttttattt accagtaaag taccctgcat aaaaaagcag aagttagtct
    18661 ctaacttctg ctttctctct tatgcttgat aacgtttcac gccatctagg taggttgcta
    18721 ccaattccaa atctttatct aatacgatga agtcagcgtc gtagccttca cggatttggc
    18781 cacagacgtc atcgatgtga acggatttag ctgggttgaa gctggccatc atgactgctt
    18841 catgcggatt cgcaattccc cattcgacca cattcttcaa accatctttg agtttgagga
    18901 tagaacctgc caagttacct gtcgatttga ggcgtgcagt tccatttgca acgacaactg
    18961 ggaattctcc caacatataa tctccgtctt ccaatccccc agctgtcata cagtctgtga
    19021 taagagcgat gttttctgtt cctttttgtt tgataagaat ttcgcaagcc tttggatcta
    19081 cgtggtgacc atcacagatc aactctgcat aggtatgtgg caattggtac atggctccaa
    19141 ccatacccaa ttcacggtga gtcaacccac gcattccatt gtaggcatgc acccaaacac
    19201 tcgctccagc atcgactgct tttttggctt catcaaaagt cgcgtttgaa tgtccaagag
    19261 caaccgtcac accttcgccc gtaactgtac gaacaaagtc ttccacccca tcacgttctg
    19321 gtgcaagggc aattttatta agcaagccat ttgccgcttt ttgccaagaa tgaaactcct
    19381 caacacccgg gtctctcata taagttggat tttgtgcccc cttaaaagtt tctgtgaaat
    19441 atggaccttc ataataaatc ccacgaatct tagcacctgt tgcttcttta taatggtttc
    19501 caagattttc agtgactgca agcaattgct cataagtggc tgttaaagtt gtgggtaaga
    19561 aactggtaac accggtacta agaagtcctt cactcatagt atgcaatgta ccttcaatgt
    19621 tgttgtccat cacatctaca cctgcatatc catgaatatg agtatccaca agacctgggg
    19681 caatgctata acctgtatag tcaatcacct cagccccttc aggaatctgc tctacatgtt
    19741 tcccaaactt gccgtccaca agttccaagt aaccacctcg acgaactccg tgtgggtaga
    19801 aaaactgatc cgctttaata tagttaggca taatgttaac ctccttaaaa gattgattct
    19861 acaatttatt atgtcaatta cattataaac ctttcttttt tatttgtaaa tggtatagac
    19921 ctattttttg gagatatttt aaaagagctg gatttctcca actcttctct tttttaatac
    19981 aagttatttt gatttaaccg gcttgtcttg agctgtctgc aaagctgtgg caatcgtatc
    20041 tgcatacaat tttgctcctg cttcgatagt gctactctca ctcccgaaat gaacctggtc
    20101 tgttcctgcc caaatttctg gatgctcttt cgcaacttga ttccaatctg ctatcgtaat
    20161 gtaaggtgtc ttctctgcca attctctcat ataggcagca gccttctcaa cgatggcata
    20221 ggtctctttt gtcttatctc cctcataagg agtcaccaaa atcatatggt gtcccttagg
    20281 aagatttttc acgatactgt cccagtcatc cttgtaattc tcaggattat ttaccccagt
    20341 cgcaatgacc accgtcttag gtaaaaattt attctggcta ttatttagca tgatttcatt
    20401 tgcggtcttg gttgttacgc tgacctgcgc gttaatctgt gctccaggaa gaactgtctg
    20461 tagtgctgta tttgccctta aagccactga gtcaccaatt aacatagtgc catcagcaat
    20521 tcccaaactg tttgcatctg cccgttctgc catcaccttg gtctggccaa tatttgttgc
    20581 agcttgcttc aagccattga cagtcaagtc tgtctcaaac gctcccactt gtggtgccaa
    20641 caaggtcacc atgcagacaa tgaaggtcaa gattcctgta cctgctgcaa gaattgcgtg
    20701 aatataaggc aggggacgaa gggtttggac aataggtgtg ttcttgcctg caatccaagg
    20761 ttccaataca tagaatgaca gactggcaaa gccataagaa caaatcagag tcagtaatac
    20821 agcaagaaga tttgatgtca actgtgagaa aatgatatag aaaggccaat ggaaaagata
    20881 aaccgcatag ctagtatccg ctaaaaagct gataatcttc ggctcctgta tatgatgtgt
    20941 cttttcatgt aagacacgcg ccgccagaat catggcaaga gctgcaagac tggcaagtaa
    21001 gaagccgata agataggcaa aaagataagt gaatttgaca aagaaagtca aaagaactag
    21061 gaaaccaaag cctcctccaa aaactaccaa agtctttcgt aaatcccaga ttttatccaa
    21121 ctgcttgacg agggaagtcg tctgacgaac gcctacaata gttgctaaca tacttcccaa
    21181 aaagaatgga tagacatgag ttaaactgga gaaataaaca gaggaataag aggtcactag
    21241 aaaactacca ataaacatgg agaagaaact gatcaagaag gcaacagcag ataagagaaa
    21301 gaccatcccc ttcaactgac catttgattt agcttgtttg gataagaacc aaactgccaa
    21361 tccccaaaga atatagtagt gaacctcaac tgccaaactc caattatgaa caaacaaatg
    21421 aggaatgaac tgagattcat aactcccacc tgttaggagt tcatagaagt tggtcataaa
    21481 gcctaagacg ctcgcaatct ggccaccaat tccagcaaca tagtcttggc gaaccaagaa
    21541 agtaaaaggc atggtcacca agaccatcaa aaccacaggt ggcacaatcc gataaaagcg
    21601 tcttctaaaa aatcctatca aatcaatctc atggttttta gaaaattctt caatgagtag
    21661 agctgtaatc aggaaacctg aaaatgtgaa aaagacatct accccgaaaa atcctccagg
    21721 aaagatcgtc tgaaagaagt gatacaagag taccaaaagt aaacctgtaa tcctaaccaa
    21781 ggaaaaccat ttaatgcgca tacgagttta ttcttccttt cattgtacac tttattctat
    21841 caaaaaaaga agaaaaatcc taagagaaaa cttaggattt ttagcttata tcaattccat
    21901 tttagaaatt acgtcctgac ttattatagc catatttttc cacaaaatac tcacggaatt
    21961 ccaagagatt gtcatccatg atggcttgtc gcacttgctt catcaagtta agcaagaagt
    22021 aaagattgtg gtagctagtc aagcggatac caaaggtttc atcagccttg agcaggtgac
    22081 gaaggtaagc gcgtgtatag ttattacatg tgtaacaatc acactcagga tccagtggcg
    22141 taaagtcctc agcaaactgg gcatttttca caaccaaacg tccttgactg gtcatacaag
    22201 tcccgttacg agcaattcga gtcggtaaga cacagtcaaa catatccacc ccacgaatga
    22261 ccccatcgat caagctatct ggcgctccca cacccatcag ataacgaggt ttattttcag
    22321 gtagcagttg agttgtaaag tccaagaccg cattcatctc ttcatgggtt tctcccactg
    22381 ccaaaccacc gatagagtag cctgagaaat ccatgctgac aagatcatga gctgattggc
    22441 ggcgaaggtc ttcaaatcct gcaccttgca caattccaaa caaaccttgg tcatgtggac
    22501 gacggtgagc cttcaaacca cgctcagccc aacggctggt acgctcgatc gatttcttaa
    22561 cgtagtcata aggttgataa aactgaggac attcatcaaa ggacatcatg atatctgaac
    22621 ccagattatt ctgaatagag atggcttttt ctggggatag gaacatctta gaaccattta
    22681 gatgattttt aaaggttact ccttcttctg tgatattacg gctatctgct aaagaataaa
    22741 cctgaaaacc accactatct gtcaagatag gctggtccca attcatgaac ttgtggagac
    22801 caccagcgcg tgcaatgagt tcatctccag ggcgaagcca gagatgatag gtgtttgata
    22861 ggataattcc cgaacccatc tccttcaatt cttcaggtga ctgagttttg acagtggctt
    22921 gtgtcccaac tggcataaac ataggtgtcg gaaaggtacc gtggggagtg atgatttctc
    22981 ctagacgagc tcctgtgtgt ttttctttct taatcaaacg atatttgatt ggtgaatctg
    23041 acatttttta cctccgaagc tgggaaagac agtcccagtt catacagtgt gcccaaggac
    23101 acactgtatg attttatcaa aaaaagtagg aactgtcacg aactatgtat aagaactatg
    23161 gtataataag agaatgaact atccaaaaat tgatttaaaa acaattcgtc aggagtcaaa
    23221 acattttcag gctgacaccc cccgtctctt tctcctctat atccttccca gtatgctggt
    23281 catcttatct agttttctta atcccttatc tcggatccac gggactgttt tagaacagcc
    23341 atttttcagc atacttggcc aaatacttca agcctacctc tttcctctac tagtctcctt
    23401 tatcgggact attcttttga ccagttccgt ctatgcaaca ctgacactca tgaaagattc
    23461 taaaacggag ccatctgtaa aaaacagcct agctctcttt gatgaggagc gcttttctca
    23521 aacctttttg accctacttc tcaaacgttt ctatctcttt ttatggagta tccctaattt
    23581 acttgggatt tacctcctct tttatagtag ctttttagcc aaaaaattcg tcaccctaca
    23641 tcctgagttt ccaaatctag atctctcatc agttgaaacc gagcgtttcc tcatggtctt
    23701 tggactctac tttcttgcaa gtctcatctt gatcattgta gggaatatcc tctatattcc
    23761 acagtattat gcctattcac aggtggaatt tcttctctgt tatagtctag acctaggaca
    23821 ggtcccacca agacgaatcc taaaaactag ccgttccttt atgaagggct acaagttcca
    23881 gcactttgtc ctagatttac agcttcttcc ttggtacttc ctcaattgga ttacctttgg
    23941 cattgctagt ttttcactcc taccttatat ccaatgcact aaaattatgt tttaccgagc
    24001 agtattggct cgaaaacgtc caaaagcttg aaggttttcc cctcaagctt ttttctatca
    24061 atgaatttct ccgcctacca acttgagttc ctgatctcca tgttctatca tagcgccgat
    24121 tgctgcttct atccctcgcc gaatatccac taaactcata gttggagtag tcggtctgtt
    24181 caccacctgt tccatcatat aaggaatatg cataaaacct gccttaacat atggaaattt
    24241 cttttctacc aaatagagag cctgatacat caaatggctg cagacaaaag tccctgccgt
    24301 attggaaaca gaggccggta atccttcttt tttatagctt gaaccatcgc tttaatcggc
    24361 aaactactaa agtaggccga agcaccatct gggcgaatgg gacggtcaat cggttgatta
    24421 tcttcgttat cagaaatgcg tgcatcgtct tgattaatgg ccactcgttc aggtgtcaaa
    24481 ctagttcttc caccagcttg cccaatacaa aggacaaagt caggttgata acgattcatc
    24541 tcttcttcca atacttgagc agatttgtga aaaactgtag gcacctctag ccaacggacc
    24601 tcagcaccat ggatttcagc tggtaaacct ttaatggcct ccaaagctgg attgcccttt
    24661 tcccctccaa agggctcaaa acctgtcact aatactttca tttctaactc ctcaaaacca
    24721 attcaatgag acttttttct taaaaacaag tataacatat ttcccttatt ctggattgaa
    24781 aaggattagc gagattagaa tcgaccttca agtgtattta aagagtaaaa tactaatcaa
    24841 ggccaaaata gctgatccac cttgcttcaa aataattttt ttatccgctg ttaaagagcc
    24901 gtaagtcgca gcaccaatca caaataagac aaaaatagtc acaatttcta aattctgtga
    24961 gaaataaatg ccatacaaga gaaagactcc tagcagagcc ttataaatcc cttgattttt
    25021 gaacaataaa cttactgacg gatgagccaa ttcttccttt tccatattaa atacacgact
    25081 agtcgcatct gattgcgtgg caatactttc caaataaaaa atgtaaaaat gctccaaagc
    25141 aacgatcgtt gttaaaataa ttgtaataat tgacatctat ttctccttaa atttctatat
    25201 tttcaatacc caaaaccaat ttatttaata aacggctgag ttctgttctc tcaaactctg
    25261 ttaagatact ttccatctct tccttaacct tgatatgttg cagaggggga ttcaccacta
    25321 actgctcctt ggcatacttc gcagcctcta ccaacacttc ccgctgattt tcaggattac
    25381 gatgacgctc caccaaacct tccgtttcca aaatcttgaa atgccgtgtc aaagcagcct
    25441 gatcaatttt caaacgctcc tgaaccgcca tttggttaca aggagaatgc tccagcaaaa
    25501 acagtaaaat ctgataccgt gtcaaactaa tcccaagcct tttttcaaac aattgcgtac
    25561 tcgcttgctc agacaagcgg agttgataca gtaaatcttg aatctctagc atttcttccc
    25621 ctctcttttt attgatttat caatatttga ccagtcaatt ataatataaa gtatagtgaa
    25681 atgaaataag agcaggacaa atcgatcagg acagtcaaat cgatttctaa caatgtttta
    25741 gaagcagttg tgtactattc tagtttcaat ctactataaa atagattgca agaatgttgc
    25801 ttaactcatt acaagatgat aactttccat tttccattca aattgatact caatgaaaat
    25861 caaaaagcaa actaggaagc tagtcgcagg ttgctcaaag cacagctttg aggtttcaga
    25921 taaaactgac gttgtttgaa gagattttcg aagagtatga caaagaaaaa acgaacgaga
    25981 atcgcttcct atcacgaaag ctagatctca ttcgtcaaaa tgatgttacc aagtcgggtt
    26041 tatccattca ttgaaacgtg aacgtggtca tagtgatttt ctgtcacact accacggtct
    26101 ggcattgggt tccaagtgtt agctggccca tatttgctat cgaatggagc atagaaacgt
    26161 tgtttccaga tgatgtaact aatgccacgg ctggccatgt tttgaatagc atattccgca
    26221 atcttatccc ctaattctga actttctggt accataaagt cgatagccaa accttttccg
    26281 tgatctccac tgtctcctgg acgataacca ctaaaggatg taatgccaaa caagttagca
    26341 atttcttctt taaaggcagc tgtttgtggt tgaagacctg cattttcaga tttcgctact
    26401 gccagtccag cataatcggg cgcagctgga gcctcgtaag ttgttgaaat tgttttcgtc
    26461 tcttctggtt gataagtaga aactgctgct gtagctccat ttgatgatgc tacttctttt
    26521 gcttctgaac ttgttgcagt tgtttcggtt gctttttctt ctactggcat tgtagttgct
    26581 tccactgctg actcggtttg agagctcact tgtgtttcct gcttctcagc tagagtcgtt
    26641 gcctgaggag ctacttcttc aactggacta gttgtttcgg ctggttgtcc ctctgggaca
    26701 gaagtgcccg tagatggtgc cacttcttca gaagcaatca ctgtctttgt aacttctgaa
    26761 cttgatgcta cttcttttgg agcttctgca attggttgag aaaggtctgc aacctgaaca
    26821 gtttgatcat caacggtcac ttgattagtg gtcaaatctg ctgtcgcagt tgtcacttct
    26881 tcactagagt ctgcttgagg tgtttggatt tcaacttctg ttacttcttc tgcttcattg
    26941 acagtcgttg tcaaaacagt ttctgggaaa atcaagtcca tattagtgat tttgttcaga
    27001 ttcgcaagca ctgtgacatc tacacccaag gcttctgcaa tagtgctcaa ggtatcacca
    27061 tactgtacgg tataacttgt tttgttgtcc gttttagtca aatcgttttg gatttgctca
    27121 acactacgtg cagtccaaag aacttcttct gcttgagttg ccaatactgg ggcaaatgac
    27181 aaggctactg ttgacgctaa taacattctt ttcttcattc tttcaaattc ctttcaaatt
    27241 agtacctgtc tatcataaca caaaaaatgt agaaaaaaag ccctctcggg cctatttaac
    27301 agaactgtct attccttgta acaaactagg ttacttgaaa tagtttgtta ggtctctgtc
    27361 acaaaactga cacaatcttt ttatcactta gtctcaaaaa tacgggaaat atctgctaac
    27421 gcttgaatcc tgtgattccc ttcataagta gactctaaaa agttgatact ttgaatccca
    27481 ctattctggg caaattccac atccagagtc cgatccccta tataataagt attatcagaa
    27541 ttcaactgat acttatctag cagataggta gccgcttctg gacttggctt ccgcacaaag
    27601 ccactctgac tggttaaaat ctctgtaaaa taggattcca cccccaagtc cttgagaatg
    27661 gtaaaagcgt tgttcccctt atgagtatat ataaactgct gaattcctga ttcgtctgcc
    27721 caagctagca cctcacgcgc acctggcatc aaaactacct gagcattttt ctcagccaga
    27781 ctctgggcac gcacctgatt tagcacctca acatccagat ttctatcttc tgccacccgc
    27841 acaagcaaat tttgcaccga atacttgaag ataaactctc tcaccttctc cttatcataa
    27901 ggaatagaaa actgagcaaa agtctcctca atccctgata aaatcgcttc gtaagagtcc
    27961 aataaagtcc cgtctaaatc ccaaataaaa gctgtttttt gcatttccta tcctttcaag
    28021 ctcatatagc gctggtaacg atagcgtaga aataaccagc gaaaaccatt atccaagaga
    28081 gaccctgccc aaataccagg caagccccaa ccaagcacaa tccccatcag atatcctgtc
    28141 ccaatgcgga tacaccacat gcctatactt gtcgcataaa aagggaggcg tgcatttcct
    28201 aatccctgcc agactgccgt atagatgact gttcctgtcg tcataggggt cccaagtagt
    28261 gaaaacagtg tcactagaac actagcctcc accgctagag aatcagtcgt atagagatga
    28321 gttaatggta cacccaagac atatatacta aaggacaggg gcaacatgag gaacagagaa
    28381 agccaaaagg tttgtttact caaactagca actcttttcc aatcatcctc tccaactgct
    28441 cgggccaaca gcatgaccgt tgccgtagcg acgccaaagg caggcatata gttaaactgg
    28501 gtcaagactt ctccgattgc attcccagca actgcctccg tcccaaaaga aacgaccaag
    28561 gcaatgatca ctacatctcc agccctcatc ataagtcgct ctccagctgc tggtaaagcc
    28621 aaggtcaaca gttccttatc taaaccaaaa gttggcttcc cataaggcag ttttaattgt
    28681 gaccacaaaa tcacaagacc aaccaaacga gacagaattg tcccccaagc aacaccagct
    28741 atccccatat ccagaacaaa aatagctaga cttgaaaaaa gaatattcaa ggcattggat
    28801 aaaaaactaa catagagagg cagacgtgga ttatgcgttg cacgaatcaa ggctcccaga
    28861 ctagtcatta aacctaagag aacaatcgat ccgcctacca aagatagata cagtccacca
    28921 ctctcagcta catccctctc cgtccccaaa agtcctatca tctctttccc agcgaagatg
    28981 gacaaaaatc ctaaaaggaa acttaatagt aaggtaatct tcaacgcctc agtcacatga
    29041 taggccaact tcgactggtc tttctgccct atgctttttg aaataacact ggaaatagca
    29101 gctcccagag cgatgaaaat cgcctgataa atggtgataa tattaccagc tactgaaacc
    29161 cctgaaatag ctatcaatcc taaatgagca accaaataac tgtccaccat tcccattagc
    29221 atctgcaaaa agttttcacc catagctggc aatgcaatat taagaatgtc tttatttttc
    29281 ttaaacaatc tctcctcctg atgaaaagaa actcagttgg tttcccaacc gagtttactc
    29341 cctctatctt aaagtcctaa ataagcctca accgctgctt gcatatcagc agccgctaca
    29401 gttgtcttgt gacgaacagg agctgtctca agcccatcaa ctgctggtgg cactgcaacg
    29461 cctgagattt catgtaattg agccaaggct tcaaagtctg ttaaacctgc ttttccagtt
    29521 acagcttcta ctgcaactac tgggaacttg tagggactag ctgttgaagc aatcactgtc
    29581 ttagtcgcat catcagtaac cgcttggtat tttctataaa ctgctgaggc aaccgccgta
    29641 tgtggatcct caatataaga atctaactca taaacacgct tgatttctgc cgctgtttct
    29701 tcctcagtcg catattcagc tgcaaagagc tccagaatct ctgcatcaaa gtctgtcaac
    29761 ttatattgtc cttgcatgtt caaagcattc atgagttcag ctgtcttttc agcattattt
    29821 cccaaaagat ggaaaatcaa gcgctccaag tttgaagata ccaagatatc catagatggg
    29881 ctggttgtta ccttaaactc acgttttttg tcatagacac gtgttttaaa gaagtctgtc
    29941 aaaacattgt tgtcatttga agcacagatc aatttaccaa ctgggagacc gatttgtttg
    30001 gcataaaagg cagccaagat atttccaaag tttcctgttg gtactgtgaa gttaatctta
    30061 tcaccagcca cgatctcacc agtcttgacc aactgagcat aggcataaac ataataaaca
    30121 atctgtggca ccaaacgacc gatattcata gagttagcag atgaaaattg caacttgttg
    30181 gccgctaatc tttcacgaag agccacgtcg ttaaacatgt gcttcacgtt ggtttgcgca
    30241 tcgtcaaagt taccatctat agcgataaca tgagtattgt caccattctg agtggtcatt
    30301 tgcaactctt gtaccttgct gacaccaccc tttggataaa agacgataat ctcagtacca
    30361 ggcacatccg caaaccccgc catagcagct ttccccgtgt caccagatgt cgctgtcaag
    30421 ataacaatct tgttctccaa accatgtttc ttagcagcag tcgtcataaa gtatggcaaa
    30481 atagacaagg ccatatcctt aaaggcaatc gttgaaccat ggaaaagttc caaattgtat
    30541 tgcccatcta atttcactaa tggtgcaata gctggagtat caaatttgct atcgtaggca
    30601 ttgttgatac agtagtccaa ctcctcagct gtaaagtcat ctaaaaatgc tgacaaaact
    30661 agcttagcaa cttcctggta agaagcatct ttcaatttgt caaagtccaa atctaccttt
    30721 ggataagtaa ccggtgtaaa caaaccgccg tccgtcgcca aaccttgcaa aattgcttgg
    30781 ctggcagtta ctgtattgtt ggcatcacgc gttgattgat aaactaatgt cattactctc
    30841 tatcctttat ctatagtctc gtccattata tcatgatttt tcagaaaatt ctccctttat
    30901 aagagaaatt ataggctcaa ttcttctctc aaaggctgta aataggtcat ttcttcctta
    30961 tttctcacca ctagtccctc ctcagctagt aggagtaagt ctttggaaaa ttcaataatc
    31021 gtagtttctt cctcatctgt aagatttttc ttagaaaatt gtctccttaa agacttgtaa
    31081 tcataaccaa atactttaaa gaaaggtgct gtttctaagt aagcttctaa cttgtctaaa
    31141 ttaaccaata atcccaagtg aaaagctgca gaagcaaaag tcctatcaag tggctgtgta
    31201 cacacactac gaaactcaac tgttcctcga gtcgttaagt cttggtactg gtaactacga
    31261 tgagtttcaa aatccttctc ttgggggtaa ataataacct catccccatt cagagcaaat
    31321 gcttggattt caggcgtagc caaatagtcc ccagcctgaa taggataaaa ataataggtc
    31381 tgcccatcac gttccgcagt aaaaatcgca gaatgattta gatagtcaaa aaaatcagct
    31441 tcatcattaa ggagtctagc attgacccca acattctctg gatagatacc atgcatagat
    31501 tcttcccaga aaatatccct tgaaattttc gtatcccaat ccgcacccga aaattcagag
    31561 tttgcaaata aataagcctt agccgcttca atttgagtaa aagcattaat cacccgtaag
    31621 tagttggttt ttgaaatatc cagctgaacc tggctcccac agataaaagt accatattca
    31681 gggaaatgat gtaaatctga tttaataata tttctactca aattcaaata atccatcaac
    31741 atctgatagc gtggataagc cactggacaa ttctcatttt tatcccagtt gggatggata
    31801 ccacagccaa caatagcatg atttgattca gctaactttc tctgaattac attcatataa
    31861 ttattgaaac gattttcgac ctcttgaatc gtttcagcct taccaaatgc aaactcaatc
    31921 gttgtatagg aaacttcaaa taaaatagca tcctgactta tcggatctac taactggatc
    31981 agattgccaa aatcatctac ctttgctacg gtgagatcca aagtagaaac taaataatga
    32041 aatagatcct ttataacttc aacatctgta gcatcccctt ctaaacttgc aacaggatac
    32101 tccaactcaa ttccgacaaa caattcagga ttttctttaa tatttttcaa ataccgatgt
    32161 tttaataaat cgatagaaca agacatacta ccctacactt tctcaatcta aacaaaattt
    32221 gaataactat aattatacca aaaataatat aaaaaaggaa cgaagaataa caacgttcct
    32281 aacactatac tataccggcg gccggggtcg aaccggcacg tccttgcgga cactggattt
    32341 tgagtccagc gcgtctgcca attccgccac gccggcaaat agtaactggg gtagctggat
    32401 tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta taccccaata
    32461 taaaataggc gagtgatggg gatcgaaccc acgcatgcca gagccacaat ctggtgtgtt
    32521 aaccacttca ccacacccgc cataattcta ttaacacggg cagtaggaat tgaacccaca
    32581 ctgaaggttt tggagacctt agttctacct ttaaactatg cccgtaaaat ggaaggggag
    32641 ggattcgaac ccccgaaccc gaaggagcgg atttacagtc cgccgcgttt agcctcttcg
    32701 ctacccttcc aaaatatata aatggcgcga gacggaatcg aaccgccgac acatggagct
    32761 tcaatccatt gctctaccaa ctgagctacc gagccttatt gcgggagcag gatttgaacc
    32821 tacgaccttc gggttatgag cccgacgagc taccgagctg ctccatcccg cgttaatata
    32881 aaaggaggat gtgggattcg aacccacgca cgcttttaca cgcctgacgg ttttcaagac
    32941 cgttcccttc agccggactt gggtaatcct ccaaaatagt ttatacggga acaagcccgt
    33001 gtcctcttag tgaacagata atattccaat cccctagtaa tggactaaac actatgggca
    33061 cgagtggact cgaaccaccg acctcacgct tatcaggcgt gcgctctaac cacctgagct
    33121 acgcgcccaa gtcaaaaact tggtacaaag aacaaagttc aaagcgggtg acgagaatcg
    33181 aactcgcgac aacagcttgg aaggctgtag ttttaccact aaactacacc cgctaaaatg
    33241 ggagttaacg g
//
LOCUS       DAVKQB010000022        33230 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKQB010000022 DAVKQB010000000
VERSION     DAVKQB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.022"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            161..2794
                     /gene="polA"
                     /locus_tag="VW528_001028"
     CDS             161..2794
                     /gene="polA"
                     /locus_tag="VW528_001028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9911859.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVGTEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2879..3316
                     /locus_tag="VW528_001029"
     CDS             2879..3316
                     /locus_tag="VW528_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9911860.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(3552..4562)
                     /locus_tag="VW528_001030"
     CDS             complement(3552..4562)
                     /locus_tag="VW528_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU9911861.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSIVLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            4711..5880
                     /locus_tag="VW528_001031"
     CDS             4711..5880
                     /locus_tag="VW528_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9911862.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEVFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            5877..6647
                     /gene="recO"
                     /locus_tag="VW528_001032"
     CDS             5877..6647
                     /gene="recO"
                     /locus_tag="VW528_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9911863.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKCMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            6644..7636
                     /gene="plsX"
                     /locus_tag="VW528_001033"
     CDS             6644..7636
                     /gene="plsX"
                     /locus_tag="VW528_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9911864.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            7642..7875
                     /locus_tag="VW528_001034"
     CDS             7642..7875
                     /locus_tag="VW528_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9911865.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            7921..8211
                     /locus_tag="VW528_001035"
                     /pseudo
     CDS             7921..8211
                     /locus_tag="VW528_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8414..8644
                     /gene="blpU"
                     /locus_tag="VW528_001036"
     CDS             8414..8644
                     /gene="blpU"
                     /locus_tag="VW528_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU9911866.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            9359..11512
                     /locus_tag="VW528_001037"
     CDS             9359..11512
                     /locus_tag="VW528_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9911867.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            11525..12874
                     /locus_tag="VW528_001038"
     CDS             11525..12874
                     /locus_tag="VW528_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9911868.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKVTASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKYVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            13044..13751
                     /locus_tag="VW528_001039"
     CDS             13044..13751
                     /locus_tag="VW528_001039"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9911869.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNVAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            13753..13896
                     /locus_tag="VW528_001040"
     CDS             13753..13896
                     /locus_tag="VW528_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9911870.1"
                     /translation="MFATENLRLSTKRTQAEKVPQTFSLCRELGELTDVYEIVWEKLQ
                     GLK"
     gene            13953..17678
                     /locus_tag="VW528_001041"
     CDS             13953..17678
                     /locus_tag="VW528_001041"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9911871.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            17771..19213
                     /locus_tag="VW528_001042"
     CDS             17771..19213
                     /locus_tag="VW528_001042"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9911872.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGVEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            19432..20454
                     /locus_tag="VW528_001043"
     CDS             19432..20454
                     /locus_tag="VW528_001043"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9911873.1"
                     /translation="MANKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIAGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            20451..20996
                     /gene="purN"
                     /locus_tag="VW528_001044"
     CDS             20451..20996
                     /gene="purN"
                     /locus_tag="VW528_001044"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9911874.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            21080..21589
                     /locus_tag="VW528_001045"
     CDS             21080..21589
                     /locus_tag="VW528_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9911875.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            21614..23161
                     /gene="purH"
                     /locus_tag="VW528_001046"
     CDS             21614..23161
                     /gene="purH"
                     /locus_tag="VW528_001046"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9911876.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            23283..24545
                     /gene="purD"
                     /locus_tag="VW528_001047"
     CDS             23283..24545
                     /gene="purD"
                     /locus_tag="VW528_001047"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9911877.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            24948..25436
                     /gene="purE"
                     /locus_tag="VW528_001048"
     CDS             24948..25436
                     /gene="purE"
                     /locus_tag="VW528_001048"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9911878.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            25423..26514
                     /gene="purK"
                     /locus_tag="VW528_001049"
     CDS             25423..26514
                     /gene="purK"
                     /locus_tag="VW528_001049"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9911879.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            26524..26751
                     /locus_tag="VW528_001050"
     CDS             26524..26751
                     /locus_tag="VW528_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU9911880.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            26814..28112
                     /gene="purB"
                     /locus_tag="VW528_001051"
     CDS             26814..28112
                     /gene="purB"
                     /locus_tag="VW528_001051"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9911881.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(28167..32105)
                     /gene="strH"
                     /locus_tag="VW528_001052"
     CDS             complement(28167..32105)
                     /gene="strH"
                     /locus_tag="VW528_001052"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9911882.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLETLDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(32420..33136)
                     /locus_tag="VW528_001053"
     CDS             complement(32420..33136)
                     /locus_tag="VW528_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9911883.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT         9233 a         6407 c         8028 g         9562 t
ORIGIN      
        1 gtgtactatt atagattcaa tatactatat tatatcacat ttttaaatct aggtttacta
       61 aattctctgg ctctatctat ttattcggaa aaagtttgaa aaatacttgc tctagctctt
      121 cccaatggta ttttttgatt ctttccttta taatgggtgt atggataaga aaaaattatt
      181 attgattgat gggtcttctg tagcttttcg ggcgtttttt gcgctgtatc agcagttgga
      241 ccgttttaag aatgcggctg gtttgcatac caatgcgatt tatggttttc agttgatgtt
      301 gagtcatttg ttggagcggg ttgagccgag tcatattttg gtggcttttg atgcgggaaa
      361 gacgaccttc cggacagaga tgtatgcgga ctataagggt ggtcgggcca agactcctga
      421 tgagtttcgt gagcaatttc ctttcattcg tgagttgctg gatcatatgg ggattcgtca
      481 ctatgagctg gctcagtatg aggcggatga catcattggg acgctggata agctagcaga
      541 gcaggatggt tttgatatta ccattgtcag tggggacaag gatttgattc agctgacgga
      601 tgagcatacg gtggttgaaa tttccaagaa aggtgtggct gagtttgagg cctttacgcc
      661 agattacctc atggaagaaa tgggcctcac accagctcag tttatcgatc tcaaggcgct
      721 catgggtgat aagtcggata atatccctgg ggtgaccaaa gtcggtgaaa agacgggtat
      781 taagctcttg ctggagcatg gttcgcttga ggggatttat gaaaatattg atggaatgaa
      841 gacttctaag atgaaggaaa atctcatcaa tgacaaggaa caggcctttt tgtctaaaac
      901 actagcgacc attgatacca aggcaccgat tgcgattggt ttagaggact tggtctatag
      961 tggtccagat gttgaaaatc ttgggaaatt ttacgatgag atgggcttca aacagctcaa
     1021 gcaggcttta aatgtgtcgt cagctgatgt ggctgagggt ttggatttta ctattgttga
     1081 ccaaatcagt caagatatgc tgagtgaaga gtctatcttc cactttgagc tttttggtga
     1141 gaattaccat acggataatt tggttggatt tgcctggtct tgtggggata agctctatgc
     1201 cacagacaag cttgagctgt tgcaagaccc gattttcaag gatttcttag aaaaaacatc
     1261 tctgagagtt tatgacttta agaaggttaa agttcttttg caacgttttg gtgtggattt
     1321 gcaggcgcct gcttttgaca tccgtttggc taaatacctc ctttcgactg tggaggacaa
     1381 tgaaattgcg accatcgcta gtctttatgg tcagacttac ttggttgatg atgaaacttt
     1441 ctacggtaag ggtgttaaaa aggccattcc tgaacgtgag aaattcttgg aacacttagc
     1501 ttgtaaactt gctgttttgg taggaacaga gcctatttta cttgaaaaac tcagcgaaaa
     1561 tgggcaatta gagcttcttt atgatatgga gcaacctctg gcttttgttc ttgccaagat
     1621 ggaaattgct gggattgtgg tcaagaaaga gaccttgctt gagatgcagg ctgaaaatga
     1681 gcttgtcatt gaaaaactga ctcaagagat ttacgagctg gctggtgagg agtttaatgt
     1741 caactcgcct aagcagttgg gcgtgcttct ctttgagaaa ttgggacttc ctctagaata
     1801 cactaagaaa accaagacag gttattcgac agcagtggat gtgttggagc gtctcgctcc
     1861 tattgctccg attgttaaga aaatcctgga ttaccgtcaa attgctaaga ttcaatctac
     1921 ttatgtaatt ggcttgcagg actggatttt ggctgatgga aagattcata ctcgctatat
     1981 gcaggatttg acccagaccg ggcgtttgtc tagtgtggat ccaaacttgc aaaatattcc
     2041 tgctcgattg gaacaagggc gcttgattcg gaaggctttt gtgccagagt gggaggatag
     2101 tgtgctactc agctctgact attcacagat tgaattgcgc gttttggcgc atatttctaa
     2161 ggatgagcac ttgattaagg ccttccaaga gggggcagat atccatactt cgacagccat
     2221 gcgggtcttt ggcattgagc gtcctgataa tgtgactgca aatgaccgtc gcaatgccaa
     2281 ggcagttaac tttggagtgg tttatgggat ttcagacttt ggcttgtcta ataatttggg
     2341 aattagtcgt aaggaagcca aagcctacat tgatacctac tttgaacgtt ttccaggtat
     2401 taaaaactac atggatgaag tggtgcggga ggcgcgtgat aagggctatg tagagaccct
     2461 ctttaagcgt cgccgtgagt tgccagatat caattcgcgc aacttcaata ttcgtggttt
     2521 tgcggagcga actgctatca actcacctat ccagggttcg gcagcagata ttctcaagat
     2581 tgccatgatt cagctggata aagccttggt tgcaggtggt tatcagacta agatgctgtt
     2641 acaagtgcac gatgaaatcg tccttgaagt gcctaaatct gaattggtag agatgaaaaa
     2701 attggtgaaa caaaccatgg aagaagccat tcaactcagt gttcctctta tcgcagatga
     2761 gaatgaaggg gcaacctggt acgaggctaa ataaaaaggg ggctagtcct ccttttttgt
     2821 agtagaattc tgcaaacctt ttcaaaatgt gctatactga tgaaaaagga ggatttctat
     2881 gagtcaagaa tttatcaatc caagtgatgg cgtgattcgt cagtatctcg caacgagtaa
     2941 aacccttgct gtggtgggtt tgtctgaccg tgaagaaaca actagcaatc gagtaacaaa
     3001 ggaaatgcag gctcggggct ataaaatcat cccagtcaat cccaaggcgg caggtggcga
     3061 aatcttgggt gaaaaggctt atgctagcct cgctgaaatt ccttttcctg tagatattgt
     3121 caatgtttat cgtcgcagtg aatttctgcc cgatgtggcg cgtgattttc tcaaagctga
     3181 tgccaagatt ttttgggcac agctaggact tgaaagttta gaagcgaaag agattttgcg
     3241 tgatggtgga tgtgatgata tcgtgatgaa tcgctgtatc aagagagaac atactcgctt
     3301 gattgaggaa gcataagaaa aaggtagctg atgggctacc ttttgtgtta tactcaatga
     3361 aaatcaaaga gcaaactagc aaactaggaa actagccgcg ggctgctcaa aacactgttt
     3421 tgaggttgta gatagaactg acgaagtcag ctcaaaacac cgttttgagg ttgcagatag
     3481 aactgacgaa gtcagtaaca tatatacggc aaggcgacgt tgacgtggtt tgaagagatt
     3541 ttcgaagagt attagaaaat gccgataagg gtctgcatac caagagtggt gaggatgatg
     3601 gcaatccagc agatggctcc gagaacaatg gattttccac tggatttgac catagcgacc
     3661 agattggttt tgagaccgat agcactcatg gccatgacaa tgaggaattt agagagttgt
     3721 ttgagagggg taaagaaact actagacaca ccgagagagg tcagaagggt ggttaggagc
     3781 gatgcaagga taaagtaaag gataaaaagt gggaagactt ttttcagttg taggctttgc
     3841 ttgttttctt gttggcgact ttgccagtag gatagaaaga gcgtgatagg gataatggcc
     3901 aaagtacgtg tgagtttaac aatggttgca gactcgaggg tattgctttg gtaaagactg
     3961 tcccaagcgc tggcggcagc cgttacagag gaagtgtcgt tgaccgcagt ccctgcaaag
     4021 agggcgaagc cttcattgga tagatgaagc caggtgccga gggttggaaa gatgagcgca
     4081 gccaagacat tgaagaaaaa gataacggaa atggcttggg ctacttcctt ttccttagca
     4141 tcaataacgg gcgctgtcgc tgcaatggca gaacccccac agatagaaga acctactcca
     4201 accaaggtag ccagttttgt atccagtgca aagaaacgct ggaagaggta ggcaataatc
     4261 agagctattg agatagtgga caggatgaca gggagtgaag attggccaac tgcgaagacc
     4321 tgcgagatat tgagaccaaa accaagcaag acaacggcat attggagcaa cttcttggaa
     4381 ctaaaggtca aaccagcatc cagttgttta taggacgaga gaaagggatg taggagcatg
     4441 cctatgaaaa tggcaaaaac gggcgcgcca atcacaggga agaatcctcc taagtaccaa
     4501 gataggatgg aaatgagaag gcaggccaag atacctgctc cattttttga tagaaatgac
     4561 ataaaaacct ccaaataaaa tctattacca ttatagacct ataaacagga aaagtaaaat
     4621 agaaagtgga aagctattct ctaatgtatt tttgcggtcg gggggctttt gtagtataat
     4681 agagatacgt tttgaaagta ggaggtatct atggacttaa ctaagcgctt taataaacag
     4741 ttagataaaa ttcaagtttc gttgattcgt cagtttgacc aggctatttc ggagattcct
     4801 ggggtcttgc gtttgacctt gggggaacct gattttacaa cgccagacca tgtcaaggag
     4861 gcggccaagc gagcgattga tcagaaccaa tcctactata cagggatgag tggtctgctg
     4921 actctacgtc aggcagccag tgactttgtt aaggaaaagt accaactgga ctatgctcct
     4981 gaaaatgaaa tcttggttac aattggggcg acagaggctt tatctgcgac tttgacggct
     5041 attttggaag agggagacaa ggtacttttg ccagctcctg cttatccagg ctatgaaccg
     5101 attgttaact tagttggggc agaaattgtt gagattgata cgactgaaaa tggttttgtc
     5161 ttgactcctg agatgttgga gaaggccatt ttggagcagg gtgataagct caaagcggtt
     5221 attctcaact atccagccaa tccgacagga attacctaca gtcgagagca gttagaggcc
     5281 ttggcagctg ttttacgcaa gtacgaagtt tttgttgtct gtgatgaggt ttactcagaa
     5341 ttgacctaca caggcgaagc ccatgtgtct ctaggaacga tgttgagaga ccaggctatt
     5401 attatcaatg gtttgtctaa atcgcatgcc atgacaggtt ggcgtttggg gctgattttc
     5461 gctccggcga ccttcacagc ccagttaatc aagagtcacc agtacttggt cactgccgca
     5521 aataccatgg cgcaacatgc tgcggtagaa gccttgacgg ctggtaaaaa tgatgcggag
     5581 cccatgaaga aggaatatat ccagcgtcgg gactatatca tcgaaaaaat gactgctctt
     5641 ggttttgaga ttatcaaacc agacggtgcc ttctatattt ttgctaaaat tccagcgggc
     5701 tacaatcaag actcctttgc ttttctgaag gattttgctc agaagaaggc cgttgccttt
     5761 atccctggtg cagcctttgg acgttacggg gaaggctacg tccgcctatc ttatgcagcc
     5821 agcatggaga ctatcaaaga agccatgaaa cgacttgagg agtacatgag agaagcatga
     5881 ttcagtctat cacgagtcaa ggcttggtgc tttacaatcg caattttcgt gaggatgaca
     5941 agctcgtcaa aatttttaca gagcaggttg gcaaatgcat gttttttgtc aaacacgctg
     6001 gtcagtctaa gctggcgcct gttattcagc ccttggtgct ggcacgattt ctcttgcgaa
     6061 tcaatgatga cggactcagt tacatcgaag actatcatga ggtcatgact tttcccaaga
     6121 ttaatagtga cctctttgtc atggcctatg cgacctatgt ggcagctctt gcagatgcta
     6181 gtttgcagga caatcagcag gatgctccct tgtttgcttt tttgcaaaag actttggagt
     6241 tgatggaagc aggcttggat tatcaggttt tgaccaatat ttttgaaatt caaattttga
     6301 ctcgatttgg aatcagcctc aattttaatg agtgtgtctt ctgccatcgg gttggtcagg
     6361 cttttgactt ttctttcaaa tatggagcct gcctctgtcc agagcattat catgaggata
     6421 agagacgttg tcatctcaat cccaatatcc cctatctgct caatcaattt caagctattg
     6481 attttgagac tttggagacc atttcgctca agcctggaat caagcaagag ctacgccaat
     6541 ttatggatca attatatgaa gagtacgttg ggattcacct aaaatcaaag aaatttattg
     6601 attccctagc agactgggga caattactaa aagaggaaaa gaaatgaaaa aaatcgcagt
     6661 agatgccatg gggggcgatt acgcacctca ggccattgtt gagggtgtca atcaagccct
     6721 atctgacttt tcagatatcg aggttcaact ttacggagat gaagctaaaa tcaagcaata
     6781 tctgacagcg acagagcgcg tcagcattat ccatacggat gagaagattg attcggatga
     6841 tgaacctacg agagctatta ggaataagaa aaatgccagt atggtattgg cagccaaggc
     6901 tgtcaaagat ggtgaagcag acgctgtcct ttcggctggg aatacaggtg ccttgttggc
     6961 agcaggattc ttcatcgtgg gtcgtatcaa gaatatcgac cgtcctggac tcatgtctac
     7021 cttgcctacc gttgatggaa aaggttttga catgctagac cttggtgcca atgcagaaaa
     7081 tacagcccag cacctccatc aatatgcggt tctaggttcc ttctatgcta aaaatgtccg
     7141 tggcattgcg caaccacgcg ttggtttgct caacaacgga acagagagta gcaagggcga
     7201 cccgcttcgt aaggaaactt atgaattact ggcggctgat gaaagtttga actttatcgg
     7261 aaacgtggaa gcgcgtgatt tgatgaatgg cgttgcagat gttgttgtgg cagatggttt
     7321 cacgggaaac gctgtgctca aatccatcga agggacagct atgggaatca tgggcttgct
     7381 caagacagct attacaggtg gtggtcttcg agcgaaacta ggtgccctcc ttctcaagga
     7441 cagcctcaga ggtttgaaaa aacagctcaa ttattcagat gttggtggag cggtcttgtt
     7501 tggtgttaag gcacctgttg tcaagactca tggctcaagc gatgccaagg ctgtttatag
     7561 tacgattcgc cagattcgta ccatgctaga aacagacgta gttgcccaga ctgcgcgtga
     7621 attttcagga gaataaaaga gatgacagaa aaagaaattt ttgaccgtat tgtgaccatt
     7681 atccaagagc gacagggaga ggactttgtc gtgacagaat ccttgagtct gaaagacgat
     7741 ttggatgcgg attctgttga cttgatggag tttatcttga ccctggaaga tgaatttagt
     7801 atcgaaatca gcgatgaaga aattgaccaa ctccaaaacg taggagatgt ggttaaaatc
     7861 attcaaggaa aatagcaatc ggagttccaa gtcaacggaa gtagatggtt tttagaaatg
     7921 agaaatatcg gacaagctgg taaaatcttg gctgacagtg gttatcaagg gctcatgaag
     7981 atatatcctc aagcacaaac tccacgtaaa tccagcaaac tcaagccgct aacagttgaa
     8041 gataaagcct gtaatcatgc gctatctaag gagagaagca aggttgagaa tatctttgcc
     8101 aaagtaaaaa cgtttaaaat gttttcaaca acctatcgaa atcatcgtaa acgcttcgga
     8161 ttacgaatga atttgattgc tggtattatc aatcatgaac taggattcta gttttgcagg
     8221 aagtctaata gtaaaaaagt gattagaaaa catctttttt aaaaatagag atgattttga
     8281 aacaaaaaag ctaattcaag acgtttcgat gccaattcaa gatttggatg aaaaaaatta
     8341 atagatactg ttatactaaa cttgtcaagt ttgtaacaag acaaatatta aaaataaaaa
     8401 agaggtattc gttatgaata caaaaacgat gtcacaattt gaaattatgg atactgagat
     8461 gcttgcttgc gttgaaggtg gcggatgcaa ttggggagat tttgccaaag caggtgttgg
     8521 aggaggagca gtacgaggtc ttcagctagg aattaaaaca agaacatggc aaggtgcagc
     8581 aactggtgct gcgggaggag ctatacttgg aggtgtggcc tatgcagcga catgttggtg
     8641 gtaattatgg attttaaaag ttttattatt ggtttagtag ttggtatatt tggtccttat
     8701 atggatgatt taattagaaa aaaattttta aagtcttcgg agaagaaaac agaaaaatct
     8761 gttaaaaaat aatcaaaact ataaatgatg aatctgaatc aaaattattt tgcgcatgta
     8821 aagaggagtc ttatagtaac gagtcaaaaa aggagtaact atgaatcgta atttagaacg
     8881 gtgttatcta ttctgactag gaatagatca taccagaggt agcttagaaa tagcagggac
     8941 attagaaatt gaagtaataa ataggatgtc gtaagtgtta ctatcaatga tttatttgtt
     9001 tcaagcttgc ctagggtgac agtaaaaaat caatttcctt tcaatagcat atttttagtg
     9061 ggcaggactc ttgttctgcc tattttttta tccaaaaagt gcagttggga gggagatagg
     9121 ctcatttggg aaggaagtcc agtttttgtt tagtgattgg ggtaagatag ttgttatcag
     9181 atgagtttat actcttcgaa aatcaaattc aaaccacgtc aacgtcgcct tgccgtatat
     9241 atgtgactga cttcgtcagt cctatctaca acctcaaaac agtgttttga gcagcctgcg
     9301 gctagtttcc tagtttgctc tttgattttc attgagtatt agggaaaagg agatgaatat
     9361 gaaatttggg aaacgtcact atcgtccgca agtggatcag atggactgcg gtgtagcttc
     9421 attagccatg gtttttggct actatggtag ttattatttt ttggctcact tgcgagaatt
     9481 ggctaagacg accatggatg ggacgacggc tttgggcttg gtcaaggtgg cagaggagat
     9541 tggttttgag acgcgagcca ttaaggcgga tatgacgctt tttgacttgc cggatttgac
     9601 ttttcctttt gttgcccatg tgcttaagga agggaaattg ctccactact atgtggtgac
     9661 tgggcaggat aaggatagca ttcatattgc cgatccagat cccggggtga agttgactaa
     9721 actgccacgt gagcgttttg aggaagaatg gacaggagtg actcttttta tggcacctag
     9781 tccagactat aagccttata aggaacaaaa aaatggtctg ctctctttta tccctatatt
     9841 agtgaagcag cgtggcttga ttgccaatat cgttttggca acactcttgg taaccgggat
     9901 taacattgtg ggttcttatt atctgcagtc tatcattgat acctatgtgc cagatcagat
     9961 gcgttcgaca ctagggatta tttctattgg gctagtcatc gtctacatcc tccagcaaat
    10021 cttgtcttac gctcaggagt atctcttgct tgttttgggg caacgcttgt cgattgacgt
    10081 gattttgtcc tatatcaagc atgtttttca cctccctatg tccttctttg cgacacgcag
    10141 gacaggggag atcgtgtctc gttttacaga tgctaacagt atcatcgatg cgctggcttc
    10201 gaccatcctt tcgattttcc tagatgtgtc aacggttgtc attatttccc ttgttctatt
    10261 ttcacaaaat accaatctct ttttcatgac tttattggcg cttcctatct acacagtgat
    10321 tatctttgcc tttatgaagc cgtttgaaaa gatgaatcgg gataccatgg aagccaatgc
    10381 ggttctgtct tcttctatca ttgaggacat caacggtatt gagactatca agtccttgac
    10441 cagtgaaagt cagcgttacc aaaaaattga caaggaattt gtggattatc tgaagaaatc
    10501 ctttacctat agtcgagcag agagtcagca aaaggctctg aaaaaggttg cccatctctt
    10561 gcttaatgtc ggcattctct ggatgggggc tgttctggtc atggatggca agatgagttt
    10621 ggggcagttg attacctata ataccttgct ggtttacttt accaatcctt tggaaaatat
    10681 catcaatctg caaaccaagc ttcagacagc gcaggttgcc aataaccgtc taaatgaagt
    10741 gtatctagta gcttctgagt ttgaggagaa gaaaacagtt gaggatttga gcttgatgaa
    10801 gggagatatg accttcaagc aggttcatta caagtatggc tatggtcgag acgtcttgtc
    10861 ggatatcaat ttaaccgttc cccaagggtc taaggtggct tttgtgggga tttcagggtc
    10921 aggtaagacg actttggcca agatgatggt taatttttac gacccaagtc aaggggagat
    10981 tagtctgggt ggtgtcaatc tcaatcagat tgataaaaaa gccctgcgcc agtacatcaa
    11041 ctatctgcct caacagccct atgtctttaa cggaacgatt ttggagaatc ttcttttggg
    11101 agccaaggag gggacgacac aggaagatat cttacgggcg gtcgaattgg cagagattcg
    11161 agaggatatc gagcgcatgc cactgaatta tcagacagaa ttgacttcgg atggggcagg
    11221 gatttcaggt ggtcaacgtc agagaatcgc tttggcgcgt gctctcttga cagatgcgcc
    11281 ggtcttgatt ttggatgagg cgactagcag tttggatatt ttgacagaga agcggattgt
    11341 cgataatctc atggctttgg acaagacctt gattttcatt gctcaccgct tgactattgc
    11401 tgagcggaca gagaaggtag ttgtcttgga tcagggcaag attgtcgaag aaggaaagca
    11461 tgctgatttg cttgcacagg gtggctttta cgcccatttg gtcaatagct agaaagagga
    11521 gaggatgaaa ccagaatttt tagaaagtgc ggagttttat aatcgtcgtt accataattt
    11581 ttccagtagt gtgattgtac ccatggccct tctgcttgtg tttttacttg gctttgcaac
    11641 tgttgcagag aaggagatga gtttgtccac tagagctact gtcgaaccta gtcgtatcct
    11701 tgcaaatatc cagtcaacta gcaacaatcg tattcttgtc aatcatttgg aagaaaataa
    11761 gctggttaag aagggggatc ttttggttca ataccaagaa ggggcagagg gtgtccaagc
    11821 ggagtcctat gccagtcagt tggacatgct aaaggatcaa aaaaagcaat tggagtatct
    11881 gcaaaagagc ctgcaagaag gggagaacca ctttccagag gaggataagt ttggctacca
    11941 agccaccttt cgcgactaca tcagtcaagc aggcagtctt agggctagta catcgcaaca
    12001 aaatgagacc atcgcgtccc agaatgcagc agctagccaa acccaagccg aaatcggcaa
    12061 cctcatcagt caaacagagg ctaaaattcg cgattaccag acagctaagt cagctattga
    12121 aacaggtgct tccttggccg gtcagaatct agcctactct ctttaccagt cctacaagtc
    12181 tcagggcgag gaaaatcccc aaactaaggt tcaggcagtt gcacaggttg aagcacagat
    12241 ttctcagtta gaatctagtc ttgctactta ccgtgtccag tatgcaggtt caggtaccca
    12301 gcaagcctat gcgtcagggt taagcagtca attggaatcc cttaaatccc aacacttggc
    12361 aaaggttggt caggaattga cccttctagc ccagaaaatt ttggaggcag agtcaggtaa
    12421 gaaggtacag ggaaatcttt tagacaaggg gaaagttacg gcgagtgagg atggggtgct
    12481 tcatcttaat cctgagacca gtgattctag catggttgca gaaggtgccc tactagccca
    12541 actttatcca tctttggaaa gagaagggaa agccaaactc acagcttatc taagttcaaa
    12601 atatgtagca agaatcaagg tcggtgattc tgttcgctat actacgactc atgatgccgg
    12661 gaatcaactt ttcctagatt ctactattac aagtattgat gcgacagcta ctaagactga
    12721 gaaagggaat ttctttaaaa tcgaggcgga gactaatcta acttcggagc aggctgaaaa
    12781 acttaggtac ggggtggaag gccgcttgca gatgattacg ggcaagaaaa gttacctacg
    12841 ttattatttg gatcaatttt tgaacaaaga gtaatgttcg tgtttttaga gttaaataat
    12901 ttttaaactg tgagaaagat tcttcttgca gttttttctt tacaattttt gaaaaacatc
    12961 tactatttat tcggttaaat tcttgtgttt tttggttttt tgtggtaaaa tgtgctcaag
    13021 taatacgaaa ggcgaacttt aaaatgtcaa aacaattgat ctattcggga aaagctaaag
    13081 atatctatac aactgaggat gaaaatctta ttatttcaac ttacaaggac caggcgactg
    13141 ctttcaacgg tgtcaagaag gagcagattg caggtaaggg agtcttgaat aatcagatct
    13201 catcttttat ttttgagaaa ttaaatgtgg ctggtgtggc gactcacttt gtggagaaac
    13261 tttcagacac ggaacaactc aataaaaagg ttaagattat tcctttggaa gtcgtgctcc
    13321 gcaactatac tgctggttcc ttttcaaaac gttttggtgt ggatgaggga atcgccttgg
    13381 agactccgat tgtcgaattt tactacaaaa atgatgattt ggatgatcca tttatcaatg
    13441 atgagcatgt gaaattccta cagattgcgg gtgaccagca gattgcctac ttgaaggaag
    13501 aaacgcgtcg tatcaatgaa ctattgaaag tctggtttgc tgagattggg cttaaattga
    13561 ttgactttaa gctagagttc ggttttgaca aggatggcaa gattatcttg gcagacgaat
    13621 tttcaccaga taactgccgc ttgtgggacg ctgatggcaa ccacatggat aaggatgttt
    13681 tccgtagagg attgggagaa ctaaccgacg tttatgagat tgtttgggaa aagttgcagg
    13741 aattgaaata atctgtttgc aacggaaaac cttcgtctct caactaaaag gactcaggct
    13801 gaaaaggtcc cccagacctt ttcactctgt agagaactag gtgaactaac agatgtttac
    13861 gaaattgtct gggaaaagtt gcagggttta aaataacaac ctcaaggctg tttgggaata
    13921 ttgcaagagc tgaaataaag gaataagaat tgatggataa acgtattttt gttgaaaaaa
    13981 aggctgattt tcaggtcaag tcagagagtt tggttagaga gctccagcac aacttgggac
    14041 tgtcaagctt gaaaagtatt cgtattgtgc aagtatatga tgtatttgac ttggctgagg
    14101 acttgtttgc acctgcagag aagcacattt tctctgagca gggaaccgac catgttttag
    14161 atgaagtatc tgtgcaggcg gatcttgcta actatgcttt ctttgccatt gaaagtctgc
    14221 cagggcagtt tgaccagcgt gcagcttcgt cacaggaagc cttgcttttg ttgggaagtt
    14281 cgagtgacgt gacagtcaac acagcccaac tttacttggt gaataaagat attgatgcga
    14341 ctgagttgga agctgtcaaa aactacctgc tcaatccagt tgattctcgt ttcaaggata
    14401 tcacgacagg gattgccaag caggagtttt cagagtcaga caagaccatt ccaaaattga
    14461 ctttctttga aagctataca gcagaagact ttgctcgcta caaggccgag caagggatgg
    14521 ccatggaagt ggatgatttg ctctttatcc aagactactt taagtcaatc gggcgcgtgc
    14581 caactgagac tgaactcaag gttttggaca cttactggtc tgaccactgc cgtcatacga
    14641 cttttgagac agagttgaaa cacatcgact tttcagcttc taaatttcaa aagcaattgc
    14701 agtcaaccta tgacaagtat attgccatgc gcgaggagtt aggtcggtct gaaaaaccac
    14761 aaaccttgat ggatatggcg actattttcg gtcgttatga gcgtgctaac ggacgattgg
    14821 atgatatgga agtctctgac gaaatcaatg cctgctcagt tgaaattgaa gtggatgttg
    14881 atggtgtcaa ggaaccttgg ctcctcatgt ttaaaaatga aacccacaac catccaacag
    14941 aaattgagcc atttggtgga gcggctacct gtatcggtgg agctattcgt gatccgttgt
    15001 caggccgttc ctatgtttac caagccatgc gtatttcagg tgctggtgat attacagcac
    15061 cgatttcgga aactcgtgct gggaaattgc cacaacaagt catttctaaa acagcagctc
    15121 atggttattc ttcatatggt aaccagattg ggcttgccac aacctacgtt cgtgaatact
    15181 tccacccagg ctttgtagct aaacgtatgg aacttggtgc cgttgttggt gcggctccca
    15241 agggcaatgt tgtccgtgaa aaacctgaag caggtgatgt gatcatcctt ctcggaggca
    15301 aaacaggtcg tgatggtgtc ggtggtgcga cgggctcttc taaggttcaa acagttgagt
    15361 ctgtagagac tgctggtgct gaggttcaaa aaggaaatgc catcgaagaa cgcaagattc
    15421 agcgcctctt ccgtaatggc aatgtcactc gtctgatcaa gaagtccaat gactttgggg
    15481 caggcggcgt ctgtgtggct atcggtgaat tggcagacgg tcttgaaatc gacctcaaca
    15541 aggtgcctct taaataccag ggcttgaatg gtacagaaat tgccatctct gaatcacaag
    15601 aacggatggc ggtcgtggtt cgtcctgaag atgtggatac cttcgttgcc gaatgtaaca
    15661 aagaaaatat tgatgctgtt gtggtggcga cagtaactga aaaaccaaat cttgtcatgc
    15721 actggaatgg tgagacaatc gttgacttgg agcgtcgttt ccttgacacc aatggtgtgc
    15781 gcgtggttgt cgatgccaaa gttgtggaca aggatgtcaa actcccagaa gagcgtcaaa
    15841 catctgctga aacactggaa tcagataccc ttacggttct atctgacctc aaccatgcaa
    15901 gtcaaaaagg attacagact atctttgact gctctgttgg acgctcaacg gttaatcacc
    15961 cacttggtgg tcgttaccaa ctcacaccaa ctgaggcatc tgtgcagaaa ttgccagttc
    16021 aacacggtgt gactcatact gcgtcggtca ttgctcaagg tttcaaccca tatgtagctg
    16081 aatggtctcc ataccacggt gctgcttatg cggttatcga agcaactgct cgtttggtgg
    16141 ctgctggtgc caactggtct aaggctcgtt tctcttacca agaatatttc gagcggatgg
    16201 acaagcaagc agagcgtttc ggtcagccag tagctgctct tctaggttct attgaagcac
    16261 aaattcagct tggcttgcca tctatcggtg gtaaggactc catgtctggt acctttgaag
    16321 aattgaccgt tccgccaacc ttggttgcct ttggggtgac gacggcagat agccgtaagg
    16381 tgctctctcc agaatttaaa gctgttgggg aaaatatcta ctacatccca ggtcaagccc
    16441 tctctgcaga gattgatttt gacttgatta agaaaaattt tgctcagttt gaagcaagcc
    16501 aagctgacca taaagtgacg tctgcatcag ctgtcaaata cggtggtgta gttgaaagtt
    16561 tggctcttgc tacctttgga aactatattg gtgcagaggt gaccttgcct gaacttaaaa
    16621 cagctttgac agctcaatta ggcggctttg tcttcacatc tcctgaagaa attgctggag
    16681 tagagaagat tggacaaacg aaagcagact ttacactgac tgtcaacggt gtgaagctag
    16741 atggacacaa gcttgacagt gcatttcaag ggacattgga agaagtttac ccaacagaat
    16801 ttacccaagc gaaagaacta gaagaagtac cagctgtggc atcagatgtt gtgattaaag
    16861 ccaaagaaaa ggttgaaaaa cctgtggttt acatcccagt ctttccagga accaactcag
    16921 aatatgattc agctaaggcc ttcgaaaaag aaggtgcaga ggtcaatttg gtgccattcg
    16981 tgaccttgaa tgaagaagct attgtcaagt cagttgaaac tatggttgac aatatcgaca
    17041 agactaatat tctcttcttt gccggtggat tctcggctgc ggatgaacca gatggttcag
    17101 ctaagtttat cgtcaatatc ctgcttaatg aaaaagtgcg tgtggctatt gatagcttta
    17161 tcgcccgtgg tggcttgatt atcggtattt gtaatggatt ccaagcctta gtcaaatcgg
    17221 gtctcctacc atacggaaac tttgaagatg ctaacagtac tagtccaacc ctcttctaca
    17281 atgatgccaa ccaacacgtg gccaagatgg tggaaactcg tattgccaat accaactcac
    17341 catggttggc tggtgtgcaa gtgggcgata tccacgctat tcctgtttcg cacggtgaag
    17401 ggaagtttgt cgtgacggct gaggaatttg cagagctccg tgacaatgga caaattttca
    17461 gccaatacgt tgactttaac ggtaaaccaa gtatggattc taagtacaat ccgaatggtt
    17521 ctgtccatgc catcgaagga attaccagca agaatggtca aatcatcggt aagatgggcc
    17581 actcagaacg ttatgaggat ggtcttttcc aaaatatccc aggcaataaa gaccaacacc
    17641 tgttcgcatc agcggttaaa catttcactg gaaaataaga cttacagatt ttctaataga
    17701 tagtatcagt aatgtaaaag tcatgtaaat ctagctcttg atgattacaa atgaaaatta
    17761 ggtataaaaa atgacatacg aagtaaaatc tcttaatgaa gaatgtggtg ttttcggtat
    17821 ttggggacat ccagatgctg ctaagttgac ctattttgga ctccacagtc ttcaacaccg
    17881 tggtcaggag ggggcaggaa tcctctccaa tgatcaagga caactgaagc gccatcgtga
    17941 catggggctt ttatcagaag ttttcagaaa tccagccaat ttggataaat tgacaggagc
    18001 tggtgcgatt gggcatgtgc gttatgcgac tgctggcgaa gcttctgttg ataacatcca
    18061 acccttcctc ttccgttttc acgatatgca gtttggtttg gctcataatg gaaatctgac
    18121 caatgcagcc tctctcaaga aagaactgga acaaagagga gcaattttca gcgcgacttc
    18181 ggactctgaa atcttggctc acctcattcg tcgcagtcat aatcctagcc tgatgggcaa
    18241 aatcaaggaa gcgctcagcc ttgtcaaagg tggttttgcc tatatcttgc tgtttgagga
    18301 caagttgatt gcggctcttg accctaatgg ctttcgtccg ctttctatcg ggaaaatggc
    18361 caacggagcg gtggttgttt cctctgaaac ctgtgctttt gaggtcattg gtgtcgagtg
    18421 gatttgtgat ttgaagccag gtgagattgt gatcattgat gacgagggca ttcagtatga
    18481 cagctataca gatgataccc agttggcgat ttgttctatg gagtatatct actttgcccg
    18541 ccctgattct aatatccacg gtgtcaatgt ccatacggca cgtaagagaa tgggagcgca
    18601 attggcgcga gaatttaagc atgaggcaga tattgtagtt ggtgtgccca attcttccct
    18661 aagcgcggct atgggatttg cggaagaatc aggcttacca aatgaaatgg gtctgatcaa
    18721 aaaccaatac acccagcgaa cttttatcca accgactcaa gaattgcggg agcaaggagt
    18781 gcggatgaaa ctgtctgctg tttcgggtgt tgtcaaaggc aaacgtgtgg tcatggtgga
    18841 tgattccatt gtacgtggaa caacctctcg tcgtatcgtt cagctcttga aagaagcggg
    18901 tgcgactgag gttcacgttg ccattggaag tcctgcacta gcgtatccat gtttctacgg
    18961 gattgatatc cagacccgtc aggagctgat tgcagccaat catacggtcg aagaaactcg
    19021 ccaaatcatt ggtgcggaca gtctgactta tctttcaatt gatggcttga ttgagtcgat
    19081 tggtatcgaa acagatgcgc cgaacggtgg tctctgtgtc gcttactttg acggtgacta
    19141 cccaacgcct ctctatgact acgaagaaga ctatcgtaga agtttggaag aaaagaccag
    19201 tttttacaag taggcgacag attctccatt aaagaaaagg aaaaaacaaa tgacaaataa
    19261 aaatgcatat gcctcacgtc tcactactga ctaaaggctt aagcatttag tcagtagacg
    19321 ctttgtccta taggatcaaa gctagagccc tgactagtat ttttagataa aaagatggtt
    19381 tatctaaaaa tacgtcgcag tctttctcaa aaaaagaaaa ggaaaaataa aatggcaaat
    19441 aaaaatgcgt acgctcaatc tggtgtggat gttgaagcgg gttatgaagt tgttgaacgg
    19501 attaaaaagc acgtggctcg tacggagcgt gcaggtgtca tgggagctct tggtggcttt
    19561 ggtggtatgt ttgacctttc caagactggg gttaaagaac ccgtcttgat ttcagggact
    19621 gacggtgtcg gaaccaagct catgttggct atcaagtacg acaagcacga taccatcggg
    19681 caggactgtg tggccatgtg tgtcaacgac atcattgctg caggtgcgga acccctctat
    19741 tttctcgact acgtagcgac agggaagaat gaaccagcta agctagaaca agtggttgct
    19801 ggtgtggcag aaggttgtgt gcaggctggt gctgccctca tcgctgggga aacggctgaa
    19861 atgccgggca tgtacggcga agacgactat gacttggctg gttttgcggt cggtgtggct
    19921 gaaaaatctc aaatcattga cggttcaaag gtggtagagg gagatgttct tctcggactt
    19981 gcttcaagtg ggattcactc aaatggttac tctttggttc gtcgtgtctt tgcggattac
    20041 acaggtgagg aagtcctacc agaattggaa ggcaagaaac ttaaggaagt tctacttgag
    20101 ccgactcgta tctatgtcaa ggctgtcttg ccgctcatca aagaagagtt ggtcaacggc
    20161 attgcccaca tcacaggtgg tggctttatc gaaaatgtcc ctcgtatgtt tgcagatgac
    20221 ctagctgctg aaattgatga aagcaaagtt ccagtgcttc caattttcaa agcccttgaa
    20281 aaatatggtc agattaaaca cgaagaaatg tttgaaatct tcaatatggg tgtgggactt
    20341 atgttggcgg tcagccctga aaatgtagag cgtgtaaaag aattgttgga tgaagcagtc
    20401 tatgaaattg gtcgcatcgt caagaaagaa aacgaaagtg tcattatcaa atgaaaaaaa
    20461 tagcggtttt tgcctctggt aatggctcaa attttcaggt gattgccgaa gaatttccag
    20521 tggagtttgt cttttcagac catcgtgatg cctatgtgct tgagcgtgca aagcagctcg
    20581 gcgttctgtc ctatgctttt gaactcaagg agtttgagag caagacagac tacgaagcag
    20641 cccttgtcga actcttggaa gaacaccaga ttgacttggt ttgcctagca ggctacatga
    20701 aaatcgttgg accaacctta ttgtcggctt atgaaggtcg gattgtcaac attcatccag
    20761 cctacttgcc agaatttcca ggagctcatg ggattgagga tgcttggaat gctggcgtgg
    20821 gtcagtctgg tgtgaccatt cactgggtgg attcgggtgt ggatacaggc caggtcatca
    20881 aacaggttcg tgtgccacga ctagctgatg ataccattga cagatttgaa gctcgcatcc
    20941 atgaagcaga gtacaggttg tatccggaag tagtgaaggc tctatttaca gattgacttt
    21001 ttgatgattc atatgatatc tttgatttta aattggagtc agtgtttgtt gaagacggct
    21061 tcaaacggag gtatttgtaa tgttagaatc taaaaaaaca actcgatatg tattttatgt
    21121 ctatctgatg ttattaactt ggggaatctt atttaagttt gaaacaaatc ctgaatttat
    21181 agcatttttc ttagctccaa ggtatatcaa ttggattcca ttttcagaac cactaatagt
    21241 cgatggaaaa attgtttttg ctgaaatgtt atttaatctg atttccttta ttccattagg
    21301 tgtttgtttc cctttgataa aaactaattt atctagttta agaatagtcg ggacaggttt
    21361 cttgattagt ttattgtttg agtgcttaca gtatatttta gcaataggta taacagatat
    21421 aacggatttg actttaaata cgctaggtgt ctgtgtaggc ttactgattt atcaaatttt
    21481 tataagagtg ttcaaatcac agactagaaa atggatcaat atcttaggta tgcttagcct
    21541 tggttttgct tatcttgttt tactgttact gcatttaatt ggtgtttaac taatgattaa
    21601 aaaggagaat ataatgacta aacgcgtctt aatcagcgtc tcagacaaag cgggcattgt
    21661 tgaatttgcc caagaactaa aaaaacttgg ttgggagatt atctcgacag gtggaactaa
    21721 ggttgccctt gataatgctg gggtggagac cattgcgatc gatgatgtga cgggcttccc
    21781 agagatgatg gatggccgtg ttaagactct tcatccaaat atccacgggg gtcttctcgc
    21841 tcgtcgtgat ttggatagcc acttggaagc agccaatgag aaccaaattg agcttattga
    21901 ccttgtagtg gtcaaccttt acccatttaa ggaaactatc cttaaaccag atgtgactta
    21961 tgctgatgca gttgaaaata tcgatattgg tgggccgtct atgcttcgtt cagcagcgaa
    22021 aaatcatgcc agtgttacag ttgtggtaga tcctgctgac tacgctgtgg ttttggacga
    22081 attggcagca aacggcgaaa cctcttatga aactcgccaa cgtttagcag ccaaagtatt
    22141 tcgtcacaca gcggcttatg acgccttgat tgcagaatac ttcacagctc aagtgggtga
    22201 aagcaagcct gaaaaactca ctttgactta tgacctcaag caaccaatgc gttacggtga
    22261 gaatcctcaa caagacgcgg acttttacca gaaagctttg cctacagatt actctattgc
    22321 ttcagccaaa cagctcaacg ggaaagaatt gtcatttaat aatatccgtg atgcagatgc
    22381 tgctatccgt atcatccgtg acttcaaaga tagtccaacc gttgtggctc tcaagcatat
    22441 gaatccatgt ggtatcggcc aggcggatga catcgagacg gcttgggatt atgcttatga
    22501 gtctgaccca gtgtctatct ttggtggtat cgttgttctt aaccgtgagg tggatgctgc
    22561 gacagctgag aagatgcacg gcgttttcct cgaaatcatc attgcaccaa gctatacgga
    22621 tgaagcgcta gctattttga tcaataaaaa gaaaaacttg cgtatccttg ccttgccatt
    22681 taatgctcaa gaggctagcg aagtggaagc agaatacaca ggtgtagtcg gtggacttct
    22741 cgtgcaaaat caagacgtgg tcaaggaaag cccagctgac tggcaagtgg tgaccaaacg
    22801 tcagccaacc gagacagaag cgactgctct tgagttcgct tggaaggcta tcaagtacgt
    22861 caaatcaaat ggtattatcg tgaccaacga tcacatgaca cttggtgttg gtccaggtca
    22921 aaccaaccgt gtggcttctg ttcgccttgc cattgaccaa gccaaagatc gtctggacgg
    22981 ggcggtcctt gcttcagatg ccttcttccc atttgcggat aacgtggaag aaatcgccaa
    23041 agcaggaatt aaggccatca tccagcccgg tggctctgtc cgtgaccaag aatccatcga
    23101 agcagcggat aaatatggct tgactatggt ctttacaggt gtgagacatt ttagacatta
    23161 agaagataaa agggaagaaa acagtttctt tccttttttg gcttaaaatg ctaactgaaa
    23221 caagattaaa acgaactttt ttgatataat gttggtaaat aattcgcaaa agaggttgag
    23281 gaatgaaact gcttgttgtc ggttctggtg gtcgtgagca tgcgattgct aaaaagttac
    23341 ttgaatcaaa agacgtggaa aaagtctttg tagctcctgg gaatgatggg atgactctgg
    23401 atggtttgga attggtaaat atctctattt ccgaacattc taaattgatt gacttcgcaa
    23461 agaccaatga tgttgcttgg acctttatcg gtccagatga tgcccttgct gctggtatcg
    23521 tggatgattt taaccaagct ggacttaagg cctttggtcc gactagggct gcagcggagc
    23581 tggagtggtc caaggatttc gccaaggaaa tcatggtcaa atacggcgtt ccgacagcaa
    23641 catatggcac attttcagat ttcgaggaag ccaaggccta tatcgaaaag catggtgcgc
    23701 ctatcgtagt caaggcggat ggcttggcac ttgggaaggg tgtcgtcgtt gctgagacgg
    23761 ttgagcaagc ggtcgaagcc gctcatgaga tgcttttgga caataaattt ggtgactcag
    23821 gtgcgcgcgt ggttattgag gaattccttg aaggagagga attttcactc tttgcctttg
    23881 tcaatggtga taagttctac atcatgccaa cggctcagga ccacaaacgt gcctatgatg
    23941 gcgacaaagg gcctaacacg ggtggtatgg gtgcctatgc gccagtccca cacttaccac
    24001 agagtgtagt tgatacagcg gttgacacca ttgtcaagcc agttctagaa ggggtgatta
    24061 aagaaggtcg cccttatctg ggagttcttt acgcagggct tatcctgaca gctgatggac
    24121 cgaaagtcat tgagttcaac gctcggttcg gagatccaga aactcagatt atcttgcctc
    24181 gcttgacctc tgactttgct caaaatatca cagatatcct ggatagcaag gagccaaata
    24241 tcatgtggac ggacaagggt gtgactctgg gtgtggttgt cgcatccaag ggctacccgc
    24301 tagactatga aaggggcgtt gagttgccag caaagacaga aggcgatgtc atcacctact
    24361 atgcaggggc taagtttgcg gaaaatagca gagcactgct ctcaaacggc ggacgagttt
    24421 atatgctcgt taccacagca gataccgtca aagaagccca agccagcatc taccaagaac
    24481 tatcccaaca aaaaatagaa ggactcttct accgaacaga tatcggaagc aaggcaatta
    24541 agtaaagata taagaataac gcgccgtagt cgccaaacac gataatggtc gtcgtggtga
    24601 aaagaccaga acagtgaatg ttctggtcag ggggaaactt ggagacctta ggctcaaagt
    24661 ttaggaatga aaccgaaggt ttgcttccgc ctccatcacc taagaccatt atcaaaaaga
    24721 aaaataaaaa ttcacaaaat acgttaatga tcgtatggtt tgcgagcgtt agcgagctaa
    24781 tatagaacaa tcaccgccgt tgtgaaagaa cgattggatg ataatccaat cgttcaggga
    24841 aattggaaga ccttgggttt ccaatttagg catgagacac ctttggtggc tgctgccgtc
    24901 cctcacaagc taaggtgatt gttgaaaaag aggaaaaagg agaagaaatg aaaccagtaa
    24961 tttccatcat catgggctca aaatccgact gggcaaccat gcaaaaaaca gcagaagtcc
    25021 tagaccgctt cggtgtagcc tacgaaaaga aagttgtttc cgcacaccgt acaccagacc
    25081 tcatgttcaa acatgcagaa gaagcccgta gtcgtggcat caagatcatc atcgcaggtg
    25141 ctggtggcgc agcgcatttg ccaggcatgg tagctgccaa aacaaccctt ccagtcattg
    25201 gtgtgccagt caagtctcgt gctcttagtg gagtggattc actctattct atcgttcaga
    25261 tgccgggtgg ggtgcctgtt gcgaccatgg ctatcggtga agctggagcg actaacgcag
    25321 ctctctttgc cctccgtctc ctctctgtag aagataagtc cattgcggat gcacttgcca
    25381 actttgctga agaacaagga aaaatcgcag aggagtcgtc aaatgagctc atctaaaaca
    25441 atcggaatta tcggtggcgg tcaactgggt cagatgatgg ccatttctgc tatctacatg
    25501 ggccacaagg ttatcgcgct ggatcctgcg gcggattgcc cggcctctcg cgtggcggaa
    25561 atcattgtgg caccttataa cgatgtggac gccctccgtc agttggcaga ccgttgcgat
    25621 gtcctcactt acgagtttga aaatgtcgac gctgacggtt tggatgccgt tatcaaggat
    25681 ggacaactcc ctcaaggaac agatctgctc cgcatttcgc aaaatcgtat ttttgaaaag
    25741 gactttttgt caaacaaggc tcaagtcact gtggcaccct acaaggtcgt gacttctagc
    25801 ctagacttgg cagatatcga cttgtcgaaa aactatgtcc tcaagactgc gactggtggc
    25861 tacgatggtc atggacaaaa ggttattcgt tcagaagcag acttggaagc agcctatgcg
    25921 ctagcagact cagcagactg cgtcttggaa gaatttgtca actttgacct tgagatttct
    25981 gtcatcgtgt caggaaatgg caaggaggtg acgtttttcc cagttcagga aaatatccac
    26041 cgcaacaata tcctgtctaa gaccatcgta ccagcccgca tttctgaaag tctagcagac
    26101 aaggctaaag ctatggcagt gcgaatcgca gaacaactca acttgtctgg aactctctgt
    26161 gtggaaatgt ttgcgactga tgatgacgtt attgtcaatg aaatcgcccc acgaccacat
    26221 aactctgggc actattctat tgaagcctgt gatttctctc agtttgacac ccatattctc
    26281 ggtgttctcg gagcaccatt accagtcatc aaactccatg cgccagccgt tatgcttaat
    26341 gtcctcggtc agcatgtcga ggctgctgag caatatgtga cagaaaattc aagcgcccac
    26401 ctccacatgt atggtaaaat agaagcgaag cacaaccgca agatgggaca tgtgaccttg
    26461 tttagtgatg taccggatag tgtggatgag cttggggaag ggattgattt ttaggacaag
    26521 tctatgatac aaattatcgt taatacattt attgaaaagg ataagactgg agcagttgtt
    26581 gaagtgttgt atgccagtgc tgaccaagat aaggtacaag ctaaatatga agaactagct
    26641 gcacaatacc ccgaaaatta tttagctatc tatgatgtac cgctggatac ggatttgaat
    26701 acactagatc attacccgtc tgtgtttatt gaaaaagagg agtttgagta gaaatcttgg
    26761 tttacctaga tagcttattc ccaacagctt aagaagaaag gaaaaattaa cacatgatca
    26821 accgttactc tcgccctgag atggcgaata tttggagtga agaaaataaa taccgtgctt
    26881 ggcttgaggt ggaaatcctc tctgacgagg catgggctga gttgggggaa atccctaagg
    26941 aagatgtggc tttgattcgc aagaaggcgg actttgacat cgaccgtatt ttggaaattg
    27001 agcaggagac gcgccacgat gtggtggctt tcacgcgtgc ggtttctgag actcttggtg
    27061 aagagcgcaa gtgggttcac tatgggttaa cttctaccga cgtggtggat actgcttatg
    27121 gttacctcta caagcaggcc aacgacatca tccgtcgtga ccttgaaaac ttcactaata
    27181 tcatcgctga caaggccaag gagcacaagt tcaccatcat gatggggcgt actcatggtg
    27241 tgcacgctga gccgacaacc tttggtctta aattggcaac ttggtacagc gaaatgaaac
    27301 gcaatatcga gcgtttcgag catgcagctg ctggtgtaga agctggtaag atttctggtg
    27361 cggttgggaa ctttgccaat atcccaccat ttgtagagga gtatgtctgc gataaacttg
    27421 gcatccgtgc ccaagaaatc tctacacaag tccttcctcg tgaccttcac gctgagtact
    27481 ttgcggttct tgccagcatt gcgacttcaa tcgaacgtat ggcgactgag attcgtggtc
    27541 tacaaaaatc tgagcaacgc gaagtagaag agttctttgc taaagggcaa aaagggtctt
    27601 cagcaatgcc tcacaaacgc aacccaatcg gttctgaaaa tatgactggt ctggcgcgtg
    27661 tcattcgtgg tcacatgatt acggcttatg aaaacgtcgc tctctggcac gaacgcgata
    27721 tttctcactc atcagctgag cgtatcatca caccagatac gaccattttg attgactaca
    27781 tgctcaaccg ttttggaaat atcgtcaaga acttgacagt cttcccagaa aatatgatcc
    27841 gaaacatgaa ctcgactttt ggtcttatct ttagccaacg ggctatgttg acattgattg
    27901 aaaaaggcat gacccgtgag caagcctatg acttggtgca accaaaaaca gcctactctt
    27961 gggacaacca agtagacttt aaaccacttc ttgaggcaga ttcagaagta acatcacgtc
    28021 tcactcaaga agaaatcgat gaaatcttca acccagttta ttacaccaaa cgagtggatg
    28081 atatctttga acgtcttgga ctaggtgatt aattaaaaaa taaacagcga gcttcaatct
    28141 cgctgtttat tttttatcga aaagacttag tcttcttttc ttttagtgag tccataggct
    28201 gctagtgtgg acatgagtcc tgcgactact agtcctgcag aatcgtgagt tcctgtttca
    28261 ggaagttttt tctctgttac cacaggagct ggatcttgag gaagagcttt gctttcctca
    28321 gcaggagcag ttgatggagc tggttggctt gggatttcta gttttggttt ttcttcagca
    28381 atagcggctt gtccgttttc atcgcctaca tgtgttacca tagttccgac ttcgactatt
    28441 tgagtaacgg cttcctgtgc tacgacacta tttacaagtg ttttcacttc cttaccatcg
    28501 gcagaagtgc tcacagagta gaagttgcta cgatgtccat tgacgccctt agtaatgact
    28561 tgtgtttttc ctttgagtaa gagtggattt tcacgagtca ctgtggtaaa tggaatttct
    28621 tctttttgga tatccagtct aggttttacc tcagtagttg gtgcaagacc actttcatca
    28681 cccttgtgag ttacaggagc gccaacttca accacttggt ttataacttc tttggttacc
    28741 tggctatcaa ggactgtttc tgttgttttt ccattttcag tgagtacaga gatgtaatga
    28801 gttcgttcac ctttgactcc tgctgtgata atattttcct gaccagctgg gaggttagga
    28861 ttttctttct tgataacttc aaatggaatt tcttcagttc ttgtgatgag ttctggtctg
    28921 gtttcaacat tggcagccac ttcattttca tctaggcttc ctgaatgagt tgcagctggt
    28981 ttgaggcctt gaagagcggc ttttaggttg gctacaagtg tgtcaagctc agcttgttta
    29041 ttacggttga ggttgtaatt tagagctgtt ttagctgcgt caagggtctc aagactttct
    29101 ttactatatc cttctaagtt tgtaggaatt ttagctaatt cttcgcggag agcattataa
    29161 ttagcacgga agtagtcttt gttgtggtct gcaaaggcag tcatgagttc aaagatttcc
    29221 tcttccttgt attcagcgct tggtctatct gcccagattg aaagcatact tccgactgtt
    29281 ggaagatcta cttcaggata tttggtagaa gctagttgat tgaatggtgt ttttccagta
    29341 ttctcaatag ctttcttgag gaaaccacca ccatcttctg gtttttgacc aagaatgtag
    29401 taccagtcac cgttggtatt caagaattta tagcctttgc ttgctaggta ttgaggtgat
    29461 gcgaggttat atccccacca gcctttagac cagtaagaaa tcaagacatc tttgtcaaac
    29521 tgaacatcgt ccttgtcttc atagtagaag ccatcgttga aggccattgg ttgaagccct
    29581 ctttctttgg ccatagctgc gagggtgttg gcatattcgg caaatttgcc atagagttga
    29641 taccacttga ggtagtacca gccttgggca ctagtcgcat cgttggcgta ttcgtcagta
    29701 ccaaagttga aaatctttgt tttacctgca aagaagtcca tgtatttacc gatgagggct
    29761 tttacaaagt tcatcgcttc ttcgtttttc aagtccatag ttgtttttga aactttatca
    29821 aagtgggctt gaggattttt aatacctaat ttttccatgg caaccagcat agcatccatg
    29881 tgacctggac tgttaatagc tgggatgaga ccgatgtcct tagatttagc gtattcaatt
    29941 agctctgtta cttctgcctg tgttagtgca gtaccgtttg gatcgtcgta gtaagcttta
    30001 gttccttcga taatagcttt tttaacgtca tcactagcat aggtttttcc gttggcagta
    30061 atggtcatat catcgagtag aaagcgaagt ccgtcatttc ctagaaggag atggacatca
    30121 gaatatccga gctcactggc cttgtctacg atgcgtttga gctggttcag agtaaagtat
    30181 ttgcgtccag catcgattga gattaccttg tttttggcaa gtttttcaac ctcacgttta
    30241 gcttcttctt ctttttgagc ttcaggcgtg agggtcaagt tgttgacagt ttcttgaagt
    30301 ttagcaatgg cttgatcaat cgtatcttgt tgggcacggc taaggttgct atcgagagag
    30361 cgaatagctt tttcagcttc ttttacggcc gtgacgcttt ctgcagtata acggttcagg
    30421 tcttttggta cctcgttaag tgcttgctct gcagattcat aatcagctgc gaagtattca
    30481 gcgttggcat ttgcaaaatg acgcatgagt ttgaagaggc gtgatggtga ataacgtgca
    30541 gatggagtgt cagcccaagc agctaccata ccaccgatga ttgggatatc agctccttct
    30601 gtttttggta cagaagtgat tggtgtgttt ttaataccat tgagcccctg atcgagattg
    30661 taccagcctt ggccatcagc gtttcgtcca agaacgtagt accaagcatc attggtatta
    30721 aggatttggt gacctttttc agctagtagt ttagaagaag cgacatcgta gcctccccaa
    30781 ccaccagtcc acatagaaac gatgatgtct ttgtcaaaac taccaaagct tgtgtcgcta
    30841 ttgtagtaga taccgtcgtt aaaggccatt ggtttgagac cgtgcgattt tacaatacga
    30901 gcgaggtcat tggcgtaggc aataaatttt tcatagcctt ttacagggta gccttcgttt
    30961 ggatagtatt tatcagcttg aagcacactc caacctttag catctgtcgc atcattggca
    31021 tattcatcaa gtccgatgtt gaagatttca gtctttttcg cgaaataagc agcatacttg
    31081 tcgataaggg cttttgtaaa agcgacagct tgttcgttgt caagatcgac agtacgggct
    31141 gatttcttcc caaaatagct aaagttaggg ttttggattc ccaattcttt catggcattg
    31201 agaatcgcat ccatgtgtcc aggactattt actgtcggaa tgagaccgat acctttatct
    31261 ttggcatagt taatcagatc tgtcatttga ctttctgtta agtgattgcc gtttggatcg
    31321 ttgtaataat catttgtacc tttttcaatg gcgcgtttga catcgtcact ggcataggtc
    31381 ttgccgttag ctgtgatgct catatcgtcc aacatgaaac ggagtccatc atttccgact
    31441 aataggtgta aatcagtgta gccataatgt ttcgctttat cgatgatttc cttgagctgt
    31501 tctggtgaga aatatttacg tccagcatca atagaaacaa ttttcttttt cgctagtttt
    31561 tcatttacag ttgcagcacg ttcctttcct gcctctgttt ccggtttgtc agcctctgct
    31621 ttcgcttcat cttttttagc tggtttatcc ttgtcagtct tgtctgtatt tgactcttta
    31681 gaatcaacct ctttcgcttc ttccttttta gggctagctt cttctgcctt tttattagca
    31741 gtttcttttt cagcagaagt tggagttacc acttctgctt tatcactagg agttgaacta
    31801 acttcctctt gtggtttttc ttctgttttt ggaagactag ctaccttatc agtagctgga
    31861 gtttctgttt ctacagtttt tggagcttct ggttgaagct ctgctttagg tgtttcctca
    31921 gtccgatttt cggatgattg aggggaatca gaaaccgtat ggatggtcgg ttggttttct
    31981 gtagtagtag gagtaactcc atcggctgca acagtctgtg cttggaaggc aaatccaatt
    32041 agaacagaag ctgctcctac agcgtattta cgaatagaaa aacgctgttg tttttcatgt
    32101 ttcattgcaa aacctcctga ttgcattgtt atattgatag cgattatata aatcaacgcc
    32161 tttattttat ttcttatatt aacgagagtc aagaggagat gacaaaaaac tataataagt
    32221 ataaaaaaat ataaaattta aacttaagat ttcagattgg tcggaaaaaa tgaatatata
    32281 tatctagtat aatttttggt tctatttcta taaaatattc cacaaattat agaatcttcc
    32341 aaaaataggt aagcgctacc tttttggtgt ggtataataa gcatagaaaa agcccaagcg
    32401 attagctcag gctttcttct tagtgatcac ggtcacatga gataaattta atcttgtagt
    32461 aatcagatcg tttgtaagtt tcactgtatt ctaaaacttg gccagttgat tcgagtttgg
    32521 tgattttagt ttgtaggaca gtagggaatt gttcatcgac tccgaggact gaagccgcat
    32581 gttctggagt tggaaagact atttcgttga tttcttcaaa gtgttcatca ttcatgtgaa
    32641 tgtggtagtc taacttgaaa cgattataga tagaactata gtattcaagg tttggataat
    32701 ttgcgttgat atattgttct gggatgtagg atgtatggta gatataaacg acaccgtttg
    32761 attcgcggat acgttcaatc ttgtagtaga attgatcgcc gcgtagaccc aatttttcca
    32821 agtaaacaag cttgtttccg cgttcaattg aaagaacagt taccttatca tctttagcat
    32881 tgaagagttc aatatctgaa aactctacaa gcttgtgttt gcgcgcacgt gaaacgaagg
    32941 ttccttttcc ttgttggcgg acaatatagc catctttggc aaggtcgttt aaggcgcgaa
    33001 caactgtgat agagctgaca tcgtacattg aaatgagttc tgcttcagtg taaaatttat
    33061 ctccactgct aaactgccca gagatgattt tattttttaa ttcgtctttt atgtattgat
    33121 atttgggaat tgccatattt ttacctctat tttccttctc catagaaaat tttaccataa
    33181 aagcgaaaaa aatagtagtc ttttgaataa aaaattagaa taatagtttg
//
LOCUS       DAVKQB010000023        32980 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKQB010000023 DAVKQB010000000
VERSION     DAVKQB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.023"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(24..182)
                     /locus_tag="VW528_001054"
                     /pseudo
     CDS             complement(24..182)
                     /locus_tag="VW528_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(455..1144)
                     /gene="rplA"
                     /locus_tag="VW528_001055"
     CDS             complement(455..1144)
                     /gene="rplA"
                     /locus_tag="VW528_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9911884.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1352..1777)
                     /gene="rplK"
                     /locus_tag="VW528_001056"
     CDS             complement(1352..1777)
                     /gene="rplK"
                     /locus_tag="VW528_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9911885.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            1909..2046
                     /locus_tag="VW528_001057"
     CDS             1909..2046
                     /locus_tag="VW528_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911886.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(2086..2802)
                     /locus_tag="VW528_001058"
     CDS             complement(2086..2802)
                     /locus_tag="VW528_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9911887.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKTKNEDGETKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(2805..3275)
                     /locus_tag="VW528_001059"
     CDS             complement(2805..3275)
                     /locus_tag="VW528_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9911888.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVVGDHQYFEGYSLFLA
                     KEHVSELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSSEVDKLLEIKE"
     gene            complement(3277..4314)
                     /locus_tag="VW528_001060"
     CDS             complement(3277..4314)
                     /locus_tag="VW528_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9911889.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREIADYLVKTLEGFGYQPVRTSK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKMDRIGGFPWNMIEGENCTI
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTSEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRIYKEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSTLVD"
     gene            complement(4518..5843)
                     /gene="brnQ"
                     /locus_tag="VW528_001061"
     CDS             complement(4518..5843)
                     /gene="brnQ"
                     /locus_tag="VW528_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9911890.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASVAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6014..6106)
                     /locus_tag="VW528_001062"
     CDS             complement(6014..6106)
                     /locus_tag="VW528_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9911891.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(6149..6301)
                     /locus_tag="VW528_001063"
     CDS             complement(6149..6301)
                     /locus_tag="VW528_001063"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9911892.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LTDSYD"
     gene            complement(6461..7039)
                     /locus_tag="VW528_001064"
     CDS             complement(6461..7039)
                     /locus_tag="VW528_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9911893.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7093..8493)
                     /gene="pepV"
                     /locus_tag="VW528_001065"
     CDS             complement(7093..8493)
                     /gene="pepV"
                     /locus_tag="VW528_001065"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9911894.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8506..9111)
                     /locus_tag="VW528_001066"
     CDS             complement(8506..9111)
                     /locus_tag="VW528_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9911895.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVCEKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            9282..10082
                     /locus_tag="VW528_001067"
     CDS             9282..10082
                     /locus_tag="VW528_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9911896.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKESSSQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTYLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSTDSPSN
                     YIVFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(10260..11093)
                     /locus_tag="VW528_001068"
     CDS             complement(10260..11093)
                     /locus_tag="VW528_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9911897.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLQGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEVLDGEYGLEYPKEIQSMRMTQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSKGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRREDNYQRHNLKLLASLIEKHRYVEDVKNFFDF
                     L"
     gene            complement(11083..12927)
                     /gene="uvrC"
                     /locus_tag="VW528_001069"
     CDS             complement(11083..12927)
                     /gene="uvrC"
                     /locus_tag="VW528_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9911898.1"
                     /translation="MNNLIKPKLELLPTSPGCYIYKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNEHYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKNDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVTAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(12966..13667)
                     /locus_tag="VW528_001070"
     CDS             complement(12966..13667)
                     /locus_tag="VW528_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9911899.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWPQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETNE"
     gene            complement(13746..14978)
                     /gene="murN"
                     /locus_tag="VW528_001071"
     CDS             complement(13746..14978)
                     /gene="murN"
                     /locus_tag="VW528_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9911900.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQNKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14983..16203)
                     /gene="murM"
                     /locus_tag="VW528_001072"
     CDS             complement(14983..16203)
                     /gene="murM"
                     /locus_tag="VW528_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9911901.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(16315..17094)
                     /locus_tag="VW528_001073"
     CDS             complement(16315..17094)
                     /locus_tag="VW528_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9911902.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17163..18824)
                     /locus_tag="VW528_001074"
     CDS             complement(17163..18824)
                     /locus_tag="VW528_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9911903.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(18999..21221)
                     /gene="recJ"
                     /locus_tag="VW528_001075"
     CDS             complement(18999..21221)
                     /gene="recJ"
                     /locus_tag="VW528_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9911904.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21498..22256
                     /locus_tag="VW528_001076"
     CDS             21498..22256
                     /locus_tag="VW528_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9911905.1"
                     /translation="MVLVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDRMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            22296..23060
                     /locus_tag="VW528_001077"
     CDS             22296..23060
                     /locus_tag="VW528_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9911906.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTNKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23073..23750
                     /locus_tag="VW528_001078"
     CDS             23073..23750
                     /locus_tag="VW528_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9911907.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGATLLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23760..24419
                     /locus_tag="VW528_001079"
     CDS             23760..24419
                     /locus_tag="VW528_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9911908.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            24677..25867
                     /locus_tag="VW528_001080"
     CDS             24677..25867
                     /locus_tag="VW528_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9911909.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(26188..27069)
                     /locus_tag="VW528_001081"
     CDS             complement(26188..27069)
                     /locus_tag="VW528_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9911910.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(27208..28536)
                     /gene="vncS"
                     /locus_tag="VW528_001082"
     CDS             complement(27208..28536)
                     /gene="vncS"
                     /locus_tag="VW528_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9911911.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(28533..29189)
                     /gene="vncR"
                     /locus_tag="VW528_001083"
     CDS             complement(28533..29189)
                     /gene="vncR"
                     /locus_tag="VW528_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9911912.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(29238..29321)
                     /gene="pep27"
                     /locus_tag="VW528_001084"
     CDS             complement(29238..29321)
                     /gene="pep27"
                     /locus_tag="VW528_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9911913.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(29501..30880)
                     /gene="vex3"
                     /locus_tag="VW528_001085"
     CDS             complement(29501..30880)
                     /gene="vex3"
                     /locus_tag="VW528_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9911914.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(30932..31579)
                     /gene="vex2"
                     /locus_tag="VW528_001086"
     CDS             complement(30932..31579)
                     /gene="vex2"
                     /locus_tag="VW528_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9911915.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(31592..32869)
                     /locus_tag="VW528_001087"
     CDS             complement(31592..32869)
                     /locus_tag="VW528_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9911916.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9316 a         7538 c         6177 g         9949 t
ORIGIN      
        1 cgtgccgttc ggcatacggc ggttcaacta acttttaacg catgtcgttc aaggtaataa
       61 tccaaacacg aaaccagtcc acgtttttca aggactggtt ttgatatagc acgtttaagt
      121 accgacttct gagctactaa ttgatagtgg tcgccccagc cagatacctt atccgctata
      181 taggccttat tatcacgttc ctgttcgtag agccacgatt tcgctatccc ttcctctgac
      241 gattcttatt tccagcgtca gacttaaaac ggtctatccc cagaccgttt taatccgcta
      301 cctcacgata gtcagacttg ggaatcgcta ttgggttcac cggtagtagg tgcccaccga
      361 ggacttacac ctcagatata cgacatgccc gtcgtacatc aaaaaaagtt gagacatagt
      421 gtcttcaact tttttacatt actttaacta ctgattaaag tgagtttacg tcaactttga
      481 taccaacacc ttgagtagtt gtgatagtca agtttgttac gtaagttcct ttagctgtag
      541 ctggttttgc tttttggatt gtttcgttga aagctttaaa gttttcaacc aatttttcag
      601 cttcaaatga tactttaccg atgattgctt gaacgttacc tgcacggtca gcacggtaag
      661 tgattttacc acctttagac tcttcaactg ctttagcaac atccattgtt acagtaccag
      721 ttttagggtt tggcatcaag ttacgtggtc caaggacacg tccaagacgt ccaacaagag
      781 ccatcatatc aggtgtagcg ataactacgt cgaagtccaa ccaaccgtcg ttgattttag
      841 caacaaggtc atcttcacca acaaagtctg caccagcagc ttttgcttct tcagcttttg
      901 caccacgtgc gaaaacaaga acacgtgaag ttttaccagt accgtttggc aataccattg
      961 ctccacggat ttgttggtca gcttttttaa cgtcgatgtt caagttgtaa gcaacttcta
     1021 cagttgcatc aaattttgca aagttagttt cttttgcaag tgctacagct tcttctacac
     1081 tgtatgcttt tgtgctgtcg attttctcaa gagcagcacg aagttgtttg ctttttttag
     1141 ccattttcta ttctccttgt aagtggttca atcgattttc atctcccacg tcacttctcg
     1201 aaatgatgaa gtcttgcggg ttttcagtct actatttttc tcttcctacg gccttaagct
     1261 cccttgctgt acccaagtac aagcctcagt cacttgccta gtatgaaaag caactaatag
     1321 actgctgtga gattttctac tgtgttattg attagtcaac aacagtgaat cccatagaac
     1381 gagcagtacc ttcgatcata cgcattgcag actctacgtt tgctgcgttc aaatctggca
     1441 tcttagtttc tgcaatttct tgtacttgtg cacgagtaac tgtagcaact ttagttttat
     1501 taggtgtacc tgatcctttt tcaacacctg cagctttttt caaaagaaca gcagctggtg
     1561 gtgttttcgt aacgaaagta aatgatttat cttcgtaaac tgagataaca actggaatga
     1621 tcataccagc ttggtcagct gtacgagcgt tgaactcttt tgtgaatccc atgatgttga
     1681 taccagcttg accaagagca ggtccaaccg gtggagctgg tgtagcttta ccagcaggga
     1741 tttgcaattt tacaagtttt tcgacttttt tagccatttt taaatcctcc tttgtggttt
     1801 tggcggtaat tagagatttt tacctcccac aagtatgttt ttcacatacc catccattat
     1861 acactattta tctggaattg caagagaaaa gtttattttt tgtactcaat gaaaatcaaa
     1921 gagcaaacta ggaagctagc cgcaggctgc tcaaagtaca gctttgaggt tgcagataaa
     1981 actgacgaag tcagctcaaa acactgtttt gaggttgtga atagaaccga cgcagtcagt
     2041 aggtaaggcg acgctgacgt ggtttgaaga gattttcgaa gagtattaat cgacgtagtc
     2101 tccgccttca aagccatagt attcttccaa actgagacca cttgcagcaa tttctttagc
     2161 ttcttttcct acataacgca ggtgccattc ttcagccata tagcctgttt ccttttcctt
     2221 gcctttgaga taacggacaa caaagccata atcagctgca tgatccaaga gccattgggc
     2281 tgctttttct tctgtcacca aatcaccatc agtcccaatc acatcaaagg ccaagcctgt
     2341 ctggtgttcg ctatagccag gacgggcaga gtaacggtca gctgctgcct ttccatcttg
     2401 gttgacataa tcttgataga gcttggtctg agtttcataa cttctaaaac cactgtaatg
     2461 atcactaata gggaaacctg cctcttgcat cgctttgatg agtttgacca actctgcctt
     2521 ggctgttgga ttttcccctg gattatagtc tttagacaat ggatagtgtt tgttggctac
     2581 gatgatttca tcgtatttcc cttgaatgct gtagtaatca cctttattga ccacttctgc
     2641 tttcttctgg gcagctcctt gagacttact accgactgtt ccatcagctt tggctgtctg
     2701 ttctgtctta gtttctccat cttcattttt tgttttttct tgtgaacaag ctgctagact
     2761 caaggctagc aaagctgtca agactaggta tcttttcttc atttctactc ctttatttct
     2821 aatagtttat ctacttctga cgataaacgt ttgactaatc ttttaatctc atccggtttt
     2881 gcttggcagg tttctgctgt catttcttca aagggaaccc accagactgg tccacgaccc
     2941 ttgagaccat gaccattcat atcacctgtc cgtcgtggaa acagatgcca atgaagatga
     3001 gcatcgccat ttcctagcag ttcgatattc attttctcag cagcaaaggc cttggcaact
     3061 gcctcttgga ctaaactcat ttcttctaga aaacggagtc ttgtctcctt tttcaaatgg
     3121 tgcaattcgc tgacatgctc cttggctaga aagagactat agccttcaaa atactggtgg
     3181 tctccaacca caagatagcc tgtttccaac tctttgacaa agtaaggatt ttcttccttc
     3241 ttgatgaggt caattctctg acaaataagg cacatattag tccaccaacg tgctcttaag
     3301 ataagcatcg accattcgtt ctgttgcgat caccgagtca atatgggtac gctcatagga
     3361 atggctagac tctataccag caccgagaag ggcgtgtttg acttctgccc ctgcagacat
     3421 agccgctgaa gcgtccgaac cataaaatgg atagatatcc agcttaaatg gaatatcttg
     3481 ctctttcgcc aaagccacca aatgttgacg gaagtcatag tgataaggtc cagaagcatc
     3541 cttgacacag atagacactg tatattcgtc tgtttgctgg tcatctccca tggctcccat
     3601 atccacagcc agatattcta ctacctgagc aggaatgtta gagtttgcac cgtgtcccac
     3661 ttcttcaaag actgaaaaag caaaatgagt tgttacgggc aattcaatct tctcttcctt
     3721 ataaatgcga aggagattga gcaaaatcgc cgcactgacc ttgtcatcca aatggcgaga
     3781 cttgataaaa cctgtctctg tcacgacagt tcgtgggtca aaactgataa aatcaccgac
     3841 ctcaatgcca agagcacgag tttctttttc actagttact ttggcgtcca aacgcacttc
     3901 catattgtct tgcgtgcgtt ctgcagttcc tgcatcctta tagacatggc aagaagtttg
     3961 gtggatgagg atggttcctg atactttttc acctgtgcta gccacatgaa tggtacagtt
     4021 ttctccttca atcatgttcc aaggaaagcc accgatacgg tccattttga gacggccgtc
     4081 tggtttgaca gcacggacaa tagcaccaag cgtatctaca tgggcagtca catagcgatg
     4141 ttgctcatca ttttgacctt taatagttac attgacaccg cccttggatg tgcgaaccgg
     4201 ctggtaacca aaaccttcta gagtcttaac taaatagtcc gcaatctcac gagtaaagcc
     4261 tgttggcgac gcaatggctg tcagttcttt gatatattct actgtttgat tcatttcttc
     4321 acctcttttg ctacctatta taacacattt atcatcggat aaaaaaagaa aatcactcct
     4381 gtagacttga ctacaaaagt gattttatac tcaatgaaaa tcaaagagca aactaggaaa
     4441 ctagccgcag gctgtacttg agtacgacaa ggcgacgttg acgcggtttg aatttgattt
     4501 tcgaagagta ttagtgatta ttccatttca aaaacatcgc tttcttgctt gtttggtaga
     4561 accaatgaga gcaagattcc aacaatggct ggcaccaacc atgggagaga tgccttggca
     4621 aaaggaagaa cgctaacaag atttgcaaga aactcaacct taaacgagct tcctagtacg
     4681 cttgcaatgg caataactgt aaccacagca attgtcaact gcatacctgg ttttgaaaga
     4741 gcaacaaatt tgttgacaat gacaatcata acgatagcaa tcgtgattgg gtacaagata
     4801 accagtactg gaattgagta cttgataatc gcatcaagac ccaaattggc aatagcaaat
     4861 ccaatcaagg taaaggctgt cgcataaacc ttgtagctga tttgtgggaa gcgctcatta
     4921 aagaactcag ctgttgacac aatcaaacca acagtcgttg tgaagcaggt tacggtaacc
     4981 atagctgcaa ggaagagttg agctgttgag ccaaagattt cttgagtggc ttgtgacaag
     5041 atgtaaacac ctggtgttcc acccttcatc gcttcagctg gtactgggaa atgatttcca
     5101 agaaaaccta aaccgatgta aagagcgctg aaggcaaggg caacaacgat accaacaacc
     5161 caaatagttg aaatgtattc tttcttactt gaaaatccaa gttgtttcaa ggtttgaact
     5221 gcgattacgc taaaggccac tgaggcaagg gcgtccaagg tattgtaacc ttctaggaaa
     5281 cctgtaccaa aggcagaagc ttgataagca actgaagcag cttgaggact tgttccacca
     5341 tatttgatag ctcccagaac gaccaagata acaatcaaaa ttgcaaagac tggcgttaaa
     5401 atacgtccaa tgcggtctaa gatttttgat ggattaagcg aaatcaaata ggctgccgca
     5461 aaatacagaa ccgtaaatac aatcaagcca agtcctttat ttgcatccga caaaaggggg
     5521 ctaatcccta cttcgtaagc tgttgtagca gtacgtgggg tagcaaagaa tggaccgatt
     5581 gacaagtaaa gaactgagag gtaaagagtc gcaaaccaag gcgctatctt cgttgaaatc
     5641 tcgtagatat accctttagg atttagcgtt ccaataataa gggtcaagac ggcgatacca
     5701 acgcctgaaa agacaaaacc tgcgatggca ggaagaaaat gttctccaga tagagcacct
     5761 agagaaggcg gaaaaatcaa gttccccgca ccaaaaaata ttccaaacag gagcaaacct
     5821 gttagggtac cttttttagc cataaaaatc tccttcatat ttataaaata ctgacctagt
     5881 ataccacgat taggagtctg aaaaaagcat cacgaggcaa atattaaatg ttttcaattt
     5941 tcttaaaaat aagaattatc taaaaataaa actctcctac ccagtccaca ccaagtagga
     6001 gaaatgcaaa attttaaagt tcttcaaagg ctaacatgcc accctggaca ttgataacat
     6061 tataaccttg ttctaacagg aattggcaag cacgcgccga tctcattcca gatttgcaaa
     6121 taatataatg caagcgatct ttgtccaatt aatcataact atcagtcaat tgactaagcg
     6181 gtaggttgtg ggcaccttct aaatgaagag ctgcaaactc atccacttct ctcacatcca
     6241 ctagagaaag ctggtcgttt tggtaaagct ggtaaaatgc gtcaaaggct atttctttca
     6301 ttctttttct tctaatactc ttcgaaaatc tcttcaaacc acgtcagcgt cgccttaccg
     6361 tgcgtatgtt accgacttcg tcagttttat ctgcaacctc aaagctgtac tttgagcagc
     6421 ctacgactag cttcctagtt tgctctttga ttttcattga ctataaaatg gttttaattc
     6481 tttttttcaa atctggcact acttctgcct caaaccaagg atttttggcc atccagattt
     6541 gatttcgtgg tgatgggtga actagcggaa aataggctgg cagatagtct ttatagtgtt
     6601 tcaccctctc cgttaccttc ccactgattt tctcctgtaa atagtaggct tgggcatatt
     6661 gcccaatcaa gagggttaac tgaatatcag gcaattcctg taagagctgc ggatgccatt
     6721 tttctgcaaa acctgtacga ggcggaagat cacccgactt gccatgtcct ggaaagtaga
     6781 aatccatagg caaaacagca aaataacctg aattgtaaaa ggtatcttca tccacaccta
     6841 gccagtcccg caagcggtca ccacttttat ctttccagta aaggcctgct tcttgagttt
     6901 taagtcccgg agcctgaccg atgatattga tgcgagcagt ttttggcgct gcaaagagag
     6961 gctcaatgcc acgctctgta tagctggcat tctgtgaatc cgccatgata gcctgtttga
     7021 ttctttcgat ttgagacatc tactttccct ccaaaaagaa gtccaagcat aagatctcag
     7081 acttctatcg ttttatttga tcaattcgta aatagcttcg gcataaattg ctgctgctcg
     7141 gaaaagatca tccaaggcga taaattcatt ggcttggtgc atggtatcaa tcgagtctgg
     7201 gaacatagca ccgtaggcaa ctccgcgttc tagcaagcga ccaaaggttc caccaccgat
     7261 gacttgttca tgacctttaa ggccagtttg tttttcatag atattcaaca aggtttgcac
     7321 aagtggatct tccattggca catagtgagg cgtgtgaccg tgttcagaca ggctaacaga
     7381 aacaactggc aagttttcaa ggattgactt gatttgttct ggacttgttc cttttggata
     7441 gcggatgttg agggcaatgg tattatcagc acttgtttca tcgaagcgga agacgccggc
     7501 attcatagaa agagcaccca tcttttcatc cacatgagca atcttaagat tttcaccctc
     7561 atgatcgttc aagagaattt tacctgcgat gtcaaggtag tctttggctg gaccagcaaa
     7621 gccaaactgg ctgaggaaga gggcaaggta agttgcgcca ttgacacctg aagcaggcat
     7681 agcaccgtgg gctgatttac caatgatcgt caccttgtat ttgccagctt cttcttggag
     7741 ttctcctcta agtttgtgtt ctgcaacaaa ggcatctagt ttagcttgca agtcagccaa
     7801 gtcacctgaa acgactgctg ttgctgattc tggtaccata ttttcacgta aaccacctgt
     7861 aaagctgtga agacgggcaa cacctgtatt ttctcctgca aagtggaggt attccgtgat
     7921 atttcctttt tcaccattga tgattggaaa ttcagcatct ggtgagaaac cgaaatctgg
     7981 tttggcaagt cctacgtgct caaagtagta gtccatgtct gcccagcctg attcttcgtc
     8041 tgttccaacg atgaagcgaa ctttcttaga agttggaaga cccaattctt tgatgatttt
     8101 caaaccatag taacaagctg ttgtaggacc cttatcgtcc gaagccccgc gcgcataaag
     8161 gcgaccatct ttgatagttg gtgtgtaagg gtctgtgtcc caaccgctac cagcaggcac
     8221 cacatccata tgggcaaaga ttccgagaac ttcttctcca tcaccaaact caaaatgtcc
     8281 tgcatagtta tcaacattct tagttgggta gccatcgcgg tctgcgattt caaggaattt
     8341 ctccaaggct tttactggac caggcccaaa tggatgctgg gcatcagcct tgctgtcatc
     8401 acgttctgaa ttgatttcca aaaggctaaa caagtcagcc aagaggtctt ctttgcgttt
     8461 ttctacttct gctgtaaaat caattgctgt cattttttct tctttctatc ttttctcgat
     8521 gatttcatct actggcaagc ggtagcttgg ttccaatttt tcgtctgtat aacccactgt
     8581 gatcaagagt tctgggcgga aacggtcttc gatttccaaa acttcattaa cttttgattt
     8641 gtcaaaacca aggataatat tggaaccaat tccttggtct gtaagagcga gaaccaagtt
     8701 catagcaacc aaacctgcat tgagtgctag gtagtcgctg acttgttgtt cattgtagcg
     8761 tgcaaattca gctggcaaat ttttcatgaa gtattgaagt tgttcttctg agaaattgtt
     8821 agcaccacca actcgggcaa tcttacgagc gcgtttggca aggtctgtat ctgtaaatag
     8881 ggcaatggtt acaggtgctg atgatacctg ttcaaaattg gaaccataag ctaactttgc
     8941 cagttcagca tttttctcac ataccaccac aaatttccaa ggctggctgt tgtgggcgct
     9001 tggcgccaag gttgcaattt cgatagccgt acgcacatct tttggatcaa caggcttatc
     9061 agtgaaatgc ttggtcgcat gacgtttttt atttaattca agaaatttca taatcgattt
     9121 ccttttctaa ttctactgct tccattctac cataatttga aagagcttgc agggattttt
     9181 catgattaag tttttctatc acagacataa aagatatata ttggtttatc aggaaagttt
     9241 ctgataaact agcaagtact ttacatttga atggagacag tatgaaaaaa tttagcctat
     9301 tactagctat cctaccattt ttggttgcct gtgggaatca agctacaccc aaagagtcca
     9361 gctctcaaaa gacaatcgtc cttgctacag ctggcgatgt gccaccattt gactacgaag
     9421 acaagggcaa tctgacaggc ttcgatatcg aagtcctaaa agcagtggat gaaaaactca
     9481 gcgattacga gattcaattc caaagaactg cttgggagag catcttccca ggacttgatt
     9541 ctggtcacta tcaagcagca gctaataatt tgagttacac aaaagagcgc gctgaaaaat
     9601 acctctactc gcttccaatt tccaacaatc ccctcgtcct tgtcagcaac aagaaaaatc
     9661 ctttgactta tcttgaccag atcgctggta aaacaacaca agaggatacc ggaacttcta
     9721 acgctcaatt catcaataac tggaatcaga aacacactga taatcccgct acaattgatt
     9781 tttctggtga ggatattggt aaacgaatcc tagaccttgc taacggagag tttgatttcc
     9841 tagtttttga caaggtatcc gttcaaaaga ttatcaagga ccgtggttta gacctctcag
     9901 tcgttgattt accttctaca gatagcccca gcaactatat cgttttctca agtgaccaaa
     9961 aagagtttaa agagaaattt gataaagcgc tcaaagaact ctatcaagac ggaacccttg
    10021 aaaaactcag caatacctat ctaggtggtt cttacctccc agatcaatct cagttacaat
    10081 aacactcttc gaaaatctct tcaaaccacg tcagctttat ctgcaacctc aaagctgtgc
    10141 tttgagcaac ctacggctag cttcctagtt tgctctttga ttttcattga gtataagata
    10201 tataaaagcg attggactac caatcgtttt tagtttgcat tggttatttt aggaaactct
    10261 tacaaaaaat caaaaaaatt cttcacatcc tctacatacc tatgcttttc tattaagctt
    10321 gctaatagtt tcagattgtg cctctgatag ttatcttcac gacgtagttc ttcatacatt
    10381 tcgataaagg gaagcccctt ggatttggcc aattctaact cagcctcgta atcataagca
    10441 actttacgaa attggatatt gagtaattcc ccatcttcaa cctctatcac ggcatactgg
    10501 gcacggtgat tttttaacgc ctcccaatta aaatagggca tgccaatcga ccctggattg
    10561 atgatttgtt gccctttact gccgtagcga agcaactgct tgtgaacatg accataaact
    10621 gccacgtccg tttccgcatc tagcagttgg tcaaatttct ctgtatcatt ctcaactagc
    10681 aagtcaccac catagttttt gtcaggtaaa ttatgagaga tagaaaagcg caatccgtca
    10741 atttctttct tttccagcaa aggcaagctt cgtagccaga caatcgttgc aggatccatt
    10801 cgctccatca aaaactgggt catgcgcatt gactggattt ctttcggata ttccaaacca
    10861 tattcgccat ccaagacctc aaggacacga tcatcccaat tgcctcgaac acttgctgtg
    10921 ataggaaggt cctttagcag ggcgactaag tcatttgcgc ctggaccagg aagaaaaata
    10981 tctccctgaa gccaatattc actggcccct tgatttttag catctgcaat cactgcttct
    11041 agcgccgtcg cattgccatg aacatctgat aaaattgcga ttttatggtt cattgtggtt
    11101 tccttccgtt tggtaatcta ctctttcttc tgctacttga ggcaaggctt ctccctgcgg
    11161 gttcaacttt ctttgcacag cctctgcaac gactctaggt accccaactt cgacaatctc
    11221 atccacactg gcttccttga ttttggtcaa agacttgaaa tgcttcataa gattctgctt
    11281 gcgtttaggt cccagaccgt caatcccatc caattgagat gagaaagaat ttttggagcg
    11341 cagttggcgg tggaaagtga tagcaaagcg gtgcacctca tcttggatgc gttggaggag
    11401 gaaaaattcc tgagaattgc gagacaagtc caccacctca agcggatctc caaagagcaa
    11461 ttcatgggtt tggtgcttat cattcttttg cagcccagca attggaatat ccaagcccag
    11521 ttcctcttgg atgacttgct tagcaatatt gacttgacct tgccccccat caatcacaat
    11581 caaatctgga ggagtcaaag cctcacgctg tactcgacca tagcgtctgc gaatgacttc
    11641 tctcatacta gcatagtcgt ccggcccaac aacggttttt atcttgtact tacggtaatc
    11701 ttttttactt ggcctgccgt taacaaagac caccatggct gaaacaggac tggtccccat
    11761 gatgttggag ttatcgaagg actcgatgcg aactggagtc ggaatttgga gcaaacgtcc
    11821 tagattttca atagctcctt gggtcttttc gactgatttt tctagcagat tgaacttctg
    11881 ctctagacta acacgagcat tttttatggc tagattgacc agctgttttt tctctccacg
    11941 ttggggtttg agaatcttgg tatccaccaa agccttgaca gcttcttcgt caatatcctg
    12001 cggaatcagt acctcattgg gaactaggtg agatttttct tgatagaatt gtcccacata
    12061 ggtcaagaaa tcctcatctg gatcattgaa gtaggggaag agattgacat cgcgctcgat
    12121 gagcttgccc tgacgaacaa agaaaacctg cacacacatc cagcccttat ccacatagta
    12181 gccaaagaca tcgcgatttt ggagatcctt agccatgacc cgttgcttgg ttcgaagtgt
    12241 tccaatggcc tgaatcaggt cacggtattc cgctgcccgt tcaaactcca tactttgtgc
    12301 tgttactgcc attttactct tgagatcatc gatgattttg tcatcctgac ctttcagaaa
    12361 atcagacacc tcctgggcca tggacttgaa ataggcctcg tcattcttac agatggtgtg
    12421 ggccatacat tggccgatat ggtagtaaaa acagacctta gagggcgggt tggtacattt
    12481 tcgaaaaggg aaaatcctat ctagaagtcg cttgatttca ttggctgccc ccacatctgg
    12541 ataagggcca aaataaagac caccgtcctt tttgacctga cgagtaatga taagccgtgg
    12601 atagtgctca ttggtgattt tgataaaagg ataagacttg tcatccttga gcatgatatt
    12661 gtacttgggc ttattttcct tgatcaggtt gatttccagc agaagcgcct caatattaga
    12721 ctccgtaaca ataaattcaa aatccacaat ttcagacacc aaggcctctg ttttggtatc
    12781 atgacttcca cggaaataag acctcacgcg attacgcaga tttttagcct ttcctacata
    12841 gataatggtg ccgtttttat ccttgtaaat gtagcaacca gggctggtcg gcaagagctc
    12901 tagttttggt ttaatcaagt tgttcatagt tccattatag caaaaaagta gggaaagata
    12961 gtaccctact cattagtctc atatattttt cggaggtttg caacatcctc ttcctggata
    13021 ccataaaagg aacctgctgg ttgcatgaca gacttctcat ctgtatggtc caagccaatc
    13081 gcatgaccta actcatgttc tgccgtatgg acaaggcgtt cataggaata gccatagtat
    13141 ggattggaca aataataatg attcaaccgc accgttacgg acaagaattg ccctgttaag
    13201 agattagttt gactttccgc ctctcctgcc acaggagtgc ctccgtcgtt catctccgta
    13261 gccgtaatat ccgccttact agactcagtc acgagttcaa agttaaaagc accagtttga
    13321 ttccagttct ggattgcttc taaataagcc tcttgaaagc gtgaatccat ctgcggatcc
    13381 aggtaaatac gagcagaggc ctgtggccac cttccttcag aatgctggtg actatctgtc
    13441 tggcttaact taggcagttg ccctgttttc tcccattggt caatactttg ttgaccaatt
    13501 tttactgacc gttctgcttg ctttagcgct ccttgaaaat ccccgttcag ataccagaga
    13561 agtccgaaag caagcacaca taagagcaca actatccaaa ccagacgcca aaacaaacgc
    13621 cacacaaaag aaaagaaagc ccctatcaaa cgaaaaagcc agcgcatgtc tctccacctt
    13681 tctagcaaat aaaaattatt ttactaaaag aagctaaaag aaatctaaat actgactttt
    13741 tcatcttaac gtcctactat ttttttgagt aactggatag ctttgtattt taaaggagat
    13801 ggatggtaac ggaaagtacc tgctttgcgt acaatatagc cattaaaatt ctgtttaaaa
    13861 cgcaaaacac catcacttcc atcaaaaatc ccttgaatgc ctaggaagtt gtatttaggt
    13921 attccacgtt ttatgctttc caacataaca tatttttgaa gcagtgcagg ggcatagaac
    13981 ttattaaact cagtgtagga accactaaag agataagtcg tttcctgagg catataaaca
    14041 aataaactcc cagctaaaac aatatcttct tctccatatt tttcaatcaa gtctcgcgct
    14101 tctgcttttc gaacttcaaa cgtttcaaat tgactagaat attctctcag ttgattttgt
    14161 tttttctcag aatgaggatt tttactcaaa tcaagtcgca acttgtccaa gttttcttct
    14221 agtttatttt gttcaccttg caatttgctc atatagtccg aaaaatttaa gcttgctatg
    14281 agaaactccg cttgttctcc aaaagtatca taaaaatgct cataatattc taaactttta
    14341 tcactatatt ctctacgttc agaggtttct tttgttatat tcttaaaaat cgatagttct
    14401 tcacgtttta actttttcaa tcgaatgcca aaggtttcag cctttttcac caagggttta
    14461 ccctttttgc taaaactttt aagcaaactc ttttcagtta attcagttaa atctttatag
    14521 tataaccaat ctggttctcc acctgggtaa cctgttgtta agccatcaaa ttgataacct
    14581 aaatcagtca aaccttgaat aatacttttt ttctcagcat ctattggatt accttggcta
    14641 tcaaaagttt gataagtttc ataaggtttt acaagcaact ctaatacacc attttgcttg
    14701 gcatattctt ttaactctgc ataaaaaact ggaagagcat cttgttgggt ataaatcggc
    14761 cccgaattga gttccatatg cagaccaccc agcatgggca agctataaac cagagctgca
    14821 acttgaattt ctccttcttg tttcaaagca agataaacaa ttcgagcccc tcttttttct
    14881 agcaaatccc ccatctggac agattgcata aaggaacgag aagaaacctg atcagaataa
    14941 gtctgaaact cttctttcgt gagtgttgtt agtgccatat acttactttc tatgtttttt
    15001 tcttaatgtt ttacggaaat caagagcaag tcttaacaga ggatagagag gatgagtggg
    15061 cattgtaaat tcacccaagt attcttcaat cgttggatta aatttttcct taaaatgata
    15121 aagtccacca ttgagagagt tttcaacacc acctaaattt tgccagacca tacctcgctc
    15181 aaaggcatag cgagccgttt cataccatgt taaaattggt gcattgtaac gtttaaaatc
    15241 atcatccata ccagcatata tattgacaga ggtagtacca aattccaaac tcaaagtagc
    15301 cgctaaagga actctcgctt gacctacatc tatatattcc tgcaagaagg tcaattcctc
    15361 taacaaacgt tctttttcct tcttctgcgc ttctactttt gaagttcgag tcgactcagt
    15421 aaaggtctct tccaaggctc tatttttcgc taactgttct tctaattctt gcgaacgttt
    15481 agaaacatcc aaggtggcca aggtgatata ggccttgtcc ttaaaattat ctaacaattt
    15541 tttataatag gcttcattcc tcaaatgaat ctcttttcgc ttctcagttt ttttcatcaa
    15601 ctccgaaaat gaatctaata gttccagtcc accatattga atctcaagcc ctttgtttcg
    15661 tgctgttcga atagcctgtt ttgttgactt ggaaagttta tcttcttcaa aattttcctt
    15721 gtatattttc gcctgaatac gaggttgaat ggtgtctccc atttcctccg tttttcctga
    15781 ccaccttact cccatttgtt gcaaactatc aataatagcc agattttcag gaaattctgt
    15841 cttttcctga ttgattaaac tttgagatag gcaaatactt gggtcaaaag tcacaaaaac
    15901 cgctctctta ctgcgagcat aggacttaat agactgaatg gcaaaattca agagttcttt
    15961 atccccataa tccaatatag gtcctcttgg gatgtaaaac attttatagc ctagcggaag
    16021 agttctaatc aaaatactag ctgtcgccag taatttttct tccctgtaaa caccaaactt
    16081 ctcatgttgc caattagact taacttcctc ccaagcacta ctttgtaata cattggctaa
    16141 ttcatgttct ttgacaaact gatcatattc taatgtggga atgccaattt gataacggta
    16201 catttcctac tctctttcct ccagtatttt attactattc tactactttc gtttgaaaag
    16261 tctagtgaaa acatatttta ttttagttct actcccccta tgctgaagtt caaactaagt
    16321 cagtctctct tctaatttga aatcaattgg tagggttgtt aaaaaaactt ccaattgttt
    16381 ttcccagtag taccactcgt gagttccagc actatggcta taggtcacat caaaacctag
    16441 ttttttgaga tttttcactg cgagattatt ggcttcgtac aagaaatcct gttcgccaca
    16501 ccacgcccaa agtttggtct ttttatccga ttttttagcc agactttcaa gagaataggg
    16561 actagttgtc cagtctctaa tctctccaaa aacacctctc cagtaggctg gacttcccag
    16621 attttgactt tcaggagaaa agttttgaaa gctgagggca cctgaaaaac tagctgcatg
    16681 agaaaaacga tttgtcgtaa gagccagttt gaagcagccg tagcctccca tagaaagacc
    16741 agcgataaag gtcttttcac gcttgctagt catattaggg aagaagcgtt tcagaacctg
    16801 tggcaattcc tctgctagag ccgtgtagta gtcaaaacca tactgggtat cggtgtacca
    16861 accattgctg gtattgggca taacaacgat gagattagtt cctcgaagca agcgttctac
    16921 attggtccgc ttaagccaac tattatgatt tccagacatc ccgtgcaaaa ggtacaagac
    16981 gggaatatct tcacactctg gttcttccac tcgattggca tcagggtaga ggacattcac
    17041 cccccactcc atatccaata cttgtgagta atactcgatt ttcattactg ccatgatttt
    17101 ctccttctat ttttctataa gaaaaagccg aaactcgact ttctctctgt ttatttcaac
    17161 gattattttg cttccatgac tactggtaaa atagctggac gacgcttggt ttggtcaaag
    17221 agatacttgg tcaaattatc tcgaaccttc cctttaagat ctgcccagtc aaagtcatct
    17281 ccttgaagat aatcttctac cgtttggtta atcaattctg aactttcacg gagaatatcg
    17341 cgactcttct tgagataaac aaatccacgc gtgtgaacac gagccctagc cacaattttc
    17401 ttctcacgac ggttgactgt aatagccacg atgaaaattc catcctctga caagacctta
    17461 cggtcacgaa gaacaacatt tccaacatca ccaatggcat tcccatcaat caagatatct
    17521 cctgctgaaa ccgatccagc tggaacaaag tctccattct cgtaagccat ggtcgtcccc
    17581 tttttaggaa tgaaaatgcg ttctggcaac atcccaactg ccatggcagc cttagcgtga
    17641 gcatccaact cacgatactc cccttggaca gggaagaggt acttaggttg caaaagattg
    17701 atcatcagct gcaaatcacg cacatttccg tgccctgata catgtaaact ttgggtaatc
    17761 aatttgacaa ccccacctgc ctgataaatc atattttcca cacgcgcaac aaaggcttct
    17821 ttagcaatag acggagccgt agcaatatag accaggtccc catccttgat ttctacataa
    17881 cgatggcgac caatcgacat cttacgaagt ccattgatag gttcacccat acgacctgtc
    17941 tcaagaataa tcaactcatg gtcttcaaag cgagacatat ctttaggctt aatcaagaga
    18001 atttcgttgg ctaaagacaa cttcttaaga cgaatcgctg tgcggacgat attttcaata
    18061 tcaaatcctg tcaagacgat acgtcgacct gttttatccg cagcgtcaaa aatctgctga
    18121 atacgagaaa gattactgga aacagctgca acgatgatac gaccttccca gtcagcaata
    18181 gtttgggtaa tttcatccct aacttcactt tcactagcca cctgaatatt gctgtctgca
    18241 ttggccgaat cactgaggag agccaggacg ccgtcacgac caatctctgc caaacgagca
    18301 aaatcagttg cataagattc actagccgtt tggtcaaatt tgaagtcacc tgtataaacg
    18361 atgcttcctt ccgatgtctt caagacaatt cccagactct ctggaacgga gtaagtcgta
    18421 gggaagaagg aaaccactgt cccaccaaaa tcaatctccg tattctcatc aatgacatgg
    18481 aaatcattaa atttcttaac ggcatcattt cctttgacaa agagctttgc caactcaatg
    18541 gtcaactcag acccaaatac aggaacttta gcctctgcca agagataagg tagagcacca
    18601 atggcatccg catgcccgtg ggtcaagaaa accccagcaa tacggtcgct attttcaaaa
    18661 aggtaatcca tgtttggaat caccacatcg acccctaatt gttcattttc aggatatttt
    18721 aaccctacat tcaaaacaaa aatggactct ccaatttcag caatgtacat attttttcca
    18781 ttctcacgca caccaccaag tgttgttaaa ctgatattac tcatttttcc tccaaaatct
    18841 tagtttatct tgattatagg gcaattctac cctcttattc taaaagttct gatactttca
    18901 gccgaatctt ttcctaccat tataccattt ttttgatgat tttcaaaatg aaggtctgtt
    18961 ttcaaaaaag agttgatttc ccaactcttt cctaacttct actctttttc catcaaaaaa
    19021 tcataaattt cttgcaccgt acccagcgcc atcatttctt ggtctttaac ggtttgtttg
    19081 agattttggt aaatttgact ttctcctatc tcccgctttg gcgcctcttt attgactgtc
    19141 atcacaccat cttttatcgt cacaaagcct agttcttcaa atacttgaat catcttgacc
    19201 agcaagattt gttgaatatt aagatatgca gccaaatctt tcagcttgta gcgaatatca
    19261 aactctggga actggtaaat agtcttgtac aatttagcaa actgatctct agtcccataa
    19321 cctgtcagat aataggtctt gtcaatatca tttttgaaat agacagcaga gaaatgctgt
    19381 tcctgaaaaa tggtcttcag ctgagtaata tcctctggaa tgttttttac gacaacagct
    19441 tcactagccg caagatttgg cagttctcca ggaaaatcca agactggaac accttctggc
    19501 aagactgcat tttttccacg aatgttaaaa agttgaacac cttccactcg cgcatccacc
    19561 atcatcaact ggagggcagt ttggccattc cattggttga cagacaattt aaccgctaac
    19621 tctagattct tggtttgaga aaactctgtc gcccatctgc cttgaccaaa ggctaccact
    19681 tcaaaactcg cctcaccctt ggaaattttc agctttagat gggcattacc tgcccccata
    19741 gtacgagcac tttcgacctg aaaattcttg atataaaaaa taggtttctg attatccatt
    19801 ccaaaaggag ctaaacgttc aaaacttttg accgtttcca agctaagtgc ctccaaatcc
    19861 aactcttcat ctaggtttaa cttattcttg ccaccagcat ctgcaccttt ttcacgaaca
    19921 taatcttcca aaacctgaga taaatctgag agttgctcaa cttccagcgt catacccgct
    19981 gcacctgcat gacctccaaa ggcgatgaag aggtctcgat ggggatccag agcttcaaaa
    20041 atatcgaccg cttccacact acgagcactg cccttggcac gaccgtcttc tatattaaga
    20101 acaatgactg tctgtcccaa ttcttccaat aaacgaccag ccacgattcc tagaacccca
    20161 ggattccagc cttccttggc caagacctga accttcttct caggatccac catggtcttg
    20221 gcttcttcat agatagactg aacgatttcc ttgcgctctt cgtttttctg gtgaatcata
    20281 agggcaatct catgcgcttc ctcatcatca aatccagtca acaaatcaat ggcaggattg
    20341 ggatcatcca agcgacccaa ggcattcaaa cgaggagcaa tctggaaacc aaccgtttct
    20401 tctgttactt cgttggcagc aatcccagcc atgtccagca tttcttgcag accaatgcgc
    20461 tgggtatgac ccaacatttc cagaccatat tgaactaaga tacgattttc atccgtcaga
    20521 ctcaccatat ctgcaatagt tccaatagcg accaaatcaa gcaattccac ttgcacttct
    20581 tctaacaggg cacaagccaa cttgaaagca actccacaac cagccaaata tttaaaagga
    20641 taatccgcat ctggatgttc aggatggaca atagcataag catctggcag ggtttcaggc
    20701 atggaatgat ggtctgtcac aatgacatct actcccatag actgagccaa tgcaatagcc
    20761 tcatgaccag caaccccatt gtccaccgtc acaatcaagg aaattccttc ttgctcgata
    20821 aagtatttat aaacactagc attagggcca tagccatcgg taaaacgatt tggcaggtaa
    20881 actcggcact cagcaccaag ttgttccaaa ctttccttca caatagaagc cgaagtcatg
    20941 ccatccgcat catagtctcc ataaacgaga atattttccc cttcttcaat agcctgacga
    21001 atccgctcca ctgccttgtc catatcatgg agcagataag catcatgtaa gtcctccaag
    21061 gaaggttcta aaaacttctt cagactttct tggtcctgaa tccctctctc aaacaataac
    21121 cgagccacct caggacccag tccagccttc ttggctatct ttgtaaaatc cgcatcttct
    21181 acctgcaggg caaactgcca ttcataagta ggtgttatca aaaagacatc cactctttct
    21241 aagaattcta ttgcttcatt ataacatgat ttagacgttt tctctatcca gattgctttt
    21301 ttataacatt ttatagtata ttgaaactag aatagtacac ctctacttct aaaatattgt
    21361 tagaaatcga tttgactgtc ccgatcgatt tttcctgttc ttatttcact atagtgaatt
    21421 ttttcagata tgatttgaca agaacattct tttattgcag aatcgtgtta taaaatctaa
    21481 cataaaggag ttctctcatg gttttagtag aatttaaaaa cgtcgaaaaa tattacggag
    21541 actaccacgc actccgcaac atcaatctcc gttttgaaaa aggacaagtt gttgtcctgc
    21601 ttggaccttc tggctctggg aagtccactc ttatccgtac gatcaatggt ttagaggctg
    21661 ttgacaaagg aagtctccta gtcaatgggc accaagttgc tggtgccagc cagaaagatt
    21721 tggtacctct tcgcaaggaa gtcggcatgg tttttcaaca ttttaacctt tatccacaca
    21781 aaacagtgtt agaaaacgtg acacttgcgc ccattaaagt tctaggaatt gataaaaaag
    21841 aagctgaaaa aaccgccaaa aaatatctgg aatttgtaaa tatgtgggac aagaaagatt
    21901 cctatcccgc catgctatct ggtggacaaa aacagcggat cgccatcgct cgtggtcttg
    21961 ctatgcatcc ggaactcctc ctctttgatg aaccaacatc tgctcttgat cctgagacta
    22021 tcggagatgt tctagcagtt atgcagaaac tggcgcgtga taggatgaac atgatcatcg
    22081 ttacccacga aatgggcttt gctcgagagg ttgcggaccg cattatcttt atggccgacg
    22141 gagaagtttt agtagatacg acagatgtcg ataacttttt tgacaatcca agcgaacctc
    22201 gtgcccaaca attcctcagc aaaattatca accacgaaag tgacaaagtc aaataaggag
    22261 gcgcctatga aaaaaaattc tttttatcag cattattgat tagccttttc ggccttgctg
    22321 ctgccaaacc agtccaggct gatacaagta tcgcagacat tcaaaaaaga ggcgaactgg
    22381 ttgtcggtgt caaacaagac gttcccaatt ttggttacaa agatcccaag accggtactt
    22441 attctggtat cgaaaccgac ttggccaaga tggtagctga tgaactcaag gtcaagattc
    22501 gctatgtgcc ggttacagca caaacccgcg gcccccttct agacaatgaa caggtcgata
    22561 tggatatcgc gacctttacc atcacggacg aacgcaaaaa actctacaac tttaccagtc
    22621 cctactacac agacgcttct ggatttttgg tcaataaatc tgccaaaatc aaaaagattg
    22681 aggacctaaa cggcaaaacc atcggagtcg cccaaggttc tatcacccaa cgcctaatta
    22741 ctgaactggg taaaaagaaa ggtctgaagt ttaaattcgt cgaacttggt tcctacccag
    22801 aattgattac ttccctgcac gctcatcgta tcgatgcctt ttccgttgac cgctctattc
    22861 tatctggcta cactaataaa cggacagcac tactagatga tagtttcaag ccatctgact
    22921 acggtattgt taccaagaaa tcaaatacag agctcaacga ctatcttgat aacttggtta
    22981 ctaaatggag caaggatggt agtttgcaga aactttatga ccgttacaag ctcaaaccat
    23041 ctagtcatac cgcagattaa ggaggatacc ctatgacaga tttatcatct tggacagcct
    23101 attttcagga ttttggacaa tttttcaatg gtttcctctt tactcttgcc ctagcgattg
    23161 gatcctttat cctcgccatg gtcttaggaa tcttctttgg tgccatgtca accagtaaac
    23221 gtccaatttt acgcatttta gctcgcatct ttgtggaatt ttaccaaaat actcctctct
    23281 tggtgcagtt tgttattgtt ttttatggcc tacctcttat cagtgaccat atcatcatga
    23341 ttccaattta ttggacagcc gttctctgcg tgggactcta tcacggcgct tatatcgctg
    23401 aggttattcg ttcagggatt cagtctattc ctagtggtca gatggaggcc gccttgtcgc
    23461 aaggttttac ctatatcagt gccatgcgct tgattacctt gcctcaggcc ttccgcatca
    23521 ttctccctcc attgaccaac caaattgtta acctcatcaa gaacacctct actgtagcta
    23581 tcatctctgg agtagacttg atgtttgtga ctaagtcttg gtcggctctc aacggaaact
    23641 atatcccagc ctttttaggt gctactcttc tctactttgc cctatgcttc cctgttgccc
    23701 agtttggtcg caagatggag caagccaaca aaaaagccta ttcactttag gaggttacta
    23761 tggaatctat tttagaagtt ttaaccccag ataacctagt ctttatcttt aaaggatttg
    23821 gcttgaccct ctatatttct ctgattgcca tcatcctctc tactatcatc ggtacggtgc
    23881 tagctgtcat gagaaatggc aaaaatcctg tcttgcgaat catctccagc atttatatcg
    23941 aatttgtgcg caacgttccc aaccttctct ggatttttac tatctttttg gtgttcaaaa
    24001 tgaaatccac accagcaggt attacagcct ttactctctt tacatcagca gccttggctg
    24061 agattattcg aggcggtctc aatgccgtag acaagggaca atacgaagca ggaatgtcac
    24121 aaggcttcac ctcagcccaa atcctctact acatcattct cccacaagcc atccgcaaaa
    24181 tgctaccagc catcatttct cagtttgtta ccgtgattaa ggataccagc ctcctctact
    24241 ctgttatcgc cctacaagaa ctctttggag ccagccaaat tctcatgggc cgttatttcg
    24301 aaccagagca ggtcttcagt ctttgcatcc tgattgccct catctacttc agctttaacc
    24361 tagcaatttc tagcctgtct catatgctag ccaaacgttg gcaacaagct gcagaataat
    24421 actcttcgaa aatctcttca aaccacgtta gctctatctg caacctcaaa acagtgtttt
    24481 gagcaacttg cggctagctt cctagtttgc tctttgattt tcattgagta taaacagcca
    24541 ctctccagtt caattggaga gttttttgat gagagtagat atttatagac ctcaatttga
    24601 caccatacat tttctatgat aaaatgtaac aaatttattt acaacaaatc gaaatctaaa
    24661 tgaaatggaa ttttctatga aatcagtcaa aggactactc tttatcatag ctagttttat
    24721 cttgactctt ttgacttgga tgaacacttc tccccaattc atgattccag gactagcttt
    24781 aacaagccta tctctgactt ttatcctagc cactcgtctc ccactactag aaagctggtt
    24841 tcacagtttg gagaaggtct acaccgtcca caaattcaca gcctttctct caatcatcct
    24901 actaatcttt cataacttta gtatgggcgg tttgtggggc tctcgcttag ctgctcaatt
    24961 tggcaatctt gccatctata tctttgccag catcatcctt gtcgcctatt taggcaaata
    25021 catccaatac gaagcttggc gatggattca ccgcctggtt tacctagcct atattttagg
    25081 actctttcac atctacatga taatgggcaa tcgtctcctt acatttaatc ttctaagttt
    25141 tcttgttggt agctatgccc ttttaggctt actagctggt ttttatatca tttttctata
    25201 tcaaaagatt tccttcccct atctagggaa aattacccat ctcaaacgct taaatcacga
    25261 tactagagaa attcaaatcc atcttagcag acctttcaac tatcaatcag gacaatttgc
    25321 ctttctaaag attttccaag aaggctttga aagtgctccg catccctttt ctatctcagg
    25381 aggtcatggt caaactcttt actttactgt taaaaattca ggcgaccata ccaagaatat
    25441 ctatgataat cttcaagccg gcagcaaagt aaccctagac agagcttacg gacacatgat
    25501 catagaagaa ggacgagaaa atcaggtttg gattgctgga ggtattggga tcaccccctt
    25561 catctcttac atccgtgaac atcctatttt agataaacag gttcacttct actatagctt
    25621 ccgtggagat gaaaatgcag tctacctaga tttactccgt aactatgctc agaaaaatcc
    25681 taattttgaa ctccatctaa tcgacagtac aaaagacggc tatcttaatt ttgaacaaaa
    25741 agaagtgccc gaacatgcaa ccgtctatat gtgtggtcct atttctatga tgaaggcact
    25801 tgccaaacag attaagaaac aaaatccaaa aacagagctt atttacgaag gattcaagtt
    25861 caaataagta atcatactct cctgggattt cccaggagag ttttttatag ccaaatgaaa
    25921 taagacagga acgatggaat tttggcattc aaatcaattg ataagaatgt tttatagtgg
    25981 agtgaaatag gatacgaaca aattgattag gaaaatcaaa tgaatttata gaaatctttt
    26041 agcagttatg ttgtcctatt ctagtttcaa aacgctatag aagcagcatt gtgctagtct
    26101 agattcagtt tactatacta aaacgagtag cttgaaatca aaaaacccac cctcacaggc
    26161 aggttttatc tgtattattc agctagatta tgctttacct tctgaaccga atacgtcgat
    26221 acgttcttca accgatgctt ggatagcttt tacaccgtca gccaagaatt tacgtgggtc
    26281 gaagagtttt ttcttgtcgt attctgcttc gtttgcttcg tagtcacgag caaatttacg
    26341 agttgcgtta gcgaatgcga tttggcattc tgtgttaacg ttaactttgg caacaccaag
    26401 tttgatagct gcttggattt gctcatcagg aatacctgat ccaccgtgca atacgattgg
    26461 gaatcctgga agagcttctg tcaatttttg caagtggtca aggtctagac cttcccagtt
    26521 tactgggtaa gggccgtgga tgttaccgat accagctgcc aagaagtcga taccagtttc
    26581 aaccattgct ttagcgtctt cgattggagc caattcacct ttaccgatga ttccgtcttc
    26641 ttcaccaccg atagtaccaa cttcagcttc tactgagata cctttagcgt gtgctttttc
    26701 aacaacttct ttagccaatt taaggttttc ttcaactgga aggtgtgaac cgtcaaacat
    26761 gattgaagta taaccaactt cgatacactc aagtgcatct tcgtagtgac cgtggtcaag
    26821 gtggatagct actggtacag tgatacccat tgattcaaca aggttagcga tcaagttgcg
    26881 agcaactttg taaccaccca tgtatttagc agcacccatt gaagtttgga tcaaaactgg
    26941 agctttttta gcttctgctg cgcgcaagat agcttgagtc cactcaaggt tgtttgtgtt
    27001 aaatccacca actgcataac cgttgtcacg ggctgcttgg acaaattttt ctgctgaaac
    27061 gattgccatt ttatcaggcc tcctgtatat ttttatgggt catcccattt acattgttca
    27121 ttttatcact ttttgccaaa aaaatctagt ttttcccgca gtttcgattg attttcttct
    27181 aactccatct atgtaaaccc tttctcccta gtcttggacg acttttggaa aatctataaa
    27241 gaaggttaaa cgattctcct ccatctcgaa acgataagct aatttttcat gttctaatag
    27301 actcttaacc acaaagagcc ccataccaga ccccttgacc ttgcgactag cattgtcaga
    27361 aaaagactgg gctagttttt cttgttcctc tgagctacag ctattttcga taaaaagttc
    27421 tccttctctt tctccaattc gaactaagcc acctggaaca gagtgcttaa tggcattgct
    27481 gatgagatta gaaagaatca acttcataac tgatgggttt agataagcct gctgatgggt
    27541 caaactattg tctatctgga gctctctttc ctttgctagc aaggcataat ctttgaccag
    27601 attttgcgtc atctggagga ggtcaattgt ttccctatca tctcgcaatt cctgcacaga
    27661 agagagggaa agtatctgca gaacatggtg attgagttca tccacaatcc ccaaggcaac
    27721 tcccagatac tggtctctat ccttataacg accgatattc tctctcatat tttcgattag
    27781 gattttcaaa ctagccagcg gtgttttcaa ttcatgagaa gctcctcgta ggaattcgac
    27841 cttcatcttc tccagctgga gaatggcttc attcttttca tgcaagtccg caataacagt
    27901 caagagatgc tggtagaggc tattgatttg ttccttgaga ttacctatct catccttaga
    27961 atccacgcgc aatcgcactt gggaatccag gtccatcatc cgacgggtca cccgcttgat
    28021 ttccaaaatc ggtgcaacaa tagtccgagc gtagatgtag gccaccaaga gggaaatcag
    28081 aaaggaggcc agcaaggtat agggaagaaa ctggagactg atttgctccg cttccttttg
    28141 taaatccatg gaagctagaa actggagaat catagtacca ccgtcttgcg ttttcacctc
    28201 gcgctcctca ataaagagag aggttgtctg gcggtctgtg tccagaggaa gactgtcctt
    28261 gacttctaac ttgtcctcgg tcatctcacc tttgacggtc cccttgatat cactagtctg
    28321 ggaatacaag tctaacactt gctcgatact ctgcctatct ttcccttcta gggactgggc
    28381 aatggctgtt gccttttgac caatggtttc ctgacgatga ctcagataag tcgaaggaaa
    28441 aagaaaataa atagctaaat gaaggcagat aaccagaaca ctaaatatcg agaaggtata
    28501 gataaatatc tttgcaaata aacctgttcg tttcattttc gctccaattt ataaccaaca
    28561 ttgcgcacag tgaggataca atccaagtct agctttttcc gcaattcctt gatataaaca
    28621 tcaataacac ggtcaaaggg aacctcatct gtcgctttcc agacggcatc gataatctga
    28681 gatcgagtca aggcccggcc ttcatttttc actagatagt ccagaatttc caactctttg
    28741 gcattgatag gcacttcttg acctgcgagg cttgcactgt agctttcaaa gtccaccttg
    28801 gtatccttgt aagaaaagat tcgtcctgta tcgtagtagc gcttgaaaat cgcgtccacc
    28861 ctcactttta aaagggagag ggagaaaggt ttttccagat agccatctgc caaagaggca
    28921 aaggcactca tcttgtattc ctcatcttga aaagctgtca acatcaagac aggaacctga
    28981 ctggttttac gaatctcagc taggacttct aagccgttga gcttgggcat ctggatatcc
    29041 agtaaaacca gggccacctc atagctagaa aattgctcca gagcttcctg accgtccgct
    29101 gcctcaatag tttcatagcc acaatccgtc aaataatcac tgaccccctc acggatcatc
    29161 tcttcatctt ctacaattaa aattttcata ctttaactgc tctctatttt ttatttttct
    29221 tagaataaat acctacccta ttttctatta tagtctcttg ctggcctttt gtctgcaagc
    29281 aactgaccac tagataaaac gttgtgaaat tcctttctca taaattccat aactttagta
    29341 tattatattt aagcactaaa gtacaaagaa agcaactgaa agcaatgatt ttcaccactg
    29401 ctttcggatt tattttgaat tgttaaatag ccattcctat ccactattct tgaatagaaa
    29461 cacaagatgc aatctttatt ctagactcat tttttcaaat ttattcacca tccagcaaga
    29521 gctcttttgg ttgttttcta aggagattgc ttgaagcaag cgccataacg agaaccacta
    29581 gaaccaaggc aaggacaaaa atgatgataa agtctgatgt ctgaatggaa atgtctaggc
    29641 tcgacaaggt cttgctaaag ccatctactt ctgcaccacc accaaggtta gaggcttgag
    29701 ccgccttact agcctgtttg gcaacacctg aagtcacatt ggcaaggaca gtgtttccaa
    29761 ttgcacgggc agtgtaatta gctaggaagt aagcagaaac tagagcaggg atagcaatca
    29821 agatagattc ggtgatgaat tgacccaaga tacttgcctg cttgaggccg atagagagga
    29881 gaattcccac ttccttgcga cgggcgttga tccaaaggct gagcaagagg gcaaggagga
    29941 gaactgagaa gctcaagcta ccccagaaga ggaggttggc catcttgtac ataccagaga
    30001 tagattgctc aagagctggg tagttagagg agctcttgac gagtgtgtag ctcttccagt
    30061 tgataccact gatgccattc aactctttca taacatcatc caagttcttg tctgctgtta
    30121 caaagaaggt tgcgtcccca taaatggctg tgtcttctgt gtatccataa agttttgcag
    30181 cagtgtgaat gtctgtaata gctgtgtttt cgtaaagttc ttgtgagtag gttactgctg
    30241 acttattatg accatcaaag agtcccttga ttgtcacttc aactgtttcc ttggctcctt
    30301 tttcattatc tgcatcgtag atattagagt ccagtttaac cttgtcccct actttccagc
    30361 cgtgtttggc tgccaagtcc ttgtgcaaga ggattttatc cttgtcgtcg ttggttaagt
    30421 gctctccttc gactagttta taagaaccag agacaaactt gtcttcttta gaggagtcat
    30481 tgacacctgt aatcatcaag ctacttccaa aacgcttggc acgatcagca gtgagattct
    30541 tcttggtttc tggcgtttca atcaggtcat atccagtcaa atctccgata gcgttgatac
    30601 gtttgacata agactcaatg gccttgtttt cggtgatttt tttgatgtct tcacccttga
    30661 tattcccagc accacgaggc gttccttggt tgacgcgacg attgatttgc atggagaagc
    30721 tattggtgat atttttaaag gtctcctgag aagccttggc agtagctccc ttgattgaca
    30781 agccgaccaa actcaagctc gccatgagga gaataatcag gaagatgaca atcgatttga
    30841 aaaacttcct tgtaacatag gcaaatgcgt tgtgtaacat agattccctt tctagatttt
    30901 gttttaatca ttctattaaa ataagctcaa attatttact agtattgcgc gtttcagtca
    30961 gtttcttatc ctttaattca agtgtaatat ctgacgcttg tgccacttct ttactgtgag
    31021 ttacgacaat cacacattta cctgttttct gggcaagtga tttgagtagt tcgacaatat
    31081 ctccagcagt tttaggatcc agatttcctg ttggctcatc agctagaata actggagctt
    31141 ctgagaccaa actgcgagca atggcaacac gttgctgttg accacctgat aactggagaa
    31201 cattccgctt gatctggctt tcatccaaac caagctcaag aagtgtattc ttgcttgcct
    31261 ttttgttgac caatcggata ttttccagcg gagaaagata atctatcaag ttataatttt
    31321 gaaagaccag ggaaatatgg tgcatgcgat ggtaagaata gcccttctta cgaatatcct
    31381 ctccttgaaa aaggatagaa ccttcaacag gactatctag accagcaagt agggacaaga
    31441 gtgtggattt tcctgctcct gactccccaa taatactgta aaattttccg ggttcaaaat
    31501 tataattgat ctgatatagg actgcttcag cagtattctt ataacggtag gtaacatctt
    31561 gtaattgtaa taaagtcatg atttctcctt cttaactaat agatgataaa atttctttcg
    31621 gtgattttct aaataagaat aggaaacaaa gggctacaga taagcaacta agcagaacta
    31681 gaaaaacata ggattctgca aaagataaga tgctagttga taaactgctt gctttggcta
    31741 gtgtatcttg taagcttgcc tgatctccac ttgctagtag agtttggagt aggtaagttg
    31801 tgattgcgtt tcctgcaaca aatgctggaa gcaaagctcc aagagatacc aaaactacct
    31861 ctaaacagaa ttgtaggaag atcgagctct tgccttttcc aagtgcaagt aaaatcccca
    31921 cttcatagac ccgttctctc aaccagagag acaaaaccag aattaaggct ccagctcctg
    31981 ctatcaacat cccataaagg aagatggtca ggaaggtttg gaaagttgca actgagtctt
    32041 tgatttgttc aaaagccttg ttttcctttt cgacttggta gccttgattt tccaaggcca
    32101 agttttctac ctgcttcatg agtccgtcca tttccttagg attttctaca tagaagcgtg
    32161 ctgcactgac ttgagcttca ctattgccca aaagggtttg gctactttca tagtctgtaa
    32221 agacttgatt ttcactgaag tcagaagaca agcctgtgaa tttctcttgt tttttaccag
    32281 aaaagatgcc gataatctca aactctactg tttgtccttt tccagattca gactgaccag
    32341 catccaagcc aatcttgtca tgaagcgaaa gaccgttctt cttagccaat tcttcgtgga
    32401 taaggatttt cttggaatcc cctttttgaa ggtgtcgccc ttcttttaga ttgaaagccg
    32461 aactggtaaa ggttacatcc ttggatgaat cctcaagagc cgttaagcta accaagttat
    32521 tgtctgcagc tgataaatca tcacgctcca cgctctgctc gccagtcact gcttccttgt
    32581 cttttagttt tgcgaccgtc tcaagttcag gagagacatt ttccagcccc ttaatcttgc
    32641 ttacagatgc taggtctgac aacttgaatg tctgaccatt ctctatcttc ttaatagaaa
    32701 aagatgtatt gagtgattta taaagattgc tttctactgt tttgttggac ttcatcagag
    32761 tcaaacaggc tgaaattccg gccaataaga ccaataaaat cagaaataaa ataaaacttc
    32821 tcagtcgctt tctgctgaca taagcccaag atctttggat tggattcatt tgtcacctcc
    32881 atatttgtaa gactattata aaacccaaat atgaaatgtt tatgaaatac gaaaaaaaaa
    32941 tatcgagtag gggataatct ctagcccctc tcacaccacc
//
LOCUS       DAVKQB010000024        31665 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKQB010000024 DAVKQB010000000
VERSION     DAVKQB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.024"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..183)
                     /locus_tag="VW528_001088"
     CDS             complement(<1..183)
                     /locus_tag="VW528_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9911917.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADFISKSQLSRFERGENEISL
                     STFFELLSNINVSIENF"
     gene            complement(196..969)
                     /locus_tag="VW528_001089"
     CDS             complement(196..969)
                     /locus_tag="VW528_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9911918.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGTGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLAS
                     LFKDEVNQDLENRTFTLQNGKRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
     gene            complement(1415..2170)
                     /locus_tag="VW528_001090"
                     /pseudo
     CDS             complement(1415..2170)
                     /locus_tag="VW528_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(2308..3930)
                     /gene="groL"
                     /locus_tag="VW528_001091"
     CDS             complement(2308..3930)
                     /gene="groL"
                     /locus_tag="VW528_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9911919.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(3946..4230)
                     /gene="groES"
                     /locus_tag="VW528_001092"
     CDS             complement(3946..4230)
                     /gene="groES"
                     /locus_tag="VW528_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9911920.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(4500..4895)
                     /locus_tag="VW528_001093"
     CDS             complement(4500..4895)
                     /locus_tag="VW528_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9911921.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(4973..5734)
                     /locus_tag="VW528_001094"
     CDS             complement(4973..5734)
                     /locus_tag="VW528_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9911922.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(5767..6393)
                     /locus_tag="VW528_001095"
     CDS             complement(5767..6393)
                     /locus_tag="VW528_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9911923.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(6409..6726)
                     /locus_tag="VW528_001096"
     CDS             complement(6409..6726)
                     /locus_tag="VW528_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9911924.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRVDRDQYIDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(6723..7022)
                     /locus_tag="VW528_001097"
     CDS             complement(6723..7022)
                     /locus_tag="VW528_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9911925.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(7272..7460)
                     /locus_tag="VW528_001098"
     CDS             complement(7272..7460)
                     /locus_tag="VW528_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEU9911926.1"
                     /translation="MKASTWVYDKGEWYYVSSSGAMLANDWVKDNGKWYYLASSGKML
                     RNTYTPDGYYVGNSGAWQ"
     gene            complement(7711..8385)
                     /locus_tag="VW528_001099"
     CDS             complement(7711..8385)
                     /locus_tag="VW528_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911927.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            complement(8391..8837)
                     /locus_tag="VW528_001100"
     CDS             complement(8391..8837)
                     /locus_tag="VW528_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9911928.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(8951..9454)
                     /locus_tag="VW528_001101"
     CDS             complement(8951..9454)
                     /locus_tag="VW528_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9911929.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(9451..9813)
                     /locus_tag="VW528_001102"
     CDS             complement(9451..9813)
                     /locus_tag="VW528_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEU9911930.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFLQAPYYVPERIAITGKICGVHIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(9879..11738)
                     /locus_tag="VW528_001103"
                     /pseudo
     CDS             complement(9879..11738)
                     /locus_tag="VW528_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(11777..12226)
                     /locus_tag="VW528_001104"
     CDS             complement(11777..12226)
                     /locus_tag="VW528_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9911931.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIDQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(12350..13066)
                     /locus_tag="VW528_001105"
     CDS             complement(12350..13066)
                     /locus_tag="VW528_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9911932.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(13947..15362)
                     /gene="ply"
                     /locus_tag="VW528_001106"
     CDS             complement(13947..15362)
                     /gene="ply"
                     /locus_tag="VW528_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9911933.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(15373..15705)
                     /locus_tag="VW528_001107"
     CDS             complement(15373..15705)
                     /locus_tag="VW528_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911934.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(15776..16384)
                     /locus_tag="VW528_001108"
     CDS             complement(15776..16384)
                     /locus_tag="VW528_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911935.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(16397..16855)
                     /locus_tag="VW528_001109"
     CDS             complement(16397..16855)
                     /locus_tag="VW528_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9911936.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            16980..17379
                     /locus_tag="VW528_001110"
                     /pseudo
     CDS             16980..17379
                     /locus_tag="VW528_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(18144..18404)
                     /locus_tag="VW528_001111"
     CDS             complement(18144..18404)
                     /locus_tag="VW528_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911937.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFFDG"
     gene            complement(18463..19014)
                     /locus_tag="VW528_001112"
     CDS             complement(18463..19014)
                     /locus_tag="VW528_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911938.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(19004..19201)
                     /locus_tag="VW528_001113"
     CDS             complement(19004..19201)
                     /locus_tag="VW528_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911939.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(19205..19999)
                     /locus_tag="VW528_001114"
     CDS             complement(19205..19999)
                     /locus_tag="VW528_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911940.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(20407..20832)
                     /locus_tag="VW528_001115"
     CDS             complement(20407..20832)
                     /locus_tag="VW528_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911941.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDNNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(20961..21344)
                     /locus_tag="VW528_001116"
     CDS             complement(20961..21344)
                     /locus_tag="VW528_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911942.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQLASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKKEMVLG"
     gene            complement(21366..21572)
                     /locus_tag="VW528_001117"
     CDS             complement(21366..21572)
                     /locus_tag="VW528_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911943.1"
                     /translation="MDLSNKASNLRKKLGADGEAPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(21559..21732)
                     /locus_tag="VW528_001118"
     CDS             complement(21559..21732)
                     /locus_tag="VW528_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9911944.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLYLSLNQSMIAK
                     MEKGERNITNGFK"
     gene            complement(22091..23047)
                     /gene="lytA"
                     /locus_tag="VW528_001119"
     CDS             complement(22091..23047)
                     /gene="lytA"
                     /locus_tag="VW528_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9911945.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADRPEFTVEPDGLITVK"
     gene            complement(23423..24793)
                     /locus_tag="VW528_001120"
     CDS             complement(23423..24793)
                     /locus_tag="VW528_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9911946.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(25107..26273)
                     /gene="recA"
                     /locus_tag="VW528_001121"
     CDS             complement(25107..26273)
                     /gene="recA"
                     /locus_tag="VW528_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9911947.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(26328..27584)
                     /locus_tag="VW528_001122"
     CDS             complement(26328..27584)
                     /locus_tag="VW528_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9911948.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(27669..28685)
                     /locus_tag="VW528_001123"
     CDS             complement(27669..28685)
                     /locus_tag="VW528_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9911949.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(28693..29211)
                     /locus_tag="VW528_001124"
     CDS             complement(28693..29211)
                     /locus_tag="VW528_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9911950.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(29201..29644)
                     /gene="tsaE"
                     /locus_tag="VW528_001125"
     CDS             complement(29201..29644)
                     /gene="tsaE"
                     /locus_tag="VW528_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9911951.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(29730..30350)
                     /locus_tag="VW528_001126"
     CDS             complement(29730..30350)
                     /locus_tag="VW528_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911952.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(30733..31596)
                     /locus_tag="VW528_001127"
     CDS             complement(30733..31596)
                     /locus_tag="VW528_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9911953.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
BASE COUNT         9535 a         6970 c         5363 g         9797 t
ORIGIN      
        1 aaaattctct atcgaaacat tgatatttga taataattca aaaaatgttg aaagagaaat
       61 ttcattttct cccctttcaa atctagaaag ttgagatttt gatataaaat cagctgcagc
      121 atactctaaa gaatatccgt ttaattttct aaaatatcga aaagcttctc cgtaattttt
      181 cacactttca ttcccttaac tttcttttcg agcgataaac ataccatatt ctttaggaat
      241 gatcaacata ccattattaa actcactttc caatctttta cgtattattt cctcttctag
      301 tgaacctatt tctgaaattc ctttaaacga ttggatgtat ttaactaggt catctatact
      361 agttacctgt aactcatcat catagagtaa tgtatcgaca gagttaaaat acctactcag
      421 gtacctttta ccattttgta aagtaaacgt tctattttct aaatcttgat taacctcatc
      481 tttaaataaa cttgccaaat aatttacaac tccattttca ccaaatgtag cacagtaaaa
      541 aatgcctccg gtttttaaga ccctattcac ctcagagaga gctttaggaa tatcattcac
      601 atgatgtaga agcatattag caataacaat atcaaaagtt tcattctcaa aagaaatctt
      661 ttgaatatcc attatctcat aattgacatt gtttctattt ccaatcactg acttcgttga
      721 ttttaccata tctttagaaa aatctgtaac aatcaactgc ttcattttat ctatagaatc
      781 actattactt ttccacaatt ctcctgtacc acatcccaat tctagaacct ttacttcatc
      841 agtgatttgg tagttagaaa atatccaatt attaaatccc aatttatttt ttgaatactt
      901 cttatgcaat tctactcgaa tatcaagatt atcatgactt ttatattgtt caattacttt
      961 atcaatcatt ttaaatgacc tccactctat aatttggcat tatatcacaa gaataaagat
     1021 agtgttaaat aagttatata aatgcaaaaa aggaatattc cgttatagca gagttgtaac
     1081 acatttctat aaagaaattt attcctacga ctcagtttgg catgcttaac ttcctaactc
     1141 aaaaacaaag catcgattaa tttatccgtt cttcttttga aacagatttg aactgataat
     1201 tgctcgtttt aatttaaaaa gctcttttga gcaaaacttc atttttttaa gagtccaaca
     1261 atttctgtct atcgcttttc tactagcaaa aaacctacct tgaggcaagt tttcatttat
     1321 atatctaatt tcgtaacagt atagtaaaat gaaataagga cagtcaaatc gatttctaac
     1381 aatgttttag aagtaaaggt gtactattct agtttcaatc tactataaat ttgatatgag
     1441 aatagtcttt ctcaacatcc ttatccaaca agcatgctgt ggcgtccttc ttaacttaaa
     1501 ccaggtcaat attctgggct tgggctgcat agacgcagcc acaataacat tgacgataga
     1561 tatcatactc ctcacacatc tctactgaac gtttgtagcc ttgatttttc ttgaaatcgt
     1621 tgggaagata gtggggcgtg taaatttttt gcacatcgat tccgatgcta ttgatagttt
     1681 gagaattctt atgaggactg atggtcaagg ctgaaccaaa gtagtcaaag cccaagtcca
     1741 tagccacttg cgctgtttta tccagtcggt agtcaaaaca aaccttgcaa cggtcgccac
     1801 cttcgggctc ctcctctagc cccctaacta gttttcggta ttaattgggt tcgtagggag
     1861 cttctaggta ctgaaccgta tttcctgtcc gctcattaaa atcactaaca aatttcttgg
     1921 tgacatagac ccgcttatgg tattctgcct tgggatggat attagaattg gcaaaataga
     1981 tggtcacatc tgcatacttg gtcaaatatt ctagtgtata ggtactacaa ggggcacagc
     2041 aaacatgcac gagaatggtt ggccgctact catttttttc ccatgcttgt accattttct
     2101 gcatgacacg gtcataatta atcttctgat tgggggtcat cttgcacaga atttcttcta
     2161 catcgatcat gttcttctcc tttttctagt cttattttat catataagtt aggagagctc
     2221 aaatcaattt agaagtgaat attataaaaa gaagggttag tcattccctc cttttgtgtt
     2281 ttttataagt tgttttctat agaaagctta catcatcccg cccatcatac ttggatccat
     2341 tgctggagct ggggctactg gttctggttt attggctacg actgcttctg ttgtcaaaat
     2401 caagctggct acagatgctg cattttgtag ggctgaacga ctcactttaa ctggatcaat
     2461 gataccttga tcaatcatgt taacccactc gccagttgct gcgttgaatc ctataccaag
     2521 ctcagcattt ttcaaacgat cgataacgat agatccttca aatcctgcat tgtgagcaat
     2581 ttgacgaaca ggttcttcca aagcacggag aacaatatta cgtcctgttg cttcatctcc
     2641 tgtcaattcc aaggtagcaa cagctggaat cacattggca agagctgttc caccacctgc
     2701 aacaatacct tcttcaacag ctgcacgagt agcgttgagg gcatcttcaa tgcggagttt
     2761 catttctttc aactcagttt cagttgcggc tccgacctta ataaccgcta caccacctga
     2821 caatttggcc aagcgttctt gcaatttttc acggtcaaat tcagaagttg tagtttcgat
     2881 ttgagacttg ataaccgcaa cacggtgaga aatcgcttca ggatttcctg caccttctac
     2941 aataaccgtg ctatctttgt ccacggtcac tctcgctgct tgaccaagag cttcaattgt
     3001 cgcatctttc aactcaagac caaggtcttc tgtgataact gttccgcctg ttaagatggc
     3061 gatatcttca agcatggctt tgcgacggtc accaaaacca ggtgccttga ctgctactac
     3121 gttgaaggtt ccacgaatct tgttcaaaac aagagttgga agagcctcgc catccacatc
     3181 atccgcaata atcaagagtg gacgattgct ttggagaatg ctttccaaaa gtggcaagat
     3241 ttcttggata ttggaaattt tcttgtctgt aatcaaaatg tacggatttt caaggtcagc
     3301 caccattttt tcgctatctg tcaccatgta ctgtgaaagg taaccacggt caaactgcat
     3361 tccttctacg acttcaagct ctgtttccat accacgtgac tcttcgatgg tgatgacacc
     3421 gtctttgcca actttttcca ttgcttcaga gatgtactca ccaacttttt cagaacgaga
     3481 agatacggct gcaacttgag cgatagcttc tttattggca acagggatgg cgttgttttt
     3541 caaagcttca actgctgcgg caactgctgt ttcaatccca cgacgaatac cgattggatt
     3601 tgcacctgct gtgacgtttt tgattccttc acggacgatt gcttgggtca aaacagttgc
     3661 agttgtagtt ccatcacctg cgatatcatt ggtttttgaa gctacttctg ataccaattt
     3721 ggcacccata ttttcaaaat ggtcttctaa ttcaatttct ttggcaatag tcacaccgtc
     3781 attggtaatc aatggtgagc caaatgattt ttcaagaacg acattgcgac cttttggtcc
     3841 caaagttact ttaacagtat ctgcaaggat atcgacacca cggaccatgg ctgaacgggc
     3901 atctgatgaa aatttaattt cttttgacat acttactttc tccttctatt cttcaatgat
     3961 tgccaagatg ttagcttcgc ctacgatgat gtacttttca tcgccatctt tgacatcaag
     4021 acctgcgtgg gcttcaacta agacacggtc tccagtttta acacttggag caaccaagtc
     4081 accgttcaag gtacgaacac cttgtccagt agccacaact tgggctgttt tggttttttc
     4141 ttgggctgaa cctgcgagaa caaagcctcc aacagtttgt tctttttctt ctacttttaa
     4201 gaccacacgg tcccctaatg gtttcaacat ctgatttcct ccataatgag atagatatta
     4261 ttagcactct ttatacctga gtgctaattc atagttctat tgtatcactt agtcagaaat
     4321 aatcaagaaa aaagtctgac tttctcaaga taaaaagcct gagaccaact cagacttttt
     4381 aatgtttata ctcaatgaaa atcaaagatc aaactaggaa gctagccgca ggctgctcaa
     4441 agcattgctt tgatgttgtg gataaagcta acgtggtttg aagagatttt cgaagagtat
     4501 taaaatggca attcttcctc ttccaagact aaatctgcca aatcttggcc tgcattattt
     4561 tcacgcatgg cacgttgagc acgactttcc aagagttgga atcctgttac aagtacttcg
     4621 gtcacgtagt tcatttggcc atttttctca aagcgacggg tacgcaattc tccatcaacg
     4681 gaaatgagac tacctttggt tgcgtagctt gccaaagttt ctgctagtct gccccatagg
     4741 accatattga caaaatcagc ttcacgttca ccgttttggt ctttgtaacg acggttgaca
     4801 gcgatagttg ctcgcgctac cgacttgtca ttgttggttt tgtgcaattc tggtgtagac
     4861 gttaaacgcc caatcatgat aactttatta tacatatttt cttcctccta cttatctatt
     4921 cgtaggaaat caaaaaaagt tacagaaatt tgtaactttt cgaggaaatt tcttattttt
     4981 tatgaatcat gaaacctgtc gcctgttgat tggccataat ggtcatatct gtaatctgaa
     5041 cacggcgagg ctgactggtc acatagacta ctgtgtctgc aatatcctga gcttgcaagg
     5101 cttctattcc ttggtaaacg gacgcagctc gctctttatc accatgaaaa cgaacagttg
     5161 agaaatctgt ttcgacaatc ccaggctgaa tggttgtcac cttgatatcc gttgcgatgg
     5221 tatcaattcg cagtccatcc gaaaaggtct taaccgcagc cttggtagct gagtaaacag
     5281 cggcaccagc ataggcgtag attcctgcgg ttgaccccat attgataata tgaccttgat
     5341 tgacttttat cattgctggc aagaaacagc gagtaaccgc catcagacct ttaacattgg
     5401 tatccaacat ggtcaacata tccaactcct catagtcttg atagggagcc aagtcaagag
     5461 ccagtccggc gttgttgacc aagatatcaa tcctccctat cgtttctaga atatcagagc
     5521 aaacagtctt caccatgacc atatccgtca catctagtgg aaaagtccag acggtttgat
     5581 ttggaaagct tactgcaaac tccgacttga ggatttctaa tctgtctatc cgtcgtcctg
     5641 ttagaacgac atcctcaccc tgctccagat aagcacgcgc aatcgcttca ccgattcctg
     5701 aggttgctcc tgtaatcact acattttttg ccatcttatt tccttctatc agatgctgac
     5761 aagttcctag gcagtccagt gtttcgctgg gtcgaacggt gttccgacaa cttggtcttc
     5821 tgataattca agcaccccac gtttttgtgg agcatttggc agatgcaatt cacgaggact
     5881 gcacatcata ccaaaactct tttcaccacg aagttcacct gggaaaatga gattcccttt
     5941 tggcatcata gctccaggaa gcgcgacaat ggttttcaac cccacacgcg cattgggagc
     6001 tcctgcaacg atttgtacag tcttatcact tgcgactgca acttggcaga tgttgaggtg
     6061 gtcactatct ggatgggcta ccatctcaac aatttcaccg acaacaaact taggttcctt
     6121 atcattaaca atttcttctg taaaaccttc cgcctgcaac tcttggttca aacgagcgac
     6181 ttgctcatct gtcaaaaaga cttgaccgcg ctctgcaatt tcaaataaac ttgaaacttc
     6241 gaaaatattc caagccactg tttccccatt atctttgaga aaaacacggg ctaccttgcc
     6301 tttgcgctcc acatccagtt tggcatctcc gctatttttc acgatgacca taaggacatc
     6361 accgacatgt tctttattat atgtaaaaat cattgtttcc tttttctcct atttcagtcc
     6421 tgctaaaaag tcattgattt gttgcttgct tttacggtcg cgattgacaa aacgaccgat
     6481 ttccttgtcc ttttctagaa caacaaggct agggattcca tatacatccc agagtttggc
     6541 caaatctata tactggtctc tatccactcg aataaaggta aactctggat tagtctcctc
     6601 aatttctggt aaggcaggat agatataacg gcaatcgcca caccagtccg ccacaaaaag
     6661 gaagaccttc ttaccgtctt tttctactaa agatgccaat tcttctaaac ttgctggttg
     6721 tatcataaga cttcctcctc atagactagg tcttcatttt catagacaaa agtataatga
     6781 cggccatcct caaaaatgac gccaccaagc aagctctcta gactgctttc atagacttga
     6841 acatagaggg tcgcaatttc ccccatgtct gaaaaatggt ctcgtacaat cgctgtcaac
     6901 tcttcctgag tcttcatgag cttatggtca tctgcaactt ttttcgtagc aagggcaagg
     6961 ctcccaatac caagcagagc cagacctgcc atccacattt ttttagcttt cataccattc
     7021 attttaacac aaaaaaggct ttaggacaaa tgaggaaaca gcaaaaaagc aagtaaaaag
     7081 cctcttcctt taaggaaaag gacttcttat actcaatgaa aatcaaagac caaactagga
     7141 agctagccgc aggctgctca aagcactgct ttgaggttgt agatagaact gacgaagtca
     7201 gctcaaaaca ctgttttgag gttgtggatg aagctgacgt ggtttgaaga gattttcgaa
     7261 gagtattatt cttattgcca ggcgcctgag ttaccaacgt agtaaccatc aggtgtgtag
     7321 gtattgcgaa gcatcttacc tgatgaagct agataatacc acttgccatt atctttgacc
     7381 caatcattcg ctagcatggc tccagaagaa cttacataat accattctcc cttgtcataa
     7441 acccaagtgc ttgctttcat ggctcctgag caattaaagg ttcaaaaaac tgtccaatat
     7501 cattcatttt tttaaagcat tttacactac atcatccacg ctgtaggaca tagatacatc
     7561 aaacgtaaga gccaaaatat cgaaaaatat acaactttta aaggcaacat ttcatttttt
     7621 aaagtctaac aaccatagaa ctgaaatcag agcaaggcat caacttccta ctcttagtac
     7681 gtctattttg atggttgaag tgaagatttt ttatttaacg tagacaagct ccaacttccc
     7741 aaaagccaca tctccacgga taatcaaggt tttgctggtt ggggctacag gagcgtcagc
     7801 cttagctgca ccaaaggagg tttctacttt caaatctaca cgccagtgtt gtggaacata
     7861 gacggttgca ttcccgaagg ccacttcaat atttaaagtt gcaaaatcac ctagcatctc
     7921 tgcattatca tagtagattt tagcatcccc aaaagcgact tccacttggt catctacgag
     7981 atcttgatct tgcttataga aggtcccgct accaaaagcg acttccttat ccgtgacgac
     8041 tgtttttttc ccattgtacc accatttttt ttcattccag aacttacttg aatgcgtcag
     8101 ataaccaaca ccaagtacca ccaagatgct agcccaaata agagaatgat tggtaactgg
     8161 taacaagtca taagcgtaat ttgcaatgat gagcgccagt aaacctgtaa aaactgccga
     8221 agtgagatga cgtctaagga tggactcaat ggacttataa gcaaaaaaaa caatacctag
     8281 taaaggccat attttaccat ccaaagaagg aattccaaaa ttcccttgca gcaagatcca
     8341 agctgctaaa accaataaaa caataccaaa tgctttcttt ttcatgtttt ttacctcatg
     8401 tttcttagat tttcttttag gagggattgg taatgccgtg agacatgaac ctccttgtgc
     8461 gtctgataaa aggaaatggt gcccgttcct gaaaaggact tgtccactga gtaaatctga
     8521 cggatgtttg cgatcgttga cttggaaact cgattaaaat agcgaggcaa gatagactcc
     8581 aactcataga gcttgaggcg aacctcataa gcttccttct ggttatgagc gtagatcttg
     8641 ctcccttctg tttcaaagaa gagaatttct gacaggtcta gataatattc acctgtcccc
     8701 ttgtaaaaag tcaacctcgg agcctttgac tcttgcaaga gccgttgcaa gtctgcaatt
     8761 tcatctgtca aagtgggggt cttgatgaca atttcagtct cctctaaatt gccgtcaatc
     8821 tcaattcgta acttcatcac atctcctttc tgactctact atatcaaagc taagttagga
     8881 atacaagggc aaaaaagcaa gtggtcaatt tgaagcagta agtggttgta ggactagctt
     8941 aacttacatg tcagaatagg aagaaaagga atccccacac cagaccacaa gcatagccaa
     9001 ccaagacatc cttgggataa tgaacacccc ctaaaaccct caccaacccc agcaaagctg
     9061 aaaagatcaa gcatactaag ccaataggta gactagcatg cagacaggcc atggagatga
     9121 tagttgcaga aaagacatgg cgactgggca tcgaccgtcc cgaactatcc ttatcaagaa
     9181 ggggacagat atcccaagtt tcataaggtc tcgggtgatt gattttctta cgaaacaggg
     9241 acaaggccac aaaacctgat gcaggaataa gcacataaac cccaacttgc tgaccaagtc
     9301 ctagttgcag gtaagtggtg actagcaaga tcagatagac caggggcatg gcgactgtca
     9361 tcaaacgatt gaaactgcgt aataggaata aaagaattgg acgactagtt agtctggagc
     9421 tgatatttcg ataccattct tgataatttt tcatttatgc ttctcatact tttctagaac
     9481 ttgttgagat gttttcccct tggcaagcca atccactagc ttgtccaaac gacgtatttc
     9541 ctgcatcaga ggatcatcaa tttcctcaat atggacaccg caaatcttcc ctgtaattgc
     9601 aattcgttca gggacataat atggtgcttg tagaaaaaaa tctccgtaag taacatccct
     9661 atccagacaa gcaagaacgt cactcacttc ataccctgtc aaccaactgg tcacttggtg
     9721 aactgactct gcctttccac cctttcgctc aacttttgca atcaaggcta agtagacagc
     9781 agcaaatttc atattgtata atttctgact catacttctc tctttctatg tgtactagta
     9841 taaataagaa aaagaaggcc gtcaagcctt tttttgattt attcttctgc ttcatcttct
     9901 gtaaattgac tattgtacaa gtcagcgtag aagccacctt gcgccatcag ttcctcatag
     9961 ttgccttgct cgatgatatt tccatctttc atgaccaaga tcaagtctgc atttcggatg
    10021 attgacaagc ggtgggcaat gacaaaggat gtgcgtcctt ccatcaaacg gtccatggct
    10081 ttttggatca attcctctgt ccgtgtgtca acagaagaag tcgcctcatc caaaatcaaa
    10141 agcgatgcat ccttaagaag ggcacaagca atagtcaata gttgtttttg tcctacagac
    10201 aaggtcacgg tgtcatccaa gatggtatca tagccatctg gcaaggtcat aataaagtgg
    10261 tgaattccca cagccttact agcttccatc attcgttcat cactaatccc tatttgatta
    10321 tagatgagat tgtctcgaat agttccttca aagagccagg tatcctgcaa gaccattgaa
    10381 aaggcatcat gcacttctga acgcgtcata gccttggtat ccacaccatc aatgcgaata
    10441 cttctcttat caatctcata gaatttcatc aaaagattga caatggttgt cttaccagcc
    10501 ccagtcggcc caacaatggc aaccctttga ccagcatgag ctgtcacaga gaagtcatag
    10561 tcttgaacat tgacaccgtc caccagaatt tctcctgctg acacgtcgta gaaacgtgga
    10621 atcagattga ccagagttga tttaccagaa cctgttgacc caataaaggc cactgtttga
    10681 ccagtttctg ctttaaagct aacatgttca ataactgcct ccgaatttgc cgcatagcgg
    10741 aaggtcacat ccttaaactc gacctgacct ttgaagtttt catcagtcag ctgcacttga
    10801 acagggtttt ggatagaaga atgcaaatct aaaacttgat taatccgctt agcagagacc
    10861 atagttcggg gaagaacgat gaagagtgct cccatgagaa ggaagcccat gacaacctac
    10921 atggcataag acatgaaaat aatcatgtca ctaaagagag gcagacgcgc tatcggagca
    10981 gcgtcgttaa tcacataggc cccaatccag taaatcgcta cactcaaacc acttgaaatc
    11041 cccatcatga taggattcaa aatagccata agacggttga caaacaaatt caaacgggtc
    11101 aattcatcat ttactgctgc aaatttttca ttttgataat cctctgcatt gtaggcacga
    11161 acgacacgaa tacctgttaa actctcacga gtgatactgt tcagtttatc tgtcaacccc
    11221 tgaatcaagg actgttttgg aaaggctagc gtcatcaaaa cggtcgtcat caggacgttg
    11281 acaatcacta ccacaagtac ggcccagagc cagtattctg aatgacctaa aatcttccca
    11341 atagcccaga tagccataat tggaccacgc gttaccactt gcaagcccat agtaatcaac
    11401 atttgaactt gagtaatgtc attggtagta cgcgtcaaga ggctaggaat tgaaaatttc
    11461 ttaatctctg tctgcgagta atccaaaact cggttaaaaa tatcacttct cagcctacta
    11521 gtataagaag ccgccactcg ggatgcaaaa aatccaactg caactacgga caagaaggca
    11581 agaaaggaca ttcccatcat catgcttgcc ggctgccaca actcatctaa attagtttct
    11641 tgactaccta gcaaatccgt aattttcgag atataggtcg gcacttccaa ctctagatag
    11701 accgaaaagc aagtaaagag aatggctagt aaaatcatcc cccattcttt tctactaatt
    11761 cttttggcta atttctttat tctctcctcc tattcccttg atattttgcc tgtagttgac
    11821 cgagaacctt ctcaaaaatc agtaattcat cttcatcaat atcttccatc aactgcttgt
    11881 ctatgcgttc aaaaaaagcc ttaacctgtt gcatctgaga acgtgctttg tccgtcagac
    11941 gaacaaactt agcccgctta tcaacaggac tcgcctccaa ttccaccaaa ccattttgca
    12001 ctatacgctt aaccagatta ctagcaacag acttggtaat attgagttcc tgatcgatat
    12061 ctttaatcaa gaccaagtct tggtttttct cgcgattatc caaaaaatgc acaacctgac
    12121 cttgcggccc acccataaat tcaatgccgc aacgtttggc ttccttttgc accatcaggt
    12181 gaatttgatg accaaaacgc ttaaagacta acatcggttt acccataatc tcccccttct
    12241 aaataaaaat agttctctgg agaataatta aatttctatg agaactattt tcttgattaa
    12301 aaaaatccca agtgattttc tcacttagga tcatgttcta taggttaaat taaaacccat
    12361 ctacgttcgt ataaatcttt tggacgtctt cgtcgtcttc aagaacgctg taaagttttt
    12421 caaaggtttc aaggtcttcg cctgacaatt ccacttctga ctgaggaatc atttccaatt
    12481 cagtcacttg gaattcttca ataccagact cacggagggc aacgatagcc ttgtgaaggt
    12541 cagttggcgc tgtgtaaact gtgattgtac cttcttgtgc ttctacgtca tccacatcca
    12601 catccgcttc gagcaattgc tcaaagactg cgtccgcatc ttcacctcca aatacaataa
    12661 cacctttgtt gtcaaagagg taagaaacag aacctgaagc gcccatgttt ccgccgtttt
    12721 taccaaaggc tgcacggaca ttggctgctg tacggttaac gttagaagtc aaagtatcca
    12781 caattagcat agagccattt ggcccaaaac cttcgtaacg tccttctgta aaggtttcgt
    12841 ctgtgtttcc tttggcttta tcaatcgctt tatcgataat gtgttttggc acttgggctt
    12901 gtttagcacg gtcgataacg aatttcaaag ctgagtttga ttctggatct ggatcacctt
    12961 ttttagctgc tacatagatt tctacaccaa attttgcata tactttagag ttggctccat
    13021 ctttagccgt tttcttggct acgatattgg cccatttacg tcccattagg aatctccttt
    13081 tttcacattt taatctttct tattataaca caagtttttt tgattttcac tagaggaaat
    13141 ggattttatt agcaaatcaa gctaggatag cactttacct gctaagatgg tcttgccttt
    13201 ctatctttat caacagacac tcatccacat tcaaaaaaca aactagacca ttatctgcaa
    13261 atagaaagtt tcagccaagt ttgacaaagt cagctcaaat tactgtttga agtttgtaga
    13321 tataagcgac aaaaacaatc atactgcacc ttttgttgac agtctactcc agacatatca
    13381 tagttcaagt aaatactttg aaattcaaca gttcttatag gcgctattgt attctaagaa
    13441 atcaatagaa gagtttctaa gcaaacctct aatactcaat aaaaatcaaa gagcaaacta
    13501 gaaagctagc cgcaggttgc tcaaaacact gttttgaggt tgcggatagg gctgacatgg
    13561 tttgaagaga ttttctaaga gtataattta cgtgttccta agatggagaa gttagactag
    13621 tacactggca cttctaaacc attgctagca attgatttgt tcatatttaa tttcattttt
    13681 tccataaatg ggtattagat ataaacagca aaatatttcc gatacgtgtc attcttgaat
    13741 ttccaatcat ctaaaacaag taaaggataa tcaatcccct gtatatcaag gaattggcta
    13801 ccctttttac ttttttacac atcctgtttg atagactcat tttaacatca cgagcatact
    13861 ccaatggaaa tcgctaggca agagataaac tttcagatat ccgcagagag atcatcgcct
    13921 ctttttgtcg caagcattct cctctcctag tcattttcta ccttatcctc tacctgagga
    13981 tagagagttg ttccccaaat agaaatcgtc cgcttacgca ctagtggcaa atcggttttt
    14041 tcataaaccg tacgccacca ttcccaggca agcccggtac actctctaat tttgacagag
    14101 agattacgaa cattcccttt taaaggaata ctagtggtaa agtgagccgt caaatcctgc
    14161 ccatttctgt cccaagcctt aggagtcaag acttccttac cttgatgatc ataggataat
    14221 tcatcccaag taatataata ttgggcaaca taggcaccac tatgatccag cagtaaatct
    14281 ccgtttctgt aagctgtaac cttagtctca acatagtctg tactgttttg aaaggtcgca
    14341 actacattgt cacgtaaaaa agaagttgta taggaaatcg gcaagcctgg atgatctgct
    14401 gtaaagcgac tgccttcttg aatcaagtcc tctaccatat ccaccttgcc tgttacaact
    14461 cgggcacccg aacttgggtc gccccctaaa ataaccgcct tcacttctgt attgtccaaa
    14521 atctgcttcc actctgtctg aggagctacc ttgactcctt ttatcaaagc ttcaaaagca
    14581 gcctctactt catcactctt actcgtggtt tccaacttga gatagacttg gcgcccataa
    14641 gcaacactcg aaatatagac caaaggacgc tctgcagaaa ttcctctctg ttttaaatcc
    14701 tctaccgtta cagtatcttg aaacacatct cctggatttt taacagcgtc tacgctgact
    14761 gtataataaa tctgcttaaa attaacaatc tgaatctgct tttcacctga atggacagag
    14821 ttaaaatcaa tatcaagaga attccctgtc ttttcaaagt cagaaccaaa cttgaccttg
    14881 agttgttcca tgctgtgagc cgttattttt tcatactgca ttctagctgg gacattattg
    14941 acctgaccat aatcttgatg ccacttagcc aacaaatcgt ttaccgctcc gcgaacactt
    15001 gaattgctgg ggtcttccac ttggagaaag ctatcgctac ttgccaaacc aggcaaatca
    15061 atactataag tcatcggagc acgatcaacc gcaagaagag tgggattatt ctctaacaag
    15121 gtctcatcca ctacgagaag tgctccagga tagaggcgac tgtcgttggt agctgttaca
    15181 gaaatatcac ttgtatttgt cgacaagctc cgcttctttc tttcgataac aacaaactca
    15241 tcgggtagct gattaccctc tttgatgaaa cgattttcaa tactttctcc ctgatgggtc
    15301 aagagtttct ttttatcgta attcatagct agtataaagt catttactgc tttatttgcc
    15361 atcttctacc tcctaataag ttcctggatt gagttgcata aactcagact tgttcagcga
    15421 aatcagccgt ggttggacta agtgatccaa aatttcctcg tacaattctt ctgagacatt
    15481 gcgtcgccgt ctggctagat aagaagtcgg aataaccgta ttatccaaca taaatacctt
    15541 atctaagtca atcaaggttg gtcttgtaaa aggattacga gctagatccg gctcttctat
    15601 cataaagttc ttgaccaaac gtctggtcaa gagagctggt ttgaaggtct gatttttaac
    15661 caactctttg tttttagtca tgctgttgtc aatacagata tacatatgat tcttcacagc
    15721 caaatcgcta ctaatagtcg gaaaaggcaa ataaagagct acaacatctc ctctcttaat
    15781 caagcaagag cacccccttt tctcctaatg taacatagac aggattgacc aagtcttctg
    15841 attgactcag aatttccaaa gtttgagttt ggcgcgctgt caatttagta gcatcttgtc
    15901 tcttcaatac aaaatgcttg tcgccaataa ccttgacaat ataatccttc tccaaagctg
    15961 actggtaaat ccacatcaga tgttgtctgt cctgagaact caagagagaa ggattttcaa
    16021 gcctcccgat agtctgataa aaatcaaaaa caggagctaa ctcctgccaa tctgattggc
    16081 tagttgtcaa ggctagaaaa agggctttgc gagctgatac ttcttggtta gccttgagag
    16141 ttactttccc ctccaagttt tttagaaatc gggaaactcc agaaagcaaa tttttctcta
    16201 actgcgagaa ataaaaacct ttcgttccca gacataagtc tttcatgtcg ctttctctag
    16261 caaataagag ctcaaacatt tgatagtaaa agaaaaatat ctggcactgg gtcgcgctca
    16321 tcttttcctt atcggcttct ttttttaacc agagcaaggg cgacaggtaa ctggattgag
    16381 acatttcctc tacctcctac tcttttttaa ctggagcatc tgcactagct gccacttctt
    16441 ttgactggat actttcccac tggttaatct cctctgagat aagaccttcg catgtcttga
    16501 caaatagggc aaaagccttg gtctttcctg catatttctc cgtttggcat tgatagagga
    16561 atttttcttt ctccaggagt tgcgcagttt tttggtaaga aatccaattt tcctttgcat
    16621 tatacaaatt gataatcccc tcacacagca agccgagact ggataaggca accgaaatca
    16681 aacggtagcg atcacctggc ataggaatag cacaaaagac agctatgagg aaacctgcca
    16741 cgatttctgt tatttttaat accttatagc gcctacgatg ttgaacgctt ttctttaaaa
    16801 aatgagctat ctgtacgtct aatcgctctg tcaggtacat ttcttctggc gtcatattcg
    16861 taactccttt catttacttt gataaccata gtataacata tttgaatatc aaagtatagt
    16921 aaaaactgga ctataactta ttagacttcc tgcgaaacaa aatatggtat agtagttcta
    16981 tgaatgatga agcaagtaaa caactaactg atgcacgatt taagcgtctt gttggtgttc
    17041 agcgtaccac ttttgaagag atgttagctg tattaaaaac agcttatcaa aaaagtcgga
    17101 caagctggta aaatcttggc tgacagtaat tatcaagggc tcatgaagat atatcctcaa
    17161 gcacaaactc cacgtaaatc cagcaaactc aagccactaa cagttgaaga taaagtctat
    17221 aaccatgcgc tatctaagga gagaagcaag gttgagaaca tctttgccaa agtaaaaacg
    17281 tttaaaatgt tttcaacaac ctgtcgaaat catcgtaaac gcttcggatt acgaatgaat
    17341 ttgagtgctg gtattatcaa tcatgaacta ggattctagt tttgcaggaa gtctattata
    17401 tatatgcaca gacatatgaa atctttttga tacagccttc tttttacata gagagacagc
    17461 agttatgata tatttatcca ttcacaacat cttctgtacc aatactacaa taattggtac
    17521 attttgatta cacaatcgtt tcaaaaaata tgatggaaga ctgagtattt tatagtaatt
    17581 cccttcctat caactagatc aaccagttga gtgttgaatg ttgaaacacc ctagcctcag
    17641 caaaggacta tgatcagcat ttcctagcca ataggacgct ccaaatcacc tattctaagt
    17701 ccatcactag cttggtcagt aagatacaga ctgcaaggca agactgacgt ggttgatgag
    17761 attttggaag agtattacaa tcatagattc accagtagac ttgaccgcag atttaactat
    17821 agtagattga aactagaata gtacacttct gcttctaaaa cattgttaga aatcgatttg
    17881 actgtcctga tcaatttgtc ctgttctcgt ttcattttac tatactaaaa aaccacctga
    17941 aacaaatcaa gtgactagag gataaaaaca aagactgggc aaaaagtttt gaaaagcaaa
    18001 aaacgcataa tatcaggtct tgaaaaacct tgatactata cgtttgattg tgggaagatt
    18061 gacttcattt tctcctgaaa ttgaagtttt ttctagtttc caggtataga cgaattcaat
    18121 atagccatct cgctacaatt attttaacca tcgaaaaaat ttttcagaat ttcaaaaatt
    18181 tcattttgat ccaatgaaat gtcattattt tgaagaatag ttgacttcac aggctctaat
    18241 tctatcacaa agcggtgcat gtctgctttt accgcactac ttgatgttat agccgataac
    18301 ttttcatctc ctagcaagga agctgtcaaa cgggtaaggt cattcaaatg ctttttaatg
    18361 gacttactta aaccttgagc acctgtggca gagcgctccc tcatatccag ccaagcttta
    18421 gccttaaaaa taattaatac tcaatgaaaa tcaaagagca aactaggaag ctagttgcag
    18481 gctgctcaaa acactgtttt gaggttgtag atggaagctg acgtggtttg aagagatttt
    18541 cgaagagtat agaccacaat tactcaatac cgaatacccc tgaatggttt cttttacatg
    18601 caccaatata ttataataat cttcatccaa taataaggct gataaactag catcttggtc
    18661 aaaatataag ggaatttctc gaccgtcctt ctttaatgga tattctggta agatactaaa
    18721 tagttcaatc atagaaggaa actcagaatt agttgttgta aatcgaaaaa gctgcgcttt
    18781 ctcatctttc tgacttcctt gatactctcc caattctaaa aaatgattca aggtagtata
    18841 aaattcctta ttttttactt catcaatgat gaccatatca taatcttttg ttgtgcgact
    18901 tttaaatcct tgcgaatcca atacgataga ggtagcagtt cccccaatca gaacataata
    18961 gttctgaaaa tccgcaaaca tttcttgaaa aataactttc gtattagctg gcatcatctt
    19021 ctcccagata ctgtaatatc atattttcta gtgcttcact ctcttcctct atacgtgggt
    19081 catcatcatc ttttaaggtc aaataaagag aaatcggatc tacaaattgt ttatcatgat
    19141 tatttttaaa atcattccaa aactcagata caaaaggacg atatttccat atctctagca
    19201 tctttcattt taaaacatgc tgagaaagtg gcaaggataa ctgattgaat tttctctgcc
    19261 atatgacata gctaatattt tcatccgttt cagctaaaaa agttgaatgc gacaaagcat
    19321 aagcaccacc atatagaagg ttagaaacag attttatctg ctttatatcg ccatctggca
    19381 ataaaatccg ttttttgatg ggattaaata aacatgacac ggattttaag aataattctt
    19441 tctttgacac cgtatatgtg taaagcttat tttgcttgtt taaccaatat aaagctttaa
    19501 aagtcctcaa acacctataa attgttgagt ttggaagtcc agtgacttgt gaaagcaaat
    19561 caacatctac tactttttga ccttttgtca ataaaaaggc aatccacgtt aattgttcgc
    19621 taggtgttaa ttccttaggg acttcagtat cattcgcatt gagtactagt cccaatggag
    19681 ggaagaagag gtttccctta aagtctacaa acggaactct agcttgaagt aattgctttt
    19741 tttcactgtc tgataacttc gaaaacacca aaacaacatc catattggct ttttcaccca
    19801 tagtgcgagc ctgagtaaca aatgaactca aactccccct tctcttttct ttaatcaata
    19861 aaaatgattc cttatgaaaa gtcatttgag tatactcaaa cctttttaca aaactgattg
    19921 gtaaagtcaa agtcagttcc tcaatactga catcaacata aaaggattga aaaattgaca
    19981 ttatcttttt gacattcatg ttttttcctt attctatcat ttttattata ttttatcatt
    20041 cggtataata aaatataagt tttttgataa aataaccaaa aactactcgc tatcccctct
    20101 cacattaccc tacatatcgt ttgacatgcg actgatagtt caggaaaact tcaaggagaa
    20161 cttttctctc atccactatg caggacttac tatttcactt ctactcctat aggctcaatt
    20221 tgacactttt ctttttcgaa ttgctcatat gcctcctgaa tagtgagttc gatttatcca
    20281 aatttatttt tgtctatttt gatgaatact tccacgaaga tagtctgaaa atgaatttgc
    20341 accaattttc cttctctttt taagcatatt attccagata tgattgccct cttgttcagt
    20401 aataaattac gctttaaacg cttcaatcag tatatctcct gttgtcatat gttctaaaga
    20461 aaattcttct acatatgatt taacatctct taggttatta cttcctaata tcccattatg
    20521 ctttttcgct aaggaaatag aagccgcttc tcccttacca ataatcttgt tattatcatg
    20581 atttcttgtt aaatctctat ataatgcgta ttcttcagtt ccaatgtcta tgctcacaat
    20641 ctcagctgaa cccttagcta ccaactgatc tatcctagat tttaaatggg gaattgtagg
    20701 tatattgatt tcatcataca cctcttgtgg aataacaatt ttacccgaat agagcttttc
    20761 taaaagatgt tcagtgccaa cccataaaaa tactgaaata caatctgcat caataaatac
    20821 acgactagtc aaacgacaac tcccccctct tcatctagcc catatacaat atcatatctg
    20881 aaagcatcta gtaacagttc ctcatacttc ccttgcgaaa ttctgttatt ttctaaaagt
    20941 tgttcagttg aattaatata ttatcctaat accatttcct ttttactttc tgacaaagga
    21001 cgatataaac ttgtatcata gcctaatctt gaagctgtct ctataacact aatatccata
    21061 tttttaattt cttctgcatc aaggtatcca tcattcctca atctatataa catagcttta
    21121 tgactgatac cataaaactg acccaatttt ataatatctt ctacttcaag atgagttcta
    21181 ttggcatttt ctctgatttc ctcaaccatc ctatacagtg aagatgggaa aattaaaaaa
    21241 taagaagcaa gctgatccgc ttttctttca gtttcatctc cttcaccaat caagataaga
    21301 ctgactgaac tcttcttcac ctcatcataa taaagatgat acagttcatg tgctaaagaa
    21361 aatctttacc ttcctaatga catgtctgaa ttgactgcaa tgagactgaa ctgagttcct
    21421 ttataacaga ctccgctaat attctttccg agtccataaa ataccagcgt caaattttct
    21481 atcttttgta ccaatttaaa aatatctatc ggcgcttcac catcagctcc caactttttt
    21541 ctaagatttg aagctttatt acttaaatcc attcgtgata ttcctttcac ctttttccat
    21601 tttggcaatc atgctttgat tcaaagacaa atataaagac aaatattcag caatcttatc
    21661 ttgagtaaga tttgaagata ttctaagttc ttttattctt tttccaacat cacatacatt
    21721 tatcacatac atttatcata ttcatacacc tctcataaaa agaatagcac acttttgtca
    21781 taatttttta aataaaaaaa ttatgacaaa acaaggaagc aatttattga tgctgcttaa
    21841 aaatctaaaa ttgatgagtt cctatcaaaa atcgtatctt caacctcaaa acagtactta
    21901 aagctatccg actcggttta cattgtcaaa tttagatttt atttgagcat aactttctag
    21961 tttgctttct gatttttgtt taatatagta gcaaacattg aaggaaatat ctccacaaga
    22021 aaacgcatac tattaagctt tttcaagacc taataatatg cgctgttctg atttgaaaga
    22081 cattccatta ttattttact gtaatcaagc catctggctc tactgtgaat tctggcctgt
    22141 ctgccagtgt tccgtctggt ttgaggtagt accagcctgt tccgtccgct gactggataa
    22201 aggcatttga taccatggcg ccttctttag cgtctaagta gtaccaagtg tccttgtact
    22261 tgacccagcc tgtcttcatg gcaccttctt cgttgaaata gtaccactta tcagcgattt
    22321 tcttccagcc tgtagccatt tcgcctgagt tgtcgaacca gtaccagttg ccgtctgtgt
    22381 gcttcctcca gcggtctgca agcatatagc ctgaactgtc aaagtagtac caagtgccat
    22441 tgattttctc aaacttgtct tttggataag agccgtctga atgtacgtac cagtagccag
    22501 tgtcattctt ctgccagcct gtttcaatcg tcaagccgtt ctcaatatca tgcttaaact
    22561 gctcacggct aatgccccat ttagcaagat atggataagg gtcaacgtgg tctgagtggt
    22621 tgtttggttg gttattcgtg caatactcgt gcgttttaat tccagctaaa ctccctgtat
    22681 caagcgtttt cggcaaacct gcttcatctg ctagattgcg taagagttcg atataaaggc
    22741 ggtagtccgt catgaactct tctttggttg aatggctttc aatcagttca accgctgcat
    22801 aggtctcagc attccaaccg cccccaacgt cccaggcacc attatcaaca ggtcctacct
    22861 gcatgatgca accgttccca acaatgtgcg agaaaaaacc taattctggg tctttccgcc
    22921 agtgataatc cgcttcattc tgtacggttg aatgcggatt cccagttgag tgtgcgtgta
    22981 cttgcctata tggttgcacg ccgacttgag gcaaatctgt tcttaattta ctcacattaa
    23041 tttccatatt ctactcctta tcaattaaaa caactcattt tttacaatcc agaaccagaa
    23101 actcctttat ttctacctta caaagaagac aatcttagtc acgattaggc ttgtagatag
    23161 aacttcaaaa cgcactattt tgacactgta aataggactg acaaggtctg cattctatct
    23221 acaataacac cccagactaa aaagcttttc aaaagtatat ttttacagtc tctatatgtc
    23281 cttttcataa atactatact ttattatatc atataaaaga agtcaaaagt ctgttaaact
    23341 attttcaaca ccaaactaaa gacgagaaca caagtttttt cgattttcac tagaggaaat
    23401 ggattttatt cagtaaatcc aactaggatt gcactttggt tgccaagatt gccttgcctt
    23461 cttttatcaa gggatgacgg aataatgaga agtacagttg aactgtcatg gcaacccaga
    23521 taagaggttc acaaaggata acacccttat atcctgccca aggaataatc aaaaccacaa
    23581 aaacgatttt tccgattagt tcaataaagc tagaaactag aggaaggatc ttttgcccca
    23641 agccctgcaa gcaattgcga taaatcaaca agaggctcaa aatgggataa aaggttgaac
    23701 tgatttgcag atagagactt ccattttcta tcaagtaacc atctgtcgaa ctagccaaga
    23761 aggaaaccaa agctggactg gcaaaaaaga ggaaaataca aacaaaaact gcccaggata
    23821 tacttaaacg actgccgatt cgaagacctt gaacaatacg gtcaggtcgc ttagctccta
    23881 gattctgaga agcaaaggtc gtcattgatg cagaaatagc ggtcatagga agaagggcaa
    23941 aggtcataat gcgtcgagct gccgtctggg cactaataat cactgcacca aatgtattaa
    24001 cagaaaactg taaaatcaca ctgccgatag atacaattga actcatcaag cccatagcca
    24061 aaccttgctc caagagatcc gcgtacaagc ttttgtccca tttgaaatgt ttaaactgtg
    24121 gcaagagttc tggcacactt ttacgaatat aataaaagca gagaaccgct gataaacctt
    24181 gcgaaataat ggtagcaagt cctgcggatt gaactcccag atgcaattgc gtaataaaat
    24241 agagatccag aaccacatta accaaggcag agaaaatcag aaatcccagg gctgctagac
    24301 tgtcaccaat agaccgcaac aagcctgcaa aaagattata agcaaagctg acacctacac
    24361 aggtcacaat catagaaata tattgataag attgaggaag aatttctgca ggagtatcta
    24421 agtattgcaa gagaggatac aagccaagaa agcccagcaa cataactaga atgctcaaaa
    24481 gagcacctaa aatccaggtg gctgctactg cttccttgat tttagtgaaa ttccgagccc
    24541 cataataacg agcaatgaca atccccatgc cattgccaac accaagtgta aagcctacaa
    24601 tcaggtcaaa aatcgctgtc gtcgctccta ctgcagccaa ggattcttga ccaagaaatc
    24661 gtccaacaat caagacatca gcagtgttat agagctgttg aaaaatattt gatagcaaga
    24721 ttggaaaggt gaagcttaag agcgagggaa gaatcggacc atgtatcagg tccactgttc
    24781 gttttttatt cataaagcta ttatatcagc tttccagagg agcgacaaga aacagcaaac
    24841 ttatatatag cggaatgaaa taaggacagt caaatcgatt tttaacaatg ttttagaagt
    24901 agatgtgtac tattctagtt tcgatctatt atagttggtt gagtttggaa tagtacgctg
    24961 tagctgctaa aacatttcta gaaattaatt tgactttcct aataaagttg ttcatatctt
    25021 atttcaagct actatatata atatataatc taaaaacttg aatcaaaaat cgaaaaagta
    25081 gcggatttct ccactgcttt aacagcttat tcttcaattt cgatttcaag ttcatcgcct
    25141 aagtcaagcg gaacttcttc attcactgct tcttctttct ttggctcatc ttttttgttt
    25201 tcagtatctt gttctgaaac ttcttctcca tcaatcaagc caaatttaga acggacttgc
    25261 ttatcaattt catcaaagat ttctgggtgc tctgccaagt atttcttagc attctcagaa
    25321 ccttgcccaa ttttttcatc tttgtaagaa taccaagccc ctgctttttt gataatatcc
    25381 aaatcgcttg caatcttcaa aagctcacca gtcttagaaa ttccttctcc gtacataatt
    25441 tcaactacgg cttccttaaa cggtggagct accttatttt ttacaacctt aatcttagtt
    25501 tctttaccga cattggtttc tttttgatca ccagttccct taatttgtgt attaccacga
    25561 acatccaagc ggactgaagc atagaatttc aaagcacgtc cgcccggtgt tgtttctgga
    25621 tttccaaaca tcactccaac tttttcacgc aattggttga taaaaatggc aattgttttg
    25681 gttttattga tagaggcgcc aagtttacgc atggcctggc tcatcatacg agcctgcaaa
    25741 ccaacatggc tatctccgat atctccatca atttccgcac gaggaacaag ggcagcaact
    25801 gagtcgacta cgacaagatc aactgcacct gagtcaatca attttcccgc aatctcaaga
    25861 ccttgctctc ctgagtctgg ttgagacaag agcaattcgt caatattgac accaagggcc
    25921 gcagcataag ctggatcaag ggcatgttcc gcatcgataa aggcagcaat cccaccttct
    25981 ttttgcgctt gtgcaactgc atgaagggca accgttgtct taccagatga ctctgggcca
    26041 tagatttcga tgatacgtcc cttaggataa ccacctgagc caagggcaat gtcaagagct
    26101 aaagaacctg agctcatcac ttgcaccttt tgctccgcac gttcacccaa acgcatgatt
    26161 gatcctttac caaagtcttt ctcaatcaat ttaagagcgt cattcaaggc cttttcacgt
    26221 tctgccccaa atttttttga aatttcttct aatttttttg gttttttcgc cattctattc
    26281 tcctacattc taatgatcct cagacctatc tactattata tcaaaagtta gtcacttaat
    26341 aaagccttgc gaactaggtt aaaggcatgc ataaccgcaa tgtgacgtac atctgctcgg
    26401 cttctgcctc caatattaac cttgataacc tcagttcctt gatcttgcgc caagcctatg
    26461 aagactgtcc caactgggtg tccttctagg ctatctggtc ctgccactcc agtcaaacta
    26521 attccaaaat cagactgggt cttgcttcgt gcctgctcag ccatcttctg agctgtaaat
    26581 tcagacacca caccatgttc ttccaaattc ttggcaggaa tatccaacat ccttgatttt
    26641 tcctccaagc tataggtcac aaaaccaccc tcaaatatac ttgaaactcc agaaaaattc
    26701 gccacggtag cttggaaaag acctgccgtc aaactctctg cagccgcgat ggttttccct
    26761 tgccttttca gttcttctac cacaatgctg gctaaactag tttcttcccc ataaccatag
    26821 caaaagtctc gtaaagaaat tccttcgaaa gtctggcagt ccaagatttg attttccaag
    26881 atatccagcg cttgattcgc ctcttcttga ctgctagcct ttgttgacag acgtagagtg
    26941 acttctcctg tcttggcata aggggccaag gtaggatcga tctgattatc aattaaatca
    27001 gccaaaatcg taaccaactg gctctcgcca atcccaaaga aacgaagaac tcgggaatac
    27061 agcttgctcc ctgtcatcaa cttgggtaga agttggttta agaccatggg tttcaattca
    27121 cttggcggac ctggaaggac gacataggtc actccgtcta cttctaattt tcctccaaca
    27181 gccagtcctg tttcgtttgg cagtggaatc gctccttcta caatttgagc ttgtctttcg
    27241 ttattcggtg ttcgggcata gtctggtcgc agggcaaaaa agatatccaa cttctcctga
    27301 gcctgaggat caaagactaa tgctttccct aaaaatttag ctagggtttg tttggttagg
    27361 tcgtcctcag ttggccccaa accgcctgtc aaaatcacca gactgctacg ttgactggca
    27421 atctcaagca aagacaagag acgaacttca ttgtctccta cagccgtctg aaaatataca
    27481 tctaccccaa tctcagctag tttttccgac aaaaactggg cattggtgtt gacaatctgt
    27541 cctgtcaaaa tctctgttcc aacagcaatg atttctgctt tcatgtttcc tcctacctat
    27601 ctattcgtat ttttttgaaa aaatcgcagg aattttccta cgattgattt ttttatttgt
    27661 atcaaaagtt aattatcttc atcaccaaca ggtgctctgc caaataaatc ttcaaataaa
    27721 accgcattgg tttcaagctg agtaacttct tcttgtccca aagaacgtcg gagtatattt
    27781 tgcatttcca acatatgtgc tctcgaaaca atctggtaag aaacaccttg aagtatctct
    27841 ccttcaccct gcaactgctg agtttcaatg gttttaaatg aatctttata gcctagcaag
    27901 ttagggatac tttttgcaga caaatcaata ttggtctgca tattgtcact caaagctttt
    27961 agaatctctt gataatgacc aatgctattt aaactgagag ctttttccat gactttttga
    28021 ataacttcac gttgacgttt ttgacgacca taatccccct caggatcttg gtaacgcatt
    28081 cgtgcataga ctagggcttc ttctccccca atatgttgct ccccaacacc gatagaaatg
    28141 gtattaaatt cttcttggtc actgatagaa attgggaaac ctaggatatt attgactgta
    28201 atacctccta ctgcatccac taatttttgc aaccctctca tattgaccat cacatagcga
    28261 tcaatatgga tattcatcat tttttgaatg gtttctatag caagctctgc tccaccatct
    28321 gcatatgctg agttcagttt cgcttcatga gcctgaccat tccctgattc aatgcgcgtc
    28381 agaatatccc gctctaaact catcattgtt gtttttttcg ttttaggatt cactgtcatc
    28441 aagatcatgc tatcacttct accgacccaa gtttcagttc gttcaacatt tccggtgtcc
    28501 actcccatta acagaatggt tagaggttca gtcgcttcaa taaccttggt ttcttcaccg
    28561 atttttttat aggttttagc taaggtttct gtcccttgtt gataaatagt ataagcaaaa
    28621 acacctactc ctactacagt tacagaaagt aaagctagca ccattccaat aattttttta
    28681 accatatttc tactaaccta tcagtttacc catcaagtaa acatcgataa atttcccttc
    28741 ttctatatat gccccacgct cttggctacc ttcaatgaca aagccatgct tttgataaag
    28801 atggactgct gcttgattac gagtttggac agtcagttgg agacgacgca gaatgccact
    28861 tgcttgtgcc cactctatcg cttcttctag caacaaactt cccaagccat tattccaata
    28921 tctttttcca atcacaatga agagatctcc aatatgacgg actctcttac gctgatcagc
    28981 tgtaatattt acaataccag caattttgcc atttaagaat gcaagtaagg ttatctgatt
    29041 gtccgaacta gcttgcttgt tgaggaatat ttccatctcc tcactagtca agagaatacc
    29101 atctccgtct aggctggtaa agtctgtctc caaactcaca cgatttaaaa aggccactaa
    29161 ttcagctgca tctttgggct ctgcttccct aatgagcaat tcatactcca tattgaagct
    29221 cctctaacaa tttctcagca cgcaaaccct ttgcctgaaa atttaaacgg cgtccatctg
    29281 cttcttttag aatttccaat tctaaataag catctggcaa ggcatctcct aagagatttc
    29341 cccactcaat aacagtcacg ccgccaccaa agataaactc atccaagtcg atagaatcag
    29401 catctccttc aatacgataa acatctaggt gataaagtgg aagtcgacct tcatactctc
    29461 tcacgatagt ataggtggga cttttaatca tttgagaaat ctgtaatcct tttgcaagtc
    29521 ctttagtaaa ggtcgtttta cctgcaccca gttctccagt taagattaaa acatcattct
    29581 ttgctaatag atggcccaaa cgctccccta aggcttgcaa ctcttcttca ttttttgtgt
    29641 acatactctt attataccaa aaacttttct tttgtgtcta ttttcctact aaacttatca
    29701 tcataacatc cataaaaaac aggctttctc taaaagaaaa tgagcgtaac aatgaccaat
    29761 acaagatctc ggaaaatatg accataaaag gaaacttcct tcttaaccga atttgggaca
    29821 agataggctg caaaaaacaa gcccagtcca atataaatca gaagtgagac aatggtcatt
    29881 ggatttctta agaaaagaag tgttgctaaa atagtcacca acactgtctt ttttctgtcc
    29941 agcatagcaa gaaaatcgcg cacgtatttt ttcaagggta aaaaaatcag caaatctagc
    30001 ccaaatagga aaaagaagga tggcaataaa aagtcaacta attcttgctg cagcgtattt
    30061 ttgatgaaca agttatctga caaaacaaga acagctccta acaaattaat taagagtaac
    30121 atactgtaaa aaagcttcac cgacttctta ctggctagga cactatggac ttcttgctta
    30181 cgggtataaa gataatttac tccagcacag attcctgaaa cgaaaaccat gcttccgatg
    30241 aaaaaagctg tactttgttt aaaggacaag atgcattcct tccataggaa acagctactc
    30301 aaactgattt gaattaaagc taacaaaaat aagattctca ttgatttcat cttctctctc
    30361 ccttcctacc aatcattata ctaggagaaa agagagaact gtttctaatc ttctcaaatg
    30421 tctctttaag acgctaaaca aacactagag actaatactc aatgaaaatc aaagatcaaa
    30481 ctaggtagct agccacaggt tgctcaaaac agtgttttga gattgcagat agagctgacg
    30541 tgatttgaag agattttcga agaatataaa tttgaaatca tgaaaatccg tcaaacgggt
    30601 gattgttttg tctcgcacct cacggagcga gacggactca gagtcacata attataaggc
    30661 tgatagtatt aatctaacta tcagccttac aggttattta acgtttcaga aaaactataa
    30721 tgtcaagatt aactaaacag tatctagttc cttcaaataa ttttctatct tcatcaacat
    30781 taaaggattg ttataaatct tacataactc tcttgcttct atataataat ttttgacttg
    30841 ttctctgtct agaaatttgg ctccagcatt tcctacaaga ataagtagag gagccaattg
    30901 gtagcttgtc tgtctttgtt tacagagttc aatcgtttca agagcttctt ggatggcttc
    30961 attatatttt tcctttgata ctaggtagtg agcgtagttg taacgaactc tgatgtagcc
    31021 aaataaaaac tcttgatggt ccaaattttt tgtctgatac aactctatta aatgagagta
    31081 gtttgcctca tattcttgtt cacgacccac taaggaatag aaattagata gagtattcaa
    31141 cgcctttaaa taaatcagag tatttgaaga gacttttaat aatatatttt ccaatgacga
    31201 aattgcctca cacttactgt catattgata gaagtcaatt atagatttaa tccattcaag
    31261 gtaagttcgg tcttctaatg ttagaaaagt gcttcgttct acctctattt tataaagata
    31321 ttctaaatcg tcataatttc tgtcatctaa taggcgagca gatagatgtt tgaaattaga
    31381 gaggttagac ttaacttcga tttgttcatt gaaaaagtaa tccaaaggga cttcaagtcg
    31441 ttgagagagt ttgaataaca agtctgcgga gggaataaaa tgacctcttt caattttact
    31501 aatctggctt tgttcacaaa ttccttctgc aagagtttgt tgggagagtc tcaactcttt
    31561 tcttttcaat ctgaatttct ctgcgagtgt attcattaaa gataataaac cttcctattt
    31621 gcttaatttc attataaagt ttttagttca aaatagcaag ttaaa
//
LOCUS       DAVKQB010000025        30685 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKQB010000025 DAVKQB010000000
VERSION     DAVKQB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30685
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.025"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            26..389
                     /locus_tag="VW528_001128"
                     /pseudo
     CDS             26..389
                     /locus_tag="VW528_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828316.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            365..679
                     /locus_tag="VW528_001129"
     CDS             365..679
                     /locus_tag="VW528_001129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911954.1"
                     /translation="MDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAM
                     VTKLLCIYSEGDSFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVPKLILSWSH
                     RN"
     gene            739..1956
                     /locus_tag="VW528_001130"
     CDS             739..1956
                     /locus_tag="VW528_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9911955.1"
                     /translation="MDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTD
                     GNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQLIGG
                     QDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYE
                     LYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEIS
                     KIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDRSVDTFD
                     FAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSHYHIGPS
                     YFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQKDQAVA
                     DDNEGDENDDADY"
     gene            1943..3259
                     /locus_tag="VW528_001131"
     CDS             1943..3259
                     /locus_tag="VW528_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9911956.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSNVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            3434..4420
                     /locus_tag="VW528_001132"
     CDS             3434..4420
                     /locus_tag="VW528_001132"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9911957.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4484..4555
                     /locus_tag="VW528_001133"
     tRNA            4484..4555
                     /locus_tag="VW528_001133"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4517..4519,aa:Arg,seq:ccg)
     gene            4738..5436
                     /locus_tag="VW528_001134"
     CDS             4738..5436
                     /locus_tag="VW528_001134"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9911958.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFNQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVIIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5427..8966
                     /gene="smc"
                     /locus_tag="VW528_001135"
     CDS             5427..8966
                     /gene="smc"
                     /locus_tag="VW528_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9911959.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKAGQQKAELETAKEQVQK
                     LLADYQVCAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLEIILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVHYQTALEIALGASSQHIIVEDEESATKAIDLLKR
                     NRSGRATFLPLTTIKARTISSQNQDTIAASPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNLDAIEQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEVIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8963..9757
                     /locus_tag="VW528_001136"
     CDS             8963..9757
                     /locus_tag="VW528_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9911960.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            9757..10575
                     /locus_tag="VW528_001137"
     CDS             9757..10575
                     /locus_tag="VW528_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9911961.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLQAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10579..11868
                     /gene="ftsY"
                     /locus_tag="VW528_001138"
     CDS             10579..11868
                     /gene="ftsY"
                     /locus_tag="VW528_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9911962.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTVEENNSEVLEPEAPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDVLRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVTEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11914..13401)
                     /locus_tag="VW528_001139"
     CDS             complement(11914..13401)
                     /locus_tag="VW528_001139"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9911963.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(13550..14290)
                     /locus_tag="VW528_001140"
     CDS             complement(13550..14290)
                     /locus_tag="VW528_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9911964.1"
                     /translation="MVKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14290..16455)
                     /locus_tag="VW528_001141"
     CDS             complement(14290..16455)
                     /locus_tag="VW528_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9911965.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            16625..17578
                     /locus_tag="VW528_001142"
     CDS             16625..17578
                     /locus_tag="VW528_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9911966.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            17655..19643
                     /gene="uvrB"
                     /locus_tag="VW528_001143"
     CDS             17655..19643
                     /gene="uvrB"
                     /locus_tag="VW528_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9911967.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            19653..20216
                     /locus_tag="VW528_001144"
     CDS             19653..20216
                     /locus_tag="VW528_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9911968.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            20182..20670
                     /locus_tag="VW528_001145"
     CDS             20182..20670
                     /locus_tag="VW528_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9911969.1"
                     /translation="MTVIIKSMETPKEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            20744..21211
                     /locus_tag="VW528_001146"
     CDS             20744..21211
                     /locus_tag="VW528_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9911970.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(21208..21723)
                     /locus_tag="VW528_001147"
     CDS             complement(21208..21723)
                     /locus_tag="VW528_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9911971.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(21735..22262)
                     /locus_tag="VW528_001148"
     CDS             complement(21735..22262)
                     /locus_tag="VW528_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9911972.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(22544..23107)
                     /locus_tag="VW528_001149"
     CDS             complement(22544..23107)
                     /locus_tag="VW528_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9911973.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(23091..23642)
                     /gene="coaC"
                     /locus_tag="VW528_001150"
     CDS             complement(23091..23642)
                     /gene="coaC"
                     /locus_tag="VW528_001150"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9911974.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(23654..24343)
                     /gene="coaB"
                     /locus_tag="VW528_001151"
     CDS             complement(23654..24343)
                     /gene="coaB"
                     /locus_tag="VW528_001151"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9911975.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            24573..26243
                     /locus_tag="VW528_001152"
     CDS             24573..26243
                     /locus_tag="VW528_001152"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9911976.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKSQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            26571..27746
                     /gene="mutY"
                     /locus_tag="VW528_001153"
     CDS             26571..27746
                     /gene="mutY"
                     /locus_tag="VW528_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9911977.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            27801..28505
                     /locus_tag="VW528_001154"
     CDS             27801..28505
                     /locus_tag="VW528_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9911978.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            28498..29847
                     /gene="vicK"
                     /locus_tag="VW528_001155"
     CDS             28498..29847
                     /gene="vicK"
                     /locus_tag="VW528_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9911979.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            29849..30658
                     /locus_tag="VW528_001156"
     CDS             29849..30658
                     /locus_tag="VW528_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9911980.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
BASE COUNT         9413 a         5468 c         6854 g         8950 t
ORIGIN      
        1 atcattagta aaaggagtta gattgatgaa taaaagaaaa gttagtttag aagattttta
       61 taaatggtat agtctaaata aagaagagtt attaaataag gcaactgttg gtgaaaagtt
      121 taatgataaa ttaaaagaag agtttctcca ggaatggcct ttggatagga ttttaacaat
      181 gtcaatcgat gaatatgtaa taggaaaggg acagcaaaat aagtctttat gctacgctct
      241 tgagaaggga aaatacaaaa atctatttct tggaatttct ggtggctcag cttcaaaatt
      301 tggtatttat tggaataaaa aaacaaacaa atataaagat caagctaata atgagatttc
      361 agagttggat cagcgatttt caaaattaaa atcagatttg tatgaaatta tcaaagaagg
      421 tattcgtttt aactttgaaa atcctatttt tgatatgaaa agatcaacaa atgaatttat
      481 tggtcgttct gctatggtga caaaattact ttgtatctat tctgagggag attctttctt
      541 tggtgtaaat attaatagtc agaaagaatt ttggaaccac tttgtttctc agacaaatca
      601 aggtggacct tatctgcaaa atcataaaat aattgaactg gtgccaaaac ttatcctgag
      661 ttggagccat cgaaattagg aactatgctt tttgagtatt ctaagctttt tatggaaaat
      721 aaggaagaca atagtacaat ggattcatca aacaattttc gtcatcaatt aactcaatct
      781 ctattaaagt ctccaaacct tatccttcgt ggtgctcctg gcacaggaaa gacttatctt
      841 gctaaagaaa ttgccaaaga attaacggat ggcaacgaag accaaatcgg atttgtacaa
      901 tttcacccat catatgatta tacggatttt gtagaaggtt taagaccagt atcaaatggg
      961 gatggagcta ttgagtttag gctacaggac ggtattttta aagatttttg tcagaaagca
     1021 aaagaaaccc aattgattgg aggacaagat aattttgatg aggcttggga ttcttactta
     1081 gaatatataa atgttgctga agaaaaagaa tatataacaa aaacatctta cttatctgtt
     1141 aatagtagac aaaatttgtc agtaaattat gatagtggtg ttccaggatg gtcactacct
     1201 agcaaatatg tttacgagtt gtataaagat aaaaattata ataagcaaga atactacaaa
     1261 agtggtggaa aaactgtcct agaaacattg agaaagagat ttggtttgaa agactatgtt
     1321 tccccaacag aaattgatac tgataagaaa ttcgtcttca tcatcgatga aatcaatcgt
     1381 ggggagattt ctaagatttt tggcgaactc tttttctcta tcgaccctgg ctatcgtggt
     1441 gaaaaaggaa gtgtttctac ccaatatgca aatctacacg aaactgatga aaagttctat
     1501 atccccgaaa atgtttacat catcggaact atgaatgata ttgatcgttc agtggatacc
     1561 tttgattttg ctatgcgtcg tcgttttcgt tttgttgaag ttactgccga gggtcaagtt
     1621 ggcatgttgg ataaagagct gaatatccat gcagaagaag caaaaattcg tctaagaaac
     1681 ttgaacgctg ctatcgaaaa tgttcaggaa ttaaacagtc attatcatat tggaccaagt
     1741 tattttctta agttgaagga tgtagatttt gactatgaat tactctggtc cgattatatt
     1801 aagcctctcc tagaagacta cttgcgaggt tcttatgatg aggttgaaac tttggaaact
     1861 ttgaaaaaag catttgaact gacaaataat gagcaaaaag atcaggcagt agctgatgac
     1921 aatgaaggtg atgaaaacga tgatgcggat tactgataat caacacaaga ttattaaaga
     1981 aaaatttgtt gaagaatatc ctaaactaag caatcttctt ttagacagaa ccttggaaag
     2041 tctatcccaa gatgaacgta ttttcatttt tccaaatgat ttgactcata ctcctgattt
     2101 ggataaggac caaaagattt ttgaaacagt caatcagaaa atcaagacag ggaacgtgat
     2161 tggttttctt ggatatggtc aggaaagatt aacgatttcc tcacgatttt ctgatgagag
     2221 taatgaccat tttttgcatt atctcttaaa caaggttctt catatcaatc tcactagttt
     2281 agatgttgct ttgtctcgtg aagagaggct ttatcaactt ttggtgtatc tctttcccaa
     2341 gtatctacaa gctgctattc gaaaaggtct ttataaggaa tatcatcgat tttctcataa
     2401 cgacagtaat gttaagggag tgattgatgt aagaagccat ctcaagaaaa atcttccttt
     2461 cacgggaaat attgcctacg caacgagaga gttcacctat gataatcccc tcatgcagtt
     2521 ggtccgtcac actattgaat acattaagaa tcagaaaagc attggtcaag gggtactaga
     2581 taatctctca actagtcgtg aaaacgtatc tgaaatcgtg cgtgtaacgc cctcttataa
     2641 actagctgat cgtgctaaga ttattcgggg aaatcaatct aaacctatac gtcatgcata
     2701 ctttcacgag tacagaaact tacaagaact ttgtctgatg atcctaaacc aagaaaagca
     2761 cggtttaggg tatcaagatc aaaaaatcta tggtattctc tttgatgttg cctggctttg
     2821 ggaagagtat gtttacacct tgttgccaaa aggttttgta catcccagaa ataaggataa
     2881 gacggatgga atttcagtat tttctgttgg gaaacgaaaa gtatatccag atttttatga
     2941 cagagaacga aagattgttc tagatgcaaa atataaaaaa ctggaattga ctgaaaaagg
     3001 aatcaaccgt gaggacttat tccagctgat ttcctattct tatattttaa aagctgagaa
     3061 ggctggactg atttttccta gtatggagca gtcagtaaat agtgaaatag gaaaagtagc
     3121 tggctatgga gctcaattga agaagtggtc tattcgaatc cctcagaatg cctcattcta
     3181 tagtacattt tgtaaaatga tggaaaattc agaagaaaat tttaaagcga ttattgatga
     3241 agaagtgggg agaaaataat ttcccctctt tcttttattt ttaacacaag taaaacccga
     3301 acgatatatt ttaattcata aaataatttg acaaaaactg tactttggtt tataataaat
     3361 taaggaaacg tcggaaaagg cgtagtgatg caagagcata ggtaggtcat tacaaaagaa
     3421 acgagacatc gatatgttaa atgaatttcc aatttttgat tacgaagata ttcaattgat
     3481 tccaaataaa tgtgtcatta aaagccgtgc agaagcggat acaagtgtca ctttaggaaa
     3541 tcacaccttt aaactacctg ttgtgccagc gaatatgcag acgattttgg atgaaaatgt
     3601 agcagagcaa ctggctaaag gtggttactt ctacattatg caccgttttg atgaggcagg
     3661 acgcattcct tttattaaac gaatgcacga tcaagggctc attgcttcta tctctgtcgg
     3721 tgttaaggat tatgagtatg atttcgttag acagctcaag gctgatgctc ctgagtatat
     3781 cactattgat attgctcatg gtcatgcgga tagcgtgatt tctatgattc aacacatcaa
     3841 gaaagaattg ccagatacct ttgtcattgc tggaaatgtg ggaacaccag aagctgtgcg
     3901 tgaattggaa aatgctggtg cggatgctac taaggtcgga atcggtcctg gtaaggtttg
     3961 tatcaccaag gttaagactg gttttggtac aggtggttgg cagttggctg ctctacgctg
     4021 gtgtgccaag gctgcgcgta aaccgattat cgctgatgga ggaattcgta ctcacggtga
     4081 tattgctaag tctatccgct tcggtgctag catgatcatg attggttccc tctttgcagg
     4141 acacattgaa agtccaggga aaacgattga agtcgatggt gaacagttca aagaatatta
     4201 tggttcagcc tcacaatatc aaaaaggtgc ttacaaaaat gtggaaggca aacgtatctt
     4261 gcttcctgct aaaggtcatc tgcaagacac tttaactgag atggaacaag accttcaaag
     4321 tgctatttct tatgcaggtg gacgtcaggt tgctgacctt aaacacgttg attatgtgat
     4381 cgtgaaaaac tccatctgga atggggatgc ttcccactaa gacggggtct ttgaccaaaa
     4441 aacttgttat tagagcaaat ttctgttata ataaaacaag tttccaccct tagtgtaatg
     4501 gatatcacgt aagattccgg ttcttgagat gggggttcga ttccctcagg gtggatgtaa
     4561 acatcctaaa aagcctttaa tagggctttt ttctttatcc tgacccaaat ttgccgctaa
     4621 aactgagaat ctctgtctaa agaattccca aatgctttcc agtcccgttt tagagtgact
     4681 cagggggctt tttttgatat aataaaaagg actattatca gttagaaaga ggttggtatg
     4741 aaagaattac aaactgtact aaagaatcat tttgcaatcg aatttgcaga caaaaattta
     4801 ctggaaactg cctttactca tacgagttat gccaatgagc accgcctctt aaaaatttca
     4861 cacaatgaac gcttggaatt tttaggagac gctgttctac agttattgat ttcagaatat
     4921 ctatataaaa aatatcctaa aaagcctgaa ggtgacctat caaaactccg tgctatgatt
     4981 gtccgtgagg agagtttagc tggttttgcg cgtgattgcc agtttaacca gtttatcaag
     5041 ttgggtaaag gggaagaaaa atcaggtggt cgcaatcgtg acaccattct tggtgatgcc
     5101 tttgaagcct ttcttggtgc ccttcttttg gataaggatg tggccaaggt caaggaattt
     5161 atctatcaag tcatcattcc taaggttgaa gcaggcgagt ttgagatgat tacagactat
     5221 aaaacccatc tccaagagtt gcttcaggtc aatggtgatg tggctattcg ttatcaggtg
     5281 atttctgaaa cagggcctgc tcatgataag gtctttgatg tagaagttct tgttgaaggt
     5341 aagagcatcg gtcaaggcca aggtcgttct aagaaattag cagagcagga agctgccaaa
     5401 aatgccgttg agaaggggct ggattcatgt atttaaagga aatcgaaatt caggggttca
     5461 agtcttttgc tgataagacc aaggtcgttt ttgaccaagg tgtgacggca gttgttggac
     5521 ccaatggatc tggaaagtcc aatattacag aaagtctgcg ttgggctttg ggggagtcta
     5581 gtgtcaagag tctccgtggg ggcaagatgc cggatgtcat ctttgctgga accgaaagtc
     5641 gcaaaccgct caattatgct tctgtagttg tgactctgga taatcatgac ggatttatca
     5701 aggatgcagg tcaagaaatc agggtggaac gccatatcta tcgtagtgga gatagcgaat
     5761 acaagattga cggcaagaaa gtccgtctgc gtgatattca tgacctcttc ttggatactg
     5821 gattgggacg agattccttc tctattattt cccaagggaa ggttgaggag atttttaatt
     5881 ccaagcctga ggaacgacga gctatttttg aagaagctgc tggagtttta aaatacaaga
     5941 ctcgcagaaa agaaaccgag agtaaactgc agcaaactca ggataatctg gaccgcttag
     6001 aggacattat ctacgagttg gataatcaaa tcaagcctct tgaaaagcaa gctgagaatg
     6061 cccgtaagtt tttagacttg gaaggacaac gtaaggctat ttatttagat gttctggttg
     6121 ctcaaatcaa ggaaaataag gctgaactag agtcgacaga agaagagttg gctcaggttc
     6181 aagaactctt gatgagttat taccaaaagc gtgaaaaatt agaagaagaa aatcaaactc
     6241 ttaaaaagca acgccaagat ttacaggctg aaatggccaa agaccaaggc agtttgatgg
     6301 atttgaccag tctgattagt gacttagaga gaaaattagc cctatcgaaa ctggaatctg
     6361 agcaagtagc cctcaatcaa caggaagcac aagcccgttt ggctactttg gaggataaga
     6421 gaagttcact cagccaagaa aaatctgata aagaaagctc tttagccctc ttggaagaaa
     6481 atctagtcca aaataatcaa aaactcaatc gattagaggc tgaattgcta gctttttcag
     6541 atgatcctga tcagatgatt gagctcttac gtgaacgctt tgtggccctt ttacaagaag
     6601 aagcagatgt ctcaaaccaa ctgacccgta ttgagaatga gttggaaaat agtcgtcagc
     6661 tttctcaaaa acaagcagat caactagaaa agctgaaaga acagttggct acagctaaag
     6721 agaaggctgg tcagcaaaag gctgagcttg aaactgccaa ggagcaggtt cagaaattat
     6781 tggccgacta tcaagtctgt gctaaggagc aagaggagca gaaaacttcc tatcaagctc
     6841 aacaaagtca actctttgac cgtctggata gtctcaaaaa caagcaggct agagcgcaaa
     6901 gtttggaaat tatcctgaga aatcatagta acttttatgc aggtgttaag agtgttctcc
     6961 aagagaaaga tcgtctaggt gggattattg gagcagttag tgagcacctg acctttgatg
     7021 tgcattatca aactgcccta gagattgcgc ttggagcgag tagccaacat atcatcgtgg
     7081 aagatgaaga gtcggcaacc aaggcgattg atttactcaa acgaaacaga tctggtcgtg
     7141 caaccttcct tcctttgacc actatcaagg ctcgtacgat ttctagtcag aaccaagata
     7201 ctattgctgc aagtccagga tttctgggca tggcagatga gctggttact ttcgatacta
     7261 gactggaagc cattttcaag aatttgctag ctacaacggc catttttgat accgtggagc
     7321 atgcgcgtgc agcagcgcgc caggttcgtt atcaggttcg tatggtgaca ttggacggaa
     7381 cagagctgcg cacaggtggt tcttatgcag gtggagctaa tcgccagaat aacagtattt
     7441 tcatcaagcc agaactggag caattacaaa aagaaattgc tgaagaagaa gcaagtttgc
     7501 gttcagatga agtgagtttg aagaacttgc aagatgagct agctagattg acagaaagat
     7561 tagaagccat caaatctcag ggagagcagg ctcgtattca ggagcaaggc ttgtccctcg
     7621 cctatcagca gaccagtcag caagttgaag agctagaaac tctttggaaa ctccaagaag
     7681 aggaattaaa tcgtctttct gagggagaat ggcaagcgga taaggaaaaa tgccaagagc
     7741 gccttactac aatcactagt gaaaagaata atctggaagc tgagattgaa gagattaagt
     7801 ctaataaaaa cgccatccaa gaacgttatc aaaacttgca ggaagagcta gctcaagctc
     7861 gtctacttaa gacagaactg caagggcaaa aacgttatga agttgctgat attgaacgct
     7921 taggtaagga actagataat cttgatattg agcaagaaga aattcagcgc cttcttcaag
     7981 aaaaggttga caatcttgag aaagttgata cggatttgct cagtcaacag gctgatgaag
     8041 ccaaaactca aaaaacaaat ctccaacaag gtttgattcg caagcagttt gagttggatg
     8101 atattgaggg tcaactggat gatattgcta gtcatttgga tcaggctcgc cagcagaatg
     8161 aagagtggat tcgcaagcaa actcgtgctg aagccaagaa agaaaaggtt agcgagcgct
     8221 tgcgccatct acaaagtcaa ttaacagacc agtaccagat cagctacaca gaagctttag
     8281 aaaaggcgca tgagttggaa aatctcaatc tggcagagca agaagttaag gatttagaga
     8341 aggctatacg ctcactgggt cctgtcaatt tagacgctat tgaacagtac gaagaagttc
     8401 acaaccgtct ggatttccta aatagccaac gtgatgatat tttgtctgcg aaaaacctgc
     8461 tccttgagac cattacagag atgaatgatg aggtcaagga acgctttaaa tcaacctttg
     8521 aggtcattcg tgagtccttt aaagtgacct tcaagcagat gtttggtgga ggtcaggctg
     8581 acctcatttt gactgaagga gacttgctga cagctggggt tgaaatttct gtccaaccac
     8641 caggcaagaa aatccagtct cttaatctca tgagtggtgg tgaaaaagcc ctatcggctc
     8701 ttgccttgct cttctccatt attcgtgtca agactattcc atttgttatc ttggatgagg
     8761 tggaagctgc gctggatgaa gccaatgtta aacgttttgg ggattacctc aaccgctttg
     8821 acaaggacag ccagttcatc gtcgtaaccc accgtaaggg aaccatggca gcggctgatt
     8881 ccatctatgg agtgaccatg caagaatcgg gtgtttcaaa gattgtttca gttaagttaa
     8941 aagatttaga aagtattgaa ggatgacaat taaactagta gcaacggata tggacggaac
     9001 cttcctagat gggaatggac gctttgatat ggatcgtctc aagtctctct tggtttccta
     9061 caaggaaaaa gggatttact ttgcggtagc ttcgggtcgg ggatttctgt ctctagaaaa
     9121 attatttgct ggtgttcgtg atgacattat tttcatcgcg gaaaatggca gtttggtaga
     9181 gtatcaaggt caggacttgt atgaagcgac tatgtctcgt gacttttatc tggcaacttt
     9241 tgaaaagctg aaaacttcac cttatgtaga tatcaataaa ctgctcttga cgggtaagaa
     9301 gggttcatat gttctagata cggttgatga gacctatttg aaagtgagtc agcactataa
     9361 tgaaaatatc caaaaagtag cgagtttgga agatatcaca gatgacattt tcaaatttac
     9421 aaccaacttc acagaagaaa cgctggaaga tggggaggct tgggtaaacg aaaacgttcc
     9481 tggtgttaag gccatgacaa ctggctttga atccattgat attgttctgg actatgtcga
     9541 taagggagtg gccattgttg aattagttaa aaaacttggt atcacaatgg atcaggtcat
     9601 ggcttttgga gacaatctta atgacttaca tatgatgcag gttgtgggac atcctgtagc
     9661 tcctgaaaat gcacgacctg aaattttaga attagcaaag actgtgattg gtcaccataa
     9721 ggaacggtcg gttatagctt atatggaggg cttataatgg cagatataaa attgattgca
     9781 ttggacttgg acgggacctt gctgactact gataaaaggc tgacggatcg taccaaggaa
     9841 accttgcaag ctgcgcgtga tcgtggtatc aaggtcgtat tgacaactgg tcgtccctta
     9901 aaagccatgg atttctttct ccatgagtta gggactgacg gtcaggaaga tgagtatacc
     9961 attactttta atggtggatt agttcagaaa aatacaggag aaatccttga taaaacagtc
    10021 ttttcatatg atgatgtggc acgtttgtat gaagaaacag agaaattatc actgcctctt
    10081 gatgccatct cagaaggaac agtttatcaa atccaatcgg accaagaaag tctttatgcc
    10141 aaattcaatc cagctttgac ctttgttcca gtggactttg aagacttatc tagtcaaatg
    10201 acctacaaca aatgcgtgac tgcctttgct caagaaccct tggatgcagc cattcagaag
    10261 atttctccag aattgtttga ccaatatgaa atctttaaat cacgtgaaat gttgctagaa
    10321 tggtcaccaa agaatgttca taaagcaaca ggtttggcaa aactaatcag ccatcttgga
    10381 atcgaccaaa gtcaagtgat ggcttgtggt gacgaggcca atgacctctc tatgattgaa
    10441 tgggcaggtc ttggtgttgc tatgcaaaac gctgttcctg aagtaaaggc agccgcaaat
    10501 gtagtgacgc cgatgaccaa cgatgaggaa gctgtcgcct gggctatcga agaatatgtg
    10561 ctaaaggaga actaagatat gggattgttt gaccgtctat tcggaaaaaa agaagaacct
    10621 aaaatcgaag aagttgtaaa agaagctctg gaaaatcttg atttgtctga agatgttgat
    10681 cctaccttca cagaagttga ggaagtttct caggaagaag cagaggttga aattgttgaa
    10741 caagctgtgt tccaagaaga ggaaatccaa gacacagttg aagaaagtca ggattcagag
    10801 ccagctgtag gggtttctca agaagaaata gaagaattac caaactcaga agaattacca
    10861 gaggaagaga agcttgagca cgaaggaact gtagaagaaa ataattctga agtgcttgaa
    10921 ccagaagctc ctcaaacaga agaaactgtt caggaaaaat atgaccgcag tcttaagaaa
    10981 actcgcacag gtttcggtgc ccgcttgaat gccttctttg ctaacttccg ctctgttgac
    11041 gaagaatttt tcgaggaact ggaagaactg ctgattatga gtgatgttgg tgtccaagtc
    11101 gcttctaact taacggagga actacgttac gaagccaagc ttgaaaatgc caagaaacct
    11161 gatgtacttc gtcgtgtcat cattgagaaa ttggttgagc tttatgaaaa ggatggtagc
    11221 tacgatgaaa gcatccactt ccaagataac ttgacagtta tgctctttgt tggtgtgaat
    11281 ggtgttggga aaacaacttc tatcggaaaa ctagcccacc gctacaaaca agctggtaag
    11341 aaggtcatgc tggttgcagc agataccttc cgtgcgggtg cagtagctca gctagctgaa
    11401 tggggccgac gagtagatgt tccagtagta actggacctg aaaaagctga tccagccagc
    11461 gtggtctttg atggtatgga acgtgccgtg actgaaggta tcgatattct catgattgat
    11521 actgctggtc gtctgcaaaa taaggataac cttatggctg agttggaaaa gattggtcgt
    11581 attatcaaac gtgttgtgcc agaagcacca catgaaacct tcttggcact tgatgcatca
    11641 acaggtcaaa atgccctagt acaggccaaa gaattttcga aaatcacacc tttaacggga
    11701 attgttttga ctaagattga tggaactgct cgaggaggtg tggttctagc cattcgtgaa
    11761 gaactcaata ttcctgtaaa attgattggt tttggtgaaa aaatcgatga tattggagag
    11821 tttaactcag aaaactttat gaaaggtctc ttggaaggtt taatctaatc agaagcaaaa
    11881 atcctgcaag gcataaactt gcaggaaatt tttttattct aagcgaccat cttgacgata
    11941 ggtgatatct ggttgccaag tccatttggc accgaatttt tcaagtaggt caaagctggc
    12001 ttgaggtccc atgcttccag ctttatagtc atgaagtggg gcaccatttt cagcccagag
    12061 cttttcaata cggtcaatca acttccatga cgcacaaact tcatcccagt ggctaaagtt
    12121 agttgagttg ttatttagga catcataaat caatttttcg tatggttctg gagaagcacc
    12181 agttgcagtc gcatctgtac ggtaatcaag tgagttagga gccaagttaa attcttctcc
    12241 tacttgcttc ccatttaggc taagagagaa gccttctgtt ggttgaatat agatggtcaa
    12301 aatatttgga gcaagtggtt ctccaaagat agaatccatt tgtttaaaga cgatgttgac
    12361 atgggttcct ttttcagtca gtcgtttacc tgtacggaaa aagaaaggaa caccacggaa
    12421 tcgatcgctg tctacaaaga aggcaccaga tgtaaaggtt tcagttgttg attctggatt
    12481 cacatttggc tcgctacgat aagagatgta tttcatgcca tcaatcttac cagagcggta
    12541 ttgcccacgg ataaagtgtt ctttgagttc ttcatcagtt ggatgataga ggtttttaaa
    12601 gaccttaatc ttttcagcac gaatctcgtc ttttgtgaag cttgctggtt tgtccatggc
    12661 gaggagcgaa agaagttgta gagtgtggtt ttggaccatg tcacggaggg caccggattg
    12721 gtcatagtag ccaccacgtt cttctacacc caagcgctcc gcaaaggtaa tttgaacatt
    12781 gtcgataaaa tccttgttcc aaacgttttc aaaaatcaag tttgcaaagc gaactgcaaa
    12841 gatgctttgg atcatttcct taccaagata atggtcgata cggaaaattt gttcttcgtc
    12901 aaatgttgct aggagttcgt cattcaactt gcttgcagtt gcgtaatctg taccaaatgg
    12961 tttttcaacg atcaagcgct caaaaccttt gccatcgaca atgttttcag atttgaggtg
    13021 tttggcaatg gttccaaaga actgaggtgc catagacaag aagaagagct tattgtgttc
    13081 agcttggtat ttttcattaa gctcagcttg taattgacgc aaagcaatat aatgttccga
    13141 atcattgaca tcatggcttt gatagtagaa gtggctagca aattcttggg cttgctcggt
    13201 actatctgcc aaatcaagga tggactcgac aactacagat tcaaaatatt ccttactcca
    13261 aggtctacgg gcagttccaa taacggcaaa gtgcttggaa agattgccgg attgatatag
    13321 tctaaaaagg gaagggtaga gcttgcgttt agccaggtct ccactcgcac cgaaaattgt
    13381 aacaataacc ttagatgaca tctagctacc taatttctat ttttattcct agtcttgcta
    13441 ggtatgagga aacctcattt cataaactta attctaacat aattttccga aaaatcaaaa
    13501 attccaatac gaggtagaaa aactgcaagg aaatccttac agtttgagtt tagacgttta
    13561 agaccttatc taagaactct ttcagacgag ggtgttgtgg gttatcaaag atttggtcag
    13621 gtgttccgtc ttcaaggaac tcgccatctg cagtaaagat aacgcggttg gcaacctgac
    13681 gagcaaatcc catctcatgg gttacgataa tcatggtcat gccttgctca gccaattcct
    13741 tcataacgtt aagtacgtct ccaaccatct cagggtcaag ggcagaagtt ggttcatcga
    13801 agagcatgat gtctggattc attgctaggc cacgagcgat ggccacacgt tgtttttgac
    13861 cacctgatag gctatctgga ttggcattag ctttatctgc tagtccaacc ttttcaagca
    13921 actccattcc caattcctca gcttcttcct tagtcatcaa cttgtgctca ataggagcaa
    13981 aggtgatgtt gtccaataca gacatatgag ggaagaggtt gaagtgttgg aataccatgc
    14041 cgatattttc acggacgtgg tcaacattgg ttgttttttc agttaaatca tagccgttca
    14101 cagtgatgtg accgctagtg acttcttcta aaagattgag gctacggagg aaagttgact
    14161 taccagaacc tgaaggaccg atgatacaaa caacatctcc ttcatagaac ttagtcgtaa
    14221 ttccttttag gacttcattt tttccatagt gcttgtgtaa atcatttaca tcaattttta
    14281 attttaccat taacgaatcc tcttttctaa gcgtttcgct agtctagtca aaagtgtgat
    14341 aattacaaga tagaagatag caaggattgc atacatcttg aaactttggt agttacgagc
    14401 aatgataatc ttaccagttt ggaagagttc aaccaaaccg atagcagata cgatagttgt
    14461 atctttaaga gcgataacga attggttgac aaagtttggc aacatcaatt tagttgcttg
    14521 tggcaagata atcttacgca tggtttttcc ataagagata cccaagcttc ggctggcttc
    14581 catttggcca actggaacgg cctgaatacc accacgaacg atttcagcga tataagcagc
    14641 cgcattgagt gagagggcaa tggttccagc tacaaagtcg ttaattgggc tttgttggcc
    14701 tgtgatagac tcgatgaagt ttggaattcc ccagaagatg aaggctgcaa gaatcatcaa
    14761 tggaatacca cgaataacgt caacgaaaat ctcagagatg acgcgaagag atttgtatgg
    14821 gctaacgcta aacataccga agataattcc gatgacaatg gcaatagcaa atgagataag
    14881 agctagagca agagtgatac caagaccgct aaggagttgt ttgtagttgt tttgaagcaa
    14941 gccccagagc gttgtttcgt caacagtact tgttgaagca gttgaagatt cgctagctag
    15001 gtatttgtca agaatctttt ggaattcacc gtttgcttta aggtttgcaa gtccgttgtt
    15061 gaacatttca atcagttctg gatttgctcc ttttttaacg gcaaaggctg tttcaccgat
    15121 tggagttcca gagattggag ttttcaattt ttgaccttgg ctgatagaat atttgagaac
    15181 aggttcatca tccataacgg catcaatggc accagtgttt aaactgtcat acattgaaga
    15241 accatcagca aaggttttga ttttgtagcc gtatttgctt tgattttctg ttaggaaggt
    15301 ttgagaagca gttccgtttt taacaccgac tgtctttcct tttagatctt cataagaagc
    15361 aatattgctt gattctttga caccaagaat ggtattagca gtgtagtatg attctgagaa
    15421 gtcaaaagtt gccttacgag catctgtgac agacatacca gcgatgatac catcggcttg
    15481 accagcttgg acagcactga tagcagcatc aaaaccaggg ttggtgattt caatttcaaa
    15541 accttggtct ttagcgattg ccttaatcaa ttccatatca ataccagtgt attggttgct
    15601 tgaattttgg aaaacaaaag gtgcaaaaga agaatcgctg gcaatgatat atttagcctt
    15661 aacaggaata gcttttaatc ctgcgagagt agttgtagtt ggcactgctg aagatgatga
    15721 agcagtccat ttcttgataa ttttatcaag actaccatct tttttcattt cagacaaggc
    15781 ttggttaaat tcagtaacca ggtgctcgta tttacttcct tttttcacac cgaaagcaaa
    15841 acttcctaca gcttcaccat ccatttcaat atggaggtct tgaccttggt taatggcata
    15901 ttcgataaca ggtttgtcat ccatcatggc atcgatggca ccagcactca agctgttgtt
    15961 cattaaatca ccagtgtcaa atgttttaat agtaaagccg tatttatctt tgattgtttc
    16021 aaggaaacgt tgagcggcag ttccgttttt aacaccaacg gttttgccag ttaattggtc
    16081 gtacttgcta attttgtgtg actttgtagt agcaatgaca acttttgtat catagtaagt
    16141 atcagacatg gtgaagactt tttcacgttc tttagtcttt gtcatccctg ccatgatagc
    16201 gtcggcttgc ccagcttgaa ccgcattgac tgctgcgtca aatccaggat aggacatctg
    16261 aatgttccag cctttaatct cagcgacttt gttaataatg tcaacatcaa ttcctttata
    16321 agtttgatct gaatctttaa actcaaaagg tgcataggcg gtatcagaaa caatcttaat
    16381 cgtttctgct ttggcaatac ctaatgagaa aattgggaat aaaattagca aaaatgctag
    16441 aaattttttc ttcattttta gtctcctttt ccgaatattc tctcattata tcagaaaaag
    16501 taaaaaaagg caaattttta tatttttcgt gtcagaaaat ataacatcga ttttttcctt
    16561 tccttgaatt gctattaaaa agtgctataa taatagtata tatagaagaa aagaggacgg
    16621 ggatatgaag aacaaaagaa tatttaaaga cttccaagct tcaaaaatga gtttaaacat
    16681 ttacacaagc cccttgttag cctttgtttt tgtcttcata ggagagtttg tggcttttac
    16741 tttgtatggt attggcttgt tagctctcat cggacttgct agaaattttg gagaggctgg
    16801 tcaaaatctt gcaagctact tgcagacctt gcatcagagc ttgacggata aaacaagtga
    16861 ctttcgttta attttaggat tactggcctt tggttttatt cttaacactg tgttcagatg
    16921 gacaagaaaa gttgagaaaa gacctattcg aaccttggga ttttatagag agaatttcct
    16981 cagcaatctt ctgaaaggat ttagtctagg cctggcactt tttcttctga ccttgttagg
    17041 tttagtggtc ttaggtcaat atcgtttgga atccattcac ttgaatcctt attctcttgc
    17101 ctttgtcgtc tttactatcc cattttggat tttacagggg acagcagaag aagtggtggc
    17161 ccgtgcttgg ctacttcctc aattggcctc aagaaccaat ctaaaactag ctattcttat
    17221 atctagcctg ttctttaccc tgcttcatat gggcaattct ggtctcaccc ctctatctct
    17281 agtaaatctc tttttattcg gagttgccat ggctctttac cttctcaaaa ctgatacagt
    17341 ttggggtgtt gcaggtattc atggtgcttg gaattttgct cagggtaatc tctttgggat
    17401 tttagttagt ggtcaaccgt caggaacgtc tctgatgacc tttttaccac aaggcaatca
    17461 agattggcta tcaggtggtt cttttggcat agaaggttcc attatgacaa gtctggtatt
    17521 actactgctg attgtctatc ttgctaataa attaaagaaa gaaaatgaaa ggatgtgact
    17581 tcggtccgtc cttttcttcg tgaaaatact ataagtatgc taaaatagga atagcacatg
    17641 gagagaggat tcttatgatc aatcacatta cagataatca atttaaacta gtatcaaaat
    17701 atcaaccatc aggagatcaa ccccaagcta tcgagcagtt ggtggataac attgaggggg
    17761 gagaaaaagc tcagattctg atgggggcga ctggaacagg gaagacctat actatgagtc
    17821 aggtcatttc taaagtcaat aaaccaactc tggttattgc ccacaataaa actctggctg
    17881 gtcagctcta tggggagttt aaggaatttt tccctgaaaa tgcagttgag tatttcgtat
    17941 cctactatga ttattaccag ccagaggcct atgtcccttc tagcgatacc tatattgaga
    18001 aggatagttc tgtcaatgac gagattgaca agcttcgcca ctcagctacc tcagcccttt
    18061 tggagcgtaa tgatgttatt gtcgtggcct cagtctcttg tatctatggt ttgggttcgc
    18121 ccaaggaata cgctgatagt gtcgttagtc tccgtcctgg tctagagatt tctcgtgata
    18181 aactcttgaa tgacttggtc gatattcagt ttgaacgtaa tgatattgat ttccaacgcg
    18241 gaagatttcg cgttcgtggg gatgtggtag agattttccc agcttcccga gatgaacatg
    18301 cctttcgagt agaatttttt ggagacgaaa ttgaccgtat tcgtgaagtt gaggctctga
    18361 caggtcaggt gttgggagaa gtggatcatt tagcgatttt cccagcgaca cactttgtga
    18421 ccaatgacga ccacatggaa gttgccattg caaagattca ggccgagttg gaagaacaat
    18481 tagctgtctt tgaaaaggaa ggtaaactgc ttgaagccca gcgtttgaaa cagcggacag
    18541 agtatgatat cgaaatgttg cgtgagatgg gctataccaa tggggttgaa aattattctc
    18601 gccacatgga tggacggagc gaaggagagc ctccttatac gcttctcgac ttcttcccag
    18661 atgatttctt gattatgatt gacgagagtc atatgacaat agggcaaatc aagggcatgt
    18721 acaatggaga ccgttcgcgt aaagaaatgc tggttaatta tggtttccgt ttgccgtctg
    18781 ctttggacaa tcgtcctctc cgtcgggagg agtttgagag tcacgttcat cagattgttt
    18841 acgtttcagc gacacctggt gactatgaaa atgaacagac cgagacagtg attgagcaaa
    18901 tcattcgtcc aacgggactc ttggatccag aggtggaagt ccgtccgact atgggacaga
    18961 ttgatgacct cttgggtgaa atcaatgccc gcgttgaaaa aaatgagcgt acctttatca
    19021 caactttgac caagaaaatg gcagaggatt tgaccgacta cttcaaggaa atgggtatca
    19081 aggtcaagta catgcactcg gatatcaaga ccttggaacg gacggagatt atccgtgacc
    19141 tgcgcttggg tgtctttgat gtcttggtcg gaattaacct gctccgtgaa ggaattgacg
    19201 ttcctgaagt gagcctcgta gctattctcg atgctgacaa ggaaggtttc cttcgcaacg
    19261 aacgtggact catccagact attggacgtg ctgctcgtaa cagtgaaggc catgtcatca
    19321 tgtatgcaga cacggttacc cagtctatgc aacgtgctat cgatgaaact gcccgccgtc
    19381 ggaaaattca gatggcctat aatgaagagc atggtatcgt accacagaca atcaagaaag
    19441 aaatccgtga cctgattgcc gtgaccaagg cagttgctaa ggaagaggac aaggaagtcg
    19501 acatcaatag cctcaacaaa caagagcgca aagaactcgt caagaaacta gaaaaacaaa
    19561 tgcaagaagc cgtcgaagtg cttgactttg aactggcggc tcagatccgt gatatgatgt
    19621 tggaagtcaa ggccttggat taggggaata gtatgattta tttaagaaag ttaaagagag
    19681 aagatttgat gtctttatgg gaaatggctt attcacaact taatccagtt tggaaacagt
    19741 atgatgctcc ctattatgat gattatcagt atttttcaaa ttttaaagaa ttcgaactac
    19801 aaaaatcaga atccatttta agcaactcaa atcgccttgg tatttttgtt gatgataaac
    19861 tagttgggac tgtttcgcgt tattgggtat gtaaacaaac aagatggatg gaattgggaa
    19921 ttggtattta tgataaaaaa ttctggaaca ctggtattgg gaaagttgct atgttgcagt
    19981 ggatagatag gacgtttcag gattacttgg agttggagca tctgggtttg acaacttggt
    20041 cagggaatat tggtatgatg aaacttgctg aaaaattaag aatgaaaaaa gaagctcata
    20101 ttccaaaagt tcgttattat caaggtaaat attttgatag tattaaatat ggtattttga
    20161 gagaagactg ggagaaaata aatgacggtt attatcaaat caatggaaac tcctaaagag
    20221 atagaaggta aatcccttgt tcactggcaa acgtggagag agtcttatga tgaccttttg
    20281 cctgcggaat ttcaggatac aatgacatta gaaagatgtc gattctttag tcaaaagtat
    20341 ccagaaaata cattgattgc gatggatggc gtgaaggtgg ttggttttat cagttatggc
    20401 aattttcgtg atgagactat tcaagctggt gaaattattg ctttatatgt tttaaaagac
    20461 tattatggaa aaggtatcgc acaaaagtta atgaaagcag ctttgactgc tcttaatcat
    20521 ttttctgaaa ttttcttatg ggtattgaaa gataacaagc gcgccattgc tttctatcaa
    20581 aaaatgggtt ttacttttga tggacaagaa aaaatacttg aacttggaaa gcctataaag
    20641 gaaaaacgaa tggtgttcta ttctaaataa ttttaaaaaa gtaaaagata atatctctgg
    20701 tagtaagtct gaaaatttaa taaattagaa agcgagtaaa tttatgtccc gttcccaatt
    20761 aacaatttta acaaatatct gtctgattga agacctcgaa actcagcgcg tggtgatgca
    20821 gtatcgcgcc cctgaaaaca atcgctggtc tggttatgcc tttcctggag gtcatgtaga
    20881 aaatgatgag gcttttgcgg agtctgtcat tcgtgaaatc tacgaagaaa cagggttgac
    20941 tatccaaaat cctcaacttg tcggcattaa aaattggcca ctagatacag gtgggcgcta
    21001 tattgtcatt tgttataagg cgactgagtt ctctggtacc cttcaatctt cagaagaggg
    21061 agaagtttct tgggtgcaaa aagaccagat tccaaactta aatctggcct atgatatgct
    21121 accattgatg gaaatgatgg aagctcccga caagtcagag tttttctatc ctcgccgtac
    21181 agaagacgat tgggaaaaga aaatcttcta gtcttttact aaataaccta gctgatccaa
    21241 ggcctcctcg atatagtgga ggtcttgttg tgtttcggct tcgactaggt ggtaatggat
    21301 gccatctgtc aattcagata gaggtctaaa gtcagaatgc tcgacttgtt ctagaaaatg
    21361 ttgcacatct ctgcgacaag acagtttaag caaggtttca atttctccat aaacgggatg
    21421 gtcaatcaag gtattctgga catgcccacc attatcgaca atagcaagga gttcctgacc
    21481 gatttcttca acttcatgtt tcactttgaa aagtttgtga acataaggat tggtatcaat
    21541 ttgcttatag atgtagccac gattggtgga taggatagga gcgccatcgg ctctcaaaat
    21601 tgcaatgtcc tgcacaatga cctgacgtgt gacatgaaaa tgttccgcca aactttggcc
    21661 attaagggct ttaggagctt ctttcaagag ttggagcagg gcttgtttgc gatcttttgt
    21721 catagctttt ccttttaacg gcgttttcga agcactttat agacagctag tgctaatgta
    21781 tagtctacca tactatggat aattgtgcca aatccaacta gtacaaatag aacatagaac
    21841 atattttcta cattggtacc cgaagttgcg taaaaaagga cacaggctaa tacttcagca
    21901 agggcatgaa caacagccaa aacaaaattg aaaacccagg aagattttgg tttatctagg
    21961 gtatcgggga atttttgtag gtaaagagct cctaaagcac caaaagatat atgggaaaaa
    22021 gcccgaaaaa cgataaccat gggatagcca gccatcaaaa atccaaaact agaggctagg
    22081 atgacaaaaa ctgccatcaa gggcgacaag aacatggcta taaaaatagc gatgtggctc
    22141 cccaaagtat aggaagcagg tggaatgaca atcttgaaag gcataacaat tggaatcaaa
    22201 atcgcaatag ccgttaaaag ggctgtcatt gtcataaatt gtgtcttttt ccgtgtattc
    22261 acaagaatct cctttttaac tgcatataca ctagtatggt acaataaacc agacaataaa
    22321 gcaagaattt acttgggttt atagatcatt ttttagttaa aagttatagt agattgaaac
    22381 tagaatagtc cacctctact tctaaaacat tgttagaaat cgatttgact gtcctgatcg
    22441 atttgtcctg ttcttatttc gttttactat agtaaagatt tcattaaaaa gaaactgtat
    22501 agagcaaaat ctccaccttc aggtttgaaa gcggagattg tttttatttt ttcaaggttt
    22561 gtagtcgtgg aacaatggct agggttagaa ttgcagaaat gaccaattca gcaattgaat
    22621 ttgttgagat aacggttgcc agaagaagtt ggatatttcc attataaaca tttccaaata
    22681 ggaagaagat tcctccaagg acaaagatag tatttgtcaa ggaaccaaga gccccagcta
    22741 aaatcagacc agtcttgttt ttcatcagtt tatagactaa gtaaggagtt aaaccaatca
    22801 aaatacgtgg gacaatggca atgatagctg agtagatgtt tccgtttggt acgaagggag
    22861 agaagaggta gcttgtcggt agaatcgtaa tcgtgttaac cgtcaagcta agtaatccca
    22921 tcaaaaatcc aagtgtaacc ccaactcgtg gaccataaat aatgctggca ataatgacag
    22981 gaatatgaac aatggtcggt ttgattggaa atggaaaaag gttaaagata agtgagctca
    23041 gaaagtgtat cacgagcatg gtagcaaaaa agatagcaat gggtgcaata ttagagcgtt
    23101 ttttcatcga tagtttcctt tattctttct aaaataattg tgaggtcagc taaagctcct
    23161 cgtccgtggt ctccacaagc tagtagggat tccttaggag caatcagctg atagccgtag
    23221 gtttctaatg ttttcagatt attctgagtt actggatggt catacatttt tgtattcata
    23281 gcaggagcta ttagtttggg aatatgactt ggtagggcta gagctgtact ggttaccatg
    23341 ttgtccgcaa atccgtgagc tagttttgca atagtgttag cagttgcagg taccacgata
    23401 aataaatctg ctttttttcc aagttcgata tgattgacct gatcaggata gggttctttc
    23461 atgacatcca agtggacagg attctgtgag agtacctgta gtgtcaaagg ttggataaac
    23521 tctgtagcag cctgagtcat taagacagtg acttgatggc cttgtttttt tagagaactg
    23581 actaaatctg ccgacttata agaggcgatt gaacccgtta cagccaagag aatgtttgcc
    23641 atagttttcc tttctaagaa tgataggctt gaattttttc aaggaggagt tctgcaattt
    23701 cttctttagt ctggactgtt tgaagctgat ttttctcaac aaatatagct cggtgctgac
    23761 ctgctgaaat ttgagtcagg tcattcgcga tgattaaatc tgcttgattc ttgataagac
    23821 tttttcgtgc aatgtcaacc agatgatctt cggtaacatc aaccagcagt ttgaaaccaa
    23881 tcagatgaat agtaggattc cattccttga ctagggatat gattttgggt gtctttttaa
    23941 ggaacaaaac ctgaacctca tcagttgaag aaatcttggc ctgatgattc tgcttgctta
    24001 aaaattcttt tagattggag ctagcctgaa cttcctcaag ccctgtcata taaacaggag
    24061 tgtagtcaga aacagccatt gagtggatca agacctgata atcctgaaca cgttcttgca
    24121 tttctattag aaggtccttg gtattggtaa tttctcgaat acttaggtta ggatgaggct
    24181 ctggcttcag agctcgtttt gtcgtaatta aacaaacttc atgccctgca gaaagcaaag
    24241 tctctgtgat aattttcccc aagtgacctg tagaatggtt agtgatagag cggacgctat
    24301 cgatagcttc actggtaccg cccgatgtaa ctaaaatttt catagcacta ttgtacacaa
    24361 aaataagcgg taagtaaaat gaaagtgata aatttttggt aaaaacgaag atttactata
    24421 gtttcaaact ataaagccat ataaaattta agaaaggact ctaaaagtct taaaaacaga
    24481 gatatttacg aacgtttaga gagttttaag atgttaaaaa tcttgttttg tgatataatg
    24541 aattatcata taagaggtta gaggaatttt gaatgaaaac agatattgaa atcgcacaga
    24601 gtattgagtt gaagccaatt gttgatgttg tagagaaact tggtatttct tacgacgatt
    24661 tggagttgta cggaaagtac aaggctaaac tcagctttga taaaattcgg gcagttgaga
    24721 gcaatccagt cggtaaattg atcttggtta ctgccatcaa cccaacacct gcaggtgaag
    24781 gaaagtcgac gcttaccatt ggtcttgcgg atgccttgaa caagattggc aagaaaacca
    24841 tgattgctat ccgcgaaccg tctcttggtc cagtcatggg gatcaagggt ggtgctgctg
    24901 gtggtgggta tgcacaagtt ctgccaatgg aagacatcaa cctccacttt actggagata
    24961 tgcatgctat tacaactgcc aacaatgccc tttctgcctt gattgacaac cacttgcacc
    25021 aagggaatga gctgggaatt gatcaacgtc gtatcctctg gaaacgcgtt gtggacttga
    25081 acgaccgtgc gctccgccat gtgactgttg gtcttggtgg tcctctaaac ggtattccac
    25141 gtgaggatgg ttttgatatt acagttgctt cagaaatcat ggcaattctt tgcttggcaa
    25201 cggacatcga ggacttgaaa cgtcgtttgg cgaatatcgt tattggttat cgctatgacc
    25261 gtacgcctgt ttctgtaggt gatttgcagg ttgagggtgc cttggctttg attttgaagg
    25321 atgctattaa gccaaacttg gttcagacaa tttacggtac acctgccttt gtacacggtg
    25381 gtccatttgc caatatcgct catggctgta actctgtttt ggcgacgaca acagcccttc
    25441 acttggctga ttacactgtt actgaagctg gttttggtgc ggaccttggt gctgagaaat
    25501 tccttgatat caagacacca aacttgccaa catctccaga tgcagttgtt attgtcgcaa
    25561 cccttcgtgc ccttaagatg aatggtggtg tggctaaaga cgctctgact gaagaaaatg
    25621 tagaggcagt tcgtgcaggt tttgctaact tgaaacgcca cgttgaaaat atccgtaagt
    25681 tcggtattcc agcagttgta gctattaacg aatttgtatc tgatacagaa gctgaaattg
    25741 cagtcttaaa agaactctgt gcctcaatcg atgtaccagt tgaattggct agtgtctggg
    25801 ctgatggagc agaaggtgga gtagcacttg ccgaaacagt tgttaagaca attgctgaaa
    25861 atccagctaa ctataaacgt ttgtacgata atgacctttc tgtccaagaa aagattgaaa
    25921 aaattgtcac tgaaatctat cgtggtagca aagtgaactt tgagaagaaa tctcaaacac
    25981 aaattgctca aatcgttcaa aacggttggg acaaattgcc aatctgtatg gctaaaactc
    26041 aatacagttt ctcagacaat ccaaatgcgc ttggagcacc agaaaacttt gaaattacca
    26101 ttcgtgaatt ggtaccaaaa ttaggtgcag gcttcatcgt tgccttaact ggtgatgtta
    26161 tgaccatgcc aggtcttcca aaacgtccag cagctctcaa catggatgtt gaaagcgatg
    26221 gaactgtact aggcttgttc tagtggattg aaattgactt taagtgagtt tggaaataat
    26281 atccaagctc attttttctc agactgcaag cagttcatca ataagattta tactcttcga
    26341 aaatctcttc aaactacgtc agcttcacct tgccgtaggt atatgatact gactctgtca
    26401 gttctatcca caacctcaaa acagtgtttt gagcaacctg cggctagctt cctagtttgc
    26461 tctttgattt tcattgagta taagtagata gaaaagttat atactatcta ttgaaaatat
    26521 tataaatcag atacaaagac aggtgaaacc ttctgtgtta tactagagat atgttagatt
    26581 tgaaagaata cggtatcgtc atgtggccgg aggagaaagt catttctttc cgtgagaaac
    26641 ttctcgcttg gtatgatgaa aacaaaagag atttgccttg gaggagaagt aaaaatcctt
    26701 atcacatctg ggtatctgaa atcatgcttc agcagaccag ggtggataca gttatccctt
    26761 actacgaaag attcctggac tggtttccaa ctgtcgaaag tctggcaact gcgcctgagg
    26821 agagtttact gaaagcttgg gagggcttgg gctattattc tcgagttcgc aatatgcagg
    26881 ctgcagccca gcagattatg actgactttg gtggccaatt tccaaatacc tatgaaggaa
    26941 tttccagctt gaaagggatt ggaccttaca cagcaggagc catttccagt attgctttta
    27001 acttgcctga gccagctgta gatggtaatg tcatgcgggt cttggcgcgt ctgtttgaag
    27061 tcaaccacga tattgggatt ccaagtaatc gaaaaatttt tcaggcaatg atggagatct
    27121 tgattaatcc ggatcgtccg ggtgatttta atcaagcctt gatggactta gggtctgata
    27181 ttgagtctcc tgtaaatccc agacctgaag aaagcccagt caaggacttt agtgcggcat
    27241 atcagaatgg aaccatggac cgttatccaa ttaaatctcc taagaaaaag ccggttccta
    27301 tttatcttaa agccttagtt gtgaaaaatt ctcaaggaca atttttactt gaaaaaaatg
    27361 aaagcgaaaa gctcttggca ggtttttggc atttcccctt tatagaagtt gataactttt
    27421 cgcaagaaga gcagtttgac ctctttcatc aggttgcgga agaaagtgtg aactttggcc
    27481 ccagtccaga agagagtttc cagcaggact atgacctaga tgttgattgg cttgatgttt
    27541 gttttgatac agtccagcat gtttttagtc atcgaaaatg gcatgtacag attgtagcag
    27601 gtcaggtgag tgacttccat gatttttcag atagggaagt tcgttggctt tcaccagaag
    27661 aattcaagaa ttacccactt gccaaacccc aacaaaaaat ctggcaggct tatgcacaag
    27721 ccaacttaga cagtagccaa gactagctat tgtgtcttct gactattttt tggtataata
    27781 gtagagaaaa aggtgaacat atgaaaaaaa tactaattgt agatgatgag aaaccaatct
    27841 cggatattat caagtttaat atgaccaagg aaggttatga agttgtaact gcttttaatg
    27901 gtcgtgaagc gctagagcaa tttgaagcag agcaaccaga tattattatt ctggatttga
    27961 tgcttccaga aattgatggt ttagaagttg ctaagaccat tcgtaagaca agcagtgtgc
    28021 ccattcttat gctttcagcc aaagatagtg aatttgataa ggttatcggt ttggaacttg
    28081 gggcagatga ctatgtaacg aaacccttct ccaatcgtga gttgcaggcg cgtgttaaag
    28141 ctcttctgcg tcgttctcaa cctatgccag tagatggtca ggaagcagat agtaaacctc
    28201 aacctatcca aattggggat ttagaaattg ttccagacgc ctacgtggct aaaaaatatg
    28261 gcgaagaact agacttaacc catcgtgaat ttgagctttt gtatcattta gcatcgcata
    28321 caggtcaagt catcacgcgc gaacacttgc ttgagactgt ctggggttat gactattttg
    28381 gtgatgtccg cacagttgat gtgactgtac gacgtctgcg tgagaagatt gaagatacgc
    28441 ccagccgacc agagtatatc ttgacgcgcc gtggtgtagg gtattacatg agaaataatg
    28501 cttgatttac tgaaacaaac catttttacc agagatttta tctttatcct gattttgtta
    28561 ggtttcatcc ttgttgtgac cctcttatta ctggaaaata gacgtgataa tattcagttg
    28621 aagcaaatca atcaaaaggt taaagatttg attgcaggag attattccaa ggttcttgat
    28681 atgcaaggtg ggtctgaaat caccaatatt accaataatt tgaatgactt gtcggaggtt
    28741 attcgtctca ctcaggaaaa tctagaacaa gagagtaaga ggctaaatag tattctgttt
    28801 tatatgacag atggggttct tgcgactaac cgtcggggtc agattatcat gattaacgat
    28861 acagccaaga agcaactggg gttggttaag gaagatgttc tgaatagaag cattttggaa
    28921 ttgctcaaga tagaagaaaa ctatgaattg cgtgatttga ttacccaaag tccagaattg
    28981 ttgctagatt cccaagatat caatggcgaa tatttgaacc ttcgagttcg ctttgccttg
    29041 atacgtcgag agtctggctt tatttcaggt ttggtggctg ttttgcatga tacgacggag
    29101 caggagaagg aagaacgcga acgaagactc tttgtttcca atgttagcca tgagttacgg
    29161 actcctctga ctagcgtaaa atcctatctt gaagccttgg atgagggggc tttgtgtgaa
    29221 actgtagcac cagactttat caaggtttct cttgatgaga ccaaccgtat gatgcgcatg
    29281 gtgacggatc tcctccatct ttcacgtatt gataatgcta ccagtcacct agatgtggaa
    29341 ctgattaact tcactgcttt tattaccttt atcctcaatc gttttgacaa gatgaaagga
    29401 caggaaaagg agaaaaaata tgagctggtg agagattatc ccatcaattc tatctggatg
    29461 gaaattgata cagataagat gacgcaggtt gtcgacaata ttttaaataa tgctattaag
    29521 tattcgccag atgggggtaa aatcactgtc agaatgaaga caactgaaga ccagatgatt
    29581 ttatccattt ctgaccacgg tttggggatt cctaagcagg atttaccacg tatctttgac
    29641 cgtttctatc gtgtggatcg tgctagaagt cgtgcacaag gtggtacagg tctaggactg
    29701 tctatcgcta aagaaattat caaacaacat aagggcttta tttgggccaa gagtgaatac
    29761 ggcaagggtt caacctttac cattgtactc ccttatgata aggatgcagt gaaagaagaa
    29821 gtatgggagg atgaagtaga agactagaat gagtgaaata ggctttaaat acagtatttt
    29881 agcgtcgggt tccagtggaa attcttttta tctggaaacc tcaaaaaaga agcttttagt
    29941 agatgcaggc ttgtctggca agaaaattac cagtctgcta gctgaaatta accgtaagcc
    30001 agaagacctg gatgccatct tgattaccca tgagcattca gatcatatcc atggagtagg
    30061 cgttttggct cgcaagtatg gtatggatct ttatgccaat gaaaagacct ggcaagctat
    30121 ggaaaatagt aaatatcttg gcaaggtgga ttcttcgcaa aagcatattt ttgaaatggg
    30181 taaaaccaaa acctttggag atatcgacat cgagagtttt ggtgtaagcc atgatgcagt
    30241 cgcaccgcag ttctatcgct ttatgaagga tgataagagt tttgtcctct tgacagatac
    30301 aggttatgtc agtgaccgta tggcgggcat tgtcgaaaat gcggatggtt atcttatcga
    30361 ggccaaccat gatgtagaga ttttgcgatc aggttcttac gcttggcgac tcaaacaacg
    30421 aatcctatct gaccttggtc acctttctaa cgaggacggt gctgaagcta tgattcggac
    30481 gctaggaaat cgtactaaga agatttacct tgggcattta tctaaggaaa acaatatcaa
    30541 ggaactggct catatgacca tggtcaatca gctggctcaa gctgatctgg gagtcggagt
    30601 agactttaag gtttatgata cctcaccaga taccgcaaca ccattgacag agatatagaa
    30661 agaacgctga gaaggtgttc ttttt
//
LOCUS       DAVKQB010000026        30510 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKQB010000026 DAVKQB010000000
VERSION     DAVKQB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.026"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(4..300)
                     /locus_tag="VW528_001157"
     CDS             complement(4..300)
                     /locus_tag="VW528_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9911981.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            complement(284..2443)
                     /gene="pfbA"
                     /locus_tag="VW528_001158"
     CDS             complement(284..2443)
                     /gene="pfbA"
                     /locus_tag="VW528_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9911982.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYSGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVVN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKINNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3573..4799)
                     /locus_tag="VW528_001159"
     CDS             complement(3573..4799)
                     /locus_tag="VW528_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9911983.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFKNEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4842..5534)
                     /locus_tag="VW528_001160"
     CDS             complement(4842..5534)
                     /locus_tag="VW528_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9911984.1"
                     /translation="MLKWEDLPVEMKSSEVESYYHLVSKRKGSLIFKRCLDWFLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(5707..7458)
                     /locus_tag="VW528_001161"
     CDS             complement(5707..7458)
                     /locus_tag="VW528_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911985.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDDQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(7448..9193)
                     /locus_tag="VW528_001162"
     CDS             complement(7448..9193)
                     /locus_tag="VW528_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9911986.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLVFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9321..9851)
                     /locus_tag="VW528_001163"
     CDS             complement(9321..9851)
                     /locus_tag="VW528_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9911987.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     PEACRSVGLSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDG
                     VIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKPGVLYSS
                     KISSNRVNVALPYVGY"
     gene            complement(9848..10304)
                     /locus_tag="VW528_001164"
                     /pseudo
     CDS             complement(9848..10304)
                     /locus_tag="VW528_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10438..11265
                     /gene="xth"
                     /locus_tag="VW528_001165"
     CDS             10438..11265
                     /gene="xth"
                     /locus_tag="VW528_001165"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9911988.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11277..11375
                     /locus_tag="VW528_001166"
     CDS             11277..11375
                     /locus_tag="VW528_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9911989.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEY"
     gene            11420..11659
                     /locus_tag="VW528_001167"
     CDS             11420..11659
                     /locus_tag="VW528_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9911990.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12042..12137
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12537..13118
                     /locus_tag="VW528_001168"
     CDS             12537..13118
                     /locus_tag="VW528_001168"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9911991.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13118..14380
                     /locus_tag="VW528_001169"
     CDS             13118..14380
                     /locus_tag="VW528_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9911992.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14430..14891)
                     /locus_tag="VW528_001170"
     CDS             complement(14430..14891)
                     /locus_tag="VW528_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEU9911993.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(14888..15652)
                     /locus_tag="VW528_001171"
     CDS             complement(14888..15652)
                     /locus_tag="VW528_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9911994.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYYPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(15833..16237)
                     /locus_tag="VW528_001172"
     CDS             complement(15833..16237)
                     /locus_tag="VW528_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9911995.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVIGQRSEERRVRI"
     gene            complement(16307..17788)
                     /locus_tag="VW528_001173"
     CDS             complement(16307..17788)
                     /locus_tag="VW528_001173"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9911996.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWETYVHYPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVDQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKAFRFAGFEQVKAGIVKWPMSVLRLTSDSKEDLINLADKILQEWRQYSDPAVQ
                     ILAETDRTPHHTITPIARKRDGQFELDLVLRDNQTSAEYPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(17807..18985)
                     /locus_tag="VW528_001174"
     CDS             complement(17807..18985)
                     /locus_tag="VW528_001174"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9911997.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKETVGKHYEEVVGYAPSFYIAEVAGGTHVLD
                     "
     gene            19150..20157
                     /gene="galR"
                     /locus_tag="VW528_001175"
     CDS             19150..20157
                     /gene="galR"
                     /locus_tag="VW528_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9911998.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(20203..21240)
                     /locus_tag="VW528_001176"
     CDS             complement(20203..21240)
                     /locus_tag="VW528_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9911999.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(21241..21594)
                     /locus_tag="VW528_001177"
     CDS             complement(21241..21594)
                     /locus_tag="VW528_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9912000.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(21838..22728)
                     /locus_tag="VW528_001178"
     CDS             complement(21838..22728)
                     /locus_tag="VW528_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9912001.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            22861..23397
                     /locus_tag="VW528_001179"
     CDS             22861..23397
                     /locus_tag="VW528_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9912002.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FIGQEVVQFYLDLLIPKN"
     gene            complement(23563..24369)
                     /locus_tag="VW528_001180"
     CDS             complement(23563..24369)
                     /locus_tag="VW528_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9912003.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(24714..26234)
                     /locus_tag="VW528_001181"
     CDS             complement(24714..26234)
                     /locus_tag="VW528_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9912004.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDSIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(26227..26955)
                     /locus_tag="VW528_001182"
     CDS             complement(26227..26955)
                     /locus_tag="VW528_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912005.1"
                     /translation="MIEYKNVALCYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(26969..27733)
                     /locus_tag="VW528_001183"
     CDS             complement(26969..27733)
                     /locus_tag="VW528_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9912006.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGATYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLKNGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(27737..28180)
                     /locus_tag="VW528_001184"
     CDS             complement(27737..28180)
                     /locus_tag="VW528_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9912007.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(28862..29158)
                     /locus_tag="VW528_001185"
     CDS             complement(28862..29158)
                     /locus_tag="VW528_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9912008.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(29180..30244)
                     /gene="pepA"
                     /locus_tag="VW528_001186"
     CDS             complement(29180..30244)
                     /gene="pepA"
                     /locus_tag="VW528_001186"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9912009.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALLGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         8990 a         6887 c         5517 g         9116 t
ORIGIN      
        1 ttattatttc attttactat actctgttaa ttcatatgag tttaaaccga tttcatcttt
       61 aacctcgagt aaagcagttt caaatatttg tttaagagtt tttgattctt tacaattaac
      121 cgacaaactt tctgataaaa tatgtacaac ttctgagact gaataaccta tctcctcttt
      181 agaattatat aaatctgtag ctccaccaat aatccaaaaa tactgatttt gtgaacctac
      241 aatatcctca ttttctacgg aacttccttg tatcgaacaa attttattta tctttaccat
      301 aatacttcaa cccttttagt gtcaaaagta aaccaattcc tgtcactgtt aagaatagtt
      361 ccataatctt gttcgaacca gtctttggta atttttgttt tacatctact atttctttag
      421 atttattaat atgattttca gtttctctgc catctccaac tattttatag tttacttctt
      481 ctgtcttatt atcttgttta ttgttgatct tgtcattcat ttgtctatta tctttacttg
      541 agttaaactc tccgttcttc tggttactat caattacatt atttgaatta gattgttttt
      601 cctctttgtt tttttctttt tcgtttttat cacttaaatt atttgttaca attttgtaaa
      661 gcccattctc cgttacaata ttgaaattac catcgctatc acgtataaca ggttctttcc
      721 catttgcatt agatttgatg aatgatatat acttaccgga taaattataa aattggttat
      781 ttaaaacggt tattttaccc tttgaatcct caataacaat tccttcttta ccaccgaata
      841 tactattctc tgagactact aaattatcac taactcgtaa taattcaata tttggttgtt
      901 ctgtttcctt agaattatta ttaactacat tttttgttac agtaatatct gatacttttc
      961 ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga tcggcaatat
     1021 taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct ttagtgtttt
     1081 tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca ctctctcctt
     1141 taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct gtaaaacgta
     1201 cacctgaata catcatgtta tcaaaatgat tatttaaaat tttaatatta gaggggttct
     1261 gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca gatttatcac
     1321 ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca tcatcattca
     1381 aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc ttacttatga
     1441 tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa ttatcaacta
     1501 atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc ttgaatgtta
     1561 cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa gaatttataa
     1621 gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata tcaatcgtac
     1681 caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca tcatccgtaa
     1741 ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca tttagaattg
     1801 tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt tcttttacta
     1861 aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct gcagcatcta
     1921 ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctaccgca ccataatctt
     1981 ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt ataacatctt
     2041 ccttataact cttattttca ttagtagatg ttactgtaga ttcaactgtt ctgttgtcta
     2101 attgtgaatt ggaacgaata gtaccattag agctgatact attaacactt aacgaattat
     2161 ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattc tgattaacag
     2221 ttggtgaatt atctaaatca ttagtaattg cattagaaga ctcttttgta gccatcggtg
     2281 aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt gaaattgcca
     2341 atagtaccga gcaagtccct acctttaatt tacgaatact gaatacctca ttaggaacaa
     2401 aatatttcat cagaacctcc aattttttta ctatttttaa caattgatta ttacttttct
     2461 taaaatagat agaatgacac cgctgtcatt atctttatca tatattttcc aaattgaatt
     2521 gtcaaggaat aacacaagaa aatattaaaa tttatactct tcggaaatct cttcaaacta
     2581 ggtcagtgcc gccttgccct aagtctaggt tactgacttg gtcagttcta tagtagacgg
     2641 aaactataat agtacacctc tacttctaaa acattgttag aaatcgattt gactgtcccg
     2701 atcgatttgt cctattctta tttcatttta ctatatctac aacctcaaag cagggttttg
     2761 agctatctat ggccaacttc ctagtttgct ctttagcctt ccattcaaaa gtgatgctat
     2821 ctttcttagc ttgaatataa aatatcccat ttactatatt taaatttgtt gatatgttgt
     2881 aaattataaa tgaaagaaca aataccttgt catacaagca ttcgccaggg atatttacat
     2941 gaatatcgct catatggcgg attgaaacta aaacactaca atatatttct taaatagacc
     3001 ttctataaaa gctagattta acctcgataa aaatagatgc agtgcttcta aaatactgga
     3061 atagggcata taggtaatcg tagacaagtg ttttatagat tgtctcgcgg gagatctaat
     3121 gatgtatagc aagcatcact aaacatatag aatatttgta ttattataag atcaatatgc
     3181 tataaattct atcatgcaaa agaatgacta gaacatgaaa gacaggcaca aaactttgaa
     3241 caatattctg aaaacaaaaa aagacgcgag tttccacaat ctcactcatg gaaactcacg
     3301 tctttcaaaa aatagggtga attgaaaact agaacaagac actagatttt ctaaatgatg
     3361 tctagtaagg attctaaatg ctacaccaaa ttgctcacat cttatttcaa accacgggac
     3421 tctcacaaga aaggaaaatc ctataaaaat tctctctctg aggagaaaga ggcctcaact
     3481 acaggtgtaa actattatca attgaaaaca acagttttta cactcaatta gactttttag
     3541 ggacaggatc actttcgctt gactgtagtt tgtcattttt ttgataaagt tagcactttt
     3601 tcagaaactg ttttgaaagt ctcaatgata tagtctactt cttcatcgct taatttagta
     3661 tgaagaggga gggtaatttc attcttaaag aaggcatagg ccttaggata gttcgtcata
     3721 tcaaatccaa gattcttata ggctgtcaag agaggaagcg gtttgtagtg aacattactt
     3781 gcaattcctg ctttagccaa ttcttggatg atgaggttgc gttcttctag gcttgctcct
     3841 tctacacggg tgatgtagag gtggcgtgaa gattcgacag tttcagtctt gtgtgccaaa
     3901 ggatggatgc gagaacctgc aaaaccacta tcatagcggt ccacaatgtc cttacggcgt
     3961 tgcaacaaac ttggatagcg gtccaattgt accaaaccaa gtgaagccat gatatcggtc
     4021 atgttgcact tataggctgg tgtaacgata tcgtattccc atgaccccag ttgcatcttg
     4081 gcaagagcat ccttagtttg cccgtgaagg gaaaggattt ggaattcctt gtacatctct
     4141 tcgtcatcaa tcactggatt ggctttccaa gtcgcacttc caccttctgc cgttgtaaag
     4201 ttcttaacgg catggaatga gaaggaagta aagtcagcga tagaaccaga aggttgtcct
     4261 ttataagtag atcccaaagc gtgggcacta tcagagacaa tgacaatacg gttaaaggcc
     4321 ttttgccact tgcttgaagc agtaaagaag tcacgttttt tctccacgac ttggaacaaa
     4381 cggtcataat cgcaaacaat ccctgcgagc tctactggga taatcacctt agttttctca
     4441 gtgatagctt gctcaagcag gtcatagtcc atctcaaacg tatctgcttg gatatccacc
     4501 atgacagggg ttgctcccac gtgcgtaatg acactacatg aagctgtata ggtcatggct
     4561 ggaacgatga cttcatcacc aggtcccact tccaaaacgc gtaaaatcaa ctccagagcg
     4621 gctgtcgcag agttgagaca aacagtctta ggtgtctgtg tgtaaagaga caagcggcgc
     4681 tccagttctt ttgttttagg acctgttgtg atccaaccag aacgcagggt atccgctact
     4741 tcagcaattt ctgcttctgt gatatcaggc ggtgaaaatg gaatattgta atttggcatt
     4801 gatttgctcc ttttatctgt actcattttc ttatgactac tttattttag tacctcaaac
     4861 acggtttgaa acatgatttt gatgtcccca aagaaactaa actctcggag ataggcgagg
     4921 ttatagcgca tcttttcagg aagaacatgc tccacatagg cctgatcaac tgacagacct
     4981 ttctccgtca tttgactgat gatggtgtcc tcatccttgt agttgatgct ggctggagag
     5041 gtaattcctg cttgcaagag caaggttgcc atcatttcag ggctatactg ctctgtataa
     5101 cgtggcactt caggtcgtgt accgacaaag gacatctcac ctttaaggac attgaccaac
     5161 tgaggcagtt cgtccaaacg gacacgtcgg atgaaatttc caaccttggt aatacggcta
     5221 tcgttagcag aagtcaccag acttcctttt ttatccgcat ccgtcaccat ggtacggaac
     5281 ttccaaatct tgaaccgacg gttgtactgg gtcacacgct cttgcttgta aatcactggc
     5341 cccttgctat ccaacttgat ccaaatgctc aagatgagaa agatgggaga ggttagaacc
     5401 agtaagacca aggccaaaaa ccagtccaag caacgcttga aaatcagcga acccttcctt
     5461 ttagagacaa gatggtagta agactcaacc tcgcttgatt tcatttccac aggcaagtct
     5521 tcccatttca gcatactgtt ctcctttgtt tttattatac tatttgtcaa catttttcat
     5581 tataccacaa aatggaaaag gcggtgaaag aaatctgtaa tttgaaggat ttccttctag
     5641 tactcaatga aaatcaaagt acagctttga gattgcagat aaagctgaca tggtttaaag
     5701 agattttcaa agagtatcgg ccatggcccc tgcctgcaaa ctatacatct tgtgataggt
     5761 tcctcccaga gtcaagagtt cctcatgggt tccactctcg ataatgcgtc ccttatccaa
     5821 gacatagatg cagttggcgt cttgaatagt agaaaggcgg tgagcgatag caatagttgt
     5881 tcggccctgt ctcatcttcg ccagagaagc ttgaaccaag ctttctgttt cagagtcaat
     5941 attggctgtc gcttcatcca aaatcaggat tttaggctgg ctggcgactg ttctagcaaa
     6001 ggcaagaagc tggcgctgcc cagtagagaa gctcgaacca cgctcggaaa caggggagtc
     6061 gtacccctga ggaagttctt gaataaagga atctgcatcc acaaaggctg ccgcagcctg
     6121 aacctgatca tcactgattt cttggtacat ggcgatattg gacttaatag ttccatgata
     6181 gaggaagggt tcctgcaaga ccaaaccgat gttttttctc agctcttctt gactgaaatc
     6241 cctgatatcc acatcatcca agagaactct ccctgactgg aattcataaa agcgcatgag
     6301 gacattgata atagacgatt tccctgaacc tgtatgacct acaaaggcaa tggtttcacc
     6361 cttattaaca gagaaagaaa tgtcatccag aatcggatgt ttaccgtcat atgagaaaca
     6421 cacatgttca aaacggatat tgccttcttg gactttggct tgcccatttt cttgaagagg
     6481 ttcataggtc ctctcgtcta tcagggcaaa gacacgacct gcagaaacca tagccgtttg
     6541 cagagttgaa aagttttgcg tcacctcaat caaggggtca aaaaggcggt tgatgtactg
     6601 gataaaggca tacatggtcc cgaccgttat cccgatagaa aaaccacggt agccaaagta
     6661 ggccatcaag actgcatagc ctagaagttt cagcaaactc atggcaggtc tcaaaaagag
     6721 ggcatccaag gctacagaac ggttggcgta gaccaagtgt tcttggttga tttcatcaaa
     6781 ttctgcctgc aggcgcttct cttgattaaa agcctgaata atcctgattc cctcgatatt
     6841 ctctgccagc ttactattga tatctgacaa gagacttctg gttttctcaa tgattttcac
     6901 tgattttttc cgatagagat tgaccaaaag gaaaatcaag gggagaaaga gcaagactaa
     6961 tgctgtcaaa cgaaaatcca ataccaacat ggtataaagg gttgtcagaa agatgaaaac
     7021 tgctgagata aagctggata aaatccccga aaacatatca ctgatagtct cggtgtcatt
     7081 tgtcaaacga gagacgatag agcctgctgg cgtcttgtca aagtaagaca tgcccagttt
     7141 ctccatattg gcaaaggcat cccgacgaat atccctaaca atactgtaag acacgcgcgc
     7201 aaagagaaga ttgcctacat actgaactac agtttgtagg atgtagagac catagtagac
     7261 cagcaaaacg gtaacggcta gttggttaag attgctgaga tactggtcga taaagtggga
     7321 agccacgagg ggtatgacac ttttaatgac cgtcgtcgct aggagaaaac tgagtgccaa
     7381 aaaggtcagg agtctataag gcttgagata agacatcaag cgcttcaata cagcccattg
     7441 ttcttgttta ttctgcatct tcttctcctt tcatttctaa ctgctgagac tggtaggttt
     7501 gggcatacca gccatccatc gctagcaagt cttcgtgcgt gcctcgttcg ataatttgac
     7561 cattttgtag aactaaaata aaatctgcat ggacaacagc actgaggcga tgggcagtga
     7621 taatggttgt cttgtccttt cgcgtctcct tgaggttgtc gataatcgca tactctgtct
     7681 tggcatctac ggcggataag gaatcatcca aaatcaagat atcagggtct aaaatcatag
     7741 cccgactcat agccaaccgt tgcttttgac caccagaaag actgactcct ttttcaccaa
     7801 tcagcgtatc aaatccttga ggcatgtcta caatatcttg gtaaactcgg gctagcttag
     7861 tagcttcctc gaccgctgaa aggggcaagt taggattgcc aaagcggata ttgtctagga
     7921 ttgaagttgc aaagagaaac tggtcctgag gaacatagcc catgagactg cgaaggtctg
     7981 tcagacgata gtcccgaata tcgtgaccgt ttagataaat ggcaccctta tccacatcgt
     8041 attcacgcaa gaggagcttg attaaggacg ttttcccaga gcctgtctgc ccaaccaagc
     8101 ccagtgtttg cccttttgcc aaactaaagt gaatatccgt cagtgtttcc tcattttcaa
     8161 aagcaaagct gtcaatggca tactccaaac gcccattttc aataccatcc agaggaaact
     8221 cagggtcttg tacaggagat tcctgagaca aaagattttc aatccgctgg taagaaacct
     8281 tccctcgctg agtagtatta aagaggaagc cgatggccat aagaggccag accagcatat
     8341 ccaaatagct gataaaggtg actagattcc caactgtaat ctgcccttcc tgaaccatca
     8401 aggagccaac caaaagcgtt aaaacatagg acgaaccaac aaacacgaga accatagggt
     8461 caaagagact atcatatttc atggtttgca ggttcttttg gaaggttaat tcattgactg
     8521 cctgaaaaga cttcaactcg tctgcctgat aaccgaaaga cttggtcact ttgatacctg
     8581 atacggactc ctgtaccttg ttattgagtt cagaaaaagc agcttgggat tcgccaaagg
     8641 ccttatgagt ctttctccct aggcgactag tcgcataggc catgaaaggt agggggagaa
     8701 tggcaacaag agtcatctgc catgagatgc taaagagcat ggtcaacaaa gtcaccagag
     8761 ccgtgataga ggcatccacc gcagacatga caccgccacc tgctaaacga gtcaaggcat
     8821 tgatatcatt ggttgcgtgt gccatcagat cacccgtccg ataggtttga taaaaggctg
     8881 atgacatttt tgtgaaatgc ttaaacaagc gagaccgcat gatctgtccc aagcaataag
     8941 aggtcccaag gatatacata cgccacacat agcgcaaata gtacatacca aaggctgcaa
     9001 gtagcaagta aaataggcta agaaggaggt cctgctgggt taattgcccc gatgtgatgg
     9061 catcaatgac ccgccccata accataggag gaatgagatt gagaacggaa accaagacca
     9121 gggccacaat cccgactaga taacggcgtt tttctaactt gaaaaaccac caaagttttt
     9181 gaataatgga cataaaatcc ctttctgatt gcaaatggaa acctgaggcc aatactcaat
     9241 gaaaatcaaa gagcaaacta ggaaactagc cgcaggctgc tcaaagcact gctttgaggt
     9301 tgtagataga actgacgaag tcagtaacct acatacggca aggcgacgtt gacgcggttt
     9361 gaagagattt tcgaagagta caagacccca ggtttttctt tttcataagt tacgactgta
     9421 acagcaccct tgtcatattc agcaataaag atattggcta cattgtcatg cccttgttta
     9481 ctgaggttat caagcaacca ctcttcgcta cgaccaatcg attccaagac atctacttga
     9541 atcacaccgt cagtcacaac tggatactta ggattttcat ctcccatttg cacaacgata
     9601 agttgcccat tttgctcttg cacagctcgt ttgacttgct tcatctggaa aatcccttgg
     9661 ctacgaagtt tgagggctac atccgatgca gacaaaccaa ctgaacgaca ggcttctggg
     9721 tcaatattcc catttttgat aaggagagtt ggcttaccat caatcaaacg tttcacaaag
     9781 tgaacattgt tgttgagcca tttgagagtc aagaccagtt tgattaaaat ttcgatataa
     9841 ttaagtgtca tttgttcaca tccaccagtt caattgcgtc tgtcttatac aattcgactt
     9901 tctctaacag gtacttgtct ggctcacttc cgtttagggc acgataatag cgatctccca
     9961 ccttgataag tgcgccatct gtgtttgtgg aagtattaac atagacttct gacttgtcta
    10021 ctcccaaatc tttggaaaca acctcgatga aatgaagtga agtttgaaat ttattgttag
    10081 aaacttgatt gttttgataa gtcgtgatgc tgaccaaaag cagagctaaa taaggccaga
    10141 gctgaaatca tgaccaactc acgaaattcg gttccctttt tatcatggta tgccttgaaa
    10201 gcaaaaaaaa ctgtcacagc taatagaaca atcccaaagc caaccatgat accattttgc
    10261 tgaccgattt ggctgagtac atagtcataa gagtagaatt tcatatattt tcactccctt
    10321 tcataaaatc attcttaatt ataaacgaaa gagagtaatt tttcaaacca tacgttgggg
    10381 aaatagatct ttttttggta taataaaatc tataatctga atgaaaaagg taactttatg
    10441 aaacttatct catggaatat tgattcccta aacgctgccc taactagtga ctcagctcgt
    10501 gccaaattgt cccaagaagt cctacaaacc ttggtcgctg aaaatgctga tatcattgct
    10561 atccaagaaa ccaagctttc tgccaaagga cctacaaaga aacacgtgga aattttagaa
    10621 gaactcttcc caggctacga aaacacatgg cgctcttccc aagagcctgc ccgtaaaggc
    10681 tatgctggaa ccatgttcct ttataagaaa gaacttacgc ctacgatcag ttttccagaa
    10741 atcggtgccc cttctaccat ggacttggaa ggccgtatca tcactctaga atttgatgca
    10801 tttttcgtaa cccaagttta cactccaaac gctggtgacg gtctcaaacg cttggaagaa
    10861 cgccaagtct gggatgttaa atatgctgag tatttggctg aactagacaa agaaaaacca
    10921 gtccttgcga ccggtgacta caacgtagcc cacaatgaaa tcgaccttgc aaatcctgct
    10981 agcaaccgcc gttcacctgg atttactgac gaggaacgtg ctggatttac caaccttttg
    11041 gcaactggat ttacagacac cttccgccat gttcatggcg atgttcctga acgctacact
    11101 tggtgggcac aacgcagcaa aacttctaaa atcaacaata caggctggag aatcgactac
    11161 tggctcacaa gtaaccgtat cgctgacaag gtcactaaat ctgatatgat tgactcaggt
    11221 gctcgccaag accatacacc gattgtcttg gaaattgact tgtaaggatt ttctatatgg
    11281 actacaatgc ggtcattccc gagtttcttg tatcgaatat cgaacagtcc cggtccttct
    11341 actgtggttt actgggtttt aggattgaat actaacgtcc agaagaaaac tttctctttc
    11401 tcttgaagag tgtcaactaa tgttagagga gggaacaaag gatcaactag ctgaactgac
    11461 ctaccccttt ggtcgtggag tcaatctctc ctttggcata aaggatgttc caaaactcta
    11521 tcaaaaagtg atggaggcaa actatcctat ttatcgtctc ttgaccaaaa gaaagtttcg
    11581 tgtcagtgat ccctatatct atcctcataa atttgcagtt ttggatccag atggctattt
    11641 tttaagattt agcgagtaga ataataaaaa ttgggaaaag tctttaaagt tagaaaaagg
    11701 catattgtca ggtgtctaaa aacttgatag tatgcctttt atgatggaaa aatcttctaa
    11761 tactcttcga aaatctcttc aagccacgtc aacgtcgcct taccgtaggt atatgatact
    11821 gactctgtca attctatcca caacctcaaa acagtgtttt gaggttgtgg atagcttcct
    11881 agtttgctct ttgattttca tttagtataa atgttagctt acaatgaagt ttttgttcac
    11941 cctctcttct ttttgttcgc tttttaaacc aaaacactaa caaaatagtt gatttctaaa
    12001 gttatttagt gtaaaataaa aacattggct caagcctatt taaaattgaa tatcgtttta
    12061 cttgtttatg tcgtgaattg gcacgacgtt tctacaaggt gccggaacac ctaacaataa
    12121 gtaagtcagc agtgagatat ggccgttttt gccatgtcta tttaggggac ttacttagag
    12181 attaagtagg tcttttttga atatttttaa tatttccctt aattagtgcg gacgggaggt
    12241 gaaattatcc tgtgaataat ttcaggaatc cagggaattt catccctaat ttttgagccc
    12301 ctagctcttt catttctagg cttaggctaa aatagttccc cgaactattt tactctcaaa
    12361 aatcccgtca tagacaataa caagattatt gtctatgata ccactacaac gggaaatatt
    12421 tgataagact cgcttcgctc gtaatagttt tatcaaatat atagtattag caaaagggat
    12481 cactatagaa atatttaaac aacaagtaaa ttaactttta gactttagga ggtcttatga
    12541 aattattaga agagcgcatc ctcaaggatg ggcatatctt gggtgataac atcctcaagg
    12601 tagattcctt tttaacccac caagttgact ttagcttgat gcgagagatt ggtaaggttt
    12661 ttgcggaaaa atttgctgct actggcatta ccaaggtcgt aaccattgaa gcgtcgggta
    12721 ttgccccagc cgtttttaca gctgaagcct taaacgttcc catgattttc gccaaaaaag
    12781 ctaagaacat caccatgaac gaaggcatct taactgctca agtctactcc tttaccaagc
    12841 aggtgaccag cactgtttct atcgctggaa aattcctctc accagaggac aaggttttga
    12901 ttatcgacga tttccttgct aatggccaag ctgctaaagg cttgattcaa atcatcgaac
    12961 aggccggtgc cacagtccaa gctatcggta tcgtgattga gaaatccttc caagatggcc
    13021 gtgatttgct tgaaaaagca ggctaccctg tcctatcact tgctcgcttg gatcgttttg
    13081 aaaatggtca ggtcgtattt aaggaggcag atctctaatg caaactcaag aaaaacactc
    13141 gcaagcagcc gttctgggct tgcagcactt actagccatg tactcaggat ctatcctggt
    13201 tcccatcatg attgcgacag cccttggcta ttcagctgag cagttgacct acctgatttc
    13261 tacagatatc ttcatgtgtg gggtggcaac cttcctccaa ctccaactca acaaatactt
    13321 tgggattgga ctcccagtcg ttcttggagt tgcattccag tcggtcgctc ccttgattat
    13381 gattgggcaa agccatggta gtggcgctat gtttggtgcc cttatcgcat ctgggattta
    13441 cgtggttctt gtttcaggca tcttctcaaa agtagccaat ctcttcccat ctatcgtaac
    13501 aggatctgtt attaccacga ttggtttaac cttgatccct gtcgctattg gaaatatggg
    13561 aaataacgtt ccagagccaa ctggtcaaag tctcttgctt gcagctatta ctgttctgat
    13621 tatcctcttg atcaacatct ttaccaaagg atttatcaag tctatctcta ttttgattgg
    13681 tctggttgtt ggaactgcca ttgctgctac tatgggcttg gtggacttct ctcctgttgc
    13741 ggtagctcca cttgtccatg tcccaactcc actctacttt gggatgccaa cctttgaaat
    13801 ctcatctatt gtcatgatgt gtatcatcgc aacggtgtct atggttgagt caactggtgt
    13861 ttatctagcc ttgtctgata tcacaaaaga tccaatcgac agcacgcgcc ttcgcaacgg
    13921 ttaccgcgca gaaggtttgg ccgtacttct cggaggaatc tttaacacct tcccttacac
    13981 cggattttca caaaacgttg gtttggttaa attgtcaggc atcaaaaaac gcctgccaat
    14041 ctactacgca gctggtttcc tggttctcct tggactgctt cctaagtttg gcgcccttgc
    14101 ccaaatcatt ccaagctccg tcctcggtgg tgccatgctg gtaatgtttg gttttgtatc
    14161 aattcaaggg atgcaaatcc tcgcccgtgt tgactttgct aacaatgaac acaacttcct
    14221 tatcgcagct gtttcaatcg ctgcaggtgt cggtctcaac aacagtaatc tctttgtcag
    14281 catgccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtagcca gcctactcgc
    14341 tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg tgcgagcctc
    14401 ttttttgttg acaaaataaa aacatctacc tattcatata tgctagtgtc atagtcctga
    14461 aaatcatctg catcaagaac aatttcacaa ctcttatact tttctcttac aagtcgttcg
    14521 gcttcatctg gattttcagc ctctatactt actaaacgtg ataaagtttc tgtaatttct
    14581 actgtatact ttttcagttt tagattgctt tccaataatt cacctattaa caataatcta
    14641 tgataggagc tatgtagcca ctctggcaat ttatctccta cttcccaccc atatttttcc
    14701 ttcatcaaag ctagttgttt ttcgtcacca aatttcatat tttcagataa ccatctcaca
    14761 caatcgggat ctgataaggt acagacgatt aaacttaggt tatcgtaacg gctttcagtt
    14821 tcataatcag gccagatgac ttttaagaaa tcatacacct cttctgatgc aacaagttgt
    14881 ttcgttttca taatttccga tactttcctc tacctttaaa ttctattatt tctttgtctc
    14941 ttaatatttg aagctgttgc ctaatctttt ctttgatatg attgttctta acaaagatat
    15001 tttttaggtc actttcaaaa cgatacatat cttcaagggt aaattctgaa ccctctatct
    15061 tatctataca atttagaatt tctattgtcc aacctcttga tgacagagag ctcttcctta
    15121 aaaataaacc ttgcttaaat tcttttgtaa ctttttctgg atctctaact tgtccatctt
    15181 gcacaagaaa tatccttcct ttagaaggta cttgtgataa atcaatgtta caaccaatcc
    15241 aacctgctcg tctagcagtt ggtgcaagtg gttttctttg aataatcgat ttcggtgtaa
    15301 caaattgctt cggaaggaca agaaagttat ttacctcaaa attttttgtg taagttaaaa
    15361 aaaagaaatt aggattatta tctgcctgca cacgcttcat catcgttgca taagcaccat
    15421 cattgattgt tgatgaaaaa tttccttttt tgctctttag ttcaaactcc tcactacaat
    15481 gattacagta aaaatctgct acaggccgat tattttcaaa atgatttaag ggattgttcc
    15541 cacaattagg gtaataagac tgacgataaa cccaatcctc cgttaagata cgtgcctttt
    15601 ggctattact tttataggtt tctactaatt ctaaattaaa gtgtaattcc atttaaaaat
    15661 tcctcactaa tatgagaact cactcgatat aagcaattaa aatctactat aactagtata
    15721 ccacatatat gtacctatta catttgtatt tcatcactct cttttatatt tccttctaac
    15781 atatctcata aattaaaaat gagtgagata aatttatccc actcaccttt ctttatatcc
    15841 ttacccgtct ttcctcagac ctttggccta tgacaaacat ggtaaaggtt gctttgcaga
    15901 catttctgcc ctcttgattg gtgatatcca catccatcac acaggttgta cggccttgat
    15961 ggacacattc tcctttaatg gtcaatacat cgtcgagttt tcctgcctta aggtagttga
    16021 tagaggattg gagtgtcacc ccatcaagtc ccagcgagat aaccaccaaa ccactgattt
    16081 ggtcacatag cgtgaataga tagccaccat gggcattgcc atagtagttg agcgacgagt
    16141 tcactacttt ggtcgtcacc acaacgtgac catctctcat tttttcaatt tcgtaatttt
    16201 caaaggcaga tatagcgtca aaatgaaagt ctttcatcgt ttcctcctga tatttcaaac
    16261 gacactatga tactactttt tataggactg tgcaaggaga aagctcctag tctagtaaaa
    16321 ttccgacatg ttccacaaag cgcataaagg ctgtctgccc ttgttctgtc tgcttgtaga
    16381 ctcctgcatc ctcaagtaca cgcgcaaaga tagcacccac agagtccttg acgattgcaa
    16441 gggctttttc tttatccgtt aggtctggat gttgggattt gagttggtct gcccactcct
    16501 gatgataatc ggcaactgta acagcttctc ctacaagata gctcgcgact tgctccactt
    16561 cttctttcag acgtggtggc aagattgcca agcccatgac ctcaatcaag ccgatatttt
    16621 ccttcttgat atgttggaca tccttgtggg gatgatagat accatcagga tactctgctg
    16681 aagtctgatt gtctcgcaag accaagtcca actcaaactg tccatcgcgt ttgcgggcaa
    16741 tgggtgtgat agtgtgatgc ggtgtcctgt ctgtctctgc caaaatctgc actgcaggat
    16801 ctgaatactg gcgccattcc tgcaaaatct tatcagccaa attgatcaaa tcctctttgg
    16861 aatccgaagt caaacgtagg acagacatgg gccacttgac aattccagcc ttgacctgct
    16921 caaaaccagc aaatcggaag gccttttgca agggagccaa ttccatagga aatacgtgac
    16981 ggcctccctg ataatgatca tgagttagaa tagagccccc cacaatcggc aggtcggcat
    17041 tagatccagc aaaatatcct ggaaactggt ctacgatagc caacagacgt tcaaaactct
    17101 gacgactaat ggccatggga cgatgctggc catctaaaaa gatacaatgc tcattaaagt
    17161 aagcataggg cgaatactgg aaaccccatt cctgaccaac catttcaaaa cggataatac
    17221 ggtgattgct acgagctggg tggttaactc gaccatggta gccctcattc tctagacaaa
    17281 gctgacactg aggataatta ctagcttgca ccaacttggc tgccacaatc tctttcggat
    17341 ccttttcagg cttagagaga ttgatggtaa tttcaagttc tccataatca gatggaacac
    17401 gataagcgat atttttagca atggccttga gtttgatgta gtcatttttc tgactgagtt
    17461 gataaaaatc ctctatcgct tgctcaggat agtggacata ggtttcccag aagtcacgat
    17521 tgacctgact cggacaagga gtcaccaagt ccatcagttc agtaccaagg atttcacgcg
    17581 cagtctgact atcctcaatc gtctctaatc gaacggcttc ctcaactagc tggtccttga
    17641 ggtcaatcaa tttatccaga ttggtctcaa cttccaaaac accttctccc actcgtgcca
    17701 aaacacgatt ggtcaggtag attcgatcca tttcctcaaa tgaactttca gaaatgacat
    17761 gtgttacaaa tttatctact aaggtcacca tagagcctcc ttttgactag tcaaggacgt
    17821 gagtgccacc tgcaacttca gcgatataga agcttggagc gtatccaact acttcctcgt
    17881 agtgtttgcc tacagtttcc ttaaaggcct caacagtatc tttttgaacc aaggcaatgg
    17941 cacagccacc aaaaccagcc cctgtcatac gagcaccgag aactccttct tgtgcccaag
    18001 ctgtgtgaac aagagtatcc aattccaaac cagttacttc ataatcatgc tccagagaaa
    18061 cgtgtgacgc attcatcaaa cgaccaaatg tttccaaatc acctgcttga agggctgctt
    18121 gagctttaag ggtacgttgg ttttcaagca cagcatggcg agcacgtttc aaacgatttt
    18181 catctttaat caaatagcta tattggtcaa cggcccactc gtccaattca cccagggtct
    18241 gaatatccaa ggcaacttgc aattcttcca ctgctttttc acactcagca cgacgttcat
    18301 tgtatttaga gtccgccaat tcacggcgtt tgttggtgtt catgataaca acgacattgt
    18361 ccttcaaatc aagtggcacc aaatcatact ctaaggtgtt ggtatcaagg taaatagcac
    18421 gttgatcggc ccccatacca atagcaaact ggtccatgat accagagttg actccgataa
    18481 agttgttttc tgtttgtttt ccgattttaa ccaaatcgag acgctctaat tttaaatcaa
    18541 agagatgctc tgccacgact cctgtcaaga gctccaagga tgctgaagaa gacaagccag
    18601 caccatttgg gatattccca taaacataaa aatcaaaacc tttgtcaatc acgtgcccag
    18661 cttcttgcaa gaaatgaagg acaccttttg gataattggt ccagttgtgc tctttttcaa
    18721 acttgaggtc agcgagaggc acttcgataa tgcccttgtc ctcaaagtta gctgagtaga
    18781 aacgcaagac ttggtcgtca cgcttacgag ctgcaccgta agttcccaag gaaatagcag
    18841 caggaaaaac gtgcccaccg ttgtagtctg tgtgttcacc aatcaaatta atacggcctg
    18901 gtgaaaagaa ggtttggtct gcttcttgac caaaaacagc aagaaagtct ttgcgaagga
    18961 cttcagtagt aagatgttgt gccatatgaa ttctcctttg actgtccgtt aagccatctt
    19021 aacgtgtaat cgttttcttt ctattgtatc caaggaattt cccaaggtca atccttttta
    19081 ctaaaatttt actaaaatat cagtaaaaag aagaaaaata tgatatacta acctaatcaa
    19141 ggaggattta tggctacctt aaaagacatt gcacagctag cctctgtctc aatcgcgacc
    19201 gtatcccgtg tcctcaaccg cgaccagagc ctatctgtta cagaagaaac cagacaccgt
    19261 attttaaccg ttgctgaaga gctaggctac accaaacacc tcaagacagg cgagtcccac
    19321 aagcccaagc aaaagattgc cattatccaa tgggtcagcg aacaagggga gctagacgac
    19381 ctctactact accagattcg cctaggaata gaaaaaagag cccaagagtt ggactatgat
    19441 atcttgcgct attttaatga ccatcctttt accctaagcg aggaagtgat tgggattctc
    19501 tgcatcggaa aatttagccg agctcagatt tctgcctttg aagaatacca aaagcccctg
    19561 gtctttctag acagtgatac actttccctg gggcatactt gtattattac ggatttttac
    19621 actgctatga aacaggttgt cgattatttc ctcagtcaag ggatggaccg catcgggatt
    19681 ctaacagggc ttgaggaaac aacagaccaa gaagaaatca ttcaggacaa gcgtctagaa
    19741 aacttcaaaa actacagtca agcgagggga atctatcatg atgaactggt ctttcaagga
    19801 agatttactg cccagtctgg ctatgactta atgaaggagg ccattcagag cttgggagac
    19861 caacttccgc cagcattttt cgcagccagc gatagtttag ctatcggtgc cctccgtgcc
    19921 ctccaagaag ctggaatcag cctgccagat cgcgtcagcc tcatttcatt caacgacacc
    19981 agtctgacca aacaggtcta tcctcccctc tctagtatta cagtttatac tgaagaaatg
    20041 gggcgagcag gtatggatat tcttaacaag gaagtcctcc acggtcgcaa aattcctagc
    20101 ctgaccatgc tgggaaccag actgacatta agagaaagta ccctaaatca agaataggat
    20161 aacataaaaa acgaatagag ttctaaaact cctattcgtt ttttattcga ttacaatcat
    20221 agacttaatt gtcttacgtt catccatatc tttataggct tggtcgatat cttccagttt
    20281 ataacttgaa gtaaagacgc gacctggatt gatatcacca tcaaggacgg cttttagtaa
    20341 aaattgctta tcgtatgttg tagcagaagc tgccccacct gctacagaga tattttgcat
    20401 aaatgtcgaa ccaagagcac gattattata gtgtgggact cctacaaagc ccatacgccc
    20461 tccattatga agaacaccta gcgcctgttc tatagcagcc tccgtaccaa cacattcaag
    20521 tgctgcgtct gctcctccgc cgaggatttc acgcaccttg gtaattcctt cttgaccacg
    20581 ttctgcaaca acagctgtcg cacctgactc catagccatc ttttgacggt cttcatgacg
    20641 gctcataagg ataatttgtg atgctccacg catcttagcc gcgatgacag cacattgacc
    20701 aacagcccca tcaccgataa caacaacctt gtcccctttt tgaacatttg caacacgcgc
    20761 tgcatgatag cctgtcggca tgacatctgc aagagtcaaa agggacttga gcatcccttc
    20821 tgtataatca gaaggttgac cagggatttt aaccagcgcc cagtttgcat agtggaagcg
    20881 aatatattct gcctgaaaat caccccccaa attattacca atatgattgt cgcaagaacc
    20941 gtcaaatcca gcaagacagg catcacactc accacatcca tgtgtaaaag ggacaatcac
    21001 aaaatcacct ggtttcaccg tcgtaatggc ttccccagct tcttcaacaa tcccaatcgc
    21061 ttcgtgtcca ctatttttat gtccagcttt cgtttctgga ttacggtacc tccataaatc
    21121 tgaaccacaa acgcacgcac gaaccacacg aataatcaca tcatccgctt ctattatttg
    21181 cggacgttca atgctagcaa gtccaacctg acctgccttt gtatatactg ctgatttcat
    21241 ttaaaatttt ccttccttat aaagtttaat tttgagattt aaacgattta aagctgtctg
    21301 tagctgagac aagcgctcct ctaatttttg cttttcctct tctaaaatac caagcctctc
    21361 ctctctcgtt tcatctccct tttggtagag cgacatatag tcaactaaac tatctacaga
    21421 gacacccgcc gaacgaaaac acttaataaa ttccagcgct tcgatatcct gatcttgaaa
    21481 atcacgaatc ccagtagcag tacgagtaat cggtggcaca agaccaaccc gttcataata
    21541 ccgaatcgta tccgctgaaa ttcccaacaa atcactggca gatttaatat tcacacgcta
    21601 acctcctctc atttcattca ctgaattcac gctattctaa ctcacttatc ttattatata
    21661 actttgagtt gactccaagt caagcatttt gtatctaatc gaaaaatttt tatcattaac
    21721 aatcatatat ctggagttta tacaatgctc ttctcttacc aatttttgaa atatttaaat
    21781 aataaagaaa ttactctatt ccaaataaga tagtacccaa gtatttttca cttttttcta
    21841 atgttgatgc tcataactcc gttccaagtc acacaccttt cgcttatggg tttggtgagt
    21901 ttctaggtca gcatcaattt caatggtaat attttgaaaa ccacaatctt ttaggaaaat
    21961 tcgaatagac tctttacaag tttccatatg ttccatttct tttagacaaa catgtacaat
    22021 ggcatttttt tccaaagcat ccatagtcca gagattaagc tgattaaggc tggccacatt
    22081 gtccaatcgc tccaggccac tctttacttg cttgatatca agaccttctg gcacagcatc
    22141 caagaaaatc ttgagtgtag accaaaaacg tggaagggct tttgaaagaa taaagaaaga
    22201 aatgacaagg gacaaaagag gatctaggat ataccagtcc gtaaatcgaa gaacaatcgc
    22261 catcaggata acagctaccc accctagcgt atcttccaga aaatgcaaac tcagaataga
    22321 ctcattcttt gtctttccct taccaaccac cagactcgct aacagattga tagtaatcgc
    22381 aataattcct aaccagagaa tcccctcatc attgactggt tgcggatgca aaatcttcgt
    22441 gacattttcc aaaatgacta gaacagagcc cgttacgaga atcacagctg ttaccaaggc
    22501 tcctagcagg ctaaaccgct tatagcccaa ggtgtactga ttgtcttctt cacgattgga
    22561 gattgtttct agaaaagctg atattccaat tgcaatcgca tctcccaagt catgcacaga
    22621 gtcagcaaga acagcgctag aaccaaatac tccacctgca ataaactcaa caatggcata
    22681 agtcaaattt aagaaaaaag ccacccaaac agcatatttt gccttcatat ttctcattcc
    22741 tttgttataa tagatttatg aacaccttgt tcattatcat tatccactaa aactcaaaga
    22801 aaggatagaa gcaaaacgtc agctttattc agttctgaac aatttgcctt aagtgtccat
    22861 atgactaaca ttgaccgccg tatcagcaaa accaaaaaag ccatctatca agcttttata
    22921 caacttttga atgctaaggg ctacgaggcc actactgttc aggatatcat tgatctcgca
    22981 gatgtgggaa gatccacctt ttactgtcac tatgaaagta aggagcttct tctggaccag
    23041 ctctgccgct acctcttcca tcatcttttt gaaagagagc aagccatttc aaccgaggac
    23101 tacctcgccc acctctttct ccattttcag aaaaaccaag accatatcac cagtctgcta
    23161 ttttccaaaa atgactactt cctccgtcaa ctccataaag agctagaaca ccatgtctat
    23221 tccgtgctag ctgataattt aaaagaagcc caccctaata tgcctacttc ttacctccaa
    23281 cacttggtcg tgtccaactt tatcgagaca ttgacctggt ggctcaaaaa aggtcaagat
    23341 ttcataggcc aggaagttgt ccaattttat ctagaccttc tcattcctaa aaattgaata
    23401 tagagtaaag ctcagttgtc ttatttctag gttactgagt tttttatctt ttcaacaaca
    23461 aaagaggacc cgccgatcct ctttttcata ctataaatcc ttgattatca actatatctg
    23521 ttttaatcga aatctcaaaa cagcactttc aaacatcttt tcctagttaa gtaaatcagt
    23581 attctgctta gctgccttgc tccattgata ccaaccaact agactgttaa tgagataaat
    23641 tagatatttc ccttgaattt gcaggctttc tccccaccag agatagattg aaaaaacatt
    23701 ggtagccgcc cagaatatcc actgttcacg gtaaacagct gtcatgagga tttgccctac
    23761 cccattggtt gcatctgtga ttgaatcacg atagggacga ttggcaccaa tagactgata
    23821 aatgaagcca aaggccaacc accaaagcac actaatggaa agatactttg tccagccctt
    23881 gccgtccagt ttacgcgcga caaactcctg cttttccttc ttaaactgtg cctgataaat
    23941 ccaaactaga agtccaattg gctgcatgac tgtgaagtaa agtgtcgtca gcacctcacc
    24001 ataaaagcct ttctgtaggg ccaaaataag gtaaataaca gagttaatca agccaaaaag
    24061 ataattactt gctcgacctt ccgatacaaa gattacacag ataatccctg tcaagctaca
    24121 aatcatccca atccagtcaa caatacgatg ttcgtaaacc aactccagcc agagaggaaa
    24181 acttcctaaa accagcaaat aaatccactg ggcaaaacta cgatgggcaa agaggtcatc
    24241 ccagatagcc ttcatagttc ctgaaaatcc taaatcagcc atagccgcaa ccatacgacg
    24301 gtaaccacct gacatttcac ctagggttgt tttgatattt tcaattttct tttgcaaata
    24361 agtatgcatc atttctcctt ttgtttttaa agagccgtgt ctggatagac tttcggacgc
    24421 aacgctctat tagataatga actgcctata cacaagattt ctaaccttag tcgacatgag
    24481 ctaaaacctc ttatttgtta agtagttcac aaaatattat acacctattt tatgaatagt
    24541 caactgtctt tacagtaaaa ttttagaaaa tcatgaaaat tttctctttc tttccatttt
    24601 aagtgacatt cagtcattct cacatcaaaa aagcccagac gaaattgtct gagcattctt
    24661 ttatctagtc gtttaaggaa gttgagttca gtatgtttaa agtctctgtc ccatcatttc
    24721 ttcaacaaac cttgttcttg gagaaactcc ttggctactt gctttgctga cttgccttca
    24781 acaccgactt ggtagttgag ctggctcatc tggctttctg taatcttacc agccaatgta
    24841 ttaagaactc tttccaactc tgggtgtttc ttgagaagag cttctttcat gagtggagcc
    24901 ccttgataag gtgggaagag ttgcttgtca tcttccaaga cctgtaaatc ataacgctcc
    24961 aattccgcat cagtcgaata ggcatccgtg atttgaatat cccctgactg aattgcctga
    25021 taacgaagag ctggctgcat agtcgccaca ttgagattga gaccatacat tgattgcaag
    25081 cccttatttc catcttcacg gtcgttaaac tcaagcgtaa agcctgcctt cagctgccct
    25141 tccacttttt tcaaatccga aatggtcttc aagccatatt cttgcgcaat ctttttcgga
    25201 acagctacag cataggtatt ttgataagac ataggtttga gataggctag atgatcctgc
    25261 ttggcaatgc tatcacgcgc cacttgataa acctgctctg gctcatgact caccttgggt
    25321 gatggttgaa gcaaactttc agtcaccgta ccggtaaatt caggataaat gtcaatatca
    25381 ccttttttca gagcttcata aaggaaactt gtcgtcccaa aattcggttt aacagtcgca
    25441 gtcatgctgg tattttcttc aatcagcaat ttatacatat tagccaaaat ttctggttct
    25501 gggcctattt tcccagcaat aatcaagttt tccttctctt tttgagccaa aagggctgga
    25561 ctataagaca gacccagtaa taaagccacc aaggcaaaac cagagaaaat cgtccgcaat
    25621 ttcgcttttt ccattacttt tagtaggaag ttaaaggcaa tagctagcac tgcagaagaa
    25681 agggccccaa tcaaaatcaa actggcatta ttacggtcaa ttcccaacag gataaaggaa
    25741 cccagtcccc ctgccccaat caaggtcgcc aaggtagccg taccgataat caagaccgct
    25801 gccgtccgaa ttccagacat gataacaggc atggcaagtg gaatttcaaa cttcttgaga
    25861 cgctcccatc tggtcatccc aaaggcaatc ccagcctctt gaagactcgg atcaattccc
    25921 ttcagcccag taatagtatt ttgtaaaatc ggaaaaatcg cataaatcac tagagctgtc
    25981 aatgccggta aagtcccaat tcccatcaag ggaataaaga gtcccaacaa ggccagagac
    26041 gggatggtct ggaaaatccc tgcaatctgt aagacccaat ccgccaactt ctcatgatag
    26101 cgaagaaaaa cagccaaggg aatcgcaagc aaaatagcta gtaacaaggt caaaagtgac
    26161 aactgcaaat gttgagatag agctgtcaac caatcactaa aacgatcctg aaaagttgca
    26221 attaaattag tcatgaacac tacctccaaa caagtctgct acaaagtctg ttgcaggcgc
    26281 ttttaaaatt gtctcgggat tcgctacctg gcgaatttct ccatcctgca agacagcaat
    26341 acggtccgcc aacttcaagg cttcatccgt atcatgggtt acaaaaatcg ttgtcatccc
    26401 aaactcttta tgcaattctt ttgtcagaac ctgcaactgt tttctcgaaa tagcatccaa
    26461 ggccgaaaag ggttcatcca tgaggaaaat cttgggctga ccaatcatag ctcggacaat
    26521 accgacccgt tgctgttctc caccagataa ttcactaggt aagcgatgcc catactcggc
    26581 tactggtaaa ccaaccttag ccaaaagctc ttctgttttc ttcgtaattt cttccttgct
    26641 ccaccccttc atttcaggaa tgagagcaat attttccgca actgttagat ttggaaaaag
    26701 agcaatagcc tgtaaaacat aaccagtaga aagacgaagt tcacgctcat catagtcttt
    26761 gatgcgcttc ccatccatat aaatatttcc atcagttggt tccaaaagac ggttaatcat
    26821 cttgagcatg gtcgtcttac ctgacccaga aggccctact aaaaccataa attccccatc
    26881 ctcaatctgt aagttgacat ctctcaagac atccttttct gtgtagcata gtgctacatt
    26941 tttgtattca atcattcttt gtcctcattt aaaacttccc tcgattggtc aagtcttcta
    27001 ccttaggcat gacttcctta ttatcccaat gctccacaat tttcccgttc tttaaacgga
    27061 agatatcgta ctgggcataa gcaacgccat caatctgagt ctgaccatag ctaaccacat
    27121 agtttccttg tcctaagagt tggaaaacaa agtcaaaagt gacactatat tcagccacat
    27181 agtttttata agcagcactt ccttgtccaa tatcatgatt atgctgaatc aaatcgtctg
    27241 ccacataatc actccactgc tctagctccc cattttggaa aatttctgtc aagaaacggc
    27301 gaaccagctt tttattttct gctttcttgt ccaaatcctt gatttcaaaa tctccaaaaa
    27361 tttgatctag ttggtcattt tcaggtgttc gatagtagtc aatgacatcc caatgctcaa
    27421 caatacgacc attctcatcc gcacgaaaag tatccgtcgt cacccattga gcttctccac
    27481 cattcagata ttgatgaaca tgaacaaaga ccagattgcc atcctcaatg gtgcggacaa
    27541 tcttaatctg acgctctgga tgacgctcaa agaaatctgc aaagaaggct gcaaatcctt
    27601 ctttcccgtc aggaacacct gtcgaatgtt ggatataggt agcccctaca gactgggctt
    27661 gagcctcagc aactcgtccg tcttgaatgg catggatgta taggttgtga gcatttttca
    27721 cttgttgtga catattctaa acctcatttc ccttctcttt cagattcgcc aaaattcttt
    27781 cttgaaaacc ttcaaattgg tgaatttctt cctctgaaaa tcctttgtaa aagatagtat
    27841 ccaatttctg actgacacga tgccccactt ctttctggga cttgcctaac tccgttaaaa
    27901 ctaaatactt cttacgcttg tcttttccac acggactaac aattacaagc ttttgttcct
    27961 ctagcttttt tatcatagtc gtcagcgtat tattcgcaag tccagtcgca agcgcgatat
    28021 ctgtcgcagt tgcgcagcca gtttcactat tccataaaac cgctaaaatc ttgccctgtt
    28081 cacccctata aagagcctca ggatcttgac tcagtaactt ttgaaaaatc cgcccattca
    28141 acaaacgaat atgatgggct agcaaatgac catctttcat aacacctcca atttatttcg
    28201 atatcgaaat gaataaaaca attgtaacac tcatcgttct aactgtcaac tatttcgatt
    28261 tagaaataat ttttgataat tatccacacc accatactcc ggctcaacta acttttaacg
    28321 agagtttcta aactccttcg tcctccagtc tacaaaagcc ttccatccgt actatcctat
    28381 attttatgag gggacacatt tttcctatca gaccatttat tttaaagata gaagtaaatc
    28441 ataattgctt ccatctgttc ttttatagta tattgaagtt ggactagggc actgtatctt
    28501 ctaaaacatt gatagaaagc gatttgaatt tcccaatcaa tttgttcgtc tttatagcat
    28561 ttcgaaactg gaataggaca ccatgactgc taaaagattt ctataaattc atttgatttc
    28621 ctcaatcaat ttgttcatat cttatttcat tccgctataa tttcacctta ccctatcttt
    28681 ttcgtagcac ccttcaaaca gcctatcccc taccgtttga cgattcctca cttcactcca
    28741 cttccattac agaagtttct tcactactat ggtctcggct gacttctcat gattccttgt
    28801 tactactatt tgaacgctca cgagataggt cttataaaaa atgctttgat ccacaatgga
    28861 atcaaagcat tttaaaaagt tcctcataca taagtgcaga agtcgcagtt cctctgtact
    28921 tggcttcttc tcttttgata aagcgagcca agttgagcaa ctcaggtgct ggatgtttgg
    28981 gatttaggag caattcacga ttgaccaggc ctgagagacg aactgcctgc aattgctcat
    29041 ttgtagtagg cagtttttta gtagtctcta ggagagcagc aactaaatct tcactcaaat
    29101 catgtcgagc atgattgtaa agatctttta taaggctttc taggtttggt tctgccatcc
    29161 ctaccacctc ccttatggtt taataatgtt taatcaaatc aaccgttgaa cgatccaatt
    29221 tcttcaccaa ggcttgtaag aaagcttgcg cttctaggaa gtcatccatt gcatagaggg
    29281 tttggtgaga atggatataa cgagcgcaga caccgatagt tgttgatggg acaccaccat
    29341 ttttcagatg agctgcacct gcatctgttc cgcctttacc acagtagtat tggtacttga
    29401 taccagcttc ttcagccgtt gtcaaaagga aatccttcat ccctgggaga agcaagtgac
    29461 ctggatcata gaaacgaatc aaggttccat ctccaatctt gccttgacca ccgtagacat
    29521 cacctgctgg tgagcaatca actgcgagga agacttctgg gtcaaacttg gttgtagagg
    29581 tatgagcgcc acgcagacca acttcttctt ggacgttaga acccagatag agttcattgc
    29641 cgagtttttg acctaataaa gcttcagcta gctcgcttac catgaggaca ccgtagcggt
    29701 tatcccaagc ttttgagatg atattttttt cattggctgt caaaattgca gaactatctg
    29761 gtacgatggt atcaccagga cggatgccaa aactttctgc ctcagccttg tccgcaaaac
    29821 caccatcaaa aacgatatcg gcaatggctg gcatggttgg tccccccttt ccacgagtca
    29881 aatgcggagg aacagaacct gaaatcacag gaatttcatg accatcacga gtcaagagtt
    29941 tgaaacgttg gctgctaacc accatggggt tccagccacc gatttctacg acacggaagg
    30001 taccatctgg cttgatttcg ctgaccataa aaccaacttc gtccatatga gaagcgacca
    30061 agacgcgcgg tgcatccaca gcttctgaat gtttgatacc aaaaatacca cccaagccat
    30121 ctgtcaccac ttcatccaca tgcggtgtca acttttcacg aagataagca cggacaggcg
    30181 cttcatgacc tgagactgca gcaagttctg ttacttcttt aatttttgaa aataatgttg
    30241 tcatttcagt tccttctttc tttcatccat tttaccactt tttataggag aaggatagtg
    30301 ggaaggtgga tttctaagtt agtatcttag tcctgctcta tcttagaaaa ggatagtatt
    30361 ctcttgcatg tagtgcaaaa tctagtaaac attccaaaat taactcgaat atttatttcc
    30421 aaacaaaaaa acaatacacc atcaaagttg tttggatttt tcatgaaatt tacagaaaat
    30481 agttgacttc cctttcttct ttctttaaat
//
LOCUS       DAVKQB010000027        29891 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKQB010000027 DAVKQB010000000
VERSION     DAVKQB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.027"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            108..1112
                     /locus_tag="VW528_001187"
     CDS             108..1112
                     /locus_tag="VW528_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9912010.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPEFVLKEAGQVIE
                     "
     gene            1181..2047
                     /locus_tag="VW528_001188"
     CDS             1181..2047
                     /locus_tag="VW528_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9912011.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGHMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            2044..2802
                     /locus_tag="VW528_001189"
     CDS             2044..2802
                     /locus_tag="VW528_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912012.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRVIQDLKQEEKAKMKISDYYQLFE"
     gene            2892..4652
                     /gene="dnaG"
                     /locus_tag="VW528_001190"
     CDS             2892..4652
                     /gene="dnaG"
                     /locus_tag="VW528_001190"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9912013.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPYQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRVGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VKEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            4655..5764
                     /gene="rpoD"
                     /locus_tag="VW528_001191"
     CDS             4655..5764
                     /gene="rpoD"
                     /locus_tag="VW528_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9912014.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            5778..6107
                     /locus_tag="VW528_001192"
     CDS             5778..6107
                     /locus_tag="VW528_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9912015.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            6222..7265
                     /gene="cpoA"
                     /locus_tag="VW528_001193"
     CDS             6222..7265
                     /gene="cpoA"
                     /locus_tag="VW528_001193"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9912016.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            7281..8606
                     /locus_tag="VW528_001194"
     CDS             7281..8606
                     /locus_tag="VW528_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9912017.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            8637..8813
                     /locus_tag="VW528_001195"
     CDS             8637..8813
                     /locus_tag="VW528_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9912018.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIVPVATDY"
     gene            8872..9006
                     /locus_tag="VW528_001196"
     CDS             8872..9006
                     /locus_tag="VW528_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9912019.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            9070..10380
                     /gene="obgE"
                     /locus_tag="VW528_001197"
     CDS             9070..10380
                     /gene="obgE"
                     /locus_tag="VW528_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9912020.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            10390..10548
                     /locus_tag="VW528_001198"
     CDS             10390..10548
                     /locus_tag="VW528_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912021.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(10600..11926)
                     /locus_tag="VW528_001199"
                     /pseudo
     CDS             complement(10600..11926)
                     /locus_tag="VW528_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13029..14288
                     /locus_tag="VW528_001200"
     CDS             13029..14288
                     /locus_tag="VW528_001200"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9912022.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            14304..14855
                     /locus_tag="VW528_001201"
     CDS             14304..14855
                     /locus_tag="VW528_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9912023.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            14848..16125
                     /locus_tag="VW528_001202"
     CDS             14848..16125
                     /locus_tag="VW528_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9912024.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            16141..17001
                     /locus_tag="VW528_001203"
     CDS             16141..17001
                     /locus_tag="VW528_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9912025.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVIGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            17224..17334
                     /locus_tag="VW528_001204"
                     /pseudo
     CDS             17224..17334
                     /locus_tag="VW528_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
     gene            17380..18184
                     /locus_tag="VW528_001205"
     CDS             join(17380..17748,17750..18184)
                     /locus_tag="VW528_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9912026.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLLRRSQWVEVTLVQSGFTISR
                     TPLSSEDTVMIDATEVKINRPKKELANDSGKKKCHAMKAQAIVTSQGRIVSLDIAVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTVEDKAYNHA
                     LSKDRSKVENIFAKVKTFKMISTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            18376..20667
                     /gene="pcrA"
                     /locus_tag="VW528_001206"
     CDS             18376..20667
                     /gene="pcrA"
                     /locus_tag="VW528_001206"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9912027.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            20710..21390
                     /gene="radC"
                     /locus_tag="VW528_001207"
     CDS             20710..21390
                     /gene="radC"
                     /locus_tag="VW528_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9912028.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(21387..22076)
                     /locus_tag="VW528_001208"
     CDS             complement(21387..22076)
                     /locus_tag="VW528_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9912029.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDSGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELTLIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(22094..22735)
                     /locus_tag="VW528_001209"
     CDS             complement(22094..22735)
                     /locus_tag="VW528_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9912030.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(22818..23033)
                     /locus_tag="VW528_001210"
     CDS             complement(22818..23033)
                     /locus_tag="VW528_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9912031.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(23041..23388)
                     /locus_tag="VW528_001211"
     CDS             complement(23041..23388)
                     /locus_tag="VW528_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9912032.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(23393..24508)
                     /locus_tag="VW528_001212"
     CDS             complement(23393..24508)
                     /locus_tag="VW528_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9912033.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(24518..25477)
                     /locus_tag="VW528_001213"
     CDS             complement(24518..25477)
                     /locus_tag="VW528_001213"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9912034.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGAPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(25590..26159)
                     /locus_tag="VW528_001214"
     CDS             complement(25590..26159)
                     /locus_tag="VW528_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9912035.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            26287..26958
                     /locus_tag="VW528_001215"
     CDS             26287..26958
                     /locus_tag="VW528_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9912036.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            26942..27760
                     /locus_tag="VW528_001216"
     CDS             26942..27760
                     /locus_tag="VW528_001216"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9912037.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            27757..28653
                     /locus_tag="VW528_001217"
     CDS             27757..28653
                     /locus_tag="VW528_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9912038.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHLLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            28697..29671
                     /gene="pta"
                     /locus_tag="VW528_001218"
     CDS             28697..29671
                     /gene="pta"
                     /locus_tag="VW528_001218"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9912039.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         8947 a         5353 c         6702 g         8889 t
ORIGIN      
        1 tttttgtgtg aggatttttt tgatggaggt tagtatagaa agaaaacgat ggcgtcgctt
       61 gtttagataa gtgaaatatg ttaaaggaaa taaaaaggag aaacagaatg aaaaataaac
      121 gtttaattgg aattattgct gcattagcag tcttagtagc aggaagcttg atttattctt
      181 caatgaataa atcagaagct cagaataata aggatgagaa gaaaataacc aagattggtg
      241 tgcttcaatt tgtgagccat ccatcccttg atttgattta taaagggatc caagatggac
      301 ttgcagaaga aggatataaa gatgatcaag ttaaaattga ttttatgaac tcagaaggtg
      361 accaaagtaa ggttgcgaca atgagtaaac aattggttgc aaatgggaat gaccttgtgg
      421 ttggtatcgc aacaccagca gcccaagggt tggctagtgc aacaaaagac ctaccggtta
      481 tcatggccgc tattacagac ccaattggtg ctaacttggt taaagatttg aaaaaaccag
      541 gtggcaacat tacaggggta tctgaccaca atccagctca acaacaagtt gaactcatca
      601 aggctctgac accgaatgtg aaaacaatcg gagctcttta ctcaagtagc gaagacaatt
      661 caaaaacaca ggtcgaagaa tttaaggctt atgctgaaaa agcaggtctg acagtggaaa
      721 catttgcagt tccttcaaca aatgaaattg cctcaactgt cactgttatg actagcaagg
      781 tagatgctat ttgggttcca attgataaca ccattgcatc aggatttcca acggttgtct
      841 ctagcaatca aagttctaag aaaccaattt atcccagtgc gacagctatg gtagaagtag
      901 gtggtttggc atcagttgta attgaccaac atgaccttgg tgtggcaaca ggtaaaatga
      961 ttgtgcaagt cttgaaaggt gcaaaaccag ccgatacccc agtcaatgtc ttttcaactg
     1021 gtaagtcagt catcaataaa aaaatagcac aagaactagg tattactatt cctgagtttg
     1081 ttctcaaaga agcaggacaa gtcatcgaat aaataaggta gggaggagga gactcttccc
     1141 attttttaaa aagatgaata tagaaaggat taaataaaat atgattgttt ccattatttc
     1201 tcaaggattt gtctgggcta ttctaggtct gggaatcttt atgacattta ggattttaaa
     1261 ctttccagat atgacgacag aaggttcctt ccctcttggg ggagctgttg ctgtcacttt
     1321 gataaccaaa ggcgtgaacc catttttagc gacacttgtt gctgtaggag caggttgttt
     1381 ggctggaatg gcagcaggcc ttctttatac aaaagggaag atcccaacct tgctctcagg
     1441 gattttggtg atgacttctt gtcactcaat catgctcttg attatgggac gtgcaaattt
     1501 aggcctgctt ggaaccaagc aaattcagga tgttttgcct tttgattcgg atttgaatca
     1561 actcttgaca ggtctcatct ttgtgagtat tgttattgct ctcatgctct ttttcttgga
     1621 cactaaactc ggacaagcct atattgctac aggggataat cctgatatgg ctagaagttt
     1681 cgggattcat actggacaca tggagctcat gggcttggtc ttatcaaatg gtgtgattgc
     1741 ccttgcaggt gccctcattg ctcagcaaga aggttatgcc gatgtgtctc gagggatcgg
     1801 ggttatcgtt gtggggcttg caagtttgat tattggagaa gttattttca agagtttgag
     1861 cttggcagag cgtttggtta ctatcgttgt aggttctatc gcttatcaat ttttagtgtg
     1921 ggcagttatc gcacttggct ttaatacaag ttaccttcgt ttatacagtg ccttgatttt
     1981 agcagtctgc ctcatgattc caacatttaa gcaaacaatc ttgaaaggag ccaagttaag
     2041 caaatgacag caattgtaga attaaaaaat gcaaccaaaa tcgttaaaaa tggctttgat
     2101 gaagaaaaga ttattttaaa tgatgtttcc ttagaaattt ttgaacagga ctttatcacg
     2161 attttgggtg gaaatggtgc tggtaaatca accctcttta acactattgc aggaaccata
     2221 tcactaacca gtggaactat ccgtatttta ggtgaggatg ttaccaagtt ttcaccagag
     2281 aagcgcgcta agtatctgtc tcgagtcttc caagatccaa agatggggac agctcctcgt
     2341 atgacagtgg cagagaatct cctgattgcc aagtttcgtg gtgaaaagcg tggactattt
     2401 ccgagacgtc tggctagcta taaggataag tttcaggcga ccattgaaaa agtgggaaac
     2461 ggtcttgaga aacacttgaa tacaccgatt gagttcttat ctggtggaca aagacaggct
     2521 ttgagtctct tgatggcaac cttgaagcga cctgaattac tcttgttaga cgagcatact
     2581 gctgccctgg atccaaagac tagtgttgct ttgatggaat tgacagatga atttgttaag
     2641 aaagatcaat tgacagccct tatgattacc catcatatgg aagatgctct caaatatggc
     2701 aatcgcttga ttgtcatgaa agaaggacgg gttatccaag atttgaaaca agaagaaaaa
     2761 gcaaaaatga aaatctctga ttattatcaa ctctttgaat aaagtaggga aaatgagtga
     2821 aatggtttac tttttttctg aaataaagta tactatataa agtaaactat gataacatgg
     2881 aggtattgtg tatggttgac aaacaagtca ttgaagaaat caaaaacaat gccaacattg
     2941 tggaagtcat aggagatgtg atttctttac aaaaggcagg acggaactat ctagggctct
     3001 gtccttttca tggtgaaaaa acaccttctt tcaacgttgt agaggacaag cagttttacc
     3061 actgttttgg ttgtggtcgc tcaggtgatg tctttaaatt catcgaggag taccaagggg
     3121 ttacctttat ggaggctgtc caaatcttag gtcagcgtgt cgggattgag gttgaaaaac
     3181 cgctttatag tgaacagaag ccagcctcgc cttaccaagc tctttatgat atgcacgaag
     3241 atgcggctaa attttaccat gctattctca tgacaacgac tatgggcgaa gaggccagaa
     3301 attaccttta tcagcggggt ttgacagatg aagtgcttaa acatttttgg attggtttag
     3361 cacctccaga acgaaactat ctctatcaac gtttgtctga tcagtatcgt gaagaggatt
     3421 tactggattc aggcctgttt tatctctcgg atgccaatca atttgtagac acctttcaca
     3481 atcgcattat gtttcccctg acaaatgacc aaggaaaggt cattgccttc tcaggtcgta
     3541 tctggcaaaa aacggattca caaacttcta agtataaaaa cagccgatcg actgcaattt
     3601 ttaacaaaag ttacgaatta tatcatatgg atagggcaaa aaaatcttct ggaaaagcta
     3661 gtgagattta cctgatggaa ggattcatgg atgttattgc agcctatcgg gttggaatcg
     3721 aaaatgctgt ggcgtcgatg ggaacggcct tgagtcgaga gcatgttgag catctgaaaa
     3781 ggttaaccaa gaaattggtt cttgtttacg atggagataa ggctgggcaa gccgcgatat
     3841 tgaaagcatt ggatgaaatt ggtgatatgc ctgtgcaaat cgtcagcatg cctgataact
     3901 tggatcctga tgagtatcta caaaaaaatg gtccagaaga cttggcctat ctattaacga
     3961 aaactcgtat tagtccgatt gagttctaca ttcatcagta caaacctgaa aacagtgaaa
     4021 atctgcaggc tcagattgag tttattgaaa aaatagctcc cttgattgtt aaagaaaagt
     4081 ccatcgctgc tcaaaacagc tatattcata ttttagctga cagtctggcg tcctttgatt
     4141 atgcccagat tgagcagatt gttaatgaga gtcgtcaggt gcaaaggcag aatcgcatgg
     4201 aaagaatttc cagaccgacg ccaatcacca tgcctgtcac caagcagtta tcggctatta
     4261 tgagggcaga agcccatcta ctctatcgga tgatggaatc ccctcttgtt ttgaacgatt
     4321 accgtttgcg agaagacttt gcatttgcta cacctgaatt tcaggtctta tatgacttgc
     4381 ttggccagta tggaaatctt cctccagaag ttttagcaga gcagacagag gaagttgaaa
     4441 gagcttggta ccaagtttta gctcaggatt tgcctgctga gatatcgccg caggaactta
     4501 gtgaagtaga gatgactcga aacaaggctc tcttgaatca ggacaatatg agaatcaaaa
     4561 agaaggtgca ggaagctagc catgtaggag atacagatac agccctagaa gaattggaac
     4621 gtttaatttc ccaaaagaga agaatggagt aataatggca acaaaacaaa aagaagtaac
     4681 aacatttgac gtacaggtag cagaatttat ccgtaatcat aagcaaaaag ggacagcaac
     4741 agatgatgaa atcaatgcaa gtctggttat tccttttacc ttggacgctg atgggattga
     4801 agatctcttg caacggattc aggatgcagg catttctatc acagataacg aaggaaatcc
     4861 aagtgcgcgt gttcttagca atgaagaaga accagaactc agcgatgagg acttgattgg
     4921 gtcaacttct gctaaggtca atgaccctgt ccgtatgtac ttgaaagaaa taggggtcgt
     4981 tcctctcttg accaatgaag aggagaaaga gttggcactg gctgttgaag ctggcgatat
     5041 cgaagccaaa caacgtcttg cggaagccaa tcttcgtttg gttgtttcca ttgccaaacg
     5101 ctatgtcggt cgtggcatgc agttccttga cttgattcaa gaaggaaata tgggcttgat
     5161 gaaggcggtt gacaagtttg actattctaa agggttcaag ttttcaactt atgcaacttg
     5221 gtggattcgt caggctatca ctcgtgctat tgcggaccaa gctcgtacca tccgtatccc
     5281 agttcacatg gttgaaacta tcaataaatt ggttcgtgaa cagcggaatc tccttcaaga
     5341 attggggcaa gatccgacac cagaacagat tgctgaacga atggatatga cacctgataa
     5401 ggttcgtgaa atcttgaaga ttgcccaaga accagtatct cttgaaactc ctatcggtga
     5461 agaggacgat agccaccttg gagactttat cgaagatgaa gtgattgaaa atccagtgga
     5521 ttatacgact cgtatcgtct tgcgtgagca attggatgaa atcttagata ctcttacaga
     5581 ccgtgaagaa aatgttctgc gtctacgttt tggactagat gatggaaaaa tgcgcacact
     5641 tgaagatgtg gggaaagtct ttaacgtaac tcgtgagcgt atccgtcaga ttgaagcaaa
     5701 ggctttgaga aaactacgcc aaccaagtcg cagcaaaccg cttcgtgatt ttattgaaga
     5761 ctaagagtga ggaaaatatg gcttatacag aagagcaaat tgaaaacatc aaaacacgga
     5821 ttttaacagc cttggaagaa gtcatcgacc ctgagttggg aatcgatatt gtcaatcttg
     5881 gtttgatcta tgagattcgt tttgacggtg acacagggca aacagagatt gacatgactt
     5941 tgacaactat gggttgtccc ttagcagacc ttttgacaga ccagatttat gatgctatga
     6001 tagaggttcc agaagtaacg gatactgagg ttaaattagt ctggtatcca gcctggacag
     6061 ttgaaaaaat gagtcgctat gcccgcattg ccctaggcat taagtaaaca tataaaagca
     6121 tgtgagagac tgttggaaaa gcgaggaaat ttcccctctt ttcctctagt ctctcctttc
     6181 ttttgctgat tttattcaaa gaaaatgata taatagtagt tatggagaaa aagaaattac
     6241 gcatcaatat gttgagttca agtgagaaag tagcaggaca gggagtttca ggtgcttacc
     6301 gtgaattagt tcgtcttctt caccgtgctg ccaaggacca attgattgtt acagaaaatc
     6361 ttccgatcga ggcagatgtg actcactttc atacgattga ttttccctat tatttatcaa
     6421 ccttccaaaa gaaacgctca gggagaaaga ttggctatgt gcatttcttg ccagctacac
     6481 ttgagggaag tttgaaaatt ccatttttct taaagggaat tgtgaaacgc tatgtatttt
     6541 ctttttacaa ccggatggag cacttggttg tggtcaatcc tatgtttatt gaggatttgg
     6601 tagcagctgg tattccacgt gaaaaagtga cctatattcc taactttgtc aacaaggaaa
     6661 aatggcatcc tctaccacaa gaagaggtag tcagactgcg cacagatctt ggtcttagtg
     6721 acaatcagtt tatcgtagta ggtgctgggc aagttcagaa acgtaaaggg attgatgact
     6781 ttatccgtct ggctgaggaa ttgcctcaga ttacctttat ctgggctggt ggcttctctt
     6841 ttggtggtat gacagatggt tatgaacact ataagaaaat tatggaaaat ccccctaaaa
     6901 atttgatttt tccaggcatt gtatcgccag agcggatgcg cgaattgtat gctctagcgg
     6961 atcttttctt gttgcctagt tacaatgagc tctttcctat gactatttta gaagctgcga
     7021 gttgtgaggc tcctattatg ttgcgtgatt tagatctcta taaggtgatt ttggagggaa
     7081 attatcgggc gacagcgggt agagaagaga tgaaagaggc tattttggaa tatcaagcaa
     7141 atcctgctgc cttaaaagat ctcaaagaaa aggctaagaa tatttccaga gagtattctg
     7201 aagagcatct gttacaaatc tggttggact tttatgagaa acaagccgct ttagggagaa
     7261 agtaaaaagt gaggtaatct atgcgaattg gtttatttac agatacctat tttcctcagg
     7321 tttctggtgt tgcgaccagt attcgaacct tgaaaacaga acttgaaaag cagggacatg
     7381 ctgtttttat ctttacgacg acagataagg atgtcaatcg ctacgaagat tggcaaatta
     7441 tccgcattcc aagtgttcct ttctttgctt ttaaggatcg tcgctttgcc taccgaggtt
     7501 ttagcaaggc acttgaaatt gctaaacagt atcagctaga tattatccat actcagacag
     7561 aattttctct tggcctgttg gggatttgga ttgcgcgtga attgaaaatt ccagtcatcc
     7621 atacctatca cacccagtat gaagactatg tccattatat tgctaagggg atgttgatcc
     7681 gaccgagtat ggtcaagtat ctggttagag gtttcctgca tgatgtggat ggggttattt
     7741 gccctagtga gattgtccgt gacttgctat ctgattataa ggtcaaggtt gaaaaacggg
     7801 tcattcctac tgggattgaa ttagccaagt ttgagcgtcc ggaaatcaag caggaaaatt
     7861 tgaaagaact gcgtagtaaa ctagggattc aagatggtga aaagacgttg cttagtcttt
     7921 cgagaatctc ctatgaaaaa aatattcaag cagttttagt agcctttgct gatgttctga
     7981 aagaggaaga caaggttaaa ctggtagtag ctggggatgg cccttatctg aatgacctca
     8041 aagagcaagc ccagaaccta gagattcaag actcagtcat ctttacaggg atgattgctc
     8101 ctagtgagac ggctctttac tataaagcgg cggatttttt catttcggca tcgacaagcg
     8161 aaacgcaagg tttgacctac ttggaaagct tagccagtgg aacacctgtc attgctcacg
     8221 gaaatcctta tttgaacaac ctcatcagtg ataaaatgtt tggaaccttg tactatggag
     8281 aacatgattt ggctggtgct attttggaag ccctgattgc aacaccagac atgaacgagc
     8341 ataccttatc agagaaattg tatgagattt cagctgagaa ctttgggaaa cgagtgcatg
     8401 agttttatct ggatgccatt atttcaaata acttccagaa agatttggct aaagatgata
     8461 cggtcagtca gcgtatcttt aagacagttt tgtatcttca gcaacaggtg gttgctgtac
     8521 ctgtaaaagg atctagacgc atgttgaagg cttcaaaaac acagttgatc agtatgagag
     8581 actattggaa agaccatgaa gaatagaaag aggaacagct atgaaaaaaa caattaatga
     8641 gaagcggtcg tgataaaaag attgcgggtg tttgtgctgg ggtggcccat tatctggata
     8701 tggatccgac tatcgttcaa gtcatttggg gtgttcttac ttgctgttac ggagctggaa
     8761 ttgtagctta cattatttta tggattatcg taccagtagc aactgactat tgaaaactag
     8821 ctcaattcat gctaagataa tagaaaatag aatgtaacga aggagagaaa aatggcattt
     8881 ggagataatg gaaatcgtaa aaaaactatg tttgagaaaa taaccttgtt tatcgtgatt
     8941 atcatgctag tagcaagttt attgggaatt tttgcaactg caattggtgc cctcagtaat
     9001 ctataaaata gattcaagaa aatttagtga ctgggatttc ccagcccttt tttaaagtga
     9061 gaagaaataa tgagtatgtt tttagataca gctaagatta aggtcaaggc tggtaatggt
     9121 ggcgatggta tggttgcctt tcgtcgtgaa aaatatgtcc ctaatggagg cccttggggt
     9181 ggtgatggtg gtcgtggagg caatgtggtc ttcgttgtag acgaaggatt acgtaccttg
     9241 atggatttcc gctacaatcg tcatttcaag gctgattctg gtgaaaaagg gatgaccaaa
     9301 gggatgcatg gtcgtggtgc tgaggacctt agagttcgag taccacaagg tacgactgtt
     9361 cgtgatgcgg agactggcaa ggttttaaca gatttgattg aacatgggca agaatttatc
     9421 gttgcccacg gtggtcgtgg tggacgtgga aatattcgtt tcgcgacacc aaaaaatcct
     9481 gcaccggaaa tctctgaaaa tggagaacca ggtcaggaac gtgagttaca attggaacta
     9541 aaaatcttgg cagatgtcgg tttagtagga ttcccatctg tagggaagtc aacactttta
     9601 agtgttatta cctcagctaa gcctaaaatt ggtgcctacc actttaccac tattgtacca
     9661 aatttaggta tggttcgcac ccaatcaggt gaatcctttg cagtagccga cttgccaggt
     9721 ttgattgaag gggctagtca aggtgttggt ttgggaactc agtttctccg tcacatcgag
     9781 cgtacacgtg ttatccttca catcattgat atgtcagcta gcgaaggccg tgatccatat
     9841 gaggattacc tagctatcaa taaagagctg gagtcttaca atcttcgcct catggagcgt
     9901 ccacagatta ttgtagctaa taagatggat atgcctgaga gtcaggaaaa tcttgaagaa
     9961 tttaagaaaa aattgtctga aaattatgat gaatttgaag agttaccagc tatcttccca
    10021 atttctggat tgaccaagca aggtctggca acacttttag atgctacagc tgaattgtta
    10081 gacaagacac cagaattttt gctctacgac gagtccgata tggaagaaga agcttactat
    10141 ggatttgacg aagaagaaaa agcctttgaa attagtcgtg atgacgatgc gacatgggta
    10201 ctttctggtg aaaaactcat gaaactcttt aatatgacca actttgatcg tgatgaatct
    10261 gtcatgaaat ttgcccgtca gcttcgtggt atgggggttg atgaagccct tcgtgcgcgt
    10321 ggagctaaag atggggattt ggtccgcatt ggtaaatttg agtttgaatt tgtagactag
    10381 gagactggta tgggagataa accgatatct ttccgagatg cggatggtaa ttttgtttcc
    10441 gccgcagacg tttggaatga aaagaaattg gaagaactat ttaatcgtct caatccaaat
    10501 cgtgccttga gattggcacg aactaaaaag gaaaatccat ctcagtaaag aagctaaaaa
    10561 atcccggctc tttgtcaact gtagtgggtt gaagaaaagc taagctcgag aaaggacaaa
    10621 ttttgtcctt tcttttttga tattcagagc gatgaaaatc cgttttttga agttttcaaa
    10681 gtttcgaaaa ccaaaggcat tgcgcttgat aagtttgatg agattattgg tcgcttccag
    10741 tttggcatta gaatagtgta gttgaagggc gttgacaatc ttttctttat ctttgaggaa
    10801 ggttttaaag acagtctgaa aaataggatg aacctgctta agattgtcct caataagtcc
    10861 gaaaagtttc tctggttcct tattctggaa gtgaaaaagc aagagctgat agagctgata
    10921 gtgatgtttc aagtcttccg aatagctcaa aagcttgttt aaaatctctt tattggttaa
    10981 atgcatacga aaagtaggac gataaaaacg tttatcactc agtttacggc tatcctgttg
    11041 aatgagtttc cagtagcgct tgatagcctt gtattcatgg gattttcgat gaaactgatt
    11101 catgatttgg acacgcacac gactcatagc acggctaaga tgttgtacaa tgtgaaagcg
    11161 atcaagaacg attttagcat tcgggagtga aacagtctgg gagactgttt cagcctgagc
    11221 ctagaaattt gaaagcgaag ctgtttagcc aagccatagt aagggctaaa catatccatg
    11281 gtaatgattt tgacgcgaca tcggacggct ctatcatatt taagaaagtg atctcggatg
    11341 acagcttgtg ttctgccttc aagaacagtg atgatattga gattgttaaa atcttgcgca
    11401 ataaagctca tctccatctc ccgattgaaa cagtcactcc ccggactgtt tcaacgtccc
    11461 aggacataat ctcaggaaga caagaaaaat catgtttaaa gtgaaaatca ttgagcttac
    11521 gaataacagt tgaagttgaa atggccagct gatgagcaat atcggtcata gaaatcttct
    11581 caatcaactt ttgcgcaatt ttttggttga taatacgagg aatttggtga tttttcttga
    11641 cgatagaagt ttcagcgacc atcatttttg aacagtgata gcacttgaat cgacgctttc
    11701 taaggagaat tctagtaggc ataccagttg tctcaaggta aggaatctta gacggttttt
    11761 gaaagtcata tttcttcaat tggtttccgc actcagggca agatggtgcg tcgtagtcca
    11821 gtttggcgat gatttccttg tgtgtatctt tattgatgat gtctaaaatc tggatattag
    11881 ggtctttaat gtctagtaat tttgtgataa aatgtaattg tcccatatga atctttctaa
    11941 tgagttgttt tgtcgctttt cattatagat cttatgggat tttgtggtac gacaggcatg
    12001 tatagcaaac tgaatctgga atagcacagc atatcttcta aaatattgtt ataaattgat
    12061 ttgaatttca aaattaaatt gtttgattct tatttcaatt tgttatagta tatctgatgt
    12121 caaagttctc ggcgaatcaa atagcgattc ccaagcctga ctatcgtgag gtagcggatt
    12181 aaaatggtct ggggatagac cgttttaagt ctgacgctgg aaataagaat tgtcagaaga
    12241 agggatagcg aaatcgtggc tctacgaaca ggaacgtgat aataaggcgt atatagcgga
    12301 taagagggcc tcaaactcta aagtccaaaa aggtagtcgt aacctatatg cgtaaatcac
    12361 gagagtaatt gaattcgtac taagattttc tattttcact gtaacctttt aacgtcctca
    12421 tatcttgtat acacgaggaa agatgtacga cttatcccgt gaggtctatc actataaaga
    12481 gaaaacgaca gatagaagtg atcctgagtc acggttatct ttaaaataga ttgtctgata
    12541 ggatgctagc ctgaaaacgt cttgttcatg aaatatagga cggtatgtat aaaacgcttc
    12601 tgtgaactga gagaaggggg agaagttctt gctaaaattt agttgaacag ccgtattccg
    12661 atatttagat aagagatcta gtcttagctc ctactcggtt tcaggggata aaaaaggggc
    12721 aatagcgatt cgagaaagat tatactcttc gaaaatctct tcaaatcacg tcaatatcgc
    12781 cttgccgtac gtgtaggata ctgactacgt cagttccatc tacaacctca aaacagtgtt
    12841 ttgagcaacc tgcggctagt ttcctagtgt gatctttgat ttttattgag tattagtaat
    12901 tcagttacta actcgtcaac tctgatttat ccaataaaat tgaaaaggat ggaaaaaagg
    12961 ataaatttat gatatacttt attttgaaga ccttattaga aatcttgaaa gagtattgaa
    13021 aacttagaat gagaaaaatt gttatcaatg gtggattacc actgcaaggt gaaattacta
    13081 ttagtggtgc taaaaatagt gttgtggcct taattccagc tattatattg gctgatgatg
    13141 tggtgacttt ggattgtgtt ccagatattt cggatgtagc cagtcttgtc gaaatcatgg
    13201 aattgatggg agctactgtt aagcgttatg acgatgtctt ggagattgat ccaagaggtg
    13261 ttcaaaatat tccaatgcct tatggtaaaa ttaacagtct tcgtgcatct tattattttt
    13321 atgggagcct cttaggccgt tttggtgaag cgacagttgg tctaccggga ggatgtgatc
    13381 ttggtcctcg tccgattgac ttacacctta aggcgtttga agctatgggt gccactgcta
    13441 gctacgaggg agataacatg aagttatctg ctaaagatac aggacttcat ggtgcaagta
    13501 tttacatgga tacggttagt gtgggagcaa cgattaatac gatgattgct gcggttaaag
    13561 caaatggtcg tactattatt gaaaatgcag cccgtgaacc tgagattatt gatgtagcta
    13621 ctctcttgaa taatatgggt gcccatatcc gtggggcagg aactaatatc atcattattg
    13681 atggtgttga aagattacat gggacacgtc atcaggtgat tccagaccgc attgaagctg
    13741 gaacatatat atctttagct gctgcagttg gtaaaggaat tcgtataaat aatgttcttt
    13801 acgaacacct ggaagggttt attgctaagt tggaagaaat gggagtgaga atgactgtat
    13861 ctgaagacag catttttgtc gaggaacagt ctaatttgaa agcaatcaat attaagacag
    13921 ctccttaccc aggctttgca actgatttgc aacaaccgct tacccctctt ttactaagag
    13981 cgaatggtcg tggtacaatt gtcgatacga tttacgaaaa acgtgtaaat catgtttttg
    14041 aactagcaaa gatggatgcg gatatttcga caacaaatgg tcatattttg tacacgggtg
    14101 gacgtgattt acgtggggcc agtgttaaag cgaccgactt aagagctggg gctgcactag
    14161 tcattgctgg gcttatggct gaaggtaaaa ctgaaattac caatatcgag tttatcttac
    14221 gtggttattc tgatattatc gaaaaattac gtaatttagg agcggatatt agacttgttg
    14281 aggattaaac cgtagaggtg tttatgaata tttggaccaa attagcaatg ttttcttttt
    14341 ttgaaacgga tcgcttgtat ttgcgtcctt tcttttttag tgatagtcag gacttccgcg
    14401 agatagcttc aaatccagaa aatcttcaat ttattttccc aacgcaggca agtctggaag
    14461 aaagtcaata tgcactggcc aattacttta tgaagtcccc tttgggagtg tgggcaattt
    14521 gtgaccagaa aaatcaacaa atgattggtt ctattaaatt tgagaagtta gatgaaatca
    14581 aaaaagaagc tgagcttggc tattttttga gaaaagatgc ttggtcgcaa ggatttatga
    14641 cagaggttgt tagaaaaatt tgtcagcttt cttttgagga atttggctta aaacaattat
    14701 ctatcattac ccaccttgaa aatgaagcta gccaaagagt tgctcttaag tctggattta
    14761 gtttgttccg tcagtttaag ggaagtgatc gttacacaag aaaaatgcgg gattatcttg
    14821 aatttcggta tgtaaaagga gagttcaatg agtaagcatc aggaaattct aagctatttg
    14881 gaggaattac cagtaggtaa aagggtcagt gttcgtagca tttcgaatca tctaggagtt
    14941 agtgatggaa cagcctatcg ggctattaaa gaagctgaaa accgtggaat tgtggagacc
    15001 cgtcctagaa gtggaacaat tcgtgttaaa tcccagaaag ttgctataga gagattaacg
    15061 tttgctgaaa ttgcagaagt gacttcttct gaggttctgg ctgggcaaga aggtttagag
    15121 agagaattta gtaagttttc aattggtgcc atgactgaac aaaatatctt gtcttacctt
    15181 catgatgggg ggctcttgat tgtcggagac cgaacccgta ttcagttgct agccttggaa
    15241 aatgaaaatg cagttctggt tacaggggga tttcaggttc atgatgatgt gcttaaactg
    15301 gccaatcaaa aagggattcc tgttctaaga agtaagcatg atacctttac cgtcgcgacc
    15361 atgatcaata aagccttgtc aaatgtccaa atcaagactg atattctgac agttgagaaa
    15421 ctttatcgcc ctagtcatga gtatggtttt ctgagagaga cagatacagt taaagattat
    15481 ttggacttgg ttcgtaagaa tcgtagcagc cgtttccctg ttatcaatca acatcaggtc
    15541 gttgttggcg ttgtaaccat gagagacgct ggtgataaat caccaagcac gacaattgat
    15601 aaggttatgt ctcgtagtct atttttggtt ggattatcga caaatattgc caatgtgagt
    15661 caacggatga tcgcagaaga ctttgaaatg gtaccagttg ttcgaagcaa tcaaactttg
    15721 cttggcgttg tgacgcgacg agatgtcatg gagaagatga gccgttccca agtttcggct
    15781 ctaccaactt tttctgagca gattggacaa aagctctctt atcaccatga tgaagtagtc
    15841 attacagtgg aaccctttat gctagaaaaa aatggagttt tggctaatgg tgtattggca
    15901 gaaattctga cccacatgac ccaagattta gttgttaata gtggtcgcaa tctcattatc
    15961 gagcagatgc tgatctactt tttgcaggct gttcagatag atgatatatt gcgcattcag
    16021 gcacggatta ttcatcatac gagacggtca gctataattg attacgatat ttatcatggt
    16081 caccagattg tttcaaaagc aaatgtgact gttaaaatta attagaaact aggagaaaag
    16141 atgataacat taaaatcagc tcgtgaaatc gaagctatgg acaaggctgg tgattttcta
    16201 gcaagtattc atataggctt acgtgatttg attaagccag gcgtagatat gtgggaagtt
    16261 gaagaatatg tccgccgtcg ttgtaaagaa gaaaatttcc ttccacttca gattggggtt
    16321 gacggtgcca tgatggacta tccttatgct acctgttgct ctcttaacga tgaagtggct
    16381 cacgctttcc ctcgtcatta tatcttgaaa gatggtgatt tgctcaaagt tgatatggtt
    16441 ttgggaggtc ccattgctaa atctgacctg aatgtctcaa aattaaactt caacaatgtt
    16501 gaacaaatga aaaaatacac tcagagctat tctggtggtt tagcagactc atgttgggct
    16561 tatgctgttg gtacaccgtc cgaagaagtc aaaaacttga tggatgtaac caaagaagct
    16621 atgtacaagg gtattgagca agctgttatt ggaaatcgta tcggtgatat cggtgcggct
    16681 attcaagaat acgctgaaag tcgtggttac ggtgtagtgc gtgatttggt tggtcatggt
    16741 gttggcccaa ctatgcacga agaaccaatg gttcctaact atggtattgc aggtcgtgga
    16801 ctccgtcttc gtgaaggaat ggtcttaacc attgaaccaa tgatcaatac aggcgattgg
    16861 gaaattgata cagatatgaa aactggttgg gcgcataaga ccattgacgg tggattgtca
    16921 tgtcagtatg aacaccaatt tgtcattacg aaagatggac ctgttatctt gactagccaa
    16981 ggtgaagaag gaacttatta ataaaaagtg aaaagactac tggaagttta ttatgaaaaa
    17041 atccagtaga tcttttcata ataaaacgca ttgtatcaag tgttaggggc tgatatcatg
    17101 cgtttttctg cttttaagat tttttccaac tctgtttgta agcgcatcat aacaaagggt
    17161 ctaggattca gggctctcct cctatatact attagtaaag taaaactaag ggaggatatt
    17221 ttagtgtcgc agtctattgt tcctgtagag attccacaat attgtcgttt tgattctaaa
    17281 aagagaaatg gaattctgtt taatgttcgt attgccaatc ttaaatttac cttttttaga
    17341 ttagacttcc tgcgaaacaa aatatggtat agtagttcca tgaattatga agcaagtaaa
    17401 caactaactg atgcacgatt taagcgtctt gttggtgttc agcgtaccac ttttgaagag
    17461 atgttagctg tattaaaaac agcttatcaa cttaaacacg caaaaggtgg acgaaaacct
    17521 aaattaagcc tagaagacct tcttatggcc actcttcaat atgtgcgaga atatcgaact
    17581 tatgaacaaa ttgcggctga ttttggtatt cacgaaagca acttactccg tcggagccaa
    17641 tgggttgaag taactcttgt tcaaagtggt tttacgattt caagaactcc tctcagttct
    17701 gaggacacgg taatgattga tgcgacggaa gtaaaaatca atcgccctaa aaaaagaatt
    17761 agcgaatgat tctggtaaaa agaaatgcca cgctatgaag gctcaagcga ttgtcacaag
    17821 tcaagggaga attgtttctt tggatatcgc tgtgaactat tgtcatgata tgaagttgtt
    17881 caaaatgagt cgcagaaata tcggacaagc tggaaaaatc ttggctgaca gtggttatca
    17941 agggctcatg aagatatatc ctcaagcaca aactccacgt aaatccagca aactcaagcc
    18001 gctaacagtt gaagataaag cctataacca tgcgctatcc aaggatagaa gcaaggttga
    18061 gaacatcttt gccaaagtaa aaacgtttaa aatgatttca acaacttatc gaaatcatcg
    18121 taaacgtttc ggattacgaa tgaatttgag tgctggtatt atcaatcatg aactaggatt
    18181 ctagttttgc aggaagtcta ttgaggtatt gagctagttt atgaaaaaat tgggtgaaaa
    18241 agtcgagtgt tttagaaacc cacagtgtag tattctagtt tcaatccact atattttgct
    18301 actccccgta aagtttctat tttccctgat ttctgatata atagaaatat tgacttcaag
    18361 agtaaggaag agaagatgaa cgcattatta aatggaatga atgaccgtca ggctgaggcg
    18421 gtgcaaacga cagaaggtcc cttgctaatc atggcagggg ctggttctgg aaagactcgt
    18481 gttttgaccc accgtatcgc ttatttgatt gatgaaaagc tggtcaatcc ttggaatatc
    18541 ttggccatta cctttaccaa caaggctgcg cgtgagatga aagagcgtgc ttatagcctc
    18601 aatccagcga ctcaggactg tctgattgcg accttccact ccatgtgtgt gcgtattttg
    18661 cgtcgcgatg cggaccatat tggctacaat cgtaatttta caattgtgga tcctggtgaa
    18721 cagcgaacgc tcatgaaacg tattctcaaa cagttgaact tggatcctaa aaaatggaat
    18781 gaacgaacta ttttggggac catttccaat gctaagaatg atttgattga tgatgttgct
    18841 tatgctgccc aagctggcga tatgtatacg caaattgtgg cccagtgtta tacagcctat
    18901 caaaaagaac ttcgtcagtc tgaatccgtt gactttgatg atttgattat gctgaccttg
    18961 cgtctctttg atcaaaatcc tgatgttttg acctactacc agcaaaaatt ccaatacatc
    19021 cacgttgatg agtaccaaga taccaaccac gctcagtacc aattggtcaa actcttggct
    19081 tcccgtttta aaaatatctg tgtggttggg gatgcggacc agtctatcta cggttggcgt
    19141 ggtgctgata tgcagaatat cttggacttt gaaaaggatt accccaaagc caaggttgtt
    19201 ttgttggagg aaaattaccg ctcaaccaaa accattctcc aagcggccaa cgaggttatt
    19261 aaaaataata aaaatcgccg tcctaaaaat ctctggactc aaaacgctga tggggagcaa
    19321 atcgtttact atcgtgccga tgatgagctg gatgaggctg tatttgtagc cagaaccatc
    19381 gatgaactta gtcgcagtca aaacttcctt cataaggatt ttgcagttct ctatcggact
    19441 aatgcccagt cccgtacaat tgaggaagcc ctgctcaagt ctaacattcc ttataccatg
    19501 gttggcggaa ccaaattcta cagccgtaag gaaattcgcg atattattgc ttatctcaac
    19561 cttattgcta atttgagtga caatattagt tttgagcgta ttatcaacga gcctaaacgt
    19621 ggaattggtc taggtacagt tgagaaaatc cgtgattttg caaatttgca aaatatgtct
    19681 atgctggatg cttctgctaa tattatgttg tctggtatca agggtaaggc agcccaatct
    19741 atctgggatt ttgccaatat gatgcttgat ttgcgggagc agctagacca cttaagcatt
    19801 acagagttgg ttgagtccgt cctagaaaaa acaggttatg tcgatattct taacgcccaa
    19861 gcgactctag aaagcaaggc acgggttgaa aatatcgaag agtttctttc tgttacgaag
    19921 aactttgatg acaccactga tgtgacagaa gaggaaactg gtctggacaa actgagtcgt
    19981 ttcttaaatg acttggcttt gattgccgac acagattcag gtagtcagga gacatcagaa
    20041 gtgaccttga tgaccctgca tgctgccaaa ggtctcgaat ttccagttgt ctttttgatt
    20101 gggatggaag aaaatgtctt tccacttagt cgtgcgactg aagatccaga tgaattagaa
    20161 gaagagcgcc gtctagccta tgtaggtatc acgcgtgcag agaaaattct ctatctgacc
    20221 aatgccaact cacgcttgct ttttggtcgt accaattata accgtccgac tcgttttatt
    20281 aacgaaatca gttcagactt gcttgagtat caaggtctgg ctcgtcctgc aaatacaagc
    20341 tttaaggcat catatagcag tggtagtatt tcctttggtc aaggtatgag tttggctcag
    20401 gctcttcaag accgtaaacg cggtgctgcc ccaaaatcaa tccagtcaag cggtcttcca
    20461 tttggtcaat ttacagctgg cgcaaaatca gcatctagcg aggcaaattg gtccattggt
    20521 gacattgctc tccacaagaa atggggagag ggaactgttc tggaagtttc aggtagcggt
    20581 gctaggcagg aattgaaaat caatttccca gaagtaggtt tgaaaaaact tttagccagt
    20641 gtggctccaa ttgagaaaaa aatctaattt tccatccttc tcacgaataa taaagtgagg
    20701 aggattttta tgtacagtat ttcattccaa gaagattcac tattaccaag agaaaggctg
    20761 gccaaggaag gagttgaagc gcttagtaac caagagttgc tagctatttt actcaggaca
    20821 ggaacacgtc aagctagcgt ttttgaaatt gcccaaaaag tcttgaacaa tctttcaagc
    20881 ctaacggatt tgaaaaaaat gaccctgcag gaattgcaga gtttgtctgg tattgggcgt
    20941 gttaaggcca tagaattaca agctatgatt gaactggggc atcgtattca caaacacgag
    21001 actcttgaaa tggaaagtat tctcagcagt caaaagttgg ccaagaagat gcagcaggaa
    21061 ttaggggata aaaaacaaga gcacctggtg gcactctatc tcaatactca aaatcaaatc
    21121 atccatcagc agaccatttt tatcggttct gtaactcgta gtatcgctga accgcgagag
    21181 attctgcact atgcaatcaa gcatatggcg acttctctta tcttggtcca caatcatcct
    21241 tcaggagcgg tagcgcctag ccaaaatgat gatcatgtca ctaaacttgt taaagaagcc
    21301 tgcgaattga tggggattgt tctcttggac catttgattg tctctcattc taattacttt
    21361 agttatcgtg aaaagacaga tttaatctaa agttcattaa cgacatagtc aaagagtttt
    21421 ttatctttgg gacgattttc aaaaagaagt tctggatgcc attggacacc gagaaaggcg
    21481 acatcatccg tactcatgac agcctcaatg ataccatctt taggatcatg agccacaact
    21541 tttaaatttg gtgctaagtc cttgatgctc tggtggtgga aggagttgat atgagagatt
    21601 tctccataga tttcttggag aacggtatct ggttctgtta ccaagcgttg agttgtgtac
    21661 tcaacagaag aatcctgcca atggtcttcg atatcttggt acaaagttcc acccatggca
    21721 acgttaaaga gttgggtacc acggcagaca gagaaaatgg gctttttctg tttaatagct
    21781 tccttgatga gggtcagttc gaagatatct ctttgaaggt gatagtcatc gctatcaatg
    21841 gttttgggtt cgccataaaa ttttggatcg acattttgcc cacctgtcaa gatgagcttg
    21901 tcaatcaaac tgatatagtg gcaggccatt tcttgatcac caatcggtag gatgatggga
    21961 atccctccag aatctttaac gccttcaaca aagccttttg ctgcgtagct catcatgatg
    22021 tcatcatctg gatgagcttt ttcgtttcct gtaatcccaa taactggttt tttcataaaa
    22081 tgattttcgc tttctaatcc tcttttcgca tgaagtagag gagggtttgg agttcacttg
    22141 tcaaatcgac atactgaacg accacgtctt ttggtaaatg cagatggact ggtgaaaaac
    22201 tgagaattcc tttcacacca gcatcaacca agagattagc aacctcttgt gacttgacgc
    22261 tgggaacagt taggatagca gtcttcacat cagcatcctt gattttatcc ttgatctgag
    22321 aaatcccgta aatgggaatc ccgtcaggag tttgggtacc gacttcagga tggtcgtcta
    22381 ggtcaaaggc catgataatc ttcatcttgt tacgttcgtg gaagcggtag tggagaaggg
    22441 catggcccat atttccaata ccaaccagca tgacattggt aatagagttg tcattgagca
    22501 aatcggcaaa aaatgtcatt agttttttga catcatagcc aaaaccacga cgaccaagtt
    22561 caccaaaata ggaaaaatca cgacgtacgg tcgctgaatc aataccgata gcctctgcaa
    22621 tttgcttaga gttggcacgt tcaatctttt ctgcatgaaa tctcttaaaa attcgatagt
    22681 agagagagag tctttttgct gtagcttttg gaatagcaaa ctgtttatct ttcacaaaat
    22741 cacaaccttt ctattcttct attttataga aacattgtga aaaaatcaac aaaaataaga
    22801 aaaaactaag aaaaatctta gttttgatgt aaaaaatctg catgagatag aaaacggtag
    22861 aggtctccga ccagaccctg ataaactttt tggcccctaa aagtcagaga agtcacataa
    22921 agtgtatctg gtaaggttac acatcctgac aaagtcaaca tgagagcctc atgatcctca
    22981 tacttgagag tacgctctac atgatagcag tccttatagg tcagttcaaa cattttggct
    23041 ctatctttcc gattttgtaa agacaccacg ttctaccaag ctatccatga ggaagtagaa
    23101 tttttcctga tgaatatggt ggtcttctga tttgaaaata tcaactagac gaaggccaaa
    23161 cttgtcagtg atattgattt tagcccctgt aagttccttg ttaatgatga ttttgagttg
    23221 gaagccttca ccgctgtttg gcactttttc caaaaggcga gtcagttcat agttaccaac
    23281 cttagtttca aaaaaggtgt tatctttgag ggtgaatttt ttaacagaag ggctaagagt
    23341 gtaatcgtaa cgacaatttt ttaactgaat gattttttca aatgccatat ggctaacctc
    23401 cgataatttc ttttaaggtt tttgcgaggg tttgtaggtc ttcaacggta ttttgtggcg
    23461 acaaactgat gcgaagggat tccttcaagc gttctgaatt tgcgccatac atggcttcaa
    23521 gaacatggct ggattggaca acgcctgcag tacaggctga gccagtagag attgaaattc
    23581 cagctaaatc tagccgaagg agtaagaggt catttttctg accaggaaat ccaatattga
    23641 gaacataagg gagatgatgt tttcctctat tcaagtaata ctgaatgccc tccagctctg
    23701 ccagaaaggc agtttctaga ttttgtacat gttgaaaatg ttcttcttgt ttttctaggt
    23761 cttcttttag ggctgcaacc atgcctacga tagcaggcag attttcagtt cctgcacgtt
    23821 ttttctgttc ctggtctccg ccatgtagat aggaatcaaa gtccatgcta gatgcgtaga
    23881 gaaaaccgat tcccttagga ccatggaatt tgtgggcaga agcagtgaga aaatcaatgc
    23941 ccaattcttc tgaatgaatt gggattttac caatagcctg aactgcatca acatgatagg
    24001 cagcagggtg ttgcttgagt atttggccaa tttcagcgat gggcagtagg tttcctgtct
    24061 cattattggc aaacatggta gaaaccaaaa tcgtatcgtc acgtaaagcc ttttgaattt
    24121 gctgggctgt gatttcttga ttttctggct ggataatggt tgcttcaaac ccaaagtgtt
    24181 gaaccaagta atcaattgtt tcaaggacag catggtgctc gatggcagtt gtgatgatat
    24241 gttttccttg ttcttggtga cgaagacagt agccaatgat ggtagtatta ttgccttcag
    24301 tcccaccaga agtgaaaaag atatgttgag gttttgtcct tagtaactgg gctagttcct
    24361 gacgggcttc tcgcaagagt ttgccagctt gacgaccatg accatgaata ctagaaggat
    24421 ttccgtgggt ttcttgcata accttggtca tagctgaaat agcaactgct gacataggag
    24481 tcgttgcagc attgtccaaa taaatcaaag aatcacctta tttcttttta ttgtaggcaa
    24541 agagtgggct gactggtttt ctttcgtgaa tacggacgat agcatcacca attaactcac
    24601 tagcagtgat gtagcataca tttttaggag ttttttcttt tgttgctact gaatcagtca
    24661 caagaatttc tttaatatta gtattgtcaa gaagctcagc agctccctcg acgaagagac
    24721 cgtggctaga aacagcataa atttctgtag ctccttcacg ttcaacgatt ttagaagctt
    24781 cagagaaggt acgtcctgta tttaaaatat catcaatcaa gatagctttc ttaccttcaa
    24841 catcaccaat aatataacct tcgttacgag ttgcatcgtc ttgagggtag tcgataatgg
    24901 cgataggagc atcaagatat tcagccaggc tacgcgcacg tttgacacct gaatttttag
    24961 ggctaacgac aacaacatct gaaccaagca atcctttatc gcagtaatgt tttgcgaata
    25021 ggggaacagt gaaaagatta tccactggaa tatcaaagaa accttgaacc tgaacggcat
    25081 gcaaatcaag agtcaggata cgatcaactc cagccttaac cagcatattg gcaactagtt
    25141 ttgctgtaag tggctcacga ggacaagcaa tgcggtcttg acgtgcatag ccaaaatatg
    25201 gaaggacaac gttgatactg tgggcacttg cacgcacaca agcatcgacc atgattaaca
    25261 attccattag gtggttgttg acagggaaac ttgttgattg gatgatgtaa acatcataac
    25321 cacggacact ttcttcgata tttacttgga tttctccgtc tgaaaattga cgtgatgata
    25381 gttttccaag tggggcacca acagcttggg caattttttg tgcaatctct tggttagagt
    25441 tgagtgcgaa aagtttcatg ttttttctat ctgacattat agaccgtcct ctgtaaactt
    25501 tataaatcct agttatattt accttacata tatgaactgg gatttgtgta tttttatctt
    25561 ttctatttta ccaaaaaatg gagattattt cagctatttt tcatactttt gacaaatcga
    25621 accaattttg aaggagcttt ttggtaggaa atctgatttt tctctaaaaa ttgtcggaaa
    25681 tcctgtttgc cttgctcatg attttccact tcaagctcca attcgtaatc tgttatatca
    25741 aagtatcggc tctgatccag tgccatgaga ccaatagctg ttttcatttc atagcgaagc
    25801 gttgttagac aaccaagaac ctgccagttc ttactttgga taccatgttt cgccaattca
    25861 tccagtacta gcccttgagg aagttcttcc ttactcagat agttctcagc atcttttagt
    25921 tgcaattttt ggttgtattc catgtttcca acactctgcg ggactttgag tgtcaactca
    25981 gcccagtctt caaaggttcg aatgcgcata gcgactttct tttctcgcag ttcaaaatca
    26041 ggcgtgtcga tgtagtaatt tgtttgaaga acaggagtga cacctgtgaa ctggtctttt
    26101 agacgattgt attcatcttt tttcaatagt gttttcaatt caatttctaa atgtttcatt
    26161 tttcttacct ttttttatcg ttgaaagcgg atttatggta taataagcat tgtatttatt
    26221 gtatatgaat ctggagaaaa aatcaaagat atttttgacg gataatatga gaacaaggga
    26281 gaatatatga ccttagaatg ggaagaattt ctagatcctt acattcaagc tgttggtgag
    26341 ttaaagatta aacttcgtgg tattcgtaag caatatcgta agcaaaataa gcattctcca
    26401 attgagtttg tgaccggtcg agtcaagcca attgagagca tcaaagaaaa aatggctcgt
    26461 cgtggcatta cttatgcgac cttggaacac gatttgcagg atattgctgg cttacgtgtg
    26521 atggttcagt ttgtagatga cgtcaaggaa gtagtggata ttttgcacaa gcgtcaggat
    26581 atgcgaatca tacaggagcg agattacatt actcatagaa aagcatcagg ctatcgttcc
    26641 tatcatgtgg tagtagaata tacggttgat accatcaatg gagctaagac tattttggca
    26701 gaaattcaaa ttcgtacttt ggccatgaat ttctgggcaa cgatagaaca ttctctcaac
    26761 tacaagtacc aaggggattt cccagatgag attaagaagc gactggaaat tacagctaga
    26821 atcgcccatc agttggatga agaaatgggt gaaattcgtg atgatatcca agaagcccag
    26881 gcactttttg atcctttgag tagaaaatta aatgacggtg taggaaacag tgacgataca
    26941 gatgaagaat acaggtaaac gaattgatct gatagccaat agaaaaccgc agagtcaaag
    27001 ggttttgtat gaattgcgag atcgtttgaa gagaaatcag tttatactca atgataccaa
    27061 tccggatatt gtcatttcca ttggcgggga tggtatgctc ttgtcggcct ttcataagta
    27121 cgaaaatcag cttgacaagg tccgctttat cggtcttcat actggacatt tgggcttcta
    27181 tacagattat cgtgattttg agttggacaa gctagtgact aatttgcagc tagatactgg
    27241 ggcaagggtt tcttaccctg ttctgaatgt gaaggtcttt ctcgaaaatg gtgaagttaa
    27301 gattttcaga gcactcaacg aagccagcat ccgcaggtct gatcgaacca tggtggcaga
    27361 tattgtaata aatggtgttc cctttgaacg ttttcgtgga gacgggctaa cagtttcgac
    27421 accgactggt agtactgcct ataacaagtc tcttggcggt gctgttttac accctaccat
    27481 tgaagctttg caattaacgg agattgccag ccttaataat cgtgtctatc gaacactggg
    27541 ctcttccatt attgtgccta agaaggataa gattgaactt attccaacaa gaaacgatta
    27601 tcatactatt tcggttgaca atagcgttta ttctttccgt aatattgagc gtattgagta
    27661 tcaaatcgac catcataaga ttcactttgt cgcgactcct agccatacca gtttttggaa
    27721 ccgtgttaag gatgccttta tcggtgaggt ggatgaatga ggtttgaatt tatcgcagat
    27781 gaacatgtca aggttaagac ctttttaaaa aagcacgagg tttctaaggg attgctggcc
    27841 aagattaagt ttcgaggtgg agctattctg gtcaataatc aaccgcaaaa tgcaacgtat
    27901 ctattggacg ttggagacta cgttaccatt gacattcccg ctgaggaagg ctttgaaacc
    27961 ttggaggcta ttgagcatct attagatatt ctctatgagg atgatcactt tctagtcttg
    28021 aataaaccct atggagtggc ttctattcct agtgtcaatc actctaatac cattgccaat
    28081 tttatcaagg gttactatgt caagcaaaat tatgaaaatc agcaggttca cattgttacc
    28141 agactagata gggacacttc tggcttgatg ctctttgcca agcacggtta tgcccatgca
    28201 cgattagaca agcagttgca gaagaaatct atcgagaaac gctactttgc tttggttaag
    28261 ggagatggac atttggagcc agaaggggaa attattgctc cgattgcgcg tgatgaagat
    28321 tccattatta ccagacgagt ggctaaaggc ggaaagtatg cccatacttc atacaagatt
    28381 gtagcttctt atggaaatat tcacttggtc tatattcacc tgcacactgg tcgaacccat
    28441 caaatccgag tccatttttc tcatatcggt tttcctttgc tgggagatga tttgtatggt
    28501 ggtagtctgg aagatggtat tcaacgtcag gctctgcatt gccattacct atccttttat
    28561 catccatttt tagagcaaga cttgcagtta gaaagtccct tgccggatga tttcagtaac
    28621 cttattaccc agttatcaac taatactcta taaaaactgt ctcagagtat aattattatc
    28681 ttaaaggaga aaactcatgg aagtttttga aagtctcaaa gccaaccttg ttggtaaaaa
    28741 tgctcgtatc gttctccctg aaggggaaga gcctcgtatt cttcaagcaa caaaacgctt
    28801 agtaaaagaa acagaagtga ttcctgtttt gcttggaaat cctgaaaaaa ttaaaattta
    28861 tcttgaaatt gaaggaatca tggatggtta tgaggtcatc gaccctcaac attatcctca
    28921 atttgaagaa atggtttctg ccttggtgga gcgtcgcaag ggcaaaatga ctgaagaaga
    28981 tgtacgcaag gttttggttg aagatgtcaa ctactttggt gtgatgttgg tttacttggg
    29041 cttggttgat ggaatggtgt caggagcgat tcactcaaca gcttcaacag ttcgtccagc
    29101 tctacaaatc atcaaaactc gtccaaatgt aactcgtact tcaggagcct tcctcatggt
    29161 tcgtggtacg gaacgttacc tatttggaga ctgtgccatt aacatcaatc cagatgcaga
    29221 agccttggct gaaattgcca tcaactcagc aatcacagct aagatgtttg gcatcgaacc
    29281 taaaattgcc atgttgagct attctactaa aggttcaggg tttggtgaaa gcgttgataa
    29341 ggtcgttgaa gcaactaaaa ttgctcacga cttgcgtcct gaccttgaaa tcgatggtga
    29401 gttgcaattt gatgcagcct ttgttcctga aactgcagct ctgaaagctc ctggaagtac
    29461 ggtagctggt caagcaaatg tcttcatctt cccaggtatc gaggcaggaa atatcggtta
    29521 caagatggct gaacgcctag gtggctttgc ggctgtagga cctgttttgc aaggtttaaa
    29581 caagccagtt aatgatcttt ctcgtggatg taatgcagat gatgtttaca agttgaccct
    29641 catcacagca gctcaagcag ttcatcaata gtgaaaacta taaagcgata tactatgcta
    29701 tactgtagtt atgaaactat gtacgaaaag cactgccatt aattcctgag aactaaatta
    29761 ctgattggtg tcaaaaagga aaacttccaa gcgataatat cctgtctata cacgacctat
    29821 agaaatctgt aatatacatg tccgtaaaac gataaattcc ctttttgatt ttaaatgagt
    29881 atgaaaagag a
//
LOCUS       DAVKQB010000028        27925 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKQB010000028 DAVKQB010000000
VERSION     DAVKQB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27925
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.028"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..153)
                     /locus_tag="VW528_001219"
     CDS             complement(<1..153)
                     /locus_tag="VW528_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912040.1"
                     /translation="MLSKEEYIEEIGLIEKQNYVEVELYPLVADIINPTLKNSLSKRY
                     VFGRRKS"
     gene            complement(328..1263)
                     /locus_tag="VW528_001220"
     CDS             complement(328..1263)
                     /locus_tag="VW528_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9912041.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTNWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSYHRS"
     gene            complement(1265..2284)
                     /gene="galE"
                     /locus_tag="VW528_001221"
     CDS             complement(1265..2284)
                     /gene="galE"
                     /locus_tag="VW528_001221"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9912042.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            complement(2585..3688)
                     /locus_tag="VW528_001222"
     CDS             complement(2585..3688)
                     /locus_tag="VW528_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9912043.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            complement(3705..4502)
                     /locus_tag="VW528_001223"
     CDS             complement(3705..4502)
                     /locus_tag="VW528_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9912044.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLEIEETTYLQETGPERGIGRIGNIQPQTFGELAQHVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            complement(4489..5166)
                     /locus_tag="VW528_001224"
     CDS             complement(4489..5166)
                     /locus_tag="VW528_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9912045.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLCKEVSPVFVQKWQKEAVKLEFALGQIPDKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            5715..6183
                     /locus_tag="VW528_001225"
                     /pseudo
     CDS             5715..6183
                     /locus_tag="VW528_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6177..6707
                     /locus_tag="VW528_001226"
     CDS             6177..6707
                     /locus_tag="VW528_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEU9912046.1"
                     /translation="MLDEKRKNYVEDKRQNFPSLPSWKMDLFSEEEKRIYFQTTCEVS
                     SKDSAINKYKMEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDAEGEWTALDDI
                     KNEAHMLKKLADKSYTTNLTDEFYIVDDYFLVQEQVDGLNFEEFIRETEHSLNIREKT
                     LDNIVNIVSYIHKLGI"
     gene            6781..8217
                     /locus_tag="VW528_001227"
     CDS             6781..8217
                     /locus_tag="VW528_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9912047.1"
                     /translation="MTPIKDKVRRVKTPMMVNPDTDLTISSVQQDYFSLALIGFSLLT
                     GDFLSFSKGDQKTGLSAFIKICHLIKIARLDNKITKQQEYWLYDLLMMSQGEKIQKIK
                     QLKQVTSDILLNTPDFSSYFEKYNFKEEAENIKSYLLAKSMDKSGRLFPSNEFGEFVS
                     PVSFQHGFGGVLFFMNKYYVEEDENTVKEWLTKLENYEAANFLHGYSLLFGKAGFLFG
                     ILDRYEKTKERYLIDISKRLVDHLMRVYDNISNLDFALGKSGILLSLMKYCTIFDDKK
                     LANFIKNNINDAYSLLESEDNGDIYSNNFAHGRSGAAYVLKAYTDIFGDSRYQNHLQK
                     FSDGISELLEEKLSSFSKLDNLGLSWCDGVSGLILYLCLIDKERYSEIIYKSQLEMVQ
                     QYEAMGTSFCHGLSSLLQTTIYNKNQKVEQFIKKILLTRSYRNNDRLLQFQGEDGINS
                     YFDFGVGNLGIYWTLLGYTFPFELSKGD"
     gene            8219..9457
                     /locus_tag="VW528_001228"
     CDS             8219..9457
                     /locus_tag="VW528_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912048.1"
                     /translation="MHIFLKNRAFRQLTVNEWISSFGDTIFYLAFINYVSSYAFAPLA
                     IFLISLSETIPQVLQLFTGVIADFQKNRISKYISILFIKVLLYSGVTLLLTSTDFSLF
                     SVFFICSMNLISDTIGFLAGYMLTPIYIRLINDDMTEAMGFRQSTSSIVRLIGNLSGG
                     VFLGLFSISTLAFVNVLTFLFAFLGSLLIRNRLKKEEEKIEVPPYVGMSSFFQHLKES
                     MKLLMTMEDVMVLLWILSISQAVLMMVEPVSAILLIHHPFMGLSTGQSLAILIMISLL
                     HVILGGLLSGFLSKKISIRLNIYWSLLMESLIVIDFLRGSFLLILLGSAGDAFSAGVL
                     SPRLQAMIFGIIPEELMGSVQSSINVINLLIPAVLSLALVFLATSAGLEVVAFALIIL
                     LLIAAYLVHQMKNLPNQEEV"
     gene            9646..12792
                     /locus_tag="VW528_001229"
     CDS             9646..12792
                     /locus_tag="VW528_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEU9912049.1"
                     /translation="MIRTEVTSMTVPDRLPARMRMDGRSIFQQIKNYFDTENKEYFKH
                     PNTYDGISMHLEPNILTSMEHFDLTGFHCECKDFQNQGVCKHWVAMDLYFRSLPQAIQ
                     ERIGKASHKPSFASQLIPTLPSEELQDELVEEKATAPSLALHGQVEIRNHSLAWTLKL
                     QVEQAPRAYVIKDIAHFIFLIFQKEDYFVSQKIGTIRLSLNQFNQASQNLLLYIKKYF
                     IDRNEHSYFNFSYGINPRDYGRYLETPVSYLNDLVPLFQALDVFQYVTSKAEYPLIFL
                     DDSPFIPEEEIFKVVKSNNHYEIINTAYFGFIIQEKLWIRHNHFHIIKEEHRYFLDKL
                     ATWIYHYQENSPLIFSKENKAELMQVCNIISNYVPISIPDELQIHDFIPTFAFSKTRN
                     EIALNMVWSFGEKQVHSKQDLLTLPYTYQASKARKIYHQLLSAGFKEEFHSLSKIKIV
                     DFFLKELPRFRTLGQVQLDESLEKLLVEDPAVIDIFDDESFLSVQFDFSMISEDEVEK
                     AIQALWNQESHYQTKQGKVLVFDDESLKVAQSLQDLRAKFSDGKIKMHKSRAFSLSET
                     FKDNEHVNFSRDFKKMAYDLTHPEEFDIKPYEVKAKLRSYQKEGVKWLSMLDHYHFGG
                     ILADDMGLGKTLQTITLLEANLKPDQKALILAPASLLYNWKEEFRKFVPHKQVEVAYG
                     SKTERIKQIEKSATITITSYPSFRSDLEHYQKQSYDYLILDEAQMIKNSQTKTAQALR
                     EFDVKTCYALSGTPIENRLEEIWSIFQIVLPGLLPSKKEFSKLSPQLVAKLIQPFVLR
                     RKKDEVLTELPELSEHLYSNELSSSQKTLYLAQLRRMQEMVSGASAYEIKRHKIEILA
                     GLTRLRQICNTPALFLEDYKGDSGKMDSLFELLDTIREKGSRPLIFSQFTSMLDLIEQ
                     ELEKKEMSHFKITGQTPSDKRQEMVNLFNQGEKDCFLISLKAGGTGLNLTGADTVILC
                     DLWWNPAVEMQAIGRSHRLGQTKQVDVYRLITLGTIEEKIQELQESKKELFNTVLEGQ
                     ESRSNLSVDDIKEILGVE"
     gene            complement(13229..15565)
                     /locus_tag="VW528_001230"
     CDS             complement(13229..15565)
                     /locus_tag="VW528_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9912050.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFGVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEIATLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            15698..16447
                     /locus_tag="VW528_001231"
     CDS             15698..16447
                     /locus_tag="VW528_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9912051.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            complement(16740..17354)
                     /locus_tag="VW528_001232"
     CDS             complement(16740..17354)
                     /locus_tag="VW528_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9912052.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIQRL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(18169..18438)
                     /gene="rpsO"
                     /locus_tag="VW528_001233"
     CDS             complement(18169..18438)
                     /gene="rpsO"
                     /locus_tag="VW528_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9912053.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(18579..19208)
                     /locus_tag="VW528_001234"
     CDS             complement(18579..19208)
                     /locus_tag="VW528_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9912054.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADEFDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNGKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            20067..20390
                     /locus_tag="VW528_001235"
     CDS             20067..20390
                     /locus_tag="VW528_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9912055.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            20448..22391
                     /gene="thrS"
                     /locus_tag="VW528_001236"
     CDS             20448..22391
                     /gene="thrS"
                     /locus_tag="VW528_001236"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9912056.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(22440..23414)
                     /locus_tag="VW528_001237"
     CDS             complement(22440..23414)
                     /locus_tag="VW528_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9912057.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(23407..24084)
                     /locus_tag="VW528_001238"
     CDS             complement(23407..24084)
                     /locus_tag="VW528_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9912058.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            24240..25313
                     /locus_tag="VW528_001239"
     CDS             24240..25313
                     /locus_tag="VW528_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9912059.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            complement(25404..25706)
                     /locus_tag="VW528_001240"
     CDS             complement(25404..25706)
                     /locus_tag="VW528_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912060.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(25775..26212)
                     /locus_tag="VW528_001241"
     CDS             complement(25775..26212)
                     /locus_tag="VW528_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9912061.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(26288..27130)
                     /locus_tag="VW528_001242"
     CDS             complement(26288..27130)
                     /locus_tag="VW528_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9912062.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            27242..27892
                     /locus_tag="VW528_001243"
     CDS             27242..27892
                     /locus_tag="VW528_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9912063.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
BASE COUNT         8647 a         6010 c         5111 g         8157 t
ORIGIN      
        1 actctttctt cttccgaaga catatctttt tgataaacta ttttttaaag tcggattaat
       61 tatatcagca actaaaggat acagttcaac ctctacataa ttttgtttct ctataagccc
      121 gatttcttca atatactcct ctttacttag cataaaataa ccctcctgaa ttttttattt
      181 attaagttat cgtctttgca gtaaatttat tccacatagt tcatttatat catgtcaaat
      241 ccctaatttt tttaaaaatc actcaagttc cagaccaaaa gaattataat tacaaaaaag
      301 tgactgcgct agataactcc tactttttca tgatctatga taactttttt ctttgataag
      361 ataaagcggt ctttttttgg tttctaaata aatcttactg atatacttgc cgagaatccc
      421 aatggtcaag agttgaatac ctccaagaaa gagaataaca gccatcagag aggtccaacc
      481 agatgtcgga ttgcccaaaa tgagggtccg aaccacaaca aaaaaggtca tcagcagaga
      541 aagaaaacaa gataggagac cagctacaaa ggctataatc aagggaaaat ctgaaaaatt
      601 aataatccct tcaatggagt agaaaaagag ttgcctaaaa ctccaatttg tcttgccagc
      661 ctgcctttcg acatttggat agtccaaata gtaggttttg aaacccaccc aggcgaagag
      721 cccctttgaa aaacgattgg actcggtcaa gcttaaaatg gcatcgacta cagaccttct
      781 catcatacga aaatcacgga cacccgacgg cagagctact gggctgattt tttgcatgag
      841 gcgataaaag agaacagcac agaaactgcg aaagaagggt tctccctccc gactagttct
      901 ccgtgtccca acgcagtcca agtctacatt tttgtctagt acatttttca tctcaaacaa
      961 catactagga ggatcttgga ggtctgcatc catcaccacc accaaatctc ctgtcgcata
     1021 ttgcaagcct gcataaaggg ctgcttcttt gccaaaattt cgagagaaag aaatataatg
     1081 gactgccgga ttttgctccc gataggcctt taagagttcc aaggtcccat cacttgatcc
     1141 atcatcgaca aagacatact cgatttctgt ttccaaatct ggaagtaaag cttccagagc
     1201 ctgataaaaa agaggaagta cttcctcttc gtttaaacaa gggacgatga ttgaaatcat
     1261 catcttagtc ttcaaatcca tttggatgct tgctttgcca acgccatgcg tcttcacaca
     1321 tttgggtgat gtcgagttct gcttcccaac cgagttctgc tttagctttt gctgggtctg
     1381 agtagcaggc agcgatatca cctgggcgac gttctacgat acggtaagga ataggacgtc
     1441 ccaccgcttt ttccatgttt tggataattt caagaactga gtaaccttta ccagttccaa
     1501 ggttataaac gtttagtcct gaaccttttt ggattttttt caaagctgca acgtgaccct
     1561 tagccaaatc gacaacgtgg atatagtcac gaacacctgt tccatcttcc gtatcgtaat
     1621 cgtctccaaa cacttgcact tgctctaatt ttccaacggc tacttgagtc acatatggca
     1681 agagattgtt tggaataccg tttggatttt ctcccaaatc accactctca tgggctccga
     1741 ttgggttaaa gtaacgaagc aagacaacat tccattctga gtctgctttg taaatatcgg
     1801 tcaaaatttc ctctagcatg agcttggtac gaccgtatgg gttggtcact gaaagtggga
     1861 aatcttccaa gatgggcact gtgtgcggat ccccgtaaac tgtcgcagaa gaactgaaga
     1921 tgatgttttt acagttgttt tcttccatgg ctttcaaaag gctgacagtt ccagcgatat
     1981 tgttgtcata gtaggcaaga gggatacgtg ttgattcgcc aacagccttc aaaccagcaa
     2041 agtgaatgac accagtcggt tcttcctgct tgaaaatatc tctgagggta gctgtgtcac
     2101 gaatatctgc ctcatagaaa ggaatctcaa ctcctgtaat tctttcaaca acttctaaac
     2161 tcttacgatt gctattgaca agattatcca ccacaacaac ttgatggcct gcttggatca
     2221 attcaataac agtgtgggtt ccaataaaac cggcaccacc agttaccaaa atcttttctt
     2281 gcatcttttt tcctcgattc tcagattatt ttttcttatt ttaccatttt tgacagggaa
     2341 tgtcatttgc catcctaaac tacctgataa aatttcagta aaatgcttat actcttcgaa
     2401 aatccaattc aaaccacgtc aacgtcgcct tgccatgggt atggttactg acttcgtcag
     2461 ttctatctac aacctcaaaa cagtgttttg agctgacttc gtcagttcta tccacaacct
     2521 caaagcagtg ctttgagtaa cccgcggcta gttacctagt ttgttctttg attttcattg
     2581 agtattattc gctttttact cgtttgacat agttttcaat tgggtagttt tcagggtcca
     2641 aggtcaactc cttgtcttgg atcagttgag ctaaatggta accaataata ggaccagttg
     2701 tgaggcctga tgaacctaat ccactggctg catagacacc agttaagtca ggcacctgcc
     2761 caaagaaagg agagaaatca ctggtgtagg cacggattcc aacacgctca gattttgaag
     2821 tagcttcagc caaaatcaga tagtgagtca aggtggcctc ctccatttgt tggagcaagg
     2881 tttcatctac cgtcaaatca aatcccatgt cattttcgtg ggtagcgcct aaggacaatt
     2941 tcccacctgc aaaaggaatc aaatcccact ccccttctgg catgacaaca gggtaagctt
     3001 ccatatcttg gaccagctga taatctcgta gttgtccttt ttgaggacga acatctactt
     3061 cataacccaa gggttctaac atgtccccca accaagctcc cgtcgccaaa ataacctgct
     3121 caaactcctc ttcaccaatc tggtagcctg atgctaacgg tgtcagagtc actttttctt
     3181 tgaccagctt gacatgactg gcttctagca gacgagttac caagagttgg ccatctactc
     3241 tcgctccacc agaagcatag agcaggcggt caaatccctg caaaccaggg aataattcat
     3301 tagctgaggc ttggttcaga atggctaatt gccctatcaa gggagattct tctctgcgct
     3361 ggagggccag ttgataaagt tcttccaaat tggattcatc ctttttcaag agaaagactc
     3421 ccgaacgctg gtaaaagtcg atttcttgtc ctgatttctc taaatcagct aataaatcca
     3481 cataaaaatc agcccccaag cgcgccatct tgtaccaggc tttattacgg cgtttggaaa
     3541 accaaggact gataattcct gctgcggcct tggtggcttg accttgctca tggtcaaaaa
     3601 cggtcacctc taggtcactt tctctcgaga ggtagtaggc agctgttgct cccacaattc
     3661 ctgctccaat aatggcaact tttttcattg tcttcacttt ctaactagat atgacggaaa
     3721 ggattggtcg atgcttgact aggtaaaatc tctagttccc agcctttttt ctctttccat
     3781 tgagtaagga gtgctgcgat tttttccaca aaaagcactt cgatatagtg acctggatcc
     3841 aatgctaaca agccatcaga cagcatatcc tgagcagtat ggtagtagat atcaccagtg
     3901 atatagacat ctgccccctt tgccaaagca tccttataga aagactgccc gcttccacca
     3961 caaattgcta ctcttgaaat aggcttctgc aaatcattct cttgataatg caccattcga
     4021 aggctatcta ggtcaaagac ttgcttgaca tgttgggcca attctccaaa ggtctgaggc
     4081 tgaatattcc caatacgtcc aattccacgt tctggacctg tttcctgcag ataagtcgtc
     4141 tcctcgattt ctagcatctg acaaaaccag tcattgagcc cattttcaac gatatcaata
     4201 ttggtatggc tgacataaac tgcgatgtca tgcttaatca ggtcgatgta aatctgattt
     4261 tgcggacggc tggcaagcaa gtccttgata ggacgaaaga taggcgcgtg cttgacgata
     4321 atcaagtcca cacccttttc aatggcctct gccactgtct cttcacgaat atcgagggca
     4381 accatgaccc tttggatacc cttgtctaaa gtgccaattt gcagaccacg gctgtctccc
     4441 tccatagaaa attcctgagg gcaaaaggct tcataagctt ggatcacttc acttgctaac
     4501 atggagcacc tccttgatgg cttgaatctt atctactaga acttgacgtt cttccagatt
     4561 tttgtctggg atttgtccga gggcaaactc tagcttaaca gcttcttttt gccatttttg
     4621 gacaaagact ggactgacct ctttgcacaa gaacggtcca aagcgaacat cactggctga
     4681 tagcttcatt tgtcctgctt ccaccaccaa aatctcataa aactttccag cttcttctaa
     4741 gatgctttct gctacaatct ggaatccatg atcctgtagc cagatacgca agtcgtcttc
     4801 acgattattg ggctggagga tcaaacgctc tacattagct aacttcccca aaccttcttc
     4861 taaaatccta gcaatcaaac gaccacccat gccagcaatg gtaatgacag acacttggtc
     4921 agtctcttca aaagctgcca agccattggc taaacggact tggattttct cctttaggcc
     4981 gtgagcctca acatttttaa ccgcagactg atagggacct tccaccacct cacctgcaat
     5041 agcgcttttg atttggcctc tctcaaccaa ctcgataggc agataagcat ggtcacttcc
     5101 cacatctagt aaaatagccc cctgtgacac aaaggaagct accaattcta atctctttga
     5161 aatcatcttc tctcactttc caaaactcta ttacctctta ttataccaca tttcaatctt
     5221 caacttccca gtaatataag cacctctggc gaaagaagtt tcaatgtcct aaagtaataa
     5281 gtgaatccaa ttgaaagatt ttaaacaatt tgcaaaaatg tcaaaaaata aaaaataaac
     5341 agtttattca gaaaattctt gacatataaa aacacatggt agaatataat tagaaagtta
     5401 gaaaaaataa aagtttgact aaaatttgta tttgaaggtg gtgttcagat aagaaattta
     5461 gtcagacgaa ccacgaattt gctctatgct ttctggaatt tatcataaca ggaggataca
     5521 gtcatggaac aaacattgtt tgaattagaa ctacttccag aggaagatat cattgtcaca
     5581 ggtctcccta agtattgttc ttttacttgt ttaattacag gtcgctagtt atattttata
     5641 taaaataagt agctttactt acggaatagg ctagtgctgt gtctctagcc tattttaata
     5701 attaggagtt tgttatggat ttattagaga aagaatgttt aaaatgtgat aaaaatttcc
     5761 aacagggtga tatttggaat tactattatt tatcagataa gatgcctgca caagggtgga
     5821 aaatacacat aagctcccaa ataaaagacg ctgtaaatat ttttaagatt gtgtataaac
     5881 tatcccaact aaataattgt agctttaaag ttgttaaaaa tttagaggaa ttaaaaaaaa
     5941 ttaattcccc tagggaaatg agccctactg ctaacaaatt tataactcta tatcctaagt
     6001 cagaatctga agctaagagt atgatttgta atcttacgaa tagactgtca gaatttaagg
     6061 ctccaaaaat actatctgac tatcaatgtg gaatgcattc tccagttcat tatagatatg
     6121 gggctttttt aaaaaaacaa gcttatgatg aaaaaaataa aaaagtcatc tatttattgc
     6181 tagatgaaaa aaggaagaac tatgtagaag ataagagaca aaatttccct agtcttccta
     6241 gctggaaaat ggatttattt tcagaagaag aaaaaaggat ttattttcaa accacatgtg
     6301 aagtttcaag taaggactct gcaataaata aatacaaaat ggaaaaaatt attaagagat
     6361 caaataaagg aaatgtttat cgggcaatta gaaaatcgga cgggcaaaaa gtaatcatca
     6421 agcagtcaag accttttgtg aattatgatg ctgaaggaga atggacagct cttgatgata
     6481 taaaaaatga agctcacatg ctaaaaaaat tagccgataa aagttatact actaatttaa
     6541 ctgatgaatt ttatatcgtg gatgattatt ttcttgttca ggaacaagtt gacgggttaa
     6601 actttgaaga atttattcga gaaacagaac actccttaaa tattagagaa aaaacactgg
     6661 ataacattgt gaatatagta agttacatcc ataaactggg aatataaaat tgtagatata
     6721 gctcctacaa attttatata taccaaaaaa ggtgatataa cattaataga cttggagaca
     6781 atgactccta tcaaggataa ggtaagacga gttaaaacac caatgatggt taatccagat
     6841 actgacctaa ctatcagctc agttcaacaa gattatttta gtttagcact aattggattt
     6901 tctttattaa caggtgattt tttatccttt tcaaaaggtg atcaaaaaac aggattatcg
     6961 gcctttataa aaatatgtca tctaataaaa atagctagat tagacaataa aataactaaa
     7021 caacaagaat actggctata cgatttatta atgatgagcc agggagaaaa aatccaaaaa
     7081 ataaagcaat tgaaacaagt tacaagtgac attcttttaa atactccaga tttttcaagt
     7141 tattttgaaa aatataattt taaagaggag gctgagaata taaaatcata cttactggct
     7201 aaatctatgg acaaatctgg tcgtttgttt ccgtcaaatg aatttggtga gttcgtaagt
     7261 cctgtttctt tccaacatgg ttttggagga gttttatttt ttatgaataa atattatgtt
     7321 gaggaagatg aaaataccgt taaagaatgg cttacgaaat tagaaaatta tgaagcagct
     7381 aattttttac atggttactc tctactattt gggaaagcag gtttcctatt tggaatccta
     7441 gataggtatg aaaaaacaaa ggagagatat ttaatagaca taagtaagag attagtcgat
     7501 catttaatgc gggtttatga caatattagt aacttagatt ttgctttagg gaagtctggt
     7561 atcctactca gtttgatgaa atattgtacc atttttgatg ataaaaaact agcaaatttt
     7621 atcaagaaca atataaatga tgcatatagt ttattagaat ccgaggataa tggagatata
     7681 tattctaata attttgctca cggacgttca ggtgctgcct atgtattaaa agcctatact
     7741 gatatttttg gagatagtcg ctatcaaaat catttacaaa aattttctga cgggatatct
     7801 gaattactag aagagaaact ttcaagcttt tctaaattag ataatttagg tctttcttgg
     7861 tgtgacggag tttcaggatt aatattgtat ctttgtttga ttgataaaga aagatactct
     7921 gagataatct ataaatcaca acttgaaatg gtgcaacaat atgaagcaat gggaactagt
     7981 ttttgccatg gtttaagtag tttattgcaa acaacaatat acaataaaaa tcaaaaggtt
     8041 gaacagttca taaaaaaaat cttattgact cgttcttatc gtaataatga tagattatta
     8101 caatttcaag gggaagatgg aataaacagt tattttgact ttggtgtggg aaatctgggt
     8161 atttattgga ctttactcgg ttatacattc ccatttgaat taagtaaagg agattaatat
     8221 gcatattttt cttaaaaatc gagcttttcg tcagctaaca gtaaatgagt ggatttcaag
     8281 ttttggagat actatttttt acttagcttt tatcaattat gtctcttctt atgcctttgc
     8341 tcccttagct atatttttga ttagcctatc agaaaccatt ccacaggtct tgcaactttt
     8401 tactggagtt atagctgatt ttcaaaaaaa tcgaatcagc aagtatattt ctatcctctt
     8461 tatcaaggtc ctcctttatt ctggagtgac tttgctcctg acaagtaccg acttctctct
     8521 cttttctgtt ttctttattt gcagcatgaa cttgatttca gatacgattg gttttcttgc
     8581 aggatacatg ctgacaccga tttacatacg actaataaac gatgatatga cggaggctat
     8641 gggttttagg cagtccacga gttccattgt acgtttgata ggtaatctca gcggaggagt
     8701 atttttagga ctgtttagca tttctactct agcttttgta aatgttttaa cctttttatt
     8761 tgcctttcta ggaagtctac ttatccgaaa ccgcttgaaa aaagaggaag aaaaaatcga
     8821 agttcctcct tatgtaggaa tgagtagctt tttccagcat ttgaaagagt caatgaaatt
     8881 attgatgact atggaagatg tcatggtctt gctttggatt ttgtccatta gtcaagctgt
     8941 tttgatgatg gtagagcctg tcagtgcgat tctactgatt caccatcctt ttatgggttt
     9001 gtcaacaggg caaagtctgg caattttaat aatgataagc cttcttcatg ttattttagg
     9061 tggacttctg agtggatttc tatctaaaaa aatttctatt cgcctgaata tttattggag
     9121 tctattgatg gagtctctca ttgttataga ttttctacgt ggaagttttt tacttatttt
     9181 actaggaagt gcgggtgatg ctttctcggc aggagtattg agtcctcgct tgcaagccat
     9241 gatttttgga atcattcctg aggagttaat gggatcagtt caatcatcca tcaatgttat
     9301 caacttactt atcccagctg tcctatccct tgctctcgta tttcttgcaa caagtgctgg
     9361 tctggaagtt gttgcttttg ctcttataat tttactactt atagcagcct atctagttca
     9421 ccagatgaaa aacttaccaa accaagaaga agtttagcta gaatgttatg gataagtcag
     9481 ttttcacttg atagtttcta tggaatcgta atctccttgt aattgattgt cgatattaca
     9541 tacaactatc ttgcaactac aaatgtttcc aatccctatc aagatgcaat ttatgtaaga
     9601 taaatctatt cttttcctat aaattctgat aagatagaga aaagaatgat tagaacagag
     9661 gttacttcaa tgacggttcc cgatagacta cctgctcgta tgagaatgga tggccgctct
     9721 attttccaac agataaaaaa ctattttgat actgaaaata aggaatattt taagcacccc
     9781 aatacctatg atggtatttc catgcatctg gagcccaaca tcctgaccag tatggagcac
     9841 ttcgatttga ctggctttca ttgcgaatgt aaggatttcc aaaatcaggg agtctgtaaa
     9901 cactgggttg ccatggatct ttattttcgt agtctccccc aagctattca agaacgcatt
     9961 ggtaaagcga gtcataaacc aagttttgcc agccaactga ttcctacctt gccatctgaa
    10021 gagctccaag atgaactggt agaagaaaaa gctacggcgc cttcacttgc ccttcatgga
    10081 caagtcgaaa tccgaaatca tagcctagcc tggaccttaa aattacaggt agaacaagct
    10141 cctcgtgcct atgttatcaa ggatatagct cacttcattt ttctcatttt ccaaaaggaa
    10201 gattactttg tttcacagaa aatcgggaca atcagattat ccttgaatca gtttaatcaa
    10261 gcatcccaaa acctactcct ctatattaaa aaatatttta tcgatagaaa tgaacattct
    10321 tatttcaact tcagttatgg cattaaccct agagattacg gacgctattt agagactcct
    10381 gttagctatt taaacgacct cgtcccactt tttcaagctt tggatgtctt ccaatatgta
    10441 acctcaaaag ccgaataccc tcttatattc ttagatgata gtccctttat cccggaagag
    10501 gaaattttta aagttgtcaa atctaataac cattatgaaa tcatcaatac agcttatttt
    10561 ggtttcatta ttcaagaaaa actttggatt cgacacaatc acttccacat catcaaagaa
    10621 gaacaccgtt actttttgga taaattagct acttggatct atcattatca agaaaattct
    10681 cctcttatct tttcaaaaga aaataaggct gaactcatgc aagtctgtaa tatcatctca
    10741 aactatgttc ctatcagtat cccagatgaa ttacaaatcc atgactttat ccccaccttt
    10801 gccttctcca agactagaaa tgaaattgcc cttaatatgg tgtggtcctt tggagaaaaa
    10861 caagtccatt ctaaacaaga tctcctaacc ctcccctata cttaccaagc tagcaaagct
    10921 agaaaaatct atcatcaact gctttctgct ggtttcaaag aggaatttca tagtctttcc
    10981 aaaatcaaaa tcgttgattt cttcctaaaa gaactccccc gttttcgtac tctgggtcaa
    11041 gttcaactag atgaatcgct tgaaaaactc ttagtagaag atcctgctgt cattgacatt
    11101 tttgacgacg aaagcttcct ttcagttcag tttgattttt ctatgatttc agaagatgag
    11161 gttgaaaaag ctatccaagc tttatggaat caagaaagcc actaccaaac caagcaagga
    11221 aaggttctag tctttgacga tgaaagtctc aaggttgctc aaagtctcca agatcttcgc
    11281 gctaaatttt cagacggaaa aatcaagatg cacaaatctc gtgcctttag cctttctgaa
    11341 accttcaaag acaatgaaca tgtcaatttt tctcgtgatt tcaaaaagat ggcttatgac
    11401 ctgacccacc ctgaagaatt tgatatcaag ccttatgaag tcaaggccaa actccgctcc
    11461 tatcaaaaag aaggagtcaa gtggctgagt atgctggatc attatcattt tgggggaatc
    11521 ctggcagacg acatgggtct agggaaaacc ttgcaaacca tcactcttct ggaagccaac
    11581 cttaaaccag accagaaagc tcttatccta gctccagcca gccttcttta taattggaag
    11641 gaagaattcc gaaaatttgt gcctcacaaa caggttgaag tagcctatgg tagcaaaact
    11701 gagcgtatca aacaaattga aaaatctgct actatcacta ttacaagcta tccatctttt
    11761 cgatctgacc ttgaacatta tcaaaagcag tcttacgact acctcatcct agatgaagca
    11821 cagatgataa aaaatagtca gaccaaaaca gcacaagctt taagagaatt tgatgtcaaa
    11881 acctgctatg ccctatctgg aactcctatt gaaaaccgtc tcgaagagat ttggtctatt
    11941 ttccagatag tattaccagg tctcctacca agtaaaaagg aattttcaaa attgtcacct
    12001 caactcgttg ctaaactgat tcaacctttt gttctgcgta ggaaaaaaga cgaagttctg
    12061 acagaattgc ctgaactgag cgagcacctc tacagcaatg aactcagcag ttcgcaaaaa
    12121 acgctctatc tagctcaact tcgacgcatg caagaaatgg tttcaggagc cagcgcctat
    12181 gagattaaac gtcataaaat tgaaatctta gcaggactga cccgacttag acaaatctgt
    12241 aatacccctg ctctcttttt agaagactac aaaggggata gtggaaaaat ggacagctta
    12301 ttcgagctcc tagacaccat tcgtgaaaaa gggtcacgac cacttatctt ttcacaattc
    12361 acctctatgc ttgatctcat tgagcaagaa ctagagaaaa aagaaatgtc tcactttaaa
    12421 ataacaggac aaacaccttc agataaacgt caagaaatgg tcaatctttt caatcaaggg
    12481 gaaaaagact gcttcctcat ttccctaaaa gcaggtggaa caggactgaa tctaacagga
    12541 gctgataccg tcatcctctg tgacctctgg tggaatccag cagttgagat gcaagccatc
    12601 ggccgaagcc accgccttgg ccagaccaaa caagtcgatg tctatcgcct tatcacactt
    12661 ggtaccatag aggaaaaaat acaagaactc caagaaagta aaaaggaact cttcaacacc
    12721 gtccttgaag gacaagaaag cagaagcaat ctgagcgttg atgatattaa agaaatcttg
    12781 ggtgttgaat aaacgaaaaa gaactagagt aaccacactt agtggattta tctagttctt
    12841 ttttaggttc tagactttcc ttgccatcct atgagctcta tacgccttac catcacgttt
    12901 ctgttcgttt agatacaatt tcgccacccc tttatctgac agtttttata ctatcaaact
    12961 agggagctga ccacaagttg ctcaaagtac cgctttgaga ttgtggatag aacggacctg
    13021 gtttgaagat attttcgaag agaattattt cttgcatcag acttcaaata gtctaactac
    13081 agatatttga atctattacc tcaatccaag caggcttgag aatcactctt gggttcaccg
    13141 atagataata gacacgtagg ccttgcacat cagatctacg atagatccat cgtataaaaa
    13201 actgcaatcc acagattgca gtctttcttt attttctagc ttgatagcga ctagtcagga
    13261 tgaaaatcac tgtaaagacc aacatcatga caccataaac aggtaatgtt tgtcctgtta
    13321 aggttgcaat ttcaagcagt ttttgaattc ttgggaagag agctgtggct aggaagcctc
    13381 ctactgacca gacaatcaat aggactcgcc atagagtgaa tggcatgcag gctctaaata
    13441 cggataagaa accaattgac cccaagagat aatagaggag agttgagatt tctaactcag
    13501 accaaccttg actcgttcca aagattttca caaaaagaac gccaaacacg accatgaggg
    13561 cacttggtag ggcacgaagc atggatcttc tgaggaagtt tggctcgaca ggtttgatat
    13621 ttcgctcaaa agtcagaacg aatggtggga aaccttccac aaactggtca atcatggtaa
    13681 tctggatcgg aatgaaggga aaaatcaaaa tccactctga ccgacctagt aaagcactgg
    13741 caatacagat gactgctaac aggaaggaat agatggtctt tatcaagaaa atcggggcga
    13801 tgtgggcaat gttattgacc acgcgacgac cctcgaagag aatctcagga acatcattaa
    13861 agtctgagtt caagagaacc agattggcaa tctgacgggt tgctggatcc ccctccgcca
    13921 tcacgataga acaatccgcc tcacgaaggg ccaagatatc attaaccccg tcccctgtca
    13981 tagccgttgt atgacccgct tttttcaacg tttggatgat gagtttcttt tgatgagggg
    14041 aaacacgtcc gaaaatagct gtctcctccg ccatggccat caattcctca tcggtgattt
    14101 ttgagcaatc tacatagctg tgatagtccg caaaaccagc cttctgggca atgctggaca
    14161 ccgtaactgg attgtcacca gagataatct tgagtcccac ctcctgagaa cggagatagt
    14221 ccagcgtctc tgctgctccc tctcgaatgg ggtccaagat ttccagcaag gctagagcct
    14281 gaatatcaga tggtttctgt ggtttgtgat ggtctaattt ctcctgactg agagctaaga
    14341 ccaagacacg tgatcctctc tccaaggcct ccctggcttc tgggacttca gaatccagca
    14401 acatctcagg tgcccctaag aaaactgtcc ccaagccttc taactccata gccccccact
    14461 tgcggtcgct cgagaaggga agattggaaa tcataggata agcaacatct cccacaaaac
    14521 gctggcgaat ggcttgggca gttggattct tatcctcact atgggccatg tagctagtca
    14581 agatgctggc aatagcctct tcaccgtaag tttccgtcag tggaagaaca gcctccacct
    14641 gcatctttcc ttgggtgatg gtgcccgtct tgtccagaca gagcatatcc acgcgcgcca
    14701 aggtctcaac agagtacatc tcctgcacca agaccttttt caaacctagc ttaatcactg
    14761 cagtcaagag cgaagtaatg gtcaaaaggg caattccctt aggcaacatt cccaaaagag
    14821 ctgtcgacga gtttacaaca gatgacttga gaggcaggcc ttttaaaagc aaggcttcca
    14881 gcaagagagc cagaccaaag ggaatgataa tcttcccagt aaaaccagcc aacttgtcca
    14941 gcgatttcat gatacgggag ttgatgggtt taacggtctt agcctcaagc atgagtttgg
    15001 cagcatagtt gtctgcaccg acatggtgaa cttgagataa aactgaccca ctggctagga
    15061 aacttcctga caaaagtaag ccgtcaactt ccttttgcac caaatcactt tctcccgtta
    15121 acatggcttc attgacttcc gcaaagcctt ccaaaaccaa ggcatcacta ggaatctgct
    15181 ctcctgcaga caaacgaatg acatctccta gcactaattc ttcaggatta agagcaactt
    15241 cctgaccatc acggatggtt ttgacctttt ccttggtcat gagattgagc ttgtccacca
    15301 tgtgtttggc tcgtagctcg gtcacaatcc cagaaaaagc gttaaagcag ataacagcaa
    15361 agaagaccag attgctccaa gcctgcacaa aggcaagggc caaagcaatg gcaaagttca
    15421 aagcgttaaa aagggtaaag acatttcgtt taacgatttg ccaagtgctg gtactggctg
    15481 atgcggtaaa gtcattgacc aaaccctcag cctgtctttc cttgacttct ctttgggtta
    15541 atcccataat cttattttta tccataaatt ctattatatc atttttttta acagaattct
    15601 aatttcatcc caaatatgca tctagtcaag ggaaaagttt gccagccctc ctttgataag
    15661 gtataataat aacaagaaaa caatcgaagg agatcgcatg ttttatactt atttgcgtgg
    15721 attagttgta ttgctcttat ggtccatcaa tggcaatgct cactatcata atactgataa
    15781 aattcctaat caagatgaaa attatatttt agttgcgcct caccgtacct ggtgggatcc
    15841 tgtttatatg gcctttgcga ccaagccaaa acagttcatc tttatggcaa aaaaagaact
    15901 ctttaccaac cgtatctttg gttggtggat tcgtatgtgt ggcgcctttc ccatcgaccg
    15961 tgaaaatccc agcgcctcag ccatcaaata tcctatcaac gttctcaaaa aaagtgaccg
    16021 ctctctcatc atgtttccaa gtggtagccg ccactcaaac gatgtcaagg ggggcgcagc
    16081 actgattgcc aaaatggcca aggtccgtat catgccggtt acctacaccg gtcccatgac
    16141 tttgaagggc ttgattagcc gtgaacgtgt cgatatgaac tttggaaatc caatcgatat
    16201 ctcagatatc aagaaaatga atgatgaagg cattgaaaca gtcgccaatc gtattcaaac
    16261 agaattccaa cgtctggacg aagaaacgaa acaatggcac aatgataaaa aaccaaatcc
    16321 actctggtgg tttatccgca tccctgccct catccttgct attatcctcg ctatcctaac
    16381 catcatcttt agctttatcg caagcttcat ctggaaccca gataagaaaa gagaagaact
    16441 tgcatagaag aaatgaacct tggccaaaca gctaaggttt tcatttatat agtagattgg
    16501 aactagaata gtacacctct acttctaaaa catttttaga aatcgatttg actgtcctga
    16561 tcgattttgt cctattctta tttcatttta ctatatttat aagaatcccc taatcaaatc
    16621 aaaagccact ctacaataga gtaactttaa gattgtccat ttatactcaa tgaaaaagcc
    16681 ctctgaaaat cagagagctg atttgagctc aggctaaaat cctagtgaaa caaaaaaatc
    16741 tacacgatca gaaaagtctc tatcgtgcca ttttcataca tgatgtatag tccaagtaga
    16801 atgaatacta agggcacaat gattcgctcg tatttttcaa ttgtctcgaa tattaacgga
    16861 atagaggata acaccctact aatctcgcaa aagataatta tgccgattac aaacacaagc
    16921 aaggccacga gggtctgtga ccaatctaac gaagcaaaat aaggtatata gatacctaaa
    16981 ttatctccgc cagacgcaat tgtcagcaat gtaactgtcc aaaacagttg atttgccttg
    17041 ctttgttcta atctttgaat aatttcttcc tcttcttctt ccgcatcttc tccaacaatt
    17101 gcaaagcgaa tccctaaata gatagggatt aaaccaagca atccaaccat ccattcttca
    17161 ggcacgaaat taacgacata agcagcaact aaactcgccc ctacaagtaa gcctgtgcct
    17221 agatattgcc ccgcataaat atgccatttc tgtttattct gtgatagctg tgcaaataaa
    17281 ataattaaaa taattaaata atcgatactg gtggaaatat aaacaccaat agcagatatg
    17341 attgtctgtc ccataaaaaa aacctcctgt attagtcagt aataaatcag cagattttag
    17401 gagagcacaa acacattaat ttagctatcg ctagtgggta atgtcgaacg taggaaagca
    17461 ttttactcct cctcaaaacg tagttacaaa gtaatttcta actggaattc agtgactact
    17521 tccatgtaaa gtataacata ctatacttta ctttgtaaag agtgggctct tctagggggc
    17581 ttgcatacag ctactcggct tgaaaagccg agtagctgcg cacctttatg gtgtaattag
    17641 ctgataccat ttgagatttt tgtagtctcc aaaattgtta ccgataaaac ctcaataaaa
    17701 aagccctctg aagtcagaga gctgatttta gcccgggcta aaatcctagt gaaaaagatg
    17761 aaactccttg tgctcatcga acactgtgtc atttccctat tttcatacgg atttttgaca
    17821 cccttagcat catgattctt gctagataaa tcgtttatag actaggggac gaggtgaaga
    17881 tcgtacgaaa ctttcagtga aacaaaaaaa tctccacgaa gggagaaaag ctagtttatt
    17941 ttaccttaca gtgctaggaa ccgtaaccga agattatact tgagtatagc gaggtaaggt
    18001 gacaacgcaa caaagctacc caatttagag tagctttatt atcaagatat gctcaattga
    18061 acacggccta agcacttggc aaaaaagata aaatctccta gaaactgaag tttcttcgtc
    18121 aattttccta tttttgcttt gtgcttttga cggcctttgt atcttgaatt agcgacgaag
    18181 tcctagagag ttgatcaact cacggtaacg gttaacgtcg tttttacgca agtatgcaag
    18241 caagttacga cggcgaccga tttttttcat caatccacgg taagtagcgt ggtctttttt
    18301 gtgttgtttg atgtgttcgt taaggtggtt gatttcccaa gtaaggacag caacttgaac
    18361 ctctactgaa cctgtatcac cttcgtgacg tgcatattgt gcaatgattt catttttttt
    18421 ctcttttgag attgccatga tgtttctcct ttttatttgg cttcatccga gtgacaggtt
    18481 ggcatgcctg taaccaagaa gaagttattt gtctttacga caatccctat tttaccaaga
    18541 tttaaatttt ctgtcaaaca ttaatactct catagttatt atttttcaag taaagagata
    18601 cgttctttta tatgatgacc tttttcaagt tcatctacca atccaatagc ataatctgca
    18661 taactaatct ggcttattcc ttttccatta gtagtaaaaa tttctcctgc tagaatgtag
    18721 tttcctgttt tttcaccatc gggaataaaa tctacagcag gactgacaaa agtccagttg
    18781 agattatttt ttgtacgaag aagatcaaat tcatccgctt gagcttttgc tagtgactta
    18841 aattcttcag gaaaatcaga cgtatctaaa agtcgtgtcg tttttgtttc atctatataa
    18901 agacttcctg ctccccctac aactagaaaa cgtgtctgtg ttccagcaag aagctgatta
    18961 aatagttcga ttgatttgct gtggagcggt agcgtatctg gtgtataagc accaaacgct
    19021 gaaataacag catcaaatcc agtaagatca tcttttgtca actcaaataa atctttttta
    19081 ataatagact cagcttgact tttgttttca gaacgaacaa tagccgttac ttcatgtcct
    19141 cgtttgactg cttcttcaac aattgctttc cccgcttgtc catttgctgc aataactgct
    19201 agtttcattt tttatacctc tattgttgta attattttag ttacaacaaa tgtgacactc
    19261 ttaataatca atatcaatag tcttgcttaa ttattatcaa aatatttcta ccaagaaaac
    19321 taactatgat tctagtgaaa agaaaaatct tctttgtcaa caaatttact ttcttgtttt
    19381 aaacatgcta taataatcat agcaagagat ctaagttgtc tgttttttta aaacgaggtg
    19441 attatcatgc gtagattcta ttcccatctc ccctactatc tggtcatatt attcttttat
    19501 tggccacttt atgagttgtt cttgctagtt gtttctgacc cctttacact atataaacaa
    19561 tcttctcttc tttacacctc tggtaatctt gattgtatcg ttactctata gctaccgttt
    19621 ccgtttctca ctttgatggt tagttggtaa cggactgctc ttttacttta ctatcataac
    19681 ctttggtgag tttatactaa tttacttgct aatctatgaa acagttgctc tggtgggcat
    19741 ggattctggt attagcatca agcatattct acaaaatatg aaaaacaaaa aactttcaca
    19801 aaatccttga aaaatctcac aatcatgcta taataatcca tagagacaag tcacttagtc
    19861 cctttctact agagagtgcg tggttgctgg aaacgcatag gaagtctaaa ctgatactac
    19921 tcttgagttt tttatgaaaa cataaaacgg tggccacgtt agagccgatc agaggtgtcc
    19981 ctctcttttg aggtacataa atgaaggtgg aaccacgttg cgacgtcctt tcgaggatgt
    20041 cgcatttttt tattaggata ctaattatgg agttgcaaga attagtggag cgcagttggg
    20101 caatccgaca agcttatcac gaactggaag ttaagcatca tgatttcaag tggacggtag
    20161 aagaagacct cttggcttta tctaatgata ttggaaattt ccaacgactg gtgatgacaa
    20221 agcaaggacg ctactatgat gaaacaccct acacactgga acaaaaactt tcagaaaata
    20281 tctggtggct attagaactt tctcaacgtt tggatataga cattctgacg gaaatggaaa
    20341 acttcctctc tgataaagaa aagcaattga acgttaggac ttggaagtag tctgctgata
    20401 aaaaatcaat gcttagaaac tatgaaataa taaaaaagga gaacatcatg attaacatta
    20461 ctttcccaga tggcgctgtt cgtgaattcg aatctggcgt aacaactttt gaaattgccc
    20521 aatctatcag caattcccta gctaaaaaag ccttggctgg taaattcaac ggcaaactca
    20581 tcgacactac tcgcgctatc actgaagatg gaagcatcga aattgtgaca cctgatcacg
    20641 aagatgccct tccaatcttg cgtcactcag cagctcactt gttcgcccaa gcagctcgtc
    20701 gtcttttccc agacattcac ttgggagttg gtccagccat cgaagatggt ttctactacg
    20761 atactgacaa cacagctggt caaatctcta acgaagacct tcctcgtatc gaagaagaaa
    20821 tgcaaaaaat cgtcaaagaa aacttcccat ctattcgtga agaagtgact aaagacgagg
    20881 cacgtgaaat cttcaaaaat gacccttaca agttggaatt gattgaagaa cactcagaag
    20941 acgaaggcgg tttgactatc tatcgtcagg gtgaatatgt agacctctgc cgtggacctc
    21001 acgttccatc aacaggtcgt atccaaatct tccaccttct ccatgtagct ggtgcgtact
    21061 ggcgtggaaa cagcgacaac gctatgatgc aacgtatcta cggtacagct tggtttgaca
    21121 agaaagactt gaaaaactac cttcaaatgc gtgaagaagc taaggaacgt gaccaccgta
    21181 aacttggtaa agagcttgac ctctttatga tttcacaaga agtgggacaa ggtttgccat
    21241 tctggttgcc aaatggtgcg actatccgtc gtgaattgga acgctacatc gtaaacaaag
    21301 agttggcttc tggctaccaa cacgtctaca ctccaccact tgcttctgtt gagctttaca
    21361 agacttctgg tcactgggat cattaccaag aagacatgtt cccaaccatg gacatgggtg
    21421 acggggaaga atttgtcctt cgtccaatga actgtccgca ccacatccaa gttttcaaac
    21481 accatgttca ctcttaccgt gaattgccaa tccgtatcgc tgaaatcggt atgatgcacc
    21541 gttacgaaaa atctggtgcc ctcactggcc ttcaacgtgt acgtgaaatg tcactcaacg
    21601 acggtcacct attcgttact ccagaacaaa tccaagaaga attccaacgt gcccttcagt
    21661 tgattatcga tgtttatgaa gacttcaact tgactgacta ccgcttccgc ctctctcttc
    21721 gtgaccctca agatactcat aagtactttg ataacgatga gatgtgggaa aatgcccaaa
    21781 ccatgcttcg tgcagctctt gatgaaatgg gcgtggacta ctttgaagcc gaaggtgaag
    21841 cagccttcta cggaccaaaa ttggatatcc agattaaaac tgcccttgga aaagaagaaa
    21901 ccctttctac tatccaactt gatttcttgt tgccagaacg cttcgacctc aaatacatcg
    21961 gagctgatgg cgaagatcac cgtccagtca tgatccaccg tggggttatc tcaactatgg
    22021 aacgcttcac agctatcttg attgagaact acaagggggc cttcccaaca tggctggcac
    22081 cacaccaagt aaccctcatc ccagtatcta acgaaaaaca cgtggactac gcttgggaag
    22141 tggccaagaa actccgtgac cgcggtgtcc gtgcagacgt agatgagcgc aatgaaaaaa
    22201 tgcagttcaa gattcgtgct tcacaaacca gcaagattcc ttaccaatta attgttggag
    22261 acaaagaaat ggaagacgaa acagtcaacg ttcgtcgcta cggccaaaaa gaaacacaaa
    22321 ctgtctcagt tgataatttt gttcaagcta tcctagctga tatcgccaac aaatcacgcg
    22381 ttgagaaata agagtctagc ataaaagcct ccaatctgga ggctttttct catctatttt
    22441 tactcaagga ctaagttcac ttgagcaaac tgaatccgca ctgtcgttcc ttttccgacc
    22501 tcagactcga tacgaatctg gtgccccagt tcttcagaaa ttttcttaga tagataaagg
    22561 ccaagtccag aggactgctg ggtcaaacgg ccattgtatc ctgaaaagcc acgttcaaat
    22621 actcggagga catcactgtt ttttatcccg attcccgtat ctttgataca aagctcttgg
    22681 tcatccatat aaatctccag accaccttcc ttggtgtact tgagactgtt tgagatgatt
    22741 tgctcaataa ccactagcag ccacttttta tccgtcacga tttctttatc aaggtcatgt
    22801 agattgacat ttaagccttt ttgaataaag aaaagagcat atttacgaat tatttccttg
    22861 accaagtcct caatttgaac ctgctttaag accaaatcat catggaaact ttctaaacgc
    22921 aggtactgta aaactaggtt ggtataggag tcgattttga aaatttcctg ttctagttgc
    22981 tgcttcagtt ggcggtcgac cacttctgca actaagagtt gactggctgc aatgggggtc
    23041 tttatctgat ggacccacaa ggtatagtaa tccagcaaat ccgtcagttt tctttctgct
    23101 tttgacctct gctgatagag ttccatctca cgcgcttcta atttttctgc taaagctatt
    23161 tccaaaggag acttggcttc cctctctcca tagagaagtt cctggcgata gacctgcgtt
    23221 tccaccaata tgtcccaagt gaaaaataat atggttacaa agcaacacaa gaagaaaaag
    23281 tagaggaagt aaattcctag actggcaaat aaaaactgaa agagtaagac aagaaatgcc
    23341 aaagaaagca gatagataaa aagacgacta cgggagcgca gataggctag aaaaaattgt
    23401 ttccaatcaa gcatgcttca atccgtaccc tattcctttc ttggtctcga taaatcctac
    23461 caatccctgc tcctccaact ttttacgcaa acgagccaca ttgacagaga gggtattatc
    23521 atcaatgaaa aagtcactgt tccaaagttc ccgcatcagg tcgtcacgtg ctacgatgtt
    23581 gcctgcatgc tcaaataaca cgcgtaaaat ctggaattca ttcttggtca aattcaagac
    23641 ttgcccttga taatgtaaat ccatggattt ggtattgagg ataacaccag catattccag
    23701 caaactctca tcacgcccaa actcatagga acgacgcaac aagccctgaa ccttagctaa
    23761 aagaacctgc tggtcaaaag gcttggtcac aaagtcatcc gcccccatat tgattgccat
    23821 gacaatatcc atagcctggt ctctcgaaga aagaaacatg ataggtacct tggaaatctt
    23881 gcggatttcc tgacaccagt gataaccatt aaacaagggc aaaccaatat ccatgaggac
    23941 cagatgaggt tccgactgaa caaatagact caaaacttcc ataaagtctt ctaccaggac
    24001 cacttcaaat ccccattcag agagcatttt cccaatctgt tgacgaatga cctgatcatc
    24061 ttctattaat aaaatcttgt gcatgcgctt ctccttttcc attattataa cagatttttc
    24121 catgctagat ggtctgaaac tgaatttgaa atagcctgtt tttagccagt acaaacaggc
    24181 tatgctacta gctaatttga gggaaatttg ctaagataaa taaaaagaaa ggagctctta
    24241 tggccaatat ttttgactat ctgaaagatg tcgcatatga ttcttattac gaccttccct
    24301 tgaatgagtt agacattcta accttaatag aaatcaccta cctctccttt gataatctgg
    24361 tctccacact tcctcaacgt cttttagatc tagcacctca ggttccaaga gagcccacca
    24421 tgcttactag caaaaatcgc cttcaattat tagatgaatt ggctcaacac aagcgcttca
    24481 aaaattgcaa actctcccat tttatcaacg acatcgaccc tgaactgcaa aagcaatttg
    24541 cggctatgac ttatcgtgtc agcctcgata cctatctgat tgtctttcgt gggacagatg
    24601 acagtatcat tggctggaag gaagatttcc acctgaccta tatgaaggaa attcctgctc
    24661 aaaagcacgc ccttcgctat ttaaagaact tttttgccca tcatcctaag caaaaggtta
    24721 ttctggctgg gcattccaag ggaggaaatc tcgctatcta tgctgctagc caaattgagc
    24781 aaagtttgca aaatcagatc acagcagttt atacatttga tgcacctggt ctccatcaag
    24841 aattgacaca gactgcgggt tatcaaagga taatggatag aagcaagata ttcattccac
    24901 aaggttccat tatcggtatg atgctggaaa ttcctgctca ccaaatcatc gttcagagta
    24961 ctgccctggg tggcatcgcc cagcacgata cctttagttg gcagattgag gacaagcact
    25021 tcgttcaact ggataagacc aacagtgata gccggcaagt agacacaacc tttaaagaat
    25081 gggtggccac agtccctgac gaagaacttc agctctactt cgacctcttc tttggcacta
    25141 ttcttgatgc tggtattagc tctatcaatg acttggcttc cttaaaggcg cttgaataca
    25201 ttcatcatct ctttgtccaa gctcaatccc tcactccaga agaaagagaa accttgggtc
    25261 gccttaccca gttattgatt gatactcgtt accatgcatg gaaaaataga taatactctt
    25321 gaaaattaaa tgtatacaaa acaaaagacc tagaatacat actttcatgt tcattctaag
    25381 tctttttaaa tagaatctaa tagtcaataa aaatcaaaga gcattgagag ataatggggc
    25441 ttggaacgtc cctctcgttt caacaaaatg accccattat agattaaaaa gatgccactt
    25501 agaaaaagca aaaaaggaag taagacaaag gcaaatacat aaaaagctaa ctgaacattc
    25561 tcgtatccat ttttataaaa aaggtaggat agataaaaat aacttgaaat gagggataat
    25621 aaaaataata ctggattcca caaacttcta ttatccttcc aaaatgacac tataaaggct
    25681 aatacaattt ctataacgag atacatttct tactccttta atagctacat tttatcataa
    25741 ttatccaaag aaaaaaagag ggcatttatc cctcttaatc cttcatctga ctctctgcat
    25801 cggccacgac tttttctaga ctggtctgac caagttctgc ctccatagtc aactgaattc
    25861 tctccaattt ttgatccaaa acatcatgaa tatgagctcc tacagggcaa tttggattcg
    25921 gattgtcatg gaaactgaag agttgacctg tcttaccaag acattcgacc gcctgataaa
    25981 catctaaaag actaatatcc ttaaggtcct tgacaatctc tgttccgccc gttccacgcg
    26041 ctactgaaat cagctctgcc ttcttcaact gggacaagat ctttctgata atgacaggat
    26101 tgaccccaac actagcagcc agaaaatcac tggtcacctt gctttccttc ccctcgaggg
    26161 caatgattat cagcatatga gttgcaatgg taaatctact tggaatttgc atcctcttct
    26221 cctttttacg aggctaccct gcctctactc ttctttttct attattatac cctttttagt
    26281 tgtaatgtca atcgttacca cttttcaacc agtcgtctaa ctcccgatcg caaccctctt
    26341 tctgagccaa ttctctcaaa aattcctgat gatgagtatg gtggatccca ttgaccagac
    26401 tttcatagta aacctcaaaa tagggaagtc tcaggtcttt agccagctgc aattcagctg
    26461 ccacatcgta atctacccgt cggaagtcca tatctaccag gcccttgtca tcaaactcca
    26521 aaatcatata ctgggcccgc aagtccttcc gtagctgagc gtccaaaaag aaaggttggc
    26581 caatcgaacc cggattgaca atcaattgcc caccagtccc gtaacgaagc aactgctggt
    26641 gaatgtgtcc ataaacagca atatcacagg gaggatgagt caccaagcgg tcaaactcct
    26701 cttgtttgcc agtatgaatc aactctcgcc cccagttctt atcaggcaga tgatggctaa
    26761 ttcccactgt caaatcccca aactgacgat gaatttgaag aggttgattg tggagcactt
    26821 caatttcttc tagggaaatt tcctctaaaa catactggca ctggcgcaag agatagcgtt
    26881 gactagggcg agtactatcc aattccttac ggacaccatg ccaaagactg tcttcccagt
    26941 ttcccaaaac tctagccgta atcggtagtt gatccaacaa gtccaaaatc cttctacgcc
    27001 ctgtccctgg catgagaata tctcccaaaa gccagtattc atccactcct agctgccgag
    27061 catctgccaa aacagcctcc aaggcggtgg tatttccatg aatatctgaa agaagagcta
    27121 ttttcgtcat atccatctcc tcgttttttc tcttgcaata agtataacat aaaaagtcac
    27181 agctagagaa atctagcttt ttttgatata ctagataaag atattagaca agaggaaacg
    27241 aatgacccca aacaaagaag actatctaaa atgtatttat gaaattggca tagacctgca
    27301 taagattacc aacaaggaaa ttgcggctcg catgcaagtc tctccccctg ccgtaactga
    27361 aatgatcaaa cgaatgaaaa gtgaaaatct catcctaaag gacaaggaat gtggctatct
    27421 actgactgac ctcggtctca aactggtctc tgagctctat cgtaagcacc gcttgattga
    27481 agtttttcta gttcatcatt tagactatac aagtgaccag attcacgagg aagctgaggt
    27541 cttggaacac actgtctctg acctgttcgt ggaaagacta gataaactgc taggtttccc
    27601 taaaacctgc ccccacggag gaactattcc tgccaaggga gaactcctcg ttgaaatcaa
    27661 taacctccca ctagctgata tcaaggaagc tggcgcctac cgcctgactc ggatgcacga
    27721 tagttttgac attctccatt atctggacaa gcactcactt cacatcggtg accagctcca
    27781 agttaagcag tttgatggct tcagcaatac cttcactatc ctcagtaacg acgaggattt
    27841 acaagtgaat atggacattg caaaacaact ctatgtcgag aaaatcaact aatttctcaa
    27901 gtcccctacc aaccctgaaa gtttt
//
LOCUS       DAVKQB010000029        25804 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKQB010000029 DAVKQB010000000
VERSION     DAVKQB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.029"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            119..283
                     /locus_tag="VW528_001244"
     CDS             119..283
                     /locus_tag="VW528_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9912064.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            367..933
                     /gene="amaP"
                     /locus_tag="VW528_001245"
     CDS             367..933
                     /gene="amaP"
                     /locus_tag="VW528_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9912065.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            945..1115
                     /locus_tag="VW528_001246"
     CDS             945..1115
                     /locus_tag="VW528_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9912066.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1154..1762
                     /locus_tag="VW528_001247"
     CDS             1154..1762
                     /locus_tag="VW528_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9912067.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1793..1996
                     /locus_tag="VW528_001248"
     CDS             1793..1996
                     /locus_tag="VW528_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9912068.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2091..2369)
                     /locus_tag="VW528_001249"
                     /pseudo
     CDS             complement(2091..2369)
                     /locus_tag="VW528_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2567..2854
                     /locus_tag="VW528_001250"
     CDS             2567..2854
                     /locus_tag="VW528_001250"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9912069.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3132..3902)
                     /locus_tag="VW528_001251"
     CDS             complement(3132..3902)
                     /locus_tag="VW528_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU9912070.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3917..4765)
                     /locus_tag="VW528_001252"
     CDS             complement(3917..4765)
                     /locus_tag="VW528_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU9912071.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4762..6237)
                     /locus_tag="VW528_001253"
     CDS             complement(4762..6237)
                     /locus_tag="VW528_001253"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU9912072.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6560..7915)
                     /locus_tag="VW528_001254"
     CDS             complement(6560..7915)
                     /locus_tag="VW528_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9912073.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGELIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7925..8242)
                     /locus_tag="VW528_001255"
     CDS             complement(7925..8242)
                     /locus_tag="VW528_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9912074.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8259..8576)
                     /locus_tag="VW528_001256"
     CDS             complement(8259..8576)
                     /locus_tag="VW528_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9912075.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8589..10274)
                     /locus_tag="VW528_001257"
     CDS             complement(8589..10274)
                     /locus_tag="VW528_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEU9912076.1"
                     /translation="MKNKKLILSLFILFLLIFIHAKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            complement(10279..11175)
                     /locus_tag="VW528_001258"
     CDS             complement(10279..11175)
                     /locus_tag="VW528_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU9912077.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(11178..12440)
                     /locus_tag="VW528_001259"
     CDS             complement(11178..12440)
                     /locus_tag="VW528_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9912078.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            12720..13898
                     /locus_tag="VW528_001260"
     CDS             12720..13898
                     /locus_tag="VW528_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9912079.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(14126..14572)
                     /locus_tag="VW528_001261"
     CDS             complement(14126..14572)
                     /locus_tag="VW528_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9912080.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVQKYSRLKLE"
     gene            14642..15301
                     /locus_tag="VW528_001262"
     CDS             14642..15301
                     /locus_tag="VW528_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9912081.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACGIIFGKLLL
                     V"
     gene            complement(15353..16504)
                     /locus_tag="VW528_001263"
     CDS             complement(15353..16504)
                     /locus_tag="VW528_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9912082.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGNRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(16519..17250)
                     /locus_tag="VW528_001264"
     CDS             complement(16519..17250)
                     /locus_tag="VW528_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912083.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            17479..17580
                     /locus_tag="VW528_001265"
                     /pseudo
     CDS             17479..17580
                     /locus_tag="VW528_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(17552..18328)
                     /locus_tag="VW528_001266"
     CDS             complement(17552..18328)
                     /locus_tag="VW528_001266"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9912084.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLGETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(18321..19544)
                     /gene="trpB"
                     /locus_tag="VW528_001267"
     CDS             complement(18321..19544)
                     /gene="trpB"
                     /locus_tag="VW528_001267"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9912085.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGCPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(19522..20121)
                     /locus_tag="VW528_001268"
     CDS             complement(19522..20121)
                     /locus_tag="VW528_001268"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9912086.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     YFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            complement(20108..20875)
                     /gene="trpC"
                     /locus_tag="VW528_001269"
     CDS             complement(20108..20875)
                     /gene="trpC"
                     /locus_tag="VW528_001269"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9912087.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(20872..21876)
                     /gene="trpD"
                     /locus_tag="VW528_001270"
     CDS             complement(20872..21876)
                     /gene="trpD"
                     /locus_tag="VW528_001270"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9912088.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIITGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(21887..22453)
                     /locus_tag="VW528_001271"
     CDS             complement(21887..22453)
                     /locus_tag="VW528_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9912089.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQTIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            complement(22450..23811)
                     /gene="trpE"
                     /locus_tag="VW528_001272"
     CDS             complement(22450..23811)
                     /gene="trpE"
                     /locus_tag="VW528_001272"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9912090.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEELEKALNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(24732..25733)
                     /locus_tag="VW528_001273"
     CDS             complement(24732..25733)
                     /locus_tag="VW528_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9912091.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT         8111 a         5499 c         4131 g         8063 t
ORIGIN      
        1 caaagaaatt gattttttga tattaaaata aaaaaggagg gtaggcagtg ttgtgatcaa
       61 ttattgctgg aggtcttatt ggtctcttgg caggtaaaat cactaaaaaa gtagttctat
      121 gggaatcatc gcaaatgtat tcgctggttt agtcggggca tatgcaggac aatctctttt
      181 aggtagttgg ggtccatcaa tcgctggaat ggctttgctc ccatctattg taggtgcagc
      241 gattgtgatt actgtagtgt cattctttac aggtagaaag taaacttttc gccagtaaag
      301 ttagcaaact atttttaaat caatgacggg aaaaatagtt taaatgttaa atcgaaagga
      361 ttgtatatgt caaaagcaaa gaaaatatgt ttcattattt tctgtatttt aatcttgaca
      421 attttccttc ctgttttgat agattatcat caagttagtg atctaggtat tcatctactt
      481 agctggagac agaactccgt agttgaattc tatcttgcta gatatgtctt ttgggggaca
      541 gtggttctat caactttagt tttattatcc attttagttg tgatgtttta tcctaaacgt
      601 tacttggaaa tccaacttga aactaaaaac gatacattaa aattaaagaa ttcggcaatc
      661 gaaggttttg ttagaagttt ggtgagtgat catagattga tcaagaaccc aactgttcat
      721 gtaaatttac gaaaaaataa atgtttcgtt catgtagaag gtaaaattct tccttcagac
      781 aacatcgctg acagatgcca aataattcaa aatgaaataa ctaatggatt gaagcagttt
      841 tttggtattg agcgtcaagt aaaacttgaa gttgcagtaa aaaattacca accaaaacct
      901 caaaacaaaa agactgttag tcgtgtgaag taaggaagta aaaaatggaa tggcttaaac
      961 aatatcgata tccaattatc gctggtctca taggcgtatt tctggcttgt ttgattgtct
     1021 cctttggctt cttcaaaaca atatttgtat tgattttagg agcactggga gttgcagctg
     1081 gattatatat cgaaaaaaac tatatagata aataaaaaaa taaaaattac taatttaatt
     1141 aaaggagttt catatgtcaa acgaaaaaaa cacaaacact aacgtagaaa agaaagatgc
     1201 tactgttgta gctcacgaaa tcaaagggga acttacttac gaagataaag ttatccaaaa
     1261 aatcattggt ctttcactag aaaacgtttc aggtcttttg ggaatcgatg gtggtttctt
     1321 ctcaaatctt aaagaaaaaa tcgttaacag cgatgacgta acaagtggtg ttaacgtaga
     1381 agttggtaaa acacaagttg cagttgactt aaacgttatt gttgagtacc aaaaaaatgt
     1441 tccagcttta tattcagaaa tcagagaaat cgtatcttca gaagttgcta aaatgactga
     1501 cttggaaatt gttgaaatca acgtaaacgt tgtcgacatc aaaactaaag aacagcatga
     1561 agcagactca gtaagccttc aagatcgcgt atctgacgtt gctgaatcaa caggagaatt
     1621 cacttcagaa caattcgaaa aagctaaatc tggtcttgga tctggtttct caactgttca
     1681 agaaaaagtt agcgaaggtg tagaagctgt taaaggtgca gcaaatggtg tagtatctca
     1741 cgaaaacact cgtgtaaact aagataaaat aaatataaca ggagaaatta tcatgtcagt
     1801 agaagaaaaa ttaaatcaag ctaaaggttc tattaaagaa ggtgttggga aagccatcgg
     1861 tgatgaaaaa atggaaaaag aaggagcagc tgaaaaagtt gtttctaaag taaaagaagt
     1921 tgccgaagac gctaaagacg ctgtagaagg tgctgtagaa ggtgttaaaa acatgttgag
     1981 tggcgacgat aaataaggtt aaaagttact ttatcttttt ggtaacatta gtcaagatga
     2041 ttctcagaaa acaaaaagct agagattccc aattgcggaa ccctagcttt ttaattttgc
     2101 ctctttctct tattatattt cagcaggttg ttggccatga gtacgaatcc catgtcaatt
     2161 ctcacttgac gcttccctct cagatgacat ctcttataac ccaaacaaac ctttatctgc
     2221 ccaaagacag atttcatatc aatcttacgt ttagcgaaaa tttgtctacc cttggaagat
     2281 aaaagtgcct gatattcttt agtttttaaa cactggtaac gttcattcat atacagtccc
     2341 ttttgagggg ctgattcagg ttcataatcg cagtcaacat tgatttcaag gctgtttgct
     2401 ttctatctcc cctttttcat gatatatttt cgtacaggtg cttcaatagt ttctatagta
     2461 aaatgaacca aaaatagtac acgttgcggt ataatgttct tgtggcatat tcattagatt
     2521 ttcgtaaaaa gttctctcct atttgtgagc gaacaggtat agtcaattga ttgtcagttt
     2581 gaagaccaag agtcgtaagg caaaggatat ggcagaatcc atccaagggt ggctggcaca
     2641 atttctagtg aacctcttta aatctattac gtttgactgt ggaaaagagt tttcaaaatg
     2701 gaaagatatt tccaatcatc acgatagtga gagctttttt gcgaatctag gatgtccaag
     2761 acagcggtgt ctcaacgaac attcaaatag acttttaagg tgtcatgact tgccaaagca
     2821 gacagatttc aacgaagtca gtcaagaatt ttaggctggg taaaaactca atttcaggag
     2881 aaaatgaaat aaatattccc acaataaaat gcatagtatc aaagtttttc caacacctga
     2941 tattatgcat tttttaattt taaagacttt ttcccagcct caagaaatca catcccacct
     3001 aaatcctttg gataccaaat acctcatcag gtattttctg agttggtaag actgtctagc
     3061 ttaatttgac aaatggaggg cgttaaatcc agtttttaca ggaagtctat ttaaatattt
     3121 tcacactgac cttacaaaat atgaaacaaa ttatataaag tcattcccat gacaaagaat
     3181 aacatccctt taaaacagta tcttacccaa ttttcaggta agacttttga tacaaccgtc
     3241 cccaataccc ctccgcatat agaagcaaga aaaataactg gaacaggtgc taaatgatac
     3301 tgatttaaac ctgtaaccac aacaattgta gccaaatgag acagctggga aaagaaaata
     3361 attgcaagag aatacatcgt tgcttctttt atcgaaatag aaaaaaacac catcaataaa
     3421 gatacattga gcggaccacc accaatccct aaaaaactag aaattaagcc caatagaaga
     3481 ccacaaatca tatagaatcc tatcttatca aattgataag tttttttaaa atctgttaaa
     3541 acaaatgata caagtagcat cacaaatagt aaaatcattt gaacaatgga tactagccgt
     3601 actgataatt gagataacaa aacttgaaaa ataagatgac ctagaaatcc tcctaaaaca
     3661 gaagaaaagg ataccgtaaa aatcaatctc cattttatct ctttactttg tgaaaatctt
     3721 ttgattgttg aaataatcgc cattataaaa acagagaaac tagaataaaa agcaatatca
     3781 ctgaccgaat ggtatccaaa actatccata agtggcttaa ttataattcc accacccatg
     3841 cctgatatag aacccaatcc actagctatt ataatgatta gaaaataaat aatctcaacc
     3901 actttttaag tccatcctat tctttcaaac atctaaaacc tagatggcta ctagtggaag
     3961 tagaaataca tccatttcta gcagctactc ggtatcgatt acaataagaa caatgacata
     4021 aaaaggaacc tcctctgata gcatactcct catcttgtat cccatatttt ggcaactcat
     4081 agtcatcttc attaaaacta gctaataagg tatatctcgg attgcgacac cattcccaaa
     4141 cattcccaat catttgatat agaccaaaat cattaggtaa aaattcatag acaggagccg
     4201 ttcctataaa tccatctaag gcagtatttt cataaggaaa tcttccttgc caagtattgg
     4261 catgataata tccaccttct aaaagagtat ctccccaagg atattcagat tgtcttcctc
     4321 ctctagcagc atattcccac tgtgcttcag tcggtaaaga cattccgctc caattacaga
     4381 aagccaatgc atcttctaaa gcaacatgaa ctactggatg gtcttctaaa cccactagat
     4441 tactattctc accgtaggga tgtttccagc aagcgttcgg aacttgtaac caccacggag
     4501 ccccagcagg atgaggatat ccctcccttt ctgcctctgg aacgaacaat ataaaaacaa
     4561 aagaccactc ttgtcgctca gctagtgttt tatagcctgt ttctttcaca aattgagcaa
     4621 attcttgatt cgtaactgga gtatccgcaa tggaaaaatc ctttacacta acagcgactt
     4681 gaggaccttc aaaatcatct aaaaagccct cttcagaatt tgttcccatc ataaatgtcc
     4741 cacccttgat ttttttcatt ttcatgattg actatccccc gttttcgtta gagttggcgt
     4801 tattttagaa agttcaacag gaactaactc ttcatccaca acaaaacctt cttctctata
     4861 tctaagaaag tctactaatt ttttcttcat ctgacgaaca ataggttgat atttttcact
     4921 tggatacaaa tcattcattt catgcggatc ttttttcata tcaaataact gataatgatt
     4981 taaaacagga aaccagataa atttccactg gtctgtcaaa atatattgac tagaatcttt
     5041 tcctaaagca tgttcaccat gaaattctgt tcgccacccc tcgtactgtc caaataataa
     5101 attcttaaca ctccgtccat ctagttcatc tgtagtagtt cctcctgcca aatccacgag
     5161 tgaaggaaaa atatcttgaa ttttcactaa ttgcttgatt gttcccctat taccagctat
     5221 caatccagcc ggatcgtaaa taaacgaagg aatgtggata cttccctgat aaggatatcc
     5281 ctttctaaac agataatgtt caccaagctg atctccatga tccgaaacaa accagataat
     5341 cgtatcttta tcatgacgaa attcttttag ggcagtcaaa aagcgactaa tttgatggtc
     5401 tatatgagta atcaatccaa aataagcagc aaccattctt ctttgatcat cttcctttaa
     5461 tttccctctt aaagcataaa tagacggaat ttgcttttct aatacttccc aattcccgat
     5521 atgcaaatcg agaaattgag gcaagcgttc catatacata tcaaaatagt attttggggg
     5581 atttaatgga gcatgtggtt tttcaaaaga cattttgaga aaaaaaggaa ccgttggatc
     5641 tcttctttgt aaaaatgaaa tggactcact cacaacccag tttgttggat gtaacttctc
     5701 atctttatcc caaggtctag cttcccaaga attacaatcc attccgtcat caatcaaatc
     5761 aacatcataa ccaactttac cctttaaaaa ggctagatag tcactagcat aatcaaattg
     5821 acttccataa gccttatcat actttctatc aacatgtaga tacccatcgt gaagtaagac
     5881 atgatcaaac ccaagtctct gtctggaagg aaaaacatgc atcttaccaa tacactctgt
     5941 ttgataaccc atatctttaa agacttttgg aagagtatta gtaaaattcc aaggcacctc
     6001 atcttgataa ccaactcgtc cacttttatc ttggtctaag ccagtcaata aagctgccct
     6061 agcaggaacg caagaaggaa ctggagaata ggcattttca aaattataac ctacactagc
     6121 catcatatct aatgttggag tgctaactag cttatcctta ctattcaagg ataaggcgtc
     6181 tgctctcatt tgatctacaa caatcaaaat aatatttggc tgttttgtct gaaccataaa
     6241 atctcctttc taatatggca aaagaggcac aagaagatat ctacctttac tgcacccctt
     6301 tctatatcaa tctctctata taaaacaata acattcttgt tatgttttat agaaaaatga
     6361 actaaaatat gactaaatcg attaggaaat tcaaatcatt ttctagtact gttttagaag
     6421 ttacagtgta ctattccaac ttcaataaac tataaacctt tgtctaataa caattttagt
     6481 ggagataaga aatcctacac ctaactcatc ttacacgtaa tctatttcta ttttatcaca
     6541 aaaaacgcaa gtaagaccat taactcaatt cagttttatc tgccattttc acaaatggga
     6601 aataagtcaa gacactaata atcaaacaaa caactgataa gatgatggca cgccaatcaa
     6661 atgctgtaga gaagaaacca tataaaattg gaggcattac ccaagtaacg ttttgtgtaa
     6721 caggtgaaac aagaccccag cttgttgccc agtaagctac cgttgccatg aaaaccgggc
     6781 taagtacaaa tggtataaat agcaaaggat tcaagacaac tggtaaacca taattgaccg
     6841 gctcaccaat attaaacaga actggtgcta gaccaagttt agcaactttt cgataatgac
     6901 tgtttcttga aaaaattaaa atagcaagta ctaatcctaa tcctccaaac cagacaaacg
     6961 ccccaaaaga cccacttgtc catatataag gaatcagttc acctttttgg aaagcatcca
     7021 gattcgctaa catagcaact ccaaacagcc cttccatgat gggagccaat acatttcctc
     7081 catggagacc aaaaaaccag aataacttat tcaaaaagat catcagaata actgcaaaga
     7141 aactttgaga caaacctagt aatggcgttt gtaacacctt gtaaacccaa tcaatcaata
     7201 agtcattgct aagtaaatgg aaaacataag tcaagatggc tactatatac atcgccataa
     7261 atcctggaat gatagaagtg aacggcttag caatcgcagg gggaactgaa tctggtaact
     7321 tgattaccca gttctttttc attactttac agaaaataat agaggctaaa aatccaatca
     7381 tcatggctgt aaagtagcct ctggcattaa tatggtttcc tggaatcaca ttcccaatag
     7441 ttaccatcag atttttacca tcaaatgcta gattatcaat tccttgtaag atttgatcta
     7501 atttcacatc tcctacattt gccaaaggga aactctttgt aactgtactt ccaatcgaaa
     7561 tgacaaacga agcaagtgat accaaaccag cagaaactgt atcaaccttg taaatcttag
     7621 cgatattcac tcccaagcaa tagatgaaca acaaggaaac aattggtata cttcccttga
     7681 ataccaaatt attgatgtca acaagccact gaaaggtttt cgtaatactt tctaggtgaa
     7741 attgttgtgg taaatccact agaaaagcat ttaataacaa agcaatggaa cctgtcataa
     7801 taacaggcat agtccccaca aatgaatcac gtaaagcaac taaaaacttc tgattcccaa
     7861 ctttcattgc aattggagaa agtttttctt gcattttatc caacattgta ttcataccaa
     7921 actcctaatt tttttcaaat cgttcgtata cttctaccac ttctttcgcc aagtcaacaa
     7981 aagtcagact cgtcattaaa tgatcttgcg cgtgtaccat caagaggcta attgatgttt
     8041 tttccccctg tgcctctctt gtcaacattt ctgtttgagc cttatgcgcc tgtaataagg
     8101 cagcattcgc atcagctaat cttcgattgg ctttagagaa atcccctttt tttgctgcct
     8161 gaatagcctc catagcatta cttttggctt ccccaccata cataattagc cccattacag
     8221 attctaaatt actttcatcc ataacattct cctcaatctt agtctaataa actttcagct
     8281 aaatctaaaa ctttagaacc attcatcatt ccgtaatctg tcatcggaat aaccgccaca
     8341 ggaatctgtc tatcttttag tttttcttga aagtccccta gtaaatagcg aacttgagga
     8401 cctaaaagca acacatttac ttcttttgtt gctacaattt cttctgcttc aggagctgga
     8461 actgcaaaaa tttctgcatc caacccctta tcttctgctg ccttttgcat ctttgtcact
     8521 agcagacttg tactcatacc tgctgcacat gctaacataa ttgttacttt agccatagct
     8581 tactcctttt attttattgt caatgttact gtttttcgat caactttaga ctcataatct
     8641 aaatgtttgg gacgactagt ataatgcaaa ttataaactc ctggaggcat ttgtgaaatc
     8701 ttttcttttt cagttttatt gcgaattaat aaatctaccg aagtattccc tccaaagtta
     8761 ttaaacatag gttcattttc aatagaataa tctaactcat atccactatt agttgtaatt
     8821 ccttttaata aatcaggaac atctcctctg tttattacta tatccttagc gccatttatt
     8881 ttgggagatt gactataata ctcttcagct tgatgttgtt gtaaatcatt tctagagtaa
     8941 gaaacataat caactacaaa ttcagtccct gataaattat tgtccactac cccaggccaa
     9001 cttcctccca tcgctaaatt taactgaaca tactgaggtc tattaaaaat ctttttcgct
     9061 atattatctg atccataata tacttttcta acaatatgat tgtcaaccat ccatacaata
     9121 taatctttat accaatcaat tgaaaaagta tgatacgaat cattaaagtt tccactcgat
     9181 aatttaaagg ctgtaccacg tcctgcaaat tttccatcat cattatccga ctgtccataa
     9241 tgaagcgttt gataaactgt cttattccca cttccatttg aatttcgttc accaactaat
     9301 tccattatat ctatctcacc tgttgacggc catcctcgtc cctgaacagg attaatcttc
     9361 ccatcaagtg taaaatccga tccaagagtc cagaaagcag gaaaaactcc ttgacctttc
     9421 ggtaattttg cacgcatctc taatttacca taaagaaatt ctactttccc atgtgttcta
     9481 acacttcctg aattatatac aacctttctg tgattgtttc ttgggttaaa atactgatct
     9541 tccttagagc gattcgtagc tctaagaact aaatttcccg aacgtagaaa aacattctca
     9601 tcacttctta cataatgttg ctgttcccaa ccacgaattg atcctaattc atatccccaa
     9661 tattttttat ttaattgttc gccatcaaaa tcatctctcc aaattttctt atatgaattt
     9721 cctttagaca gttgatacat ctctacatta tcaataaaaa tattagattt ttttgtagta
     9781 tcgtcttctg accatttcac taattgaatc aaaactttat cgtgtttacc ggaattgaaa
     9841 gaaaacatgt attttttcca ttcggttgta ttaactgttt gatcaacagc ttctccaatg
     9901 ttattttgaa caatctctcc ttgcttatta ttatcaaacc atcttgcggt aaaatatccc
     9961 tctgctccac taatatctac tttggcaaaa gcagatacta aataatcttc attaggttct
    10021 atatcaataa cttgtccaat atatccatct gctctattat cattaattgc agtagaagga
    10081 atcatacctg agtaatgtcc gttactataa ctctcatttg aattaattat tcccctattt
    10141 ctagagttcc aaaacagctc tccttgctca aaatttgaat tttttattaa attttcatta
    10201 gcaaaaactt tcgcatgaat aaatattaat aaaaataata taaataaact taaaattaat
    10261 ttcttattct tcattttatc acctatttac tgacctagcc aagcgagata ctgtatgtct
    10321 cactcctcta attgccttgc tcaatgcata aatattatcg actgttgcca gtcccccata
    10381 tcctgcgtca cctatatgtt gaatatcaac tccacaaatt ttatttctaa gtgcaatttc
    10441 cttgatagta tctgtatcag atgtttcttg gctagtacca atagcactca atacaagacc
    10501 acccttacta tgaacgagat caacgacttc acgcaactct tggtcatgaa aggctggaac
    10561 ggttccaact gctggaacaa gtatcacatc cgcacctgct tccaacaatt gctctgcgac
    10621 agaaagctct gccacaggct cattcactcc tgccccgtgc attttaccag ctataatcaa
    10681 acctgagaaa ttctctttag cagattgaac tgctttgata atctcttgat tgctaactcc
    10741 tgttccagga tttccagtca aacagacaaa gtcaaaacct aactcctcta ttcgattcaa
    10801 tgtttcaaca ctagcaacac gacctgcaac aatttcctgt gtttcctcta gcatctttgc
    10861 agatgggtca atcggttcca aatttacacc aattggacaa gcaacaagct ggtgtaagcg
    10921 atgaataaca tcatcacctg aactatccaa agcataaatt ttaggctcaa aaacatcaac
    10981 acaattcaat aaaatcatat cagctccaaa agctctagca atttcagaat tagttatatc
    11041 cccaacaaat gtctcacgag taactacatt ttcagataga acaactctac cttcacttgc
    11101 taaaatactt tgttttaatt cttcagcatt catctgtaat atctcagatg gatttgcact
    11161 aattaaacgt ttcattctca atcctccaat aacttaaacc gtttttcaat agctattaca
    11221 aatggtaaat aaagtaagac atccaaaaca atcaatacaa tttgtaataa tgaaccagaa
    11281 atacttcctg tagcaagtaa accacttata acaggtggca tagtccaacc tggatctgta
    11341 taagttagcg gaaccaaacc tgatgccatt gctccccacg ctactagtaa gttaaacatt
    11401 ggagctaaga taaatggaac tagtaacaaa atgttcaaaa caataggaac gccaaacatt
    11461 gctggttcat ttatattaaa taatccaggt ataacagtta taggtgccaa agtttttagt
    11521 tgtttactag ctttcttctt atgtgctaaa tatcctaacg caagaactaa acctatggtt
    11581 gcaccaccgc cacctatgaa tacaaaatta tccataaata cgctagtaaa aatatttgga
    11641 agagtttctc cagtttgata agctacttta tttgcatcta gattagctaa ccataacggt
    11701 ttcataactg cattaacaac atttcctccg tgtaatccta caaaccaaaa actactatta
    11761 aggaaaatta agataagaag accaataaca ttatttccta aaagtccaag tggacctccc
    11821 aaagctactt gagcaatttc atgaagatta ggtaaactta atttatctaa agttgcataa
    11881 acaatcaacc aaccaacaat agtcactgca ccaggaataa tggcactaaa gcttttagat
    11941 actgctggtg gaacaccatc cggcattttt atctgtatat tatgatttat aaaccattgg
    12001 tagatatagc cattaatcaa tcctaaaatc atagcaacaa ataaaccttt tgatgccatg
    12061 aaagcggtag gcattcctgc tccagcctct cctctaatga aaggcgtaac tgtaataaaa
    12121 gatgataatg acacaatacc agcaggaata ccatcaactt tgtattgtct agccataaag
    12181 taagcgatac caaaactagc tacaagacca agtaatccaa aactactatc aacacccttc
    12241 cataaatagt cagcaacacc aatatcaccc agatattttt cccatcctgg aataggaaaa
    12301 cttgcaataa ccattagtaa tgaacctatc aacaataaag gtatagttag agtaataccg
    12361 tctcgaatag aaaccaacgc tttattatta cccaatctag ccgctactgg aagtaacttt
    12421 tcttgtaata atttttccat aaccttttcc tcttaaatat attttattat caaaagttat
    12481 caattatctt tattttttga cgtcaagttg ttatttgatt atcttttgtt atttatattt
    12541 tacaactata agaaagcctt gtcaaggatt tgtgtaaaca tttgattatt ttttattatc
    12601 aatatataat aaaaagagac cagtaagtaa tgaaaagctt aaaaaaatta acatctacat
    12661 ttatccatag caaaaaatca taggatttta agaatctgac aactaggaag aaaactaata
    12721 tgaccttatc taagaaacaa ttacaactaa gagctaaaat actggaaaca gtttacacat
    12781 taggtcccat ttcacgaatt gaaatcgcta ctaaaacagg aataacacct gcaacaacta
    12841 gcagtatcac aaatgatctg attaaagaaa atattctctt agaacttggt gaagacgaac
    12901 acgatacaag cgtagggaga aaaaaaatcc tgctagacat tcaagctaaa cgattttact
    12961 acattggttg tgaactatct gaaaaacatt tcacttttgc tttaggagat aatctaggaa
    13021 atatcttaaa ggaagagaaa gaaattgtta ctaaacagtt aatccaagaa aagggaaatc
    13081 aactcattaa ccagacacta aagcaattct taaataactg ctctgattat gaaatagaag
    13141 caattggaat cgcgctacct ggtcgctatt tagataatta taaaataaca acaaacaatc
    13201 ctttatggca acatatcgac ttagaaatga ttcaatccca ctttgataag cctttgtttt
    13261 tctctaacaa tgttaactgt atggctatag gtaaacgcct atttagtcgc caacagaatg
    13321 accctaattt tgcttatttt cattttgcta gaggtatgca ctgttcttat atctacgacg
    13381 gaaacattta tgggaaagga aatctaatga ttggagaaat agggcataca gttgtttcat
    13441 ctgagggaga agaatgtagc tgcgggcgca agggatgctt acaaactttt gctggagaat
    13501 cttggttaat caaaaaatca aaaattctat atcatcaatc tccctattct ctacttccta
    13561 gtttagtcaa aaatgccgat gatattgata tccaagttat tttaactgct tatcaattag
    13621 gtgacactgg aataatcaca cttatccatc aagccttact ctatcttagt caaaccattt
    13681 taaatatcag tatgatgatt gactctcaaa aaatctacct acatagtcct ttactaacaa
    13741 atcaacacat catccaaaaa ctatatagtg aaatgaacta caaacccaaa ctgctctata
    13801 atcgcctacc tgaagtcatt attgaacctt acaatgattt tacagccgca cactcagcta
    13861 ttgcactttg tttgtatcat acaattctac attcttaatt ttctattaca tttaataaat
    13921 aggctaaaaa gagggagtat atcataaaaa gcgaataaac ctgttttctg aaaatcagaa
    13981 ttctgttttg ttcgctttct ttatatttgg aattatactc ctatctcccc tttttcataa
    14041 tgtatagtaa aatgaaataa taacaggacg aatcgatcgg gacagtcaaa tcgatttcta
    14101 acaatgtttt agaagtagag gtgtactatt ctagtttcaa tctactatat ttttgtacag
    14161 gtgcttcaac catttgaacg atttcaaatc cttctttttg gtaaagattc tgagctcttt
    14221 gatttgcctc gaagacattt agagaaatac tatctatatc ttcattttca aaggccaaac
    14281 taacaaattt ccttaaagcc tggctaccta agtcttgctc ctgtttctgg gggttgataa
    14341 aaaatctccc gatatgaaga ttgctgtctt ctagcctgat tttctggata agtcccacaa
    14401 actcttgttc atcaaagatt gaaaagactc cttccaaggc ttgaagggtc agtagaaaag
    14461 gaatccttgg tcccatccat tgttcttgaa aggatttgcc tagggagttg gaccactggc
    14521 atacaaattg agcgttttct atactcacct tttcttcaaa acgaattgtc atcttttcct
    14581 caccatctta tctatgtttc tccattatac tatttctccc attttttacg aatagataag
    14641 tatgattgat ttttattttt ttctcgtcgg gagcattctc gcttcctttc ttggtttggt
    14701 cattgaccgt tttccagagc aatccattat cagttcagcc agtcactgcg attcctgtca
    14761 gactcgcttg cgtcccttag atttgattcc gattctctca caggtcttca atcgctttcg
    14821 ctgtcgctac tgcaaagttc gctatcctgt ctggtatgcc ctctttgaat tagtcttagg
    14881 actcctcttt ctgctttact cttgggaatt gctttccttg gggcaagtcg tcctaatcac
    14941 cgctggtttg accttgggta tctacgactt tcaccatcag gaatatccct tactggtctg
    15001 gatgactttc cacctaatcc taatagcttc ctctggctgg aatctggtca tggtctcctt
    15061 ccttgctctt ggaattttgg ctcattttat cgatatccgc atgggcgcag gggatttcct
    15121 ctttttagct tcttgtgctc tcgtctttag cgtaacggag ttactgatct tgattcagtt
    15181 tgcttctgcg acgggtatcc tggcctttct cctgcaaaag aaaaaggaga gacttccttt
    15241 cgtgcctttc ctcttacttg ctgcttgtgg gattattttt ggtaagctac tgcttgtctg
    15301 ataaaatcca atttctgcca tatgtccttc atgaaattat ttcacagtta aattataaat
    15361 tatttctttt gtacaaaggg atgatgttat caaatcgatc tgttcttcta tcttcttgat
    15421 actgatcaaa aaatttcatt tcgactgaaa atatttcgct tataaactgt aaacgaatac
    15481 tttgtttaga cattatagtc gctagactga ctagatgatt actcaaaacg acgtccagaa
    15541 tactctttac tttgcttggt tttttaacaa aaatttgatc atccaaaggt tcaatcattt
    15601 tgtaaccttt ttgcgcaatt tgacgataaa agtaagaatg ttgctttgga gtcaataatc
    15661 ctaacttaaa agctcgatac tctaaagcct gtatcgaaac attcaaatcc gacttcaata
    15721 aaatataact atcaggattg ctgacacgct tgccaaccct ctcttcaaat ttgactaaaa
    15781 actcttcttt tggcaataaa aaacatgatg caaaataatt tgcttcttgc tccaaacgat
    15841 tgccatcttc attcatatct ttatatttat gtaaaagaat atgtcctagc tcatgagcta
    15901 agtcaaaatt tcgacgtaca gatgatttat tcgttcccaa cacaatataa ggtcttccca
    15961 attttgacca tgcgctataa gcatcagctt ggccattaat taatcgttcc acgatataga
    16021 tacctgaacg ttctaattta taaagcaaat catgattatc ttttgaaata cctaattttt
    16081 ccctggcata aagagccaat tcctcaatgg attctccctt atgataagat tcactcacta
    16141 cattacttag gtcatgaatt ataatattag gtataattac aaaactttca aaataatcaa
    16201 tcaaactatc taccttatgt aaatacatag tttgaatatc tattgttttc cgtgttgcta
    16261 ggtctgcatt tctaaaggca attacagaag aatcaaatcg aatgctctct tcttcctgtt
    16321 caaaataagt taaatcaaca tgaaattggt tggccaaatg cattttggtt gataatttag
    16381 gtttcgtttc gttggactca aactgccaaa tggcttgttc cgttaaatta attctctgag
    16441 ctaattctgc tctacttaaa ccatttaaca gccgtaattc tttcaatacc cgaccattaa
    16501 acatttatat actccttact acttttgacc ttcttgtttt tctattcttg gaataatttc
    16561 aaaatcttct gtttccgata attctgaaaa attaggaata tcttgatatt tagcttcttc
    16621 gaaatggtac gaacttgatt gaatatactc agacaaatct tgaatcaagt gtagctgacc
    16681 agttcgtgca tcaggcataa caacatctac agatataata ttgttttctg agtccgcctc
    16741 ataagttaaa atcataaatt tttcgatatt cgaattttta gtagcttgtt caatttcttg
    16801 aatcatttca tcagaaacta actccatctg aattggaaag gaatgactat cttcatcatt
    16861 tttgtaggaa gaatgttgat taagataaag tgtattcatc tgagcgtatt caaataagta
    16921 gccactctta tttttttgta ccaaaggaaa ttggttcgta agtcgcttct taccctttat
    16981 aattaacaat actttcccat atttttctgt atttgtttca aattctaaat atccccaagt
    17041 ctgtcctgct aattgtaatt tatactcaaa caaatctgct gatgcaaatg cagtatcaat
    17101 atgattaggt cgcgtccatg cataaccatt cgacactatc attgtctctc ttttttctag
    17161 acgttcatct acataatctt tttgcccttt catcaaagta tctacaattt tttgtgcctc
    17221 aagcgaatca aagagatcct gattcaacat aattcttcct cctccaaata ctttttaatg
    17281 aattatatca ttttcttaaa gaaattacta caataattat ctttttctta aagttctgtg
    17341 tcagagtaat ttagaaaatt atatcttcta tagtaaaatc aattaaaaac tgaacaaatt
    17401 tattgggaaa ttcaaatcgc tttctgaaaa tattttagga accgtagtgt aatattccag
    17461 attcaattca ctataaaact gacctttctc ctgcaaaaga aaaaggaaag acttcctttc
    17521 gtgcctttcc tcttacttgc tacttgtttg attatttttg gtaagctact gcttgtctga
    17581 taaaatcctg aatcggctct ccttggtgga gagcttttac tatttttgaa ccgacgataa
    17641 caccatctga caccgcattg aagcgttcca gatcggcttg actagataca ccaaaacctg
    17701 tcaagactgg gatgtcggcc acttgatgaa gttgcgccaa gtgcttgtcc aaatctgcac
    17761 ggtaattgcc tgatttccct gtcaccccat tgatggcaac ggcatagacg aatccctccg
    17821 cgccttcaat caactctttc tggcgctcaa ttcctgtggt caagcttact aaaggaatca
    17881 aggcgatatc tgtatctgcc aaaaatggtt ctacaaagtt ggcatgttca tgaggcaagt
    17941 ctgggataat caagcccttc acagctgtat cagccagatc tttgacaaag ttctccacac
    18001 cgtactgaaa gagggggttg aagtaggtca tgatgaccag tggaatctct gtttcaatgg
    18061 ttttcaaggt ttcaactaaa gcctgggtag aggtcccgtg ggctaaactg cgcaagccag
    18121 cttcttcgat aacaggtcca tctgcaacag ggtctgaaaa gggaataccc acttcaattg
    18181 cagagacacc caaatcttct agaaagtgga ttgtttctcc tagtccatcc aaacctttct
    18241 cgtggtctcc agccatgata tagggaacaa aaattccttt tccagctgct ttaatagcat
    18301 ttaatttttt tgttagtgtc ttaggcatga gcttctccct tctttgctgc atctgcttcc
    18361 aagcggtctt tgacttgaac cacatccttg tccccacgac ctgataggca gacaatcata
    18421 gacttttctg gtccaagttc tttggccaat ttcaccgcaa aggcgatagc atggctagat
    18481 tccaaagctg ggataatccc ttccacacga gacaagagtt ggaatccttc caaagcttct
    18541 tcgtctgtca cagggacata gctggcacgt ttaatatcgt ggtagtgaga atgctctgga
    18601 ccgataccag gatagtccaa acctgctgag atagagaagg cttcaagaat ttgaccatgg
    18661 gcatcttgga gcacatccat gagggaaccg tgaaggacac ctggacaacc cttggtcaag
    18721 gtagctgcgt ggtgctccgt atccacacca agtccagccg cttcagctcc atacatggct
    18781 actgactcat cttctacaaa gggatggaag agaccgatgg cattcgaccc accaccaaca
    18841 caggctacta gggcatctgg cagattttgt cctgtcaagt cacggtactg ttgtttagcc
    18901 tctcgaccaa tgacactttg gaagtcacga acaatttctg gaaatggatg aggccccaag
    18961 gcagaaccaa gaatatagtg ggtatcatcg atattagcca cccatgaacg aagggctgca
    19021 ttaaccgcat ccttgagcac gcgcgaacca tctgtcacgg cctcgacctt agctcccaaa
    19081 agctccattc ggaagacatt gagggcttgg cgtttgacat cttcctcacc catgtagatg
    19141 gtacattcca tgttaaagag ggccgcagca gttgcagttg ccacaccgtg ctgaccagca
    19201 cccgtttctg cgataatttt ctttttaccc atgcgtttgg caagccaaac ttgtcctaag
    19261 gcattgttaa tcttgtgggc tcctgtatgg ttaaggtctt cccgtttgag ataaatcttg
    19321 gctccgccga tatgctgggt caagtttttt gcgtaataaa gaggagtttc acgtcctacg
    19381 tactggcgca aaagctggtt taattcctct tggaaacttg ggtctgcctg actttcacgg
    19441 taggccttct ccaactccaa aactgctgtc atcaatgttt ctgggacaaa acgtccgccg
    19501 aattttccgt aaaatccatc tttatttggt tcctgatatg ccatgcttta ccctctctat
    19561 aaatcttcta atcttttcat gatctttttg tccatctgtc tccactccgc tcgatacatc
    19621 tactgcatag ggagtaaaat attgaattgc ttttactaca ttatcttcat taaggccacc
    19681 tgcgataaag aagggctgtg ctagtccagt cgtatccagt tgaccccaat caaagggctg
    19741 gccacttcct gccacagggg catcaaagag tagataatct gcctgagaat tagggacatg
    19801 cccatttcca tctacctgca cagcctgaat actggcacaa ggcaaattct caaataaatc
    19861 atctgccacc tgaccgtgaa cttgaaccaa gtccaagcca actttgtcaa tcgcttccag
    19921 cagttctacc cgacttggtg aaacaaatac tccaaccttt ttcacatctg caggaataag
    19981 ctttgccaac tcagctgcct cttctaaagt cacctgtctt ttactaggtg caaagacaaa
    20041 accgatatag tcggctcctg ctgaaacggc tgtttccacc gcttctttgg tcgatagtcc
    20101 acaaatttta acctttgtca atctgcaact ccttgattct ctgggccaca ttttctgcct
    20161 gcataagagc tgtccctacc aaaattccgt taaagtatgg ggctagtcgt tccgcatcct
    20221 gccctgtgaa aatggcagat tcagaaatgt aatagcgacc ttcctcaaag taaggggcta
    20281 aatctacact ggtctgcaag tcgacctcaa aggtagtcaa gttgcggttg ttgaccccga
    20341 taatctcagc accaagtctg tgggctacct ctagttcagc tagattgtga gtctccacta
    20401 agacttccag accaagctct gtcgcgtagt catacagttc cttgaggcgt tcttcggaca
    20461 aggctgccac aataagcaag ataactgtcg cacctgcatt gcgagcgcgg atgatttgct
    20521 tttcatctat gataaagtct ttgttgagcg tcggaatctc tacctgactg gaaatttccc
    20581 gtagataatc caaatgccct ttaaagaaaa cctcatctgt caacaccgaa atcatcactg
    20641 ctccgttttc ttcataagtc tgggcctgtt gcacaatatc cacatcgaga ttgatatctc
    20701 ccaaactagg gctagctttc ttgacctcag cgattacctg caagcagtcc tgatgattct
    20761 tcaaaaattc tgccaagcga taggtctggc gcagaggctg gatttgctcc agcttcatct
    20821 gctccacctc acgcgccttc tgctctaaga ttcgtgctaa aaattcctga ctcatttttg
    20881 gtactcctgt aacagtctga gtttttcaag ggccttgcct ctagcaatca cttgacgggc
    20941 caaggcaact ccttccttga tgctatcaat cttaccatta gcatagaaac caagaccagc
    21001 attcaagact gtcgtttcca agaatggact tgcttcgttt ttcagaacgc taagcaaaat
    21061 ttctgcattt tcctgagcat tccctccacg aatatcttcc atagcatagc cttccattcc
    21121 caaatcctct ggagtaaagc ttgacaagct gatttcgcca ttttcaagaa gtgcaatctt
    21181 ggttgttccg ttcaagccag cttcatccaa cccttctggt ccagcaacca cgatggcacg
    21241 tttgcgaccc atatttttca aaacctgagc tgtactttct aggagttctg gacgactaat
    21301 tccaagaagc tgtgtttcta aagccattgg atgaatcagt ggaccagtca agttcataat
    21361 cgttggaatt cccaattcca aacgagctgg catgatgtat ttcatagctg ggtgcatatt
    21421 tttagcgaag agaaagacga tgccagtttt atcaaagacc ttacctagtt cagctggttt
    21481 gaggtcaaga ttgattccca aggcttcgag gacatctgcg gaaccagatt tagaagaaat
    21541 cgagcggtta ccatgcttgg ccatgtgaat accgccacca gccaagacaa aggctgcagt
    21601 tgtggaaata ttaaaactga aagacttgtc cccacctgta ccacaattgt ccatggcatc
    21661 atgaatctca gttggaatat gctgggcatg tcctctcatg acttgggcaa tggctgtgcg
    21721 ttcttcaggt gtttccccct tcatcttaag agctaagagg agagaagcaa tctgcgcttc
    21781 agttacacgc ccagttatga tacgctcaat gacatccgtc atttccacac ctgataaatt
    21841 ttcaaatttt gctagttttt caataatctc tttcatccta gtttcctcac tttacaacct
    21901 cctcgataaa attccgaata gaagacaagc cgtctggcgt tccaatgctc tctggatggt
    21961 actggaagcc ataaatcggt aggtttttat gttgaatccc catgatggtt tggtcatcag
    22021 tcgaacgagc tgtcacttca aagtcttctg gcatttcctc aatcaaaata ctgtgataac
    22081 gcatgaccgc acgtccatcc tcaatacctt gatacaaaac agatggcgct tcaaagttga
    22141 tattgctctg tttcccatgc atgacttttg gagccaaacc tagcttacca ccaaagactt
    22201 ctgcaatggc ttggtggccc aaacaaatcc ctagaatcgg cttcttgcct gcaaaatcac
    22261 gaatcatgtc ttccatcttt ccagcatcaa ctggccaacc aggaccagga gaaaagacca
    22321 gaccatctgc tttttcagct tcttcataca gcttggaatc atcatttctc agaacctgaa
    22381 cttctgcaaa attcccaatg tattgggcca agttataggt aaaagaatca tagttgtcaa
    22441 tcaataaaat catggtctta gttctccaat tctagtcata gattttgctt tgttaatggt
    22501 ttcttggtat tcgttttggg cgatagagtc gtagacaatc cctgccccag cctgcacata
    22561 ggctctttga tttttgagaa tcatggttcg gatggcgatg gccaaatcca tatcacccgt
    22621 cgcagacaag tagccgattg ccccagcgta tactccccgt ttttccgttt ccagttcata
    22681 gatacgtctc atcgctcgaa tctttggcgc tccagaaacg gttccagcag gaagcgttgc
    22741 tttcaaggca tccatggcag tgagttctgc aagcaaacgt cccttgacca cactggtcaa
    22801 atgcatgacg tagcggaaga gctccacctc catatactta gtaacttgga cactggccgt
    22861 ttcagagatg cggccaatat cgttacgccc caagtctacc aacattcgat gttctgctgt
    22921 ttccttctca tcagagagga ggtcagtcgc caaggccttg tcttcttcat ccgtagcccc
    22981 tcttggtcgc gtccctgcaa tcggattggt tgtcacgatg ccatttttga cagaaaccaa
    23041 actttctgga ctagctccga tgatttgata atccccaaaa tcatagaaat aaaggtaatt
    23101 agaaggatta gttacgcgga gatttctgta gaagtcaaat ggatttccag taacttctgc
    23161 tgagaaacgc tggctgagta cacattggaa catatcacca ttacgaatca agtcacgagc
    23221 tgtttccacc atttcctcaa acttctgagg agcgatatgc ggtttgaagt ctaacggaga
    23281 tagatccaag tcttcaaact catttggagc aggaatgcgt aattcctcaa gcacttggtt
    23341 caaggctttt tccaattctt cttgactacg ctcactatag agagcatcct cgatgacatg
    23401 tatcttctcc ttcttgtggt caaagaccat atagctctca tagacaaaga aatgcatgtc
    23461 tggcgtccca attgtatcct cagggatttg accaatttct tcataaagcg aaatcatatc
    23521 gtaacccaca aaaccaatgg ctccaccacc aaaagggagc tctgagtggt gctgactctt
    23581 atgaatcact tcataaagga aatccaaggg atcccgatca atcacttgac cattttgata
    23641 gagaactcca ttttcaaact taatctcaaa aactggatta taggctagga tagaaaaacg
    23701 agccgtttcc ttgtctctcg gaatactctc taaaataacc ttatgttgcc cctttaagcg
    23761 catataagcc aagattggtg ataagacatc tccatgaatg attcgttcca ttgtaatttc
    23821 cctttcagtt ctaattctag ttcgtggcga ctgtatgaaa aatccccacg caaaataact
    23881 tgcgtgagga cgaaattcgc ggtgccacct caattatagg atttctccta tctctcattc
    23941 ctgtctcaga tacctcctgt aacaggctgt gcgataaagg gcactccctt gagaatgatg
    24001 ttttcttctc tcgtttcaga tgaacccaac tttacagctt tctctgcttg ttttcagcaa
    24061 ccacaagctc tctgtgagag aaaagactgt aatttttcca tctattattt tttagcttct
    24121 agtaatctgc aatcgcagct aggtccttgc ctccacgacc agagacattg atgaagagat
    24181 gttcatctcg gtacaccttt atactcttcg aaaatctctt caaaccgcgt caacgtcgcc
    24241 ttatcgtagg taacgacgga tagaaaaaag cccacacaca gaatatactt ccgtgtgagg
    24301 gcgttggtaa cgcggtgcca cctcaattat aaagggacta tccctttaca tctctgcctt
    24361 gtttaacaac aagctgcact gtaaggtgtg cgcaccgaat tttcattgtt tcaaattcat
    24421 tttcaaaatc agcccacttt cactacttcc aaccacctat tcacaatcac cacaggctcc
    24481 ctgaagatca aaaatagtta cttttctgat ttgttgaact tattttaata ctttgttttt
    24541 tctttgtcaa gactttttta cgattttttt gatatcattc gaatgtgacc atatcttcct
    24601 tagatcgaac atgaacatgt cccacttgtt agaaattgga tccaactcaa tagaaactga
    24661 atggaggcta aacagaactt attttagaac actccatctt ttccactagg attttcaaga
    24721 attaaacaat actagaaact ctgtctccta acaaatttag gagaaacttc aacagatgtg
    24781 acactttccc ctttaataat tgctaaaaca ccttctatca tttctttagc caatttaaca
    24841 taattgggag caattgtaga caaagctgga gtataatact gagaaatagg aatattatca
    24901 aatccaatga tagaaatatc atctggaata agaattcctt tctcatagca cgcacgaatc
    24961 aagccctgaa ccttttcatc tcctgaaaca aaaataatgt ccggataatt ttgggtagtc
    25021 aagtgctgca ttgcataaga ataaactgaa tcaattgtag ataagccata aatgactttt
    25081 aaatccataa agtaattttt atcattcaga aaagaacgca cacctctttc acgatcctta
    25141 ttaacatgag attctcctcc cataagcaac cacatatttt taaatttttc ttcagttaca
    25201 gctttcatca tatcataagt agcttgaaaa ttattattag atacatagac tactccagac
    25261 gtttgagatt caccgaaaac aagaaaaggc atatggttct tctttaaata ctgaattctg
    25321 atatcatcta cactttcata aaaaacaata acaccatcta ctaggctacc tgtgcttgat
    25381 ataattgaat tactaattgt atcctcctct ccaaagtact caactatagc attaacacca
    25441 aattctttac acgtccgtaa cactttatct aacagcgtat gaaaccaaat taaaggaaaa
    25501 gagtcgattt tttttacaga aatcaatata tttatagctt cttttttagt taaatttttt
    25561 gcatacgcat ttggaatata cgacaattcc tctataactt tttgaatcgc ttgataagtt
    25621 tcttctttaa catttactcc accattaata actcgtgaaa ctgtttttgg agaaaaacct
    25681 gataaacgtg caatatcata aatagttacc tttttcccat ttatattttt catttcagtc
    25741 ctccattacg aacattctaa tattactata caatatttaa ttttttttaa caagagaatt
    25801 tagt
//
LOCUS       DAVKQB010000030        25295 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKQB010000030 DAVKQB010000000
VERSION     DAVKQB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25295
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.030"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(154..525)
                     /locus_tag="VW528_001274"
     CDS             complement(154..525)
                     /locus_tag="VW528_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEU9912092.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
     gene            1283..1603
                     /locus_tag="VW528_001275"
     CDS             1283..1603
                     /locus_tag="VW528_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9912093.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSILFHLKKEVEIYYQ
                     AKER"
     gene            complement(1892..2362)
                     /locus_tag="VW528_001276"
     CDS             complement(1892..2362)
                     /locus_tag="VW528_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU9912094.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(2328..3134)
                     /locus_tag="VW528_001277"
     CDS             complement(2328..3134)
                     /locus_tag="VW528_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9912095.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(3350..3991)
                     /locus_tag="VW528_001278"
     CDS             complement(3350..3991)
                     /locus_tag="VW528_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912096.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(3988..6096)
                     /locus_tag="VW528_001279"
     CDS             complement(3988..6096)
                     /locus_tag="VW528_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9912097.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(6148..6435)
                     /locus_tag="VW528_001280"
     CDS             complement(6148..6435)
                     /locus_tag="VW528_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9912098.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            6860..7180
                     /locus_tag="VW528_001281"
     CDS             6860..7180
                     /locus_tag="VW528_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9912099.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICTDNAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            complement(7233..7805)
                     /locus_tag="VW528_001282"
     CDS             complement(7233..7805)
                     /locus_tag="VW528_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9912100.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            8047..8718
                     /gene="sdaAB"
                     /locus_tag="VW528_001283"
     CDS             8047..8718
                     /gene="sdaAB"
                     /locus_tag="VW528_001283"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9912101.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            8727..9599
                     /gene="sdaAA"
                     /locus_tag="VW528_001284"
     CDS             8727..9599
                     /gene="sdaAA"
                     /locus_tag="VW528_001284"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9912102.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            9621..10253
                     /locus_tag="VW528_001285"
     CDS             9621..10253
                     /locus_tag="VW528_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9912103.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            10303..10380
                     /locus_tag="VW528_001286"
                     /pseudo
     CDS             10303..10380
                     /locus_tag="VW528_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(10646..12496)
                     /locus_tag="VW528_001287"
     CDS             complement(10646..12496)
                     /locus_tag="VW528_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9912104.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(12538..13695)
                     /locus_tag="VW528_001288"
     CDS             complement(12538..13695)
                     /locus_tag="VW528_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9912105.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKEDIEILFINALSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRTIIYLDKISQINQNML"
     gene            13753..14919
                     /locus_tag="VW528_001289"
     CDS             13753..14919
                     /locus_tag="VW528_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9912106.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            15058..15384
                     /locus_tag="VW528_001290"
     CDS             15058..15384
                     /locus_tag="VW528_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9912107.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            15371..15964
                     /locus_tag="VW528_001291"
     CDS             15371..15964
                     /locus_tag="VW528_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9912108.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYATRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            15957..16868
                     /locus_tag="VW528_001292"
     CDS             15957..16868
                     /locus_tag="VW528_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9912109.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            16931..17995
                     /locus_tag="VW528_001293"
     CDS             16931..17995
                     /locus_tag="VW528_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912110.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVPQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(18173..19033)
                     /locus_tag="VW528_001294"
     CDS             complement(18173..19033)
                     /locus_tag="VW528_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9912111.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFERVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(19308..20294)
                     /locus_tag="VW528_001295"
     CDS             complement(19308..20294)
                     /locus_tag="VW528_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9912112.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEEREQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(20566..22041)
                     /locus_tag="VW528_001296"
     CDS             complement(20566..22041)
                     /locus_tag="VW528_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9912113.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(22251..23174)
                     /locus_tag="VW528_001297"
     CDS             complement(22251..23174)
                     /locus_tag="VW528_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9912114.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(23188..24117)
                     /locus_tag="VW528_001298"
     CDS             complement(23188..24117)
                     /locus_tag="VW528_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9912115.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGTLWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(24436..24656)
                     /locus_tag="VW528_001299"
                     /pseudo
     CDS             complement(24436..24656)
                     /locus_tag="VW528_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24610..24870)
                     /locus_tag="VW528_001300"
     CDS             complement(24610..24870)
                     /locus_tag="VW528_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912116.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         7692 a         5442 c         4495 g         7666 t
ORIGIN      
        1 tcaattggta aaaaatagta attccaaata aaatgagaaa tcagcaatga tagaaaaata
       61 acaatatcaa ttttcctgtt gttgacatac tctgtaattt tatgatacag gaatgcaaca
      121 ataactgaaa ttaaaacaat caacataaga ttattaacct attctcaatt gattatcacg
      181 tcgcccatcc cagccatcaa taatattggt aaaaatccaa tcagctccag caccaattcc
      241 agcagatgca atacttctaa taacagtatt tgcaaaagca tttccaatat gaattcctaa
      301 tactcctaaa cctttaacaa tcccagctac cagtgcggag cgtaatccat aagatgcaag
      361 aactagttga aggcctccac ctcctgctgc tgctaatgct gcatatgctg caccagtaat
      421 ggctcctgta attgcccacc tgggtatata tatagccccc ccatcaaggt acatcatctc
      481 ttcttcatca atcacaacat aattatttgg taatacaagt tccatttatt ttctcctttt
      541 ttattaagca tgcttaggat ggtttcctaa caagtgatcg caggctttgt ttagttacct
      601 aaattagact tccttccttt atcttatttt aaatatttaa aaccagttac tgcttgaagc
      661 ttaacagttt acttaattca aataacagct aatcactgat tccctgattt tatcaaatat
      721 actaataaca acaattgtga tacgatcatc attataagaa caacatttaa cgaatcaaag
      781 tttttccgag ataataataa agcaacagca agcagtatta ttactaaaga ttgaataact
      841 actattactc ttaacatttt atcctccact aacaactcaa gctcccttac attatatcga
      901 ccagccccat ggaaagatta tagctagact aatcaatgat ataatacata ctagaataca
      961 aaatattttt ttattcatac tttttctcct atctttattt tctacctaca tcatacctct
     1021 tattaagctc atttcaaaat tacgttaact tttttcacga aaaggagaca attatgcgtt
     1081 atgattatgg atagcgcttt caaattttta aactcctatc ccatcctttt atctatatta
     1141 tgagcgaaaa tataataact gtcaagtaac taaagtgaat tttataaaaa aattacaagc
     1201 caaatttgta aagtttacac taagccgcta gggcaatcgt ctatcagaat gtccgtttat
     1261 ttgtcaataa tccgagaaaa tcttgcaacg cttagaagtc tataaaaact atcaacattt
     1321 atatgacttg cgaatagcaa tcctgctcaa cctttccaca ctctatctat acaatcaaga
     1381 taaaaacatg tgtaagcaaa tctgctacac tttactggag gacgccaaga ataagaaaag
     1441 ctacgatagg ctggctatct gctatgtccg tattgggatt tgtagggatg atgcgaaact
     1501 tatccaaaaa gggttctccc ttctggagct aaccgaggaa acttctatac tgtttcatct
     1561 caaaaaagaa gtagagatct attaccaagc gaaggaaaga taaaaaagcc gagggatttc
     1621 ctcgactctt tacatattat ttgttcgtta gttcttaata ccagctattg ttaagccaga
     1681 agtttttagc tctgccaagc cacatagtgt gtttactact atgtgtccat gtcaccatga
     1741 atcgccgaac gatttaacga gaaagtatga cttttccgtt tatcccaact caattatgac
     1801 atttttttca aaagtcaata tatctcactt tttcaacgac aagaaagagg ctgataatct
     1861 accaacctct tattctgaac ccatcactcc atcacttttt agcttcattc gctttcttag
     1921 cgactgcaat ctggtattcg acttggtcat tccccttacc ggtacaacca tgagcaattg
     1981 tagtcgctcc tatctgatgc gctatttcaa ccaatttttt agaaatcaga gggcggctca
     2041 aggcagatac caagggatac ttttgttcat aataggcatg tgactgaaga gccactagca
     2101 cataatctgt agcaaattcg tccttaacat caatgacata agattctact gccccaacct
     2161 taagagcctt atcatggatg aaatctaagt ctttcccttc acccacatcc atacaaactg
     2221 aaacaacatc atagtctttc tttaaccagg taatagcaac tgatgtgtcc aaaccgccag
     2281 aataggccaa aattactttt tctttactca tgttcttttc cttctttcta tttttcaatg
     2341 gatgctttaa aggcatcctc aatgagtttg tctaattccc cagactcctt caacttttga
     2401 atggttttat cgactgcctc tttcaattcc ttgctatctt ttttcatggc taccgcgtag
     2461 gaatcatctt gctctttttc aaaattgagg tctgcgattg ctaaatcagg attattttcc
     2521 acaaatccct tggaaacagg ttcttcaaag ataacggcat ccacttgtcc tgattttaaa
     2581 tctgtgatta aattcccatt tttaggcaga gatacgaggg aagaattttg tagcaaatct
     2641 ttcgccatcg tctcttgaat cgaacctttc tgcgctccaa cctttttctg cgccaagtcg
     2701 tttacagact gataagtagt caagtcagat tttttgacaa tgagtttatt ttttgcagta
     2761 tagtagggaa tggaaaagtc aaacaccttg ctccgttcat ctgtcttaga aacacctgat
     2821 atggcaaggt ctgcttttcc tgattgaaca ctagccagta cattatcaaa actcatggga
     2881 gatagttcca attcgacacc tagttctgtt gcgatagcct tggctaattc gatatctgaa
     2941 cccacaatct gatttttccc atcaaccact ttttgatatt caaatggagc aaaatctgga
     3001 ttgagggcta caaccaattt tcctttggac ttgatggctt caattccagc cgattgattg
     3061 ttactacaag caaatagtag ggggagcatc actaaaccaa acatcgtcat caacaccttc
     3121 ttcatcttat tcataacaaa gccttcttta tgtttattct tttttattgt ataaataata
     3181 ctcctatact catatactca tcctttgtca agcctaaatt gaaaaaattt tcaatttgaa
     3241 acaaaaaaaa acctagaaat gctacataaa atgtcattcc taggcgtatt tatgctattt
     3301 ttctctggaa aacatgagta ttcagccagt caaaaggagc tgaatgaact cattttccct
     3361 cgcccaattc aatgacacga tgacattgtt gggctacata agcatcgtgg gtcacaataa
     3421 tgactgtttt cccttctcga ttcatctcta agagaaactt caagaccaaa tctctatttt
     3481 caggatccag cgaacctgta ggttcatcgg ctaaaatcag ctggctgggt tttaagatgg
     3541 ctctagcaac tgcaattcgt tgttgttcgc ccccagacaa ctcggagacc ctttgatgca
     3601 aagtagctga caaacctact ctctctaaaa tctcttccac ctttttgagc ttgtctttct
     3661 taggcaattt cacatatttc agcgccagca tgagattgta ctcgaccgtt tcatcatcaa
     3721 tcagggcaaa attttgaaac agataagaga tatgttcacg gattattgtt tgcgacttag
     3781 cagaattaac cgctagattt gtctgaccaa aaatctcata ccgtccgcta taatcaccat
     3841 ctatcaaacc caataaattt aacaaggtcg acttcccact accactctta ccaacaatag
     3901 ctaccaaatc cccctgatca atcctgagag ataagttatc caaaatcact tttcccccaa
     3961 tggttttggt aatatttttc aactcaatca taagatgccc cctttcaata actctactag
     4021 acttcttttc tccatcctag aagctaagcc tagcacaaat agtatatcca gacatgtaaa
     4081 acctgcaaac agtagaagtg gtaagaacgc atgggcaaag aaaatcaaga ctagaagagg
     4141 gaaactatag cccagcaaga gcagaacgag gagaggacgg tagcgatcga ccagtttcca
     4201 ccccataaac ttcttggtaa tgatatccct gcgcttcaat aagaaagttg ttactagtaa
     4261 gaagtaggaa atcatcatgc taaggagacc aaacaaagca aagagtaggt taaaattccg
     4321 aacagcatct ctataagaat ccactttctc ttgttgaatg gcttgaatag atgaaaattt
     4381 taaataattt ccatctgaca atttctcaac taactctgta atctcttttt gatgttgaac
     4441 cgtattttca attttaatcg gattatttaa gccagttgtt gacagggagg ctttctcatc
     4501 ccacatcata tcagaatcat tgaccaagct aataattgga ttggagagat tttcctttcg
     4561 cttatcacta tatgggaaaa atgaccaatc tccttcataa taggcaatct cgacatccat
     4621 ctcctctatc gttcgttttt gctgctcttc atacttcatc gaatgaaagg caattaactt
     4681 ccccaagagc tgatttttat cttcttcacc tttcgtactt gctggcatca aaataacttt
     4741 tttaataccg gtatctggta gcttgaatcc cttgctcttt agaaaattgc gattggcata
     4801 gtaaacatcc accgtatctg ttaactgata ttgctgaatc tgttctgatt ggacaaaatt
     4861 ttttacagga agactgctac tctgcacata gcccgcctgc gttttttcta ccaaatcctg
     4921 ataaaatcga tagaaataat ctgtagattt ccctgaccct gctagctctt cttgccacag
     4981 attatcattg agtttgaagg tttctaaggt caggtaatta ccttgactta cccactgttg
     5041 ctgataagca agttctttgt tttcttgttc taaacttctg cccaccccaa tcagtaaggc
     5101 cgtcagtaaa atagttgtcc ctattttcat cacataattg aagataagac caaatttgaa
     5161 agatgaaaaa cctttcagca gagagctgat tgtcattttt tggattaaga ggtaagtcaa
     5221 ccaactgata aagagataaa gctgcaacag caaaaaatga gacaaccaca gcataggaaa
     5281 caaatctttt ggcttataat caagcaagaa aaacacgcct agattgatca caagagcccc
     5341 acctaggagg aggtaaaggt tgccttttac aacatcagct aaaacagccc tatcttgaaa
     5401 accaagtaat ttttgtaccc caactctttt catctccatc atcggttgat acactgtcac
     5461 taacacaaga agcaaaatag ccaagacaaa aacaatggca gataaaagca aatctcgatt
     5521 tatgacttcc actgcacttt tgtaggtcgg ctctagcaag gtagcctggt ctatcttgaa
     5581 aaaatcgctc catttctgta caatcctatc cttgtccatc tcttgtgtag aagttatcgt
     5641 atagcgacca tttaaactac gagatgtatc cttgatatag gtttgaaaag tcataagctg
     5701 aataggtttg gcttttagaa aggtcggaat cgtaccaagt ttattggaaa tttctttatt
     5761 actatagact ccttcaccat ctgtggtaaa atcaagagaa gaaatcccaa actcttggta
     5821 ggggaaggta tctttatcaa aaacaccaga cttgaccacc tcatcaccac tgtctgtttt
     5881 gatgatggag actttatact cctttgatac atcctcaaaa aatcgaagaa cagacgctgc
     5941 aggttcgtta atatctttca aatacaaatc caaagaatct acagtctttc ccatttcttg
     6001 aatccgaacc acttctcgtg tatagtttac attaaaaaca aaaaaagtgg tacacagaag
     6061 caggagtaac atacagaaat tactgatttt tttcataaaa gtgcctccac tacagttttc
     6121 cctctccaaa taggagaggg aagaatttta ctcaaatcca tagaaatatt ccattcctgt
     6181 tggcggaatc ttggtgaggg aagctctagc ccatgcctca ggttttgcat aatccttaga
     6241 ggttctaccc ccatgtctaa cagttgctgt atgatatcga gtagaatgta agtaatcaga
     6301 atatccaaaa gttcctgtcc aacctactcc atagttccat tgaccaccct caacccatac
     6361 tgctaaagca tttgtagtag caagtatacc acataccatt aaagatgcaa caccaatttt
     6421 aactaatttt ctcatgattt atttctccta ttatcgagag ctgttccgta aaaacagcta
     6481 tagtaattta tcaacaagta tcgtagtcca ttggactcac cttccttcta tctcatttca
     6541 ttttttaaca acgaacttac tcttaaccgg gagaatggtg cgcttctgta tcaggtcctt
     6601 gaacaaccag agtagtatta agcacactct aactattact caagaatcag tccaataaac
     6661 aatatggata gcgctttcaa atttttaaac tcctatccca tccttttatc tatataataa
     6721 gcgaaaatat aataactgtc aagtaactga agtgaatttt ataaaaaaat tacaagccaa
     6781 atttgtaaag tttacactaa gccgctaggg caatcgtcta tcagaatgtc cgtttatttg
     6841 tcaataatcc gagaaaatct tgcaacgctt agaagtctat aaaaactatc aacatttata
     6901 tgacttgcga atagcaatcc tgctaaacct ttccacactc tatctataca atcaagataa
     6961 aaacatgtgt aagcaaatct gctacacttt actggaggac gccaagaata agaaaagcta
     7021 cgataggctt gctatctgct atgtccctat tgggatttgt acagataatg ctaaacttat
     7081 ccaaaaaggt ttctcacttc tggagctgac cgaggaaact tctatgctgt ctcatctcaa
     7141 aaaagaagta gagacccatt atcaaccaaa gaaattataa aaaaagtcga gggagctcct
     7201 cgacctttta catattattt gttcgttagt tcttaatacc agccattgtt aagccagaag
     7261 tttttagcag cagtccatga accgtaacgt cctgcaacgt aggcatctgc tacacgttct
     7321 tggttttcag ctgagtagtc accgttcagg tatgaatctg ttaattggta acgtccgata
     7381 taacgtccat ttgtagctgt atagctacca cctgattctt tttgagcgat ccattctttg
     7441 gcttctgctt cagatccgct tacagttgaa acaactgttt cacttactac tggagctgaa
     7501 acagtttcat cttgagcggc tggtgccgca taagtagctg gcgctggtgt tgcaacaggc
     7561 gctacagggc catcgataac caactcttga tcaacataaa tcaaatgaat gttatcaatg
     7621 tggttgtttt ctgccaattt ttcaactgtt gtgttgtgag tttcagcgat ttctgaaagt
     7681 gtatcacctt ctttaacagt gtaagttgat gattcttgag cagatacaaa tgatggagca
     7741 aatactgcaa acaaggcagc tactcctgca agagttgctt taatcttttt agttattgat
     7801 ttcatattcg aaaattctcc ttctttctat agttctatca taccctttaa atattaccgt
     7861 ttcttgacac ttttatgtag aaatattaca aaattatttt ttatttccct aaataaggtg
     7921 ttttttgtca taaaagatac tatttttatg ctatttgagg tattagaaag gttttcatcc
     7981 acaattaaag ccaagcccct cattctttga tataatagag ataagaattt ttataagggg
     8041 aaactgatga aatcacttcg ttttcaatct gtctttgata tcatcggacc agttatgatt
     8101 ggcccatcta gtagccatac cgctggtgct gttcgtattg ggaagattgt ctcttccatt
     8161 tttgatgata ctccgacaga agttgaattc caacttttta actcatttgc caagacctat
     8221 cgtggtcacg gaacagacct agcccttgtt gcaggtattt taggaatgga tacagatgat
     8281 cctgaaattc caaatagtct ggaaattgcc cacaagcgtg gtatcaagat tgtctggacc
     8341 attcagaaag acagcaatgc ccctcaccct aacaccacta aaattactgt taaaaatgcc
     8401 cacaagacca tcagcgtgac tggtatttct atcggtggag gaaatattca ggtaaccgaa
     8461 ctcaatggtt ttgccgtctc tctcaatatg aatacaccga ctatcatcat cgttcatcaa
     8521 gatattccag gtatgattgc ccttgttaca gaggcgcttt cccgctatgg tatcaatatc
     8581 gcccagatga atgtcactcg tgaaaaagct ggtgaaaaag ccattatgat tatcgaagtt
     8641 gacagtcgca actgtgatga ggctatcgaa gaaattcgaa aaatccctca tctccacaat
     8701 gtcaatttct ttaaatagga ggaagcatgt tttattctat caaagaattg gtcgagcaag
     8761 cagatctaga ctttcaagga aatgtcgcag aactcatgat tacaacagag tttgaattga
     8821 ccggtcgcga acgtgaagaa gtcttccttc tcatggaacg caatctggaa gttatgaaag
     8881 cctctgtcca acttggcctc aatgaaaata aatctcgtag tggcctgaca ggtggagatg
     8941 ctgccaaatt ggatcactac atcgaaaacg gaaaaactct gtcagattac acgattctct
     9001 ctgctgcccg aaatgccatc gcagtcaatg aacacaatgc caaaatgggc ttggtctgtg
     9061 ccactccaac cgctggaagt gctggctgtc tgccatccgt tctcactgct gctattgaaa
     9121 aattagacct cagccacgag caacaattgg atttcctctt tgctgctggt gcctttggac
     9181 tagtcatcgc aaacaatgcc tccatctcag gtgctgaggg tgggtgtcaa gctgaagttg
     9241 gttcagcctc tgctatgagt gctgccgcct tgactctggc tgcaggtgga acaccttatc
     9301 aggccagtca agctattgcc tttgtcatta aaaatatgct aggcctcatc tgtgaccctg
     9361 ttgcaggttt ggtcgaagtt ccctgtgtca aacgtaatgc catgggagct agctttgctt
     9421 tcatcgcagc agacatggcc ttggcaggta tcgaatctaa aatccctgtg gatgaagtga
     9481 tcgatgccat gtaccaagta ggagcaagca tgccaactgc ctttcgtgaa acagctgaag
     9541 gtggactcgc taccacccct actggtcgtc gcctccaaaa agaaattttc ggagaataag
     9601 cttatcaaat aggagaaacc atgacctcta tcacagcgat ttttttcgat ttggatggaa
     9661 ccctcgttga tagttctatc gggattcaca atgcctttac ctataccttt aaagagctgg
     9721 gggtgcctag ccctgatgcc aaaactattc gtggttttat gggaccacct ctcgaaagta
     9781 gttttgcgac ctgcctgtcc aaagaccaaa tttctgaagc cgtgcagata tatcgttctt
     9841 actataaggc aaaaggcatc tatgaagctc aactctttcc tcagattata gacttgcttg
     9901 aggagttatc gagcagttat ccactctaca tcaccacgac aaaggataca tctactgctc
     9961 aagacatggc aaaaaacttg gaaatccatc atttctttga tggcatttat ggttctagcc
    10021 ctgaagcacc ccataaggca gatgtcattc accaagcctt gcagacacat caactagcac
    10081 cagaacaagc catcatcatc ggagatacca agtttgatat gctgggagct cgagaaacag
    10141 gtattcagaa attggccatc acttggggat ttggagagca agcagatcta ctaaactatc
    10201 aacctgatta tatcgctcac aaacccttag aagttttggc gtattttcaa tagcatagac
    10261 tgggtaacaa ttccatttca gaagaaaatg aagtaatact caatgaaaat caaagagcaa
    10321 actaggaaac tagctgcggg ctgctcaaaa cactgttttg aggttgcaga tagaactgac
    10381 gaagtcagat caaaacactg ttttaaggtt gtggatggaa ctgacgaagt cagatcaaaa
    10441 cactgttttg aggttgcaga tagaactgac gaagtcagat caaaacactg ttttgaggtt
    10501 gtggatagaa ctgacgaagt cagtaaccat atacggcaag gcgacgttga cgtggtttga
    10561 agagattttc gaagagtata agtctctaca taataaaacg catattacca aggttgtgta
    10621 actggatact atgcgttttt cttttttatt caatgtgggt tgtttgatta gcaaaggcga
    10681 taatagcttg cttcaactca tctccactga gagtgcggaa ctctccaatc ttttgattga
    10741 tggattctaa aggcatgaca ttaaccttac caacgaaaat cttatccata acttgattat
    10801 caacgagttc ggttgatacc aagagttctt cgtagagttt ttcacctggg cggattccaa
    10861 cttcaacgat tggaatttca ctttcagtgt ggccacttag aagcaccatc ttcttggcca
    10921 agtcataaat cttgactggt ttgcccatat caaggataaa gacttcccca tctttggcat
    10981 aagcaccagc atggataacc agacggctag cttctggaat ggtcataaag taacgggtca
    11041 tgcggaagtc tgtcaccgtt acaggcccac cttcagcaat ctgacgttca aagactggaa
    11101 tgacactacc acggctacca agaacattcc caaaacgaac tgcacagtag gttgattggc
    11161 tacgttggtt aaagccagtg acaatcaact ccgccacgcg cttggttgct cccataacat
    11221 ttggtggatt gactgcctta tctgtcgaaa tcataaccat cttagacact ttagcttcat
    11281 caacagcctt agcaacattg taagttccac ggatattgtt tttgaaggct tcttttggat
    11341 tgcgctccat cataggaaca tgcttgtggg cagccgcatg ataaacaata gcaggtttgt
    11401 actgctcaaa gacttgcaac aaacgatcat agtcttgaat gtccgcaatc acaggtacat
    11461 aatcaatccc ttggaactta cgaatcaatt catgataaac aaggtagatt gagttttccc
    11521 catgaccgag caagacaatg cgttcaggat tgaagcgact aacttgacga cagatttcag
    11581 aaccgattga acctccagct cctgtgacta agatggtctt acctgtcagt tctgcaccca
    11641 gacgcgattc gtcaagacgg atttcctgac gacccaaaag gtccgtaata tcaatttttt
    11701 ggaagccagt acctgcttgg tgaaggccct gaacaacagt ttcaacctta ggcatcttgt
    11761 aacatttgac acccagctta ttacacatct gcaagatacg ctcatattct gacggatcca
    11821 gcgacggaat cgcaacgatg acacgctcga tttgatggcg tttggctaat tcaggcagat
    11881 tgtcataaga gcccaaaaca ggaataccac caagtttttg accctttttc ttagaatcct
    11941 tatccaaaat accgaccagt tctaattcac tggttggatg ttggtaacta tccataaaga
    12001 gagccccacc atcaccggca ccaatcaaga aggttcgacg gtgttctcca tcaccactac
    12061 cttttttgcg tctggagtag attaactgcc aagtaatccg tggcaataaa atcaagaagg
    12121 tactcaacaa gataaagaga atgatgaaac ggatggagaa gagtggcaag aaggcataac
    12181 agatactata tgacaagaca ctgctagcag tcacaccaaa aaagattttc atgaaatccg
    12241 taatcttgct gtaacgacta atgctcgcgt tcaaccccca aaaaccaatc atcaattgat
    12301 agaacaggaa ggccaaactc gtatagataa tgtagtcaac aggtgctgga ttaatcagcc
    12361 cataaaataa aatataagat acaatgatgg aaaccaccat actgaaaata tcaaatattc
    12421 cccagaaaac ctgcttttgt tgtttattta aaatttccac cagatcaatc acataatctg
    12481 ttagtttttt attcatagaa atctactcct ccttaaaaga gttttactat attgttatta
    12541 tagcatattt tgatttattt ggcttatttt atccaaataa attattgttc tcttgtactc
    12601 cttcttccat ttaaatcctc taattaaacc tacaatcgtc ccaaagccat aagcaaagtg
    12661 aatggaaaat aaaagaaagg gcatcacaat tagaaatcca tttttatgtt ttaataaagt
    12721 cagcaaagtg agtaatgaca aaagtagaaa ataggcacct aatagtaaag ttatgaatac
    12781 gaatgtgatc ggtaacaatg ctagactaaa cacaagactc aaaacaaata aacaaggaac
    12841 atagtgaaat aatgataaac acttaaactg aacatgactt gtcaagccaa tccacaaacc
    12901 atttgaatac ttttgatgca gcattttctt gaatgttggt cgaatatact gataagatag
    12961 aatacttggg ctatagcgga ttttataacc atgttctcga attctataat gaatatcatt
    13021 atcttcagtt cggccaagtt gctcatttac taaaccaacc ttctggaaaa cctctcgttt
    13081 atacatccca tgaaaaatag aagaaacata tctatcctcg gaactatttc gataattggc
    13141 aatgctactg ccaaacatat tttcctcaac aagatgcaag gtctctgccc attttccttt
    13201 tccttcgaca atcgtcggtc taggcccccc acagacaaat tcaccttgtt gaataatagc
    13261 cacattgttc attacaaaac tctcagtaac ttttgaatga gcatcaattt ttaaaataag
    13321 gtcccctaca gaatgtttaa ctcccaggtt aaaaccacta gcttgatttt tcttaggatt
    13381 gttatacaat ctaattgagt taaactctgt atcttccttt ataaattgct gaatgatagc
    13441 tgtggtccca tctgtggaca gagcatttat aaatagaatt tcaatatcct ctttaggata
    13501 ggtttgattt tttaagtctt caattagacc aggcagatat ttttcttcat tgtatgcact
    13561 gattacaatg gatacgagaa gactttcaac attttgagtc actactactc tcctgaattt
    13621 ttccttccac cattctatca taattttcaa gactttccca tttttatctt gaaagccaga
    13681 aaccttactt gacattatag tgaaaaagcc ttaaaataag ctgttattaa aatcagctag
    13741 aagaggtatt atatgaaaaa gcaatcactc ttttttgttc caggtattat cctgattggt
    13801 gtttccttgc gaactccttt tactgtttta cccattattt tgggaaatat ttcgcaaggt
    13861 ctggaggtag aagttagttc gcttggtgtc ttgaccagcc tgcctctcct tatgtttacc
    13921 ctcttttcac cattttctac ccaactggct cagaaaatcg gcttggagca tctcttcacc
    13981 tacagcctct tcttcttgac catcggctca cttattcgac taatcaatct gcccctgctc
    14041 tatctaggaa ccttgatggt tggggcaagt gtcgcagtca tcaatgtcct gcttcctagt
    14101 cttatccaag ccaatcaacc aaagaaaatt ggttttctga ccaccttata tgtaacgtct
    14161 atggggattg caacggctct ggcttcctat ctagctgtgc ccattacaca agccagttct
    14221 tggaaaggac ttatccttct cctcacgtta ctctgtctag caactttttt ggtctggctc
    14281 ccaaatcacc gctataatca tagactagct ccacaaacca aacaaaaaag tcaaataaag
    14341 gtcatgcgta ataaacaggt ttgggcaatt attatctttt caggttttca atccttgatc
    14401 ttttacaccg tcatgacctg gttacctacc atgtctatcc atgcaggtct atccagtcac
    14461 gaagctggct tgctgacttc tatcttatct ctgattagca ttcctttttc aatgaccatc
    14521 ccaagcctga caaccagttt atctactcgc aaccgtcagc tcatgctcac tctggtttca
    14581 ctagctggtg tggtcggcat ttccatgctc tttttcccaa tcaataattt catttactgg
    14641 cttgccatcc atctcctcat cggaaccgca accagtgccc tcttccctta tctcatggtc
    14701 aacttttcac tcaagacaag cgcccctgaa aagacagccc aattgtccgg cctatctcaa
    14761 acaggaggct atatcctagc agcctttggg ccaaccctct ttggttacag ttttgacctg
    14821 ttccactctt gggtaccatc tgtagctgcc ctcttgctca tcgatatcct gatgactgtg
    14881 gccctcttta cagtggacag agcggataag atcctttaaa cttctagttt tagctagaag
    14941 ttttttatat ataaaaattt tacacatttc tcttgacagg gctttctttt agatgtacaa
    15001 tgtatgtgta gaaaaattat atataaaaat cttacacatt agaaaaggag gtttcccatg
    15061 tactttccaa catcctctgc cttgattgaa tttctcatct tggctgtact ggagcagggt
    15121 gattcttatg gttatgagat tagccaaacc attaagctga tcgctaatat caaagaatcc
    15181 acactctatc ccattctcaa aaaattggaa ggcaatagct ttctgacaac ctactctaga
    15241 gagttccaag gtcgcatgcg caaatactac tccttgacaa acggtggtat agagcagctc
    15301 ttgaccctaa aagatgaatg ggcactctat acagacacca tcaatggcat catagaaggg
    15361 agtatccgcc atgacaagaa ctgaatacct gactcagcta gaactctatc tcaagaaact
    15421 acctgaagct gaccgtatcg aagccatgga ctatttcaga gagctctttg acgatgctgg
    15481 agtcgaagga gaagaagaac tcatcgctag tttgggaact cccaaagaag cggcccacga
    15541 agttctatcc aatcttctcg ataaaaaaat caatgaagca cccgctcaaa aaaataaccg
    15601 acaaatttta catatcgcct tgttagccct ccttgcagca cctatcggca ttcctctggg
    15661 aatcgccatc ctcgtgaccc tgttcgcaat tcttgtagcc gctttgactg tcattctggc
    15721 tttctttgca gtttccatac tgggtatcat cggcggattc ctatttttag ttgaaagttt
    15781 cactgtcctc gcccaagcca aatcagcctt tatcttgatt tttggttctg gtttactggc
    15841 tatcggtgct tcttcgctag ttttacttgg catttcctat gcaactcgct tcttcggtct
    15901 actcattgtt cgtctggtac aatttgttct taaaaaagga aagagaggta atcagcatgc
    15961 gtaaatggac aaaaggattt ctcatctttg gtgtggtgac taccgttatc ggctttatcc
    16021 tgctttttgt aggtatccaa tctgacggga ttaagagcct actttccatg tccaaagaac
    16081 ctgtctatga tagccgtacg gaaaagctaa cctttggcaa ggaagtcgaa aacctagaaa
    16141 ttactctcca ccaacacacg ctcaccatca cagactcttt cgatgatcaa atccacattt
    16201 cttaccatcc atctctttct gctcaccatg atcttatcac caatcagaac gatagaactc
    16261 tgagtctcac tgataagaaa ctgtctgaaa ctccgtttct ctcttctgga attggtggga
    16321 ttcttcatat cgcaagtagc tactctagtc gttttgaaga agttattctc cgactaccaa
    16381 aagggagaac tctaaaaggg atcaacatct cagccaatcg cggacaaacc accatcataa
    16441 atgctagcct tgaaaatgcg accctcaata caaacagcta tatcctccga attgaaggaa
    16501 gtcgtatcaa aaacagtaaa ctcacaacgc ccaatatcgt taatatcttt gatacagttc
    16561 ttacagatag tcagctagag tcaacagaga atcacttcca cgctgaaaat atccaagtcc
    16621 atggcaaggt tgaactgact gccaaagatt atctcagaat catcctagac cagaaagaaa
    16681 gccaacgaat taactgggac atctcaagca actatggttc tatcttccaa ttcacaagag
    16741 aaaagcctga atcaagaggt acggaattaa gcaaccctta caaaactgaa aaaaccgatg
    16801 tcaaggatca actcattgcg agatctgatg ataatattga tctaatatcc acaccaagca
    16861 gacgttgaca aaaacgctta catttgctac tatggaaaca tatttatcta actaaaaagg
    16921 aggttctacc atgaaacaag aatggtttga aagtaatgat tttgtaaaaa caacaagcaa
    16981 gaacaagcct gaagagcaag ctcaagaggt tgcagacaag gctgaagaaa cgatagccga
    17041 tctcgataca ccaattgaaa aaaatactca gttagaggag gaagtccctc aagctgaagt
    17101 cgaattggaa agccagcaag aagagaaaat tgaagctcct gaagacagtg aagcgagaac
    17161 agaaatagaa gaaaagaagg catctaattc tactgaagaa gagccagacc tttctaaaga
    17221 aacagaaaaa gtcactatag ctgaagagag ccaagaagct cttcctcagc aaaaagcaac
    17281 cacgaaagag ccacttctta tcagtaaatc tttagaaagt ccttatatcc ccgaccaagc
    17341 tccaaaatct agggataaat ggaaagagca agtgcttgat ttttggtctt ggctagtgga
    17401 agcgatcaaa tctcctacaa gtaagttgga aacaagtatc acacacagtt acacagcctt
    17461 tctcttgctc attctgtttt ctgcatcttc ctttttcttt agtatctatc acatcaaaca
    17521 tgcttactat ggacatatag caagcattaa cagtcgcttc cctgagcagc tagctccttt
    17581 aactcttttt tctatcatct ctatcctagt agcgacaaca ctcttcttct tttcattcct
    17641 cttgggtagt ttcgttgtga gacgatttat ccaccaggaa aaggactgga cgctagacaa
    17701 ggttctccaa caatatagtc aactcttggc aattccaatc ttcctcactg ctattgctag
    17761 tttctttgct ttctttgata gcctacgatt tacagccctc ttgtgtgtga ttagcattgg
    17821 aatcattctg cttgccagtc tccatatcat tacaagacca agtcaagcaa gtgaaaccga
    17881 ctccttctat caattattct tgtctgtcct tgtgaacgga gttattatcc tcctcttctt
    17941 tgtagctgaa gtggcactga ttggagatta tcttcgtatc ttggcctttc tttaaacttc
    18001 atactcttca aaaatcaaat tcaaaccacg tcagcttcgc cttaccgtac tcaagtacag
    18061 cctgcggcta gcttcctagt ttgctctttg attttcattg agtagcaatc ctgtcatcct
    18121 tggcaggatt ttttctatgt caaaaaagca agtcgattga cctgcttctg ctttactttt
    18181 cttgtgctaa tgttttaaaa tctttgtcta atatgggttg cacttctatt tgattggcgt
    18241 ctagttggtg gtaaaaagat gggggtaata cctctaagaa ttttgcatag tccaagcgag
    18301 caatggcttc atagtcttct ggtttagagc catctcctgt gatttgaacc aagtcaatct
    18361 cccactggac ttccttatcc accaattgct caatataggc tgcaggaaca aacggttctc
    18421 tcggtaaaac tgtcttgact ccttgagcta gatggtaaac gccatgcact tttccttctc
    18481 catcctgata gaaggaaact ctctcaaagt aggcatctgt ctcttgaacc gcacgcacac
    18541 gcgcttcaaa ctgagccaag ccatctgcct ctaaaaagct tttcaagttc tcacgactaa
    18601 agaaaatagg attaaaaatt ccttggcaaa tcgtaaaacg agctacaggg tctgctagat
    18661 aggcttgaat acttgcttgg aaataagctt caaaatcaaa accatctagc ccttcaatat
    18721 cagtccctgt ataagcaagc aaagcatcta aaaatgactt gataatctgc cagtctgtct
    18781 ttgttagtag gtcaggaaga tcgacacggt aagccttttg accatcagca taactgacct
    18841 taaagagaaa ttgtgattgc cccactatcg cacactcgat atattcgaga cgattaagag
    18901 gctgacggag atagactaca tcatagctat gtgactccaa accgtctagc aaggccaaga
    18961 tcgacttggc attcaaaatc tcttgttctc ctaaaatgct ctgtttattt ggaataaaaa
    19021 atgttttcgc cataactgga ctcctttgaa atcgtttaca tacagtatac cttatttttc
    19081 tgaaagatac aaaaacaaac tttatactct tcgaaaatct cttcaaaccg cgtcagcttc
    19141 gccttgccgt agatatatgt aactgacttc gtcagtctta tctacaacct caaagcagtg
    19201 ctttgagcag cctgcagcta gcttcctagt ttgctctttg attttcattg agtattagat
    19261 ttttgagtac aaagcataga aaaacagccc ctaaggactg tttgatctta tacagtagct
    19321 gcctgatcca agctttcacc gatagcggct aggcgctcaa tcacttcagc ttgtgtcaat
    19381 tcattttttg aaacatagcg gttacgtggg tgaacacggc actcgtgtga gcatccacga
    19441 aggtacttgt cttcattttc ttctgatgtc aagatacgac ggttacagaa gggatttcca
    19501 cagttgacat aacgttcaca tggtgttcca tcaaaccagt ctttccctac gatagttggg
    19561 ttgacatggt tgacatcaac ggcaatacgc tcgtcaaaga cgtacatttt cccatcccaa
    19621 agctcacctt gaacttctgg gtctttaccg taagttgcga ttcctccgtg caattggccg
    19681 acatctttgt agccttcacg gaccatccag cctgagaatt tctcacagcg aacgccacct
    19741 gtacagtaaa ccacgacacg cttgtccatg aatttttcct tgttatcacg gacccattgt
    19801 ggtaactcac ggaagttgcg aatatctggg cgaatagccc cacggaaatg tcctaggtcg
    19861 tactcataat cgttacgtgt gtcaaggaca acggtatctt tatcaagaag cgcttctttg
    19921 aactcttttg gagacaagta agcacctgtt gtttcaagtg ggttgatgtc attgtcaaag
    19981 tcgttgtctt ccaaaccaag gtggacaatt tctttcttgt agcgaacaaa catcttcttg
    20041 aaggcttgtt ctctttcttc gtcaatcttg aaccagagat cttccatacc tgggaggctg
    20101 tgaacgtagt ccatgtattt ttgagttgtt tcatagtcac ctgaaactgt tccgttaatt
    20161 ccctcgtcag cgactaggat acggccttta aggccgattg atttacagaa agccaagtgg
    20221 tctgcagcaa attgctctgc attttcaatt ggagtataaa ggtagtaaag taagacacga
    20281 atatcttttg ccataagatt tgttctcttt tctattctta aattttctaa attttttatt
    20341 tatccatact agtatacctg cttgagaaaa cgaatgcaat ttatttgact ggaaattgta
    20401 gagggttctc gaaatttcct gaatggttaa aataaggaca agagaaaaca tggatatcta
    20461 tatccttgtg ccaaaaaaac cactgccctc cccagaccaa cctgaggaaa gcagtgattc
    20521 ttattttagg agttaggaat gaatacacga aatcaattta gctgattatt ttttgttttt
    20581 caagaattca tcgtattgtt tttgcatttc gttcaatact ttttcgtagg caccttcaga
    20641 tttcaatttt tccatcaatt ctggaatagc tttatctggg tctacagtac cagtgttgat
    20701 agctgtatca aattgttgca ttgtgttagc aatagctgag atttcagatt tcacattgtc
    20761 agtattgaag ataaatccaa gcgctggaga ttctttagct tctgccaatt ctttcttaga
    20821 attttcgatt tgttggtctg taacgttttc gttgatgtaa aggatccagt tgttaccagt
    20881 gttccatcca cccatgtgag tgtttccttt gtagccatca agaacgcgaa cacggttttc
    20941 tttaccttca attttttccc agttcttgcc ttctggaccg taaacaagac cgttcaagag
    21001 ttctggattc gtattcaaga ggttcaagat ttccattgat ttttctttgt tcttagagtt
    21061 gtttgagatg acaaagttag caacttgtgt tgtttggttt ttcttgatga agttagtaat
    21121 tggtttgatt tggatatctt tgttggcaac acgtgaaagc aagctgttac cgtagtcagc
    21181 tggtcctact gtttcttcac gaacgaacca agtatcttgt tgaaggtcaa aggaagtatc
    21241 gcttgttgcg acatcttttg gaatgtagcc agcttcatag aatttgtgaa gagtcttcaa
    21301 gtgttctttg aaacgaggca cttcgtaacg gtttacaact ttagtagtat cgccttcaag
    21361 gtcaataacg aatggaagcc cgtttgctac tgggtagtca aaattatcag atgggatgaa
    21421 aactttacca atagcaaatg gtactacgtc tggagctttt tctttgattt gtttcaagac
    21481 tggctcaaga gtttcgtaag aagtaacacc tgaaatatcg ataccatatt tggcaaggag
    21541 agttccgttg aaggcaaagt tttgagatga tgctacgttg gctgcaactg gtacagcata
    21601 aatcttacca tttacagtat tacccttgat ataagctgga tcaagtgcct tgtaaaggtc
    21661 tttaccttct tttttgtaca attctgtcaa gtcagcataa gcaccttttt gagcatttac
    21721 aatatagtta tctgcaaagg cgatatcata gttttcacca gatgatgtga taactgacat
    21781 tttcttacca tagtcacccc agccaaggta ttggatatcc aatttggcac caactttttc
    21841 ttcgatgatt ttgttggcat ttgctaacaa ttcatccaag ttatctggtt tgtcaccgat
    21901 ttggtacatt ttgataacag gtttgtcacc tgaatcagca gcttttttac tgttacctgt
    21961 caagttgcca caagcagcaa gacctgcagc caaagcgact acactagcag atgcaaaagc
    22021 atattttttc cagtttttca tgataaaaac tccttttttt atttttaaac ttataaacaa
    22081 tgtaatgatc ttatactcaa tgaaaatcaa agagcaaact agaaaactag ccgcaggctg
    22141 tacttgagta cggcaaggcg acgttgacgc ggtttgaatt tgattttcga agagtattaa
    22201 cttcacacaa gggaagttgg gaactgagaa atgttatttc tcaataagca ctattctttc
    22261 acaccaccga tagtcaaacc ttttacaaag tagcgttgga aaaatggata caaaatcgcg
    22321 attggaaggg ttgcaaccac aaccatggcc atacgacctg tttctttcgg tagagcaact
    22381 cccagttgac cagtcaagcc gaccgctttg gcaatgtagt ccatattttg ttggatttgc
    22441 atgagcaaat attgcaatgg atacaagttg tcactcttga tgtaaagaag ggcgttgaac
    22501 cagtcattcc agaaaccaag agctgttaag agcgtgatgg ttgcgatacc tggtagtgac
    22561 aatggcaaac agatttggaa gaaaatccgg gcttcactgg caccatcgat acgagccgat
    22621 tctagaatgg cttctggaat ggtcttcttg aagaaggaac gcatcaagat gatgttaaat
    22681 ggtgagagaa gcattggaac aatcaaggcc caaacagtat ccccaagctg aagcacacga
    22741 gtcaccatga tataacctgg taccaaacca gcgttgaaca acatactgag cagaacgaag
    22801 atggtaaaga atctgcgata cttaaaggtt gtccgtgaaa tagcgtaggc ataggttgtt
    22861 gtgataaaga catttgtcaa tgtcccaact acggttacaa agacagagat gaagagggct
    22921 tgtaggattt tatccttaaa ctgtgccaaa aactcaaaac cgtctaagcc aaattgggat
    22981 gggaagaagc tatagccgta ttggaggagg cttttctcgt ctgtcactga aataatgata
    23041 acgaatacaa aaggtaggat acaagagagg gcaatcaaac ccgaaatgat actgaagaag
    23101 atatctgctt tcttactgaa ggagtgaatg ccgacattat caattttttc ttttttaatt
    23161 ttcttttttg ccatattctc ctcctttcta gaacaaagct gagtttggat cgactcgtct
    23221 tgcaagcaag tttgatagga taaccagaat caaaccaaca acggattggt aaagaccggc
    23281 tgctgcagcc ataccgatat ctgctgtctg agtcaaacca ttaaagacat atacgtccaa
    23341 aacgttggtt acattgtaaa gctgaccagc attgtgtggg atttgataga agagaccaaa
    23401 gtctgcgcgg aagatatttc cgactgcaag gatggtcaat acagttacaa gcggagtcaa
    23461 ctgaggaatg gttacgttgc gaatacgttg ccacttgcta gctccgtcca ctgtcgctgc
    23521 ttcgtagtag gttggatcaa ttcccatgat cgtcgcatag tacatgacac tgctatatcc
    23581 aaagcctttc caaataccta ggaaaagtag gagatagggc cagatgccca ggtcagcgta
    23641 aaaattgact tctttgagac caagactttc caatagatga ttgaacaccc ctttatcaat
    23701 atttaggaag gcatctgtaa agaaactgat gataacccaa gacaagaagt aagggaacaa
    23761 catagaagtt tggaaaatct tcaccattct cttagaacgg agctcgctga ggataatggc
    23821 aatccctaca gatacaacta aacctagaaa gataaagcca agattgtaga ggacagtatt
    23881 tcgtgtgata ataaaggcgt ctcttgaact aaataagaat ctgaaattat cgagtccgac
    23941 ccatttacta tttatgatac tatctatgaa accattactg gtcatgtggt agtctttgaa
    24001 ggcaaccacg ttcccaaata ctggaatgta aaagaataga atcaaccaga gtgtccctgg
    24061 caaaaccatc aagagaaaga tccagttgtc tctcaatgtt tttgaaaact ttttcataat
    24121 ttcctccctt tttattttga tatccatcta aaaattcttt tttagacttt tgataacgat
    24181 tacattatta gtatactcct atttgcaggt taggttaaac tcctaattat agaaaaaact
    24241 ccacaaatta tgtagcagat ttaaaacttt atcaccacta tcaaacaaat gtcctaaatc
    24301 aattgtttat tttatctcta ttagcccagt gatggcgtca ctctgttata agcatccaac
    24361 aacggggtat actgaaaaat ctccagacta gggaactcag agatagttcc taatctggag
    24421 atttttaata tgttattagg cgtttgcttt caacttagca ataacctctt taagattatc
    24481 aatcacctct gcttcagtat gctcagagct ttttcatctg ccaagaacaa aactgctttt
    24541 tgaagttctt tttgagagtt ttcaaggaca tccttatcta ctgtttcaag gtttgagtct
    24601 ttaagaagtt tacttaattc cttggctaat ttcttgagtt tgatttgcag actcatcttc
    24661 tcctgctgtt tctttgcccg ctgtttgtcc tccatcctta gttgctgact ggctttcctt
    24721 aatggactct agggaagcaa tggcatcttt gactgtttgc aaaatatcac gtaaaccttg
    24781 ctctgtcaaa ctatcatctg caaaagcttt cttagcctct gccaaaacca gacgtgctga
    24841 atctgtggta ggattcgata cacctgtcaa tgatctcaaa agattttcta aggtttgagt
    24901 ctgcttacta atactagact aaaatcaaaa agcattatat aacagtgata tgaaatcaac
    24961 taaagaagaa atccaaacta tcaaaacact tttaaaagac tctcgtacag ctaaatatca
    25021 taaacgcctt caaatcgttc tatagtaaaa tgaaacaaga acaggacaaa tcgatcagga
    25081 cagtcaaatc gatttctaac aatgttttag aagtagatgt gtactattct agtttcaatc
    25141 cattatattt cgtctgatgg gcaaatctta ataaagagat tatagagctt ttatagtgat
    25201 ttgaaataag atgtgaacaa ctctatcagg aaagtcaaat taatttatag aaatatttta
    25261 gcagccaagg tgtactgtta taaattcaat acact
//
LOCUS       DAVKQB010000031        23795 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKQB010000031 DAVKQB010000000
VERSION     DAVKQB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.031"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(94..243)
                     /locus_tag="VW528_001301"
                     /pseudo
     CDS             complement(94..243)
                     /locus_tag="VW528_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(397..2028)
                     /gene="rlmD"
                     /locus_tag="VW528_001302"
     CDS             complement(397..2028)
                     /gene="rlmD"
                     /locus_tag="VW528_001302"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9912117.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKIFMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2102..3076)
                     /locus_tag="VW528_001303"
     CDS             complement(2102..3076)
                     /locus_tag="VW528_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9912118.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3150..3764)
                     /gene="pmp23"
                     /locus_tag="VW528_001304"
     CDS             complement(3150..3764)
                     /gene="pmp23"
                     /locus_tag="VW528_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9912119.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3764..4738)
                     /locus_tag="VW528_001305"
     CDS             complement(3764..4738)
                     /locus_tag="VW528_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9912120.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4746..6002)
                     /locus_tag="VW528_001306"
     CDS             complement(4746..6002)
                     /locus_tag="VW528_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9912121.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6064..6495)
                     /locus_tag="VW528_001307"
     CDS             complement(6064..6495)
                     /locus_tag="VW528_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9912122.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSLEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGEGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6497..7099)
                     /locus_tag="VW528_001308"
     CDS             complement(6497..7099)
                     /locus_tag="VW528_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9912123.1"
                     /translation="MIDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7083..7922)
                     /gene="prmC"
                     /locus_tag="VW528_001309"
     CDS             complement(7083..7922)
                     /gene="prmC"
                     /locus_tag="VW528_001309"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9912124.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASNQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     RQD"
     gene            complement(7922..9001)
                     /gene="prfA"
                     /locus_tag="VW528_001310"
     CDS             complement(7922..9001)
                     /gene="prfA"
                     /locus_tag="VW528_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9912125.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLKELNK"
     gene            complement(9011..9490)
                     /locus_tag="VW528_001311"
     CDS             complement(9011..9490)
                     /locus_tag="VW528_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9912126.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDVIIGFVMIAYDKKIVISSGNYLLFCFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            complement(9480..10079)
                     /locus_tag="VW528_001312"
     CDS             complement(9480..10079)
                     /locus_tag="VW528_001312"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9912127.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPIMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            10203..10385
                     /locus_tag="VW528_001313"
     CDS             10203..10385
                     /locus_tag="VW528_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9912128.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10522..11895
                     /gene="mnmE"
                     /locus_tag="VW528_001314"
     CDS             10522..11895
                     /gene="mnmE"
                     /locus_tag="VW528_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9912129.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11942..12877)
                     /gene="dapA"
                     /locus_tag="VW528_001315"
     CDS             complement(11942..12877)
                     /gene="dapA"
                     /locus_tag="VW528_001315"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9912130.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12933..14009)
                     /locus_tag="VW528_001316"
     CDS             complement(12933..14009)
                     /locus_tag="VW528_001316"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9912131.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14351..14423)
                     /locus_tag="VW528_001317"
     tRNA            complement(14351..14423)
                     /locus_tag="VW528_001317"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14388..14390),aa:Thr,seq:ggt)
     gene            complement(14523..15032)
                     /locus_tag="VW528_001318"
     CDS             complement(14523..15032)
                     /locus_tag="VW528_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9912132.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIQKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15030..15126)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15155..15586
                     /locus_tag="VW528_001319"
     CDS             15155..15586
                     /locus_tag="VW528_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9912133.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            15668..16045
                     /gene="mscL"
                     /locus_tag="VW528_001320"
     CDS             15668..16045
                     /gene="mscL"
                     /locus_tag="VW528_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9912134.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16087..17181)
                     /gene="hemH"
                     /locus_tag="VW528_001321"
     CDS             complement(16087..17181)
                     /gene="hemH"
                     /locus_tag="VW528_001321"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9912135.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(17259..18134)
                     /gene="srtG2"
                     /locus_tag="VW528_001322"
     CDS             complement(17259..18134)
                     /gene="srtG2"
                     /locus_tag="VW528_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
                     /protein_id="HEU9912136.1"
                     /translation="MTVQKRARFKNVFLVFFCVFVALFSWQRVVEASDYDHYNPIEKD
                     ASSTGFETLQHLNKDVCGWISLDGTKVDYPLLQSQDNVKYLDRNAFGDYTIMGSIFLD
                     YRFNPNFTDFNTIIYGHSMASGAMFGEIKKFADKEFFDQHRYGSIYYNGRERGLEIFG
                     ILEVDAYDTEIYRTLSSKDEEHQAYYQYLLSKAKYKRDVSLTSTDKIVLLSTCFLNIT
                     NGRHILLAKITDAPVKVAQDESGEAVGTRYFDHGIPSHWIYILAFLLLIIVLLLLVLI
                     ILIIRRDKKTKEQKK"
     gene            complement(18212..19210)
                     /gene="srtG1"
                     /locus_tag="VW528_001323"
     CDS             complement(18212..19210)
                     /gene="srtG1"
                     /locus_tag="VW528_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HEU9912137.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(19228..20424)
                     /gene="pitB"
                     /locus_tag="VW528_001324"
     CDS             complement(19228..20424)
                     /gene="pitB"
                     /locus_tag="VW528_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HEU9912138.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(20469..21020)
                     /gene="sipA"
                     /locus_tag="VW528_001325"
     CDS             complement(20469..21020)
                     /gene="sipA"
                     /locus_tag="VW528_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HEU9912139.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(21020..22780)
                     /locus_tag="VW528_001326"
     CDS             complement(21020..22780)
                     /locus_tag="VW528_001326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912140.1"
                     /translation="MIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYIGSNDSI
                     TSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDVLSKYVQL
                     LPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLEDDAKYVL
                     KFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSRTKIKDYS
                     EKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDSYRKVTSP
                     VQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYRQLPSLTV
                     KKILEGEQSPNKSFTINVTLSDKDGKPINGKFGNTTVTNGKAQISLKNSQETALSYLP
                     RDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVIKKVTGAFANLL
                     QSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKILDLPLNA
                     RYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSSTLVLGVVL
                     PLGGIFFIILLGHLVVNRRK"
     gene            complement(23102..23605)
                     /locus_tag="VW528_001327"
     CDS             complement(23102..23605)
                     /locus_tag="VW528_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEU9912141.1"
                     /translation="MNVQYDFKKIQYFTSSLVIFLAILFLCAPINSLRADSITEPQTT
                     LHKTITPISGQKDQYELSLDITSKLGTETQSEPLDVVLVADLSGSMEERDVWSYSSRR
                     YISRIEALKHTLKGVNGRQGLIDTILSNSQNRLSIVGFAGKIDNQYNDRYYNEYYLSY
                     QYGTWPN"
BASE COUNT         6847 a         4989 c         4447 g         7512 t
ORIGIN      
        1 atttattttt tgagttttaa actttggtag aagtcttgaa ctccttatat ttacataact
       61 atttgcctct ataactgtta tgacacgaaa ccaacaagca agttcaagca cctccatatc
      121 tttattcaaa tgaggactaa gatattcaca aactttatca taaacctctt tatctttttt
      181 cataaaaggg gcatacaact tagcaaattt atcctaaaat tttttgttgc ctttatcaat
      241 catttcaacg ctcctttaaa tataaaaatg atcctatcaa tctttatatt gagcctattt
      301 tctaattgat accttgcaag aaaggaataa tagctgttcc tataccaagt aggaatgtaa
      361 taaatgcaga aagtcatata ctgtcattct tttctattaa atcattttga agtgtctcaa
      421 agcatccatg atggcttctt ctttttcagg tgtacactgt ggaataattt gtttatcctt
      481 tttagacttg ttgtaatgtt ctcgtaattc tattccacat ttctttttta tctgtgcaat
      541 atataatgtc gaaactttta attcaaattt attccaaaca tattctttga tttgagcata
      601 tgttgctttg ctctccgcac ttgtcaaatc catctcatcc agctcaattt caacacttat
      661 gtgcttatcg acatcaagtt tggacaaaag tgctaccgtc tcgacgtgat gcgtttgagg
      721 aaatagatcc accggctgga ctttcttcaa ttcatatccc aattcttggt agagtttgat
      781 atcacgcgcc atggttgcga cattgcagga gatataggcg atgcgatcgg ctcctgtttg
      841 ggcgcttgct ttgataaagc tttctgtcaa gcccttgcgt ggaggatcaa ccaagataac
      901 ggttggttga atcccatctt taagccaatt cttcatagca ttttcagctg tgtcacagac
      961 atagtgggcg tttgaaatat tgttcagctg agcatttttt ttactattct caaccgcttc
     1021 tggaatcagt tcaacaccgt agacttcttt gacatgcttg gcgactgata aaccaatggt
     1081 tccaatacca gaataagcat caataaccac gtcatctttt tttaactctg caaagtcaat
     1141 ggctgtttga tagagtttct ccgccatttc agtattgact tggtaaaagg ctgggccagc
     1201 gatttggaag tcatttccca acatctggtc cgtaatatag tcttgaccat aaagagtgcg
     1261 ccactcctta ccaaaaatcg cattggtatt ctggtcgttg atattttgca tgacagacac
     1321 aatctctggg aactgcttga taacttgttc aatcaattgg tcaacacgaa aaacttttgg
     1381 acgagttgtc accaaaacga ccatgatttg tcctgaatag tgaccacgac gcaccacaag
     1441 attccgaatc aatccagact gttccttttc gtcataaggt tttaaatcaa aacgacggag
     1501 caggtctcga agagctacta cgacttggtc aatgacagga tcctggataa agaaatcttc
     1561 aaggggcatg aggttatgcg aattcttacg gaaaaatcct gtttccaaga caccattcac
     1621 tcgacgaacg ggcacctgcg ccttattgcg atacttgact ggatgttcca taccaagcgt
     1681 ttcagcaact tctacatctg caattccagc aatcttgtag agactgtcct tgacttgctt
     1741 ggttttaaac ttgagctgtt ctggataaga aaggtgtcct aaatccgcga ttcctgaacg
     1801 caggtaagcc aaatctagat cttgattacg gtgtggtgac tggacaaggt atttttcaac
     1861 ttttccaaag ccaatctttt tattgacctt gaggacacgc atgaaaattt tttcactcgg
     1921 taaagcattc tctacaaaaa agaccaaacc atctaccttg gcaactcctg ccccttcatg
     1981 ggtcaaatca acaatttcaa cttctacaat atcatttttc tttaacattc ttttctcttt
     2041 ctattggccg acaaaaaaga cggcaacgtt acggttacca tccattatac catgaaattt
     2101 cttaagaacc aaaactcttg aagaagttga caagggcttg ccagagggag cttaagaagt
     2161 taccaccgtc ctctagcgcc ttatccgcat caaagttaag gttgatattt ttaaaactgt
     2221 cgccagcttg tgatacgatg ctttgtttaa ggtcatttag ggttttagtg aaatctgcat
     2281 tgctgaggat atcactcttt gagagattca aggcaaaatt gatgatgata ttgatctggt
     2341 ttcctgttat gacctgatca agtttgtaat tttttaaggt atcttcaaca atcttgcgga
     2401 tatcttcttc tgtcagattt cccttgcttt ctttagcttt ggcgagtcct gacttgatat
     2461 cagctagggc aacgtttaat ttattagcat catagcctga tttgtccttg ttttcagcat
     2521 tgatatctga caaagccttt agctcttctt gagccaaatc tttattggct tgcggcacct
     2581 tggctccatt agcttctagc gaatagtaga tcccagccaa agcactctca cctgtaactg
     2641 gaatgggtgc tgctacagtg attttggcgt gttccacacc caaagttacg gctgcatttc
     2701 ggtacatatc ctgagttacc ttagtgatgt tttcaggtgt ttcaatcttg acctcaagtg
     2761 gcgatttgtc acctagcttt tgaatcttgg ctgatgaata caactgtaag ctagagtcat
     2821 tggccacatt catgatttta gaataaacat caggtgtcat ggtcttgagt tctttggtat
     2881 ctgttgaggc attgtagccc agttttttaa gagtttgatt tttttggtct tcagataggg
     2941 aagaacctag gacatattca ggttggacat aggtttcatc gataactttt tgaacatctg
     3001 ttgctgcatg gacgctattc atagctgtta ctgcccacaa gatcgcagcg ctagtcagaa
     3061 agagtttctt tctcataggg aatttcctcc tttacttctt tagagtaata tatctatctt
     3121 aaagaaaact tataacaaaa acacctggtc tagccagatg ttgaaaagag agtgaaacat
     3181 ttgatgatgt aaaggttaag tcgtacctgt ctagaataat aatagtttcc tccatttaca
     3241 tagagttcag caccgtgaaa aatggaaatg gggtgaatat aactataagt ctttccagtc
     3301 gtattaccaa gcaagggggc aacagtctca cgagagtact gtttggctag agccaaggta
     3361 ttttccttgc cattttgggc gataaaatcg atataggcag gtccaaaatt ataggcttga
     3421 acagctgtcc agatatctac acccttcttc tgcgccagat agagattgcc tgtcagagtt
     3481 tgaatgcctt gccgaatgct agaggcatta tcattgatgg tgttggtgga accacttgca
     3541 gactcactag actgcataac atcgccttct tttccttttg tttcagtata aatcatagca
     3601 agcacaagct cttcgtttgc tggggtgtct ttttcactca atatttctcg caccatgggt
     3661 tgataggtca tgacttgttt gacatcttga tgaacgcggt aagctttata gccagcaaaa
     3721 aggaagactg ctagtacaag cactcttcga attcgtttaa acattattta ctttggatat
     3781 cctcgatatt tttgattaag atagagtagg ttccattttc gttttggata aactcaacag
     3841 actcggcgtc ttgatagacg ttattgggaa cgatgagctc aattccattt gataaggaga
     3901 gtttttggtt ttcaaatttc tttaattggc gactggcatc aatttcatca aattgaacag
     3961 gttctggtac ggcttctttg acttggtcaa taaagctcaa acgagccgtc agattgttgt
     4021 caaaaaggtc attagccaat ttctcaggtg acaattcatt gctttcttct aggttgttga
     4081 aaatagctga tttgaccttg gattgaaatt gaaaatcatc tgtgttaaaa gattcagcaa
     4141 ttctctgggc tgttttttcc agttccttga tagatttttt aggagaaatc ttaggagcga
     4201 cagcaagaag attatctgaa aaatagttca aaaaagtccc gttgtacttg attcgttttt
     4261 caatcaggtg atacttgcga ctctgaagat tgaccaccaa ggcctcgtca gcacccgttc
     4321 caaatccagg caggttattc tgagtcagct tgattggatt atcaacttct cctccgaggt
     4381 gggtcaaggt ctcccgcagg gcaattcgca agaaagcgaa atgttctaca ccttctttag
     4441 aaaattgaac aaaaatcaag tcattggtct tgagattttc agaaatgcta aactcctctt
     4501 tccagagatt agccagcgtt actgatgtct ccaacaaatc gtctgtaata tgattgaaga
     4561 agggattttc ttcttcgaaa atcccagtct tggcttcatc tgaatacaca tgttcaattt
     4621 ttttacgtag gtattcttcg atttttggag taatattgag aaacttatct gctaagaaca
     4681 gctcggtatc atccggactg aactggtgaa taatggcttt cttaatataa atgtccataa
     4741 aagttttatt cctcgtataa tgggaaggca tctgtcaatt ctttgactgc acttctcact
     4801 tcttctaata cagcctcatt ttctgaattc ttaagggttt taatgatgag ttcagccact
     4861 ttgcgacttt cttcttcacc aaatccacgt gcagtaatgg ctgctgctcc gatacgaatc
     4921 ccacttgtct tgaatggtga caagctttcg taagggattg agtttttatt taaggtaata
     4981 ttgacttcat ccaacaagtt ttgagcaact ttgccgtttt ctacaacttt agtcacatca
     5041 acaaggaaga gatggttttc agttccacct gaaataatac ggaaatcagg gtcttgcaag
     5101 aagacatctg ccatagcctt gctgttctta attacattgg cagcatattc cttgaaggct
     5161 ggatccaaaa cttctttgaa ggaaactgcc ttagccgcca caacatgctc taaaggaccg
     5221 ccctgaatac ctgggaaaat agctgaattg atttttttag caagttcttc gtcattggtc
     5281 aaaatcaaac caccacgagg accacgaagg gttttgtggg tcgttgttgt tgtgatatga
     5341 gcgtatggaa ctgggcttgg atgaaggcca gccgcaacca aaccagcgat atgggccatg
     5401 tccaccatga gcttcgcacc gacagcatct gcgatttcac ggaattttga aaaatcgata
     5461 atttgagaat atgctgaagc accagctaca atcagttttg gttttacttc ttgggcttgt
     5521 ttcaagatag catcaaagtc taagagttcc gtttcaggat caacactata agaaacaaag
     5581 ttgtaggttt gaccagagaa gctaacagga gccccatgag tcaaatgacc acctgatgcc
     5641 aaatccattc ccataaccgt atcacctggc tcaatcaagg acatgtaagc cgcacagtta
     5701 gcttggcttc ctgaatgtgg ttggacattg gcaaatttag caccgaaaat ttcttttgcg
     5761 cgttcaatag caagagtctc tacaacgtct actacatcag ttccaccata ataacggcgt
     5821 cctgggtaac cctcggcata tttatttgtc aagatagacc cttgagctgc cataacagcc
     5881 ttggaaacta cgttttccga agcaattaac tcgatattat tttgttggcg ttcttcttct
     5941 ttggcaatag cattccagag atcagcatca tatgctttaa aatcatcttt gtcaaaaatc
     6001 ataggtcttc tcctttattg tgtgactagt ccattagttt gattttacaa taagaaaatc
     6061 aaactaaaag atgcgaataa accgtttctg cattttatca caagtatagc caactttttc
     6121 ataaaatgca tgagcaccca gacgatgatt ggcagaattt aagcggataa acccataacc
     6181 acatcttttg gcttcttgtt ccaacccttg tagtaaactt ttaccgatac cttcaccttg
     6241 cgcttgaggt gaaactgcta aagctaagat attaaatcct gctttggaat agagtgattc
     6301 gtaaacttca gcgtggacat atccaagtaa gacatgatta gctgcatcct catagccaag
     6361 taggaaatga tgggaatcct gagacagtct agctagttgg ctagccgttt cctctagact
     6421 aaaagtataa cccaaagcct cttggttgat gtcacatatc gctttcacat ctgtttcttg
     6481 caaatctctt agcatttcaa gcctcctcaa aagaaatctc tggcaaccga gcaagaatat
     6541 cttctcgttt aattgcgcct tggcgtaaga ttttcacctt gtctccagac aaatccacaa
     6601 tagttgaatc ctgtccagtt agaaaagcat cgtcttccag acccagaacc tcttggtcaa
     6661 aatccttcag aatttgttca aaggttacac cacttgcctg acctgagata ttggcagacg
     6721 gcccaatcaa gggacctgtc tctcgaatta aatccagtgt gatagggtga ctgggcatcc
     6781 gaaatccaat agttgcaagg tcagaattta cccaataggg aactcggtca ttggcttcga
     6841 gaataatggt caagggacct ggcaaaaagg tctctacaag tttttgtaga taagttggct
     6901 gattcttaga aaagtgcaag atatcctcga aagaggcgat attgagattg agtgccttat
     6961 ctctaggacg acgtttgagt tggtaaacat ggtcaactgc tttttcatct aaggccttgg
     7021 caaaaagacc ataaacagtc tctgtaggta gaacgacagc tccaccattt tccaactctt
     7081 gtctaatcct gtctatcatc aaccacgacc atcctatttt gaccaaattg gtccttgagt
     7141 gttcgaactc ttttttcagg aagatgtttc ctaaaaagtt cgggaacact ttgaccttgc
     7201 ttgtatccaa tttcaaggta aatcttacca ccatctttga gatagtcttt tgcatcttcc
     7261 gcaattctac agtaaatagc taggccatcc tcgtctgcaa agagagctag atgaggctcc
     7321 gaatgcaaaa cattcaagcc tacctctgac tcatcttcac gagagatata gggtggattg
     7381 gatacaatta tatcatattt ttcagaaatt tctgcaaaac aatcagattt tttaaaaaat
     7441 atattaagat tttgattgct agcattctct gatgcaagct ctaaggcctc ttgagaaaca
     7501 tctgctgctg tcactgacca atctggtcta ttttttgcta gagcgagagc aatagctcca
     7561 ctacctgttc caatatccag aactgaaaga ttcgtctcaa gattttcagt tagaataagc
     7621 tccactaact cctctgtttc tggacgagga atcaaaaccc gttcatccac ctttaaatgc
     7681 attccataaa aatctgcctg tccaatgatg tactgagctg gcttgtgagc tgctagttgc
     7741 tggtaaatat cttctacaaa ttgtttttct tcctctgttg tcacctcctg ctggagggca
     7801 aaaataaagt ctgtaaaaga tagatttttc agactacgat agacaaaaga gaggctttcc
     7861 gcttcctctc cttgtcttat caactcttct tcaaaatttg aaaataattg agctaatttc
     7921 attatttgtt taattctttt agtttttgtg tttggtcata aagcaccaag gcatccacaa
     7981 cttcgtccaa tttaccagac aaaatcgtat ctagtttttg gagggtcaag ccgatacggt
     8041 ggtctgtgac acggttttgt gggaagttat aagttcggat ccgttctgaa cggtcaccag
     8101 taccgattgt cgacttacgc tcagcgtctt gttcatcctg agcaatctga gcaaagtggt
     8161 cagcgacgcg tgcacggata atcttcatag ccttctcgcg gttcttctgc tgggtacgtt
     8221 cttcctgcat ctcaaccttg atattggttg gcaagtgaac gatacgaacg gcagtcgcaa
     8281 ccttattgac gttctgtcca ccagcaccag aggcgtgata gatgtcgaca cgaaggtctt
     8341 ttggatcaat gtcgtattca acctcttcaa cttctggcat aacaagaact gtcgctgtcg
     8401 aagtatgaac acggccttgg ctttctgtca caggaacacg ttgcacacgg tgggcacctg
     8461 attcatactt aagcttagag tatacagact gacctgaaac catagcaacc acttctttaa
     8521 aaccaccgac accattcata gaggcttcca tgacttcaaa gcgccaacct tgggcttccg
     8581 catacttttg gtacatagtt agcaaatctc cagcgaaaag tgccgcttcg tctccaccag
     8641 ctgctccacg gatttcaagg atgatattct tgtcatcgtt tggatccttt ggaaggagca
     8701 aaattttcag tttttcttca tattcttctt tttcagcctt ggcatctttg agttcttgct
     8761 tggccatttc ttccaagtcc gcatctccgc ctgattcctt aatcatctct tcggcatcga
     8821 cgatattttg aaggacttgt ttatactcac ggtaggctat tacggtgtca cgattggaag
     8881 cttcttcttt tgaaagctcc ataaaacgct tggtgtctga aacgacatca gggtcactca
     8941 gcaattctcc tagttcttca taacggtctt ctacagcttg tagttgatca tagatgttca
     9001 ttttttctcc ttatttctca attgttaaat catagattgc tactacttca ttctcggata
     9061 tttccccagt ttctttaaat ccataactga gataacaaaa tcttgcctgt tcattttctg
     9121 gttcataaga caaccaaagt ttattgctta aacctgctgg cgctgttcga acatagtcta
     9181 gtactttatc cataattggt ttaaaatatc cttgattttg aaaattctta tcaatcataa
     9241 aacaaaatag taaataattt ccactactaa ttacgatctt tttatcataa gctatcatca
     9301 caaaacctat aattacatca ttatcataaa ctgccaatgg agctacaaaa tctccatttt
     9361 tagtgtagac gtatgcttca gctaaactaa ttgcgttggt tgcaatgaat tgtttttgat
     9421 attccttgac atccaaattt aaaacatcaa aataattttc cattgtaaca tctcttagtt
     9481 caattgtcat agttttgctc cttgttagag gttatcattg gcgcaaaata atgtttacgg
     9541 caaaccgaga tataggtttc attaccacca atctggatct gttctccatc gtaaacgggc
     9601 agtccatcct gtgttcgcaa caccatggtc gcctttttct tgcaatactg acagatggtc
     9661 ttgatttcgt caatcttgtc tgctaaaagc aagagatatt tggaaccttc gaacaattca
     9721 ttgcgaaagt catttttcaa accaaaagcc atgatgggta tgtctaactc gtccacaaca
     9781 cgagctaggt cgtaaacatg gtggcgttta agaaactggg cttcatcgac caaaacacag
     9841 taaggttttt ctggtaggtc tcggatatag ccaaagatat ccgttgtttc ctcaatcgca
     9901 agggcagggc gtttcatgcc aattcgactc gacacatagc caacaccgtc acgcgtatcc
     9961 agagccgagg tcataatgac aacacctttt ccttgctcct catagttata ggccaccttg
    10021 agaatctcga ttgtctttcc agagttcatg gtcccataac gataatacaa ctgtgccatg
    10081 tttcttgctt cacgtccatt tctaaatttt tgctacattc tagtatatca taattttctt
    10141 aagctttaaa cggcaaaatg tggtaaaata gaagaaatca aaaactagtg gaggaagcta
    10201 ttatgccatt tgtacgcatc gatttatttg aaggacgcac gctcgagcaa aagaaagctc
    10261 ttgctaagga agtaacggaa gcggttgttc gcaacactgg agcacctcaa tctgctgtcc
    10321 atgtcatcat caacgacatg ccagaaggaa cttacttccc acaaggggaa atgcgtacca
    10381 aataagctag cttaagcaga attgcttagg ctttttcaat ctccaagtag cattcattga
    10441 agaaatatcc taaatttgtt acaatttgaa aagaaacttg gagaatttcc aagaaaatag
    10501 ctattaatta aaggaaacat tatgattaca cgtgaatttg ataccatcgc tgctatctct
    10561 actccactag gtgaaggggc tattggtatt gtccgcctga gcggaacaga cagttttgct
    10621 attgcgcaaa agatttttaa aggaaaagac ttgaacaagg ttgccagcca cactctcaac
    10681 tacggtcaca ttattgatcc tctgactggt aaagtcatgg acgaggttat ggttggggct
    10741 atgaagtctc caaagacctt cactcgtgag gatattatcg agattaacac ccacggtggt
    10801 attgcggtga ccaatgagat tctccagcta gctatccgtg aaggagctcg gttggcagaa
    10861 cctggtgaat ttaccaaacg tgccttttta aacggtcgcg tagatttgac acaggccgag
    10921 gcggtgatgg atatcatccg cgccaagaca gacaaggcca tgaacatcgc ggtcaaacaa
    10981 cttgatggtt ccctttctga tcttatcaat aatatccgtc aagaaatcct taacacactt
    11041 gcccaagttg aggtcaatat cgactatcct gagtatgacg atgttgagga agccactact
    11101 gctgttgtcc gagagaagac aatggagttt gagcaattac taaccaagct ccttaggaca
    11161 gcacgtcgtg gtaaaatcct tcgtgaagga atttcaacgg ctatcattgg acgtcccaac
    11221 gttgggaaat caagccttct caacaacctc ttgcgtgagg acaaggctat cgtaaccgat
    11281 atcgctggga caacacgaga tgtcatcgaa gagtacgtca acatcaatgg tgtacctctc
    11341 aaattgattg atacagctgg tattcgtgaa acggatgata tagtggaaca aatcggtgtt
    11401 gagcgttcga aaaaagccct caaggaagcc aacttggttc tactagtgct aaatgccagt
    11461 gaaccactga ctgcgcaaga cagacaactt cttgaaatta gccaagatac caatcgcatt
    11521 attctactta ataaaaccga cctgccagaa acgattgaaa cttcgaaact acctgaagac
    11581 gttatccgta tttcagtcct taaaaaccaa aacatcgaca agattgaaga gcgaatcaac
    11641 aacctcttct ttgaaaatgc tggcttgatt gagcaagatg ctacttactt gtcaaacgcc
    11701 cgtcacattt ccctgattga aaaagcagtt gaaagcctac aagccgttaa tcaaggtctt
    11761 gagctgggga tgccagttga tttgcttcaa gttgacttga ctcgtacttg ggaaatcctc
    11821 ggagaaatca ctggggatgc tgctccagat gaactcatca cccaactctt tagccaattc
    11881 tgtttaggaa aataagaaaa atccatgatc cttcattcgg tcatggattt tattgtcttt
    11941 attagtaatc tggtcttaag acccctgtta cagttgcctt agttgcttcg tagtcgccat
    12001 ctacgacaac cttgataatg cgtttggcat cttcttctgg tgctggaaca agaggtagac
    12061 gagtgggtcc agcttcaaat cccatatagt taagaattgc cttaactgga gcaggacttg
    12121 gataagagaa gagagcatta accttaggaa tgaatttacg ctgaattgct gcggctttct
    12181 tcatatcgct ttctgcaatg gcagtaaaca tctcgtgcat ttcatcccca tttgtatgag
    12241 aggcaacaga aataacccca tccgccccaa ggttcatggc atggaaagca tctccatcct
    12301 cacctgtata aatcaagaac tcttcaggct tgtgctcaat caagtaagcc atattagcca
    12361 agctagtaca ttctttgaca ccgataatat ttggatggtc agccaagcga agcatggttt
    12421 ctggagtcaa ttcgacaact acacgccctg gaatgttata gataataatt ggtaggtcag
    12481 aagcatctgc aatagtctta aagtgctgat acatcccttc ttgagaaggt ttgttgtagt
    12541 aaggaacaat agcaagccca gctgcgaaac caccaaattc cgctacttct ttgacaaact
    12601 caatagagtc acgcgtatca ttagtaccta cacccgcaat caaaggaacg cgtccattga
    12661 caaccttttg tacagccgca aacaactcca actcctcatc gtgggtcaaa gttggactct
    12721 cagcagtcgt tcctgcgaga agaattccat ccgtatgatg ggccaataaa tgctcaatca
    12781 aggctggaat agcatcaaag ttaatagaac catcctcatg aaagggggta ataaaggctg
    12841 taatgatttt acattctttt aaatcttgat aagacatgaa aacacctctc tatttcaaag
    12901 aaggagttca tctgaactcc taaacgatat gactatttta attcaaattt caattcggct
    12961 gttggacgaa ccaatccacg ttcatgaaga gtttcagcaa tctgaactga gttccaagca
    13021 gcacctttga gaaggttatc tgaaacaacc cacatgtgaa ttcctttttc tgcatccaag
    13081 tctttacgga tacgaccaac aaaggtatca cgcgaaccaa ctgcattgat agcttgagga
    13141 tagatttgat gagctacatc atcttcaaga acagcacctg ggaaggctgc gatagctgct
    13201 tttacttctt cgattggagc cacttctttt gtttcgatat aaacagactc agagtgagct
    13261 gacaagactg gaatacgcac acatgttgca gatactgcaa tgctatcatc ttccataatt
    13321 ttcttagttt ccttggtcat cttcatctct tcgtacgtgt aatcattatc agtgaaaaca
    13381 tcaatttgtg gaagagcgtt aaaggcgata ggataatgtt tcttgtcacc acctgaaggc
    13441 aaaatttccg catgcaaatc acatggtttc acaccatcat tcaagacttc acgaagttca
    13501 cgttgtgtct caagaattgc tcccatacca gcacctgaaa cggcttgata agttgaaaca
    13561 atgatacggt ccaagcccca tttttggcga accggctcaa gagccaccat catttggatt
    13621 gttgaacaat tagggcaggc aatgattccg ttgtgggcat caagtgcatg agcattgacc
    13681 tctggaacaa ccaaaggaac atctggattt tgacggaaat aagatgtatt atccactact
    13741 accacgccag cttttactgc gtatggtgca tacttagctg atgtagaact acctgctgaa
    13801 aagagagcaa tatcaactcc ttcaaaagct gtttcagtcg tttcttcaat tgtaatatct
    13861 tgatctttaa atttcaatga cttacctgct gaacgtgcag aagcaaggta acggatttta
    13921 tcgattggaa gtgttgattc ttccaacatt tttatcatct gagcaccgac agcacctgtc
    13981 gcgccgacta cagcaactgt atatcccata aataacctct ttcggaattt tctaaaaatt
    14041 tctataatag aagtattata ctactttttc catgaattgc aaagcttttt catcattttt
    14101 tggaaaataa aaatcccagt cgctagacta gggactaaga ggcatcttca tgtgatgagc
    14161 aggttcacac aactcatcaa ggtccgctcc tgcgttatga cctccttatg ctcaatagta
    14221 gtccgaagac tacctatcga ctcatcgcat attctatatt actagagata cttgattttg
    14281 tcaataagaa attttttcga tcgagtataa caaaaaactc tcgtattcaa gagtttccct
    14341 atacatatca tgctaattgc cgggatcgaa ccggcgacct catccttacc atggatgcgc
    14401 tctgccaact gagctaaatc agcttactta agaagtatac tatattcaga aaaacttgtc
    14461 aagtttcctt agaaattttc cataggaaaa agccaggtaa gaactaccta gcttatcttc
    14521 ttctatttac ttaaatcaat tcgacgacca attttaccaa taataatcgc cccaataatc
    14581 aatgaagcaa attcaccgat tcctgttgta aaccaagtaa ggaagaatgg taattctgct
    14641 acaatattta actctgcagc gatagtaaac atggaaattg aaaagaggat tgaaaagaag
    14701 aaatgatctt tttgaatcaa tccgttaaag aggtaatcct tgctatattt tgcaaaaagc
    14761 caaacaccta aactgaggaa tactaaggtt gaaccaccac caacaaagac atctagcagt
    14821 ccgaagctaa agaaattagc aatcatacaa ccgatggtaa ctccgatgat gtacttagga
    14881 ttgtaaaaag ccataaagtt catcatttct gaaatacgaa actgataagc accatagctg
    14941 atggcattta ggggcggtgt gacagtcaaa acgacgtaga tagcagcgac gattgcaata
    15001 tctgcaacat cacgaatagt taattttttc atcttttctc ctttggcggt tgcccgcgtg
    15061 taatatgctt ggtgaaagaa gctaagcacc aagggttgct ttattcaacc ctactagtat
    15121 agcacaatag cctgctttat gctatactta acacatgaaa attcctattc aaaaattttt
    15181 taacaatgaa atcttagcct atctcttttt tggccttgcg acaactctag tttcgattct
    15241 ctcacgacta gtcatttatc aactaagtca tcaggaactc ctggctactg ccctagcaaa
    15301 tattatcggt atcctatttg cctttatcac aaatgatacg attgtattta agcaaaaaaa
    15361 gaaaaataga cttatccgtt tagtgaaatt ttctcttgct cgcctttcta cttttctgtt
    15421 agacttgttt ttaacttttc tctttgtaac ccaatttcct cacattatag ggcaattcgt
    15481 aactgaaaac attgataaga taaatgctat tgaaacaata attgcacaaa tattcattat
    15541 tatcatcaat tatatattga gcaaggccta tatttttaga aaataatgct ccatttatga
    15601 gccttttaat tgccttctat aatgatttag gatacaatga aacacgaaac attgaaagga
    15661 aaacagtatg ttaaaaaatc taaaatcgtt cttgcttcga ggaaatgtta ttgaccttgc
    15721 tgtcggtgtt gtaattgcct ctgcttttgg tgctatcgtt acttcacttg taaacgacat
    15781 tatcactcct cttattttaa atcccgcttt gaaagctgct aaagttgaac gtatcgctca
    15841 actttcttgg catggagtcg gctatggtaa cttcttaagt gctattatca attttatctt
    15901 tgtgggtacc gccctcttct ttattatcaa gggcattgaa aaagcacaga aactgactgg
    15961 cataaagaaa gaaaaaactg ccgaaaaaaa accaaccgaa ttggaagtcc ttcaagaaat
    16021 aaaagctctc cttgagaaaa aataatcgat taaaaggatc tagcgcaaag ctaaatcctt
    16081 ttttctttac tctttgggta acttgcctgc tttttcaagc atttttttga tcagataagg
    16141 catcttaaca ttctcccgcc cttttttctc aatcagtttt ttcacaaatt ctggcatttg
    16201 taaatcttca gattcagcta aaatgttttt cgtctcgtct gaaggaacta actcatcaaa
    16261 ggtctcttct tctgggagaa attccttaaa ctcttgattt tcattgactc taactgtatt
    16321 gaccaaagca tcaatcaaac gagaatctgt atttggcatt ggtggacgat ggtagtttac
    16381 cccaaattcc tggcataagt cataacattc tacatcattg tcaaacaaga cctcaatatg
    16441 ctcactgatg aaactaatag gtacaaaaat ataatggtct ggatgttctg tctgttctct
    16501 gagatactcc aaaacatctg gtttaatcca tggaatacca atatcactct cactctgcca
    16561 agtgttggta tattgttctg aactcaagcc cagtttttca gcgactagct tgctattttc
    16621 gaaaatttgg tctatataag gatcaccaaa gtccaaggca aaaatgggca cactatgggc
    16681 tgaaaagatg actttaaagc tgtcttgctt tacttcttct tttaaaatct tagcaatttc
    16741 atctgcccaa tagtttaaga gcacttcttc ctgataccag tccttaatga ccaaaaattg
    16801 aatttgtttg ctttctaaaa atttttcata gcccatgaca gagtagaaag aataatgggg
    16861 ctccaaaatc aagcagatac attcttcaac accatcagtt tccatctgct tgatcacgtc
    16921 tggaataaag ggacgagaaa atttgttggc aaagtagata ccgtactcct cccctaaacg
    16981 ttctttgact aaattgacct cctcacgtgt aatccgctgc aaaggcgtcc caccgatacg
    17041 aatatagttg tcatagagag tctggatttc gtggtcttgg ggtctaactc cacgacgaat
    17101 gtttgtaaag aattctgcta ctccttcaaa actaatctcc tctggagatc caaaggtcat
    17161 cattaatatg gcttttttca tgttatcatc cttatgatat tattttctct atccattcta
    17221 tcatgaaaga aaaaaatagt aaacgtttcc tacgtctact atttcttttg ctcttttgtt
    17281 tttttatctc gtcttataat taggatgata aggacaagaa gtaagaggac gataataagg
    17341 agtagaaagg ctagaatgta aatccaatga ctgggaattc cgtgatcgaa ataccgtgtt
    17401 cctactgctt ccccactttc atcctgtgct actttcaccg gtgcatccgt tattttagct
    17461 aaaaggatat gtcgtccatt agttatgtta aggaaacagg tgcttaataa aacaatcttg
    17521 tccgttgagg tcaaggaaac atctcgcttg tacttggctt tacttagcag atattgatag
    17581 taagcctggt gttcctcatc cttggaactc aaggttcgat aaatctccgt gtcataggca
    17641 tccacttcta aaatcccaaa aatttcaaga ccacgttctc gaccattgta gtagatagaa
    17701 ccgtagcgat gctggtcgaa gaattcctta tcagcaaatt tcttaatctc accgaacata
    17761 gcccctgaag ccatagagtg tccgtagatg atcgtattaa aatcagtaaa gttgggatta
    17821 aagcgatagt cgagaaaaat tgatcccatt atcgtataat cgccaaaggc attgcggtca
    17881 aggtatttga cattatcctg actttgtaga agcggatagt ctaccttggt cccatcaagg
    17941 ctaatccaac cgcaaacatc tttgttcaag tgctgtaggg tttcaaaacc tgtgctcgaa
    18001 gcatcctttt caataggatt atagtgatca tagtcacttg cttctactac tctctgccaa
    18061 ctaaaaagag ctacaaaaac acagaagaat accagaaata cgtttttaaa tcgcgctctt
    18121 ttttgaaccg tcatcagcac tcctttctat gcgagatttc ttaaaaggaa aggaggcggg
    18181 acaaaaccgc ctcttttcta ttcaccctta gtcagtaaaa gggtctgctt cttgcatttt
    18241 ttttactctc tcttctacga cgtaggatca agtagaggac aaggagtaag aaaatcaaga
    18301 gcaagaacaa tacaaacaag ataaggtacc aaatccatag tgggattgat gagaggaaac
    18361 gtccaagaga cgagtcatca aagacactgt atggaaatgg ttttgatttt tttgtatgga
    18421 aagtattttt agggacggtg tctgtaatct ttgcgactac gatatgacga ccattggtca
    18481 catcgagaaa acaggtactg agtaggatga tacgatcact cggtgagagt gagatatccc
    18541 gcttgtgcat ggcgactgag agcaggtggt ctagataggc ctgctggcgg tcctcaccct
    18601 gtattcctgg atcatagatg ttaaagtcat aggcatcaac ctccaacatc tcaaagatct
    18661 cgagcccttt ttccacacca ttgtagtata tactaccgta acgatgctgg tcaaaaaatt
    18721 cctgatcagc aaacttagcc acatcaccaa acatgacccc attttctacg tggtgcccgt
    18781 agataatggt attaaagtct tcgaacttag gattatttcg tgcatcgaga aagatagcgc
    18841 ctgtcgctgc aaactcacct ttggagtcct tgttgagata cttttcattg tccttggctt
    18901 ggactaaggg atagtcgata ttggtgccat agacattgat ccaaccgagg acttcgggat
    18961 tgagttcttg gagtttgctg aagcctgaaa aactggccag ctcatcctgt tgcgtcgtga
    19021 caggtcgata agcttcgtac tcacttgacg aagctaagga gtagacttgg ttggaatccc
    19081 aaagggcgta gaaaccaaag agaaagatca aagctgcaaa aatgagcagc aagtgattga
    19141 tcaccccatc cacagcaaga acgagacgcc tagacagact cttttttggt ttctcacgct
    19201 tggttttcat catctgcccc cagccgttta ggcttctcta cgttttttag acaagacaac
    19261 aaatccagcc aaaccaagac caatcatcaa aatgaatgga aggttatcaa tcaagagacc
    19321 agtaggagta acgtcgtcga ttttgttagt aactttgttg tcgtttttct tttcacctat
    19381 gaggatatct gttaatgtaa aatctacatt tttttgtcct tgagatgttt gttctgcacc
    19441 atttgtagta taaattgatg aatctgtata acctgctact ccagtttcag ttactgtata
    19501 tttagaacct gctggtagtt gttcaactgt aaatgtttca ccatctttaa gagtaaaatt
    19561 gtactcaact gtatctgctg gggtcactac tgccaacacc tctgacttag atccatgttt
    19621 tactataata ttcgtaacag gggtttctgc tgttttattt gtacttggaa gttgaatctt
    19681 gatatggaat tggaaatctc ttgttttatc ccctgaagct ccatcaactt ttttagtaat
    19741 tgttaatgct ttcgcattgg ggtctacttg actcggatca tcaggattag gctccttact
    19801 tgcgtctttg gtatatgtgt tatcaaataa attataaacg ccttgttcac ttggttctac
    19861 tttaggggcg ttagatttat tattttcctt aaagtatact gatgaaatat agacaccacc
    19921 atttacttta ttcttaacaa taacgtgcat ttcatatgta cgtttgtcgt atctcatggc
    19981 atcaattgtt ttaccatttt tggtaatcgc ctgccatcca gtttttgctt ccgctacatc
    20041 ataaacgtat tcaccagcat ggccataagc aactcccgaa aaaatgtctt ctgttgcttt
    20101 ttgaatctta tcagcagttg ggtgatcatt tttattgtat tctacatttc tatctggaat
    20161 tgcaactccg gtttgatatg gcgcaccgtt agaagattgt cctgtttttt ctgtaaactt
    20221 aaatgtaaat gtcgctgttg gtgttgttat tccctctgca atattcaacg ttttattaat
    20281 cttcacaaca gcattatttt caccatttgc tttggttatt gctgaattat catccgcaaa
    20341 agtcgggaca cctctgaata cggacaaagc ggttatagct gcaatgcttg cagtgaatag
    20401 ctttttaaag aatgtttttt tcatgattat ttcttttgtt tttttatttt cttttttcat
    20461 tatgatcatc aaaagcctcg tcttctaatg actgttacaa ctttgccttg agtatcctgt
    20521 atagggatgg ttccaaaagc acgactgtct acagccgttg ttcgattgtc cccgaggaca
    20581 aaaagttgtc ctgctgggac tttcatcggg aaggttgccc cttccttata gagcagcgtt
    20641 tctttgtaga tatcttgctc ttgttgagga gaaccgttga tgatgagacc attttcgttt
    20701 atatcgattg tacttccttc ggttgctatg acacgcgcca ctctttcctt acctttataa
    20761 ctatagacta gcaaatcacc aatcgaatag cgtttatcca acctataata gacgaccaag
    20821 tcgccatctt ttaaggcggg cttcatgcca tcgtcattgt atcttaataa tccaaagacg
    20881 aaaaagtaaa ggatggctag tacaaatcca accatcagaa gtttactgac taagtaaagg
    20941 atatctcccc agacactcgg cggagactta tcccgattga cttgtgttac tggtttctta
    21001 tgtttctttt taagcagcat cacttcctcc tattcaccac aaggtgacca agtaagatga
    21061 taaagaagat ccctcctaga ggaagaacga ctccaagcac gagagtgcta ctcaagaagt
    21121 caattcccgt ttcaggtacg ctgtttttat tattcgtgac tgtcgcagac ttatttgcat
    21181 caagagttcc ttctttttta tcataggaca cctgataccc acgagacgaa cttgcttctt
    21241 cttcgatact ataacgagca tttagaggaa ggtcgagaat cttgatggtt ttatctttct
    21301 ttagatcaac tgtcgcctta ccattggtga attgtagcgt tgttttttga ttatttacta
    21361 ttgcactata cgatccattc aatggtttat tttgcgcatc ctttacgtta atggtaatct
    21421 taaaggattg cagaagatta gcaaaagcac ctgtaacttt ttttatgact gaaagtgtcg
    21481 gaagtctgtg gttggtgacg attgtttgaa catcctctga caaagtcccc tcttgttttt
    21541 cataggtgac atgatatccc gttctagagt tctctacttc ttccacctta tagtgggtat
    21601 cacgaggcag ataactgagg gcagtttcct gactattttt gagagaaatc tgtgctttcc
    21661 cgttagtcac tgttgtattc ccaaacttgc cgttaatcgg cttgccatcc ttatctgaaa
    21721 gggtaacatt aattgtgaaa gatttattag gtgattgttc accttctagg attttcttta
    21781 cggtgagact tggaagctgt cggtaaataa ctttgaagga tggtttctct ttcgtacctg
    21841 tcttttgaac ttcgactgtg tatcccggag cgtctggtgc tttcagttcg tagtcatatc
    21901 ccttagctac ctttgcaaaa ctagtatttt cagtaaaact attcgtttga actggactag
    21961 tgacctttcg ataggaatcc tttatacttc catctttctt ttggtttaag tttaattcga
    22021 cactaggtgg acgatttgct gatggatttg attttccatc cacacctttc cactcaatct
    22081 ctacaggaac cgtcaatgga tctgacggct tgaaagtggg cttctcagaa tagtctttta
    22141 ttttggtacg tgaggtccca ataccatagg aataggcaac ttttgccccc ttgttggagt
    22201 agagtttagt agcatcggct tcttcggcat catcactagt aagtgtctta tcacccgcaa
    22261 tcgcatcaaa tgcctcttgg ctagaggtga cagtaaactt gagaacgtac ttggcgtcat
    22321 cctctaaagt gtaactcgga ttaaacttgg ctgttacttc cactaatccc tgttcgttct
    22381 tcttagtttc aatcgtaact tgattgtcat tcagttcttg ctcgttacca tccttgattt
    22441 tgacgacacg aaggtgtgaa gcatcaccag gtaacagctg cacgtacttg gacaagacat
    22501 ctgatatagt tacatggtgt acgcctagag gtagaatttt atctgtgatt tctttgaatt
    22561 tttgttgcaa gtcattttca ttattggcag aaaatatttc gttggggatt ccagaattat
    22621 aaccactgat atatccttta agagatgtga tactatcgtt tgaacctata tatctgaatt
    22681 tgattgaata aaagccatct atttttggag ctattaaact agatatattc agcattcctc
    22741 tctctaaacc agaggtaaac caatatggag tatcaatcat acgtcccaca tttggattag
    22801 aataatagtt atatattgtt ctcccactat tagactcgta atacatatta gcttcgccat
    22861 ctgacagcag aataactact ttttttgccc cagcccttgc actttgcaac aatctttgag
    22921 cttcagttaa cccagcattt atatttgtcc cagtgccaat gcccgcgtcc ataccataag
    22981 aacgactaga gtcagcaatt gttaacgaac taacaatatt ttttgagcta ttagaatccg
    23041 tgctccaaga tactaaagtt ttagcatcat catatgaaga gatatttgaa taccaaccag
    23101 ctcaatttgg ccaagttcca tattgataac tcagataata ttcattataa taacggtcat
    23161 tatactgatt atcaatcttt ccggcaaaac caactataga cagacggttt tgggaattag
    23221 aaagaattgt atcaatgagc ccctgacgac cattcacacc tttcagtgta tgttttagtg
    23281 cttcaatcct actaatgtat cgtctactag agtaagacca cacatctcgc tcttccatac
    23341 tccctgaaag atcggcaacc aagactacat ccaagggttc tgactgggtc tccgttccca
    23401 gtttagatgt gatatccagt gacaactcat actggtcttt ttgccctgat atcggagtaa
    23461 tcgttttgtg cagagttgtc tgaggttcag ttattgaatc tgcacgtaaa gaattaattg
    23521 gtgcacacaa aaaaagaata gcgagaaaga taactaaact actggtaaaa tattgaatct
    23581 tcttaaaatc atattgaacg ttcatgctat ctcccttgct agaatatgtt ttaatctgat
    23641 actatataaa tctgagactt ccataagata attgtataat aatattaact tcgtgtcaat
    23701 taatgtgttt gaaaacatgt tattataaat tacaaaaaag tatgattctt ctttgttttt
    23761 caactttttc tgtggtaaaa tggaaaaaat agtaa
//
LOCUS       DAVKQB010000032        23134 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKQB010000032 DAVKQB010000000
VERSION     DAVKQB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.032"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            178..1659
                     /locus_tag="VW528_001328"
     CDS             178..1659
                     /locus_tag="VW528_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9912142.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            2021..3022
                     /locus_tag="VW528_001329"
     CDS             2021..3022
                     /locus_tag="VW528_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9912143.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3173..4090
                     /locus_tag="VW528_001330"
     CDS             3173..4090
                     /locus_tag="VW528_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9912144.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4101..4991
                     /locus_tag="VW528_001331"
     CDS             4101..4991
                     /locus_tag="VW528_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9912145.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            5020..6636
                     /locus_tag="VW528_001332"
     CDS             5020..6636
                     /locus_tag="VW528_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9912146.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6646..7965
                     /locus_tag="VW528_001333"
     CDS             6646..7965
                     /locus_tag="VW528_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9912147.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(8175..8247)
                     /locus_tag="VW528_001334"
     tRNA            complement(8175..8247)
                     /locus_tag="VW528_001334"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8212..8214),aa:Lys,seq:ctt)
     gene            complement(8512..9783)
                     /locus_tag="VW528_001335"
     CDS             complement(8512..9783)
                     /locus_tag="VW528_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9912148.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            10053..10253
                     /locus_tag="VW528_001336"
     CDS             10053..10253
                     /locus_tag="VW528_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912149.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQ"
     gene            10457..10636
                     /locus_tag="VW528_001337"
     CDS             10457..10636
                     /locus_tag="VW528_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912150.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10773..10952
                     /locus_tag="VW528_001338"
     CDS             10773..10952
                     /locus_tag="VW528_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9912151.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            10989..11441
                     /locus_tag="VW528_001339"
     CDS             10989..11441
                     /locus_tag="VW528_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9912152.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11732..12202
                     /locus_tag="VW528_001340"
     CDS             11732..12202
                     /locus_tag="VW528_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9912153.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            12221..13309
                     /locus_tag="VW528_001341"
     CDS             12221..13309
                     /locus_tag="VW528_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9912154.1"
                     /translation="MKKIGTYLVYVLAFVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            13290..13448
                     /locus_tag="VW528_001342"
     CDS             13290..13448
                     /locus_tag="VW528_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HEU9912155.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
     gene            13558..14592
                     /locus_tag="VW528_001343"
     CDS             13558..14592
                     /locus_tag="VW528_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9912156.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            14654..16837
                     /locus_tag="VW528_001344"
     CDS             14654..16837
                     /locus_tag="VW528_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEU9912157.1"
                     /translation="MGPITIPKKTIITLEHLKYLHFSLEETKTYWEKNNIIDGILISI
                     YYNRIVAKSNRINGYFNVGGGNPFPNDTIVGAKFNDEKTKHIVTHYISRDALNKTITV
                     LSKIIEVFEEHFDRAITCEMFSDSSTFASINFSEYGISKSKFQQYLRDSCFIENFGVE
                     HTTVSDIQNSIVTFYDVHTDIFRLLNKLNIDISEANIMNQTTVLLDEKNIELLLSKAP
                     YLVSMIVEDFSKLSVDDFSLDNNDLKINLPSPMNEPVVGVIDTLFDKRVYFNEWVEYH
                     DFVSPDISKDSQDYKHGTAVTSLIVDGANLNPNLDDGCGNFRVRHFGVSLQSGFNSFT
                     IIKQIKEIVSQNADIKVWNLSLGSNDEIRENFISAEGALLDEIQFENDVIFIIAGTNA
                     SVINGKRKRIGAPADSLNSIIVNSVDFNNQSVSYSREGIVLSFFVKPDVSYYGGGNGD
                     FINVCEPLGLGRVAGTSFAAPFIARKMAYLIHIMGLSREEAKALLIDAAIPWNDKKTF
                     TDLSLIGNGIVPIKMDDILSTPDDEIKFIVSDISRAYDTYNYDFPVPISSESYPYVAK
                     ATMCYFPNCSRKQGVDYTNTEMQLTFGRLKSDGIKSINKDNQHAEDTPGYVRENAARN
                     IFRKWDNVKHIGESFTSRKRAKAILNPSNPQWGMSIKTIERLKSGDGQGVRFGVVVTL
                     KELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSLNEEIEFE"
     gene            16888..17904
                     /locus_tag="VW528_001345"
     CDS             16888..17904
                     /locus_tag="VW528_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9912158.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYRDFYEFQNKVYISRTENEEKYSN
                     FIYESLFSDKVNDYENWSDFELSIGKLTKDNDLISSSIEIKEKFIDDFSEVVDDLREY
                     LRIQQEKNLEKGNAIDFISTLDDMRTSLPVINQPAIDKKYNENPHQDDIVNIVTLNYT
                     HVIDKLYNGSAKSFRNQLRANLYNFYIEPPIHAHGTVDVCTVLGVSDEIQISNSLEEQ
                     KESLIKSLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDRYIWSQIAERSISGSV
                     PVIIYHYVPHFDPGNPIRAKRLYRNVEDKFIQNSGIDLELEKKLRDNLIVVIGKTIFD
                     LIER"
     gene            17919..18869
                     /locus_tag="VW528_001346"
     CDS             17919..18869
                     /locus_tag="VW528_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9912159.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTVLA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            18871..19614
                     /locus_tag="VW528_001347"
     CDS             18871..19614
                     /locus_tag="VW528_001347"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9912160.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLTQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            19625..21421
                     /gene="pepF"
                     /locus_tag="VW528_001348"
     CDS             19625..21421
                     /gene="pepF"
                     /locus_tag="VW528_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9912161.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            21668..22222
                     /locus_tag="VW528_001349"
     CDS             21668..22222
                     /locus_tag="VW528_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9912162.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLSNQGL
                     HYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPDFPKNRVHEVFDILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            22333..23001
                     /locus_tag="VW528_001350"
     CDS             22333..23001
                     /locus_tag="VW528_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9912163.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         7341 a         3801 c         4894 g         7098 t
ORIGIN      
        1 tgtattcacg ccctcaatag acggtaaaat aagaaaattg tttatatatt gtctccgtag
       61 tgttattata cgaaataaaa gattttagga aaagtcgtta tatcatgcta tacctattct
      121 ttgtggtata attgcaagag gtttaatccg ataatttata aagagagaaa gatattcatg
      181 agagatttat tatctaaaaa aagtcatagg caattagaat tattagaatt attatttgaa
      241 cataaacgtt ggtttcatcg ttctgaacta gcagagttat taaattgtac agaacgtgca
      301 gtcaaagatg atctatccca tgttaaatct gcttttcctg acttgatttt tcattcttct
      361 actaatggta tacgcattat taataccgat gatagtgata ttgaaatggt ttaccatcat
      421 ttctttaaac attcaactca tttttcgatt ttagaattca tcttctttaa tgaaggttgt
      481 caagctgaga gtatttgtaa agaattttat atcagttcat cttcgctcta tcgtattatt
      541 agccaaatca ataaagtgat taaaaggcaa tttcaatttg aagtcagtct gacccctgtt
      601 caaatcattg gaaatgagag agacattcgt tacttttttg cacaatattt ttcagaaaaa
      661 tattatttcc tagaatggcc atttgaaaat ttttcatcag agccactatc tcaattgtta
      721 gaattggttt ataaggaaac aagctttcca atgaatttgt caacgcatag aatgctaaag
      781 ttgctcctag ttacgaacct atatagaata aagtttggtc attttatgga agtagataaa
      841 gattctttta acgaccaaag tttggatttt ttaatgcagg cagaaggaat agaaggtgtt
      901 gctcagagtt ttgaatcaga atacaatatc tctttagatg aagaagttgt ttgccaatta
      961 tttgtgtctt actttcaaaa aatgtttttc atagatgaaa gtctctttat gaaatgcgta
     1021 aaaaaggata gttatgttga aaaatcttac catctattga gtgattttat tgatcagatt
     1081 tcagtcaagt atcagattga aatggagaat aaggataatc tgatttggca tctgcataat
     1141 accgcacatc tgtatcgtca agagttgttt actgagttta ttttatttga tcaaaaaggg
     1201 aatacaatca ggaactttca aaatattttt ccaaaatttg tttcagatat aaaaaaagag
     1261 ctttctcact atttagagac tcttgaggtt tgttctagtt caatgatggt aaatcatcta
     1321 tcttatacct tcatcactca taccaaacat ttggtgatta atttgctaca aaatcagcct
     1381 aaactaaagg ttttagttat gagtaatttt gatcagtatc atgcaaaatt cgttgcagag
     1441 acactttctt attactgtag caataatttt gaactggaag tttggactga attagaatta
     1501 tcaaaggaat ctttagaaga ttcaccttat gatatcatta tttctaattt tattattcct
     1561 cctattgaaa ataagagact catctattca aataatataa acacggtctc actcatatac
     1621 ttgttaaatg ccatgatgtt tattcgatta gatgagtaac caaaaataac aaaaaaacgc
     1681 tatttcgcgt ttgaactgta cctcaaaagt tagacagaaa aaatctaatt tttggggtgt
     1741 ttttgttaag aaaagacctt caatcattta agcaaaatgt acatctagat ttatagccct
     1801 ctaaaactct tgcttttttt gtagaagata cgaaaaaagc cataaaacca aggcttttcc
     1861 tgttttatgt agatgtttac acatgaaaat tgaagtattt tcaaaagcta attggagtga
     1921 ttttattcat gatgggataa atgatctggc caaaaatgat tgagaaatag ttgcttaaaa
     1981 agcatttgca tgatactata tattgaaagg aggtgataat atggtagaac aaagaaaatc
     2041 aattaccatg aaagatgttg ctttagaagc aggagttagt gttggaactg tttcacgtgt
     2101 aattaataaa gaaaaaggca ttaaagaagt aactttgaaa aaagtggaac aagcgattaa
     2161 aactttgaat tacattccag attactatgc tagaggaatg aaaaaaaatc gaacagaaac
     2221 gattgcaatc attgtaccaa gtatctggca tcccttcttt tcagaatttg ctatgcatgt
     2281 ggaaaatgaa gtctataaga gaaataacaa attactctta tgttctataa atggtacaaa
     2341 tagagagcaa gactatctgg agatgttgcg tcataataaa gttgatggag tggttgccat
     2401 tacctatagt ccaattgaac attacttgac gtcaggaatt ccctttgtta gtattgaccg
     2461 cacatactca gatattgcca ttccttgtgt ttcatcagat aatgatgcag gtggaagaga
     2521 agcagctaaa caattaatta gtaaaggatg tcagcattta gcctttgtgg gcggacataa
     2581 tacaaccatt aatgaaacca aacgtcgtag aatttcattt gaaaagtatg tccaagagca
     2641 taaaatacct cttagtattt ttgatttgga tgagacagtt actgactatt atggaaagct
     2701 agagagtttc ttggaagaaa attcttctat agatggaatt tttacaatca acgattttac
     2761 agctttggat gtgattgaaa tactagaaaa aaatggcaaa cgtattcctc atgatgtcca
     2821 aattattgga tacgatggaa ttaaaatggc tggagataga gattatctac tttcaactat
     2881 tgaacaacca ttggaagaaa tggcaaaaga agcggttcgt attttgtttg atataattga
     2941 tggaaagact gttaatttgc agacaatcct accagtaaaa tttgttgaag gaaaaacaac
     3001 aaaaaatgaa aataagtctt gacagaaagc gctatcaatg atagaatgaa ttcagataaa
     3061 aagatttatt tttaaaacaa aaatgaaacg tttcaaaaaa agaaataaaa agacagcgcc
     3121 aagcgctatc ttttatagaa aaaatgaaac gtttcaaaaa aaggaggttg ctatgaatag
     3181 caaagcgaag caagtttctc tttgggaaag aatcaagaaa caaaaactct tgttattgat
     3241 gactgtcccc ggtttagttt taacctttat ctttaaatac atccctatgt atggggtttt
     3301 aatcgcattt aaagattaca atcctttaaa aggaatttta gggagtgatt ggattggttt
     3361 ttctgagttt acaaaattca tatcctctcc caactttggt atcttgttag ccaacacatt
     3421 aaaattaagt atctatggtt tattgcttgg ctttttacca ccaatcattc ttgcgattat
     3481 gctcaatcaa ctcttgagtg aaaaagtcaa aaaacgaatt cagctcattt tatacgcacc
     3541 aaactttatc tcagtcgttg ttattgtcgg tatgattttc ctcttctttt cagtgggagg
     3601 accaatcaac aattttcttt ctatgtttgg aatgaaggct gacttcttga caaatccaga
     3661 cttctttaga cccttataca tctttagtgg tatctggcaa ggaatgggct gggcttcaac
     3721 gctctacacg gcaacattgg taaatgtaga tccagcctta gtagaagcag ctcgactgga
     3781 tggagccaat atcttccaac gaatctggca cattgatatt ccagctctta agcctattat
     3841 ggttatccaa tttgttttag ctgcaggtgg aattatgaat gtcggatatg aaaaagcatt
     3901 cttgatgcag acatcgttaa atttgccaac ttctgaaatt atctcgacat atgtctataa
     3961 agttggtctt gtatcaggag actattctta ctcaacagcg gttggtttgt ttaatgcagt
     4021 gattaacgta gtattgcttg ttgcagttaa ccaaatcgtt aaacgcatga ataatggtga
     4081 aggaatttaa ggaggaaagt atgaaaaatt cgattatgga tacaaaattt gatagacgta
     4141 tcttactctt aaataaaatc attattgtct ttatcgtttt gatgactttg cttcctttac
     4201 tttatatcgt cgtagcatcc tttatggatc ctaaggttct ggttagtaga gggattagct
     4261 ttaatccagc cgattggact gtagaaggtt accagcgtgt attcagtgac caatctattc
     4321 taagaggttt tatcaattct ctactatact cttttggatt tgcagcttta acagtattgc
     4381 tatctgtgtt tacagcttat cctctttcta agaaagactt ggttggacgt cgttggatta
     4441 actacttctt gattgtgact atgttctttg gtggtggttt agtcccaact tacttgctcg
     4501 taaaagaatt gggaatgctc aatactccat gggctatcat tgttccaggt gctgtcaatg
     4561 tttggaatat tattcttgct agggcctatt tccaaggatt gcctgaagaa ttagttgaag
     4621 ctgctgtcat tgatggagcg aatgacttgc agattttctt taaaatcatg cttcctcttg
     4681 caaaaccaat tatgtttgtt ctcttcctct atgcttttgt aggacagtgg aactcatact
     4741 ttgatgcaat gatttatatc aaggatccaa acttggaacc attgcaactt gtacttcgta
     4801 aaattctcat tcagagccaa ccaggtcaag acatgattgg agcacaagcg gctatgaatg
     4861 aaatgaaacg tttagctgaa ttgattaaat acgcaactat tgtcatttcc agcttgccat
     4921 tgattgttat gtatccattc ttccaaaaat actttgataa aggaattatg gctggttcac
     4981 ttaaaggata aaaaaagaaa aaataaaagg agttttctca tgaaattcaa aacattctca
     5041 aaatcagcag ttttgttgac agctagttta gcagtacttg cagcctgtgg ctcaaaaaat
     5101 acagcttcaa gtccagatta taagttggaa ggtgtaacat tcccgcttca agaaaagaaa
     5161 acattgaagt ttatgacagc cagttcaccg ttatctccta aagacccaaa tgaaaagtta
     5221 attttgcaac gtttggagaa ggaaactggc gttcatattg actggaccaa ctaccaatcc
     5281 gactttgcag aaaaacgtaa cttggatatt tctagtggtg atttaccaga tgctatccac
     5341 aacgacggag cttcagatgt ggacttgatg aactgggcta aaaaaggtgt tattattcca
     5401 gttgaagatt tgattgataa atacatgcca aatcttaaga aaattttgga tgagaaacca
     5461 gagtacaagg ccttgatgac agcacctgat gggcacattt actcattccc atggattgaa
     5521 gagcttggag atggtaaaga gtctattcac agcgtcaacg atatggcttg gattaacaaa
     5581 gattggctta agaaacttgg tcttgaaatg ccaaaaacta ctgatgattt gattaaagtc
     5641 ctagaagctt tcaaaaacgg ggatccaaat ggaaatggag aggctgatga aattccattt
     5701 tcatttatta gtggtaacgg aaacgaagat tttaaattcc tatttgctgc atttggtata
     5761 ggggataacg atgatcattt agtagtagga aatgatggca aagttgactt cacagcagat
     5821 aacgataact ataaagaagg tgtcaaattt atccgtcaat tgcaagaaaa aggcctgatt
     5881 gataaagaag ctttcgaaca tgattggaat agttacattg ctaaaggtca tgatcagaaa
     5941 tttggtgttt actttacatg ggataagaat aatgttactg gaagtaacga aagttatgat
     6001 gttttaccag tacttgctgg accaagtggt caaaaacacg tagctcgtac aaacggtatg
     6061 ggatttgcac gtgacaagat ggttattacc agtgtaaaca aaaacctaga attgacagct
     6121 aaatggattg atgcacaata cgctccactc caatctgtgc aaaataactg gggaacttac
     6181 ggagatgaca aacaacaaaa catctttgaa ttggatcaag cgtcaaatag tctaaaacac
     6241 ttaccactaa acggaactgc accagcagaa cttcgtcaaa agactgaagt aggaggacca
     6301 ctagctatcc tagattcata ctatggtaaa gtaacaacca tgcctgatga tgccaaatgg
     6361 cgtttggatc ttatcaaaga atattatgtt ccttacatga gcaatgacaa taactatcca
     6421 agagtcttta tgacacagga agatttggac aaaattgccc atatcgaagc agatatgaat
     6481 gactatatct accgtaaacg tgctgaatgg attgtaaatg gcaatattga tactgagtgg
     6541 gatgattaca agaaagaact tgaaaaatac ggactttctg attacctcgc tattaaacaa
     6601 aaatactacg accaatacca agcaaacaaa aactagaggt tgattatggg agataagaaa
     6661 tacacagtag aaaaagccaa tcgttttata gcagaaaata aacatctcgt taatactcaa
     6721 tataagcctg aagaacattt ttcagctgag attggttgga tcaatgatcc aaatggattt
     6781 gtctattttc gtggagaata ccatctcttt tatcaattct atccatatga tagtgtttgg
     6841 gggcctatgc actggggaca tgctaaaagt aaggacttgg tgacttggga gcacttgcca
     6901 gtggcacttg ctcctgacca agattatgac cgaaatggtt gtttctcagg ctctgccatt
     6961 gtcaaggatg atcgcctctg gctcatgtac actggacata tcgaagaaga aaccggtgtc
     7021 cgccaagtgc aaaatatggc attttcagat gacgggattc actttgaaaa gatttcccaa
     7081 aatccagttg caactggatc agacttgcca gatgagttga ttgctgctga tttccgtgat
     7141 ccaaaactct ttgaaaaaga tggacgctat tactccgtag tagctgccaa acataaggat
     7201 aatgtgggct gtatcgttct actagggtcc gataacctag tagaatggca gttcgaatcc
     7261 atctttttaa aaggggtaga acaccaaggc tttatgtggg aatgcccaga ttacttcgag
     7321 ttagatggga aagattgcct tattatgtca cccatgcgtt atcagcgtga gggagactca
     7381 tatcataaca tcaactcatc gcttttgttc acgggtaagg tagattgggg agaaaaatgt
     7441 tttatcccag aatcagttca agaaattgat catggccaag acttctatgc gcctcaaaca
     7501 ttgttggacg atcaaaatcg tcgtatcctg attgcttgga tgcagacatg ggggcgtacc
     7561 cttccaaccc atgaccaaga acacaaatgg gcatgtgcca tgactttacc tagaatttta
     7621 agattggaag atggcaaact aagacaattc cctgttaaaa aaggccaata tcaaatccaa
     7681 atagataaag attgtcatta ccacttagga aatgatatag attatcttga atttggttat
     7741 gatagtaatg cgcagcaagt ttacattgat cgtagccatc ttattcaaaa aattctaggt
     7801 gaagaagaac aggacactag tcgacggtat gtagatattg aagctaaaga attggaagtt
     7861 gttctagata aaaattccat cgagattttt gtcaatcaag gtgaagcaag cttgactgca
     7921 acttattact taacggtgcc agctgagcta tcacgaattg attaaaaatt aagttatttc
     7981 tcctaaagaa aaagttctct ttctaaaata gtggaaagag gactttttgt gttttgggta
     8041 tataagctta gtttatggta tttgtaaaat tggtgttgga ttatgattta agctagtttt
     8101 ctaaagaatt tgaaaaaaat tttatttaag caaaaaaaac ccttggttcc aaggcttttc
     8161 ctgttgtatt tatatgcccc ctacagggat cgaacctgtg acccacggat taagagtccg
     8221 ctgctctgcc agctgagcta aggaggcaaa gaaaaaagct gtattggtgc cgaaacttca
     8281 cggtttgtat tgaacccgcg caattaagca ggtgggcaac tcgctctaac tgaagctgtt
     8341 tccgtgtgag acggcctaca tgctgttaga agacttttgt ttccctaata atacaaaaaa
     8401 tagtcggtca acacttaagt gtgaagtcgt acaccacagc gtttctatac ttatatgata
     8461 ccactttttc aaaaaaaatc aagaggaaag tgcaattttt tgaaaaggat ttcagatttt
     8521 tcgcaaacgg tcgatagctt cctttctttg agccaaagcc cgttcatatt taccagtatc
     8581 ttctgcttgg aaatagtgat gattacgaat tttttctggc agatagtctt gcttgaccca
     8641 atttccagga tagttgtgtg gatagagata gtcttgggca ttccccagtt ccttgcttcc
     8701 actgtagtgc ccatcacgta ggtgtcgcgg aataggcaag tgccctgatg ttttgaggtc
     8761 agcaagtgcc ttatccatag ctacataggc tgagttggat tttggagaaa gggccaaatc
     8821 aatcacgaca ttggcaatga gaatgcgggc ttctgggaac ccaatcttct gggcagcatc
     8881 cagagcagtc acggtatgaa tctgggcctc aggattggct aagccgatat cttcataggc
     8941 gataacagtc aagcgacgag cgagactagg cagatcccca gcctcaatca agcgggcagt
     9001 atagtggaga ctggcatcca catctgagcc acgaatagac ttttgcaggg cagatagaac
     9061 atcatagtga ccgtctccat ccttgtccat agtgatgtag cttctctgaa gactattttc
     9121 catgatgtct aaggtgatgt ggcgaatgcc ctcgtcattc tcaggggtag agagaacagc
     9181 taagtccagt gagttaaagg cagagcgaag gtctccgttt gtagaggttg cgatgaaatc
     9241 cagcgcatcc tcatctagtt ctattggaaa atcaaaacca cgttcagggt tacttagagc
     9301 tatctgcagg gcctctttga cgtcttggtt agacagaggt tccaactcga aaatttgaac
     9361 tcggctacga atggcaggag tgacagagaa gaaaggattt tcagtcgttg ctccaatcat
     9421 gatgaccagt ccactttcca agagaggcaa gaggaagtct tgcttggtct tatctagtcg
     9481 atgaatttcg tctagcaata ggacgagacc accagaaaat ttagcttctt ccgagatttc
     9541 ttgcagtcgc tttttactat caactgtcgc attgaaagtt cgaaaggcat acttggtcgt
     9601 tccagcgatg gcagaggcaa tactggtttt gccgattcca ggagggccat atagaatcat
     9661 ggaggacagg cggttggctt ccaccatgcg gcggatgatt tttccaggtc cgaccagatg
     9721 ctcctgaccg atgacctggt cgatggtttt agggcgcatg cgaagcgcga gattgtctgg
     9781 catagcagtc ctttctagcg tggatttgag tagaatgact catctactat atcttactat
     9841 gtccattcta gcctattagt tgtgatattt caatggtttt gtaataatca gggagtgaga
     9901 tagaatcttt tttactattt aatttaatga ggagaggtat cagcatgaga gatttgattt
     9961 tccttaaagc tcaaaatagg atagttgagc ctaaagaagt ggaagtgctg acttgagaaa
    10021 taatattgct gaatgtcatt gtatgaacta taatgaaaag agaggtgata agtaatggaa
    10081 atgatggacc aagtcaggaa attttgattt ttacaaaaca aattcgtcat tggattctta
    10141 gtgatcaagt tatttcaggg aaaagaaaac ttttcttccg ggaagatact ccaaaagaga
    10201 ttttagattt gtacgaaaac attaaatcta aacttgattt tgcttaccaa taagtttatt
    10261 ctaataatgg gttgaaaaaa tatgaaaaat agaataattg atgtttttga agtagtaaat
    10321 cgcatccttg taataactgt tgaaaatcct gatttcgaag acttgagagt taatcatact
    10381 caatgaaaat caaagagcaa actaggaaac tagccgcagg ctgtacttga gtacggtaag
    10441 gcgacgttga cgtggtttga atttgatttt cgaagagtat cagtttgtaa aaattggaga
    10501 taaaaaatat agagtacgca gtgttcctat gattcattca actccgcctc aatctgtctt
    10561 agatcgagat acttttacaa ttgactatac agatgatgaa ttgctcgata aagaagcagt
    10621 ttttactact cattagaaaa ataggtctga ataaattggc ttcccttaga accacatcta
    10681 ggggctttta tgtataatag aataataagt atcactcctt tacaatacgt gtaacacgtg
    10741 ttataatggt gttgtcagga ggtaaaagcg ctatgcctat gacacaaaaa gagatggtta
    10801 aactccttac ggcccatggt tggataaaga ctagaggcgg taaaggctcc catattaaaa
    10861 tggagaagca aggggaaaga cttatcacaa tccctcatgg tgagctgaat aagtacactg
    10921 aaagagggat aagaaagcaa gctgggttgt aaaattcagc ccctgttttt tgatatggag
    10981 gtagtgaaat gttagttacg tatccagctt tattttatta tgacgacaca gacggaacag
    11041 aagcgactta ttttgtccat ttcccagatt ttgaatactc agctacacaa ggagagggga
    11101 tttctgaggc tttggctatg gggtcggagt ggctagggat aactgttgca gatttgatag
    11161 agagtgatgg agagttacct cagccatcag atatcaatag cttgtcttta attgataatg
    11221 atccttttaa agatgatgaa gatttcgtgt caacctatga ccttgataaa tcttttattt
    11281 ctatggtatc tgttgatgta tcagaatacc taggaagtca ggaacccata aaaaagactt
    11341 tgaccatacc aaaatgggca gataagttgg gacgagaaat gggacttaac ttttctcaga
    11401 ctttgacaga cgctattgca gataagaaag ttcaagccta agattaagcg tggtatcgtg
    11461 gtaatagtca ataaaaaagc acgtccactt gtgctagtta ctcgcctatt gattttatag
    11521 atttattagc cctttcgcgg gctttttata ttgattttta tgagaaatga agaaatcaga
    11581 cttttaagaa aatcactgat accaagggtt taaatgaggt aatatggtat aattaggaca
    11641 taaaataatt ttgtggtaag atggtagtat ctattttagc atatttccga gcaatcgggg
    11701 cgattaaaga gtcgcataga aagaggacaa aatggcaaca tacggatttt tagatgtttt
    11761 agaggaagag ttggacaaga actttccctt tgactttgag attagctggg acaaacgcaa
    11821 ccatgcggta gaagtgagtt ttctattgga agcgcaaaat gctgcaggtg tggagatggt
    11881 ggatgaagac ggagaggttt tatcagatga cattcttttt gaggaagcgg tccttttcta
    11941 caatcctgcc aagtcaacag tcaatgagga agactatttg actgttattc cttacctgcc
    12001 taaaaaagga ttttcccgtg aatttttagc ttattttgcg cttttcctta aagatactgc
    12061 tgaggttggg ctagatgtcc tcatggactt tttggaagac ccagaagcag aggaatttgt
    12121 catggaatgg aaccaagaag tctttgaaga aggaaaagtt ggcttggaag agggagaatt
    12181 ttatccttat ccaagatact aggagttggt tggaggtgtt atgaagaaaa ttgggactta
    12241 tttggtttat gtgctagctt ttgtctttat tatgctagct tttgcttgtg gaacaatcgc
    12301 atttgcagag ttggggtatt ccgcagtttt agcctttact tttggttatg ccttcgctct
    12361 tctaagcatg tatttaatct ttattcttca tgagctgggt catgcttttt gtggctactt
    12421 gacaggctat cggctggtgg cttttggatt aggacatttt attttgacca aaaagtcagg
    12481 caggtttcat cttagtagaa cagccattct gaaaaatgtt ggtgctcaat acattggttt
    12541 aaaagaggat gaaagcgatc aaagaatcat cctgatgctt tcaggaggct tgatggttca
    12601 tctcggcttg atattattgg cgatagtgtt tggatttttg acaagaagct ggtattttgc
    12661 agggacttgg atttttctta atttatcttt cttcctaaat aacattttgc cagtcgacat
    12721 caccgatgga gcaaaaattt gggaattact acaacaccct gaaaatacca aatacgccta
    12781 cctggtattg agacattctg ctcaaacttt gctggctcct caagaatatg atttgaaaga
    12841 ctttgttcaa gcagttcctg aggatgcacg agggagtttt gcggatggtg tattaggtat
    12901 gcaaggtgaa gtttccattt tggaagggaa ggaagaagta gctaaacagc agtttcaagc
    12961 attacttgag aggacaacta ctccgatgat gcagacagtt gcccaactat ccctgcttca
    13021 tatcgccttg ttggaaggag attttgagca agcggagcaa tatgctagta ttcgacaggt
    13081 caaatcgttt ctgtctctca aactgtccaa tcttcaagtg atacaagctt ggtatcaatt
    13141 taaggtaaag aaagatgtag atcgaactcg taaggctctc aagattgcta gacagaaaat
    13201 ggatagtagt cgaatgttga gggacgagaa acgctattat gaaaacttgt tagcagagct
    13261 ggaaaaggaa atgttagaag gaactgaaga tggaactaga aatttctgat ttcccaggct
    13321 gtaagattgc tttgttttgt ggggataagc tcttgactat cttacgtgat gataaggcaa
    13381 gtattccctg ggccaatatg tgggaactgc caggtggtgg aaagagtgtc agggaaaatc
    13441 tgatatagtt ttgtattaaa agaatatcct ttcaaggtta ttctttttta attttgtccc
    13501 aaaataattg actttgtccc gaaaatatag tattatttgt gtaaggagga atgaatcatg
    13561 aaaaaatcag atgttcttga tttaataaag tatcattatg agggcagaga gacagaattt
    13621 agaaatcaat ccatagcaat tgctcggaac ttcaataaac atggtgatac acaaatagca
    13681 caatatatta tggggttaat gtctcaaagt gatagattca tgccacaaat agaaaatcct
    13741 agtgagtatt tgactcctgc taaactagat attggtcctc tcccattgcc tctttcaatt
    13801 atgaatgatt taaaaggaat aataaacgct gtcaatcatc atattggaat aaataaattt
    13861 ttatttgtgg gatctccagg tactggaaaa actgaaagtg taaagcaggt ggctagactt
    13921 attggcaagg aattacttgt agtagatttc agtcacttag tagatagtaa gctagggcaa
    13981 acagtcaaaa acttagcaac tctttttaat gaaatcaata atcttccttt caagcaaaat
    14041 tatatcatcc tatttgatga aattgattcc atagtgttgg atagggttaa tcagaatgat
    14101 ttaagagaaa tgggacgagt gacttctgcc tttttaaagg agttggatag gctgtcacca
    14161 gaaattgtat taattgcaac aacaaatctt tttgaaaatc ttgataaagc agttacgaga
    14221 agattcgatg ccataattga ctttgaccgt tatactgatg aagataaagt agaagtagct
    14281 actattattt taaatgagtt attaaagcaa tttaagaatg tagctagaga tttaaaatta
    14341 tttaaaaaaa ttatcaatag tgccaatgta ataccgaatc ctggtgattt gagaaactca
    14401 ataagaacgt cattggcatt tagtgaccca tcagatccgc atgattacca aaaacgcctg
    14461 ctaagaagtt tgcataatgg tagaaattta tctatttcta aattatcgaa gctaggtttt
    14521 actgttagag aaattgagat tttgacaggt atttctaaga gtagtgtatc acgagagtta
    14581 agcgaggatt aaaatgaata cattattgac attacgaggt aaaagtttta cacaaaaatc
    14641 aagaaataat ggtatgggac caataactat acccaaaaaa acaataatta cattggaaca
    14701 tttgaagtat cttcattttt cattagaaga gacgaaaaca tattgggaga aaaataacat
    14761 tatcgatggt attttgatca gtatttacta taatcgaatt gttgccaaaa gtaaccgtat
    14821 taatggctat ttcaatgtag gaggagggaa tccattccct aatgatacta ttgtgggagc
    14881 aaaattcaat gatgagaaga caaaacatat tgttactcat tatatttcta gagatgctct
    14941 taataaaaca ataaccgttt taagtaagat aattgaagtt tttgaggaac attttgatag
    15001 agcgataaca tgtgagatgt tttctgattc atcaacgttt gcttctataa atttttcgga
    15061 gtatggtatt tcaaaaagca aatttcaaca gtatttgaga gattcttgct ttattgagaa
    15121 ttttggagtt gagcacacta ctgtttctga tattcaaaat tctatcgtga cattttatga
    15181 tgtccataca gacattttta gattactgaa taaattaaat attgatattt cagaagctaa
    15241 tatcatgaat cagaccacgg tcttgcttga tgaaaaaaat attgaattgc tactatcaaa
    15301 agcgccctat cttgtctcca tgattgtgga agatttttct aagttatctg tagatgattt
    15361 ttctttagat aataatgatt taaaaataaa tcttccatct ccaatgaacg aaccagttgt
    15421 tggcgttatt gataccttat ttgacaaaag agtttacttt aatgagtggg tagaatatca
    15481 tgattttgtc tcacctgata ttagcaaaga tagccaagat tacaagcatg ggacagctgt
    15541 gacttcttta atcgttgatg gagccaattt gaaccctaat ctggatgatg gttgtggaaa
    15601 ttttagagtg aggcattttg gtgtgtcact acaaagtgga tttaattcat ttacaatcat
    15661 aaaacagata aaagagattg tttctcagaa tgctgatatt aaggtttgga atctttcttt
    15721 agggtccaat gatgaaataa gagaaaattt tatctctgca gagggagcat tattagacga
    15781 aattcaattt gagaatgatg taatttttat catagctggg acaaatgctt cagtaataaa
    15841 tgggaaacgc aaaagaatag gtgctcctgc tgattcactt aattctataa tagtaaattc
    15901 tgttgatttt aataatcaat cagtgtcata ttcacgagag ggtattgttt tatcattctt
    15961 tgttaaacca gatgtgtcat attatggagg aggaaatggt gactttataa atgtttgtga
    16021 acctctagga ttaggtcgtg tagctggaac aagttttgcg gcacctttta ttgcaaggaa
    16081 aatggcctat ctcattcata ttatgggact tagccgagaa gaagcgaagg ctttgctaat
    16141 tgatgcagca ataccctgga atgataagaa aacattcact gatttatctt tgataggaaa
    16201 tggtattgtt ccaataaaaa tggatgatat tttatctaca cctgatgatg aaattaaatt
    16261 tattgtgtca gatatctcta gggcttatga tacctataat tatgattttc cagtacctat
    16321 ttctagtgag agttatccat atgtagctaa agcaacaatg tgttatttcc ctaactgctc
    16381 aagaaaacaa ggggttgatt atacaaatac tgagatgcag cttacttttg gtaggttaaa
    16441 gtcagacggt atcaaatcga taaataaaga caaccagcat gccgaagata ccccaggata
    16501 tgtgagagaa aacgctgcta gaaatatatt taggaaatgg gataatgtca agcatattgg
    16561 agagtcgttt acttctcgaa agagagctaa agcaatttta aatccatcta atcctcagtg
    16621 ggggatgagt attaaaacaa tcgaacgttt gaaaagcggt gatggacaag gagtacgttt
    16681 tggtgtagta gttacattaa aagaattaaa tggggttaat cgtattgaag attttattca
    16741 gcaagctgaa ttacgcggtt ggctagttaa tagattgcaa gtagaagctc aagtagacct
    16801 ctttaatagt ttaaatgaag aaattgaatt tgaatagtgg atattttatc cttattttag
    16861 atcttaggaa cacaagaggg caaagatatg aatattactt atatagttgg aaatggtttg
    16921 gatttgcaat atggtttgaa aacaagatat agggatttct acgaattcca aaataaggtt
    16981 tatataagta ggacagagaa cgaggaaaaa tattcaaatt tcatttatga gtctctattt
    17041 tctgataaag taaatgatta tgaaaattgg tcagattttg aattgtcaat tggtaaattg
    17101 acaaaagata acgacctcat ctcctcaagt atagaaataa aggagaaatt tatagatgat
    17161 tttagtgaag tagttgatga tttaagagaa tatctacgca tacaacaaga aaaaaatctt
    17221 gagaaaggta atgcaattga ctttataagc actctagatg atatgaggac ctctctccct
    17281 gtaattaatc aacctgctat cgataaaaaa tacaatgaga atccacatca ggacgacatt
    17341 gtaaatatag taacacttaa ttatacacat gtaatcgata aattatataa tgggtcagca
    17401 aaatcattca ggaatcaatt acgagctaat ctttataatt tttatattga gccaccaatt
    17461 catgcacatg ggacggtgga tgtctgcacg gttttagggg ttagtgatga aatacaaatt
    17521 tcaaatagtt tagaagaaca aaaggaatcc ctaataaaaa gtttagtttt aaaaaactat
    17581 agagaaaata tggatgtaaa gaatagtgat attataaaaa attctgacat cattattctc
    17641 tatggggttt ctttgggaga aactgatagg tatatctgga gtcagattgc tgaacgatca
    17701 atcagcggtt ctgttcctgt cataatttac cactatgtac ctcattttga tccggggaat
    17761 cccattagag caaaacgcct atatagaaat gtagaagata aattcataca gaatagtggt
    17821 atagacttag agttagagaa aaaattaaga gacaacctta ttgtcgttat tggtaagact
    17881 atttttgatc taatagagag gtaattttaa aaaacaaaat ggaaacatgg caagagttaa
    17941 aagttacagt gaagcgtgag ggagaggagt tggtttccaa tctcttgatt gagctgggag
    18001 cgcaaggtgt tgcgattgaa gacagtatgg actatgtggg aaatgttgac cgctttggcg
    18061 agattttccc agaggtggag cagcaagaag aaatcgtagt gacagcctac taccctgaca
    18121 cggtggatgt gacagtggtt gaggcggact tgcaggctcg cttagcagaa ttgacggatt
    18181 ttatggattt gggagagtta aaaataggta cgactgtctt ggctgaggaa gactgggcag
    18241 ataactggaa gaaatactat gagccagctc gtatcactca tgatttgacc atcgttccct
    18301 cttggacaga ctatgaggcg actgcggggg aaatgattat caagctggat cctggcatgg
    18361 cttttggtac tggaacccac ccaactacca agatgagcct ttttgccttg gaacaggttc
    18421 ttcgtggtgg cgaaacggtg ctagatgtgg ggactggttc aggggttctc tctattgcca
    18481 gctcgcttct gggggctaaa gaaattttcg cctatgacct agatgatgta gcagttcgtg
    18541 ttgctcagga aaatattgaa cttaaccctg gaatggaaaa tatccacgtt gctgctggag
    18601 acttgcttaa gggtgttgag attgaggcag atgtgattgt agctaatatc ttggcggata
    18661 ttctcattca tctgacagac gatgcctatc gtttggtcaa ggatgaaggc tacctgatca
    18721 tgagtggcat tatcaaggac aagtgggaca tggtgcgcga gtcggctgag tcagctggat
    18781 ttttccttga aacccacatg gttcaagggg aatggaatgc ctgtgtcttt aagaaaacca
    18841 aggatatttc tggtgtgatt ggaggctagc atgcagcagt attttgtaaa aggcagtgct
    18901 atctctcctg tcactatcga ggacaaggaa accagtaagc atatgtttca ggttatgcgc
    18961 ttgaaagaag aggatgaggt taccttagtc tttgatgatg gcatcaagcg cttggcgcgc
    19021 gtgctggata tggaaaatcg tcagtttgag ttggtccaag aattaaatga caatgtggaa
    19081 ctaccagttc aagtgaccat cgcatctgga tttcccaagg gagacaagct ggagttcatc
    19141 gctcaaaaag taaccgaact gggtgctagc caaatctggg cctttcctgc agactggtca
    19201 gttgccaagt gggatggcaa gaaattgggt aaaaaagttg aaaaactaga aaaaattgcc
    19261 cttggagcag ccgagcaaag caagcgtaat attgtaccaa gtattaaact tttcgagaaa
    19321 aaagcagatt ttctaactca gctggaccag tttgactcta tcatagtagc ctatgaagaa
    19381 tcagctaaag aaggagaagc cgctgcgctt ctgcaagcag tcactggtct tgaaacagga
    19441 gccaaactgc tctttatctt tggtccagaa ggcggtctct cacctgcaga aatcgaaagt
    19501 tttgaagcta agggagccgt tttggtaggc ctaggtcctc gtattttgcg agcagaaaca
    19561 gcaccgcttt acgccttatc agcccttagt gttttagtag aattagagaa ataagaggaa
    19621 gaaaatggaa caaaaacacc gttcagaatt tccagagaag gaactttggg atttaacagc
    19681 tctataccaa gaccgtgagg atttcttgcg tgcaatcgag aaagctcgcg aagacatcaa
    19741 ccagtttagc cgtgattaca agggcaatct tcacactttt gaggatttcg agaaggcctt
    19801 tgcggaattg gaacagatct acattcagat gagccatatt ggcaactatg cttttatgcc
    19861 tcagacgacg gactatagca atgacgaatt tgcaaatatt gctcaagctg ggatggaatt
    19921 tgaaacagat gccagcgtag ccttgacctt ctttgacgat gccttggtgg tagcagacga
    19981 ggaagtcttg gaccgtttgg gtgaattgcc tcacttgacg gcagctattc gtcaggccaa
    20041 aatcaaaaaa gcccactatc taggggctga tgtggagaag gccttgacca atctcggtga
    20101 agttttctat agtccgcagg acatttatac taagatgcga gctggggatt ttgaaatggc
    20161 tgactttgaa gcccatggca agacctataa aaacagcttt gtcacctatg agaatttcta
    20221 ccaaaatcac gaggatgctg aggttcgtga gaaatccttc cgttccttct cagagggact
    20281 tcgtaagcac caaaatacgg ctgcagcagc ctatctggct caggtcaagt ctgaaaaact
    20341 cttggctgat atgaagggat acgactctgt ctttgattat cttctagctg aacaagaagt
    20401 ggaccgtgtc atgtttgacc gccagattga cctcatcatg aaggactttg caccagtcgc
    20461 tcagagatac ctcaagcatg ttgccaaggt aaatggtctt gaaaagatga cctttgcaga
    20521 ctggaaattg gacttggaca gcgccctcaa tcctgaagtg actattgacg atgcctatga
    20581 tttggtcatg aagtcggtag aacctttggg gcaagaatat tgtcaggaag ttgctcgtta
    20641 ccaagaagag cgctgggtgg actttgctgc taacagtggc aaggattccg gtggttatgc
    20701 ggcggaccca tatcgcgtac acccttatgt actcatgagc tggacaggcc gtttgagcga
    20761 tgtctatacc ttgattcatg aaatcgggca ttctggtcaa ttcatctttt cagacaatca
    20821 ccaaagctat ttcaacgccc acatgtcgac ctattatgtt gaagcaccgt cgaccttcaa
    20881 tgaattgcta ctcagtgatt acttagagaa ccagtctaat gacccacgtc aaaaacgctt
    20941 cgctctggct catcgcttga cagacaccta cttccataat ttcatcaccc acctcttgga
    21001 agccgccttc cagcgtaagg tgtatacatt gattgaagaa ggagaaacct tcggagcaag
    21061 caaactcaac agcattatga aggaagtttt gacagatttt tggggcgatg ccattgaaat
    21121 cgacgacgat gcagctttga cttggatgcg ccaagctcac tactatatgg gcttgtatag
    21181 ttacacttac tctgctggtc ttgtcatctc gacagctggt taccttcatc ttaaaaactc
    21241 agaaactgga gctgaagact ggctcaatct cctcaaatca ggtggtagca agacaccact
    21301 tgagtcagcc atgattatcg gtgctgatat ttcaacagac aaaccactcc gtgataccat
    21361 ccaattcttg tctgacacag ttgaccagat tatcgcctac agtgctgagt tgggggagta
    21421 aggggaatag aaaaattttc tagaatgatg tttatgggct tgttaagcgt cattctattt
    21481 gtcttagttg tagctatttt caaaaatgtc gttgaaggtc gtgatgttaa atttgaactt
    21541 actgtagaac tggtattgat agttatttac gtcattttat atcagattgt atttaattga
    21601 gaattcaata ttgagtcatt taagactgcc tttttgaaca gttgctagaa atgtagaggt
    21661 attgaccatg tatcaagatt tactaagaaa aatagcagaa gaaaaaccaa attataacca
    21721 ggaagaaatc cagtggttgt ttgatcattt gggaaatcct tctccagaaa ttcgtgatga
    21781 cctttcaaat caaggcttgc actatctttc aaaagaaaag gatacaacag gtttctcaag
    21841 tcagtatggt tgggttcacg cttttgcaca tggagccgat ttactgacag aggtggtttg
    21901 tcatccagac tttcctaaaa acagagttca tgaagtattt gatatacttg gccaactatt
    21961 taaaagaatg tcgattcgct ttacagatga tgaggattgg cgtttagcaa gagtgatcta
    22021 tgaacctatt ttacaaggga agttggagca agaacaagta gcttcttgga taaaaactgt
    22081 tgattttccg atagaagaaa gggaggattt ttacaaattt tccaacttta gatcctgtct
    22141 ggtggaagtc tatgtccaac ttgaccagag aaatagttta caagatgagt tgaaagaagc
    22201 tatccagtct tttcaatact agagattagc taatagttct atgattttca aaaaatttcc
    22261 aatcaatttt cttgaaaccc tttcctactt ttgatagact agtatctagt ttttgaaaaa
    22321 aggagaaaga aaatgaaaaa atttgttgct gagttaatcg gtacgttcat gcttgtgttc
    22381 gttgggacag gagctgttgt ttttggaaat ggtcttgatg gccttggtca ccttggaatc
    22441 gcctttgcct ttggtttggc aatcgtggtg gcagcctact caatcggaac tgtttcaggt
    22501 gctcacttga acccggctgt ttcgattgct atgtttgtaa acaaacgttt gtcatcttca
    22561 gaacttgtaa actacatcct tggtcaggtt gttggagctt tcatcgcttc tggcgctgtc
    22621 ttcttcctct tggctaactc aggtatgtca actgctagtc ttggtgaaaa tgccttggca
    22681 aacggtgtca ctgtctttgg tggtttcttg tttgaagtca tcgcaacttt cttgtttgta
    22741 ttggttatca tgactgtgac ttcagaaagc aagggcaatg gcgcgattgc tggtttggta
    22801 atcggtttgt cattgatggc gatgattctt gtcggattga agattactgg actttcagta
    22861 aacccagctc gtagcttggc accagctgtc ttggtaggcg gcgcagccct tcaacaagtt
    22921 tggattttca tccttgcacc aatcgctggt ggagttcttg cagcccttgt tgcaaaaaat
    22981 ttccttggaa cagaagaata attgaaactc aaaaagcctt gctcctcatc ttgaggaaca
    23041 gggctttttc gtatgatact cttcgaaaat ctcttcaaac cacgtcagct tcaccttgcc
    23101 gtaggtatgg ttactgactt cgtcagttct atcc
//
LOCUS       DAVKQB010000033        22830 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKQB010000033 DAVKQB010000000
VERSION     DAVKQB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.033"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            844..1902
                     /locus_tag="VW528_001351"
     CDS             844..1902
                     /locus_tag="VW528_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912164.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALRDLSKQYLNKNISELQDGSFEDGIQDAVASKQIVVINEFIACLNSRCVPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            2216..3193
                     /locus_tag="VW528_001352"
     CDS             2216..3193
                     /locus_tag="VW528_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912165.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKSLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKRDCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            3570..3944
                     /locus_tag="VW528_001353"
     CDS             3570..3944
                     /locus_tag="VW528_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU9912166.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            4152..5054
                     /locus_tag="VW528_001354"
     CDS             4152..5054
                     /locus_tag="VW528_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912167.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWSNKVDKIRIHLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            5110..5463
                     /locus_tag="VW528_001355"
     CDS             5110..5463
                     /locus_tag="VW528_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9912168.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKSDCQGICAALAMSPGT
                     FIALAGSAVLTKKLINYIKFGGLGGWLIGAAAGVLATAAGKIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
     gene            5460..6188
                     /locus_tag="VW528_001356"
     CDS             5460..6188
                     /locus_tag="VW528_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912169.1"
                     /translation="MNNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKAKEYELFKNSK"
     gene            7320..7823
                     /locus_tag="VW528_001357"
     CDS             7320..7823
                     /locus_tag="VW528_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912170.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQDGVFILEDFRNILLYNDVIERISLYEFIGDIFYSKITSCC
                     IVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTD
                     VKEMKWL"
     gene            8110..10155
                     /gene="pspA"
                     /locus_tag="VW528_001358"
     CDS             8110..10155
                     /gene="pspA"
                     /locus_tag="VW528_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEU9912171.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTLVRAEEAPVANQSKAEK
                     DYDAAVKKSEAAKKDYETAKKKAEDAQKKYDEDQKKTEEKAELVRKADEKRQKANLAV
                     QEAYVKFQEAQREFNESPSRKKSDAKKKLDDASAHIEEVKLKQKEADANFNKEQAKVI
                     PEASDLAVTKQKAEEAKKEAEVAKEKYDKAVQEVEVEKNKILEQDAENEKKIDVLQNK
                     VADLEKGIAPYQNKVAELNKEIARLQSDLKDAEENNVEDYIKEGLEQAIADKKAELAT
                     TQQNIDKTQKDLEDAELELEKVLATLDPEGKTQDELDKEAAEDANIEALQNKVADLEN
                     KVAELDKEVTRLQSDLKDAEENNVEDYVKEGLDKALTDKKVELNNTQKALDTAQKALD
                     TALNELGPDGDEEETPAPAPKPEQPAEQPKPAPAPQPAPAPKPEKTDDQQAEEDYARR
                     SEEEYNRLPQQQPPKAEKPAPAPKPEQPVPAPKTGWKQENGMWYFYNTDGSMATGWLQ
                     NNGSWYYLNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGW
                     LQYNDSWYYLNANGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMAT
                     GWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV"
     gene            10555..11676
                     /gene="mnmA"
                     /locus_tag="VW528_001359"
     CDS             10555..11676
                     /gene="mnmA"
                     /locus_tag="VW528_001359"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9912172.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLATAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTNLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            11817..12272
                     /locus_tag="VW528_001360"
     CDS             11817..12272
                     /locus_tag="VW528_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9912173.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNEKTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKIALPDWEGQLRHIHLEE"
     gene            12282..14195
                     /gene="mnmG"
                     /locus_tag="VW528_001361"
     CDS             12282..14195
                     /gene="mnmG"
                     /locus_tag="VW528_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9912174.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            14399..14476
                     /locus_tag="VW528_001362"
                     /pseudo
     CDS             14399..14476
                     /locus_tag="VW528_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(14574..16253)
                     /locus_tag="VW528_001363"
     CDS             complement(14574..16253)
                     /locus_tag="VW528_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9912175.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(16255..16488)
                     /locus_tag="VW528_001364"
     CDS             complement(16255..16488)
                     /locus_tag="VW528_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9912176.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            16768..16983
                     /locus_tag="VW528_001365"
     CDS             16768..16983
                     /locus_tag="VW528_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912177.1"
                     /translation="MYNLFAFAIGVRLLAFIVFVACVYGGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            complement(17306..17458)
                     /gene="cibB"
                     /locus_tag="VW528_001366"
     CDS             complement(17306..17458)
                     /gene="cibB"
                     /locus_tag="VW528_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9912178.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(17460..17645)
                     /gene="cibA"
                     /locus_tag="VW528_001367"
     CDS             complement(17460..17645)
                     /gene="cibA"
                     /locus_tag="VW528_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9912179.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            17823..18506
                     /gene="tsaB"
                     /locus_tag="VW528_001368"
     CDS             17823..18506
                     /gene="tsaB"
                     /locus_tag="VW528_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9912180.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            18503..18940
                     /gene="rimI"
                     /locus_tag="VW528_001369"
     CDS             18503..18940
                     /gene="rimI"
                     /locus_tag="VW528_001369"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9912181.1"
                     /translation="MIEIKRIQQHPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            18930..19940
                     /gene="tsaD"
                     /locus_tag="VW528_001370"
     CDS             18930..19940
                     /gene="tsaD"
                     /locus_tag="VW528_001370"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9912182.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHELPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFANLDLNAKPSLAFDT
                     ME"
     gene            complement(19980..21286)
                     /locus_tag="VW528_001371"
                     /pseudo
     CDS             complement(19980..21286)
                     /locus_tag="VW528_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(21678..21758)
                     /locus_tag="VW528_001372"
     CDS             complement(21678..21758)
                     /locus_tag="VW528_001372"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9912183.1"
                     /translation="MLPLPTYSPEYNPIEKIWAYIKNISE"
     gene            complement(21959..22285)
                     /locus_tag="VW528_001373"
     CDS             complement(21959..22285)
                     /locus_tag="VW528_001373"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912184.1"
                     /translation="MEKVDRDKLKIYIETHPDAYLTEIAAEFNCPPTTIHYALKAMGY
                     SLKKSRTYCEQDPEKVNRFLKELNHLSYLTPIYIYETGVKTYFYLEYDRALSRQLVSL
                     EEDIII"
     gene            22522..>22830
                     /locus_tag="VW528_001374"
     CDS             22522..>22830
                     /locus_tag="VW528_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU9912185.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     L"
BASE COUNT         7119 a         3888 c         4834 g         6989 t
ORIGIN      
        1 ttattgaaga tataaattaa aaacgaggtt gggaaaaaac tcaatttgaa agtgagattg
       61 agtaaatctt tctatctata taacgtatat catcaatatt tttgaacacc tgataatatg
      121 cacttttctt attttaaaga ttttttgcct ttattcattt cttgaatgaa ttggagagat
      181 gactttattt aggtgtttga cattactata gataggattt agcagtacta atatgaactc
      241 atattatagg tgtagactta aattttggaa gcaagctaaa ggatatctta tctaggtaac
      301 taagtgctat aacaagatat attggtggag ttagtatggc aactattact aatgctctta
      361 atattgtggc tactgttgct gaagtcttct cattaggagg agccattgca tatggtttag
      421 atatcgtaga cggtaaattt gatgggtatt tatgggcgta aaactatagc tatgaaacat
      481 ttttttgcag gaattggtga aattcatttt ttgttatttt tattatatct aagcatgtct
      541 gctagtccaa ttattggact agtgattgga tatactaaaa taaatctaat aaattctatt
      601 attatagctc ttggtatgat attggcatta attttggtat tatcaagagt gtatagaaaa
      661 tatttttata gagacgatga tgactaaagt ttttatcaat aatttgggct ccttgtcaac
      721 tgtagtgggt tgaataaaag ctaacgtctg gggaagggtt taaagctttg ctttatcaaa
      781 tattgactta cagagttgct ctattgtatt gatgcattct tagtaaataa tatggtgttt
      841 taaatgaaag atgatcagaa atatttatta gcgggacttt attcgcttct agttgcaata
      901 ttttattttc cattgattga ttccaagggg atttttgtaa gtatattgat ggcggtttta
      961 cttttatatt taatatactt cattgcaaca gtcattcata ttgtgataat taagtttata
     1021 agaaaaaaat cttttaagta tttggttcta tatccattta cttatgatgg gagctggaga
     1081 tttcaaccta taaacttact ctactttcca gaaatggtga gagatgtcat tccgattaat
     1141 ttagttcaag aatattgcca aggacaacct tacggactgt taaaaaagat gctgaaaaga
     1201 attcgattgt cacgagagat atctttactc ctggctacca ttatcgtcta tttttttacg
     1261 catagaattc tacctctttc agtatttacg ttcatatttt cttacatatt gctatttgca
     1321 caatcttatt tgggtggtaa taccgcttgg attggaaata gaagattaat aatagatgat
     1381 gaatttgaaa aaatcttgct ttcaaaaagt tatataaaag aaatatcatc tgcacgatac
     1441 tcagaatact tgacatgtga atacaaaaac tttacaccaa tcatattgtt agctattttt
     1501 gaaaatttgc ttgactccta cttgcttcag aatcagagtg aggttgactt agatattttt
     1561 tataaagttt tacctttgct ttataaagaa aaatatacta tgggattcaa ttactttgta
     1621 agtttaaatt atttactata taaggttgga tttttgggaa tcatttacga taatgaagca
     1681 ttaagagatt tgtcaaaaca atatttaaac aaaaatattt cggaattaca agatggttct
     1741 ttcgaggacg gtatacaaga tgctgttgcc tctaaacaaa tagtagttat aaatgaattt
     1801 atagcttgtt taaattccag atgcgtgcca tcccaatatg atagattttt ttataaagat
     1861 agaccttata ttttttccag aaagagtcct attaaggggt gaaaatttgt tacatttgga
     1921 ttaacaagtg tcctagggat tagaaataac tctagtatag ctagatattt ttgagtaact
     1981 agaaatatca ttagtctacc catatgattg ccagaaaaga tggctgatga atcttattta
     2041 tactgttgca gtaggcgttg tctaggataa ataagctatc ttgatggggg agattggaat
     2101 aattttagta gaaacattaa aggattatgg acaagtagcg caggattagg tgatgcaatg
     2161 tgctaagttg gcttggtgtt agcagtagta ttatttaaca atggaaagta gactaatgaa
     2221 aaataagcgt tatttttttg acacaatttt aataatttta ctgcttataa gcacgatatt
     2281 ttgtgtcagc ccagtattta taaaattgga catcttagga actccctctc atgccatctt
     2341 aacctttgta ttggcgatac cgttattcta tattttgtct caatgcttac atactttgct
     2401 attactagtt tcctcaatct tttgtaaatt aagaccaatt tatttttatt ttatatttgt
     2461 gatcattata ggagctagga aatactatcg aattctcttt caccaactaa tgggatttag
     2521 tccaggaata gcagtgtttt ataaggagag tcaaacaact aagaatctgt ttaaatttta
     2581 ctacttcttg tactttacaa cactaatttc atactatttt ttctttactt ttgtgtatga
     2641 caaatcgttg cttttgcctc ttattccatt ttctattatc atagcattag tacaaaaact
     2701 ttatagaatt gagaatcaac aattatttct gttaaaatca aaagtactaa caatactgga
     2761 atctaagagg gattgtgagt ttaatcttca ggattatcat gaaatttgga aattgcaatc
     2821 aaaatctgag ttgccatgcg tggctttatc ttatatcagt ttgatcaagc catatctttc
     2881 agagagtgtg cgtgaacaaa ttgatttatt agaagttaag cgatttaaaa aaataaatca
     2941 tccaatatca ctttatggta tgttggatgt tataaaatta aatctatatt tacggcatta
     3001 taatgaaaaa aataaatatg aatcaatgtt aaagaaaatt ttagaggtaa gaccagactt
     3061 tgttttaatc gagcagaata ttgatgatag tttaaatagc tcgcaaccct tatctttatc
     3121 tttagccatt tcagaaatcc aacttttgct tgaagtgtat atggggatca aacatgtttc
     3181 aattaggaga taaaaccaga ataatatttg ccattatagg ggcaagttgc atattagtca
     3241 gtatgtatct tattccactt attaaaactg agaataaatt ttgggaaata cttttattta
     3301 tattaggtgt attgctaatt ggaattgcgt tactaagtca aaaaaaataa attttgaaat
     3361 agtattagtg agttcgcaat tatttattgc tagtggttta gaactgttaa gtttcaaaca
     3421 ataattgatt ttaaacctta ggtaacctat aaaaacaaat aaggtagaga aaggatatct
     3481 tatataggta gctaagtgat aaaaacacag tcgtctatca cttgttagga aactatccta
     3541 actatgctta ataaaaaagg agaaaataaa tggaactcgt attaccaaat aattatgttg
     3601 ctcttgagca agaagagatg atgtatcttg atgggggatt ttctattctg agatggcctg
     3661 ttgcaacagc cattaatata gcttttaatg gtgttttagg tggaggagca atcagtctag
     3721 ttagaaatta tattcgtaat tatggtttgc ggcgagttac aagcgcaatt gctggagcag
     3781 ctgcaagata tgttggggta cgagttgcaa atagagtggc aggatttgca ctgtctgcta
     3841 ttaatggatt tgcagcttgg atgtcaattg gcgatgctat tacaacaatc tgggccaaca
     3901 atgatgtaaa taggagagac ccaaatttaa acgccttgtg gtaactagat atgggtatta
     3961 aaatggaaaa aatttttgtt attatttttt ttgtatgtct atttatatca tccataactt
     4021 ttttagccta tgattttgtt agcgaagaaa taaaaaaatt gattatttgg atgaatgtcg
     4081 tttttttgat tttaatcata gcaatgataa tttatccaaa attaagaaaa tgataaggac
     4141 actgtaaaat tatgatacgt aaactaatca tttttttatt aatgctccct ctttttgtct
     4201 tgtggctaga gttgcatgtc ctagtgaata acttactatt gaatctagaa tctccccttg
     4261 attttgtgat tagtatgagt cttgctttct gtagtttgat tttatcaaaa atagttttag
     4321 acatactata cgcattgaaa gatttgtaca agaaagaagc tcttattacg atttttcctt
     4381 ttatttttat aggcaggaag aaagtaaatg tgaggttctc gccctatttt tcattccatc
     4441 gtaaaagttt gtcgcctgat gatttaagat ctaggattat atggagtttt attcttgaaa
     4501 ttgctattat cctagtattt attttaaaaa ttccttttgc aatcatcatg cttactacaa
     4561 tatttttctg gactataatg gatatcaatc atctcgtttt taataagacg gaatttcttt
     4621 tcaatcaaaa taaatgggaa aaagaagatt cgtttgaaag tgatttgaca aatacgttaa
     4681 aggataaaat acaaaaatca gatctcagtt attctgactt aatgtctctt cagctgtatg
     4741 atgcgatgaa tcaatctacc tttttaacgg atagtgaact ctttgaggat attctgagta
     4801 aaattgaaga ttctcataac acccttctgt gtacaggtct tgtcgagtta ctgctgtatg
     4861 agatttctat tagtaacaat aacaactggt ccaacaaggt tgataaaatt aggatacacc
     4921 tcatcagaat caatcaatta gattttttct attatacaag ttggttaagg caaaattttg
     4981 atttctgtat gaatagagag tatcataaaa tgaaatctcg gaaattatta ctgagtaata
     5041 aaaagatagt ttaagttcgt cctatttgta aaacaaatga aacaatttca actaaggagg
     5101 agaaaacaaa tggaacttgt attaccaaat aattatgttg tgattgatga agaagaaatg
     5161 atgtatcttg atgggggggc ttatttaagc aagagtgatt gtcaaggaat ttgtgcagct
     5221 ttagctatga gcccaggaac ttttatagca ttagctggat ctgcagtttt aactaaaaaa
     5281 ctaataaact atattaagtt tggaggtctt ggaggctggc ttattggtgc agcagcaggt
     5341 gtattggcta cagcagcagg aaaaatagct tactgtattg gatatggtgc tcttaataga
     5401 ggttgtgata ttagcgggaa cccttatcct tgggatggat tcatatctgc gacagtaaga
     5461 tgaataatgt agataagatt cgaaaaatac atattatcgt atgttggatg tatatatttt
     5521 tatcattcag ggcgattata aatgacacgg agtatttttt gcttattttt ttagctttta
     5581 tctactcaat agtatcacta cctctatatt ctgtgaaaaa taaaatagta tctatctgtc
     5641 tagccataaa ttcgattctg ttaatgagtt tccccatttt aatcaataag tttttcccag
     5701 aaagtttttt gacttatacc gtattaataa gtgtttttat cctagagtta gcaatctttc
     5761 atttaattgg ggaagatttt gctactaaat tgactaacga atataagaaa attagtcagt
     5821 ttagaagcaa ggtgtctcaa tctccttgga taaaatattt agagatttct agttttatat
     5881 taactatatt tccatctatt ctttacggta cagttgataa tcgtgtattg gctcttatct
     5941 ttttgataaa aatttgtgta gatactacga taaaattttt attcatcaga ttatttgaca
     6001 caagtacttt aatgaagagg agaatatttt ttcttttcgc attggatgtt atagcttact
     6061 tatttttagg atatctttta gtgattcaaa aagcaggcta tttgttttcg gttttacttc
     6121 ttttttctaa tttttcagtt ccatttatca aagcaaagga atacgaatta tttaaaaata
     6181 gcaagtaata gaaacatagg ttaaatctta gaacggctta aaatcagata tgataaaggg
     6241 aaatagtctt atctaggtaa ctaagtgcta caaacacagt cgaggatcac ttgttaggat
     6301 tacattttaa tcatgcttaa taagaatgga gaattcctat ggggattgta ttactctgaa
     6361 gtttcgaata ccttagttct cgtgattttg agctgttcta gctttagaat ccttcagaat
     6421 gtaaaatttt aagcagtcaa agacttagaa tagcctggaa acatttattt tatagcaatt
     6481 ctaggcttag aattgctcat atatatgtat ttgagctgct cgatagttag gacagctcta
     6541 ttctgagaaa tctgagctat caatgcttca gaatagtatt tcgcaaatgg tttttattga
     6601 agaggtgatt gttttatgat agtctaaaat aaaactgatt taatggagta aatggagaat
     6661 cgtcacatga aacatttttt tgcaggaatt ggtgagataa aacttgttgg ctacttattg
     6721 tatatatgtg taggactagc ccctcttttt catatatatg tcataggttc ggaactgagt
     6781 tttgtgaaaa tagtgttatc tattttggga gttatatttg taagtatgtt aactattgct
     6841 cgaatttatc ggacttttct ataaagaata gtttggactt gaactgtatc taaaaacaga
     6901 ggttggtcga ttatcagcct ctttcttgtc gttgaaaaga gtgagatata ttgacttttg
     6961 aaaaaaatgt cataattgag ttgggataaa cctaaaagta atatagtaga ttgaatctgg
     7021 aatagtccgt tatgactgtt aaaatatttc tagaaattaa tttgactttc ctaatcgatt
     7081 tgttcacatc ttgtttcaat ctactatact ttctcgttaa accgttcggc aattcatggt
     7141 gacatggaca catagtagta agcacattat gtggcttggc agagctaaaa actgttccct
     7201 tgcgataagc ctaataagca caacataggg agttagagaa gccgactcta atcaaccact
     7261 ttgggcagta gtgagaactg ccccgtgctt tttattttga gaaaatatgg agtttgtcgt
     7321 tgaaattact tgattgtatt ttggactatc aagaaagatt caatggaaaa acatgtcaag
     7381 tatcaacgaa ttataagtat ttagagactt tcaaagtgaa tttttgcttg actgatttac
     7441 atcatttatt tggcttacac aaaatcacac gagattatgc tagtcaaaca aaacctgcta
     7501 ttcaagatgg tgtttttatt ttagaagatt ttagaaatat cctattgtac aatgatgtaa
     7561 tcgaaaggat atctttatat gagtttatag gtgatatttt ctattctaaa ataacaagtt
     7621 gttgcatcgt agctaaggat ttatctaaaa atactatgaa attggacgtc atattttttg
     7681 aggataaaaa taaaagatcc gcagttttag gtttacgaag agacaaaagc ggagtattta
     7741 aaccagttac cctacatttt acaagcgcta agaaatatgc taaagttcgt aaaacagatg
     7801 tgaaagaaat gaaatggcta taaagattgt ccgcaggctt aagcttgcgt tttttgttgt
     7861 ttaacgaaat taaaaaaaca agctctctca tcggaaatgt ttttatagga atgaaggaag
     7921 atgatgctat gtttaaaaag agaatcgaaa agggcaaaag tagtgtcttt atctttttgg
     7981 agtagaagct tatgatatag aaatttgtaa taaaaatgta atataaaaca tttgacaaat
     8041 atttacgggg ggagtatact taatataagt atagtctaaa aatgactatc agaaaagagg
     8101 taaatttaga tgaataagaa aaaaatgatt ttaacaagtc tagccagcgt cgctatctta
     8161 ggggctggtt ttgttacgtc tcagcctact ttggtgagag cagaagaagc tcccgtagct
     8221 aaccagtcta aagctgagaa agactatgat gcagcagtga aaaaatctga agctgctaag
     8281 aaagattacg aaacggctaa aaagaaagca gaagacgctc agaagaaata tgatgaggat
     8341 cagaagaaaa ctgaggagaa agcagagctt gttcgaaaag cggatgaaaa acgtcaaaaa
     8401 gcgaatttgg cagttcaaga agcatatgta aaattccaag aagcacaaag agaatttaac
     8461 gaaagtccaa gcagaaaaaa atctgatgct aaaaagaaat tagatgatgc atcagctcat
     8521 attgaagagg taaaattaaa acaaaaagag gcggatgcga attttaataa ggaacaagca
     8581 aaagtaattc ccgaagcaag tgacttagct gttactaaac aaaaagcgga agaagctaaa
     8641 aaagaagcag aagtagctaa ggaaaaatat gataaggcag ttcaagaggt agaagtagag
     8701 aaaaataaaa tacttgaaca agatgctgaa aacgaaaaga aaattgacgt acttcaaaac
     8761 aaagtcgctg atttagaaaa aggaattgct ccttatcaaa acaaagtcgc tgaattaaat
     8821 aaagaaattg ctagacttca aagcgattta aaagatgctg aagaaaataa tgtagaagac
     8881 tatattaaag aaggtttaga gcaagctatc gctgataaaa aagctgaatt agctacaact
     8941 caacaaaaca tagataaaac tcaaaaagat ttagaggatg ctgaattaga acttgaaaaa
     9001 gtattagcta cattagaccc tgaaggtaaa actcaagatg aattagataa agaagctgca
     9061 gaagatgcta atattgaagc tcttcaaaac aaagttgctg atctagaaaa caaggttgct
     9121 gaattagata aagaagttac tagacttcaa agcgatttaa aagatgctga agaaaacaat
     9181 gtagaagact acgttaaaga aggcttagat aaagctctta ctgataaaaa agttgaatta
     9241 aataatactc aaaaagcatt agatactgct caaaaagcat tagatactgc tcttaatgaa
     9301 ttaggtcctg acggtgatga agaagaaact ccagctccag caccaaaacc agagcaacca
     9361 gctgaacaac caaaaccagc gccggctcct caaccagctc cagcaccaaa accagaaaaa
     9421 acagatgatc aacaagctga agaagactat gctcgtagat cagaagaaga atataaccgc
     9481 ttgccccaac agcaaccgcc aaaagcagaa aaaccagctc cagcaccaaa accagagcaa
     9541 ccagttcctg caccaaaaac aggttggaaa caagaaaacg gtatgtggta cttctacaat
     9601 actgatggtt caatggctac aggttggctc caaaacaacg gttcatggta ttacctcaac
     9661 agcaatggcg ctatggctac aggttggctc caatacaatg gttcatggta ttacctaaac
     9721 gctaacggcg ctatggctac aggttgggct aaagtcaacg gttcatggta ctacctcaac
     9781 gctaatggtg ctatggctac aggttggctc caatacaacg attcatggta ttacctaaac
     9841 gctaacggcg ctatggctac aggatggctc caatacaacg gttcatggta ctacctcaac
     9901 gctaacggtg ctatggctac aggttgggct aaagtcaacg gttcatggta ctacctcaac
     9961 gctaacggtt caatggcaac aggttgggtg aaagatggag atacctggta ctatcttgaa
    10021 gcatcaggtg ctatgaaagc aagccaatgg ttcaaagtat cagataaatg gtactatgtc
    10081 aatggtttag gtgcccttgc agtcaacaca actgtagatg gctataaagt caatgccaat
    10141 ggtgaatggg tttaagccga ttaaattaaa tcatgttaag aacatttgac attttaattt
    10201 tgaaacaaag ataaggttcg attgaataga tttatgttcg tattctttag gtacctatct
    10261 tatgatttca ggaagtgtca ttaaaaaaac gactcctttt ctctaacttg aaaaatagat
    10321 tagagaaaat gggttgtttt atttattata gttatttgaa tgaagataag aagagggtat
    10381 actcacgtca ttcacatgat ctgtatattg actataagtt ttaaaaaaca atttttaagc
    10441 tcttccttgt cttctctaac caagcgtgtt ataatgaata ctgctcaagc gaccttcaat
    10501 cgtgaagcac acacgacctt caatcgtgaa taaacgaata gatggagact taccatgagt
    10561 gataactcta aaacacgtgt tgtcgtgggg atgagtggtg gtgttgattc gtcggtgacg
    10621 gctcttttgc tcaaggagca gggctacgat gtgatcggta tcttcatgaa gaactgggat
    10681 gacacagatg aaaacggcgt ttgtacggcg accgaagatt acaaggatgt ggttgcggtg
    10741 gcagaccaga ttggcattcc ctactactct gtcaattttg aaaaagagta ctgggaccgc
    10801 gtttttgagt atttcctagc ggaataccgt gcagggcgca cgccaaatcc ggacgttatg
    10861 tgcaacaagg aaatcaagtt caaggccttt ttggactatg ccatgacctt gggggcagac
    10921 tatgtagcga ctgggcatta tgctcgagtg gcgcgtgatg aggacggcac tgttcacatg
    10981 cttcgtggcg tggacaatgg caaggaccag acctatttcc tcagccaact ttcgcaagaa
    11041 caactccaaa aaaccatgtt ccctttggga catttggaaa agcctgaagt acgcagacta
    11101 gcagaagaag caggcctggc gactgctaag aagaaagatt cgacagggat ttgttttatc
    11161 ggagaaaaga actttaaaaa cttcctcagc aactacctgc cagctcagcc tggtcgcatg
    11221 atgactgtgg atggtcgcga tatgggcgag catgcaggtc ttatgtacta tacaatcggt
    11281 cagcgtggcg gactcggtat cggtgggcaa cacggcggtg acaatgcccc ttggttcgtt
    11341 gtcggaaaag atctaagcaa gaatattctc tatgtaggac aaggattcta ccatgattcg
    11401 ctcatgtcaa ctaacctaga agccagtcaa gtccacttta ctcgtgaaat gccagaagag
    11461 tttacgctag aatgtacggc taaattccgt taccgtcagc ctgactctaa ggtgaccgtc
    11521 catgtcaaag gagacaaggc agaagttatc tttgcggaac cacaacgcgc gattacacca
    11581 ggacaggcag ttgtctttta cgatggcgaa gagtgtctag gtggcggttt gattgacaat
    11641 gcttaccgcg atggacaagt ttgtcagtac atttagattg acaaattttc tcaatttgct
    11701 acaataataa cagcaataga aatgatggtc aaagctcatg gatgttgcag gcttttttgt
    11761 cctgcacttc tttgtagttt tgactgtttt tgtgtcgttt aagggaaagg acaagaatga
    11821 ctcaacaaga ctttcggaca aaagtagaca atacggtttt tggtgttcgg gcgacagcct
    11881 tgattgtcca aaatcacaag cttctagtca ccaaagacaa gggcaagtat tacactatcg
    11941 gcggtgcgat tcaagttaac gaaaaaacgg aagacgcggt agtccgtgaa gtgaaggaag
    12001 aactaggtgt taaagctcaa gctgggcagc tagcttttgt ggttgaaaat cgttttgaag
    12061 tggacggcgt ttcctatcac aatatcgagt ttcattatct ggtggatttg cttgaagatg
    12121 ccccgttgac catgcaggaa gatgagaaaa ggcagccctg tgagtggatt gacttggata
    12181 agcttgagga tatccagcta gttccagcct ttttaaaaat agccctacca gattgggaag
    12241 gccaactaag acacattcat cttgaggaat aggagagaaa catgacttat cattttactg
    12301 aagaatacga tattattgta attggtgcgg gacacgctgg ggttgaggct tccttggccg
    12361 ctagccgtat gggctgtaag gtcctgcttg cgaccatcaa tattgaaatg ctggctttca
    12421 tgccttgtaa tccctctatc ggtggttctg ccaaggggat tgtcgtgcgt gaagtcgatg
    12481 ccctcggtgg cgagatggcc aaaaccattg acaagactta catccagatg aagatgctca
    12541 acacagggaa ggggccagct gtccgtgccc ttcgtgcgca ggctgacaag gaactttact
    12601 ctaaggagat gcgcaagacg gttgaaaacc aagaaaatct gacccttcgt caaaccatga
    12661 ttgatgagat tttggtggaa gatggcaagg ttgtcggtgt gcgtacagcc acccatcaag
    12721 aatatgctgc taaggctgtt attgtgacga cagggactgc tctccgtggg gaaattatca
    12781 tcggagacct caagtactca tcaggtccta accacagctt ggcttctatt aacctagctg
    12841 acaatctcaa ggaactgggc ctcgaaatcg gtcgtttcaa gacaggaacc cctccacgtg
    12901 tcaaggcttc ttctatcaat tacgatgtga cagaaatcca accaggagat gaagcaccta
    12961 atcacttctc atacacttca cgtgatgagg attatgtcaa ggaccaagtg ccatgctggt
    13021 tgacctacac caatggtacc agtcatgaga ttatccaaaa caacctccac cgtgcgccta
    13081 tgtttacagg tgtggtcaag ggagtggggc ctcgttactg tccatcgatt gaggacaaga
    13141 ttgtgcgctt tgcggacaag gaacgtcacc aactcttcct tgagccagaa gggcgcaata
    13201 ctgaggaagt ctatgttcaa ggactttcaa ccagtctgcc tgaggatgtc cagcgtgact
    13261 tggttcattc catcaaaggt ttggaaaatg cagagatgat gcggacaggt tatgctattg
    13321 agtatgatat ggtcttgcct catcagttgc gtgcgactct ggaaaccaag aaaatctcag
    13381 gtctcttcac tgctggtcag acaaatggaa catcaggtta tgaagaagct gctggccaag
    13441 ggattatcgc gggtatcaat gcggctctga aaatccaagg caagcctgag ttgattttga
    13501 agcgcagtga cggttatatc ggggtgatga tcgacgactt ggtgaccaag ggaaccattg
    13561 aaccctaccg tctcttgaca agtcgtgctg aataccgtct cattcttcgt catgacaatg
    13621 ctgatatgcg cttgactgag atgggacgcg agattggcct tgtggatgat gaacgctggg
    13681 ctcgttttga aatcaagaaa aatcaatttg ataatgagat gaaacgccta gacagtatca
    13741 aactcaagcc agtcaaggaa accaatgcta aggttgagga aatgggcttc aagccgttga
    13801 cagatgcggt gacagccaaa gaattccttc gccgtccaga agtttcttac caagatgtgg
    13861 tggccttcat cggaccagct gcagaagact tggatgacaa gattatcgaa ttgattgaaa
    13921 cagaaatcaa gtacgaaggc tatatttcca aagccatgga tcaggttgcc aagatgaaac
    13981 gtatggaaga aaaacgcatt ccagccaata tcgactggga tgacatcgat tctattgcga
    14041 cggaagctcg tcagaagttc aaactcatca atccagaaac catcggccaa gccagccgta
    14101 tttcgggagt aaacccagca gatatttcta ttttgatggt gtatctggaa ggtaaaaatc
    14161 gtagtatttc taaaactctt caaaaatcaa aatgatacgt cgtcggcttc ttacgaatga
    14221 gttcaaagct tggctttgat tcatctccaa cctcccatag ttccccgaac tatgggagct
    14281 aactcaagtt atgctttcgc ttagccttct agtctgattt tcattgagtt ttaatctcaa
    14341 aagaaagcat agagaaaaac agctccgaag acggggctgt tttgttgatt tgtactcaat
    14401 gaaaatcaaa gagcaaacta ggaagctagc cgtaggttgc tcaaagcact gctttgaggt
    14461 tgtggataga actgacgaag tcagctcaaa acacagtttt gaggttgtgg atagaactga
    14521 cgaagtcagt aacatatata cggcaaggcg aagctgacgt ggtttgaaga gtattattct
    14581 tcatctggtg tgaggatcat cgggatgatg atcggttcac gttcggtatt ttcatagagg
    14641 aaggggcgaa tagcgttgac aatggcacca ttgacagatt gcacgctagc atccttattt
    14701 ttcagtgcga tacgaatggc attgaagagg atacgctggc tttggcgaat caagtcgcca
    14761 gactctctca tgtagacaaa gcctcggctg aggatgtctg gaccagatag aatcatctgc
    14821 gatttgaagt caacagttgc gactgccaga acgacaccgt cttcagatag atcgcgacga
    14881 tctttgagga cagctgcgcc aatttcaccg atacgatttc catcgacata gatatcttgg
    14941 gcgttgaaat gacctgcgat acgagctgag tcagcagtaa gggcaagcac atcgccattg
    15001 ctcatgataa agatattgtc cttctcaaca ccagtatcca ctgctagtcc agcgtggact
    15061 ttttgcatgc ggtattcacc gtggacaggc atgaagtatt ttggcttaat caagcagagc
    15121 atgagttttt gctcttgctg accaccgtgt ccagatgtat ggatattgtt tactttaccg
    15181 tggataactt cgacaccagc ttcagaaatg atgttaatca gcttgttgac gctagtagtg
    15241 tttccaggga tgggactaga agagaagata acggtatcac ctggttgtaa ttgtacttga
    15301 cggtgggttc cgttggcgat acgagagagg gctgccatag gctcaccctg actacctgta
    15361 cagaggataa gaacttctcc tgcaggataa tctttgattt catttggctc gataaaggtt
    15421 cccttaggag ctttgatgta gccaagatcg attccgttga caatggcctt ttccatagaa
    15481 cgaccaaaga ccgcaatctt gcgtccagtc ttaacagcag cttctgttgc ctgctggaga
    15541 cggaagatat ttgaggcaaa ggatgcaaag atgatacgtc cttcaatacc ttggataatc
    15601 ttcataatgg actgaccaac gactttttca gagttggtaa aggttggtac ttccgcattt
    15661 gtcgagtcag acaggagaca gagcacgcct tcttcaccaa gcgcagccat acgatgcaag
    15721 tccgcaggtt ctccaactgg agtaaagtcg aacttaaagt cacccgtaca gacgattttc
    15781 ccttgaggag tatgaatgac aatccccaaa ggctctggaa tagagtgagt cgttctaaag
    15841 aaagttgcct tgagattttt aaaggtcaac tcggtgttgt ggttgatttc gtaaagtttg
    15901 gcgttgcgca agaggccgtg ttcttcgagt ttcccacgga tcaaagccaa ggcaagcggt
    15961 ccagcgtaga tagggacatt tgcttgcttg agtaggaacg gaatcccacc aatgtggtcc
    16021 tcgtgtccgt gtgtgattaa aacagccttg acgcggtcga tattgtccac gatgtaagag
    16081 tagtcaggaa tgacatagtc gataccaagc aagtcatctt ctgggaattt aatcccagca
    16141 tcgacgatga taatctcgtc ttggtattca attccgtaag tgtttttccc gatttctcct
    16201 agaccaccga tggcaaaaac gccgacttct tcaggtttaa gagtgtaggc catattagaa
    16261 ctccgtaatt tcgaaggcgc cagtttcttt ttcgtaatcg agcaatttgt cagacaagag
    16321 ttcgatatat tcgatattgt actctgggcg attttcttcg acaagttggc gagcagtgat
    16381 acggccctca agttctgagc tggcatcgat gtctaggtaa agcgcgcgtg ttgtttcacg
    16441 gcgtgggcta cgttcttttg tttcttgata aaaaactttg taaatcatat agttcctttc
    16501 tatttacagg tcagcttatt ccaaactttt agcagagatt tgcttgattt cattccattt
    16561 tgtgtctaga ttgaccttga ttcgttacaa ttatttcaat tataccacaa aatgaaaaat
    16621 tagtaaaaga cggaaacgag aaagttaggg gacgatttct ttggtaagcg tttgcaaaaa
    16681 taaagaaaat gtattatgtt gataacggaa gtgaaattta ttgcttgtca taattccata
    16741 tagattttaa gtcaaggagg ttgccctatg tataacttat ttgcttttgc tatcggagtt
    16801 cgtttgctag cttttatcgt ttttgttgct tgcgtttatg gaggaaatct tctatttgcc
    16861 aaactggaac gacgacaaac caagttcttg tggaaaatga cctttgtgac tctctactcg
    16921 ctttttctcc tcctcgtgac ctatgtcgtt tggtttgtat tttactttga attaaatgct
    16981 tagatgccct gcttatgctg gggttttttg tttggggatg tagagatagt agatttacga
    17041 cacgaaaatt ccaccccatc tatcaagatg aggtggtctt tgcatttttc taacatgaca
    17101 tgacaatgga attttcaaag cttattattt ttatgggagt cattgttctg atcaaaaata
    17161 gagttgaagc ataaaaataa aacaatacaa acagataaat cgaatggtaa agggatggta
    17221 gtaatgttca aatcattgag aatgctgagc aaactgtaag agatgaagac aataaaattt
    17281 atccttctaa aaatgtgttt cttatctagc atgacttacc aaacttttta cgaaggtctt
    17341 ttccaatggt ataacctgcc ataaataaag cgataccaac accgacacca aagccaccct
    17401 tgatttcttg caattcctta ttagaaattt cacgataatt gttcaaatct ttcatcatat
    17461 taatctcctc ctaaaaaata accagctgcc aaaccagaac ctataagtgc tgttccacct
    17521 gcaatagcct tccaagatac tgctactcca aagataacca agggagcgag accgccatca
    17581 atatctgata attcattttc agataaggat aaaaattgat tgtcaagaat gtcaaaattt
    17641 gtcattgaac tttcctcctt ttttcttgtt cacttttata ttcggaaaag aaaaggaaaa
    17701 gtgagcattt ttttaaatta tttttagtta agtgagggca ctcttgtctg ggggcaaaga
    17761 aaattcccat gtgccagctt ttgtagtata atagtaagca gacaaaaaga taggaatgtg
    17821 ttatgaaagt attagctttt gatacgtcca gcaaggctct ttctctggct attttagagg
    17881 ataagcaggt tcttgccgag acgacgatta atattaagaa aaatcacagt attactctta
    17941 tgcctgccat cgattttttg atggcaagtt tggattggac acccaaggat ttggaccgaa
    18001 tcgtggtagc tgaagggccg ggtagctata caggcttgcg aattgcggta gcaactgcta
    18061 agaccttagc ttacaccctg aacatcgagt tggttggtat gtcgagtctc ttggctctgg
    18121 tgccccacca acaagaaggt ttgtttgtcc ccttgatgga tgcgcgtcgc aataatgttt
    18181 atgcaggatt ttatgaaaat gccaaacctg tcatggcaga agcgcaccta tcttttgaag
    18241 aggtgctaga aaaagtcaag ggtactagtc aggtaacctt tgtcggagaa gttggcccct
    18301 ttgttgagca gattcaaaaa cacttgccaa ggactgatta caaagaaaca ttgcccaatg
    18361 cagccaatct tgctctttta gcttgggaca aggaagcaga ctccttgcat gattttgtgc
    18421 cgaactacct caagcgtgtc gaagctgagg aaaactggct caagaatcac accgagtctg
    18481 gtgagtccta cattaaacgc ctatgataga aatcaagcga attcaacaac atcctgacct
    18541 agctcaagcc atctacgctg ttatggcagc tgtttaccta gtcagtcctt ggactctgga
    18601 gcaaatccaa gcagatctgt cccaagacca gacttggtat gcattggctt atgatggggc
    18661 agaagtgatt ggatttctag ctgtgcagga gaatcttttt gaagcagaag tcctgcaaat
    18721 cgctgtcaaa ggagcttatc agggtcaggg aattgcgtca gccttgtttg ctcaattgcc
    18781 gacagacaag gaaattttcc tcgaagtcag acagtcaaat caacgagcgc aagcatttta
    18841 caagaaagaa aagatggcag ttatcgctga gcgaaaggcc tactaccatg acccagtcga
    18901 ggacgccatt atcatgaaga gagaaataga tgaaggatag atatatttta gcatttgaga
    18961 catcctgtga tgagaccagt gtcgccgtct tgaaaaacga cgatgagctc ttgtccaatg
    19021 tcattgctag tcaaattgag agtcacaaac gttttggtgg cgtagtgccc gaagtagcca
    19081 gtcgtcacca tgtcgaggtc attacagcct gtatcgagga ggcattggca gaagcaggga
    19141 ttaccgaaga ggacgtgaca gctgttgcgg ttacctacgg accaggcttg gtcggagcct
    19201 tgctagttgg tttgtcagct gccaaggcct ttgcttgggc tcacgaactt ccactgattc
    19261 ctgttaatca catggctggg cacctcatgg cagctcagag tgtggagcct ttggagtttc
    19321 ccttgctagc ccttttagtc agtggtgggc acacagagtt ggtctatgtt tctgaggctg
    19381 gcgattacaa gattgttggg gagacacgag acgatgcagt tggggaggct tatgacaagg
    19441 tcggtcgtgt catgggcttg acctatcctg caggtcgtga gattgacgag ctggctcatc
    19501 aggggcagga tatttatgat ttcccccgtg ccatgattaa ggaagataat ctggagttct
    19561 ccttctcagg tttgaaatct gcctttatca atcttcatca caatgccgag caaaagggag
    19621 aaagcctgtc tacagaagat ttgtgtgctt ccttccaagc agcagttatg gacattctca
    19681 tggcaaaaac caagaaggct ttggagaaat atcctgttaa aaccctagtt gtggcaggtg
    19741 gtgtggcagc caataaaggt ctcagagaac gcctagcagc cgaaatcaca gatgtcaagg
    19801 ttatcatccc ccctctgcga ctctgcggag acaatgcagg tatgattgcc tatgccagcg
    19861 tcagcgagtg gaacaaagaa aactttgcaa acttggacct caatgccaaa ccaagtcttg
    19921 cctttgatac catggaataa agagtggctc tttgtcaagt gtagtgggta ggcgaaaagc
    19981 tacaatctgg agagtacgaa attcgtactc tcttttttga tgttcaaagt catcaaaatc
    20041 cgttttttaa aattgttaaa gttccggaaa ccaaaggctt tgcgcttgat aaccttaatc
    20101 aaattattgg tcgcttcaag tttagcgttg gaataaggca gcttaagtgc gttgataatc
    20161 ttatctctat cccttaaaaa agtccaaaag accgtttgaa aaagcggatg aaccgttggc
    20221 agttcttgct caatcaatcc aaagaaatgg tcggcattct tctcttgaaa atgaaagagc
    20281 aggagttgat agagttcgta gtaaacctgt aatccctcag agtaagaaag cagcttatct
    20341 cgtacttccc gatgggttaa gtgcattcga aacgtgggct gatagaatcg tttctgacag
    20401 agcttgaagc tatcctggtg tagtaaatca gtccagtgga ctgatttagc ccgagcctag
    20461 aaacgaaaaa gcgagggttc caaaagcgct tgagcgcccg atactccaag gattttcggt
    20521 caaattggtt catgatttgg attcgtactc ggttcatagc tcggctcaga tgttggacaa
    20581 tgtggaagcg gtcaagaaca atcttcgcct ttggaaagag ttgcttagcg agccgataat
    20641 aagggctgta catgtccatg gtgatgacct cgaccagctc ccgaacctct ctctgatagc
    20701 gttggaaatg gtttcgaatc accgcatgag ttcgcccgtc taaaattgcg aggatggatt
    20761 tggactcaaa atcttgggca atgaagctca ttttgctctt tttgaagcta tactcatccc
    20821 aactcataac ttttggcaac ttggtccaat cggtttcaaa cttaaattcc ctcagttttc
    20881 ggatgacggt ggaagttgag accgccaatc tgtgggcaat ctcagtcata gactgctttt
    20941 caaggaggag ctgagcgatt ttttgtctca ccatgttgga aatctggcaa tttttcttga
    21001 caatggatgt ttgagaaacg accaccttaa ggcaattctt gcactgaaag cggcgctttt
    21061 tgagatgcag taccgtgggt aaaccctgac agtcgagaag cggaatttta gaggctttct
    21121 ggaagtcgta tttgatcatc ttcccttgac aatgaggaca aggaggagcg ggggaatcca
    21181 actttgcctg aacgactaga tgggtttggt atttcagaac agacaagatt ttgatatttt
    21241 tgtcttccaa tccgagcaaa tctgtggtat tcttaagttg ttccatatga accctttcta
    21301 atgagttggt tagacgcttt tcattatagg tcatatgggg ctttttttct acaagaaacg
    21361 accctataat tcctggggtg ggattaccca ctacagaaat tatagagcca aagcattcca
    21421 aagtcttgtc tgactctgga atgttttctg ttcacagttg ttttgtaact gcctaataag
    21481 taaccgcgct ataacgaggt atctaccgag attgtcaatt ctccagtata ctgttataaa
    21541 aaacaaaact ggaggatttt tttatgtccc cccatcgtcc catcgactgt tccgtagctg
    21601 cccaataacg gagtatagtc ggctgaaaca agaataggac aaaagtgcct caagaaaagc
    21661 atcgtaattt gacaatatta ttctgagatg tttttgatgt aagcccatat tttctcaatg
    21721 ggattatact cgggtgaata ggtaggaagt ggtaacagtc tatgccctgc tccttacaca
    21781 tgttcattct gtgaaacctt gcattgtcca taatgataag ggatggtgta tctaaagtag
    21841 gtagtaagaa tttttggaag caagcttcga aggagccata gtatattgaa acaaaaaagg
    21901 gcattagtac ttctataaca gtatcgcaat ctctggtgtg cgttgtctcg atcttaattc
    21961 aaataattat atcttcttcc agagagacta actgcctgct caaggctcga tcatattcga
    22021 gataaaaata ggtcttaacc cctgtctcat aaatataaat aggagtcagg tagcttaagt
    22081 gattcaattc tttaaggaac cgatttactt tttctgggtc ttgttcgcag taggtacggc
    22141 tcttttttag actatatccc atagccttta gagcgtaatg aatagttgtt ggaggacagt
    22201 tgaattcagc agctatttca gtcaaataag catcgggatg agtttcaata tagatcttta
    22261 atttatctct atcaactttt tcaattaata gtggaagttt tcttgaagtt agtgtattga
    22321 aacgttttta tacagtgcta aaatttgaag tgtagtgcag atgtatttgt aatagtatta
    22381 tagcaattct ttggagaagg tcgagacaag agaaaatagg tatctttgag atataagcat
    22441 gatttactat aaaattattg agttattcat aaaaatttct ctatcaaata tcataatgcg
    22501 tcgtttaagg agatggttac aatgcaatat agttgtggaa aaataaatat taatatccct
    22561 gatggatatg gagacattaa ggatatagtg ttttcggctc atattatagt taggtataat
    22621 aatggacatt gtggaggtat agatccgcat ataattggac tttgtaaaaa gcagatcaga
    22681 aggatgtctc tatatcctat tttgataatc gtatctagag attcaaaggt tattgatgat
    22741 tataaaaatt tagatattgc ctatgttgat tgtactcaat gttcaaataa ttttgaaact
    22801 gccttacacg ttaaaaatat tttaaaatta
//
LOCUS       DAVKQB010000034        22759 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKQB010000034 DAVKQB010000000
VERSION     DAVKQB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22759
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.034"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            491..1009
                     /locus_tag="VW528_001375"
     CDS             491..1009
                     /locus_tag="VW528_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9912186.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            1139..1645
                     /locus_tag="VW528_001376"
     CDS             1139..1645
                     /locus_tag="VW528_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9912187.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            1645..1815
                     /locus_tag="VW528_001377"
     CDS             1645..1815
                     /locus_tag="VW528_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912188.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            1847..3079
                     /gene="clpX"
                     /locus_tag="VW528_001378"
     CDS             1847..3079
                     /gene="clpX"
                     /locus_tag="VW528_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9912189.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            3088..3675
                     /locus_tag="VW528_001379"
     CDS             3088..3675
                     /locus_tag="VW528_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9912190.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            3686..4066
                     /locus_tag="VW528_001380"
     CDS             3686..4066
                     /locus_tag="VW528_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9912191.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            4118..5008
                     /gene="rapZ"
                     /locus_tag="VW528_001381"
     CDS             4118..5008
                     /gene="rapZ"
                     /locus_tag="VW528_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9912192.1"
                     /translation="MTKKQFHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            5005..5982
                     /locus_tag="VW528_001382"
     CDS             5005..5982
                     /locus_tag="VW528_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9912193.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            5979..6890
                     /gene="whiA"
                     /locus_tag="VW528_001383"
     CDS             5979..6890
                     /gene="whiA"
                     /locus_tag="VW528_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9912194.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(6978..8105)
                     /locus_tag="VW528_001384"
     CDS             complement(6978..8105)
                     /locus_tag="VW528_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9912195.1"
                     /translation="MWMEELPNGKFKYFERYKDPYTEKLKKVSVTMEKKTPQARNQAA
                     ILLQEKINKKLSTKQVESITFEEIYNLFYKSWAQTVKESTKHNCKSVDKKMKEVIPSD
                     TILANLDRRFLQEAIEKIIESNGYITAKKVRHRLRGIFNYAVQYSYIENNEVDYTTIP
                     QKPKTLEELEKKRNNFLTMQEIKALVDVLNRREYHQKYADMVLVLTLTGMRYGELTAL
                     QLKNIDFENNKIEITGNFDSVNKIKTLPKTTNSIRTIKVSESVIEAIQRQIVRLSERF
                     QPLSSDDYIFCFEKWNQPTTIACFIQILKKYGKQAKIEKNLSSHIFRHSHISFLAESD
                     LPIKSIMDRVGHSNAKMTLEIYSHTTEDMEDKLVNKLDTIF"
     gene            complement(8384..9334)
                     /locus_tag="VW528_001385"
     CDS             complement(8384..9334)
                     /locus_tag="VW528_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEU9912196.1"
                     /translation="MKPFKTIEEQIATLKIRGLSITDESKAAKYLLSNNYYNIINGYS
                     KFFQHPGTDTYIDGVTFDEVSSLYTFDKDVKRAILQAILEAEHHIKSITAHRFAEAYP
                     SQKYAYLNTNSYADNKILDVGFIVSKLSKIINTNKRYRGNSIHHYAHTHSDVPIWVLT
                     DYLEFGDLRTIIENLPNSLQNEIARDLVSFISTNIPDFNDVFPPETLISFLKNINEVR
                     NKCAHNNRLLNFRCRSNSTFWETIHNKEILMGDDSRKTVYSTIISLQCFISKAAFNIL
                     WNTLRKKVIKLEKKLPSIDINVINQSLGFPNDWHRNEPKV"
     gene            complement(9483..10274)
                     /locus_tag="VW528_001386"
     CDS             complement(9483..10274)
                     /locus_tag="VW528_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9912197.1"
                     /translation="MDEKKRMQIIAENITHFRKQRGITQKELAKEVGITASTMTDYMK
                     LRSAPSFGVIQKLADYFGVKKSDIDTTFKEESTNSLPDAPDSLTQQIMDKVVQLTPPN
                     QKIVLRTSEELLESQNEEETKINEVSEVISLYQVEVVSETAAASGFNYGFGYDDTDRE
                     TIEVDERPPRHDIATKVSGDSMQPDYQDGDILYLVDKGLTTYNGDLAIIAYGDRSYFK
                     KIYTENGRLRLVSLNDKYEDIILDFPPAEDTHIKIYAVVGVYRGE"
     gene            complement(10518..11207)
                     /locus_tag="VW528_001387"
     CDS             complement(10518..11207)
                     /locus_tag="VW528_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEU9912198.1"
                     /translation="MIFRVKVNSSVSRPVTVEDICPNCKKPTNPDLVNSSYFSLGENK
                     TSLVLTCRCLGCKHFWTEEFIATRFLINDYTEKYEIEHIKVIPNLPSDIPISDDVEIV
                     SPIGKQIYVQALKAEHEQLDHIAGIGYRKALEFFVKDFSIVTNPDDEDKIIKMSLKQV
                     IEKYIKDEDLKTFALASAYIGNDEGHYYRNNPDKGFTDLKNYLHGVIHYMEMKLNFLD
                     AQELVNRSKKS"
     gene            11414..11578
                     /locus_tag="VW528_001388"
     CDS             11414..11578
                     /locus_tag="VW528_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912199.1"
                     /translation="MKENIEHLHERIKHFQSLIAYMSEHEKKYYLEKDWFDNPTLISI
                     EDAKKEVELL"
     gene            11597..11812
                     /locus_tag="VW528_001389"
     CDS             11597..11812
                     /locus_tag="VW528_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9912200.1"
                     /translation="MQWTLEAMRINKGLTQAELAEKFEVSSQTIARLEKDSSDIGYQL
                     LKKYMFFFNVKFDDIFLGKKYENFVNN"
     gene            11844..12044
                     /locus_tag="VW528_001390"
     CDS             11844..12044
                     /locus_tag="VW528_001390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912201.1"
                     /translation="MESVLESLKQEKDHLEKIIKVVTSGGKFLRLPYQKKSRSISENL
                     KLISQNLDKLSEQVQQTTNQHS"
     gene            complement(12443..12634)
                     /locus_tag="VW528_001391"
     CDS             complement(12443..12634)
                     /locus_tag="VW528_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912202.1"
                     /translation="MKSFKDFRESLTAEDMQAISAKANEATKQIDHTDGLQLGKVSGL
                     TSVITTIELLEKYHEWLHS"
     gene            12753..12896
                     /locus_tag="VW528_001392"
     CDS             12753..12896
                     /locus_tag="VW528_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912203.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     RTV"
     gene            12893..13063
                     /locus_tag="VW528_001393"
     CDS             12893..13063
                     /locus_tag="VW528_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912204.1"
                     /translation="MKEAVKEFLKFRSRFTKIEWFEINQAVEARLNQKADQLKLDDVD
                     LEIISSRLEKVI"
     gene            complement(13056..13295)
                     /locus_tag="VW528_001394"
     CDS             complement(13056..13295)
                     /locus_tag="VW528_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912205.1"
                     /translation="MTEIHACLCGNWVNLSADDDCVMGPNMASPYIWWEENAELYSPI
                     SKPEANSMYHQDYIYIHYRGADYRIHPMFIQIVSR"
     gene            13413..13685
                     /locus_tag="VW528_001395"
     CDS             13413..13685
                     /locus_tag="VW528_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912206.1"
                     /translation="MEAVEIVRIKDVIIEKVSANDEELKRIFGCSKRQAGERRREMQK
                     LPSQQKHLLDSGQLVTIKGFYEYLQYRGTKAWKKEMETSKKMRSAG"
     gene            13682..13897
                     /locus_tag="VW528_001396"
     CDS             13682..13897
                     /locus_tag="VW528_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912207.1"
                     /translation="MNLLSRIKNYFSEEVKETNLDWKEVALDLNQSLIETQEKLQEAN
                     QEIADLKKIVAIYKEKEKEKRWNIFTW"
     gene            13876..14064
                     /locus_tag="VW528_001397"
     CDS             13876..14064
                     /locus_tag="VW528_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912208.1"
                     /translation="MEYIYLVTIVGIGLWSLVNKLDDHAEMKQKERQLMANNVARMNL
                     RNSDKQFTYDVEPPEGLK"
     gene            14076..14417
                     /locus_tag="VW528_001398"
     CDS             14076..14417
                     /locus_tag="VW528_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912209.1"
                     /translation="MTQAERIREYYREHPAASYDEVAEVVGTTNSNVRANLAKDIKAG
                     RCVRLEDKSYDYSPYYNHTQALTELVDWKNDNRREWVDMLTRAAEKETDSNVMRLLIK
                     EANKLMKEVTK"
     gene            14418..14648
                     /locus_tag="VW528_001399"
     CDS             14418..14648
                     /locus_tag="VW528_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912210.1"
                     /translation="MVRNKIGDLTNTLFAQLETLDDRDLTADELKVELHRSKQMVAIS
                     GQILQAGQLALDAEKFKDKVGEVNAPIALLEG"
     gene            14662..15384
                     /locus_tag="VW528_001400"
     CDS             14662..15384
                     /locus_tag="VW528_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU9912211.1"
                     /translation="MHEICPGRLTPEVTRLLNEKFGTNYNKSQIGGVRKRLGLAVGKV
                     YQGRLLTKEQHDYLVLIQKNKISRNVANEMNQKFGLSLTEKQIKSYRRNNNLHSGLTG
                     RFEKGQTPHNKGKKYPNMPKNGGQFKKGNRPPNYVPVGTINYTTNGYPKEKIGEPNQW
                     VLKHRKVWEDHHGLIPKGYSIVFLDGDKTNYDISNLACLSKNEIARMNQNHLFTSNAD
                     LTKSGIGLTKLTNKIREVEKNG"
     gene            15377..15859
                     /locus_tag="VW528_001401"
     CDS             15377..15859
                     /locus_tag="VW528_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEU9912212.1"
                     /translation="MASLYELTGQFLTIYQLDIDDETKADTLEAIDWQEQFEQKAEGY
                     AHVIKNLEADVAMYKAEEESFKAKKQVAQKKLDYVKDNIMAAMNVTGQTEVKSGALII
                     KIAKNPESVKVNEDDLPKKYFTKKVTLAPDKKTLKELLKSGKKVKGAELVRTEKLVIK
                     "
     gene            15859..16218
                     /locus_tag="VW528_001402"
     CDS             15859..16218
                     /locus_tag="VW528_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912213.1"
                     /translation="MELMNKTRVTDSLAVVIGPESIEVLVTEGFLFDVAIRFVKVDET
                     NLDQGNEKPVFTPEYKLVTVAKYKEKPIFESEEDIRKFEKQAKEVKSLFAFAKVNKQN
                     WFNTALYPGVLTEKVGV"
     gene            16218..16703
                     /locus_tag="VW528_001403"
     CDS             16218..16703
                     /locus_tag="VW528_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000696057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912214.1"
                     /translation="MKILAIDPSSNKIETSTTGVVLLDNARLVDSWVVSYGMRGFADW
                     FHEIGTNLEFDVVIVEEFKARDNDKSKDNSVAETIAYIQLCYPGAILQFNAGYKSDIP
                     NDLLKILDLWKFEKSHHQDIRAAARLGLFWAMRNDIEEVVHDIGKVVSEYHNNAKKVA
                     S"
     gene            16666..17859
                     /locus_tag="VW528_001404"
     CDS             16666..17859
                     /locus_tag="VW528_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HEU9912215.1"
                     /translation="MSITITLRKWQAEAIKRSEHLSNGIFLEALGGRGKTICALAIAK
                     HKKAKKIIITNNRLAILNGWIDAVKFMNFDKGVEIIIQTDRYLQNQVKKGHKLDCDVL
                     IVDEWQNMSSDKQVALYRKIKRKYTIGLSATPIRKKGQNFYPLEKTVFGWATPNNKFD
                     WQKTHGKMVYDPFSYSKEKWEDFQNYESYISSLPNFFRWEEIEGIENAVENNGFEIKF
                     YQKRVASGNPEKLAEFRKLNLVTVDGKTAMAKQSFGRKTFERYLNQTGVAVDFPKLKP
                     VNADTPLMLQLDGLIERAPHDMLIVSKSKQIVNVISERHPEIGIWTGDIQEGLYKKFV
                     VATSQVLGVGVDGLQHKYQTIVVLDPVEEGSGEYDDYRQLLWRITGSRQQHDVNVIEF
                     YYKES"
     gene            17877..18194
                     /locus_tag="VW528_001405"
     CDS             17877..18194
                     /locus_tag="VW528_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912216.1"
                     /translation="MNKTTEMIVFRSRKTGEFLNSYKDRSSLAFAADFCSLEYCLKLP
                     RKKYEDNKKTYKALAAAFDCEIVAVEAEYKLTYPNGSEVEPIKRDRSSIEDMIKDIIG
                     GVL"
     gene            18194..19021
                     /locus_tag="VW528_001406"
     CDS             18194..19021
                     /locus_tag="VW528_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9912217.1"
                     /translation="MAFTLPANKPQVPKDTPRNFFIYGETMSGKSYLANEFPNPIVLN
                     TDGNAEANTVPSIQLINEKDDKGRITNSVIKQLGDILLALQTQKHSYETVVIDVIDDV
                     IEMIKIAVCDELTPVGKPRLKSLSEIPYGKGYDFFNQAITELVIDLKALPMNVIYISR
                     QVSEYDDNGNATKDKPSLKDKYVNLINGNSDLMIHTEKLGNNYNREVDRKRKTYYADQ
                     VDDKAILKILATIRGAVEPAKGKLAPKKEAAKTTKPAKTEKTKEAPKKEVDSDDELF"
     gene            19041..19547
                     /locus_tag="VW528_001407"
     CDS             19041..19547
                     /locus_tag="VW528_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912218.1"
                     /translation="MSLLDIAKSIKKEGFDPRKDSANGPAPIPAGTYPVVLKKATFNV
                     SDKGWESLGYQFEIRGGDYSGRSEFATFGTLTEWNGKNLDWAVERTMKFFIKALVLAG
                     DSMQGNEEDGKALEEALQRKAVGSYYNLVISVTKGKDGREFRNYDLEEEEAQPLTEAD
                     IDEDDLPF"
     gene            19720..20541
                     /locus_tag="VW528_001408"
     CDS             19720..20541
                     /locus_tag="VW528_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEU9912219.1"
                     /translation="MPSMKEYALQYQKLGFSVIPINPKNKMPLIEFADKPAMTPSEIE
                     NFWDGYPNANIALKTTNFFVIDIDKHGKSNGFESLKKWKHLNLIEPTLQAKTASGGKH
                     LFYFKREDEPITQMIGFLPGVDIKAHENNYVLVAPSATDKGQYEWDLEKSKEGGTMVT
                     PSKYLIQSIKKQYGETHGYKYDGKDGLRDLVRRSHTRDRTQTTDLFETIALGFGDEGG
                     RNDKLAKFVGGLLYRAVDDGVVVQLARLANANSPTPLPEKEMMRTIESMIKKDRR"
     gene            20544..21887
                     /locus_tag="VW528_001409"
     CDS             20544..21887
                     /locus_tag="VW528_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9912220.1"
                     /translation="MIGNVVSIDSQPKMITTAKGDIKANSPSNVLMSFKADDQLSIYL
                     KHNDFSQEHELLKDIKIGNTLFKKGELPSNFDSVVKVYFESVLGVAFSSQAMLDGMET
                     FFSERSYNPVIEYMEKAAEKWDGRKRIDRMLQVYLGAEDIPLVSKIAQMWLVGAVAKV
                     YDPYVKFDYVLDLVGGQGVGKTSLLQKLGGEWYTDAVTDFSNKDNYDIMLKSLIVNDD
                     EMVASNRMSFAETKAFISKTSLRYRKPYMKRTEEFAKNFILARTTNQTEYLKDKTGER
                     RFLPVMADSKRQKKHPMEIEPETIEQIWGEAVTIYRAGADLMFDENTEDELNIYREQF
                     MYRDEVELQVLEYLDMPVPENWQNWSIQQQHQYTSKYFDNSSDFDPGSKKLDKVSTRE
                     MMYNLFMRNSNDRKLSTKINMIMDNHPDWKKSVFRAGGKSTKGFVRVKDSEKTNR"
     gene            22180..22335
                     /locus_tag="VW528_001410"
     CDS             22180..22335
                     /locus_tag="VW528_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912221.1"
                     /translation="MSYTVTLFFDNMVDETHFFKKESDAAKCKAQLESKYRGNRMYKV
                     KQEKLEE"
     gene            22332..>22759
                     /locus_tag="VW528_001411"
     CDS             22332..>22759
                     /locus_tag="VW528_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU9912222.1"
                     /translation="MNKQELIKRIEDLPYTEGPIADTIEINRNWILKSIEQLAESEIG
                     HADEAPRYVKNILARLRELPLHDREFWLKAIMSEFEQDFSHAKWREGYEQGKIEGMVE
                     REKVIVPQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVP"
BASE COUNT         7368 a         3981 c         4878 g         6532 t
ORIGIN      
        1 atttgaaaga gggatgtgat agtagcaccc tctttttctt tgttttatga tagttcatcc
       61 tcgagtaaat tcaagatctt gctcggaaat gaagcttata tagtagattg aatatagaca
      121 aagaccttgt gattggtaaa acattttaga aattcattta cctttcctaa tcgacttggt
      181 ttcatcttat ttcaatctat tatagtattg gggaatttct tcaaaccaca tcagcttggt
      241 cagttctacc tgcgacctca aaacttgtgc tttggtcaag ctgggtttag tttcctagtt
      301 tgctgatgga tttccattga ctataagcat ccaaccctct tttttgtctt ctaaagaatt
      361 cttaaattat cagtctattg caacttttct catataagtt ctttgtcttg ctattggttt
      421 tccttagtag tatactaagg tagtaatcat taagaagtgg ttacaaaaaa taatgaatga
      481 ggtaaagaaa atggtagaat tgaaaaaaga agcagtaaaa gacgtaacat cattgacaaa
      541 agcagcgcca gtagcattgg caaaaacaaa ggaagtcttg aaccaagctg ttgctgattt
      601 gtatgtagct cacgttgctt tgcaccaagt gcactggtat atgcatggtc gtggtttcct
      661 tgtatggcat ccaaaaatgg atgagtacat ggaagctctt gacggtcaat tggatgaaat
      721 cagcgaacgc ttgattacac tcggtggaag cccattctct acattgacag agttccttca
      781 aaatagtgaa atcgaagaag aagctggtga ataccgtaat gttgaagaaa gcttggaacg
      841 tgttcttgtt atctaccgtt acttgtcaga acttttccaa aaaggtttgg atgtcactga
      901 tgaagaaggt gacgatgtaa caaacggtat ctttgcaggc gctaaaactg aaacagataa
      961 aacaatttgg atgcttgcag ccgaacttgg acaagcacct ggtttgtaag aaataaggaa
     1021 acatagataa atctgtagga aatttcttac agattttttc tattttgtaa gctattccaa
     1081 accagtcttt ttttgagttt tttgataaaa tagtactatc agtgaaaagg atggaagcat
     1141 gactaagaaa atcgtagcta tttgggccca ggatgaagag ggtgtgattg gtaaggaaaa
     1201 tcgtctgcct tggcatttgc cagcagaatt gcagcacttt aaagaaacaa ctctgaatca
     1261 tgctatcttg atggggcgtg tgacctttga tgggatgggg cgtcgcttgc ttccaaaacg
     1321 ggaaaccctg attttgacgc gtaatccgga agaaaagata gatggggttg ctacttttca
     1381 ggacgtccag tctgttcttg actggtatca ggatcaagaa aagaatctct acattatcgg
     1441 tgggaagcaa atttttcagg cttttgaacc ttaccttgat gaagtgattg tcactcacat
     1501 tcatgctcgg gtggaaggag atacctattt ccctgaagag cttgacttgt ctctttttga
     1561 gacagtttca agcaaatttt acgccaaaga tgagaagaat ccttatgatt ttaccatcca
     1621 ataccgcaag agaaaggaag tctaatggaa cggagtttat ttggtctatt cacggctttt
     1681 ctgtgtttca tctgctttct ggcaggagca caggcttttc gtaaaaagcg ttacgggctt
     1741 tctatcctcc tttggttaaa tgcctttacc aatctggtca atagtattca tgctttttac
     1801 atgaccttat tttagataga atgacagtat agaatggaag gaaatcatgt ctacaaatag
     1861 aaaaaatgat atgatggttt attgctcatt ttgtggcaaa aaccaagaag aagtacaaaa
     1921 aataattgct ggcaacaatg cttttatttg taatgaatgc gtggagttag ctcaggaaat
     1981 cattcgagaa gaattggttg aggaagtctt ggcagacttg tctgaggtgc caaaaccaat
     2041 tgaactcctc catatcttga accactatgt aattggtcaa gatcgtgcca agcgtgcctt
     2101 ggcagtggcg gtttataacc actacaaacg catcaatttc cacgatacac gcgaagagtc
     2161 agaagatgtg gatttgcaga agtcaaacat tttgatgatt ggcccaactg gttcagggaa
     2221 aactttcctt gcccagacct tggctaagag cttgaatgtg ccttttgcta ttgcggatgc
     2281 gacagctctg acggaggctg gttatgtggg tgaggacgtg gaaaatatcc tcctcaaact
     2341 cttgcaggtt gctgacttta acatcgaacg tgcagagcgt ggcattatct atgtggatga
     2401 aattgacaag attgccaaga agagtgagaa tgtgtctatc acacgtgatg tttctggtga
     2461 aggggtgcaa caagcccttc tcaagattat cgagggaact gttgctagcg tgccgcctca
     2521 aggtggacgc aaacatccac aacaagagat gattcaagtg gatacaaaaa atatcctctt
     2581 catcgtgggt ggtgcttttg atggtattga agaaattgtc aaacaacgtc tgggtgaaaa
     2641 agtcatcgga tttggtcaaa ataataaggc gattgacgaa aacagctcat atatgcaaga
     2701 aatcatcgct gaagacattc aaaaatttgg tattatccct gagttgattg gacgcttgcc
     2761 tgtttttgcg gctcttgagc aattgaccgt tgatgacttg gttcgcatct tgaaagagcc
     2821 aagaaatgcc ttggtgaaac aataccaaac tttgctttct tatgatgatg ttgagttgga
     2881 atttgacgac gaagcccttc aagagattgc taataaagca atcgaacgga agacaggggc
     2941 gcgtggactt cgctccatta tcgaagaaac catgctagat gttatgtttg aggtgccgag
     3001 tcaggaaaat gtgaaattgg ttcgcatcac taaagaaact gtcgatggaa cggataaacc
     3061 gatcctagaa acagcctaga ggtgactatg gaacttaata cacacaatgc tgaaatcttg
     3121 ctcagtgcag ctaataagtc ccactatccg caggatgaac tgccagagat tgccctagca
     3181 gggcgttcaa atgttggtaa atccagcttt atcaacacta tgttgaaccg taagaatctc
     3241 gcccgtacat caggaaaacc tggtaaaacc cagctcctga acttttttaa cattgatgac
     3301 aagatgcgct ttgtggatgt gcctggttat ggctatgctc gtgtttctaa aaaggaacgt
     3361 gaaaagtggg ggtgcatgat tgaggagtac ttaacgactc gggaaaatct ccgtgcggtt
     3421 gtcagtctag ttgaccttcg tcatgacccg tcagcagatg atgtgcagat gtacgaattt
     3481 ctcaagtatt atgagattcc agtcatcatt gtggcgacca aggcggacaa gattcctcgt
     3541 ggtaaatgga acaagcatga atcagcaatc aaaaagaaat taaactttga cccgagtgac
     3601 gatttcatcc tcttttcatc tgtcagcaag gcagggatgg atgaggcttg ggatgcaatc
     3661 ttagaaaaat tgtgaggaaa agaaaatggc aaaaacaatt catacagata aggccccaaa
     3721 ggctatcgga ccctatgttc aaggaaaaat cgttggcaac cttttgtttg ctagcggtca
     3781 agttccccta tcccctgaaa ctggggaaat tgtaggagag aatatccaag aacagacaga
     3841 gcaagtcttg aaaaacatcg gtgctatttt ggcagaagca ggaacagact ttgaccatgt
     3901 tgtcaaaaca acttgcttct tgagcgatat gaacgacttt gttcctttta atgaggttta
     3961 ccaaacggcc ttcaaagagg aattcccagc tcgttcagct gtggaggtag ctcgtcttcc
     4021 tcgtgatgta aaagtcgaaa ttgaagtcat cgcagagatt ggataagcta gttgaagttt
     4081 ggtgttgcca aacttctttt gatataagga gaaaaagatg acaaagaaac aatttcactt
     4141 ggtgattgtg acagggatga gtggcgcagg gaaaactgta gccattcagt ccttcgagga
     4201 tctaggttat ttcaccattg ataatatgcc gccagctctc ttgcctaagt ttttgcagct
     4261 ggttgaaatt aaggaagaca atcctaagtt ggccttggta gtggatatgc gtagccgttc
     4321 tttcttttca gagattcaag ctgttttgga tgagttggaa aatcaagatg gtttggattt
     4381 caaaatcctc tttttggatg cggctgataa ggaattggtc gctcgttaca aggaaaccag
     4441 acggagtcac ccactagcag cagacggtcg tattttagat ggaatcaagt tggaacgtga
     4501 actcttggca cctttgaaaa atatgagcca aaatgtggtg gatacgactg aactcactcc
     4561 acgtgagctg cgcaaaaccc ttgcagagca gttttcagac caagaacaag cccagtcttt
     4621 ccgtatcgaa gtcatgtctt tcggatttaa gtatggaatc ccgattgatg cggacttggt
     4681 ctttgatgtc cgtttcttgc caaatcccta ttatctacca gaactgagaa accaaacggg
     4741 tgtggatgaa cctgtttatg attatgtcat gaaccatcct gagtcagaag acttttatca
     4801 acatttattg gccttgattg agccgattct gccaagttac caaaaggaag gtaagtccgt
     4861 tttgaccatt gccatgggat gtacgggtgg acaacaccgt agtgtggcat ttgctaaacg
     4921 cttggcgcag gacttatcca agaattggtc tgttaatgaa gggcatcgcg acaaagaccg
     4981 cagaaaggaa acggtaaacc gttcatgaga aaaccaaaga taacggtgat tggtggaggg
     5041 actggaattc ccgtcattct aaaaagtctg cgggaaaaag atgtggaaat cgcagctatc
     5101 gtgacggtgg cagatgatgg tggttcttca ggtgaactcc gaaaaaatat gcaacagttg
     5161 acaccgccag gtgatcttcg taatgtcctt gtggccatgt cggatatgcc taagttttat
     5221 gagaaggtct ttcagtatcg gttctctgag gatgccggag cctttgctgg ccatccattg
     5281 ggaaatctca tcattgctgg cttgtcagaa atgcagggtt caacctataa tgccatgcag
     5341 ttattgagca aatttttcca tacaacaggg aaaatttatc cttccagtga ccatcctttg
     5401 acccttcatg cagtctttca ggatgggaca gaagtggctg gagagagtca tattgtagac
     5461 catcgaggca taattgacaa tgtctatgtg accaatgccc taaacgatga tacgcctctg
     5521 gccagccgtc gagtagtgca gaccatcctt gaaagtgaca tgattgtcct agggccaggt
     5581 tccctcttta cctctatttt gcccaatatc gtgattaagg aaattgggcg ggctcttttg
     5641 gaaactaagg cagaaattgc ctatgtctgc aatatcatga cccaacgtgg ggagacggaa
     5701 cactttacag atagcgacca cgtggaagtc ttgcatcgtc accttggtcg cccttttatc
     5761 gacactgtct tggtgaatat tgaaaaagtg cctcaggaat acatgaattc caaccgtttt
     5821 gatgaatact tggtgcaagt ggaacacgat tttgtaggtc tttgtaagca agtttcgcgc
     5881 gtgatttcat ctaacttcct tcgtctggaa aatggcggtg ccttccacga tggagatttg
     5941 attgtggacg agttgatgcg cattatacag gtgaaaaaat gagtttcaca gtagcagtaa
     6001 aagaagaaat cctgggccaa caccatctga gctggcatga attatctgcc attatcaaaa
     6061 tgtctggtag catcggtctc tcgacttcgg gcttgacttt gtcagttgtg acagaaaatg
     6121 ccaaactggc ccgtcacctc tatgagtcct ttctccattt ctatgaaatc aaatctgaaa
     6181 ttcgtcacca ccaacggagc aatcttcgca agaatcgcgt ctataccgtt tttacagatg
     6241 aaaaggtgca ggatttgtta agtgatttgc acttggcaga ctctttcttt ggcctggaaa
     6301 caggtattga tgaggcgatt ttatcggatg aggaagcagg tcgtgcctat ctctgtggcg
     6361 ctttcttggc aaatgggagc attcgtgacc ctgagtcagg caagtaccag ttggaaatca
     6421 gttctgttta tctggaccac gcgcaaggaa ttgcctccct tctccaacag tttttactgg
     6481 atgccaaggt gcttgagcgc aagaaggggg ctgtgaccta tctccagcga gcagaagaca
     6541 ttatggactt cttgatagta ataggagcca tgcaggcacg tgatgatttt gagcgggtta
     6601 agattttgcg agaaacccgt aacgacctca atcgggctaa taatgccgag acagctaata
     6661 tcgctcggac agtttctgcc agcatgaaga ccattaacaa tatcagtaaa atcaaagata
     6721 tcatgggctt agaaaatttg ccagtagatt tgcaggaagt ggcgcaactg aggattcagc
     6781 acccagacta ctctatccag cagttagcag atagtctcag cacccctctg accaaaagtg
     6841 gtgtcaatca cagactcaga aaaatcaata agatagcgga tgaattataa cgatataaag
     6901 caaccccttg aattatcaat atcttcaagg ggttttgttg tgttatgaaa tcaaaaaggg
     6961 gcaatcaagg ggcagaatta aaaaatagta tctaatttat tgaccagttt atcctccata
     7021 tcctcagtag tatgagaata gatttccaaa gtcatttttg catttgagtg cccaactcga
     7081 tccattattg attttattgg gaggtcagac tctgctaaaa acgaaatatg agaatgccta
     7141 aaaatatggc tagataagtt tttttctatt ttggcctgtt ttccatattt ttttaatatc
     7201 tgtatgaagc aagctattgt tgtaggttga ttccattttt caaaacagaa aatataatca
     7261 tcgcttgaca atggctggaa acgttcgcta agtcgtacta tttgtctttg aatagcttct
     7321 atgacactct ctgatacttt gattgtccgt attgaatttg tagtctttgg tagcgtcttg
     7381 attttgttta ctgaatcaaa attacctgtg atctcaattt tgttgttttc gaagtctata
     7441 ttcttcagtt gtaaggcagt taactcacca tatctcatac cagttaatgt cagcacaaga
     7501 accatatcag cgtacttttg gtgatattct cgacgattaa ggacatcgac aagtgctttt
     7561 atttcttgca tggtgagaaa gttgttacgc tttttttcca gttcttctaa agtctttggt
     7621 ttttgaggaa tcgtagtata atcgacctcg ttgttttcaa tgtaagagta ttgaacagcg
     7681 taattaaaga tacctctgag cctatgccgt acttttttag ctgtaatata tccgttgctt
     7741 tcaataattt tttcaatagc ctcttgaaga aaacgcctgt caagattagc aagtatggta
     7801 tcggatggta tgacttcctt catcttctta tcaactgatt tacaattatg ttttgttgat
     7861 tcctttactg tttgcgccca tgatttatag aaaaggttat agatttcttc aaatgtaatg
     7921 ctttctactt gttttgtgct gagtttttta tttatcttct cttgtaacaa gatagcagct
     7981 tgatttcttg cctggggagt tttcttctcc atggttactg aaactttttt taatttctca
     8041 gtatatggat ctttatatcg ctcaaaatat ttaaatttgc catttggcaa ttcttccatc
     8101 cacattgcgt ttacctcact tttttgttaa aatgggtaca agaaaactac ctttttaatg
     8161 gttgtttcct atacatgatt tcctcacact ctccttggcc aaaatttgag tgtggggatt
     8221 ttttggttgc tatctcgtca gataataaaa taaggtgtac ctcttagaag tacaccagac
     8281 gattttatcc ttgacgaaca aggctttttt acattcttag tgtatttctt tttttaattt
     8341 ctgtcaagca gttatttcct aaatggaaat agttggtttt aatttatact tttggttcat
     8401 tacggtgcca atcattagga aaacctaaag actggttgat tacattgatg tctatagaag
     8461 gcagcttttt ctctagtttg ataacttttt ttctaagagt attccacaaa atgttgaatg
     8521 ctgctttact aataaagcat tgaaggctaa taattgttga atatacagtt tttctactgt
     8581 catctcccat caagatctct ttgttatgaa tcgtttccca aaacgtacta ttagaacggc
     8641 atctgaagtt taataagcga ttattgtgag cgcatttgtt tcgtacctcg ttaatatttt
     8701 ttagaaaaga tatcaaggtt tctggtggga aaacatcgtt aaagtcaggt atgtttgtgc
     8761 ttataaaact tactaaatcc cgtgcgattt cattttggag tgaattaggc aggttttcaa
     8821 taatcgtgcg taaatctcca aactctagat aatcagttag tacccagatt gggacatctg
     8881 aatgagtatg tgcgtagtgg tgaattgaat ttcctctgta tcgcttgttt gtgtttatga
     8941 ttttggatag ttttgataca ataaatccca cgtctaatat tttattatct gcataagaat
     9001 tggtgtttaa ataagcgtat ttttgactgg ggtaagcttc ggcgaatctg tgagcagtga
     9061 ttgacttgat atggtgttca gcttcaagaa tagcttgcaa aatggctcgt tttacatctt
     9121 tgtcaaatgt atagagactt gaaacttcgt caaatgtgac gccgtcaata taagtgtcag
     9181 taccagggtg ttgaaaaaac ttactgtatc cattgataat attgtagtaa ttgttactta
     9241 gcaggtactt agctgcttta gattcatctg taatggataa ccctctgatt tttagtgttg
     9301 cgatttgttc ctcgattgtt ttaaatggct tcaaaaaaac tcccccattc taatttagaa
     9361 tgaaggagtt tccccgtatc ggtccccgta gagatcccga cgctttttct ctaatgaatt
     9421 cattctaaat gattttttta aaaaagtcaa ctgtttttta atatttttaa aaaataattt
     9481 ttttattccc ctctatacac cccgactact gcataaatct tgatgtgtgt gtcttcggct
     9541 ggtgggaagt ctaggatgat gtcttcgtac ttgtcattga gtgacactag gcgtaagcgt
     9601 ccgttttcgg tatatatctt cttgaagtaa gaacggtctc catatgcgat aattgctaaa
     9661 tctccgttgt aggtagtcag tcctttgtct actaaataga gaatgtctcc gtcttggtag
     9721 tcgggttgca tggagtctcc actgaccttg gtcgcaatat cgtggcgtgg tggtcgctcg
     9781 tcaacctcta tagtctctct gtctgtatcg tcgtacccaa atccatagtt aaatccagaa
     9841 gctgctgccg tctcagatac aacctcaact tggtacaagc tgataacttc cgatacttcg
     9901 tttatcttcg tttcttcttc gttttggctc tccagaagct cctcagaggt ccgtagcacg
     9961 attttttgat tgggtggggt taattgcact accttatcca ttatctgctg tgtgagcgaa
    10021 tctggagcgt ctggcagtga gttggtggat tcttctttaa aagtagtatc tatatctgat
    10081 tttttaacac cgaaataatc agccagtttt tggataacac caaaagaagg agcgcttctt
    10141 aacttcatat agtctgtcat agtacttgct gtaattccaa cttctttagc caactctttt
    10201 tgggtgatgc cacgttgctt tctaaagtgt gtaatatttt cagcaataat ttgcattcgt
    10261 tttttttcgt ccatttacga ttacctcgta ttttttataa ttacattata tatcattttt
    10321 gaatgataaa caatcaaaaa tacgaaaaaa tcctattttt ttgataaaaa ctattgacaa
    10381 tacgaaaaaa tcgtattata attaaatcaa gcttaaggaa aaggaggtaa ggcaaatgaa
    10441 cgaactagaa agaacagccc tcaatgagat attgaggact gtgacatata tagctgagaa
    10501 gttggatgaa gtagattcta agatttcttt gaacgattca caagctcttg agcatcaagg
    10561 aaattgagtt tcatttccat gtagtgaata actccgtgta ggtagttctt taggtctgta
    10621 aaacctttat caggattatt tctatagtaa tgaccttcgt cgttaccgat ataagcagat
    10681 gcaagcgcaa atgttttaag gtcttcatcc ttgatatatt tttcaataac ttgttttaat
    10741 gacattttaa tgattttatc ttcatcatca ggatttgtaa caatggagaa atctttaaca
    10801 aagaactcaa gcgccttacg gtatccaata cctgcaatgt ggtctagttg ttcgtgttca
    10861 gctttcagtg cttggacata gatttgctta ccaattggag aaactatctc tacatcgtca
    10921 gatataggta tatcgctcgg aagattaggg attactttga tatgttcaat ttcgtatttt
    10981 tcggtatagt cattgattaa aaatcttgta gctataaact cctctgtcca aaagtgctta
    11041 caacctaagc atctacatgt taatactaag cttgttttat tttctccaag agaaaaataa
    11101 gaagagttca caagatctgg attggttggt tttttacaat ttgggcaaat gtcttcaacg
    11161 gttacaggcc tagaaacaga agaatttact ttcactcgaa atatcataag atttctccaa
    11221 tcgttttatt ttgattatac catatttgaa agggggtgag gaaatgagac caagacgata
    11281 tccgtatagt gggaaaaaag agtccacctt cgtaaaggca gaccctgagt taatgttgaa
    11341 tatcaataag attgatgttg gaaatataca agcgaaaaat atatttggaa aaatttaagg
    11401 agtaggagga tggatgaaag aaaatataga acatcttcat gaacgcataa agcattttca
    11461 gtcattgatt gcttatatgt ctgagcatga gaaaaagtat tatttagaaa aagattggtt
    11521 cgataaccca accctaattt ctatagaaga cgcaaaaaaa gaagtagaac tactttaaca
    11581 gaaaggaggt aggaacgtgc agtggacttt agaagctatg agaatcaaca aaggacttac
    11641 tcaagcagag ttggcagaaa aatttgaagt ttcaagtcaa acaattgcta gattagaaaa
    11701 agatagctct gatatcggtt atcagctatt gaaaaaatac atgttttttt tcaatgtgaa
    11761 attcgatgat atttttttag ggaaaaaata cgaaaatttc gtaaataact agaaacaaaa
    11821 aaataagaag gaggaacaca tagatggaaa gcgttcttga aagcttgaaa caagaaaaag
    11881 accacctcga aaaaatcatt aaggtagtta cctctggtgg taaatttctg agattgccgt
    11941 atcaaaaaaa gtcacgctcg attagtgaga atctgaaatt gatttctcaa aatcttgata
    12001 aattgagcga gcaagttcaa caaaccacga atcagcattc atgatttcaa gatgacgaaa
    12061 gaaacctgtt ttgatttcca gtttgatatc atgttacagc gactcgataa agcgattagt
    12121 gattaaattt ttgagatgta agtttttttc atgtcaaact ttttcatgtc aaatttttta
    12181 cactaataaa tattaactaa caacaagtat taaaagacaa ccagtcctac ttctctaaat
    12241 aaaagagagg gtagaaaaaa taaatacaaa ggaggtgagg aaatgagacc aagacgatat
    12301 ccgtatagtg ggaaaaaaga gtccaccttt gtaaaggcag accctgagtt agttgaaaaa
    12361 cttttaagaa acactagttt tcttgagcgt ttacaaaaaa agcctatcaa ttttcagata
    12421 gactcagaag aatttaagcg tcttagctat gaagccattc atgatacttc tcaagtaact
    12481 caatagtagt tattacagaa gtcaaaccac tgaccttccc cagttgcaat ccgtctgtat
    12541 ggtcaatctg tttagtagct tcattagctt tagcagagat agcttgcata tcttcagctg
    12601 ttaaagattc tcgaaaatct ttaaaggatt tcataaaaac tcctcctttc tattggaatt
    12661 ttgactaaaa cggtgagagg tcctagtcga gattattata gcaatttagg aggatattac
    12721 atcggtcttg aggctgattt ttggaggcaa tattggaaga taaaatcata gaacttgctg
    12781 attacttcat cagcgaatct acaacgtaca gagaagcaaa aatagcgtgt gagaagctat
    12841 tgaaacaagt cagccacgag attgaactca gggcgatgga aagtaggaca gtatgaaaga
    12901 agcagtaaag gaatttctaa aattcaggag ccgatttaca aaaatagaat ggtttgaaat
    12961 caaccaagct gtcgaagctc gtttaaatca aaaagccgac cagttgaaac tggacgacgt
    13021 agatttagaa atcatttcta gcagactaga aaaagttatc tagaaacgat ttgaatgaac
    13081 attggatgga tacgatagtc agcgccacga tagtgaatgt agatataatc ctgatggtac
    13141 atcgagtttg cttcaggttt agaaattggt gagtagagtt ctgcattttc ttcccaccaa
    13201 atgtaaggac tagccatatt tggtcccatt acacaatcgt cgtcggctga taggttcacc
    13261 caatttccgc aaagacatgc gtgaatttca gtcataatat tacctccttt ctggtttcat
    13321 tatagcagaa aaggagatag caaaaaagca cctgacggaa atcaggcgca tacttaaata
    13381 ttcaacatga ttataacacg aaaggagcaa aaatggaagc agttgaaatt gtaagaatta
    13441 aagatgtgat tattgaaaaa gtctctgcta atgatgaaga gttaaaacgt atctttggat
    13501 gttcaaaacg acaagcagga gagcgaagaa gagaaatgca aaaactccct agtcagcaaa
    13561 aacatctttt ggatagtgga caacttgtaa cgattaaagg tttctatgaa tacttgcaat
    13621 atcgtggaac taaagcttgg aaaaaagaaa tggaaacaag caagaaaatg aggtcagcag
    13681 gatgaaccta ctatcaagaa tcaaaaacta tttttcggaa gaggtcaaag aaactaatct
    13741 cgactggaaa gaggtcgctt tagacctcaa tcaatcacta attgaaacac aggaaaaact
    13801 tcaagaagcg aatcaagaaa tcgcagactt gaagaaaatc gtagcaatct ataaagaaaa
    13861 ggagaaagaa aaacgatgga atatatttac ctggtaacaa tcgtaggaat tggactatgg
    13921 tcgctagtaa ataaactaga tgaccacgct gaaatgaaac aaaaagagcg ccagctgatg
    13981 gcaaacaatg tcgcacggat gaatctgaga aattcagata agcaatttac ttatgatgta
    14041 gaaccgcctg aagggttgaa ataaggagga gaaacatgac tcaagctgaa cgaattaggg
    14101 aatattatag agagcaccct gctgcctcat atgatgaagt ggctgaggtc gttggtacaa
    14161 caaatagtaa tgtgagagcg aacctggcca aagacatcaa ggcaggcaga tgcgttcgct
    14221 tggaagataa gtcatacgat tactcgcctt actataacca tacacaggca ctcactgagt
    14281 tggttgactg gaagaatgac aacagacgtg agtgggtgga tatgctgaca agagcagcag
    14341 agaaagaaac ggatagcaac gttatgcgtt tgttaatcaa agaagcaaat aaattgatga
    14401 aagaggtgac taagtagatg gtacgaaata aaataggtga tttaactaac acgctcttcg
    14461 ctcaattaga gactctggat gatagggatc ttacagcaga tgaattaaaa gtagaattac
    14521 accgctcgaa acaaatggtc gcaatctcag gtcaaatctt acaagcaggt caattggcgc
    14581 tagatgctga aaaattcaaa gacaaggtag gtgaagtcaa tgccccgatc gctttgctgg
    14641 aaggatgagt acacggagta catgcatgaa atatgccctg gtcgtttaac tcctgaagta
    14701 accaggttgc tgaatgaaaa atttggtacg aattataaca agagtcaaat cggtggcgta
    14761 cgcaaacgtc tagggttagc agttggaaaa gtctatcaag gtcgattgct gacaaaggag
    14821 caacatgatt atcttgtatt gatccaaaaa aataagattt ctcgtaatgt cgcaaatgaa
    14881 atgaaccaaa aatttggctt atcgctaact gagaaacaga ttaagagtta tcggagaaat
    14941 aataatctac atagtggttt gacaggaaga ttcgagaaag gtcagactcc tcacaataag
    15001 gggaagaagt accccaatat gccaaaaaac ggcgggcagt tcaaaaaagg taatcgacct
    15061 ccgaattatg tacctgtcgg tactatcaac tacacaacaa acggttatcc aaaagaaaag
    15121 attggagaac ctaatcaatg ggttttgaaa caccgcaagg tttgggagga ccatcacggg
    15181 ctgataccaa aagggtactc aatcgttttt ctggacggtg ataaaacaaa ctatgatatt
    15241 tcaaatctgg catgtttatc taagaacgaa attgctagaa tgaatcaaaa tcatttattc
    15301 acgtccaacg ctgatttgac taaatcaggt attggactaa caaaacttac aaataaaatc
    15361 agagaggtag aaaaaaatgg ctagtttata cgaactaaca ggtcagttcc tgacaattta
    15421 ccaattggat atcgatgacg aaacaaaagc agacacgctt gaggccatcg attggcaaga
    15481 acaatttgaa cagaaagcag aaggatatgc ccatgttatc aagaatctag aagccgacgt
    15541 ggcaatgtac aaggctgagg aagagagctt caaagccaag aaacaggtgg cacagaaaaa
    15601 gctggattat gtcaaggata acattatggc agctatgaat gtcacggggc aaaccgaagt
    15661 taagagtggt gccctgatta taaaaattgc taagaatcca gaatcagtca aggtcaacga
    15721 agacgacctt ccgaaaaaat attttacaaa aaaagtgacg cttgcgccgg acaaaaaaac
    15781 actcaaagag ttgcttaaat ctggcaagaa agtcaaaggt gcggagcttg tccggacaga
    15841 aaagttggtg attaagtaat ggaattgatg aataaaacac gagtaacaga ttcactagca
    15901 gttgtgattg gaccagaatc gattgaagta cttgttactg aaggttttct atttgatgtt
    15961 gcgattcgtt ttgtaaaagt agacgaaaca aatcttgatc aaggaaatga aaagccagta
    16021 ttcactccgg aatacaagct ggtcacagtt gctaaataca aggaaaaacc tatctttgaa
    16081 tcggaggaag atattcgaaa atttgagaag caagcaaaag aagttaaatc gctatttgcc
    16141 tttgcaaagg taaataaaca aaattggttt aacactgccc tttatccagg agtgctgact
    16201 gagaaagttg gtgtttgatg aaaattttag ctattgatcc aagtagtaat aaaattgaaa
    16261 ccagcacaac aggagttgtc ttgttggata atgcaagatt agttgatagc tgggttgtct
    16321 cttatggtat gagaggtttc gctgattggt ttcacgaaat cggaacaaat cttgaattcg
    16381 atgtagttat tgttgaagaa tttaaggcga gggataacga caagtcgaaa gataatagcg
    16441 tggcagaaac catcgcctat atccaacttt gctatccagg tgccattctt caattcaatg
    16501 caggttacaa gtcggatatt ccaaacgatc ttttgaaaat cttagacctt tggaaatttg
    16561 aaaaaagtca tcatcaagat attcgagcag cagcaagact tggattattt tgggcaatga
    16621 gaaatgatat tgaagaagtg gttcatgata tcggaaaggt ggtgagtgag tatcacaata
    16681 acgctaagaa agtggcaagc tgaagcgatt aaaagaagtg aacatttatc taatggaatc
    16741 tttttagagg ctcttggggg cagaggcaaa actatctgtg cacttgctat tgcaaaacat
    16801 aaaaaagcta aaaaaatcat catcacaaac aatcgactag ctattctgaa tggttggata
    16861 gatgcagtca agtttatgaa ttttgataaa ggtgttgaga ttatcattca gacagataga
    16921 tatcttcaaa atcaagtcaa aaaggggcat aaattagatt gtgatgtgct gatagtagac
    16981 gaatggcaga atatgtcttc tgacaaacaa gtggccttat atcgcaaaat aaagcgaaaa
    17041 tacacgatag gtctttcagc gacaccaatt cggaaaaaag gacaaaattt ctatccgctt
    17101 gaaaaaacgg tatttggttg ggcaacccca aataataaat ttgactggca aaagactcat
    17161 ggaaaaatgg tctatgatcc atttagctat tcaaaagaga agtgggaaga ttttcaaaat
    17221 tatgaaagtt atatctcgag cttgcctaat ttcttccgct gggaagagat tgaaggaatt
    17281 gagaatgcag ttgagaataa cggttttgag attaagtttt accaaaagag agtcgcctct
    17341 ggcaatccag aaaaacttgc agaatttaga aaactaaatc ttgtaacagt ggacggcaaa
    17401 actgcaatgg ccaagcaatc gtttggaaga aagacctttg aacgctacct taatcaaaca
    17461 ggcgtagcag tcgattttcc aaaattaaag ccagtaaatg cggatacgcc attgatgtta
    17521 caacttgacg gtttaatcga acgagcacca cacgatatgt tgattgtcag taaatctaag
    17581 cagattgtca acgtcattag cgagcgccat cctgaaattg gaatctggac gggcgatatt
    17641 caagaaggac tttataagaa attcgtggtt gctactagtc aagtgttagg tgtcggagta
    17701 gacggcttgc aacacaaata ccaaactatt gtcgtattgg atccagtaga agaaggttct
    17761 ggagaatatg atgattatcg acaattgctc tggcgcataa caggaagtcg tcagcagcat
    17821 gatgtaaatg taattgaatt ttattataaa gaaagttaaa aaaagaggaa aacaaaatga
    17881 ataaaacaac tgaaatgatc gtatttcgta gccgtaaaac tggagaattt cttaattctt
    17941 acaaggacag aagttcttta gcatttgcag ctgacttttg cagcttggaa tattgtttga
    18001 agcttcctcg taaaaaatac gaagacaaca aaaagactta caaggctctt gctgcagctt
    18061 ttgactgtga aattgtcgca gttgaagcgg aatacaaatt gacctatccg aatggatcag
    18121 aagttgaacc tatcaagcgt gaccgttcat caattgagga catgattaag gatattattg
    18181 gaggggttct ctaatggcat ttacacttcc agcaaataaa ccacaagttc ctaaagatac
    18241 cccacgaaat tttttcatct acggcgaaac catgagcgga aagtcttatc ttgcaaatga
    18301 attcccaaat ccaatcgttt tgaacacaga cgggaatgca gaagctaaca ctgttccaag
    18361 cattcagctg atcaatgaaa aagatgacaa gggacgaatt accaattcag taattaagca
    18421 gcttggagat atcttgcttg ctctccagac acagaagcac tcttatgaaa cagtcgttat
    18481 tgatgtaatt gacgatgtta ttgaaatgat taagattgca gtttgtgatg aattaacccc
    18541 agttggtaaa cctcgcttga aatccttgtc ggaaattcca tacggcaaag gatacgactt
    18601 ctttaaccaa gctatcacag aattagtcat tgacctcaaa gcattgccaa tgaatgttat
    18661 ttacatcagc cgtcaggtat ctgaatatga tgacaatggc aatgccacca aagacaagcc
    18721 aagcttgaaa gataagtatg tcaatcttat caatggaaac tctgatttga tgatccacac
    18781 tgaaaaactc ggcaacaact acaaccgtga ggttgaccgc aagcgtaaga cctattatgc
    18841 ggaccaggtt gatgacaagg ccatcttgaa aatcttggca actatccgtg gggctgttga
    18901 gcctgcaaag ggcaagctag cccctaaaaa agaagcagct aagacaacta aaccagctaa
    18961 gaccgaaaaa acaaaagagg cacctaagaa agaagttgac tctgatgatg aactatttta
    19021 agaaataaag gagaatacac atgagcttac tagatatcgc aaaatcaatc aaaaaagagg
    19081 gctttgaccc acgcaaagac agcgccaacg gtcctgcacc aatcccagct ggtacttatc
    19141 cagtagtcct gaagaaagca accttcaacg tatcggacaa aggctgggaa agccttggtt
    19201 atcaatttga aatccgtggc ggtgattaca gtggacgctc tgaatttgca acatttggca
    19261 cactgactga atggaacggt aagaaccttg actgggcagt tgaacgcact atgaaattct
    19321 ttatcaaagc cttggtcctt gctggcgaca gtatgcaagg aaatgaagaa gacggtaaag
    19381 ccttggaaga ggctctacaa cgtaaggcag ttggctctta ctacaacctt gttatctctg
    19441 tgactaaggg gaaagatggc cgtgagtttc gaaactatga ccttgaagaa gaagaagcac
    19501 aaccgctgac tgaagctgat atcgatgaag atgacctccc tttctaaaaa atagcaagtt
    19561 ttgggtcatt gatgaaactg atgaaagatt aggaccattc aatacatttg aagaggctta
    19621 tcaatcgttg ttattttatt taaaaatgac tgaagatgaa tatcagtcaa actatacggc
    19681 ccaggaactt gtttatattt acaaagagga gaaaaaacca tgccgtcgat gaaagaatac
    19741 gcattacagt accaaaagtt agggttctca gtcattccaa tcaatcctaa aaacaagatg
    19801 cctttaatcg aatttgctga taaaccagcc atgactccat ctgagattga aaacttttgg
    19861 gacggctacc ctaatgcaaa cattgcccta aagactacca acttctttgt cattgatatt
    19921 gacaaacacg gcaaatcgaa cggttttgaa tcgctaaaaa aatggaaaca tctaaattta
    19981 atcgaaccga cactgcaagc taagacggct agtggcggta aacatctatt ctacttcaaa
    20041 cgagaagatg agccgatcac tcagatgatt ggattcttgc ctggtgttga tattaaggct
    20101 cacgaaaata attatgtgtt agtcgcaccc tctgccacag ataaagggca gtatgagtgg
    20161 gatctggaaa agtctaagga aggtggcacg atggtcactc cttcaaaata tttaatccag
    20221 tctataaaaa aacagtatgg cgaaactcac ggttataagt atgatggtaa ggacggtctt
    20281 agggatttag ttagacgttc acatactaga gaccgaacac agactacaga tctctttgaa
    20341 accatcgccc ttggttttgg tgatgaaggt ggacgaaatg acaaactagc aaaattcgta
    20401 ggtggtctct tatatcgtgc ggtcgacgat ggtgtagttg ttcaacttgc aagattagca
    20461 aatgcaaata gtccaacccc tttgcctgaa aaggaaatga tgcgtactat tgaaagtatg
    20521 attaaaaaag ataggaggtg attgtgattg gtaatgtagt aagtattgac tcacaaccta
    20581 agatgataac gactgccaag ggagacatca aggccaacag tccaagtaat gtgttgatgt
    20641 ctttcaaagc tgatgatcag ttgagtattt acctaaagca caacgatttt tcccaagagc
    20701 atgaactcct taaagatatc aagatcggca acactctttt taaaaaaggt gagctccctt
    20761 ctaactttga ttcagtcgta aaagtttact ttgaaagtgt gttaggtgtt gctttctcta
    20821 gccaggcgat gctggacggc atggaaactt ttttctcaga acgttcttac aatccagtca
    20881 ttgagtatat ggaaaaggct gctgaaaaat gggatggtcg taaacgaatt gaccgaatgc
    20941 ttcaggttta cctgggggct gaagatatcc ctttagtttc taaaatcgct caaatgtggc
    21001 tagttggtgc agttgctaaa gtttatgatc catacgttaa gtttgactat gttctggatc
    21061 tagtcggtgg acaaggagtt gggaaaacat ccctccttca aaaattgggt ggcgaatggt
    21121 atacggatgc cgtaacagat ttctctaata aagataatta cgacattatg ttaaagagtc
    21181 taatcgtcaa cgacgacgaa atggtggcca gtaatcggat gagctttgca gaaactaagg
    21241 cctttatttc taaaactagc ctacgttatc gtaaaccata catgaaacgc acagaagagt
    21301 ttgccaaaaa cttcatttta gctagaacta ctaaccaaac agaatacctc aaggacaaaa
    21361 ccggtgaacg tcgttttcta ccagttatgg cagatagtaa acggcaaaaa aaacatccaa
    21421 tggaaatcga gccagagaca attgaacaaa tctggggcga agccgttaca atctatcgtg
    21481 ctggtgctga tttgatgttt gatgaaaata cagaggatga actgaatatc taccgtgaac
    21541 agttcatgta tcgtgatgaa gttgaattac aagtgcttga atatcttgat atgcccgtcc
    21601 ctgaaaattg gcaaaactgg tctattcagc aacaacatca atacacaagt aaatatttcg
    21661 ataatagtag cgactttgat cctggaagca aaaaactaga taaggtctca actcgtgaaa
    21721 tgatgtacaa cttatttatg agaaattcga atgacaggaa gctgtcaacg aagattaaca
    21781 tgatcatgga taatcatcct gattggaaaa aaagtgtttt ccgggcagga ggtaaaagta
    21841 caaaagggtt cgtaagagtg aaagattcgg aaaaaactaa tcggtagcaa ttaaaaaatt
    21901 atcggtagtc atcggtagca gttgaggggg agatcggtag cattctaccg ataaaatagg
    21961 acatcggtag cacatcggta gcagtctaac cccttgatat tactgacttt tatttaatat
    22021 ttatatataa tgctactctt ctacctatat ttttaaaaaa agtatataaa ataatagtaa
    22081 taataaagaa agcctataga atagggattc ttgaaaaaaa ctttttactt tttagaattt
    22141 atcggtagca cggtagcagt ttagaaaaaa gaggtaaaaa tgtcatacac agtaacatta
    22201 ttttttgaca acatggtaga cgaaactcac ttttttaaga aagagagtga tgctgccaaa
    22261 tgcaaggctc agttagaaag caagtatcga gggaatcgaa tgtataaagt taagcaggag
    22321 aaattggaag aatgaataag caggaactga ttaaacgtat cgaggatttg ccttatacag
    22381 aggggcctat cgcagataca atcgaaatta atagaaattg gatattgaaa tcaattgaac
    22441 agctagccga atccgaaata ggtcacgcag atgaagctcc acgctacgta aagaacatac
    22501 tagcacgatt gcgagaattg ccattgcatg atagagagtt ttggttaaag gctatcatga
    22561 gcgaatttga acaggatttt agccatgcaa aatggcgaga gggctacgag caaggtaaaa
    22621 ttgagggtat ggttgaacgt gaaaaagtca tagttccgca gtgtgtggcg gaatatatag
    22681 aatttaaaaa gaaaaacaat tttcatgttt acggtgcaat gagagtaatt gaagatcatt
    22741 atgataagaa agttcctga
//
LOCUS       DAVKQB010000035        22551 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKQB010000035 DAVKQB010000000
VERSION     DAVKQB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.035"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            110..763
                     /locus_tag="VW528_001412"
     CDS             110..763
                     /locus_tag="VW528_001412"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9912223.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            773..1237
                     /locus_tag="VW528_001413"
     CDS             773..1237
                     /locus_tag="VW528_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9912224.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1250..2518
                     /locus_tag="VW528_001414"
     CDS             1250..2518
                     /locus_tag="VW528_001414"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9912225.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2532..2792
                     /locus_tag="VW528_001415"
     CDS             2532..2792
                     /locus_tag="VW528_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912226.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2803..4164
                     /gene="pdrM"
                     /locus_tag="VW528_001416"
     CDS             2803..4164
                     /gene="pdrM"
                     /locus_tag="VW528_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9912227.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4318..5286
                     /locus_tag="VW528_001417"
     CDS             4318..5286
                     /locus_tag="VW528_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9912228.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGSVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5302..6294
                     /locus_tag="VW528_001418"
     CDS             5302..6294
                     /locus_tag="VW528_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9912229.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEEISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCSGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6512..7555
                     /locus_tag="VW528_001419"
     CDS             6512..7555
                     /locus_tag="VW528_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9912230.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7619..9304
                     /gene="lpdA"
                     /locus_tag="VW528_001420"
     CDS             7619..9304
                     /gene="lpdA"
                     /locus_tag="VW528_001420"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9912231.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9368..10357
                     /locus_tag="VW528_001421"
     CDS             9368..10357
                     /locus_tag="VW528_001421"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9912232.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEAVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10430..11500)
                     /gene="xerS"
                     /locus_tag="VW528_001422"
     CDS             complement(10430..11500)
                     /gene="xerS"
                     /locus_tag="VW528_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9912233.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
     gene            complement(12008..13558)
                     /locus_tag="VW528_001423"
     CDS             complement(12008..13558)
                     /locus_tag="VW528_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9912234.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(13574..14353)
                     /locus_tag="VW528_001424"
     CDS             complement(13574..14353)
                     /locus_tag="VW528_001424"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9912235.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKEN"
     gene            complement(14340..15191)
                     /gene="ylqF"
                     /locus_tag="VW528_001425"
     CDS             complement(14340..15191)
                     /gene="ylqF"
                     /locus_tag="VW528_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9912236.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWCQYFESQEIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(15437..20695)
                     /locus_tag="VW528_001426"
     CDS             complement(15437..20695)
                     /locus_tag="VW528_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9912237.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVIGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVQPALPEAVVTDKGEPEVQPTLPEAVVTDKG
                     EPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEP
                     ISTVSEVASEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVKEVGEDGERQIVT
                     SYEELHGKKISEPVETVTILKEMKPKILVKGTKENPKEKTVPVLTLTKVTEDAMNRSA
                     NLNYELDNKDNAEISSIIAEIKDGDTVVKKVDLSKEKLTDAVQNLDLFKDYKIATTMI
                     YDRGQGSETSKLDEKTLRLELKKVEIKNISSTNLVKVNDDGDDGTEIPSDFMSEKPSD
                     EDVKKMYLKITSRDNKVTRLAVDKIELVTEKEKELYKITASAQDLIQHVDPSKTRNEY
                     IHYIEKPVPKVNNVYYNFNELVRDMQEHPNDEFKLGADLNATNVSAFGKSYVTKDFKG
                     KLLSDGDNHYTIHNLSRPLFGNVIGGTIKNINLGNVDINMPWANQVAAVANIIKGGTT
                     IENVKVKGNIVGKDWVSGFIDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKG
                     LVERAYVNANLIGKKAKAAGIAYWTQNEGNNNTVRQEGAIKKSIAKGTIQVTEAIESG
                     GVVGSMKHHGSVEDSVSMMKVPNGEIFYGSSDIDYDDGYWTGDNVRRNYVVIGVSDGH
                     SSYQRSKDKNRIRPISEEEAKSKIEATGITADKYEINEPVVNRLNRLTRREDEYKSTQ
                     DYKVDRDLAYRNIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDL
                     SDIDHVMIHYADKTKEIKAVHQKESKVAQVREYSIDGLDDIVYTPNMVDKNRDQLIKD
                     IKDRLATVELISPEVRALMDKRDTSRDPNANSDERKNGYIRDLYFEESFSETKANLDK
                     LVKSLIENADHQLNSDEAAMKALVKKVDENKAKIVMALTYLNRYYDIKYGDMTIKNLM
                     MFKPDFYGKSVDLLDFLIRIGSSERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMR
                     LFTNDTDLNDWFIHAAKNVYIVEPKTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLK
                     NAHMFLISTYNTMAYSSFEKYGKYTEAEREAFKDKIKEVAHAQQTYLDFWSRLALPSV
                     RDQLLKSQNRVPTPVWDNQNYHNVEGVNRMGYDKNNKPIAPIRELYGPTWRYHTTNWY
                     MGAMASIFQDPNNNDQVYFMGTNMISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDV
                     EAYAQGMLQTPDKSGNGEYGALGLNMAYHRENDGDQWYNYDPDKLKTREDIDRYMRNY
                     NDALMMLDHLEADAVIPKLHGNISRWFKKMDRQYRKNGELHQFDKVRELTEDEKKKIV
                     INNIDDLVNNNLMTKHGAPSDRTYNPEDFDSAYVNINIMTGIYGGNTSQGAPGAASFK
                     HNTFRMWGYFGYENGFISYASSKYQGEADKTNKKLLGDDFIIKKVSKDKFNNLEEWKK
                     QYFKDVKSKAEKGFTAIEIDGRQITNYAQLKTLFAEAVQKDIDGMSDPKIKDHFKNTV
                     DLKSKVFKALLKNTDGFFNKLFKEDI"
     gene            complement(20739..>22551)
                     /locus_tag="VW528_001427"
     CDS             complement(20739..>22551)
                     /locus_tag="VW528_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9912238.1"
                     /translation="KSGFNNLLAKNNVITYNVLLAKNYGTESLFKALEGYRKVFLPTI
                     SNNEWFKKQTKAYIVEEKSTIEEGREKQGKEGTKYSIGVYDRLTNPSWKYQSMVLPLL
                     TLPEEKTVFMIANISTIGFGAYDRYRSSEYPKGEKLNKFVEDNAKEAAKRFRDHYDYW
                     YKILDNDNKEKLYRSILVYDAFKFGTDKDKDKVTHQATFETDHPAIKYFFGPAGNNVV
                     HNGHGAYATGDAFYYMAYRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRSGLGPEFYA
                     KGLLQAPDHPYDPTITINSVLKYEDSENSTRLQVADPTQRFNSAEDLHNYMHNMFDVI
                     YMLEYLEGKAVANLETNQKYELLRKIENKFDLDQDGNNVYATNVVRRLTMDEVNKLNS
                     FDSLIENDIITSRGYKDQEYKRNGYYTIDLFSPIYSALSGEKGTPGDLMGRRIAFELL
                     AAKGYKEGMVPYISNQYEKDAKAAGSKINSYGKEVGLVTDELVLEKVFNGQYKTWTQF
                     KKDMYKEREKQFSKLNRVNFINPNNPLSRQRNVSVTDIGVLERMIVEAVRDDAQDDVA
                     KFYPETNSRVLKLKKAIYKAYLDQTNDFRSSIFENKK"
BASE COUNT         6628 a         4390 c         4353 g         7180 t
ORIGIN      
        1 tcaaatcgta tatatagtcg atttattaaa tctttagata aactgtaaaa agtggtttcc
       61 atagccactt tttgctataa tagaggcagt aaaaatgaag gagtcggcta tggaacactc
      121 gtcttggcat gctttgatta aggcgcaatt acctgagggt tatttcggga aaatcaatca
      181 gtttatggag caggtctatt ctcaggggat tatttatcca cccaaggaaa aggtttttca
      241 ggctctcttg acaacactgc ttgaagaagt taaggtggta attctagggc aagaccccta
      301 tcacggacca ggtcaagcgc agggcttgag tttttctgta cctgactcta tcccagctcc
      361 accatccttg caaaatatct tgaaagaatt gtcagatgat atcggagtta agaaatctca
      421 tgatttgaca gcttgggctg agcaaggagt cttgcttctt aatgcttgtt tgactgttcc
      481 tgctggacag gccaatggtc atgctggtca aatctgggag ccttttactg atgctgtgat
      541 tcaggtggtc aatcatctag atagaccagt cgtttttgta ctctggggag cttatgcacg
      601 taagaagaag gcattagtta ccaatcctca tcacttgatt atcgaatcag cccatccaag
      661 tcctttgtcg gtttatagag gattttgggg ttccaagccc ttttccaagg ccaatgcatt
      721 cttaaaagag acaggacaag agccaatcga ttggcttaga taaggagaaa atatgcctca
      781 gttagcgacg atttgctaca ttgataatgg gaaagaactg ctcatgctcc atcgtaataa
      841 gaaacccaat gatgtccatg aagggaaatg gattggtgtg ggtggtaagc tagagagagg
      901 agagacgccc caggaatgcg cggcgcgtga aatccttgaa gaaacagggc tcaaagccaa
      961 gccagttcta aaaggtgtca tcacttttcc tgaatttaca ccagatttag actggtacac
     1021 ctatgttttt aaggtgacgg agtttgaggg cgacttgatt gactgcaatg aggggacgct
     1081 agaatgggtt ccctatgatg aggttttgag caagccgact tgggaaggtg accacacctt
     1141 tgttgagtgg cttttagagg ataaaccctt cttttcagcc aagtttgttt atgatgggga
     1201 taaattgttg gatacccaag ttgatttcta tgaataaagg agaaagcaga tgctactaat
     1261 caaaaatggt cgtgtaatgg atcccaagtc tggtttggat caagtttgtg atgtcttagt
     1321 tcaagatggg aaaattatca aaattgcgtc tgagatcacg gaagaaggag cagaaacgat
     1381 tgatgctact ggtcttgtgg ttgctcctgg cttggtcgat attcatgttc atttccgtga
     1441 acctggtcaa acacataaag aagacattca tactggtgcc ctagcagccg ctgcaggtgg
     1501 ttttactact gtcgtcatga tggctaatac cagtccaacc atttcagacg tggagacttt
     1561 gcaagcagtt ctccagtcag ctgccaaaga gaagattaat gtcaagacag ttgcgaccat
     1621 tactaaaaac tttaatggta aaaacttgac tgactttaag gcactcttag aagctggtgc
     1681 ggttggtttc tctgatgacg gtattccgct tgagagcagt aagattgtca aggaagccat
     1741 ggaggaagcc aaaaaactca atacctttat cagccttcat gaggaagatc caggtttgaa
     1801 cggtgttctt ggctttaatg aaaatattgc tagagaacat ttccatatct gcggtgctac
     1861 tggggtggct gagtacgcta tgatggcgcg tgatgtcatg attgcctatg caactaaagc
     1921 ccatgttcac atccagcatt tgtctaagga agaaagtgtt aaagtagtgg agtttgctca
     1981 ggggttaggt gcagaagtca cagcagaagt agcgccacag catttctcta agacagaagc
     2041 acttctttta acacaaggta gcaatgctaa gatgaatcca ccgcttcgtt tggaatcaga
     2101 ccgtcgtgcc gttatcgaag gtctcaaatc aggtgtcatc acagttattg cgactgacca
     2161 cgcgcctcat catgtagatg aaaaaaatgt tgaggatatt accaaagcgc catctggtat
     2221 gactggctta gaaacatccc tgtctctcag cttgacctat ttagtagaag ctggtgagtt
     2281 gagcttgatg gaattacttg aaaaaatgac atacaaccca gccaagcttt acaactttga
     2341 agcaggttac ttggctgaga atggtccagc agatatcact atttttgatg ccaaggctga
     2401 ccgctttgtg gactcccatt ttgcttccaa agcagctaat tcaccattca tcggtgaaac
     2461 cttaaaaggg caggttaaat ataccatctg taagggacaa atcgtctatc aagcttgata
     2521 ggagttagaa tatgaattat tttcgaaaac gtagggagag acaagcgaaa tctaatagtg
     2581 gaatttactg tcccgcagct aatcgttcca aaattcaata tgaaactaaa gaaaaagctg
     2641 accatgcggt tcaatatgat aacggaggtt tagttagaag ctattattgt cgtacatgtg
     2701 cgtgttggca tactacttca aaatctaata aacctccgct aagtttgaaa aactatggtc
     2761 tgaaactctt tggtttagat aaatcagaat agaaagagaa gtatgtataa gacaaagtgt
     2821 ttacgagaga agttagtatt atttttaaaa attttcttcc caatcctgat ctaccaattt
     2881 gccaattatt ctgcctcttt tgttgatact gcaatgacag gtcaatacaa cactatggac
     2941 ttggctggtg tatctatggc aaccagtatc tggaatcctt tctttacatt tctaacaggg
     3001 attgtgtcag ccttggtgcc tatcattggt caccatcttg gtcgaggcaa aaaggaagaa
     3061 gttgcgtctg atttttacca atttatttat ttggccttgg gcctatctgt ggtcttgctg
     3121 gggatggtac ttttcttggc accaataatc ttgaatcata ttgggttaga agcagcagta
     3181 gcggcagtag cggttcgcta tctttggttt ttatctatcg ggattatccc cttgttgctc
     3241 tttagcgtca ttcgttcctt gctggattcg ctgggcttga ccaaactgtc catgtacctc
     3301 atgcttttgt tactccctct caatagcgga tttaactatc tcttgattta cggtgccttt
     3361 ggtgttccag aactgggagg ggctggtgct ggtttaggaa catccttggc ctactgggtc
     3421 ttgcttggga tttctgttct ggttttattt aaacaggaga agctcaaagc cttacacctt
     3481 gagaaacgaa ttccacttaa tatggataac attaaggaag gagttcgttt aggtctgcct
     3541 attgggggaa ctgtcttcgc ggaagtggct atcttttcag tggttggctt gattatggct
     3601 aagttttcgt ccttgattat agctagtcac cagtcagcta tgaacttttc aagtcttatg
     3661 tacgcctttc ctatgagtat ctcatcggct atggctattg tcgtttccta tgaagtggga
     3721 gccaagcgat ttgatgatgc gaaaacctat attggtctag gaagatggac tgctctcatt
     3781 tttgcggcct tcaccctaac cttcctttac atttttaggg gaaatgtggc cagtctttat
     3841 ggtaacgacc caaaatttat cgatttgaca gcgcgttttt taacttatag tcttttcttc
     3901 cagttagcag atacctttgc ggcaccgctt cagggaattt tgcgggggta taaggataca
     3961 gttattcctt tttaccttgg tttgcttggt tattggggcg tagcaatccc tgtggctacg
     4021 ctatttgatt ccctaacaga ttttggagct tattcttact ggatcggctt gattattagt
     4081 ttgattgtga gtggggcgct ctaccgttgg cgtttaactg tgattatgaa gagatttgaa
     4141 tctttagcaa aatccaaacg ctaaaaagtt tgtgaaaaaa tattcttgac aagaaaatcc
     4201 cttgttttca cggggttttc ttttttattt tgtgaaattt cttttagaaa aactttgaca
     4261 gtatttgcca aaagcggtaa aatagtaagg taagaagaaa gttagaaagg caagaagatg
     4321 tcaactttag ataaaaatct tttgctagaa atgttccgta agatggaaga aatccgtcgc
     4381 atggacttaa aaattgcaca attagtaaag aaagggaaag tgccaggaat gacgcacttt
     4441 tctgttggtg aagaggcagc taacgtgggg gctatgttag ctctcaatcc agatgatctg
     4501 attacctcaa accaccgtgg acacgggcaa gctattgcta aagggattga cctcaacgga
     4561 atgatggctg aaatccttgg taaatacact ggaacctgta aagggaaagg tggatctatg
     4621 catatcgctg accttgatgc tgggaacctt ggtgccaatg gtatcgtagg tggtggtatg
     4681 gggatcgctg tcggtgcagc cctcagtcag caaatgcaaa ataccggtaa aatcgttgtc
     4741 tgcttctttg gagatggtgc gaccaatgaa ggtgttttcc acgaagcagt gaacatggct
     4801 tctatctgga acctgccagt cattttctat tgcattaaca acggttacgg tatctctgcg
     4861 gatatcaaga aaatgaccaa tatagaacat atccatcaac gtagcgccgc ttatggaatt
     4921 cctggaatgt tcatcgaaga tggtaacaat gtcatcgatg tctatgaagg atttcagaaa
     4981 gctgtggacc atgttcgcag tggcaatggt tcagtcttga ttgaaagtgt aacttatcgc
     5041 tggctcggtc actcatcatc tgaccctggt aaatatcgta cgcgtgaaga agtggaattg
     5101 tggaaacaaa aagatccaat cgaaaacctc cgcaattacc ttattgaaaa taacattgca
     5161 agtgccgaag aattggaaga aatccaagcg caagtaaagg aagcagtaga agcttctgtt
     5221 aaatttgcag aggaaagccc attcccaccg cttgaatcag cctttgaaga tatttacgca
     5281 gactaaggag aaagagataa aatggaaaca aaaacaatgt ccttccgtga caccattatc
     5341 cttgctatgt ctgaggaaat gcgtcgcgat gaaaatgtgt tcttgatggg agaagatgtc
     5401 ggtgtcttcg gaggagactt cggtacttct gttggaatgc ttgaagaatt tggtccagaa
     5461 cgtgtccgtg actgtccgat ttctgaagct gccatctctg gagcagcagc aggagcagcc
     5521 atgacaggac ttcgtccaat cgtcgatatg accttcatgg acttctctgt tattgccatg
     5581 gacaatatcg tcaaccaagc tgctaaaaca cgttacatgt ttggtggtaa aggtcaggtt
     5641 ccaatgactg ttcgttgtgc agctggtaac ggagttggct ctgccgccca gcactcacaa
     5701 tctctagagt cttggtttac tcacattcca ggacttaagg ttgtggcacc tggaacacct
     5761 gcggacatga aaggcctgct taagtcttct atccgtgata acaacccagt tattatactt
     5821 gagtacaagt cagaatttaa ccaaaaaggg gaagtgccag ttgatccaga ctacacaatt
     5881 ccacttgggg ttggtgaaat caaacgccaa ggaacggatg taacagttgt tacttatgga
     5941 aaaatgcttc gccgtgtggt tcaagctgct gaagaattag cagaagaaga aatttcagtt
     6001 gaaattgttg acccacgtac ccttgttccg cttgataagg atatcatcat aaactcagtg
     6061 aagaagactg gtaaggttat tctggtcaac gatgcccaca aaacaagtgg ctatatcggt
     6121 gaaatttcag ctattatttc agaatcagaa gcatttgact atctagatgc accaatacgc
     6181 cgttgttcag gagaagatgt gccgatgcct tatgcacaaa acctagaaaa tgcaatgatt
     6241 ccaacagttg aaagtatcaa agatgcaatc cgaaaaactt ataacaaaga ctagagttac
     6301 ataatataag ttatgtaact aaaataaagc tgacgagaaa ctttgtatct tttaggtgca
     6361 gagctcttcg tcattaatat cttatattcg agcacgggct aaaagctcgg aaaaaagata
     6421 aatctccttg gcttcatcga agccatcgtc gatttcctat ttttcagtcg cttttgacgc
     6481 cctttgtatc ataaatagaa ttggagaggt catggctgat gacaagctaa gagcgactcc
     6541 tgcagctaga aagttagcgg atgatttagg gataaacctc tacgacgttt ctggctcagg
     6601 tgcaaacggt cgtgtccaca aagaagacgt ggaaacttat aaagacacaa acgtggttcg
     6661 catttcgcca cttgcaaaac gaattgccct cgaacataac attgcttggc aggaaatcca
     6721 aggaaccggt catcgtggta aaatcatgaa gaaggatgtt ttggccctgc ttcctgaaaa
     6781 tatcgaaaac gatagcatca aatctcctgc tcagattgaa aaagtggaag aagtccctga
     6841 taacgtaaca ccatatggtg aaatcgagcg tattccaatg acaccaatgc gtaaggttat
     6901 tgcccaacgc atggttgaat cttacttgac tgcgccaacc ttcaccctca actatgaagt
     6961 tgatatgact gaaatgttgg ctcttcgtaa gaaggttctt gagccaatca tggaagcaac
     7021 tgggaagaag actactgtaa cagaccttct ttcgcttgca gttgttaaga ctcttatgaa
     7081 acacccatac atcaacgctt cattgacaga agatggcaag actattatca ctcacaacta
     7141 tgtcaatctt gccatggcag ttgggatgga taatggattg atgacacctg ttgtttacaa
     7201 tgctgagaag atgagtcttt cagaactggt ggtagccttt aaggatgtta ttggccgtac
     7261 cttggatggt aaattggctc caagtgagtt acaaaattca acattcacaa tcagtaattt
     7321 gggaatgttt ggtgttcagt cctttggtcc gattattaac caacccaact cagctatcct
     7381 tggtgtcagt tcgacaatcg agaagccagt tgtcgtcaat ggtgaaattg tgattcgccc
     7441 aatcatgagt ttaggattaa ccattgacca ccgtgtcgta gatggtatgg ctggtgctaa
     7501 gtttatgaag gacttgaaag agttgattga aacgccaatc tcaatgttaa tttaagagca
     7561 agagtattca tttttaagat atagataaag gaaggaagac atggccttag aaataattat
     7621 gccaaaagcc ggcgtggata tgacagaagg acaaatcgtc caatggaata aaaaagtcgg
     7681 agaatttgta aaagaaggag aaatcctttt ggaaatcatg actgataaag tcagcatgga
     7741 attggaagcc gaagaagatg ggtacttgat tgccattctc aaaggagatg gtgaaactgt
     7801 ccctgtaacg gaagttatcg gttaccttgg tgaagaaagg gaaaacatcc caacagctgg
     7861 agcagcatcg ccagaagcta gccctgtacc tgtagcaagt acctcaaacg atgatggtaa
     7921 gagcgatgat gcttttgata tcgttgtgat tggtggaggt cctgctggtt atgttgcagc
     7981 cattaaagct gcccaactcg gcggtaaggt tgcccttgtt gagaaatctg aacttggtgg
     8041 aacctgctta aaccgtggat gtattccaac caagacctac cttcatagcg ctgaaattat
     8101 tgaaaatatc ggtcatgctg caaatcgtgg tatcgtgatt gaaaatccta acttcactgt
     8161 tgatatggaa aaacttttag aaactaaatc taaagttgtt aatactcttg ttggtggtgt
     8221 tgcaggactt cttcgtagct acggagttgc tgttcataaa ggaattggta caatcactaa
     8281 agacaagaac gtcttggtaa atggttctga attgcttgaa accaagaaaa tcattcttgc
     8341 tggtggttca aaagtcagca agatcaacgt ccctggtatg gaatctccac ttgtcatgac
     8401 tagtgatgac attcttgaaa tgaacgaagt gccagaaagc cttgttatca tcggtggtgg
     8461 agttgtcggt atcgaactcg gtcaggcctt catgacattt ggttcaaaag tgactgttat
     8521 cgaaatgatg gaccgtatag ttccagctat ggatgtggaa gtttctaaga accttcgctt
     8581 gatccttgag cgtaaaggaa tgaccatctt gactggtact aaactgcaag aaatcattga
     8641 ggaaaatggt caacttcgta tcaaggttga aggaaaagac gatatcatcg caagcaaagc
     8701 tcttctttca attggtcgta tgccagacct tgaaggtatt ggagaggttg agtttgaatt
     8761 ggatcgtggt tgtatcaagg tcaacgaata catggaaact tcagttccag gcatttatgc
     8821 accaggtgac atcaacggta ctaagatgtt ggctcacgca gctttccgca tgggtgaagt
     8881 ttccgctgaa aatgccctta aaggaaatca tgcagttgcc aaattgaatt tgactcctgc
     8941 agccatctac actcttcctg aagtagcagc agtaggtttg acagaagaac aagcccgtga
     9001 gaaatacgat gttgccatcg gtaagtttaa ctttgctgct aacggtcgtg ctattgcatc
     9061 tgacgcagct caaggtttcg taaaagttat cgctgataag aaatacggag aaatccttgg
     9121 tgtacacatt attggtcctg cagccgcaga attaataaac gaagcatcaa gcatcatcga
     9181 aatggaaatc actgttgagg aaatgctgaa gaccatccac gggcacccaa cttactctga
     9241 agtgatgtac gaagcgtttg cagatgttct aggaatggcc atccattcac ctaagaaaaa
     9301 ataaaacgaa gatagattag actggaaggt tatttccagt ttctatcata taaagtaaag
     9361 ggatatcatg aaatacatta tcaatcattc aaatgacact gcttttaata ttgccttgga
     9421 agaatacgcc tttaaacacc ttttggatga ggatcaaatc ttcctacttt ggattaacaa
     9481 gccatctatc attgttggtc gtcaccagaa cactattgaa gaaatcaacc gtgattatgt
     9541 tcgcgaaaat ggtattgagg tagttcgccg tattagtggt ggtggagctg tttaccacga
     9601 tttaaataac ctcaactaca cgatcatctc caaagaagat gaaaataagg catttgactt
     9661 caagagcttc tcaactccgg ttatcaatac cttggctcaa ctcggggtta aagctgagtt
     9721 cacaggtcgt aatgaccttg agattgatgg taagaagttc tgtggcaatg cccaagccta
     9781 tatcaacggc cgtatcatgc accacggttg cttgctcttt gacgttgatt tgtcagtcct
     9841 cgcaaatgcc cttaaggttt caaaagataa atttgaatca aaaggtgtga aatccgtccg
     9901 tgcccgtgta accaatatta tcaatgaatt accaaaaaaa atcacagtcg aaaaattccg
     9961 tgatttactc ttggaataca tgaaaaaaga gtacccagag atgactgaat acgtcttttc
    10021 agaagaagaa ttggccgaaa tcaatcgcat caaggatact aagtttggaa cttgggactg
    10081 gaactatggt aaatcacctg aatttaacgt ccgtcgtgga accaaattca ctagtggtaa
    10141 ggttgaagtc tttgctaacg ttactgaatc aaaaatccaa gacatcaaga tttatggtga
    10201 cttctttggt attgaagacg ttgctgcagt agaagctgtc cttcgtgggg tgaaatacga
    10261 acgcgaagat gttcttaagg cgctaaaaac cattgatatc acacgctact tcgctggtat
    10321 tagccgtgaa gaaatcgctg aagcggtagt tggataaatc aaaaagaagt tggccaacta
    10381 accaacttct ttttttgata tttatttaca taatttaaaa tacgtaaaat cataaactat
    10441 ccagagcatt cttttgttca tcactaacaa tatgggtata gaggtcagtg acttgtgtgc
    10501 tagcatgtcc tagttggtga ctgactaaaa cttgtgattt agtcgcatca tagagcctag
    10561 ttgctagtgt atggcgcagt ttatggggtg ttacacgcac tttaaaatcc tctgagtatt
    10621 tagcaaccat tttctcaacg ctagaagcat cgatacgatt aggaacacct ctgtagagag
    10681 ttaaaaaaag ggctgtatct gttttttccg ttttatagcg ttgattccga atggccagat
    10741 aattctctaa ataaggttta gcaaaagcag cgacattgac tgagtcacgt ttgccacctt
    10801 ttcgagtaac atcaataacc atcattttta gattgagatc tcttagatct agattaacag
    10861 cttcagataa gcgaacacca gatgccaaga gaagggcaat aatggctaaa tctcgttctt
    10921 tatttttgtt gaatgatgag agagctcgat ttgaaagctg ttgtgggtgc tcttgatcga
    10981 tataagttag aaaaccttct gtttcatcac ctagaaagag tttttgcttg atattttcag
    11041 ctctggcagc aagggtttct ttctttttct tggttgaaac ttttttcatt acattacgat
    11101 agaaataagg ttccccctga tcgttttcaa cctcctcggt tagatacttg taaagactag
    11161 aaagtgctga taaggttcga ttgatagttg tctgtgaaac accttgtttt gttgtattag
    11221 cattcagcaa gggacgttca cgtagataaa ggataaagga ttccatgtct ttcttagaca
    11281 tattttccaa aactgataaa ggaatatcgg atattttatc agcgtttgaa ataccagact
    11341 ccaaaaccca gctgaaaaat cggtcatatt ccttaaggta ttcgtacagg gttgtaaaac
    11401 tgtagggcac agccagttta gattggtagt attccagaac ataccagggc atgagttgtt
    11461 ttagtttgtc gattcgttcc agtaaaatct cacgtttcat gtattttctc cataattaag
    11521 tatactagta gtatagcata gacttgtaca tttttcaaga aaattatagt ttttcagaaa
    11581 gatgttatag gagtttttca aagtgataaa aagacttaga cctacaaaca aaacagaata
    11641 aggaaaataa taaaaatctc acccactaaa cttctgttaa tactacatag ctagaaattg
    11701 gtttgattgt cctgattgtt ttatcctatt tctatttcat ttcactatat aattgctatt
    11761 gttttctttt tcatgatttt aatcttactg tacagatttg atacagtaaa ctcctttcta
    11821 gctttatact ttatcatttc taacttaatt atagtcttat ttttaataaa gttcaattat
    11881 ttatatgtaa aattcctcat cagtaaaaaa taaccgatct catttctgag aatcggtttt
    11941 ctaaataaat caagccgaat aaatcaagcc gatcatttac ataacataaa ttatgtaaat
    12001 gatcgtacta caacaacaaa tctttgactt ttccaatttc actttttgga ataaccaggt
    12061 gaatcatatc acccagatac attctggttg agccgttaac tgtttggctc ttgccattat
    12121 ggacttgagt tgtgatgagg acgttgtgtg gtaagttgag ttcatgaact tgtttcccag
    12181 caattttatc agaaactggt atttcgataa gtgtaacttc tccttcgcta gatacttctt
    12241 ctggaagcat tttttccagc atggcttcat agactggcgt acctttgagc aaatccatga
    12301 taatataaga aacaagagtg acaagaccaa gtggcatgag gttgcgaata tctcctacca
    12361 tctcagttac gaggatcata gcagttaagg gagcttttga tatggctcca aaatagccac
    12421 tcattcctag aatgacaaat atagggaatt gctcttgact gacaagtcca agattgacac
    12481 agataacacc aactaaggca ccaagcaaag aaccaagagc taaaatgggg aggaaaattc
    12541 ctcctggcag tccacttcca tagctaatca tactccaaat aaagcggatt aaaaagtaag
    12601 ctaataaaac ttggaaacta aaattttgtt cagttaaaga aaggacaagc tgatttccgc
    12661 caccaatgat ttgaggtaag aagattccga ctggtatgat aaggataaaa gccaagatgg
    12721 gataataagc cctatccaaa tggatttttt gaccaatcaa gtcataaact cttccaacgt
    12781 ttaatacagc tttctcatag agaaaacctg aaaatccaag gaaaattccc atgacgagat
    12841 atatccaata ctgatctagg gtcatgggag gaatgttatc tggcatatcc aatactggtg
    12901 tcaaaccgaa catgagtaga gacacaaagt ttgctacgat gctggctgct agagttgaga
    12961 cccagaaaaa gcgcgaaaaa tggtgataga cttcttctac aacaaagaga agtgctgcaa
    13021 taggagcatt aaaggctgcg gctaaacctg ctgcagctcc actggcaatc aaggaacgtt
    13081 cctctactgg actggatttg agccacttgg caattccttt accaccaact gctccaagtt
    13141 gaatgctggg tccctctcga cccagcatga gtccactggc aatagcaaga atacctagca
    13201 catatttttt ccaaagaatg ccccaccagt tgagggacat gaggcctttc agttcggctt
    13261 cgacttgagg aattcctgag cctttaatat ctttttctga ccgtgttagt ttggcactga
    13321 gccaacagat gagtatataa aacaaaacca gtacaaaaag attgcgcact aagtaccctt
    13381 gatcttgata aactccttgt atcaggtgga agcccttttc aattaagaaa cgaaaggatc
    13441 cgacgataat tccaacgatg aggccgacaa tgattcctcg accaacttgg gatagtatag
    13501 tgctggatgc aaaggcgaat tctttcttag aacggactat ttccgactgt tcctccatgg
    13561 tatttccttt caattaattt tcttttttac ctaaaaccag tgatttaacg ggttcaaagc
    13621 tggttcggtg aattggggta actcctagtt ttgtgaggcc ttccagatgt ttagctgtgc
    13681 catatcctgc attagtagcg aaatcatagc cagggaactg ctgatcgtat tccttcatca
    13741 attcatcacg tgttaccttg gctactatag atgctgctgc gatagagagg gagttggcat
    13801 ctcctttgat aatggaggtt tgtgaaatgg gcaagtccag tttcatggca tcaatcaaaa
    13861 ggtgctctgg ttgagggctg agctgggaga ttgcttcttg catggctagt ttggttgctt
    13921 catagatgtt gacttggtcg atgacctgat tatctatgat accaattcca atcgacaagg
    13981 cttggtcttg aacggcttgg aaaatctcca gatgtttctt tttaggaatt ttcttgctgt
    14041 cgttgagacc tttaatctta caatttttag gtaaaatgac ggccgcagcg actacaggac
    14101 cagcaagagg accacgacca acctcatcaa tacctacaat taaggtcaat ccttgcttat
    14161 aaagttcttt ttcataagaa agcatggatt ccaagcgcaa attttcatct aattcagctt
    14221 gaatggctct tttacgcttg ctgatttcct tttgaactcc tgagcgatta tccttttcaa
    14281 gctctaaaaa aatagggctt tctaactcct tgactgtcac aaggagttct ttgatttctt
    14341 taatcgttgc catcgaggtc ttccaatgta tctaaggtat agttaccgag tttgccatca
    14401 cggacttcct tcacgaagag actgtaaaaa cggtcatagt catcacggaa accgagggcg
    14461 cgggtcatat ccataataat cacaggcgct tcttcttcaa ttttcatttg tttgaagcgt
    14521 tcagccagct tttctggata atgttctttg aaataattga taccaaaaat ggtaacctca
    14581 tccataggaa gcaactgatc cttgatagct ccagtcaatg ccaacttaag tgcaacagtt
    14641 tcatcctcaa acttaggcca gagaatcccc ggtgtatcca agatttccag atctttattg
    14701 gttttaagcc attgttgacc ttttgtgacc cctggcttgt ttccaacaac agcaatcttt
    14761 ttaccagcca aacggttcat cagagtggat ttaccagcgt ttggaatccc gataatcatg
    14821 gtacgcaagg tttcaatctg aatcccacgt tctttctggc gagcaatctt atcagccatg
    14881 agcttcttgg ccgcatctgt tacaactttt acagtcactt gctctttgga gttgatagct
    14941 agcgtctgga tttcttgtga ttcaaaatac tgacaccatt ccttggtcat tgctggatca
    15001 gccaagtcgg ccttgtttaa aatcaagagt tttggtttat caccaacaat cttggtcaac
    15061 ataggatttt gactagatag aggcaagcgt gcatctacta aaatcgtcac aaaatcaaca
    15121 aattttaaat tttcctgcac ctgtcgacgc gctttagaca tgtgaccagg aaaccattga
    15181 atagtagcca ttgagttttt tcctttcgta gctctgaagc tgttaatcta gaactaagag
    15241 atctcaatct aaaaatgaat gatgtccaaa cagttcttgt tttatgatat aaaaatctac
    15301 tttatattat atcatgcctt ctttattttg tccgatgata gccttttacc ctatctttta
    15361 ccctatatag aaaaaagcca tcaaatgatg acttttcaga gggagcaaaa aactgtcctc
    15421 tttatatttt aaacatttaa atgtcttctt taaacagttt attaaagaaa ccgtctgtgt
    15481 ttttaagaag ggctttgaat actttggatt tcaagtctac tgtatttttg aaatgatctt
    15541 taattttcgg atctgacata ccatcaatat ctttttgaac agcttcagca aataaagttt
    15601 tcaactgagc ataatttgta atttgacgac catcaatttc aatagcagta aatccttttt
    15661 ctgcttttga cttaacatct ttgaagtatt gtttcttcca ctcttcaaga ttattgaatt
    15721 tatccttaga tactttcttg ataatgaaat catcacccaa taatttttta ttggttttgt
    15781 cagcttcgcc ttgatatttg ctagatgcat aactaatgaa tccattttca taaccaaaat
    15841 agccccacat acggaaagta ttatgcttga atgatgcagc tccaggtgct ccttggctag
    15901 tattaccacc atagataccg gtcattatat taatatttac gtaagcacta tcaaaatctt
    15961 ctggattata tgttctgtca ctaggagcgc catgttttgt catcaaatta ttgttgacta
    16021 aatcatcaat gttgttgata acaattttct tcttctcatc ttcagttaat tcacgaactt
    16081 tatcaaattg atgtagctct ccatttttgc gatattgtct atccattttc ttgaaccatc
    16141 gactaatatt accatgtaat ttagggataa cagcatcagc ctcaagatgg tctaacatca
    16201 taagagcatc attataattt ctcatataac ggtcgatatc ttcacgagtt ttcaatttat
    16261 ctggatcata attataccat tggtcaccgt cattttcacg atgataagcc atgttaagac
    16321 caagagcacc atattcaccg ttaccagatt tatcaggtgt ctgcaacata ccttgcgcat
    16381 aggcttcaac gtctgtacct tgacggtgtc tatgcccacc gaagtaaagc attctatcgt
    16441 ttacgtgtgt tgtttcgtga gtaaaggctg aaataccaaa cggactaatc atatttgttc
    16501 ccatgaaata aacttggtca ttattattgg gatcttggaa aatagatgcc atagctccca
    16561 tgtaccaatt tgtagtatga taacgccaag taggtccata taattcacga ataggtgcaa
    16621 ttggtttatt attcttatca taacccatac gatttacacc ttcgacgtta tgatagtttt
    16681 ggttatccca aacaggtgtt ggaaccctat tttgactctt gagtaattgg tcacgtacgc
    16741 ttggcaatgc taaacgcgac cagaagtcaa gataagtttg ttgtgcgtga gcaacttctt
    16801 taattttatc tttaaatgct tctcgttcag cttctgtata cttaccatat ttttcaaaag
    16861 agctataagc cattgtattg taagtcgaaa tcaagaacat atgtgcattc ttaagagtta
    16921 atagaggtag gatcatacga ttatgaacac cattattcaa accatcaaat gcacgatgtc
    16981 gtttattaac aaaatctggg ttcgttgttt taggctccac aatgtatacg ttcttagccg
    17041 catggataaa ccaatcattt aaatcagtat cgttagtgaa gagacgcatg ttgtaatcta
    17101 agaagccatg taattcagac ttaccaatag ttccaccaat catatcacga taagcatcca
    17161 aagttctgtc acccttgata tttcgctcac ttgatccaat tcgaatcaag aagtcaagaa
    17221 ggtcaactga cttaccatag aaatcaggtt taaacatcat caagtttttg atagtcatat
    17281 ccccatactt tatatcatag taacgattaa ggtaagtaag agccatcacg attttagctt
    17341 tattttcatc caccttctta acaagtgctt tcatagctgc ttcgtcacta tttaattgat
    17401 ggtcagcatt ttcaatcaaa gatttgacca atttatcaag attagctttc gtttcagaaa
    17461 aactttcttc gaaataaagg tctcggatat aaccattctt acgctcatcc gaattagcat
    17521 taggatctct agatgtatct cgtttatcca tcaaagcacg gacttcggga gaaattaatt
    17581 caacagttgc taacctatct ttaatgtctt taatcaattg atcacgattt ttatcaacca
    17641 tgtttggtgt ataaacaatg tcatctaatc catcgatact atactcacga acttgcgcta
    17701 ctttagactc tttttgatga acagctttga tttctttagt cttatctgca tagtgaatca
    17761 tcacatgatc aatatctgat aaatcggtca cgaattgacc atccttcata cccgtaacag
    17821 aaaggacttc tttcttagct aagtttgaat cttctgcaag tttgttacct tgattgacaa
    17881 tccattcttt gttataaaat ggttgcagtt tctcaatatt acgataagcc agatctcgat
    17941 ctactttata gtcttgggtt gacttatatt catcttctct tctggttaaa cgatttaagc
    18001 gattgaccac aggttcattg atttcgtatt tatctgcggt aattcctgta gcttcaattt
    18061 tagattttgc ctcttcctca gaaataggtc tgatacgatt tttatctttt gaacgctggt
    18121 agctagaatg cccatcacta acacctatta caacataatt tcgtcttaca ttatctcctg
    18181 tccagtagcc atcatcataa tcaatatcac tagaaccata gaaaatttca ccattaggta
    18241 ccttcatcat ggaaactgaa tcttcgacag aaccgtggtg cttcatgctt cctacaacac
    18301 caccactttc aatcgcctca gttacttgaa ttgttccctt agcaatagat tttttgattg
    18361 ctccttcctg acgaacagta ttgttatttc cttcattttg tgtccaataa gcaatgcccg
    18421 cagccttggc tttcttgcca ataagatttg cgttaacata cgcgcgttcg acgagtcctt
    18481 tccagttctc accaacgata ccagtcaaga attgtccacc atcgccgaca gaagttacgt
    18541 taccgataaa ggccacattt ctgagtgtac cttggttatc aatcttatca ataaatccag
    18601 atacccaatc tttacctact atattacctt taaccttaac attttcaatg gtagttccac
    18661 ctttaataat attcgctaca gctgcaactt ggttcgccca tggcatattt atgtcaacat
    18721 taccaagatt aatattctta atcgttccac caataacatt gccaaataac ggacgtgaca
    18781 agttatgaat ggtatagtga ttatcaccat cactcaagag cttacctttg aaatctttcg
    18841 taacatatga tttaccaaat gctgatacat ttgtggcatt aaggtcagca cccaatttaa
    18901 actcgtcatt tggatgttct tgcatatctc tcacaagttc attgaaattg taatatacat
    18961 tattgacttt tggaactggt ttttcaatgt aatgaatgta ttcatttcta gtcttagatg
    19021 gatcgacatg ttgaattaaa tcttgagcgc ttgctgtaat tttataaagt tctttttctt
    19081 tttcagttac taactcaatc ttatcaactg ctaaacgagt tactttatta tcacgactag
    19141 tgatttttaa gtacattttc ttcacatctt catcagaagg tttttcgctc atgaaatcac
    19201 taggtatttc agtaccatca tcaccatcat catttacctt cactaaatta gtagatgaaa
    19261 tatttttaat ttcgaccttt ttcaactcca aacgcaaagt tttttcatcc agtttagacg
    19321 tttcagaacc ttgaccacga tcataaatca ttgttgttgc aatcttataa tctttaaaca
    19381 aatccaaatt ttgtacagcg tcagttaatt tttctttaga taaatcaacc tttttaacta
    19441 ctgtatcacc atctttaatt tcagctataa tacttgatat ttcagcatta tctttattat
    19501 caagctcata atttaagttg gcagaacgat tcatagcatc ttctgttact ttggtcaagg
    19561 tcaaaacggg aactgttttt tcttttggat tttcttttgt accttttaca agaattttag
    19621 gtttcatttc tttcaaaatt gttaccgttt caactggttc actaattttt ttgccatgta
    19681 actcttcata gctagtaaca atttgacgtt cgccatcctc accaacttct ttaacagaat
    19741 catcgcctat aaatttgcta ggatcagatt cttctatagt ggttttagga atagtctctg
    19801 tacgaatatc ggtatgtaaa gaaggcaact cttctctttc tgatgcaact tctgaaactg
    19861 tagatattgg ttcagtatac tctggaatgt cgttaacagg tggttcaatc aagtttccat
    19921 tttcatccac tcctgttgta cctactggtt cagtgtatgc tagtttctca tgaacttcag
    19981 gctcaacaag gtttgcacct attggttgcg tatagtcagg cttctcatga acttctggtt
    20041 cgcctttgtc agtcacaaca gcttctggta atgtcggttg tacttccggt tcgcctttgt
    20101 cagtcacaac agcttcgggt aaggctggtt gaacctccgg ttcccctttc tctgttacaa
    20161 ctttgggttg ctcaactggc gaaacagttg atgctggaga aaccgacgaa acagttttat
    20221 catcaagctc caagtaacca acatatctgt aaccagaaat ttgcaacaca ccatcacgtc
    20281 cagactcatg gatgttagca ttcaaattaa gtgctgaagc agttgatagt gtaactaaac
    20341 ctgtcgcccc tacaatcaaa aatgtagcta tttttttatt tctattatct tttgtgataa
    20401 tcacaataag actcccgata gctaacaaac ccaatgcagt cataatagat tgagaactgc
    20461 ctgtgtttgg tagtgcgtct ttttcataat ccaaagcata tgactctttg gattcatcag
    20521 gtctaccttg tttgagttga tcacgttcag tttgtgtcaa actactataa tctaagtaat
    20581 gataagtcga tgcaccaata actgacggtg caaataaaag actccccaag aataccgaac
    20641 caacaatacc ctttattttc cgaatggaaa atttatcttt ttttaataat gacaattcta
    20701 atcctccaca ttcacttttt atttcctaat agtaaacact attttttatt ctcaaaaatt
    20761 gaacttctaa aatcatttgt ttggtcaaga taagctttat atattgcttt tttcagttta
    20821 agaactctac tatttgtttc tgggtaaaat tttgctacgt catcttgcgc atcatctctc
    20881 actgcttcga caatcattct ctctagtaca cctatatccg taaccgatac atttcgttga
    20941 cgactcaatg gattattagg attaataaag ttaactctat ttagtttact gaattgtttt
    21001 tctctttcct tatacatatc cttcttgaat tgtgtccaag ttttatattg accattaaat
    21061 actttttcaa gtactaattc atctgtaact aaacctactt ctttaccata agaattaatc
    21121 ttacttcccg ccgcttttgc atctttttca tattgatttg agatataagg taccatgccc
    21181 tctttatatc cttttgcagc taataattcg aatgctatac ggcgtcccat taaatcacca
    21241 ggagtccctt tctctccact aagagctgaa taaatcggtg aaaataaatc aattgtataa
    21301 taaccatttc tcttatattc ttggtctttg taccctctgc tagtaataat atcattttca
    21361 attaaactat caaaagaatt taatttatta acttcatcca tcgttaatct gcgaacaaca
    21421 tttgtcgcgt atacattatt accgtcttga tcaagatcaa atttattttc aatttttctt
    21481 agtaactcat atttttgatt tgtttctaaa ttagctacag cttttccttc taaatattct
    21541 aacatataaa ttacatcaaa catattatgc atataattat gtaggtcttc tgcactatta
    21601 aatcgttgag ttggatcagc tacttgaagt cttgttgaat tttctgaatc ctcatatttc
    21661 aagacagagt taattgttat tgttggatca tatggatgat ccggagcttg taataatcct
    21721 tttgcataaa actcaggtcc taatccgctt cttcttccat atcctccaag ataaatctct
    21781 ctatctgagt tatgtgtcat ttcatgagta tatgttacag ctccatcttt atccaacata
    21841 cgatatgcca tataatagaa tgcatctccg gtagcatatg ctccgtgtcc attatgaaca
    21901 acattatttc ccgcaggtcc aaagaagtat tttatagctg gatgatcagt ttcaaaagta
    21961 gcttgatgag ttactttgtc tttatctttg tcagtaccaa atttgaaagc atcatatact
    22021 agaatacttc tatataattt ttctttatta tcgttatcta atatcttata ccaataatca
    22081 taatgatctc taaatcgttt agccgcttct ttggcgttat cttctacaaa tttgtttaac
    22141 ttttctccct taggatattc tgaacttcta tatctgtcat aggctccaaa tccaatagta
    22201 gaaatattcg ctatcataaa cactgttttt tcttcaggta aagttaatag tggtaagacc
    22261 atactttgat atttccaaga tggattagtt aatctatcat ataccccaat agaatattta
    22321 gttccctctt ttccttgttt ttctcttccc tcttcaattg tagatttttc ttctacaata
    22381 tatgcttttg tttgtttttt aaaccactca ttgttagaaa ttgttggtaa gaatactttt
    22441 ctatatcctt ctaatgcttt aaataaactt tcagttccat aatttttcgc taataataca
    22501 ttataagtaa ttacattatt ttttgctaat aaattgttaa atccagattt a
//
LOCUS       DAVKQB010000036        22116 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKQB010000036 DAVKQB010000000
VERSION     DAVKQB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.036"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..267
                     /locus_tag="VW528_001428"
     CDS             1..267
                     /locus_tag="VW528_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
                     /protein_id="HEU9912239.1"
                     /translation="MLVVLLIISVLFLLFVPNLTKQKEAVNDKGKAAVVKVVESQAEL
                     YSLEKNEDASLSKLKDDGRITEEQVKAYKEYNDKNGGANRKVND"
     gene            236..664
                     /locus_tag="VW528_001429"
     CDS             236..664
                     /locus_tag="VW528_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9912240.1"
                     /translation="MEEQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLPVPKGIQAPSGQSIIF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            669..929
                     /locus_tag="VW528_001430"
     CDS             669..929
                     /locus_tag="VW528_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912241.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQININGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            892..1353
                     /locus_tag="VW528_001431"
     CDS             892..1353
                     /locus_tag="VW528_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9912242.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVRFRFQFQKGLEREFIYRVEKEKS"
     gene            1331..1744
                     /locus_tag="VW528_001432"
     CDS             1331..1744
                     /locus_tag="VW528_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9912243.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            1881..2468
                     /locus_tag="VW528_001433"
     CDS             1881..2468
                     /locus_tag="VW528_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9912244.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKEILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQAHLKAEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            2529..3482
                     /locus_tag="VW528_001434"
     CDS             2529..3482
                     /locus_tag="VW528_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9912245.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVELFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            3533..4723
                     /locus_tag="VW528_001435"
     CDS             3533..4723
                     /locus_tag="VW528_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9912246.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            4872..5243
                     /gene="rnpA"
                     /locus_tag="VW528_001436"
     CDS             4872..5243
                     /gene="rnpA"
                     /locus_tag="VW528_001436"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9912247.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            5218..6042
                     /locus_tag="VW528_001437"
     CDS             5218..6042
                     /locus_tag="VW528_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9912248.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            6061..7047
                     /locus_tag="VW528_001438"
     CDS             6061..7047
                     /locus_tag="VW528_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9912249.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            7098..7721
                     /locus_tag="VW528_001439"
     CDS             7098..7721
                     /locus_tag="VW528_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9912250.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLEKQAKILDIPTVKGIYYLAYSDGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            7852..9309
                     /locus_tag="VW528_001440"
     CDS             7852..9309
                     /locus_tag="VW528_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9912251.1"
                     /translation="MEVISSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFIFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            9332..9613
                     /locus_tag="VW528_001441"
     CDS             9332..9613
                     /locus_tag="VW528_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9912252.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            9690..10175
                     /locus_tag="VW528_001442"
     CDS             9690..10175
                     /locus_tag="VW528_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9912253.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            10192..10857
                     /locus_tag="VW528_001443"
     CDS             10192..10857
                     /locus_tag="VW528_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9912254.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            10861..11724
                     /locus_tag="VW528_001444"
     CDS             10861..11724
                     /locus_tag="VW528_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9912255.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            11726..12430
                     /locus_tag="VW528_001445"
     CDS             11726..12430
                     /locus_tag="VW528_001445"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9912256.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIIVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            12609..14282
                     /locus_tag="VW528_001446"
     CDS             12609..14282
                     /locus_tag="VW528_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9912257.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            14394..15485
                     /gene="ulaG"
                     /locus_tag="VW528_001447"
     CDS             14394..15485
                     /gene="ulaG"
                     /locus_tag="VW528_001447"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9912258.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            15599..17575
                     /gene="tkt"
                     /locus_tag="VW528_001448"
     CDS             15599..17575
                     /gene="tkt"
                     /locus_tag="VW528_001448"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9912259.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            17692..17991
                     /gene="yajC"
                     /locus_tag="VW528_001449"
     CDS             17692..17991
                     /gene="yajC"
                     /locus_tag="VW528_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9912260.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            18038..18466
                     /locus_tag="VW528_001450"
     CDS             18038..18466
                     /locus_tag="VW528_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9912261.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQQYEILYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            18468..18878
                     /locus_tag="VW528_001451"
     CDS             18468..18878
                     /locus_tag="VW528_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912262.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            19179..21830
                     /gene="adhE"
                     /locus_tag="VW528_001452"
                     /gene_synonym="adhC"
     CDS             19179..21830
                     /gene="adhE"
                     /locus_tag="VW528_001452"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9912263.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         6964 a         3967 c         4827 g         6358 t
ORIGIN      
        1 atgttggtgg tcttgctgat tatcagcgtg cttttcttgc tctttgtacc taatctgacc
       61 aagcaaaaag aagcagtcaa tgacaaagga aaagcagctg ttgttaaggt ggtggaaagc
      121 caggcagaac tttatagctt ggaaaagaat gaagatgcta gcctaagcaa gttaaaagac
      181 gatgggcgaa tcacggaaga acaggttaaa gcttataaag aatacaatga taaaaatgga
      241 ggagcaaatc gtaaagtcaa tgattaaggc ctttaccatg ctggaaagtc tcttggtttt
      301 gggtcttgtg agtatccttg ccttgggctt gtccggctct gttcagtcca cttttgcggc
      361 agtagaggaa cagattttct ttatggaatt tgaagaactc tatcgggaaa cccaaaaacg
      421 cagtgtagcc agtcagcaaa agactagtct gaacttagat gggcagatga ttagcaatgg
      481 cagtcaaaag ttgccagtcc ctaaaggaat tcaggcccca tcaggccaaa gtattatatt
      541 tgaccgtgct gggggcaatt cgtccctggc taaggttgaa tttcagacca gtaaaggagc
      601 gattcgctat caattatatc taggaaatgg aaaaattaaa cgcattaagg aaacaaaaaa
      661 ttagggcagt gattttactg gaagcagtag tcgctctagc tatctttgcc agcattgcga
      721 ccctcctttt gggacaaatt caaaaaaata ggcaagagga agcaaaaatc ttgcaaaagg
      781 aagaagtctt gagggtagct aagatggcct tgcagacggg gcaaaatcag ataaacatca
      841 acggagttga gattcaggta ttttctagtg aaaaaggatt ggaggtctac catggttcag
      901 aacagttgtt ggcaatcaaa gagccataag gtcaaggctt ttaccttgtt agaatccctg
      961 cttgccctgc ttgtcatcag tgggggctta ctcctttttc aagctatgag tcagctcctc
     1021 atttcagaag ttcgctacca gcaacaaagc gagcaaaagg agtggctctt gtttgtggac
     1081 caacttgagg cagaattaga ccgttcgcag ttcgaaaaag tagaaggcaa tcgcctatac
     1141 atgaagcaag atggcaagga catcgccatc ggtaagtcaa agtcagacga ttttcgtaaa
     1201 accgatgcta gtggacgagg ttatcagcct atggtttatg ggctcaaatc cgcacagatt
     1261 acagaggaca atcaactggt gcgctttcgt ttccagtttc aaaaaggctt agaaagggag
     1321 ttcatctatc gtgtggaaaa agaaaaaagt taaggcaggt gttctcctct acgcagtcac
     1381 catagcagcc atctttagtc ttttgttgca attttatttg aaccgacaaa tcgcccacta
     1441 tcaagactat gctttaaata aagaaaaatt ggttgctttt gccatggcta aacgaaccaa
     1501 ggataaagcc gagcaagaaa gtggggaaca ggtctttaat ctaggtcagg taagctatca
     1561 aaacaagaaa acaagcttgg tgacgactgt tcgtacgtct aagagccaat atgagtttct
     1621 gtttccttca gtcaaaatca aagaagagaa aagagataaa aaggaagagg tagcgaccga
     1681 ttcaagcgaa aaagtggaga agaaaaaatc agaagagaag cctgaaaaga aagagaattc
     1741 ctagtcaatt caactataat gcgttgaatc cagaatagtt cactgtagtt tctagaaaat
     1801 tgctggaaat ggatgttaag ctccaattta tttgtttata tcttatttca gtttactata
     1861 ctttgtgcta aactaaagat atgaaacatg attttaacca caaagcagaa actttcgatt
     1921 cccctaaaaa tatcttcctc gcaaacttgg tatgtcaagc agtcgagaaa cagattgata
     1981 ttttatcaga caaagaaatt ttagatttcg gtggtggcac gggtctatta gccttgcccc
     2041 tagccaagca ggctaagtca gttactcttg tagacatttc tgagaaaatg ttggagcaag
     2101 ctcatttgaa agcggagcag caagcaatca agaatatcca gtttttggag caagatttac
     2161 cgaaaaatcc cttggagaaa gagtttgatt gccttgctgt tagtcgggtt cttcatcata
     2221 tgcctgattt ggatgcggct ctctcactgt ttcatcaaca tttgaaggaa gatgggaaac
     2281 tcatcattgc tgattttacc aagacagaag ctaatcatca tggatttgat ttagctgaac
     2341 tggaaaacaa gctaattgag catggttttt catctgtgca tagtcagatt ctctatagcg
     2401 ctgaagactt gtttcaagga aatcactcag aattcttttt aatagtagcc caaaaatcac
     2461 tcgcctagtc agggagtgat ttttctataa ggatggaaaa aagaagggaa atttgataag
     2521 ataggaatat ggattttgaa aaaattgaac aagcttatac ctatttacta gagaatgtcc
     2581 aagtcatcca aagtgatttg gcgaccaact tttatgacgc cttggtggag caaaacagca
     2641 tctatctgga tggtgaaact gagctaaacc aggtcaaaga caacaatcag gcccttaagc
     2701 gtttagcact acgcaaagaa gaatggctca agacctacca gtttctcttg atgaaggctg
     2761 ggcaaacaga acccttgcag gccaatcacc agtttacacc ggatgctatt gctttgcttt
     2821 tggtgtttat tgtggaagag ttgtttaaag aggaggaaat tactatcctc gaaatgggtt
     2881 ctgggatggg aattctaggc gctactttct tgacctcgct tgataaaaag gtggattact
     2941 tgggaatgga agtggatgat ttactgattg atctggcagc tagcatggca gatgtaattg
     3001 gtttgcaggc tggctttgtc caaggagatg ccgttcgtcc acaaatgctc aaagaaagcg
     3061 atgtggtcat cagtgacttg cctgtcggct attatcctga tgatgccgtt gcgtcgcgtc
     3121 atcaagttgc ttctagccaa gaacatactt acgcccatca cttgctcatg gaacaaggat
     3181 ttaagtacct taagtcagat ggatatgcta tttttctagc tccgagtgat ttgttgacca
     3241 gtcctcaaag tgatttgttg aaagtctggt tgaaagagga ggcaagtctg gttgctatga
     3301 ttagtctgcc tgaaaatctc tttgctaatg ctaaacaatc taagactatt tttatcctac
     3361 agaagaaaag tgaaatagca gtggagcttt ttgtttatcc acttgctagc ttgcaagatg
     3421 caagtgtttt aatgaaattt aaagaaaatt ttcaaaaatg gactcaaggt actgaaatat
     3481 aaaatagatt ttgttataat agttgaaaac gcttaaaaag gggtatcatg ttatgacaaa
     3541 aacaattgca atcaatgcag gaagttcaag cttaaaatgg caattatact taatgccaga
     3601 ggaaaaagta ttggcgaaag gcttgattga acgtatcggt ttgaaagatt caatttcaac
     3661 tgtaaaattt gacggccgtt ctgaacaaca aattttggat atcgaaaatc atacacaagc
     3721 cgttaaaatt ttattggatg acttgattcg tttcgatatt atcaaggctt atgacgagat
     3781 tacaggtgtt ggccaccgtg ttgtagcagg tggagaatat ttcaaagaat caacagttgt
     3841 tgagggagat gttttagaaa aagttgaaga gttgagtttg ttggctcctc tacacaaccc
     3901 ggccaatgca gcaggtgttc gtgccttcaa ggaattgttg ccagacatta ccagtgtagt
     3961 tgtttttgat acttccttcc acacaagtat gccagagaaa gcttatcgct accctctacc
     4021 aacaaaatat tacacagaaa acaaggttcg taaatacggt gctcatggta caagtcacca
     4081 gtttgtagca ggagaagctg caaaactctt gggacgtcca ttagaagact tgaagttaat
     4141 tacctgtcat attggtaacg gaggctcaat tacagctgtg aaagccggca aatctgtaga
     4201 cacttctatg gggttcactc ctcttggtgg tattatgatg ggaacgcgta caggggatat
     4261 tgatccagct atcattcctt atttaatgca atatacagag gattttaaca caccagaaga
     4321 tatcagtcgt gttcttaacc gtgaatcagg tcttttggga gtttctgcta attctagcga
     4381 tatgcgcgat atagaagcag ctgtagcaga agggaatcac gaggctagct tggcttatga
     4441 aatgtatgtt gaccgtatcc aaaaacatat cggtcagtac cttgcagtgc taaatggaac
     4501 agatgccatt gttttcacag caggtgtcgg tgaaaatgca gagaatttcc gtcgtgatgt
     4561 aatctcaggg atttcgtggt ttggttgtga tgttgatgat gaaaagaatg tctttggcgt
     4621 tacaggagac atctcaacag aggcagctaa aatccgtgtc ttggttattc caacagatga
     4681 agaattagtc attgcccgtg acgttgaacg cttgaaaaaa taagtgaaac taaaaaaata
     4741 ttcaatacaa ggagttggga aagttatttt tccagcttct ttttctgatg aaattgtcca
     4801 aaaccttgct atgattggct tttttgaaaa atatggtata atagtagtaa tttaatagat
     4861 ggagttgagt tttgaagaaa aactttcgtg taaaaagaga gaaagatttt aaggcgattt
     4921 tcaaggaggg gacaagtttt gctaatcgca aatttgtggt ctaccaatta gaaaaccaga
     4981 aaaaccattt tcgagtaggt ctatcagtta gcaaaaaact ggggaatgcc gtcactagaa
     5041 atcaaattaa gcgacggatt cggcatatta tccagaatgc aaaagggagt ctggtagaag
     5101 atgtcgactt tgttgtcatt gctcgaaaag gagtcgaaac cttgggatac gcagagatgg
     5161 agaaaaatct actccatgta ttaaaattat caaagattta ccgggaagga aatgggagtg
     5221 aaaaagaaac taaagttgac tagtttgcta ggactgtctc tgttaatcat gacagcctgt
     5281 gcgactaatg gggtaactag cgatattaca gccgaatcgg ctgatttttg gagtaaattg
     5341 gtttacttct ttgcggaaat cattcgcttt ttatcgtttg atattagtat cggagtgggg
     5401 attattctct ttacggtctt gattcgtaca gtcctcttgc cagtctttca ggtgcaaatg
     5461 gtggcttcta ggaaaatgca ggaagctcag ccacgcatta aggcgcttcg agaacaatat
     5521 ccaggtcgag atatggaaag cagaaccaaa ctagagcagg aaatgcgtaa agtatttaaa
     5581 gaaatgggtg tcagacagtc agactctctt tggccgattt tgattcagat gccggttatt
     5641 ttggccctgt tccaagccct atcaagagtt gactttttaa agacaggtca tttcttatgg
     5701 attaaccttg gtagtgtgga tacaaccctt gttcttccga ttttagcagc agtattcacc
     5761 tttttaagta cttggttgtc caacaaagct ttgtctgagc gaaatggcgc tacgactgcg
     5821 atgatgtatg ggattccagt cttgattttt atctttgcag tttatgcgcc aggtggagtc
     5881 gccctatact ggacagtgtc taatgcttat caagtcttgc aaacctattt cttgaataat
     5941 ccattcaaga ttatcgcaga gcgcgaggcc gtagtacagg cacaaaaaga tttggaaaat
     6001 agaaaaagaa aagccaagaa aaaggctcag aaaacgaaat aaataaggag gaatctggta
     6061 atggtagtat ttacaggttc aactgttgaa gaagcaatcc agaaaggatt gaaagaatta
     6121 gatattccaa gaatgaaggc tcatatcaaa gtcatttcta gggagaaaaa aggctttctt
     6181 ggtctatttg gtaaaaaacc agcccaagtg gatattgaag cgattagtga aacgactgtt
     6241 gtcaaagcaa atcaacaggt agtaaaaggc gttccgaaaa aaatcaatga tttgaacgag
     6301 cctgtgaaga cggttagtga agaaaccgtt gaccttggtc atgtagttga tgctattaaa
     6361 aaaatagagg aagaaggtca aggtatttct gatgaagtca aggctgaaat cttaaaacat
     6421 gaaagacatg ccagcactat cttagaagaa actggtcaca ttgagatttt aaatgaactt
     6481 caaatcgagg aagcgatgag ggaagaagca ggcgctgatg accttgaaac tgagcaagac
     6541 caagctgaaa gtcaagaact agaagacttg ggcttgaaag ttgaaacgaa ctttgatatt
     6601 gaacaagtag ctacggaagt aatggcttat gttcaaacga ttattgatga catggatgtt
     6661 gaggctacac tttcaaatga ttataaccgt cgtagcatca atttgcaaat tgatactaac
     6721 gaaccaggtc gtattatcgg ctaccatggt aaagtcttga aggccttgca actgttggct
     6781 caaaattatc tttacaactg ctattcaaga accttctata tcactattaa tgtcaatgat
     6841 tatgttgaac accgtgcaga agtcttgcag acctatgcgc aaaaattggc gattcgtgtt
     6901 ttagaagaag gacgcagtca tcaaacagat ccaatgtcga atagtgaacg caagattatc
     6961 caccgtatta tttcacgtat ggatggcgtg accagttact ctgaaggtga tgagccaaat
     7021 cgctatgtcg ttgtagatac agaataagta aaatcagggt tgacctgatt ttttgctagc
     7081 tagaggaggt taaactgatg ttgaataaga taagagacta tctagacttt tctggtttgc
     7141 agtaccgtag tcctgataaa gcgggagcag agcgagagaa gatgctggca ttccgtcaca
     7201 aaggacaaga ggcccgaaag gcttttacag aactggccaa agcctttcaa gcaagccatc
     7261 cagaatggca actccaacag actagtcagt ggatgaatca ggcccagcgt ttgagaccac
     7321 atttttgggt ttatctacag agagacggac aagtgacaga acctatgatg gccttacgtt
     7381 tgtatgggac atctactgac tttggaattt ctttggaagt cagtttcatc gaacgtaaga
     7441 aggatgagca aactttagag aaacaggcca agattttaga cattccaacc gttaaaggaa
     7501 tttattatct agcctactct gatggccaaa gtcaacggtg ggaggcgaac gaagaaaagc
     7561 gtcgtacttt acgcgagaag gtgagaagtc aagaagttcg aaaagtttta gtgaaggtag
     7621 atgttcctat gacagaaaat tcgtctgaag aagaaatcgt agaaggctta ttgaagtctt
     7681 attctaaaat tcttccctat tatctagcta cgagaaaata agataatttg taaaacatca
     7741 taaatcatac agtccaagag tgaacagtcc gctgtgtaat tcttggtctt tttgtttgcg
     7801 ctttcgcatt atataataaa cttacaaaaa caattcaaaa ggagaacaat tatggaagtc
     7861 atttcaagtg ttctaaattg gttttctagc aatattttgc agaatcccgc atttttcgta
     7921 ggtttattgg tgttgatagg atatgcactt ttgaaaaaac ctgcccatga cgttttttca
     7981 gggtttgtta aagcaacagt agggtatatg ttgcttaacg tgggtgctgg tggtttggtt
     8041 acaacctttc gtccaatctt agcagctctt aactacaaat tccaaattgg tgcagcggtt
     8101 atcgaccctt actttggact tgctgcagca aacaacaaaa ttgcagcaga gtttccagat
     8161 tttgttggaa ctgcaactac agctctattg attggttttg gaataaatat cttgctcgta
     8221 gctcttcgaa agattacgaa ggtaagaacc ctctttatta ctggtcacat catggtacaa
     8281 caagctgcaa cagtatctct tatggttcta ttcttagtac cacaattgcg caatgcttac
     8341 ggtacagcag cgattggtat catctgtgga ctttactggg cagttagttc aaatatgact
     8401 gttgaggcaa ctcaacgctt gactggtggt ggcggatttg cgattggtca ccaacagcaa
     8461 tttgcaatct ggtttgtaga taaagtagca ggacgctttg gtaagaaaga agaaagttta
     8521 gacaatctta aattacctaa gttcctctca atcttccacg atacagttgt tgcatctgct
     8581 accttgatgc tcgtattctt cggagccatt cttttaatct tgggtccaga cattatgtct
     8641 aataaagaag tcatcacttc aggaactcta ttcaatcctg ctaaacaaga tttctttatg
     8701 tacattatcc aaacagcctt taccttctca gtttacttgt tcgttttgat gcaaggtgtc
     8761 cgaatgttcg tatctgagtt gacaaacgcc ttccaaggta tttcaaacaa attgttgcca
     8821 ggttcattcc cagcggttga cgttgcagct tcttatggat ttggttctcc aaatgctgtc
     8881 ctgtcaggat ttatctttgg tttgattggt caattgatta caattgtttt gctcatcgtc
     8941 tttaaaaatc cgattcttat tattacagga tttgtaccag tgttctttga caatgcagcc
     9001 attgcggtct acgctgataa acgcggcgga tggaaagcgg ctgttatcct ttcctttata
     9061 tcaggtgtcc ttcaagttgc tctaggagct ctttgtgtgg cccttctcga tttggcatct
     9121 tatggtggct accatggaaa tatcgacttt gaattcccat ggcttggatt tggatatatc
     9181 ttcaaatacc ttggtattgt tggttatgta cttgtgtgtc tcttcttgct tgttattcct
     9241 caacttcaat ttgccaaagc aaaagataaa gagaaatatt acaacggtga agttcaagaa
     9301 gaagcttagt atctagaaaa ggagaaataa aatggttaaa gtattagcag cgtgcggaaa
     9361 tggaatgggt tcatcaatgg ttatcaagat gaaggttgaa aatgctctcc gtaagcttaa
     9421 tcaaacagat tttacagtca attcatgcag tgtcggtgaa gctaaaggtt tagcagtagg
     9481 atatgacatc gtaatcgctt ctcttcattt gattcaagaa ttggaagggc gaactaatgg
     9541 gaagttaatt gggcttgata acttgatgga tgataaagaa atcaccgaaa aactcagtca
     9601 agcactacag taaaaggttg gagggggctg gacagaaact gagagttatc gtttctgtcc
     9661 ttctccctct ttaaataaag gaggcagata tgaatttaaa acaagcttta attgacaacg
     9721 actcgatccg actaggttta gaggctaacg attggaaaga agcagtcaag gtagcagtag
     9781 atcccttgat tgaaagtggg gcaattttgc cagagtatta cgatgctatc attgaatcga
     9841 ctgaagagta tgggccttac tatatcttga tgccaggtat ggctatgccc cacgctagac
     9901 ctgaagctgg tgtgcaaagt gatgcctttt cattaattac cttacaaaat cctgttgtat
     9961 tttcagatgg gaaagaggta tctgttttgt tggcactagc agcaacaagt tcaaaaattc
    10021 acacaagtgt agccattcca caaattattg ccctgtttga attagaagat tctattgcac
    10081 gtttacaggc ttgccagact aaagaagatg tcttggctat gattgaagaa tctaaggata
    10141 gcccttatct cgaaggattg gatttggaaa gttagaaaga ggaataaaga aatgacaaaa
    10201 agaataccta atttacaagt tgcattagac cattcagact tgcaaggagc gattaaagca
    10261 gctgtttctg ttggtcagga agtagatatt atcgaagctg gaactgtttg cttgcttcaa
    10321 gttggaagtg aactggctga agtcttgcgt agccttttcc cagataagat tattgtggca
    10381 gacacaaaat gtgctgatgc tggtggaaca gttgctaaaa ataatgcggt tcgtggagca
    10441 gactggatga cttgtatctg ttgtgcaacc atccctacta tggaagcagc tctaaaggct
    10501 atcaagactg aacgaggaga acgaggcgaa atccagatcg agctttatgg cgattggact
    10561 tttgaacaag ctcagctttg gctagatgca ggtatctcac aagctattta tcaccaatct
    10621 cgtgatgctc ttcttgctgg tgaaacttgg ggtgaaaaag accttaataa ggttaaaaaa
    10681 ctcattgaca tgggcttccg tgtatctgta acaggtggtc tagatgtaga tactctcaaa
    10741 ctctttgaag gtgttgatgt ctttaccttt atcgcaggtc gtggaattac agaggctgtg
    10801 gatccagcag gagcagcgcg tgccttcaag gatgaaatca aacgaatttg ggggtaaatc
    10861 atggtacgtc caattggaat ttatgaaaag gcaaccccaa cacactttac ttggctagaa
    10921 cgtttaaatt ttgccaagga gttaggcttt gattttatcg agatgtctat tgacgaacgt
    10981 gacgagcgtt tagcaagact tgactggagt aaggaagaac gcttggaagt tgtcaaagaa
    11041 atctatgaaa ctggtgttcg tattccttct atctgttttt caggccatcg tcgctaccca
    11101 ttgggatcaa aagattcagt tctagaggaa aaatctctag aactcatgaa aaaatgtatc
    11161 gaattagctc aagacttggg agttcgtacg attcaattag ctggttacga tgtttactat
    11221 gaggaaaagt caccccagac acgccaacgt tttatcaaaa atttgagaaa agcctgtgac
    11281 tgggctgaag aagctcaggt ggtacttgct attgaaatta tggatgatcc tttcatcagt
    11341 agcatcgaaa aatatttggc tatagaaaaa gagattgact ctcccttcct ctttgtatat
    11401 ccagatattg gtaatgtgtc tgcatggcat aatgatatct atagtgagtt ttatcttggt
    11461 catcatgcca tcgcagctct ccatctcaag gatacttatg cagtgacaga aagttcaaag
    11521 ggccagttcc gagatgtacc tttcgggcaa ggttgtgtca aatgggaaga agctttcgat
    11581 attttaaagg aaaccaatta taatggacct ttcctaatcg aaatgtggtc tgaaaattgt
    11641 gaaacagtag aagaaacacg cgcagccatt caagaggcgc aagcttttct ctatccactc
    11701 attaagaaag caggtttgat gtaagatgaa tcaagtaatc aatgctatgc gtaaacgagt
    11761 ctgtgatgcc aatcaatcat tgccaaaaca tggacttgtc aaatttacct gggggaatgt
    11821 atctgaagtt aatcgcgaac tcggtgtcat tgttatcaaa ccatcaggcg tggattatga
    11881 cgaattgaca cctgaaaaca tggtagtgac tgatctagat ggtaagatcc tagaagggga
    11941 tttaagacca tcttccgacc tcccaactca tgtgcaatta tataaggctt ggtcagaaat
    12001 tggtagtgtg gttcacaccc attcgacaga agctgttggt tgggctcagg caggtcgtga
    12061 tattcctttc tacggaacaa cccatgcaga ttatttctac ggttcaatcc cttgcgcccg
    12121 tagtttgacc aaggacgaag tagaagtggc ctatgaaaaa gatactggcc tggttatcgt
    12181 agaagagttt gaacatcgcg gacttaaccc ggttgaagta ccaggaatta ttgtacgcaa
    12241 tcacggtcca ttcacctggg gcaaaaatcc agagaatgct gtttatcact ctgtcgtact
    12301 agaggaagta tcaaagatga atcgctttac agaacaaatc aatccaagag ttggacctgc
    12361 tccccagtac atactagaaa aacactacca acgtaaacat ggaccaaatg cttattatgg
    12421 tcaaaagtaa gaacgatgaa ggaggagaaa aagataaatt tagctcctct ttttacattt
    12481 gatttttatt gagagtaaag ttggaattga agtaatttta aaagattttt tagaaatagc
    12541 gcttgatata tatatggtaa aataaaaaga attgctgtga tatcaataga tttgggggat
    12601 tttttaatat ggtactggat aaggcaagtt gtgatttgct tcaatatttg atggatcaag
    12661 aaacgtccaa aacgattatg gcgatttcga aagatttgaa agagtcaaga aggaaaattt
    12721 attatcacat tgacaaaatc aatgctgctc tgggtgacga ggcgcttcac atcattagta
    12781 ttccacgaat tggtattcac ttaacggaag agcagagaga tgcttgttgt aaactattat
    12841 cggaagtaga ttcgtacgat tatatcatga gtgcgcatga acgtatgatg ataatgttac
    12901 tatggatagg tatttctaaa gaacgtatta cgattgaaaa attgatagag ttaacagagg
    12961 tatctaggaa tactgttctc aatgatttga atagtattcg ttatcaacta actttggaac
    13021 aatatcaggt gatcttgcaa gtgagcaagt cacagggata ccaccttcat gcccaccctc
    13081 ttaataaaat tcagtatctt caatcgcttc tatatcatat ttttatggaa gaaaatgcca
    13141 cttttgtatc tattttagaa gataagatga aagagaggtt agatgatgag tgtttgcttt
    13201 ctgttgaaat gaaccaattt tttaaggaac aggttccttt agttgaacaa gatttaggga
    13261 agaaaataaa ccatcatgaa ataactttta tgttgcaggt tctaccttat ttgctgttaa
    13321 gctgtcataa tgttgaacag tatcaagaaa gacatcagga tatagagaaa gaattttctt
    13381 tgataagaaa aagaatagag tatcaggtgt ctaagaaatt aggagaacgg ttgtttcaaa
    13441 agtttgaaat ttctttgtca ggacttgaag tttctcttgt agctgttctc ctcctctcct
    13501 atcgtaaaga tttggatatt catgcagaaa gtgatgattt tcggcaatta aaacttgctt
    13561 tagaagaatt tatctggtat tttgaatcac aaatccgaat ggagattgag aacaaggatg
    13621 atttgttacg aaatttgatg atccactgta aagccttgtt atttagaaag acttacggta
    13681 ttttttctaa aaatcctcta acaaaacaaa ttcgatccaa gtatggagaa ttatttttag
    13741 tcactagaaa atctgcggaa attttagaag gagcatggtt tattcggcta acagacgatg
    13801 atattgccta tttgacgatt catattggag gatttttaaa atatacacca tcgtctcaaa
    13861 aaaatatgaa aaaagtttat ctcgtttgtg atgaaggtgt tgcggtttcg agacttttgc
    13921 tgaaacaatg caaactttat tttccaaatg agcaaattga cactgtattt acaacagaac
    13981 aatttaagag tgtggaagat attgcacaag ttgatgtagt gattactact aatgatgatt
    14041 tggatagcag atttccgatt ttaagggtta atcctatcct tgaagcagaa gatattttga
    14101 aaatgctaga ctatcttaaa cacaatatat ttcgtaataa gagcaaaagt ttcagtgaaa
    14161 atctttctag tcttatttcg tcttatattg tagacagcaa gttggctagt aagttccaag
    14221 aagaggttca aacacttata aatcaagaaa tagtagttca agcttttttg gaagatattt
    14281 gaaggacagt ccaatgatga acacaaacct gtgtttttct tggtcttttt tagtgttttg
    14341 aagggtggta tactaatctc aaagataaca attatatcca aaggaggcaa catatgccaa
    14401 acgtcaaaga aattacaaga gagtcatgga ttttagccac tttcccagag tggggaacat
    14461 ggttgaacga agaaatcgaa gaagaagtcg tacctgaagg caactttgcc atgtggtggc
    14521 taggcaactg tggtacttgg attaagacac cagctggtgc taacgttgtc atggaccttt
    14581 ggtcaaaccg tggaaaatca accaaaaaag tgaaagatat ggttcgtggg caccaaatgg
    14641 caaatatggc aggtgttcgt aagttgcaac caaacttgcg tgttcagcca atggttatcg
    14701 atccatttgc tatcaacgaa ctagactatt acttagtttc acacttccac agtgatcata
    14761 tcgacccata cacagctgca gcaattctca acaatcctaa gttagagcat gttaagttta
    14821 tcggtcctta ccactgtgga cgaatctggg aaggatgggg tgttccaaaa gaacgtatca
    14881 tcgttgttaa accaggtgac actatcgaat taaaagatat gaagattcat gcagtagaat
    14941 catttgaccg tacttgcttg gtaactctcc cagtgaacgg tgctgatgag acaggcggtg
    15001 aacttgctgg cttggctgtt acagatgaag aaatggctca aaaggctgtt aactatatct
    15061 ttgaaacacc aggtggaacc atctatcatg gtgcagattc tcacttctca aactattttg
    15121 caaaacatgg taaagacttt aaaattgatg ttgctttgaa taactatggt gaaaatccgg
    15181 taggtatcca agacaaaatg acatctatcg accttcttcg tatggcagaa aatctgcgta
    15241 ccaaagtcat tatcccagtt cactatgata tctggtctaa cttcatggct tctactaatg
    15301 agattctaga actttggaaa atgcgaaaag atcgcttgca atacgatttc catccattta
    15361 tctgggaagt tggcggtaag tacacttatc ctcaagatca acacttagta gaataccatc
    15421 atccacgtgg ttttgatgat tgttttgaac aagactctaa cattcaattt aaagctttgc
    15481 tataatataa aaatattctt tggaggttat ccgtatgttg catgatacgg ataattttca
    15541 tataatagta aaatagaatg tgtgattcaa taatcacctc aaatagaaag gaaattctat
    15601 gtcaaatcta tctgttaatg caattcgttt tctaggtatt gacgccatta ataaagccaa
    15661 ctcaggtcat ccaggtgtgg ttatgggagc ggctccgatg gcttacagcc tctttacaaa
    15721 acaacttcat atcaatccag ctcaaccaaa ctggattaac cgcgaccgct ttattctttc
    15781 agcaggtcat ggttcaatgc tcctttatgc tcttcttcac ctttctggtt ttgaagatgt
    15841 cagcatggat gagattaaga gtttccgtca atggggttca aaaacaccag gtcacccaga
    15901 atttggtcat acggcaggga ttgatgctac gacaggtcct ctagggcaag gtatttcaac
    15961 tgctactggt tttgcccaag cagaacgttt cttggcagcc aaatataacc gtgaaggcta
    16021 caatatcttt gaccactata cttacgttat ctgtggagac ggagacttga tggaaggtgt
    16081 ctcaagcgag gcagcttcat acgcaggttt gcaaaaactt gataagttgg ttgttcttta
    16141 tgattcaaat gatatcaact tggatggtga gacaaaggat tcctttacag aaagtgttcg
    16201 tgaccgttac aatgcctacg gttggcatac tgccttggtt gaaaatggaa cagacttgga
    16261 agccatccat gctgctatcg aaacagcaaa agcttcaggc aagccatctt tgattgaagt
    16321 gaagacggtt attggatacg gttctccaaa caaacaagga actaatgctg tacacggcgc
    16381 ccctcttgga gcagatgaaa ctgcatcaac tcgtcaagcc ctcggttggg actacgaacc
    16441 atttgaaatt ccagaacaag tatatgctga tttcaaagaa catgttgcag accgtggcgc
    16501 atcagcttat caagcttgga ctaaattagt tgcagattat aaagaagctc atccagaact
    16561 ggctgcagaa gtagaagcca tcatcgacgg acgtgatcca gtcgaagtga ctccagcaga
    16621 cttcccagct ttagaaaatg gtttttctca agcaactcgt aattcaagtc aagatgcctt
    16681 gaatgttgtg gcagctaaat taccaacctt tctaggtgga tcagctgacc ttgctcactc
    16741 aaacatgact tatatcaaga cggatggact tcaagacgat gccaatcgct tgaatcgtaa
    16801 cattcagttt ggtgttcgtg aatttgcaat gggaacaatc ttgaacggga tggcccttca
    16861 tggtggactt cgtgtatacg gtggaacttt cttcgtcttc tctgactatg tgaaggcagc
    16921 tgtccgcttg tcagccttac aaggacttcc tgtgacttat gtctttaccc atgattcaat
    16981 cgcagttggg gaagatggtc cgactcacga accagttgag catttagcag gtcttcgtgc
    17041 tatgccaaat ctaaatgttt tccgtccagc agatgcgcgt gaaacgcaag cagcttggta
    17101 ccttgcagtg acaagtgaga aaacaccaac tgcccttgtc ttgacacgtc aaaacttgac
    17161 tgttgaagat ggaacagact tcgacaaggt tgctaaaggt gcttatgttg tatatgaaaa
    17221 tgcagccgac tttgatacca tcttgattgc gacaggttca gaggttaatc ttgctgtctc
    17281 agctgctaaa gaattggcta gtcaaggcga aaaaatccgc gtagtcagca tgccatctac
    17341 agatgtcttt gataaacaag atgcagctta caaggaagaa attcttccaa atgcagtccg
    17401 ccgtcgtgtt gcagtcgaaa tgggtgcaag tcaaaactgg tacaaatatg ttggtctcga
    17461 tggtgccgtt ctaggtattg ataccttcgg agcctctgcc ccagcaccaa aagtattggc
    17521 agaatatggc tttactgtag aaaatcttgt aaaaattgtt cgaaacttga aataatccta
    17581 aaaatcaggg cgtaagctct ggtttttctt accagaaaag taaggtacaa tcttgtaaaa
    17641 gtagctgaaa tttgatatag tagtcctatg taaaagacaa aggagaaaac aatgaatcca
    17701 aatattactt ttttaatcat gcttgtaggt atgatggcct tgatgttctt tatgcaacgt
    17761 tctcaaaaga aacaagctca aaagcgtatg gaaagcttaa ataaactaca aaaaggctat
    17821 gaagtgatta caatcggtgg actttatgga acagtcgatg aagtagatac ggagaaggga
    17881 acaatagttc ttgacgtaga tggagtttac ttgacttttg aactagctgc tatcaagaca
    17941 gtattaccgc tgaaagaaac agcttcacta gaaggcgcaa ttgaaaaata agacgggatc
    18001 acgaactccc gtttttctat aaaagaaagg aaatgggatg aaaaaattag tctttgtctg
    18061 tctgggaaat atttgccgta gccctatggc cgagtttgtt atgaaatcaa tgacagataa
    18121 ctacgaaatc caaagtcgag caacttcctc ttgggaacat ggcaatccga ttcataaggg
    18181 gactcaggga atttttcaac agtatgagat tctttatgac aagaacaaga catcgcttca
    18241 gattagtaag gaagattttg aagcctttga ttatattatc ggaatggacg cttcaaatat
    18301 ttctgactta cgtcagatgt gtccagtaga ctgtcaagat aagatttact cattttcatc
    18361 tgaaagtgtt ccagaccctt ggtatacagg agattttgaa gaaacctatc gacgtgttca
    18421 agagggctgt caagcttggt tagaacgttt agaaaaggag agttaaggtg gaaaatctta
    18481 cgaattttta cgaaaagtat cgtgtctatc tgactcgtcc acgtttagag cttttggcag
    18541 tagttaccat tgttttctgt gctgtactcg tcttttttct aaatattcca ggaaaaggtg
    18601 tcttaaaact cgataatgga acgattgttt atgatggcag tcttgtccgt ggtaaaatga
    18661 atggtcaagg taccattacc ttccaaaatg gagaccaata tacaggtggc ttcaacaatg
    18721 gagccttcaa cggaaaaggt acctttcaat ctaaagaagg ctggacctac gaaggtgatt
    18781 ttgtaaatgg tcaggctgaa ggaaaaggga aactaacaac agaacaagaa gtcgtttatg
    18841 aaggaacttt taaacaaggc gtttttcaac aaaaataaag cctccttatc caaaggaggt
    18901 attattagaa ccacaaggta agcgcttacc tgtaaatccc tttctttcca aatccctctt
    18961 ccaagcaagt ttgtgaaata aaaaatattt gaaataaatt tcacaaactt caaagataaa
    19021 acctgataag aaaagaaaat gagaaaagtt tcacaagagt ttaaaaattc tttgtgaaat
    19081 gtttatgaaa ctgtttacaa agttgaaata atgcgttata ataaacttgt gaaaaaatta
    19141 acaaaggata tcgttccttg aaagctatgg aggaaaatat ggctgataaa aaaactgtga
    19201 caccagagga aaagaaactc gttgctgaaa aacacgtaga tgagttggtt caaaaagctc
    19261 tagttgccct tgaagaaatg cgtaaattgg atcaagagca agttgactac atcgttgcta
    19321 aagcatcagt agcagctttg gatgcccacg gagaattggc tttacatgcc tttgaagaaa
    19381 caggacgtgg tgtatttgaa gataaagcaa ctaagaactt gtttgcctgt gaacacgtag
    19441 taaacaacat gcgtcacact aagacagttg gcgttatcga agaagacgat gtaacaggat
    19501 tgactcttat tgctgaacca gttggtgttg tttgtggtat tactccaaca acaaacccaa
    19561 catcaacagc aatcttcaaa tcattgattt cattgaagac acgtaaccca atcgtctttg
    19621 ccttccatcc atcagcacaa gaatcatctg ctcatgcagc tcgtatcgtc cgcgatgcag
    19681 ctatcgcagc tggtgctcct gaaaactgtg tgcaatggat tactcaacca tctatggaag
    19741 caacaagtgc ccttatgaac cacgaaggtg ttgcgacaat ccttgcaaca ggtggtaatg
    19801 ccatggttaa ggcggcttat tcatgtggta aaccagctct tggggtaggt gccggaaacg
    19861 ttccagctta tgttgaaaaa tcagcaaaca ttcgtcaagc agcacacgat atcgtcatgt
    19921 ctaaatcatt tgataacggt atggtctgtg catctgaaca agcagttatc attgataaag
    19981 aaatttacga tgaatttgta gcagagttca aatcttacca cacttacttt gtaaacaaaa
    20041 aagaaaaagc tcttcttgaa gagttctgct tcggcgtcaa agcaaacagc aaaaactgtg
    20101 ctggtgcaaa attgaacgct gacatcgttg gtaaaccagc aacttggatt gcagaacaag
    20161 caggatttac agttccagaa ggaacaaaca ttcttgctgc agaatgtaaa gaagttggcg
    20221 aaaatgagcc attgactcgt gaaaaattgt caccagttat tgcagttttg aaatctgaaa
    20281 gccgtgaaga tggtattact aaggctcgtc aaatggttga atttaacggt cttggacact
    20341 cagcagctat ccacacagct gacgaagaat tgactaaaga atttggtaaa gctgttaaag
    20401 ctattcgtgt tatctgtaac tcaccttcta cttttggtgg tattggggac gtttacaatg
    20461 ccttcttgcc atcattgaca cttggatgtg gttcttacgg acgcaactca gttggggata
    20521 acgttagtgc cattaacctc ttgaatatca aaaaagtcgg aagacggaga aataacatgc
    20581 aatggatgaa acttccttca aaaacatact ttgaacgtga ttcaattcaa taccttcaaa
    20641 aatgtcgtga cgttgaacgt gtcatgatcg ttactgacca tgccatggta gagcttggtt
    20701 tccttgatcg tatcatcgaa caactggacc ttcgtcgcaa taaggttgtt taccaaatct
    20761 ttgcggatgt agaaccggat ccagatatca caactgtaaa ccgtggtact gagattatgc
    20821 gtgccttcaa accagatacc atcatcgcac tcggtggtgg gtctccaatg gatgctgcca
    20881 aagtaatgtg gctcttctac gagcaaccag aagtggactt ccgtgacctt gtccaaaaat
    20941 tcatggatat ccgtaaacgt gccttcaagt tcccattgct tggtaagaag actaaattca
    21001 tcgcgattcc aactacatct ggtacaggat ctgaagtaac accatttgcc gttatctctg
    21061 ataaagcaaa caaccgtaaa tacccaatcg ctgactactc attgacacca actgtggcaa
    21121 tcgtagatcc tgctttggta ttgacagttc caggatttgt tgctgctgat actggtatgg
    21181 acgtattgac tcacgcgaca gaagcatacg tatcacaaat ggctagtgac tacactgatg
    21241 gtttagcact tcaagccatt aaattggtct ttgaaaatct cgaaagctca gttaagaatg
    21301 cagacttcca ctcacgtgag aaaatgcata acgcttcaac aatcgctggt atggcctttg
    21361 ccaatgcctt cctaggtatt tctcactcaa tggcccataa gattggtgcg caattccaca
    21421 caatccacgg tcgtacaaat gccatcttgc ttccatacgt tatccgttac aacggtacac
    21481 gtccagctaa gacagcaaca tggcctaagt acaactacta ccgtgcagat gaaaaatacc
    21541 aagatatcgc acgcatgctt ggacttccag cttctactcc agaagaaggg gttgaatctt
    21601 acgcaaaagc tgtctacgaa ctcggtgaac gtattgggat ccaaatgaat tttagagacc
    21661 aaggaattga cgaaaaagaa tggaaagaac attctcgtga attagccttc ctggcttatg
    21721 aagaccaatg ttcaccagct aacccacgtc ttccaatggt agaccatatg caagaaatca
    21781 tcgaagatgc atactatggc tacaaagaaa gaccaggacg ccgtaaataa ttgtttatca
    21841 gtctagaagc aagacaaaaa ctcaatttga gggaaagatc cagtaatttt tctatgataa
    21901 aaggcatcct atcaaggttt ttgaacacct gataggatgc ctttttatga tattgaggcc
    21961 tttttgccct ttttgaaaaa ctagaataga aacaaaatat ataatagatt gaaactagaa
    22021 tagtacacat ctgcttctaa aacattgtta gaattcgatt tgactgtcct gatcgatttg
    22081 tcctgttctt atttcatttt gatatataaa aaatat
//
LOCUS       DAVKQB010000037        21599 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKQB010000037 DAVKQB010000000
VERSION     DAVKQB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.037"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(202..1533)
                     /locus_tag="VW528_001453"
     CDS             complement(202..1533)
                     /locus_tag="VW528_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9912264.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(1555..3066)
                     /locus_tag="VW528_001454"
     CDS             complement(1555..3066)
                     /locus_tag="VW528_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9912265.1"
                     /translation="MSEKAKKGFKMPSSQTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALAIGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            complement(3275..4222)
                     /gene="arcC"
                     /locus_tag="VW528_001455"
     CDS             complement(3275..4222)
                     /gene="arcC"
                     /locus_tag="VW528_001455"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9912266.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNSGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            complement(4387..5403)
                     /gene="argF"
                     /locus_tag="VW528_001456"
     CDS             complement(4387..5403)
                     /gene="argF"
                     /locus_tag="VW528_001456"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9912267.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            complement(5461..6690)
                     /gene="arcA"
                     /locus_tag="VW528_001457"
     CDS             complement(5461..6690)
                     /gene="arcA"
                     /locus_tag="VW528_001457"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9912268.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            7446..9125
                     /locus_tag="VW528_001458"
     CDS             7446..9125
                     /locus_tag="VW528_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9912269.1"
                     /translation="MKKIKPHRPLPSQTQLAYLGDELAAFIHFGPNTFYNQEWGTGQE
                     DPERFNPTQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            complement(9165..11249)
                     /locus_tag="VW528_001459"
     CDS             complement(9165..11249)
                     /locus_tag="VW528_001459"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9912270.1"
                     /translation="MKPLLETIDTRFGTASKHAFSWGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKASAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            11420..12700
                     /locus_tag="VW528_001460"
     CDS             11420..12700
                     /locus_tag="VW528_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9912271.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKNFLLDQLVACDGGTGLMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            12791..15436
                     /locus_tag="VW528_001461"
     CDS             12791..15436
                     /locus_tag="VW528_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9912272.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            15520..16389
                     /locus_tag="VW528_001462"
     CDS             15520..16389
                     /locus_tag="VW528_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9912273.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            16383..18263
                     /locus_tag="VW528_001463"
     CDS             16383..18263
                     /locus_tag="VW528_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9912274.1"
                     /translation="MVRFIGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRHDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            18554..18916
                     /locus_tag="VW528_001464"
     CDS             18554..18916
                     /locus_tag="VW528_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912275.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNIEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(18968..19817)
                     /locus_tag="VW528_001465"
                     /pseudo
     CDS             complement(18968..19817)
                     /locus_tag="VW528_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(19762..19944)
                     /locus_tag="VW528_001466"
     CDS             complement(19762..19944)
                     /locus_tag="VW528_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9912276.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            20213..>21599
                     /gene="pcpA"
                     /locus_tag="VW528_001467"
     CDS             20213..>21599
                     /gene="pcpA"
                     /locus_tag="VW528_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU9912277.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNRGV"
BASE COUNT         6314 a         4328 c         4743 g         6214 t
ORIGIN      
        1 actttagacc agaaacatcc acaccaaatc tttttgaaag ctgtttgaag ctttgtcctt
       61 gctttcttag ttcatagatc tgaactttat cttcataact caatttcata ataaaacacc
      121 ccaaaagtta gatttattct gtctaacttt tggggtgcag ttcattttgc gggatttttt
      181 ttggtagaaa cagttgcctg attaagttgc aagtcgatag acggcttcgg cataaatatc
      241 catagcacgg tacaaatctt ctagaacggc acattcattt gcctgatgtt ctgtctgctt
      301 cgctcctggg aataaggcgc cgaaggctac acaatttggc atggtgcgag caaaagtggc
      361 accaccggat gaaatagcag gactgttatc gccagtcttt tcttggtaga tttgcatcag
      421 tgtgctaacg agtttactgt cttttgcgac gtatagaggc gctagatagt caaactcttc
      481 gtagcggagt tggtagtttt gtgcacatct tgtaagcaac tctactagtt cttccttatc
      541 agctaagaca ggagtccgaa tgtcaatacg gatttcagaa cgttcatgat tgatcatgag
      601 acctgcgaca ttaaaggata ggtgaccaga aggttcatct gctatatcac caaagatttg
      661 tcttcctgtg ccgtcttgac ctgcttgtgt tgcaagaaag ctgagagcag ggtgttcttg
      721 gagaggagca agaatggtag ctagtcggat gacagcattg ataccttgac tagcatcctt
      781 agcatgcttt ggcactccga gaaccgttac ggtttgttca gtggattggt aatcataacc
      841 agcttctttg agatcgttac aaacctgttc atagagggga ccttggtagt tggccttgtc
      901 tggtacaacg ttaaaggcgc ctcctacttc aagctctagt tgatccgatc cagggccatg
      961 aagtttgacc tgtagaagcc ctttttcagc ataggtcaga ggaaaagatg agtcaggtgc
     1021 aaagcccata ctggcctgtt cttcgatggt attgtagcgt gccatgcagc gccagagggt
     1081 ttcctcatcg gtaccaaaga taaagcgtac gcgctttttg aactgaatac cttggtccag
     1141 caagcttttt actgcataga gagctgcgag cgaagggcct ttatcatctt ggacaccacg
     1201 tccgaatacc cagccgtctt tgatagttgc ttcaaatggc ggtgtctgcc aatctgcttc
     1261 atcacctgat ggaacaacat ccaaatgaca gagaatggcc agaagctctg ctccctgacc
     1321 gatttctgca tatccgtaat aacctttagg gtcaagatag gtagtgaaac ctatgtctcg
     1381 acaaatctct aaagtttttt ctaggacatc ttggattgct tgtccaaaag gtgttccatt
     1441 ttctccttca ttgagtactg aaggatagga aatcaaggtt tttaatgata taagaaattc
     1501 atctttaact tgatttgtta tatctatttt catggaatca cctcactcac tattttatag
     1561 gaatggaagg aaggttccaa ggagaagaag ggcgatggtc actacaataa tagcgactac
     1621 gagtttgccc atgaatttcc accaagtacc aatgttgata cgtccaattg caagagctcc
     1681 catcacaata ccagatgttg gtgcaatcaa gttcaagaca cctgaagcag attggtaagc
     1741 agtgataatc aagctaggac ggacatttac aaattctcca agtggagcca tgatacccat
     1801 agttgcgctg gcaagaccag atgaagatgg gatcaagaat gacataggta gatagaagat
     1861 ataagttaca acgataaaga cttgtgaaga tagaccgctc aagccttctt taccccagtt
     1921 gaggattgta tcggtaatca taccgtcgtt catgataact tgaataccac gagcaatcgc
     1981 tacgatcaag gcaacactga gcaagtcagc agcaccattc atgaaggaag agataatctt
     2041 atcttcttta agaccataaa taacaccaat caggataccc ataaaggcaa agagcattgc
     2101 gccttctggg aagtaccaag tacctagtgc agaagtagat gaaccgacaa tattaccaat
     2161 aactggaaga ccagtcaacc aagtattaaa gtcatcaaaa atggtaacgc caaggtctgt
     2221 ccatggaatg aagctcaata ccatcaagat gaatgtcaac acaaataaga agagaactga
     2281 tttttgtttg ctgctaagtg tagattctac agatgaagat tcttctacgt taaagtgttt
     2341 caaatcttct ttgcgagtac tataaaccag tgacttagtc ggatcttttt gaatcttatc
     2401 cgcataacgg taaacaaacc aagtactaag agcagtcaag gtaacccaga agatcagacg
     2461 aagtacgata ccgtcccctg taccaactcc cgcagtcgct gaagcaatac ctgtcgcaaa
     2521 tggattcaga gtagatgcca aacagccgat ttgagaaccg agcaaaataa ttgcaacacc
     2581 agtcaggcta tcaaaaccaa cggccatcat aactggcaca aggagtggat agaaggccat
     2641 tgtttcttca cccataccat aagttgtacc accgagggca aacaaaggca tcagtaccaa
     2701 aattaacatt ttttcgcggc ccttatactt cttcacgata gaggcaatcc ctacgtcaag
     2761 agcaccagtt ttgttgacaa tgccaaggaa accaccaacc ataaggatga agaaggctac
     2821 atcaatcgct gcgctcgttt ctttaatgag cgaaccttcc tctggatgag tacctagcat
     2881 agcccgaatc ggtgccatga ggacatccca aatcccttgt ggattttgag gctgagtctc
     2941 gtaaatacct tctataaagg cccccgcagg gataaaccaa gttagcactg ccataatagc
     3001 aatgattatc aataatacgg tttgagatga aggcatctta aacccttttt tagctttttc
     3061 actcatttgt ttttcctcct gatgtctaac aaagtgtaaa aaaccaacta ctttcttaaa
     3121 taaacattca agaaaacgct ttctatttat atttgtttta ccatattaaa gtctaaaact
     3181 agtatctttt gaaatattgt acatgttttc aagaataatt tctaatttga tataaagata
     3241 ctagaaatag aatacatctt attagtaaaa caacttatcc tttttcaata attgttccgc
     3301 tttcagattc aatcaaggcg cctagatttt caagggaagt aataactgct tttccttctg
     3361 gacgaccatt gacaaaagcg atagctgctt ctacttttgg aagcatgcta cctggtgcaa
     3421 actgatcttg tttgatatat tcttccagct gggcaacatt cacatgttcc aatttttcct
     3481 ggtttggctt gttgtagtta acaaatacat aatctacacc tgtcaaaacg atgaagaggt
     3541 ctgcatcaac caattctgcc aaacgttggg aagcgaagtc tttatcaata accgcttcga
     3601 caccagtcaa atgtccattg ttttctttga cgacgggaat accgccacca cctgcagcta
     3661 cgacgacttg accactattt aaaagagtac ggatggtttc aatttctttg atgtcaacag
     3721 gttttggtga ggcaacgacc ttacgccagc cacggccagc atcttccttg aaagtcgctc
     3781 cgctcttttc ggcttctgct tttgcttctt cttctgaata gaaaggaccg attggtttac
     3841 tcaagttaac aaaagctgga tcatttttat ctacgacaac ttgcgttaca acagaggcaa
     3901 cgtttttttc gatgccttca tccaagagag cattttgcaa agcatttttc aaccagaaac
     3961 cgatgctacc ttctgtcata gcgacaagtg agtcgagtgg gaaggcaggg ttcttttcag
     4021 agtctgatgc caaatgttgg agcaagagat tcccaacttg aggtccatta ccgtgagtga
     4081 taatcagatc atctccattt ttaatcaatt ttacaagatg cttagctgtt tcaactaaag
     4141 cttcttgttg agcctttgct gatgggtcag aagaaagaat cgcatttcct cccaaagcta
     4201 ctacaatttt acgatttgcc ataaattctc ctttatcaca ctcaatagaa tgcgtttaga
     4261 tttcaattta atgatttttc acatatttta taagaaataa tagattacca ttacataaaa
     4321 gaggaccgga ctaaagctat tagtcgcagc cctcatagct gttggtagac ggtttattat
     4381 ctaaaattat actttaggaa tataaaggtt accaagtgtc gcagccataa cagctttgat
     4441 agtgtgcata cggttttctg cttgatcgaa gtggcgagcg tacttgctgc ggaagacttc
     4501 gtctgttact tccatttctt ctacaccaaa tttttcagca acgtctttac cataaacagt
     4561 gtgagtatcg tggaatgctg gcaagcagtg taggaagatc aagttttcat tgcctgcttt
     4621 tttaactaag tccatattga cttggtaagg tttaagaaga gctacacgtt ctgcgaattt
     4681 gtcttcttca cccattgata cccaaacgtc tgtgtaaaga acgtctgcat ctttaactgc
     4741 ttcatcagca tcttcagtga tgagaacatg tgcgccactt tctttagcaa atccttctgc
     4801 caattcaacg atttcttttt ctgggaagag ttcttttggt gagaagatgt gaacattgac
     4861 accaaggata gcacctgtta cgagcaagct gttggcaacg ttgttacgtc catcaccaca
     4921 gtataccaat gtcaagcctt ccaagcgacc gaagttttct tgaacagtca agtagtcagc
     4981 gagcatttga gttgggtgcc attcgtcagt tagaccgttc catactggaa cgcctgagaa
     5041 ttctgccaat tcttcaacca tacgttggct gaatccgcgg aattcaatcc cgtcaaacat
     5101 acgtcccaat actttagcag tatcttcagt agattctttt ttacccaact gaatatcatt
     5161 tgctccgagg tattctgggt gagcaccaag gtcgatagcc gcagttgtaa aggctgcacg
     5221 agtacgagta gatgtttttt caaataggag agcgatattc ttgccagcaa ggtagtggtg
     5281 ttgaatattg cgttttttca aatctttcaa gtgagctgaa agaccaataa ggtattctaa
     5341 ctctgcacgg gtaaagtctt tttctgctaa gaagctgcgt ccttggaata ctgaatttgt
     5401 cattttatta tttcctcttt ctatttttta cattttctat tgacgaatgc cgaacagcga
     5461 ttacacttct tcacgttcaa atggcataga catacaacga ggtccaccac ggccccgaac
     5521 caattcactt ccgcgaatct taatcaagcg aagcccgtat tcttccaaaa tcttattggt
     5581 cacggtattg cggtcataaa caactaccac accaggtgcg atggtcaaag tgttagaacc
     5641 gtcgttccat tgttcacgcg cagctgctac gatattgcca ccaccgcaac gaatcaaatg
     5701 aactttttct acaccaaggt tttgagcaag aagttcagct aagtcacctt tctcttcaac
     5761 gattttaagt ttttcgtttt cgtaagtaac tgagtaaacg tgaaggtcgc cttcgatttc
     5821 tgggtgaata gtgaacttgt catagtctac catagtgaag acagtatcca agtgcatgaa
     5881 tttacggttg ttagcaaatt caaaggccaa aactttcttg aagccaacat ttttcttgaa
     5941 gatgttgacc aaaagttttt cgatagaagc tgcgtctgta cgttgagaga tacctactgc
     6001 aaggacgtct ttagaaagaa ctagctcgtc tccaccttcg atacgcgtat cttcttcacg
     6061 gttgtagacc aaatccactt ttccgccata gattgggtgg tatttgaaga tatacttacc
     6121 gtagagtgtt tcacggttac gagtgtctgc aaacatgtgg ttaagcgata cggcgtttcc
     6181 aattgttgca aatgggtcgc gagtgaaata gaggtttggc atcgggtcaa ttgcaaatgg
     6241 ataatctgat tcaactaagt cagttagatc tttagcttcg tcaggaattt ctggcaattc
     6301 aactttttga atcccagcca ttgttttttc aaccaattct tggttgtcct tgatgccgtg
     6361 aagcaattca cgaatagcaa ccttggtttg acgatcacgg atgttggctt cgtctaagta
     6421 ttcctcgata aattgatcgc ggatttctgg agaggtcaat gattcagcag cgagttgttc
     6481 taggtagaga acctcaattc cttcatcgcg aagagcttgg gcaaatgcat catgttcttt
     6541 ttgagcatct tccaagaaag gaatatcatc aaaaagaagc ctttcaagat agtccggcaa
     6601 caagttttct aactccttgc ctggacggtg caacataact tttttcagtt tcccaatttc
     6661 tgagaagacc tgaattggat gagaagacat gtattttcct ccttaaaaat tttgacttgg
     6721 caccattttg ggtaccgctt acatgtatgt tataacgcaa ttcctaggta atgtcaatgg
     6781 ctataatttc tcggaaaata atataaatta tgatgtttag cacaatttta tgttattata
     6841 cataacatag gatagaatac atagatacaa atgcataaac ttctattaaa gaggatataa
     6901 aattttttta aaaaattttt caaaaaatat acaaatgact aaaaagtgtc caattatcat
     6961 ctggttatca cttggatctg tataaagaga aatgtttgtg tgaaaggata aaaagatgca
     7021 acattctttt gaagatagat tttggagaag catttcttgt tcaattgtac aaattataga
     7081 atcttttctg agtatttagg aagagataat tatcttattg accctctttt tattttttgt
     7141 tatagtcgac gaggtgatag tttagaataa tcacttattc acaatctgtg gaaaagtctg
     7201 gagagtgaat ggtcttcaca caatgacctg aaagaagcct gagaataatt atggagagta
     7261 gcattctgag aggtgttagc agaaccatat gacagagctg tttgaagagg gaatattgag
     7321 gagaaaaatc ctgagcctac cagttggagt tggaaagagc tggctgttag atcatggttt
     7381 attatccaca acctgtggat aaccttgtga acaagagaag ttgcaaaaga aggagatata
     7441 taacgatgaa gaaaatcaaa ccgcatagac cgttaccaag tcagactcag ctagcttatc
     7501 tgggagatga actagcagcc tttatccatt ttggacctaa tactttttat aatcaggaat
     7561 ggggaacagg tcaggaggat cctgaacgct ttaacccgac acagttagac gcacgtgagt
     7621 gggttcgcgt gctcaaggaa acgggcttca aaaagttgat tttggtggtc aagcaccacg
     7681 atggctttgt cctttatccg acagctcaca cagattattc ggttaaggtc agtccttgga
     7741 ggagaggaaa gggcgacttg ctccttgaag tatcccaagc tgccacagag tttgatatgg
     7801 atatgggggt ctacctgtca ccgtgggatg cccatagtcc cctctatcat gtggaccgag
     7861 aagcggacta caatgcctat tatctggctc agttgaagga aatcttatca aatcctaact
     7921 atgggaatgc tggtaagttc gctgaggttt ggatggatgg tgccagagga gagggcgcgc
     7981 aaaaggttaa ttatgaattt gaaaaatggt ttgaaaccat tcgtgacctg cagggcgctt
     8041 gcttgatttt ttcaacagaa ggcaccagta tccgctggat tggcaatgaa cgagggtatg
     8101 caggtgatcc actgtggcaa aaggtgaatc ctgataaact aggaacagaa gcagagctga
     8161 actatcttca gcacggggat ccctcgggca cgattttttc aatcggagag gcagatgttt
     8221 ccatccgtcc aggctggttc taccatgagg atcaggatcc taagtctctc gaggagttgg
     8281 tcgaaatcta ctttcactca gtagggcgag gaactccact cttgcttaat attccgccga
     8341 atcaagctgg gctctttgat gcaaaggata ttgaacgact ttatgaattt gcgacctatc
     8401 gcaatgagct ctataaagaa gatttggctc tgggagctga ggtatctggt ccagctcttt
     8461 ccgcagactt tgcttgtcgc catttgacag acggccttga gaccagctct tgggcaagcg
     8521 atgcagactt gcccatccag ttagaactcg acttaggttc tcctaaaact tttgatgtaa
     8581 ttgagttaag agaagatttg aagctagggc aacgaatcgc tgcttttcat gtgcaagtag
     8641 aggtggatgg tgtctggcag gagtttggtt cgggtcatac tgttggttac aaacgtctct
     8701 tacgaggagc agttgttgaa gcacagaaga tacgtgtagt cattacagaa tcacaggctt
     8761 tgcctttgtt gaccaagatt tcactctata aaactccgag gttatcacaa acagtagctg
     8821 ttcaggggct agcatttgca gaaaaaagcc tagctgtggc aaagggagag actcttcatt
     8881 ttaggattga acgtagtgaa agtagctcct ctttagaggc taagatttcg attcaaccgg
     8941 ggacaggtgt ccatggtgtc gcctatcagg atgagattca agtccttcaa ttccaagcag
     9001 gggaaagcaa aaaagatttg catctaccaa ccctgtattt cgcaggagat aaaaccttgg
     9061 atttctatct gaacctaacg gtggatggtc agcttgtgga tcaacttcaa gtccaagttt
     9121 cataaaagaa gaacctttgc gcgatgcaaa ggttcttttg gttattagtg acttggtaac
     9181 cagctgaggg tgaaagttag ttgttcagct tttaagaggt cttggtgttg aatagttgat
     9241 acgagtgttt tgtccagtcg gcattctttg acaaagttaa aatggttgtg gttttgttta
     9301 gtatggatat ccagccattt atcttcttta gcgaggtaga ctcgtagatg gtcaaagaga
     9361 gggattccga ggtcatagct tggttttcct ggacaggttg gataaaatcc gagagctgac
     9421 cagatgtacc aagcagagag actaccattg tcttcatctc caggataggc ttcccaactt
     9481 gggtgaaaag ctttctgacg gagcgtcttg ataagaaggg cagtgtagtc agggtaatcg
     9541 ctgtagcgga agagataagg aatgtggaaa ctaggctggt tggaaatggc tatttgtcca
     9601 aaaggagcag tagccatctc gctcatttcg tgaatttcgt aaccatagcc tgttgtttca
     9661 aagaggggag catcttgaca ggctttcaaa agatagttgc taaaggtttc ttttccaccc
     9721 atcagttgga ttaagccagg gatgtcgtgg agaacgccta aagtagcttg aatggcagag
     9781 cattcagcgt agtctcgccc ccaactataa ggagagaagt cagggtgaaa gtttccttga
     9841 ttgtctcgtg ctcgcatgta acctgtctca gcgtcaaata gctggcggta attttgtgaa
     9901 gcagccttgt aggtttcagc gatttctatg ttctctagtt ttttggcaca gctggcgata
     9961 caaaagtcac tataggcata gtctagagta tggctaacac tttcgtggtg gtcggtagag
    10021 aggtaaccta gttcttggta ttgggctagt ccgtggcggc cattgattcc gagagggtcg
    10081 gctttgctgg ctgtttcgag catggcttgg aagagttctc cttctaggtc aggggccatg
    10141 tccttgcagg cgctatctgc gataataccg tctaaaagtg tacctggcat cataccccgt
    10201 tcatctggag ccagccattt tggaaggaaa ccagtatcgc ggtagctatt gaggaaacct
    10261 tctaaaaagc gttgatagtg ctccggtatg ataagggcaa agagggggaa ggtggtgcgg
    10321 aaggtatccc agaaaccatt gttgctaaag aggacaccag gcttgacagt accagtagcc
    10381 agatccatgt ggatggcttg ccctgactca tcaacctcat aaaaagtctg tgggaagagg
    10441 aagagtctgt agaggcagtg gtcaaagaag gttcggtcag cctctcctgt ctctataatg
    10501 tcaaaacgat ggaggagatt ttcccaatcc gcttgggcac ttgctttaca gctatcaaaa
    10561 tcttcttgag gtagattaag cagtgcttga gaaggagaga tgaaagaagt ggctagttgc
    10621 atctcggttt gactacttgc taagtcaatt cgccagtccc cagcttcttg gctgatagca
    10681 agaatatccg tgttcatttg cagggcagtg aacatcgtta gcgaattttt gttggtttca
    10741 gttttacctt cttgtcgcag ggcaagagtt cgcttatcta cttgctctac tgtcagttca
    10801 tctgttgcgt gaagatagag ggagagggct ttgccttgct tttgcttcaa acgaatagaa
    10861 gcaccgtagc aagtcggtgt gagctgggtt tcaatctgat aacgcagaga aaagagcttc
    10921 aaatagtgag gctggaagca agctttatct atatcataag aagactggcg gtgaaagagg
    10981 ctgtctcccc ccagttgact ggtgacaggt gtcagaagga gccaagagta gtccccaatc
    11041 caaggactgg gctggtgagt taatcgaatc ccctgaaaga taggcagatg cggatcaaaa
    11101 aaccaagatc catcctggtc actggtctgg ggcacaaagt aattcatccc aaaaggcacg
    11161 cctgtgtatg gcagggtatt tccccaagag aaggcatgct tgctagcagt accaaagcgg
    11221 gtatcgatgg tttcaagtag tggtttcata gtctttcctt tagctgtttt tctacattat
    11281 atcagaaaaa gagggacttt agaaaaggag tttcaaaaat aactattttg tagaaaaatg
    11341 actatcattt gtgtatgtat tttctgtctc aaaagctata tactgaacat gaaacttgtt
    11401 tgaaagaggg aattgcacga tgatttattc gaaagaaatt gttagagaat ggctagatga
    11461 agtagcagag cgggctaagg accatccaga gtgggtggat gttttcgagc gttgctacac
    11521 cgataccttg gacaatacgg ttgaaatctt agaagatggt tcaacttttg tcttgactgg
    11581 ggatattcct gctatgtggc ttcgggattc gacagcccag ctcagaccct acctgcatgt
    11641 agctaaaaga gatgccctcc tgcgtcagac cattgcaggt ttggtcaaac gtcagatgac
    11701 cttggtactc aaggatcctt atgccaattc cttcaacata gaggaaaact ggaaggggca
    11761 ccacgagacc gaccacaccg accttaatgg ctggatttgg gaacgcaagt atgaggtgga
    11821 ctcgctttgc tatcctttgc agttggctta tctcctctgg aaagaaactg gcgagactag
    11881 tcagtttgat gagacttttg tcgcagcgac taaggaaatt ctccatctat ggacagttga
    11941 acaagaccac aagaactctc cttatcgttt tgtccgagat acggaccgta aggaagacac
    12001 cttggtaaat gatggctttg gacctgactt tgcagtgaca ggtatgactt ggtcagcctt
    12061 tcgtccgagt gatgactgtt gccagtatag ttacttgatt ccgtcaaata tgtttgctgt
    12121 agtagtcttg ggttatgtgc aagaaatctt cgcagcatta aacctagctg atagccagag
    12181 tgttattgct gatgccaagc gtcttcagga tgaaatccaa gaaggaatcg aaaactacgc
    12241 ttacaccacc aacagtaagg gtgaaaagat ttatgccttt gaagtggatg gcctaggaaa
    12301 tgccagcatt atggatgatc caaacgtacc aagtctacta gctgcgccct atctgggcta
    12361 ctgttcggtc gatgatgaag tgtatcaagc tactcgccgt accattctga gctctgaaaa
    12421 tccatatttc taccaaggag aatacgctag cggtcttgga agttctcata ccttctatcg
    12481 ctatatctgg ccaatcgccc tttctatcca aggcttgaca acaagagata aggcagagaa
    12541 aaacttcttg ctggatcagc tggttgcctg cgatggcggt acaggtctta tgcatgaaag
    12601 cttccacgtg gatgacccaa ctctctactc tcgtgaatgg ttctcctggg ctaacatgat
    12661 gttctgtgag ttggtcttgg attacttgga tattcgctaa ggggctcgct ttagctcaac
    12721 cgattcttat cagaatcaca agtttacatt taaaacgtta aaatttaaat ttagaatgag
    12781 gttttacttc atggaaaatg ttgttgtaca tattatctca catagtcact gggaccgtga
    12841 gtggtacttg ccttttgaaa gtcatcgtat gcagttggtg gaattgtttg acaatctctt
    12901 tgatctcttt gaaaatgacc ctgagttcaa gagtttccac ttggatggac aaactattgt
    12961 ccttgacgac tacttacaaa ttcgccctga aaatcgcgac aaggtccaac gctacattga
    13021 cgagggcaag cttaaaattg gtccctttta catcttgcag gatgattatt tgatttcaag
    13081 tgaagccaat gtccgcaata ccttgattgg tcaacaagaa gctgccaaat ggggtaaatc
    13141 aacccagatt ggctactttc cagatacctt tggaaatatg ggacaagcac ctcaaattct
    13201 tcaaaaatca ggcattcatg tggcggcctt tggtcgtggt gtgaagccga ttggatttga
    13261 caaccaagtc cttgaagatg agcagtttac atcccagttt tcagaaatgt actggcaggg
    13321 tgtggatggt agtcgtgttt taggtattct ctttgccaac tggtacagta atgggaatga
    13381 aatcccagtt gacaaagatg aggccttgac cttctggaaa caaaaattgt cagatgtgcg
    13441 tgcctacgct tcgaccaacc aatggttgat gatgaacggc tgtgaccacc agcctgtaca
    13501 gaaaaatctg agcgaagcca ttcgtgtggc aaatgaactc ttcccggatg taatctttgt
    13561 tcatagttct tttgatgaat atgttcaagc tgtagaaggt gcgcttcctg aacacttatc
    13621 aactgttaca ggcgagttga ccagtcagga aacagatggc tggtacacac ttgccaacac
    13681 ttcttcatcc cgcatttacc tgaaacaagc cttccaagaa aatagcaacc tcctagagca
    13741 agtggtagaa cccttgacta ttatcactgg tggacacaac cacaaggacc agttgaccta
    13801 tgcttggaaa acacttttgc agaacgcgcc acatgatagt atctgtggct gtagcgtgga
    13861 cgaagttcac cgcgagatgg aaacgcgttt tgccaaggtc aaccaagtag gaaactttgt
    13921 taaaagtaac ttgctcaacg agtggaaggg taaaattgct acggataagg ctcaaagtga
    13981 ctatctcttt actgtcatta acacaggctt gcatgataag gtcgatactg tcagcacagt
    14041 gattgatgtg gcgacttgtg atttcaagga attgcaccca acagaaggct acaaaaagat
    14101 ggctgctctt atcttgccaa gttaccgtgt ggaggacttg gatggtcgtc ctgtagaggc
    14161 tacaatcgaa gacctcggag ctaattttga gtacgattta ccaaaagata agttccgcca
    14221 agctcgtatt gctcgacaag tgcgcgtgac cattccagtt cacctagcgc cgctttcttg
    14281 gacaaccttc caattgctgg aaggaaaaca agaacaccgt gagggtattt accaaaacgg
    14341 agtgattgat acaccattcg taacggtgag tgtggatgac aacatcacag tctatgacaa
    14401 gacaactcac gaagcctatg aagactttat ccgctttgaa gaccggggtg acatcggaaa
    14461 cgagtatatc tatttccaac caaaaggaac agagccaatc tttgcagagc ttaagggcca
    14521 cgaggtcttg gaaaacacag atcgctatgc taaaatcttg ctcaaacatg aattgaccgt
    14581 gcctgtcagt gcggatgaaa agctagaaga agagcaacaa ggtatcatcg agtttatgaa
    14641 gcgtgaggct ggacggtcag aagaattgac aaacattcct ctggaaactg agttgattgt
    14701 ctttgttgac aatccacaaa tccgcttcaa gactcgcttt actaacactg ccaaggatca
    14761 ccgtatccgt ctcttggtca agactcataa cacgcgtcca agcaatgatt ctgaaagtat
    14821 ctatgaggtg gtgacacgac caaacaaacc agctgcttca tgggaaaatc ctgaaaatcc
    14881 tcaacaccaa caagcttttg tcagtctgta tgacgatgaa aaaggggtga ctgtatccaa
    14941 caagggattg aatgaatacg aaatccttgg ggataacacc attgccgtga ccattttgcg
    15001 tgcatcaggt gagctaggtg actggggcta cttcccaacg ccagaagcac aatgcttgcg
    15061 tgagtttgaa gtcgaatttg cgattgaatg ccaccaagcc caagaacgct tctcagccta
    15121 tcgtcgtgcc aaagccttgc agacaccgtt taccagcctt cagcttgcta gacaggaagg
    15181 aagcgtggtt gcgactggta gcctcttgag ccattctgtt ctcagcatac cgcaagtttg
    15241 tccaacagcc tttaaagtgg cggaaaatga agaaggctat gtcctccgtt actataatat
    15301 gagtcaagaa aatgtgcgca tatcagaaca tcaacaaacc attcttgact tacttgagcg
    15361 accatatcca gttcattcag gactattggc tccacaagag attcgtacag aattcatcaa
    15421 aaaagaagaa atttaatttc aaaaagtaaa catcaaaaga aaggaggggc gaaaaagtaa
    15481 gaactaactg ctgattcgcc cctttttatg gtaaaaacaa tgaccattgc aacgattgat
    15541 atcggaggga ctgggattaa gtttgccagt ctgactcctg atgggaaaat actggataag
    15601 acaagtattt caacgcctga aaacttggag gatttactag cgtggctaga tcaacgcttg
    15661 tcagaacagg attacagtgg gattgctatg agtgttccag gtgcagtcaa tcaagagaca
    15721 ggtgtgattg atggcttcag tgcggtgccc tacatccatg gcttttcttg gtatgaggcg
    15781 cttagctctt atcagctacc tgtccattta gaaaatgatg ccaactgcgt tggactcagt
    15841 gaactactag ctcatccaga gcttgaaaat gcagcctgtg tcgtgattgg gacagggatt
    15901 ggcggagcca tgattatcaa tggtagactt catcgaggtc gccacggtct gggtggagaa
    15961 tttggctaca tgacaaccct tgcccctgct gaaaaactta ataactggtc gcaactagca
    16021 tcaactggga atatggtacg atacgtgatt gaaaaatctg gtcatactga ttgggacggt
    16081 cgcaagattt accaagaggc cgcagctggt aatgctcttt gtcaagaagc cattgagcgc
    16141 atgaaccgca atttgacgca aggcttgctc aatatccagt atctcatcga tccagatgtc
    16201 atcagtctgg gtggctctat cagtcaaaat ccagatttta tccaaggtgt taagaaggct
    16261 gttgaagact ttgtcgatgc ctacgaagaa tacacggtcg caccagttat ccaggcctgc
    16321 acctatcacg cagatgccaa tctctacggt gctcttgtca actggctaca ggaggaaaag
    16381 cagtggtaag atttatagga cttagtccca aacaaacgca agctattgag gttttaaaag
    16441 gtcacatttc tctaccagat gtggaagtgg ctgtcactca gtctgaccaa gcatctatct
    16501 ctatccaggg tgaggaaggt cactatcaat tgacctatcg caaacctcac caactttatc
    16561 gtgccttgtc cttgttggta acagttctag cagaagctga taaagtagag attgaggaac
    16621 aagcagctta cgaagatttg gcttacatgg ttgactgttc tcgaaatgcg gtgctgaatg
    16681 tggcttctgc caagcagatg attgaggtct tggctctcat gggctactca acctttgagc
    16741 tttacatgga agacacttac cagattgaag gacagcctta ctttggctat ttccgtggag
    16801 cttattcagc agaggagttg caggaaatcg aagcctatgc ccaacagttt gacatgacct
    16861 ttgtaccatg catccagacc ttggcccact tgtcggcctt tgtcaaatgg ggtgtcaagg
    16921 aagtgcagga gctccgtgat gtagaggaca ttcttctcat tggcgaagaa aaggtttatg
    16981 acctgattga tggcatgttt gccacgttgt ctaaactgaa gactcgcaag gtcaatatcg
    17041 ggatggacga agcccacttg gttggtttgg gacgctacct gattttgaac ggtgttgtgg
    17101 atcgtagtct cctcatgtgc caacacttgg agcgcgtgct ggatattgct gacaaatatg
    17161 gtttccactg ccagatgtgg agtgatatgt tcttcaaact catgtcagcg gatggccagt
    17221 acgaccgtga tgtggaaatt ccagaggaaa ctcgtgtcta cctagaccgt ctcaaagacc
    17281 gtgtgactct ggtttactgg gattattatc aggatagcga ggaaaaatac aaccgtaatt
    17341 tccgcaatca tcacaagatt agccatgacc ttgcatttgc agggggagct tggaagtgga
    17401 ttggctttac acctcacaac cattttagcc gtctagtggc tatcgaggct aataaagcct
    17461 gccgtgccaa tcagattaaa gaagtcatcg taacgggttg gggagacaat gggggtgaaa
    17521 ctgcccagtt ctctatccta ccaagcttgc aaatctgggc agaactcagc tatcgccatg
    17581 acctagatag tttgtctgcg cacttcaaga ccaatactgg tctaacggtt gaggatttta
    17641 tgcagattga ccttgccaac ctcttaccag acctaccagg caatctcagt ggcatcaatc
    17701 ccaaccgata tgtcttttat caggatgttc tctgtccgat acttgaccga cacatgacac
    17761 ctgaacagga caaaccgcac ttcgctcagg ctgctgagac gcttgctaac attaaagaaa
    17821 aagctggaaa ctatgcttat ctctttgaaa ctcaggccca gttgaatgct attttaagta
    17881 gcaaagtaga tgtgggacga cgcattcgtc aggcctacca agcggatgat aaagaaagtt
    17941 tacaacaaat cgccagacaa gaattaccag aacttagaag ccaaattgaa gacttccatg
    18001 ccctctttag tcaccaatgg ctgaaagaaa acaaggtctt tggtttggat acagttgaca
    18061 tccgtatggg cggactcttg caacgcatca aacgagcaga aagccgtatc gaggtttatc
    18121 tggctggcca gcttgaccgc atcgacgagc tggaagttga aatcctacca tttactgact
    18181 tctacgcaga caaggatttc gcagcaacta cagctaacca gtggcatacc attgcgacag
    18241 cttcgacgat ttatacgact taatactctt cgaaaatctc ttcaaaccac gtcagcttcc
    18301 atctacaacc tcaaaacagt gttttgagca gcctgcagct agcttcctag tttgctcttt
    18361 gattttcatt gagtataaaa acaagaacac cttgcttggc gcagggtgtt tcgcgtgaaa
    18421 cagaagaatt atctggtttc aaatgctaca gttaaaatta gtaaaacaag aaatagaagc
    18481 tgaaaagcca gcatctgtag aggcttggat catttccgtc aaatttaaaa aaggttgcta
    18541 ccgacatata tagattccaa aaacaaaaac gttagcggaa ctagcagatg tgattttatg
    18601 gagttttgat tttgcaaatg atcatgctca cgcatttttc atggataata ttgagtggag
    18661 tcatgcagat tcttactttc ttagctttgt tagtgacgat gttgaagaac gttacacaga
    18721 aaatgtctat ctggatagcc taagtgtcaa acaaaaattt aagtttattt tcgacttcgg
    18781 tgatgaatgg cgttttgaat gccaagtgct gagagaaatc gagacagagg acgaagaagc
    18841 ttatctcgta cgttcggttg gaacgtcgcc agaacaatat ccagattatg atggttttga
    18901 ctatgaagaa tggtaaaatt gaaatcagtc tgtgtaggct tagtatttca atagacttcc
    18961 tgcaaaacta gaatcctagt tcatgattga taataccagc aatcaaattc attcgtaatc
    19021 cgaagcgttt acgatgattt cgataggttg ttgaaaacat tttaaacgtt tttactttgg
    19081 caaagatgtt ctcaaccttg cttctctcct tggatagcgc atggttatag gctttatctt
    19141 cagctgttag tggcttgagt ttgctggatt tacgtgaagt ttgtgcttga ggacatatct
    19201 tcatgagccc ttgataacca ctgtcagcca agattttacc aacttgtctg atatttctgc
    19261 aactcatttt gaacaacttc atatcatgac aatagttcac agtgatatcc aaagaaacaa
    19321 ttctcccttg acttgtgaca atcgcttgag ccttcatagc gtgaaatttc tttttaccag
    19381 aatgattcgc taattgtttt tttagggcga ttgatttgta cttccgtcgc atcaatcatt
    19441 accgtgtcct cagagctgag aggagttctt gaaatcgtaa caccactttg aacaagagtt
    19501 acttcaaccc attggctccg acggattaag ttgctttcgt ggataccaaa atcagccgca
    19561 atttcttcat aagtgcggta ttctcacaca tattgaagag tggccataag aaggtcttct
    19621 aggcttaatt taggttttcg tccacctttt gcgtgtttaa gttgataagc tgtttttaat
    19681 acagctaaca tctctttaaa agtcgtgcgc tgaacaccaa caagacgctt aaatcgtgta
    19741 tcagttaatt gtttacttgc ttcataattt cgcagggagt ctattgactc tttggtaggt
    19801 gtcaatgttt ttttcatcta tcccgagaat tattttcccg ccatttgtat ttgcaaatgc
    19861 tgagtaggtt tcccagaaag actctggaag attgttttta gcttttttgt attctaaatc
    19921 aaccccttca aattttaagt ccatattttt cctttacatc tgttttttgt ggttctggta
    19981 tttgttcaag ttgagtgata atatagcgaa ttgaatttcg agagttttta ctcagttaat
    20041 tttccttgac ttaaaattta aaaatgatat actgtgttta tggagagatg acaatttata
    20101 aaatgtacta ctctattcta ctagataatg attgaataaa actttttata acaacggcta
    20161 gcaaagttat aattttatat ctattagaaa ataaatatgt aaggaaattt aaatgaaaaa
    20221 aactacaata ttatcattaa ctacagctgc ggttatttta gcagcatatg tccctaatga
    20281 accaatccta gcagatactc ctagttcgga agtaatcaaa gagactaaag ttggaagtat
    20341 tattcaacaa aataatatca aatataaggt tctaactgta gaaggtaaca taggaactgt
    20401 tcaagtgggt aatggagtta ctcctgtaga gtttgaagct ggtcaagatg gaaaaccatt
    20461 cacgattcct acaaaaatca cagtaggtga taaagtattt accgttactg aagtagctag
    20521 tcaagctttt agttattatc cagatgaaac aggtagaatt gtctactatc ctagctctat
    20581 tactatccca tcaagcataa aaaaaataca aaaaaaaggc ttccatggaa gtaaagctaa
    20641 aactattatt tttgacaaag gcagtcagct ggagaaaatt gaagatagag cttttgattt
    20701 ttctgaatta gaagagattg aattgcctgc atctctagaa tatattggaa caagtgcatt
    20761 ttcttttagt caaaaattga aaaagctaac cttttcctca agttcaaaat tagaattaat
    20821 atcacatgag gcttttgcta atttatcaaa tttagagaaa ctaacattac caaaatcggt
    20881 taaaacatta ggaagtaatc tatttagact cactactagc ttaaaacatg ttgatgttga
    20941 agaaggaaat gaatcgtttg cctcagttga tggtgttttg ttttcaaaag ataaaactca
    21001 attaatttat tatccaagtc aaaaaaatga cgaaagttat aaaacgccta aagagacaaa
    21061 agaacttgca tcatattcgt ttaataaaaa ttcttacttg aaaaaactcg aattgaatga
    21121 aggtttagaa aaaatcggta cttttgcatt tgcggatgcg attaaacttg aagaaattag
    21181 cttaccaaat agtttagaaa ctattgaacg tttagccttt tacggtaatt tagaattaaa
    21241 agaacttata ttaccagata atgttaaaaa ttttggtaaa cacgttatga acggtttacc
    21301 aaaattaaaa agtttaacaa ttggtaataa tatcaactca ttgccgtcct tcttcctaag
    21361 tggcgtctta gattcattaa aggaaattca tattaagaat aaaagtacag agttttctgt
    21421 gaaaaaagat acatttgcaa ttcctgaaac tgttaagttc tatgtaacat cagaacatat
    21481 aaaagatgtt cttaaatcaa atttatctac tagtaatgat atcattgttg aaaaagtaga
    21541 taatataaaa caagaaactg atgtagctaa acctaaaaag aattctaatc ggggagtag
//
LOCUS       DAVKQB010000038        21401 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKQB010000038 DAVKQB010000000
VERSION     DAVKQB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.038"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(438..2165)
                     /gene="ezrA"
                     /locus_tag="VW528_001468"
     CDS             complement(438..2165)
                     /gene="ezrA"
                     /locus_tag="VW528_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9912278.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPPQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2247..4193)
                     /gene="gyrB"
                     /locus_tag="VW528_001469"
     CDS             complement(2247..4193)
                     /gene="gyrB"
                     /locus_tag="VW528_001469"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9912279.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVYVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4208..4771)
                     /locus_tag="VW528_001470"
     CDS             complement(4208..4771)
                     /locus_tag="VW528_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9912280.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4856..5410)
                     /locus_tag="VW528_001471"
     CDS             complement(4856..5410)
                     /locus_tag="VW528_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9912281.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(5421..6644)
                     /locus_tag="VW528_001472"
     CDS             complement(5421..6644)
                     /locus_tag="VW528_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9912282.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6681..9131)
                     /locus_tag="VW528_001473"
     CDS             complement(6681..9131)
                     /locus_tag="VW528_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9912283.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVVLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9331..10260)
                     /locus_tag="VW528_001474"
     CDS             complement(9331..10260)
                     /locus_tag="VW528_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9912284.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10400..10687
                     /locus_tag="VW528_001475"
     CDS             10400..10687
                     /locus_tag="VW528_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9912285.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10721..12055)
                     /gene="ciaH"
                     /locus_tag="VW528_001476"
     CDS             complement(10721..12055)
                     /gene="ciaH"
                     /locus_tag="VW528_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9912286.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12045..12719)
                     /gene="ciaR"
                     /locus_tag="VW528_001477"
     CDS             complement(12045..12719)
                     /gene="ciaR"
                     /locus_tag="VW528_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9912287.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12828..15374)
                     /locus_tag="VW528_001478"
     CDS             complement(12828..15374)
                     /locus_tag="VW528_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9912288.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15497..15937)
                     /locus_tag="VW528_001479"
     CDS             complement(15497..15937)
                     /locus_tag="VW528_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9912289.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQAHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15912..16781)
                     /locus_tag="VW528_001480"
     CDS             complement(15912..16781)
                     /locus_tag="VW528_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9912290.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPSVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16823..17551)
                     /locus_tag="VW528_001481"
     CDS             complement(16823..17551)
                     /locus_tag="VW528_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9912291.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17559..18038)
                     /locus_tag="VW528_001482"
     CDS             complement(17559..18038)
                     /locus_tag="VW528_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9912292.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            complement(18049..18747)
                     /locus_tag="VW528_001483"
     CDS             complement(18049..18747)
                     /locus_tag="VW528_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9912293.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18740..19006)
                     /locus_tag="VW528_001484"
     CDS             complement(18740..19006)
                     /locus_tag="VW528_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9912294.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(19072..19998)
                     /locus_tag="VW528_001485"
     CDS             complement(19072..19998)
                     /locus_tag="VW528_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9912295.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(20073..20888)
                     /locus_tag="VW528_001486"
                     /pseudo
     CDS             complement(20073..20888)
                     /locus_tag="VW528_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20892..21212)
                     /locus_tag="VW528_001487"
     CDS             complement(20892..21212)
                     /locus_tag="VW528_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9912296.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6481 a         4692 c         3746 g         6482 t
ORIGIN      
        1 taaacaaaaa tgttttagag gcctatttac cgtggactaa agttgtacaa gaaaaatgca
       61 aataagaaat ctccagatta ggaactatcc gtgagttctc tagtctggag atttttcaat
      121 agacttcgtt attggacggt taccaagtta ctcaaagtac agatttgaag ctgcagataa
      181 gactgacgaa atcactttaa tactaaatga aaatcaaaaa gcaaactaag aagctagcct
      241 caggctgtac ttgagtacgg taaggcgacg ctgacgtgat ttgaagagat tttcgaagag
      301 tataaaacac tgttttgagg tggtggataa atcggctggg taaaaactca atctcaggag
      361 aaaatgaagt aaatctcccc acaactatag aaaaagattt gattctgctc ctcacacaat
      421 aaaatctttt tcttttatta aaaacgaatc gtttcacgtg ttttctcata tgaggtaaca
      481 aagcgattgg ttacaccagg ctctgccact tccaatgctt gagaaatctt atcaaatgaa
      541 gcgtgataat caaattcttt ttcaaaaata tctaaagctt cgttaaatgc ttcttgaatg
      601 cgttcatcaa atgagcgata gcggttagaa tattgcaaga gttgctctgt caaagttgca
      661 tattgtacaa tattataagt ttccgtttct aaagcttcca tatcattcgt tgcaatttca
      721 agaactcggg taacagattc aatgttaatc attttttgtt ctaactcaac cattaaatcc
      781 tcggtattat tgcttgccgt aaagaataac ttcaagaaag tttgtggaat acctggcaga
      841 ttgcgttttt ccatgtatcg cttgatagta tggagacgat tgacataaac attggccttt
      901 tgacgtgcat taatatcatc tttctcaatt tgtgtcaggc gctcactaac tgaaatttgc
      961 tcatcttcaa tatcttttag ttgagtttgt aaatcctcaa gattttcttc aagaactgaa
     1021 taagcttggg gtggttcttc ttgatttgaa gttacctcaa caatagctgc ctcaaaactc
     1081 tctaattctg tctgaatacg acgaacatgg cttgcagctg tctcaggaag taaataggtc
     1141 ttgttcaaac gtgcaatatc ttctcccaat aaagtattat tctctttcat atgttgaaga
     1201 taagttggaa gagttgcaag tagattttcc actactttct gagcagcaat ttctcgagta
     1261 aaaatatcat acaaggcatt gatttcctct tgtgcctgtc cattctcata ttcggcatta
     1321 tccaattcca actgacgaat attctcttgg tttttcttga atgcttcata aagcaagtgg
     1381 aaacgcgctt caatatccgt ttcaacaaaa tgataattag catcaattag tttacgataa
     1441 ccggcttcca aatcctgtaa ttgatctggc aattctgtag aaagcgtcgt aaccaaggct
     1501 ggaacacgat ccacaatatg acttaaggcc aaaatgtgat tttctgtatt atccaaaatc
     1561 actgcggctt ccacagggtc acccgatgaa ttcaaggtta caaattgtga aaattcagat
     1621 tggatatttt ctaattgttt ttcaatttca tccaaggctt gaccatactg ttctgaattt
     1681 tcagcaactc tatgctgaag ttcctcaaat aaatccaaag catgaagaac acgaccacta
     1741 tttttagatt cttgcttctc taagtctgcc aaagcattgc gaattgccgc aatatcttct
     1801 tcaatcaaag taatttgact ctcaatttgg tcaatttgat gactggcctt gagaaaacga
     1861 aatgaatggt tataaccttc tgcttcaaag agattatttt caatatcggc aaaagagttg
     1921 agagataaat cgacccattt ttgattccat tcacggaaag ccacttgact ttgtccaatc
     1981 aagtgcatat tttttacagc ttctacttca tcatttactg gaagattgta tagttcttct
     2041 tttctttctt ctagtgcctc taatctcccc tcgtttcgct tacgtagaaa gattgccact
     2101 acataagcca gaactaaaat gactgcaatt gcaaccatta aataaattag ttgtccatta
     2161 gacatatcaa actccttttt tacttgaaac aatcgtaacg attatatcat attttttaga
     2221 ccaagggaac tacttctccc tattttttag acatcaagtg tactatagac agcattttct
     2281 tcgataaact cacgacgagg ctctactcga tcccccatca acatatcaaa gattttatct
     2341 gcttctgcag catcatctac agaaactcta gccatcaagc gatgttcggg atccatggtt
     2401 gtttcccaca gctgatgatc gtccatttca cctagcccct tataacgctg aatagtcggt
     2461 ttggtacgac cttcactata acgggctaaa gcttcttgga gtttgatttc ttgatctgca
     2521 cccggctgga tatattcttt aatctcgctt ccaaccttga caccatagat tggtggttgg
     2581 gcaatataaa cataaccagc ttctaggatt ggtttcatat aacgataaat caaggttaaa
     2641 agaagggtac gaatatgggc tccatcgaca tcggcatcgg tcatcaaaac gagtttttgg
     2701 taacgggctt tcgaaacatc aaattctgcg ccaaatcctg ttcccatggc tgtgaaaaga
     2761 ctacgaattt cttcgttagc tagaatctta tccatacttg ctttttcaac gttcaaaatc
     2821 ttaccgcgaa ttggaaggat agcctgaaac tcacggttac gaccagattt ggctgatcca
     2881 ccagctgagt ctccttcgac gatgaagagt tctgtttcag cagggttatt agaagaacag
     2941 tctgctagtt tccctggaag gttggaaatt tccaaaccag attttttacg tgtgacttca
     3001 cgcgcacgct tggcagccac acgagccttg gcagctaaaa tccctttttc cacgatacgt
     3061 ttggcaatct gtggattttc catgaggaaa tcggagaagg cttcactgaa gaggcgattg
     3121 gtaatcttga ccacttcgct atttcccaat ttggtcttgg tttgtccttc aaactgtgga
     3181 tttgggtgtt taactgagat aactgcagtt aatccttcgc gaacatcttc ccctgttaaa
     3241 ttatcttcat tgtctttcag taacttattc ttacgagcat aatcattgat aacacgagtc
     3301 agtgccgtac ggaaaccttg ttcatgcgtt ccaccttcat gggtatgaat attattggcg
     3361 aaactcatga cattttcatg gtaacctgtt gtgtactgca tggctacttc aactgtaata
     3421 tcatccatct caccgtctgt atagattggt gtatcaaaga ttacatcctt gttctcgttg
     3481 atatattcaa cgtaactagc aatcccacct tcataatgat aatgcttggt ttgttccaaa
     3541 ccttggcgct tatcagtgat ggaaatttga agaccgcgat ttagaaaggc caactcttga
     3601 atccgtttat ttaatttatc aaaatcaaag attgttgttt cagtgaaaat ttctgggtcc
     3661 ggtgtgaagt gaaccgttgt tccagttcta tctgtatccc caaccacctc aaggtctgca
     3721 acaacatgac cacgacggta ttcttggtaa tgaatcttac cgtttttatg gacataaacg
     3781 tctaattgag tggaaagggc attaactact gacgacccca ccccgtgaag accacctgaa
     3841 accttgtatc caccaccgcc gaactttcct ccagcatgaa ggactgtaaa gacggtctca
     3901 acagcaggac ggcctgtttt ttcctgaata tcgactggga taccacgccc atcatccaca
     3961 acagtaatcg aatcatctgg ctcaataaaa acttgaatat ggctggcaaa tcctgccaag
     4021 gcctcgtcaa ttgagttatc aacaatttcc cagactagat ggtgaagacc ttcttttgag
     4081 gttgatccaa tgtacatccc tggacgcata cgaacagcct ctaagccctc taaaacttga
     4141 atttgactgg catcataatc ctgtgcctgc agatttttga tttcttctgt catcttattc
     4201 ctttttctta tatgtctaat acttgtctta aattcacgat actggtaaac aagtgggtat
     4261 caagtcctgc agcttgacct gcttcgatat caatcggccg atcaccaatg acaagaccag
     4321 agctaatctg atacttttct cttaaataaa gcatggattc gggatttggc tttctcttaa
     4381 agcctgagct agaagtcacc acttctgtaa aataagctgc tatagaggtt ttttctaaaa
     4441 tttccaaaac ctgatcattt cgatgagaga ccaaaaaatg acggccacct tgatttgaaa
     4501 tgacttccaa taggtcagaa actccttcaa ataaaatcgg gtgttcaagc tctctggctt
     4561 cattttcctt gtacttttct aaaaaattct ctaaattggg agcgaatgtc tcaatcgcaa
     4621 aaggagtaga aacctttaaa gcttgataga cactgtcatg gtcttgtgtg ataccataca
     4681 gtgccaatgt ttcaacaaat gcagctgttg aagtttcata attatccagt aaagttccac
     4741 ctaaatccca gatgtaatcg tgatatttca tgccctttat tatagcattt ttttatgaaa
     4801 aaagcgatga tttccctgag ttttccacat aaaaaacgag agaataactc ccgttttact
     4861 gatttttacc aaagacatct tgatagagca ttcctgttcg taaactctct gcgtcccctc
     4921 ctagttgctc caccaactcg taggcaaagg caagggctgt tgaaggaccc cgactggttg
     4981 tcaactgacc atctaccact actgtttcct tgacgtagtg accatcaagg atttgctctt
     5041 gaacgccgtc ataacaagtg tatcgcttat ttttcaatat ctctgcttga ttgagggcaa
     5101 ttggtgccgc acaaatggct gctagtttct tcccttcttg ctcgaagctt tgcaattctt
     5161 gaatcaaggt ctgattatca cgtaaatgtg cagaaccagg catacctcca ggaagaacaa
     5221 tcatatcata gtctgataaa tctccatcaa agacatgatc tgctcttact tggattgcat
     5281 gcgaacccgt tacttgctct tcaaaaccaa ccatatcaca tgtgatattg gctcgacgca
     5341 agacatctac aactgtcaag gcttcaattt cttcaaagcc ctgagctaac ataactgcta
     5401 cttttaccat gattttctcc ttatttaatt tgttttaata caaccttttt ccgtttgaat
     5461 gggacttttc cgctcttttc ttcagctaaa ttagtctggt aactcatcga taaaagcaaa
     5521 ccaacaccaa tcaaattact gataatagct gatccccctt gcgaaatgaa aggcaaggga
     5581 atccccgtca aaggaagtag tccagtcaca gcaccgatat tctcaaagat gtggaagagc
     5641 aacatcataa tcaaacctgt ggaaatataa gtgtagaact ggttatttga tttaagagta
     5701 atcttcaaca tacggtaaat caacatgaga tagagggcaa taaccaggac agagccaata
     5761 aagccaaaat cttctgcaat aaccgtaaaa atcatatctg actctcgaac tgggataagc
     5821 agattcgaag cattaaatcc ctgaccaaat aagccaccac tcccaatggc aatctgccct
     5881 tgagcctgct ggtaagtcgt tgtttgggca aactcaaagg gattgagcca agccaaaatc
     5941 cgattaattt ggtaggtcgg cattccaatc tggtgaagaa aagctcgtcc gtccttgcta
     6001 ataaagatag ctaagaaacc agcaactcct gttacagcag tcacaaatac tgggataata
     6061 attttccaag aaacccctga taataaaacg attcctgaga aaatggctac aaaaaccaaa
     6121 gccgtcccca agtcactttg aagtgctaaa agaactagga ctggaatggt aaagagaatc
     6181 atccagaaaa ttaacaaaaa gtccagcgga accgtgcgtc tccattcctt atgtttcttt
     6241 gtaaattgga caatgacacg agccaacatg aggatatagg atatcttcat aaattctgac
     6301 ggttggaata gggtaattcc atttattgat acccagtttt tggcacccgt tgatgcaact
     6361 aagcttggat tataaaatac aatcggcaag atcataagtc ccaagcctaa aatatataga
     6421 aagggggtca ccttccaaag aaattctgta ttaaagagca tgaccacaaa accaatcaca
     6481 agccccaagg caatccaggc gacctgctgc cctaaaatgg gcagaatatt attgggataa
     6541 tcatgactaa cggctatata gatagccacc acaccgatga ccagtagaaa aaatactggc
     6601 aagagcaaac tgtaatcgac tcttgagtcg agagaacgtt tcatatatac taaccttata
     6661 ctttcataca atactattta tcaaagttca ttaaaaaatc tatcaatagc ctcgtcaact
     6721 tcggatcgag agatggtttt aacagtcgct tcttctgcta gagatgctac tatttgtttg
     6781 ccgtatcgtt ttccgacgat tctcctatcc aaaataagag ttaaggaacg ttggtattca
     6841 cgtctcatac ttcttcccaa agcctgtttt aaacgaataa tggccattgg caattgataa
     6901 tcataaaagg catttttccc ttcttgattc agttcttgat taatcttttt cgtcaagggt
     6961 tcttgaggat tttggaaagg aagcctcggt acaacttgaa tcacaaaagg atggcttgaa
     7021 aaatcaactc cctcccagaa acttgctgca ccaagcaaga tttgttgttc acctttttca
     7081 aagcgtttct ttagctgatg aacatcccca tttttatact gggccaagtg gctaactgta
     7141 agtaaatccg atactgctag aagcatgtct ttagcggtaa agagaaccaa aatcggttgc
     7201 tggaaagctt gaatttccac tagtaaagca gctacctctc tggcatagac ttctaaggag
     7261 gtttctgtta ccagagggaa atctttgacc ataaccactt cttgttcctg ttttccccga
     7321 gattcaatct tgacaaattt ggcttcagga tagcctaaaa ggtctgccaa agaaaccctc
     7381 tgactaatct caagagtagc cgatactccc aagacttggc aactctcagg aagtaaagag
     7441 gacagaagag tacggcctga ttttgtagaa gaaatctgaa ttttcttttg actcgtttca
     7501 gttacttcaa gccagtaatc accttcagct gtaaaatagc gaaccagctc atcaaagcct
     7561 tctacttcta attctgaaaa atactgatgg agattgtcca gagaatctaa gatatttttc
     7621 ctagatttac cagactgaaa ttgttctatc aagtagagac actcaaagcg aatactttct
     7681 agtatccgtt gttgaaccct gttttcttct cctactaaag ccttatcaat taaatcgata
     7741 atagattgta tatcgtaggt ctcttgaagc agattttcta gagctaacaa aatcttttgg
     7801 acttcatcaa taatcagtaa acggtcactg acaaattcag gattatcttc aagtctggtc
     7861 acgagatagg catgattagt cactaaaagc ttgcaagtct ctgccctttc ttgactacgt
     7921 ttccaaaaat cttccgtcac aaataagctc tgggatgata aattcccatc atgacgaagg
     7981 tctgctagaa aatgttggta acggtagagt tgcccgattt catccaaatc tcctgtctct
     8041 gtctcagtaa gccagaccaa gacttgcatt ttaaagcgtc taaataagcg attttcatca
     8101 ttttcctgca aggaatgata aaaggcatcc aacttcagat aattttgtgg tccctttaag
     8161 ctatgaatat ctgtatggaa cacttccttg agacgtttac cttcttcttc catgatttga
     8221 ttttgaagaa tctttgtcgg aacactaaga acaatttgtc gctctttgga ttgagataaa
     8281 gcgggtaaga gatagccata ggttttccca atccctgtcg gcgcttgaat cagagagaca
     8341 ggttcatctt tcaatagcaa gacaacctct ttagcaaaac tttcttgttc ctccctcact
     8401 tcaaggttca acagagaaat atttttagaa aagtcttgag atagttttcg tagctccaga
     8461 gaagctgccg ttttcttaaa atacagacct tgaacttgga ccaagtctgg agaactcagg
     8521 atagattggt tgcgataagt ttcctcaata accaggtagg actcatatag gagagcgtca
     8581 gccatttcca gcaagcgttc caagagacct ttaggaagct gggtcatctt ttcccgtaaa
     8641 aaaagaagta attctgctgt agcttgggca tctgaaaggg ctgtgtgtgc gtgtttaaga
     8701 ggaattccta attctcgaca caaaatcggc aagctatatt tttccagttc agggaaaaag
     8761 acctgggcca attcgaccgt atcaacacga gggtttctta gctcatagcc ttcaaaaaat
     8821 aaattttccg ccaagagatt agcatcaaac tgaacattat gggctacaaa aatcccatcc
     8881 tccaccaagt caaatatttt tctggcaact tgcgaaaaat caggtgcttg cgccagacgt
     8941 tggtctgtca atcctgtcag ttctttgata tgagcatcca agggttcatg tggattgaca
     9001 tccgtcgtat agtgatcgac gatttctccg tcctcaatca cgacaattcc cacttggata
     9061 attttagcct tactacctgt gctagttgcc tctaaatcca ccacaacata gcgattacca
     9121 atcgttttca ttataaaaaa ttataccaaa aaaagggcta tttggcacct gcaaacatag
     9181 gataattttc aaactcaaga acactacttt tcttaaaaat cccatcgaat agagcattct
     9241 agcctgctga tttttgtaaa atagtgaaga aatacgaaaa attgagagaa gaaatgtttt
     9301 catcttcctt ctctcaacga atcatgtatt ttatttaacc atgtcaggat cgtagtcata
     9361 aaatcctgta tcaaggaaac ggtttttagg gtgaagttta cgaacttctt catccagcaa
     9421 gaaggcttga tcatggctaa agttgccctc ttgaatcaag ttacgcgctt ggataaagag
     9481 ttttgcaatc tcaacaaagt tctgaccagg agtgtagagc ccttctagtt tccagcgagt
     9541 aaagccatgc tccaccaatt ctgttaattt gatcatcaaa tcaaggtcat tgttggcaaa
     9601 gatatgggtc ccatgattat cttcaaaaat ggaatagtgg ctctctggat cacttggctc
     9661 agccaagaag aggtcacgtt tatgcgtctt ttcatcatcg atatgtgtaa agttatagta
     9721 gttttgcaag agtggacgtt tagaatgatg gatgacgcta gcaccgtaaa ccaaaacttc
     9781 agcaggaatt tccaaaatct ctggcatttt gaaaagttca gctgatggaa tttcacgcgc
     9841 caaaacagcc tcagatgcac cagccttttg tccccagaag ttaatctgac gactgctagt
     9901 taccatggtt gaagcatcgt agatggtctt aaatgaataa ccatcgcggt taactacgta
     9961 aaagacgcct gcatccccaa tcgtaatata gtctgtcttg atttcttcca agaagtctaa
    10021 gaaaggcttg atacggtcca tcatatcttg gtgcatgaga gcattgaccg caacgagcaa
    10081 ttccttacca gcatcatgaa ccaacttagc gatttcacgt aattggtcat aactaaaggt
    10141 cgttggcaga cgaaggccaa aatctttctc accgacatag atacggtcta cgccagcttc
    10201 gagtagttgt tcaacttgtt caatactttc agcagttgct gtaatgacaa tcttttccat
    10261 aagaaaaatt ataccacatt tctccaaaat cagccatgct ttgaaaatga aaagggactt
    10321 tattcttttt gtaacctttc tgtaataatt ctctgccgaa aaaatgataa aataggtatg
    10381 tactgttaag gagagaatca tgcccgtaag aaaattacaa tcctatgagg tagactatca
    10441 agaagaatta aaccagcagc ttcctcatta ccaagcttat acacctgaag cgcaatctga
    10501 tgccaatctc aaggaaatcc tattttttat taatatcgct gttttttgta tctgtattgc
    10561 tatctttagt tttatctttt tagcattaaa attatcaact gctcttgcct ttgccgcagc
    10621 aatcggattc agcttacttg ttttaaaagt tcaacggtct attatcaaac gaaaacgtag
    10681 aagataaatc caaaatatcg ccccaattgg agcgatattt ttattttttc tttttagatg
    10741 gtgtctgaat ggcaatcttc acttcaaaga ttgttccctt gggtttatta tctttgacag
    10801 taacagttcc ttttagagca tctacaattt gcttggctag ggataatcct aaaccaaaac
    10861 cacctttttg ccgggttcta gccttgtcta ctcgataaaa acggtcaaaa attttctttt
    10921 tatcttctgt cgaaataccg attccattat cagaaacaag taaataaaga ttgcgatcgg
    10981 tcgccgagat aagaaaatca atttcaccat cctcctcagt atacttgacg gcattatcga
    11041 aaagaatggt catcagttgt ttcagaagaa gctgatctgt gacaattgtt cgatggatac
    11101 gattttcaaa acggaagaca cgattatttt ccgaagcaat catctcatag tttgtgaaag
    11161 ttgtattaaa aaagctagtt ggaacttctg caagctccgg cttaatccca tcatctctcc
    11221 gagctaagtt cagcaagctt gtcgttaaaa aacgcatatt tcggacttct tccaaactcg
    11281 atgcaatgct ttcgctcaca tccataatgg tagcttctgg cttacgaaaa agggtctcta
    11341 agcgattttg caaaactgcg agtggagttc gtaactcatg actggcattt tccacaaaag
    11401 actgttgctt ctgcatactc tcaagcaagg gcctaacact gaccctagct agatagagac
    11461 tggcaagtaa agacaaaatc cagaaactag ccatcacgac cacaatcaat tgctcatgct
    11521 tttgactagc ctgctccaac tgactggtat taatcaagac agcagcatac ttgatattgg
    11581 ttgaaaccga actaatattg gtctccatca aaatcacacg atagatttct tcctgcccat
    11641 agctattaaa aacctgaatc tggtagatat gtcctagttc tttcttttct aacttaatct
    11701 tatccaagcc caaaaatcga tttccagaaa gaagttgagt aaagtcttta tcaaagagaa
    11761 tgacttccgt attggaactg acattaggtt ttatttctac tttactagcg tccgccctag
    11821 cattttctaa atctttaatc tcttctgttg ccctatttat agccagctga ataactgctt
    11881 gaggattttc actcagtcca tgaagcttat cgtccaccga agtataaaga ctcgaatgca
    11941 tgacttgtaa aataatcaga gtcattgtag aaaaaatcag ggtgaagaca ccgaagttgc
    12001 ggataaaata actaaagtca tccgcatacc atgttttttt aagtttactg aacatctttt
    12061 aaaagatacc caacactacg caaggtttgc aaattctctg caaaagtggt tccctttaat
    12121 ttcttacgga cttttgaaac atagacttcg acaaccgaaa tcgttgtatc actatcaaat
    12181 ccccatagac ggtcaaaaat ctgcgtctta ggcaaaatca cattttgatt ttgaaggaaa
    12241 taaactagta aatcgaactc tttccccagc aattcgacag gagtatcttc aactttaacg
    12301 gtattggttg ataaattaac cacgatattc ccataagtca aggtgttttc attaaacttc
    12361 cctgaacgtt tgagaagggc ctgaatccgc attttaagtt cttctaggta gaaaggtttg
    12421 gtcagataat catccgctcc cagttcaaat ccatgtccct tgtcatccaa actttccttg
    12481 gcagtcataa tcagaactgg tgtcgtaatt cccttttcac gcaattcttt taagacttgg
    12541 aaaccatttt tttctggcaa catcaaatcc agcaaaatca agtcatagac accactctca
    12601 gcttcgtaga gaccttcttc tccatcaaat acctgcataa catccgcaaa atcgtctaaa
    12661 aagtcaaata ctgaatttga cagacctagg tcatcctcaa ccaataagat ttttatcatg
    12721 agaaactcct ccttattaaa actattatac caaatttgcc ttaaaaaaaa ctcaactctc
    12781 tgcattttac atgagatagc tgagttttct ttttatttta ggcttattta tgcatttccg
    12841 tattgaagaa caactgcttc gactgcagct ttttcacggc taatcaagtc aacacgtgct
    12901 gcaatttcct tgattcccat accgatgtta cggctaagag caaggtcaga aagttgcggc
    12961 tcaaagaact ccttgtattc cgccaagcgt tgctgagtct taaatacatg agcaggaagg
    13021 ataacaaagc tatcaaagct catatctcct ccaagggctg ccttaatcca agcccagttt
    13081 tcacgcgccc aagaccaagc tgttttctga gttgcttgat gagctaggaa ttggtaatac
    13141 caagcagaca agtcctgtgg tttgaccaca aatttgtcct tccaagaagt aatcaagttt
    13201 tggatattat ccgcatctgt actgtatgca agagctgctg ccaactggcg tttaaagaca
    13261 gcatctgttg cgtgagtata agtatcaaga taaagtgcta acaagtcttt agtctcatga
    13321 tgtttcatct cattaatcag aacttgtgaa cgaatagctg ctgggagtcc tgcaagattc
    13381 tccttgtgtg ttgcgaagat ttggctagcg acttgactag cttctgcatc atttgagcga
    13441 atcatcatcg aaacagccag ctgacgaacc aattcatcct catctgattc tccgtcttta
    13501 gcttcaaaac caagacggtc atagttatga cgagccaatt tagcaaccag tcctttgaag
    13561 gctgtttcag catccgttcc ttcatcaata aagcgctcaa gggctgaaat cacttgagaa
    13621 acagctgaaa ccaccagata agactcttcc ttagcaagtt tatcaagaac tggaagcaag
    13681 tctgcataag aaatgtgccc tgcctcagcc aacaaacgac gttcttgaac aatttgcagt
    13741 ttgcttgtgt tatcaagtgt ctctagctca gcaagaacag cttctaacaa gtctccttga
    13801 tagtcggtaa tatagtgggc agtattttca gtgttgagac gaagagctcc ttcattttca
    13861 gcaagaagag ctgcgtagcc agggatttcg atactttcag tttcgagtgt atcaggcaag
    13921 cctttccagt tgctattgag gggcaccacc cagagacggt tcttgtcttc gttctcaccg
    13981 atgaagaatt gtttttgtga aatcttcaag acatcatttt caactttaac agtaagaact
    14041 gggtaaccag gctgttccaa ccaagaatcc atgaaggctg cgacatcacg tcctgacgct
    14101 tgaccaaggg catcccaaag gtcactacca atggtgttgc tgtattggtg tttttcaaag
    14161 taggcgtgca aacctttagc aaaatcagca tctcctagcc aacggcgaag catgtgcatg
    14221 agacggcttc ctttggcata gacgatagcg ccgtcaaaga gtgtattgat ttcatctgga
    14281 tgtttaactt cgacgtggac agactgaacg ccatcagtag cgtcacgttc aagagcaaga
    14341 ggtactccac ctgtttggaa atcttcaaag atattccagc ttggttcgat ggtatccaca
    14401 cagacgtatt ccatcatatt agcaaaactt tcattgagcc aaaggtcatc ccaccatttc
    14461 atagtcacga ggttcccaaa ccattggtga gccaattcat gggccacaac aagggcaact
    14521 tgttgacggc tagcaaatgt agagttctca tcgacaacca agtaaacttc acggtaggtc
    14581 acaagacccc agttttccat agcaccagct gagaagtcag gaagggcgat gtggagagat
    14641 tgaggaattg ggtacttaac tccatagtaa tcttcgtaaa actcgataga gcgaacagcg
    14701 atatccagtg agaaatcaag atttgaaagt ggatgtgctt tggttgagta gacacctacc
    14761 agggtaccat ttttagtttt agcggtcacc ccttgcaaat caccagcaac aaaggccaac
    14821 aagtaagaag acatgcgagg tgttgtctca aacttccaga tacctgtttc cttacggttt
    14881 tcaacatcga tttctggcat gtttgacaag gccaattcac cttctgcttg gtcaaagcga
    14941 agagagagat caaaagttgc tttggcttca ggctcatcca cacatgggaa agcttcgcgc
    15001 gcaaaatggc tctcgaactg agtagacaag acctccttct tgactccatc aactgtataa
    15061 taagaagggt aaatccctgt catgttgtct gtaattttac cagaaaaggc aagaaccaat
    15121 tcaacttgac cagcctcagc caattcgata tgaagggctt cattgtcatg gtcaactgta
    15181 aatggacgag cttgacctgc aacttctaca gaggtgattt ccaagtcttt ttggtggagg
    15241 gagatgcggt cactctgtgc ttgaccagtg atggtcactt tcccagaaaa agtcttggtc
    15301 tcacgactca aatctaaaaa taaatcataa tgttcaggaa caaattgctt aataaaatgt
    15361 tcaactgctt gcatagtttt ctcctattct aagtttaaga gctgctgctc ttcttcaaac
    15421 aaacttatta tatcatattt tgcttgagaa aaaaggattg gacagcgatt tccaaaactt
    15481 tcttccttct aatagtctac agtgggtaga ccttgcgaaa ttcttctaaa acaaccttgc
    15541 tttcaggtgt aaaagtcagc cctacttcac ccaaacagtc tgtgaaaaag tcctcatgag
    15601 cctggcgcca ataggcaagt gatttgtctc cctcaccttc cttaaaggca tgttgcgcag
    15661 agacttgatt gaagagttca acagaaacct ttgtaatttc gacaatgcag acagactgat
    15721 tttgactatc taaaatgaca tcgaaggtcc ctacttgggg aagtggttcg tcttctagta
    15781 catagaggtc ataggctgat gctgttgctg tcttttctcc tttaaacacc aaatccgcta
    15841 aaaggtctgg ttcaactcca aaagcccaag catcgatttc atctccgatc aaaggattga
    15901 tttgcttgta tttattccac atttcttgcg gtatcatggg tactcctttg taatttttta
    15961 ctttcttctt ttatgtgttt aagatgatct ggatggtcaa tctctaaatc aaaaatctct
    16021 ggaatagaac tgtagtggat aatgcacttg atacccaact gattcatttt ttgtatgaaa
    16081 gaagtattca gatagcctgc tacagcaaaa ttaatcttgt tctttcttgc tttatcctgc
    16141 atatctctta gcatatctaa cattattgga ctttccatat catgccattg actgtttctc
    16201 atagtcgcaa aaacaaagga agtcaaatca ttcattccaa ctacaatctt tgaaatgccc
    16261 gtttccagta tactagataa gtcaaaatac gctgacggta attcaatcat cgttccgact
    16321 ttcccagtaa agccatactg acgcaatact gtaatagctt gttttaattg gtcggcatca
    16381 ttgacagaag gaaagataag agatagattg gggttggttt gataaacttc tgtaacgaca
    16441 tgtgcttcag cctgaaattc atccaaacac gccagtaaac gtctggttcc tctatagcca
    16501 aacaagggat gcccttcgtc aaaaaactct ctagtcccca ctaaacaatt ggcttctgta
    16561 ttcgttaatt cagaaaaacg ataccaaact tcttcatctg agtacaggga gcagatagtc
    16621 tctagataat cttttacaaa ttgctgacaa cttgctaata tgatgttttg attaagctct
    16681 ctcaacaaat attccccacg aatcatgccg acgtggtgaa atagttgagg ataaattttt
    16741 tcaagaattt tttcgccact aagggcaagt tgatttctca tagccattca cctcgtctta
    16801 aaagatacaa tatcatatat aattatatca aaatttcctc tagttcaaaa gcttcataag
    16861 gttcagataa ctcatatcct aaaaattctg ctactttttt ggaagcctca ttatgagcat
    16921 cccaaagtgg aaacatatct ctatttaaac tctctagaat cattgcagca ccaagctttt
    16981 tagcaaatcc atttccttgt tcgtttggtc tagttgcaac ttccacttca actgctttac
    17041 ggtaaactaa ccctgaggaa atcccagcaa tcagttcatt atttttaaga atcacaaagc
    17101 caaatccacc ttttaaaaca aaatcctgat aggactcaaa atccccctgt aaatcctgtg
    17161 accattcttc cgtagaaaag cagttataaa tatgattatc aataggcaca atattgtaac
    17221 cttcctctaa atgagtaact agattattta aaaattcaac ttgaaaattt gccttatctt
    17281 taaaagaata gcgagtaaaa gaatttagcc ccacttttcg atgcagaaaa tcttgccaat
    17341 tagtattttc tgaaataata atcttataat ctaaaccata ttttctaaca aaatcttccc
    17401 aaaatctgga atccaactcc cctgctaggt atagaaaatt cccaacatca taaaagcttg
    17461 ttgtcctatc attgtttcgg tagacagttc caatttgaga tttaagacca tagagaacca
    17521 tatttttctt ccatgtttta aatagttcca ttatccctct acttcacctt ccaattcatg
    17581 taagaagtct tgtccagttc tggcaatcct aataattcag acttaacctt caagactaat
    17641 ggcgatgcat tttcttctgt aatctcttga atatccatcc aaatatatcc aagtgaatca
    17701 ttcgcaccat cagacacagc ttccgaaatc gtaacttgag gtgcactctc attcatttca
    17761 acatcataca aggctatgac atggtgaacc ataaaatttt ttaactcttc cctgacgaaa
    17821 acatcgtaga ttcgaggatt agagtagctt ctaacagtaa atcccgtctc ttccataact
    17881 tctctagtca gcgtttccgt cagtccttca ccaagttgct gactgcctcc aggtagatca
    17941 taccgatgtt gataagggcc tctcgttttt tcaatgcaaa gtaactttcc attttcaaag
    18001 caaacacagt agaccccaaa gtgatttttg atttccatcc aactcctcct acttcaaaga
    18061 ccagccacca tctattgtca gaatttgtcc ctgcatggca gatgcctttc cactggccaa
    18121 aaagagactg acttctgcca cttcctctgg ttcaatccag cgcttgattg gtgtttcact
    18181 agccacccag tcagctaagc cacctgtctc aaaatccgca gcggtcatag ctgtcttgac
    18241 tgctcctgga gcgataccaa agacctgaat cccagcttca gcatagtcta gagccaactg
    18301 cttggtgaag ccagccaagg catgcttgga tgaagtatag gcgtgaccac ctccacctgc
    18361 taggctagaa gcaatggaac acatattgat gatgattccc tttttatttt ccagcatttg
    18421 tgtcaaataa taccgagtca actctactgg aataatgtag ttgatttcaa aaatctcttg
    18481 aatgtcctgc gccgtttgtt ccaacagtgg tttgtaatca tccaaaactc cggcagtatt
    18541 acacaaaaca tccacctgag ggcaccagtc aaaaataggt tccaagtcca aggtcaaatc
    18601 tctctgtaaa aagtgaaaat caccctctaa gagtggcttt tcaccttggt caactccata
    18661 aacttgatag cccttctcta aaaagaggcg agcttgagcc aatccgatcc ctgaactcac
    18721 tcctgtaatg agtacacgtt tagtcatgca cttctaccca atccgttgcc aaaacatcac
    18781 aaactgtcgg gctccacatg gaaaaacctt ctccttctcc agaaacgttg attaggaaat
    18841 aaggtgtcat ttcaagtgca agcccatttt gctcgatggt atcaaagagt tggacatagt
    18901 tttccgcacc tccccaacca gttcgtacat attttctctt agcctttaac ccaggcagga
    18961 tctcttcaaa tgtcatgttt ttctccttta attctacatt cttcatttaa ttatagcaaa
    19021 aaaccacttt atacggcttt ttgactgtga gttattcaaa cctgctacta cttacggcaa
    19081 attattccct gcagcaagat aaatttcata ccattctttt cttgttaagc taaagtttgc
    19141 cgctcggcta acttctctca agtgcttagg atttgttgta cctacgactg cctgcatttt
    19201 tgctggataa cgcaatatcc aagaaatggc aatagttgaa gaggttactc catatttaat
    19261 agctaaacga tcaagtactt gatttaaagc ttgaaatttc tcatttccaa caaagttccc
    19321 tttaaaatac ccgaattgca agacagacca tgcttgaatg accacatcgt gtaatttgca
    19381 atattcaaaa atgctgccat ctcgcatagc tgcttgacta tcttccatat taacatgaaa
    19441 agctgattca aatcctggag taaaagccgc actcaattgt agctgattaa cagctaacgg
    19501 ctgcttgaca tcttttttaa gcaactccat catcatagga ttttgattag aaactccaaa
    19561 atctcgaact ttaccttgtt tataaaggag atcaaaggct tctgctactt ggtcagattc
    19621 catcaaagca tctggtcgat gaaggagcaa gctatctaga tgatcaatct tcaatctttg
    19681 caaaataccg tctactgatt ttataatata gtccttagaa aaatcaaaat aggtaaattc
    19741 ttcaatgcga atgccacatt tggactgaat ccacatcttt tctcttaaat ctggacgatt
    19801 ttttaggaca agacctaaca gttcttcaca acgaccacga ccataaatat cagccaagtc
    19861 gaaggcattg attccaacag aaagtgctgt ttctacaagc tcttcaactt cttttacaga
    19921 cttatctttt attctcatca ttccgagaac aatttctgat aattctttgt catcttgacc
    19981 aagagttatg tatctcatca aatttttctc ctttaatttc taacattctt cccttcatta
    20041 taacaaaaaa ccgctttgca acggcttttt gactatactt cactccattt tatcttctta
    20101 aacccacgga acaagacaaa gattccaata aagaggacag ctaaaggaat aacttttgta
    20161 aggaaaacat ttgaaattcc catctactca taataacgga gcagagaacc caccacaaga
    20221 tgggcaataa tcatactgac aaatggacga aagaccgctt ctttccaatt ccaaataccg
    20281 ataactagcg aaatcgtaaa gacagacaaa ctatcccagg gagccggaat ataaaaggct
    20341 ccttcttgta tgaagcttgc cattcctaca tatcctaaaa caactagaag aactatagtc
    20401 ccaacaacaa tgtaagtgcc aattttcatt ttaggagaat cttggactaa acttcttcgt
    20461 aaaattgtgg ccacaagtcc aaatccaatc agaaaaataa gaagttgccc taaaaatgtg
    20521 agcaaattga ctgttaagag aggaccttta gaaaaatcac ttagtagttg ataataacgt
    20581 aataccgcca ggacaagaat tggcgtcaaa agggactctt tgatagaact gcgaggtgct
    20641 cccttgagaa tctctttcat tattttttta ggattcttac ctagataatc ctctgcactc
    20701 atgccatctc gttctgcttc tgagaaatct agcatcatca aatagatctg ctctctgaga
    20761 tagtcttcat catagagaaa tccagcaaga ttaaaacttt cccacaactc ctcaaaatac
    20821 ttttgattct cctcagaaaa ctcatgtagc aaagcgcttg tttcttcgta atacttcatt
    20881 ttcttcatgg tttaaccccc attcttaatc ccttctactt tttgactcaa atcgtcccat
    20941 tgttgccaaa agactgagac acgctcttct ccttctttca ttaatgaaaa atacttccga
    21001 tctggaccat ctggcgacgg gcgcatgtcg cctcttatcc attgattttt ttctaacttt
    21061 tgcaacaaag gataaatagt tcctggaacg atagtatcaa atccagcctc tcgcaaagtc
    21121 tgaaccaact cataaccata ccgctctttt tgaccaatca tatccaagac acaaccttca
    21181 agaacacctt ttaatagctg agtttctttc atcacttctc ccttctaatc tattttgtaa
    21241 tacctactag taacttcacc tatagtatat cacttctaca ctagtttgta aagcataata
    21301 gttaatactc ttcgaaaatc tcttcaaacc acgtcagcgt cgccctaccg tatgtatggt
    21361 tactgacttc gtcagtttca tctacaacct caaaacagtg t
//
LOCUS       DAVKQB010000039        21208 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKQB010000039 DAVKQB010000000
VERSION     DAVKQB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21208
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.039"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..930
                     /locus_tag="VW528_001488"
     CDS             <1..930
                     /locus_tag="VW528_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU9912297.1"
                     /translation="PTDYDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVPIGFSN
                     KLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHSTA
                     NIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDFFAERRSLNAEVVIKGVCLDPRI
                     GNFYNNPSFEFGGYCLPKDTKQLKKEFIEINAPVIEAIDISNTNRKQFIVKQILERKP
                     KIVGIYKLGMKYNSDNYKESAILSIINELLIVGIKILVYEPNLNVSIDNVIFEKNFEL
                     FTKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            1325..2188
                     /locus_tag="VW528_001489"
     CDS             1325..2188
                     /locus_tag="VW528_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9912298.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFLYIPIFE
                     AELESMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFTQSLQVLENPQLTSLFE
                     LRLQQYKKLID"
     gene            2474..2671
                     /locus_tag="VW528_001490"
     CDS             2474..2671
                     /locus_tag="VW528_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU9912299.1"
                     /translation="MLNLQFAETMELTEAELETGYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            2739..3404
                     /locus_tag="VW528_001491"
     CDS             2739..3404
                     /locus_tag="VW528_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9912300.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            3476..4702
                     /locus_tag="VW528_001492"
     CDS             3476..4702
                     /locus_tag="VW528_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9912301.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLT
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(5052..5129)
                     /locus_tag="VW528_001493"
                     /pseudo
     CDS             complement(5052..5129)
                     /locus_tag="VW528_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            5200..5856
                     /locus_tag="VW528_001494"
     CDS             5200..5856
                     /locus_tag="VW528_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU9912302.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTLVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            5846..6169
                     /locus_tag="VW528_001495"
     CDS             5846..6169
                     /locus_tag="VW528_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9912303.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKHKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            6272..7102
                     /locus_tag="VW528_001496"
     CDS             6272..7102
                     /locus_tag="VW528_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9912304.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYNVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            7256..8110
                     /locus_tag="VW528_001497"
     CDS             7256..8110
                     /locus_tag="VW528_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9912305.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            8210..9583
                     /locus_tag="VW528_001498"
     CDS             8210..9583
                     /locus_tag="VW528_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9912306.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            9576..10637
                     /locus_tag="VW528_001499"
     CDS             9576..10637
                     /locus_tag="VW528_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9912307.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            10639..11331
                     /locus_tag="VW528_001500"
     CDS             10639..11331
                     /locus_tag="VW528_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9912308.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(11360..11929)
                     /locus_tag="VW528_001501"
     CDS             complement(11360..11929)
                     /locus_tag="VW528_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9912309.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            12064..12678
                     /locus_tag="VW528_001502"
     CDS             12064..12678
                     /locus_tag="VW528_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9912310.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            12680..14326
                     /locus_tag="VW528_001503"
     CDS             12680..14326
                     /locus_tag="VW528_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9912311.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            14338..15624
                     /locus_tag="VW528_001504"
     CDS             14338..15624
                     /locus_tag="VW528_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9912312.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            15676..16209
                     /locus_tag="VW528_001505"
     CDS             15676..16209
                     /locus_tag="VW528_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9912313.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            16287..16757
                     /gene="nrdI"
                     /locus_tag="VW528_001506"
     CDS             16287..16757
                     /gene="nrdI"
                     /locus_tag="VW528_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9912314.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(17050..18312)
                     /locus_tag="VW528_001507"
     CDS             complement(17050..18312)
                     /locus_tag="VW528_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9912315.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIIIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(18622..19080)
                     /locus_tag="VW528_001508"
     CDS             complement(18622..19080)
                     /locus_tag="VW528_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9912316.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(19077..19517)
                     /locus_tag="VW528_001509"
     CDS             complement(19077..19517)
                     /locus_tag="VW528_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9912317.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            19950..20816
                     /locus_tag="VW528_001510"
     CDS             19950..20816
                     /locus_tag="VW528_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9912318.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLNALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
BASE COUNT         6345 a         3759 c         4619 g         6485 t
ORIGIN      
        1 ccgacagatt atgatgagaa aattggtagt tttaatacat atgaaatcga acaaacggta
       61 tcgaagattc tgagggtaaa acctaatgga aagattattt taaagtcaac agttccgatc
      121 ggcttttcaa acaaattaaa aaggctgttt gatacaaaaa atatcatttt tgtccctgaa
      181 tttttgagag aaggttgttc tatatatgat aatttatatc caagtcgcat agttgttgga
      241 gatgagacag ttgaaggaag aaaaattgca gagttgttcc tttcgattag tactcatagt
      301 actgccaata ttaaaaatgt tatgttagtt tctcctactg aagcagaagc aattaagctt
      361 ttttctaaca cattcttagc tctccgtgtt gcttttttta atgaactaga tttttttgct
      421 gagaggagaa gtttaaatgc tgaagttgta ataaaaggtg tttgtttaga tccaagaatt
      481 ggaaattttt ataataatcc ttcttttgaa tttggaggat attgtcttcc caaagatact
      541 aaacaattaa aaaaagaatt tatagaaata aatgccccag tgatagaagc gattgatatt
      601 tcaaacacaa atagaaaaca gttcatagtt aaacaaatat tggaacgaaa gccaaagata
      661 gtaggaatat ataaattggg gatgaaatat aattcagata attacaaaga gtcagctatt
      721 ttaagtataa ttaatgaact attgattgtc ggtataaaaa ttttagtata tgaaccaaat
      781 ttaaacgtta gtatagataa cgtaattttt gaaaagaatt ttgaactatt tacaaaacaa
      841 agtgatctaa ttgttgcaaa tagatgggat aggggccttg aagcttataa agataaagtt
      901 tacacaaggg ggatctggat aagagattaa ctattgatat taaaaaggca ataatttata
      961 aaatttgtac aaaaaaatgt taaattttca cattttcata gtatactgtt taatagaaag
     1021 taggttcttt gtaaccgctc cattataaaa tcctttcact cttccggtat gctattagga
     1081 aaaacataat tggaggatat tttgagctca cacctcatcg ttccagtggc tgttccccaa
     1141 gctcgtgtac tgcccaaaaa agttagacat tctatttaga acgaggattg agttctgtat
     1201 tgtacaaggc tcggtccttt tagagtcagc ttaaggctgg ctttttcaat caccaaagtg
     1261 tcagaatgtt ttgacaaatg aacacaaata atgatataat atgcaaaagc taggaggtgg
     1321 taggatgatt gaaaaaatgg aactggggga attttacaag gaattacgtt tggcgagaaa
     1381 gctcaagcag tcagatgtgg cttgtgacgg gctaacagct tctcagttgt ccaagtttga
     1441 gctagggcag tccatgttgt ctgctgataa gctcatatta gccatccaag ggatcaatgt
     1501 gacctttgat gagtttgggc acaagctcaa caactatcaa gaatctccac atatgcagat
     1561 tggtagaaag gttgtggatc gctttgctca tcaagatata gctggcttag agcaactgtt
     1621 ggaggaagtc gagcaagaac agatggcaga gacttatcgt cgtttgaatg ctattgtaat
     1681 caaagatgcc atccactcac tagataaaag ctacccacta gcagaggagg atagcgagtt
     1741 tttgaccacc tatctctatg ctatcgagtc ttggacctgg tttgaactct atctcttttg
     1801 caatactctg cccttcttga gcaatcaaga tctgatcttt ttatcaacct ccttactcga
     1861 aaaatccaaa gaatttaaag agttagtaca caatcgattg tatatgaagc aaggactctt
     1921 aaatatctta tcagaactca tggagcgaaa acttttctta tacatcccaa tcttcgaagc
     1981 cgagttggag agcatgttgc gtccgtacga tgtttttgaa aaagtatcgt ggcaattttt
     2041 aaagaagatg agtgtctttc ttcaaacaaa gggaagcaat caaaaagaga ttgaacgctt
     2101 tacccaatct ctgcaggtat tagaaaatcc acaattaaca tccctttttg aattgcgttt
     2161 gcagcaatac aaaaagctta tcgattagtt agaaatggca aaataataat aatctcagca
     2221 acgatcgtca ataatgttaa aacttgtttc ttcatattac ttccctcctc attagtaata
     2281 actttattat accagggaaa taatcaaatc taccaaaatc gcaaatctga aatttctata
     2341 agaaaaaata tcaaatatgc gattttctaa aataagccaa tttccgtgtt atactgtcct
     2401 tgtaaaacat ttgaagcaaa tcctaggtcg cagaagtggt ttacaaatga agataattga
     2461 aggagtgtaa aagatgttaa atttacaatt tgcagaaaca atggaattga cagaagctga
     2521 gttggagaca ggttatggag gggaatttgg gaataatgct gttatcccag ccggtgcttg
     2581 gggaggttta ggaacgtctt ggtcaatcac taatttctgg aagaaatatt ttaaccatga
     2641 ttcttccact gttaatcgtc gccattattg atattcagag tccttgcctt tttcttgggt
     2701 agggactgtc tttatttcat agtataggag aaaatagaat gaaaaagata atctcacacc
     2761 gctactttat tatagttttt ctactagtta ttgctgacca gaagttcagt gttttagttt
     2821 tacgcagcga ccttgttgct gggctatctg actttgccta ttatctgtcg gatatgatgt
     2881 tgaattttct tgtggtttta tttgctctta ttgctatgat ttggtcgggg aaatggcaga
     2941 aaatcaatag tagaaagttt aaaggatcct atcttttcta ttccttccta gctctgcttg
     3001 cttttgttgc ttggaatttt gtcacctttt ttcttttccc acctactcga aatgaaattt
     3061 cttatcaaca tgctgctcct acttttacag gagctacggc atttttgatg tatttttttt
     3121 atcctgtgat tgcaggtccc atttttgaag acatgattta tcgtggatta gtgatgactg
     3181 ctctggaaaa aggaaagaaa tgggggctag atgtacttgg ttccgctgtt ttatttgggg
     3241 tctcgcacat tagtaatcac ggttgggtct tgacagactt tgtcttctat atgggtggag
     3301 gtcttatatt tgcagtctta tttagaatga caaagtcaat ttattggcct attggactgc
     3361 atatagtcta caatggcatt ggtcagcttt tgatgttact gtaattttgg ttgttagtag
     3421 tatcgcggtc tatccttggg gaaagattcc catttgaact taagaaggag ttgtgatgaa
     3481 agtatttctt caaaatagag attttaggca attaactatc aaccagtgga tttcaacgct
     3541 tggggatacg attttttatc tggcctttct gaattatgtg gcagatgcgt cttttgcacc
     3601 tttgacgatt ttactcatta cgatttcaga aacccttccc caaattttac aaatctttct
     3661 gggagttttg gcagattttc aacatcatcg tgtcttgaaa tacacagtca ttagttttgc
     3721 aaaatttttg ctttactcta tcgtttcttt atcactttca gggcagtctt tctccttgtt
     3781 gctagtagca tttatttgtc tgcttaacct cttgtctgac acattgagtt acttttcagg
     3841 agccatgctc actccgattt tcattagaat tattgggcaa gaccatctgg cagaagctat
     3901 tggctttaaa cagtcaactg ttagtttagt gaaaacaatc agtaatatcc taggaggagt
     3961 cttactaggc attctatcca tccagtttat ttccttactg aacgctctta cctttttaat
     4021 cgcattttta ggtatcctct tcataaaaac tgacctcttg aaagtagaaa aaacgattag
     4081 ctatcaagaa ggactctctg taaaatcctt ttgccagcat ttgctccaat catcaaaatt
     4141 gatatggaat atgaataagg tgctcttggt tttgtttatt atctctacta gtcaagcagt
     4201 gataaatgtt acggttccta tttccactct atttttaagg aaccagccct ttttgaattt
     4261 acaaacaggt caatctcttg ccttgctatc cacttttgaa ctgtcagccc tgattgtcgg
     4321 aagccttgta agtggctatc tgcaacatac catttccata aaaacagctc tctatgcctc
     4381 acttgtcatc cagttgcttc ttctagtagg atttgccaca gttcgttttg actggattct
     4441 catctttagt accttggatg cctttttcgc aggtgttctc tctcctagat tgcaagaact
     4501 cgtttttaaa caaatacctg aggagtcaat gggagcagtt caatcctcta tcggtgccat
     4561 tacggttgtt ttacctagct tatttacaat agctttggta accattgcta ctagctttgg
     4621 aactctggca gttagctttg ttttattgct atttcttcta gttgcctttg ttatgcttct
     4681 gaatattcgt gaaagtattt agtggagaat ttctataata tatgtttgag actggattgc
     4741 cagtcttttt atgatggtca aaatcaacag actttttctc ttttctgaat gtgttatagt
     4801 agactgaatc taaaatagta cgaaacaatt gctaaaacat ttatagaaat taattttact
     4861 ttcccaatcg atttgttctc atcttatttc aatctgctat ataatagtcc atgctgtggc
     4921 tggattcttt tcagcctact tgtactcttc gaaactctct tcaaaccacg tcagcttcac
     4981 tttgccgtag tacggttact gacttcgtca gttctatcca caacctcaaa acagtgtttt
     5041 gagctgactt cgtcagttct atccacaacc tcaaaacggt gttttgagca acctgcggct
     5101 agtttcctag tttgctcttt gattttcatt gagtattatt aagacaatat gaggagaaag
     5161 atgaaagaaa aaggatttta ggagggggcg caggcagcca tgccaacggc tcttggctat
     5221 gtcagtatcg gcctggcctg tggaattatc ggtgcgccct atgtgacact tgttgagatg
     5281 ggcttgatga gtctctttgt ttatgctggg agtgcccagt ttgccatgtt ggcactgatt
     5341 gtggttcaag ctcctgtggc agctattgct atgacggttt ttctaatcaa cttgcgtctc
     5401 tttttgttga gtttacacgc atcgacttat ttccgtcata ccagtctttg gtacaatatc
     5461 ggtatgtcta gtatcttgac agatgagacc tatggcgttt tgatgggtga attggcccat
     5521 acagacaagg taaatcctat gtggatgcac gggaacaatc tcaacagcta tgtggcttgg
     5581 tttgtgggga cagtagtcgg aacggctctg ggtggcctgc taccaaatcc agaaatcttt
     5641 ggcttggatt ttgccctggt tgggatgttt attggtattt ttgcttcgca atttcagatt
     5701 atgcaaagac ggattcctgt ccgcaatctg ctcattatcc tagcagttgt tgcggtgtcc
     5761 ttctttttgc tcttgacagt ggtgtctcag tcactagctg ttctgtttgc gacgctactt
     5821 ggttgtagca tgggggtggt tttagatggt cagtaagtat cttttattag cagttatttt
     5881 ctctggtctt gtgacctgga ttccccgtat gattcccttc atcttggtca agcataaggg
     5941 cctgcctgcg attgttgagc gttttttgaa gtttttgcct gtttccatta tctttgcctt
     6001 gattctttca agcgtagtga caggtaaggt tgggagcctt cctcaaatta aatggctaga
     6061 cttcttagcc gtctttccaa cagcttgggt agcctttcgc taccgcaatc tagtcggaac
     6121 agttctcttt ggagtggtct tgattgccat cttgcgtttg gtcttttaaa ttacccataa
     6181 aaaaacttat cacagagata gatatcatat aatggcgtaa atgctctttt tctgttaaga
     6241 ttataaggta ttctattttg gaggaaatga catgaaaaaa atcgttaaat actcatctct
     6301 tgcagccctt gctcttgttg ctgcaggtgt gcttgcggct tgctcaggtg gtgctaagaa
     6361 agaaggacaa gcagctagca agaaagaaat catcgttgca accaatggat caccaaagcc
     6421 atttatctat gaagaaaatg gcgaattgac tggttatgag attgaagtcg ttcgcgctat
     6481 ctttaaagat tctgacaaat ataatgtcaa gtttgaaaag acagaatggt caggtgtctt
     6541 tgctggtctt gatgctgacc gttacaatat ggctgtcaac aatcttagct acactaaaga
     6601 acgtgcggag aaatacctct atgccgcacc aattgcccaa aatcctaatg tccttgtagt
     6661 gaagaaagat gactctagta tcaagtctct cgatgatatc ggtggaaaat cgacggaagt
     6721 cgttcaagcc actacatcag ctaagcagtt agaagcatac aacgctgaac acacggacaa
     6781 cccaactatc cttaactata ctaaggcaga tttccaacaa atcatggtac gtttgagcga
     6841 tggacaattt gactacaaga tttttgataa aatcggtgtt gaaacagtga tcaagaacca
     6901 aggtttggac aacttgaaag ttatcgaact tccaagcgac caacaaccgt acgtttaccc
     6961 acttcttgct cagggtcaag atgagttgaa atcatttgta gacaaacgca ttaaagaact
     7021 ctacaaagac ggaacgcttg aaaaattgtc taaacaattc ttcggagaca cttatctacc
     7081 ggcagaagct gatattaaat aatttcttga aatcatccat tctgagtaag atgggtggtt
     7141 tctcagtttt tagggatata gttttaaact atatatctga ctatctaata acaatttgtg
     7201 aaatcaaaca aattgtgaga tactagtacg gtattatttt taaggagaaa gaatcatgaa
     7261 aatcaaaaaa tggcttggcc tagcagccct tgctacagtc gcaggtttgg ctcttgcagc
     7321 ttgcggaaac tcagaaaaga aagcagacaa tgcaacaact atcaaaatcg caactgttaa
     7381 ccgtagcggt tctgaagaaa aacgttggga caaaatccaa gaattggtta aaaaagacgg
     7441 aattaccttg gaatttacag agttcacaga ctactcacaa ccaaacaaag caactgctga
     7501 tggcgaagta gatttgaacg ctttccaaca ctataacttc ttgaacaact ggaacaaaga
     7561 aaacggaaaa gaccttgtag cgattgcaga tacttacatc tctccaatcc gcctttactc
     7621 aggtttgaat ggaagtgcca acaagtacac taaagtagaa gacatcccag caaacggaga
     7681 aatcgctgta ccgaatgacg ctacaaacga aagccgtgcg ctttacttgc ttcaatcagc
     7741 tggcttgatt aaattggatg tttctggaac tgctcttgca acagttgcca acatcaaaga
     7801 aaatccaaag aacttgaaaa tcactgaatt ggacgctagc caaacagctc gttcattgtc
     7861 atcagttgac gctgccgttg taaacaatac cttcgttaca gaagcaaaat tggactacaa
     7921 gaaagcactt ttcaaagaac aagctgatga aaactcaaaa caatggtaca acatcattgt
     7981 tgcgaaaaaa gattgggaaa catcacctaa ggctgatgct atcaagaaag taatcgcagc
     8041 ttaccacaca gatgacgtga aaaaagttat cgaagaatca tcagatggtt tggatcaacc
     8101 agtttggtaa taagaaacag ggaggtggga gagaaaattc cacctcttgc ttttgtatag
     8161 agtatagatt gtaaagaaga ctattcgttc atagaaaggt agagagaata tggtttttcc
     8221 tagcgaacaa gaacagattg aaaaatttga aaaggatcat gtagtccaac attattttga
     8281 ggttttgcgt accttgattt ctaagaaatc agtctttgcc cagcaggttg gactcaagga
     8341 agtcgcaaat tatctgggtg agattttcaa gcgtgttgga gctgaagtgg agattgatga
     8401 gagctataca gcgccctttg tcatggcaca tttcaagagt tcgcgtccag atgccaagac
     8461 cttgattttc tataaccact atgacactgt gccagcggat ggggatcagg tctggacaga
     8521 ggatcctttt acgctttcgg tccgcaatgg cttcatgtat gggcgtgggg ttgatgacga
     8581 caagggtcat atcacagctc gcttgagtgc tttgagaaaa tatatgcagc accatgatga
     8641 tttacctgtc aatatcagct ttatcatgga gggagcggag gaatcggctt caacagacct
     8701 agataagtat ttggaaaagc atgcagacaa actccgtggg gcggatttgt tggtctggga
     8761 acaagggacc aaaaatgcct tggaacagct ggaaatttct ggtggcaata aggggattgt
     8821 gacctttgat gccaaggtaa aaagcgctga tgtggatatc cactcgagtt atggtggtgt
     8881 tgtggaatca gctccttggt atctcctcca agccttacag tctcttcgtg ctgcggatgg
     8941 ccgtatcttg gttgaaggct tgtacgaaga agtacaagag cccaatgaac gagaaatggc
     9001 cttgctagaa acttatggtc aacgaaaccc agaggaagtt agtcggattt atggattgga
     9061 gttgcctctc ttacaggagg agcggatggc ctttctaaaa cgtttctttt tcgagccagc
     9121 gcttaatatc gaaggaatcc agtctggtta tcaaggtcag ggtgttaaga ctattttacc
     9181 tgcagaagcc agtgccaagc tagaggttcg tctggttccg ggcctagaac cgcatgatgt
     9241 tctggaaaaa attcggaaac agctagacaa aaatggcttt gataaggtag aattatacta
     9301 taccttggga gagatgagct atcgaagcga tatgagcgca ccagccattc tcaatgtgat
     9361 cgagttggcc aagaaattct atccacaggg cgtttcagtc ttgccgacga cagcggggac
     9421 aggacctatg catacggtct ttgatgccct agaggtacca atggttgcat tcggtctagg
     9481 aaatgccaat agccgagacc acggtggaga tgaaaatgtg cgaatcgctg attattacac
     9541 ccatatcgaa ttagtagagg agctgattag aagctatgag tagagatatt atcaagttag
     9601 atcagatcga tgtgactttt caccaaaaga agagaaccat tacagcggtt aaggatgtga
     9661 ccattcacat ccaagaaggg gatatctacg gaatcgttgg atattctgga gcaggaaaat
     9721 caacccttgt acgggtgatt aatctcttgc aaaaaccatc tgcagggaaa attaccattg
     9781 acgacgatgt gatttttgac ggcaaggtga ccttgacggc agagcagttg cgtcgtaaac
     9841 gtcaagatat cggaatgatt ttccagcatt ttaacctgat gagccaaaag acagcagagg
     9901 agaatgtagc ctttgccctt aaacactctg aactcagcaa ggaagaaaag aaggctaaag
     9961 tagctaagtt gttggacttg gttggtttgg cagatcgtgc tgaaaactac ccttcacaac
    10021 tatctggagg gcaaaaacag cgtgtggcaa ttgcgcgtgc cttggccaat gatccaaaaa
    10081 tcttgatttc agacgagtca acttctgccc ttgatccgaa gacaaccaag cagattttgg
    10141 ccttgttgca agatttgaac caaaaattag gcttgactgt tgtcttgatt acgcatgaaa
    10201 tgcagattgt caaagacatt gccaaccgtg tggcggttat gcaggatggg catttgattg
    10261 aagagggtag tgtgcttgaa atcttctcag accctaaaca acctttgact caagacttta
    10321 tctcaacagc cacaggtatt gacgaagcca tggtcaaaat cgagaagcaa gaaatcgtgg
    10381 aacacttgtc tgaaaacagt ctcttggtgc aactcaagta cgctggatct tcaacagacg
    10441 agccactttt gaatgaattg tacaagcatt atcaagtaat ggctaatatt ctctatggga
    10501 atatcgaaat cctcgatggt actcctgttg gagaattggt ggtggtcttg tcaggtgaaa
    10561 aagcagcgtt ggcaggtgcc caagaagcca ttcgtcaagc aggcgtacag ttaaaagtat
    10621 tgaagggagg acagtaagat ggaatcattg attcaaacct atttgccaaa tgtctataag
    10681 atgggttggt ctggtcaggc aggctgggga acagctatct acctaaccct ctatatgaca
    10741 gttctttcct tcattatcgg aggcttcttg gggctagtgg caggtctctt tctcgtcttg
    10801 acagcgccag gtggtgtctt ggagaataaa gtcgtattct ggattttaga caaaattacc
    10861 tcaatttttc gtgcgattcc ctttatcatc ctcttggcaa tcttgtcacc actttctcac
    10921 ttgattgtta aaacaagtat cgggccaaat gcagcccttg tcccactttc ttttgcagtc
    10981 tttgccttct ttgcccgtca ggtgcaggtt gtcttggctg aattggatgg cggtgtcatt
    11041 gaggcggctc aagcgagcgg agcgactttc tgggacatcg tgggtgttta cctatcagaa
    11101 ggtcttccag atttgatccg tgtgacgact gtgaccttga tttcccttgt tggggaaaca
    11161 gctatggccg gtgcggttgg agctggtggt atcggtaacg tagccatcgc ttatggattt
    11221 aaccgctaca atcacgatgt gaccatcttg gcaaccatta ttatcatttt gattatcttt
    11281 gcaatccaat tcttgggaga tttcttgact aagaaattga gtcataaata aaaagagccg
    11341 cgtggctctt tttaactgat cagattttct gtgcaaattt tttactcaag gcttgtccaa
    11401 tcaaggcacc cactagggct ccgatgacaa tacttgcgat aaatagaagg acagttccag
    11461 ggtttggagc gaccatgata cggtcgatat attcttggga ttttcctctt gccagaagag
    11521 tagccatata ggctttgggc gcaatccaca taagcaagat tggtcctgtt gtactaaagg
    11581 cgaaaataat gaaagaaagg aagttctttg ttttgtcctt gtattttcct aaatgagcta
    11641 cttcatctgc taggaggcca cagataattc caggaaggaa ggaaccagca ccgtgtttag
    11701 ttcccaagaa aaagagggca atgacaaggc cgatagtggt aatggctcca aagcgcggaa
    11761 cttttgcgac taggatcata tagacgctac cgccgacaag ggcagtaaag gcaggcgcat
    11821 aaaacatgtt tccagtttgg tcaaagagat tgcccaaaag gacaccaatc cccatgcaga
    11881 ggaagtaaag aactgcaaaa agcagtgtag ttaagataga tttcttcatg aattgtctcc
    11941 tgataatttt ttcacaattc tcattgtacc acggtttggt gggattgtaa atggatacta
    12001 gaagtttttg aaaacgcttg aaatatgata taatggtttc atagttattt ttaggggaat
    12061 atcatgggta gatttttaga ctttgtcttt aatcgtttct ttttagggat gattgcgaca
    12121 gccttctttt ggctattaac tttagcagga gggattatcc ttggtctagc gccggctagt
    12181 gccaccttga tgagcttata tgcagaacat ggttatagct ttcgggaata cagtttgaag
    12241 gaggcttggt ctctttacaa gcaaaatttt gtctcaagca acctgatttt ctatagcttt
    12301 ttaggtgtgg gtctagtttt gacctatggt ttgtatctct tggtgcaatt gcctcatcag
    12361 accattgttc atttgattgc gacccttttg aatgtcctag tagttgccct gatctttttg
    12421 gcttatacag tatctttaaa attacaagtt tattttgcct tgtcctatcg aaatagtctc
    12481 aaattatcct tgattggcat ctttatgagt ctagcagctg tggccaagat tctccttggg
    12541 actgtgctac ttgtagcaat tggttattat atgcctgccc tgctattttt tgtaggaatt
    12601 gggatgtggc atttctttat cagtgatatg ttggaacctg tctatgaaat catccatgaa
    12661 aaattggcga caaaatagaa tgaagcagtt ttggctacat acgcttctaa gaacctatag
    12721 ttcagtgatg atcattatca ttgcgagttt tgcaatctta ctctcttacg ctgactggga
    12781 ttcacgtgaa aaggaagccc agagagtagc ccagcgtgta actgctagaa cagtgagtga
    12841 aattgaatat taccatagag agtcaaccca gatagctcag gctttagttg aaaaccaagc
    12901 tcgtattgag ggaatctata aatactttag tcttagcatg ccagactatt tttactggca
    12961 attagagcgg aaagcttcgc cttatatatc agtctctctg tatgaaaatg ttgatgacct
    13021 ctatgttcga aatgattttg taactggagt ggccattgct tttcaagatt acaaggaagt
    13081 ctatgtttct actaaagaca aacgtagtgg agaaaaaatc agggctgagg atttcaaacc
    13141 agcaggaaat agttttgcca ttccggtgtc agatccagtg tcagatcaag atttaggagt
    13201 gatttacatc tccttggatc ctgctgtttt ataccatgcc attgataata ctagaggtca
    13261 tactccgatg gcagtaacag tgacctcacc ttttgatacg gagatttttc atattggtga
    13321 gacagttgat aaggagagtg aaaattggct agttggctta acttctcatg gatatcaggt
    13381 tcaggtggca gttcctaaaa actttgtttt acaaggaaca gtgactagct ctgctttgat
    13441 tgtgggtttg agccttctct ttattgtcat tctttatctg actttgaggc agacttttgc
    13501 taattaccaa aagcaggtag tggatttagt agaatccatt caagtcattg ctcaaggcga
    13561 agaggggcgt cggattgaca tttccgagaa agatcaggaa ttactcctaa tcgcggagac
    13621 gaccaatgat atgttggatc gattggaaaa gaatatccat gatatttacc agttagagct
    13681 tagtcaaaaa gatgccaata tgcgagcctt gcaggcgcaa atcaatcctc attttatgta
    13741 taatacgctg gagttcttgc gcatgtatgc agttatgcag agtcaagatg agttggcaga
    13801 tatcatttat gaattcagta gtctcttgcg taacaatatt tccgacgaaa gagagaccct
    13861 cctcaaacag gaattagaat tttgccgtaa atacagctat ctctgcatgg ttcgctatcc
    13921 caagtccatt gcctatggtt tcaagataga tccagagtta gagaatatga agattcccaa
    13981 gtttaccttg caaccgctgg tagaaaacta tttcgcgcat ggtgttgacc acaggcggac
    14041 agataatgtg attagcatca aggctcttaa acaggatggt tttgtggaaa ttttggtggt
    14101 cgataatggt agaggaatgt cggctgaaaa gttggcaaat atccgagaaa aattaagtca
    14161 gagatatttt gaacaccaag ccagctacag tgatcaaagg cagtctatcg ggattgtcaa
    14221 tgtacacgag cgttttgtgc tctattttgg agaccgctat gccattacta tagagtctgc
    14281 agagcaagcc ggtgttcagt atcgtattac aattcaagat gagtagaaag ggagaaaatg
    14341 tataaagtat tattagtaga tgatgagtac atggtgacag aaggtctgaa gcgtttgatt
    14401 ccctttgata agtgggatat ggaggttgtc gcaacagcca gtcatgccga tgaagctcta
    14461 gaatatgttc aggaaaatcc tgtcgatgtc atcatttccg atgtcaatat gccagacaaa
    14521 acagggcttg atatgattcg ggagatgaaa gagatcttac cagatgctgc ctatatcctg
    14581 ctctcaggtt atcaggagtt tgattatgta aaaagagcaa tgaaccttag tgtggtggac
    14641 tatttggtca agcctgttga taaggtagag ctgggaaatc tgctggagaa gattgcaggt
    14701 cagctcggcg agagagggaa gaaaagtcag actcttagtc aagaattaga cgaggctgga
    14761 tttgttagtt atttagggga taaggagaat tggtggatag gtctatccaa ggaaaaacaa
    14821 ggttccttca ccattcccta ctatgtcttg ggtcaagact ggcagatttt catttctggc
    14881 caacccttag atggcttagt cgttactccc tttgaagctc cctatcaaga acactttgaa
    14941 cgctggaagc tgaatgctga gaaaaccctc ttttacggtt ctgtaaatct gcagcagtct
    15001 gagagtctct ttgcctatta cgaaccgatt tatagggtta tcattcaggg aaatctcaat
    15061 caaatcgtag aagagttaaa tctcttggag aaggtagttc ttgaaaatac gccgcgtgtt
    15121 ccgattacta aacagctttt tattcagttt gtcatggatg ttttccattt atttgaacat
    15181 ctcaaagctg atgacctgac agacatcgtt aaaaccattc atgctattca atccttcgat
    15241 gaattggttc cttatatcaa ggaaactctg accagctttt tcggtcaata ccgtatgaat
    15301 gaaaatgtgg tcagtgtgct ggaagtcatt ggtcgtgatt accaaaaaga gctttccctc
    15361 aaggatatca gtaaggctct ctttatcaat cctgtctatc tagggcagtt gattaagcgt
    15421 gaaaccgatt cgacttttgc agagttacta aacaaacaac gtattaaggc tgcccagcag
    15481 ctcttgcttt caactagtga cagcatcgaa gatatttgtt atgctgttgg ttacagtaac
    15541 cttggatatt tctataaagt tttccgaaaa ttgtgcggaa aatcgccaaa agcctaccga
    15601 aaacaggtag aaactatact ataagatttg tattccttta caaaatgtgc tataatatta
    15661 tcaggaggtt ctatcatgaa aaagaaacca atttatctat gggtcttgct aatcttgtct
    15721 gcccttattt cagttccgtc tctgtttgga attgtgagtc ccttgcctag taaagaagcc
    15781 cttcgtgctg ctcagaaaca agttgcaggg gtcaatgctc agcaattaga agaccagctt
    15841 aattatacct atagagtagc agaagcatct cattctattt ttaatgttgc tttgattgtg
    15901 ctatctacta ttttagtggt cgtagcgatt gtcttccttg ttcgtaaaaa tttgcaatat
    15961 gcaaactata cttacgttgg ctatgttttg ttagctatta ttggttcgat ttatggctat
    16021 gttggtttac aagatgctgt gcagttggtt caagatgaga gcatgcgttt gacagtgagt
    16081 attggttcaa aagctgttag cattttctat atcgtcatca atgttctctt cctagccctt
    16141 gtcttctata agatgtggcg acaacagaaa gccttggcag aagaagagga aacagaagag
    16201 cttgcctaag tattgacaaa aaagaactat caagatacaa tcttggtagt tgttttttga
    16261 ttatagataa aatcatggag gtacatatga agacaatttc tttagtttat attagtttga
    16321 gtggcaacac tgaaagtttt gtgacgcgct tgaaagacta tctcttgtcc cagtacaaag
    16381 ggattgaggt tcaaaagatt catatcaagg atttggtcaa ggaaggcaaa aatttctacg
    16441 aaatggacca tccctatgtt gcttttttgc cgacctatct cgaaggtggg aatggcgtgg
    16501 ataacggaga tgttgagatt ttgacgacac cggtgggaga ttttattgcc tatggtaaca
    16561 atgctagtaa gtgttttggt gtggttgggt caggaaatcg taactttaat aaccaatact
    16621 gcctaacagc caagcaatac agtcaacgct ttggctttcc tgtattggct gactttgaaa
    16681 tgcgaggcat gctggaagat atcaaacatg tcgcagctat tatcgcagat ttgtatgagt
    16741 tggaaaagga gaattaatac tcaatgaaaa tcaaagagca aactaggaag ctagtcgcag
    16801 gttgctcaaa gcacagcttt gaggttgcag atagagctga cgtggtttga agagattctc
    16861 gaatagtata aagttcttat aagaggtggt tgaagttgtt caactgtttt ttgagtataa
    16921 acagtcttta agaacgtaaa aaagcataag ttcaggtctt caaaaccttg ttcttatgct
    16981 ttttatcata gaaatatttg ctgaattttc tcttgaaatg aaaccttatc ttcatctcgc
    17041 gcttgtccct taccctgctt ttccgattgc gagtgcgtag ataaagaaga tggcgaagcc
    17101 aaagaggaaa atcaatcccg caatggcaag gtgtttgagc caagaaggaa gatttttgtt
    17161 ttcacgtgtt accaaggcgt aattcaaaag agcaaagaaa ggtgttgtca ggaaagagcc
    17221 aatcatggca aagcggagca tggttgaaac ctgaccagcg aagaacttga taatgatgat
    17281 accgatgata gcagtgatgg tcatccagat gttcaaagat ttacgattat cctctttttg
    17341 actgattagc agtcggagag attcctgatt aacgcgagaa tagccatcga taacagttat
    17401 aactgttcca aagatacaga ggaaggcaat aaaggtaatc aagtaacggg accattcgcc
    17461 aagaacagag gcatacatgc ccacgaattg agagatgtat ttggctgaag cagcttcaac
    17521 cgcctgccct gtaggatact gaatcagtgc tcccagtgcc acaaagaaga cggctaggat
    17581 agctgttcca atataaccag tgttaaagtc aaacagagcg tcttctgtgt taaagttgac
    17641 ggtctttctc ttttcagctg accaaagtga attgatggct gaaatttcaa taggagccgg
    17701 catccatcct aggagggaga cgatgaaggg cagagctgcc atttgccaag gtgtcttctc
    17761 gacaaaatca gaactgtatt ctggatgctt gaccgccgca atgataactg caagaacagt
    17821 cgcaatggtt aaggcagaca taatccattt gaccatgccg tctaaaagtt tgtagcctcc
    17881 aaagagtagc atagcccaaa tgattgcaac gagaatgagg gaccactgag taatgctaag
    17941 tccaatcatt gggaaggcac tggcgatgat agctgagcac agaatggcaa caccagccgt
    18001 gttgaccata gccgaaaaga cattgaggat aaagaaaatc cagagataga gttttccttt
    18061 ttcggcataa ccttcaacca aagtctttcc agtatcagct gtgtattcag caccaaaacg
    18121 gaaaaatgga tatttaaaga cattggctaa gatgaccaag agaagtagag accaaccgta
    18181 agaaccgcca gcttgagttg aggatacaat gtgggaacct ccaacagcgg cagttgccat
    18241 taggattcca ggtcccattg cttttagctt gcttgcccag gttgattgat ttaaggaaat
    18301 agcttgcgac atgataaata ctcctttttg aattttcaga ataatctgta tatgtaatct
    18361 atattctaca aaaaattttg gaaaaatgca agagtatttt gaaaaaagat agaaaattta
    18421 caaagaaaat ctgaaataac tgttatgata tagaggattg aaactagaat agtacacctc
    18481 tgcttctaaa acattgttag aaatcgattt gactgtcctg atcgatttat cctgttctta
    18541 tttcatttca ctatagtaag gtgcagcagg ataaagaaaa accgagaagt ttctttctcg
    18601 gatttgctta ttatgaggtt cctattttct atgctccaag agttgattga tgtgatctac
    18661 ttttttagcc tctcttttat agagaatagc ccagatgatg agatagacaa tcgcaaactc
    18721 gataatgagc tggaggtaga aaatccagtg gaaagggaac caaccagcaa gagttgctag
    18781 tgggacaaag ccagccagca taaggaagaa atgagtcaga gtggcacgaa gcatgctcca
    18841 gtcacggcta aataagcggt tgccaaagtt gaagagcata ccgatggttg cccagataag
    18901 tgtgcagtag agcaagacca gggcaccgtg aacctgatgc tgatccatca cttggccgat
    18961 gagagagtag ggatttagtg gtgcgtaggt atttggtgca taaatgagtg aaaagaggat
    19021 agagaggatg aggccgataa gaacaccggt agctgcatcg tgaaatactt gttttttcat
    19081 agttctaatt tctccttgat ggtttttaga taacggcgtg aagagtaggt gaagctttcg
    19141 tttttcaaga aaatttctac cagaccgttt ggcgtgagct tgaggtgaga gatggaatcg
    19201 atattgatga tttctgattg ggaaatttgg ataaaattgg ttggcaagag tttaagaacc
    19261 tgatagagtc gcaaatcaat gctgtaggtc tgactcgcgg tttctgctag aaccttccga
    19321 ttctcgatat agaagcgctg aatcttgcca atctcaacta gatagacctg atcatcgatt
    19381 tttcctttga ttttttctct ttggtccaga ttttctgcga actcgatgac tttctggact
    19441 ttttcggttt cttgaggtgc ttggacaatc agcttttcct cctcgtaagt ctcactaatc
    19501 tgtagttcta ctttcatagt tttctctcct tttcagttat acaaggttgt gatcacttcc
    19561 tgtatatcct tattaaacac tattttatag cccatggcaa ggaaccttgt ctatgtggag
    19621 ggattgggct cctatgtggt ggagcttttc tgttctttct gaaatatggt ataatagcac
    19681 taatcaattt ctaggaaaat agatacggaa aggggctgaa agaactaggg gaacagttgg
    19741 aattagaaag tgataggtat gaaaagtggc gttgaagctg aataagttta taaagcgttt
    19801 gaatgaagaa atggaaagta aataagttga cgatgaatta caaaatagat tttatctaca
    19861 atttgtatct ttcaaatcat tgatttgaat tttagattta atcatgttac aattaaaact
    19921 gaaatcaaat agaagaggaa aatgcaaacg tgggcacact attagcaaca agattaaaaa
    19981 atagacggaa agaattaaaa atgtctcagc gagaactagc tgaaggtatc tgtaaacagg
    20041 ggcaaatcag tcgattagag agcggagagt ttacccctgg agcggatttc ctgaatgccc
    20101 ttgctaagaa gttaaaagtc agtatggatt atttttttga tgagcaggtt gtagagaaag
    20161 ttgaggagtt atcagaattt aagaaattag cacagacatt tattacaaat cgcaattatg
    20221 agtctttgaa atatatatat gaattggaga atgtaaaagc tcaccgccta tctttagcag
    20281 ataaaatcta catggaatgg ataaaatctc tgatagattt ttatttttac ggacagaaag
    20341 aagatgcgat tgtacgatta gagggggtat tatcacagtt aagtgtatct gatttgaact
    20401 atctccaagt ttcaaacacc ctattcaatt tttattatga tattggagat ttaggtcgtt
    20461 tcaataaaat tcgtgaaaaa ctagagtatc aagtaaatca cctcaatttg agtacaatcg
    20521 aagagttaga actatctatt aagtttaact acaacgtttg tcgttatcta tggttacaga
    20581 aaaatattga agaagctatt actaaaatta cagctactat aaaacaatgt aaggcttaca
    20641 gaacgaatta tcttttggct gatttgtact tattgatggg aaatgtaagt gaaaattttt
    20701 cttctaaaag ttcggtgaaa gaatattttg agacggcata ttttctatac aaacttgagg
    20761 agaacatgtc aatgactctg aaagttgagc attacattgc agatataaca gaataatgaa
    20821 tacggtgttc aagtgagcat cgtttttcaa acctagttca gatacaaaaa agtatcaaac
    20881 aaaaaaaaat aaaaaaacat tataaaaaca attgacaagc taattttgaa gtgataaaat
    20941 aaatgcaaga aaacaaaagg agaggtatat gagaagtttg tttaagaaaa tagttgctgt
    21001 attagttatt ggcttaattc tgttaggaat tgcgagaaca cctcaagttc ataagatggc
    21061 tagagggatt gatcctggtc ctgcgaattt tatttagaaa tcaatttcta ctaattgagt
    21121 actaattgag tattaatggg taaaaagatt gtatggataa gaaaaattta cttgtattgg
    21181 ttactggttc ggtaggtgcc agtaatgt
//
LOCUS       DAVKQB010000040        20530 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKQB010000040 DAVKQB010000000
VERSION     DAVKQB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.040"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            21..1796
                     /locus_tag="VW528_001511"
     CDS             21..1796
                     /locus_tag="VW528_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEU9912319.1"
                     /translation="MANNRKTETLGVSYLSTFIDKHELLQSYFESNDKTPVWDGEIHV
                     LKSPSEKKDEILGKVPVQIKTTRQKKDVLKSFSLDTRDLELYKPNGGVVLFVVWLNED
                     NGLRDIYYKSLPPLSIKNLLKKSKLKNKSTNRKKLSIEIFKLDEKKMYPMLVDFINNS
                     QKQYSFINVEGISVEDIPDDKTLKFYFYGQEKEEIFNYQEEHDLFIYYLDPITGIEIP
                     LENTIKIVETEEETDLIIKIGDYVFQDVKRHRFPDGSVQLHFGESFTMSFDIKKKQFK
                     FNYTRPDLLSKAIKCTQVFQELGKIGYFTLNGNKIELDERSIKDISSLDLEADIKGLL
                     KISNFMKKMGIQKDVDLSCFDKQSQRNLNILYSGLVLKKKVALNYNESKLLHLNIANI
                     HIITLYSFLSDKNGTMIDIFTETPWCREGETEDEDYLDISIFEVFEPNDWLKIDNCKI
                     DSVIASYQRLVDNKLKYEGADRTILKIVIAADMAEDKTKRELLLNWAQCLSDWNLKYS
                     KNCEMAIINDLQIKSRVRKLNSKETETLTNILVNSNDNYELCFGSSVLLKSKPQADLF
                     WNKLDNETKERYKDFPIYTLYMKLS"
     gene            complement(2007..2813)
                     /gene="yidA"
                     /locus_tag="VW528_001512"
     CDS             complement(2007..2813)
                     /gene="yidA"
                     /locus_tag="VW528_001512"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9912320.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMADKEIVKCMFIDEPE
                     ILDATIEKIPAEFYERYSINKSAPFYLELLTKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(2826..4130)
                     /locus_tag="VW528_001513"
     CDS             complement(2826..4130)
                     /locus_tag="VW528_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9912321.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHAYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            4202..4579
                     /locus_tag="VW528_001514"
     CDS             4202..4579
                     /locus_tag="VW528_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9912322.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSRYQVDGQKIE
                     LAYQLQNQEGHPFASYQLEITWG"
     gene            4669..5001
                     /locus_tag="VW528_001515"
     CDS             4669..5001
                     /locus_tag="VW528_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9912323.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            5013..6584
                     /gene="ffh"
                     /locus_tag="VW528_001516"
     CDS             5013..6584
                     /gene="ffh"
                     /locus_tag="VW528_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9912324.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(6779..8062)
                     /locus_tag="VW528_001517"
     CDS             complement(6779..8062)
                     /locus_tag="VW528_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9912325.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMLATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTSGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(8316..9029)
                     /gene="rsmG"
                     /locus_tag="VW528_001518"
     CDS             complement(8316..9029)
                     /gene="rsmG"
                     /locus_tag="VW528_001518"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9912326.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            9123..9683
                     /locus_tag="VW528_001519"
     CDS             9123..9683
                     /locus_tag="VW528_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9912327.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            9685..10584
                     /gene="htpX"
                     /locus_tag="VW528_001520"
     CDS             9685..10584
                     /gene="htpX"
                     /locus_tag="VW528_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9912328.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(10733..12292)
                     /locus_tag="VW528_001521"
     CDS             complement(10733..12292)
                     /locus_tag="VW528_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9912329.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEICSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(12531..12848)
                     /locus_tag="VW528_001522"
     CDS             complement(12531..12848)
                     /locus_tag="VW528_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9912330.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FGF"
     gene            13018..13560
                     /locus_tag="VW528_001523"
     CDS             13018..13560
                     /locus_tag="VW528_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9912331.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            13560..14189
                     /gene="nth"
                     /locus_tag="VW528_001524"
     CDS             13560..14189
                     /gene="nth"
                     /locus_tag="VW528_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9912332.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            14400..14921
                     /gene="pyrR"
                     /locus_tag="VW528_001525"
     CDS             14400..14921
                     /gene="pyrR"
                     /locus_tag="VW528_001525"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9912333.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            14940..15863
                     /locus_tag="VW528_001526"
     CDS             14940..15863
                     /locus_tag="VW528_001526"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9912334.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            15913..16992
                     /gene="carA"
                     /locus_tag="VW528_001527"
     CDS             15913..16992
                     /gene="carA"
                     /locus_tag="VW528_001527"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9912335.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            17305..20481
                     /gene="carB"
                     /locus_tag="VW528_001528"
     CDS             17305..20481
                     /gene="carB"
                     /locus_tag="VW528_001528"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9912336.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDTLIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIKSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         6144 a         3840 c         4510 g         6036 t
ORIGIN      
        1 gttattttag aggtgcagat ttggccaata ataggaaaac tgagacatta ggtgtttcat
       61 atctttcaac atttatagat aagcatgagt tacttcaatc ttattttgaa agtaatgata
      121 aaactccagt atgggatgga gaaattcatg ttcttaaatc accgagtgag aagaaagacg
      181 aaatattagg taaagtacct gttcagatta aaactactag acagaaaaaa gatgtgttaa
      241 aatccttctc attagatact agagatttag aactatataa accaaatggt ggagtagttt
      301 tatttgtagt ttggttgaat gaggataatg gtttaagaga tatttactat aagtcattac
      361 ctcctctttc tataaagaat ttgctcaaaa agagtaaact aaaaaataaa tctacaaata
      421 ggaagaaact ttccatagag atttttaaac tggatgagaa aaaaatgtat ccaatgttag
      481 tggatttcat aaacaatagt cagaagcagt atagtttcat aaatgtcgaa ggtatttcag
      541 ttgaggacat tcctgatgat aaaactctaa agttctactt ctatggtcag gaaaaagaag
      601 aaatttttaa ctatcaggaa gaacatgatc tctttattta ttatttagat cctataactg
      661 gcatagaaat acctcttgaa aacacaataa agattgtaga aactgaagaa gaaacggatt
      721 taattataaa aataggcgat tatgtttttc aagacgtaaa aagacatcgt tttcctgatg
      781 ggagtgtaca gcttcatttt ggtgaaagct ttacaatgtc ttttgacata aagaagaaac
      841 aattcaaatt taactatact aggccggatt tgctgtcaaa agctataaag tgtactcagg
      901 tatttcaaga gctaggaaaa atcggatatt ttacccttaa tggtaataaa atagagttag
      961 atgaacggtc aataaaggat atttcttcac ttgatttaga ggctgatata aagggattgt
     1021 taaaaatttc taactttatg aaaaaaatgg gtatacaaaa agatgttgat ttatcttgct
     1081 ttgataaaca atctcaaaga aatctaaata ttctttattc aggtcttgtc ttaaaaaaga
     1141 aagttgcttt aaattataat gaatcgaaat tacttcacct aaatattgct aacatccaca
     1201 ttataacttt atatagtttt ttgtcagata aaaatggaac tatgatagac atttttactg
     1261 aaactccttg gtgccgggaa ggagaaactg aagatgaaga ttatttagac atttcaattt
     1321 ttgaagtatt tgaaccaaac gattggctaa aaattgataa ttgtaaaatt gattcagtta
     1381 ttgcctcgta tcaaagattg gtagataata agctgaaata tgaaggcgct gaccgtacta
     1441 ttttaaaaat agttatcgct gcagacatgg cagaagataa gactaaaaga gagttgttac
     1501 ttaattgggc gcaatgtctt tctgactgga acttaaaata ctcaaaaaat tgtgagatgg
     1561 ctataataaa tgaccttcaa ataaaatcta gagttcgtaa attaaacagc aaagaaacag
     1621 agacactaac taatatttta gtaaatagta atgataacta tgaactttgt ttcggttcat
     1681 cagtgctatt aaaatcgaaa ccacaagctg atttgttttg gaataaactg gataatgaga
     1741 caaaagaaag atataaggac tttcctattt atactttgta tatgaaatta tcgtaaaatt
     1801 aaaagagatt ataaagagat aacatataga aatagagagt gatttattac aattcagtac
     1861 cacaaattaa gaaagcaatg cctatttttt gtactctcaa gatttaagta actgtacaac
     1921 tgcaattaaa tttattaatt tactctcatt gtgatatatt catacaaaaa ccgctcagct
     1981 gagcggtttt agtctattct ttccttttac agtacccatg ttcggatggc gtgggcaacg
     2041 ccggattcgt catttgtttt agtgatgtat ttggcgattt ttttgatttc tggatttcca
     2101 ttttccatga caacggggtt tccaacgact tccagcatgg cacggtcatt ttcttcgtca
     2161 ccgattgcca tggtttcatc tttggtcaat ccgagttttt cagccaagtg agtaatggct
     2221 gaacccttgt ctacattctt tgtaaggagt tcgaggtaga aaggagcaga tttgttgatg
     2281 gagtagcgct cgtaaaattc tgctggaatt ttttcaatcg tagcatcgag gatttctggt
     2341 tcatcgatga acatacattt gacaatttcc ttgtcagcca tttcttcagg tgtacggtag
     2401 aagataggca tgctgacgag ggttgattcg tgcacagtgt attttccgat attgcgattg
     2461 gcagtataga taccgtcctt ggtaatggcg tgcatgtgga caccgagctt gcggctgaga
     2521 aattccatat ctagataatc ctcataagtc aaggattcgc tgataatctc atgtcctgta
     2581 gcagtttctt ggacaagggc accgttgaag gttaccacat agtccccctc gtctctcaac
     2641 tgcaagtcgt ctagaagttt ggcaacgcct gcgatagggc ggccagttgc aatcacgact
     2701 ttgacaccag cttctttggc atcttggatg gcagaaaaga cttcaggagt gatttccttt
     2761 tggctgttga ctagggttcc gtcaatatcg acggcgatta gtttaatact cataaatttc
     2821 ctcttttagt ttttatttgg ggtaaaatga tcgttctcaa tcaagtgtaa aaattgctgg
     2881 gtgatgcttg cgaagatgct gttttggtct aacatttctt tcgggaaata aaagcgatta
     2941 tctccgtggc gacttccagc aagggattgg acgatagggg acaggctaga gagttctgcc
     3001 agttctccat ttttttgtaa aatctcaatc tgtgtccttg gattttcaga ttcgggacgg
     3061 taaatatcat aagggaggtc aaagttctta tgaatggcag tatagtagtc gggatcaaag
     3121 ccgatgtcct caaccaattt tctcatgcta gcgagttggt cttggtcttc ttgtgaaaag
     3181 gtaatggatt taaagacctt gcggttgaca aagcgatgcg ataaatctgc aagaatcttg
     3241 tcaggactgg tcatccagag ttggaagtag gtattcatca caccatcatc cagagccaga
     3301 tagtcagtca aggtcacatt tttttcgaag aaaggcagga ggtgtggaga agttcgggca
     3361 aagaaatcct tgtcctcagg atagagttcc ttggcgcgtt tgagaagatt ctgtaggaga
     3421 acttccatgg cgcgtgttgc ggggtggaaa taaacctgca tgtacatctg gtagcgactg
     3481 aggacgtagt cttcgatggc gtgcatgcca ttgcgctgaa aggcgatacc attttcgata
     3541 ggacgaatga ctcggaggat tcgagtcagg tcaaattccc cataggatgc tcctgtaaaa
     3601 taggagtcgc gcaagagata gtccatgcga tctgcgtcaa tctgactaga aatgagctgc
     3661 acgacctgct tgttaggata ggcatggtct atgacactgg caactttctc tggaaaatcg
     3721 ggcgctactt gtagcaggac ttggtgaatc tctgtctcag gattttgaat aatctcctga
     3781 gtaatggctt catggtctgt atcaaagaga ttttcaaaag tatgggagta ggcaccatgc
     3841 ccaaggtcgt gtaggagagc agcggtcatg gtcaagagag actcggcagg attccattcc
     3901 tcaggatatt tttcttcgaa aatctctgtg atgcgtcgtg caatttcata gactcctaga
     3961 cagtgagaga agcgactgtg ttctccaccg tggaaggtat aactggaagt tcccagttgt
     4021 ttgatccggc gcaaacgctg aaattctttt gtattaatca agtcatagat gatttgatta
     4081 ttgacatgga tgtagttgtg aacagggtca cggaatactt tttcgttcat atgaccatta
     4141 tagcaaaatc ctgctctttt tggtaaaata gtaggacaag agacaagaaa gagggcctga
     4201 tgtgaagatt cggatgcgaa atacaattca gtttgatgag cagttggaag tgattgacca
     4261 gctttatgat gtggaagtgc atgaaaaagg agattatagc tacctgcttt tctataatga
     4321 ggaaaaggaa aaagtagtga ttaaatttca tggtcaagaa cttatgatga gccgattttc
     4381 caatcccaag accattatgc gctttttaaa ggatagtgat agtttggcct atattcctac
     4441 ccctatgggc atgcaggaat ttatcattca aacgagccgt tatcaagtag atgggcaaaa
     4501 gattgagcta gcttatcaac tacaaaatca agaggggcat ccctttgcca gctatcaatt
     4561 ggaaattact tggggctagg atctcgtttt tctcaaatct actttctatc ttcaaggatt
     4621 ggctccctag tgtggtagat taggctattt tttggtataa taaaagttat ggaaatcgaa
     4681 aaaaccaatc gtatgaatgc gctctttgaa ttttatgcgg cgcttttgac agataagcaa
     4741 atgaattata tcgagcttta ctacgctgat gattacagtc ttgctgagat agctgaggag
     4801 tttggtgtta gtcgtcaggc tgtctatgac aatatcaagc gaacagaaaa gattctggaa
     4861 gattatgaga tgaaattgca catgtactcg gactacattg tccgtagtca gatttttgac
     4921 caaatcttgg agcgctatcc caaggatgat tttctgcagg agcagataga aattttaaca
     4981 agcattgata atagagaata agaggaagaa aaatggcatt tgaaagttta acagaacgtt
     5041 tgcagaacgt ctttaaaaat ctacgtaaaa aaggaaaaat ctctgaatct gatgtccaag
     5101 aggcaaccaa agaaattcgc ttggccttgc tcgaggccga cgttgccttg cctgttgtaa
     5161 aggactttat caagaaagtt cgtgagcgtg cagtcgggca tgaggtcatt gatacactta
     5221 atcctgcgca acagattatt aaaatcgttg atgaggaact gacagccgtt ttaggttctg
     5281 atacggcaga aattatcaag tcacctaaga ttccaaccat catcatgatg gttggtttac
     5341 aaggggctgg taaaacaacc tttgctggta aattggccaa caaactcaag aaagaagaaa
     5401 atgctcgtcc tttgatgatt gcggcggata tttatcgtcc agctgccatt gaccagctta
     5461 agaccttggg acaacagatt gatgtgcctg tctttgcact tggaacagaa gtaccagctg
     5521 ttgagattgt acgtcaaggt ttggagcaag cccaaactaa tcataacgac tatgtcttga
     5581 ttgatactgc gggtcgtttg cagattgatg agctcctcat gaatgagctt cgtgatgtga
     5641 aagcattggc tcaaccaaat gaaatcttgc ttgtcgttga tgctatgatt ggtcaggaag
     5701 cagccaatgt tgcgcgtgag tttaatgctc agttggaagt gactggggtc atccttacca
     5761 agattgatgg cgatactcgt ggtggtgctg ctctgtctgt tcgtcacatt actggaaaac
     5821 caatcaagtt cactggtaca ggtgaaaaga ttacggacat tgaaaccttc cacccagacc
     5881 gcatgtctag ccgtatcctt ggtatggggg atatgctcac tttgattgag aaagcttctc
     5941 aggaatacga tgaacaaaaa gcccttgaaa tggctgagaa gatgcgcgaa aacacctttg
     6001 attttaatga tttcatcgat caattagatc aggtgcaaaa tatggggccg atggaagact
     6061 tgctcaagat gattccaggt atggccaaca atccagccct tcaaaacatg aaggtggatg
     6121 aacgccagat tgctcgtaaa cgtgccattg tgtcttcgat gacacctgaa gagcgtgaaa
     6181 acccagattt gttaaatcca agccgtcgcc gtcgtattgc tgctggttct ggaaatacat
     6241 tcgtcgaagt caataaattc atcaaggact ttaaccaggc taaacagctc atgcagggtg
     6301 ttatgtctgg ggatatgaat aaaatgatga agcaaatggg gattaatcca aataaccttc
     6361 ctaaaaatat gccaaatatg ggaggaatgg atatgtctgc ccttgaagga atgatgggac
     6421 aaggcggtat gcctgactta tcagctctcg gaggagcagg aatgccagat atgagccaga
     6481 tgtttggtgg cggtttgaaa ggtaaaattg gtgaatttgc catgaaacag tccatgaaac
     6541 gtatggctaa caaaatgaag aaagcgaaga agaaacgcaa gtaagacaag agattgatgc
     6601 aaatctcatt tgatgagaaa ataaagtaaa aatttctatg ataaaacgta cagtatcaag
     6661 gttttagaat atctgatatt atacgttttt tgattttaat aattttgtta agacactttt
     6721 attatagtat acgaaaaatc cactcagctg tctgagtgga ttaggtgtat ttagactctt
     6781 agtctttatt ttcgtatggc aagatcaagt tcaagatgat tcctgtcatg gctgaaaggg
     6841 cagtacctga aagtgtaact ggaccaagtt taaggatagc tcctccaagt ccaagaacca
     6901 acatagcact tgcgatgatg aggtttcgca tttgagcgaa atcaacacgt tctttaatca
     6961 agactttcaa accattgctg gcgataaccc catagagaag gattgacata ccaccaagta
     7021 cagcgtttgg aatagttgaa atcaaggcag tgaatttacc aaggaagctg agggcaatcg
     7081 cgatgaaggc agcgttacgg ataactgaga cagaagcgat acgagtcata ccgataaccc
     7141 ctgtattttc tccgtaagtt gtattggctg gtccaccaag gaaggcagaa acagaagttg
     7201 cgataccgtc accaataaga gtacggtgaa gacctggttc ttttaagaat tgacggccac
     7261 agatttgacc caaaacagta tggtctccga tatgttcaga aattgttacg atagcgattg
     7321 gcaagatagc gatggcttct ggaccaaagt aaagattgta ctctttaaag gcaccacttg
     7381 tgctaaatgg caagtagaaa ccaggaattt caaaccagtt agcttctagg acaggcgtaa
     7441 agtcaaccaa accaagagtt agtgcgaaga ggtaaccacc gataatggca aagaggaatg
     7501 gaatgattcg taggaagcct tttccttttg tattgataaa ggcagcaatt aggaaagtaa
     7561 caacggctac cagagcattt ttccaatttc cgtctgctac aagacctgca ttggtaacag
     7621 ctgaacctgc aagtccaaga ccgataacga tgatcatagg accgataatg attggtggca
     7681 agagtttatc aatccatttt gttcctacaa atcggatgct ggtagcaaca aggacataga
     7741 ccaaaccagt caagataacc cctgtttgag cagcagatac atcccccccc atttctttca
     7801 tagccagtga catagctgtg ataaaggcga atgaagaacc tagataaact ggaactttaa
     7861 aaccagtagc aagcatgtag atgagtgttc caacacctga agcaaaaagg gcaacagata
     7921 caggcattcc caaaatcaat ggtaccaaga tggtcgcacc aaacatggcg aaaacgtgtt
     7981 ggaaactaag gagaattcct tttccagccg aaggacgttg gtcaacgtct agtaacaaat
     8041 caacagttga ttcttgtttc atataaacct cacttttggg catcaaaaaa gagcccatta
     8101 ttttacagca ataggccctc agagtaagac ttctttaaaa tttagatttt aaagagtttt
     8161 aaatatttct gcgtcttcgt cacctcacgg gatgacatta aaaacctttg cttaggatag
     8221 tatagcagaa aaaaatgatt ttgtaaatca ttttttcccg agcctagaaa taaaagagcg
     8281 aggctgattt tgtaaacatt tgtttactaa aaaatttaaa gtgggcgttt atttggcata
     8341 ccagccttac gtggatattt atttggtgtt tcttttttct tttctaccac tgtgatatag
     8401 cgcggatctc tattcggtag ggcgtagctg agattgtctt cgaccttact aaaaaggaga
     8461 ttgagggcat tcttagcttc taataattcc tcaggcgcat tgctggcctt gagtgccaat
     8521 agttttccac caaccttaag gtagggaatg gtcaattcag ataggacctg catacgggca
     8581 accgcacgag ctgttacaaa atcatattga gcacggaagt tcttgtcttg ggcaaaatct
     8641 tcggcacgtc cgtggtagaa atgaactccg ttcaaatcca gttcttgagc caagagttgt
     8701 aggaagttga tgcgcttatt gagtgaatca ataatggtca catctaactc cggatagaga
     8761 attttcattg gtagactagg aaatcctgcc ccagccccga tatcaagaag tttgatagtt
     8821 tcattgggaa tcaaaccttg aagaatgggt gcaatcgaat cgtaaaaatg tttgagataa
     8881 acttcttcct tgtccgtaat cgccgtcaaa ttaatcttct cattccactc gaccaagagc
     8941 tcaaaataac gttcaaattg ttctttttgc tggtccgaaa gtggaagatt ctgctcggca
     9001 agcaagttgt aaaatgtttt tggtttcata gttatttcct tctttagtat catcttattt
     9061 taccacaatc atagaaaatt tgatatactg gtactaatct aaaagcagaa aggacttata
     9121 ttatgacttg gattattctt ggagttatcg ctcttattgt tatttttgtg attgttagct
     9181 ataacggttt ggttaaaaat cgtatgcaaa ccaaggaggc ttggagtcag attgatgttc
     9241 agttgaaacg tcgaaatgac ctcttgccga acttgattga gactgtaaaa ggttatgcca
     9301 aatatgaagg ttctaccctt gaaaaggtgg cagaactacg taaccaagtg gcggcagcga
     9361 cttcaccagc agaagctatg aaagccagtg atgccctcac tcgtcaggtt tcaggtattt
     9421 ttgcagttgc agaaagctat ccagatttga aagctagtgc taactttgtt aaattgcaag
     9481 aggagttgac aaacacagaa aataaaattt cttactctcg tcaactctat aacagtgttg
     9541 tcagcaacta caatgtaaaa ttagaaactt tcccgagcaa tattatcgct ggaatgtttg
     9601 gatttaaagc ggcagatttc cttcaaacac ctgaagagga aaagtcggtt cctaaagttg
     9661 attttagcgg tttaggtgac taagatgttg tttgatcaaa ttgcaagcaa taaacgaaaa
     9721 acctggattt tgttgctggt atttttccta ctcttagctc ttgttggtta tgcggttggt
     9781 tatctcttta taagatctgg acttggtggt ttggttatcg cactgattat cggctttatc
     9841 tacgctttgt ctatgatttt tcaatcgaca gagattgtca tgtccatgaa tggagcgcgt
     9901 gaggtggatg agcaaacggc accagacctc taccatgtag tggaagatat ggctctggtc
     9961 gcccagattc ctatgccccg tgttttcatc attgatgatc cagccttaaa tgcctttgcg
    10021 acaggttcta atcctcaaaa tgcggctgtt gctgcgactt caggtctact agctatcatg
    10081 aatcgtgaag aactagaagc tgttatggga catgaagtca gtcatattcg taattatgat
    10141 atccgtattt cgactattgc agttgccctt gctagtgcta tcaccatgct ttctagtatg
    10201 gcaggtcgta tgatgtggtg gggtggagca ggtcgcagac gaagtgatga tgaccgagat
    10261 ggaaatggtc ttgaaatcat tatgctagtg gtttccctac tagctattgt actggcacct
    10321 ctcgctgcaa ccttggttca gctcgctatt tctcgtcaga gggaatttct ggcagatgca
    10381 tctagtgtcg agctgactcg caatccccag ggaatgatta atgccctaga taagttggac
    10441 aatagcaaac ctatgagtcg ccacgtcgat gatgctagca gtgctcttta tatcaatgat
    10501 cccaagaaag gtggggggtt ccaaaaactc ttttataccc acccacctat ctcagaacgg
    10561 attgaacgtt taaaacagat gtaaaatgaa ggctggaaaa aagtctttaa aatctgaaaa
    10621 acgcataata tcaggtgtga aaacttgata ttatgcgttt tactatggga agatttactt
    10681 ctttttctcc taaaattgtg tttttgcccc acctatctgc tatgttgcaa atttataaat
    10741 cttctaaatt aactagcttc aaaccgtgtt ctgtcatgcg atagatgatc gagcagattt
    10801 cttttaagaa acgacggtca tgcgaaacag tgatgagacc gcctggatag gttgcaaaga
    10861 gttttctgat ttggggttga gaagtgggag aaaagtttcg tgtgggttca tccagcagga
    10921 gaaagtttgg tttgcgcagg actaaatcca aaagcaggag ttttccctgt tgtccgccag
    10981 ataaggagcg aatttgatgc tgcatttctg gataactgaa attgagactg gctaggtgag
    11041 attggatttt ctgaagttcc tctttttccc cagttttgct gatataagct attggtgata
    11101 aatccaattg cagttttttg tggtaatctt gtggcataaa accaagggaa atctctcttt
    11161 tatcattcag aagttgcttt aatttggcta acagagttga tttcccaaca ccatttggcc
    11221 cgataatacc gattttttct tgcccacgga cagttagttg tagtttttga accaaaactc
    11281 ggtcgtcaat ggacaaattt tctttttcca gttggactaa gactttagaa gctggtaatg
    11341 gttggatgtc tgaaaagaaa agttggattt gttcctcttc aagtggcttt tgagtcatgg
    11401 actgagctgt cttttcgtag cgtttttctt gtgagaggac agttttcatc tttttagcca
    11461 ataggcgacc ggcagtacta tctttggtag ctcgaagcgc agtttctaca ttttgcttaa
    11521 ctctccgatg tttttccatg gttttatcgt aggctctttg gttgttagca gcttgctgac
    11581 tttgtttggc aaaattagcc tttctctgct cactatagct atcatagtct aaatgctcta
    11641 ctagcgtttc cgcttcttta cggtgtttga ccagtcgcaa gtgaacaata gtgtctgctg
    11701 tttcagaaag aaagtcttca tcatgggaaa tgaaaataac ggtttgcctg gtcttttgaa
    11761 tctggccttt tagccaatca actgtctcaa ggtctaggtc atttgaaggt tcatctaaaa
    11821 atagaatctc aaagggtttg gctaactcat ggataagctg aattttcaaa gcttctcccc
    11881 ctgatagatt gccaatcttt tggtcacttg cgaaacgatt gctatcaaaa tgcaattcct
    11941 ccgccaaacg atagaggata ctgtagtcta aatcaataga atctaaaaag aagtagtcgt
    12001 gtaaagtttt ctttttcagc tcctcgggaa gtttttgagg aatgtaggcc agtgactgat
    12061 agtcagattg gatgtttccc ttgatagtga aatcagacaa agcttccccc attaaaattt
    12121 taagtaaggt tgatttgcca tttccttctt caccaataat agcaaccttt tccccgtctt
    12181 ggatggtcat gcttaggtca gatacaagat ctcgtaaatc tttgttttgt gtgatggtta
    12241 gatgattgat ttttatcata tgatttccct ttctagaatg ttcatagtgc ataacaaaaa
    12301 gctctacttt acctagtaga gcccaaagtc tatgtcaaaa aaactctatt aacatgccag
    12361 aaaaagagca taaaagagca cacaaaaaga gacaaaaaca agatctacta aataaaggtt
    12421 ctttatttga tagacttagt tgttcatgtc tcccttacct ccgagtatta gtatctttat
    12481 attatacctt taaggaaatt ttgtcaacag aaaactggaa attctatgct ctaaaatcca
    12541 aaaacaggtc catagagact tagtacgtgt ttgacatgct cgtaatggaa tttatcatag
    12601 tttcgccaag cagtgcgtgc ttgatcgttt agttttaggc tacccagtcc aatcagaaga
    12661 ctggtgggtt ggtaaaattt ctcaagatca attaagatac tgttgttcaa cttttccgtt
    12721 tttttgagct cttgcatagt agtgtttagc agctcttgta gtcgaatatc atctgccgta
    12781 ccctgtttac agttttggta gcttttattt aacttggata ggagttgaat gagtttcttg
    12841 tcttccatgt gagcatcctc cttgatctga tgtactcttt ccctatagta tagcactaaa
    12901 aaacaaaact gtcatagtaa atatgttaaa attctgaatt ttaattacaa agtttggtgt
    12961 gctgatttgt agccttttca tggtataatc aagagagtaa atctttatgg aggaagtatg
    13021 aaattaaata ttcaagaaat tcgtaagcag tctgaaggtt tgaactttga acaaacgtta
    13081 gatttagttg atgacctgcg tgcacgtaat caagaaattt tagatgtaaa agatatcctt
    13141 gcagttggga aagtacaata tgaagaccgt atgtatttct tagattatca actatcttat
    13201 accattgttc ttgcttcgag tcgcagtatg gagccagttg agttagttga atcttatcca
    13261 gtcacggaag ttttcatgga aggcgcaact aaccatctaa atcaagaaat tttagatgat
    13321 gacttggtct tgcccatcga aaatgaggcg cttgaccttg ctgagagtgt atcagacaat
    13381 atcttgctaa acattcctat caaggtcttg acggctgaag aagaagctgg tcaaggattt
    13441 gtctcaggaa atgactggca aatcatgaca gaggaagaat accaagctca acaagcagta
    13501 aagaaagaag aaaacagtcc ttttgctggc ttacaaggac tatttgacgg agatgaataa
    13561 tggtcttgtc aaaaaaacga gcacgaaagg tgctagaaga aatcattgcc ctcttcccag
    13621 atgccaagcc tagtcttgat tttaccaatc attttgaact cctggttgcg gtcatgttat
    13681 cagcccagac gacagatgca gcggtaaata aggccacacc aggtctcttt gttgcctttc
    13741 caacacccca agctatgtct gtagcgacag agagtgagat tgcttcacat atttctcgct
    13801 tgggattgta tcggaataaa gctaaattcc ttaaaaaatg tgcccaacag ttactagacg
    13861 attttgatgg tcaagtccct cagacacgtg aagaattgga gagtttggca ggtgttggtc
    13921 gcaagacagc caatgttgtc atgagtgtag gatttgggat tccagccttt gcagttgata
    13981 ctcatgtgga gcgtatttgc aaacaccacg atattgtcaa aaaatcagcg acgccacttg
    14041 aggtggaaaa gcgggtcatg gatatcttgc cgcctgagca gtggttagct gcccatcagg
    14101 ccatgattta ttttggaaga gccatttgcc atccaaaaaa tccagagtgt gaccagtacc
    14161 cacaattata tgattttagc aatttgtaag atgctacatt gaaacttttt gcttgatttt
    14221 aagcatgctt tgtgctatag taaatgataa ctaaatattc ctttaaacct gtcccgtgag
    14281 gcaggcaagg agcgcgataa agtagaaggg tgaagtctat actgtgtgca gtagggctcg
    14341 cttggctata aatttcaagt ctccttgttt aaggagactt ttctttttgg aggtttgtca
    14401 tgaaaacaaa agaagttgta gacgaattga ccgtcaaacg agcgattacg cgtattactt
    14461 atgagattat cgaacgcaac aaagatttga ataagatcgt cttggctggt attaaaactc
    14521 gtggtgtctt tattgcccac cgaatccaag aacgtttgaa gcagttagaa aatctttcag
    14581 ttcctgttgt ggaattggat actaaacctt tccgtgatga tgttaaaagt ggagaagata
    14641 cttctttggt ttctgtcgat gtgacagacc gcgaagttat cttggtggat gatgtgctct
    14701 atacaggtcg taccatccgt gctgctattg ataatattgt cggtcatggt cgtcctgcgc
    14761 gtgtgagttt agcagttcta gtcgatcgtg gacatagaga attgccaatc cgtccagatt
    14821 acgttggaaa aaatatccca accagtcgtt ctgaagaaat catcgtagag atgacagaac
    14881 ttgatgacca agacagagtt ctgattactg aagaagctta gaaagttaaa ggagtagcca
    14941 tgtcagaaaa tcaacaagca ttgaaccatg tggtgtccat ggaagacctc actgtcgatc
    15001 aagtaatgaa attgatcaag cgaggaattg agtttaaaaa tggagcccag cttccctatg
    15061 aagaccatcc gattgtttcc aatctcttct ttgaggattc tacacggaca cataagtcct
    15121 ttgaagtcgc agagattaaa cttggattgg aacgacttga ctttgatgtg aagactagtt
    15181 cggttaataa gggtgagaca ctttatgaca ccattttgac tctgtctgct ttaggagtgg
    15241 atgtctgtgt gattcgccat ccagaggtcg actactatag agaattgatt gctagtccaa
    15301 cgattacgac ttccatcatc aatggtggag atggttcggg ccaacaccct agccagagct
    15361 tgcttgattt gatgaccatt tatgaggaat ttggccactt tgagggtctt aaagttgcta
    15421 ttgcaggtga cttggaccac tcacgcgttg ccaaatccaa tatgcagatt ttgaaacgct
    15481 tgggatctga actctttttc gctggacctg aggaatggag aagtcaagag tttgcagact
    15541 atggacagtt tgtaaccatt gatgaaatca ttgatcaggt tgatgtcatg atgtttctcc
    15601 gtgtgcaaca cgaacgccat gatagtggag ctgtattttc aaaagaagac taccatgccc
    15661 aacatggctt gactcaagaa cgttacgatc gtttgaaaga aacagcaatc ctcatgcacc
    15721 cagctccaat caatcgtgat gtagaaatag cagaccactt ggttgaagca ccaaaatcac
    15781 ggattgtcca acaaatgacc aatggtgtct ttgttcgaat ggcaatctta gaatccgtac
    15841 tagcgagtag aaacgccaac taaaacacaa gacgttaaaa gacgccagtg agcgtccacg
    15901 agaggtgaaa atatgacaaa aagaattcta gtattagaag atggcacagt ttttgaaggc
    15961 aaggccttcg gagcagatat tgatgtaaca ggcgaaatcg tctttaatac agggatgacc
    16021 ggctaccaag aatccattac agaccagtct tataatggac aaatcttgac ctttacttat
    16081 cctttggtag gaaattatgg tattaatcgt gatgattatg aatccattat tccaacttgt
    16141 aagggagtcg ttgtttttga agaagcgcgt agagctagca actggcgcaa ccaaatgacc
    16201 ttggatgaat ttttgaaagc caagaaaatt ccagggattt caggaattga tacgcgtgct
    16261 cttaccaaga ttatccgtaa gcatggtact atgcgtgcaa ccttgaccca tgttggggac
    16321 agtatggacc atgtgacgga ccagctccaa gcaacagttt taccgacaga caatatcaaa
    16381 caggtttcta ctaaaacgtc ttatccagct ccaggagttg gtttgagcgt ggtgctagtg
    16441 gactttggtc tcaagcactc aatcctacgt gaactttcta aacgcaactg taacgtgaca
    16501 gttgttcctt attcgacaac ggctgaagaa attctccatc tcaatcctga cggagttatg
    16561 ttgtcaaatg gtccaggtaa cccagaagac gttccacaag cactcgacat gattcgcggt
    16621 gtacaaggaa aaattccaat ctttggtatt tgtatgggac accaactctt tgcaatggca
    16681 aacggggcta agacctataa gatgaagttt ggccaccgtg gattcaacca tgcggtacgt
    16741 gaaatcgcaa caggacgagt agacttcacc agccagaacc atggttatgc agtcagccgc
    16801 gaggatttgc cagagcactt gattattacc cacgaagaaa tcaatgacaa gtcagtggaa
    16861 ggtgtgcgcc acagatacca acctgctttc tctgttcaat accacccaga tgcagctcct
    16921 gggccacacg acgctagcta cctatttgac gagtttatcg agatgatgga agtttttaaa
    16981 caatcaaact aagaacgagt aaaagctcgt cggagaattt atgtaactga acacggacgg
    17041 aaagctcaga atgtagagaa accaacttgg attgtcaatc cttccttgat ttcctaaaat
    17101 tcaatcgctt tctattcgtc ctttgtatct tatttaatgt cgccctgaga ggcgacgaat
    17161 agaaaggaga agggagctcc gtatttgctg aactgaatac gggctacgga cggtgctaaa
    17221 aagatagtta ttcctagaac tgacagttct tcgtcataac tcctatttta gctttgtccg
    17281 cttgacaccc ttaatatctt atatatgcct aaacgtactg atattcaaaa aattatggtg
    17341 attggttctg gtccgattat tattggtcag gctgctgagt ttgactacgc tgggactcag
    17401 gcttgcttgt cgttgaaaga ggaaggttat gaggttgttt tggttaactc aaatcctgca
    17461 accatcatga cggacaagga aattgctgac aaggtttaca ttgaaccgat tacacttgag
    17521 tttgtgacac gtattcttcg taaggaaggt ccagatgcct tgctaccaac actcggtggt
    17581 cagacaggtc tcaacatggc catggaatta tctaaaaatg gtatcctaga cgagcttggc
    17641 gttgaacttc tgggtaccaa attatctgcc attgaccaag cggaggaccg tgatctcttt
    17701 aaacaattga tggaagagct tgaacaaccc attccagaat ctgaaattgt taacacagta
    17761 gaagaagcag ttgcctttgc agcgacaatt ggctacccag tcatcgttcg tccagccttt
    17821 acccttggtg gtactggtgg tggtatgtgt gccaacgaga aagaattgcg tgaaatcact
    17881 gaaaatggtt tgaaattgtc acctgttacc caatgtttga ttgagcgttc gattgccggt
    17941 ttcaaggaaa ttgaatacga agtgatgcgt gactcagctg acaatgcctt ggttgtttgt
    18001 aacatggaaa actttgaccc agttgggatt cacacagggg attccattgt atttgcccct
    18061 gcgcaaacca tgtcagacta tgaaaaccaa atgcttcgtg atgcgagctt gagcattatt
    18121 cgtgccctca agattgaagg tggatgtaat gttcagctag ctcttgatcc taacagcttc
    18181 aagtattatg ttatcgaagt aaaccctcgt gtatcacgtt cgtcagccct tgcttctaaa
    18241 gcgacaggat atccgattgc taagttggct gccaagattg cagtaggttt gaccttggat
    18301 gaggttataa acccagttac aggttcaacc tatgccatgt ttgaaccagc ccttgactac
    18361 gtagttgcta agattccacg tttcccattt gacaagtttg aaaagggtga acgccgtctc
    18421 ggtacacaaa tgaaggcgac tggagaagtc atggcaatcg gtcgtaacat cgaggaatct
    18481 cttctcaagg cctgccgctc ccttgaaatt ggggtgcacc acaatgaaat acctgaactt
    18541 gcagcagttt ctgatgatac cttgattgaa aaggttgtca aagcccaaga tgaccgtctc
    18601 ttctacgtat cagaagccat tcgtcgtggc tacacacctg aagaaattgc agagcttaca
    18661 aaaattgata tcttctatct tgataaactc ttgcatatct ttgaaattga gcaagaattg
    18721 ggtgctcatc cacaagatct agaagttttg aaaacagcca aacttaatgg tttttcagac
    18781 cgtaagattg ctgaactctg gggaacgact gatgataaag ttcgccaact tcgcttggaa
    18841 aacaagattg ttccagttta taagatggta gacacttgtg cggcagagtt cgattccgaa
    18901 acgccatatt tctattcaac ctatggttgg gaaaatgaat ctatcaaatc tgataaagaa
    18961 tctgtacttg tcctaggttc aggtccaatc cgtatcggtc aaggggttga gtttgactac
    19021 gcaaccgttc actctgttaa ggctatccag gcagctggtt atgaagctat catcatgaac
    19081 tcaaacccag agaccgtttc aacagacttc tctgtatcag ataagcttta ctttgagcca
    19141 ttgacattcg aagatgttat gaacgtcatt gacttggaac aaccaaaggg agttatcgtt
    19201 cagttcggtg gtcaaacagc catcaacctt gcagagccat tggcaaaagc aggtgtgacc
    19261 atccttggta cacaggttgc tgacctagac cgtgccgaag accgtgacct cttcgagcaa
    19321 gctctcaaag aattggatat tccacagcca ccaggacaaa cggctaccaa tgaagaagaa
    19381 gcagctcttg cagctcgtaa gattggcttc ccagttcttg tccgcccatc ttatgtactt
    19441 ggtggacgtg ccatggaaat cgttgaaaac gaagaagacc ttcgttctta catgcgaact
    19501 gctgttaagg ctagtccaga ccatccagtt cttgtcgact cttatatcgt tgggcaagag
    19561 tgcgaagttg atgccatttc agatggtaaa aatgtactca ttcctggtat catggagcat
    19621 atcgaacgtg ctggtgtcca ctcaggtgac tcaatggctg tttacccacc acaaaccttg
    19681 tcgcaaaagg ttcaggagac catcgcagac tacaccaaac gcttagcaat cggtcttcac
    19741 tgccttggaa tgatgaacat ccagtttgtt atcaaggatg aaaaagtcta cgttattgag
    19801 gtcaatccac gtgccagccg taccgttcca ttcctatcta aggtaaccaa tattcctatg
    19861 gctcaagtag cgaccaagct cattcttggt caaagtttat cagaacttgg ctaccaaaat
    19921 ggactttacc ctgaaagcac ccgtgttcat atcaaggcac ctgtcttctc ctttaccaaa
    19981 ctagctaagg tagacagctt gctcggtcct gaaatgaagt caacaggtga agttatgggt
    20041 tctgatgcta ctttggaaaa agctctctat aaagcctttg aagcttctta tctacacttg
    20101 ccaacttttg gtaatgtcgt ctttaccatc gctgatgatg ccaaagaaga agccttgaac
    20161 ttggctcgtc gtttccaaaa cattggttat ggaatccttg cgacagaagg gacagcagcc
    20221 ttctttgcta gtcatggctt gcaagcccaa cctgttggta agattggtga tgacgataag
    20281 gatatcccaa gttttgtacg caaaggaaga attcaagcta tcattaacac agtaggaaca
    20341 aaacgaactg ctgacgaaga tggtgagcaa attcgtcgtt cagccattga acacggggtg
    20401 cccctcttca cagcgctaga tacagctaat gccatgctta aggtacttga aagtcgtagc
    20461 tttgttacag aagcgattta aatagaaaaa taagatatag cagactgaat ttagaacagt
    20521 acacagggat
//
LOCUS       DAVKQB010000041        19932 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKQB010000041 DAVKQB010000000
VERSION     DAVKQB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.041"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..752)
                     /locus_tag="VW528_001529"
     CDS             complement(<1..752)
                     /locus_tag="VW528_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9912337.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNTPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQI
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHAQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(749..2074)
                     /locus_tag="VW528_001530"
     CDS             complement(749..2074)
                     /locus_tag="VW528_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9912338.1"
                     /translation="MDFFLLVDLILYFLIISHSYRLICKDQINRKELFFFGAYTLLTE
                     IVLDFPFYLLYLDGLGIATFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYYGLFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRVYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKRKFE"
     gene            complement(2087..2227)
                     /gene="comC"
                     /locus_tag="VW528_001531"
     CDS             complement(2087..2227)
                     /gene="comC"
                     /locus_tag="VW528_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9912339.1"
                     /translation="MKNTVKLEQFVALKEKDLQNIQGGEMRKMNEKSFNIFNFFNFFR
                     RR"
     gene            complement(2392..2465)
                     /locus_tag="VW528_001532"
     tRNA            complement(2392..2465)
                     /locus_tag="VW528_001532"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2429..2431),aa:Arg,seq:tct)
     gene            complement(2509..2988)
                     /gene="rlmH"
                     /locus_tag="VW528_001533"
     CDS             complement(2509..2988)
                     /gene="rlmH"
                     /locus_tag="VW528_001533"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9912340.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            3171..4352
                     /locus_tag="VW528_001534"
     CDS             3171..4352
                     /locus_tag="VW528_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9912341.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            4410..5168
                     /locus_tag="VW528_001535"
     CDS             4410..5168
                     /locus_tag="VW528_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9912342.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNYFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIIYFSNQEEYSRIINSLK"
     gene            5381..6742
                     /gene="dnaA"
                     /locus_tag="VW528_001536"
     CDS             5381..6742
                     /gene="dnaA"
                     /locus_tag="VW528_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9912343.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            6901..8037
                     /gene="dnaN"
                     /locus_tag="VW528_001537"
     CDS             6901..8037
                     /gene="dnaN"
                     /locus_tag="VW528_001537"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9912344.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTIKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            8102..8296
                     /locus_tag="VW528_001538"
     CDS             8102..8296
                     /locus_tag="VW528_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9912345.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            8380..9495
                     /gene="ychF"
                     /locus_tag="VW528_001539"
     CDS             8380..9495
                     /gene="ychF"
                     /locus_tag="VW528_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9912346.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLIESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYESEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            9566..10135
                     /locus_tag="VW528_001540"
     CDS             9566..10135
                     /locus_tag="VW528_001540"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9912347.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     KADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            10136..13645
                     /gene="mfd"
                     /locus_tag="VW528_001541"
     CDS             10136..13645
                     /gene="mfd"
                     /locus_tag="VW528_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9912348.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKELMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            13703..13969
                     /locus_tag="VW528_001542"
     CDS             13703..13969
                     /locus_tag="VW528_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9912349.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            13962..14330
                     /locus_tag="VW528_001543"
     CDS             13962..14330
                     /locus_tag="VW528_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9912350.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGVVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            14450..15718
                     /locus_tag="VW528_001544"
     CDS             14450..15718
                     /locus_tag="VW528_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9912351.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKISFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            15715..16992
                     /gene="tilS"
                     /locus_tag="VW528_001545"
     CDS             15715..16992
                     /gene="tilS"
                     /locus_tag="VW528_001545"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9912352.1"
                     /translation="MREQDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNIDVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            16996..17538
                     /gene="hpt"
                     /locus_tag="VW528_001546"
     CDS             16996..17538
                     /gene="hpt"
                     /locus_tag="VW528_001546"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9912353.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            17554..19512
                     /gene="ftsH"
                     /locus_tag="VW528_001547"
     CDS             17554..19512
                     /gene="ftsH"
                     /locus_tag="VW528_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9912354.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            19634..>19932
                     /locus_tag="VW528_001548"
     CDS             19634..>19932
                     /locus_tag="VW528_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9912355.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRKYDKEPYEEVGELSHRISEGG"
BASE COUNT         6888 a         3183 c         3758 g         6103 t
ORIGIN      
        1 cacttttgag attttttctc taaaatatct tttaattttc taatttttaa tcttgaaata
       61 ggacaacgat ggtcttcata gaaaacaatt tctaagtttt ttcgatcaat ttctctgata
      121 ttgcctatat ttaccaaaaa tgacttgtga ggagaataaa atcgctgagc atgtttgtcc
      181 ttttcctgaa tatctgtcat ggtaccataa aactcttttg caaaattctt accaataatg
      241 cgcaatttat gagagactcc tgtcgtttca atatacaaaa tatcatggta aggaattttt
      301 aaatcatttc ccttgtaatt gtagtcgaaa taatctacaa catcttcatt ttcaagtaac
      361 atactcttcg tgtagaagat attttgctca attcgcttct taaacatctc atcgttgata
      421 tccttatcaa caaaatctag ggctgatatc tggtatttat aggtcagagt cgcaaactct
      481 gatcgactgg tgataaagac gataatagcg taaggattgt aatgacgaat gagttgagca
      541 acttcaaatc cctttttctc aattccatga atatcgatat ctaggaaata aagttgattt
      601 acttcatcat tttcaatgta ttcttcaaat tcacggactt ttcccgttgt cttgtatgat
      661 attggagtat tcgattcttt cgaaatttca tccaatattc tctctagtct cacttgatgt
      721 tcaataacat cttctaaaat taaaactttc attcaaattt cctcttaaat ctaatgattt
      781 gtctaaatgt actgccttcc atctctgttt ctaaaataat gttgttgtac ttatctagta
      841 gttctttcac attatttaat ccgactccgc gatttcttcc cttagtggag aatcctaagg
      901 caaatagatc tcctgaagga gtcatcgtca ttttacatga attctgaatc acaataactg
      961 tttcagtttc catcttaata actgctactt ccatctgctt tttatagcta tcagccgatc
     1021 cttcgacagc attgttcaat aaaacgctca tgatacgcac taaatccaat agttcaattg
     1081 gaagcttggt aatcgtatct tttacttcca gtgtaaactc tacaccatta tttcgagcat
     1141 agacaattga ctgagcaacc aaacttcgta aagctgaatc ttctatgttg ttcaaatcaa
     1201 agtaagtgta cttatctgaa cgtagtttat gatttgcttt gaccaaaact tcattgtaaa
     1261 ctctgtcaat ttcctgtaaa ttaccactgt caattgccat ctgcatgctg acaagcattc
     1321 cagcataatc atgtcgaaaa ccacggattt cattatacag accgacaatt tcatctgtgt
     1381 aattctgtaa atgtttctgt tcaaatttct tctgcttcaa agcaatctct ttctccattt
     1441 gaactttatg agaattcatt gcaaagaagg tcaaaaggag agagataaag acaatagatg
     1501 acaaaatact tccaaaacta ttcaaatgtt taatcgtact taccatatct gaaacgaaag
     1561 atacaatatg tagcaatagt aaagcaaaaa atactttttt caagaaagga taaaggtagt
     1621 ccttgtcaaa ataggctagt tccaaatgga aataataaat gatttttaat gtaacaaaat
     1681 aggtcaacac cgtcacaacg aaaaagaata ggccataata ctgtaaaaca aaattgtctc
     1741 ctgttataga agagaaagtt acggacagaa agttatgagt gctctcatat aaaagagata
     1801 gtagtaaact tagaaatagt cctctatccc tctcatactg tttcatccat cgaaaatagg
     1861 aatataggcc caaaggaaat aaaaatgttg caatccctaa cccatctaaa tatagaagat
     1921 agaagggaaa atcaagtact atttctgtta gtaatgtata agcaccaaaa aagaataatt
     1981 cttttctatt tatttgatct ttacaaatta aacggtaact atgactaata attaaaaaat
     2041 ataatatcaa atctactaat aagaaaaaat ccattctgct actcctttat cttcttctaa
     2101 agaaattaaa gaaattaaag atattaaagg acttttcatt cattttcctc atctccccac
     2161 cttgaatatt ttgcaagtct ttttccttca aagctacaaa ctgttccaat ttaactgtgt
     2221 ttttcataat aaaatctcct aaaatgtttt ttcttgtaag ctaacttaca aaaaccatta
     2281 tacaaaatgg aatttcgttt tagataaaat tctctcaact gtcatttttt tctcccaaag
     2341 tgtacttttt taagaaaaaa gccgggaaaa ttcccagctt tgctattata ttgatcccag
     2401 caggattcga acctgcgacc gttcgcttag aaggcgaatg ctctatccag ctgagctatg
     2461 agacctaaca caattattct accaaaaatt caattaaaag tcaattttct atttatggta
     2521 ggggaatccc tgctgaatcg taaaagcgcg atagatttgt tcaacaagaa ctagtctcat
     2581 taactgatga ggcaaggtta ggcgaccaaa actgacagaa agattggctc tattttttac
     2641 agatgatgat aatcctaaac ttcccccaat aataaaagta agagtagaaa atccttttat
     2701 agaagtttct tctaactgct tactaaattc ttctgagaag aaagttttcc cttcaatggc
     2761 taacacaata acgaaatcac ggtcagcaat ttttgataaa attctctgac cttctatttc
     2821 taaaatcttt tgattttctg attcactggc cttatctggt gttttttcat ctgataactc
     2881 aatcatttca aacttagcaa atctagaaat tcgttttgaa tactctgcga taccatcttt
     2941 taaatacttt tctttcagtt tcccaactgt tacaacttta attttcatga ctctattcta
     3001 acatattctc tattttttca catcttattc acaaaataaa aaatagattt caattaagaa
     3061 aatcacagtt tcaaaagagt tatccacagt ttgtgtaaaa cttttgtgtt taagttataa
     3121 ttaagctagt cagtttatac tttcagtaat tcaaacatat ggaggcaaat atgaaacatc
     3181 taaaaacatt ttacaaaaaa tggtttcaat tattagtcgt tatcgtcatt agctttttta
     3241 gtggagcctt gggtagtttt tcaataactc aactaactca aaaaagtagt gtaaacaact
     3301 ctaacaacaa tagtactatt acacaaactg cctataagaa cgaaaattca acaacacagg
     3361 ctgttaacaa agtaaaagat gctgttgttt ctgttattac ttattcggca aacagacaaa
     3421 atagcgtatt tggcaatgat gatactgaca cagattctca gcgaatctct agtgaaggat
     3481 ctggagttat ttataaaaag aatgataaag aagcttacat cgtcaccaac aatcacgtta
     3541 taaatggcgc cagcaaagta gatattcgat tgtcagatgg gactaaagta cctggagaaa
     3601 ttgtcggagc tgacactttc tctgatattg ctgtcgtcaa aatctcttca gaaaaagtga
     3661 caacagtagc tgagtttggt gattctagta agttaactgt aggagaaact gctattgcca
     3721 tcggtagccc gttaggttct gaatatgcaa atactgtcac tcaaggtatc gtatccagtc
     3781 tcaatagaaa tgtatcctta agatcggaag atggacaagc tatttctaca aaagccatcc
     3841 aaactgatac tgctattaac ccaggtaact ctggcggccc actgatcaat attcaagggc
     3901 aggttatcgg aattacctca agtaaaattg ctacaaatgg aggaacatct gtagaaggtc
     3961 ttggtttcgc aattcctgca aatgatgcta tcaatattat tgaacagtta gaaaaaaacg
     4021 gaaaagtgac gcgtccagct ttgggaatcc agatggttaa tttatctaat gtgagtacaa
     4081 gcgacatcag aagactcaat attccaagta atgttacatc tggtgtagtt gttcgttcgg
     4141 tacaaagtaa tatgcctgcc aatggtcacc ttgaaaaata cgatgtaatt acaaaagtag
     4201 atgacaaaga gattgcttca tcaacagact tacaaagtgc tctttacaac cattctatcg
     4261 gagacaccat taagataacc tactatcgta acgggaaaga agaaactacc tctatcaaac
     4321 ttaacaagag ttcaggtgat ttagaatctt aattgacatc tatgtaaaga aagctttaca
     4381 taagagaaaa gatgtgttag tgtagaatca tggaaaaatt tgaaatgatt tctatcacag
     4441 atatacaaaa aaatccctat caaccccgaa aagaatttga tagagaaaag ctagatgaac
     4501 tagcacagtc tatcaaagaa aatggggtca ttcaaccgat tattgttcgt caatctcctg
     4561 ttattggtta tgaaatcctt gcaggagaga gacgctatcg ggcttcactt ttagctggtc
     4621 tacggtctat cccagctgtt gttaaacaga tttcagacca agagatgatg gtccagtcca
     4681 ttattgaaaa tttacagaga gaaaatttaa acccaataga agaagcacgc gcctatgaat
     4741 ctctcgtaga gaaaggattc acccatgctg aaattgcaga taagatgggc aagtctcgtc
     4801 catatatcag caactccatt cgtttacttt ccttgccaga acagattctc tcagaagtag
     4861 aaaatggcaa actatcacaa gcccatgcgc gttccctagt tgggttaaat aaggaacaac
     4921 aagactattt ctttcaacgg attatagaag aagatatttc tgtaaggaag ttagaagctc
     4981 ttctgacaga gaaaaaacaa aagaaacagc aaaaaactaa ttatttcata caaaatgaag
     5041 aaaaacagtt aagaaaacta ctcggattag atgtagaaat taaactatct aaaaaagaca
     5101 gtggaaaaat cattatttat ttttcaaatc aagaagaata tagtagaatt atcaacagcc
     5161 tgaaataagg ctgttctttt atttttttat ctcacaaggt tatccactat gtttttcgat
     5221 aaaaagctta ataaatcaat aatttcttct tttatcccca acctgtggat aaagtttagt
     5281 aacattgtgg attatttttc acagcttgtg gaaaattctt gctatctatg gtaaaatatc
     5341 tctagtatta aacttttaaa tagtaaagga ggaaaaagga ttgaaagaaa aacaattttg
     5401 gaatcgtata ttagaatttg cacaagaaag actgactcga tccatgtatg atttctatgc
     5461 tattcaagct gaactcatca aggtagagga aaatgttgcc actatatttc tacctcgctc
     5521 tgaaatggaa atggtctggg aaaaacaact aaaagatatt attgtagtag ctggttttga
     5581 aatttatgac gctgaaataa ctccccacta tattttcacc aaacctcaag atacgactag
     5641 ctcacaagtt gaagaagcta caaatttaac tctttatgac tatagtccaa agttagtatc
     5701 tattccttat tcagatacgg gattaaaaga aaagtatacc tttgataact ttattcaagg
     5761 ggatggaaat gtttgggctg tgtcagccgc tttagctgtc tctgaagatt tggctctgac
     5821 ctataaccct ctttttatct atggaggacc aggccttggt aagactcact tattaaacgc
     5881 tattggaaat gaaattctaa aaaatattcc taatgcgcgt gttaaatata tccctgccga
     5941 aagctttatt aatgactttc ttgatcacct aagacttggg gaaatggaaa agtttaaaaa
     6001 gacctatcgt agtcttgatc ttttgttaat cgatgatatc cagtcactca gcggaaaaaa
     6061 agtcgcaact caggaagaat ttttcaatac ctttaacgcc cttcatgaca agcaaaaaca
     6121 gattgtccta acgagtgatc gtagtccaaa acatctagaa gggctcgagg agaggcttgt
     6181 cacgcgtttt agttggggat tgacacaaac tatcacaccc cctgactttg aaacacgtat
     6241 tgccatttta caaagtaaaa cggaacattt aggctacaat ttccaaagtg atactctaga
     6301 atacctagct gggcaatttg attcaaatgt tcgagatctt gagggagcca tcaacgacat
     6361 cactttaatt gccagagtaa aaaaaatcaa ggatatcact attgatattg ctgcagaagc
     6421 cattagagcc cgcaaacaag atgttagcca aatgctcgtc atcccaattg ataaaatcca
     6481 aactgaagtt ggtaactttt atggtgttag tatcaaagaa atgaagggaa gtagacgcct
     6541 tcaaaatatt gttttggccc gtcaagtagc catgtattta tctagagaac taacagataa
     6601 tagtcttcca aaaattggga aggaatttgg gggaaaagat cataccacag tcattcatgc
     6661 ccatgccaaa ataaaatctt tgattgatca agacgataat ttacgtttag aaattgaatc
     6721 aatcaaaaag aaaatcaaat aatttgtgga taacttttag ttttttatct tttttatcca
     6781 cattttttaa acaagctaaa aaacttgata ggacttgttt aaaggctgtt ttccacagat
     6841 ttcacagact ctattattac tattatcttt ctaatactaa aaataaataa aggagaatcc
     6901 atgattcatt tttcaattaa taaaaattta tttctacaag cattaaatac tactaagaga
     6961 gctattagtt ctaaaaatgc cattcctatt ttatcaacaa taaaaattga cgtgaccaat
     7021 gaaggtatta ctttaattgg ttcaaatggt caaatttcaa ttgaaaattt tatttctcaa
     7081 aaaaatgaag atgctggttt gttaattact tctttaggtt cgatccttct tgaagcttct
     7141 ttctttatca atgtagtatc tagtttacct gatgtaactc ttgattttaa agaaattgaa
     7201 caaaatcaaa ttgttttaac cagtggcaaa tcagaaatta ccctaaaagg aaaagatagc
     7261 gaacaatatc cacgaatcca agaaatttca gcaagcactc ctttaatact tgaaacaaaa
     7321 ttactcaaga aaattattaa tgaaacagcc tttgctgcaa gtacacaaga gagtcgtccg
     7381 attttaacag gtgtccactt cgtattgagt caacacaaag agttaaaaac agttgcaaca
     7441 gactctcatc gcctaagcca gaaaaaattg actcttgaaa aaaatagtga tgattttgat
     7501 gtcgtaattc ctagccgttc tctacgcgaa ttttcagcgg tatttacaga tgatatcgaa
     7561 actgtagaga ttttctttgc caataaccaa atcctcttta gaagcgaaaa tattagcttc
     7621 tatactcgtc tcctagaagg aaactatcct gatacagatc gcttgattcc aacagacttt
     7681 aacactacta ttacttttaa tgtggtaaac ttacgccagt caatggagcg tgcccgtctt
     7741 ttatcaagtg cgactcaaaa tggtactgtg aaacttgaaa ttaaggatgg ggttgttagc
     7801 gcccatgttc actctccaga agttggtaaa gtaaacgaag aaatcgatac tgatcaggtt
     7861 actggtgaag atttgaccat tagtttcaac ccaacttact tgattgattc tcttaaagct
     7921 ttaaatagcg aaaaggtgac cattagcttt atctcagctg ttcgtccatt tactcttgtg
     7981 ccagcagata ctgacgaaga cttcatgcag ctcattacac cagttcgtac aaattaagtg
     8041 aaagaggttg agcctggctc gcctctttta tgatataatc gaaaaagaaa aggagagtag
     8101 tatgtatcaa gttggaaatt ttgttgagat gaaaaaatca cacgcttgta caatcaagtc
     8161 gactggtaaa aaggctaatc gttgggaaat tacacgtgta ggagcagata tcaaaataaa
     8221 atgcagtaat tgtgagcatg ttgtcatgat ggggcgatat gattttgagc gaaaaatgaa
     8281 taaaattatt gactgagaac ccttagttag agggttagca ctttatccct ttttgtgtta
     8341 taatattagg gattgaaatg aaaacggaga atgagaaata tggctttgac agcaggtatc
     8401 gttggtttgc caaacgttgg taaatcaaca ctatttaatg caattacaaa agcaggagca
     8461 gaggcagcaa actacccatt tgcgactatt gatccaaatg ttggaatggt ggaagttcca
     8521 gatgaacgcc tacaaaaact aactgaaatg ataactccta aaaagacagt tcccacaaca
     8581 tttgaattta cggatattgc agggattgta aaaggagctt caaaaggaga agggctaggg
     8641 aataaattct tggccaatat tcgtgaagta gatgcgattg ttcacgtagt tcgtgctttt
     8701 gatgatgaaa atgtgatgcg cgagcaagga cgtgaagacg cctttgtgga tccacttgca
     8761 gatattgaca ccattaacct agaattgatt cttgcagact tagaatcagt caataaacga
     8821 tatgcgcgtg tagaaaagat ggcacgtacg caaaaagata aagaatcagt agcagaattc
     8881 aatgttcttc aaaagattaa accagtccta gaagacggga aatcagctcg taccattgaa
     8941 tttacagatg aggaacaaaa ggttgtcaaa ggtcttttcc ttttgacgac taaaccagtt
     9001 ctttatgtag ctaatgtgga cgaggatgtg gtttcagaac ctgactctat cgactatgtc
     9061 aaacaaattc gtgaatttgc agcgacagaa aatgctgaag tagtcgttat ttctgcgcgt
     9121 gctgaggaag aaatttctga attggatgat gaagataaaa aagagtttct tgaagccatt
     9181 ggtttgatag aatcaggtgt agataagttg acgcgtgcag cttaccactt gcttggattg
     9241 ggaacttact tcacagctgg tgaaaaagaa gttcgcgctt ggactttcaa acgtggtatg
     9301 aaggctcctc aagcagctgg tattatccac tcagactttg aaaaaggctt tattcgtgca
     9361 gtaaccatgt catatgagga tctagtgaaa tacgaatctg aaaaggccgt aaaagaagct
     9421 ggacgcttgc gtgaagaagg aaaagaatat atcgttcaag atggcgatat catggaattc
     9481 cgctttaatg tctaaaaatt aataaatggt gtcaattagg ttggaaaaaa attccaaccc
     9541 ttttggcttt tgaaaggaaa aataaatgac caaattactt gtaggcttgg gaaatccagg
     9601 ggataaatat tttgaaacaa aacacaatgt tggttttatg ttgattgatc aactagcgaa
     9661 gaaacagaat gtcactttta cacacgataa gatatttaaa gctgacctag catccttttt
     9721 cctaaatgga gaaaaaattt atctggttaa accaacgacc tttatgaatg aaagtggaaa
     9781 agcagttcat gctttattaa cttactatgg tttggatatt gacgatttac ttatcattta
     9841 cgatgatctt gacatggaag ttgggaaaat tcgtttaaga gcaaaaggct cagcaggtgg
     9901 tcataatggt atcaagtcta ttattcaaca tataggaact caggtcttta accgtgttaa
     9961 gattggaatt ggaagaccta aaaatggtat gtcagttgtt catcatgttt tgagtaagtt
    10021 tgacagggat gattatatcg gtattttaca gtctgttgac aaagttgacg attctgtaaa
    10081 ctactattta caagagaaaa attttgagaa aacaatgcag aggtataacg gataaatggt
    10141 gaccttatta gatttattct cagaaaatga tcagattaaa aaatggcatc aaaatttaac
    10201 tgataagaaa agacaactaa tacttggttt atcaacatct actaaggctc ttgcaattgc
    10261 aagcagttta gaaaaagaag ataggattgt gttattgacg tcaacttatg gagaagcaga
    10321 aggacttgtt agtgatctta tatctatctt gggtgaggaa ctcgtctatc catttttggt
    10381 agatgatgct cctatggtgg agtttttgat gtcttcacag gaaaaaatta tttcacgggt
    10441 tgaagccttg cgttttttga ctgattcatc taagaaaggg attttagttt gtaatatcgc
    10501 agcaagtcga ttgattttac cgtctcccaa tgcattcaaa gatagtattg taaaaatctc
    10561 agttggtgaa gaatatgatc aacacgcgtt tatccatcag ttaaaggaaa atggctatcg
    10621 aaaagttact caagtacaaa ctcagggcga atttagtctt cgaggagata ttttagatat
    10681 ttttgaaata tcccagttag aaccttgtcg aattgagttt tttggtgatg aaattgatgg
    10741 tatcaggtca tttgaagtag aaacacaatt atcgaaagaa aataagacag aactcactat
    10801 ctttccagct agtgatatgc ttttgagaga aaaggattat caacgaggac agtcagcttt
    10861 agaaaaacaa atttcaaaaa ctttatcacc tattttgaaa tcatacctag aagaaattct
    10921 ttcaagtttt caccaaaaac aaagtcatgc agactctcgg aagtttttat ctttgtgcta
    10981 tgataagaca tggactgtct ttgattatat tgaaaaagat actccaatat tctttgatga
    11041 ttatcaaaaa ttgatgaatc agtatgaagt ctttgaaaga gacttagcgc agtactttac
    11101 agaagaatta cagaatagta aagcattttc tgatatgcag tatttttctg atattgaaca
    11161 aatctataaa aaacaaagtc cagtgacctt tttctctaat cttcaaaagg gtttaggaaa
    11221 tctcaaattt gacaaaattt atcaattcaa tcaatatcct atgcaggaat ttttcaatca
    11281 gttttctttt ctaaaagaag aaattgaacg atataaaaaa atggattaca ccattattct
    11341 gcagtctagc aattcaatgg gaagtaaaac attggaggat atgttagagg aatatcagat
    11401 taaattggat tctagagata agacaaatat ctgtaaagaa tctgtaaact taatagaggg
    11461 taatctcaga catggttttc attttgtaga tgaaaagatt ttattgataa ctgaacatga
    11521 gatttttcaa aagaaattaa agcgtcgttt tcgaagacaa catgtttcaa atgcagagag
    11581 attaaaagat tacaatgaac ttgaaaaagg ggactatgtt gtccatcata tccatgggat
    11641 tggtcaatat ctaggaattg aaaccattga aatcaaggga attcatcgcg attatgtcag
    11701 tgtccaatac caaaatggtg atcaaatttc tatccccgtg gaacagattc atctactgtc
    11761 caaatatatt tcaagtgatg gtaaagctcc aaaactcaat aaattaaatg acggtcattt
    11821 taaaaaggcc aagcaaaagg ttaagaacca ggtagaggat atagctgatg atttaatcaa
    11881 actctactct gaacgtagtc agttgaaggg ttttgctttc tcagctgatg atgatgatca
    11941 agatgccttt gatgatgctt tcccttatgt tgaaacggat gatcaacttc gtagtattga
    12001 ggaaatcaaa agggatatgc aggcttctca gccaatggat cgacttttag ttggggatgt
    12061 tggttttgga aagactgaag ttgctatgcg tgcagccttt aaagcagtca atgatcacaa
    12121 acaggttgtc attctagttc cgacgacggt tttagcgcaa cagcactata cgaattttaa
    12181 ggaacgattc caaaattttg cagttaatgt tgatgtgttg agtcgcttta gaagtaaaaa
    12241 agagcagact gcaacacttg aaaaattgaa aaacggtcaa gtcgatattt tgattggaac
    12301 acatcgtgtt ttgtcaaaag atgttgtgtt tgctgatttg ggcttgatga ttattgatga
    12361 ggaacagcga tttggtgtca agcataagga aactttgaaa gaactgaaga aacaagtgga
    12421 tgtcctaacc ttgaccgcta cgccaatccc tcgtaccctc catatgtcta tgctgggaat
    12481 cagagatttg tctgttattg aaactccgcc gactaatcgc tatcctgttc agacctatgt
    12541 tttggaaaag aatgatagtg tcattcgtga tgctgtcttg cgtgaaatgg agcgtggagg
    12601 tcaagtttac tatctttaca acaaagttga cacaattgtt cagaaggttt cagaattaca
    12661 ggagttgatt ccggaggctt cgattggata tgttcatggt cgaatgagtg aagtccagtt
    12721 ggaaaatact ctattagact ttattgaggg acaatacgat atcttggtga cgactactat
    12781 tattgagaca ggggtggaca ttccaaatgc taatacttta tttattgaaa atgcggacca
    12841 tatgggcttg tcaaccttat atcagttaag aggaagagtc ggtcgtagta atcgtattgc
    12901 ttatgcttat ctcatgtatc gtccagaaaa atcaatcagt gaagtctctg aaaagagatt
    12961 agaagcgatt aaaggattta cagaattggg ctctggcttt aagattgcaa tgcgagatct
    13021 ttcgattcgt ggagcaggaa atcttttagg aaaatcccag tctggtttca ttgattctgt
    13081 tggttttgaa ttgtattcgc agttattaga ggaagctatt gctaaacgaa acggtaatgc
    13141 taacgctaac acaagaacca aagggaatgc tgagttgatt ttgcaaattg atgcctatct
    13201 tcctgatact tatatttctg atcaacgaca taagattgaa atttacaaga aaattcgtca
    13261 aattgacaac cgtgtcaatt atgaagagtt acaagaggag ttgatagacc gttttggaga
    13321 atacccagat gtagtagcct atcttttaga gattggtttg gtcaaatcat acttggacaa
    13381 ggtctttgtt caacgtgtgg aaagaaaaga taataaaatt acaattcaat ttgaaaaagt
    13441 cactcaacga ctgtttttag ctcaagatta ttttaaagct ttatccgtaa cgaacttaaa
    13501 agcaggcatc gctgagaata aggaattaat ggagcttgta tttgatgttc aaaataagaa
    13561 agattatgaa attttagaag gtctgctgat ttttggagaa agtttattag agataaaaga
    13621 gtctaaggaa gaaaattcca tttgatattt ttcttctata aaatagataa aaatggtaca
    13681 ataataaatt gaggtaataa ggatgagatt agataaatat ttaaaagtat cgcgaattat
    13741 caagcgtcgt acagtcgcaa aggaagtagc agataaaggt agaatcaagg ttaatggaat
    13801 cttggccaaa agttcaacgg acttgaaagt taatgaccaa gttgaaattc gctttggcaa
    13861 taagttgctg cttgtaaaag tactagagat gaaagatagt acaaaaaaag aagatgcagc
    13921 aggaatgtat gaaattatca gtgaaacacg ggtagaagaa aatgtctaaa aatattgtac
    13981 aattgaataa ttcttttatt caaaatgaat accaacgtcg tcgctacctg atgaaagaac
    14041 gacaaaaacg gaatcgtttt atgggagtgg tattgatttt gattatgcta ttatttatct
    14101 tgccaacttt taatttagcg cagagttatc agcaattact ccaaagacgt cagcaattag
    14161 cagacttgca aactcagtat caaactttga gtgatgaaaa ggataaggag acagcatttg
    14221 ctaccaagtt gaaagatgaa gattatgctg ctaaatatac acgagcgaag tactattatt
    14281 ctaagtcgag ggaaaaagtt tatacgattc ctgacttgct tcaaaggtga taaaatggaa
    14341 aatttattag acgtaataga gcaatttttg agtttgtcag atgaaaagct ggaagaattg
    14401 gctgataaaa atcaattatt gcgtttacaa gaagaaaagg aaaggaagaa tgcgtaaatt
    14461 cttaattatt ttgttgctac caagtttttt gaccatttca aaagtcgtta gcacagaaaa
    14521 agaagtcgtc tatacttcga aagaaattta ttacctttca caatctgact ttggtattta
    14581 ttttagagaa aaattaagtt ctcccatggt ttatggagag gttcctgttt atgcgaatga
    14641 agatttagta gtggaatctg ggaaattgac tcccaaaata agttttcaaa taaccgagtg
    14701 gcgcttaaat aaacaaggaa ttccagtatt taagctatca aatcatcaat ttatagctgc
    14761 ggacaaacga tttttatatg atcaatcaga ggtaactcca acaataaaaa aagtatggtt
    14821 agaatctgac tttaaactgt acaatagtcc ttatgattta aaagaagtga aatcatcctt
    14881 atcagcttat tcgcaagtat caatcgacaa gaccatgttt gtagaaggaa gagaatttct
    14941 acatattgat caggctggat gggtagctaa agaatcaact tctgaagaag ataatcggat
    15001 gagtaaagtt caagaaatgt tatctgaaaa atatcagaaa gattctttct ctatttatgt
    15061 taagcaactg actactggaa aagaagctgg tatcaatcaa gatgaaaaga tgtatgcagc
    15121 cagcgttttg aaactctcct atctctatta tacgcaagaa aaaataaatg agggtcttta
    15181 tcagttagat acgactgtaa aatacgtatc tgcagtcaat gattttccag gttcttataa
    15241 accagaggga agtggtagtc ttcctaaaaa agaagataat aaagaatatt ctttaaagga
    15301 tttaattacg aaagtatcaa aagaatctga taatgtagct cataatctat tgggatatta
    15361 catttcaaac caatctgatg ccacattcaa atccaaaatg tctgccatta tgggagatga
    15421 ttgggatcca aaagaaaaat tgatttcttc taagatggcc gggaagttta tggaagctat
    15481 ttataatcaa aatggatttg tgctagagtc tttgactaaa acagattttg atagtcagcg
    15541 aattgccaaa ggtgtttctg ttaaagtagc tcataaaatt ggagatgcgg atgaatttaa
    15601 gcatgatacg ggtgttgtct atgcagattc tccatttatt ctttctattt tcactaagaa
    15661 ttctgattat gatacgattt ctaagatagc caaggatgtt tatgaggttc taaaatgagg
    15721 gaacaagatt ttttaaatca ttttctcaag aagggatatt tcaaaaagca tgctaaggcg
    15781 gttctagctc tttctggtgg attagattct atgtttctat ttaaggtatt gtctacttat
    15841 caaaaagagt tagagattga attgattctg gctcatgtga atcataagca gagaattgaa
    15901 tcagattggg aagaaaagga attaaggaag ttggctgctg aagcagaact tcctatttat
    15961 atcagcaatt tttcaggaga attttcagaa gcgcgtgcac gaaattttcg ttatgatttt
    16021 tttcaagagg tcatgaaaaa gacaggtgcg acagctttag tcactgccca ccatgctgat
    16081 gatcaggtgg aaacgatttt gatgcgcttg attcgaggaa ctcgcttgcg ctatctatca
    16141 ggaattaagg agaagcaagt agtcggagag atagaaatca ttcgtccctt cttgcatttt
    16201 cagaaaaaag actttccatc aatttttcac tttgaagata catcaaatca ggagaatcat
    16261 tattttcgaa atcgtattcg aaactcttac ttaccagaat tggaaaaaga aaatcctcga
    16321 tttagggatg caatcttagg catcggcaat gaaattttag attatgattt ggcaatagct
    16381 gaattatcta acaatattga tgtggaaaat ttacagcagt tattttctta ctctgagtct
    16441 acacaaagag ttttacttca aacttatctg aatcgttttc cagatttgaa tcttacaaaa
    16501 gctcagtttg ctgaagttca gcagatttta aaatctaaaa gccagtatcg tcatccgatt
    16561 aaaaatggct atgaattgat aaaagagtac caacagtttc agatttgtaa aatcagtccg
    16621 caggctgatg aaaaggaaga tgaacttgtg ttacactatc aaaatcaggt agcttatcaa
    16681 ggatatttat tttcttttgg acttccatta gaaggtgaat taattcaaca aatacctgtt
    16741 tcacgtgaaa catccataca cattcgtcat cgaaaaacag gagatgtttt gattcaaaat
    16801 gggcatagaa aaaaactcag acgtttattt attgatttga aaatccctat ggaaaagaga
    16861 aactctgctc ttattattga gcaatttggt gaaattgtct caattttggg aattgcgacc
    16921 aataatttga gtaaaaaaac gaaaaatgat ataatgaaca ctgtacttta tatagaaaaa
    16981 atagataggt aaaaaatgtt agaaaacgat attaaaaaag tcctcgtttc acacgatgaa
    17041 attacagaag cagctaaaaa actaggtgct caattaacta aagactatgc aggaaaaaat
    17101 ccaatcttag ttgggatttt aaaaggatct attcctttta tggctgaatt ggtcaaacat
    17161 attgatacac atattgaaat ggacttcatg atggtttcta gctaccatgg tggaacagca
    17221 agtagtggtg ttatcaatat taaacaagat gtgactcaag atatcaaagg aagacatgtt
    17281 ctatttgtag aagatatcat tgatacaggt caaactttga agaatttgcg agatatgttt
    17341 aaagaaagag aagcagcttc tgttaaaatt gcaaccttgt tggataaacc agaaggacgt
    17401 gttgtagaaa ttgaggcaga ctatacctgc tttactatcc caaatgagtt tgtagtaggt
    17461 tatggtttag actacaaaga aaattatcgt aatcttcctt atattggagt attgaaagag
    17521 gaagtgtatt caaattagaa agaataatct ttaatgaaaa aacaaaataa tggtttaatt
    17581 aaaaatcctt ttctatggtt attatttatc tttttccttg tgacaggatt ccagtatttc
    17641 tattctggga ataactcagg aggaagtcag caaatcaact atactgagtt ggtacaagaa
    17701 attaccgatg gtaatgtaaa agaattaact taccaaccaa atggtagtgt tatcgaagtt
    17761 tctggtgtct ataaaaatcc taaaacaagt aaagaaggaa caggtattca gtttttcacg
    17821 ccatctgtta ctaaggtaga gaaatttacc agcactattc ttcctgcaga tactaccgta
    17881 tcagaattgc aaaaacttgc tactgaccat aaagcagaag taactgttaa gcatgaaagt
    17941 tcaagtggta tatggattaa tctactcgta tccattgtgc catttggaat tctattcttc
    18001 ttcctattct ctatgatggg aaatatggga ggaggcaatg gccgtaatcc aatgagtttt
    18061 ggacgtagta aggctaaagc agcaaataaa gaagatatta aagtaagatt ttcagatgtt
    18121 gctggagctg aggaagaaaa acaagaacta gttgaagttg ttgagttctt aaaagatcca
    18181 aaacgattca caaaacttgg agcccgtatt ccagcaggtg ttcttttgga gggacctccg
    18241 gggacaggta agactttgct tgctaaggca gtcgctggag aagcaggtgt tccattcttt
    18301 agtatctcag gttctgactt tgtagaaatg tttgtcggag ttggagctag tcgtgttcgc
    18361 tctctttttg aggatgccaa aaaagcagca ccagctatca tctttatcga tgaaattgat
    18421 gctgttggac gtcaacgtgg agttggtctc ggcggaggta atgacgaacg tgaacaaacc
    18481 ttgaaccaac ttttgattga gatggatggt tttgagggaa atgaagggat tatcgtcatc
    18541 gctgcgacaa accgttcaga tgtacttgat cctgcccttt tgcgtccagg acgttttgat
    18601 agaaaagtat tggttggccg tcctgatgtt aaaggtcgtg aagcaatctt gaaagttcac
    18661 gctaagaaca agcctttagc agaagatgtt gatttgaaat tagtggctca acaaactcca
    18721 ggctttgttg gtgctgattt agagaatgtc ttgaatgaag cagctttagt tgctgctcgt
    18781 cgcaataaat cgataattga tgcttcagat attgatgaag cagaagatag agttattgct
    18841 ggaccttcta agaaagataa gacagtttca caaaaagaac gagaattggt tgcttaccat
    18901 gaggcaggac ataccattgt tggtctagtc ttgtcgaatg ctcgcgttgt ccataaggtt
    18961 acaattgtac cacgcggccg tgcaggcgga tacatgattg cacttcctaa agaggatcaa
    19021 atgcttctat ctaaagaaga tatgaaagag caattggctg gcttaatggg tggacgtgta
    19081 gctgaagaaa ttatctttaa tgtccaaact acaggagctt caaacgactt tgaacaagcg
    19141 acacaaatgg cacgtgcaat ggttacagag tacggtatga gtgaaaaact tggcccagta
    19201 caatatgaag gaaaccatgc tatgtttggt gcacagaatc ctcaaaaatc aatttcagaa
    19261 caaacagctt atgaaattga tgaagaggtt cgttcattat taaatgaggc acgaaataaa
    19321 gctgctgaaa ttattcagtc aaatcgtgaa actcacaagt taattgcaga agcattattg
    19381 aaatacgaaa cattggatag tacacaaatt aaagctcttt acgaaacagg aaagatgcct
    19441 gaagcagtag aagaggaatc tcatgcacta tcctatgatg aagtaaagtc aaaaatgaat
    19501 gacgaaaaat aaccctgaga gaggctggag cctctctttt ttgtgcagtt taggagctaa
    19561 agggaacaga atggagaaaa tggaacaaat gtgttttcta atctgttaga ctgtatctag
    19621 aaaggggaaa attatgatta aagaattgta tgaagaagtc caagggactg tgtataagtg
    19681 tagaaatgaa tattaccttc atttatggga attatcggat tgggaccaag aaggcatgct
    19741 ctgcttacat gaattgatta gtagagaaga aggactggta gacgatattc cacgtttaag
    19801 gaaatatttc aaaaccaagt ttcgaaatcg aattttagac tatatccgta agcaggaaag
    19861 tcagaagcgt aaatacgata aagaacccta tgaagaagtg ggtgagctca gtcatcgtat
    19921 aagtgagggg gg
//
LOCUS       DAVKQB010000042        19451 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKQB010000042 DAVKQB010000000
VERSION     DAVKQB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19451
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.042"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            108..583
                     /locus_tag="VW528_001549"
                     /pseudo
     CDS             108..583
                     /locus_tag="VW528_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            621..923
                     /locus_tag="VW528_001550"
     CDS             621..923
                     /locus_tag="VW528_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9912356.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            931..1732
                     /locus_tag="VW528_001551"
                     /pseudo
     CDS             931..1732
                     /locus_tag="VW528_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1781..2245
                     /locus_tag="VW528_001552"
     CDS             1781..2245
                     /locus_tag="VW528_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9912357.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2402..2827
                     /gene="lacA"
                     /locus_tag="VW528_001553"
     CDS             2402..2827
                     /gene="lacA"
                     /locus_tag="VW528_001553"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9912358.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2858..3373
                     /gene="lacB"
                     /locus_tag="VW528_001554"
     CDS             2858..3373
                     /gene="lacB"
                     /locus_tag="VW528_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9912359.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3384..4313
                     /locus_tag="VW528_001555"
     CDS             3384..4313
                     /locus_tag="VW528_001555"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9912360.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            4315..5295
                     /gene="lacD"
                     /locus_tag="VW528_001556"
     CDS             4315..5295
                     /gene="lacD"
                     /locus_tag="VW528_001556"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9912361.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5529..6365
                     /locus_tag="VW528_001557"
     CDS             5529..6365
                     /locus_tag="VW528_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9912362.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6401..6718
                     /locus_tag="VW528_001558"
     CDS             6401..6718
                     /locus_tag="VW528_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9912363.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6720..8414
                     /locus_tag="VW528_001559"
     CDS             6720..8414
                     /locus_tag="VW528_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9912364.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            8623..10029
                     /gene="lacG"
                     /locus_tag="VW528_001560"
     CDS             8623..10029
                     /gene="lacG"
                     /locus_tag="VW528_001560"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9912365.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTDYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            10119..10331
                     /locus_tag="VW528_001561"
     CDS             10119..10331
                     /locus_tag="VW528_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9912366.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
     gene            complement(10610..11371)
                     /locus_tag="VW528_001562"
     CDS             complement(10610..11371)
                     /locus_tag="VW528_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9912367.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(11659..12621)
                     /gene="nrdF"
                     /locus_tag="VW528_001563"
     CDS             complement(11659..12621)
                     /gene="nrdF"
                     /locus_tag="VW528_001563"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9912368.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(12809..14968)
                     /gene="nrdE"
                     /locus_tag="VW528_001564"
     CDS             complement(12809..14968)
                     /gene="nrdE"
                     /locus_tag="VW528_001564"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9912369.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(15049..15267)
                     /gene="nrdH"
                     /locus_tag="VW528_001565"
     CDS             complement(15049..15267)
                     /gene="nrdH"
                     /locus_tag="VW528_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9912370.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPEKLKQLA"
     gene            15618..15881
                     /locus_tag="VW528_001566"
     CDS             15618..15881
                     /locus_tag="VW528_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9912371.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            15887..17620
                     /gene="ptsP"
                     /locus_tag="VW528_001567"
     CDS             15887..17620
                     /gene="ptsP"
                     /locus_tag="VW528_001567"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9912372.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            17875..>19451
                     /locus_tag="VW528_001568"
     CDS             17875..>19451
                     /locus_tag="VW528_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9912373.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPS
                     NPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLG
                     AKKTDLPSSDREFYNKTYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKL
                     VDDILAFLAPIRHPERLG"
BASE COUNT         6196 a         3453 c         4007 g         5795 t
ORIGIN      
        1 aacaaaaata taaaaaaaga gacaaaaaag aacagaaagt aattaacaac gatttcatat
       61 agtgttatac ttatagcata aagttaatgg aaagaaggga aaaccttatg gtattagatt
      121 atttctttga caaaaacctt gtgttttgct tagaagcgga taatcaagaa taactctttg
      181 atcaggtagc aagcttgttg gaggaacgag aaattgtaac tccaacttat cgtgaagctt
      241 tgatcacgcg tgaaaagtca tttccaactg gtttagatat ggaatttcta ggaaaggact
      301 tgcaaatgta atgattcctc acacagatat tgttcataat ctagctgaga aagtggtggt
      361 tgttcgatta gagaaaccgg tgactttcca taatatgatt gcgccaggta aggaagtaga
      421 agtatccttg ctcttcttta tcatcaataa ttcaagttca agtcaaacga acattcttgc
      481 tcagttgatg gactttttca caggaaatgg acatcttgaa gacctatcaa aaatttccga
      541 accagaaaaa ctttatgctt atatagatga agcaatcgct taatcttgtc tattaaaaaa
      601 taaaatcgga ggaagtctat atgattaaaa ttcttgctgc ctgcggtgca ggtgttaact
      661 caagtcacca aattaaaagt gctctagaag aagaactttc aaaccgtggt tacgatgttc
      721 actgtgatgc agttatggtg aaagatgtaa acgaagacct tatgaaaggt tacgatatct
      781 ttacaccaat cgctgcaaca gaccttggtt ttgaaccagg tatcccagtt atcgaagctg
      841 ggccaatctt attccgtatc ccagcaatga gcgttccagt atttgacaat attagacttc
      901 ctgcgaaaca aaatatggta tagtagttct atgaatgatg aagcaagtaa acaactaact
      961 gatgcacgat ttaagcgtct tgttggtgtt cagcgtacca cttttgaaga gatgttagct
     1021 gtattaaaaa cagcttatca acttaaacac gcaaaaggtg gacgaaaacc taaattaagc
     1081 ctagaagacc ttcttatggc cactcttcaa tagtgcgaga atatcgaact tatgaacaaa
     1141 ttgcggctga ttttggtatt cacgaaagca actttatccg tcggagccaa tgggttgaag
     1201 taactcttgt tcaaagtggt gttacggttt caagaactcc tctcagctct gaggacacgg
     1261 taatgattga tgcgacggaa gtacaaatca atcgccctaa aaaagaatta gcgaatgatt
     1321 ctggtaaaaa gaaatgccac gctatgaagg ctcaagcgat tgtcacaagt caagggagat
     1381 tgtttctttg gatatcactg tgaactattg tcatgatatg aagttgttca aaatgagtcg
     1441 cagaaatatc ggacaagctg gtaaaatctt ggctgacagt ggttatcaag ggctcatgaa
     1501 gatatatcct caagtacaaa ctccacgtaa atccagcaaa ctcaagccgc taacagttga
     1561 agataaaacc tataaccatg cgctatctaa ggagagaagc aaggttgaga acatctttgc
     1621 caaagtaaaa acgtttaaaa tgatttcaac aacctatcga aatcatcgta aacgcttcgg
     1681 attacgaatg aatttgattg ctggcattat caatcatgaa ctaggattct agttttgcag
     1741 gaagtctaat aaagaaacta gcaaggtcag gccttacttt attggtggac atccaggatt
     1801 taattgtcct ctacttgatg atgaagtcta tgaagattat tatctagagt ttgagaaaga
     1861 agagacttgc tctgttccac gtcctttccc agaaacaggt atgttggatt tccaagatag
     1921 aagtccatgg ctagagggac aaaaagaaat agatcttagt tatgatcttt tcagcaaaga
     1981 tgcagtgact ttggatgaat tgcaatctcg aacaattgcc cttcgttctc ttaaacatga
     2041 taggggattg aaagtgcatt ttgcagaatt tccaaacctc atcatctggt caactttgaa
     2101 caagggacct ttcattacct ttgaaccatg gtctggcttg tcaacattcc ttgaagaagg
     2161 agatcattta gaagataaga agaatgtttg tctcttagaa gccaatcagg ttgaagaatt
     2221 agggtttgag atagaagttt tataaaaaag taaaaaataa ataacaaaaa caaacacaaa
     2281 ctgttgactt cttcaaacaa tagtagtata ttatgtttgt gaggactaat tgactgtttt
     2341 ttaaagacag ccaatacact gctggaatcc agcatagaaa aaataaaaag gagtatacaa
     2401 tatgtctatt gttatcggtg cagatgctgc aggtttgaga ttgaaagaag ttgtgaaaga
     2461 cttcttagaa aaagaaaact tccaccttgt ggatgttaca gctgaaggtc aagactttgt
     2521 tgatgtgact ctcgctgttg ctgcagaagt aaacaaagaa gaacaaaacc ttggtatcgt
     2581 gattgatgct tatggagctg gtccatttat ggttgcaact aagatcaaag gaatggttgc
     2641 tgcagaagtt tctgatgaac gttcagctta tatgactcgt ggccacaaca actcacgtat
     2701 gatcactatg ggtgcacaac ttgttggtga tgaattggct aaaaacatcg ctaaaggatt
     2761 tgttaatggt aaatacgacg gtggtcgtca ccaaatccgg gttgacatgt tgaacaaaat
     2821 gggctaatta gattatagta agaaaggtaa gttaaaaatg agaattgcaa ttggatgtga
     2881 ccacatcgta acagatgaaa aaatggcggt ttcagaattt ttgaaatcaa aaggatatga
     2941 agtcattgac tttggtacat atgaccatac acgtactcac tacccaatct ttggtaaaaa
     3001 agtaggggaa gctgtaacta gcggtcaagc tgatcttgga gtatgtatct gtggtactgg
     3061 tgttggtatc aacaacgctg taaataaagt tccaggtgtt cgttctgcct tggttcgtga
     3121 tatgacaaca gccctttatg ctaaagaaca attgaacgct aacgttattg gttttggtgg
     3181 taaaattact ggtgaattgc ttatgtgtga tatcatcgaa gctttcatcc atgctgaata
     3241 caaaccaact gaagaaaaca aaaaattgat tgcgaaaatt gaacatgttg aaagtcacaa
     3301 tgctcaacaa acagacgcaa acttcttcac tgaattcctt gaaaaatggg atcgcggaga
     3361 ataccacgac taagaggtaa cctatgattt taacagttac aatgaaccca tccattgata
     3421 tttcctatcc cttggatgag ttgaagattg atactgtcaa tcgtgtggtg gatgtaacca
     3481 aaacggctgg tggtaaggga ctcaatgtta cccgagtact ttcagaattt ggcgattctg
     3541 ttcttgctac tggtttagtg ggtggcaaac ttggtgagtt tttggttgaa catatcgata
     3601 atcaagtaaa gaaagatttc ttctcaattc agggagaaac tcgtaactgt atcgctattc
     3661 tccacggaga caaccaaaca gaagttcttg aaaaaggtcc tgaagtattg gaacaggaag
     3721 gtcaagactt tttggaacat ttcaaaaaac tcttggagtc agttgaagta gtggccatct
     3781 caggtagtct gccagctggc cttccagttg attactatgc gagcttggta gaacttgcta
     3841 atcaagctgg caagcctgta gtcttggact gctcaggtgc agcacttcag gctgttcttg
     3901 aatcacccca taaaccaaca gtcatcaaac caaataatga agaattgtct cagcttcttg
     3961 gaagagaagt ttctgaggat ttggatgaat taaaagaagt acttcaagaa cctttgtttg
     4021 cagggattga atggattatc gtttcacttg gtgccaacgg tacttttgcc aaacatggtg
     4081 acactttcta caaggtagat attcctagaa ttcaggtggt aaatcctgtt ggatctggag
     4141 actctactgt ggcaggaatt tcttcaggac ttcttcacaa agaatcggat gcagaattac
     4201 tcatcaaggc aaatgtcctt ggtatgctca atgctcaaga aaaaatgact ggtcatgtca
     4261 acatggccaa ctatcaagtt ctatatgatc aattaatagt aaaagaggta taaaatggct
     4321 ttaacagaac aaaaacgtgt acgcttagaa aaactttctg atgaaaatgg tatcatctca
     4381 gctcttgcat ttgaccaacg tggtgctttg aaacgcctca tggctcaaca ccaaacagaa
     4441 gaaccaactg tggcccaaat ggaagaactt aaagtcttgg tagcagatga attgactaaa
     4501 tacgcttcat ctatgcttct tgaccctgag tatggacttc cagcaactaa agctcttgat
     4561 gaaaaagctg gtcttctcct tgcttatgaa aaaacaggtt atgacacaac aagcacaaaa
     4621 cgcttgccag actgcttgga tgtctggtct gcaaaacgta ttaaagaaga aggtgcagat
     4681 gcagttaaat tcttgcttta ctatgatgta gatagctcag acgaactcaa tcaagaaaaa
     4741 caagcctaca tcgaacgcat cggttctgag tgtgtggctg aagatatccc attcttcctt
     4801 gaaattcttg cttacgatga aaaaattgcg gatgcaggtt ctgtagaata cgcgaaagta
     4861 aaaccacaca aagttatcgg cgctatgaaa gtcttttcag acccacgctt taacattgat
     4921 gtcttgaaag ttgaagttcc tgttaacatt aaatatgttg aaggcttcgc tgaaggtgaa
     4981 gtagtttata cacgtgaaga agcagcagcc ttcttcaaag cgcaagatga agcaacgaac
     5041 ttgccataca tctacttgag tgctggtgta tcagctaaac tcttccaaga tactcttgta
     5101 tttgctcatg aatcaggtgc aaactttaac ggagttcttt gtggccgtgc aacatgggct
     5161 ggatcagttg aagcatacat caaagatggt gaaacagcag ctcgcgaatg gcttcgcaca
     5221 actggatttg aaaacattga tgagctcaac aaagttcttc aaacaacagc aacttcatgg
     5281 aaagaacgtg tgtaagaaag tcctcctagt ttaggaacat gaatctaaaa aaatttaaaa
     5341 aaagttgtat gtaaaggttt acaaaataac ttacttgtgc tatacttaaa tcacaagtta
     5401 atacaaggtg agtgttacta agtaatatta ggcatgatca caggtgaatt agaaatcagc
     5461 tgattttcta gttcatttgt ggtcattttt tgtactcata tacctttaag atataaaagg
     5521 aggttgacat gtatcgaatt ctaaatccaa tgaatcacaa tgtctcgctt gtcagaaatg
     5581 ataagggaga agaggtgatt gtaattggta aggggattgc attcggaaag aagaaggggg
     5641 atttgattgc tgaaaatcat gttgagaaaa tctttcggat gaagaccgaa gagtccagag
     5701 aaaactttat ggctcttctc aaagatgttc cgcttgattt tatcacagtg acctatgaaa
     5761 tcattgataa gctatcaaag aaatatcatt atccgattca agagtatctc tatgtaacct
     5821 tgacagatca tatttactgt tcttatcaag ctctaactca aggaaggtac aaggatagta
     5881 atctgccaga tatttctgct aagtatcctg tcgcttttca aatcgcaaat gaagcttttg
     5941 aaatttactg tcagaagcta gcagatcatt ttcctgagga cgaaattatt cggattgctt
     6001 atcatttcat taatgctgaa ggtgaaaatg aagtggaact tgtggagtcg attgataaga
     6061 ggaaagaaat tctcaggaat gttgaagaag ttttaacgga ctatgcaatt caacgaacta
     6121 aaaagaataa ccatttctat gatcgcttta tgatccattt gaattatttc ttggattatt
     6181 tagacagatc tagagatgat aaccaatcac ttctggatat ggaagatcat attaaacaat
     6241 cctatccaaa agccttcgag attggttcca agatctatga tgtgattacg caacatacgg
     6301 gtcttgattt gtataaaagt gaacgagttt atctagttct acatatccaa cgtttattgt
     6361 cataaaaatt tatttaaaac tatataagga gaattctatc atgaatagag aagaagtaac
     6421 attgttaggt tttgaaatcg tagcctatgc tggcgatgct cgttcaaaac tattggaagc
     6481 cttgaaggct gctgaagctg gtgattttga aaaagcggac gctctggtag aggaagctgg
     6541 tagctgtatt gcagaggctc accacgcgca aacaagtcta ttgactaagg aagcttctgg
     6601 tgaggactta gcgtatagtg tgaccatgat gcatggtcaa gatcacttga tgacaactat
     6661 cttgttaaaa gacctaatgc accatttaat tgaactttac aagagaggag ttaaataaaa
     6721 tggataagtt aataattttt attgagaaag gaaagccttt ctttgagaaa ttatcgagaa
     6781 atatatactt aagagcgata aaagatggtt ttatttccag tatgccagca gtattattct
     6841 caagtatttt tatactaata gctgctgtgc ccaatatttt tgggtttaaa tggtctgatg
     6901 aacagttagc ttttattttg aaaccttaca actactcaat ggggatttta gctctcttag
     6961 tagctgggac aactgcaaaa tcgttaacag attcagttaa tacacgtagt atggagaaaa
     7021 ccaaccaaat caactacatg tcaacttttt tagcagctgt tgttggattg ttaatactgg
     7081 cagctgatcc aattgaaggt ggatttgcta atggtctttt aggtactaga ggattactta
     7141 ctgcattttt ggcagctttt attacagtaa atatctataa agtatgtatt aaaaataatg
     7201 taaccattag acttcctgaa gaggttccac caaatattgc acaagttttt aaagatgtta
     7261 ttccgtttgc cttatctgtt ctttcaatat atggattgga tttaattgtt agaaatattt
     7321 ttggtacaaa tgtagcagaa tctgttggta aaatattagc ccctctattt tcagctactg
     7381 atggttatat cggtttagct attgtatttg gtgcttatgc tttcttttgg tttgttggaa
     7441 ttcatgggcc atctgttgta gaacctttaa ttgtagctat cagttatgca aatatagaag
     7501 ctaatgttca gcttgtgcaa gctggtatgc acgcagataa aattttaaat ccagttactc
     7561 aaacttttgt tgtaacaatg ggtggtactg gtgcaacact agtagttcca tttatgttta
     7621 tgtggttatg taaatcaaaa agaaatagaa ttgtcggacg tgcgtctgta gttcctactt
     7681 tctttggcgt aaatgaacca atcctatttg gtgctccaat tgttttaaat cctatcttct
     7741 ttattccgtt tgttactgct cctattatca atgtttggat tatgaaattt tttgtagatg
     7801 ttctacaaat gaatagtttc agtattattt taccttggac aacacctgct ccaattggta
     7861 ttgttatggg aacggcgctg gctccattgt cattcgtctt ggctataact ttaatcatta
     7921 tcgacacttt aatctattat ccatttgtta aagtttatga ccatcaaatt ttagaagaag
     7981 aacggaaagg aaactcttca tctgaattga aagaaaaagt tgctgcaaac ttcaacactg
     8041 caaaagcgga tgctattctt gaaaaagcgg gtgtagaagc agcacaaaat acaatcacta
     8101 aagaaacaaa tgtcctcgtt ctctgtgcag gtggaggaac aagtggtctc cttgcaaatg
     8161 ctttgaataa ggcagcagca gaatacaatg tccctgtgaa agcagcagca ggcggctatg
     8221 gtgctcaccg tgaaatgtta ccagagtttg atcttgttat ccttgcccct caagttgctt
     8281 caaactttga agatatgaaa gcagaaacag ataagctcgg tattaaacta gcgaaaacag
     8341 aaggcgctca atacatcaaa ttaactcgtg atggaaaagg tgctcttgca ttcgtacaag
     8401 cgcaattcga ttaaggctag agactctgaa atagtctcct atcgttacgg aaatcgctat
     8461 ggcgaatttc ctattattaa ttcgtcggta aaaagatatc gtttttacct cctcatgtca
     8521 caattcggtg acttggtaca agaagtgaga tggagaagga tggctcattg actcctctcc
     8581 tctcactttt actttattta aatcaagaaa taggtgaaaa aaatgacaaa aacacttcca
     8641 aaagacttta tttttggtgg cgcaacagct gcctatcaag cagaaggtgc tacacatact
     8701 gatggtaaag gaccagtagc gtgggataaa tatcttgaag ataactactg gtacactgca
     8761 gaaccagcca gtgattttta caatcgatat ccagttgacc tcaagctagc agaagagtat
     8821 ggtgtcaatg gtattcgaat ttctattgct tggtcacgta ttttcccgac tgattacggc
     8881 caagtaaatg ctaaaggtgt tgagttttat cataatttat ttgcagagtg tcacaaacgt
     8941 catgttgagc cttttgtaac tcttcatcac tttgacacgc cagaagctct ccactcaaat
     9001 ggagacttct taaaccgtga aaatatcgaa cattttgtag actacgctgc cttctgtttt
     9061 gaagaatttc cagaagtaaa ctattggaca acctttaatg aaattggacc aatcggtgat
     9121 ggtcaatatt tggttgggaa attccctcca ggtatccagt acgaccttgc caaagtcttt
     9181 caatcacacc acaatatgat ggtgtctcat gcacgcgcgg taaaattgta caaagagaaa
     9241 ggctataaag gggaaattgg tgttgttcac gccctgccaa ctaaatatcc tctagatcct
     9301 gaaaatccag cagatgttcg tgcagctgag ttggaagata tcatccacaa taaattcatc
     9361 ttagacgcaa cttatctagg tcgctattca gctgaaacca tggaaggtgt caaccatatc
     9421 ttatcagtca atggtggtag cttggatctt cgtgaagaag attttacagc attagaagct
     9481 gcaaaagact tgaatgattt cctaggaatc aactactata tgagtgactg gatggaagcc
     9541 tttgatggag aaactgaaat tatccataat ggtaaaggtg aaaaaggaag ctctaagtat
     9601 caaatcaaag gtgttggtcg tcgtgtagct cctgactatg taccacgcac ggattgggat
     9661 tggattatct accctcaagg tttgtatgac caaatcatgc gtgtgaagaa agattatcct
     9721 aactacaaga agatttacat cactgaaaat ggtctcggct ataaagatga gttcgttgat
     9781 aacactgttt acgatgatgg tcgtattgat tacgtgaaac aacacttgga gatcttgtct
     9841 gatgcgattg cagatggagc taatgtaaaa ggttacttca tttggtcatt aatggatgtc
     9901 ttctcatggt caaacggtta tgagaaacgt tatggtctct tctacgtaga ttttgaaact
     9961 caagaacgtt atcctaagaa atcagctcac tggtacaaga aagtagcgga aactcagatt
    10021 atagactagt agaattagtc attagatata gaattttagt gagtcaaaaa gatgttcaaa
    10081 gattttatcc aatctattta tgaaaaaaag tttatattat aaatttcgaa aaatgctctc
    10141 aaataccgtg tttgacgagt gaagaattga aaagtcttgg aaaatggtat gtctcgactg
    10201 gtaaagaatg gatttgtcat tcagatgatg agctggaaga atttaaaaat ctatttttaa
    10261 attttatcaa tcctgaagaa tgggatacta tctcctttga ttcagatttt atgccgtttc
    10321 aacaatcgta accaatttct caaaaaagtt aaatcttata tttagtactc tgtaaaactc
    10381 ttatctaatc acgttgctta tactcaatga aaatcaaaga gcaactttaa actaggaagc
    10441 gagtcgcaga tttctcaatg catagctttg aggaattggg caaaaagtct ttgatataga
    10501 aaaaagcata gtatcaggtg ttgaaacacc tgatactatg ctttttattg tgggaagatt
    10561 tacttttttt cttctgaaat tgagttgtta cccaggctct ttcagtttat taaggcttga
    10621 tgactttaat gtgtttagat agcttaaaaa ggattgaatc acttagttta gaatctgaaa
    10681 caatagtatc aagatttgat atattataaa aagtataaaa atcaaactta ttgaacttac
    10741 tatgatctgc gagtaaatat tttttattag aattatttaa agcgatgcgt tgagcctctc
    10801 cctcttcctc gctaaaagta gctagagctc cgttttgaat accattacag ctaacgaaag
    10861 ctttagaaaa ttggagatta gagagatttt gtagggtcaa tgtgccaaca aaagcacctg
    10921 taatatcgcg ataatttcca cctatcaaaa tcaaatctgt taattttcgt tcgcttaaaa
    10981 tcagaaaaac aggtagactg ttggttacga cgcggatatt gtcaataggc aactcacgcg
    11041 caaaaaactc taatgttgtt cctggtccaa tgaaaatagt ttctctttct tctactagac
    11101 tgcctgcaaa atgggctatt tcttgttttt ctgccgtttg gagggcttgt ttttcaatat
    11161 ttgatcgctc attagtcaaa agggagttgg ttcgaagttt ttcagctcca ccatgcacac
    11221 gaatcagcaa atctttatca gctaattcct gtaaatagcg ccttgcagtc atatctgaaa
    11281 cggctatttc gtccataatc tgtttaactg ttatggttcc tttactattt actatctcta
    11341 aaattttggt taatttttct tgtttgagca tattatcacc tcgtttccta ctactatctt
    11401 accataaaca aactcatcat tcaaatacaa aaacaacaaa atgaaacaaa aacaaaaata
    11461 tcgaagtttg ttttcaaaac tttcgatatt tttgttgggt tattattttg ttgtttctag
    11521 tttacttttt gatggttaag agtggtggag aattatactc aatgaaaatc aaagagcaaa
    11581 ctaggaagct agccgcaggc tgtacttgag tacggcaagg cgaagctgac gtggtttgaa
    11641 tttgattttc gaagagtatt agtccaaacc gtagttgtag tcatcatctt gcatggcttc
    11701 aacttcgcca agaaggtaac catttccgac ttgagagaag aagtcatggt tggaagttcc
    11761 tgttgaaata ccgttcataa cgattgggtt gacatcttca gctgaatctg ggaaaagtgg
    11821 atcttgtccc atgttcatga gagctttatt ggcattgtag cgaaggaagg ttttaacctc
    11881 ttcagtccaa ccaacaccgt cataaagact ctctgtgtag ccttcttcat tttcataaag
    11941 agtatagagt aggtcgtaca tccattcttt gagtttttct tgctcttctt caggtaattc
    12001 attgaaacca agttggaatt tgtaaccaat gtaggttccg tgaacagact cgtcacgaat
    12061 aatcaattta atgatttctg caacgttggc aagtttgttg ttaccgagat agtagagggg
    12121 agtgaagaaa ccagagtaga agaggaaggt ttcgaggaag acgctggcaa ctttcttttc
    12181 aagtgggctg ccgtttaggt agatttcgtt gacaatctca gccttctttt gtaggtaagg
    12241 attggtattg gtccattcga aaatttcttc aatctcagcc ttagtattca aggtagaaaa
    12301 gattgatgag taagatttag cgtggacaga ttccataaat tggatgttat tgaaaacagc
    12361 ttcctcatgt ggtgtacgga tgtctgcgcg aagggcttga accccagttt cagattgcat
    12421 agtgtcaaga agggttaaac caccaaaaac ttttccgacc aagtctttct ctttgttaga
    12481 tagctttctc cagtcatcca agtcgtttga taagggaata cgtgtatcga gccaaaattg
    12541 ctccgtcagt ttttcccaag ttgatttgtc gatgacatct tcgatggcat tccagttaat
    12601 ggctttgtag taagtttcca tttaaaatct ctttctgtgt ttagtattgc gaactcacaa
    12661 ttatttctac tttaccataa ttctatagga gtatcgcaca aaaagtcgga agcccgactt
    12721 ttaaaatgtt acataaatta tgttatgaca tagtagattt gattttatca gtgctgctta
    12781 gggaaaaata gtgtttctat gctagaaact aaatcacaca gctttcacat tggttggcgc
    12841 cgacttctcc accgtcatct gtaaaggtac ggacgtagta gatagacttg attcccttgt
    12901 taaaggcata gttacgaagg atggacaagt cacgtgtcgt ttgtttattt tccctcttcc
    12961 attcgtaaag gccttttgga atgtcactac gcatgaagag ggtgagtgaa agtccttgat
    13021 ccacgtgttc agtcgcagca gcgtaaacat cgatgacttt acgcatatcc atatcgtagg
    13081 cagaagtgta gtaaggaatg gtttctgtag acaagccagc agcagggtag tagattttac
    13141 caattttctt ttcctgacgt tcttcaatac gttgcgtaat cgggtggata gaagcagaaa
    13201 catcgttgat gtagctgata gaaccatttg gcgctacagc aaggcgattt tggtggtaaa
    13261 gaccatcctc ttgaaccttg tcgcgaagtt cagtccaatc agcaacacca gggataaaaa
    13321 catttttgaa gagttcttta acacggtctg atgttggaac aaattcacca gttacatact
    13381 tgtcaaagta acttccgtta gcatagtctg atttttcaaa gttgtggaag gtaataccac
    13441 gttcacgcgc aatattgttt gactctacca aggtccagta gttcataagc ataaagtaga
    13501 tgcttgtaaa ttcaacagac tcaggtgaac catattcaat gagttgttgg gcaaggtagc
    13561 tgtgcagtcc catggcaccg agaccaaagg tgtgggcttg gctatttcca tggtcaatcg
    13621 ttggtacagc tacgatatgt gaactatctg taacgaaagt aagggcacga accatagcac
    13681 ggatagaacg accaaaatca ggtgaagtca tcatgttaac cacgttggtt gaacccaggt
    13741 tacatgaaac atctgttccc atttgaagga attcttgagc atcgttgatc aagcttggtt
    13801 cttgaacttg aagaatctca gaacacaagt tactcatgat aatctttcca tcgacaggat
    13861 ttgcacggtt agccgtatct atgttgacta cataaggata gccagactct tgttgcaatt
    13921 tagagatttc agtttccaaa tcccgcgcct tgatttttgt cttgcgaata tttggatttg
    13981 cgaccaattc atcgtatttt tcagtaatgt cgatgtaatt gaatggcaca ccgtattctt
    14041 tttctacaga gtaagggctg aagaggtaca tttcttcatt tttacgagcc aattcgtaga
    14101 atttatcagg tactacaaca ccaagtgata gagtcttgac acgtactttt tcatcagcgt
    14161 tttctttctt agttgaaagg aaagcgatga tatctgggtg aaagacgttg aggtagacaa
    14221 caccggcacc ttgacgttgc cccaattggt tggagtaaga gaagctgtct tcaaaaagct
    14281 tcataacagg aacgacacct gaagcagctc cttcatagcc tttgataggt gcaccagctt
    14341 cacgaaggtt gctgagggta attcccacac caccaccaat acgtgaaagt tgaagagctg
    14401 agttgataga acgtccgata gagttcatat catccgtcac ttggattagg aaacaagata
    14461 ccaactcccc acgacgagca cgtccagcat tcaagaagga aggagtagca ggttggtagc
    14521 gttggtggat gatttcattg gcaatatcga ttgcaacagc ttcattccca tcagcgaaat
    14581 aaagggcgtt gaagaagaca cggtcttcca tattttcaag atagtattca ccgtcattag
    14641 tctttaaggc atattgattg taaaatttat aagctgccat gaatgacttg aattggaagt
    14701 tttggtcttt gataaattga gccaattctt ccaagaactc tggacggtat ttcttgataa
    14761 aggctgtttc gatgtagttg tgttcaatga ggtaattgat tttgtctttg attgaatcaa
    14821 aaaccatagt gtttggaact acattttctt taaagaaagc atccaaggct tccttatctt
    14881 tatgaagcat gatttgtcca ttaacaggac ggttaatttc gttattaaga cggaagtaag
    14941 tcacgtcttc aagatgtttt aatcccataa aatttccctt atctaattac aaaagaaagg
    15001 cttctaagtt agccctaaaa gcagtttctt ctggatgatg tactaagatt atgctaattg
    15061 tttcagtttt tctggttgga aacctgaaaa gacttcagtt ggtgtttgga taacaggagc
    15121 tgcgctaaaa ccgagctctt taacttgatc gacgtactca ggttgctcat caagattgat
    15181 ttcacgataa gagacattat tactgtccaa gaaacgcttg gtcattttac attggacaca
    15241 attgttttta gaataaacgg ttaccattgt gtaactcctc ttcaaaattt aatactatct
    15301 tagtatatca gaaaataaaa ttttgtcaat gatttaaact aaatatagtg gtgttctttt
    15361 gttgttttta tatataaaca caatatatag tgtttgtatt gtgaaatagc aatgattatc
    15421 ctataaattg tttttgaaga aactatatcc tgtgttttgt tatctaggat agaagatttt
    15481 cagcaagtta tctgaaagga aattgaagga aaatccagaa aatgcttgaa aaaaatccta
    15541 gaagatggta taatactaaa ttgtaagggt tatcacatat aactcaaaaa aagaaagaac
    15601 aaaaggagag tcaaactatg gcttctaaag atttccacgt agtggcagaa acaggtattc
    15661 acgcacgtcc agcaacattg ttggtacaaa ctgctagcaa atttgcttca gatatcactc
    15721 ttgagtacaa aggtaaatca gttaacctta aatcaattat gggtgttatg agtcttggtg
    15781 ttggtcaagg tgctgacgta actatctcag ctgaaggtgc agatgcagat gacgctatcg
    15841 ctgcaatctc agaaacaatg gaaaaagaag gattggcata agggaaatga cagaaatgct
    15901 taaaggaatc gcagcatctg acggtgttgc agttgcaaaa gcatatctac tcgttcagcc
    15961 ggatttgtca tttgagacta ttacagtcga agatacaaac gcagaagaag ctcgccttga
    16021 tgccgctcta caggcatcac aagacgagct ttctgttatt cgcgagaaag cagtaggtac
    16081 gctcggtgaa gaagcagctc aagtttttga tgctcactta atggttcttg ctgacccaga
    16141 aatgatcagc caaatcaagg aaactatccg tgcgaagaaa gtgaatgcag aagcaggtct
    16201 gaaagaagtt acagatatgt ttatcactat ctttgaaggc atggaagaca acccatacat
    16261 gcaagaacgc gcagcggata tccgcgacgt gacaaaacgt gtattggcaa accttcttgg
    16321 taaaaaattg ccaaacccag cttctatcaa tgaagaagtg attgtgattg cgcatgactt
    16381 gactccttca gatacagctc aattggacaa aaactttgta aaagcttttg taaccaacat
    16441 tggtggacgt acaagccact cagctatcat ggcacgtaca cttgaaattg ctgctgtatt
    16501 aggtacaaac aacatcactg aaatcgttaa agacggtgac atccttgctg ttaacgggat
    16561 cactggagaa gtgattatca acccgacaga tgaacaagcg gcagaattta aagcagctgg
    16621 tgaagcctat gcgaaacaaa aagctgaatg ggcacttttg aaagatgctc aaacagtgac
    16681 tgctgacggt aaacacttcg agttggctgc caatattggt actccaaaag acgttgaagg
    16741 tgttaacaac aacggtgcag aagctgttgg actttaccgt acagagttct tgtacatgga
    16801 ttctcaagac ttcccaactg aagatgagca gtatgaagca tacaaggctg ttcttgaagg
    16861 aatgaacggt aaacctgttg tcgttcgtac aatggatatc ggtggagata aggaacttcc
    16921 ttacttcgat atgcctcacg aaatgaaccc attccttgga ttccgtgctc ttcgtatctc
    16981 tatctctgag actggagatg ctatgttccg cacacaaatc cgtgctcttc ttcgtgcgtc
    17041 tgttcacggt caattgcgta tcatgttccc aatggttgcg ctcttgaaag aattccgtgc
    17101 agcgaaagca gtctttgatg aagaaaaagc aaaccttctt gctgaaggtg ttgcagttgc
    17161 ggataacatc caagttggta tcatgatcga gattcctgca gcggctatgc ttgcagacca
    17221 atttgctaaa gaagttgact tcttctcaat tggtacaaac gacttgatcc aatatacaat
    17281 ggcagcagac cgtatgaacg aacaagtttc atacctttac caaccataca acccatcaat
    17341 cctacgcttg attaacaatg tgatcaaagc agctcacgct gaaggtaaat gggctggtat
    17401 gtgtggtgag atggctggtg accaacaagc tgttccactt cttgtcggaa tgggcttgga
    17461 tgagttctct atgtcagcaa catctgtact tcgtacacgc agcttgatga agaaactcga
    17521 cacagctaag atggaagagt acgcaaaccg tgcccttaca gaatgctcaa caatggaaga
    17581 agttcttgaa cttcaaaaag aatacgttaa ttttgattaa tcgaaaagtc cctgcaactc
    17641 agttacaggg actttttttg atattttaaa aagaattttc aagaaaatct ttcttataaa
    17701 aagtccaacc ttgaaaaagt agtggtcaga acaaaaaata cttaaatggt tcataaaatt
    17761 cttgacaagt tggagattta ggagtaaact attaaccagt taagtaatag agaggagttt
    17821 ctgcaattta gaaatgaatt gcaactagaa atatcaaata gaaagagagt ttcgatgaaa
    17881 attaataaga aataccttgt tggttctgcg gcagctttga ttttaagtgt ttgttcttac
    17941 gagttgggac tgtatcaagc tagaacggtt aaggaaaata atcgtgtttc ctatatagat
    18001 ggaaaacaag cgacgcaaaa aacggagaat ttgactcctg atgaggttag caagcgtgaa
    18061 ggaatcaatg ctgagcaaat cgtcatcaag ataacagacc aaggctatgt cacttcacat
    18121 ggcgaccact atcattatta caatggtaag gttccttatg atgcgatttt cagtgaagaa
    18181 ttactcatga aagatccaaa ctataagcta aaagatgagg atattgttaa tgaggtcaag
    18241 ggtggatatg ttatcaaggt agatggaaaa tactatgttt accttaagga tgctgcccac
    18301 gcggataacg tccgtacaaa agaggaaatc aatcgacaaa aacaagaaca tagtcaacat
    18361 cgtgaaggtg ggactccaag aaacgatggt gctgttgcct tggcacgttc gcaaggacgc
    18421 tatactacag atgatggtta tatctttaat gcttctgata tcatagagga tactggtgat
    18481 gcttatatcg ttcctcatgg agatcattac cattacattc ctaagaatga gttatcagct
    18541 agcgagttag ctgctgcaga agccttccta tctggtcggg gaaatctgtc aaattcaaga
    18601 acctatcgcc gacaaaatag cgataacact tcaagaacaa actggctacc ttctgtaagc
    18661 aatccaggaa ctacaaatac taacacaagc aacaacagca acactaacag tcaagcaagt
    18721 caaagtaatg acattgatag tctcttgaaa cagctctaca aactgccttt gagtcaacgc
    18781 catgtagaat ctgatggcct tgttttcgac ccagcgcaaa tcacaagtcg aaccgccaga
    18841 ggtgtagctg tccctcatgg taaccattac cactttatcc cttatgaaca aatgtctgaa
    18901 ttggaaaaac gaattgctcg tattattccc cttcgttatc gttcaaacca ttgggtacca
    18961 gattcaagac cagaacaacc aagtccacaa tcgactccgg aacctagtcc aagtctgcaa
    19021 cctgcaccaa atcctcaacc agctccaagc aatccaattg atgagaaatt ggtcaaagaa
    19081 gctgttcgaa aagtaggcga tggttatgtc tttgaggaga atggagtttc tcgttatatc
    19141 ccagccaagg atctttcagc agaaacagca gcaggcattg atagcaaact ggccaagcag
    19201 gaaagtttat cgcataagct aggagctaag aaaactgacc tcccatctag tgatagagaa
    19261 ttttacaata agacttatga cttactagca agaattcacc aagatttact tgataataaa
    19321 ggtcgacaag ttgattttga ggctttggat aacctgttgg aacgactcaa ggatgtctca
    19381 agtgataaag tcaagttagt ggatgatatt cttgccttct tagctccgat tcgtcatcca
    19441 gaacgtttag g
//
LOCUS       DAVKQB010000043        18549 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKQB010000043 DAVKQB010000000
VERSION     DAVKQB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.043"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..985
                     /locus_tag="VW528_001569"
                     /pseudo
     CDS             1..985
                     /locus_tag="VW528_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1113..1316)
                     /locus_tag="VW528_001570"
     CDS             complement(1113..1316)
                     /locus_tag="VW528_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9912374.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(1309..2391)
                     /locus_tag="VW528_001571"
     CDS             complement(1309..2391)
                     /locus_tag="VW528_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9912375.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTIFETAEFENLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     IIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(2517..3299)
                     /locus_tag="VW528_001572"
     CDS             complement(2517..3299)
                     /locus_tag="VW528_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9912376.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(3299..4642)
                     /locus_tag="VW528_001573"
     CDS             complement(3299..4642)
                     /locus_tag="VW528_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9912377.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4754..6070)
                     /locus_tag="VW528_001574"
     CDS             complement(4754..6070)
                     /locus_tag="VW528_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9912378.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            6264..7490
                     /locus_tag="VW528_001575"
     CDS             6264..7490
                     /locus_tag="VW528_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9912379.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSASGKEKTDSLTQGMTANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGILGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            7689..9263
                     /locus_tag="VW528_001576"
     CDS             7689..9263
                     /locus_tag="VW528_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9912380.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            9527..10315
                     /gene="codY"
                     /locus_tag="VW528_001577"
     CDS             9527..10315
                     /gene="codY"
                     /locus_tag="VW528_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9912381.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            10315..10890
                     /locus_tag="VW528_001578"
     CDS             10315..10890
                     /locus_tag="VW528_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9912382.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFSPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10972..11057
                     /locus_tag="VW528_001579"
     tRNA            10972..11057
                     /locus_tag="VW528_001579"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11006..11008,aa:Leu,seq:aag)
     gene            11340..12470
                     /gene="ugpC"
                     /locus_tag="VW528_001580"
     CDS             11340..12470
                     /gene="ugpC"
                     /locus_tag="VW528_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9912383.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(12617..12763)
                     /locus_tag="VW528_001581"
     CDS             complement(12617..12763)
                     /locus_tag="VW528_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912384.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            12971..13729
                     /locus_tag="VW528_001582"
     CDS             12971..13729
                     /locus_tag="VW528_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9912385.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            13816..14328
                     /locus_tag="VW528_001583"
     CDS             13816..14328
                     /locus_tag="VW528_001583"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9912386.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            14510..15454
                     /locus_tag="VW528_001584"
     CDS             14510..15454
                     /locus_tag="VW528_001584"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9912387.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            15463..16140
                     /locus_tag="VW528_001585"
     CDS             15463..16140
                     /locus_tag="VW528_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9912388.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLIKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            16238..16996
                     /locus_tag="VW528_001586"
     CDS             16238..16996
                     /locus_tag="VW528_001586"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9912389.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            17034..18539
                     /gene="lytC"
                     /locus_tag="VW528_001587"
     CDS             17034..18539
                     /gene="lytC"
                     /locus_tag="VW528_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9912390.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMIQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
BASE COUNT         5647 a         3340 c         4103 g         5459 t
ORIGIN      
        1 acgtaatcca aaaacgagac ctgaagagag gacagaatta gaacgtcttc aagcagaaaa
       61 tgagtacctg agagcggaga atgccatcct aaaaaagtta agagaactcc gattgaagga
      121 ggaaaaagag aaagaagaaa gacagaaatt gttcaagaat taatgactga gttttcgtta
      181 gctcttcttc taaaatccat taaactagct tgttcgacct actactatca cttgaaacag
      241 ctagacaaaa cagataagga ccaagagctt aaagctgaaa ttcaatccat ttttatcgaa
      301 cacaagggaa attatgctta tcgctgtatt catttagaac taagaaatcg tgcttatttg
      361 gtaaatcata aaagagttca aggcttgatg aaagtactca atttacaagc taaaatgcga
      421 cagaaacgaa aataaaggag acgttggtaa gaaggcagag aatctcattc aaggccaatt
      481 tgaaggctct aaaacaatgg aacagtgcta cacagatgtg acagaatttg ccattccagc
      541 aagtactcaa aagctttact tatcaccagt tttagatggc tttaacagcg aaattatcgc
      601 ctataatctt tcttgttcgc ctaatttaga acaagtacaa acaatgttgg aacaggcatt
      661 caaagagaag cactacgaga atacgattct ccatagtgac caaggctggc aatatcaaca
      721 cgattcttat catcggttcc tagagagtaa gggaattcaa gcatctatgt cacgcaaggg
      781 aaacagccaa gacaacggta tgatggagtc cttctttggg attctgaaat ccgaaatgtt
      841 ttatggctat gagaaaacat ttaaatcact taaccaattg gaacaagcca ttatagacta
      901 tattgattac tacaacaaca aacgaattaa ggtaaaacta aaaggactta tccctgtgca
      961 atacagaact aaatccttcg gataaattaa ttgtctaact ttttgggggg cagttcattc
     1021 tacacccact attatgcgtt ttattgtggg aagattttcc tcattttcta gtgaaattga
     1081 actttttcta acatcttttg atagatgact tcttaatcaa ttatcatttc cattgttgtt
     1141 tcttggggtt tcatcccttg tctttgataa aagcggagag caccctcgtt atcattccag
     1201 acacttaggg ttagattatt gcagccaatt tctttagcat aatagcagaa aaatgacaat
     1261 aaaatagccc ctagatattt tgaaaggaaa gcataggggc tatatagttt aatcaaaata
     1321 aagcaaatct ttgctggtgc ttgtaaagag gtcgaagcca tccttggtaa caacaccgca
     1381 gtcttcaata cgaacaccga ctttaccagg gatatagata cctggttcaa cagagaagca
     1441 catgccttct tcgatgacca tgtcgtttcc ttccatgata gatgggaatt catggacatc
     1501 cataccgata ccatgcccga gacggtggtt gaagtactca ccataaccag ctttttcgat
     1561 gatctcacgg gcagcgcggt ccacttcatg agcagtcaca cctggcttga taaagtcaag
     1621 agcagcttgt tgggcttcaa gagtcaagtt gtaaatatct ttcttgaatt ggtctggttt
     1681 gccgacagcg actgtacgag tcatatctga cgcatagcca ttgaccagaa cccccaggtc
     1741 aaagaggaga agagcatcat tttcaacctt attagctgct ggaatgccgt gtggattcgc
     1801 agcattatca ccagtcaaga ccatggtatc aaagctcatt tcgtaacctt cacgtttcat
     1861 ggcaaagtca atttgtgcga tgatatctgt ctcagtctta tcaagagaaa tattgtcaaa
     1921 accaacatga acagccttgt cagcataaag acctgcaacc atcatttttt gtacttcatc
     1981 agctgatttg atgaggcgca tgcgttggat acgaggagtg aggttttcaa actcagcagt
     2041 ttcaaaaatt gttttcaaac catggtattt ggtcaagatg agattgtcaa actcaacagc
     2101 gacacgtttg aagtcaagtt gtggaagagc atgtttgatt ttttgccatg gattttcaga
     2161 atcgacatag cccactactg ggaaggaaac ggtgctactt gcacgttcta cttcaagagc
     2221 tgggacaaag aggagaggtt cctgatccgc taggacaaag aggaacattt ggcgttcatg
     2281 gggatcactg taaaaaccag tgaggtaatt gattgtgacg gggtcagata cgacagcgac
     2341 gtctagtttt tctgattcaa gatatgttag gatttgttgt aatttagaca tatgctacct
     2401 tctttctacc cctctatttt ggcaaaaaat gagagaaaat gcaagggaga agggtaaaag
     2461 aaaagacaaa aaactccgac aagataacgg agttctttga tatcattttc ctttgtttaa
     2521 gaaaagtcag ccttgctttt gacgtcactg tactcttcag cgatttcttg gctgaggata
     2581 tcctcatagg cagggagttg gatatcctga ccatatttct tcttgagggc agtagtaact
     2641 aggcgataag ccagattggc attacgagag aggataggcc cgtggaagta ggaaccaaag
     2701 acattcttat aatgaacccc ttcaccgacc ttttcttcgt tgtttccatt tccatagaca
     2761 acctgtccca gcggtttttg gtcatcagag aggaaggtac gaccttggtg attttcaaat
     2821 ccatagtagg tttcatcgaa atcttcattg tgaatcttga tgtcaccgat aaaacggtta
     2881 ttggtctggt tgagcgtgta gtgtcccatg acccctagcc cttcgatacg ttttcctgaa
     2941 gcttcaacat aatattgacc caatagttgg aaaccaccgc agatagccag aactacaccg
     3001 tcgttttgga tgtagttgtc aatgctctct tttttagcag gtaggtcgtc tgcaatgata
     3061 ctttgttcaa agtcttgacc accaccgaaa aaggcgatgt cgtagtgatt ttcatcaaag
     3121 tcatcatgga gagaaacgat gtcaacggtc acatgggctc ccagtttttc agccacatac
     3181 ttgagcatga ggatgtttcc attgtccccg taggtattca tgagatttcc gtagaggtgg
     3241 gcaatgttga gctgataggg gtaattgcca tcttttgagg aaagtgaagt ataaaccatt
     3301 agttcatctc ctttctaaca atctgacgac tagccagcag ttcacgaaat tccagcatgg
     3361 cagtataagt tgccagaata taggcatgct tgcagtcttg attctcaatg gtcttgagaa
     3421 cttgctccag attactcgtt tcagtgattt tctcagctgg atagccagtc actcggaggc
     3481 gacgagcgat ttcagaatga cgaacaccgc cagcgttgat ttcaggaatg tccatgtcag
     3541 tgatttgttc aaagtctgca tcccagatcc agctagtgtc aattccatct gcatagttgg
     3601 cattaaggag gacagatagg ctaaatggat aaggtgctag tttgatcatt tcgatagctt
     3661 gggttgcacc gactggattt ttaatcaaga caagggtaca ttccttgtca ccgatatgaa
     3721 aggtttcttg gcgtccaaag acagcacggc tcttgtcaaa tccctgtttg atgagttgcg
     3781 aatcggcacc taggaaacgg gcgatggcca cagcagctag ggcgttatag atattataga
     3841 gcccgccgat ttggataccg tattcttggc cgtctatgac aaagcgagag cgattgttgg
     3901 tcaactcaac cagttttgtc aaacgatagt cgagatcagg acgtttacat ccacaacctt
     3961 cacagatata ggcacccaag tttgcatagg tattatgctc atatttgagg atgccttggc
     4021 agtcaggaca gagaatccct tcggtattgt agtgagccag ttgggctggt cccttttcca
     4081 agtcaaaacc aaaatactct atagggtttg gaatagttgg cttgtagaaa agtggactgt
     4141 ctccgttaag gagaacagta gcagttggaa ctttccgaat ggcatccaat atcatgttat
     4201 aggtagtata gatttcaccg aaacggtcca tctggtcacg gaagatatta gtaatgacaa
     4261 aaagactagg ctggatatag tcacagatac gagatagact ggcttcgtca atttcgagga
     4321 cggcaatatt tttcccagtt ttagaagatt tggctgttag gaaggttgtt gcaatccctg
     4381 taatcatgtt ggcaccgctt gggttggtta gaacttgacc ataaacctct tttaaaatgc
     4441 cgacagtgag ggcagttgtc agggtttttc catttgttcc agtgacaacg acaatctcgt
     4501 agttcttagc taggttttgt aaaatatctt tatcaaattg aagggcgact ttccctggga
     4561 gcgtacttcc acgtccaaga cggcttaaaa cgaagtggga agaacgccca gcaagaaggc
     4621 ccaaagtagt ttttaagttc atgtttctat tatatcacaa aagtatatca caaaagaggg
     4681 aaaagacaga tttggtgttt cgtagaagta aaaacagccc aaagagggct gtcagttaag
     4741 atgagatttc aacttaaatc gcaaacaagt cattcaagtt ctcagccaag gttgggtgag
     4801 tgaagatttg ttttgtgaag taagtgtaag gaatcttgtt gtccatagca acagtaataa
     4861 tgttgatgat ttcttgagaa ccttctgaga agatgcttgc accaagaatt tcttttgttt
     4921 cagtattaac aacagctttg aaagctccgc gaaggtctcc atttacgtga ccacgaggca
     4981 tggctgcaac agggatttct ttcactgcgt atggaagttt caaatcagct gcttggcttt
     5041 cagtcaaacc aacttgtgaa agtgcaggtg tgatgaacat agtatttggc acattgagac
     5101 ggtcctcaag tgtgtagctg ccatctccag caaggtagct gtaaacaaca cggaagtcat
     5161 caagtgaaat gtaagtaaat tgaagaccac cgttgacatc tccaactgca aagacaccag
     5221 gaacgtttgt ttgacagtgt ttatctactt taatagcgcc acgttcagtt agttcaatat
     5281 ctgtattttc aagttgaagt ggttctacat ttggtttgcg tccagttgcg tagagaaggg
     5341 cgtcgaaacg gtaagtttcg tcttcagtta cgacaagcac ttggtcaccg tcgtttttaa
     5401 tttcagtagt atggatattt tgaagcaatt caataccgtc ttcttccatg tattgtttag
     5461 caagagctgc gatggaaggt tctgcacgag gtaggaatgt atccaaggca tctaggactg
     5521 taaccttgct tcctagttta ttgtaaaggc cagcaaattc aagaccgata tttccgccac
     5581 caaggactcc aagtttttca ggcaatttat ccaagctttg gatacctgtt gagtcaaaga
     5641 cgtttttgct tgtagcaagt ccagggattg gcaagacgtt tgaaacagca ccagtgttga
     5701 tgacgatagt ttcagcagtc aattcttgtt tttcatcacc agcttggatt tcgatgactt
     5761 tatttgaaag gaagtgagct tcagcatcaa agatatcgac gccagtacca gcaacagtcg
     5821 tatagttttt accgttgagg cgaccagtga tcgtgttttt ggtagcaatg acttcttcaa
     5881 aagacaagtc tttctcagca gcaaccagca aagttttagt tgggatacaa ccgatgttga
     5941 tacaagttcc accgtacata gctttgctac gttcaacgag ggcaactttt ttgccagctg
     6001 aagccaattt accagctagt gttttaccag ctttaccaaa tccgataacg attaaatcat
     6061 aagttaacat ttagaattcc tcctattcga attacattct agcacaagaa aaaaactttt
     6121 gcacaattct gctacgaaaa agacagaaaa atatgaaaac gcttttcagg caaaaaacta
     6181 gtaatttttt gacaagcttg ataaactaaa atcctatcta tttttatata agatagaata
     6241 aatgttcgaa aataggaggt tttatgaaat cgaatcgtta tattattgcc tttgctgggg
     6301 tcattttaca cttaatgctg ggttcgactt atgcctggag tgtttatcgt aaccctatta
     6361 ttgaaaaaac gggatgggat caggcttctg ttgccttcgc ctttagtcta gcaatctttt
     6421 gtttgggctt atcggctgca tttatggggc gtttggtaga aaaatttggt ccgagagtca
     6481 tggggagtct atctgctttt ctatacgcag gtggaaatat cttaacagga tttgcaatag
     6541 accgtcagga actgtggttg ttgtatctag cttatggcat tttaggtggg cttggtttgg
     6601 gagcaggcta tattacccct gtgtcaacga ttataaaatg gtttcctgat aaacgtggtc
     6661 tcgcaacagg tttagcgatt atggggtttg gttttgcttc tttattgact agtcccatag
     6721 cgcaacatct catcgcaggg gtagggcttg tagaaacttt ttatatttta ggtgcaagtt
     6781 actttattat catgctccta gcttcacaat tcattaagcg tccaaatgag caagagcttg
     6841 caattttatc tgcttcaggg aaagaaaaaa cagactctct gacgcaaggg atgactgcaa
     6901 atcaggctct aaaaagcaat cggttttata tgctttggat tattttcttt atcaacatag
     6961 cttgtggttt aggcttaatt tcagcggcat cgccaatggc acaggagatg gctggcttgt
     7021 ctacaagtca tgcagcagta atggtgggta ttttggggat tttcaatgga tttggtcgct
     7081 tgctctgggc gagtttgtct gactatatcg gtcgccctct aacctttagt atattactgc
     7141 ttgtcaatct tttcttttct ctctcacttt ggctctttac agattccgtt ttatttgtag
     7201 ttgctatgtc tattttgatg acttgctatg gagctggttt ttctttgatt ccagcttatc
     7261 tcagtgatat ttttggaacc aaggaattgg ccgctctgca tggttatatt ttaacagctt
     7321 gggcaatggc tggtttagcg ggacctattt tattagcaga gacttataaa atggctcatt
     7381 cgtacacaca aaccttgttc gtttttctca ttttatacag tatcgccttg gctttgtctt
     7441 attatctagg tcgttcaatc aaaaaagaaa gtcaaaaacc gcttacatga ttttctgtag
     7501 aaatggggct atcttttgct ctggtcttgt gttatactag ataggttgca aagaaaacag
     7561 tacttttctt ttgtggaaaa gaagcaacac gattttatat ctagttatat gaaaatacgt
     7621 cataaaaaga aaagtataaa ctaagcccta tagggtggct tcgcaccacc tttagaaaga
     7681 agaataacgt gaaatttaat gaattaaact tgtctgctga tttgctagca gaaattgaaa
     7741 aagctggttt tgtagaagct agtcctatcc aagaacaaac tattcctttg gcccttgaag
     7801 gcaaggatgt tatcggtcaa gctcagactg gtacaggaaa aactgcagcc tttggcttgc
     7861 ctacccttga aaaaatccgt acagaagaag cgactatcca agctttggtc atcgctccaa
     7921 ctcgtgaact agctgtccaa agtcaagaag aactcttccg ctttggtcgt agtaagggag
     7981 tcaaagtccg ttcagtatat ggcggatcaa gcattgaaaa acaaattaag gctcttaaat
     8041 ctggtgccca tatcgtggtg ggaactccag gtcgcctctt ggacttgatt aaacgcaagg
     8101 ccttgaaatt acaagacatt gaaaccctta tccttgacga agcggatgaa atgcttaaca
     8161 tgggcttcct tgaagacatc gaagccatta tttcccgtgt acctgagaac cgtcaaactt
     8221 tgcttttctc agcaactatg ccagatgcca tcaaacgtat cggtgttcag tttatgaaag
     8281 cccctgaaca tgtcaagatt gcggctaagg aattgacaac agaattggtt gaccagtact
     8341 atatccgtgt taaggaacaa gaaaaatttg acaccatgac tcgtctcatg gatgtggcac
     8401 aaccagaact cgctattgta tttggtcgta ccaaacgccg tgtggatgaa ttgactcgtg
     8461 gtttgaaaat tcgtggcttc cgtgcagaag gaattcatgg cgacctagac caaaacaaac
     8521 gtcttcgtgt ccttcgtgac tttaaaaatg gcaatcttga tgttttggtt gcgacagacg
     8581 ttgcagcgcg tggtttggat atttcaggtg tgacccatgt ctacaactac gatattccac
     8641 aagatcctga gagttatgtt caccgtatcg gtcgtacagg tcgtgctggt aagtcaggtc
     8701 aatctattac ttttgttgct ccaaacgaaa tgggttacct tcaaatcatt gaaaacttga
     8761 ctaagaaacg catgaaaggt ctcaaacctg caagtgtaga agaatccttc caatcaaaaa
     8821 aacaggtagc tctcaagaaa atcgaacgtg attttgcaga tgaaaccatt cgtgccaact
     8881 ttgagaaatt tggtaaggat gctcgcaaat tggctgctga gtttactcca gaagaattgg
     8941 caatgtatat cttgagtctg acagtccaag acccagatag ccttccagaa gtggagattg
     9001 cacgtgaaaa accactaccg tttaaaccat caggtaatgg tttcggtggt aaagctaagg
     9061 gaggtcgtgg aggccgtcgt ggggacgacc gtcgagagcg tgatcgccgt ggcaatggtc
     9121 gccgtgatga gttcaaaaaa ggaagtcgtg gcaacgatcg ttttgataag gaaaaacgtt
     9181 accgtaagga taataaaaaa ccacgcaata ctttaagcga aaagcaaaca ggctttgtta
     9241 ttcgtaacaa gggcgataaa taagaaaaag cggttcaaaa tgaatcgctt ttttatataa
     9301 ggagaccgaa tgtgaaaaga caaataaaat tattatatta tcttttataa tgttatgata
     9361 cacaaaaata ggcaacttgt caatagaaaa ggaacaattt aattaaaaat attctggttt
     9421 ttaattaaat tgtaagtcaa tgcaagtcaa taaggaattt tcagataatt attgaaaaag
     9481 agacgttttt atgttataat gatagcgatt aaattcgagg tgaaaaatgg cacatttatt
     9541 agaaaaaact agaaaaatta catcaatttt gaaacgctca gaggagcagt tgcaggatga
     9601 gcttccttac aacgctatta cgcgtcagtt agcggatatt attcattgca atgcctgcat
     9661 tatcaatagt aagggacgtc tgcttggcta ttttatgcgt tataaaacaa atacagatcg
     9721 cgtagagcaa ttcttccaaa ctaagatttt cccagatgac tacgttcaag gggctaatat
     9781 gatttacgaa acagaagcaa acttgcctgt tgagcatgat atgagtattt tccctgttga
     9841 gagtagagat gattttccag atggcttgac gactattgca ccgattcatg tatcggggat
     9901 tcgccttggt tctttgatta tttggcgtaa tgataaaaaa ttcgaagatg aggacttggt
     9961 tcttgttgag attgccagta ccgttgttgg gattcagctt cttaacttcc aacgtgaaga
    10021 agatgagaaa aatattcgtc gtcgtactgc tgtcaccatg gcggtcaata ccctttctta
    10081 ctccgaactc cgtgctgttt cagcaatttt aggggaatta aatggaaatg aagggaagtt
    10141 gactgcgtca gtgattgcag atcgtatcgg aatcactcgc tctgtgattg tcaatgctct
    10201 tcgtaaactt gagtctgcgg ggattattga aagtcgctca cttggaatga agggaaccta
    10261 tcttaaggtc ttgatttcag atatttttga agaagtgaag aaaagagatt actaatgaca
    10321 aaggctttaa tttcgattga ttatacagaa gattttgttg ctgatagtgg gaaactaaca
    10381 gcaggtgctc cagctcaggc gatttcggat gccatcagca aggtaactcg attagctttt
    10441 gaacgaggag attatatctt ctttactatt gatgctcatg aagaaaacga ttgtttccat
    10501 ccggaaagta agttattttc tcctcataat ctgattggga cgagtggacg gaatttatat
    10561 ggagatttgg ggatctttta tcaagagcat ggttcagaca gtcgtgtctt ttggatggat
    10621 aaacgccatt actcagcttt ttcagggact gacctagata ttcgtttgag agagcgtaga
    10681 gtgtctaccg ttatcttaac aggtgtcttg acggatatct gtgtcctaca tacagctata
    10741 gatgcctata atctaggata tgacatcgag attgttaaac cagctgttgc ttccatctgg
    10801 cctgaaaatc atcaatttgc cctaggtcat ttcaaaaata cactcggagc taagttagta
    10861 gatgaaaatc taaatgaact ttctgagtaa cctggttggt gaaaatgaaa aaatttgaaa
    10921 aaaagtgttg acaagcatcc caaaagttga tatactagta aagtaatcga tgcggggatg
    10981 gcggaattgg cagacgcgca ggactaagga tcctgtgacc gctttaggtc gtgagggttc
    11041 aagtccctct ctccgcatag gaaaagagtt agctaaagct agctcttttg tatttctcta
    11101 aaaaattaga atccagtcaa atgtagtgga gaatcagcga aaatatggga aaactgatcc
    11161 ttgtttctgc ttttgtcata gaagaataat aaagattatc ttcttcaaat tctccgatat
    11221 tctctaaagt tttgtgcaag ttgcacagaa cttttttatt tttttggtca tcttgccata
    11281 gaaatataaa gcgttttcat ctataatata attatcaaaa gacaaaagga gttcacctca
    11341 tggtagaatt gaatcttaaa aatatttaca aaaaatatcc aaacagcgaa cactattcag
    11401 ttgaagattt caacttgaac atcaaagata aagaatttat cgttttcgta ggaccttcag
    11461 gatgtggtaa atcaactaca ctccgtatga ttgctggtct tgaagacatt acagaaggta
    11521 ctgcatctat cgatggcgtg gttgtcaacg acgtagctcc aaaagaccgt gatatcgcca
    11581 tggtattcca aaactacgct ctttacccac acatgactgt ttatgacaac atggctttcg
    11641 gtttgaaatt gcgtaaatac agcaaagaag acattaacaa acgtgttcaa gaagcagctg
    11701 aaatacttgg attgaaagaa ttcttggaac gtaaaccagc tgacctttca ggtggtcaac
    11761 gtcaacgtgt tgccatgggg cgtgcgattg tccgtgatgc gaaagtattc ttgatggacg
    11821 aacctttgtc aaacttggat gccaaacttc gtgtatcaat gcgtgctgaa atcgctaaaa
    11881 ttcaccgtcg tatcggagct acaactatct atgtaactca cgaccaaaca gaagcgatga
    11941 cacttgcaga ccgtatcgtt attatgtcag ctactaagaa ccctgctggt acaggtacta
    12001 tcggacgtgt agaacaaatc ggtactcctc aagaagttta caaaaatcca gttaacaaat
    12061 tcgttgcagg attcatcgga agcccagcta tgaacttcat caccgtgaaa ttggttggta
    12121 gcgaaattgt ttctgacggt ttccgtttga aagtgccaga aggagcattg aaagttcttc
    12181 gtgaaaaagg ctacgaagga aaagaattga tctttggtat ccgtccagaa gacgtgaatg
    12241 cagaacctgc tttccttgaa acattcccag actgtgttgt aaaagcgact atctctgtat
    12301 cagaactgct tggttcagaa tctcaccttt actgccaagt tggtaaagac gagtttgttg
    12361 caaaagttga tgctcgtgac tacttgcaaa caggtgcaac agttgagctt ggatttgact
    12421 tgaacaaagc acacttcttc gatgtagaaa ctgaaaaaac aatctactaa aataaataaa
    12481 attcaaagca ctacaagaaa agatatctct ttatcaattg tagtggagag atatcagtta
    12541 atctagggag agaaacaaaa tgcttctctc ctttttgcta gggaagtcat attatgcatc
    12601 tatattgtga tgctctttaa tactcttcga aaatctcttc aaaccacgtc aacgtcgcct
    12661 tgccgtacgt atgattactg atttcgtcag ttttatctgc aacctcaaag atgtactttg
    12721 agcagcttac ggctagtttc ctagtttgct ctttgatttc cattgagtat tatttgtggg
    12781 taccatctac aagtgaagct atatgcgtaa actacgtgag caattgaatt cgaactagag
    12841 aggtaataat aaatttatgc tatagttatg gtgacttgta tgcttttgat tctagtttat
    12901 caaataatag attagaattg tcagataata tcattttgtg ttataatgaa gaaaaaacag
    12961 aggtgttcaa atgtcagaag caggtcataa gtttttagca aaattgggga aaaaacgctt
    13021 acgtccaggt ggaaagcgtg ccacagattg gttaattgca gaaggaggat tttcaaaaga
    13081 aaagagaata ctagaggttg cgtgtaatag gggaactaca gcaattgagt tggcacagcg
    13141 ttttggttgc aagataactg ctgttgatat ggatgctcaa gctttagaag tggctaaaaa
    13201 atctgctgga acggcaggtg ttgctcattt aatcagtttt gaaagagcaa atgcaatgaa
    13261 acttccttat caagatgcta gttttgatat tgttataaat gaagctatgc tgactatgca
    13321 agccgatcaa gctaagaaaa aatgtgtaat ggaatatcta agggtattaa aacctggagg
    13381 tcttctcttg acacatgatg tgcttcttaa ggaagctaaa gagtctatca gacaggaatt
    13441 atcacaagca attcatgtaa atgtaggtcc tttaactcaa gatggttggg aacaggtgat
    13501 gatagaatca ggttattgtg atgtgaaagc attgactggt gaaatgacat taatgaaatt
    13561 atcgggtatg atttatgacg aaggtttgct aggaactttg aaaatttgtg taaatgcttg
    13621 taaaaaggag aatagaaagc agtttttaac tatgtataaa atgtttgcta agaataaaca
    13681 gaaattgggc tttattgcga tggctagtta taaatcgtca aaacgttaga taattattga
    13741 agttaacttt tccttttttc tttcttaaaa aatatgctat aatagagagt aaaaaacttt
    13801 gaaagaaaga aaaagatgaa tttaaaagat tacattgcaa caattgaaaa ttatccaaag
    13861 gaaggcatta ccttccgtga tattagtcct ttgatggctg atggaaatgc ttatagctac
    13921 gctgttcgtg aaatcgttca gtatgctact gacaagaaag tcgacatgat cgtgggacct
    13981 gaagctcgtg gatttatcgt gggttgtcca gttgcctttg agttgggaat tggttttgcg
    14041 cctgttcgta agccaggtaa attgccacgc gaagttattt ctgctgacta tgaaaaagag
    14101 tacggtgtcg ataccttgac tatgcacgcg gatgccatta agccaggtca acgtgttctt
    14161 attgtagatg accttttggc gacaggtgga actgttaagg caactatcga gatgattgaa
    14221 aaacttggtg gtgttatggc aggttgtgcc ttccttgttg aattggatga attgaacggc
    14281 cgtgaaaaaa ttggtgacta cgactacaaa gttcttatgc attattaatg aaaacagtcc
    14341 ctagggctgt tttctctaca ctaggatata aaaatagact ataactagtt agagaaaaac
    14401 tataattgaa aactatatct tcttgcagta taataaaagg actaagtgtt tgagatttgt
    14461 cttcaaacat atgcaattat tcctgaaaga gtacagttag gagagggtta tgccgattcg
    14521 aattgataaa aaattgccag ctgttgagat tttacggaca gagaatatct ttgtcatgga
    14581 tgatcaacgt gctgcccacc aagatatccg tcctttgaag attttaattt taaatctcat
    14641 gccacagaaa atggtcacag agacccagtt gttgcgccac ttggctaata cacccctaca
    14701 actggatatt gattttctct atatggagag ccaccgttct aaaacaactc gttcagagca
    14761 catggagacc ttctataaaa cttttcctga agtcaaggat gagtattttg atgggatgat
    14821 catcacgggt gctccagttg agcatttacc atttgaggaa gtggactatt gggaggaatt
    14881 tagacagatg cttgagtggt ctaagactca tgtctattcg acccttcata tctgttgggg
    14941 ggctcaggct gggctttatc tgcgctatgg tgtagaaaaa taccagatgg acagtaagct
    15001 atcaggtatt tatcctcagg acaccctaaa agagggtcac cttctattta gaggctttga
    15061 tgatagctat gtatcccctc attcacggca cacggagatt tctaaggaag aggtcttaaa
    15121 caagaccaat ctcgagattt tatcagaagg acctcaggtt ggggtttcta ttttggccag
    15181 tcgtgattta cgagaaattt atagttttgg tcatttggag tatgaccgtg ataccttggc
    15241 aaaagagtat tttcgagatc gtgatgcagg tttcgaccca catattccag aaaattactt
    15301 taaagatgat gatgttaatc aggtaccttg tctttgttgg tcttcatctg cagccctctt
    15361 tttcagtaat tgggtaaacc atgcggtcta tcaggagacg ccttttgatt ggagaaagat
    15421 agaagatgat gcatctgcat atgggtattt ataagaggaa ttatgacata tttagacgct
    15481 tttaaatcag gtaccttggt tttaccgagt gccctgctct tgcattttaa ggaactcttt
    15541 ccttctagcg acgattttct ggtttggcaa tttttctatt tgcaaaatac gacaggctta
    15601 gaagaaatgt cgccaagcca gattgctgaa aggattggca aggaaatttc ggatgtcaac
    15661 cagtccattt ctaatctgac ggaaagggga ctgctccagt atcgtactat cgaattaaat
    15721 ggcgaaattg aattgctctt tgatgctagt ttggccttgg aacgtttgga tgacctgttt
    15781 ggagcagttc gttcaagttc agaccagcta acacctcaaa accagctcaa ggatttggtg
    15841 gaaaccttcc agcaggagtt gggacgattg ttgacgcctt ttgagattga ggatttgatc
    15901 aagacactaa aggaagatgg aaccagtgct gacttgatta aggaggctct tcgtgaagct
    15961 gttttgaatg gaaaaccaaa ctggaagtac attcaggcga ttttgagaaa ctggcgccat
    16021 gaaggaatca agagtgtggc tcaaattgag gccaagagag cagaaagaga agcaagcaat
    16081 cctcagttga cacaggtatc tgcagatttc agaaatgcca tggatctctg gaaggattaa
    16141 tccatgcaag taggcttgaa atccgagtaa gatttgcaag ctgtgtataa ttgtgataga
    16201 ataaatagaa aataaattga aaaaagaggt atgtgaaatg tcacgtaaac catttatcgc
    16261 tggtaactgg aaaatgaaca aaaatccaga agaagctaaa gcattcgttg aagcagttgc
    16321 atcaaaactt ccttcatcag atcttgttga agcaggtatc gctgctccag ctcttgattt
    16381 gacaactgtt cttgctgttg caaaaggctc aaaccttaaa gttgctgctc aaaactgcta
    16441 ctttgaaaat gcaggtgctt tcactggtga aactagccca caagttttga aagaaatcgg
    16501 tactgactac gttgttatcg gtcactcaga acgccgtgac tacttccatg aaactgatga
    16561 agatatcaac aaaaaagcaa aagcaatctt tgcgaacggt atgcttccaa tcatctgttg
    16621 tggtgaatca cttgaaactt acgaagctgg taaagctgct gaattcgtag gtgctcaagt
    16681 atctgctgca ttggctggat tgactgctga acaagttgct gcctcagtta tcgcttatga
    16741 gccaatctgg gctatcggta ctggtaaatc agcttcacaa gatgatgcac aaaaaatgtg
    16801 taaagttgtt cgtgacgttg tagctgctga ctttggtcaa gaagtcgcag acaaagttcg
    16861 tgttcaatac ggtggttctg ttaaacctga aaatgttgct tcatacatgg cttgcccaga
    16921 cgttgacggt gcccttgtag gtggtgcgtc acttgaagct gaaagcttct tggctttgct
    16981 tgactttgta aaataatcag taagtagcaa aagctaggtg gaacagcatt cagatgtctg
    17041 ttacattttt tataggagag gaaagattga aaataaaaat tggattagca agtatctgtt
    17101 tactaggctt ggcaactagt catgtcgctg caaatgaaac tgaagtagca aaaacttcgc
    17161 aggatacaac gacagcttca agtagttcag agcaaaatca gtcttctaat aaaacgcaaa
    17221 cgagcgcaga agtacagact aatgctgctg cccactggga tggggattat tatgtaaagg
    17281 atgatggttc taaagctcaa agtgaatgga tttttgacaa ctactataag gcttggtttt
    17341 atattaattc agatggtcgt tactcgcaga atgaatggca tggaaattac tacctgaaat
    17401 caggtggata tatggcccaa aacgagtgga tctatgacag taattacaag agttggtttt
    17461 atctcaagtc agatggggct tatgctcatc aagaatggca attgattgga aataagtggt
    17521 actacttcaa gaagtggggt tacatggcta aaagccaatg gcaaggaagt tatttcttga
    17581 atggtcaagg agctatgata caaaatgaat ggctctatga tccagcctat tctgcttatt
    17641 tttatctaaa atccgatgga acttatgcta accaagagtg gcaaaaagtg ggcggcaaat
    17701 ggtactattt caagaagtgg ggctatatgg ctcggaatga gtggcaaggc aactactatt
    17761 tgactggaag tggtgccatg gcgactgacg aagtgattat ggatggtgct cgctatatct
    17821 ttgcggcctc tggtgagctc aaagaaaaga aagatttgaa tgtcggctgg gttcacagag
    17881 atggtaaacg ctatttcttt aataatagag aagaacaagt gggaaccgaa catgctaaga
    17941 aaatcattga tattagtgag cacaatggtc gtatcaatga ttggaaaaag gttattgatg
    18001 agaacgaggt ggatggtgtc attgttcgtc taggttatag cggtaaagaa gacaaggaat
    18061 tggcgcataa cattaaggag ttaaaccgtc tgggaattcc ttatggtgtc tatctctata
    18121 cctatgctga aaatgagacc gatgctgaga atgacgctaa acagaccatt gaacttataa
    18181 agaaatacaa tatgaacctg tcttacccta tctattatga tgttgagaac tgggaatatg
    18241 taaataagag caagagagct ccaagtgata cagacacttg ggttaaaatc atcaacaagt
    18301 acatggacac gatgaagcag gcgggttatc aaaatgtata tgtctatagc tatcgtagtt
    18361 tattacagac gcgtttaaaa cacccagata ttttaaaaca tgtaaactgg gtagcggcct
    18421 atacgaatgc tttagaatgg gaaaaccctt attattcagg agaaaaaggt tggcaatata
    18481 cctcttctga atacatgaaa ggaatccaag ggcgcgtaga tgtcagcgtt tggtattaag
    18541 cgatgattt
//
LOCUS       DAVKQB010000044        18071 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKQB010000044 DAVKQB010000000
VERSION     DAVKQB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18071
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.044"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(85..1020)
                     /locus_tag="VW528_001588"
     CDS             complement(85..1020)
                     /locus_tag="VW528_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9912391.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1017..1877)
                     /locus_tag="VW528_001589"
     CDS             complement(1017..1877)
                     /locus_tag="VW528_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9912392.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            1985..4147
                     /locus_tag="VW528_001590"
     CDS             1985..4147
                     /locus_tag="VW528_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9912393.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYFYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4239..5498
                     /locus_tag="VW528_001591"
     CDS             4239..5498
                     /locus_tag="VW528_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9912394.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5512..6378
                     /locus_tag="VW528_001592"
     CDS             5512..6378
                     /locus_tag="VW528_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9912395.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTRWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6392..7225
                     /locus_tag="VW528_001593"
     CDS             6392..7225
                     /locus_tag="VW528_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9912396.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKLTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7226..7306
                     /locus_tag="VW528_001594"
                     /pseudo
     CDS             7226..7306
                     /locus_tag="VW528_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7583..9025
                     /gene="gtfA"
                     /locus_tag="VW528_001595"
     CDS             7583..9025
                     /gene="gtfA"
                     /locus_tag="VW528_001595"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9912397.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRSVVKALLNLFSFRNRSEAFDLEGTIEIETPTVHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9163..10356
                     /locus_tag="VW528_001596"
     CDS             9163..10356
                     /locus_tag="VW528_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9912398.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            11043..13022
                     /locus_tag="VW528_001597"
     CDS             11043..13022
                     /locus_tag="VW528_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9912399.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13089..14585
                     /locus_tag="VW528_001598"
     CDS             13089..14585
                     /locus_tag="VW528_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9912400.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14585..15511
                     /locus_tag="VW528_001599"
     CDS             14585..15511
                     /locus_tag="VW528_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9912401.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15520..16587
                     /locus_tag="VW528_001600"
     CDS             15520..16587
                     /locus_tag="VW528_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912402.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16598..17524
                     /locus_tag="VW528_001601"
     CDS             16598..17524
                     /locus_tag="VW528_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912403.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQTELARYQKGLN"
     gene            complement(17599..>18071)
                     /locus_tag="VW528_001602"
     CDS             complement(17599..>18071)
                     /locus_tag="VW528_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9912404.1"
                     /translation="KAIKHYWKLIQQDSRKLSDKQFYRPTFRMHLTNKEILNKLLSYS
                     EDLKHHYQLYQLLLFHFQNKEPEKFFELIEDNLKQVHPIFQTVFKTFLKDKEKIINAL
                     QLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         5315 a         3205 c         3798 g         5753 t
ORIGIN      
        1 caataaaact tcagatccgt aaaacttaat tgaattacta atctgaagtt atttttatat
       61 cgtttatatt ataattgtgt tattttactt ccaactattg agagaaattt cgccactatt
      121 tagccagatt tcttttccgt tatcacattg aactaaaagt tttccatttt ctgagatgtc
      181 tttagcaagt cccttgtagt ctttttgctc tagtgtgaaa gtgacttctt ttcctagaat
      241 gaatgactgt tttttgtata ggtataatag ctcttctgct ggtgtttcga agaaagcacg
      301 ccagatttct atgatcaatt cattccttgt tataggagct gtagctttaa ataagctggc
      361 agctttttct tttaattcct gagggaagtc tttaatagtg aaattgatac ctactccaat
      421 aatgatatct gtgactaagc cagtttctac agaggtcatt gcttcagtaa ggattcctcc
      481 aattttatga ttgtttagat agatatcatt gacccatttt atgtcgacat ctattaaagt
      541 taggttctta atggctttgt agacagctcc agctacaagt agtgtgtagg atggtaattt
      601 gtcatagggg agatttggtt taagatggag tgtcatataa ataccacctt gtggtgagta
      661 gaaggaacgt tgaaaacggc ctcggcctgc tgtttgatag gaagctagat agagggtatt
      721 tgcttcatgg cctaaatcaa ttgcttcttt tgcatctagt tgtgttgatt ttgtttcggg
      781 tttaaagctg actttaattg gaagattttc ttctagaatc tctggaagaa taaggtcacc
      841 attcatcagt ttatatcctc tatttttgat actatcaatt tcaatgcctt cttgttctag
      901 tcgcttgatg gctttccaaa ttgctgttcg gcttagggat agtttttctg cgattttttc
      961 tccgctgata tagtcggttt ctttagatag gatttggtag acagcttggt aggatttcat
     1021 aatgttgcct ttcttactag ttggtattga gagtattctt ttcttgtatg acttggagtc
     1081 tgattaaagt attgtttata agctttcgaa aaatggagtg gatctgaaaa acctaccgag
     1141 tatgcaatta ccttgatgga ctcttgggta ttttcgagaa gttgtctagc tcggtgcatt
     1201 cgaacgtaga gtaggtattc tttgggtgat aaggtattaa attctttgaa tacgcttgat
     1261 aagtagcttc tgtgaacgga tagttctttt gctaaatctt gaattgtaag tgattgagga
     1321 tagtggctat caattaatcg tttgcattca agatagagtt ggtgggttga tgaaatattc
     1381 ttttttttct gattgggagc aatagttccc agatgaaaca tcagttcatg aagttgtccc
     1441 atgatatgga gttgagctaa ttcacttgat tttgtaatct gagcgaagcg gacaatgtct
     1501 gagatgagtt ttgcagtagt ctgggtatga caagtttcag attggatgag ataggattga
     1561 tcagaaattt gggaaagagc aaaataatca ggggctttcc ctccagtgat tcctaaccag
     1621 tagtaggccc aaggttcttt actatctgct tgataaaagg ttagttcctc tggttttaat
     1681 agaaagaaat ctccttcttt taaatcaaca attttaccct tgtaatgaaa ttttccttgt
     1741 cctttagtaa tgtaatgtag gacgtatgta tcacgaatgg ctggaccaaa agagtaatta
     1801 ggtgtgcatt cctcatatcc ataaaagctt agggcaaggt cgattgttcc agtctggtat
     1861 tctgaaaaaa ctaacatgtg ctacctccta cctaacattt taccatattc ttgagacatt
     1921 tttctatttt ggaaagcgat ttcaggtgat aaaatagagt caataaagtg atgaggtgaa
     1981 gtaaatggga gttaggatag agaataatct attttatgtt gagagtaaaa atctaagttt
     2041 gattattgaa aatcgaaatg gctacttact tttgaaacat ttaggaaaga ctattaagaa
     2101 ctataaaggt tccaatagtg tttatgaacg agaccatgcc ttttcaggaa atccaacagc
     2161 tactaatcga acctttagtt tagatactca acgtcaaatt tttggacaac atggcttagg
     2221 agattttagg aaaccaacca tacaggttca gcataatgta actgaagtaa cagactttcg
     2281 atttgtagaa gcaaagattt taaaaggtca gaatggtcca cagggcttac cttctccaca
     2341 tagcatggac gatacagaga ctcttgtctt aatgttagaa gattctaagg ctcaacttag
     2401 tctgactttg tattatacta cttttaataa tgatgcgact attgctagct acagtaaatt
     2461 agataataat agtaatcagg aagttgtcat ccataaagat ttttctttta tggctgattt
     2521 tccagctgca gattacgaaa tagtaactct gcagggtgct tatgctcgtg aaaagactgt
     2581 tagacgtcaa caggtagaac aaggaatctt ttcgattagt tcaaaccgag gtgcttctgg
     2641 tcatgctcaa acaccagctc ttctactatg cgaacaagga gtcacagagg atgctgggaa
     2701 tgtgtttgct attcaactaa tgtatagtgg caactttgaa gcttttgttc aaaaaaatca
     2761 attgaatgaa gttcgggtgg ctattggcat taatccagaa aacttttctt ggaagttagc
     2821 ccctgaggaa tactttgaaa caccggtagc tttagtgact cattcagatc agggattaac
     2881 tggtattagt catgaaagtc agaattttgt actgaagcac attatgctaa gtgaattttc
     2941 taaaaaagaa cgtccaattc taatcaataa ctgggaagct acttactttg actttcagag
     3001 agaaaaactg ttagagttag cagatgaagc taagaaagtt ggcattgaac tttttgtatt
     3061 agatgatggt tggtttggca atcgttttga tgataatcgt gctttaggtg attgggttgt
     3121 taatgaggaa aaactgggtg gaagtctaga aagtctgatt tcagctatcc atgaaagagg
     3181 tttgcagttt ggactttggt tagaacccga aatgatttct gtagatagtg atttgtatcg
     3241 tcaacatcct gactgggcta ttcaggttcc tggctatgag catacttatt ctcggaatca
     3301 attagtactt aatcttgcca atcctcaggt agtagaatac ttgaaaagtg tcttagatca
     3361 actcctatct tatcatgaga ttgattacat taaatgggat atgaaccgca atatcactaa
     3421 gctagggaat ggattaactt atctagagac acagatgcaa tctcatcagt acatgctggg
     3481 gctttatgaa ctcgtttctt atctgacaga gaagcacagc catattctct ttgagtcctg
     3541 ctctggtggt ggtggacgaa atgatcttgg tatgatacgc tatttcccac aagtctgggc
     3601 tagtgataat actgatgcca ttgcacgttt accaattcaa tacggttcat cctatttcta
     3661 tccaaccatt tctatggggg ctcatgtgtc agcagtaccg aatcatcaga tgggacgaat
     3721 gacaccatta gaaacacgtg gccatgtagc aatgatggga aatttgggct atgagcttga
     3781 tttgacaaat ttatcagatg aagagaaagc tacgattgct aatcaggtga acttgtataa
     3841 agaattacga ccagtagttc agttaggaca gcagtataga ctaattaatc ctgatactgc
     3901 atccaatgaa gcagctgtac aatttaatta cggaaatcaa acgattgtaa cctacgttcg
     3961 cgttttatct gttgtagaga ccatggaaac aactttaaaa ttaaaagatt tggatgaaga
     4021 gggactatat aaattacagg aaaatggcga agtttactca ggtgcagaac taatgtatgc
     4081 gggcttaact gttatcttat cccaaggaga ttttttgagt agacagtata tttttagaaa
     4141 actatgatat gtactgaccc caaaagttag atttattctg tctaactttt ggggtgcagt
     4201 tcaataaggg tgtataaatt tataaaggag gctttctaat ggaatggtat aaaaaaatcg
     4261 gacttcttgc aactacaggt ttagctttgt ttgggctcgg cgcttgctcc aactatggta
     4321 aatctgcgga tggcacagtg accatcgagt atttcaacca gaaaaaagaa atgaccaaaa
     4381 ccttggaaga aatcactcgt gattttgaga aggaaaaccc taagatcaag gtcaaagtcg
     4441 tcaatgtacc aaatgctggt gaagtattga agacacgcgt tctcgcagga gatgtgcctg
     4501 atgtggtcaa tatttaccca cagtccattg aactgcaaga atgggcaaaa gcaggtgttt
     4561 ttgaagattt gagcaacaaa gactacctga aacgcgtgaa aaatggctac gctgaaaaat
     4621 atgctgtaaa cgaaaaagtt tacaacgttc cttttacagc taatgcttat ggaatttact
     4681 acaacaaaga taaattcgaa gaactgggct tgaaggttcc tgaaacctgg gatgaatttg
     4741 aacagttagt caaagatatc gttgctaaag gacaaacacc atttggaatt gcaggtgcag
     4801 atgcttggac actcaatggt tacaatcaat tagcctttgc gacagcaaca ggtggaggaa
     4861 aagaagcaaa tcaatacctt cgttattctc aaccaaatgc cattaaattg tcggatccga
     4921 ttatgaaaga tgatatcaag gtcatggaca tccttcgcat caatggctct aagcaaaaga
     4981 actgggaagg tgctggctat accgatgtta tcggagcctt cgcacgtggg gatgtcctca
     5041 tgacaccaaa tgggtcttgg gcgatcacag cgattaatga acaaaaaccg aactttaaga
     5101 ttgggacctt catgattcca ggaaaagaaa aaggacaaag cttaaccgtt ggtgcgggag
     5161 acttggcatg gtctatctca gccaccacca aacatccaaa agaagccaat gcctttgtgg
     5221 aatatatgac ccgtccagaa gtcatgcaaa aatactacga tgtggacgga tctccaacag
     5281 cgatcgaagg ggtcaaacaa gcaggagaag attcaccgct tgctggtatg accgaatatg
     5341 cctttacgga tcgtcacttg gtctggttgc aacaatactg gaccagtgaa gcagacttcc
     5401 ataccttgac catgaactat gtcttgaccg gtgataaaca aggcatggtc aatgatttga
     5461 atgccttctt taacccgatg aaagcggatg tggattagga gtagagagac tatgaaaaaa
     5521 gtattacaaa aatattgggc atgggctttt gtggtcatcc ccctcttgtt acaagcaatt
     5581 ttcttctatg tgccgatgtt tcaaggagcc ttttacagtt ttaccaactg gacaggattg
     5641 acttacaact acaaatttgt cggcttaaac aactttaagc tcctcttcat ggatccaaaa
     5701 ttcatgaatg cgattggctt taccgcaatc attacgattg ccatggtggt tggtgagatt
     5761 gcactcggga tcttcattgc gcgtgtcttg aactctaaaa tcaaaggcca aaccttcttc
     5821 cgtgcttggt tcttcttccc agctgtttta tctggtttga cagtggcttt gatcttcaag
     5881 caagtcttca actacggtct tccagcgatt gggaatgccc ttcatattga atttctccaa
     5941 accagtcttt tagggactag gtggggagca atctttgcgg ctgtctttgt ccttctttgg
     6001 caaggggtgg ctatgcccat catcatcttc ctagctggtt tgcaatctat tccaactgag
     6061 attacagagg cagcaaggat tgatggtgcg actagcaagc aagttttctg gaacattgaa
     6121 ttgccttact tgctaccaag tgtctctata gtctttatcc tagccctaaa aggtgggctg
     6181 actgcctttg accaagtctt tgccatgacc ggtggtggtc caaacaatgc cacaacctca
     6241 cttgggctct tggtttataa ctatgccttt aaaaacaacc aattcggtta tgccaatgcc
     6301 attgccgtaa tcttgttctt cttaattgta gtgatttcga tcatccaatt gagagtatct
     6361 aagaaatttg aaatttaaga ggagaagcat gatgaaacaa gatgaaagaa aagccctgat
     6421 tggcaaatac attctattga ttctaggatc ggttctgatt ttagtgccgc tccttgctac
     6481 cctctttagt tccttcaaac tcactaagga tattgtagat aatttctttg gctttccaac
     6541 caacttcaca tgggacaact ttagccgtct cttagctgat gggattggag gctattattg
     6601 gaactctgtc gtcatcactg tcttgtcttt acttgcagta atgatcttta tccctatggc
     6661 agcctactcc atcgctcgca atatgagtaa aagaaaagcc tttaccatca tgtataccct
     6721 cttaatcctc ggaatcttcg tacctttcca agtcatcatg attccgatta cggttatgat
     6781 gagtaaactc ggtttggcta atacctttgg tttgatcttg ctctacttga cctatgcgat
     6841 tccacagacc ctctttctct atgttggcta tatcaaaatc tcgattccag aaagtctgga
     6901 tgaagcagca gagatcgatg gggctaatca atttacaacc tatttccgca tcatcttccc
     6961 aatgatgaaa ccgatgcatg cgacaaccat gatcatcaat gccctttggt tctggaatga
     7021 cttcatgttg ccactccttg tcttgaaccg ggattccaaa atgtggactc tgcctttgtt
     7081 ccaatacaac tacgcaggcc aatatttcaa cgactacgga ccaagctttg cctcttacgt
     7141 ggtcggcatt atcagtatca ccattgtcta tctcttcttc caacgccata tcatttcagg
     7201 aatgagcaac ggggcagtga agtaatactc aatgaaaatc aaagagcaaa ctaggaagct
     7261 agccgcaggt tgctcaaaac actgttttga ggttgtggat gaaactgacg aagtcagtaa
     7321 ccatacctac ggtaaggtga agctgacgtg gtttgaagag attttcgaat agtataagaa
     7381 gagctcatgg aatctatagt ctttgacaca gaggaagagt tcagcttgtt gataattcta
     7441 tgtagaaaac aagaaagtag tactttgttt atcacagtct atattagtac gagtttgatg
     7501 ataaatagag atttaaaatg aattttattt atgaagccag atcatatgag acttggcttc
     7561 aattagaaaa aggagagtaa tgatgccaat tcaaaataaa accatgttga ttacctattc
     7621 tgatagcctt ggaaataatc ttaaagactt atatgataat ttggaagagc attttggaga
     7681 tgctattgga ggagttcacc ttttaccatt tttcccatca acaggtgatc gtggatttgc
     7741 gccagttgac tacgacgaag tggactcagc ttttggtgat tgggaggatg ttaagcgttt
     7801 aggtgagaga tattatctta tgtttgattt tatgattaat catatttctc gtcaatccaa
     7861 gtattataag gactatcaag aaaaacatga agccagtgaa tttaaagatc tctttttaaa
     7921 ctgggataag ttttggccag aaaaccgtcc gacacagtct gatgtagatt taatttacaa
     7981 gcgtaaggat cgtgcaccaa agcaagagat tgtgtttgaa gatggttcag tggaacattt
     8041 gtggaatacc tttggtgagg agcagattga tcttgatgtg accaaagaag taactatgga
     8101 atttatccgt aagaccattc agcacttggc aagtaatggg tgtgatttga ttcgtctaga
     8161 tgcctttgct tatgcagtga agaaattgga tactaatgat ttctttgtgg aaccagatat
     8221 ttgggattta ttggacaaag ttcgagatat cgctgctgag tatgggacag agcttttacc
     8281 tgagattcat gaacactatt cgattcagtt taaaatagca gaccatgatt actatgttta
     8341 tgattttgct cttccaatgg tgacacttta tactctttac agttccagaa cagagcgttt
     8401 ggctaagtgg ttaaagatga gcccgatgaa gcaatttacg acgctagata cccatgatgg
     8461 gattggagta gtagatgtca aggatatcct gaccgatgag gagattgact atgcttcaaa
     8521 tgaactctat aaggttggag ccaatgtcaa acgtaagtac tctagtgccg agtataacaa
     8581 cttagatatc taccaaatca attcaaccta ctattcagcg cttggagatg atgatgtcaa
     8641 gtattttctc gctcgtctaa ttcaagcttt tgccccaggt attcctcagg tttactatgt
     8701 gggtctatta gcaggcaaga atgacttgaa attattagaa gaaactaaag aaggtcgaaa
     8761 tattaatcgt cattactata gcaacgagga aatagcaaaa gaagtgcaac gatctgttgt
     8821 gaaggccctt ctcaatctat tttctttccg taaccgttca gaagcctttg atctagaagg
     8881 gactattgag atagagacac caacagttca cagcattgta atcaaacgtc aaaataaaga
     8941 taagtccgta acagcagtag cagaaattga tttgcaaaat cagacttatc gggtaattga
     9001 gaatggagtt gaagtaacat tttgaagcct tgatatggtg gataaaatag ggtttttatt
     9061 ttggaaaacg tttcctttgt tttcaaattg ctaaaaaagt ggtacaatat aggatagctt
     9121 actattatct gaatcagctg atttggagag aaaggattca ttttgaaatc aataggcttt
     9181 attgaaaagc tgaaggggtt gtctagtaaa gagctgattt tattgggaat tatcctaagt
     9241 atctttttac ccttttatct ttttgtagtt gtactctgtt tatatattat cagtttgatt
     9301 tttacaggag acatgaaaag tattcttcag aaaatggggg agcatccgat gctgcttctt
     9361 tttcttagct atagtactgt tatatccatt cttgcacaaa attggatggg tcttgtggct
     9421 tcagtaggaa tgtttctatt tactattttc tttttgcact atcagtcgat tttatcccat
     9481 aaattctttc gattgatttt gcagttcgtc ttgtttggta gtgtcttgtc agctgctttt
     9541 gccagtttag aacatttcca aattgtgaag aaatttaact atgcttttct ttcacccaat
     9601 atgcaggtgt ggcatcagaa ccgggcagaa gtgactttct ttaatcctaa ttattatgga
     9661 attatttgtt gtttctgtat tatgattgct ttctatctgt ttacaacgac caagttgaat
     9721 tggttgaaag tattctgtgt gattgcaggc tttgttaatc tctttggttt gaactttact
     9781 caaaatcgaa ctgcctttcc tgctattatc gctggagcaa ttatctatct ctttacgact
     9841 attaaaaact ggaaggcctt ttggcttagt attggggtct tcgcgattgg tttgagtttc
     9901 ctcttttcta gtgatttggg agttcgaatg ggtactttag actcttctat ggaagaacgc
     9961 atttctatct gggatgctgg gatggccttg tttaagcaaa atcctttttg gggtgaaggg
    10021 ccattgacct atatgcactc ttatcctcgg atacatgctc cttatcatga acatgcccac
    10081 agtctttata ttgatacgat tctgagttac ggaattgtgg gtaccatttt attagttttg
    10141 tcttctgttg ctcctgttcg cttgatgatg gatatgagtc aggagtcggg gaaacgtccg
    10201 attatcggcc tttatctatc tttccttaca gtggttgctg tgcacggaat ttttgacttg
    10261 gctctcttct ggattcagtc aggctttatt ttcttgctag ttatgtgcag tattccattg
    10321 gagcatcgaa tgttggtatc ggacatgacg gattaagttt tataagtttg aaatcttcta
    10381 ccttgggtag gagatttttt tatacgatgg gcatgtcgtg catctgaggt gtaagtcctc
    10441 tgtagggatc cactaccggt gaatacaata gcgctcccca agcctgacta tcgtgaggta
    10501 gcggattaaa atggtctggg gatagatcgt tttaagtctg acgcaggaaa taagaattgt
    10561 cagagaaagc aatagtgaaa tcgtaggcta tgaatagaaa cgtgatagcg aggagcatat
    10621 agtggataag gtagcgagaa attttaaagt ataaaagata gtcttaatct atatatgtaa
    10681 atcacaaaag taattgaatt cggaataagg ttggtgtgaa aagacatagt aaactgaatc
    10741 ttgaatacta gaatgatact tctacaatat tgttataatt taatttgaat tccaaaatca
    10801 cattgttccg tccgtatttc attttactgt atgtcttcct aaactccaaa gtttctgcgt
    10861 caaatttcct attttcactg tagtctttga cacttcgaac gacaatttga atcatatatt
    10921 caaaaaaaaa tgaaaaattt gtgccaacaa gcctaaatat tctgaaaatt tgtttgattt
    10981 ttaatggata atgtgatata atggttgtca atctataatt cacataaaca gaggagtaga
    11041 aaatgaaaaa aaatcgtgta tttgctacag caggtcttgt tttattagca gcaggtgtac
    11101 ttgcagcatg cagttcttca aaatcatctg attcatcagc ccctaaagct tatggctatg
    11161 tttatacagc agacccagaa accttggact acctgatttc aagtaaaaat agtacaacag
    11221 tagtgacttc aaatgggatt gatggtttat tcactaacga taattacggt aatcttgctc
    11281 ctgcagttgc agaggattgg gaagtctcta aggatggttt gacctacact tataagattc
    11341 gtaaaggggt taaatggttt acctctgatg gagaagaata tgcagaggtg acggctaaag
    11401 atttcgtgaa cggtttaaaa cacgcagcag ataaaaaatc agaagctatg tatttagctg
    11461 aaaattcggt taaaggcttg gcagattatc tatcaggaac ttcaacagat ttttcaacag
    11521 ttggtgtcaa ggcggttgat gattatacgt tacaatacac tttgaaccag cctgaaccgt
    11581 tctggaactc taagttgacc tattctattt tctggcctct gaatgaagaa ttcgaaacat
    11641 caaaaggaag cgattttgct aaaccaacag atccgacatc cttgctttat aatggtccat
    11701 tcttgttgaa agggttgact gcaaaatctt ctgtagagtt tgtaaaaaat gagcaatatt
    11761 gggataaaga aaatgtccac ctagatacta tcaatctagc ttactatgat ggatcagatc
    11821 aggagtcgct agagcgtaac ttcactagtg gagcttatag ttatgcccgt ctttacccta
    11881 ccagctccaa ctattctaag gttgcagaag aatacaagga caatatctat tacacacaat
    11941 caggctctgg gattgctggt ctgggtgtga atattgatcg ccaaagttac aactatactt
    12001 ctaaaactac agattcagag aaagtagcta ctaagaaggc attgcttaac aaagatttcc
    12061 gtcaagcctt gaattttgct cttgatcgct cagcttactc agctcaaatc aatggtaaag
    12121 atggagcagc tttagcagtt cgtaatttat ttgtaaaacc agactttgtt tcagctggtg
    12181 agaagacctt tggtgattta gtcgctgctc aacttcctgc ttatggtgat gagtggaaag
    12241 gtgtgaattt agctgatggg caggatggtt tattcaatgc tgacaaggcc aaggcagagt
    12301 ttgcgaaagc taagaaagct ttagaagcag acggcgttca gtttcctatt catctggacg
    12361 ttccagtaga ccaagcatca aaaaactaca tatctcgtat tcagtccttt aaacaatctg
    12421 tagaaacagt tcttggtgtt gaaaatgtcg ttgttgatat tcaacaaatg acaagtgatg
    12481 aattccttaa tattacttac tatgctgcca atgcttcatc tgaggattgg gatgtatcag
    12541 gaggagtttc atgggggcca gactatcaag acccatctac ttacctggat attttaaaaa
    12601 caactagcag tgaaactaca aaaacatatt taggatttga taatccaaat agcccttcag
    12661 tagttcaagt tggtttgaaa gaatacgata aattagttga tgaagctgcc aaagagacaa
    12721 gcgacttgaa tgtccgttat gaaaaatatg cagcggctca agcatggttg acagatagtt
    12781 cactctttat tcctgctatg gcttcttctg gtgcagcacc agtgctttca cgaattgttc
    12841 catttactgg agcttctgcg caaacaggct ctaaggggtc agatgtttac ttcaaatatt
    12901 tgaaattaca agataaagtg gtgactaagg aagagtatga aaaagctcgt gaaaaatggt
    12961 tgaaagaaaa agctgaatca aatgagaaag ctcaaaaaga attggcaagt catgtgaagt
    13021 aaataatttc taatagaact ttctctccat gaggagaagg ttcttttggg atttttaaag
    13081 gaaataatat gaaaaaatat atttttatgc gtgttttgcg gtcattggtt tcgattttct
    13141 tagtaacgac tttgacctac acgattatct ataccttggt tcctcgaaaa ttgattttca
    13201 agcaggatcc taactataat aaaattgcga caacggctga taaacgtgat aactatgaaa
    13261 atactgtgtt tgagcgtatg ggctacattg agtattacga tactaaagag ttgcaagaaa
    13321 aggcaagtag catggattct tctgtaacag tagaagcaaa tgcgaccaat aaagctattt
    13381 atgaaaagta catcaatcaa ttaggtcatg gttggacttt gggagaattt actgaaagtg
    13441 gtcaattcta tgctactcgt gaaattccaa tttttgaacg tgtttttcac ttctatgcta
    13501 acttgattga cattgaccat acaaataaaa tccaagaccc tgaaaatcca gacttgaaac
    13561 gctaccttcg ttttgaaaat gatccagcta tcggatggtc attggtcggt tcaggaacta
    13621 aacataaata tctcttgtac tttaacagtc agttcccatt tgttcatcaa aactttgtga
    13681 acttgaattt aggtgactct tacccaacct atgctaatac accagttctt caggttatta
    13741 ctcaaggtca aggacaaacc aaaactgccc aagttcagtt cccaacaggt aagaaaacgt
    13801 cttctgtaaa tatttactca agaacctaca agtcacctag tcaggctgac tctcgtgaag
    13861 tagctagcta tgggaaagat gatccttata cagcgactga aagtaattac caatatccat
    13921 ctatgattgt cagctctgct attactggtt tgattggttt ggttcttgcc tatgctcttg
    13981 ccgtgccact tggttcagcc atggctcgtt tcaagaacac ttggattgat agcctctcaa
    14041 cagggacttt gaccttcttg cttgctcttc caacgattgc cttggtttac atcgttcgat
    14101 tgattggatc atctattgcc cttccagatt cattccctat cttgggagct ggagattggc
    14161 gttcttacgt tttaccagca gtcatccttg gtttattggg tgctcctgtt acagccattt
    14221 ggattcgtcg ttacatgatt gacttgcaat ctcaagactt tgttcgtttc gctcgtgcaa
    14281 aaggtttgtc tgaaaaagaa atttcaaaca aacacatctt taaaaatgcc atggttccgc
    14341 tggtttcagg aattcctggt gccattattg gggttatcgg tggtgcaacc cttactgaaa
    14401 cagtcttcgc cttcccaggt atgggtaaaa tgttgattga ctctgtaaaa gcatctaata
    14461 actctatggt cgttggtctt gtcttcatct ttacatgtat ttctatcttc tcacgtcttt
    14521 tgggagatat ttggatgact attattgacc cacgtattaa attgactgag aaaggaggca
    14581 aataatgtct acaatcgata aagaaaaatt tcagtttgta aaacgtgacg attttgcctc
    14641 tgaaactatt gatgcgccag catattctta ctggaaatca gtgtttaaac aatttatgaa
    14701 gaaaaaatca actgtagtca tgttgggaat cttggtagcc atcattttga taagtttcat
    14761 ctacccaatg ttttctaagt ttgatttcaa tgatgtcagc aaggtaaacg actttagtgt
    14821 tcgttatatc aagccaaatg cggagcattg gttcggtact gacagtaacg gtaaatcgct
    14881 ctttgacggt gtctggttcg gagctcgtaa ctccatcctc atttctgtga ttgcgacagt
    14941 gattaacttg gttatcggtg tttttgtcgg tggtatttgg ggtatttcaa aatcagttga
    15001 ccgtgtcatg atggaagttt acaacgtcat ctcaaacatc ccacctcttt tgattgttat
    15061 tgtcttgact tactcaatcg gagctggatt ctggaatctg atttttgcca tgagcgtaac
    15121 aacatggatt ggtattgcct tcatgattcg tgtgcaaatc ttgcgctatc gtgacttgga
    15181 atacaacttg gcgtcacgta ctttgggaac accaaccttg aagattgttg ccaaaaatat
    15241 catgcctcaa ttggtatctg ttattgtgac aaccatgact caaatgcttc caagctttat
    15301 ctcatacgaa gccttcttgt ctttcttcgg tcttggatta ccgattacag tgccaagttt
    15361 gggtcgtttg atttcggatt attcacaaaa cgtaacaacc aatgcttact tgttctggat
    15421 tccattgaca acccttgtct tggtatcctt gtcccttttc gtagttggtc aaaacttagc
    15481 ggatgctagt gatccacgta cacatagata ggagtagaaa tgacaaaaga aaaaaatgta
    15541 attttgactg ctcgcgatat tgtcgtggaa tttgacgttc gtgacaaagt attgacagcc
    15601 attcgcggcg tttcccttga actagtcgaa ggagaagtat tagccttggt aggtgagtca
    15661 ggatcaggta aatctgtttt gacaaagacc ttcacaggta tgctcgaaga aaatggtcgt
    15721 attgcccaag gtagtattga ctaccgtggt caggacttga cagctttatc ttctcacaag
    15781 gattgggaac aaattcgtgg tgctaagatt gcgactatct tccaggaccc aatgactagt
    15841 ttggacccca ttaaaacaat tggtagtcag attacagaag ttattgtaaa acaccaagga
    15901 aaaacagcta aagaagcgaa agaattggcc attgactaca tgaataaggt tggcattcca
    15961 gacgcagata gacgttttaa tgaataccca ttccaatatt ctggaggaat gcgtcaacgt
    16021 atcgttattg cgattgccct tgcctgccga cctgatgtct tgatctgtga tgagccaaca
    16081 actgccttgg atgtaactat tcaagctcag attattgatt tgctaaaatc tttacaaaac
    16141 gagtatcatt tcacaacaat ctttattacc cacgaccttg gtgtggtggc aagtattgcg
    16201 gataaggtag cggttatgta tgcaggagaa atcgttgagt atggaacggt tgaggaagtc
    16261 ttctatgacc ctcgccatcc atatacatgg agtctcttgt ctagcttgcc tcagcttgct
    16321 gatgataaag gggatcttta ctcaatccca ggaacacctc cgtcacttta tactgacctg
    16381 aaaggggatg cttttgcctt gcgttctgac tacgcaatgc agattgactt cgaacaaaaa
    16441 gctcctcaat tctcagtatc agagacacat tgggctaaaa cttggcttct tcatgaggat
    16501 gctccaaaag tagaaaaacc agctgtgatt gcaaatctcc atgataagat tcgtgaaaaa
    16561 atgggatttg cccatctggc tgactaggag gaaggaaatg tctgaaaaat tagtagaaat
    16621 caaagattta gaaatttcct tcggtgaagg aagtaagaag tttgtcgcgg ttaaaaatgc
    16681 taacttcttt atcaacaagg gagaaacttt ctcgcttgta ggtgagtccg gtagtgggaa
    16741 aacaactatt ggtcgtgcta tcatcggtct aaatgataca agtaatggag atatcatttt
    16801 tgatggtcaa aagattaatg gtaagaaatc gcgtgaacaa gctgcggaat tgattcgtcg
    16861 aatccagatg attttccaag accctgccgc aagtttgaat gaacgtgcga ctgttgatta
    16921 tattatttct gaaggtcttt acaatcaccg tttatttaag gatgaagaag aacgtaaaga
    16981 gaaagttcaa aatattatcc gtgaagtagg tcttcttgct gagcacttga ctcgttaccc
    17041 tcatgaattc tcaggcggtc aacgtcaacg tatcggtatt gcccgtgcct tggtcatgca
    17101 accagacttt gttattgcag atgagccaat ttcagccttg gacgtttctg tacgtgccca
    17161 agtcttgaac ttgctcaaaa aattccaaaa agagctcggc ctgacctatc tcttcatcgc
    17221 ccatgacttg tcggttgttc gctttatttc agatcgtatc gcagttattt acaagggtgt
    17281 tattgtagag gttgcagaaa cagaagaatt gtttaacaat ccaattcacc catatactca
    17341 agccttgctt tcagcggtac caatcccaga tccaatcttg gaacgtaaga aggtcttgaa
    17401 ggtttacgac ccaagtcaac acgactatga gactgataag ccgtctatgg tagaaatccg
    17461 tccaggtcac tatgtttggg cgaaccaaac cgaattggca cgttatcaaa aaggactaaa
    17521 ctagtaatgg ttttataatt tccatgtcaa tttttatgga aattaggctc tttgtcaact
    17581 gtagtgggtt gaagtcagct aagctcgaga aaggacaaat ttcgtccttt cttttttgat
    17641 gttcaaagcg ataaaaatcc gttttttgaa gttttcaaag tttcgaaaac caaaagcatt
    17701 gcgcttgata agtttgatga gattattggt cgcttccagt ttggcattag aatagtgtag
    17761 ttgaagggcg ttgataatct tttctttatc tttgaggaag gttttaaaga cagtctgaaa
    17821 aataggatga acctgcttaa gattgtcctc gataagttcg aaaaatttct ctggttcctt
    17881 attctggaag tgaaaaagca agagctgata gagctgatag tggtgtttca agtcttccga
    17941 ataactcaaa agcttgttta aaatctcttt attggttaag tgcatacgaa aagtaggacg
    18001 ataaaattgc ttatcactca gtttacggct atcctgttgt atgagcttcc agtagtgctt
    18061 gatagccttg t
//
LOCUS       DAVKQB010000045        17022 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKQB010000045 DAVKQB010000000
VERSION     DAVKQB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17022
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.045"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            314..1360
                     /gene="pheS"
                     /locus_tag="VW528_001603"
     CDS             314..1360
                     /gene="pheS"
                     /locus_tag="VW528_001603"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9912405.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1360..1869
                     /locus_tag="VW528_001604"
     CDS             1360..1869
                     /locus_tag="VW528_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9912406.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1946..4351
                     /locus_tag="VW528_001605"
     CDS             1946..4351
                     /locus_tag="VW528_001605"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9912407.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNESAADALSVSIGTDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLAETDLVITVADKPVALAGVMGGQATEISEKS
                     SRVILEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4419..5420)
                     /locus_tag="VW528_001606"
     CDS             complement(4419..5420)
                     /locus_tag="VW528_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9912408.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTDSGIAPVTVIVKKSYDFYTKAETFHTTRFGTIVLHSRKQDIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5576..6065
                     /locus_tag="VW528_001607"
                     /pseudo
     CDS             5576..6065
                     /locus_tag="VW528_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6149..6415
                     /locus_tag="VW528_001608"
     CDS             6149..6415
                     /locus_tag="VW528_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9912409.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            6676..8925
                     /gene="metE"
                     /locus_tag="VW528_001609"
     CDS             6676..8925
                     /gene="metE"
                     /locus_tag="VW528_001609"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9912410.1"
                     /translation="MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLSAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFTTERVGENAELRARIAG
                     LTDADYTRLPVFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            8989..9855
                     /gene="metF"
                     /locus_tag="VW528_001610"
     CDS             8989..9855
                     /gene="metF"
                     /locus_tag="VW528_001610"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9912411.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELVPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKERAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNAYTAKYIHQ
                     ATHALFNHQSLG"
     gene            10540..12753
                     /gene="pnp"
                     /locus_tag="VW528_001611"
     CDS             10540..12753
                     /gene="pnp"
                     /locus_tag="VW528_001611"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9912412.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12769..13386
                     /gene="cysE"
                     /locus_tag="VW528_001612"
     CDS             12769..13386
                     /gene="cysE"
                     /locus_tag="VW528_001612"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9912413.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13398..14282
                     /locus_tag="VW528_001613"
     CDS             13398..14282
                     /locus_tag="VW528_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9912414.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14369..15712
                     /locus_tag="VW528_001614"
     CDS             14369..15712
                     /locus_tag="VW528_001614"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9912415.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15705..16091
                     /locus_tag="VW528_001615"
     CDS             15705..16091
                     /locus_tag="VW528_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9912416.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16095..16979
                     /locus_tag="VW528_001616"
     CDS             16095..16979
                     /locus_tag="VW528_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9912417.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
BASE COUNT         5052 a         3329 c         3895 g         4746 t
ORIGIN      
        1 gctataaagt tagataagaa taagctaaca agcagaagta agataggtct tgcaagaatg
       61 gtaaaaatct gataaactaa gtagtaattg aataggaatc tgaaagacta gtagcccaag
      121 ggaagtatat cagggaggag agccgtgact gcaagctctc tatatgaaag ctgggtgaat
      181 tcacttgcgc atggatttag aaatgaaggt ctgacttgtc agatgaaaac ggatggtacc
      241 gcgtgtcaac gctccgagtg gagtttttgg catgtggttt tctttttatc tacgagagac
      301 tgatggagga aatatgtcaa ctattgaaga acaattaaaa gcgcttcgcg aagaaacgct
      361 ggctagcttg aagcagatta ctgctggaaa tgaaaaagag atgcaagatt tgcgtgtctc
      421 tgtccttggt aaaaagggtt cgctcactga aatcctcaaa gggatgaaag atgtttctgc
      481 tgagatgcgt ccaatcatcg ggaaacacgt caatgaagct cgtgatgtct tgactgctgc
      541 ttttgaagaa acagctaagc tcttggaaga aaagaaagtc gcggctcaac tggctagcga
      601 gagtatcgat gtgacgcttc caggtcgtcc agttgcgact ggtcaccgtc acgttttgac
      661 acaaaccagt gaagaaatcg aagatatctt catcggtatg ggttatcaag tcgtggatgg
      721 ttttgaagtg gagcaagact actataactt tgaacgtatg aaccttccaa aagaccaccc
      781 agctcgtgat atgcaggata ctttctatat cactgaagaa atcttgctcc gtacccacac
      841 gtctccagtt caggcgcgtg ctatggatgc ccatgatttt tctaaaggtc ctttgaagat
      901 gatctcgcca gggcgtgtct tccgtcgcga tacggacgat gcgacccaca gtcaccaatt
      961 ccaccaaatc gaaggcttgg tagttgggaa aaatatctct atggctgatc ttcaaggaac
     1021 gcttcagttg attgtccaaa aaatgtttgg tgaagagcgt caaattcgtt tgcgtccatc
     1081 ttacttccca ttcacagagc catctgttga ggtggatgtt tcttgcttca agtgtggtgg
     1141 agaaggctgt aatgtatgta agaaaacagg ttggatcgaa attatggggg ccggtatggt
     1201 tcacccacgt gtccttgaaa tgagtggtat cgatgcgact gtatactctg gctttgcctt
     1261 tggtcttgga caagagcgtg tagctatgct ccgttatgga atcaacgata tccgtggatt
     1321 ctaccaagga gatgtccgct tctcagaaca gtttaaataa tgattagaaa agtagaaatg
     1381 gcagatgttg aggtgttggc taaaattgcc aaacaagcct ttcgtgaaac ctttgcgtat
     1441 gataatacgg aagagcagtt acaggaatac tttgaagagg cttatagtct gaaaactttg
     1501 tcaactgagt tgggaaatcc tgactctgaa acctatttca ttatgcatga ggaggagata
     1561 gctggttttc tcaaagtcaa ctggggaagt gctcaaactg agagagaatt agaggacgct
     1621 tttgaaattc aacgcctcta tgtgctacaa aaattccaag gatttggact aggtaagcaa
     1681 ctgtttgaat tcgcacttga acttgctaca aaaaatagtt tttcttgggc ttggttaggt
     1741 gtttgggagc ataatacaaa agctcaagcg ttttataatc gatatggttt tgaaaaattt
     1801 agccaacatc attttatggt tggtcaaaaa gtagatacgg attggttact gagaaagaaa
     1861 ttaaggtaag aagaggattc ttctattgaa atgataggaa aggaaaagaa ctagagtgac
     1921 aactgtcatt ctggagaact aaattatgct tgtatcttat aaatggttaa aagaattggt
     1981 ggacattgat gtgccatcac aagagttggc tgaaaaaatg tcaactacag gaatcgaggt
     2041 agagggtgtc gaatcaccag ctgctggtct ctcaaaaatt gtcgtcggtg aggtcttgtc
     2101 ttgcgaagat gtgccagaga ctcacctcca tgtttgtcag gttaacgttg gcgaagaaga
     2161 agagcgtcag atcgtttgtg gtgccccaaa tgtgcgtgct gggatcaagg tcatggtggc
     2221 tcttccagga gctcgtatcg ctgataacta caaaatcaaa aaaggaaaaa tccgtggttt
     2281 ggagtcactt ggaatgatct gttcacttgg tgaattggga atttctgact cagttgtgcc
     2341 taaggaattc gcagatggca tccaaatctt gccagaaaat gccgtgccag gtgaggaagt
     2401 cttttcttac ctagacttgg atgatgaaat catcgaactt tccatcacac caaaccgtgc
     2461 agatgccctt tctatgtgtg gagtggctca cgaagtggca gccatctatg acaaggcagt
     2521 caactttaaa gaatttactc taacagaaac taatgaatct gcggcagatg ccctttctgt
     2581 cagcattggg acagacaagg cgccttacta tgcagctcgt atcttggaca atgtgactat
     2641 cgcaccaagt ccacaatggt tgcaaaacct tctcatgaac gaaggcatcc gtcccatcaa
     2701 taacgttgta gacgtgacaa actacatcct gctctacttt ggtcaaccta tgcatgcttt
     2761 tgacttggac acatttgaag ggactgacat ccgtgtgcgt gaagcgcgtg ctggtgaaaa
     2821 gttggtgacc ttggacggtg aagaacgaga cttggctgag acagacctcg tgattacagt
     2881 tgctgacaaa ccagtagccc ttgccggtgt tatgggtggt caggctacag aaatttctga
     2941 aaaatctagt cgtgttatcc ttgaagctgc tgtttttaat ggcaaatcta tccgtaagac
     3001 aagtggtcgc ctgaaccttc gttctgagtc atcttctcgc tttgaaaaag gaattaatgt
     3061 ggcaacagtt aatgaagccc ttgatgcggc agctagcctg attgcggaac ttgcaggtgc
     3121 gacggtgcgt aagggtatcg tttcagcggg tgagcttgat acttcagatg tagaagtttc
     3181 ttcaactctt gctgatgtta accgtgtcct cggaactgag ctgtcttatg ctgatgtcga
     3241 agacgtcttc cgtcgtcttg gctttggtct ttctggaaat gcagacagct ttacagtcag
     3301 cgtaccacgt cgtcgttggg atatcacaat cgaagctgat ctctttgaag aaatcgctcg
     3361 tatctatgga tatgaccgtt tgccaaccag ccttccaaaa gacgatggta cagctggtga
     3421 attgactgtg acacaaaaac tccgccgtca agttcgtacc attgctgaag gagcaggttt
     3481 gacagaaatc atcacctata ctctaacaac tcctgaaaaa gcagttgagt ttacggctca
     3541 accaagtaac cttacggaac tcatgtggcc aatgactgtg gatcgttcag tcctccgtca
     3601 aaatatgatt tcaggtatcc ttgataccgt tgcctacaac gtggctcgta agaataaaaa
     3661 cttggccctt tacgagattg gaaaagtctt tgaacaaaca ggtaatccaa aagaagaact
     3721 tccaaatgaa atcaacagtt ttgcctttgc cttgacaggc ttggttgctg aaaaagattt
     3781 ccaaacagca gcagttccag ttgatttctt ctatgctaag ggaatccttg aagcgctctt
     3841 tactcgcttg ggactagaag tgacctatac agcaacagct gagattgcca gccttcaccc
     3901 aggacgtaca gcagtcattt cactcggtga ccaagttctt ggttttcttg gccaagtgca
     3961 tccagtcact gccaaggctt acgatattcc agaaacgtat gtagctgagc ttaacctttc
     4021 agccatcgaa ggggcgctcc aacctgctgt tccatttgtg gaaatcacga aattcccagc
     4081 agtcagccgt gacgttgccc ttctcctcaa ggcagaagta actcaccaag aagttgtaga
     4141 tgctatccaa gctgccggcg tgaaacgttt gacagatatc aaactctttg acgtcttctc
     4201 aggtgagaaa ttgggacttg gtatgaagtc catggcttat agcttgacct tccaaaatcc
     4261 agaagatagc ttaacggacg aagaagtcgc acgctatatg gaaaaaatcc aagcatcgct
     4321 cgaagaaaaa gtgaatgcag aagtgcgtta aacgatagta ggatataaat tccccctcta
     4381 gtttatgaac actagagggg tgattttata taaaataatt aaaatgtgat ttctgtaaaa
     4441 atacatctat gatcagagtt ttgaaaactt acaatgtcta aatctttaac atagtagtgg
     4501 tttttaggca ataaaatatg atctatcgtt gcattaaaaa gttttggact ttggctattc
     4561 caagttcctc tttcaaaagg tggcagtgca tttaatgcgt ccctatgaga gcttattttt
     4621 gcaagtgctc catgacgcat agttgcatta aaatcacctg caataatagc ctttggatat
     4681 tttgaagcca attgattatg aatgatgttt aagtcttgct tccagatttc cattaaacct
     4741 ggcagaggag gagcagtatg caaagcaatg atatctggta tatcttgttt tctcgaatgt
     4801 aatacaattg tcccgaaccg tgttgtatga aaagtttcag cttttgtata gaaatcgtaa
     4861 cttttcttga caatcacagt cacaggagct attccactgt cagtaggtgg agaagtaaaa
     4921 atatcatagt tggccataga aagtccaact tgatgaaaca atagtttgat tctctggttt
     4981 tcttgctcac ctctgatatt ggtagctagt tcagggaata tagccatatc ggcgtcaaaa
     5041 tggctaaaaa ttcgctcaat atgttgtgct tctatttgat tagcgacgtt ccaagttact
     5101 aattttaagg ttttagtctt atttgatgcg gaagaaaggg ttttatctgt tccgaataat
     5161 agtaatatga gcgataataa gagcaaaatg ccagagtaaa accgtatttg tttatttttc
     5221 ttgtaaaaag atagggaaaa aaatacgaaa cttaaaatgg cagccatagt tgcagtagct
     5281 aaggggaagg cataaatctg tgccaagata ggtctggtag atagccaaat attaagatta
     5341 gaagatggaa aaaaagaata agttatgata gggataacaa agagaatgac cgaaagggca
     5401 gagagaaatg ttttaatcat tgtgtgagta cctcaatttg caatggttgc tcggagctaa
     5461 tgtgactttg tgactttact tgattgatat tctttttctt gtcttcctta tagattaagt
     5521 acaacccaag taaaaaaagt ccaatttaag attgaaatga ttaaaaggca cagagatgca
     5581 agacaattat actacaaaag gtaaacattt gacaatcgat agccgtcgct taatcgaaag
     5641 atggaaaaaa gaaggaaaat caaatagaga agttgcctct ctacttggaa aagctcctca
     5701 aactatccac actgaaatca agcgtgggac agtccgaaaa tgtcttggaa aagggcgctt
     5761 caaagaggtt tattctgccg actacgctca acagtcttat gaaaacaatc gcaagcgctc
     5821 ggtcaagaaa tcaagcttga ccaaggaact aaaggaaaag attctccact atacataacc
     5881 aaaaattttt gcctgaaatg atggtgatgg ctaaaggggt taacgtggga atttcaacca
     5941 tttactattg gattcatcat ggaaagttgg ggttaagcaa acaggatttg ctttatccta
     6001 gaaaaggaaa atctgttaag aaacaagtta gtcccaattt taaacccgct gatcaatcta
     6061 tcgaatcata attcgctcat ttttttaaaa atacatagac gtaaaaaaaa gagggttaag
     6121 tgatgaagaa tagtgctatt gggagtaatt ggaaggatgt ccatctgagc tctttagcaa
     6181 ggaggaaatc cttgaaagtg atatgcgagt ggctatcatg agcgagttga ttgaggctag
     6241 gtatgaacaa ggaatcagtc agaaaaagct ggaagaagtc agtggaataa gccagcctgt
     6301 catagctagg atggagacag gaaagactag tcctcagttg gatacagtct taaaagtcct
     6361 agccagttta ggaaagacac tagcaatcgt cccacttgaa cagggaaaaa gttgactaga
     6421 atcagttata gtcttttcta ataacaagcc atagtcactt ataagaatta ctaataacgc
     6481 gtttgagcat tctaactaaa atacagctat aaaacaagct atacaaagga tttgagacgt
     6541 ttgcttcaag tctttttctt ttgtccgaaa cagatatata gtttcaaact atatcaaacc
     6601 ctaatttttt acaattatac agacgctttc ttttctgtta agatagtttc aacaacaaat
     6661 tttggaggac acatcatgtc aactacaatc gtcggtttcc ctcgtttggg cgaattccgt
     6721 gaattaaaat ttacaactga aaaatacttt agaaaagaaa tctcagagga agaactttta
     6781 tcagcagcaa aagacttgcg tgctaaacac tggaacattg tcaaagaaaa aggcatcact
     6841 gaaattccat caaatgactt ttctcactat gataacttcc tagatgcggc tttccttttc
     6901 aacgtggtgc ctgcatccgt tcaaaacttg gacttgtctg accttgagcg ctacttcgct
     6961 ttgggacgtg gttaccaggg agaaaaaggg gacgttcgcg cccttccaat gaagaaatgg
     7021 ttcaacacta actaccacta catcgttcct aaatttgaaa aagacactca agtaaaattg
     7081 gctggtcaca agattttcga tgagttccaa gaagccaaag aacttggatt gaacactcgt
     7141 cctgttcttg taggtccctt cactttcctt caattgtcag actttgaaga aggcgtgaaa
     7201 gcagacgact tcgtagatag cttagtggct gcttaccaag aagtttttgc taaattggct
     7261 gaacttggtg cgactcgcat ccaattggat gaagcggctc ttgtaaaaga cttgacagct
     7321 gaagaaaaag ctctcttctt gaacctttac aacaaactct tggctgacaa aaaaggtctt
     7381 gaagtcttgc ttcaaactta tttcggtgat gttcgtgacg tatacgctga ccttgtaaac
     7441 ttaccagtag atgctatcgg tcttgacttt gttgaaggta agaaaactct tgaactcgtt
     7501 aaaggtggct tcccagctga caagactctc tatgtaggta ttgtcaatgg taaaaacatc
     7561 tggcgcaaca actacgaaaa gagcttggct gttcttgagc aaatcccagc tgaaaacatc
     7621 gttttgacaa gctcttgctc acttcttcat gtgccattta caactgctaa tgaagaattt
     7681 gaaccagcac tcttgaacca ctttgccttt gcagttgaaa aattggatga aatccgtgac
     7741 ttggatgcta tccgcaatgg tcaaggttca gaagctcttg cagccaacaa agaactcttt
     7801 acgactgagc gtgttggtga aaatgctgaa cttcgtgcgc gtatcgctgg cttgacagac
     7861 gcagactaca ctcgtttgcc agtctttgca gaacgtgaag ctatccaaga agaagctttc
     7921 aaacttccag ctcttccaac aacaacgatt ggttcattcc ctcaaacaaa agaagttcgt
     7981 gccaaacgtt tggcttaccg taaaggtgaa ttgtctcaaa aagagtacga tgctttcctt
     8041 gctgaaacga ttgacgaatg gatcaagtgg caagaagata ttgactttga tgtccttgtt
     8101 cacggtgaat ttgagcgtaa tgacatggtt gagtacttcg gtcaaaactt gtcaggatac
     8161 ctcttctcta aaaatggttg ggtacagtca tacggtatgc gtggggtgaa accaccaatc
     8221 atctggggtg atgtcactcg tcttaaccct atcactgtta aatggtctag ctatgcacaa
     8281 agccgtacaa acaaacctgt taaaggtatg ttgactggac ctgttaccat cctcaactgg
     8341 tcattcccac gtgaagacat ctctatcaag gattcaactc ttcaaatcgc ccttgctatc
     8401 aaggatgaag tgcttgacct tgaagctgct ggtgtgaaaa tcatccaaat tgatgaggct
     8461 gctcttcgtg aaaaattgcc actccgccgt agcgactggt acgaagacta ccttgactgg
     8521 gctattcctg ccttccgctt ggtacactca acagtagcgc cagacacaca aatccacact
     8581 cacatgtgtt actcagaatt tacagatatc atcccagcta tcgataacat ggatgcagac
     8641 gttatttcct ttgaagctag ccgttcaaac cttgaaatct tggacgaact caaagcgaaa
     8701 aacttccaaa cagaagtggg acctggggtt tacgatatcc actcacctcg tgtaccaaat
     8761 gaaggcgaaa tcgacaacac aatcgaagcc atccttgcta aagtgccaag caagaaagtt
     8821 tggatcaacc ctgactgtgg tttgaaaaca cgtggtatcc cagaaacaaa agaaagcttg
     8881 atccgccttg ttgaagcagc taaagctgcg cgtgagaaat tgtaagattg gatttctctc
     8941 catacagtgg ttgttcaaaa agaaatcaac tagaaaaggt tattacatat gtcacgccaa
     9001 acaccgtcac tctcatttga agtgttccct ccaaacccag ccgtgggtaa tgataacatt
     9061 atttcagcct tgcaggatat gcaggagttg gttccccatt ttatcagtgt aactgccagc
     9121 aataataaat ttaatatcaa ggaaacgacg gttcgtttgg ctgactttat ccaaaatgat
     9181 ttggcgattc cgaccattgc ccacttgcca gctatttatc taactaagga caaggttgct
     9241 gaaactattg ctgacttgga caaagttggg gtgcagaaaa tcttggctct tcgtggggat
     9301 attattccag acgtggaacc acaaaaggat ttccgctatg caaccgactt gattgagttt
     9361 atcaaggaac gagcccctca ctttgatatt attggagctt gctatccaga agggcatcca
     9421 gattcgccaa atcagatttc agatattcaa aatcttaaga agaaagtgga tgcaggctgt
     9481 tcgagcctcg taactcagct cttctttgac aatgagcgct tctatgattt tcaggacaag
     9541 tgtatcttgg ctgggattga tgttcccatt catgcaggaa ttatgccaat tctgaatcga
     9601 aatcaggctc tccgactctt gaagacttgt gagaatatcc atcttccacg caaatttaaa
     9661 gccatcttag acaagtatga gcatgaccct gagtcgctca gagcagcagg acttgcctat
     9721 gcagtggacc aaatcgtgga cttggtaact caggatgttg ccggtgtgca tctctatacg
     9781 atgaataatg cttatacagc aaaatacatc catcaagcaa cccatgcctt gtttaatcac
     9841 cagtctctag gataataaaa gcaaaccatt cttctcaggt gaggggaatg gttccttttt
     9901 aatggtaaag acctcacttt ttaagaaaaa tatgataaaa taaattctgt acgtacttga
     9961 tacaaagatg aaggtattaa ataatacaat gcatttaaac tgtaagattt aacgacacga
    10021 gtgagtcaaa tcgatgttat ttgacgagag agttagtaaa gcatttagct aatcgcccga
    10081 tagcgattag cgtgaaagct ttagatactt tagtattaaa cctttctaat gatgtatctt
    10141 gaaaactgaa cttgcctaca actctgtgga aaaagataaa tctccctagg agtagtcgct
    10201 ccgtcgttcg atttcctatc ttcctttgag ttgcttgacg gcttggtatc ttgatgactt
    10261 gaattttggt ctcatttctt aggaaaaaag ataagcttcc tagcactcgt cgagtgtgac
    10321 gtcaccttcc tatttttcta cagaaatgtt cggcaagccg aaccgtccaa aatatcttgt
    10381 gtaattgaac acggccgaaa agctgtgtaa aaaagataaa ctgtcttgtc ttcatcgaag
    10441 acttcgtcag tttcctattt ttactttgct tttgacgtcc ttggtatctt gatctttgta
    10501 ggcaaggcgt ataatttcat caatccaaag gggattaaaa tggcaaaaca agtgtttcaa
    10561 acgacttttg cgggtcgtga gttaattgta gaaactggtc aggttgctaa gcaagcaaat
    10621 ggctctgttg tcgtacgtta cggtgagtca actgtcttga ctgctgccgt tatgtctaag
    10681 aaaatggcaa ctggggattt cttcccactc caagtcaact acgaagaaaa aatgtatgcg
    10741 gctgggaagt ttcctggtgg ctttatgaaa cgtgaaggac gtccttcaac agatgcgacc
    10801 ttgacagcgc gtttgattga ccgtccgatt cgtcctatgt ttgcggaagg tttccgtaat
    10861 gaagtccaag tcatcaatac agtgctttct tatgatgaaa atgcatctgc accaatggct
    10921 gctatgtttg gttcatcttt ggcactgtct atttcagata ttccatttga cggaccaatt
    10981 gctggggtac aagtgggata tgtagatggc caaatcatca tcaacccaag tcaagaacaa
    11041 gcagagcaat ctcttcttga attgacagta gctggaacca agcacgctat caacatggta
    11101 gagtctggtg ccaaagaatt gtcagaagaa atcatgttgg aagcgcttct taaagggcac
    11161 gaagctgtca aagaattgat tgccttccaa gaagaaatcg ttgctgctgt cggtaaagaa
    11221 aaagcagaag tggaattgct tcacgtggat gctgaattgc aagctgaaat cattgcagcc
    11281 tacaacagtg acctccaaaa ggcagttcaa gtagaagaga aattggcccg tgaagctgca
    11341 actcaagcag tgaaagacca agtgactgcc gtttacgaag aaaaatatgc gaaccacgaa
    11401 gaatttgacc gtattatgcg tgatgtggct gaaatcttgg aacaaatgga acacgcagaa
    11461 gtgcgacgtt taattacaga agacaaggtg cgtcctgatg gtcgtaaggt cgatgaaatc
    11521 cgtcctttgg atgcggttgt tgacttcctt cctcgtgtac atggttcagg tctctttact
    11581 cgtgggcaaa ctcaagctct ttcagtcttg accttggctc cgatgggaga aactcaaatc
    11641 attgatggtt tggatccaga gtacaagaaa cgctttatgc accactataa cttccctcaa
    11701 tattctgtag gggaaacagg tcgttacggt gcgccaggtc gtcgtgaaat cggtcacggt
    11761 gcccttggtg agcgtgctct tgctcaagtc ttgccaagct tggaagaatt cccatacgct
    11821 atccgtctag tagcagaagt tttggaatca aacggttctt catctcaagc ttctatctgt
    11881 gcgggaactc ttgcccttat gactggtggt gtgccaatca aggcgccagt agctggtatt
    11941 gctatgggac ttatctcaga tggaaataac tacacagtat tgacagatat ccaaggtttg
    12001 gaagatcact ttggagatat ggacttcaag gttgcaggta ctcgtgatgg gattacagcc
    12061 cttcaaatgg atatcaagat tcaagggatt actgcagaaa tcttgacgga ggctcttgct
    12121 caagccaaga aagcgcgttt tgaaatcctt gatgtcattg aagcaaccat tccagaagtt
    12181 cgtccagaat tggctccaac tgctccgaaa attgatacga tcaagattga tgtggacaag
    12241 attaagattg tcatcggtaa gggtggagaa accatcgaca agattatcgc tgaaacaggt
    12301 gttaagattg atatagacga agaaggaaat gtgtctatct actctagtga ccaagatgct
    12361 attaaccgtg ccaaagaaat tattgctggt ttggttcgtg aagccaaagt ggatgaagtt
    12421 taccgtgcta aagtcgttcg tatcgagaaa tttggtgcct ttgttaacct ctttgataag
    12481 acagatgccc ttgttcatat ctctgagatg gcttggactc gtaccaatcg tgtagaggat
    12541 ttggtagaaa tcggggatga agttgatgtt aaggttatca agattgatga aaaaggccgt
    12601 atcgatgcct ctatgaaggc tcttctacct cgtccgccaa aacctgagca tgatgaaaaa
    12661 ggtgaaaagt ctgagcgccc tcaccgccca cgtcatcaca aggatcacaa acctaagaaa
    12721 gaatttacag aaacaccaaa agattcagag taagaaaagg agaaatgtat ggggtggtgg
    12781 cacgaaacca ttgatatcgt aaaagaaaat gatccagcgg cccgcaccac tttggaggtt
    12841 ttgctgactt atccaggtgt caaggccttg gcggcccacc gtctctcgca ttttctctgg
    12901 aagtacgact tcaaactctt agctcgtatg cacagtcagt tttggcgctt ttggactcag
    12961 attgagattc atccaggagc ccagattgat tcaggtgtct ttattgacca tggttctggt
    13021 ctggtgattg gagagacagc gattgttgaa aaaggcgttc ttctctatca cggagtgact
    13081 ctcgggggaa cagggaaaga ctgtggcaaa cgccatccga ctgtacgaaa gggagccctc
    13141 atatcagccc atgcccaagt tatcgggcct gtggaaattg gtgaaaatgc caaggtcggt
    13201 gctgcagcag ttgtcgtagc agatgtacct agtgatgtga cggttgtcgg tattccggcc
    13261 aagattgtcc gtcttcatgg taagaaagat gagcctgtta ttcacgaagt cgaagaaaaa
    13321 cgggagtatt atgtcaataa actcgagcag gctaaagatg ccagtcacag atcgtctggt
    13381 ttgtagagga tacctatatg attcaagcaa gaaacaagtt aagccaagag gagctatctg
    13441 aggcgaaaaa agtaattaac tgttgccaaa actatgacgg tacctatcgc gatccctatc
    13501 tctctaacat gcttaatttt gacccaaaca tgcccgcctt tttcctttat tatgaaaaag
    13561 gcaaacttgt tggtttatta actgtctatg cagatgacca agatgtagaa gtgacgatat
    13621 tggttcatcc aggtcatcgc cgtcagggga ttgcgcgtgc attgtttact agttttgaga
    13681 gagaaacagc ttctttcccc attcggtcag tcacttttca gacagaacgt atttttttag
    13741 agaatcatcc tgactttgcc agtaactggg gactaatcga ggatgaagag acagaaactt
    13801 ggttaggtaa ggatagaaga ccttatccgt tagcaaatgt ttcccatctt gaagttttgt
    13861 tagcagatag ttcgtatcag gatcaaatta gtcaattaaa atttcaggca ttttcagagg
    13921 aacatgaatc gagagaagtt gtggatagat atgtcgctga agctctgaag gatccagaaa
    13981 gtcgcttata tattttgtta aaagacggtc aggttattgg gacttgcacg gtagatttat
    14041 cgactaatac gaattacttc tacggtttag caatatcgga acctgaacgt gggaaaggct
    14101 atggaagcta cttagcaaaa tccctcgtca accaactaat tgagcaaaat gacaaggaat
    14161 ttcagattgc agtggaagat agcaatgtag gtgccaaacg tttgtatgaa aaaattggct
    14221 ttgtcaaaca gacacaggtg gtttatctga atgagaaagg agcaagggat tccgaagtgt
    14281 agagacattc ggactgaaat ttacttgagc tcttagttta gtgatgaaac taacagttct
    14341 tggattttag ctttcctgat tatgatttat gattaaaatc tatgacacca tgtctcgtga
    14401 tttgcgagaa tttgtcccga ttgaggacgg caagatcaag atgtatgttt gtgggccaac
    14461 cgtgtacaac tatattcacg tgggaaacgc ccgttcgacg gtagcttttg atacgattcg
    14521 tcgctatttt gagtaccgtg ggtataaggt tgcctatatt tccaatttta cagatgtgga
    14581 tgataagatt atcaaccgtg ccagggaaga aggcatcaca cctcaggagg ttgcggataa
    14641 gtacatcgct gcctttcgtg aggatgtgac ggccttaggc gtgaaacctg cgactcgcca
    14701 tccgcgtgta gtggagttta tggcagacat catccgtttt gtggaagact tgatcgagaa
    14761 aggctttgcc tatgagagtc aaggggatgt ctatttccgt gtagaaaaat cccacaacta
    14821 tgctaaattg gctaataaaa ccttggaaga tttggagcta ggtgcttcag gtcgtaccga
    14881 tgaagaaacg gctcgtaagg aaaatcctgt agattttgct ctatggaaat cttccaaacc
    14941 aggtgagatt tcttgggata gtccttgggg acctggacgt ccgggttggc atattgagtg
    15001 ttcagtcatg tcgacagaga ttttaggtga taccattgat atccacggtg gtggagctga
    15061 cctagagttt ccacaccaca ccaatgaaat tgcccaatct gaagctaaaa caggcaaggc
    15121 ttttgctaac tactggatgc acaatggctt tgtcaatatc gacaatgtca aaatgtctaa
    15181 gtccttgggt aacttcatta cggtacacga tgcccttaaa actcttgatg ggcaagtact
    15241 tcgtttcttc tttgcgaccc agcactaccg taagcctatc aacttcacgg aaaaggcagt
    15301 tcgcgatgcc gagaccaatc tcaagtatct gaagaatact tacgaacaac catttacagg
    15361 aaacgtggat gcccaagagt tacaaaactt taaagataag tttgtagcag ctatggatga
    15421 agattttaat gctgccaacg gtatcacagt tgtctttgaa atggccaaat ggatcaactc
    15481 agggaactat gatgcaagtg tcaagcaagc tcttgcagat atgttagaaa tttttggaat
    15541 tgtctttgtt gaggaagttt tggatgcaga gattgaagac ttgattcaaa aacgccaaga
    15601 ggcgcgtgcc aatcgagact ttgagacagc agaccaaatc cgtgaccaat tggttactca
    15661 aggaattaag ctccttgata ccaaggatgg agtgaggtgg acacgtgatt gatgtcaatc
    15721 tcattaacgg gattgcgcta gcctttgagg gggatgcggt gtattctatg tatattcgcc
    15781 gtcacctcat cctcaaaggt atgaccaaac ccaataaact ccatcaagaa gcaaccaagt
    15841 atgtgtcagc caaggctcag gctcgcctga ttgctctcat gttggaggag caggtcctaa
    15901 cggaaaaaga agaagaaatc tacaaacgtg gccgcaatac caatagccac acaaaggcta
    15961 aaaatgcaga tgtcgtgact tatcgtatgt ccacgggatt tgaagcggtt atgggctatc
    16021 tccatatgac tgagaatctg gaacgtcttg agagtttggt ttcatggtgc atccaaaaag
    16081 tggagggcta gaacatgagg gaaaaagaac tacaagactg gtttcctgag gctcggattt
    16141 cagaccaacc agtagagaaa gagggctatc tcacgctccc tttagcttct cagcagtgga
    16201 ttttgctgga ggaagctggg ctcagcgagc gtgaaaagca gttggttgcc cttttgaccc
    16261 agcaggagca ggctcgttcg ctaaaccctt ggtattccta tctggttgaa ggcaagggac
    16321 aggcaccgca agtttttaaa aagattcagt tggtttattg ccatctttct tattttcagc
    16381 aggaaaatct ggcttcttgg ctagatatga tgcggactct ttttccgaat tgtcagacag
    16441 tgctacaggt cggagctcag gattatgttt tcgtgcttca acaagacaaa tacacttctg
    16501 taagagatat tttaagtgat acgattgaag cggttgagta tgactttgga cttcgtcttt
    16561 ctatcatgtt gggtcaggtt tggtctcaga caggacatca agccctatca gacttaatca
    16621 aagctgagcg ggatttgttc aagacatggt ggcgtcaggg tcaccaaggt gttcatactt
    16681 tttctcagct ctatctttgg agtatgggag aaagactcgt ggacttgaag ccaattaaag
    16741 aatgtctaca ccagatgatt ttggatcaag atcagattca ggaaatcatt ctctctcttt
    16801 gggaaaatag tgctgttctc actaaaacag cccagcaact ctatctgcac cgcaattctc
    16861 tccaatacaa gattgataaa tgggaagagt tgacagggct tcagttgaaa gagttgaccg
    16921 acctgacctt gtgttatcaa ttgattttag gttctttgtc aactatagtt ggtttgtaaa
    16981 gaagttaata tttggagaag aggattgcca tcttctccat tt
//
LOCUS       DAVKQB010000046        16946 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKQB010000046 DAVKQB010000000
VERSION     DAVKQB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.046"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..572)
                     /locus_tag="VW528_001617"
                     /pseudo
     CDS             complement(1..572)
                     /locus_tag="VW528_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(727..1437)
                     /gene="treR"
                     /locus_tag="VW528_001618"
     CDS             complement(727..1437)
                     /gene="treR"
                     /locus_tag="VW528_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9912418.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            1622..3589
                     /gene="treP"
                     /locus_tag="VW528_001619"
     CDS             1622..3589
                     /gene="treP"
                     /locus_tag="VW528_001619"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9912419.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFVYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            3767..5392
                     /gene="treC"
                     /locus_tag="VW528_001620"
     CDS             3767..5392
                     /gene="treC"
                     /locus_tag="VW528_001620"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9912420.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGIKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQTLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            5648..5896
                     /locus_tag="VW528_001621"
     CDS             5648..5896
                     /locus_tag="VW528_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9912421.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            6103..6897
                     /gene="racE"
                     /locus_tag="VW528_001622"
     CDS             6103..6897
                     /gene="racE"
                     /locus_tag="VW528_001622"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9912422.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            6894..7865
                     /locus_tag="VW528_001623"
     CDS             6894..7865
                     /locus_tag="VW528_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9912423.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYQTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            7841..8362
                     /locus_tag="VW528_001624"
     CDS             7841..8362
                     /locus_tag="VW528_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9912424.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            8359..8820
                     /locus_tag="VW528_001625"
     CDS             8359..8820
                     /locus_tag="VW528_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9912425.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            8811..9551
                     /gene="xerD"
                     /locus_tag="VW528_001626"
     CDS             8811..9551
                     /gene="xerD"
                     /locus_tag="VW528_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9912426.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQRRIVT
                     IPTALLSELEPLMGQTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            9551..10279
                     /locus_tag="VW528_001627"
     CDS             9551..10279
                     /locus_tag="VW528_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9912427.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            10298..10867
                     /locus_tag="VW528_001628"
     CDS             10298..10867
                     /locus_tag="VW528_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9912428.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQALDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            10854..11576
                     /locus_tag="VW528_001629"
     CDS             10854..11576
                     /locus_tag="VW528_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9912429.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            11576..11818
                     /gene="yidD"
                     /locus_tag="VW528_001630"
     CDS             11576..11818
                     /gene="yidD"
                     /locus_tag="VW528_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9912430.1"
                     /translation="MKRILIALVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(12043..13008)
                     /locus_tag="VW528_001631"
     CDS             complement(12043..13008)
                     /locus_tag="VW528_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9912431.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETSTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(13104..13856)
                     /locus_tag="VW528_001632"
     CDS             complement(13104..13856)
                     /locus_tag="VW528_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9912432.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINNKKICIYS"
     gene            complement(13853..14809)
                     /locus_tag="VW528_001633"
     CDS             complement(13853..14809)
                     /locus_tag="VW528_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9912433.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(14799..15758)
                     /locus_tag="VW528_001634"
     CDS             complement(14799..15758)
                     /locus_tag="VW528_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9912434.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFISSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(16181..16882)
                     /gene="galE"
                     /locus_tag="VW528_001635"
     CDS             complement(16181..16882)
                     /gene="galE"
                     /locus_tag="VW528_001635"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9912435.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMDQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
BASE COUNT         4774 a         3369 c         3887 g         4916 t
ORIGIN      
        1 accttcaaga acagtgatga tatcgagctt ttcaaaatct tgcgcaataa agctcatctc
       61 catctcccga ttgaaacagt cactccccgg actgtttcaa cgtcccaaga cataatctta
      121 ggcagatttc taaaattaca ctcaaagtgg aagtcattga gctttcgaat gacagttgaa
      181 gttgagatgg aaagctgatg ggcaatatca gtcatagaaa tcttttcaat taacttttga
      241 gcaatctttt ggttgatgat acgagggatt tggtgattct tcttgacgat agaagtttca
      301 gcaaccataa ttttagaaca atgatagcac ttgaaacgac gctttctaag gagaattcta
      361 gtaggcatac cagttgtttc aagataagga attttagaag gtttttgaaa gtaatatttc
      421 ttcatttgac ttccgcactc aggacaagat ggagcttcat agtccagttt ggcgatgatt
      481 tccttgtgtg tatccttctt gatgatgtct aaaatctgga tattagggtc tttaatgtct
      541 agtaattttg tgataaaatg taattgttcc atatgattct ttctaatgag ttgttttgtc
      601 gcttttcatt ataggtcata tgggactttt tttctacaca aaataggctc cataatatcc
      661 ataggggggt tacccactac aaatattata gagccgaaaa aggctgagat ctggtgtctc
      721 agtcttttat ttcttgcgtt ttgcaaaatc tacaaatcta aatttttcta acttatggcg
      781 actttcggta aactgaaatt ggcgtccgtc ttggagatag acttttgatt taatcgaaac
      841 gacataaggg tctttgccaa tgtccatgag aatcttgtct cggtcgcttg agtggtcaat
      901 ggtgatttcc ttctgagcat aatcaataag gagtttgagg ccattttcta tataagaata
      961 gatagactgc tcagcaattt ggcgagtgag atttggaatt agttccatat ccagatagtc
     1021 tgtatccaga acggatacca gatcatccac cacacgctgg cggaccacct tccaaaccat
     1081 ccgaaactct gggaaaccgg ttatcagtga ggatttttta tcaataataa tcttgtccag
     1141 actgaccacg ttggttttag agcgcagtcc aagttcttta actagttctt ggtagctggt
     1201 taggttggat acagggaaat tgacggtttc ttctttgacg acttgagaac cttgccctct
     1261 tatctttttg atcaatcctt cctcttggag gagagacagg gcctttcgga cggtatcacg
     1321 actcacttga tattgctcca taaggtcgtg ctcgctagga aggaaatctc cgacagcgta
     1381 agtctcgttt tgaatggttt cttggatttg cttaaataat tgttggtatt tcttcatttt
     1441 gcctgcttaa ataggttttt agtctagttt aatgaacacc tgtatctatt ttaccataca
     1501 agtgctagaa aatttacaaa aaaacaccct ttttatacca aacaagttgc agacaagttt
     1561 ggtatcgttt acaatatact tatcaaatca aacttgcttt gacaagtata aggagaatca
     1621 aatgggaaaa tttgaacaag aagccaaaga tctgcttcag gcaattggag gcaaagaaaa
     1681 tgtgactgcc gtcacccact gtgcgacacg gatgcgcttt gttttaggag atgataagaa
     1741 ggctaatgtt aaagctatcg agtcaattcc agctgttaaa ggaactttta caaatgcagg
     1801 tcaatttcag gtaatcattg gaaatgacgt gcccatcttt tataatgatt ttacagccgt
     1861 ttcaggtatt gagggtgttt ccaaagaagc agccaagtct gcagctaaga gtaatcaaaa
     1921 cgtggtccaa cgtgttatga ccactctggc ggagattttt actccgatta ttccagcctt
     1981 gatagtcgga ggattgatcc tcggtttccg taatgtcttg gaaggtgtcc attggtcgat
     2041 gttggatggc aagaccatca cagaatcctc tcagttttgg gcaggtgtca atcacttcct
     2101 ctggttgcct ggtgaagcta tcttccagtt cttaccagta gggattactt ggtctgtttc
     2161 tcgtaagatg ggaaccagcc aaattttggg aattgttctc ggaatctgtt tggtatcgcc
     2221 tcagttgctc aatgcctatg cggttgcttc aacgccagca gctgatatcg cggcaaactg
     2281 ggtttggaat tttggctatt ttactgttaa tcgtatcggt taccaagccc aagttatccc
     2341 agccttgctt gcaggtttga gtctgtctta tcttgaaatc ttctggcgca agcatatccc
     2401 agaagtcatt tctatgattt ttgtaccttt cttgtcattg attccagcct tgattttggc
     2461 tcatactgtt ttgggaccaa tcggttggac aattggacaa ggactttcat cagttgtctt
     2521 ggcaggttta actggtccag ttaaatggct cttcggtgca atttttggtg ccctctacgc
     2581 tccatttgtc atcacaggtc tgcaccatat gaccaatgcc attgatacac aattgattgc
     2641 ggatgctggt ggcactgccc tatggccaat gattgctctt tctaatattg ctcaaggctc
     2701 agccgtgttt gtctattatt tcatgcatcg ccatgatgag cgtgaggctc aggtttcact
     2761 tcctgcaacc atttcagcct atctcggtgt tacagaacca gctctttttg gggttaacgt
     2821 aaaatatatt tatccatttg ttgctgggat gactggttca gcccttgcag gcatgttatc
     2881 cgttactttt aatgtaactg cggcttctat tggtatcggt ggtttgccag gtattctctc
     2941 tattcaacct caatacatgc tgccatttgc aggaactatg ctagttgcga ttgttgttcc
     3001 aatgctcttg actttcttct tccgcaaggc tggtctcttt acaaaaacag agggcgatac
     3061 gaacttgcag gcagaattcg ttgctcaaga agaagcagaa tttgtgaacc atgaaccagt
     3121 agaacttact tcggtagaaa ttatcagccc actaactggc caagtgaaag aattgagtca
     3181 agcgacggat cctatttttg catcaggtgt catggggcaa ggtctagtca ttgaaccaag
     3241 ccaaggtgag ttgacctctc cagttaatgg gacagtgacg gttcttttcc ctaccaagca
     3301 tgccatcggc attgtctctg acgagggagt tgaattgctc atccacatcg gtatggatac
     3361 agtaggtctt gatggcaaag gttttgaaag tcttgtagtc caaggagatc acgttacagt
     3421 tggtcagcaa ctgattcgtt ttgatatgga tgtcattaag gctgcaggtc tggtgacaga
     3481 aactcctgtt atcatcacca accaagatgc ttatacagcg actattcccg gaacttatcc
     3541 gacaacgatc caagctggag catctctcat ggtcgctaca cgaatctaat actcttcgaa
     3601 aatctcttca aaccacgtca gctctatctg caacctcaaa atagtgtttt gagcaacctg
     3661 tggctagctt cctagtttgt tctttgattt tcattgagta taattgaaat agcacaaata
     3721 gggacggaag actgcttcct cccttttatc agaaaaggag aaacagatga cacttgataa
     3781 aggaaaagta gtctatcaaa tctatccaaa atcttacaaa gacaccactg aaaacggtat
     3841 tggggatttc cgtgggatta tcgaaaaaat tccctattta gccaaactag gtgtggatat
     3901 ggtctggctc aatccattct atccaagccc tcaacgggat aatggttacg atatttcaga
     3961 ttatatggca gtggatcctc tttttggtga tatggctgat tttgaggaaa tggtgtgtgt
     4021 cggtaaagag cacaagattg actttatgct ggacatggta cttaatcatt gttcgacaga
     4081 gcatgaatgg tttcagaaag ccctagctgg tgataagtat tatcaagact ttttcttcat
     4141 ccaagaccaa ccaacagatt ggcagtctaa gtttggtggt tctgcatgga cgcctttcgg
     4201 ggatactggg aaatactatc ttcacctatt tgatgagacc caggctgacc ttaactggcg
     4261 caattccaat gtccgtaagg agcttttcaa ggttgttaat ttctggcgcg acaagggtat
     4321 caaaggtttc cgatttgatg tgatcaattt gatcggtaag gacgaggttt cagtggactg
     4381 tcctgaaaat gaagggaagc cagcttatac agacaaaccc atcgttcata actatttgcg
     4441 tatgatgaac caagccactt ttggatctga cgatagcttt atgacagttg gggaaatgtc
     4501 ttctaccacc atggaaaact gtgtcctcta ttcgtcgcct gatcgtcagg aattatccat
     4561 gacctttaat tttcatcacc tcaaggtgga ctataaggat ggacaaaagt ggaccttggc
     4621 tccctttgat tttgaagagt tgaagagtct ttaccatagt tggggtaagg aaatgagtga
     4681 taaagacggt tggagtgccc tcttttggaa caatcacgat caaccacgtg ccttgaaccg
     4741 ttttgtcgat attcaaaact tccgcaagga aggagccacc atgctagcgg ctagcattca
     4801 cctgtcacgt gggacacctt atatctatat gggtgaagaa atcgggatga ttgacccaga
     4861 ctatgattct atggctgatt atgtggatgt cgaatcgctt aatgcctatc agacgctctt
     4921 agaagaagga aagagtcagc aagaagcctt ccagattatt caagctaagt cgcgtgataa
     4981 ttcacgcatt cccatgcagt gggatgcttc ggaaaatgca ggattttcaa caggcactcc
     5041 atggttgaaa gctggtaaat cctataaata catcaatgta gaaaatgaaa tccagggtcc
     5101 gattttcacc ttctatcaag acttgattcg actgcgtaag gaaatgccaa tcatttcaga
     5161 aggaagttac aaaccagcct ttgaagatag caaacaagtc tacgcttttg aacgccagtt
     5221 tgaggatcaa aagttactag tgctcaataa cttttatgct aaagaagtgg aaatcgactt
     5281 gccagcagtc taccaaaatg gacagatttt gatatcaaac tatgaagatg cagaagtgtc
     5341 tgagaaaatt ctactcaaac cctatcaaac actggctatt tatgtaaatt aatactcttc
     5401 gaaaatctct tcaaaccgtg tcagttttat ctgcaacctc aaagcggtgc tttgagcaac
     5461 ctgtggctag cttcctagtt tgctctttga ttttcattga gtataaactt agcaaggaga
     5521 cctctttttc agaaaagcag ggttcttttt gctgtctatt ccatccctga aagtgtagaa
     5581 caaactggtc agggtggcta gtttgtggta taatgaaagg gattcaaaaa gaaacaggag
     5641 aaaaatgatg gatttacttt tagcaattgt attgattgtg ctagcttttc taggaggagc
     5701 tcttggagga atgtacttgg ttcgtaagca aatcgaaaaa gaatttgctg acaacccacg
     5761 tttgaatgct gaagcagttc gtactctttt gagtgcaaat ggtcaaaaac caagcgaagc
     5821 taaggtacaa caagtttacc accaaatcat ccgccaacaa aaggcagccc ttgctaacaa
     5881 taaaaagaaa aaataaataa ggaaaagtct gggatgaaag ttccagactt ctcactatgt
     5941 tggttatggt ttagggatga gactttttcc ttgcattcta ttgatatttg attatgatta
     6001 agagagaggt agttgacatt ctatcaagct gtcggtcagt tcgttcagta caaggaatca
     6061 taatgaacga tcaatcagaa aaaagactag aaagaagact gtatggataa tcgaccaatt
     6121 ggttttttgg attcgggtgt cgggggcttg accgttgtgc gcgagctcat gcgccagctt
     6181 ccccatgaag aaatcgtcta tattggagat tcggcgcggg cgccctatgg cccccgtcct
     6241 gctgagcaaa ttcgtgaata tacttggcag ctggtcaact ttctcttgac caaggatgtc
     6301 aaaatgattg tcattgcttg taacactgcg actgcggtcg tctgggaaga aatcaaggct
     6361 caactagata ttcctgtctt gggtgtaatt ttgccaggag cttcggcagc catcaagtcc
     6421 agtcaaggtg ggaaaatcgg agtgattgga acgcccatga cggtacaatc agacatatac
     6481 cgtcagaaaa tccatgatct ggatcccgac ttacaggtgg agagcttggc ctgtcccaag
     6541 tttgctccct tggttgagtc aggtgccctg tcaaccagtg ttaccaagaa ggtggtctat
     6601 gaaaccctgc gtcccttggt tggaaaggtg gatagcctga ttttgggctg tactcattat
     6661 ccactccttc gccctattat ccaaaatgtg atggggccaa aggttcagct catcgatagt
     6721 ggggcagagt gcgtacggga tatctcagtc ttactcaatt attttgaaat caatcgtggt
     6781 cgcgatgctg gaccactcca tcaccgtttt tacacaacag ccagtagcca aagttttgca
     6841 caaattggtg aagaatggct ggaaaaagag attcatgtgg agcatgtaga attatgacaa
     6901 ataaaattta tgaatataag gatgaccagg actggtatgt tgggtcttat agtatttttg
     6961 gtggcgttaa cagtttgagc gactatcaga cagattttcc tctgtttgaa ttctccaaaa
     7021 tatttggaga tgaagagtat ggtttcccgc tttcagttac tgttttacgc tatggttcta
     7081 cctaccgttt gttctccttt gtggtagata tgcttaatca agaaatggga cgaaacttgg
     7141 aagttattca acgtcatggg gccctgctct tggttgaaaa tgggcaactc ttgtatgtag
     7201 aattgcctaa agaaggggtc aatgttcatg atttctttga gacaagcaaa gtcagagaaa
     7261 ccttgttgat tgcgactcgt aacgaaggta aaaccaagga attccgagct atctttgata
     7321 agttaggcta cgatgtggaa aatcttaatg actaccctga cctgcctgaa gtagcagaaa
     7381 caggtatgac ctttgaggaa aatgcccgcc ttaaggcaga aaccatttct caattaacgg
     7441 gcaagatggt tttggcagat gattctggtc tcaaagtcga tgtccttggt ggcttaccag
     7501 gcgtctggtc agctcgtttc gcaggtgtgg gagcaactga ccgtgaaaat aatgccaaac
     7561 tcttgcacga attggccatg gtctttgaac tcaaggaccg ctcggctcag ttccacacaa
     7621 ccctagtcgt agccagccca aataaggaaa gtttagttgt tgaagcagac tggtcaggtt
     7681 atattaactt tgaacctaag ggtgaaaatg gctttggcta tgatcccctc ttccttgtag
     7741 gagagacagg tgagtcatca gctgaattaa ccctggaaga aaaaaatagt caatctcacc
     7801 gtgccttagc cgttaagaaa cttttggagg tatttccatc atggcaaagc aaaccatcat
     7861 tgtaatgagc gattcccatg gcgatagctt gattgtggaa gaagtccgtg atcgctatgt
     7921 gggcaaagtc gatgccgttt ttcataacgg cgattctgaa ctacgtccgg attctccact
     7981 ttgggagggc atccgcgttg ttaaagggaa catggacttc tacgccggct acccagaacg
     8041 tctggtgact gagcttggtt cgaccaagat tatccaaact catggtcact tgtttgacat
     8101 caatttcaac tttcaaaagt tggactactg ggctcaggag gaagaggccg ctatctgcct
     8161 ctatggtcac ttgcatgtgc caagtgcttg gttggaaggc aagatcctct ttctaaatcc
     8221 aggctctatc agtcaaccac gaggtaccat cagagaatgt ctctatgctc gtgtggagat
     8281 tgatgatagt tacttcaaag tggacttttt gacacgagat cacgaggtgt atccaggttt
     8341 gtccaaggag tttagccgat gattgccaag gagtttgaga ctttcttgtt ggggcaggag
     8401 gaaacttttt tgacccctgc taaaaatcta gctgtgttga ttgatactca caatgcggat
     8461 catgcgaccc tcttgctcag tcagatgacc tatacccgtg ttcccgttgt gacagatgaa
     8521 aaacagtttg ttgggacgat tggactcaga gatattatgg cttatcagat ggagcatgac
     8581 ttgagccaag aaatcatggc ggatacggat atcgttcata tgacaaaaac ggacgtagcg
     8641 gttgtttcgc ctgatttcac cattacggag gtcttgcaca agctagtaga tgagtccttc
     8701 ttaccggttg tggatgcaga gggtattttc caagggatta ttacgcgcaa gtccatcctc
     8761 aaggccgtta atgccctctt gcatgacttt agtaaggaat atgagattcg atgccaatga
     8821 gagacaggat ttcagccttt ttagaggaaa agcagggctt gtctgtcaat tccaagcagt
     8881 cctataagta tgatttggag caatttttag acatggtagg tgagcggatt tctgagacca
     8941 gtctcaagat ttaccaagcc cagctagcca atctaaaaat cagcgcccag aagcgaaaga
     9001 tttcggcctg taaccaattt ctatactttc tctatcaaaa aggagaggtg gacagctttt
     9061 accgcttgga attagccaaa caagctgaaa agaagacgga aaagccagag attctatacc
     9121 tagactcttt ttggcaggaa agcgaccatc cagagggccg cttgctagcg ctcttaatcc
     9181 tagaaatggg gctcttgccc agtgagattt tagccatcaa ggttgcggac atcaatctgg
     9241 attttcaggt gttgcgaatc agcaaggctt cccaacggag gattgtcacc attcccacgg
     9301 ccttgctttc agaattggaa cccttgatgg ggcagaccta tctttttgaa agaggaggga
     9361 aaccctattc tcgtcagtgg gcctttcgtc agttagaatc ttttgtcaag gagaaaggtt
     9421 ttccatcctt atcagctcaa gtcttacgtg aacagtttat tctaagacaa atagaaaaca
     9481 aggtcgattt gtacgaaatt gcaaaaaaat taggattaaa aacagtcctg accttagaaa
     9541 aatatagata atggatatta aattaaaaga ttttgaagga cccctggact tactcttgca
     9601 tctggtttct aagtaccaga tggatatcta cgatgtgccc attacggaag tcatcgaaca
     9661 gtatctagcc tatgtctcaa ccctgcaggc catgcgtctg gaagtgacgg gtgagtacat
     9721 ggtcatggct agtcagctca tgctgattaa gagtcgtaaa ctccttccga aggtagcaga
     9781 agtgacagac ttgggggatg acctggagca ggacctcctc tctcaaatcg aagaatatcg
     9841 caagttcaag ctcttgggtg agcacttgga agccaagcac caagaacggg cccagtatta
     9901 ttccaaagcg ccgacagagt tgatttacga agatgcggag cttgtgcatg acaagacgac
     9961 cattgacctc tttttggctt tttcaaatat cctagccaag aaaaaagagg agtttgcaca
    10021 aaatcacacg actatcttgc gggatgagta taagattgag gacatgatga ttattgtgaa
    10081 agaatccttg attggacgag atcaattgcg cttgcaggat ttgttcaagg aagcccagaa
    10141 tgtccaagag gtcatcaccc tctttttggc aaccctagag ttaatcaaaa cccaggagct
    10201 gatcctcgtg caagaggaga gttttggaga tatctatctc atggaaaaga aggaagaaag
    10261 tcaagtgcct caaagctaga cttgatagag aggaaagatg agtactttag caaaaataga
    10321 agcgctcttg tttgtagcgg gtgaagatgg gattcgggtc cgccagttag ctgaactcct
    10381 ctctctgcca ccgacaggca tccagcaaag tttaggaaaa ttagcccaga agtatgaaaa
    10441 ggacccagat tccagtttgg ctttgattga gacaagtggt gcttatagat tggtgaccaa
    10501 gcctcaattt gcagagattt tgaaggaata ctctaaggcg cctatcaacc agagcttgtc
    10561 tcgggctgcc cttgagacct tgtccattat tgcctacaaa cagccgatta cgcggataga
    10621 aattgatgcc atccgtgggg ttaactcgag tggagccttg gcaaagttgc aagctttgga
    10681 cctgataaag gaagacggga aaaaggaagt attggggcgc cccaacctct atgtgactac
    10741 ggattatttc ctagattaca tggggataaa ccatttagaa gaattaccag tgattgatga
    10801 gcttgagatt caagcccaag aaagccaatt atttggtgaa aggatagaag aagatgagaa
    10861 tcaataagta tattgcccac gcaggtgtgg ccagtaggag aaaagcagaa gagctgatca
    10921 agcaaggttt ggtgacagtt aacggccaag tggtgcgtga actagcaacc actatcaagt
    10981 caggcgacaa ggtcgaagtt gaaggtcaac ctatctacaa cgaagaaaag gtctattatc
    11041 tgcttaacaa accacgcggt gtcatttcca gtgtaacaga tgacaagggt cgcaagacgg
    11101 ttgtcgacct cttgcccaat gtcaaagagc gcatttaccc tgtgggtcgt ttggactggg
    11161 atacatcagg agtcttgatt ttgaccaatg atggggactt tacagacgag atgattcacc
    11221 ctcgtaatga gattgacaag gtttatgtcg cgcgtgttaa aggtgtggcc aataaggaca
    11281 atcttcgccc cttgacccgt ggtcttgaga ttgatggtaa gaaaaccaag ccagctgttt
    11341 atgaaattct caaagtggac ccagtcaaaa atcgctctgt ggtgcagttg accatccatg
    11401 aagggcgtaa ccatcaggtt aaaaagatgt ttgaagctgt tggtctccaa gtagataagt
    11461 tgtctcggac tcgtttcgga cacctagact tgacaggact ccgtccagga gaatcccgtc
    11521 gtcttaataa aaaagaaatc agccaactac acaccatggc tgtaactaag aaataatgaa
    11581 acgaatttta atagcgcttg tgcgctttta ccaacgtttt atctcaccag tctttccacc
    11641 ctcttgtcgc tttgagctga cttgttccaa ctacatgatt caggctattg aaaaacatgg
    11701 ttttaagggg gtattgatgg gcttggctcg gattttacgt tgtcatccct ggtcgaaaac
    11761 aggtaaggac cccgttccag accacttttc ccttaaacga aatcaagaag gggaatgagg
    11821 tggggtaaat agatttcaaa atgataaaaa cgcatcctat caggtttgag tgaacttgat
    11881 aggatgcgtt ttagaatgtc aaaattttat actcttcgaa aatctcttca aaccgcatca
    11941 gctttcatct gcaacctcaa aacagtgttt tgagcaacct gcggctagtt tcctagtttg
    12001 ctctttgatt ttcattgagt attaaattga gtttgaagtg gcttatttca aagctttttg
    12061 tatgtcttca atcatgagtt ttgttgattc aagtccgcct ccgcttagat accagaggtc
    12121 tggtgttagt tggataatct taccattttt agcagcaggt gtttcagcga taagggcatt
    12181 ttctaggaca ccgtcgttgc tagagttgtc cccaccgatg gcaagggtac ggttgatgac
    12241 aaagaggatg tcagggttga tttctttgac actttcaaag ctgacttctt gtccgtggcg
    12301 tgagtcttca aattttgtat cagttggttt gaatttcaag gtttggtaca agaaagagaa
    12361 acgagatttg gcaccaaagg ctgccatttt tccttcatta aggaggatcg caagggcttt
    12421 tttgtcagag ctttcatttt tagtagcgac ttcttggatg ctcttgtcta gcttggccaa
    12481 ttcttccttg gctttttgtg tactagtttc gccgaaggca cttgctaagg attcgatatt
    12541 agccttggta gaagtccagt agtcatcctt gcttgcttgg aagagaacgg ttggggcgat
    12601 ttctttgaat ttgtctacaa atttttgtgt acgtggtgaa gcgataatca aatcaggctc
    12661 aagggcggcg atagcttcta aatcaggttc tttcatagaa ccaacatttt tgacagttcc
    12721 cactaggtct tttagataag tcggaacagt ttttgtaggc attccgacga tatttttttc
    12781 aaatcctaaa gcgcgaatag tatccgcagc gccgaggtca aaggtcacaa tcttttcagg
    12841 aactttggaa agtttgacct cgtccagtga acttttaatg gttacctctg ttggagcaga
    12901 gctactggtc tctgtctgac tagtgcttga gtttgtacta catgcaccaa gtaggagcaa
    12961 gaagctggcc actagggcag tgaaataaag tttaagggat gttttcataa tttctccttt
    13021 ttaaaatgtg ataacgattt agggagtctc ttaatcttat tgactaagag actgaaggtt
    13081 ctctaacttg agcttttatg ttactagcta tagatacaga tctttttgtt attgatatca
    13141 gctagcgtga tgggaatctc ataaagttga ctgagcaggt cagcctgcat gatttgatcg
    13201 gttcttccct tgctaaagac ctggccgtcc ttgaaggcga caatttcatc tgcatactga
    13261 ctggccatgt tgatatcgtg gaggacgatg ataatggtct tgccgagttc ctccaccagt
    13321 cgtcgaagaa tctgcatcat gctgacgctt tgcttgatat cgagattgtt gagtggttcg
    13381 tccagcaaga taaagtccgt atcctgggcc agtaccatag cgataaagac gcgctggagt
    13441 tgcccccctg acaggctatt gatgtagcgg tcttttaagt tggtcagttc caaatagttc
    13501 agagtttctc ggattttttc ccagtcttct gatctaagtc gacctcggct gtagggaaaa
    13561 cgtccaaaac tgaccagttc ttcaacagtc aatttggctt ggtaattgat tttctgtttt
    13621 aggatggtta gttcttgggc cagttcttgc gaattccagc tctcgatttc acgtcctttg
    13681 atactgagaa ctccctgatc tttcttggtt agtctgctca tgatggagag gagagtcgat
    13741 tttccagcac catttggacc aataaaggct gtcagttttt gaggactgac ttcaagcgaa
    13801 atgccttgca aaatatcctg tttttgaatg gatttgtcaa tgttttccag tttcactgac
    13861 gagccctcct atatagtaag ataaagaata agaagccacc cacactctca atgatcatac
    13921 tgatacgaat ttccagtgca aagactcgtt caatcaaggc ttgccccaag gttaagctaa
    13981 taaatccaac cagaatggcc actataaaga gtaacttgtg ctgatagtct ttgacaatca
    14041 ggtaggtgag gttggccagc ataaagccga agaaggccat aggtcctacc aaggcagtgg
    14101 ccgttgaggt caaaagcacg attccccaga ggagctcttt ctgttctttt tcaacatcga
    14161 gtcccaatat ctgagccgtt tctctttgca ggtgcaagac atctagaacg actgcttttc
    14221 gaaagaaaaa gattgtcaaa gcgaggatga tcagagaacc gatggctagg atggaagtgt
    14281 taagatgttg aaaggaggca aaaagactat tttgcagttt atcgtattcg tttggatcca
    14341 ttaggacttg aaggaaggtg ctgatatttc gaaagagact tctgagcgct agacagatca
    14401 gcaggatgaa gaccaggtct tgcttcatca gtgtcttcaa gtaaccttgt aaggcgagaa
    14461 agaagaggga ctggacaaga agtaagacta ggaattctaa gataggggat ttgccaagtt
    14521 gaagaaactt gctttcaaaa accagtagta gggtttgtag taggacgtag aaggattcaa
    14581 ttcccaaaat actaggcgtc aggaagcgat tttccgtcag ggtttgaaaa ctaatggtcg
    14641 aaatcccagt cgcgatggct accaagagat aaacgatgat cttttgggaa cgcaacttcc
    14701 aagcaaaggc tgacaagtga gtgatgggcc aaaagtagag aagacaagct ccgatggcaa
    14761 gaataatgag aatccagaag agcttggtat gtttgctttt agtctgcatc ttttcgtccc
    14821 cctctccaga gaagtaggat aaagacgaga ctaccgatga ttcctagcaa gagactgaca
    14881 gacaactcat agggcctaat cagaactcgg gataggatat cgcaagccag aactagattg
    14941 gcaccaacca gtgcgaccat gagtttggtt tgacttagat tatctccata gcgcttgcga
    15001 acaagattgg gaacgataac tccgagaaat ggtaagccac ccacggtaat catggtgacg
    15061 cttgtcgtta gcgccaccag aaagagggcc agtttttcaa gtagggagta ggaaatcccc
    15121 aaactctcgc tggtttcttt ccctagattc atgatggtga aggtttggga taatttccaa
    15181 acggttatca ggatgatgag gcctaagaag agccactcat actgatgggt ctgaatcatg
    15241 gagaaggagc cctgggtcca ggcagtcata ctctgaacca gattgaaacg ataggcgata
    15301 acttctgtga ctgagccgat aatcccgcta tagatgatcc caatcagagg caacatccac
    15361 ctttccttta cagtaaaaat ggtcataaag gctaggaaga agagggtgaa tacgatggat
    15421 gaaataaaag cgaagagcat cttttgggtc agactagccg atggaaagac aaaaaggctc
    15481 agcaccattc ccagtttggc ggcttcagtc gttccaactg tactcggtgc agcaaactga
    15541 ttttgggtaa tagtctgcat gagaaggcct gccatactca tactagaggc agtcaggaga
    15601 atactgatag ttcttgggag acgggactct tgaaagagga gccaggtctg ctggtcgaaa
    15661 tcaaatagct ttccccatga aaaatcactg gtcccaatgc taatagagag aaagactagg
    15721 agtagaagta agccaattaa ataatgagaa agtttcatac cccgtccttt catgtagatt
    15781 tggtatcgaa agatacctgc ggatataaat gtaacattat ttttctaatc tgtcaataaa
    15841 atttctgaca atttaataaa tacaacaagg agagagcaac aagactttct cctttgttat
    15901 cctattctaa aatgttttta ccttaatctg ataaaataat atcttcgagg gagtagctag
    15961 ccgtccaatc aagatattgt ttagcttttg aagcatctgc taggacactg gctgggtcac
    16021 tagcacgtcg agcaacaatc tcgtgtggga ttttttaatt tagtaattct tcagcagttt
    16081 taaagatttc tttgatagta tagccttttt tagttcctaa gttaaagatt tgagaagaac
    16141 tgtcttcttg aaataggtag ttcattcctt taacatgagc ctatgcaagg tccaagacat
    16201 aaatgtaatc tcgaatacat gaaccgtcac gtgtatcgta gtcatctcca aatattttta
    16261 agctatcatg ttgtcccaat gcggtcttgt tgatatttgg aatgatgtga gttggatttt
    16321 tcacacgcag accgtttgaa gcatccattt cagccccagc aacattaaag taacggaaaa
    16381 taacatattt ccagtcgtag cgattggcca tccagtaaat cattcgttcg cccatcagtt
    16441 ttgtctctgc ataagggttg acagggtcga gcagggtatc ttcagtcacc ggcttgtcaa
    16501 tacagttatt tccatagaga gaagcagtcg aagagaacat gattttttga atgccaactt
    16561 cagataagac tttgagaact tggtccatac cagcaacgtt ggcagtgaag tatttacttg
    16621 gattttcaat actttcgctc acaacaattt cacctgcaca atggaggaca gcatcaatct
    16681 gattttcttc taagtaagcc tttaaggcgc tagcatcata aacatccagt tctttaaagc
    16741 tagcacgact atccacagcc gctcgatttc ctgtggagag attatcgagg acatgcacct
    16801 gatagccagc atttaaaaga gctttaacgg tatgggagcc aatgtagcta gtcccacctg
    16861 taacaagaat tgttttggtc atgatttttt ccttttctag ttttgtacta tagtaaattg
    16921 aaataagata tgaacaactc tattag
//
LOCUS       DAVKQB010000047        16801 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKQB010000047 DAVKQB010000000
VERSION     DAVKQB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16801
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.047"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..1133
                     /locus_tag="VW528_001636"
                     /pseudo
     CDS             1..1133
                     /locus_tag="VW528_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1321..2883)
                     /gene="guaA"
                     /locus_tag="VW528_001637"
     CDS             complement(1321..2883)
                     /gene="guaA"
                     /locus_tag="VW528_001637"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9912436.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3025..3723
                     /locus_tag="VW528_001638"
     CDS             3025..3723
                     /locus_tag="VW528_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9912437.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERHVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3766..4662)
                     /locus_tag="VW528_001639"
     CDS             complement(3766..4662)
                     /locus_tag="VW528_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9912438.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQTDIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(4671..5606)
                     /locus_tag="VW528_001640"
     CDS             complement(4671..5606)
                     /locus_tag="VW528_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9912439.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLSQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5603..6328)
                     /locus_tag="VW528_001641"
     CDS             complement(5603..6328)
                     /locus_tag="VW528_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU9912440.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(6412..7047)
                     /locus_tag="VW528_001642"
     CDS             complement(6412..7047)
                     /locus_tag="VW528_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9912441.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(7049..7876)
                     /locus_tag="VW528_001643"
     CDS             complement(7049..7876)
                     /locus_tag="VW528_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9912442.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKVRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDIIQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(7898..8233)
                     /locus_tag="VW528_001644"
     CDS             complement(7898..8233)
                     /locus_tag="VW528_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEU9912443.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(8234..8950)
                     /locus_tag="VW528_001645"
     CDS             complement(8234..8950)
                     /locus_tag="VW528_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU9912444.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWFKQADLAIG
                     DFEETINKDHYLAGYPLFNAPAEVMDAIKEAGYHVLDLAHNHILDSQIEGVISTADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNGIKVALLAYSYGFNGIEQYISQEDYNRYLSD
                     LNEDKMKAEIERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHL
                     YVVEPSETIEKDGDKKLIVY"
     gene            complement(9093..9284)
                     /locus_tag="VW528_001646"
     CDS             complement(9093..9284)
                     /locus_tag="VW528_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912445.1"
                     /translation="MKEILICDIIKRKGLHKKVGRMKMQRRQNRNQGGLAFRFMKGLV
                     NFLGVIASGAIRDLWRYSC"
     gene            9473..10084
                     /locus_tag="VW528_001647"
     CDS             9473..10084
                     /locus_tag="VW528_001647"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9912446.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            10129..10857
                     /gene="rlmB"
                     /locus_tag="VW528_001648"
     CDS             10129..10857
                     /gene="rlmB"
                     /locus_tag="VW528_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9912447.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKNLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENSLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(10896..11807)
                     /gene="trxB"
                     /locus_tag="VW528_001649"
     CDS             complement(10896..11807)
                     /gene="trxB"
                     /locus_tag="VW528_001649"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9912448.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVRKIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(11873..12097)
                     /locus_tag="VW528_001650"
     CDS             complement(11873..12097)
                     /locus_tag="VW528_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9912449.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(12175..12918)
                     /locus_tag="VW528_001651"
     CDS             complement(12175..12918)
                     /locus_tag="VW528_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9912450.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(12918..13718)
                     /locus_tag="VW528_001652"
     CDS             complement(12918..13718)
                     /locus_tag="VW528_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9912451.1"
                     /translation="MNVTTILASDWYQNLMQLIPNGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(13876..14232)
                     /locus_tag="VW528_001653"
     CDS             complement(13876..14232)
                     /locus_tag="VW528_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9912452.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(14226..14756)
                     /locus_tag="VW528_001654"
     CDS             complement(14226..14756)
                     /locus_tag="VW528_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9912453.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRSNRRRSTC"
     gene            complement(14756..15250)
                     /locus_tag="VW528_001655"
     CDS             complement(14756..15250)
                     /locus_tag="VW528_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9912454.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            15385..15831
                     /locus_tag="VW528_001656"
     CDS             15385..15831
                     /locus_tag="VW528_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9912455.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            15828..16475
                     /locus_tag="VW528_001657"
     CDS             15828..16475
                     /locus_tag="VW528_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9912456.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILTASVLQYIAIVYYM"
BASE COUNT         5058 a         3789 c         2972 g         4982 t
ORIGIN      
        1 tctaaaattc cttatcttga aacgactggt atgccttcta gaattctcct tagaaaacgc
       61 cgtttcaagt gctatcactg ttcaaaaatg atggttgctg agactcccct ggtaaagaag
      121 aatcaccaaa tccctcgtat catcaaccaa aaaattgctc aaaagttaat tgaaaagatt
      181 tctatgactg atattgccca tcagctttcc atctcaactt caactgttat tcgtaagctc
      241 aatgattttc actttaaaca tgatttttcg cgtcttcctg agattatgtc ttgggatgag
      301 tatgctttta caaagggaaa gatgagcttc attgcgcaag attttgaaaa gctcgatatc
      361 atcactgttc ttgagggtag aacacaagct atcattcgaa atcactttct tcgctacgat
      421 agagttgtcc gatgtcgcgt caaaattatt actatggata tgtttagccc ttactatgac
      481 ttagctagac agcttcgctt tcaaatttct aggctcaggc tgaaacagtc tcccagactg
      541 tttcactccc gaatgctaaa atcgttcttg atcgctttca cattgtgcaa catcttagcc
      601 gtgctatgag tcgtgtgcat gttcaaatca tgaatcagtt tcatcgaaaa tcccatgaat
      661 acaaggctat caagcgctac tggaagctca tacaacagga tagccgtaaa ctgagtgata
      721 agcgttttta tcgtcctact tttcgtatgc atttaaccaa taaagagatt ttagacaagc
      781 ttttgagcta ttcggaagac ttgaaacacc actatcagct ctatcagctc ttgctttttc
      841 acttccagaa taaggaacca gagaaatttt tcggacttat tgaggacaat ctgaagcagg
      901 ttcatcctat ttttcagact gtctttaaaa ccttcctcaa agataaagaa aagattatca
      961 acgcccttca actaccctat tcaaacgcca aattggaagc gaccaataat ctcatcaaac
     1021 ttatcaagca caatgccttt ggttttcgga actttgaaaa cttcaaaaaa cggattttta
     1081 tcgctctgaa tatcaaaaaa gaaaggacga aatttgtcct ttctcgatct tagcttttct
     1141 tcaacccact acagttgaca aagagtctga aaactacggt ctaatttttt gtccaaatgt
     1201 aaaccacttg gaactattat tcaatttatt ctttttagca aatgttcccg ttttaatccc
     1261 agttacagtt tttaatcctt tgtaaccgtt gattttacaa agcttttaat ttttttgcga
     1321 ttactcccac tcaactgttg caggtggttt acttgtaata tcgtagacga tacggttaac
     1381 atgatccact tcatttacga tacgtactga gattttttga agtacttccc atggaatttt
     1441 ggcaaaatca gcagtcatac catcgataga agtgatagca cggattgcaa tcgtgtagtc
     1501 atacgtacga ccgtcaccca taacaccgac tgaacgaacg cctgtgttaa cagtgaagta
     1561 ttgccaaata tcgcggtcaa gtccagcttt agcgatttct tcacgaagaa tagcgtctga
     1621 ttcacgaacg gtttcaagtt tctcttcagt gatttcaccc atgacacgga tagcaagtcc
     1681 tggtcctggg aatggttggc gccatacgat atggtctggc ataccaagct ctgtaccaag
     1741 agcacgaact tcatccttgt aaagagtatt gagtggttca atcaattcaa actgcatatc
     1801 ttctggaaga ccacccacgt tgtggtgtga cttgatagtt tgagctgtat ccgtaccaga
     1861 ctcgataaca tctgtatata aagtaccttg agcaaggaat ttcacatctt tgagcttgct
     1921 tgcttcgtca tcgaatacat agacaaactc gttaccgatg attttacgtt tttgttcagg
     1981 gtcagaaacg ccagcaagtt tgtcaaggaa acgtttagca gcgtctgctt tgacgatatt
     2041 caaaccaaac ttaccaccga gcatgtccat aacttgatca gcttcgcctt tacgaagaag
     2101 accgtggtcc acgaagatac agatcaattg atcgccaatc gctttttgga gaagaacccc
     2161 aacgacagat gagtcaacac cacctgatag accaagaagg acacgtttat caccgacggt
     2221 ttcacgaatt tttttgatct gcatgtcaat gaaattatcc attgaccagt cacctttagc
     2281 cttacaaatg ttaagggcaa agttacgaag gatatcattt ccgtatacag aatgacgaac
     2341 ttctgggtgg aattggatac cgtaaatgtg tttatctggg ttttcgatgg ctgcgtatgg
     2401 gcagtcagct gatgtacctg tacgaacaaa gtcagcagga atctcagtaa ccgcatcacc
     2461 atggctcatc aaaacagtct gttcatcagg tgttgattca aaaagcgctg atggtgtgtg
     2521 agttagggtt gattgaccgt attcacgatt tccagcatca cctgcaggaa caacttttcc
     2581 tccaagttta tgggtcaata actgcatacc ataacagatt cccaaaattg gaattccgag
     2641 ttcgaagatt tctgggtcaa tatcaaatga accatcttca tatacagaat ttggaccacc
     2701 tgatagaata attcctacag gattgacttc acgaacttca gcagctgaaa ttttatggct
     2761 ttttagttct gaaaaaacac caatctcacg gatacggcgt gaaatcagct ggttgtactg
     2821 gctaccatag tccaatacga tgattttttc tacatcttgc aaatcagttg aaatgttgct
     2881 catctttttc ctttctcaaa agaaatatct tttctcaata ttctatcaca aataagggcg
     2941 aattaccaac taaacaaggc gaagtttgac tttttctagt ttattggggt acaatagaga
     3001 aaagatagaa atgaagtgaa aaatatgcta cctgcttata tgaaaatcca tgatcagata
     3061 aaaaaggata ttgacgagca ccgttgggct attggtgaga ggcttcccag tgaaagagat
     3121 ttagctgagc agtttgcggt cagtcgcatg accctccgcc aagccgtatc tctattagtc
     3181 gaagaaggcg tcttagagcg ccatgtagga agcggcacct ttgtttccag tactcgagta
     3241 caagaaaaga tgcgagggac aaccagtttt actgaaattg tcaaatccca aggtaaagtt
     3301 ccctctagcc agctcatttc ctacaaaaaa accattccca atgagcaaga agttgccaag
     3361 ctaggaattt ttccaacgga caatattatc cgaatggagc gggtccgcta tgccgaccaa
     3421 gttcccctag tttatgaagt tgcttctatt cctgaaaaat tcattaagga ctttaaaaaa
     3481 gaagaaatca ccagtcattt cttccaaacc ttgcaaaaac atggctatcg tatcggcaaa
     3541 tctcaacaga ccatctatgc tcgccttgct aaagaaaaga ttgcccacta tttggaagtt
     3601 gaaaaaggac atgctattct tggattgacc caggtttcct acctagaaga tggtactgct
     3661 tttgaatacg taaaaagtca atatgtaggc gaacgctttg aattttatct tgaaaataat
     3721 tagattacca cgcaaaaact ctctgtcacg gacaaagagt cttttttatt tttctaagaa
     3781 taaatctttc aaagaaatca aagttacagc aaacaatatc attgccatac caagaaaatc
     3841 aatgggataa aattgttcat tcattattaa gaaggcaaag aaaatcgccg atattggctc
     3901 aattgaagcc aacaagcttg acttgaccgg tcctatcaga ctggctcctt taaggaaagc
     3961 tgtataggca aagacagtcc cgataaggat aatgcctgca aacgcaagga gaaaatcaag
     4021 actagtcggg atatcggtct gtagaacccc tgtaaaagga agggcgacca aacctgctat
     4081 gaccattccc acaccaatga ccaagctgct cccccacttt ttaatcaagg ctatgggtaa
     4141 aatgatatac agagcataag tcaaggcaga aaagagaccc cagaacagac cagcaggtgt
     4201 catggataac tggtccaact gcccatgtgt tgcgatcagg aaggttcctc cgatggcgaa
     4261 tatgatggaa actatctctc ccagtgtcgg tgccacccta tccttgatac agctataaat
     4321 taaaattccg acaggacaaa catactgaag caccgtcgct gttcccgcat tggtctcctg
     4381 aatagcagac agataggcga attggttgag aaaaagacca atcagagcaa aaataagaag
     4441 agacagcaaa ctctttctat cctttaaaaa gaccagtatt ttatcctttg cagtagcata
     4501 agccaagagc atgagaattc caccagcgat taaaagacgc aagttggtca agaccagagc
     4561 cgaaattccg tgtgccatta ggtattggcc actcgttcct gacaaccccc aagcaatacc
     4621 agccacaact gttagtaaag tcccttttaa actatttgac atgtctctcc ttactcttct
     4681 ttgcgaatca agttcatcac ttgattgcgg tctaatatcc attgagctct ctcgtccttt
     4741 tcataggtac gattcgatag gaaaatagtg gcttcttgct tctgacgatt ccacatgata
     4801 aaggtacctg tatagcccgt atggtctagc caatctcctt ccaaattcca tgctaaagaa
     4861 cgttccttgt catccaaagg agaaaaattt tgacttaagt ctcttgcaaa atcatctgct
     4921 aaatagtgtt ctaaaaagat ttgtaaatcc tttatagtcg aaaataaacc agcgctccca
     4981 gcatgtctac ccaggagacg agccttggga tcatgcacta tgcctgcctc tacacctcta
     5041 actgttggaa cagcaagctc aactggccca aacttggttt ccgtcattcc ccaaggtttc
     5101 cagacttgat cctttaaaat cacatccaaa tcttgattaa aaattctttc caaaataaag
     5161 cccaacagca aaaaatggac atccgaataa agaaaggctg gctgacttcg tctgttgaga
     5221 tgaaacatcg cttcctttaa ttcaggggct gttaaaagat cacgattagg aataaaagga
     5281 tcaaggtctg ttgcatgagt caagagctgg cgaatagtga tgtctggata atcactctca
     5341 ggtaaaaaat ctattaccag tctatcaata tctaattgac ctatttccca caagaaggta
     5401 caaactgtgc caaccccaac aaccttgctg acactagcta ggtcataaac tagtcctgcc
     5461 tcagtctgca agccatgctc tgggtcactc tggcctaaat agaactctgt ccattgattg
     5521 tccttaaaat acgcaaaaga ggctccggga taaattcctg cctcgatttg ttcttctatt
     5581 tttttaataa tcttggtcca cttcatactt cttcaaacca caattcaaca ttatttctat
     5641 ctttacaaag gaagaatttt tcagacttag gaataaaata ttcagtagac tcaaacttct
     5701 ggcgaagact tgctatgtct aattcattga ccaagaactt gagcatagat aagtcccagg
     5761 taaccgtatt gtccacagtc aaatcctgcc cctgagctgg aataaaatca agggatgccc
     5821 caatttcaga tgattccaag aaactttcga tatcagttgg gagatgtaat tccatagaaa
     5881 tctcaaattt acttaaagaa attgatgcca aatctgtttg aaattcaggc ttttctccta
     5941 cttctactag acttgctctg tcatcttccg catgaatcaa aatcaaatca tcttctggtg
     6001 agaaaatttc aaaagcgtag ccattttgac ctttatataa tcgatgaatc gaatctgttt
     6061 tagataagat tccttcaatt tctaagggat tttccacctt gacaatcaat ctagctagtt
     6121 tttttcttcc ctctacctta cgagtacgca tactgggagc ttcttctaaa accagctttt
     6181 caagacccgt ttggtcacct agtgacagaa aggccgattc ttctaacaag gccttcattc
     6241 caagggtttc aatataaaat gtttcattta attttctatt attagctttt aaagtaggaa
     6301 taatccgtac aatctgattt acattcataa attcctccgt cactttttat tttaaaggat
     6361 tttagaattt tttacaagat attatgctca aatttaacat tatttagcta tttagtaaag
     6421 atagtctttc aaggattttg acaaagcctg ataaccagca atactgaggt gcagtccatc
     6481 agttgtatat tcttttttga gttggcctgc ttggtctgtc aaacaatcaa atactggcac
     6541 aaattccacc tgcatatagg cagatgcaag ctcttgatag gcttgattcc agttctgaat
     6601 tttttcattc gagcggatat agactgcctg ctggtactcc tctccctcat tgacaggcaa
     6661 aatggaaagc aatttaatct ctgtcaatgg ataatcgcga gcaacggatt gaatgatagc
     6721 ttcgagatta ttgagagcct cattcacagg aacatccttt ccgatatcat ttgtcccaat
     6781 cagaagaaaa attttatcta ctgctccacc atatagatga gcatcaagat tctctagtaa
     6841 cagtcctgtc tgatagccac gaattcctcg attgacaatc gtctttgaag tcccaaatag
     6901 ctcctgtaga ggataatact cgacaatgga atccccaata aaaagaatgt ttggttctac
     6961 aacagaaatg tgatttaggt gacgatactt agtttgaatt ttttcttgtt cctttaggag
     7021 ccaattttct aataactgta ctgccacttc attctccttt ttctaaccag ttttccaaga
     7081 cttgataaac tcctccttgg ctgttggctg gtgctagagc agtcgccaca gctttggctt
     7141 tctcatcagc attttccatc gcataggcaa ttccagccat ttcaagcatt tcaacatcat
     7201 tttcactatc accaaaagcc atgatttctt gggatttcaa gtcccagcgc ttgagtaatt
     7261 cctccaagcc ccatgcttta tgaatcccag cttggatgat atcaatgcaa ccatagccac
     7321 tggatacagc tcggacacgg ccatcaaaga gagcattgat ttcttccaaa accgaactca
     7381 aacgttcctc accaacaacc atgctcatct tgagcacacc accaaagagg tcagatgtta
     7441 attcatccac aaattgcatc cgttggtaga atttttcaat catttctgga gtcataaaac
     7501 tttcaagatc tgtaaaaatc gtaccttcct tgacaaaatc acccttcatc cccgttacaa
     7561 caaactggtc ctgacacgct cgaaccttga aatgagtcaa agccttgttg acaatggcgt
     7621 catcccatgt ctgagcctga atcaattcat tgttttcaaa aatacgagcg ccattagcaa
     7681 caaccagaac cactcgatcc accaagggac tcagtagttg tctcatgcgg tgaatttcat
     7741 tgcccgtcgc aatgacaaaa cgaatgcccc tttgatccaa ctgatctaaa atcttttcca
     7801 atcgtgggag atcaagctga cctctagcat ccagcaaggt cccatccata tctgtcgcaa
     7861 ttaccttaat catcatttac tttcctctgg attcaagcta ataccacttc aatcccacat
     7921 gttcattcat ttctttatag gctgtatcaa ttcgttcctt agtcgcttca tctaactggt
     7981 cacgatgact gccatcctct ataaaatctt ccaaaatata agtttggtaa tgatacaagg
     8041 gatatccttc tggtgaaaag gttccctttg gtgttcgatt gacccaagta ggatgagctt
     8101 tagctgttcc gatagttgtt tttccatcct tcttcttgat ggtgacatcc atgagaacac
     8161 cacgttcagt ccacttagca ttctcttcat ctcccataga ttcaattcgt tgattggaaa
     8221 tgaagttccc catttaatag acaatgagct tcttatctcc atctttttca atcgtttcag
     8281 atggttcaac aacgtaaaga tgccctccaa agataatatc cgctccccaa tcgatcatct
     8341 tgtgataaag agctttttgt tcttcagttg gttccaatcg atactcaaca cccatctgag
     8401 gcatgataat ggtgatatct gcttccttct ctgcccgttc aatttcagcc ttcatcttat
     8461 cttcgtttaa atctgaaaga taacgattat agtcttcctg agaaatatac tgctcaattc
     8521 cattgaaacc ataggaatag gctaacaatg caaccttgat accattcact tccttaatga
     8581 ccagcggagc ctgatcacgt ggttcgtgcg tataaactcc gattggagtg attccagctt
     8641 tctcaataat atcggccgtt gaaataactc cctcaatttg cgaatccaaa atatgattat
     8701 gagctaaatc cagcacatga taacctgcct ccttaatagc atccataact tcagcaggag
     8761 cattaaagag aggataaccc gctaaataat gatccttatt aatggtttct tcaaaatcac
     8821 caatagctaa atctgcttgc ttgaaccaag gagtcacata ctcaaaattt tcatgaaagt
     8881 cataggtacc gtcttctttt ttagctgaaa agaaaagtcc atcgtggtag agcaaatccc
     8941 cgtgggccat aattctggca gttttttcct cttcctactc ctgagacttt tgcttagttc
     9001 cctcttgaga aatagtatct cgtttttgac tagtcaagag attccttgga agctttcaaa
     9061 caataagacc aagcccattg ataaaccaac tgctagcaag agtatcgcca caaatccctt
     9121 attgctccac ttgcgataac tcctaaaaag tttaccaagc ccttcataaa acgaaaagct
     9181 aaaccaccct gatttcgatt ttgtcttctt tgcatcttca ttctccctac tttcttatgc
     9241 aagccttttc tttttattat atcacagata agtatttctt tcacaattga attgaacttc
     9301 ccatctattt tctataaatc ctaaatgcca taatgctttc aattcttgtc attttgtgat
     9361 atcatgtaga ggtaatgaaa ggaaatgaac taatccacag tggcttattc caagtatacc
     9421 acttgggctt tggcagtagt taactgcact aaacataata taaggagaga aaatgtctgc
     9481 aatagaacgt attacaaaag ctgctcactt aattgatatg aacgatatta tccgtgaagg
     9541 gaatcctact ctacgcgcga ttgctgagga agtcactttc cccctatctg accaggaaat
     9601 catcctaggc gaaaagatga tgcaattcct taaacattcc caagatcctg tcatggctga
     9661 aaaaatggga ctccgcggtg gtgttggact ggctgctccc cagttagata tctcaaaacg
     9721 cattatcgct gttttggtac ctaatattgt tgaagaaggc gaaactccac aggaagccta
     9781 cgatttggaa ggcattatgt acaatccaaa aatcgtctct cactctgttc aagatgctgc
     9841 tcttggcgaa ggagaaggtt gcctgtctgt tgaccgtaac gtgcctggct atgttgttcg
     9901 ccatgcccgc gttactgttg actactttga caaagatgga gaaaaacacc gtatcaaact
     9961 caaaggctac aactccattg ttgttcagca tgaaattgac cacattaacg gtatcatgtt
    10021 ttacgatcgc atcaatgaaa aagacccatt tgcagttaaa gatggtttac tgattcttga
    10081 ataaagaaaa tcccgttgca agacggggtt ttgtgttata atagaggcat gaaaacaaat
    10141 gatattgtct atggtgtcca cgccgttacc gaagccctcc ttgcaaatac aggaaacaaa
    10201 ctctacctcc aagaagatct ccgaggtaag aatgttgaga aagtcaagga actagctaca
    10261 gaaaagaagg tgtccatttc ttggacatca aaaaaaaatc tctctgagat gactgaaggt
    10321 gctgttcatc aaggttttgt tctacgagtg tctgaatttg cctatagcga gctagattac
    10381 atccttgcaa aaacacgcca agaagaaaat tcacttctat tgattctaga tggtctaacc
    10441 gatccccata atctgggttc tatcttgcga acagccgatg cgaccaatgt ttcaggtgtc
    10501 atcattccca agcaccgtgc tgtcggagta actcctgtcg ttgccaaaac agccacaggt
    10561 gctattgaac acgttccaat tgcccgagta accaacctca gtcaaacctt agataaactc
    10621 aaggatgaag gtttctggac ctttggaacg gatatgaacg gtactccttg ccacaagtgg
    10681 aatacaaaag ggaaaatcgc cctcatcatt ggaaatgaag gaaaaggtat ctctagcaac
    10741 ataaaaaaac aggtcgatga aatgattacc attccgatga atggacatgt tcaaagcctt
    10801 aatgccagtg ttgctgcggc cattctcatg tacgaagttt tccgaaatag actataaaaa
    10861 agtttccagt catctgattg gaaacttttt tatgattaac tatgttctgt aatgaattta
    10921 taggcttctt gaccagcgat agctccatct ccaactgctg ttgttacttg gcgaaggtct
    10981 ttcaagcgaa catctccaac tgcaaagata ccgtcgactg cagtcttcat gtggctatct
    11041 gtcacaatcc agcctgcctg atcttggata ttcaattctt taacaaaatc gctaagaggg
    11101 tccaaaccaa catagataaa gacaccaccg aaggcttgct ctgtcacttg acctgttttc
    11161 acattttcaa aaacgacaga ttctactctg ttttcaccct tgattttcct tactacagaa
    11221 tcccatataa agctgatttt ctcattggca aaggcgcgat cttgtaaaac cttttgggca
    11281 cgaagttggt cacgacggtg aacaatggta acagtcttag caaaacgagt caagaagagg
    11341 gcttcttcaa cagctgaatc tccaccacca actaccaata aatcttggtc acggaagaaa
    11401 gcaccatcac acacagcaca gtaagaaaca ccacgactgt tcagttcttc ttctccaggc
    11461 actcccaaag gacggtgttt agaaccagtt gctacgataa ctgtacgtgt ttcatatgtt
    11521 tggtcatcag tcatcacttt cttaaaatca ccatggtctt cgacattttc aacataacca
    11581 taaatgtgct caacaccaag attttcaagt ggttcaaaca tcttttcagc caattcaggt
    11641 ccactaatat tagcgtatcc tgggtaattt tcgatatcag atgtattatt catctgacca
    11701 cctggcagac caccttcaat caaagctact tttagattgc ttcgagcagc atacaaggcc
    11761 gcagtcatcc ctgcaggtcc agcaccgata ataatagtat cgtacatata gattccttct
    11821 ttcttgttgt aactatcttt attctaactc tttcttgtca atcacgcaag gattatgcct
    11881 tgaaaacaag aattaaactc ataaccgcaa aggataatac tgggacaacc aagactccaa
    11941 tcataatcat atcatagaga tgggcttggc gagccttggc tgtcttatca actcccgaca
    12001 tggctctcag accaatccaa atccctaaaa aaatcagaac aaggatggtg gtcaagatca
    12061 aactctcgaa atataaagaa aatagttgca gtagcatgat ttctctcatt tctatctttt
    12121 ttaaagagta aactcagcta gtccaactaa ctgagttttc ctttatctat tatatcaaat
    12181 ataagtccgt ttgtaactag cgaagaattc ttttgtccgc tcttctttag gggtgtggat
    12241 aatctcatcc ggagttccag actcgatgat tttcccctta tctaagaaga gaatcttatc
    12301 cgcaacttgg gctacaaagg acatgtcatg actgaccaaa atcatggtct gacctgactt
    12361 agcagcatct gcaatagact tttctacttc accgaccaat tctgggtcaa gggctgaagt
    12421 tggttcgtct aagagcaaaa catctggttt catagcaagc gcacgcgcta gggcaacccg
    12481 ttgcttctgt ccacctgata aatggcgagg ataatggttt tcacggtccg aaagcccaac
    12541 cttagccaac tcttccttgg caatcttagt cgcttcttgg tcagataatt tcttgacaac
    12601 aaccaagcct tctttcacat tatcaagtgc tgttcggcgt tcaaacaaat taaactgttg
    12661 gaaaaccata gacaacttac ggcgtagggc aaggatttct tcttgagtga ttttagaaaa
    12721 atcaactgaa aaaccatcaa tctgaataga gccactgtca ggtgtttcaa gataattgag
    12781 actgcgaaga aaggttgatt ttccagctcc tgaagaacca atcaaggcta caacttcccc
    12841 tttttgaata tccaagtcca gatgatccaa gacagtctgt cctgaaaagg atttgcttaa
    12901 attcgaaatc ttaatcatta acgaaggtct cctttcacat ctgtttgcac tgtatcaggt
    12961 gcagaaatag ccatttttct ctcgatgaaa cgaccgagac tttcaattcc gatattgact
    13021 acccaataaa caagggcaac ggagatgaag cgttcaaaat agcgataatc agctccacct
    13081 agaatctgag cttgggcaaa gacttccaca acacccgcac taaaagctag agatgttccc
    13141 ttggtcaaac cgatgaggga attaatcaag gttggagtag ctaccaccgc tgcattagga
    13201 ataatcactc gtcgataaac ttgcgctcgg gtcataccca gactgcgtgc cgcctcaatc
    13261 tcaccaggat taactgagag aatggctgca cgaatggttt cactagcata agctgcctca
    13321 ttaaaggcaa aggcgacaat cgcaaaagct gcagctggaa tcgcattgat attgagacca
    13381 gttccccatt gctgattgag ggctttcaaa gccaaaggga ttccgtagta ggtcaacatg
    13441 agttgcacca aaatcggtgt cccttttaag aaactaacaa agaaggcctg caagggatat
    13501 aaaatcttga cacgattgat cttcacaatg gcaaaaagaa gcgccaaaac caagccaaaa
    13561 agggcaccac caattgtcaa cataattgtt gttggaagtt gttggacaat tctagggatt
    13621 ccatcaaaga ccgaacgtag gctaaacagc ttgccattcg gaatcaattg catcaagttt
    13681 tggtaccaat ctgatgctaa aatcgttgta acattcataa aaacatcctc ttctgataat
    13741 gattcattct accatgcttc tgccgtcaat gaaattattt gctacggtca gatacagact
    13801 ttgtctacct actagtttat catactttga aacagggaag ttatatattt tatctatcaa
    13861 agagatagat aaaaactatt tcagtcccag ttcttcataa gattttcgat aaccgatttg
    13921 cttaacggtt ccattttcta ctaaaatggg ccgttttaac aacataccgt cacttgctag
    13981 caactcagcc gcttcttggt ttgacaaact tcctacctta tcttttagcc ctaattcacg
    14041 gtatttgata ccactggtgt tgaaaaattg cttcaattca aatcctgagg tttctagcca
    14101 attcaaaatg acttcttggc taggtgtttc ttccacgata tggacggctt tatagtccac
    14161 acctaattga tttaattctt gttttgcttt tttacaagtt gaacatttgg ggtattcgat
    14221 aaattctaac atgtgcttct ccttctattg cttctatctt taaaatctac tccttcatgc
    14281 tccaagagcc aagctttctt ttccactcct gcagcataac ctgtcagacg cttgcctgct
    14341 cccaacacac gatgacaagg tactaggata gaccaaggat tgcgtcccac tgctccacca
    14401 attgcttgag cagaagccac ttgcaggtct tgagcaattt gtccataggt cactgtctga
    14461 ccataaggaa tgccctgtaa atagtcccaa actctctttt caaaattcgt tccgattggc
    14521 gccaagagca agttggataa atcctgaggc ttgcctttaa agtaatcatc taagcaagca
    14581 ataactgggt ctaaaatagg atgactaaca acttcttcta tcgtttcatc tcctagtccc
    14641 ctctcaaaat gcttctgctc ctgaacccaa attccataca aataatggtc atcagctaca
    14701 agtgatagaa taccaattgg tgaggagtag agtattttta catacttctt ttgcattatt
    14761 tctttaattt ttgataggcc aagcgttcgc catctactgg caccttacca acttgtttaa
    14821 aaccaagttt ttcaaaaata tgttgcataa ccttgttttc agcatgcgta tctgagcgaa
    14881 aatcaagata atcaaaacct tcaatcaagc cctctaagaa ggtttgagca actccttttc
    14941 cctgcacatc tgctgccaca gcaatacggt gaaagactag atactctgac tctccagctt
    15001 gccagtttcc ctcataaata gcttcatagg cctcctctgg actcttggtc acagcagcat
    15061 aggctagcag ttctccctct tccaaggcta cgtaggcttg ccctgagatg atatcatcga
    15121 taataacatc tgcatttgga tagccatttt gccactggtc actaccagca tctgctaaac
    15181 attttttggc atcctccatc acctgcatga tggcatctac ttcatttgga aaagctaaac
    15241 gaatttccat cttttttcct catttctgac tagtttctcc catcatagca taatttaagt
    15301 atttctacaa gcagttaaaa cttgactttg tgtttttgtt attttataat ctagttatca
    15361 aaactatata aaaaggagtt gaaaatgaaa actacctttt cctacccaaa atgggcagaa
    15421 attccaaaca ttgacctcta tctggaccag gttttgctct atgtcaatca ggtctgcgcc
    15481 cctatctctc ctaataaaga caagggccta acagcatcta tggtcaacaa ttatgtgaaa
    15541 aatggttacc tgacaaagcc tgacaaaaaa aaataccaac gccaacagat tgcccgtttg
    15601 attgctatca caaccctcaa gtctgtattt tctattcaag aaatagccca gacacttaat
    15661 actctacaaa ctcaagcaag ttcagaccaa ctctacgatg cttttgtgga ctacatgaac
    15721 caaggaattg acccagctaa ccctattatc caaaccagct gccaaaccgt taaactctat
    15781 catcaaactc tagacttaat cgatcatact caagaggagg taatccaatg aacactagtc
    15841 ttaaactcag caaacaactc agttttggag aggagattgc taatagcgtg acccatgctg
    15901 tgggtgcagt catcatgctt atcttgctgc ctatttcatc catctatagt tatgaagcac
    15961 acggattttt atcctctatc ggcgtttcca ttttcgtcat cagtctcttt ctcatgttcc
    16021 tatcatccac catttatcac tctatggcct atggttcgac ccacaaatat gttttgcgaa
    16081 tcattgacca ttctatgatt tacgttgcca ttgccggctc atacacgccc gttgtcttga
    16141 ccttgatgaa taactggttt ggctatctga ttattgtcat ccaatgggga acgaccatct
    16201 ttggtattct ctataaaatc tttgctaaaa aggtcaatga gaaatttagc cttgctcttt
    16261 acctgattat gggctggttg gttctggcta tcattcctgc cattatcagt caaacgacac
    16321 ccgttttctg gagtctcatg gtaactggcg gactctgtta tacagttgga gctggatttt
    16381 atgccaagaa aaaaccttat ttccacatga tttggcatct ctttatccta actgcgtccg
    16441 tacttcaata catcgctatt gtttattaca tgtaaaactc tcagtaggca gagcctgctt
    16501 agagttttac aaacgaattt cccaaagagg aagcaatatg aaagcagtca ttgcttgtgt
    16561 tcataaaatt ctcagaatca tttacaaact actctccacc aagtagactt atcaaaaaga
    16621 aaaggcgcta ggactgaaga aacagttcta acgcccaaat taaaaaaatt tcaactacag
    16681 tatagcacag gaaatgattt tttgcgcttt tttactgttt tttcaaataa aaaaagacaa
    16741 agttttatcc ttgtctttta gttttagtat tgctttggtt tcaatgtcaa taagtgtgtt
    16801 c
//
LOCUS       DAVKQB010000048        15052 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKQB010000048 DAVKQB010000000
VERSION     DAVKQB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.048"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     regulatory      79..167
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            382..1050
                     /locus_tag="VW528_001658"
     CDS             382..1050
                     /locus_tag="VW528_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9912457.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYNV"
     gene            complement(1200..1286)
                     /locus_tag="VW528_001659"
                     /pseudo
     CDS             complement(1200..1286)
                     /locus_tag="VW528_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            1377..2159
                     /gene="thiM"
                     /locus_tag="VW528_001660"
     CDS             1377..2159
                     /gene="thiM"
                     /locus_tag="VW528_001660"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9912458.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEIAEKRPNGHLPGTFKAEFINALYEITDEDVKEFKRVK"
     gene            2161..2790
                     /locus_tag="VW528_001661"
     CDS             2161..2790
                     /locus_tag="VW528_001661"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9912459.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGAITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      2999..3087
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3349..3909
                     /locus_tag="VW528_001662"
     CDS             3349..3909
                     /locus_tag="VW528_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9912460.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            3910..5295
                     /locus_tag="VW528_001663"
     CDS             3910..5295
                     /locus_tag="VW528_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912461.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            5297..5947
                     /locus_tag="VW528_001664"
     CDS             5297..5947
                     /locus_tag="VW528_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9912462.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            5958..6650
                     /gene="tenA"
                     /locus_tag="VW528_001665"
     CDS             5958..6650
                     /gene="tenA"
                     /locus_tag="VW528_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9912463.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            6655..7179
                     /gene="thiW"
                     /locus_tag="VW528_001666"
     CDS             6655..7179
                     /gene="thiW"
                     /locus_tag="VW528_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9912464.1"
                     /translation="MRKRQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            7179..7982
                     /locus_tag="VW528_001667"
     CDS             7179..7982
                     /locus_tag="VW528_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9912465.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLNYRPTVIKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSKNQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEAYE"
     gene            7975..8607
                     /locus_tag="VW528_001668"
     CDS             7975..8607
                     /locus_tag="VW528_001668"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9912466.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(8816..9607)
                     /gene="thiD"
                     /locus_tag="VW528_001669"
     CDS             complement(8816..9607)
                     /gene="thiD"
                     /locus_tag="VW528_001669"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9912467.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQAKDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(9691..9789)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9973..10368
                     /locus_tag="VW528_001670"
     CDS             9973..10368
                     /locus_tag="VW528_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9912468.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            10379..10750
                     /locus_tag="VW528_001671"
     CDS             10379..10750
                     /locus_tag="VW528_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9912469.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            10760..13003
                     /locus_tag="VW528_001672"
     CDS             10760..13003
                     /locus_tag="VW528_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9912470.1"
                     /translation="MTEIVKASLENGIQKIRIRSEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            13210..14985
                     /gene="spxB"
                     /locus_tag="VW528_001673"
     CDS             13210..14985
                     /gene="spxB"
                     /locus_tag="VW528_001673"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9912471.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
BASE COUNT         4424 a         2767 c         3329 g         4532 t
ORIGIN      
        1 tatatataaa gatatttttc ggtcaaatat cttgctaggg ctttcatttt ttgctatact
       61 ggttcagtaa ttaaataata gagacatttg gggtgcttta ggctgagata atacccattg
      121 aacctgatac agttaagact ggcgaaggga aatgtgaaca gttttttcgt atggtgaaat
      181 gaacggtcta atcttgtaag tcaactctaa ttcttgattg taattggagt tttttgttta
      241 tataaactgg ataggcttgt ttagttagct ctctgcaaat gctctcgatg tgctttaaaa
      301 ggatcaagtg gaattacgat tttgcttgat atttcttggt gttttattaa ttactaacaa
      361 gtaaagagag gagaagtaaa tatggaaaca caagactatg catttcagcc aggtttgact
      421 gttggagaat tattaaaaag cagtcagaag gattggcagg ctgcaatcaa tcatcgtttt
      481 gttaaggaac tttttgcggg gacaattgag aataaggtct taaaggacta tctgattcaa
      541 gattatcact tctttgatgc cttcttatcc atgctgggtg cctgcgtagc tcacgcagat
      601 aaacttgaat ccaagcttcg ttttgccaag caactaggct ttcttgaagc agatgaagat
      661 ggttatttcc aaaaggcttt taaagagtta aaagtagcag agaatgacta tctagaagtg
      721 actttgcacc ctgtaacaaa agcctttcag gatctaatgt attcggcagt ggcttcatca
      781 gactatgccc atcttttggt tatgctggtc attgcagaag gtctctattt agactggggt
      841 tctaaagatt tggctctacc tgaagtctat attcattcgg aatggatcaa tctccacaga
      901 ggtcctttct ttgcagagtg ggttcaattt ctggttgacg aactcaatcg tgtcggtaaa
      961 aatcgagaag atttgacaga acttcagcaa cgctggaatc aagcagtcgc tttagaatta
     1021 gctttttttg atattggtta caatgtctag agaaggctca tgatatttac aaatttgatg
     1081 aggtaagact cttcgaaaat ctcttcaaac aacgtcagtt tcaccttgcc gtaggtatgg
     1141 ttactgactt cgtcagttct atccacaacc tcaaaacagt gttttgagct gacttcgtca
     1201 gtcttatcta caggttcaaa gcagtgtttt gagcaacctg cggttagctt cctagtttgc
     1261 tctttgattt tcattgagta taaatcattc aatgtaaatg aaaaaaattt cttaacgaaa
     1321 gatagatata aatcgaaatc agtagatcgt ataaagtgaa aaggaaggat ttcaatatga
     1381 caagtttaaa attattaaaa gaaaaagcac cattggtcat ttgtataacc aatgatgtag
     1441 taaaaaattt cacagcaaat ggattagtag cactgggcgc ctcaccagcc atgagtgagt
     1501 ttccagcaga tttagaggat ttgttaaagt atgctggtgg tttattaata aacataggaa
     1561 cattgacaga tgaaaattgg aaattatacc aagctgctct gaaaattgcg gagaaatata
     1621 atgtcccagc agttttagat cctgtagcct gtggagcagg agaatataga aaaaaagtag
     1681 cggatgatct aatcaacaat tataaactag cagcgattag aggaaatgct ggcgagattg
     1741 cctctttagt aggaatagat gtggcatcta aaggagtaga tagtgcgggc gtagataata
     1801 ttgacgaaat tgctctagca gcaaatgaga agttcaatat tccaatagta gtaacaggtg
     1861 aagtggatgc cattgctgtt aatggagaag tggtaacgat tcataatggt agtgctatga
     1921 tgccgaaagt cattgggaca ggatgcttat taggagctgt agtagcaagc tttatcggac
     1981 tagaaaaagg tcaagaattg aaatcattag aaacagcaat gttggtttac aatatcgctg
     2041 gagaaatagc agaaaaacgt cccaatggac atcttcctgg aacatttaaa gctgaattta
     2101 taaatgcctt atacgagatt acagatgaag atgtaaagga attcaaaaga gtgaagtaag
     2161 atgtttcata aagaattact aaaactatat tttatttgtg gaacgactac ttgccaagga
     2221 aaaaatctat atacagtcgt tgaggaagcc ttaaaaggtg ctataacctt atttcaattt
     2281 cgagaaaaag gtgagggtgc tttagaaggc ttagaaaaac ttgagctagc tatccaaatt
     2341 aaagaacttt gtaaaaaata caatgttcca tttatcgtta atgacgatat agatttagca
     2401 atggaaattg acgccgatgg cgtacatgta ggtcaagatg atattggtgt tgatgaaatt
     2461 agaaaattga tgccagataa aataattggt ctttctataa gaaacgaaga agaatttcaa
     2521 caatcaaaag ttgaatatgt agattatgta ggtgttgggc ctgtatttga tacccagtca
     2581 aaagatgatg ctggtggtgc tataggttat gaaggtcttg aattgatgag aaaactattg
     2641 ccacaaatgc ctttagttgc aattggtggg atacagacga aacatattaa agacattata
     2701 aagaccaatg ttgacggcgt ttcaatcatt tcggcaatat cgtatgcaaa aaatatagaa
     2761 aaaactgttc gggaaatgag tgaacaatag atatctatcc ttggattcta agtaagtagg
     2821 attttccttt tttaaaaagc tatgtctaga ttcagcttga atggctaatt catcaaacag
     2881 gtcatttgtt ttttgcaaaa gaaagatctg ctgtctttgt gagtgcaagg ctgtatttcc
     2941 agttcaattt cttgaactag tataaaatgt gtgttataat aatcacataa ttaaataata
     3001 aaggcatttg gggtgcttta ggctgagatg atacccattg aacctgatac agttaagact
     3061 ggcgaaggga aatgtgaaat gtttttttcg tatgtcgaaa agaactgtct aatcttgtaa
     3121 gctgaactct aattcgtatc tgtaattaga gttttttctt ttacgaaact ggatgaagat
     3181 gatatagtag attgaaataa gatgcgaaca aatcaattag aaaattcaaa ttaatttcta
     3241 gaaatatttt agcattcctg tgtactgttc taaattcaat ctgctataca aagtatttag
     3301 gtcggtcttc ttgagtgccc cacttttctt ttaaaaaagg agttttttat gttgaaaaaa
     3361 tggcagttaa aagatgttat cttgcttgct ttcttgtcta tcttttttgg tggggttttc
     3421 gttggttcag gatatgtgta taatattctc agtctactct taacacctct tggtttgcag
     3481 gcctttgcca atgaaatcct cttcggtctc tggtgtatgg ctgcgcccat tgctgccatc
     3541 tttgttccga gagtcggaag tgcaacgatt ggagaagtgc tagctgcgct tgctgaagtc
     3601 ctttatggta gccaatttgg tctaggagct cttttgtctg gcttggttca aggtttggga
     3661 agtgaatttg gttttatcgt aactaagaat cgctatgaaa gttggctctc tctaactgct
     3721 aatagtattg ggattacgct tgttagcttt gtctatgaat acattaagtt aggttactac
     3781 gccttttccc ttccgtttgt cctttcctta cttgtggtac gttttatttc tgtttatttc
     3841 ttctgtacca tcttggttcg tgccattgtc aaactctatc atcagtttgc aactggagga
     3901 aaagcataga tgggtctgga actacgagcg attcagtccc caatcttctc tgagccgttt
     3961 gattttactt ttcatgcgca agcctttacc ttgttagttg ggagcagtgg ttcaggaaaa
     4021 tccagccttt ttcaagtcat tgcccaagtc agttctcttc cctatagtgg tcaagtcctg
     4081 atagatggga gcgaggtcag tcagctttct atcatcgcac gtgtccagaa ggttggcatt
     4141 ctctttcaaa atcccaatca tcaatttacc atggagaact tgtttgagga gctgattttc
     4201 accttggaga atatcggcta tcaccttcag gaaattgatt ctaaaatagc agaggttgtc
     4261 cagcaatgtc gttgcgaggc aatcttacac cgtcccattc atcacctatc aggtggggaa
     4321 aagcaaaaag cagcgctggc tgtcctcttt gccatgaatc ctagggtcta tctcttggat
     4381 gagcccttcg cttctattga ccgcaagagc agaatcgaga tattggagat tctaaaagag
     4441 ttggctcttg atgggaagac agttattttg tgcgaccatg atttatctga ctataaagcc
     4501 tatatcgacc atatggttga gctaagagac ggaaaactaa gggaagtgtt tcaaatccct
     4561 tcctatgaga tgacacaggt tgcttcaaag gaagttgctt ctagcccgga actattccat
     4621 atgaaccgtg tgactggtga gcttggtaat cgccccctct tttcaattgc tgatttcaca
     4681 ttctatcaag ggatttcctg tatcctgggt gacaatggtg tcgggaaatc aaccctcttt
     4741 cgctctattc ttcaatttca aaagtataag gggcgcattg catggaaggg gacagtcctg
     4801 aaaaagaaaa agagtttgta tcgtgatctg actggtgttg ttcaggaagc tgagaagcag
     4861 tttatccgag tcagtctgcg agaggagctt caattagatg gacctgattc tgaaagaaat
     4921 cagcggattt ttcaagcttt acgatatttt gatttggagc aggcagtcga taagagtccc
     4981 tatcaattaa gtggtggtca gcaaaaaatt cttcagctct tgaccatctt gaccagtaag
     5041 gcttccgtga tcttgctaga tgaacctttt gcaggtttgg atgatagagc ctgccattat
     5101 ttttgcaagt ggattgtgga ggagaggaat caaggaagaa gttttctgct cattagtcat
     5161 cgtttagacc ctttgatttc tgtggttgat tattggattg agatgactag tcagggtctt
     5221 cggcatgtga aagaagtgac cattaccaaa ccacttacat ctcagagtag caatacccaa
     5281 ggggaggtga gatagtatgg tcaaagtagc aacccagaca ccgattatca gtctcttctt
     5341 gctgatttta tccttggaaa catctttcat tccttcgatt gctctgaatc tttcggtagt
     5401 cgcattttgt attctcttta tgctctatta ccgtcgattt aaaatgttag cttggatgat
     5461 catacttgcc attttaccat cttttgccaa ctactgggca gttcagttac acggagatgc
     5521 ttcacaggca gtcatgcttg gaacgagggc ctttgtgaca gtttgtatcg gccttgtctt
     5581 tgtttcctct atttcactaa aagagcttct cttgtacttg gctcaaaagg ggctatcacg
     5641 ctcttggtcc tatgccttga ttgtggtatt caattccttt cctctcattc agcaagaaat
     5701 caagtccctc aaagaagctt gcctattacg tggtcaagaa ctgcattttt ggtcgccctt
     5761 gatttacagt aaggttctga tgacagtctt taggtggcgc catctttacc tgagagctct
     5821 gtctgctcat ggatatgacg aacatgcaca gttgaagaat agctatcgga ctttttatat
     5881 tcctaaaaaa acaaaattaa tctacctgct tttcttttta ttgcttcaaa ccagtctatt
     5941 tttataaagg agttattatg gaatttacag atattgcgat ggaattatcc aagaaagctt
     6001 ggcaggcttc ctttcatcat ccctttattt tacaattaca agaggggaat ttagaacctg
     6061 ccattttccg ctattacctg attcaggatg cctactatct gaaggctttc tcagaaatct
     6121 atcatctttt ggctgataag acttcaaacc aagagatgaa aagactcttg aagcaaaatg
     6181 ctcagggttt agtggagggt gagttattta ttcgccaaca atttttcaag gaattggaaa
     6241 tcaacgacca ggaaatggag caacatccaa tcgctccaac ctgttatcat tacatttctc
     6301 atatttatag gcaatttgca gaaccgaact tagccatagc ttttgcaagc ttgcttcctt
     6361 gtccttggtt ataccatgat ataggcaaat cacttaatct taaaccatca ccaaatcctc
     6421 tctatcaaca atggattgaa acttatatta cggatgagtt agagcaacag atcagagagg
     6481 agggagcact ggtcaatcag ctctatcgag aaagtgatga gacagataag caaaaaatgc
     6541 tagatgcctt ccacatcagt gttcatatgg aagccaagtt ttgggagatg gcttaccaac
     6601 accagacatg gaagagcgat ttacagtctt tagaaaaagg agaagaatag aaacatgaga
     6661 aagcgccaac tacaagttca caaattgacc attttatcta tgatgattgc ccttgatgta
     6721 gtccttacac ctatctttcg gattgaggga atggcaccga tgtccagtgt agtcaatatt
     6781 ctagcgggaa tcatgatggg acctgtttat gccttggcca tggctacagt cacagccttt
     6841 atccgtatga cgactcaagg gattccgcct ttagctctca caggagcgac ttttggagcc
     6901 cttctggcag gtctctttta taagtacggt cgaaaatttt atttttctgc cttgggagaa
     6961 attgtgggaa caggtattat tggttccatt gtttcctatc ctgttatggt actctttaca
     7021 ggatcagctg ctaagcttag ctggtttatc tacacgcctc gatttttcgg agcaaccttg
     7081 attggtacag cgatttcctt tattgccttt cgatttttaa tcaagcagga attctttaaa
     7141 aaagtgcagg gatatttctt tagtgaaagg atagactgat gcaggaattt acaaatccct
     7201 ttcctatagg ctctagttcc ctaattcact gtatgaccaa tgagatttct tgtgagatgc
     7261 tggcaaatgg gattttggct ctgggatgca aacctgtcat ggcagatgat ccccgtgagg
     7321 ttcttgattt tactaagcaa agtcaggctc ttttcatcaa tttggggcat ttgtcagctg
     7381 agaaggaaaa agcaatccgc atggcagctt cgtatgcaaa ccaatcttct ctcccgatgg
     7441 tagtagatgc ggttggcgta acgacttcat ccattcgtaa gagcttagtt aaagaccttt
     7501 taaactatag acctacggtc attaaaggaa acatgtcaga aattcgaagt cttgttggct
     7561 taaagcacca cggcgttggg gtcgatgcga gtgctaaaga tcaagaaacg gaggatttgc
     7621 ttcaagtctt gaaagactgg tgtcagacct atcctggtat gtcattctta gttactggcc
     7681 ccaaggacct catcgtttcg aaaaatcagg tcgctgtact ggagaatggc tgtactgaat
     7741 tagactggat aacagggaca ggagatttgg ttggagcctt aacagccgtt tttctcagcc
     7801 aaggaaagac tggttttgaa gcttcttgct tagcagtctc ttatctcaat atcgctgctg
     7861 agaaaatagt tgttcaagga atgggattgg aagaatttcg ttaccaagta ctcaatcagc
     7921 tttcgctcct aagaagagat gaaaattggc tagataccat caaaggagag gcttatgaat
     7981 agagaagcac ttagactata tctggtaacc aatcgctatc aagattccgt ggaaagcttt
     8041 cttgcaaaag ttgagacggc ctgccgttca ggggttacca tagtccaatt gcgagaaaaa
     8101 aatctcacaa ccaatcaata ttatcaactg gcaaaacaag tcaaggaaat aacagatgcc
     8161 tatcaggtac ccttgataat cgatgatcgg ttggatgttt gtcttgcggt tgatgctgct
     8221 ggtctgcata ttggagatga cgaactccca gtttcggttg cccgacaagt cttgggtcct
     8281 gaaaaaatcc tcggtgtcac cgctaaaacg gttaaaagag ctctggaagc ggagaaatca
     8341 ggtgcagatt acttggggac aggagccatt tttccgacaa ccaccaagga aaatgcaccc
     8401 atcaccctga tttcaacctt gaaaacaatt tgccaaacgg ttgccattcc aatagttgct
     8461 attggcggct tgacttcgga gaatattgat cagcttatgg gcactggcat agctggtgta
     8521 gctgttgtac gtgatttgat gcaagcagaa gatattgagg caaaaacgca agccttttta
     8581 acaaagttgc atgacattct ttcctaatta atactcaatg aaaatcaaag agtaaactag
     8641 aaagctagtc gcaggttgct caaagtactg ccttgaggtt ttagataaga ctgacgaagt
     8701 cagctcaaaa cagtgttttg aggttgcaga tggaagctga cgtagtttga agagattttc
     8761 gaagggtata aaaactctct aattccctta aagtgggaac tagagagttt ttttcttaat
     8821 ctttaaaagt tgtatggttg actggaccag aaccatgacc gagttgaggg gcatcttgaa
     8881 tagcttttgt gataaaggcc ttggccttat caactgcctg gtaaagactc ttgcccttgg
     8941 ctagttcagc agtaatcact gcagcaaagg tacatccagt accatgggtg tgacaggttt
     9001 gaattcgtgg gctttcccag acaaattgtt cattcttggt aaagaggaaa tctttagcac
     9061 cacctttgag atgtccgcct ttgataacca cagactgagg accaaattct tttaaaatca
     9121 ggcgaccagc acgctgcatg tcttcggggt catggattga aaaaccaaca atctcttctg
     9181 cttcaggaag atttggcgta ataatagttg ctagaggtag taagtttgtt ttgagatagt
     9241 ctctagcatt tgagtcaatc aaggcatctc cacttgtagc aaccataaca ggatcaagga
     9301 catagggaca atccagtttt ttaagatagg gttggatgat ttccatgatt tcagtagtag
     9361 ccaacattcc agtttttaca gcctgaggtg gaatatcaga aaagacactc tccaattggg
     9421 ctttcaacat ttgaggagaa acgtgctcga ttagctgaac acctctggta ttttgagcga
     9481 caagactggt tacaacagcc attccataga catctttcgc ttggaatgac tttaaatctg
     9541 ccataatgcc agcaccacca ctagggtcag tccctgcaat ggtcaaagca acgggtaaat
     9601 aagtcatcta aaacctccca accaactgaa gtttgtattc ttaagaacaa gctagtctgt
     9661 tttagaaagg caatagaaga ctgctagtcc ccattatcat ttccacttcc atcagctagc
     9721 attacctaga ttgagtcaga gggatctaac agtcgttaat ctcagcttta cgcaccccta
     9781 gtggttgttt tcttttttct ttagtatagc atatttttat tgtttatata gtaatatttg
     9841 actgcaaatc gtttttcaaa tgattaaaat tctatttttc gaagaaaata ttatttctat
     9901 aattgacaaa tgtagatttt aagagtatac tgatgagtgt aattgacaaa tgtagatttt
     9961 ggaggtatgg taatgcagat ttcagatgca gaatggcagg tcatgaagat tatttggatg
    10021 cagggggagc agaccagtac agatttgatt agggttttgg cagagcggtt tgactggtcc
    10081 aagtccacgg ttcagacact tttggctcgt ctggttgaga aagagtgttt gactcggaaa
    10141 aaagaaggca agtcctttgt ttattcagcc cttttaactc tagaccaaag tcgggattta
    10201 cttgtccaag atatcaaaga caaggtttgt tcccgtagga ttaggaattt gttggctgac
    10261 ttgattgtag aatgtgaatt tactcagact gacttggaag acttggaagc tgtgatttca
    10321 gagaagaaat caagtgctgt aacagaagta agatgtaatt gtatgtaaag gagacgtcat
    10381 gttaaatagt attgtaacca ttatttgtat tgcccttatc gcgtttatct tgttttggtt
    10441 tttcaaaaag cctgaaaaat ctggacaaaa agcccagcaa aaaaacggat accaagagat
    10501 tcgagtggaa gtcatgggag gctatactcc tgagttgatt gtcctcaaga aatcagtgcc
    10561 agcccgcatt gtctttgacc gcaaggatcc ttcaccatgt ctggatcaaa ttgtttttcc
    10621 agattttggt gtacatacga acctgccaat gggggaagag tatgtagtgg aaatcacgcc
    10681 tgaacaggct ggagaatttg gctttgcttg tggtatgaac atgatgcacg gcaagatgat
    10741 tgtagagtag gtggagacta tgacagaaat tgtgaaagca agcttagaaa atggcattca
    10801 aaaaatccgt atccgatctg aaaaaggcta tcatccagcc catatccagc ttcaaaaggg
    10861 aattccagct gagattacct ttcatcgtgc tactccttca aactgttata aggaaattct
    10921 gtttgaagaa gaaggtatct tggaaccaat cggcgtagat gaggagaaag tcattcgttt
    10981 tacacctcaa gaattagggc gacatgaatt ttcttgtggc atgaagatgc aaaagggaag
    11041 ctatacagtc gttgagaaga ctcgaaaatc tctatctctc ctgcaacgtt tttggattac
    11101 tagtatcttt actgtgcctc ttgtgattct catgattggg atgttgacag gtagcattag
    11161 tcatcaagtc atgcattggg gaacctttct agcaacaacg cctattatgt tagttgcagg
    11221 taagccatat atccagagtg cttgggccag ttttaaaaag cacaatgcca acatggatac
    11281 cttggttgcg ctgggaactc tagtggctta tttctatagc ctagttgctc tctttgctgg
    11341 tctccctgtt tacttcgaaa gtgctggatt tatcctcttt ttcgttcttt tgggagcagt
    11401 ttttgaggaa aaaatgagaa aaaatacgtc ccaagctgtg gagaaattac tggacttgca
    11461 agctaaaacc gcagaagtct tgagtgatga tagttatgtc caagttcctt tggaacaagt
    11521 caaggtaggc gacctgattc gagtgcgtcc cggtgaaaag attgctgttg atggtgtcgt
    11581 agtagaaggt gtctctagta ttgacgaatc catggtgaca ggtgagagtc tgcctgtgga
    11641 caagacagtt ggagatactg tcattggctc aaccatcaat catagtggaa cgcttgtctt
    11701 tagagcagaa aaagttggct cagagactgt tttggctcag attgtggatt ttgtgaagaa
    11761 agctcagaca agtcgtgcgc cgattcagga cttgacggat aagatttcag ggatttttgt
    11821 cccagtagtt gtcattttag gaatcatgac cttttgggtt tggttcgtct tgctcaggga
    11881 tagtgtggtt gtgcttggag ctagctttgt gtcctctctt ctctacggag tggcggtttt
    11941 gattatcgcc tgtccttgtg ccttgggact tgcaacaccg acagccctta tggtggggac
    12001 aggacgtagt gccaagatgg gggttctcct caaaaatgga actgtcttac aggaaatcca
    12061 gaaagttcaa acccttgtct ttgataagac cgggactttg acggaaggga aacctgtggt
    12121 aacagatgtc atcggcgacg aagtagaagt gtttgggttg gcagcctcct tggaagatgc
    12181 ttctcaacac ccactggctg aggctatcgt taagcgagcg agtgaagctg gacttgagtt
    12241 tcaaactgtt gaaaattttc agaccttgca cgggaaaggt gtttcagggc gaatcaatgg
    12301 aaaacaagtt ttacttggaa atgctaaaat gctggatggc atggatattt ctaatactta
    12361 tcaagataaa ctagaagaac tagaaaaaga agctaagaca gttgtgttct tagctgttga
    12421 caatgaaatc aaaggcttgc ttgctttgca agatattcct aaggaaaatg ctaagctagc
    12481 catcagtcag ctaaaaaaac gtggtctccg aacagtcatg ctgacaggag acaatgctgg
    12541 tgtggcgcgt gctattgcag atcaaatcgg aattgaagag gtcattgcag gcgtcttgcc
    12601 agaagaaaaa gcccatgaaa tccataaact gcaacagtca gggaaagtag cctttgttgg
    12661 ggacggtatc aatgacgctc ctgcccttag tgtagcagat gtgggaattg ctatgggtgc
    12721 tggaacagat atcgccatcg agtcagcaga tttggtgttg acaaccaata atcttttagg
    12781 agtggttcgt gcctttgata tgagtaagaa aacctttcat cgaattctac tcaatctttt
    12841 ctgggctttt atctacaatg ttgtcggaat tccgattgca gcaggagtct tttcaggtgt
    12901 tggactggct ctcaacccag aattggcagg tctagccatg gcctttagtt ctgtatctgt
    12961 tctgaccagt tcccttctgt taaattttag taaaatagac taaaagtaat gactagattt
    13021 ctttgttata aaacagaaac gacaacactt tcaaaactgt attagaatag agaatagaga
    13081 gttttgagca gatttttaga aaagtcagca taaatatgat acagtggaat agtaaaaatt
    13141 tggagaacgt ttccaattct atgtaatcgt attctccaag tttaaaaaaa ttgaaggaga
    13201 gttatcatta tgactcaagg gaaaattact gcatctgcag caatgcttaa cgtattgaaa
    13261 acatggggcg tagatacaat ctacggtatc ccatcaggaa cactcagctc attgatggat
    13321 gctttggctg aagacaaaga tatccgtttc ttacaagttc gccacgaaga gacaggtgct
    13381 cttgcagcgg ttatgcaagc taaattcggc ggctcaatcg gggttgcagt tggttcaggt
    13441 ggtccaggtg cgactcactt gattaacggt gtttacgatg cagctatgga taacactcca
    13501 ttcctagcga tccttggatc acgtccagtt aacgaattga acatggatgc tttccaagag
    13561 cttaatcaaa acccaatgta caacggtatc gctgtttaca acaaacgtgt agcttacgct
    13621 gagcaattgc caaaagtaat tgacgaagcc tgccgtgctg cagtttctaa aaaaggtcca
    13681 gctgttgttg aaattccagt aaacttcggt ttccaagaaa tcgatgaaaa ctcatactac
    13741 ggttcaggtt catacgaacg ctcattcatc gctcctgctt tgaacgaagt tgaaatcgac
    13801 aaagctgttg aaatcttgaa caatgctgaa cgcccagtta tctatgctgg atttggtggt
    13861 gttaaagctg gtgaagtgat tactgaattg tcacgtaaaa tcaaagcacc aatcatcaca
    13921 actggtaaaa actttgaagc tttcgaatgg aactatgaag gtttgacagg ttctgcttac
    13981 cgtgttggtt ggaaaccagc caacgaagtg gtctttgaag cagacacagt tcttttcctt
    14041 ggttcaaact tcccattcgc tgaagtttac gaagcattca agaacactga aaaatttatc
    14101 caagtcgata tcgaccctta caaacttggt aaacgtcatg ctcttgacgc ttcaatcctt
    14161 ggtgatgctg gtcaagcagc taaagctatc cttgacaaag taaacccagt tgaatcaact
    14221 ccatggtggc gtgcaaacgt taagaacaac caaaactggc gtgattacat gaacaaactc
    14281 gaaggtaaaa ctgagggtga attgcaattg tatcaagttt acaatgcaat caacaaacat
    14341 gctgatcaag acgctatcta ctcaatcgac gtaggtaaca ctactcaaac atctactcgt
    14401 caccttcaca tgacacctaa gaacatgtgg cgtacatctc cactctttgc gacaatgggt
    14461 attgcccttc ctggtggtat cgctgctaag aaagacaatc cagatcgcca agtatggaac
    14521 atcatgggtg acggagcatt caacatgtgc tacccagacg ttatcacaaa cgttcaatac
    14581 gaccttccag ttatcaacct tgtcttctca aatgctgagt acggcttcat caagaacaaa
    14641 tacgaagata caaacaaaca cttgtttggt gtagacttca caaacgctga ctacgctaaa
    14701 attgcggaag ctcaaggagc tgttggattc acagttgacc gtatcgaaga catcgatgca
    14761 gttgttgcag aagctgttaa attgaacaaa gaaggtaaaa ctgttgtcat cgatgctcgc
    14821 atcactcaac accgtccact tccagtagaa gtacttgaat tggatccaaa acttcactca
    14881 gaagaagcta tcaaagcctt caaggaaaaa tacgaagcag aagaactcgt accattccgt
    14941 ctcttcttgg aagaagaagg attgcaatca cgcgcaatta aataattcct ctcgccgaaa
    15001 atcaaatatg aaacttgtag actttattct ttgattttca tagagcagaa ct
//
LOCUS       DAVKQB010000049        14321 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKQB010000049 DAVKQB010000000
VERSION     DAVKQB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14321
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.049"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(247..555)
                     /locus_tag="VW528_001674"
     CDS             complement(247..555)
                     /locus_tag="VW528_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9912472.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEVHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(552..860)
                     /locus_tag="VW528_001675"
     CDS             complement(552..860)
                     /locus_tag="VW528_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9912473.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(865..2160)
                     /locus_tag="VW528_001676"
     CDS             complement(865..2160)
                     /locus_tag="VW528_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9912474.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(2178..3587)
                     /locus_tag="VW528_001677"
     CDS             complement(2178..3587)
                     /locus_tag="VW528_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9912475.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWLERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            3745..4473
                     /locus_tag="VW528_001678"
     CDS             3745..4473
                     /locus_tag="VW528_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9912476.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            4932..5126
                     /locus_tag="VW528_001679"
     CDS             4932..5126
                     /locus_tag="VW528_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9912477.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(5492..6802)
                     /locus_tag="VW528_001680"
     CDS             complement(5492..6802)
                     /locus_tag="VW528_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9912478.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALKLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            7039..7911
                     /locus_tag="VW528_001681"
     CDS             7039..7911
                     /locus_tag="VW528_001681"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9912479.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            8042..8873
                     /locus_tag="VW528_001682"
                     /pseudo
     CDS             8042..8873
                     /locus_tag="VW528_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            8984..9910
                     /locus_tag="VW528_001683"
     CDS             8984..9910
                     /locus_tag="VW528_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9912480.1"
                     /translation="MDLSQTMPSYAQVVSLYDDTSISVSNNETDKVLAGSLYTDTNEQ
                     GLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQFQTKIVRVINDE
                     DDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQKNKKYTVLS
                     QQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIGVMKAVGYQ
                     NRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFELNLLSVLG
                     TLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            10087..11094
                     /gene="gap"
                     /locus_tag="VW528_001684"
     CDS             10087..11094
                     /gene="gap"
                     /locus_tag="VW528_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9912481.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(11161..11997)
                     /locus_tag="VW528_001685"
     CDS             complement(11161..11997)
                     /locus_tag="VW528_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9912482.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWKEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            12123..14318
                     /locus_tag="VW528_001686"
     CDS             12123..14318
                     /locus_tag="VW528_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9912483.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
BASE COUNT         4432 a         2547 c         2884 g         4458 t
ORIGIN      
        1 aagctagtat agtaaactga aataagatat aaacaaataa attggagctt aacatccatt
       61 tccagcaatt ttttagaaac tacagtggac tattctagat tcaacatatt ataaaaacta
      121 gagtaaaaga aaaggattgg atcttgtgta atgcaggatc caatcctttc aatcattttg
      181 tccaactttt ggaggttcct acaatgtagc cgtcattaat aaagacagat gggaacgaca
      241 gtgttcctat ttattttgat agagatcgat gaattcttta gatagcaact gaataatctc
      301 cgttgaagcc atttggtctt ctgcatgcat aaatagcaag gagaatccta ttttttctcc
      361 agtagcttct ttttgtatga gattagagtg aatcttgtgc acttctacta aggagtcttc
      421 cgcttcttca actttaattt tcgcttcttt taaatttcct gccttagcta gttggatggc
      481 ttcaataaag gatgatttgg ctgctccact attggcaatg agctgaaaac agatatattc
      541 catttcttct gtcatcttat ttctcctatc catgcaagtg cttgttccag aacttttgct
      601 ccattcatca ttccgtaatc ccgcatatca atggtatcta cagggatatt tcctgcaatt
      661 tctttcacag caagtaactc ataacgaatt tgtggcccaa ttagaatgac atctgcttca
      721 tggatattct ttttcgcctc tgtcattgat tttgcttgga tagaaatttc aatcccacgt
      781 tcagtcgcac tttgttgcat ttttttaaca agcatacttg tcgacattcc cgcattacat
      841 actaataaaa tttgtttcat aatcttaacc ttccatttct tgttcaacaa ctttgtcatt
      901 aactttgata aatggaatgt atagaagaac tccaagtgca aagatgatga attgaactag
      961 aactgctctc acgtcccctg ctgttgctaa ccatgcattt aagaatactg gtgtagtcca
     1021 aggaacttgt ataaatgcag gactcatgaa tcctgtaact gttgctaagt agctgattaa
     1081 aataccaagg actggaactg tgataaatgg aatagctaat gaaatgttat aaacgattgg
     1141 gtaaccgaat aatactggtt cattgatatt gaagatacca ggtccaaaag ataatttagc
     1201 cacgttttta gagacagcat tgcgactcac taagaatgtt gctattaata aacataatgt
     1261 agatccacta ccacccatta aagcgaatgt ttgtatttgt gataggttga tgatgtgtgg
     1321 aatggcttgt ccattatttg ctgcagtgat gttttcagta atgttaatta atagtaatgg
     1381 ttctaggatg gcactgtaaa taactgcttg gtgaatacca aatagccata acatatttcc
     1441 taaagagtaa ataataatga ccccgattaa gcttgtacca atatgacgaa ttggttcttg
     1501 aataaagatt gtaatgattg agattaagtt cattccagtt atattgaata ataatgctga
     1561 aacaacccca aataaggaga tgacggtcat gactggaagt aatacgctaa atgatctact
     1621 aacagctggt ggaatatttt caccaaggtt catttgtaaa gctttaacgt ttgataattc
     1681 aatgaataat tctgttgcaa taatcgctac gataaccccg gcgaacattg cgcctgtacc
     1741 tgtgttgttg aatgaaagaa cacctgaaat gtttaccgca tcttttgctc cgtcaggaac
     1801 tacagaaact gtatttggca tcatcacaat taaagaaact aatgatagca ttgatgctgc
     1861 taacgggttt tcgaaatctc tgtttttagc taagaaataa ccaaccatta cagcaataat
     1921 catacctgaa atacttaaag taccgtttgc aattgttatt ccccaatatt ggaatcttgt
     1981 taatgtatcc ccttggaaaa tccacttaaa taccgtgttg ttcaaaagaa cgattaaacc
     2041 tgccaaaata tataatggca ttactgttac gaatgcatct cttagggttt ttaaatgaat
     2101 ttggttccct agtttaccag caaaggtggc aaattggtct ataaattttg agtttttcat
     2161 atgaatatct cctctcttta ctcctcaatt tctacttcaa aaccgttatt gtcacttact
     2221 ttgcgatacc atcttcctga gctcttgatg gttctctttt gcgtttctaa atcaacggag
     2281 ataaatccgt aacgattctt atatgcatta ttccaagacc agcaatcaaa tgcggtccaa
     2341 gcgtggtatc caaaacagtg acttccctct tcaatggctt tatgtagcca tcttaaatgt
     2401 tcttcataaa attcaatacg atacacgtca tcgataactc cattttcatc gataaaccgt
     2461 gcttcgtttt caacacccat tccgttttca ctgataaacc atgggatatt accatattct
     2521 tccttcacaa tcatagcaat atcataaatg gctttcggaa aaatttccca accacgatat
     2581 ggattcatgc gacgctccag ccattcatac tctttgaagt attggtctgg catccagggc
     2641 gtctgatatt cttcaggatt tgcttgtgct tgaacacgtt ttggatggta gtagtttact
     2701 ccaagaaaat caaccgtatt tgatttcatc agttgaagct ctttttcggt atgactccat
     2761 aacacgccat ctctctctag ctgttttacc aatctttctg ggaaagttcc tttaacagct
     2821 ggattcaaga agactttgtt aaagaagtca tctgtaaatc gacttgcttc taagtcttct
     2881 ggagaattac ttcttggata agcaggtgtc aagtttaaaa taatcccaat ctttccatca
     2941 agttctaatg agcgatatag ttgaatcact tttgcactag caaggtttag attatagatg
     3001 acttgtacgg cctcttttcc ctttcctttt agatttggat aatggaaagc atataagtat
     3061 cctgcttctg gaatgaccat tggctcattg aaagttgtcc agtaatgaac cttatctcca
     3121 aagcatgtga aagcagtctt ggcaaacttc acgaataact ccactacatg tttgctttcc
     3181 caaccaccgt atttttgaag aagttccact ggtaaatcaa aatgatgtaa attcatcaca
     3241 agatccatct ggttcttttt agcttcttca atgatggcat tgtagaaagc aatacctttt
     3301 ggatcaggct cacctgtctc taaattcttg atgagtcgac tccattggat ggaagtgcgg
     3361 aaagaattaa ctccaatttc cttcatcaag tggaaatctt cggtgtaagt atgaaaaaag
     3421 ttactggcta caagaggtcc gacattatcg aaaaaatctt ctggatgcgt tttgaaccag
     3481 tagtccatca cattttcatg tacttttcca tattgacctt ctgtctgagg tccgctagaa
     3541 gcagctcccc accaaaatag attaggaaat actaactttt ttttcatgct caacctcctt
     3601 tctattattc tatacataaa gtatagcata attttaaaat ttgtctagac atttttaaaa
     3661 attgttgata catatcttgt gtttgcctca tttcttgaac tttgttatac tactattaga
     3721 atattataga gagaaccaga ttaaatgagt acaaaatatt tatttattta caatgagatt
     3781 cgtgaaaaga ttctttgtaa taaatatacc atgaacgaac aattgcctga tgaaatgaca
     3841 ttagctaaac agtttgcctg tagtcgaatg acgatcaaaa aagctttaga cttgttagtt
     3901 tctgagggct taatttttag aaaacgtggg cagggaacct ttgttctctc tcgtggcagc
     3961 tcaaaaagaa aattaatcgt tccagaaaga gatatccggg gactgacaaa aatatctgaa
     4021 gatgctcatt ctacaattga ctcgaggatt attcacttca aattagaatt tgcaaatgaa
     4081 tttttagcag aaaaactaca ggtcgctttg cagagtccag tttataatat ttaccgtctg
     4141 cgtattattg acggtaaacc ttatgttctg gaacaaactt atatgagtac tgatgttatt
     4201 ccaggtatta ctgaagatat tttacaaaaa tcgatttaca attacattga aggaaagtta
     4261 ggattgcata ttgccagtgc tacaaaaatc ttgcgagctt cttctagttc agaaaatgag
     4321 caacattact tgcagctcct tccaacggaa ccggtatttg aagtagaaca agtggcttat
     4381 ttggataacg gaactccgtt tgagtactcg attagtcgtc atcgctatga tttatttgaa
     4441 tttaattctt ttgcattacg acattcctcc taggagaaaa tgtgaaaatg aagccaatct
     4501 tttacagact ctagtttaag aaaaatttaa aacagggcaa gaaggtccca tctatgctta
     4561 aatggtttct cttttctaaa taagatggct ttaaaagagt gatcgtttta tccatcatgt
     4621 tgaaaaatat cttcgtatag cttatagagt aggtactgaa attgttcacc tgatctactt
     4681 cttatagtta tttagtttta aatagtgttt caaatattct tacactgacg agaagttttt
     4741 gagtcttttc ttgtaacaca tatagtatac tgatgttaga atagtagact gtgacttcta
     4801 acaaattgct agaaatgaat ttcaatctcc caatttattt gttcatatct tcttttaata
     4861 tattaaataa attctgaatc ataatcattt aaaaaaattt tattttttat ttttcattac
     4921 gaataatata gatgaagggg aaagagtatg aaaacagaac ggtttctttt gctattagtt
     4981 caaaaggaga aaaaatgaaa gtagaaaata tttcgtatag ggtggatcat cgtatattgt
     5041 ttgataatat ttcttttgat acttcgagtt caggcgtgac attaattact ggtaaaaatg
     5101 gtacaggaaa gtcaacttta ctatagtaga ttgaaactag aatagtacac atctacttct
     5161 aaaatattgt tagaaatcga tttgactatc ctgatctatt tgtcctgttc ttatttcatt
     5221 tcactacatc tcaaattgag tatgacgaag tgcgctccca tgtcctggga acgcactttc
     5281 ttcatatttt tcatattctt gaatccatcg ataaagacta ttgggatgaa tttttaaagt
     5341 tgaactaatt atttttacag gatgaggttt acagcagaga gtttgaaggc tttatcaaag
     5401 gtttttcttg gcataatgac ttttcctcgt ttccacttaa ttttgtgtct actttattat
     5461 accaagtcca cccttaagtt agataatgaa tctaacttaa ggaagctaga aggatgagaa
     5521 tccaggtggt caagagtccc aaacttaagc tgatagggac acccagaata atttgctttt
     5581 tgaaggcaag accgcgttcc tctatattgg gaagtgagag ttgaatgaga gaaccagctg
     5641 atgaaaaggg tgagatatta gtagatagag cgccaataac ggtggctgtt gtgagtaagt
     5701 gaatatcaat ctgaggattt tgagcactga tgatagcaat gatgggaaag agggctggag
     5761 ctacaacgga tagggtggaa ctaaagagtg acatcactcc ggctatcaca caaaagaaca
     5821 gaggtaacca gaaatgagga atggttgttg tcatgaggtg ccctatcagt gtgactaaac
     5881 ctgacttgac cgctagagac attagtaagc tcatgccgca gagcatgata attgtagccc
     5941 agggaacctt agctaaaatg gcttcttgct tccctaattt gagctttaag gcgaggcaga
     6001 ccatgagtat tgagacaaag ccaatatcaa atgttttttg ataagtagct atccaggcga
     6061 tgtttgggaa aatgagatgc aacaagggaa aaagccaaac caaaaccatg ctgctgatca
     6121 tgagcaaggt ggtttgtctt tgaaccttgc tgaggagtgg tggttggtca atagtcaagg
     6181 atgagtttgt tcttccctta ctatagtgac tgtaacagga taataaaagc aagacgatga
     6241 gtgggtagat aatgctgacg ataaagatat gattgccaag tgaaaaagct tgctcttccc
     6301 atcccatttg cttaaacagg ccttgaaaga caatgcctga gctactggtt atcaaattag
     6361 cccctcctga agctccccaa ttgacggctt gagctccaat caaagggtgt ttgtccgctt
     6421 tttgacagag ggtaatcgct agaggacagc aaacggccat agtagtgaaa aatccagcac
     6481 ctaaagcaga caaaagggtt gccatcaggt ataaaatcat gtagagggcg ttagggtggg
     6541 tgcgtgtgcg gtagagaatg tgttgagcca aaacatcaag agtaccgtta gttgttgcaa
     6601 cgttataaaa gagagagacg ctaaaaatgg taaaaaagag tgaggttggc caaaaatgaa
     6661 gaagttcttt ggggcttaat cccatgagag tggttgcgat gaggtaagaa aaagcaatag
     6721 ccagcaggcc aatattgatt ttggtgcggt aaccaattcc aatggctaga gcaatggcgc
     6781 taatcattat taaatgaatc attgaattgt cctttcagtt agaatatgga aataggttgg
     6841 caattttggc gctcaaaaga ccacctgttt cctattggta cttgactatt atacacaaaa
     6901 aaaatacaat tgtcttgaca attgtattga cacttgttag aattaaaaat gaggtattca
     6961 ggcttgtgat ttcctatgga agttaatagt aattgcctct agtgcttaca agtgatatta
     7021 aaaatagagg acttagtgat gtcaatcatt tcaactgatt taaccccttt tcaaatagat
     7081 gatacattga aagcagcctt gcgagaagat gttcattccg aagattacag taccaatgcc
     7141 atttttgatc atcatggcca agctaaggtg tcgctttttg ccaaggaagc tggtgtttta
     7201 gcggggctaa ccgtttttca aagggttttt accctatttg atgccgaggt gaccttccag
     7261 aatcctcatc aatttaagga tggggatcgt ttgactagtg gcgatttggt tttagaaatc
     7321 ataggctcgg tgagaagtct cttaacatgt gaacgcgttg ccttgaattt tttacaacat
     7381 ttatcaggga ttgcttcgat gacagctgct tatgtagaag ccttaggcga tgattgcatt
     7441 aaggtatttg atactcgaaa aactactcct aatttacgtc tttttgagaa atatgccgtg
     7501 agagttggcg gtggctataa tcatcgcttt aatttatcag atgctatcat gctaaaagac
     7561 aatcacattg cggcagtagg tagtgttcaa aaggcaattg ctcaagcgcg tgcctatgcc
     7621 ccttttgtga aaatggtcga ggtggaagtg gaaagccttg ctgctgccga agaagctgcg
     7681 gcggcgggtg ctgatattat catgttggat aatatgtcat tggaacagat tgaacaggcc
     7741 attaccctaa ttgcaggacg ttctcggatt gaatgttctg gaaatattga tatgaccact
     7801 attagccgtt ttcgtggttt agcgattgat tacgtctcca gtggtagttt aacccatagt
     7861 gctaagagtc ttgatttttc catgaagggg ttaacctacc ttgatgtcta agttgtaaaa
     7921 taaactaact ttttaaagga tgtctttcct ctagaacgag ttttatgtca gatagtttaa
     7981 acgcctcttc aaatatagta aaatgaacca aaaatagtac acaatgtggt ataatctttt
     8041 tatggcatat tcaatagatt ttcgtaaaaa agttctctct tattgtgagc gaacaggtag
     8101 tataacagaa gcatcacacg ttttccaaat ctcacgtaat accatttatg gctggttaaa
     8161 gctaaaagag aaaacaggag agctaaacca ccaagtaaaa ggaacaaaac caagaaaggt
     8221 tgatagagat agacttaaaa actatcttac tgacaatcca gacgcttatt tgactgaaat
     8281 agcttctgaa tttggctgtc atccaactac catccactat gcactcaaag ctatgggcta
     8341 cactcgaaaa aaagaaccac acctactatg aacaagaccc agaaaaagta gccttatttc
     8401 ttaagaattt taatagttta aagcacctat cacctgttta gattgatgaa acaggatttg
     8461 atacttattt ttatcgagaa tatggtcgct ctttaaaagg tcagttaaga agaagatatc
     8521 agaggatttc tttggttgca ggtctaacaa atgaatggtg agttaatcgc tccaatgact
     8581 tacgaagaga cgatgacgag cgactttttt gaagcatggt ttcagaagtt tctcttacca
     8641 acattaacca caccatcggt tattattatg gataatgcaa gattccatag aatgggtaag
     8701 ttagaactct tgtgtgaaga gtttgggcat aaacttttac ctcttcctcc ctactcacct
     8761 gagtacaatc tattgagaaa acatgggctc atatcaaaaa gcacctcaaa aaggtattac
     8821 caagttgcaa taccttttac gaggcttttt tatcctgctc ttgtttcaat tgactatagt
     8881 tcacggatac agttgggaaa gaagctatta aatatttgga gagtcctttt gggatgaaca
     8941 tgtcaaatat tactatagat gggcaagaaa agatgggcaa gaaatggatc tgagtcaaac
     9001 gatgccttct tatgctcaag tagtgagtct ttatgatgat acaagtatct ctgttagtaa
     9061 taatgagacg gacaaagtgt tggctggctc cctctatact gatacaaatg aacagggatt
     9121 aacgattcca agcagtttac taaaaaattg gaatgaacag acaggaaaaa atttgacagc
     9181 taatgatctt attggcaaat cagtctcagc cagcattgta gaaagtgctg ccgaaactag
     9241 taagattgct caatttcaaa cgaagattgt acgtgtaatc aatgatgaag atgacatgga
     9301 ggacagcaac agtttcatgc tgtctcatca aatggaaacg attttgaaag aggctggatt
     9361 tacgaaagct gtatcttatt ttatcttgga actcaaagat ccatcacaga caaaagtagt
     9421 aacagaagaa ttacagaaaa ataagaagta tactgtgctt tctcaacaga gagttcttga
     9481 tattgtgatt acctttattc gtgttattca gggattattg attgtgcttt catcacaagc
     9541 tattgtggta gcagcggtta tgattggtat cattatttac atcaatatca tgcaacgttc
     9601 caaggaaata ggtgtcatga aagcagttgg ttatcagaat cgtggtgtca aaggaatttt
     9661 tatttacgag gctatctgga ttgtaggcat cgccttgctg ctggcatttt tggttgcaca
     9721 aggggtggga agtttggcga atgcgattgt aagtcacttt tacccatcca tcactaaggt
     9781 ttttgaatta aatcttttat ctgttttagg aactctagtt ttcgctctat tacttggtta
     9841 tgtctcagcc tacttcccgg cgcgtaagat tagtaaaatg gatcctgtag aatcgttacg
     9901 ctatgaataa taagggattc cttgatttac agtcttaaaa agaaactcac atagaaaaac
     9961 aatggccata cttgcaattc aactcaaata gttgtataat aggtttgttg aaaggtgatt
    10021 tgtagtgatt caagttactc ttttcacaat aaaaatttca tgattttcat aaggaggaaa
    10081 tcactaatgg tagttaaagt tggtattaac ggtttcggac gtatcggtcg tcttgctttc
    10141 cgtcgtatcc aaaacgtaga aggtgttgaa gttacacgca tcaacgacct tacagatcca
    10201 gttatgcttg cacacttgtt gaaatacgac acaactcaag gtcgtttcga cggtactgtt
    10261 gaagttaaag aaggtggatt cgaagttaac ggtaaattcg ttaaagtttc tgctgaacgt
    10321 gatccagagc aaatcgactg ggctactgac ggtgtagaaa tcgttcttga agctactggt
    10381 ttctttgcta agaaagaagc agctgaaaaa caccttaaag gtggagctaa aaaagttgtt
    10441 atcactgctc ctggtggaaa cgacgttaaa acagttgtat tcaacactaa ccacgacgtt
    10501 cttgacggta ctgaaacagt tatctcaggt gcttcatgta ctacaaactg cttggctcca
    10561 atggctaaag ctcttcaaga taactttggt gttgttgaag gattgatgac tactatccac
    10621 gcttacactg gtgaccaaat gatccttgac ggaccacacc gtggtggtga ccttcgccgt
    10681 gctcgcgctg gtgctgcaaa catcgttcct aactcaactg gtgctgctaa agctatcggt
    10741 cttgtaatcc cagaattgaa cggtaaactt gacggatctg cacaacgcgt tccaactcca
    10801 actggatcag ttactgaatt ggtagcagtt cttgaaaaga acgttactgt tgatgaagtg
    10861 aacgcagcta tgaaagcagc ttcaaacgaa tcatacggtt acacagaaga tccaatcgta
    10921 tcttcagata tcgtaggtat gtcttacggt tcattgtttg acgcaactca aactaaagtt
    10981 cttgacgttg acggtaaaca attggttaaa gttgtatcat ggtacgacaa cgaaatgtca
    11041 tacactgcac aacttgttcg tactcttgaa tacttcgcaa aaattgctaa ataattcttg
    11101 agttgataga aagcaaggct ttgtggtctt gctttttata tggaaaaatg gatgacacga
    11161 tcatccattc ttttttaatt ctttttcaaa tgtatttgaa agggtagtga aagttagctt
    11221 ctctaaagta agtgggtggg taaaggaaag tcggaaggca tgaagcataa gccggcttgt
    11281 ctttgattta ctattataga gagggtctcc caggatagga agattatgat gcgaaaggtg
    11341 cacacgaatc tgatgggttc gccctgtctt tagcttgcaa tgagccaagg aagtcttgtt
    11401 tgagaattgc tttaatctgc ttacatgcgt ttcagcatat tgcccatttt ttgcatcaac
    11461 tattcttttt ctacgatcat ggcgatcacg tccaattttg tctctgaaaa caagttcttt
    11521 tctgttgata tttccatcaa ctagagccca atattctcta gaaatctctt ttttctccaa
    11581 taagcgattg agaatgggca ggataaaagg atttttggca aagagaacta agccactggt
    11641 ttccatgtcc agacgatgaa cgacatagca ggtttggcca acataggtac tgacatggtt
    11701 aagaagggca atttcgtttg gttgattacc atgcgttttc atcccctctg gtttgtttac
    11761 aataatcaag tgttgatctt gataaacttc ctgcactaag tctgggttgc cccaagggat
    11821 cgtcttttgg gaataatctt cctcgtcaaa agtcaactgg caaacatctc caggatttac
    11881 gatttccttc cagtggactt cttcttgatt tatcaaaata tgtttcttga ttctcaaaaa
    11941 atgacggatt tttctaggga tgaggagttg ttcctcaagt aattgcttta ctgtcatttg
    12001 aggtagagag gcgggtaatg taaatgtgaa ttgcatacag atattgtaac aaaaaaagcc
    12061 ctatttggat aggaaatagc taaattcttg tcttcctatg atgaagatga taaaataaac
    12121 gcatgaaatt agataaatta tttgagaaat ttctttctct ttttaaaaaa gaaacaagtg
    12181 aactagagga ctctgattct actatcttac gtcgctctcg tagtgatcga aaaaaattag
    12241 cccaagtagg tccgattcga aaattctggc gtcgttatca tctaacaaag attatcctta
    12301 tactaggttt gagtgcaggc ttgctagttg gaatctattt gtttgctgta gccaagtcga
    12361 ccaatgtcaa tgatttgcaa aatgccttga aaactcggac tcttattttt gaccgtgaag
    12421 aaaaagaggc tggtgccttg tctggtcaaa agggaaccta tgttgagctg actgacatca
    12481 gtaaaaactt gcagaatgct gttattgcga cagaagaccg ttctttctat aaaaatgacg
    12541 ggattaacta tggccgtttt ttcttggcta ttgtcactgc tggacgttca ggtggtggct
    12601 ctaccattac ccaacagctg gctaaaaacg cctatttatc gcaggatcaa actgttgaga
    12661 gaaaagcgaa agaatttttc cttgccttag aattaagcaa aaaatatagt aaggagcaaa
    12721 ttctaaccat gtaccttaac aacgcttatt ttggaaatgg tgtgtggggt gtagaagatg
    12781 cgagtaagaa atactttgga gtttctgcat cagaagtgag tctggatcaa gctgcgactc
    12841 tggcagggat gctcaagggg ccggaactgt ataatccctt gaattccgta gaagattcta
    12901 ctaatcggcg cgatactgtc ttgcagaata tggttgcagc aggatatatt gataaaaacc
    12961 aagaaaccga agctgctgaa gttgatatga cttcgcaatt gcacgataag tatgaaggaa
    13021 aaatctcaga ttaccgttac ccctcttatt ttgatgcggt ggttaatgaa gctgtttcca
    13081 agtataatct aacagaggaa gagattgtca ataatggcta ccgcatttac acagagctgg
    13141 accaaaacta ccaagcaaat atgcagattg tttatgaaaa cacatcgcta tttccgaggg
    13201 cagaggatgg aacgtttgct caatcaggaa gtgtagctct cgaaccgaaa acagggggag
    13261 ttcgtggagt tgtcggtcaa gttgctgaca atgataaaac tggattccgg aatttcaact
    13321 atgcaaccca atcaaagcgt agtcctggtt ctacaattaa gcctttagtt gtttatacgc
    13381 cagcagttga agcaggctgg gctttgaata agcagttgga taaccatacc atgcagtatg
    13441 acagctataa ggttgataac tatgcaggga tcaaaacgag tcgagaagtt cctatgtatc
    13501 aagccttggc agaatcgctt aatctacctg ctgttgccac tgttaatgat ttgggtgtcg
    13561 ataaggcttt tgaggcaggc gaaaaattcg gactcaacat ggaaaaggtc gaccgtgttc
    13621 ttggtgtcgc cttgggaagc ggtgttgaaa ccaaccctct tcaaatggct caagcatacg
    13681 ctgcctttgc aaatgaaggt ttaatgcctg aagctcattt tattagtaga attgaaaatg
    13741 ctagtggaca agttattgcg agtcataaaa attcacaaaa acgggtgatt gataagtctg
    13801 tagctgacaa gatgaccagt atgatgttgg ggactttcac caacggtacc ggtattagtt
    13861 catcgcctgc agactatatt atggcaggga aaactggaac aactgaagca gttttcaatc
    13921 cggagtacac aagtgaccag tgggtaattg gttatactcc ggatgtagtg attagccact
    13981 ggcttggctt tccgaccact gatgaaaatc actatctagc tggctctact tcaaacggtg
    14041 cagctcatgt ctttagaaac attgccaata ccattttacc ttatacgcca ggaagtacct
    14101 ttacggttga aaatgcttat aagcaaaatg gaattgcacc agccaataca aaaagacaag
    14161 tacaaaccaa tgataatagc cagacagatg ataatttgtc tgatattcga gggcgtgcgc
    14221 aaagtctagt agatgaggct agccgggcta tctcagatgc gaagattaag gaaaaggctc
    14281 aaacaatatg ggattcgata gtcaatctat ttcgctaaga t
//
LOCUS       DAVKQB010000050        14138 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKQB010000050 DAVKQB010000000
VERSION     DAVKQB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14138
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.050"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            81..878
                     /locus_tag="VW528_001687"
     CDS             81..878
                     /locus_tag="VW528_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9912484.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSIFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGSLIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1026..1148
                     /locus_tag="VW528_001688"
     CDS             1026..1148
                     /locus_tag="VW528_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU9912485.1"
                     /translation="MTRDFKFETLQLHAGQVVTPATESRAVPIYQTTSFVFDDT"
     gene            1170..2285
                     /locus_tag="VW528_001689"
     CDS             1170..2285
                     /locus_tag="VW528_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEU9912486.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDIDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGSSCHNLSYIRDVGAATFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETLSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGDEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPNQIRLSIGLENVEDLIEDLRLALEKI"
     gene            2381..3655
                     /locus_tag="VW528_001690"
     CDS             2381..3655
                     /locus_tag="VW528_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9912487.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3665..4543
                     /gene="truB"
                     /locus_tag="VW528_001691"
     CDS             3665..4543
                     /gene="truB"
                     /locus_tag="VW528_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9912488.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4784..5086
                     /locus_tag="VW528_001692"
     CDS             4784..5086
                     /locus_tag="VW528_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9912489.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5194..5832
                     /gene="udk"
                     /locus_tag="VW528_001693"
     CDS             5194..5832
                     /gene="udk"
                     /locus_tag="VW528_001693"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9912490.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5960..7300
                     /locus_tag="VW528_001694"
     CDS             5960..7300
                     /locus_tag="VW528_001694"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9912491.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7278..7490
                     /locus_tag="VW528_001695"
     CDS             7278..7490
                     /locus_tag="VW528_001695"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9912492.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7487..8362
                     /locus_tag="VW528_001696"
     CDS             7487..8362
                     /locus_tag="VW528_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9912493.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8355..9170
                     /locus_tag="VW528_001697"
     CDS             8355..9170
                     /locus_tag="VW528_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9912494.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDVKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9163..9594
                     /locus_tag="VW528_001698"
     CDS             9163..9594
                     /locus_tag="VW528_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9912495.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9601..11268
                     /gene="recN"
                     /locus_tag="VW528_001699"
     CDS             9601..11268
                     /gene="recN"
                     /locus_tag="VW528_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9912496.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGVPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLHLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11270..11998
                     /locus_tag="VW528_001700"
     CDS             11270..11998
                     /locus_tag="VW528_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9912497.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12077..13900
                     /gene="lepA"
                     /locus_tag="VW528_001701"
     CDS             12077..13900
                     /gene="lepA"
                     /locus_tag="VW528_001701"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9912498.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQVLGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14059..14138)
                     /locus_tag="VW528_001702"
                     /pseudo
     CDS             complement(14059..14138)
                     /locus_tag="VW528_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
BASE COUNT         4173 a         2577 c         3366 g         4022 t
ORIGIN      
        1 ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa
       61 aaaagattta ggagtttatt atgatttctt cagaatttat ctcaaagatt gaatttgctt
      121 gcaataagaa agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatat
      181 tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta
      241 ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg
      301 cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg
      361 gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct
      421 tgttcaacct tatcggatcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg
      481 cgaatctgac acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa
      541 atgaattggt cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt
      601 catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca
      661 tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga
      721 aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact
      781 ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg
      841 ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg
      901 tgcttttttc attttcaaaa taaggtaata gctatttctt atatcaaaat atagaaaact
      961 gatatttgta aactataact caaggtgcta caatatcctt aataaaataa tatggaggtc
     1021 accttatgac tcgtgatttt aaatttgaaa ctctacaact acatgctggt caagttgtga
     1081 ctccagctac tgagtctcgt gcagtgccga tttatcaaac aacatctttt gtttttgatg
     1141 acacgtagga aggtgctgat ctgtttgcct tgaggaaacc agggaacatt tatactcgta
     1201 tcaccaatcc tacaacagct gcccttgaag gtggtgttga agcgctagca acagcatcag
     1261 gtatgactgc agtgacttat acgattttgg cgcttgccca tgctggtgac catgtagtgg
     1321 ctgcttcgac tatttacggt ggaaccttca atcttttgaa agaacccctt cctcgttatg
     1381 gtatcacaac aacctttgtc gatattgata atttggagga agtagaagca gctatcaaag
     1441 acaataccaa gcttgtcttg attgaaacct tgggtaactc cttgattaat attccagacc
     1501 tggaaaaact ggcagagatt gctcataaac atcaaatccc acttgtgtca gacaatactt
     1561 ttgcaacacc ttatttgatt aacgtcttct ctcatggcgt tgacattgcc attcactctg
     1621 cgactaagtt tatcggtggg catggtacaa ctattggagg aataattgtc gatagtggtc
     1681 gttttgactg gacggcttca gggaaattcc ctcaatttgt tgacgagggt tcaagctgcc
     1741 acaatttgag ctatattcgt gatgtgggtg cagcaacctt tattatagct gttcgagttc
     1801 aattgcttcg tgatacaggt gcagccttgt caccattcaa tgctttcctc ttgctacaaa
     1861 gacttgaaac tctttcactt cgtgtggaac gccatgtaca aaatgctgag acaattgttg
     1921 attttcttgt caaccatcct aaggtagaaa aggtaaatta tccaaaactt gcagatagtc
     1981 cttatcatgc cttggctgag aaatacttgc caaaaggtgt cggttcaatc tttaccttcc
     2041 acgtcaaagg tgacgaggaa gaagcacgca aggtcattga taatttagaa atcttttctg
     2101 accttgcaaa cgcggcagat gctaaatcgc ttgttgtcca tccagcaaca accactcacg
     2161 gtcaattgtc agaaaaagac ctagaagcag caggtgtcac accaaaccaa attcgtctgt
     2221 caatcggtct tgaaaatgta gaagatttga ttgaagactt acgcttggcc ttggaaaaaa
     2281 tttaaagtaa aagaagataa acagtgggtt ttgactcact gtttttgatt ttccctcagg
     2341 catgatataa tggttacaga agtctagaaa gaggaatgta atgaacgaaa tcaaatgtcc
     2401 caactgtggg gaagtcttta cagtaaatga aagccagtat gctgaacttt tgtcccaagt
     2461 gagaacggca gagtttgata aggaactaca cgataggatg aggcaggaac tggccttggc
     2521 tgagcaaaag gctatgaatg agcaacagac taaactggct cagaaggatc aagaaattgc
     2581 gcaattacag agtcagatcc aaaactttga tacagaaaaa gaattggcca agaaagaggt
     2641 tgaacagaca agccatgagg ctctcttggc taaggacaag gaagtacagc tcttagaaaa
     2701 tcagttggct accttgcgtt tggagcatga aaatcaacta caaaagaccc tttctgacct
     2761 agaaaaagaa cgggatcagg ttaaaaacca actacttttg caggaaaagg aaaatgaatt
     2821 atctttggct tctgttaagc aaaactacga agcccagctc aaggcagcta gtgaacaagt
     2881 cgagttttat aagaatttta aggctcaaca atctacaaaa gcgattgggg aaagcctaga
     2941 acagtatgca gagagtgagt ttaacaaggt tcgtagtttc gcctttccaa atgcttactt
     3001 tgagaaggat aacaaggtct cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg
     3061 tgatgaaaat ggagttgaaa tcatttctat catgtttgag atgaaaaacg aagcggacgg
     3121 aacagagaag aagcacaaga atgcagattt ttacaaggaa ttggacaagg accgtcggga
     3181 gaagaactgt gagtatgccg ttttggtgac catgcttgag gctgataatg actactttaa
     3241 cacagggatt gttgacgtca gtcacgagta tgaaaaaatg tatgttgttc gtcctcaatt
     3301 ctttatccaa ttgattggtc tcttacgtaa tgcggcgcta aattccctaa aatacaagca
     3361 ggagttggcc ttggttcgcg agcaaaatat tgacattacg cattttgagg aagatttgga
     3421 tgcctttaag ctagcttttg ctaagaacta taattcagct tcgactaact ttggaaaagc
     3481 tattgatgaa atcgacaagg ccatcaaacg catggaagag gttaagaaat tcctgaccac
     3541 atctgaaaac caactccgtt tagctaacaa caaattggaa gatgtctctg ttaaaaaatt
     3601 gacccggaaa aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg agtagaaagc
     3661 aaaaatgaac ggtattatta acttaaaaaa ggaagcagga atgacctcgc atgatgcggt
     3721 ttttaaactg cgtaagattt tgggaaccaa gaaaattggt catggtggaa ccttggatcc
     3781 ggatgtggtg ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg tcgagtttat
     3841 gcaggacgag ggtaagatct atgaggggga aatcactctg ggctattcca cgaagactga
     3901 ggatgctagt ggggaagtgg tcgcagaaac ccctgttttg tctctcttgg atgaaaagct
     3961 tgttgatgaa gcgattgcta gcttgactgg gcctattact cagattcccc ctatgtattc
     4021 ggcagttaag gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc aggaagtgga
     4081 gcgtccagaa cgtcaggtga ccatttatca atttgagcga acaagtccga tttcttatga
     4141 tggccaactt gcccgattca cttttcgtgt aaaatgcagt aaagggacgt acatccgtac
     4201 tttgtcagtt gatttgggtg aaaagcttgg ttatgcggct catatgtccc atttgactcg
     4261 tactagtgct gctggcttac aattagaaga cgctcttgcc ttggaggaaa ttgctgaaaa
     4321 agtagaggct gggcaattag attttctcca tcctttagag attgggacag gtgaccttgt
     4381 caaagttttc ctaactccag aagaggctac agaagttcgc tttggtcgtt ttattgagct
     4441 agaccaaacg gacaaagaac tggctgcctt tgaagatgat aaattgttag ccattctaga
     4501 aaaacggggc aatctctata agccaaggaa ggtttttagc tagatcgttt aggaataaaa
     4561 atcgggtgat agataacaat tgcttgataa aaccccatac taatagtaga atggttttgg
     4621 gaattataat attccaattg ttgcgagttg taggtactca aataatctat atagaaattt
     4681 agaggtgtga aatgaagcaa tttaaaattc tttcagataa atatttagag tccattacag
     4741 gttctgatgg gaacttaggc ccaggatttg gtgtgataat tccatgatgc gaaatgagtt
     4801 tcgagaaagg gtggagcaac ttcttcaaca aaaagaaata aatgaaaata gtgagttgag
     4861 tcacctgttt cgtcttgcta tacaaaattt agacagaaat gaaaaatacc aatcggtcat
     4921 ggccaatttg agtcaagggt tgtcacttta cctcatgacg catcattacc aggcacctaa
     4981 gtctgtcatt gattttggtt tatggattgc caaagctcct agccaggaaa gagggagact
     5041 ggctttcttg cagatgcttg ctcaaaccct acagggcttt cgttaaaact gtcgaacatc
     5101 agagggaaga ctctggtgtt ttttgctgat gagactataa attttgtaaa aaatgtgata
     5161 gaatagacga cggataaaat aacggaggat agcatgcaaa atagaccaat cattattgga
     5221 gtgacaggtg gttctggtgg tggtaaaacc agtgtttcaa gagccatttt atcgcatttt
     5281 cctgatgaaa agatttccat gattgagcat gattcatact acaaggatca gtctcatttg
     5341 acctttgaag agcgtgtcaa aaccaactat gaccatcctt ttgcctttga tacagacttg
     5401 atgatcgagc agattaagga attgttggca gggcgtccgg tggacatccc gacctacgac
     5461 tatacagagc atacacggag tagcaagacc tatcgtcagg aacctcaaga tgtctttatc
     5521 gttgagggta ttttggtctt ggaggacaag cgtctgcgcg atttgatgga tatcaagatt
     5581 tttgtggata cggatgacga tgtgcgcatt attcgtcgta tcaagcgtga tatggaggag
     5641 cgtggccgta gccttgatag cgttattaac cagtacttag gtgtggtcaa accaatgtac
     5701 caccagttta tcgagtcaac taagcgttat gctgatatcg tcattcctga aggggttagc
     5761 aataccgtgg ctatcgacct gttgacgacc aagattgcaa agattttgga agaagctcga
     5821 aacagcaaat aatcacatgt ggaggccttg cctccttttt ctatttttcc ctcataatgg
     5881 tacataaatg gagattttta ggcatatttt tggtataata atacccatga aagagcaagt
     5941 aaatgaatag taggtggaga tggaaaagta tttatcggta acaactttga ccaagtatct
     6001 gaaaatgaaa ttcgataaag acccatactt ggaacgggtc tatttaactg gtcaagtttc
     6061 caactttcgt aaacgaccta ctcaccaata tttctcccta aaggatgacc atgcagttat
     6121 tcaagcgacc atctggtctg ggatttatca gaaattaggg tttgacctgg aagaaggaat
     6181 gaagatcaat gtgattgggc gtgtacaggt ctatgaacca agtggtagct actccatcat
     6241 cattgaaaaa gctgagcctg atggggttgg ggcgcttgcg attcagtttg aacaacttaa
     6301 gaaaaaattg acagaagaag gcctgtttca agaacgcttc aagcaagctc tgccccaatt
     6361 ttctaagaga attggtgtag taaccagccg tagtggagcc gttattcgag atattatcac
     6421 gaccgtcagc aggcgattcc caggtgttga catccttctt tatccgacca aggttcaagg
     6481 tgaaggggct gcggaggaaa ttgctagaaa tattgcgcgt gctaatcaac gggacgattt
     6541 ggatttgctc attattggtc gtggaggtgg ttctattgag gatctctggg cctttaacga
     6601 agaaattgtg gtacgagcta tttttgaatc tcgtttgcca gttatctcta gtgtggggca
     6661 tgagacggat gtgaccttgg cagattttgt ggcagatcga cgcgctgcaa cgccaacagc
     6721 ggcggctgaa ctggcaacac ctgtgaccaa gttggatgta ttagctcatt tgcaaaatca
     6781 ggaaaaacgg atggtaacgg cagtccgaaa tgttctatct aagaaacaag aggctttgaa
     6841 aaaatgcagt cagtctgtta tctttagaca acctgagcgc ttgtatgacg gttatttgca
     6901 acgcttggac caactgcaac tgcgtttgaa acaaagtttg cgaactcgga tttctgataa
     6961 caaacaatta gttcaagcaa gaactcatca attagtacaa ttatcacctg ttaccaaaat
     7021 ccaacgctat caagaccgtt taggacagtt ggacaagctc ttaggtagcc aaatggcgtt
     7081 agtttatgac gccaaggttg ctgaggccaa gcgactttcg gaagctttgc tcatgttgga
     7141 tactagccga atcgtggcgc gtggttatgc tattgtcaaa aaagaagagt ccgttgtaga
     7201 ttcggttgag agtttgaaga aaaaagacca agtaacgctt ttgatgcgag atggtcaagt
     7261 agaattagag gttaaagatg tcaaaacaaa agaaatttga ggaaaatcta gcagaactgg
     7321 aaaccattgt ccaaagtttg gaaaatggtg aaattgctct ggaagatgcg attactgcct
     7381 ttcaaaaggg catggtcttg tcaaaagagc tccaagctac gctggacaag gctgaaaaga
     7441 ccttggtcaa ggtcatgcaa gaagacggaa cagaaagtga ttttgaatga aaaagcaaga
     7501 aaaattagct cttgtcgagt cggctttgga agatttttat ggagaccagc agtttgcctc
     7561 tagtttacgg gagtctgttc tctattctat tcatgctggt ggcaagcgta ttcggccttt
     7621 tctcttgtta gaagttctgg aagccttgca ggttaccatc aaacctgctc acgcgcaggt
     7681 agctactgcc ttagagatga ttcatacagg gagcttgatt cacgatgacc ttcctgctat
     7741 ggatgatgac gattatcgaa gagggcggtt aaccaatcac aagaaattcg gtgaagctat
     7801 ggccattttg gctggagatg ctttattctt agacccatat gccttgattg cgcaggcaga
     7861 tttgccaagt cagatcaagg tggacttgat tgccaactta tcccttgctt caggtagtct
     7921 gggtatggtg gcagggcaag ttttggatat ggagggcgaa caccagcact tgtctctgga
     7981 agaacttcag actattcatg ccaataagac tgggaagtta ctagcctatc ccttccaagc
     8041 ggcagctatt atagctgaat tgtcacctga aatgcaggtg aagctgaaaa ctgtgggtga
     8101 attgattgga cttgcttttc aagtcagaga tgatgtactg gatgtgacag ctagttttga
     8161 ggaaatcggc aagacacctc aaaaggatct gcaggcagaa aaatcaacct atcctgcctt
     8221 gttgggcttg gaagagtcca ttgccttttg taaccaaacc ctggatcaag ctaatgaaaa
     8281 attagaagaa attgcccagc agcttccctt tgaaacagaa tcgattgtaa gtgtagtaga
     8341 aagtttgaga atcaatggct aaggaaagag tggatgtact agcttataaa caggggttgt
     8401 ttgaaacgag agagcaggcc aagcgaggtg tgatggctgg cctagtcgta gcagtcctta
     8461 atggagaacg gtttgacaag ccaggagaga aaattccaga tgacaccgaa ttaaaactca
     8521 agggggagaa actcaagtat gtcagccgtg gtggtttgaa actggaaaag gccttgcagg
     8581 tctttgattt gtcggtggat ggcgcgacta cgattgatat cggggcctct actggaggtt
     8641 ttaccgatgt catgctacag aatagtgcca agttggtctt tgcagtcgat gttgggacca
     8701 atcagttggc ttggaaatta cgccaagacc cacgagttgt cagcatggag cagttcaatt
     8761 tccgctatgc tgaaaagact gatttcgagc aggagccgag ctttgccagt attgatgtga
     8821 gtttcatttc ccttagtctg attttgccag ccttgcaccg tgtcttggct gatcaaggtc
     8881 aggtggtagc acttgtcaaa cctcagtttg aggcaggacg tgagcagatt gggaaaaatg
     8941 gaattattcg agatgttaag attcatcaga atgtccttga atctgtaaca gctatggcag
     9001 tagaggcagg tttttcagtc cttggcttgg acttttctcc catccaaggt ggacatggaa
     9061 atattgaatt tttagcgtat ttgaaaaaag aaaagtcagc aagcaatcag attcttgctg
     9121 agattaaaga agcagtagag agggcgcata gtcaatttaa aaatgaataa aaaagaaaga
     9181 cttgaaaaaa ttagaagatt ggtgacagat tatcaaatcg gcacgcaaga agaaattgta
     9241 gaacatttga aagaagcagg tatcactgcc actcaggcga cggtatcccg agatatcaaa
     9301 gagttaggta ttgtcaaaat tcctttgaga gacaacacct atgtctacga attaccaaaa
     9361 tcaatcgtaa aaagtctgca actggctgaa gacaatatcg aatcggctga attgatggat
     9421 aagatgatca atctccaagt tattccagga aatacggctt ttgtaaaagc tcagttaatc
     9481 gagacttttg cagacaagat ttttagctgt ttgactgatg atagctcgat tttagtcatt
     9541 gccagaagtg gaagtctagc agaggaaatc tttgaacaag taaaaaattg gtaggtctat
     9601 atgttacttg aaatttcgat aaaaaacttt gccattattg aggctatttc cctcaatttt
     9661 gaaaagggga tgactgtcct gactggtgaa acgggtgcag ggaagtcgat tatcattgat
     9721 gccatgaata tgatgttggg agctcgtgcg acgacagatg ttattcgtca tggtgtacca
     9781 aaggcagaga ttgaggggct tttctcagtt gagaatagtc gtcttttaca ggaaattttt
     9841 gatgagcaag gtttagaatt aggtgatgaa attatcatcc gtcgggaaat cttgcaaaat
     9901 ggtcgtagta tcagccgtgt aaatggtcag atggttaatc tgtctgtttt gcgagctatt
     9961 gggcaacatc ttgtagatat tcatggtcag catgaccatg aggagttaat gcgtccccaa
    10021 ctgcatatcc agatgttgga tgaatttggt gatgccgctt tttgggactt gaaagaaacc
    10081 tatcaaacga gttttgatgc ctatcggaaa atgcgcaagc aggttctgga agtcaagaaa
    10141 aaccaacagg aacacaaggc acgtatcgaa atgttggaat ttcaaatggc agagattgag
    10201 gcagcaaact tgcaggctgg agaagacttg gctctcaatc aagagcgaga taaactcctc
    10261 aaccacaaaa atattgcgga tacactgacc aatgcctaca gtatgttgga caatgaagat
    10321 ttttcaagtc tggccaatgt tcgttcagcc atgaatgaca tggaaagtgt cgaagagtat
    10381 gaccctgaat accgtgaaat ttcaagctct ctgtctgaga cctactatgt tttagaagac
    10441 attagcaaac gtttggaagc tatcattgag gaccttgatt ttgatggcaa tcgcctcatg
    10501 caggttgaga atcgtttgca cctccttcat actattactc gtaagtatgg tgggactgtt
    10561 gatgatgttt tgctttattt tgccaagatt acggaagaat acaatctctt gacaggcaat
    10621 aatctttcgt ctgaggacat ggaagcagag cttaagaagt tggaagtcaa tcttgtcaat
    10681 ttggcaggtc aacttgcttc tgctcgtcat aatttggctc agcaactcga agctgagatt
    10741 aaacaagaac tgcaagatct ttatatggaa aaagcccagt ttcaggttca ttttagtaag
    10801 ggaaaattca gtcgtggggg aaatgaaatg gttgagtttt atatttcaac caaccctgga
    10861 gaagacttta aacccttggt taaggttgct tctggagggg aattatctcg tctcatgtta
    10921 gccattaagt ctgccttttc acgtaaagaa ggcaagacta gcattgtctt tgatgaggtg
    10981 gatacgggag tttcaggtcg tgttgctcaa gctattgctc agaaaattca taaaattggt
    11041 cagcatggtc aggttttggc tatctcccat ttgccacaag taattgcgat tgcagattat
    11101 caattcttta ttgagaagat tagtaatgac cattcaacgg tttcgactgt tcgtctcttg
    11161 acagtcgaag agcgagtgga ggaagttgcc aagatgttgg caggtgatga tgtgacagaa
    11221 gcagccctga cgcaagccag agaattgttg agaaacaggg agaaataaga tgacagatta
    11281 ttatgtaatt ggagatgttc acggaaaagc tgggatgctg gaagaccttc ttaaaacctg
    11341 ggatggtcag acccagttgc tctttctagg tgatttgatt gacagaggtg aggatagtca
    11401 ccgtgttctt gaaatggtca aggacttggt ggacaatcaa ggggctatct gtttgtctgg
    11461 aaaccacgag tatatgtttt tgacttggct agatgaccca gaagaaagtt atgaccatta
    11521 tcgtcgcaat ggtggagata caaccattaa ctctatccta ggtcgtccct tggatgcacc
    11581 agttgatgga gtagaagatg ccaagcgggt tgccgctgaa gcgtcagact tggtcgaatt
    11641 cattcgtcaa atgccatttg tagtagagac agacaagtat atatttgttc acgcaggtat
    11701 tgatttgact ttggacgact ggcatgaaac aacagattat aaaaaagtat ggcttagaaa
    11761 accattccac ggagctgaaa atcatactgg aaaaaccatt gtcttcggtc atacaccggt
    11821 ctatggtttg ttacagcaag aacgcggtac atctgagctt tggactacag aagatggcaa
    11881 gattggaatg gatggaggag ctgtttatgg tggtgtcctt cacgggattg tctttacaga
    11941 ccaaggaatg acagaacacc actttatcga aaatgacggc tttgtcgctg aagattagta
    12001 ctcctagcag ggtatggtct tgtcaaaata tcaaaaacaa tttataataa atagataccc
    12061 tgaaaggaag agaatcatga acttagaaga attgaaaaaa cgacagggga agatccgtaa
    12121 cttctctatt atcgcccata ttgaccacgg gaaatcaact ctagcagacc gcattttgga
    12181 aaaaacagag acggtttcaa gtcgtgaaat gcaggcccag cttttggata gcatggatct
    12241 agagcgggaa cgtggaatta ctattaagtt gaatgccatc gagttgaatt acactgcaaa
    12301 agatggggaa acttatattt tccacttgat tgacacacca gggcacgttg actttaccta
    12361 tgaagtttca cgttcgctag ctgcctgtga gggtgctatt ttggtggtcg atgcggctca
    12421 aggaattgag gctcaaactc ttgccaacgt ttatctggct ttggataatg atttggaaat
    12481 catgcctatc attaataaaa ttgacctgcc ggctgcagat ccggagagcg tgcgtacaga
    12541 gattgaagat gtgattggtt tggatgccag tgaagcagtt ttggcttctg ccaaggctgg
    12601 tattgggatt gaagaaatcc tcgagcaaat tgtagaaaaa gtaccagcac caacgggtga
    12661 tgtgacggcg ccacttaagg ccttgatttt cgactctgtt tacgatgctt accgtggggt
    12721 tatcctccaa gtgcgtgtca tggacggagt ggtcaaacct ggtgataaga ttcagctcat
    12781 gagcaatagt aagacctttg atgtggccga agtcggtatt tttacaccaa aagcggttgg
    12841 tcgtgatttc cttgcgactg gtgatgttgg ttacattgcg gcgtctatca agacggttca
    12901 ggatactcgt gtgggtgata ccgttacctt ggcaaccaat cctgcggcag aaccattaca
    12961 tggttataag cagatgaatc ctatggtctt tgcgggtctc tacccaatcg aatcaaacaa
    13021 gtacaatgac ctgcgtgaag cgcttgaaaa attgcaactg aatgatgcta gtcttcagtt
    13081 tgaaccagaa acatctcagg tacttggatt tggtttccgt tgtggatttc ttggacttct
    13141 ccatatggat gttatccagg aacgtttaga gcgtgagttc aatattgatc tcatcatgac
    13201 agctccgtct gttatttaca aagttaattt gaccgacggt gagtctatgg atgtgtctaa
    13261 cccatctgag tttccagacc caactaagat tgcgaccatt gaagagcctt atgtcaaggc
    13321 gcaaatcatg gtaccacagg agttcgtcgg agcagtaatg gagctagctc agcgtaagcg
    13381 tggggacttt gtgactatgg actatattga tgataaccgt gtcaatgtta tctatcaaat
    13441 tcctcttgct gaaatcgtct ttgacttctt tgataaactt aagtcttcga cacgtggtta
    13501 tgcaagtttt gactacgaat tgtcagaata tcgcccatct aagctagtga aaatggatat
    13561 ccttctcaac ggagacaagg tggatgccct cagctttatc gttcataagg actttgctta
    13621 tgaacgtggg aaactcatcg ttgataagct caagaaaatc atccctcgcc aacaatttga
    13681 ggttccaatc caagcggcta ttggacacaa gattgtcgct cgtactgata tcaaggccct
    13741 tcgtaagaac gtacttgcta aatgttatgg tggtgacgtt tctcgtaagc gtaaactcct
    13801 tgaaaaacaa aaagctggta agaaacgcat gaaatccatc ggatcagttg aagttccgca
    13861 agaagccttc ctcagcgtct tgagtatgga tgaagaataa gttatcagaa atgcctttcc
    13921 ttgttcacag gaaatttata taagaatcaa tagtaaagca ggcttagatg gtctgctttt
    13981 ttagttttta tactcttcga aaatctcttc aaaccacgtc agcgtcgcct taccgtatgt
    14041 atggttactg acttcgtcag tttcatctac aacctcaaaa ctgtgttttg agtaacctgc
    14101 ggctagcttc ctagtttgct ctttgatttt cattgagt
//
LOCUS       DAVKQB010000051        13900 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKQB010000051 DAVKQB010000000
VERSION     DAVKQB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.051"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..513
                     /locus_tag="VW528_001703"
     CDS             1..513
                     /locus_tag="VW528_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9912499.1"
                     /translation="MIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSDHEWM
                     LTDIKNFLTKHKAWVIDGNYSWCCYQERMQEADQIIFLNFSPLTCLFRTFKRYLKYRG
                     KVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRELDQF
                     LDKKRKSYNS"
     gene            complement(536..1246)
                     /gene="deoD"
                     /locus_tag="VW528_001704"
     CDS             complement(536..1246)
                     /gene="deoD"
                     /locus_tag="VW528_001704"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9912500.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1904..2416)
                     /locus_tag="VW528_001705"
     CDS             complement(1904..2416)
                     /locus_tag="VW528_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9912501.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            complement(2441..3421)
                     /locus_tag="VW528_001706"
                     /pseudo
     CDS             complement(2441..3421)
                     /locus_tag="VW528_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            complement(3897..4706)
                     /locus_tag="VW528_001707"
     CDS             complement(3897..4706)
                     /locus_tag="VW528_001707"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9912502.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            complement(4706..5263)
                     /locus_tag="VW528_001708"
                     /pseudo
     CDS             complement(4706..5263)
                     /locus_tag="VW528_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            complement(5265..6476)
                     /locus_tag="VW528_001709"
     CDS             complement(5265..6476)
                     /locus_tag="VW528_001709"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9912503.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            complement(6490..7173)
                     /gene="rpiA"
                     /locus_tag="VW528_001710"
     CDS             complement(6490..7173)
                     /gene="rpiA"
                     /locus_tag="VW528_001710"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9912504.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            complement(7455..8327)
                     /locus_tag="VW528_001711"
     CDS             complement(7455..8327)
                     /locus_tag="VW528_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9912505.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            complement(8469..9326)
                     /locus_tag="VW528_001712"
     CDS             complement(8469..9326)
                     /locus_tag="VW528_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9912506.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(9480..10214)
                     /locus_tag="VW528_001713"
     CDS             complement(9480..10214)
                     /locus_tag="VW528_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9912507.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESRKVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            complement(10214..10897)
                     /locus_tag="VW528_001714"
     CDS             complement(10214..10897)
                     /locus_tag="VW528_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9912508.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            11087..11317
                     /locus_tag="VW528_001715"
     CDS             11087..11317
                     /locus_tag="VW528_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9912509.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            11546..13804
                     /locus_tag="VW528_001716"
     CDS             11546..13804
                     /locus_tag="VW528_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9912510.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKTEVKSSEKEK"
BASE COUNT         4123 a         3137 c         2541 g         4099 t
ORIGIN      
        1 attatcggat attctggttc tggtaagtca actctagcag aaaagttatc taactactac
       61 tccattccaa aactgcacat ggacacactc caatttcaac ctggttggca agacagtgac
      121 cacgaatgga tgttaaccga tataaaaaac tttctcacca agcataaagc ttgggtcatc
      181 gatggtaatt attcttggtg ctgctaccaa gaacgaatgc aagaagctga ccaaatcatc
      241 tttctcaatt tttcgccatt gacctgtctc tttagaacct ttaagcgtta tcttaaatac
      301 cgtggaaaag tcagagaaag tatggcggca gattgccctg aacgctttga ctgggagttt
      361 atcagatgga ttctttggga tgggcgtagc aaaactcaaa aagaaaatta ccaaaaactt
      421 tgccaagaat attcacataa agtcactatt cttcgaaatc agagagagct agatcaattt
      481 ctggataaga aaaggaagtc ctacaattca taaagggctt cctttttggc tataattatt
      541 ctgcaatcaa ggtttccaaa ccaaccttca tcatatcagt gaaggtattt tgacgttctt
      601 ctgcagttgt gtcttcgtct ggattgacca agctatcaga gatggtcatg atagctagcg
      661 catcaacatg gtattgggca gcaagatagt aaagagctgc tgcttccatt tccacagcct
      721 tgactcccca tttaccaagc tcgatattct tttcaaagta atttgagtaa aagacatcag
      781 atgacaaaac gttcccaacg tgagtagtca taccaagttt tttggcgata tggtaggctt
      841 tatcaagcaa atcaaagcta gcaatttgtg gaaaatcgta ctgtggccag tcattacgaa
      901 cgatgtttga gttggttgca gccgcctgcg ccaaaactaa ttcacgaaca tgaacctctt
      961 cattcaaaga acctgcagtt cccacacgaa tcaatttctt cacaccgtag tctacgatta
     1021 actcacgcgc ataaatcgaa atagatggca ttcccatccc agttcccatg acagatacac
     1081 agtgaccctt gtaagtacca gtgtaaccaa acatgttacg cacttcgtta aaacaaacag
     1141 catcatcaag gaaattctcc gcaataaact tagcacgaag aggatcccca ggaagaagaa
     1201 ttttatcagc aatttcaccc tgctgagcag caatatggat agacataatt tatgatacaa
     1261 agagcgagaa gaaaacgact gaaaattagg aacctgacga gaaatcctga tttttcagtc
     1321 agattatcta ttttccgagt tttccgctcg tgttcaaatc aaaacacacg ctctaccttt
     1381 ctttatttta tattttatat tgagaaagat accaaaccca tcaaaaagcg aagggaaaat
     1441 aggagttggg cgcagtgagc gatgctcgct agaccaacta tctttttccc actgctttta
     1501 gggtggggtc aattcctttc tttcttaatt ttgatttaga ggagagtcgc ccgtattcag
     1561 ttcagcgaat acagtttacc catcctttcg tttttatttt tagaaaagtt ttctactcgt
     1621 gttcaaatta gaacacgcgc tctacctttc tgtttatact cttcgaaaat ctcttcaaac
     1681 cacgtcaacg tcgccttgga ttatatatgt gactgacttc gtcagtctta tctacaacct
     1741 caaagcagtg ctttgagcaa cctgcggcta gtttcttagt ttgctctttg attttcattg
     1801 agtattaatt ttgttctttc acagagagct acctgagttc tattcaagct caggtagtac
     1861 tttcttataa actagacaaa ctaactgtca ttgtaccatc agattacaag acatcatcgt
     1921 cactcacctt ggaattcaat gtcgtacccc aatgggtaat tttacggtgg ggttgagcta
     1981 aaattggtct gttttcatag attgtttgcc atctattcca tagtaggccc gtctttttct
     2041 caatcttaac tcgcagattt ctcatatttt ctttgattgg gaggttgagg acaaaacctg
     2101 cagtctggtt gcgaccgttt ccttcccaag aatgactacg aacaacttgg tttccatctt
     2161 tatctactgt aacttcttcc caagttatgg agtagcgggc aatgtaagct ccactgtgtt
     2221 gaattgtcaa tgttttatct ttcacaggag tctgactgat tggttgaact ggcttagaaa
     2281 cttgtgtcgc cgtttcagca ttcgtagcta taaagcggaa aacttcaact tctgcaagac
     2341 tgagggcatt atagccttct agcccaatcc gaacatagcg cgctttttta tggttaatcg
     2401 taatttgtgc tgacacatct ttaggagata ttatacgttt tcactcaatt tctttaccag
     2461 aactgtctaa aagaatgaca tcaaagtttg ccaatctatc ctgggcagtg tctgttcggt
     2521 tgtaaatatt gatttggcga atggtttctt ctttagccaa atctacttgc caccaaggct
     2581 tagattggaa gtttgtatga gtgacagaat tgtgaccata gttaccatcg actttgccat
     2641 ccacagctct tctagcatct cctccgaaag ctgtcgaact ttgagtaacc tgttttccta
     2701 aggcaatatt ttccattggt tcagcgcttg gttgactaga agattgaggt agagcctgtt
     2761 tcttaaagaa tcgcacttta tccaatttcg gatcacgata accatgttta tccaaggtag
     2821 cttgtttacc aatattaaag ttaggaatat cattcattgg actttcaaaa tagccacgag
     2881 aacggtgttt aaagcgtgaa ttacctgtaa atgtaacaat atcacgatta ttatgaatca
     2941 agggagtcac aacattgtca ttatcaacca cataatgctt aatttttcca ctaacagcta
     3001 atttacgact ttccaaacca acatctcaga aaccattctt agcatcccaa acagttctgg
     3061 aagtaatcac tttaggagca ctgaaagttg tcactttcct caatacatgg ttataaaaat
     3121 caggatattt ttggtaagct tcaaccgctg caatctgagc taggtaacat ccaagagaat
     3181 gacctgtcat atacaactta gtaatactta tatccttggc taattcccca acaaccttgc
     3241 ggatatcatc cgcttgagct ggctctatta tctctacttt tgactcttcc cttttgtttt
     3301 caagaacttc acttgcttcc ggaagcgttt gaggagctag tcttggctcc tctctaagag
     3361 gagtttctag ttttggagca tcattttctg actctttcaa ttcctcctta gaatctagca
     3421 cacctattac cacttcagat gttacatctt gctttacttc attggctgaa attccagctg
     3481 gagctagaaa tcaaagccga ctacaacaga tacaactccg atacttaatt ttttatgcca
     3541 aagcgcataa ttcgttgacc aattttcatt atttcttatc tttcttttcg tttatttaca
     3601 agcagactca agcgaaatct cattgtgcta tatatgataa gactgagaaa ttcagaaagt
     3661 caattcattc tcatctctcc ttatcatact agtctacttg gctaggaagc taatgctagt
     3721 atcctagaat cttgtctttc acaatgctgg ctatcagatc agacacacct attcttccgc
     3781 ttctttcagt tataagagca ggaagaagct catagtattc ctctgtttct tctttaatcc
     3841 ggataggcag tcaatcctga aaaaacagag gcactccccc ttttaaatct tttttcttac
     3901 aattcagcaa gaatcgcttt aagcaagcct ttgaaatcac ctttaacacg ttcagtcact
     3961 tctacaactt cttcgtgatt gagttcttct tggaaaccgg ccgcaaagtt agtgatacat
     4021 gaaattccca gaactttcaa gccagagtgg gccgccacga taacttcagg aaccgtagac
     4081 ataccaactg catctgctcc cagtgtctta taggaacgaa tttctgctgg tgtttcataa
     4141 gtcggaccag taactccgat atagacacct tcatcaagct tgatattaag ttttttagcc
     4201 acttcatggg cagtggcacg gtattctggt gtgtaggccc tagacatatc tgggaaacgt
     4261 gggccaaagt catccaagtt ttcacccatc aatggatttt gccccgtcat gttgatatgg
     4321 tctgagatag ccatcaaggt accaggacca aatccgatac cgccagctgc attggttaca
     4381 ataacacctt cacatccaag aactttcatc acacgaactg ggaaagtcac cacttccaga
     4441 ggattccctt catagaaatg gaaacgccct tgaagagcca agaccttgcg acctgccagt
     4501 tcaccatata ccaatttacc agcatgaccg actactgttg aacggcccca gtttggaatc
     4561 tcagcatagt ctactacaac tggattttcg atttcttctg ccaattctcc aagtcctgac
     4621 ccaaggatta gaccgaactc aggggctgca attccctttt ctttcaggaa agtagcagtt
     4681 tcatggattt tgtttaaaaa tgtcattatg tgtctccttt attatttttt agcatttgac
     4741 caattttgtc aaaggtttta gcattacgaa tagtaatgtg gcggtagaaa ggcaccttca
     4801 gcaagtactt atgataggca gtcttagaat aggattcttc agaaaatttc ccccagaaaa
     4861 tcccaagttt tctaaaataa agcacttcat ctttcagctc taaactttca actgtcgcga
     4921 tgacttggtc cacatccaaa ccctcagtgt aaaagagaaa atcttttcgt gccaagtctc
     4981 tgctccacca agctggtaga ttttcaagtt ccgcctcaaa gtcctctaga ctcagtaaag
     5041 aaaagctctg aataaatgga taatggactg caaagaaagt ctctagcttt taaaccaatt
     5101 gggctttgga atctatcgaa gtaaagaaaa tattgccact gttgatgtag ctctcaacct
     5161 tttccagtcc caagtttgtc aattcttgac gaagctccgc cataacgacc ttattcttac
     5221 taccaacatt gatacctctc accagcaaag catagcgcgt catcttatac caatttatct
     5281 aagaaacttt ccccaatcat agcagtttcc acaccaaagt tatcggcaac agtcgctgaa
     5341 atatctgcaa aatgtcctac tggaatgaga ccatttcctt taaaggcagg gctatatgcc
     5401 aacaatggaa tatattcccg agtgtgatcc gtccctgcat acgttgggtc atttccatgg
     5461 tccgcagtaa tcaagagaag gtcattctct ctcatagctg cgataatttc aggtaagcgt
     5521 tcatcaaact catgcaaaca atcacggtaa ccgtgagcat tacgacgatg gccgtaaagg
     5581 gcatcaaagt caactaggtt tgtgaatgag aatccttttt caaactcagc aagtcccata
     5641 gtcttcaata gtgtatcaat tccatgacta tttgacttgt tgtgacccat gtcatggttg
     5701 ataccagcac cgttaaagat atcgttgatt ttacccacag cataagtatc gataccagcc
     5761 tcattcaatt tatccaaaac agttggggaa aatggagata cagccaagtc acgacggttt
     5821 gccgtacgag tgaagttacc tggttcacct acataagggc gagcaatgat gcgaccaaga
     5881 agggcaggac gctcaagggt aatcgaacga gcgtattcac agatacggta caattcatcc
     5941 aaaggaataa tgtcttcgtg ggcagcaatc tgcaaaacag ggtcagctga agtatagata
     6001 atcaactctc cagtttccat ctgacgtggt ccaaaatcat cgataacagc cgttcctgaa
     6061 taaggtttgt tggcttcacg aataaccttg cgtcctgaga attcttcgat ttttgtcagg
     6121 atttcttctg ggaatccgtt ccagaaagta tcgaaaggct cagtaatgtt gagtcccatg
     6181 atttcccagt gtccagtcat agtatcctta ccaagagata cttcctctaa ttttgttgca
     6241 tatccagttg gattgatttc agctgctaca gtcttaagag gagtttcacg aggaatattt
     6301 ccaagaccta ttttagccat gtttgggaca ttcaaaccaa ctgtttttga aatgtgtccc
     6361 agtgtgtcag aagctccatc tggaacccct gcattgacaa agttattagc atctggtgct
     6421 gcaccgattc ctacagaatc cagtaccacc aaatgaatac gattaaattt agacatctta
     6481 tgcccccttc tattttcctt tttttgaagt tgaaatctga actccatctc gtccagcaac
     6541 gattacctta tccaccattt ggttgaataa accatgctcc acaacaccaa cgacatggtc
     6601 caattcttgt ccaaaagcaa ttggattttc aatgacatcc aaggcgaggt caatgataaa
     6661 attctgcata tccgtcacaa aacgttggcc gtctttttca cggaaacttg gtttgtagcc
     6721 agctcgttca aaatgacgaa agacctgctc tgcaccatac tgaaccactt ctactggcaa
     6781 tttaaaagca cctagttttt cgaccagctt gctttcatcc accacccaaa tgtattcttt
     6841 tgatggtgtt gcgaccacct tttccatgag aagggcacca ccaccgcctt tgattccatt
     6901 aaactgacta tccacttcat ccgcgccatc aaccgtcaca tcgacaaagt ctacttggtc
     6961 aatagacttg agcgggatat taaggccttc tgcctgttta gtggtcacac tagaagttgt
     7021 tacagctgta atctgcaatc cttcttcctt gatacgacga ccgatttctt cgacaaaata
     7081 ataggcagtc gagcctgttc caagtccaac gaccattcca tcgctcacga actcagcagc
     7141 cttgatacct gccattttct tcagattttc caccgattta cctccatcat tctcagttct
     7201 tattttacta tgaaaccgct ttttattcaa gtaaaaacat caaatctaca tttttcatca
     7261 atctttcttt aaaaatatgc attttatttt taccaaacga ataaaaatga gtttatcttt
     7321 tatgtatttg atttgttcat ctctttccag ttacaaaaac aaactcttaa ctttgatacg
     7381 tctagtttag ccttaaagca ggcgcatttc aagaatcata aattaagaga aggaagtaga
     7441 tttatctgtc tattttaaac aagtttatct ttgcttaccc tctcttgttg acaaataatt
     7501 ttcattattt tgggaagata cctgctgatt tccgtcggta agaccacata attttcctga
     7561 gatagttctt gggctatggc tgaatgaaga tgggtcgcta ctgccacacg ctcgtagaga
     7621 ctggcctgtc ggaattggcc tacaaatcct gcgatcatcc cagccagagt atctcccatt
     7681 cccccagtcg cctgataagg accaccaacc tgtaactggt aataatcaga ttggccaact
     7741 tcccaaatac gagtagctgg gcctttctcc accaaaattg ttccttgagg gaaggaagtc
     7801 agaacactag ccgttgcatc ttccttttgc ttttcaatcg taattccaga taatttttcc
     7861 cattcttttt ggtgaggagt taggataagc tggcttgagg gaaatgacaa acttgtccta
     7921 gcaagaatgg tcaaggcacc tccgtctaca atcaaaatct gattttggct taaattaaca
     7981 aagacctgtt ttactagatt ttctccagaa gcatcgtctc gtaaaccagg ccccaacaag
     8041 acaacttctg ccttctccaa ttgctctttt aacaattgct ggtcttgaag agaaaaggcc
     8101 ataggctcag gtaaatggct gtgcagagcc gggatatttt ccctgtccgt tccaacggtc
     8161 accaatcctg caccgctttt tacagctgct aaagcagcca tgatgatggc acctccataa
     8221 ggataagtac caccaagcag cagcagacga ccataatctc ctttatgact tgaacgagaa
     8281 cgttcaataa taactttttc taataaggtt tgattaatca ctttcatcct tttttcctct
     8341 cacttttatt atacaataaa aaggagacgc agacctcctt ttgtaatctt atatctaaaa
     8401 tttaatattc atttctgcca ttttagatat agctatagaa aatacactct attaatcgaa
     8461 tgtttctctt attttctatc caatgtccga agtgctgctt gataagtttg ctccatcagc
     8521 atggtaatgg tcataggacc gacacctcca gggactggcg tgatatagct agcaagtggt
     8581 gcaactgcct cataatcaac atctccacag agcttcccat tttcatctcg gttcatccca
     8641 acgtcaatga caaccgcacc tggtttgaca aagtcagcag tcacaaactt ggcgcggccg
     8701 attgcgacta caagaatatc tgctttagca gccaccttgg caagattatg agttcgtgag
     8761 tgggtcaagg ttactgtcgc atttttagcc aaaagaagct gagccatagg ttttccaacg
     8821 atatttgaac gaccgatgac gaccgcattt ttaccttcca agtcaatccc atattcatga
     8881 aacatttcca taattcctgc aggtgtcgag ggaatcatga ctggatgtcc agaccaaaga
     8941 cgtcccatgt ttaagggatg gaaaccatcc acatcctttt ctgggtcaat ggctaataaa
     9001 accgcctctt catcgatatg ttttggtaat ggcaactgga ccaaaatccc atgccaagct
     9061 ggatcctgat tatatttagc aatcaggtct aacaattcct cttgagtaat ggtctctgga
     9121 actcgcacta cttcgctacg ggaaccagcc gcaagagctg acctctcctt gttgcgaacg
     9181 tagacttggc tggctggatt atccccaacc aaaatcacta ccaaaccagg cactagagct
     9241 gtttcttcct ttaattttgc agtcttttca gccaactgcc cctgcaattt ggccgctaaa
     9301 gctttcccat caataatctg tgtcatatca cttctctttt ctttcaaata tcttccatta
     9361 taccaaaaac tgctagtgcc ctctaacact tctttcctag gttatcctat agtttcaaac
     9421 tataagaaaa agctctggtt gaacttttca ctaatcttgt cttgaaattt taaaatagat
     9481 tataaaacct tactcaagaa atctttagtc cgttgttctt gggtttgttc aaaaatctgc
     9541 tcaggtgtcc catcttcaac aaccacaccg tctgccataa agataacacg atctgccacc
     9601 tcacgggcaa atcccatctc atgtgttacg ataaccatag tcatccctga cttggcaagg
     9661 tcttgcataa cagccaatac ctcacctacc atttcaggat ccaaggctga agttggttcg
     9721 tcaaagagca aaacatctgg ttccatagcc aacccacgcg cgatggcaat ccgttgttgt
     9781 tgcccacccg acagactctg tggataagcg tctgccttat ctggcaaacc aactttttcc
     9841 aaaagctctt gggctctctt ctcagcaact ttcctgcttt cacctttagt cttgataggt
     9901 gacaaggtaa tattttccat cacagtcata ttaggaaaga ggttaaattg ttggaaaacc
     9961 atgcccatct tctcacgcat ggcaaacagg tcattcttct tgtccgtaat atcgactccc
    10021 tcaaagataa ccttcccctt ggttgcttcc tccaacaaat tcatagagcg aagcaaggta
    10081 gatttcccac tccctgaagg accgatgata acgacaactt ctcctctttt aatctcgagg
    10141 ttgatgccct tcaatacttc attctttcca aaagatttat gtaaattttc aatttttatc
    10201 aaggtttctg tcattatttc ttatctcctt gtcccatatg tttttcaaaa gctttcaagg
    10261 ctactgtcag aatagaggtc ataatcaagt agtaaaatgc tgcaaataaa agtggtgtta
    10321 aaggtagata ggttgttgta gaaactgttg tagccccatt ccacaactcc atgaccccaa
    10381 tagctgataa gagggagctg tccttgataa tggtgataaa ttcgttcccc aatgctggca
    10441 agatattttt gactgcttgt ggcaaaatca cataacgcat cgcattttta ggacgaatcc
    10501 ctagcgaata agccgcctct agctgacctt ttggaaccgc attgattccg gcacgaacag
    10561 tctccgaaac ataagcacca ctattcatag agataatcaa aatccctgga atcagacgcg
    10621 aaaaatcaac acctaaaatt ccaatctgaa tagtcggagc attgatatgc ataagagcaa
    10681 aggcaatcat aatttgaacc atcatcggtg tcccacggaa aatccaaacg tacaagttgg
    10741 ccaaccaaac aagcggttta aactttgaac gttgcccaaa agccaagaca acacccaaaa
    10801 tagttcccaa aaagataaca cagatagaaa taagaatcgt cacaacagcc ccatagttaa
    10861 aataaggtaa atacttaggt aaaaaagaaa aattcatagt cacactgctt tctaaaatag
    10921 aatactgtat gattataaca tgttttgaat taaaatttaa attttttatc caatttattt
    10981 aaaaatcttt aaactagcag aattaacgag aaatcttagt tttttctagt caagttggcc
    11041 tcctttatgg taaaatagta acgataagaa atgaaggaga atcaaaatgg aatcacattt
    11101 ggttagaatc atcaaccgcc ttgaagcaat ggcaaacgat ggcggaaatt taaaacgtaa
    11161 ttttgaacgc gaaggagttg ttgttgcaga agttgcgtac agccacgacg aagaaaacgg
    11221 cccaatcttt acccttcgtg acgtagaaga tcgcgaaacg tatacgtttg atagcattga
    11281 cttaatcgca atggaaattt acgaacttct ctactaatct aaaacaagct gggattgtcc
    11341 ctgcatgtct aagtaaaatc ctttttgtct cacaaatagg atttttttat agtccaaatt
    11401 cacaaaaatt ttcattgtaa caacttctca aagcttcaat ctttttactt gctttacacc
    11461 ccaatcctgt tataatgaga gtatagaatt tgactatttt tgacctttaa acaagtcagg
    11521 ctaaagaaag aaatgaggta gatgtatgct ttgtcaaaac tgtaaaatta acgactcaac
    11581 aattcatctt tacaccaatc tcaatggaaa acaaaaacaa attgacctct gtcaaaactg
    11641 ctataagatt atcaaaacag atcctaacaa tagcctcttc aaaggtatga cggatctgaa
    11701 caatcgtgac ttcgatccct ttggtgattt cttcaatgat ctaaacaatt tcagaccttc
    11761 tagcaatact cctcctattc ccccaaccca atcaggtgga ggttacggtg gaaacggcgg
    11821 ttatggttcc caaaatcgtg gatctgctca aactccgcca cctagccaag aaaaaggcct
    11881 gctggaagaa tttggtatta atgtaactga aattgcccgt cgtggagaca ttgaccccgt
    11941 tattgggcgc gacgatgaga ttatccgtgt catcgagatt ctcaatcgta gaaccaagaa
    12001 taatcctgtc cttatcggtg aacctggtgt cggaaaaacg gccgttgtcg aaggtctagc
    12061 tcagaaaatt gtcgatggcg atgtgccaca taaactccaa ggtaaacaag tcatccgtct
    12121 ggatgtggtt agcttagttc aaggaacggg gattcgagga caatttgaag aacgcatgca
    12181 aaaactcatg gaagaaattc gcaaacgtga agacatcatc ctctttatcg atgaaatcca
    12241 tgaaattgtt ggtgctggtt ctgcgagtga tggtaatatg gacgcaggaa atatcctcaa
    12301 gccagccctt gctcgtggag aactgcaact agtcggtgct actaccctca atgaataccg
    12361 tatcattgaa aaggatgctg ccctcgagcg tcgtatgcag cctgttaaag tcgatgaacc
    12421 aacggtggat gaaacaatca ctattctcaa agggattcaa aagaaatacg aagattacca
    12481 ccacgttcaa tataccgatg ctgcgattga agcagctgca actctttcca atcgctacat
    12541 ccaagatcgc ttcttgcctg acaaggccat tgacctccta gatgaagctg gttctaagat
    12601 gaacttgacc ttgaattttg tggatcctaa agtaattgat cagcgcttga ttgaggctga
    12661 aaatctcaag tctcaagcta cacgagaaga agattttgag aaggcggcct acttccgcga
    12721 ccagattgcc aagtataagg aaatgcaaaa gaaaaagatc acagaccagg atactcctat
    12781 catcagcgag aaaactattg agcacattat cgagcagaaa accaatatcc ctgttggtga
    12841 tttgaaagag aaagaacaat ctcaactcat ccatctagcc gaagatctca agtctcatgt
    12901 tattggccaa gatgatgcag tcgataagat tgccaaggct attcgccgta atcgtgtcgg
    12961 acttggtacc cctaaccgcc caatcggaag cttcctcttc gttgggccaa ctggtgtcgg
    13021 caagacagaa ctttccaaac aactggctat cgaacttttt ggttctgctg atagtatgat
    13081 tcgctttgat atgagtgaat acatggaaaa acatagtgtg gctaagttgg tcggcgctcc
    13141 tccaggttat gttggctatg atgaggctgg tcaattaact gaaaaagttc gccacaatcc
    13201 atattctctc atccttctcg atgaagtgga aaaagctcac ccagatgtta tgcacatgtt
    13261 tcttcaagtc ttggacgatg gtcgtttgac agacgggcaa ggacgcaccg ttagcttcaa
    13321 ggatgctatc attatcatga cctcaaatgc aggtacagga aagaccgaag ctagcgttgg
    13381 atttggtgct gctagagaag gacgtaccaa ttctgtcctc ggtgaactcg gtaacttctt
    13441 tagcccagag tttatgaacc gttttgatgg cattatcgaa tttaaggctc tcagcaagga
    13501 taacctcctt cagattgtcg agctcatgct agcagatgtt aacaagcgcc tctctagcaa
    13561 caacattcgt ttggatgtaa ctgataaggt caaggaaaag ttggttgacc taggttatga
    13621 tccaaaaatg ggagcacgcc cacttcgtcg gactattcaa gactatattg aggacacaat
    13681 cactgactac taccttgaaa atccaagcga aaaagatctc aaagcagtta tgactagcaa
    13741 gggaaacatt cagattaaat ctgccaaaaa aactgaagtt aaaagttctg aaaaagaaaa
    13801 ataaatccta taaaaaaaga gtagaaaatg aaatttttct gcttcttttt ttactaaaat
    13861 aactgtaatt tcttgacagc ttgccctttg tccattatga
//
LOCUS       DAVKQB010000052        13335 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKQB010000052 DAVKQB010000000
VERSION     DAVKQB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13335
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.052"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            188..1180
                     /locus_tag="VW528_001717"
                     /pseudo
     CDS             188..1180
                     /locus_tag="VW528_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1182..1999
                     /locus_tag="VW528_001718"
                     /pseudo
     CDS             1182..1999
                     /locus_tag="VW528_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
     gene            2001..2786
                     /locus_tag="VW528_001719"
     CDS             2001..2786
                     /locus_tag="VW528_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9912511.1"
                     /translation="MKKYQRMHLIFIRQYLKQIMEYKADFLVGVVGVFLTQGLNMLFL
                     NILFQHIPLLDGWSFHQVAFIYGFSLIPKGIDHLFFDNLWALGQHLIRKGEFDKYLTR
                     PISPLFHILVETFQIDALGELLVGVLLLLMTITSLTWTWAKVFLFLISIPFATLIYTS
                     LKIVTASIAFWTKQSGAIIYIFYMFNDFAKYPIAIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLKDKDGLFNIGGLILISLIFFTLSLKLWNKGLDAYESAGS"
     gene            complement(3165..4360)
                     /locus_tag="VW528_001720"
                     /pseudo
     CDS             complement(3165..4360)
                     /locus_tag="VW528_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4862..5065
                     /locus_tag="VW528_001721"
     CDS             4862..5065
                     /locus_tag="VW528_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9912512.1"
                     /translation="MYIRLKNLREDKDLSQCQIAQYLHCSQSAYSRIESGKQDIPTSF
                     LKQLAKLYQVTTDYILEMDKFSC"
     gene            5196..5348
                     /locus_tag="VW528_001722"
     CDS             5196..5348
                     /locus_tag="VW528_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912513.1"
                     /translation="MTTNKDNDTSERSGCQRKRTAGEMPMNDLTSHTHGGNYTCYKEE
                     KVWNNQ"
     gene            5515..7308
                     /locus_tag="VW528_001723"
     CDS             5515..7308
                     /locus_tag="VW528_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9912514.1"
                     /translation="MKKNNVEIIKADSLVRRRGDNVERHLKRVAAYCRVSSDSEDQKN
                     SYDSQVRHYKEYISQRSDWELADIYADEGISGTQVGKRQDFQRLINDCANGEIDYIVT
                     KAIARFARNTLDTLKYVRMLKDMQIGVYFEEENIDTLTMDGELLLTILSSVAQQEVEN
                     TSAHVKKGLKMKMQRGELVGFQGCLGYDYDVETKQISINKKEAKIVRYIFERYLEGIG
                     GKVIARELDELGYKSPRGLEHWNDTTVLGIIKNEKYKGDILMGKTFTVDPISKRRLSN
                     FGEEDKYYIKDNHEPIISKEDFEKAQEIRLRRAGNKKTAANVNGKRERYSKMYAFSSM
                     LECGFCGSILSRRSWHCRSDYRKVVWHCVTSIKKGKKFCKHSKGLEEIAIEGAFLEAY
                     RQVYHSNENLMTDLLETIESELNDNSLNKELKRITNKLRILLKKEENLVNLRLEGKVS
                     DSIYNEKYNEISSEKEFLAEEKVNIETTLKSEIDVKKRLTEFKHLLSSQKMLTEFDRA
                     VFESIVEKIIVGGVNSNGEIDPAMLTIIFKTGEIQNKDGKQFKSKRKNAKLETDKLCP
                     QNSDEDKKLYSQGTDNTRGVCSVAGSILASQ"
     gene            7413..8000
                     /locus_tag="VW528_001724"
     CDS             7413..8000
                     /locus_tag="VW528_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEU9912515.1"
                     /translation="MGGNPPIKKYSIVDKIVLSTKIKRIIIFTVFRENWEPYMKKYTE
                     VFQSQFPNLNIDYLLLDTEQIDLDSYLDADIIIIGGGNTEKYIATYINQEFKSYIDHM
                     LNKEAKIIGFSAGPLLLGEKVYVSPNDNSDHQIKIKNGLGLFSQFLISVHYDSWNDKA
                     NKDRAEELDNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            8282..8530
                     /locus_tag="VW528_001725"
     CDS             8282..8530
                     /locus_tag="VW528_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEU9912516.1"
                     /translation="MCKPISIELCDDEVHSLHEWIDGRDAIDSILTYSENQQYTYGVE
                     AGKILRKIHTIPATEVCEDWEIFFNLKIDDKISNEMIW"
     gene            8706..8959
                     /locus_tag="VW528_001726"
                     /pseudo
     CDS             8706..8959
                     /locus_tag="VW528_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9087..10316
                     /locus_tag="VW528_001727"
     CDS             9087..10316
                     /locus_tag="VW528_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9912517.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLMMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            10336..13032
                     /gene="ppc"
                     /locus_tag="VW528_001728"
     CDS             10336..13032
                     /gene="ppc"
                     /locus_tag="VW528_001728"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9912518.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    13115..13332
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         4259 a         2099 c         2685 g         4292 t
ORIGIN      
        1 atatgttctt cgtcataatt ctttgtactt gagtaattta gcttacttac agataaaata
       61 caagatggca tagttttagt agcttttttc atatggtttg taatgatggt aaattttctt
      121 aactttactg tagtatgcta caatttttgt tgatattata acttgagtga aacgaaagga
      181 gtgatttata aaaatgataa cagtagagca tcttcagaaa aattttgtga aaacaataaa
      241 ggaacctggc ttaaagggag cttttcgctc tttttttcat cccaagagac aagtctttga
      301 agctgttaaa gatttatcat ttgaggtgcc caaaggacaa attttaggct ttattggcgc
      361 aaatggagcg gggaaatcaa caactatcaa gatgttgacg gggattttga aaccaacatc
      421 aggtttttgt cgtatcaatg gaaagattcc tcaagagaac cgtcaaggct atgttaaaga
      481 tattggtgta gtttttgggc agcgaactca actttggtgg gatttggctt tgtaggagac
      541 ttactcggtt ttaaaggaaa tttatgatgt tcctgatgcg gtctttcaaa aacgcatgga
      601 ttttctaaat gaagttttag atttgaagga atttattcaa gatcctgtca gaactctatc
      661 tctaggtcaa cgtatgaggg cggatattgc ggcctcctta ttgcataatc cgaaagttct
      721 tttcctagat gagcctacta ttggtttaga tgtttcggtc aaggataata ttcgacgtgc
      781 cattacacag attaatcaag aagaagagac gaccatactt ttaacgaccc acgatttagg
      841 tgatattgaa cagttatgtg atcgtatttt tatgattgat aagggccgag aaatctttga
      901 tggaactgtg aatcaactga aaaagacttt cgggaagatg aagactctct cttttgaact
      961 acatcctggg caggattata ttgtctctca ttttgaagga ctttctgata tctatgtgac
     1021 gagacaggaa ttgtcacttg atattcagta cgacagctcg cagtatcaaa cggctgacat
     1081 tattcaacaa actttgtctg attttacgat ccgtgattta aagatgacgg atgcgaatat
     1141 tgaagatatt attcgtcgct tctatcgaaa ggagctttag gatgactaaa ttatggaaac
     1201 gctataagcc ttttgttagc gctggaatcc aagaattaat cacgtatcgt gtcaattttt
     1261 ttctttatcg cattggggat gtgatggggg cctttgtagc attttacttg tggaaagctg
     1321 tgtttgattc atctcaccag tctctcattc aaggttttac tttatctgat atgaccttat
     1381 atatcatcat gagttttgtg acgaatctac tgacaaaatc agatagttct tttatgattg
     1441 gatgggaagt aaaggatggc tctattatta tgcgactgct tcgtccagtt cattttgcaa
     1501 tgtcttatct tttcacagaa attgggtcta gatggctagt ttttgttagt gttggtctac
     1561 catttgtaat tctgattgca ggtctaaagc tcttatcagg tgagtccttc ttacaaattg
     1621 ttttaataac cacagtttac cttcttagct taatcttagc ttttttgatt aatttttttt
     1681 caatatttgc tttggttttt cagcttttgt gtttaaaaac ttatggggat caaatctttt
     1741 gaaaaattct ttagttgcct ttttatctgg aagtttggtt cctctgacat tttttccaaa
     1801 aattattgcc gaactattat cttttttgcc attttcttct ttaatctata ccccggtgat
     1861 ggttattata gaaaagtata gcatgagtca aatgattcag gcgctctcgc tacaattatt
     1921 ctggctgttt attatgattg ccttatccca actgatttgg aagtgcgtac agaattatat
     1981 aactattcaa ggaggttaga atgaagaaat atcaacgcat gcacctgatt tttatccgac
     2041 aatatcttaa acagattatg gagtataagg ctgatttttt agttggtgtt gtgggtgtct
     2101 ttttgacaca agggttaaac atgctgtttc taaatatttt gtttcaacat attcccttac
     2161 ttgatggctg gagttttcat caggttgctt ttatttatgg tttttctctc attcctaagg
     2221 gaattgacca tctctttttt gataatctgt gggctttagg acaacattta attcgtaaag
     2281 gagaatttga taagtatttg actagaccca ttagcccttt gtttcatatt ttagtagaaa
     2341 cgtttcaaat tgatgctctt ggagaattgt tagtaggcgt cttattgctg ttaatgacaa
     2401 taactagtct tacttggact tgggcaaagg tttttctctt tttaatcagt attccctttg
     2461 caaccttaat ttatacctct ttaaaaattg taacagctag tattgctttt tggactaaac
     2521 aatctggggc aataatttat attttttaca tgtttaatga ttttgcaaaa tacccgattg
     2581 cgatttacca ttcgttttta cgttggttga ttagttttat cattcctttt gcttttacag
     2641 cctattaccc agctagctat ttcttgaaag acaaggatgg attgttcaat attggtggat
     2701 tgatccttat ctcattaata ttttttacct tatcattaaa attatggaac aaaggtttgg
     2761 atgcatatga gagcgcaggg tcttagaaga agtaaaatct aattaagaat tttgatagaa
     2821 cttcctattc tttggaagga agttttaatg atacattgag agctttgatt aggcttactg
     2881 tagtttttca aacaagaaat ttgaccattg tgcaaaaatg atacaaaggt atgtaatatt
     2941 tcagtcataa ccactcgttc atatcatgag tggtttttta gctcttttat acataccatt
     3001 tgttaaggga catacgatat tctagctcaa aaggttttct gaatgagtat caaaggaatg
     3061 tttagaagat aattgcgagc tgttttttga aatttgggca cacttaaaag acacttatct
     3121 ggtattaaaa aataaggtgt tacaatgata ggacatagat gactttaatg actttgggtt
     3181 aaagtgtagt cgtaaagttt gttatgcgtt atgaggtaat acattgtccg gatgaggcga
     3241 tgtatagaga caatcgtata tggcttggtt gaagtcgtct gcgattgtct ttttcgtttc
     3301 tcataaaagt ccgcgatatg acaaggattg gtatgactag ctgaagcaat attgtggacg
     3361 cacttataga gaatttttct cgcgtaagga tttccacgct tggtgatatg ttcttgagct
     3421 aggaaattgc cagattcata gtgtctgagg tcaatactga taaaggcatt gatttggttg
     3481 gcagaccgaa agcgacgaat gtctccaatt cgccaataat gcttgtagct gttgtttctg
     3541 cgataccagg aatagagagc agaatctcgt attcaggtaa tggttcagcc aattcaatca
     3601 tttgtgctaa gtcctgatcc ttgcggtttg tcagtctcaa cagttcctgt gcatgatact
     3661 taacctcttc tgtcatggga gaggtttttg aaatggcaag ataggactgt tcggccagct
     3721 cgctcaactt ttcagccaat cgtccaactc tgttctcaga aatccgttta gaagttgaat
     3781 tacgatggct tcttttagct caactactga taatttcagc ctccaatgct tggttggaaa
     3841 gatggtcacg aggttccaat attgttcccc tgtaggagta gagagaaggt tctttatttc
     3901 tggaaaggca atttgaagca ccttgtgcag gctattctta gtcctaacga tgtcttctgt
     3961 cagattctga tagaatcgac tgaggtcacg caaattttga tagacttctt cttgaacata
     4021 agttggtttg cggttaagga tgaattgtga cgaagctagc ttttcagcgt caatctgatt
     4081 ggttttacgg actcgcaaac tgtctagttg cttcttagtt ttaaaggatt tagccgtata
     4141 taagcatagc tattgtcctc cagaaaggcc tgaagacgag aatagacccc tgtgcctcga
     4201 agataatctc aggtgtattt acctatttca aatcctctag aagacgacta aatccgatgg
     4261 catcattggg catggtatag ccatgaacct tttcaccatt cacaagaata gcgacttctg
     4321 agcttgcctt actcacatcc attctgaaaa ctgtccgcat catgtttacc cctttgtctt
     4381 gaatgactcc ttgttttagt gattttattt ccaatactcg acgtctagta tcccacatac
     4441 tttgataaaa ttcttcctaa aacaggtttc ttgccagttt gtcaagcgac gtctagcacc
     4501 atagagagct ctatgactta acaagacacc actattttaa cacaaagaaa aagtaatgag
     4561 tactctctcc cgttggagat ttcctcacta ctaatcttag tatgtttgtc aactgtagtg
     4621 atagacgaat aatcaagttc tggagaaggt cagattgggc gattacttat tattttccta
     4681 gcttgtacgg tgcttttatc gctatgaaaa agtttattcc aaaatctttt tttagtaact
     4741 tatagcttac ttactgttat accaactatt tctaaaatct tataaattat tttatgcata
     4801 ttttgcataa aattccaaat ttgataatct tcaaattaca atattagtaa gaggattatt
     4861 aatgtatatc agattaaaga atttaagaga agacaaagat ttgtcccagt gtcaaattgc
     4921 acagtatctt cattgttcac agtctgctta ttctagaata gaaagtggca agcaagatat
     4981 tcctacatca tttttgaaac aattagctaa attatatcag gtaactacag attatatttt
     5041 agagatggat aagtttagtt gttaataaat cctaagccct ttgaaaactg aataaaccga
     5101 ggtgggactt tcttatttgt cgagcacttt agcttgatac gccgtgatag gagcgataat
     5161 tattagctat aaaatagagt ggttctctat ctgccatgac tacaaataag gataatgata
     5221 cttctgaacg ttcaggctgc cagcgaaaaa ggacagcggg tgaaatgccc atgaatgatt
     5281 taaccagtca tacccacgga ggaaattata catgttacaa ggaggaaaaa gtttggaaca
     5341 atcagtaata aagtatacta tgcaactatc aatgcttaac tttttatact caagaaatat
     5401 gttatctaaa aatgagtatg acaagattag aaacaaactg cgtattaagt acacaaaata
     5461 gctgactttt tgatttgaat gcggtaatat agaatcaaaa ggaggaattt cagtatgaaa
     5521 aaaaataatg ttgaaataat aaaagctgat tctttagttc gtcgaagagg cgacaatgta
     5581 gaaagacact taaagagggt tgctgcttat tgcagagtta gttccgatag tgaggatcag
     5641 aaaaacagtt atgattctca agtaagacac tacaaagaat atatttctca gcgttcagat
     5701 tgggagctgg cagatattta tgcagatgaa ggtatttcag gaacacaagt aggaaagaga
     5761 caagattttc aacgattaat caatgattgt gcaaatggag agatagatta tattgtcaca
     5821 aaagcaattg ctagatttgc aaggaatacc ctagatactt tgaagtatgt cagaatgtta
     5881 aaggatatgc aaattggagt atattttgag gaagaaaaca tagataccct aacaatggat
     5941 ggagaattgc ttctaactat tctaagctcc gttgcacagc aagaagtaga gaatacttct
     6001 gcccatgtga aaaaaggatt aaaaatgaag atgcaacgtg gtgaattggt tggttttcaa
     6061 gggtgcttag ggtatgatta tgatgtagaa accaagcaga tttcaattaa taaaaaagaa
     6121 gccaaaattg ttcgttatat ttttgaaagg tatctagaag gtattggagg aaaagtaatt
     6181 gcaagggaac ttgatgaact tggttacaaa tcgccaagag gtttagaaca ctggaatgat
     6241 acaacagttc taggaataat aaagaatgaa aaatataaag gtgatattct aatgggaaaa
     6301 acttttacag ttgacccaat aagcaagaga cggttgagta attttggaga agaagataag
     6361 tactatatca aggataatca tgaaccaata atatctaaag aagattttga aaaagctcag
     6421 gagattagat tgcgtcgagc aggaaataag aagacagctg cgaatgtaaa tgggaagcgt
     6481 gaacgctatt caaaaatgta tgcttttagt agcatgttgg aatgtggttt ttgtggttca
     6541 atattatcta gaagaagttg gcactgccgt tcagattatc gcaaagttgt gtggcactgt
     6601 gttacatcga taaagaaagg aaagaaattt tgtaagcata gtaaaggatt agaagagatc
     6661 gccattgagg gggctttcct ggaagcttac agacaagtct atcattcaaa tgaaaattta
     6721 atgacagact tacttgaaac gatagaatct gaattgaatg acaatagcct taacaaggaa
     6781 ctaaaaagga ttacaaacaa acttcgaata ttactaaaaa aagaggagaa tcttgtaaat
     6841 ctaaggctcg aaggaaaagt tagcgattcc atatacaatg aaaagtacaa tgaaatttcc
     6901 tcagaaaaag agtttctagc agaagagaag gtaaatattg aaacaacatt aaaatcagaa
     6961 atcgatgtaa agaaaagact aactgagttt aagcacttgc tatcttcgca aaaaatgctt
     7021 acggagtttg atcgtgcagt ttttgaaagt attgttgaga aaattattgt aggtggagtt
     7081 aatagcaacg gagaaattga tcctgcaatg ttaaccataa tattcaaaac aggtgaaatt
     7141 caaaacaagg atggtaaaca atttaaaagt aaacgtaaaa atgctaaact agaaacagat
     7201 aaattgtgtc ctcaaaacag tgacgaggat aaaaaattgt attctcaagg aacagacaac
     7261 acacgtggag tgtgtagtgt tgctggttcg atcttagcca gtcaataaaa tgatatgttt
     7321 taaaaaaatt ataatttcga aacgtgaata aacttgttta aactttatgt atctttagaa
     7381 agtgtgttaa cttgaaagaa caaatttttt tgatgggtgg gaatccccca ataaagaaat
     7441 atagtattgt tgataaaatt gtattatcaa ccaagattaa aagaattatc atttttacag
     7501 tttttcgaga aaattgggaa ccgtacatga aaaagtacac ggaagttttt caaagtcaat
     7561 ttcctaatct aaacattgat tacttgctcc tggacactga gcagattgat cttgatagct
     7621 atctagatgc tgacataatt atcatcggtg gtggaaatac ggaaaaatat attgctactt
     7681 atattaatca ggagttcaaa agttatatcg atcatatgct taataaagag gcaaaaatta
     7741 taggattttc tgcaggaccc ctactattag gagaaaaagt ctatgtctca cctaatgata
     7801 attcagatca tcagataaag ataaaaaatg gattaggact ctttagtcag tttttaatta
     7861 gtgtccatta tgattcctgg aatgataaag caaataagga tagagctgaa gaactcgata
     7921 atgttcccat aattccacta aatgatcatt cttgtcttgt attggataaa cttggaaaca
     7981 tcatagagaa aattgactaa ctttcataat gtaaaaatgg atctaggaaa taaaagtgta
     8041 gacgaactgg ataggttact agtgaaaact gcaaaaagtg ttgtaaagtg aatcggagtg
     8101 tgcttgatgg attttagtag taagatagcc ataaataaag gctggtcaga tgataaaaaa
     8161 tattgtgtga cagatcaaaa tcagtaaaaa tatttcttgc gtgtttctga taagtcgaag
     8221 ttagattata aaaaaatttg aatttgatat gatggagaaa gtagcttctc ttggagttcc
     8281 gatgtgtaaa ccaattagca ttgaactctg cgatgacgaa gtacattctt tacacgaatg
     8341 gatagacgga agagatgcaa tagatagcat tttaacttat tcagaaaacc aacaatacac
     8401 atatggtgta gaagcaggaa agatacttag aaagattcat acaattcctg ctacagaagt
     8461 ttgtgaagat tgggaaatct tttttaatct aaaaattgat gataaaatct ccaacgaaat
     8521 gatatggtaa gtccgaaacg ctcagtgtgt aagcttgttg ctaaaagcgt aaaaaccttg
     8581 gaacgaaagg aataatagac ttcctgcgaa acaaaaatac aatacaataa aactatggat
     8641 gatgaagcaa gtaaacaatt aagcgatagc cgtttcaaga gccttgtagg tgttcagcgc
     8701 acgagatgtt agctgtgtta aaaacagctt atcaacgcaa cgtaaacgcg caaaaggtgg
     8761 acgaaaaacc aaattaagcc tagacgatct cctcatggta actattcaat acatgcgaga
     8821 atagcgcact tatgaacaaa ttgcggctga ttttggcatt cacgaaagca acttaatccg
     8881 tcggagccaa tgggttgaag taactcttgt tcaaaatggt tttacgattt caaattctgc
     8941 cctaattatg aaaaacagta aaattcgaag gattgtaagg taagagtttt tttctttctg
     9001 aaaaaatggt ataatagcaa tcaaaactag aaaataaaac ggaatttgga acagatttgt
     9061 ctgtatccta gtagagtggt gatgctatga agattagtaa gaggcactta ttaaattatt
     9121 ccatcttgat tccctacttg cttttatcta ttttgggctt gattgtggtc tattcgacca
     9181 ccagtgctat tttaattgaa gaaggcaaga gcgccttgca gttggttcga aaccaaggaa
     9241 tcttttggat tgttagtttg atactgattg ccttaattta taaattgaga ctagattttt
     9301 tgagaaatga gcgactaatc attttagtta tattaataga aatgctttta ttgttcttgg
     9361 ctcgttttat tggtatttcc gtaaacgggg catacggttg gatttcggtt gcaggaataa
     9421 ctattcagcc agctgagtac ttaaaaatca ttattatttg gtatttagct caccgattct
     9481 ccaaacagca agaagaaata gctacttatg attttcaagt tttgactcaa aatcaatggc
     9541 ttccccgtgc ttttaatgat tggcgattcg ttctcctagt tctgattgga agtttgggaa
     9601 ttttccctga tttaggaaat gcgactattt tagtcttggt ttccttgatg atgtatacag
     9661 ttagtggaat cgcttatcgc tggttttcaa ccattctggc gctcgtatct gccgcttctg
     9721 tctttgtctt gaccactatc agcctaatcg gtgttgagac cttttcaaaa attccagtat
     9781 ttggctatgt agccaagcgc tttagtgcct tttttaatcc ttttgccgat cgtgctgatg
     9841 caggtcacca gttagctaat tcttattttg ccatggtcaa tggcggttgg tttggtctag
     9901 gtcttggaaa ctcgattgaa aaacgaggtt atttgccaga agctcataca gactttgtct
     9961 tttctatcgt gattgaagaa tttggctttg ttggtgccag tcttatttta gctctcttgt
    10021 ttttcatgat tttgcggatt atcttggtcg gtatccgagc ggagaatcct ttcaatgcca
    10081 tggttgcact cggtgtcgga gggatgatgt tggttcaggt atttgtcaat atcggaggga
    10141 tttcgggctt gattccatct acaggagtga ctttcccctt cttatcccag ggtggaaata
    10201 gtcttctagt cttatcagtg gcagtagcct ttgtcttaaa tattgatgcc agtgaaaaac
    10261 gcgctaaatt gtaccgagaa ttggaaaatc aaccaatgaa ccttctgttg aagtaggata
    10321 aagaaaggat agtttatgtc tcttcaaaaa ttagaaaatt atagtaataa aagtgttgtg
    10381 caagaagaag tcttgattct aacagaatta ctggaagata ttactaaaaa tatgcttgcc
    10441 ccagagacct ttgaaaaaat aatacagttg aaagaattat caacgcagga agattatcaa
    10501 ggtctaaacc gtctagtgac tagcttatca aatgatgaaa tggtctatat ttcacgctat
    10561 ttctctatct tgcctctttt gattaatatt tcagaggatg tggatttagc ttatgaaatc
    10621 aatcatcaaa ataatattga tcaggactat ttaggtaaat tatctacaac gattaaattg
    10681 gtagcagaaa aggaaaatgc cgttgagatc ctagaacact tgaatgttgt ccctgttttg
    10741 acagcccatc caacacaagt gcaacgcaaa agtatgttgg atttaacaaa tcatattcat
    10801 agtcttttgc gtaaataccg tgatgttaag ttggggttga tcaataaaga taaatggtac
    10861 aatgatttgc gtcgttacat cgaaattatc atgcagacag acatgattcg tgagaaaaaa
    10921 ttaaaagtga ctaacgaaat cacgaatgct atggaatatt ataacagctc ctttttgaaa
    10981 gctgtacctc atttgacgac ggagtataag cgcttagcgc aagcgcatgg tctgaattta
    11041 aaacaggcta aaccaatcac catgggtatg tggataggtg gtgaccgtga tggaaatcca
    11101 tttgttacag caaagacctt gaagcagtct gcactcactc agtgtgaagt catcatgaac
    11161 tactatgata aaaagattta ccaactttat cgtgaatttt ctctttcaac tagcattgtc
    11221 aacgtcagca agcaagtcag agaaatggct cgtcaatcca aggataactc gatttaccgc
    11281 gaaaaagagc tttaccgtcg tgccttgttt gatattcaat caaaaattca ggcaactaaa
    11341 acctatctga ttgaggatga agaagttggg actcgttatg aaaccgccaa tgatttctac
    11401 aaggatttga ttgccattcg agattctcta ctagaaaata agggcgagtc cttgatttca
    11461 ggtgattttg tggaattatt gcaggcagta gagatatttg gtttttactt agcatcaatt
    11521 gatatgcgac aagactctag cgtctatgaa gcctgtgtgg cagaactctt gaaatcagca
    11581 ggaattcatt ctcgttatag cgagttgagc gaagaagaaa agtgtgacct tctcttgaaa
    11641 gaattagaag aagatccccg aattctttct gcgactcacg cagaaaaatc agaattatta
    11701 gcaaaagaac tagctatttt taagacggct cgtgttttga aagataagtt gggagatgat
    11761 gtcatccgtc agaccatcat ttcacatgca accagccttt ctgatatgct agaattagct
    11821 attctgttaa aagaagtagg actggtggat acggaaaggg cgcgtgttca gattgttccc
    11881 ctttttgaaa caattgaaga cttggatcat tcagaggaaa caatgagaaa atatctttct
    11941 cttagccttg ccaaaaaatg gattgactca cgaaataact atcaagaaat catgcttggc
    12001 tactctgaca gtaataaaga tggcggttac ttgtcatcat gttggaccct ctacaaggct
    12061 caacaacaat tgactgctat tggagatgaa tttggcgtta aggttacctt cttccatggt
    12121 cgtggtggta ctgtcggtcg tggtggtggg ccaacctatg aagccattac atctcaaccg
    12181 ctcaagtcta tcaaggatcg tatccgcttg acggagcagg gtgaagtaat tgggaataaa
    12241 tacggtaaca aagacgccgc ttactataac cttgaaatgc tagtatcggc agctattaac
    12301 cgtatgatta ctcagaagaa gagcgatacc aataccccaa atcgttatga agccattatg
    12361 gatcaagtag tggaccgtag ttacgatatc taccgtgatt tggtctttgg taatgagcat
    12421 ttctatgatt atttcttcga gtcaagtcca atcaaggcta tttcaagttt taatattggt
    12481 tctcgtccag ccgctcgtaa gactattact gaaatcggtg gattgcgtgc tatcccttgg
    12541 gtattctcat ggtcacagag tcgtgttatg ttccctggat ggtacggggt tggttcaagc
    12601 ttcaaggaat ttatcaataa aaatccagag aatattgcta tcttacgaga tatgtaccaa
    12661 aattggcctt tcttccaatc gcttctttca aatgttgata tggttttgtc aaaatcaaat
    12721 atgaatattg cttttgaata tgctaaactt tgtgaagacg agcaagttaa ggccatctat
    12781 gagactattt taaatgaatg gcaagttact aagaacgtta tcttggctat tgaaggacat
    12841 gacgaactct tagctgacaa tccatatcta aaagctagtc tggattaccg tatgccttac
    12901 tttaatattc tcaactatat tcagttagag ttgattaaac gccaacgtcg tggagaattg
    12961 tccagtgatc aagaacgatt gattcatatc accatcaacg gaattgcgac aggattgcgt
    13021 aattcaggtt gataattttc aagagtgaat gctaaaagtg aatatcaaaa aaattctaat
    13081 agactattga caagtagttt aaaaatgata taatttaacc attcagaaaa gtaatcatac
    13141 aaacttttta gagagtctgt ggtagctgaa aacagataag tggcaatgat gaaaattggg
    13201 ctgaatgcta tttagaattt gaaattataa aaattcggta agcacacctt acagtgcatc
    13261 tcgttattgc gagactgagc gatagggaaa ttccctataa ttgaggtggt accgcgcatc
    13321 gacgtcctca cacaa
//
LOCUS       DAVKQB010000053        12802 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKQB010000053 DAVKQB010000000
VERSION     DAVKQB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12802
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.053"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(118..1320)
                     /gene="rpsA"
                     /locus_tag="VW528_001729"
     CDS             complement(118..1320)
                     /gene="rpsA"
                     /locus_tag="VW528_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9912519.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRVARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1482..1553)
                     /locus_tag="VW528_001730"
     tRNA            complement(1482..1553)
                     /locus_tag="VW528_001730"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1519..1521),aa:Gln,seq:ttg)
     gene            complement(1563..1643)
                     /locus_tag="VW528_001731"
     tRNA            complement(1563..1643)
                     /locus_tag="VW528_001731"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1607..1609),aa:Tyr,seq:gta)
     gene            complement(1693..1923)
                     /locus_tag="VW528_001732"
     CDS             complement(1693..1923)
                     /locus_tag="VW528_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9912520.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2091..2735)
                     /gene="pcp"
                     /locus_tag="VW528_001733"
     CDS             complement(2091..2735)
                     /gene="pcp"
                     /locus_tag="VW528_001733"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9912521.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2750..3673)
                     /locus_tag="VW528_001734"
     CDS             complement(2750..3673)
                     /locus_tag="VW528_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9912522.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3670..4359)
                     /locus_tag="VW528_001735"
     CDS             complement(3670..4359)
                     /locus_tag="VW528_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9912523.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4424..5128)
                     /locus_tag="VW528_001736"
     CDS             complement(4424..5128)
                     /locus_tag="VW528_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9912524.1"
                     /translation="MVLSTLAILVACGKTDKEADAPTTFSYVYAVDPASLGYSITTRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPIVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            complement(5184..6206)
                     /locus_tag="VW528_001737"
     CDS             complement(5184..6206)
                     /locus_tag="VW528_001737"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9912525.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6336..8807)
                     /gene="parC"
                     /locus_tag="VW528_001738"
     CDS             complement(6336..8807)
                     /gene="parC"
                     /locus_tag="VW528_001738"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9912526.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLRIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKNFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEAILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(9227..11170)
                     /gene="parE"
                     /locus_tag="VW528_001739"
     CDS             complement(9227..11170)
                     /gene="parE"
                     /locus_tag="VW528_001739"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9912527.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTALKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            11306..11947
                     /gene="plsY"
                     /locus_tag="VW528_001740"
     CDS             11306..11947
                     /gene="plsY"
                     /locus_tag="VW528_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9912528.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(11990..12802)
                     /locus_tag="VW528_001741"
                     /pseudo
     CDS             complement(11990..12802)
                     /locus_tag="VW528_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3665 a         2869 c         2263 g         4005 t
ORIGIN      
        1 ttttttcaac ccactacagt tgacaaagag cccttttttt caacccacta cagttgacaa
       61 agagccagaa aaatcctttg tttagtaaac aaaggatttt gtgaattttc aatttgatta
      121 aagttcgata tcaccaaaca aatcagccat tgaaaatcct gtttgtgttt ctggaagttc
      181 gaaatcacgc ttttcttgac gtcttggacg acgtggacga gcaacacgtt tttcttcttt
      241 ttgtccttct tcttgggctg gacgttcttc aagagcttta atagaaagtg acacgcgttc
      301 tgcatctgcg ttaacttcaa gaactttaac ttgaacttct tgaccaactt taagagcttc
      361 ttttggattt tcaatccgtt tgtgtgaaat ttgtgatacg tgaacaagtc catcgatacc
      421 tggcaatact tcaacaaatg caccgaagtc agtcaaacgt ttaactgttc cttctactac
      481 atcaccttta gccaattttt gctcaacgcc atcccatggt cctggtactg ttgctttaag
      541 tgaaagtgat acacgtcctt cttcttcgtt aagatcaagg attttcactt caatttcttc
      601 accaacagtt acaactgatt ttggtgatac attacgttca tgtgacaatt cagtcaagtg
      661 aaccaatccg tcaacaccac caagatcgac gaaagcgccg aagcttgtga tacgagcaac
      721 tttaccagtt acaacatcac caacagccaa tttaccgaat acttcagcgc gagctgctgc
      781 agtagctgct tcaacaactt cacgacgtga aaggatgaag cggttttctt tagcgttaac
      841 ttctttgatt ttagtatcaa attcttgacc tacaaaacgc tcagcgttac gtacgaaacg
      901 agtatccaac attgaagctg ggataaatcc acgaacacct tcaaattcta ctgaaagtcc
      961 acctttaacg gcacgcgttc ctttaacagt aacaacttct tcttcgcgac caacaagttt
     1021 gtcccatgct ttgcgagctt caaggcgttt tttagataca aggtatgtaa ctgtatcagt
     1081 atctttacca actacttgac gaagtacaag aacatccaat acttctccta ctttaacaaa
     1141 gtcattgata tctgcatcac gatcgtttgt caattcgcga agagtcaaga caccttcaac
     1201 accagttcca gagattgcaa cgttagcttg agtcgcatca actgtcaata cttcagcact
     1261 aacaacatca ccagtctcaa cttgactaac gctatttagc aaatcttcaa attcattcat
     1321 ctaaaaaatc ctccaacaat caagtttttc ttaaacttga catacttata atttttttcc
     1381 taagcacccg caaggacatg ttcatatcat tcacgtcttt caattataaa aataaaatac
     1441 cctaagatat tttgcttttt atcttgatta ttacctaaga actggggtag ctggattcga
     1501 accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc ccattaatga
     1561 aatggagaga gagggattcg aacccccgaa cccgaaggag cggatttaca gtccgccgcg
     1621 tttagcctct tcgctatctc tcctacaatc aacatggact attatatcat gaaaatttca
     1681 aaaaaacaag acttattttg ctaaattata attttcaatc aaaatttcca cagctttttc
     1741 caatttttta taaaaactat cgacattccc attctttata atggcaaatt gtccgtctaa
     1801 ttcggcaatt tctccgataa cttttttacc gatagtcaat gtataacctt caaaactgtc
     1861 tttaccaaca ttaactttgg catccgctac ttgaatttcg atttttttat ctttcttgct
     1921 catttcatac ctctcttcac tttttctatt ttacaagaaa atcctaaaac tagcaagaaa
     1981 aaactctata tcaggcttct ctgatataga gttttatgta aacaacctca atcgatttag
     2041 catagttaat agttagtaaa caagcataat tgactatcct ttttaggtta tcagtgagtc
     2101 tcaccgccta ctaactgcaa atccttatct ttgtagtcta caatagcttc aatagctacc
     2161 tctatgcctc ttacaatatc acatagactc attcctgcag tattcggctt atttactact
     2221 tgttccatca tatatggaat atgcatgaaa cctgctctca tattagggaa ttttttatcc
     2281 actaaataaa gagcttggta catcaaatga ttgcaaacaa aggttcctgc actattggat
     2341 acaactgccg gaagtccctg ttttttgata gcttgtacca tcgctttgat aggtaaacta
     2401 ctaaaatagg ccgatgctcc atcaatacga atcggtgtat caattggttg attgccttcg
     2461 ttatcaggta tgcgagcatc atcttgatta atagccactc gttcaggtgt taagccggtc
     2521 ctgccgcctg cttgtccaat acaaagtaca gcatctggtt gatatcgtaa tatttctgcc
     2581 tctaaaactt ctgacgactt ataaaaaacc gttggaattt ctacccagcg aacttcagcc
     2641 ccattaatct cagatggtaa taattttaca gcctccaaag ctggattaat cttttcacct
     2701 ccaaaaggat taaaacctgt aaccaatatt ttcattttat tttcctttac taaaatgcga
     2761 gaaagtacat taagaatatg tgaataacaa tcattactag agcaacacct gcttgagcct
     2821 ttataacgcc attctgatct ttcatatcca tcaatgctgc tggtagagcg ttaaaattag
     2881 cagccattgg ggtcaataag gtcccacaat aacctgctgt catggcaaga gcaccagcca
     2941 caattggatt agctcccaga gcaaatacga agggaactcc aacacctgct gtaataacgg
     3001 tgaatgctgc aaaagcattt cccataatca ttgtgaatag aaccattcca agaacatagg
     3061 ccaaaactcc tataaagcga ctatctgaag gaacaatacc gctaatcaga tgagagataa
     3121 catcaccaac acctgctaca gtaaaaatag cccccaaagc ccctaataat tgaggaacaa
     3181 tcccacttgt tgaaacttgc tgagtcattc gattattttc tgataacaga ctcttagggt
     3241 gactattggt aatcacaaga acagaaattg tagcaaacaa ggcggcaagg ctaatcgaaa
     3301 tcttgctaaa ttctggaatc atttgcgcta agaccaacgc aagtattgcc atcagcataa
     3361 ctggaataaa aattttattt ttcaacctgt tagattcaat attggctttc atttcatcta
     3421 aggatggcaa ggttccgata cggacttgct taaacaatgt taacagcgat aataggatta
     3481 caataatacc aatactcata tttggcatat aggaaccacc tataaacgta atagacaata
     3541 gagtccaaaa tgcagatgtc ccaagtcgaa ctgggtttgt tttatcttta taactacaat
     3601 aggctgtatg gagaaattga caaccaatca caatataggt caactctaat agttgctttg
     3661 ccaactctgt catttttgtt ctcctcccct agtctttttt gatatcaatt ttttatcaaa
     3721 taaataattg taaatcccca ctacaataag tgttataaca gcaacaataa tagatgtaga
     3781 agcaatccct gcataattgc tttcatagcc taactgatct aatgttcccc ctatcaagag
     3841 gactccccca gcacctacaa acgtattttg agcaaagaaa tttccaaaat tttcattcgc
     3901 agccgcacgc gcttttattg tctcatcttc aacctctgtt aactttctac ctaattgaga
     3961 ctctgcagct gcttctccca taggttgaac caaaggtctg acaaactgag ggtgtcctcc
     4021 tagacgaatt gaaaagaaac cagctaactc tcgaataaag aaataaactg tatagaagtt
     4081 tccaactgtc agacctttaa tctttcgaat caaatcgatt gatcgttgct tgagtccaaa
     4141 ggtttctgac agccccacaa gaggcaaggt aaccataaaa atcgtgagca ctcgctgatt
     4201 gctaaattct tttcccaaaa tctccaaaaa ttcaacgaga gaaacacctg aaactaaagc
     4261 tgtaaccaaa ccagctaaga ctactgttgc aattgtatca aattttaaaa taaaacccac
     4321 aacaatgatt gctattccta ttaatctaat ccactccata tcaaactcct ttatattcaa
     4381 aatgacagta ttttaaaaat tttatcaaga tcaataccat tccttattta atgtgttttt
     4441 ctagttcttt ttggtatttg ctattggatt ccaatttttc tttttgccat tttttaaaaa
     4501 cctcgttata ttcttttgtt gtaacaatat ctttttgcaa tttcattcct ttaaagatat
     4561 atggttcccc cttaatacca acttgtgagt atggttttga gaatggtact acgttactta
     4621 caattggaga accaccagat gaagctgttg gcatcaataa tgaactatct gtcgaccaag
     4681 cttgagcttt ggcatatttt tcatatctct tctctaggtc agtggtctca gaaacagcat
     4741 cttctaacaa tttcttatat ttatccaaac caggtttagc tacaacatcc ttatcttttc
     4801 ctttcgtaat accaaggtgt ttcatggcag aaccagattt tggatccata atattcaagt
     4861 aagacgctgg atcttgatag cttggagccc atcctgtact attcaaatca tagtcttttt
     4921 gagaaggagc aacattgccg tatttatcat tttccatcaa accatcaata acatttccaa
     4981 taacgtctgt cgtcgatgtt cgagtcgtta tactgtagcc caatgatgct ggatctactg
     5041 catagacata agaaaatgtt gtcggtgcat ctgcttcttt atcagttttt ccacaagcca
     5101 ctaaaatagc taacgtgctc aggaccactc ctgctgttaa gagccacttt ttctatttca
     5161 taaaaaatct cctttggttt attttaatct acttttacaa tccaaccttc tggcgcttca
     5221 atatcgccaa actgaatacc cgtcaattca ttatataatt tacgcgtcac aggacctact
     5281 tctgtttcac tatagaatac atggaaatca tcaccatgtt gaatacctcc aattggagaa
     5341 ataaccgctg ctgtaccaca ggcacctgcc tctacaaaac ggtcaagatt atcaattgga
     5401 acatcaccct caataggagt taatcccaag cgatgttctg ccaaataaag caaggaatac
     5461 ttggtaatag atggcaagat agatggactc aatggtgtta caaattcatt atcagctgta
     5521 attccaaaga aattagctga tccgacttct tcaatctttg tatgagttga tgggtccaga
     5581 tagataacat ctgagaaatg gcgtgacttg gccatttttc ctggtaagag acttgcagca
     5641 tagtttccac caaccttagc cgcacctgta ccatttggtg ctgcacggtc gtactcatcc
     5701 tgaatcaaga agttggttgg gaccaaacca cctttaaagt aatttccaac tggcatagca
     5761 aagatggtga aaatgtactc ttctgccggt tttaccccga taatatctcc gacaccaatc
     5821 aaaagagggc gaagatataa ggttccacct attccgtatg gtggtacgta ttcttcattc
     5881 gcacggacaa ctgctttaca agcttctaca aacatgtctg tcggaacttg tggcatcaag
     5941 agacggtcac atgtacgttg caggcgttta gcattttcat caggacggaa cagttgaaca
     6001 ctgccatcct tagtacgata agctttcaaa ccttcaaatg cttgttgtcc atagtgaaga
     6061 cttggagaag actctgaaat atgcaaagtt gcatcctctg taagctctcc ttgatcccat
     6121 tgtccatttt tgaaatgagc aagatagcga taaggtaatt tcatatagga aaaaccgagg
     6181 ttttcccaat caatcgttac tgtcatattc cactccttat tctaaaaacc tatttcttat
     6241 attctacact atttttctaa aatagcaagt atattttgta attttcagaa aattcctcca
     6301 ataaaaacca tcttagaact gattcttcat ttcacttatt tatcttcagt aactacttcc
     6361 tgaagataag cgtcaaaaac ttcttcatct gaaatcgtgt cagaaatgaa gcttccattg
     6421 ctagtgcgtt ctgacaagtt caagtcttgc aatcggcttt catagattgt tcctttattg
     6481 gattggacaa gcagagtttg gtcgttcaca tccacttccg tactgaagaa atcgccaaca
     6541 aatccttgct ctgcaactgc tcctgccaag aagacacgat gcggtttgtt tttcaactca
     6601 cgcaagactt gtaatcctcg tttggcacgg ctggttgcta gaatttcctc aatggaaaca
     6661 cgtttcaagc ttccacgctg ggtcaagagg tagaaggacg aagtattaca gataaagcca
     6721 gattggagga catcatcttc tttcaaattc atagccttga cacctgctgc cttagcaccg
     6781 acaaccggaa cctcttcgat attgaaacgc agggcataac cattttgact aaccaagaca
     6841 acatcatcta gtttaatcgg agccactgct acaatctgat ctgtatcgtc tttgagctta
     6901 gcatacttga cagacttaga tctataggtc cgccatggag tgaattcttt tcgctctacc
     6961 cgtttgattt gaccaaggcg agtcgctgca aagtaggttg tcgcatcgtc aaactgatcc
     7021 agtacttcca cataaaggat tgcttcattc gtttcaaagt ttgtgatggt ttggctcaga
     7081 tgctctccga tgtccttcca acgaatatct gccaactcat ggattggtct gtagatgaca
     7141 tttccaagac ttgtgaacat caagaggtgc tgggttgtct tggcagattg aacaaaaatc
     7201 aaacggtcat catcacgctt gccaatttct tccaaggtgg aagccgcaaa ggaacgtgga
     7261 ctggtacgct tgatgtaacc tgccttggtc acgctgacgt aggtatcttc ctcagcgata
     7321 agactagctg tatcaatctc aattgctttc gcagtgtctt ctaaagaact caaacgagga
     7381 gttgcaaagt tcttcttgac ctcacgaagt tctttcttca tgagattgta catagtcctt
     7441 tcatcaccga taatagccgc cagcatagca atcttctcac gaagctctgc ttcttcttcc
     7501 tgcaagacaa ccacatcggt attggtcaaa cggtacagtt gcaaagttac gatagcctca
     7561 gcctgttctt ccgtaaaatc atagctaact ttgaggtttt ccttggcgtc cgccttattc
     7621 tcagaagcac ggataagagc aatgacttca tccaaaatcg aaatcacacg aatcaaacct
     7681 tcgacgatac ggagacgttt ctcagccttt tctttgtcaa agcgtgaacg cgccaaaatc
     7741 acttctcgac ggtgagcgat gtagctagac aggattggaa caatcccaac ctgacgaggt
     7801 gtgaaattgt caatcgccac catattaaag ttgtagttga tttgtaggtc ggtgtactta
     7861 aataagtagt tgagaacaag ctcagtatta gcgtctttct taagttcaat agcgatacga
     7921 agaccatcac ggtcagactc atcacgaacc tcagcaatcc cagctacctt gttattaaca
     7981 cgaacatcat cgattttctt gactagattg gccttattga tttcataagg aatctcagta
     8041 ataacgattt gttccttacc accttttagc ttttcaattt cagtcttgga acgaacaacc
     8101 acgcgccctt tcccagtctc ataggctttc ttgatttcat cacgaccctg aataatagcc
     8161 cctgtaggga agtctggtcc aggtaagaat tccatgagtt tatcaatctt tgcagttggg
     8221 tggtcaatca tgtaaactgc agcatctatg acctcagcta aattatgggg aggaatgtct
     8281 gtggcataac cagccgaaat cccagtcgaa ccattgacca agaggtttgg aaaggctgct
     8341 ggcaagaccg ttggttcttt ctccgtatcg tcaaagttcc atgcaaaagg aactgtcttt
     8401 ttctcgatat cctgaagaag gtagcctgca atttcagaca aacgtgcctc agtataacgc
     8461 atagccgcag gaggatctcc gtccatagaa ccgttattac cgtgcatttc aactagaatc
     8521 tcacgatttt tccagttctg tgacatacga accatggcat catagataga agaatccccg
     8581 tgtgggtgga aattccccat gatgttcccg actgacttgg ccgacttacg gtagctcttg
     8641 tcaaaagtat tgctatcctt attcatagaa taaagaatac ggcgttgaac cggcttcaac
     8701 ccatcacgaa tatctggcaa agcccggtct tgaataatgt acttggagta gcgaccaaag
     8761 cgctctccca tgatgtcctc cagggacatg ttttgaatgt tagacataag atacaaagcc
     8821 cataaaatac caagtgaaaa tagaaaattc ttgaagtaag caaactcaca agagaattta
     8881 tctttttcac acagtatcta gggcgtgttc aactcctttc aaagaatgta gagtaggttt
     8941 ttatgcagta aaagatattt tacgggaatt cgtcccgtgt tcagttacga taagtaacca
     9001 aactatcctg tttgtatttt tcaatatgaa aatctggttt tccaaaatta gtcttagttt
     9061 gtgtcttaac cgctccctta agcgcctctt taagataagc actcatagca gattcttcat
     9121 taataatcct gcaatttttt caaaccaaga ttttcaaact gctttttcac atagtcattc
     9181 acatccgact ctaatttcca gtttactaac atattatttt ctttcattaa aacactgtcg
     9241 cttcttctag cgtaaacttg acattatctt caatccattt acggcgtggt tctaccttat
     9301 ctcccatgag aacattgacg cggcgttcgg cgcgcgctaa atcttcaatt gtgacacgga
     9361 tgagggtacg tgtttctggg ttcatggttg tttcccagag ctggtccgca ttcatctcac
     9421 caagtccttt gtatcgttgg agggtagcgc ctttaccgaa ctgtttacgg agttcttcta
     9481 gttctccgtc cgtccaagcg taggccactt cttctttctt gcctttacct ttggacatct
     9541 tgtaaagagg tgggagggca atatagacat gacctgcctc gactagcgga cgcatgtaac
     9601 ggtagaaaaa tgtcaagagc aaggtctgga tatgggcacc gtcggtatcc gcatcggtca
     9661 tgataatgat cttatcatag ttggcatctt caatagagaa gtctgctcca acacccgcac
     9721 caatggtata aatcatggta ttgatctctt catttttgag gatatccgcc atcttggcct
     9781 tggctgtatt gataacctta ccacgaagag gtagaatagc ctggaacttg cggtcacgac
     9841 cttgtttggc agaaccaccg gcagagtccc cctcaactag atagagttca ttcttagcag
     9901 gattcttaga ttgggctggg gtcaatttcc cagacaacaa gcccttatct ttcttgtttt
     9961 tcttcccatt tcggctctca tcacgcgcct tacgtgctgc ttcacgagca tcacgggcct
    10021 tgatagcctt gcggatgagg ttagaagcta attccccatt ttccataagg aaaaaggtca
    10081 acttatcagc cactattcca tccacaactg ggcgagctag ggggcttcct agtttatcct
    10141 tggtctgtcc ttcaaactgc aagtgttctt caggaactaa gatagaaaga acggccgcta
    10201 gtccctcacg atagtctgaa ccttcaaggt ttttatcttt ttccttgaga agacctgttt
    10261 tacgtgcata gtcattcatg accttggtaa tggcagactt gagtcctgtc tcgtgcgttc
    10321 caccgtcctt ggtgcgaacg ttattgacaa aggatagaat gttatctgag aatccgtcat
    10381 tgtactggag ggctacttcc acttgaaaac cattgtcttc cccttcaaag taaagaactg
    10441 gcgtcaagat ttccttatct tcgttgagat aagaaacaaa atcttgtact ccattctcat
    10501 agtggaactc aatcgcttca tctgttcgct tgtccgttaa agacaaggtc acatttttca
    10561 agagaaaggc tgattcatta aggcgctctg aaatggtatt gtacttgaaa tctgtcgtag
    10621 aaaagatagt cgcgtcaggc ataaaagtaa ctttggtgcc tgttttagac ttgagtgctg
    10681 taccgatttt cttcaaagtc gtgacaggtt ttccaccatt ttcaaaacgt tgcttgtaaa
    10741 ctgcgccatc acgggtaatt tcaacttcta accagctaga aagggcgtta acaacggaag
    10801 aacccactcc gtgaagtcca cctgatgtct tatagccacc ttgaccgaat ttccctccgg
    10861 catgaagaat ggtaaagata acctcaacag ttggaattcc catagcgtgc atacctgtcg
    10921 gcatcccacg tccatggtct tgaaccgtta gactaccgtc tttattgata gttacatcaa
    10981 tacgatcacc aaacccagac aaggcttcat cgactgcatt atcaacgatt tcccaaacta
    11041 ggtgatgaag accagcgcca tcggtcgatc caatatacat ccctggacgt tttcggaccg
    11101 catccaaccc ttctagcacc tgaatagcat catcattata attgttaata ttgatttcct
    11161 tttttgacac aaggaacctc ctattcgttc atctttacta ttctacaggt tttccaagga
    11221 ttttgcaaaa tttttctttc tccgatgtga caatttcagc agagattctc tgcttttctt
    11281 tcccaattca tgatataata ggagtatgat tacaatagtt ttattaatcc tagcctatct
    11341 gctgggttcg attccatctg gtctctggat tggacaagta ttctttcaaa tcaatctacg
    11401 cgagcatggt tctggtaaca ctggaacgac caacaccttc cgcattttag gtaagaaagc
    11461 tggtatggca acctttgtga ttgacttttt caaaggaacc ctagcaacgc tgcttccgat
    11521 tatttttcat ctacaaggcg tttctcctct catctttgga cttttggctg ttatcggcca
    11581 taccttccct atctttgcag gatttaaagg tggtaaggct gtcgcaacca gtgctggagt
    11641 gattttcgga tttgcgccta tcttctgtct ctaccttgcg attatcttct ttggagctct
    11701 ctatcttggc agtatgattt cactgtctag tgtcacagca tcgatcgcgg ctgttatcgg
    11761 ggttctgctc tttccacttt ttggttttat cctgagtaac tatgactctc tcttcatcgc
    11821 tattatctta gcacttgcta gtttgattat cattcgtcat aaggacaata tagctcgtat
    11881 caaaaataaa actgaaaatt tggtcccttg gggattgaac ctaacccatc aagatcctaa
    11941 aaaataaaac gccagttctg tactgccccc aaacagttag acaaataatt tatccaaagg
    12001 atttagttct gtattgcaca gggctaagtc cttttagttt taccttaatt cgtttgttgt
    12061 tgtagtaatc aatatagtct ataatggctt gttccaattg gttaagtgat ttaaatgttt
    12121 tctcatagcc ataaaacatt tcggatttca gaatcccaaa gaaggactcc atcataccgt
    12181 tgtcttggct gtttcccttg cgtgacatag atgcttgaat tcccttactc tctaggaacc
    12241 gatgataaga atcgtgttga tattgccagc cttggtcact atggagaatc gtattctcgt
    12301 agtgcttctc tttgaatgcc tgttccaaca ttgtttgtac ttgttctaag ttgggtgaag
    12361 ttgaaagatt ataggcgata atttcgctat taaagccatc taaaactggt gataagtaaa
    12421 gcttttgagt acttactgga atggcaaatt cagtcacatc tgtgtagcac tgttccattg
    12481 ttttagagcc ttcaaattgg ccttgaatga gattctctgc tttcttgcca acgtctcctt
    12541 tatgagaaga atattttcgt ttctgtcgcg ttttagcttg taaattgagt acttcatcaa
    12601 gtgttgaact cttttatgat ttaccagata accacgattt cttagttcta aataaatccg
    12661 acgataagca taatttccct tgtgttcgat aaagatggat tgaatttcag ctttaagctc
    12721 ttggtcctta tctgttttgt ctagctgttt caagtgatag tagtaggtcg aacgagctag
    12781 tttaatggct tttagaagaa ga
//
LOCUS       DAVKQB010000054        12229 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKQB010000054 DAVKQB010000000
VERSION     DAVKQB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.054"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            122..277
                     /locus_tag="VW528_001742"
                     /pseudo
     CDS             122..277
                     /locus_tag="VW528_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="VW528_001743"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="VW528_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9912529.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGAEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYIDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERKNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3749..7399
                     /gene="addA"
                     /locus_tag="VW528_001744"
     CDS             3749..7399
                     /gene="addA"
                     /locus_tag="VW528_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9912530.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGCQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFCFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     HVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7614..8792
                     /locus_tag="VW528_001745"
     CDS             7614..8792
                     /locus_tag="VW528_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912531.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSIC
                     VCGNVIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKNGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDYRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            9001..>12229
                     /locus_tag="VW528_001746"
     CDS             9001..>12229
                     /locus_tag="VW528_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9912532.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIEIKIDFQEEI
                     QENPDLVEGIVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPIPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGVPEVQPALSKAVITDKG
                     ETEVQPESPDTVVSDKGEPAVQPELPEAVVTDKGETEVQPESPDTVVSDKGEPKQVAP
                     LPEYTGPQASAIVEPEQVAPLPEYTGVQAGSIVEPEKVEAPKEYTGKIEQPSAEDTKP
                     ENGASSTNGESERPKDKIKEEKQVDKKLELRNVSNVELYTVENNKYRHITAVDGALDS
                     SLKYFMKVKSENFKDIMLPVTKIESTTKNNKEVYKIVAHAENLIQHENNVISNDYTYY
                     LPKTQQSETGVYTSFKNLVDAMNSDPNGTFHLGATMDAREVELPDDQESYVKNEFYGK
                     LIGKNNGKYYAIYNLKKPLFKTLNTATIQNLSIKEANVSSKEDAATISKEAKYNTLID
                     NVHSDGIIAGERGIGGLVSKVDNSRISNSSFTGRITNTYDTTAGYEIGGLVGKLSGSL
                     ASIEKSIASIDIASNAKSGDQIVGGIAGVVEKSATIKYSYVEGNVNNVRHFGKVGGVA
                     GNLWDRDSQDVSKSGKLSYVLSDVNVTNGNAIAGYNFNGIKTIETYSNKNNKVVNVVQ
                     EDDEVVTKDSDVQRGTVLDADKVKEKKVELVSKHSTKVEDFDFTSRYNTNYNEVTGYQ
                     QSREQVYKNIEKLLPFYNRETIVKYGNLVEDNSDLFTKKLLSVVPM"
BASE COUNT         4030 a         2346 c         2552 g         3301 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagaaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct acccgtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagcctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cgaacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagctaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgtgttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 accatgagat gaccaaatct cagatgaatt ttttggactt ggagaattta acagatgagg
      901 ataagagggc ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaagggc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc gccttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgctga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcccgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatgtc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagcat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga cttgtatatt gacctcagtc
     1681 agtctgatat tgatgctttt gaacaatata tccgctatct tggtattaat ggcttgccag
     1741 cctttcagca aaccttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttttg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggaaaaacc taaaccaaac gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagga
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatactga gcaagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatcagca aggtctggaa aatccagcta
     2641 tcccaaccag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcat cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttacagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacgac tggacatatt ctccgacaca atccagccat cgaaaccatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggga gcagaacttt ttcggcgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggctaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcaggc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaagttgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggt
     3721 ttgaaaaaat aagagaggag ttaaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacagaaga aaactgccga
     3841 acaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg tagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgatctca aacaacactt
     4081 gggttgccag ttggcagacc tacccaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agagctttag tcatttgctg aaaaactttg ctgggcgtgg
     4321 caaggacgaa cggggtctgc gccagcaagt ctataaaatc tatgacttcc tccaatccac
     4381 cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa ggctttgaga aagctgattt
     4441 taccagtgaa aaagaaaaac tgactgagca aatcaaacaa gccctctggg atttggaaag
     4501 ctttttccgt taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga
     4561 aaatgttcag ttaattctgg atgaaattgg ctccctaaat caggagttcg atagtcaggc
     4621 ttatcaggca gtacttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgtgctct
     4681 gactaatgcc agccgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag
     4741 aaagacccag tttactaaac taggacaatt atcagaccag atagcgattc tcgactatca
     4801 agaacgttat catggagaca cttggaaact agctaaaacc ttccaatctt tcatgagcga
     4861 ttttgtagag gcttatcgtc agagaaaacg acaggaaaat gcctttgaat tcgctgatat
     4921 cagccattac accattgaga ttttagaaaa tttcccacaa gttcgtgagt cttatcagga
     4981 gcgcttccat gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat
     5041 gctggaattg ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat
     5101 ctatcgtttc cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca
     5161 aaatcctcaa gaaggcaagc tcattctcct caaggaaaat ttccgtagta gttcagaagt
     5221 gctgtcagca accaatgatg tctttgaacg tctcatggac caagaggtcg gcgaaatcaa
     5281 ctatgataac atgcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa
     5341 caaggctgaa tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac
     5401 agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca
     5461 agaaaaaggt gttgccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga
     5521 ccagattctc ctcgccctgt ctgagtacgg gattcctgtc aaaactgacg gagagcaaaa
     5581 caattatctc caatccctag aagtgcaagt catgctagac actcttcgtg tcattcacaa
     5641 tcccctgcaa gactacgcct tggttgccct tatgaagtct ccaatgtttt gttttgatga
     5701 ggatgagcta gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta
     5761 tgagaaactg gtcaatgcac aaaaaatggc aagtagtcaa aaaggcttga ttcacacagc
     5821 tctagctgaa aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa
     5881 accccactct ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta
     5941 tgttggggct ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg
     6001 tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga
     6061 ccaagtctta gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc
     6121 agtagagctc atgaccatcc acaagagtaa agggctggag tttccttacg tctttatcct
     6181 caatatggat caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca
     6241 gaatggtctt ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta
     6301 tcctaaaacc atcaaactct ccattcctag tctgacctat aggcagaacg aagaggaatt
     6361 acagctagca agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga
     6421 gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag ctggaatcca aggaataccc
     6481 agcagccaaa aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga
     6541 ttggctttgg gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt
     6601 tattggcgaa gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct
     6661 ccaagatagc tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctggaaat
     6721 gctgaaggag gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt
     6781 tcaaacccca agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga
     6841 gattgctggt caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc
     6901 aaccaaagaa aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagag
     6961 aattgacctc agccagcaac taacccttgc tagcctaaca gaaacactca aacacgttca
     7021 aactagccaa gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac
     7081 agtactcggt caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc
     7141 catgctcaaa cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg
     7201 ctatctgctt tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga
     7261 accaagtcaa ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc
     7321 acgagcctat tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt
     7381 tcaagttgta aaagtataac ttatactctt tgaaaatctc ttcaaaccac gtcagcttcg
     7441 ccttgctgta tatatggtta ctgacttcgt cagttctatc cacaacctca aaacagtgtt
     7501 ttgagctgac ttcgtcagtt ctatctacaa cctcaaaaca gtgttttgag ctgactgcgg
     7561 ctagctttct agtttgctct ttgattttta ttgagtatta gaaaggagac ctcatgacac
     7621 ttccagttag aaaatccctg cacgatgcag ttttacaggc ttcgaaagcg gatacttggg
     7681 aacaagctac caaggaatgg aatgaagttt ccttgatttt taacggtatc ggccgtagca
     7741 tttgtgtctg tggaaatgtc atcaaatacg cttatgaact ctttaacggt gtcacaggcc
     7801 aacgcctctt tccaattggt agcgactgtg ttcgtcattt tcatcgattg tcccttgacc
     7861 agcaattgga agaggaagaa aaactgctca gaaaggttga aaatctaacc agaaaagctc
     7921 agaaaaagga aaaaatcaag gtcaataaaa gtgactttga cgagcgactt ctaaaatggc
     7981 tctgggaaaa aggtgttttc aaacccaatc gtggcaatca atttgcgcct gagagagact
     8041 accagctttt cctagaagtc tttcagggag gaagttggac caaggcggaa ccaaagaaaa
     8101 aggctcggat ggaagaagtc cttgaaaagt gtatcaaacc ctttttactt gggaaatcgg
     8161 atgaccaact ctaccttgtc aagctaggca aggagaaaat tgactacgaa caagaactcc
     8221 gtatccaggc agagaaagaa cgcaagaaga gggataaaat cgccaagcaa tatgcagaca
     8281 atctggttct agccatggga cccgcagagc gtgcttatca agattacttt ggctttacag
     8341 agaccctgac tcaagaagaa cgcaagtggg aaaaaattct ttttggtaaa aatagagcag
     8401 aacgggctat taaggccaaa caataccaaa aagagctgga aaaagaccaa cgaattgcca
     8461 gtcaggatcc aattgaaaga aaacagaaac agacttggct cctcaattcc tattttcgtg
     8521 agcttcctga agaaaaagct cgattttcta ggctcttatt agaatatcga aaaaatggtg
     8581 aagtaccctt ttcaactgaa tacctgtcag atcatctcat cgactttttc tataaaatga
     8641 aagcctttga atttgaaatc gcaccagaac aagtccgaga ttttctaaaa aaaagcctgc
     8701 aggaagacta tcgctcatca gcacaaggaa gctggataga aggaattctc ctcaactgcc
     8761 tcaaaccatt tttagaacga ttagtgatat gagtaacacc taccgtgtaa atttcatggt
     8821 aggtgttatt gtacccccaa ataatataaa acaaagtatg aaagcgcatc tcaattttaa
     8881 gacttttttg taaattaaga gtataattaa ggtatactgc cttttctata gataatagat
     8941 aatacactaa tataataaag gtctttgtaa catatataaa ttttatttca aggaggaata
     9001 atggaaaagt attttggtga aaaacaagag cgtttttcat ttagaaaatt atcagtagga
     9061 cttgtatctg caacgatttc aagtttattt tttatgtctg tattagctag ttcatctgtg
     9121 gatgctcaag aaactgcggg agttcactat aaatatgtgg cagattcaga gctatcatca
     9181 gaagaaaaga agcagcttgt ctatgatatt ccgacatacg tggagaatga tgatgaaact
     9241 tattatcttg tttataagtt aaattctcaa aatcaactgg cggaattacc aaatactgga
     9301 agcaagaatg agaggcaagc cctagttgct ggtgctagct tagctgctct gggaatttta
     9361 atttttgctg tttccaagaa aaaggttaag aataaaacgg tattacattt agtattggtt
     9421 gcgggaatag gaaatggtgt cttagtttca gtccatgctt tagaaaatca tcttttgcta
     9481 aattacaata cggactatga attgacctct ggagaaaaat tacctcttcc taaagagatt
     9541 tcaggttaca cttatattgg atatatcaaa gagggaaaaa cgacttctga ttttgaagta
     9601 agtaatcaag aaaaatcagc agccactcct acaaaacaac aaaaggtgga ttataatgtt
     9661 acaccaaatt ttgtagacca tccatcaaca gtacaagcta ttcaggaaca aacacctgtt
     9721 tcttcaacta agccgacaga agttcaagta gttgaaaaac ctttctctac tgaattaatc
     9781 aatccaagaa aagaagagaa acaatcttca gattctcaag aacaattagc cgaacataag
     9841 aatctagaaa cgaagaaaga ggagaagatt tctccaaaag aaaagactgg ggtaaataca
     9901 ttaaatccac aggatgaagt tttatcaggt caattgaaca aacctgaact cttatatcgt
     9961 gaggaaacta tagagataaa aatagatttt caagaagaaa ttcaagaaaa tcctgattta
    10021 gttgaaggaa ttgtaagagt aaaacaagaa ggtaaattag gtaagaaagt tgaaatcgtc
    10081 agaatattct ctgtaaacaa ggaagaagtt tcgcgagaaa ttgtttcaac ttcaacgact
    10141 gcgcctattc caagaatagt cgaaaaaggt actaaaaaaa ctcaagttat aaaggaacaa
    10201 cctgagactg gtgtagaaca taaggacgta cagtctggag ctattgttga acccgcaatt
    10261 cagcctgagt tgcccgaagc tgtagtaagt gacaaaggcg taccagaagt tcaaccagcg
    10321 ttgtccaaag cagttataac cgacaaaggt gagactgagg ttcaaccaga gtcgccagat
    10381 actgtggtaa gtgataaagg tgaaccagca gtccaaccag agctaccgga agcggttgtg
    10441 actgacaaag gtgagactga ggttcaacca gagtcgccag atactgtggt aagtgataaa
    10501 ggtgaaccta aacaggtagc accgcttcca gaatatacag gaccacaagc cagcgctatt
    10561 gttgaaccag agcaggtagc accactacca gaatacacag gggtacaagc cggatcgata
    10621 gtcgagccag agaaagtaga agcaccgaaa gaatatactg gtaaaatcga acagcctagt
    10681 gcagaagata ctaaacctga aaatggggca agctctacaa atggggaatc ggaacgtcct
    10741 aaagataaaa ttaaagaaga aaaacaagtc gacaagaaac tagaacttag aaatgtttca
    10801 aacgtggaat tatatacggt agaaaataat aaatatcgac atattacggc tgtagatggt
    10861 gctttagata gttcattgaa gtatttcatg aaggtaaaat cagaaaattt taaagatata
    10921 atgttgcctg ttactaaaat tgaaagtaca acaaaaaaca ataaagaagt atacaaaatt
    10981 gtggctcatg cagaaaatct aattcaacac gaaaacaatg ttatttcgaa tgactataca
    11041 tattatttac caaaaactca gcaaagtgaa actggcgtct atacatcatt taaaaattta
    11101 gttgatgcaa tgaatagtga ccctaatgga acattccatt taggtgcaac tatggatgct
    11161 cgtgaagttg aacttccgga tgatcaagaa agttatgtaa aaaatgaatt ttacggaaaa
    11221 ttaataggta aaaataatgg taagtattat gctatttata acttgaaaaa acctttattt
    11281 aaaactctaa acaccgctac tattcagaat ctttcaataa aagaagcgaa tgtttcttct
    11341 aaagaagatg ctgctacaat atcgaaagaa gcaaaataca atactcttat agataatgtt
    11401 cattctgatg gtattatagc tggtgaacgt ggaattggtg gattggtttc aaaagtagat
    11461 aactcgagaa tttctaatag tagttttaca ggcagaataa ctaacacata tgatacaact
    11521 gcagggtatg aaattggtgg tttagtaggt aaactttctg gttcgcttgc ttctatagag
    11581 aaatcaatag catcgattga catagcctct aatgcaaaat caggagatca aattgttggt
    11641 ggtatagccg gagttgttga gaaaagtgcg acaattaagt atagctatgt tgaaggaaat
    11701 gttaacaatg tgcgacactt tggtaaagtt gggggagttg caggtaatct ttgggataga
    11761 gattcacaag atgtaagtaa atcaggtaaa ctatcatatg ttttaagtga tgtcaatgtt
    11821 acgaatggta atgctatagc tggctataat ttcaatggaa taaaaacgat tgaaacatat
    11881 agcaataaaa ataataaggt agtaaacgta gtacaagaag atgatgaggt tgtgactaaa
    11941 gattcagatg tacaaagagg tacggtatta gatgctgata aagtaaaaga gaaaaaggta
    12001 gaattggttt ctaaacacag cactaaagta gaagattttg attttacttc aagatacaat
    12061 actaattaca atgaagtaac aggttaccaa cagtcgagag aacaagtata taaaaatatt
    12121 gaaaaattac ttcctttcta taacagagaa acaatagtta agtatggtaa tttagtagag
    12181 gataacagtg acttatttac taaaaaactt ctatcagttg ttcctatga
//
LOCUS       DAVKQB010000055        12121 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKQB010000055 DAVKQB010000000
VERSION     DAVKQB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12121
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.055"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(447..697)
                     /locus_tag="VW528_001747"
                     /pseudo
     CDS             complement(447..697)
                     /locus_tag="VW528_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866636.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(690..806)
                     /locus_tag="VW528_001748"
     CDS             complement(690..806)
                     /locus_tag="VW528_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9912533.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(1021..1374)
                     /locus_tag="VW528_001749"
     CDS             complement(1021..1374)
                     /locus_tag="VW528_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9912534.1"
                     /translation="MTVRHPGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAQNVTIL
                     EGSNIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            complement(1492..2460)
                     /locus_tag="VW528_001750"
     CDS             complement(1492..2460)
                     /locus_tag="VW528_001750"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9912535.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(2605..3165)
                     /locus_tag="VW528_001751"
     CDS             complement(2605..3165)
                     /locus_tag="VW528_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU9912536.1"
                     /translation="MSNFLGSVHLLGVLFPDSTFLNAFESAIVAPLVEESLKLLPLVF
                     VLALIPVRKLKSLFLLGIASGLGFQMIEDIGYIHTDLPEGFDFTISRILERIISGIAS
                     HWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLALGTHFLFNSPFVELETELPLAI
                     PVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(3171..4522)
                     /locus_tag="VW528_001752"
     CDS             complement(join(3171..4081,4081..4522))
                     /locus_tag="VW528_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9912537.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNSMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
                     "
     gene            complement(4519..4863)
                     /locus_tag="VW528_001753"
     CDS             complement(4519..4863)
                     /locus_tag="VW528_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912538.1"
                     /translation="MYEEKQYFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFANTYIHDFMNCTPKV
                     RQKKSNFWGVLL"
     gene            complement(4884..5381)
                     /locus_tag="VW528_001754"
     CDS             complement(4884..5381)
                     /locus_tag="VW528_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9912539.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(5454..6815)
                     /gene="radA"
                     /locus_tag="VW528_001755"
     CDS             complement(5454..6815)
                     /gene="radA"
                     /locus_tag="VW528_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9912540.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYISGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(6829..7344)
                     /locus_tag="VW528_001756"
     CDS             complement(6829..7344)
                     /locus_tag="VW528_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9912541.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     TPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(7346..7789)
                     /locus_tag="VW528_001757"
     CDS             complement(7346..7789)
                     /locus_tag="VW528_001757"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9912542.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(7849..7942)
                     /gene="ffs"
                     /locus_tag="VW528_001758"
     ncRNA           complement(7849..7942)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW528_001758"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(7975..8442)
                     /locus_tag="VW528_001759"
     CDS             complement(7975..8442)
                     /locus_tag="VW528_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9912543.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(8643..9929)
                     /locus_tag="VW528_001760"
     CDS             complement(8643..9929)
                     /locus_tag="VW528_001760"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9912544.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(10160..10396)
                     /locus_tag="VW528_001761"
     CDS             complement(10160..10396)
                     /locus_tag="VW528_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9912545.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            10662..11473
                     /locus_tag="VW528_001762"
                     /pseudo
     CDS             10662..11473
                     /locus_tag="VW528_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11508..12121)
                     /locus_tag="VW528_001763"
                     /pseudo
     CDS             complement(11508..12121)
                     /locus_tag="VW528_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         3758 a         2806 c         1982 g         3575 t
ORIGIN      
        1 tgactgctaa aatatttcta taaattaatt tgactttcct gatagagttg tttcacatct
       61 tatttcaact cactatagaa ggaggaaaag gagggctggg tggctaactt cattatagaa
      121 ctttcaaaaa gatgaaaaaa tagcgtcaaa gaggcttttt tctctttaaa acaggaaatt
      181 tcaaagacaa tcttgcgact atccaaactg tacgaatagc gagacataac ttacttcgct
      241 aatactatag tagattgaaa ctagaatagt acacctcgat ttctaaaaca ttgttagaaa
      301 tcgatttgac tgtcctgaac gatttgtcct gttattattt cattttacta tatcttataa
      361 aaagatagaa atgtcaagca aaaatgacaa gtattttgat aataaacttg ccatcccatc
      421 aactttctac tatataaagt atgaactcat agcaaggact caaactcagc cacggtcatg
      481 cctaattgct cactcgcaac ctcaggtgtt agaagacctt gacgtactag atttactaaa
      541 cctactttta atccctcttc aaggccttct tctcgtcctt gttcacaacc ctctgcacga
      601 ccacgttcaa taccacgctc tagtccttgt tcaatacctt cgcatagtcc tgtgctaaca
      661 agacttgttc ttctcgcata tgtagttgac taaacatttt cctgtcctcc tcggaccagc
      721 tcttgtagtc cagcagttga tttgcttggg taatggctcg ttgcggttgc tgggtaaagg
      781 gcttgttgcc gaaaaactcc aaccatggct tgcgaacctt gtctttgctg gtttctctgt
      841 attttttaat tccaagaatg ccatcttaac cagatggttt tcctgtccat tgttggtaat
      901 cgccaatacc tcacctgttg tgtcttcgcg catactaacg ctatgaaaag ccaggccatc
      961 tgagaaataa ttgctatcca cgatagcaat gacgtaaaca ggagcgatgt gtttgtagat
     1021 ctagtgagta tcaccttctc gctgacgaat tttttcaaga ttttgattaa cctgactgca
     1081 caggtaagcc cacaagtgat tgatgaaaaa attctgatga tggacttgaa tctcaataat
     1141 tacttgagta ccgttatcca actccgccaa gacgtctata ctggtataaa aatcctgcac
     1201 cgagtaaggc atggagagta atacgtgaat attgctaccc tccaaaatgg tcacattttg
     1261 ggctggcaaa tccagcatat cgcgaataaa ttgacaagta atttctggat tgctaaagat
     1321 tttcttagct accaagtcat tggttgggct aatgcccgga tgacgtacag tcatatttct
     1381 tctcttttta ttatatcaca gttttaaatc tagctttact agattcaccg ctactatcta
     1441 tttattcgga aaaaagacga aaaaacctga gaatcatctc aggcttggtc attaaatttt
     1501 tttctcaata tcgaaaagtg gagaaagtgg tcgtttttca tgaatacgta cgatagcatc
     1561 ccctaggaga tgagcgattg aaatctgctc aatcttatca atcaaacgct cttctggcag
     1621 atagatggta tccaaaacaa ccaatttctt aatagctgat ttttggatat tgtccgtagc
     1681 aggaccagaa agaactgggt gcgtacagct tgcatagact tcaacagcac cagcttccgc
     1741 aagagcatct gccgcatgac aaatcgttcc agcggtatca atcatatcat caatcaagat
     1801 acaagtcttg ccttcaacct taccgatgat gttcataact tcactagtat tcatcttatc
     1861 aacgctacga cgtttatcaa taatagcgat agaagttttc aaaaattctg ccaacttacg
     1921 agcacgagtc acccctccat ggtccgggct gacaaccaca tagtcagaac caaccatacc
     1981 acgacgctca aaataatctg caatcagagg agcacccatc aaatgatcca caggaatatc
     2041 aaagaatcct tgaatttgcg cagcatgcaa gtcgatggtc aataaacgat ccactccagc
     2101 tacttcaagc atatttgcga caagttttga agtgattggc tcacgcgctc tcgcctttct
     2161 atcctgacgt gcatacccat agtaaggcat gacaacattg acagattctg cactcgcacg
     2221 cttcaaagca tctaccataa tcaaaatttc aagcagattg tcatttacag gcgaactagt
     2281 tgattgtagg ataaagacgt gtttcccacg gattgattct tcaatgttga cctgaatctc
     2341 tccatctgaa aattggcgaa cacttgattt ccccaactct atcccaatct cctgcgccac
     2401 acgttctgcc aattctttat tagaagaaag ggcaaatagc tttaaatcag aaaaagacat
     2461 gatttcctcc ggtatatatg tataacttgt gcttttcaca agattttcca tctaccattg
     2521 tagcgctttt tgcactattt ttcaatcaaa aataaaagaa gggcaccata tttgtaccct
     2581 tgcatcattc ttttgaaaaa tattctaggt catcaactca ttgtgtttct caacaaagca
     2641 ataagcatga taaaaaccat agagagcaat agccgtaacc actggaatcg ctaaaggcaa
     2701 ctctgtttcc aactccacaa aaggagagtt aaacaagaag tgagttccca aggctaaacc
     2761 tagaaaaata aggccctgtt tcttgccaac cttctgtcct ttataggctc tgtaaagcaa
     2821 gtaaacacct actacagcta gacctgaaaa agtccagtga gaggcaattc ctgagatgat
     2881 acgctctaaa attcgcgaaa tagtaaagtc aaagccctct ggcaaatccg tatgaatata
     2941 accaatatcc tcaatcattt ggaatcccaa accggaagca attccaagta aaaacaaaga
     3001 ttttaatttt cgcacaggaa tcaaagccaa aacaaaaaca agtggcaata atttcaagga
     3061 ttcttctacc aaaggagcca caatagcact ttcaaaggca tttaaaaatg tactatctgg
     3121 gaaaagaacc cccagtaaat gtactgaccc caaaaagtta gacaaataat ttatccaaag
     3181 gatttagttc tgtactgcac agaactaagt ccttttagtt ttaccttaat tcgtttgttg
     3241 ttgtagtaat caatgtagtc cacaatagct tgttcaaggt tttctaaaga tctaaagttc
     3301 ttctcatagc cataaaacat ttcggatttt aaaatgccaa agaaagattc catcatgctg
     3361 ttgtctgggc tgttgccctt gcgtgacatg gatgcttgaa ttcccttact ctctaggaac
     3421 cgatgataag aatcgtgttg gtattgccag ccttggtcac tatggagaat cgtattctca
     3481 tagtatttct ctgtaaaggc ctgttctaac atactcttca cttgttctaa gtttggcgaa
     3541 gtagaaagat gataagcaat gatttcactg ttaaaaccat ctaaaactgg tgataaatag
     3601 agtttctgtg tgctatttgg aatggcaaac tctgtcacat ctgtatagca cttttccatt
     3661 ggcctcgatg cttcgaactg gcgttgaatg agattctctg ctttcttgcc aatctctcct
     3721 tggtaggaag aatacttccg tttacggcga attcgagccg ttaaaccaag aattctcatc
     3781 aaccgttgaa ctttcttatg attgaccaca aaaccacgat tccttagttc taaatggatt
     3841 cgacgatagc catagttgcc tttatgttca ttatcaatgc cctgaatttc ggttttaatc
     3901 tctttatctt tatcaacccc atccagttgt ttcaactgat aatagtaagt cgagcgagct
     3961 aaacgtgctg tttcaagaag aaaatctagt cgaaatcctc ctgaaaccat ttctctaact
     4021 gtctctgcct ttctcgctct agggcttcgt ccctttcctc taactctttt aactttttta
     4081 ggtaggccac ctcagtccgt aagcgttcat tctcctcctg gagtcgttct agttctgtca
     4141 tttcttccca agtcttcttc cgtttacgtc ccattttagc tggtctccct cttgttttct
     4201 caacaatagt atacccgttt ttcttgtatt gtgccggcca aaatgaaagc attccttggt
     4261 ttgggagggc ataatcaaga ctaatacttc tttgtgaaca accttccagt aaggctttat
     4321 ccatcatttc ttgtttcaat ttagaagaat agtgacgatt cttccctttt ttaacgattt
     4381 ctatgccata acggtcaatt aatttcacca tgtactttag accagaaaca tccacaccaa
     4441 atctttttga aagctgtttg aagctttgtc cttgctttct tagttcatag atctgaactt
     4501 tatcttcata actcaatttc ataataaaac accccaaaag ttagattttt tctgtctaac
     4561 ttttggggtg cagttcataa aatcatggat ataagtatta gcaaaactag acaaccagcc
     4621 tgaaaggaac atccctccca ataaagacag aatcaaaacc ttctttggca attcccattt
     4681 tttcccaata cggaagagaa aataaagagc cggaatcatg taaaagagag ctagaaagat
     4741 agaaactccc attagtccat attccgcacc tgacctcgaa ccgtccgtat agtagatggt
     4801 ttcatactgt aaaccaatac atagcaataa aataaaaata aaatactgct tttcttcata
     4861 cactttcttt ctaaatgaag tatttataat tctacgactg tcatacttcc tgtatcaaca
     4921 ttgtaaatag caccagagat aatgacatcg tctggtatta ggggagactc gataagcagt
     4981 tgcatatcct cgcgtacact ctcttctata tcttggaagg gcaagaagtc ctggtctgac
     5041 acatctacac ctaattcctc ttttaaatac tcctgaaaag gttcattttc aaaggtctga
     5101 gcaccacagt ctgtatggtg caataccaca atctctcttg tccccatttg ttgctgggaa
     5161 ataactagcg aacgaatcat gtcttcagtc actcgaccac ctgcattccg caagatatga
     5221 gcatccccaa gtgccaaacc tagagcttgc gcaacgtgca aacgtgagtc catacaggtc
     5281 acaatagcta ctcttgtttt gggtttaagt ggcagattta actgcccatg tagggcaaca
     5341 taagcctgat tagcttgcat aaactgttca aaatacgaca cgattccctc cttgaaaatt
     5401 tgatagtcaa atatttctcc tatcttatca tttttaagag aatttgtcac ggattatgca
     5461 aagacctttt tcaaaacttc ctgaatcgtt gtcacgccaa tgacctgaat ttccttaggc
     5521 agagtgattc ctgtcaagga attcttaggt acataaatct tagtaaagcc cagtttagca
     5581 gcttcgttga tgcgttgctc aatacgattc acgcgccgaa tctctcctgt caagcccagt
     5641 tctccgacaa aacattcctg aggattagtt ggcttgtctt tgtagctcga agcaatagca
     5701 actgcaacag ccaagtcaat ggcaggttca tccaatttaa caccaccagc agatttgaga
     5761 taggcatcct gattttgcaa gagaagccct gcccgttttt ccaaaacagc cataatcaag
     5821 ctagcacggt taaaatcaag tcctgtcgta gtacgcttgg catttccaaa catggtcggt
     5881 gttaccaaag cctgaacctc cgccaaaatc ggacgcgtcc cttccatggt tacaacgatg
     5941 gaggaaccag ttgccccatc caaacgctct tctaggaaaa cttgactcgg attgagtacc
     6001 tcaaccaagc cgcccgactg catctcaaaa atcccaatct cattagtgga accaaaacga
     6061 tttttgaccg ctctcaaaat acgaaaggtg tggtgacgct ccccttcaaa gtaaagcacc
     6121 gtatccacca tatgctccaa catacgaggc ccagccaagg ttccttcttt ggtcacatga
     6181 cctacgataa agatggcaat gttattggtc ttggccaact gcatgagttc agcggtcact
     6241 tcacgtacct gagaaacaga cccctgcacc cctgaaatct caggagacat gatggtctgg
     6301 atggaatcaa taataagaaa gtctggctgg atacgctcca cttctgcacg aacactctgc
     6361 atattggtct ctgcatagag ataaaactca ctatcaatat cacctaagcg ctctgcacgt
     6421 agtttaatct gctgggcaga ctcctcccca ctgatataga gaactgtccc cacttgggac
     6481 aactgggttg agacttgtag gagaagagtt gatttcccaa tcccaggatc cccaccgatg
     6541 aggacgagac ttcctggtac cactccgcct ccaagcacac ggttgaattc ctccatctcc
     6601 gtcttggttc gattgacatt gatggaagtc acctcagcta gtttcatggg cttggttttc
     6661 tcacctgtca aggacacacg cgcattcttg acctcggcaa cctcaacctc ttccacaaaa
     6721 gaagaccaag acccacagtt ggggcaacgt cccagatatt taggggaatt atacccacaa
     6781 ttttgacata caaatgtcgc ttttttcttt gcgatgacaa acctctttct atatctctaa
     6841 ctcacactca atcacttggc aaaaatcaat cttctcattt ggcacaaact ggcgcatgag
     6901 cattcgatga gcaacaacta ccacagtctg atgttctcga tacttagaca tacattctag
     6961 aaaccgagac ttcatttccg tagctgtctc atattgaata ggactattag gaagcaactc
     7021 ccccttgttt tctaaaaaca gtcttctagc tgtttcaaag ttttctattc ctgttttata
     7081 gacctgccat tcatgtaata aaggctctac tcttaaagga agacccgtag cacagaccac
     7141 atacgaagcc gtttctaaag ctcttgtgac tgcagaagat acgattattt cagctgacga
     7201 gagtaaagga gttttgctca atttctggac ttgctgccgt cccatctcag acaagggtgc
     7261 caaatctatc ccaaatccta tataagaacg ctcctctaac tcacggtaat ctggctcccc
     7321 atgacgtaca aagataatct tcattctagt gccctgtcga tccaaatcca ccagttcgaa
     7381 cgccatcagc tgcatctcca tctgcaatta agaaagtagc aaaaacagcc tggacaatac
     7441 gttccccaac ttcaagaaca acctcttggt ctgtgatatt cttcatctgc gcaaaaatat
     7501 gcccttcatt tccaggattt ccataataat ccccatcaat gaccccaact gagttaatta
     7561 aaaccaagcc cttcttacga ggatttgaag aacgatcata gaggtagaga acctcagtcg
     7621 gctgcatata agccttaacc cctgtcggaa ccaagacaat ctctcctggc gcaacaactg
     7681 tacgcacagc aacctttaag tcgtaaccag ccgcatgcgc tgtctcacgc ttgggcaata
     7741 aattttcatc tgtaaaactc gaaaccaatt caaaaccacg aattttcata attttctctt
     7801 ttctattatc atttattcta gattattcta tcttatttat tcggaaaaag cacgaaaaaa
     7861 gagcacacaa ttcaaatcgc ttagggctgc tggattcctc ccctgacccg cttcacgcag
     7921 aactgttgct ccactattta ttatatcaca ttcctattca ttttaaaagc aaaattattt
     7981 tttccgtcta tttctaaaaa aatcctgcat gatagctgcg cattcatctt ccaaaattcc
     8041 tatttcaacc tccacacgat gattgagacg ctcatctgtc aagatattgt acaaactccc
     8101 agcagcgcca aatttctggt ttttagcccc atagaccaca tttggaatgc gggcaagccc
     8161 aatcgctcca ctacacatga cacaaggttc aatggtcaca aaaagtgtgc aatccagcaa
     8221 gcgccagctc tcctcactca agttcgcatc ctctatagcc ataatttccg catgcataac
     8281 cgctcgctgt aattcctcac gcgcattatg cccacgacca atgatttccc catctttgac
     8341 aatcacacaa ccaattggaa tttcatcgtg ttcaagagca atctcagcct ctctcaaagc
     8401 ctccctcata aagacttctt tttcttcaag cgtataatac atctcttttc tcttttccta
     8461 cttatcgatt ttattattat atcatgaatc ccaacacaaa agaagccacc gaatgcggtg
     8521 actctatagg gagattatta tgaaaaagaa aagtttagga tatttgttac aacaagttag
     8581 gaggtcttct tgtaaccgtc tatagtatac ccgacctatc ttaaacaaat cttaaaaatc
     8641 tcttaggacc aaacactttc taaaatattt gtttgttcac gaccaggacc tactgagaaa
     8701 gtagaaatac gaacgccaac caattcactc acacgacgaa catagttacg cgcattctca
     8761 ggaagatctt ccaaattgcg aactccggta atatcttctg accaacctgg caactcttca
     8821 tagataggct tgcaacgttt caattgctca agactagctg gatagtagtc aatacgttga
     8881 ccgtcaagat cataggccac acagattttc acagtatcca aaccgctcaa aacatcaata
     8941 gagttcaaag aaaggttagt aataccagaa acacgacggc tatgacgcat cacaactgag
     9001 tcaaaccaac ctacacgacg tggacgacca gttgttgtac catattcatg acccacttca
     9061 cggatacgtt ctcccacttc atcaaacaac tcagttggga aaggaccatc tcctacacga
     9121 ctcgtataag ctttacatac acctacaacc ttgtcaatct tgcttggacc gacaccagaa
     9181 ccaattgtca caccaccagc tacagggttt gatgacgtaa caaatggata agtaccttgg
     9241 tcgatatcta gcataacacc ttgtgcacct tcaaaaagca cacgtttgcc attatcaagc
     9301 gcatcattca agataacaga tgtatctatc acgtatttct tgatttgttg accatattcg
     9361 taatattctt caaaaatatc atcgaaaaca atcgctttac tgtcatacaa tttttcaaaa
     9421 agacgattct tttcagcaag gttacgttct aaacgctcac ggaaaatatc tttatctaaa
     9481 agatctgcaa tacgaattcc aacacgagca gccttgtcca tataagctgg accaattccc
     9541 ttaattgtcg taccaatctt attgtcgccc ttagcttctt cttgcaagcg atccaactcg
     9601 atatgataag gcaaaataac atgcgcacga tcagaaatac gcaagttatc agttgttaca
     9661 ccttcctcat gaagatagct caactctttt acaagagatt taggatttac aaccatacca
     9721 ttcccaatga cagatatttt ttcagggaag aaaatcccag atggaatcaa gtgcaactta
     9781 aatttcttac cgtcaatcac aatcgtgtga ccagcattat caccaccttg gtaacgtgca
     9841 attacttctg cattcgctga aaggaagtct gtaatcttcc ctttaccttc atcaccccat
     9901 tgggtaccta caacaacaac tgaagtcata attttgtctg agccctcagg ctcttccttt
     9961 ctcacataca tggcaggact ctcacctgca attatatctt acaatttatt ataataaaaa
    10021 atcgcctttt tatcaagaag aaacaataga aagatttgct atttccaact attaaaaaat
    10081 gatttagaaa aattactagc tatttactat tatctttcca taaaagagta aattagttcg
    10141 gaaatttact aaaattacct caacaagaaa taaacccccg attcattacc aatttttcaa
    10201 gatacaaacg ataagcaaca cgataatggt aaacgataaa atccctacga caaccaatgc
    10261 catatctcac taaataataa attaaaaatt taaaatgaac atgttcccag tcaaaattat
    10321 caccaaatgt aggaccatac tcttcttcaa tactatcata gaaattagcc atctgctcat
    10381 aaattttttg taacataatc aaatactcct tttctttttt ataaacttat tctaacaaaa
    10441 aattttacac attcactatc aattcctgaa ttgttaaaat accagcctca acaagataaa
    10501 aataggaaaa gttgacaaaa tagaaacaaa tttgctacct aaatttcaaa caattaaagt
    10561 tctaaaaaga ccgccttaaa acttttgggt aaatcctatt aaaactataa tatttttata
    10621 taggtattat ggttctgatt ttctagtata aaaaaattca aactagaatt ctccttagaa
    10681 agcgtcgatt caagtgctat cactgttcaa aaatgatggt cgctgaaact tctatcgtca
    10741 agaagaatca ccaaatccct cgtattatca accaaaagat tgctcaaaag ttaattgaaa
    10801 agatttctat gaccgacatt gatcatcagt tgtctatttc aacttcaact gtcattcgca
    10861 agatcaatga ttttcacttt gagcatgatt tttcgcgtct tcctgagatt atgtcctagg
    10921 acgttgaaac agtctgggga gtgactgttt caatcgggag atagagatga gctttattgc
    10981 tcaggacttt gataagctca atatcataac tgttcttgag agcagaacac aagccatcat
    11041 ccgaaatccc atgaatacaa ggctatcaag cgctactgga gctcattcaa caagatagtc
    11101 agaaactgag tgataaacga ttttatcgcc ctacttttcg tatgcacttg actaataaag
    11161 aaattctaga taaacttttg agctattcga agacttgaaa caccactata atctctatca
    11221 gctataatct ctatcagctc ttgcttttcc acctcttttt cagactctgt ttaaaacctt
    11281 cctcaaagat aaagagaaaa tcgtcaacgc ccttcaatta ccttattcca acgccaaact
    11341 ggaagccacc aataatctca tcaaacttat caagcacaat gcctttggtt ttaggaactt
    11401 tgaaaacttc aaaaaagaaa ggacgaaatt tgtcctttct agatcttcgc tttcttcaac
    11461 ccactacagt tgacaaagaa ccgaaaaatg tctcgcctct aatatagtca attgaaacaa
    11521 gaacaagaga aaagagcctc gtaaaaggta ttgcaacttg gtaatacctt tttgaggttc
    11581 tttttgatat aagcccatgt tttctcaata ggattgtact caggtgagta gggaggaaga
    11641 ggtaaaagtt tatgcccaaa ctcctcgcat aaaagttcta acttacccat tctatggaat
    11701 cttgcattat ccataataat aaccgatggt gtggttaatg ttggtaagag aaacttctga
    11761 aaccaagctt caaaaaagtc gctcgtcacc atctcttcgt aagtcattgg agcgattaac
    11821 tcaccatttg ttagacctgc aaccaaagaa atcctctgat atcttcttcc agatacttta
    11881 cctcttatta actgaccttt taatgagcga ccatattctc gataaaaata agtatcgaat
    11941 cctgtttcat caatctaaac aggtgctagg tgctttaaac tattaaaatt tttaagaaat
    12001 aaggctactt tttctgggtc ttgttcatag taggtgtggt tctttttttc gagtgtagcc
    12061 catagctttg agcgcatagt ggatggtagt tggatgacag ccaaattcag aagctatttc
    12121 a
//
LOCUS       DAVKQB010000056        11526 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKQB010000056 DAVKQB010000000
VERSION     DAVKQB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.056"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..348
                     /locus_tag="VW528_001764"
     CDS             1..348
                     /locus_tag="VW528_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU9912546.1"
                     /translation="MKQIALESHSIVPQFSRGLPKNLLEISQIVNNLEGKVTNRQLIS
                     LLPFVEDPDAELEALEEEKKKNMEDMPMFNQENTKPEDEVEDEESGVLGEEESQSDLP
                     ADGQGRKAGRPVR"
     gene            257..1894
                     /locus_tag="VW528_001765"
     CDS             257..1894
                     /locus_tag="VW528_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEU9912547.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEV
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPNDPNIQRLLADLDSPA
                     YSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDLMKDSYYKATFDLQQQTGLAYG
                     FSGLPDSEIKHLQFFSWVGDGSTYSTNIWKNTGKLTSSIKDELLMSLMTGRDTRETAQ
                     AIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEADIEKYIFVAVLDKRTSRICQEH
                     DNQVYDRDKAVPGVNCPPMHPWCRSTTVGYDEDADYSKLKRRARNPETGKVEYVPADM
                     TYKEWYSKYVAKDGEKVYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKL
                     AGLEFRKKTVESLSRKIIADSLVENISLSKAVSKINDALRYTTIFDSDTFTEEYLKMK
                     QKLIAEGYKIVKVKNTWLVDGPYKGVNTVVEKDGINFEMQYHTQESFDLKNGSLHELY
                     EKYRDTNTSDLERMKLFKEMLDLSNGLEIPKNIERVK"
     gene            1896..2105
                     /locus_tag="VW528_001766"
     CDS             1896..2105
                     /locus_tag="VW528_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912548.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSMDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            2102..2515
                     /locus_tag="VW528_001767"
     CDS             2102..2515
                     /locus_tag="VW528_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9912549.1"
                     /translation="MIDIALAIAKKAHAGQVDKAGVDYIQHPLYVASQVNTEQEKAVA
                     LLHDVIEDSDITAADLFASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIYYLST"
     gene            2558..2845
                     /locus_tag="VW528_001768"
     CDS             2558..2845
                     /locus_tag="VW528_001768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912550.1"
                     /translation="MNKYKKLIELIENNGLEIQSKKCYDPQSAWHGEELWIVDKKKQN
                     KIFDLSGNGYCFHDAKVEEAIEEVEKYLLLKKMDTFDDFKKWVEKNAKPKK"
     gene            2986..3198
                     /locus_tag="VW528_001769"
     CDS             2986..3198
                     /locus_tag="VW528_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912551.1"
                     /translation="MEEWKERFKKEYYELKERFQKLDMMIGKYEKGQLEFESKCPIDL
                     LKGQRSTMWNYLRILEQRAKIEEIKL"
     gene            3368..3892
                     /locus_tag="VW528_001770"
     CDS             3368..3892
                     /locus_tag="VW528_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEU9912552.1"
                     /translation="MSEEINATVSTESTETVDTQENVDTVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTNAETVSESITSIQKAWEESVQKGVSERMKGSAPIKNAQT
                     VQQEVTEKWRKDFL"
     gene            3915..4940
                     /locus_tag="VW528_001771"
     CDS             3915..4940
                     /locus_tag="VW528_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEU9912553.1"
                     /translation="MAFEELNTAESRKKHLGIIEDVLAVNSYSTPLVTSSDAVTLQGR
                     SFTVATGNTTELKDYKRNKDNEFDHVEVEEKVYTLDEEKYWGRFVDQLDERDSNGQVN
                     INYVIARQAAEVVAPYLDELRFGAALGNVSDNVAMGKTAGANNAYNAVLDVSEKLDEL
                     GITKERLLFVTPSFYKAIKSEIVRLPHGDADKKVLGKGYVGELDDYTVYKVPSKFLKG
                     VNALATAPGVVTSPVQVDNTKYNDNIPGRFGELVEQLLYTGAFVLEHFKKYIITIADS
                     KPAAKKSVQGKVVNRAKAWKTGTTYKEGESVTHADKVYVAVKDISNSATAPDTDTTNW
                     KEKIGKK"
     gene            4950..5138
                     /locus_tag="VW528_001772"
     CDS             4950..5138
                     /locus_tag="VW528_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912554.1"
                     /translation="MKFKIKQDFYDWESNVKRLVGEELEITEERYAELADNFASNGVA
                     ISDVLEEALPEPEFLEED"
     gene            5140..5451
                     /locus_tag="VW528_001773"
     CDS             5140..5451
                     /locus_tag="VW528_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEU9912555.1"
                     /translation="MSIELLKKMTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGVEGEHSRTEGGIAVVYGENGLSTGLLQRIRMHRLARVAGHVFEA
                     E"
     gene            5432..5770
                     /locus_tag="VW528_001774"
     CDS             5432..5770
                     /locus_tag="VW528_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912556.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYVKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHKVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            5745..6260
                     /locus_tag="VW528_001775"
     CDS             5745..6260
                     /locus_tag="VW528_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU9912557.1"
                     /translation="MSELLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELIISIKTRAKMDGDRAIGEVYTNLKYAPYVEFGTGPIGQANHSGISPEV
                     SVTYKSNPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            6257..6625
                     /locus_tag="VW528_001776"
     CDS             6257..6625
                     /locus_tag="VW528_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912558.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINKIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGVVDLDSELVFQFRMEN"
     gene            6628..7041
                     /locus_tag="VW528_001777"
     CDS             6628..7041
                     /locus_tag="VW528_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9912559.1"
                     /translation="MLANGIKLAFSETKGNYQNLVGLKEVPEFGIEPEKVENTTLADT
                     VKKYEFGIGDAGELEYKFAYNNSSATAPYRVLRKAADGKKKLYFEQTYPDGTKVTFEG
                     QVSVKLGGGGVNAVIEFTLKIALQSNLTFTDGVGG"
     gene            7046..7426
                     /locus_tag="VW528_001778"
     CDS             7046..7426
                     /locus_tag="VW528_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912560.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSFEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKNKKTSKKKMTVVE"
     gene            7465..7815
                     /locus_tag="VW528_001779"
     CDS             7465..7815
                     /locus_tag="VW528_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912561.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKTQERKEKIIDSYR
                     LSQMISNHVSLLLSNDAKIVEFWEYAPELFVEEQQAVELERQKQALLLHKERMREFAE
                     RHNRKRKEEVNGNS"
     gene            7802..11455
                     /locus_tag="VW528_001780"
     CDS             7802..11455
                     /locus_tag="VW528_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912562.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSNSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSAAMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGNTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANG
                     LGRQKERITRALVALFDNVGNLSEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVVPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLAILGLLADTIKLVADGFTVFSDWCKENKEPIVALITTWQTINFLSWAEQAGG
                     LAGAFSLLGSKISSIVGGIKNLGLAIKALTFDKLVSFAETIYLNTLYAKDFVVNSGKT
                     IAQLGKTALELGKSALAWTAHAAKMGLATAAEFAHSVAAGVATAATWAFNAALAVLTS
                     PITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICQAIGEFFSGLWTK
                     LQEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQ
                     RWADVTSALANIGAWFTDMFQKAWTGLTNIFSKLGSWFGERWNDVTSALSKVASWFGD
                     IFGKAFDAVKNAFSSIGDFFKGVWDTVKSIFVNAGQMVGEAVGGAFKSAVNAVLGTIE
                     NVVNGFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAMIGEAGKEA
                     VVPLENTGFIQTLGRVVSSAVVNAMAGVSPQGGFSGDGDIVIQIAGHEFGRVAIQEIN
                     KEHERAGQTLLKI"
BASE COUNT         3704 a         1981 c         2763 g         3078 t
ORIGIN      
        1 atgaagcaga ttgctttaga gtctcattca atcgttccac aattcagtcg tggtttgcct
       61 aagaacttgt tagaaatctc tcagattgtg aacaacttgg aaggcaaagt gaccaataga
      121 cagcttattt ctctcttgcc gtttgtggaa gaccctgacg ctgagctgga agccttggaa
      181 gaagaaaaaa agaagaacat ggaagacatg ccgatgttca accaagaaaa cacgaaaccc
      241 gaagacgagg tagaggatga agaatcagga gtattgggcg aagaggaaag ccaatctgat
      301 ttaccagcag atggacaagg ccgaaaagca ggcagaccag ttcgataagg tctatcagga
      361 agccaagact tacttggata aggaagtcaa taagattttt gataagttcc aacgtgatta
      421 tggtctaagt caggtagaag ctagacaagt cttgaagaac atgaaagaca agaaaaatct
      481 gaatgaactt cgtaaagtac ttgaagcgag accgaatgat ccaaatatcc aaagactact
      541 ggctgactta gatagtccgg cttattcttt ccgtatgaag cgtctagaac gtttgagtga
      601 tgatttagac cgtatgcgtg aatctatcta tcattcagaa aagacaggct cagacgcctt
      661 ttatagcgac ttgatgaagg atagttacta caaggctacc tttgacctgc aacagcagac
      721 aggactagca tatggctttt ctgggcttcc tgatagcgag attaaacatc tacagttttt
      781 cagttgggta ggagatggaa gtacgtactc aacaaacatc tggaagaata cagggaagct
      841 tacatcaagc ataaaagatg aactactcat gagtctcatg actggtcgag atacacgaga
      901 aactgcacaa gcaattgctg agcggttcaa tgtaggccag aatgatgcaa ggcgtttggt
      961 tcggacggaa tcagcctttt ttcataacca gatggaacta ctcagctatg aagaagcaga
     1021 catagaaaag tatatctttg tggccgtctt agacaagcgt acatcacgga tttgtcagga
     1081 gcatgacaat caggtctatg atagggacaa ggctgtccct ggcgtcaatt gtccacctat
     1141 gcatccgtgg tgcaggtcta ctactgtcgg atacgatgag gacgcagact acagcaagtt
     1201 gaagcgcaga gcaaggaatc cagagacagg taaagttgag tacgtgcctg ccgatatgac
     1261 ttataaagag tggtatagca agtatgttgc gaaagacggg gaaaaggtgt ataatcaaga
     1321 tacaagagaa gccaaggcga aattttatag cgaacaacta ttgtccaaaa tttcaggagt
     1381 tgagccaaaa attacaagtg atatgcaacg tatcgcagga gaaaacaaat tggcaggtct
     1441 tgaatttagg aagaaaacag ttgagtcatt atcacgtaaa attattgcag atagcctagt
     1501 tgaaaatata agtttgtcaa aagccgtgag taagattaat gacgccttaa ggtacacaac
     1561 tattttcgat tccgatactt ttacagaaga gtatttgaag atgaaacaga agcttatcgc
     1621 agaaggttat aaaattgtaa aagtaaaaaa cacttggcta gtagatggac catacaaagg
     1681 tgtgaataca gtcgttgaaa aagatggtat caactttgaa atgcagtatc atactcagga
     1741 aagtttcgac ttaaaaaatg gttcattaca tgaactctat gagaagtatc gtgatacgaa
     1801 tacatctgat ctagaacgca tgaaattatt taaggaaatg cttgatttaa gcaatgggct
     1861 tgagattcct aaaaatatag agagggtgaa gtgatatgaa agatattaaa tactaccgca
     1921 caacgacgaa caatgctcaa gtacttcgtt tgattgatgg tgtcatgcaa gtttttgaca
     1981 ttgaaaaaaa gtgggttaat agcatggatt ggtttaataa aatctttttt aatgacttta
     2041 cggattttga agaaatttca gaaaatgatg catttactta tattgacagg atggtagcgg
     2101 catgattgat attgccttgg ctatcgctaa aaaagcacat gcagggcagg tagataaagc
     2161 gggtgttgat tacatacagc atcctctcta tgtggccagt caagtcaaca ctgaacaaga
     2221 aaaagctgtc gctcttttac atgatgtgat tgaggatagc gatataactg ctgccgattt
     2281 attcgcgtct ggcttgtcaa atgaagttgt tacagcggta caaattttga caaagaaaaa
     2341 aggtcaaagt tatcaagaat atcttgggaa agtaaaatca aataatttag caagagttgt
     2401 aaaacttgca gatttgaaac ataactcaga tttatcacgt ttgaaatctg ttaccaatac
     2461 agactacgag cgtgttaaaa aatataaaaa tgcaatttat tacttaagca cctagagaaa
     2521 tctaagtgct tttttcgtgc tcagaaagga gaatctgatg aataagtaca aaaagttgat
     2581 agaattgatt gaaaataacg gtcttgagat acaatctaag aaatgttatg atccacagag
     2641 tgcttggcat ggtgaggagt tatggattgt tgataagaaa aaacaaaata aaatttttga
     2701 tttatcaggt aacggttact gttttcatga cgctaaagtt gaggaagcca ttgaagaagt
     2761 tgagaagtat ctattattga aaaagatgga tacgtttgat gatttcaaaa aatgggtgga
     2821 aaagaatgct aagcctaaaa aatgatgctt agaaaggagt aaagacatgt ttatatggga
     2881 ttgggtatca atcgcctttg ggtggttggt atttttgttg ttaatattta ttattatggc
     2941 cgtaatcagc ggaataatta aaggtgtaaa gaaaggaaca gaaaaatgga agaatggaaa
     3001 gaaagattta aaaaagaata ctacgaattg aaagaacgat tccagaagtt agatatgatg
     3061 attgggaaat acgaaaaagg gcaactagag tttgaatcta aatgtccgat tgatttgtta
     3121 aaaggtcagc gttcaaccat gtggaattat ttaagaattc tagaacaacg tgcaaaaatt
     3181 gaagaaatta aactataaaa attaaccgca tcgaaatcga ggcggttttc ttatgctcta
     3241 accgtatgga atcccgtacg gtttttatat tgtccaaact gtgccgatga cattaaaagc
     3301 tgtactgttc cgtcgccgga cgtaaagcga gattatcgag tggcgacgta atcgctggag
     3361 gacaattatg tcagaagaaa tcaatgcaac tgtatctact gaatcaactg agactgtcga
     3421 cactcaagaa aatgttgata cagtgcagga agaaaagcac gaacgaactt tcactcgtgc
     3481 tgaaatcggt aagatgctat ctgccgagcg ctctaaatgg gaagctgagc aagaagccaa
     3541 ggaaaacgaa gctaagaaac tcgccaagat gaacgctgat gagaaacaga aatatcagtt
     3601 agatcagcgt gagcaagaac tagccgaccg tgaaaaggcc attgctcgta aggaattgac
     3661 cgcagaggct aaagcaatgc taagtgaacg tgacttacct gttgagttag taaatgtagt
     3721 cgatttgaca aacgcagaga cggtatctga atctatcacc tctatccaaa aagcatggga
     3781 agagtcagtt cagaagggag tctctgaacg tatgaaaggt agtgcaccta tcaaaaatgc
     3841 acaaacagtc cagcaagaag tcacggaaaa atggcgtaaa gacttcttgt aataaaagaa
     3901 aagaggaaaa ataaatggca tttgaagaat taaacacagc agaatcacgc aagaaacatc
     3961 ttgggattat tgaggatgta cttgcagtaa attcatattc aacaccactt gtgacatcaa
     4021 gcgatgcagt aaccttgcaa ggtcgctctt ttacagtagc aactggtaac acaacagagt
     4081 tgaaagacta caaacgtaac aaagacaacg aatttgatca cgttgaagtt gaagaaaagg
     4141 tttataccct tgatgaagaa aaatactggg gtcgtttcgt agatcaattg gacgaacgtg
     4201 actctaatgg tcaagtgaat atcaattatg ttattgcccg tcaggcggca gaagtagtag
     4261 ctccgtatct tgatgaacta cgttttggtg cagcacttgg aaacgtaagt gacaatgttg
     4321 ccatgggtaa aacagcagga gcgaacaacg cttataatgc ggttcttgat gtgtctgaga
     4381 aacttgatga gcttggaatt acaaaagaac gcttgctctt cgtcactcca agtttctaca
     4441 aagcgatcaa gtctgaaatc gttcgtctac cacatggtga cgcagataag aaagtccttg
     4501 gaaaaggata tgttggtgaa ttggatgatt acacagtcta taaggttcct tctaaattcc
     4561 tgaaaggtgt taatgccctt gctactgctc caggtgttgt tacatctcca gtacaagtag
     4621 ataatactaa gtacaacgat aacattccag ggcgatttgg cgaattggta gagcaattgc
     4681 tttacactgg tgcatttgtt cttgaacact tcaagaaata catcatcaca attgcagatt
     4741 ctaagcctgc tgctaaaaaa tcagtacaag gtaaagttgt aaaccgtgct aaagcgtgga
     4801 aaactggaac aacttacaaa gaaggcgaat cagtaactca tgccgataaa gtctatgtag
     4861 cggttaagga tatttctaat tcagcaaccg caccagacac agatacaact aactggaaag
     4921 aaaaaattgg taagaaatag gtccgagtta tgaaatttaa aatcaaacaa gatttctatg
     4981 attgggaatc aaatgtgaaa cgactggtag gggaggagct tgagattact gaggaacgat
     5041 atgccgagct ggctgacaat tttgccagta atggtgtcgc tatctcagac gttcttgagg
     5101 aagccctccc tgaacctgag tttttagaag aggattgata tgtctataga gttgctgaag
     5161 aaaatgacag gcgaagaaga tactcagctt ctcatgttgc tccaaacgag ggctacaaat
     5221 cttatcttgt cagagactaa tcgcacatct ttgacacctg ctttaagtct cttaatacct
     5281 gaggttgcta tcgagctcca caaccgctca ggagtggaag gagagcattc tagaaccgaa
     5341 ggtggtatag cagtagtcta cggagaaaac ggcctgtcta cgggcctttt acaacgtatc
     5401 cgcatgcaca gactagcaag ggtggcaggc catgtttttg aagcagagta gactgaaacc
     5461 ttatccaatg cgacggtttg aaaagactgt cacagaggaa ggtgtcgcaa aagaaggata
     5521 tgtcaaggaa gctgagacag tccgtcttga attgtggcca gctagtagca agttgcaatc
     5581 tgaattgtat ggtgagcgtg tcaatgatat tttgaatgcc aatgccaaca aatcagctac
     5641 tatcaaagtg aaagatggtg tgtgtatcga tagccagaca gaagtgactc ataaggtcat
     5701 ttctaaaaag gtctacacac atcatcaagt tttggagtta gagcgtgtca gagctactag
     5761 gggcagatag gcttatagct aaatgtagac gattggctag taaaaaaact ggcgaggata
     5821 tcgtcttacg tgcggtacac aatgctacta taaaggttgt ccaagcagat gcaagaagac
     5881 tcgcaccagc gagagatgga gagcttataa ttagtatcaa aactagagca aaaatggacg
     5941 gagatagggc tataggtgaa gtttacacta atctaaaata tgctccttac gttgaattcg
     6001 gaacaggacc aataggacaa gctaaccatt cgggtatctc tccagaggtc agcgtgactt
     6061 acaagtctaa tccttggtat gtgcatgaag accaaatcaa tgtaggaccg taccactttc
     6121 aaaagattgg ggagttctac aagatgtatg gtcaacctgc tcagccttat ctttatccag
     6181 ctttgagaga caatcaagag cgtgtgtcta agaatatttc gaattatgtc cgtagaaaga
     6241 taagagaaca aataaaatga ttaatatcaa gcctgttatt tataaagaat tgcaaaaggt
     6301 tgcagataat gtgactgata cttatcctag cgattgggag actttcccag tcgttatttt
     6361 tttagaagaa caaaacaagc cgggtgattg gtttgatgac caggaacaaa aatcctctat
     6421 ccgctacaag gtggatatct ttgatgatac cagcactagt gagttagctg ttaaaatcaa
     6481 taagattttt gagtctttag gtttacgcag aaccgactgc caagacgtgc cagacccgtc
     6541 tcatttgaga cataaggtca tgcgctttga aggtgtcgtt gatttagact cagagcttgt
     6601 ttttcaattt agaatggaga attaaacatg ttagcaaatg gaattaaatt ggcctttagt
     6661 gaaacgaaag gcaattatca gaatcttgta gggcttaagg aagtacctga atttggtatc
     6721 gagcccgaaa aagtagaaaa tactaccctt gcagatacag tgaagaagta cgagtttggt
     6781 atcggggacg caggagaact tgagtacaag ttcgcttata ataattcaag cgcaacagct
     6841 ccttaccgtg tattacgtaa ggcagcagat ggtaagaaaa aactttactt tgagcaaaca
     6901 tatccagatg gtactaaggt cacttttgaa ggtcaagtat ctgttaagct tggcggtggc
     6961 ggtgtcaatg ccgttatcga gttcacactt aagattgcct tgcagtctaa tcttacattt
     7021 actgatggtg ttggaggtta attaaatggc gttaaaatac acaacttgga aagttactga
     7081 cgaaaaagag ttgaagctac gtttgacatc tcatcaagct gcaactgtgg aagaaaaaat
     7141 cggcatgaac ttgttaaaga ttttcatgcc tgaagctggc gaagagttca ctttaccgcc
     7201 tttgaaagtt atgctgttgt tagttcatgg agccttgcag cagtatgaac atgggtattc
     7261 ctttgaagat gtctacgacc tatacgatga atacgtggac aatgggggag accaaacaac
     7321 tttcatgaca gaggttttaa tgccactatt tgaagtatcg ggttttactc cacgaggaag
     7381 caagaacaag aaaacttcca agaagaaaat gacagtagtc gagtaatctt aacagtaacg
     7441 cagattattg agaggcttta tcctatgttt ttggacattg gaggcaagcc tcttgatttt
     7501 tgggatttga cggtgcttga aatcagagaa atgattgaaa gctacaaccg tgtcaaaacc
     7561 caagagcgta aagagaagat tattgactca tacagacttt cgcagatgat ttccaatcac
     7621 gtttctttat tactgtccaa tgacgctaag attgttgagt tctgggagta tgcgcctgag
     7681 ttatttgtag aagaacaaca agcggtagaa ctggaacgac agaaacaagc acttttgttg
     7741 cataaggaac ggatgcgtga atttgcagag agacataatc gaaaaaggaa ggaggaagta
     7801 aatggcaact cttgatgaat tgaaagtcat gattgacgct gagatagcgc ctttcaggaa
     7861 gaagatgaaa gaagtcgaga atcaggtcaa aggaacatct gaccaagtga agaatgccac
     7921 tgccaaagtt cgtgaacagt cgaactctat cggtagtgcg tttggtaagc tagctaagtt
     7981 cgctggtttt gcaatccttg gtaagaaatt acttgatgtt gggatgtatt caacgcagac
     8041 tgctcttgaa gtgtcagcgg ctatgaacca aatcaagcgc cagatgggcg agagttcgca
     8101 atctttctta aaatgggtta acgataacgc caacgctatg aacatgggtg tgggtgaggc
     8161 tactaactac ggtgcggtct actcaaactt attttctggg tttatcaaag ataccaacaa
     8221 gctaagcgcc tataccgcta agatgttgca gacatcggca gtggttgctg aaggttcagg
     8281 gcgcacgatt acagacgtta tggagcggat tcgctcaggt ttgctaggga acaccgaagc
     8341 aattgaggac ctaggaatca acgtcaatgt ggctatgatt gagtctactg aagcctttaa
     8401 gaagttcgca aacggacaga gctggcaaca gttggattac caaacccagc aacaaatccg
     8461 ccttatggct attctggaac aggctacagc caagtatggg aataccttgt ctaattctgt
     8521 aaatggtcgt atcagcctat ttaagtcgct gatgaaggac gcagcattga accttggtaa
     8581 ctctatgtta ccgattatca atgccattat gcctgtcttg aactcttttg ctatggtttt
     8641 gaagaacgtg acggctaaac tcgctgagtt tatcgctttg atgttcaaca agaaagcaac
     8701 agtgaaagat ggtgttggtg gagcagttgg agacatgggt aacgccatga aagacgctgc
     8761 aggcggagca ggagaccttg ctgacgcagt agacgacgct ggagattcag caggaggact
     8821 tgctgacaat cttggagact ccgccaaaaa cgctaagaag gccgctaaag agttgctagg
     8881 tcttttggga tttgatgaga ttaacatctt gcaaaaacca aaagatgacg acgcaggcgg
     8941 ttctggaggc ggtggcaaag gtggtaaagg aaagggaggc ggtggcggac ctttcaaaga
     9001 catcttgcca gaagtcgagt tgaccgacat ggacaacaaa ttcaagagca tttttgatgg
     9061 tcttggagat aagctcaaag ggttgtttga cctcttcaag aaaggttttg atgcagcatt
     9121 tagaccagaa ggtataaaac gcattaagac tgccttagac caaatagcta agacaatggg
     9181 agaaatcgcc actgacccaa gggttgtgaa tgcctttaac cgaatggctg agaaaattgc
     9241 ttatgcttta gggcaagtga caggctcaat aaccactatc gggctaggta tcggtgtttt
     9301 ccttgccgaa agtattgcaa atggccttgg aaggcaaaaa gaacgcatta ccagggcgct
     9361 agtcgctttg tttgataatg ttggtaacct ttccgaggca gtaggaaaca tagctcagga
     9421 cttttctagt gctttctacg acgtcattac ctcaactggt gcggttcgta tcggtagcgc
     9481 tattgtgtca actctgttga gtttgacatc taccattgtt gaagttggta gtaaattagc
     9541 aggaagtttg tttaaaggtt ttgaaaaagt cgttgtgaca agcgctccta aaatttcatc
     9601 agtcttccaa agtttattag atactgttgc gcctgtattt gagagcattg aaaggtctgt
     9661 taacaaattt ggcgatggct taagtcgtgt ttatgatgaa catgtagtcc ctgctattaa
     9721 ctctattgct aatgctttta atgggctaat tgacattatt cagattctct gggagaattc
     9781 ctggcaacct tttgctgagt ttttatcagg agtattcggt gttagtattg aaggaatttc
     9841 agatttatta ggaggtggcc ttttagccat tttgggacta ttggcggata ctattaagtt
     9901 agtggcagat ggtttcaccg ttttttctga ctggtgtaaa gaaaacaaag aacctatcgt
     9961 agctttgata acaacttggc aaacgattaa tttcttatca tgggcagaac aagctggagg
    10021 acttgcagga gcattcagct tgttaggtag taagatctct tcgattgttg gagggattaa
    10081 gaatctaggt cttgctatta aagcattgac atttgataag ttggtcagtt ttgctgaaac
    10141 aatctatttg aacaccttat atgcaaaaga ttttgtggtc aattcaggta aaacaattgc
    10201 acagctagga aaaactgctt tagaacttgg taaatcagct ctagcatgga ctgctcatgc
    10261 agcgaaaatg ggattagcaa ccgcggcgga atttgcacat tctgttgcag caggagtcgc
    10321 tacagctgca acatgggctt ttaatgcagc gttagcagtt ttgacaagtc caataacatg
    10381 gattattgca gcaatcgcag ctttgattgc tattggtgtt ttgctctatc aaaactggga
    10441 cactgttgtt gagtttgcta aaactgcatg gcaaggacta tgtgatttta tcagtggtat
    10501 ttgtcaagcg attggcgaat ttttcagcgg tctatggacg aaactacaag aaatctttga
    10561 gccgataggt caatggtttg gcgagaaatt ccagcaagca tgggacgcca ttgtaaacat
    10621 attctctggc atcggagagt ggttctctgg tgtattccaa ggtgcatggg acgctatcgt
    10681 taatatcttc acaccaatcg gctcatggtt cggacaacgt tgggcagatg tgactagtgc
    10741 gttggctaat atcggggcat ggtttactga catgttccaa aaagcatgga ctggcttaac
    10801 aaacatcttt agcaaactag gttcttggtt tggcgagaga tggaacgatg ttacaagtgc
    10861 actttccaaa gtagcaagct ggtttggcga tatattcgga aaagcttttg acgctgttaa
    10921 aaatgccttt agctctatcg gagacttctt taaaggcgtt tgggatactg tcaaaagtat
    10981 cttcgttaat gctggtcaga tggtcggaga ggcagtaggt ggagcgttta agagtgcggt
    11041 caatgcggtt cttggaacga ttgaaaatgt agtcaatggc ttcatcggaa tgattaatgg
    11101 agttttaggc gttgtcagaa acttacctgg tctaggatgg gttggtagtg taagcacagt
    11161 tagcctccct cgtcttgccc gtggtggtat cgtcgatagt ccaacaatcg ccatgattgg
    11221 tgaagctggt aaagaggcgg tcgtaccact tgaaaataca ggatttatcc aaacacttgg
    11281 acgagtagtc agcagtgcgg tagtaaatgc catggctggt gttagtccac aaggtggatt
    11341 ctctggcgac ggcgacatcg ttatccaaat cgcaggccat gagttcggac gggtagccat
    11401 ccaagaaatc aacaaggaac atgaacgagc aggtcaaacc ttgctcaaga tttaggaggt
    11461 taaatggcac aattgacaat caatggggtg gctgtgaagc ctcccaaatc ttttcaagtc
    11521 ggtatt
//
LOCUS       DAVKQB010000057        11290 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKQB010000057 DAVKQB010000000
VERSION     DAVKQB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11290
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.057"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            713..1705
                     /locus_tag="VW528_001781"
     CDS             713..1705
                     /locus_tag="VW528_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9912563.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1707..2525
                     /locus_tag="VW528_001782"
     CDS             1707..2525
                     /locus_tag="VW528_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9912564.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2527..3312
                     /locus_tag="VW528_001783"
     CDS             2527..3312
                     /locus_tag="VW528_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9912565.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3351..3629
                     /locus_tag="VW528_001784"
     CDS             3351..3629
                     /locus_tag="VW528_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9912566.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3614..3811
                     /locus_tag="VW528_001785"
     CDS             3614..3811
                     /locus_tag="VW528_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9912567.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
     gene            complement(4237..4329)
                     /locus_tag="VW528_001786"
                     /pseudo
     CDS             complement(4237..4329)
                     /locus_tag="VW528_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4543..10962
                     /locus_tag="VW528_001787"
     CDS             4543..10962
                     /locus_tag="VW528_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9912568.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     AENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDTKTSNEEAKIKEENSNK
                     SQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGSKAIKELSSLKNTKVLYTYDRIF
                     NGGAIETTQDNLNKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFG
                     KNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAF
                     NYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSY
                     KMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAG
                     IPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFD
                     KVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKI
                     AVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQV
                     FSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKE
                     IDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKST
                     YGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTAR
                     PMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLK
                     EIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVP
                     EIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEME
                     ALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIP
                     GTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSGSKIA
                     NIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHF
                     IRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQ
                     FEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTY
                     HKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQ
                     GRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKGMISY
                     YLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKEKSSF
                     TIDRNISTIRDFENKDLKKLIKKKYKEEDDFVTGGKRTVELDYKYDDKGNITAYEDES
                     ALEYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKFIEIKATK
                     YDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDDRIKAETKIRMPEKNKETKAEY
                     PYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKV
                     TTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLM
                     SALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEA
                     IKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVN
                     ESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYL
                     QTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFD
                     GEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSE
                     LKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYV
                     INLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDH
                     SQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLG
                     LKKKNQD"
BASE COUNT         3959 a         1683 c         2259 g         3389 t
ORIGIN      
        1 attatggtat cgaatcttca tcagaatgat aaaattaatc aattgatatc tgattacaaa
       61 cagaatatga aagcttttta tatcactatt gaaaaattta tacgagatga tgaaagcctt
      121 aagtgttatt ttataaaggt tatttcaagt cgttccaagg taacaagtct agatcagatt
      181 gaagctgata aaacgataca aagaaaatat tcaagtgagc taaaaaaatt tattggattt
      241 tataatgaga ttatttgtga ggaaaatagt ttcctacatg tacgaaagag gtggtcgagt
      301 tggtttaggt agtcgatgcg tgagttgata attctcaggg tatggacttc tttttcatga
      361 atgaggtaaa agagcaggta ttgtttagag acaatcattc tgagcatatt ttctggatag
      421 agggagtatc cgattttatg atcaaagtta ataccgccct ctggtgagaa gatgagtagg
      481 ttggtaattt aaactattaa acagaatttt tgattaaaag tattatttta tgagagaaat
      541 cctaatttca caatccatag gcaaacgctt gcatttcgtt ttttattgga ctataatagg
      601 ttggtataaa gccttttgta gtaataaaat gtagaaggtg tagaaagcaa ggatttagaa
      661 tatttgtagt taaaaacaca atgttgctat tccttacgat agggagatag atatggcaat
      721 gatagaagtg gaacatcttc agaaaaattt tgtgaagact gttaaggaac cgggcttgaa
      781 gggggctttg cgctccttta ttcatcctga aaagcagacc tttgaagcgg tcaaggattt
      841 gacctttgag gttccaaaag ggcagatttt aggatttatc ggggcaaatg gtgctgggaa
      901 gtcgacaacc attaaaatgc tgacaggaat tttgaaacca acatctggtt tttgtcggat
      961 taacggcaag attccccagg acaatcggca agattatgtc aaagatattg gcgtagtctt
     1021 tggacaacgc acccagctat ggtgggattt ggctctgcaa gagacctaca ctgtcttaaa
     1081 agagatttat gatgtgccaa actcgctctt tcataagcgc atggactttt tgaatgaagt
     1141 cttggatttg aaggacttta tcaaggatcc cgtgcggact ctttcactgg gacaacggat
     1201 gcgggcggat attgcggcct ccttgctcca caatcccaag gttctttttt tagatgagcc
     1261 gaccattggt ttggacgttt cggttaagga taatattcgt cgggcaatta ctcagatcaa
     1321 tcaagaggaa gaaactacca ttcttttgac cactcacgat ttgagtgata ttgagcaact
     1381 ttgtgatcgg attttcatga ttgacaaggg gcaagagatt tttgatggaa cggtgagcca
     1441 actcaaggag acctttggta agatgaagac tctctctttt gaactgctac caggtcaaag
     1501 tcatctcgtc tctcactatg aaggtctgtc tgatatgacc attgatagac aaggaaacag
     1561 cctcaacatt gaatttgata gttctcgcta ccagtcagct gacattatca agcaaaccct
     1621 gtctgatttt gaaatccgcg atttgaagat ggtggatacg gatattgagg atattatccg
     1681 tcgcttctac cgaaaggagc tctaggatga tcaaattatg gagacgttat aaacccttta
     1741 tcaatgcagg ggttcaggag ttgattactt accgagtcaa ctttattctc tatcggattg
     1801 gcgatgtcat gggggctttt gtggcctttt atctctggaa ggctgtcttt gattcttcgc
     1861 aagagtcttt gattcagggc ttcagtatgg cggatatcac cctctacatc atcatgagtt
     1921 ttgtgaccaa tcttctgact agatccgatt cgtcctttat gattggggag gaggtcaagg
     1981 atggctccat tatcatgcgt ttgttgcgac cagtgcattt tgcggcctcc tatcttttca
     2041 ccgagcttgg ttccaagtgg ttgattttta tcagcgttgg ccttccattt ttaagtgtca
     2101 ttgtcttgat gaaaatcata tcgggtcaag gtattgtaga ggtgctagga ttaactgtca
     2161 tttatctttt tagcttaacg ctcgcctatc tgattaactt tttctttaat atttgctttg
     2221 gattttcagc ctttgtgttt aaaaatcttt ggggttccaa cctacttaag acttccatag
     2281 tggcttttat gtcggggagt ttgattccct tggcattttt tccaaaggtt gtttcagata
     2341 ttctctcctt tttgcctttt tcatccttga tttatactcc agttatgatc attgttggaa
     2401 aatacgatgc cagtcagatt cttcaggcac tccttttgca gttcttctgg ctcttagtga
     2461 tggtgggatt gtctcagtta atttggaaac gggtccagtc ctttatcacc attcaaggag
     2521 gttagtatga aaaaatatca acgaatgcat ctgattttta tcagacaata catcaaacaa
     2581 atcatggaat ataaggtaga ttttgtggtt ggtgtcttgg gagtctttct gactcaaggc
     2641 ttgaatctct tgtttctcaa tgtcatcttt caacatattc cattcctaga aggctggacc
     2701 tttcaagaga tagctttcat ttatggattt tccttgattc ccaagggaat ggaccatctc
     2761 ttttttgaca atctctgggc actagggcaa cgcctagtcc gaaaagggga gtttgacaag
     2821 tatctgactc gtcccatcaa tcctctcttt cacatcctag ttgaaacctt tcagattgat
     2881 gccttgggtg aactcttagt cggtggtatt ttattgggaa caacagtgac cagcattgtt
     2941 tggactcttc caaaattcct gcttttccta gtttgtattc cttttgcgac cttgatttat
     3001 acttctctta aaatcgcaac agccagtatc gccttttgga ctaagcagtc aggcgccatg
     3061 atttacatct tctatatgtt caatgacttt gctaagtatc cgatttctat ttacaattct
     3121 cttcttcgtt ggttgattag ctttatcgtg cctttcgcct ttacagccta ctatccagct
     3181 agctatttct tacaggaaaa ggatgtgttc tttaacgtag gaggtttgat gttgatttct
     3241 ctggttttct ttgttatttc ccttaaactt tgggataagg gcttagattc ctacgaaagt
     3301 gcgggttcgt aaaagctaaa gtaagactaa aatcaagaaa gaaacttatg atgtttgtaa
     3361 ttgaagaagt caaggatgaa aatcaaaaaa aggcagttgt cgctgaggtt ttgaaggatt
     3421 tgccagaatg gtttggaatc ccagaaagca cacaagccta tatagaagga accacgacac
     3481 tgcaagtttg gaccgcctat caggagagtg atttgactag atttgtaagc ttatcctatt
     3541 cgagtgaaga ttgtgcagag attgattgtc tcggcgtaaa aaagcttatc aaggtagaaa
     3601 aattgggagc caattgcttg ctactttaga gagtgaagct cgtaaaaaag ttggttatct
     3661 gcaggtcaaa acagtggcag aaggttctaa taaagattat gatcgaacaa atgactttta
     3721 tcgaggtctt ggctttaaaa agttagagat ttttcctcaa ctatggaatc cgcaaaatcc
     3781 ttgtcagatt ttgattaaaa agcttgaata atattacttg acatctattc tcagagtgct
     3841 atactgtaag tgtaatcgcc gatttagctt agttggtaga gcaaggcact cgtaaagcct
     3901 aggttatagg tagataaacg actgaggatt tgaaaaaata gataggtaga agataaccgt
     3961 taagccttac tcttagcggt tatttatatt gtttaatagc gctaatattt tatcaattat
     4021 gcctgttttc gtgtttctgg tagttgttca agtttattgc tactattttt gatggtatga
     4081 atgtgcttat aatgtatccc ggttaacgaa agttttggac ttatactctt cgaaaatctc
     4141 ttcaaaccac gtcaacgtcg ccttgccgtg cgtatggtta ctgacttcgt cagttctatc
     4201 cacaacctca aaacagtgtt ttgagctgac tacgtcagtt ccatctacaa cctcaaaaca
     4261 ctgttttgag caatctgcgg ctagtttcct agtttgctct ttgattttca ttgagtacta
     4321 atcttatttt ttaatgtgtg ctaaaaaata aattaactaa tgttaatttt tttgacttaa
     4381 atttttttaa atgatatact gtttttatag agagataaca attatatata aatgtactac
     4441 tctatttcct agataatact tagtaaaact ttttataaca aaggctagca aagttaaagt
     4501 ttattatcta ttggaagtta tataaatatg taaggaatta aaatgaaaaa aagtacagta
     4561 ttgtcattaa ccacagctgc agttatttta gcagcctatg cccctaatga ggtagtctta
     4621 gcagacacat ctagctctga agatgcttta agcatctctg ataaagaaaa agtagcagaa
     4681 aataaagaga aacataaaga tattcataat gctatagaaa cttcaaagga tactgaagaa
     4741 aagaaaacaa caattattga ggaaaaagaa gttgttagta aaaatcctgt gatagacact
     4801 aaaactagca atgaagaagc aaaaatcaaa gaagaaaatt ccaataaatc ccaaggagat
     4861 catacggact catttgtgaa taaaaacaca gaaaatccca aaaaagaaga taaagttgtc
     4921 tatattgctg aatttaaaga taaggaatct ggatcaaaag caatcaagga actatccagt
     4981 cttaagaata caaaagtttt atatacttat gatagaattt ttaacggtgg tgctattgaa
     5041 acaactcaag ataatctgaa taaaattaaa caaatagaag gtattacatc ggttgaaagg
     5101 gcacaaaaag tccaacccat gatgaatcat gccagaaagg aaattggagt tgaggaagct
     5161 attgattacc taaagtctat caatgctccg tttgggaaaa attttgatgg tagaggtatg
     5221 gtcatttcaa atatcgatac tggaacagat tataggcata aggctatgag aatcgatgat
     5281 gatgccaaag cctcaatgag atttaaaaaa gaagacttaa aaggaactga taaaaattat
     5341 tggttgagtg ataaaatccc tcatgcgttc aattattata atggtggcaa aatcactgta
     5401 gaaaaatatg atgatggaag ggattatttt gacccacatg ggatgcatat tgcagggatt
     5461 cttgctggaa atgatactga acaagatatc aaaaacttta acggcataga tggaattgca
     5521 cctaatgcac aaattttctc ttacaaaatg tattctgacg caggatctgg gtttgcgggt
     5581 gatgaaacaa tgtttcatgc tattgaagat tctatcaaac acaacgttga tgttgtttcg
     5641 gtatcatctg gttttacagg aacaggtctt gtaggtgaga aatattggca agctattagg
     5701 gcattaagaa aagcaggcat tccaatggtt gtcgctacgg gtaactatgc gacttctgct
     5761 tcaagttctt catgggattt agtagcaaat aatcatctga aaatgaccga cactggaaat
     5821 gtaacacgaa ctgcagcaca tgaagatgcg atagcggtcg cttctgctaa aaatcaaaca
     5881 gttgagtttg ataaagttaa cataggtgga gaaagtttta aatacagaaa tataggggcc
     5941 tttttcgata agaataaaat cacaacaaat gaagatggaa caaaagctcc tagtaaatta
     6001 aaatttgtat atataggcaa ggggcaagac caagatttga taggtttgga tcttaggggc
     6061 aaaattgcag taatggatag aatttataca aaggatttaa aaaatgcttt taaaaaagct
     6121 atggataagg gtgcacgcgc cattatggtt gtaaatactg taaattacta caatagagat
     6181 aattggacag agcttccagc tatgggatat gaagcggatg aaggtactaa aagtcaagtg
     6241 ttttcaattt caggagatga tggtgtaaag ctatggaaca tgattaatcc taataaaaaa
     6301 actgaagtca aaagaaataa taaagaagat tttaaagata aattggagca atactatcca
     6361 attgatatgg aaagttttaa ttccaacaaa ccgaatgtag gtgacgaaaa agagattgac
     6421 tttaagtttg cacctgacac agacaaagaa ctttataaag aagatatcat cgttccagca
     6481 ggatctacat cttgggggcc aagaatagat ttacttttaa aacccgatgt ttcagcacct
     6541 ggtaaaaata ttaaatccac gcttaatgtt attaatggca aatcaactta tggctatatg
     6601 tcaggaacta gtatggcgac tccaatcgtg gcagcttcta ctgttttgat tagacctaaa
     6661 ttaaaggaaa tgcttgaaag acctgtattg aaaaatctta agggagatga caaaatagat
     6721 cttacaagtc ttacaaaaat tgccctacaa aatactgcgc gacctatgat ggatgcaact
     6781 tcttggaaag aaaaaagtca atactttgca tcacctagac aacagggagc aggcctaatt
     6841 aatgtggcca atgctttgag aaatgaagtt gtagcaactt tcaaaaacac tgattctaaa
     6901 ggtttggtaa actcatatgg ttccatttct cttaaagaaa taaaaggtga taaaaaatac
     6961 tttacaatca agcttcacaa tacatcaaac agacctttga cttttaaagt ttcagcatca
     7021 gcgataacta cagattctct aactgacaga ttaaaacttg atgaaacata taaagatgaa
     7081 aaatctccag atggtaagca aattgttcca gaaattcacc cagaaaaagt caaaggagca
     7141 aatatcacat ttgagcatga tactttcact ataggcgcaa attctagctt tgatttgaat
     7201 gcggttataa atgttggaga ggccaaaaac aaaaataaat ttgtagaatc atttattcat
     7261 tttgagtcag tggaagaaat ggaagctcta aactccaacg ggaagaaaat aaacttccaa
     7321 ccttctttgt cgatgcctct aatgggattt gctgggaatt ggaaccacga accaatcctt
     7381 gataaatggg cttgggaaga agggtcaaga tcaaaaacac tgggaggtta tgatgatgat
     7441 ggtaaaccga aaattccagg aaccttaaat aagggaattg gtggagaaca tggtatagat
     7501 aaatttaatc cagcaggagt tatacaaaat agaaaagata aaaatacaac atccctggat
     7561 caaaatccag aattatttgc tttcaataac caagggatca acgctccatc atcaagtggt
     7621 tctaagattg ctaacattta tcctttagat tcaaatggaa atcctcaaga tgctcaactt
     7681 gaaagaggat taacaccttc tccacttgta ttaagaagtg cagaagaagg attgatttca
     7741 atagtaaata caaataaaga gggagaaaat caaagagact taaaagtcat ttcgagagaa
     7801 cactttatta gaggaatttt aaattctaaa agcaatgatg caaagggaat caaatcatct
     7861 aaactaaaag tttggggtga cttgaagtgg gatggactca tctataatcc tagaggtaga
     7921 gaagaaaatg caccagaaag taaggataat caagatcctg ctactaagat aagaggtcaa
     7981 tttgaaccga ttgcagaagg tcaatatttc tataaattta aatatagatt aactaaagat
     8041 tacccatggc aggtttccta tattcctgta aaaattgata acaccgcccc taagattgtt
     8101 tcggttgatt tttcaaatcc tgaaaaaatt aagttgatta caaaggatac ttatcataag
     8161 gtaaaagatc agtataagaa tgaaacgcta tttgcgagag atcaaaaaga acatcctgaa
     8221 aaatttgacg agattgcgaa cgaagtttgg tatgctggcg ccgctcttgt taatgaagat
     8281 ggagaggttg aaaaaaatct tgaagtaact tacgcaggtg agggtcaagg aagaaataga
     8341 aaacttgaca aagacggaaa taccatttat gaaattaaag gtgcgggaga tttaagggga
     8401 aaaatcattg aagtcattgc attagatggt tctagcaatt tcacaaagat tcatagaatt
     8461 aaatttgcta atcaggctga tgaaaagggg atgatttcct attatctagt agatcctgat
     8521 caagattcat ctaaatatca aaagcttggc gagattgcag aatctaaatt taaaaattta
     8581 ggaaatggaa aagagggtag tctaaaaaaa gatacaactg gggtagaaca tcatcatcaa
     8641 gaaaatgaag agtctattaa agaaaaatct agttttacta ttgatagaaa tatttcaaca
     8701 attagagact ttgaaaataa agacctaaag aaactcatta aaaagaaata taaggaagaa
     8761 gacgactttg taactggtgg aaaaagaaca gtagaacttg attataaata tgacgataaa
     8821 ggaaatataa ctgcctatga agatgaaagt gctctagaat atgaaactga aaaatttgac
     8881 gaaataaaat caaaaattta tggtgttcta agcccatcta aagatggaca ctttgaaatt
     8941 cttggaaaga taagcaatgt ttctaaaaat gccaaggtat attatggaaa taactataaa
     9001 tttatagaaa tcaaagcgac taagtatgac tcacattcaa aaacgatgac atttgattta
     9061 tacgctaata ttaatgatat tgtggatgga ttagcttttg ctggagatat gcggtttttt
     9121 gttaaagatg atgatcgaat aaaagctgaa actaaaatta gaatgcctga aaaaaataag
     9181 gaaactaaag cagaatatcc ctatgtatca agttatggga atgtaatcga attaggagaa
     9241 ggagatcttt caaagaacaa accagacaat ttaactaaaa tggaatctgg taaaatctat
     9301 tctgattcag aaaaacaaca atatctgtta aaggataaca tcattctaag aaaaggctat
     9361 gcactaaaag tgactaccta taatcctgga aaaacggata tgttagaagg gaatggagtc
     9421 tatagcaagg aagatatagc aaaaatacaa aaggccaatc ctaatctaag agtcctttca
     9481 gaaacaacaa tttatgctga tagtagaaat gttgaagatg gaagaagtac ccaagctgta
     9541 ttaatgtcgg ctttggacgg ctttaacatt ataaggtatc aagtgtttac atttaaaatg
     9601 aacgataaag gggaagctat cgataaagac ggaaatcttg tgacagattc ttctaaactt
     9661 gtattatttg gtaaggatga taaagaatac actggagagg ataagtccaa tgtagaagct
     9721 ataaaagaag atggctccat gttatttatt gataccaaac cagtaaacct ttcaatggat
     9781 aagaactact ttaatccatc taaatctaat aaaatttatg tacgaaatcc agaattttat
     9841 ttaagaggta agatttctga taagggtggt tttaactggg aattgagagt taatgaatcg
     9901 gttgtagata attatttaat ctacggagat ttacacattg ataacactag agattttaat
     9961 attaagttga atgttaaaga cggtgacatc atggactggg gaatgaaaga ctataaagca
    10021 aacggatttc cagataaggt aacagatatg gatggaaatg tttatcttca aactggctat
    10081 agcgatttga atgctaaagc agttggagtc cactatcagt ttttatatga taatgttaaa
    10141 cccgaagtaa acattgatcc taagggaaat actagtatcg aatatgctga tggaaaatct
    10201 gtagtcttta acatcaatga taaaagaaat aatggattcg atggtgagat tcaagaacaa
    10261 catatttatg taaatggaaa agaatataca tcatttgatg atattaaaca aataacagac
    10321 aagacactaa acattaagat tgttgtaaaa gattttgcaa gaaatacaac cgtaaaagaa
    10381 ttcattttaa ataaagatac gggagaggta agtgaattaa aacctcatag ggtaactgtg
    10441 accattcaaa atggaaaaga aatgagttca acgatagtgt cggaagaaga ttttatttta
    10501 cctgtttata agggtgaatt agaaaaagga taccaatttg atggttggga aatttctggt
    10561 ttcgaaggta aaaaagacgc tggctatgtt attaatctat caaaagatac ctttataaaa
    10621 cctgtattca agaaaataga ggagaaaaag gaggaagaaa ataaacctac ttttgatgta
    10681 tcgaaaaaga aagataaccc acaagtaaac catagtcaat taaatgaaag tcacagaaaa
    10741 gaggatttac aaagagaaga tcattcacaa aaatctgatt caactaagga tgttacagct
    10801 acagttcttg ataaaaacaa tatcagtagt aaatcaacta ctaacaatcc taataagttg
    10861 ccaaaaactg gaacagcaag cggagcccag acactattag ctgccggaat aatgtttata
    10921 gtaggaattt ttcttggatt gaagaaaaaa aatcaagatt aagataaaag ctatagaaaa
    10981 aaatggttta tgtactgaga ttagatagtg aggtgatgac atagttttgt gaaaacagcc
    11041 atttataact caattattta gtttacttca ctattgatac tatttggagt tattaatgga
    11101 cttagtttag ataactaatg aattgattga aagggttagt attgacaata ttggtcatat
    11161 tgattagaaa atagagtcta tcaaaattta aaggctaata gaggtgatga gacaatttcg
    11221 gctctttgtc aactgtagtg ggttgaagtc agctaagctc gagaaaggac aaattttgtc
    11281 ctttcttttt
//
LOCUS       DAVKQB010000058        11119 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKQB010000058 DAVKQB010000000
VERSION     DAVKQB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11119
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.058"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            33..395
                     /locus_tag="VW528_001788"
     CDS             33..395
                     /locus_tag="VW528_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912569.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            930..1622
                     /locus_tag="VW528_001789"
     CDS             930..1622
                     /locus_tag="VW528_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9912570.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1675..4085
                     /locus_tag="VW528_001790"
                     /pseudo
     CDS             1675..4085
                     /locus_tag="VW528_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
     gene            4335..5036
                     /locus_tag="VW528_001791"
     CDS             4335..5036
                     /locus_tag="VW528_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912571.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDVLNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5038..7746
                     /locus_tag="VW528_001792"
     CDS             5038..7746
                     /locus_tag="VW528_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9912572.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQCSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATDQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMSQSAVSSVVQNASAIRLFDSVASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7842..8333)
                     /gene="tpx"
                     /locus_tag="VW528_001793"
     CDS             complement(7842..8333)
                     /gene="tpx"
                     /locus_tag="VW528_001793"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9912573.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8456..9385)
                     /gene="psaA"
                     /locus_tag="VW528_001794"
     CDS             complement(8456..9385)
                     /gene="psaA"
                     /locus_tag="VW528_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9912574.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9417..10265)
                     /locus_tag="VW528_001795"
     CDS             complement(9417..10265)
                     /locus_tag="VW528_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9912575.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10262..10984)
                     /locus_tag="VW528_001796"
     CDS             complement(10262..10984)
                     /locus_tag="VW528_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9912576.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3270 a         2072 c         2535 g         3242 t
ORIGIN      
        1 atttgaaaaa aaataaaaaa ggagatttta ttatgaaatc aagtggtatc gtcggtcttg
       61 tgggaggaat tttacttctt atttgtcctt ttgtcttgtt gggaacagcg gtaaacacag
      121 ctgctacaac tcttaatgga ggagctactg caggggcttt ttcaggtgtt gccttactct
      181 tgaatgcctt gaagattgca aatcttgttc ttggtatcat tgctattgtt tactataaag
      241 gagataagcg tgtaggtgca gctccgtctg tactaatgat tgtttctggt gaagttagtc
      301 tcattccatt cttaggatgg gttgggggga ttcttgctat tatcggagga tctctattcc
      361 ttgcaacatt gaagaaattc aaatcagaag aataaaaggt attttagcat gaaaagaaca
      421 aaaaagttta tcggtatagg agtagctcta ttatctcttt ctcttctagt tgcatgtgga
      481 acataaagtt caaagaatac ttcaacaagt aatgatgaga agacagtagc aacatccaat
      541 agttcaaaag aaacaatcac tttcgataca ccggttgtaa cagacgatgc gattgaatca
      601 atacgcactt atgcagatta tatagatctt tataaaaata tttttgatga ttattttact
      661 aaaactgagg aaggtttcaa aggcacagct atggaaaata atgactcgtt tactaaacta
      721 aaagagtcaa ctcaaaaatt attcgatgcg cagaaaaaaa ggttaaataa tgaagataga
      781 atagaaacaa ccaaaaacaa tgtgattgcc aaacattgtc aaacagtcct ttcctttttg
      841 gttttgacta gcttttttgt gaaaaattgt gtaaaataga atagataaac gaggggaaac
      901 ctcggaaaat ttaaaggaga atccatctaa tggtaaaatt ggtttttgct cgccacggtg
      961 agtctgaatg gaacaaagct aaccttttca ctggttgggc tgatgttgat ttgtctgaaa
     1021 aaggtacaca acaagcgatt gacgctggta aattgatcaa agaagctggt atcaaatttg
     1081 accaagctta cacttcagta ttgaaacgtg ctatcaaaac aactaacttg gctcttgaag
     1141 cttctgacca attgtgggtt ccagttgaaa aatcatggcg cttgaacgaa cgtcactacg
     1201 gtggtttgac tggtaaaaat aaagctgaag ctgctgaaca atttggtgat gagcaagttc
     1261 acatctggcg tcgttcatac gatgtattgc ctccaaacat ggaccgtgat gatgagcact
     1321 cagctcacac agaccgtcgt tacgcttcac ttgacgactc agttatccca gatgctgaaa
     1381 acttgaaagt gactttggaa cgtgctcttc cattctggga agataaaatc gctccagctc
     1441 ttaaagatgg taaaaacgta ttcgtaggag ctcacggtaa ctcaatccgt gcccttgtaa
     1501 aacacatcaa aggtttgtca gatgacgaga tcatggacgt ggaaatccct aacttcccac
     1561 cattggtatt cgaattcgac gaaaaattga acgtcgtttc tgaatactac cttggaaaat
     1621 aaaaaattgt aagtctagaa ttgatttcta ggctttttat gttagtatgg aagtatgata
     1681 aggaataaaa aacaagatta tgtactggcc tacaagcaac cagcttcaac cacttacatg
     1741 ggttgggaag aagaagcttt accgataggc aatggttctt taggagcaaa agtatttggc
     1801 cttatagggg ctgaacggat tcaatttaat gaaaaaagtc tctggtctgg aggtccactt
     1861 cctgatagtt cagattatca gggtggaaat cttcaggatc agtatgtttt ttagctgaga
     1921 ttcggcaggc tttggagaag agagattaca atctggctaa ggaactggct gagcagcacc
     1981 taattgggcc aaaaacgagt caatatggga cctatctgtc ttttggggat attcacattg
     2041 agttcagcca gcaaggtacg actttgtctc aggtgacgga ctatcagaga cagctgaata
     2101 ttagtaaggc acttgcgacg acttcttatg tctataaggg aacgcgattt gaacgtgaag
     2161 cttttgcgag ttttccagat gatctcttgg ttcaatgttt tactaaggaa gggttggaaa
     2221 ctctagattt tactatagaa ctatccttga cctgtgattt ggcttctaat ggaaagtatg
     2281 aacaggaaaa gtctgattac aaggagtgta agttggatat tactgattct catatcttga
     2341 tgaagggaag agttaaggat aatgatctgc ggtttgctag ttatctagct tgggaaacgg
     2401 atggagatat tagagtttgg tcatataggg ttcagatatc aggagccagt tatgccaatc
     2461 tcttcttggc cgctaagacg gattttgccc aaaatcctgc tagcaattat cgcaagaaac
     2521 tagatttaga gcaacaggtg agagacttgg tggacacagc taaagaaaag ggctataccc
     2581 aattgaaatc aaggcatatc gaggactacc aagccttatt ccagcgtgtt caattggatt
     2641 tggaagctga tgttgacgca tccactacag atgatttgtt aaaaaattat aagccacaag
     2701 aagggcaggc tttggaggag ctgttcttcc agtatggacg gtatttattg attagttcgt
     2761 ccagagactg cccagatgct ctaccagcta acctacaggg agtctggaat gcggtcgaca
     2821 atcctccttg gaattcggac tatcacttaa atgtcaatct gcagatgaat tattggccag
     2881 cctatgttac caatctccta gaggcggtct ttccggtcat caactatgta gatgatttgc
     2941 gtgtctatgg tcgtctagcg gctgtaaagt atgcaggaat cgtctctcaa aaaggtgagg
     3001 aaaatggttg gttggttcat actcaagcga ctccctttgg ttggacggca cctggttggg
     3061 attactattg gggttggtca ccagctgcca atgcgtggat gatgcaaacc gtttatgaag
     3121 cctattcatt ttatagggac caagactatc tcagagagaa aatttatccc atgttgaggg
     3181 aaacggttcg tttttggaat gcctttttac ataaggatca gcaggcgcag cgttgggtgt
     3241 cttctccgtc ttattcccca gaacatgggc cgatttcgat tggcaatact tatgaccaat
     3301 ctctaatttg gcagttattt catgatttta ttcaggctgc tcaggaattg ggactggatg
     3361 aggacttgtt gactgaggtt aaggagaagt ctgatttact aaatcctttg caaatcactc
     3421 aatctggtcg aatcagggag tggtatgagg aggaagagca gtattttcaa aatgagaaag
     3481 tggaggccca gcatcggcac gcttcccatc tagtgggact ctatcctggc aatctcttta
     3541 gctacaaggg acaagagtat attgaagcgg cgcgtgctag cctcaatgat cgtggagatg
     3601 gcggcacagg ctggtccaag gctaataaga tcaatctctg ggcgcgtttg ggagatggca
     3661 atcgagccca taaattattg gcagagcagt taaagacatc caccttgcca aatctttggt
     3721 gtagccatcc tccttttcag atagatggta attttggtgc tactagtggc atggcagaaa
     3781 tgttactcca gtctcatgca gcttatctgg tacctctagc tgccctacct gatgcttggt
     3841 caacaggttc tgtttcaggc ttaatggcac gtggacattt tgaagtgagc atgagctggg
     3901 aagataaaaa actcttacag ttgaccattt tatcaaggag tggaggagat ttgcgagttt
     3961 cttatccaga tattgagaag agtgtgatta aaatgaatca agaaaaaata aaagcgaaat
     4021 gcatggggaa agattgtatt tcggtggcaa cagcagaagg tgatcttgtt caattttatt
     4081 tttaagaaga tgttataagg cagtaatttg aaactgcctt ttaataagga tttaagaata
     4141 taagcagttt tcaactagtt gaaaaagcgt tataatgata ataggaagta atactcaatg
     4201 aaaatcaaag agcacaaaca aggaagctag ccgcaggttg ctcaaaacag tgttttgagg
     4261 ttgcagatgg aagctgacgt ggtttgaaga gagattttcg aggagtataa tttgtttgat
     4321 agagggtggg tctgatggct tatattgaga tgaaacactg ttacaagcgt tatcaggttg
     4381 gggacacgga gattgtggcc aatcgtgatg tgaattttga gattgaaaag ggggagctgg
     4441 ttattatcct tggtgcttca ggtgcaggca agtcaacagt gcttaacctt cttgggggaa
     4501 tggataccaa tgatgaaggg gaaatctgga ttgatggtgt taatattgcg gattatagtt
     4561 cccaccagcg caccaattac cgtagaaatg atgtggggtt tgtttttcag ttttataatc
     4621 tagtttctaa tctgacagct aaggaaaatg tggaactggc ttctgaaatt gtgacagatg
     4681 tcttgaatcc tgatcaggtc ttgacagatg taggtctggc tcatcgtcta aataactttc
     4741 cagcccagct ttctggaggg gagcaacagc gagtctccat tgcacgcgcg gtagccaaaa
     4801 atcctaaaat tcttctttgt gatgaaccta ctggagcctt ggattatcag acgggcaagc
     4861 aggttttgaa aattctccaa gatatgtctc gtcaaaaggg agcgacggtg atcatcgtga
     4921 ctcataatgg agctttggcg cccattgctg atcgcgtgat tcatatgcac gatgccagtg
     4981 tcaaggatgt ggtgctcaac cagcatcctc aggatattga cagtttggag tactagtatg
     5041 atcaagcgaa aaacttattg gaaggactta attcagtcct tcacaggctc caaggggcgt
     5101 tttttatcca tcttgatcct gatgatgttg ggatctctag ccttagtagg cctcaaagta
     5161 accagtccca acatggaggc gacagctaat gcttatttaa caactgctca aaccttggat
     5221 ttggcagtca tgtctaacta tggcttggat caagcagacc aagaagaact aaaacagacg
     5281 gagggcgcag aggtcgagtt tggctatttg acagatgtga ctatggataa tgggcaggat
     5341 gccattcggc tgtactccaa accagagcga atttcaacct ttcagctaag aaagggacga
     5401 cttcctcagt cagacaagga aatcgctttg gccactcatt tgcaaggcca atgcagcgtg
     5461 ggacaggaga ttagttttaa agaaaaagaa gagggtcatt cctctttaaa agaccatact
     5521 tataccatta ctggttttgt ggattcggct gaaatcctct cccagcgaga tatgggctac
     5581 gcaggaagtg gaagtgggac tctgacagcc tatggggtga ttttacctag tcagtttgat
     5641 cagaaagtct acaatatagc tcgtttgaaa tatcaagatt tagcaggttt aaatgccttt
     5701 tcatcagctt atgaagaaaa atccaagcaa catcaggaag accttgaaca aactttatca
     5761 gataatggca aggtacgtct gcaacttttg aaaaaagaag gacaagagtc tctagacaag
     5821 gggcaagaga cccttgacaa ggctcagact aatttgcagg aaggcaagcg tcgtttagca
     5881 gctgctcaag ctcgtataca ggctcaagaa agtcaactag ccttgtttcc tcaagttcag
     5941 agagagcagg ctagtgctca acttacccaa gccaagcagg aattgggcaa ggaagaagac
     6001 aaactaaagc aagctgaaca aaatctagcc caagaaaagg aaaaattaga aaaacatcag
     6061 caagtcttgg acgatttggc ggagccaagg tatcaggtct ataatcgtca gaccatgcca
     6121 ggtggtcagg gctatcttat gtatagcaat gcttcatcca gtattcgagc agtgggcaat
     6181 atctttcctg tggtacttta tgccgtagca gccatggtga cctttacgac catgactcgc
     6241 tttgtagacg aagagcgaac tcatgcaggg atttttaagg ccttgggtta ccgtagtaag
     6301 gatattatcg ccaagtttct cctttatgga ctagtagctg ggactgtcgg aacggctcta
     6361 ggtagtatac ttggtcatta tttgctagcc agtgtaattt caagtgtcat tacaaaaggc
     6421 atggtggtgg gagaaaccca gattcagttc tattggacct atagcttact agctcttgtc
     6481 ttgagctggt tggcgagtgt gttaccagcc tatctggtgg cttggaggga acttcatgcc
     6541 gaagcagccc agcttctact tcctaaacct cctgtcaaag gagctaaaat cttattggag
     6601 cgtatcggtt ttatctggcg tcgtctcagt tttactcata aggtaacagc ccgcaacatc
     6661 tttcgttata agcagagaat gttgatgaca atctttggtg tggcaggttc tgtagctctg
     6721 ctctttgcag gtttgggaat ccaatcttct gtagcaggag ttccgtctaa acagtttcaa
     6781 caaatccaac agtatcagat gcttgtctct gaaaatccta gtgcgaccga tcaggacaag
     6841 gtagagctag cagaagtgtt gaaagggcag gagatactag cctaccagaa aatctattct
     6901 aaaacgctag acaaggattt caaaggcaag gctggtcttc aaaacattac tcttatgatg
     6961 atagagaagg aagatttgac tccctttatc catcttcaac atcatcagca ggagctgaca
     7021 ttaaaagatg gcatcgttat tacagctaaa ctcgcccagc tggcaggtgt caaggttggg
     7081 cagactttag aaattgaagg taaggaacta aaggtcgctg ctattactga gaactacgtt
     7141 ggtcacttta tttatatgag tcaggctagc tatgagcaac tttacggaca gctaccccaa
     7201 gccaacactt atctggtctc attaagggat accagtgcaa ctagtatcga aagtcaggcg
     7261 ggcttgctta tgagtcaatc tgcggtgtcc agcgttgtcc aaaatgcttc agccattcga
     7321 ctcttcgact ctgtcgctag ctcactcaat cagaccatga ccatcttggt catcgtatcg
     7381 gttctattag ctattgtcat cctttacaat ctgaccaata tcaacgtagc tgagagaatc
     7441 cgtgaactct ccactaccaa ggttcttggt tttcataata atgaagtcac cctctacatt
     7501 taccgtgaga cgattgtgct atcccttgtg ggaatcgtac ttggtctggt agctggtttc
     7561 tatttacacc aatttttgat tcaaatgatt tcgcctgcga ctattctctt ttatccgcag
     7621 gtaggctggg aagtctatgt aatcccagtg gcagcagtaa gcatcatttt gaccttgctt
     7681 ggtttcttcg tcaattatta tctgagaaag gttgatatgt tagaagccct gaaatctgta
     7741 gagtaaggta gttattttta gctgattgaa cttctattta ctaatattca aaaatcctcc
     7801 gtttcaaaga gcagggaact ctttgtgaca gaggattttt tctatagggc tttagcagct
     7861 gcaattgcgg cttcgaagtt tggctcagaa ttgatattat ccacgtattc aacgtagcga
     7921 atcgtattgt cagtatcgag gacaaagact gcgcgtgcta ataggtgcca ttcgttgatc
     7981 aagagggcat aatcgcgccc gaaagaatgg tcaaagtagt ctgaaagcat aatggcattg
     8041 tcaaggcctt cagcaccgca ccaacgtttt tgagcaaaag gtaggtccat tgaaacagtc
     8101 aatacgaccg tgttgtccag tccagccaat tcttcattaa aacgacgtgt ttgagttgag
     8161 cagatgcctg tatcgataga aggaacgaca ctcaagactt ttttcttgcc atcaaaatca
     8221 gccagagatt ttttagaaag atctgttgta gtaagagaaa aatcaagcgc cttgtcgccg
     8281 acttgtagtt gtttacctgt aaagctcaca ggatttccga gaaaagttac cataggatac
     8341 tccaatcttt tttcttccat tgtatctgaa acagtcagaa ttttccaatg atttgaccgg
     8401 aaatgtgggc atagaaaaaa cgccagctca catgagaatg acgtttttca gaggcttatt
     8461 ttgccaatcc ttcagcaatc ttgtcaaggt tgtatttcat catgctgtag tagctgtcgc
     8521 cttctttacc ttgttctgcg atagagtcag taaagatttg tgcgtagatt gggatgtttg
     8581 tgtcttgaga aacagttttc attggacggt catccacact tgattctaca aagagtgatg
     8641 gaacttttgt ttggcgaagt ttttcaacca aggtcttgat ttgttcagga gttccttctt
     8701 cttcagtatt gatttcccag atgtaggcac ttgggacacc ataggcttta gagaagtatt
     8761 tgaatgctcc ttcgctggtt acaatgagtt tcttttcagc agggatctta ttaaatttat
     8821 ccttactttc tttatcaagt ttgtctaact tatcagtata ttctttgaga tttttttcat
     8881 agaattcttt attgttaggg tctttggcgc tcaattgttt ggcgatattt ttagcaaaaa
     8941 taataccgtt ttcaaggtta agccaagcgt gtgggtcttc ttttcctttt tcattttgac
     9001 cttcaaggta gataacatca acgccgtcgc tgactgcgaa gtagtctttg ttttcagttt
     9061 tcttggcatt ttctaccaat tttgtaaacc aagcattgcc acctgtttca aggttgatac
     9121 cgttatagaa aatcaaatca gcctcagaag ttttcttaac gtcttcagga agtggttcgt
     9181 attcgtgtgg gtcttgccca atcggaacga tactatgaag gtcaattttg tcaccagcaa
     9241 tatttttagt aatatcagcg atgattgagt ttgtagcaac aacttttagt ttttgaccag
     9301 aagttgtatc tttttttccg ctagcacatg ctacaagaat gattgcagaa agaaagagaa
     9361 cgagtaatgt acctaatttt ttcattagat cctccaattt attagggctt tgccccttat
     9421 tttaacaaat gtttattttt cagtttcaaa tatcgttgtt tgggagcgat aaagaagcta
     9481 atgagaaaga aactagcagc tgtaagcacg atactagaac ctgccgcaac attaaaacta
     9541 tagccaataa agagtcccaa aactgaagca gtagctccga aggttgagga aaggaaaatc
     9601 atgcttttca gactattagc atacagataa gcagttgcag ctggggtaat cagcatggct
     9661 acaatcagga tagttccgac actttgcatg gctgtcacag acacgagagt caggagtacc
     9721 atgagaaggt agtgatagaa attgacaggc attcccatgg ctttagccaa gagttcatca
     9781 aaggaagtta tcaagagttg cttgaagaaa atccagatta acaagaggat ggctgccccc
     9841 acacccatag taataaacat atccgtatct tggacggcca ggatattacc aaaaaggata
     9901 tggaaaaggt cagttgaact tttagcgaca ccaatcaaga tgataccgag ggctaagaaa
     9961 gaagaaaagg taatgccgat ggcggtatcg cttttgataa tcgagtttcc tttgatgtag
    10021 gtaatgatga tggcagctag caatccaaag acaatggctc cgataaagaa gtcaaggccc
    10081 aagatgaagg agagggctac acctggtaag acagcatgtg aaatggcatc tcccatgagt
    10141 gacatcccgc gtagaataat gaaacatccc acggctccag ctacaatccc gacgacaata
    10201 gctgttatca aggcattttg taggaaatgg aatttttgca atccatcgat aaattctgca
    10261 atcataggtc acctccattg aaaaagagtt gattaccgta agcttctttt agattggttt
    10321 cggtaaaagt ttcttttgtt ggaccaaagg caatcacttc tcgattgaca agtaagactt
    10381 gatcgaagta gtggggaatc ttgctgaggt cgtggtgaac gatgagaacc gtcttcccag
    10441 cttttttcaa atctctcagc gtattcatga tgatttcctc actgacagag tcaatcccag
    10501 caaagggttc atccaagagg atatagtcgg cttcctgcac caaacatctg gcaatcaaga
    10561 cccgctggaa ttgacctcca gacagttgac taatttgacg ttcagcgtag tcagctaggc
    10621 cgacgatttc aagggcctct tgcactttct tccaatgttt agcctttaaa cttcgaaaga
    10681 gaggaataga gggaaatagt cctaacgaga cgcattcctt gaccttgatg ggaaagttgt
    10741 agtcgatatt gattttttgt tcgacatagg caattcggtg taaggatttt ttaacttcct
    10801 tgtcatcgag aaatgcctga ccttgatgtg ggataattcc caacatacct tttaatagtg
    10861 ttgatttccc agcgccgttt ggaccaatga tgccggtaat tgttggtcca tggagcacta
    10921 gtgaaatatc cttaagtgcc aacgtttctt tgtaggagac actgaggttt tcgatacgta
    10981 tcataaactt gtattcctcc tgtctcttaa tatacattaa aaaaaaaatt aagtcaagtt
    11041 aatttttgaa aaaattaaaa taataactga aaaatagatt ctaaaaataa ctttcaggat
    11101 aaatttctaa attataaaa
//
LOCUS       DAVKQB010000059        11006 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKQB010000059 DAVKQB010000000
VERSION     DAVKQB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11006
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.059"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(766..1986)
                     /gene="divIB"
                     /locus_tag="VW528_001797"
     CDS             complement(766..1986)
                     /gene="divIB"
                     /locus_tag="VW528_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9912577.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQKDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGVYSYTVADKLIMEAEEKAKKEAEEAEKRQEEERKRLEEEQKKQEE
                     ESNRNQTTQRSSRR"
     gene            complement(1996..3054)
                     /locus_tag="VW528_001798"
     CDS             complement(1996..3054)
                     /locus_tag="VW528_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9912578.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(3058..4410)
                     /locus_tag="VW528_001799"
     CDS             complement(3058..4410)
                     /locus_tag="VW528_001799"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9912579.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQTASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(4514..5155)
                     /locus_tag="VW528_001800"
     CDS             complement(4514..5155)
                     /locus_tag="VW528_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912580.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(5157..7178)
                     /locus_tag="VW528_001801"
     CDS             complement(5157..7178)
                     /locus_tag="VW528_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9912581.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLIDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(7249..7548)
                     /locus_tag="VW528_001802"
                     /pseudo
     CDS             complement(7249..7548)
                     /locus_tag="VW528_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            complement(7912..8166)
                     /locus_tag="VW528_001803"
     CDS             complement(7912..8166)
                     /locus_tag="VW528_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9912582.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(8186..10027)
                     /gene="typA"
                     /locus_tag="VW528_001804"
     CDS             complement(8186..10027)
                     /gene="typA"
                     /locus_tag="VW528_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9912583.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPVEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            10189..10914
                     /locus_tag="VW528_001805"
     CDS             10189..10914
                     /locus_tag="VW528_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU9912584.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
BASE COUNT         3289 a         2406 c         1942 g         3369 t
ORIGIN      
        1 atcttaaata atatttcttc cctactaaca ctaataagtc cgatatggta aattcttgac
       61 ctattagttg ctctaaccat ttttttcctg tttcccaact aatacatgtt tcttactgga
      121 tggtatatat agatgatact gtccctcata aggagtaggt aatatcttaa tcttatcaac
      181 aattctcata acaaacctaa cctttaataa ataaaagagg ggctaagtaa aacatactct
      241 agtccctcag tccgcaatca ataaaataat gactaattct attaatctct tgctgcaaga
      301 ctaatacgga aaattcaaat gactgtctaa attgaaaaag cttgatatta gtaagtgaac
      361 aaacttcaac aaccagcaag agcaaggatt gctaaaccaa atccgatacc tgttgcaagt
      421 ccagctgaaa aatcactaac tggtactgaa atttcttcaa tttcctcaaa taaaaagtta
      481 tcttgcatga ttatcactcc ctatcttttt aacaacaggc tactccaatt aagagaacta
      541 ccccagttcc aagtaaaact cccccccaaa attcaccatc tgtaggggct acgatttctt
      601 caatttcttc aaataagaat acttcatctg acatatgtgt taccttcttc ataaaaaatt
      661 tttttgtaat cgattacatt gcaaatgtaa cataaagaaa aacagatgtc aaatattaaa
      721 cgtaaaaaca tggcaactaa agagctatag gagaaaaggt aaaacctagc gacgcgatga
      781 gcgctgggtc gtttggtttc gattgctctc ttcctcttgt tttttctgtt cttcttctaa
      841 acgtttacgt tcctcttcct gccttttctc agcttcttcg gcctcttttt tagctttttc
      901 ttctgcttcc ataatcaatt tatccgctac agtgtagctg tagactccag cttccatgtc
      961 aatcacactc ggttctgaca attgtggctt aatcttactg taatatggca gtttcttact
     1021 catttcagat agaggaacca agacttcgtc cgaatcattc atggtcaatc gaattaaatc
     1081 ggatgtcacc ttgcttgggg ctaattccac cttttggata gccgccttga gttctgggct
     1141 aatttgagca agttctgaga caaaaacctt gatttgttca ctatcattaa agagaactga
     1201 taaataagtt tctggtaaac tgttcagact cacagaacta gtctcaagct gaccactgga
     1261 aagaatagga taatgatttt caccagaaat atagtaggcc acaatatcat attccttgac
     1321 cttaatagtg aacttagttg gaaattgata gacaagttga gctgattcaa cccaatagtt
     1381 agacttaatc tgcttttcat attttgcctt gtctagcaga aggttaatcg tataatccga
     1441 atcctgaatg cctgaagcct gtcgaatatc atcagctgta gtttgcaccg ttccctcaac
     1501 acgaatatct ttcatggtcg cataaggact gagcaagtag gcagagacaa tcaataaaag
     1561 cagacttgga aataaaatcg tgaaggctcg caagatatgg ataccaggaa tctttgcttt
     1621 ggctggtttt tcctttgtag cctttttagc aagcttttta tcctgttcct ccttctcttt
     1681 agactctggt tcttctttct cttctttctc tttgtcagcc tctgaggatg ctactttttc
     1741 ttcagactct tccttagctg attctgaatc tttctggtct gtttcactct cctggtcctg
     1801 tttatcctct gacttctcag attcttctcc cattcgagct tgtctttcct tttccttctc
     1861 ctcagctaga gccgcctctt cttcagcctt cttttttaga tattcttggt ttcgtttctg
     1921 ccattctgat aactctttca attcttcgag ggtttctttg tcctcatttt tcttatcttt
     1981 tgacatttac tttccttatg ataaatcttt tttcaacaat tgataaaaat ctgctagaga
     2041 tttcaattcc ttagaagctt tcatcttagc ttggtaatct tccttgtgac ttagtaagtg
     2101 agaaagcttc tcttccaaac tatccaaggt caaatcgctt tcttgaaggt cttctgcata
     2161 gcctttctta acaaagtaag ctgcattttc aatctggtca ccacgactag cttcacgacc
     2221 aagcggcaca atgacatgca attttgctat cgccaagagc tcaaaaatcg tattggcacc
     2281 accacgtgtc acaacaatat cagccaattc catcaagggt tgatagagat cggtcacata
     2341 gtcaacacga aaaagatttt ggctcaactc attcaggcta gaatctccag ttagattgat
     2401 aatattgtag cgctctgtta gttctttttt atggtctgtc accaattggt taaagacacg
     2461 agcacctgca gaaccgccaa caaacaatac agtcggcaat ttatgattaa agtggctttg
     2521 aatatccacc aattcatctg gttctggatt tttttgatct gaaaccttgg tcactgctcc
     2581 cacatgctca accttagcca aactcgaagc ttgttcaaag gttgaataca tcttagtcgc
     2641 aaatttatag gcgattttat tggccaagcc catagacagg tcagattcgt gaataaagac
     2701 aggcactcct gacacacgcg cagcgataac aggcggtact gagacaaagc ccccctttga
     2761 aaaaagggtc tgtggacgaa gtcgcaacat gataaagagc gattggacga ttccccagcc
     2821 aactttgaag acgtccagca tattttgcca agagaaatag cgacgcaatt tcccagtcgc
     2881 aatggagtgg aaagtgacat ccaaacctga cttaaggatt tcttggtgtt cgataccacg
     2941 cttgtccccg atatagtgga cttcccaacc atcttcgatg aacttgggca ttaacaaaag
     3001 attgagggta acgtgtccaa ccgtcccccc acctgtaaag acaatttttt tcatatttta
     3061 ttcttttaac tccgctactg tgtcgataaa gaggtcgcca cgtacttcaa agttagcata
     3121 catatcccag ctggcattgg caggactaag aagaaccaca tctccttgag tcgcaagctc
     3181 ataggccttg cgggtcgcat ctgcaatatc tgtcgcctcc acataagcga caccagcctt
     3241 gtctgctgcc cgtttgacac gttctgcaga ttgacccagg atgaccatct tcttgagtcc
     3301 agtaatatct ggcaccaatt cgtcaaactc attgccacgg tccaaaccac ctgcaatcaa
     3361 gacgaccttg ctgttgtcaa atcctgacaa ggctttttga gtagccaaga tattagttga
     3421 tttactgtcg ttatagaatt taacaccctt gatgtcatcc acaaactgga gacggtgttt
     3481 gacaccaccg aaggctgaaa gagtttcctt gatggtttga ttgtccacac cacgaagctt
     3541 ggctacagca atagtcgcaa gggcattttc cacattgtgg ctacctggaa caccgatttc
     3601 attcgctgcc atgactactt caccacggaa gtagagttga ccatcttcca gataagctcc
     3661 atcaaccttt tcaagtgttg aaaatggtac aacagtggct tctgtcttgg aagtcaagtc
     3721 ttttgccaag tcttgattaa agttcaagac aaggaaatca gctgctgtca tcttgttctg
     3781 gatattccac ttggctgcta catattcctc aaataatcca tgataatcga tatgagttgg
     3841 cataaggtta gtaataaccg caatctctgg atgaaattct tgaacaccca tgagttggaa
     3901 agaagaaagt tccataacaa gcgtgtcctt gtccgacgca gtttgggcca cttgactagc
     3961 tggatagccg atattccctg ataaaagacc atgttgccca gcagcagtca aaacttcccc
     4021 aatcatagtc gttgtggttg tcttaccgtt cgatcctgtg ataccaataa tcggtgcttc
     4081 tgaaatcaaa taagccaatt ccacctcagt caagactgga attcccttgg ccaaagcctt
     4141 ttcaatcatg ggattgttgt aggggatacc tggatttttc accataaggg caaactcttc
     4201 atccaagagt tccaaaggat ggccacctgt aatgaccttg atcccttctt ccagcaaact
     4261 ttgggcagct ggattgtcct cgaatggttt cccgtcattt actgtcacta tggcgcctag
     4321 cttatccaac aaacgagctg cagattcacc agacttggcc aaacctaaaa caagaacttt
     4381 cttattttta aattgatcta ttactttcat gtctcgaact ccatttctac tcctactatt
     4441 ttaccatttt tatggaaata aaaaagccac aaagtgtgtt tgtgactctt tcttctaact
     4501 gaatcttacc atattatcta tgtgataaat cggtaactcg aatgacctga tccacttgct
     4561 cccaaatcag aggattgtgg gtcgcaataa taatggtccg attcggattt tttaaagatt
     4621 ctaggatgga aagtaattcc tcagagtttt tggggtctaa ggaagcggtt ggttcatctg
     4681 caagaatcaa aggcggatcc tttaaaatta tcttcgctaa tgcaacacgt tgtgcttctc
     4741 ctcctgataa ctcaaatata ggttgcttca aatccaaata agagaggttt acacggttta
     4801 gagcttgttt catcaaagag attttctctt tttccttcaa ctttttacca actaaaccca
     4861 gattgagatt ctctttgacg gtttggcttt caattaagcc aaaatcttga aataagtatc
     4921 ccaagtaatc tctaaagaaa acagaaggct tgatgtcctt aagagaagtg ccatcataga
     4981 tgatttgccc tttgtcatat ggctctaatc gtccaatcat attcaagagt gttgtcttac
     5041 cacagccact tgtaccgatt aaggcataaa ttttcccacc ttcaaaatga agatttgtat
     5101 ctgaaaatag ctgacggctt ccaaattttt tagatatatt ctttagttca atcatcctat
     5161 tttcctttca taattgtcat agaaacacga gattctttct gcgcttgacg gtaaagcgtc
     5221 agaactgcac tagctagaaa gactaataaa gtgagcaagc caatcaccaa gtctcgactg
     5281 cttaaaataa agagactagc accaaataca aaactggcaa attgactaac catatactga
     5341 gcatgtgttt caaaaaatcg taaacctgaa attcgtttaa tcaaaatatc tcggcggaat
     5401 tgctcgaaat atagaagatt gacagaataa aatagtaaca aggaactagc tatccccaca
     5461 atagctccta agattaaagt tgctgtttca gtttgaactt cattataacg agttagataa
     5521 acacttcttc cttctttaag ataggatact tgctcataaa ttccagcttt cttcaagagt
     5581 tctagcccac tctcatatcc tttgataaag agttgttttc cagcattgat agaccaacta
     5641 gataaggata taaaactatc acctgtagaa gtcggcgtga atacaactaa aatcggatca
     5701 atcaaatact gagtggatac gggattctca ccgttattat aaacaaaccg cttttctccc
     5761 attgaaagat aactaacgtg cgctttcatc tcataatcca aaggagcact tgcctcctca
     5821 ccagattttc cataataact caatctttct tcaaaaactt tcttaagttc tgcttctcga
     5881 gagcgcaaat gttctgggag caagaggata aactcacctt tttggagatg ggctaacttc
     5941 tgtttggtct cagcatctac cacgaccttt tccttgtcca aataactggg actaacatag
     6001 agcgtattag catctggact ataggtatcc agtgtctctc cctgttcatt ttttccttgt
     6061 ggattggcaa aatggagcag attatccttt acataaagag cttgttcttc ttcgattgct
     6121 tccttggcaa aggcatacca cttgctctga ttttctgtat cttttcctct atcacctaag
     6181 ccaaaagaaa tttgataata gtctgctcta tcctgccatg cttgttttga aatttcaagt
     6241 tctttcaatc gttggtaaga cgtcaaacct gtcttaacag cgtatcctac tgtaaagaca
     6301 gcaaccaact gacacaatag ggttaaaatc atcaggcgtt taaggggtaa tctcccctta
     6361 ataacgggaa ctaatgcttt gtaattcaaa ctcattagat aaagaagcat tagtaaaatt
     6421 gaaatcgcca ataaaaacaa cagatagaaa ctaatcccaa aaccataggt ggctaacaag
     6481 ataggataaa acaaaccttg actaaaaaga acgactcccc cacctaggaa ggaaaggagg
     6541 gctgatagaa ggagccattt gatatcagta gataaagaat gccccatgat ggataagaga
     6601 gtctgaccag aaaagagttt tatacctgct gctctcattt ccttaatccg agtgataatc
     6661 actaaagcaa agaaagataa accaaatatt gctaaactaa ttaaaataag gggatttagt
     6721 aatattcgaa aagcaagaaa atagggcggt atctttcggt cagcacttgc tttataaccc
     6781 aaatctccta atttatcggc aagcttttct ttcgtcaagg agcctgacaa aaggagataa
     6841 ctatttagcg gatcactacg ttcacgactt tcttggctag cttcttggaa ttcttttggt
     6901 aaagttccct gaccataagt tgcataagta aagtgagtcg tcccatcctt actcggctct
     6961 acaattcttc tagctattaa actctgttct gagtttgcaa aattctccaa ttcctgttca
     7021 aatacctcac gcgtcggttc ctgagtatct tttttgacac gaagtaaaga aacggaatca
     7081 tagcttgcat ataaatattg tggcgcacgt aagacaataa tccaagcaag gaagaagctg
     7141 agaaaaaaag ttgataataa tatgaatagt ttcttcatag tagactcctt gtaaacaaaa
     7201 ttccccctgt aatttcttac aaggggaacg atttaaatca atgaacgatt agtcataatc
     7261 acagtaaaat gctacttgtt ctccccattt agtccaaatc catgcatagg aggtttttcc
     7321 agcaggagct tctcctttat cagaatgacc atcccatctg ctcacgatgg atgaataatg
     7381 atatttttta ccatgatagt aatttgaaaa agcctaacca cctcctgaac cttctccata
     7441 tgtccatact cctccatctg gatattgtac agcagctgat gcagctccca ataatgtaaa
     7501 atttgaaata agagctagag caagtaatct atgttttttc gttttcattt tattttttct
     7561 ttcaaaaaaa tcacaccttg agcaacaatg caacaaaata aatcctcctc tctcttttgt
     7621 tgaaatcgct ttcttatgtg ataagaataa catttttatt atttgttgtc aaggaaaaaa
     7681 tcgaattttt tagatgtttt actatattat ctctatgaat aatattatat agtagtttta
     7741 tttcaaaata atatgcaacc agtactaacc aaatataaaa tagatgccat taacgaattt
     7801 tattcaagtt tttcccattc atactataca agtaaaagag atggtgttaa ctaaaaagca
     7861 attcaaacta ttgtaaaatt cctagcaaaa agagagccga aactctcttt tttatcttct
     7921 tttacttttt ttgactggca tgagtgtgat gtctctaaca ctaaagtaag ctaggatcaa
     7981 catggctatt gctaggaata tttctgttgg taattgaaaa attttcagaa aagatagaac
     8041 caataaaatc aagagtgcca ctaaaataca taccatagcg acgatattga cagtcccttt
     8101 aatgctttct ggtgtcgcaa atacatagag taggagcagt aaaattccta ggactaaata
     8161 gaccatcttt ctctctttct agctcttatt cagctgattt tttcttcttg ttagctttct
     8221 cacgctctgc tttgttaagg atttgtttac gcaaacggat agactcaggc gttacttcca
     8281 tgtactcatc gtcgttcaag aactcaagag actcttcaag tgtcaagata cgaggcgtct
     8341 tgataacagc tgtttggtcc ttagtagctg aacgaacgtt ggtcatttgt tttgccttcg
     8401 tgatgttaac tgtcaagtca ttttcacgag agttttcacc gatgatcatt ccttcataaa
     8461 cctcagtacc tgggttgaca aagatcgtac cacgttcttc gatagacatg attgagtaag
     8521 ttgtagcctt accagcatcg atagaaacaa gggcaccacg gtgacgtcca ccaatttccc
     8581 ctggaatcaa tggcaagtat tggtcgaagg tatggttcat gataccgtaa ccacgagtca
     8641 ttgacaagaa ctcagttgag tatccaatca aaccacgcgc tggaacaagg aagaccaaac
     8701 gagtttgacc attaccagtt gaaatcatat ccaacatttc acctttacgt tcagaaaggc
     8761 tttggataac agacccttgg tattcttctg gagtgtcgat ttgtacacgt tcaaatggtt
     8821 cacatttaac accgtcgatt tcttttacga taacttctgg acgagatact tgaagttcat
     8881 agccctcacg acgcattgtt tcgataagga ttgacaagtg caattctcca cgtcctgaaa
     8941 cagtccattt atctggtgaa tcagttgggt caacacgaag ggaaacgtct gtttgcaatt
     9001 ctgcctgcaa gcgttcttcc accttacgag aggttaccca tttaccttct ttaccagcaa
     9061 atggtgagtt gttgaccaag aaagtcattt gaagagttgg ctcatcgatg tgtaggattg
     9121 gaagagcttc tactgcatct gtcggagtga tggtttcacc gacaaagatg tcttccatac
     9181 ctgaaacggc aatcaagtca cccgctttgg cttcttggat ttcacgacgt tccaaaccaa
     9241 agaaaccgaa gagttttgta acacggaagt ttttagttgt accgtcaagt ttagaaaggg
     9301 taacttggtc cccaacctta actgtaccac ggaagacacg accgataccg atacgtccaa
     9361 cgaagtcatt gtagtccaaa agcgacactt ggaactgcaa aggctcatct gagttatcta
     9421 ctggagctgg gatatggtcg ataatcgtgt caaagattgg tgccatagtc gcttcttggt
     9481 cagctggatc atctgacaat gaagaagttc cgttgatcgc tgaagcataa accactggga
     9541 aatcaagctg gtcgtcatct gcaccaagct cgatgaaaag ttccaagact tcatccacta
     9601 cttctactgg acgagctgat ggcttatcga ttttgttaac aaccacgatt gggacaaggt
     9661 cttgttccaa ggcttttttc aatacgaaac gagtttgtgg catggttcct tcataggcat
     9721 ctacgaccaa gacaacaccg tcaaccattt tcatgatacg ctcaacttct ccaccaaagt
     9781 ccgcgtgtcc tggtgtgtcc ataatgttga tacgagttcc gttgtaagca acggcagtat
     9841 ttttagcaag gatggtaatc ccacgctctt tttcgatatc gtttgagtcc atagcacgct
     9901 ctgccaattc agtacgtgca tcaagcgttt ctgattgttt caataattcg tcaacgaggg
     9961 ttgttttacc gtggtcaacg tgggcgataa tcgcaatgtt acggatatct tctcttaatt
    10021 ttgtcatgat ttcctctata atattcaaaa tttattttct aactgaacga ttataccata
    10081 atttcaaata aataacataa ctcaagcaag tgtaaatgtt ttcactccgc ttttcttttc
    10141 acgtcaagcc ttttcaaagc aagcgactta tgataagata ggcacagtat gcgtttagat
    10201 aatttattag ctcaagaaaa aatcagccga aaggccatga aacaagcact cctcagaggg
    10261 gaaattctag tcgatggttg cccagcccgc tccctagctc aaaatatcga tacaggacta
    10321 caagaactcc tttttcagga tcgaatcatt caaggctatg aacacaccta tcttatgctt
    10381 cataaacctg ctggtgtcgt tacagccaac aaagacaagg aacttccgac cgtcatggac
    10441 ctgcttccat ctgacatcca gtctgacaag ctctatgccg tcggccgact ggaccgagat
    10501 acaacaggac tccttctctt gaccgataac ggtcccttgg gcttccaact cctccatccc
    10561 caatatcatg tcgataagac ttatcaagtt gaggttaatg gacttctaac acctgaccat
    10621 atccaagcct ttcaaaaggg aattgtcttt ttagatggta ctgtctgtaa acccgcaaaa
    10681 ctagagattc tatctgcaag tccctccatc agtcaagcct ctatcaccat ttcagaagga
    10741 aaatttcatc aaatcaagaa aatgttcctc tcggttggtg ttaaggtgac tagcctcaag
    10801 agaatccaat ttggggactt cacattgaac ccagatttag cagaaggtaa ctaccgccct
    10861 ttgaaccaaa aagagttaca aatcattaaa aactatttag agatgagtcg ataaaataaa
    10921 aaaagcttta aaactaaagc ttttttcttt tatttaccga aaaattaagg cgattgctac
    10981 aatccagtta actacagaaa tcacaa
//
LOCUS       DAVKQB010000060        10879 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKQB010000060 DAVKQB010000000
VERSION     DAVKQB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.060"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..711
                     /locus_tag="VW528_001806"
                     /pseudo
     CDS             1..711
                     /locus_tag="VW528_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(775..1857)
                     /locus_tag="VW528_001807"
     CDS             complement(775..1857)
                     /locus_tag="VW528_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9912585.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(1854..2831)
                     /locus_tag="VW528_001808"
     CDS             complement(1854..2831)
                     /locus_tag="VW528_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9912586.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            3046..3423
                     /locus_tag="VW528_001809"
     CDS             3046..3423
                     /locus_tag="VW528_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9912587.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            3658..5049
                     /locus_tag="VW528_001810"
     CDS             3658..5049
                     /locus_tag="VW528_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9912588.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            5163..6005
                     /locus_tag="VW528_001811"
     CDS             5163..6005
                     /locus_tag="VW528_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9912589.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            6278..7039
                     /locus_tag="VW528_001812"
     CDS             6278..7039
                     /locus_tag="VW528_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9912590.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            7232..8149
                     /gene="glyQ"
                     /locus_tag="VW528_001813"
     CDS             7232..8149
                     /gene="glyQ"
                     /locus_tag="VW528_001813"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9912591.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            8361..10397
                     /locus_tag="VW528_001814"
     CDS             8361..10397
                     /locus_tag="VW528_001814"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9912592.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            10439..10696
                     /locus_tag="VW528_001815"
     CDS             10439..10696
                     /locus_tag="VW528_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9912593.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
BASE COUNT         3346 a         1941 c         2411 g         3181 t
ORIGIN      
        1 tttcaggctt atatcgttcc tacacacgtg atgccttcta tcaactgttg aagcaccatg
       61 gttggcgaaa tattatgcca cgtccagaac atcctaggaa agcagacgct caaaccattg
      121 tcgcgtctaa aaataaaatc tcaattcaag aagacaagaa agcgctttaa aaccagtaga
      181 ccttttcata aggttcgctt gatgtaccaa gatgaggctg gtttcggtag aatcagtaaa
      241 ctgggatctt gttggtctcc aataggagta ggtccacatg tccatagtca ctatatacga
      301 gaatttcgct attgttatgg agctgttgat gccccataca ggcgaatcat ttttcttaat
      361 agctggtgga tgtaatactg agtggatgaa cgtcttttta gaagagcttt cacaagctta
      421 tccagatgat tattttttac tcgttatgga caatgctata tggcataaat caagtacctt
      481 aaagattccg actaatattg attttacctt tattcctcca tacacaccag agatgaaccc
      541 attgaacaag tgtggaaaga gattcgtaaa cgtggattta agaataaagc ctttcgaact
      601 ttggaagatg tcatgaatca actccaagat gtcatacaag gactggagaa ggaggtgata
      661 aagtccatcg ttaatcggag atggaataga atgctttttg aaagcagatg agtataaaat
      721 tgaattgctt ataaaaaagc tccatacact ggatgtgtat agagcaatgg ggctttattt
      781 gatatagagt tcttggtttt ttaggacaat ttctcggata cttgcaaact ttttaagttt
      841 tttgatttct tctggatgag tgacgagagt aataacatat ccttccttgc ccatacggcc
      901 agtacggcca gcacggtgtg tgtaggtttc gctatctcta ggaatatcaa agtttacgac
      961 acattctagg ctatcgatat caattccacg agccaaaagg tcagttgcaa gaagcagggt
     1021 tagttggtta tctttaaact tttctaagat gatttttcta aatttaacat taacatcact
     1081 agcgagggaa acagccaata tatcacgata ctgtagtttt tcctcggcat tcccaaggtc
     1141 tgacaggcta ttgaagaaga ctagaccacg gaaatcctct acatgagcca gttttcgcag
     1201 catatccact cgatgacgtt ggtctacctg catgtagaaa tgctggatat tgtccaattt
     1261 ttgatcagag agatcaatag tgcgtgtatt cggcacaatc ttttcttggt caaacttggt
     1321 cgtggcactc atgtagacca gttggtggtc acgaggtgcg tagtgagtga ttttttctac
     1381 aaagtgaatc tgagaatcat ctagtaattg gtcaaattca tccaggatga tggtttccac
     1441 attcatcatc ttgatttttt taagtttaat gagttcaaag atacggccag gagttccaat
     1501 cagaatttct ggcccctttt taagacgttc aatttgtcgt ttctgacttg aacctgaaag
     1561 gaagagttga gcagtcaatc cgatagcttc tgcccacgtt ttacatacat caaaaatctg
     1621 tccagcaagt tccgtatttg gtgctagaat caagagttgt tgggcttttt tcttttgtag
     1681 tctgagaaga cttggtagga gatacgctag ggtcttacca gttccggttt ggctcactcc
     1741 taggaggttt tctccagcaa gaaggggctc aaatagttga gtttgaatgg gggtgaattc
     1801 ttggaaaccg agttggtcac tcagttcttg ccattcagtc ggtagtttgg ttttcatttt
     1861 tctgcctcaa atctaatgcc agcagtctgg cgcatggtat atagtagctc atgaacagag
     1921 cctgcatcat acagccaagt ttggtagaga ttcagatctg gttgctggat catgtgtgca
     1981 aatgcagcga cttcctcagt catcgtatga ggagcctgtt ggataggaag ctggacttga
     2041 tttccttggt ggtcggtaaa aatagctgag cgaatatgct caatcgtgtt gagagtcaag
     2101 gttccatctg ttgtataaat ctcgcaagga agattggaag tgatgttttt tccagccttg
     2161 atgtgaactt gatagtctgg gtagaagagg ataccatctc catttaggtc aatgctattg
     2221 tcaagctgtt gagcatggta agtcgcgtca ttagcttttt caaaaagacg aacagcagca
     2281 tagaggggat aaatccccaa atccatgagg gctccaccag caaaacggtc tgaaaagaca
     2341 tttggtgttt gtccagccaa caagtcaggc atcttggaag agtatttggc atagttgaaa
     2401 tctgctccta acacttgctt atctgctaaa aagtttttga tagtagtaaa ggctttctcg
     2461 tggtaattac gagctgcttc aaagataaaa cagttatttt tttcagctgt ttgaatcaaa
     2521 tcaaaccatt cttgtggttg agtgacagct ggcttttcga gaataacatg tttaccagca
     2581 gacaaggcag cttttgcctg agcaaaatgt aaggagtttg gactggcgat atagactaaa
     2641 tcaaaagaag atttgaagaa gacttctaat tgatcgaata gttggatatt ctgatagcga
     2701 gaagcaaagg ttgctgcagt ttctagtttt ctagaataga ttgcgaccag ttggtattct
     2761 ccactggtat gggctgcttc tatgaaatga tggctgatag cgccagttcc gatgacacct
     2821 aattttagca taaatactcc ttttccgatt ttaaatcctt ctttcattat aacatagata
     2881 gacgggacta tccaacagag aggagaaaat ttcaaataag ctattagctt tcttttccga
     2941 ataaatagat agaagcatag aatctagcaa acctagattt aaaaatgtgc tataatagaa
     3001 ggaggaaaag gaggattctc agacatccag gtatcagccc aactaatgat ttgtcaattt
     3061 atccgcgata tgctggactt gccagcaaaa aatgtgacga ttttggaggg aagtaacatt
     3121 cacgtcttgc cttccatgcc ctactcagcg caagatttct atactagtat agacgtcttg
     3181 gcggagttag ataatggaat ccaagttatc atcgaaattc aggttcatca tcagaatttt
     3241 ttcatcaatc gcctatgggc ttatctgtgc agtcaggtta atcaaaacct agaaaaaatt
     3301 cgccaacgtg aaggtgatac ccaccagagc tacaaacaaa tcgcaccagt atacgctatc
     3361 gcaattgtcg atagtaatta cttctcagat gacctagctt ttcatagttt tatagtaaaa
     3421 tgaaatgaga acaggacaaa tcgatcagga caatcaaatc gatttctaac aatgttttag
     3481 aagtataggt ctactattct agcttcaatc tactagaaat tccatagata taaaactaca
     3541 taatctctac agatacggat gttggagttg atgtaagatg ctttggcttg ctagaggaat
     3601 tgtggattgc caaattgtat cattgaaatt attgctcaaa tttgttatga tataaatatg
     3661 aataaaagta gactaggacg tggcagacac gggaaaacga gacatgtatt attggctttg
     3721 attggtattt tagcaatttc tatttgccta ttaggcggat ttattgcttt taagatctac
     3781 cagcaaaaaa gttttgagca aaagattgaa tcgctcaaaa aagagaaaga tgatcaattg
     3841 agtgagggaa atcagaagga gcattttcgt caggggcaag ccgaagtgat tgcctattat
     3901 cctctccaag gggagaaagt gatttcctct gttagggagt tgataaatca agatgttaag
     3961 gacaagctag aaagtaagga caatcttgtt ttctactata cagagcaaga agagtcaggt
     4021 ttaaagggag tcgttaatcg taatgtgacc aaacaaatct atgatttagt tgcttttaag
     4081 gttgaagaga ctgaaaagac cagtctagga aaggttcact taacagaaga tggccaacct
     4141 tttacacttg accaactgtt ttcagatgct agtaaggcta aggaacagct gataaaagag
     4201 ttgacctcct tcatagagga taaaaaaata gagcaagacc agagtgagca gattgtaaaa
     4261 aacttctctg accaagactt gtctgcatgg aattttgatt acaaggatag tcagattatc
     4321 ctttatccaa gtcctgtggt tgaaaattta gaagagatag ccttgccagt atctgctttc
     4381 tttgatgtta tccaatcttc gtacttactc gaaaaagatg cggccttgta ccaatcttac
     4441 tttgataaga aatatcaaaa agttgtcgct ctaacctttg atgatggtcc aaatccagca
     4501 acgaccccgc aggtattaga gaccctagct aaatatgata ttaaagcgac tttctttgtg
     4561 cttgggaaaa atgtttctgg gaatgaggac ttggtgaaga ggataaaatc tgaaggtcat
     4621 gttgttggaa accatagttg gagccatccg attctctcgc aactctctct tgatgaagct
     4681 aaaaagcaga ttactgatac tgaggatgtg ctaactaaag tgctgggttc tagttctaaa
     4741 ctcatgcgtc caccttatgg tgctattaca gatgatattc gcaatagctt ggatttgagc
     4801 tttatcatgt gggatgtgga tagtctggac tggaagagta aaaatgaagc atctattttg
     4861 acagaaattc agcatcaagt agctaacggc tctatcgttt tgatgcatga tattcacagt
     4921 ccgacagtca atgccttgcc aagggtcatt gagtatttga aaaatcaagg ttataccttt
     4981 gtgaccatac cagagatgct caatactcgc ctaaaagctc atgagctgta ctatagtcgt
     5041 gatgaataag caagaaaaaa taggtctgtt agatatttga cagacttatt ttttacagaa
     5101 gatagtacta cttaaaaaat gttttatgct ataattgatg aataaaatag aaggagaagc
     5161 atatgaatac ctatcaatta aataatggag tagaaattcc agtattggga tttggaactt
     5221 ttaaggctaa ggatggagaa gaagcctatc gtgcagtgtt agaagccttg aaggctggtt
     5281 atcgtcatat tgatacggcg gcgatttatc agaatgaaga aagtgttggt caagcaatca
     5341 aagatagcgg agttccacgt gaagaaatgt tcgtaactac caagctttgg aatagtcagc
     5401 aaacctatga gcaaactcgt caagctttgg aaaaatctat agaaaaactg ggcttggatt
     5461 atttggattt gtatttgatt cattggccga acccaaaacc gctcagagaa aatgacgcat
     5521 ggaaaactcg caatgcggaa gtttggagag cgatggaaga cctctatcaa gaagggaaaa
     5581 tccgtgctat cggcgttagc aattttcttc cccatcattt ggatgccttg cttgaaactg
     5641 caactatcgt tcctgcggtc aatcaagttc gcttggcgcc aggtgtgtat caagatcaag
     5701 tcgtagctta ctgtcgtgaa aagggaattt tattggaagc ttgggggcct tttggtcagg
     5761 gagaactatt tgatagcaag caagtgcaag aaatcgcagc aaatcacgga aaatcgattg
     5821 ctcagatagc cttggcctgg agcttggcag aaggattttt accacttcca aaatctgtca
     5881 caacctctcg tattcaagct aatcttgatt gctttggaat tgaactgagt catgaggaga
     5941 gagaaaactt aaaaacgatt gctgttcaat cgggtgctcc acgagttgat gatgtggatt
     6001 tctagaaaat cataaaaaga attgtacatt attctaattt ttgatataat agtcagcagg
     6061 aaagaaagtc ttatggcgtt cttcaagcga gcttgggata gtgggagcca agtagggcaa
     6121 aataatgagc tggcgctttc tgtagtattt tcaaaaacaa tgaagtaata aattagggtg
     6181 gaaccgcgtt tctgacgccc ctaggttaaa tcaacctagg attgtcggac gcggttcttt
     6241 tgcttattca gtctattgtg tgaaagaaag gagagccgtg gacaaccttt atcttgtaaa
     6301 agacgatagt caactagcta catttcgtga ttttgtagta agaaatactg aaaagttgaa
     6361 agattatcaa tcttttttaa agaatgaact tgcagtctgt gatttaccgc aagccgttat
     6421 ttggtcagat tttaatgctg ctacacagat tattagggaa agcgctgttc cagcctatac
     6481 aaataataga cgaatggtta tggcgcctga tttagctgtt tggaaagagt tgtatttgta
     6541 tcaattgatg gactacgagt gttctcagca aactcaagca atagaaagtc actatcattc
     6601 tttatctgaa aatttcctct tacagattgt aggacatgag ttagctcatt ggtcggagca
     6661 ttttttagat gattttgatg gttatgactc ttatatctgg ttcgaagagg ggatggttga
     6721 atatattagt cgcaagtatt tcttgacaga agaggaattt caagcggaaa aaatttgtaa
     6781 tcaatctctc gtagaacttt ttcagaagaa gtatggttgg cattcattga atgattttgg
     6841 ttcttcgact tatgataaga actatgcaag tattttttat gaatactggc gcagcttttt
     6901 gacagtagat aagttggtag aaaatttagg tagtgtacaa gcggtcttag attcttatca
     6961 tttatgggca aatacagaaa aaacttttcc cttgttagat tggtttgttc agcagaaatt
     7021 aattgaaaaa gaaatataaa aactaaagga gtaaacaatg tctaagaaat tgacatttca
     7081 ctgcgtcagt ggcagaaacc tccttacagt cggactgccc cacgctcagc actagagtgc
     7141 ctgagctaga cgcagtacta actcgtcttg cctcgtatga tcgacgaggc agactcatgt
     7201 cgcaagtaat ttatataaaa aggagtaaac aatgtctaag aaattgactt tccaggaaat
     7261 catccttact ttgcaacaat tttggaatga ccaaggttgt atgcttatgc aggcttatga
     7321 taatgaaaaa ggtgcgggaa caatgagtcc ttacactttc cttcgtgcta tcggacctga
     7381 gccatggaat gcagcttatg tagagccatc acgtcgtcct gctgacggtc gttatgggga
     7441 aaaccctaac cgtctctacc aacaccacca attccaggtg gtcatgaagc cttctccatc
     7501 aaatatccaa gaactttacc ttgaatcttt ggaaaaattg ggaatcaatc ctttggagca
     7561 cgatattcgt tttgttgagg acaactggga aaacccatca actggttcag ctggtcttgg
     7621 ttgggaagtt tggcttgacg gaatggaaat cactcagttc acttatttcc aacaagtcgg
     7681 tggattggca actggccctg tgactgcgga agttacctat ggtttggagc gcttggcttc
     7741 ttacattcaa gaagtagact ctgtctatga tatcgagtgg gctgatggtg taaaatacgg
     7801 agaaatcttt atccagcctg agtatgagca ctcaaaatat tcatttgaaa tttcggacca
     7861 agaaatgttg cttgaaaact ttgataagtt tgaaaaagaa gctggtcgtg cattagaaga
     7921 aggcttggta caccctgcct atgactatgt tctcaaatgt tcacatacct ttaatctgct
     7981 tgacgcgcgt ggtgccgtat ctgtaacaga gcgtgcaggc tatatcgctc gtatccgtaa
     8041 cttggcccgt gtcgtagcca aaacctttgt cgcagaacgc aaacgcctag gctacccact
     8101 tttggatgaa gaaacaagag ctaaactcct agcagaagac gcagaataaa gagagtgaca
     8161 aattacgaaa atgggcgaac agagtgagcc ctgagccagt tgccgcagtg atgaaggtat
     8221 ccttagtgaa actaaggata ctaggcaaaa ttggagactt ttggctccaa ttttagcaat
     8281 gaaacaacga agttggttgc ttgcgtgcca atcacataag gcaaactgga aaataaaaag
     8341 atacttttcg gagaaaaaac atgacaaaaa acttattagt agaactcggt cttgaagaat
     8401 taccagccta tgttgtcaca ccaagtgaaa aacaactagg cgaaaaaatg gcagccttcc
     8461 tcaaggaaaa ccgcctgtct tttgaagcca ttcaaacctt ctcaacacca cgtcgtttgg
     8521 ctgttcgtgt gactggttta tcagacaaac agtctgattt gacagaagat ttcaagggac
     8581 cagcaaagaa aattgccttg gatagtgatg gaaacttcac caaagcagct caaggatttg
     8641 tccgtgggaa aggtttgact gttgaagata tcgaattccg tgaaattaag ggtgaagaat
     8701 atgtctatgt cactaaggaa gaagttggcc aatcagttga agccattgtt cctggtgttg
     8761 tagatgtctt gaagtcattg actttccctg tcagcatgca ctgggctgga aatagctttg
     8821 aatacatccg ccctgttcac actttaactg ttctcttgga tgagcaagag tttgacttgg
     8881 atttccttga tatcaaggga agtcgtgtga gccgtggcca tcgtttcttg gggaaagaaa
     8941 ccaaaattca gtcagcattg agctatgaag aagaccttcg taagcagttt gtaatcgcag
     9001 atccatgtga acgtgagcaa atgattgttg accaaatcaa ggaaattgag gcaaaacatg
     9061 gtgtacgtat cgaaattgat gcggatttgc tgaatgaagt cttgaatttg gttgaatacc
     9121 caactgcctt catgggaagt tttgatgcta aataccttga agttccagaa gaagtcttgg
     9181 tgacttctat gaaggaacac cagcgttact ttgttgttcg tgatcaagat ggaaaactct
     9241 tgccaaactt catttctgtt cgtaacggaa acgcagagcg tttgaaaaat gtcatcaaag
     9301 gaaatgaaaa agtcttagta gcccgcttgg aagacggaga attcttctgg cgtgaagacc
     9361 aaaaattggt gatttcagat cttgttgaaa aattaaacaa tgtcaccttc catgagaaga
     9421 ttggttccct tcgtgaacac atgattcgta cgggtcaaat cactgtactt ttggcagaaa
     9481 aagctggttt gtcagtggat gaaacagttg accttgctcg tgcagcagcc atttacaagt
     9541 ttgacttgtt gacaggtatg gttggtgaat ttgacgaact ccaaggaatt atgggtgaaa
     9601 aatacaccct tcttgctggt gaaactccag cggtggcagc tgctattcgt gaacactaca
     9661 tgcctacatc agctgaagga gaacttccag agagcaaggt cggcgcagtt ctagccattg
     9721 cagacaaatt ggatacgatt ttgagtttct tctcagtagg attgattcca tcaggttcta
     9781 atgaccctta tgcccttcgt cgtgcaactc aaggtgtggt tcgtatcttg gatgcctttg
     9841 gttggcacat tgctatggat gagctgattg atagccttta tgcattgaaa tttgacagtt
     9901 tgacttatga aaataaagca gaggttatgg actttatcaa ggctcgtgtt gataagatga
     9961 tgggctctac tccaaaagat atcaaggaag cagttcttgc aggttcaaac tttgttgtgg
    10021 cagatatgtt ggaagcagca agtgctctcg tagaagtaag caaggaagaa gattttaaac
    10081 catctgttga atcactttct cgtgccttta acctggctga gaaggcagaa ggggttgcta
    10141 cagttgattc agcactattt gagaatgacc aagaaaaagc tttggcagaa gcagtagaaa
    10201 cactcgtttt atcaggacct gcaagtcagc aattgaaaca actctttgcg cttagcccag
    10261 tcattgatgc tttctttgaa aatactatgg taatggctga agatcaggct gtccgtcaaa
    10321 atcgtttggc aatcttgtca caactaacca agaaagcagc taagtttgct tgttttaacc
    10381 aaattaacac taaataaaat ttgataaacg gactttatct tattacaaag gagaagaaat
    10441 ggatccgaaa aaaattgctc gtatcaatga gcttgctaaa aagaaaaaaa cagaaggctt
    10501 aacaccagaa gaaaaagtgg aacaagccaa actacgtgag gagtacatcg aaggttatcg
    10561 ccgcgctgtt cgtcaccaca ttgaaggaat caaaattgtg gacgaagaag gaaacgatgt
    10621 tacaccagaa aaactacgcc aagtacaacg tgaaaaagga ttacatggcc gtagtcttga
    10681 tgatccaaat tcataataat actcttcgaa aatcaaattc aaaccacgtc agcttcacct
    10741 tgccgtactt aagtacagcc tgcggctagc ttcctagttt gctctttgat tttcattgag
    10801 tatatgtatt ctttctttta acaaagatag atgaaacgat aacaaagaga ctagcagttt
    10861 gtgtttgcta gtctttttt
//
LOCUS       DAVKQB010000061        10758 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKQB010000061 DAVKQB010000000
VERSION     DAVKQB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.061"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(117..1541)
                     /locus_tag="VW528_001816"
     CDS             complement(117..1541)
                     /locus_tag="VW528_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9912594.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVAEKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VW528_001817"
     CDS             complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VW528_001817"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9912595.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPYGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VW528_001818"
     CDS             complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VW528_001818"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9912596.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERGNLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6528..7706)
                     /locus_tag="VW528_001819"
     CDS             complement(6528..7706)
                     /locus_tag="VW528_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9912597.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGFIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSIHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7786..8649)
                     /locus_tag="VW528_001820"
     CDS             complement(7786..8649)
                     /locus_tag="VW528_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9912598.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYFFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8652..10553)
                     /locus_tag="VW528_001821"
     CDS             complement(8652..10553)
                     /locus_tag="VW528_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9912599.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGDELDKLMSDYDHLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSKKTSGNVVLTVENTAI
                     GYDGEVLSQPINVDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYIEKRATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3232 a         2368 c         1909 g         3249 t
ORIGIN      
        1 tgtactattc tagtttcaat atactataat accaaaaagt tcgtattttt tctataatta
       61 ttattatttt atagcaaaaa aataaaacca ggaaaacaat ttcctgattt tacacgttat
      121 ttcacatcaa aaacaatgga tttgacattt gtcatcgctt caatgctata tttaattcct
      181 tgcactccag ctccagaacc tttgacacca aggaatggga aattatctgg accacgctgg
      241 gttttattat taatgtggac tgtacctact tcaagttttt cagcaatttc aaaggctttt
      301 ttgaaatcat ttgtaaagac tgatgattga aggccgaatt cagattcgtt ggcaaaggca
      361 atagcttcct ctacactagc cacacgaatg attggtaaaa caggaccaaa tggctcttcc
      421 catgccactt tcatatcttt tgtaacttgg tcaaaaagca ctggccagag gagattgccc
      481 tcacgtttga ttggtgtaag agcctgagcc tctttttctt gtgcatcctc aatcaagccc
      541 caaatgaagt cggctgaagc attgtcaata acaggtgtaa tatcagcatt gtcaaatgga
      601 tcaccaactg ttaatttaga aacttcttcc tgaagcaaag tagctaattt ctctgctaca
      661 ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt gtcctgagta gctaaaggct
      721 cccgcaacaa tttgcttggc agcatgttcc aaatctgcat cttctagtac aagagctgca
      781 tctttcccac caagttccaa catgatagga cgcataccag ctaaacgacc aatacgttct
      841 ccaataggag ttgaacctgt aaagttgatg aagttgactt ctttgtgctc aatgatataa
      901 tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa cacctgccgg aatccctgct
      961 tcttcaaatg ctttagccaa caagagtcca gaaatggaac cttgtgttgg tggcttaaac
     1021 atgaccacat tccctgcaat caaggcaggt gcaattttag aagcagataa attaactgga
     1081 taattaaagg gagcaatcgc tagcacgata ccaactggtt cacgacggac aacagccagt
     1141 ttgtttttac ttgctgcctc aaaaccacca ccttccattg cttgtccagt gatacggaga
     1201 ccttcctcag cagcataacg aatcaagtct gctgtacgca ctacttctcc aattgctgct
     1261 ttaatccctt ttgctacttc tttggcaagg atagtaccaa tttcttcctt atcgcgttct
     1321 aaaatagctg ctgttttatg taaataagcc gcacgttcaa ctgctgataa agctcgccat
     1381 gctggcaggg ctgcacgcgc agcttgcata gcctcatcag cttcagtctg agtcatggct
     1441 ggaactgtac ccaattcttc ttgattgatt ggtgaataaa tcgtaatttc ttgttcagat
     1501 gatttccatt ttccatttac taaattctga tatcttgtca aattcttgtc ctcctgaaaa
     1561 tgattaagat atattttacc aaattttcag aaaatcacaa ccctttttgc aaaatttttg
     1621 agattatttt cacaaacttg atttttcaaa gtatactcaa taaaaataaa aaaaatccac
     1681 tacgtcaagg cgaggctaat gtggtttgaa gaaattttcg aagagcgtga atgagtatca
     1741 tctatagtaa aataaaaaaa ctgaacaatt tggttgggga cagccaaacc aatttctcac
     1801 aatgtttcag aaacaagggt gtgctactcc aatttcagcc tactataact gtcatagatt
     1861 gctgaaacaa agtctaggta aaagtcttca taataaaaag acctcctatc aagtgttcaa
     1921 aaactttgat aggaggtctt gttttgtgaa aatatttatc aaattttcta tacaagtgag
     1981 ctgttagcca ggttctttct attctttcaa tttcaatgaa tggatttttt actaatactc
     2041 ataactggga atttgtctgt gtaaaaatag cgagatagat ggtatttata aaacactcaa
     2101 gacagctaga ctaatatcat ttaaaacatt atcttctttt gagcgactgt tggttaccaa
     2161 catagctaaa tttcctgcat tttcaaattg atagggttct gatttagcat tcacaaccac
     2221 caagaggtgt tctttgccgt gaacttcata gataaggtag ccgctatgtt caatcgcaga
     2281 atgcacaaag acatgatggt aaatttcatc atagctagag taagaaaagg caccagtttt
     2341 tgtcttcaat cggatgactt gacggataaa ctcaatactg tcttgacgct cattaatcaa
     2401 gttccagttc acttggttca cactgtcagg agcattatag ctattcatcg cacgctctct
     2461 atcatcatgg gtcaactcac cattttcacc agtcgcaacc agtttggtac gaccaaattc
     2521 ttgaccgatt tccataaagg ccatcccctg catgagcaga ttcatggctg tggcagtttc
     2581 gaccttgcgc atgatttgct ctgaactttg gtctggatga agggttgcca ataaatcgtg
     2641 aagattgtaa ttgtcatggg cttctacata gttaagcacc tgatttggat gtgtatagct
     2701 tcctaattca cgacttccta ggattgcttt agctagaatt ggctctgtcg cagcaccact
     2761 gacaaaacct gacttgatag caccataaac ttctccccct ttgacagcat cgcgctgatt
     2821 gtcattaaag aaaccaatat ttggcatctg gtaggcattg tccttcttgg ccttatcata
     2881 aggggcaaga cctgttccca tatcccatcc ttctccatag aggataatgt tggagtcgat
     2941 ttcatccaag ctttgacgaa tcatctgcat ggtcttgaca tcatgaatcc ccatcaagtc
     3001 aaaacggaag ccgtcaatat tatattcctg cacccagtat agaagagaat caatcatata
     3061 cttgcgaaac atttcgtgtt cactggctgt ttcatttcca acacccgttc cattctggaa
     3121 ggtaccatat ggattcatac gataatagta atcagggact gttgtttgga atggtgcatc
     3181 aacaactgag aaggtatggt tatagactac atccataatg actccaatac ccgcatcgtg
     3241 ataagcttga accatcacct tcaaatcacg aatgacctga gctggatcat ctggattagt
     3301 tgaaaaacta gtttctggcg cgttatagtt ttgtggatca taaccccagt tgtaggttac
     3361 atttccatcc tcatcgtatt ctttatgacg gtctgcaatt ggttgcaatt gaacataatt
     3421 gtagcccagc ttcttgatgt aatcaaaagc agttgactgg ccgtattggt taactgttcc
     3481 agcctgagca gcacccaaga aagttcctcg aagatgttca tctacacccg atgtaggtga
     3541 tttagtcaaa tcacgaatat gcatttcaca gataactgcc ttacatggat tttccaagcg
     3601 ccaagtagcc tccgaaccgt gcttaacctc gaagttttca acttgctttt ctacatggct
     3661 cagaatagct gaacgtttgc catcagggct ggtcgcgatt gtataaggat cacgtgtcag
     3721 tgtttggtga tgagggaatt ggacttgata ctgataagcc ttacctacca aatcttcctc
     3781 aacatccaaa ctccagacac cgattgtatt gtccttatga ttataagagt agctattgcc
     3841 tcttttcatc tcaaaagtct tccaaacggg tgcatcatta gcagctgatt cataaacgac
     3901 aacttgcact tctgtcgctg taggtgacca gagggaaaaa tgagcctgat tgtcctctac
     3961 acggcaaccc aattctcctt ggtaacccca atgatggtca aaactagcac tgttaatggc
     4021 cttatcaaag gcaaaaggat tttgattttt atagaaagga ctggcaatag caggattttc
     4081 agagtaataa atcctatcat cgccttccaa aatccagacc tctgttaata ggggatagtg
     4141 attaaaacgg atagaatatt ctttactagt ttgacctgta tgaaccacaa aattcaagct
     4201 ttctataaca tgtgaacttg ggtgttcaaa gctaaataaa gctccaaaat aatcttcttt
     4261 gtaggttagc aaatcaattc gttgatcctg actttttaca aaggagcaag tgtcatattc
     4321 tccattctta cgatggtaat gaatgcgcat agggtagtta tacatttttt atttttcctt
     4381 tttactttgt ttctatttca ctaataaatt tttgtcaatc tcgtctcaat taacagacat
     4441 agtcatattc tctaaactct gtttttaaac gatccattac aaactttcta gccatgcctc
     4501 atctctgacc tggataccaa gttcttgtgc tttttgcagt ttacttccag cgtctgcacc
     4561 taccacgacg aggtcggtct ttttagaaac actacctgtc actttggcac ccagactttc
     4621 gagtttactt ttagcttctg agcgcttgag tcgttccaat tttcctgtca ataccacggt
     4681 caaacctgac aaggccgcat ccgctactac cgtctgtcct ttatagtcca gattgacccc
     4741 agtttctttc aattctctga gcagaatttc agagccttct gtcgcaaaat aagtctgaag
     4801 acttttggca atcacgccac ctagactttc aatactagcc acttcctctg aatctgcctg
     4861 atacagattt tcaattgaat ggaaatattg aagtaaaagc tgactagcct tgcttccgac
     4921 atgacgaatt cccaaaccaa ataagagctt ctcggcagaa ttttcctttg atgcttggat
     4981 agcctgatac agtttagcag cggacttttc cttaaccccc tctaaaagga ggaaatcctc
     5041 ttcttgcaaa cgataaatat ccgccacatc cttgactaaa ttagcagcaa aaagcttctc
     5101 aacaatagat ggaccaagac ctgtaatatt catagcatca cgagaagcaa agtgaatcaa
     5161 gccttccatg atttgagcag ggcaacgcgg attgatacaa cgtagggcca cttcatcttc
     5221 aaagtgcaac aagtcagagt tacaacttgg acagtttgta gggatatcta gtttttcttc
     5281 agaaacccgt ttggactcta ccacacgtaa aacggcaggg atgatgtcac cagccttata
     5341 tacaatgacc gtatcgtctt ttcggatatc tttttcagca atataatcta cattgtgcag
     5401 ggtcgcacgg ctaacagtcg taccggcaag ttgtactggt gttagattag cagttggagt
     5461 tacaacaccg gtacggccaa ctgtccagtc aactgataag agttgagctt ctttttcttc
     5521 agcagggaac ttgtaggcta ctgcccactt tggagcctta acggtaaaac caagttcttc
     5581 ttgacttgct aggtcgttga ccttgattac cactccatca atatcgtaag gcagatttcc
     5641 ccgttcctgt cctacttctt ggataaaatt ccagatttca tctatgtttt cagccaagat
     5701 tcgcttagga ttgaccacaa aacctagttg ttctaggtac ttcaaaccct tttcttggct
     5761 atcacgagtt gaagggctgg cttcttgata gagaaacgtt gcaagattac gcttggcaac
     5821 tactgctgta tccaactgac gcagagttcc tgctgccgca ttacgaggat tagcaaattc
     5881 aggctctcca ttttcttggc gcacttggtt aacttggtca aaggaagcgc gtggcatgta
     5941 acattcccca cgaactgtga tatctagttc ttctggcaaa gtcaaaggga tgtccttaac
     6001 acgcttgagg ttttctgtga tattttcacc aattgaacca tctccacgtg ttaccccagc
     6061 aaccaaaatc cccttttcat aagtcagcga gatagataag ccatcgattt tcagctcaca
     6121 aatataggtc ggatgagcca cttccttacg aacacgcgca tcaaaagcat ctagctcctc
     6181 acgtgaaaaa gcatcctgca aactataaag aggatactga tgactgtatt tttcaaaacc
     6241 atctaaaacc ttgccaccaa cacgatgagt cggactgtct gctagcactt gctctggata
     6301 agcagtttct aactcgacca actcacggta aaggcggtca tactcactgt ctgaaaccga
     6361 gggattatcg ctggtatagt actcagtcgc atagcgattg agcaaagcga ctaactcatt
     6421 cattctttta ttcataagac cattttacca taaaacaagc cctcctcaca aacgagaagg
     6481 gcggaaaaaa cacttagttt gaaattattt ttgaaactca agcaacctta tatcaatttt
     6541 tcaaaatgag ttcgaacata aataaacgat atacaagaca agatgataac accacttcca
     6601 attatcagga aagaagagag atgtatactt ggcaagactg tcataaatcc ttttgcaata
     6661 ggcataaata gaatagctaa ggtaaaaatt gtactcagta ctcttccaag aaattcgctc
     6721 tcaaccttgg tttgtacttg agtaaaaaag tgaatattaa aaatcgtcat aaacaattca
     6781 caaactaaat ttccagaaaa ggaaagaaaa gttggaagtg gtaatcccat cataaaaact
     6841 ccgacacctg tcaaagccag taaaatcaaa agattataaa tattagcttt aattttacta
     6901 gctagaagag ccccaatgat gaaaccaata gcccccatag ttaaaatact tgcataggct
     6961 ccttctgacc cgtaaagctg attcgaaaag ggaagtagaa attcaaaagc tgcaaaaaag
     7021 aaattaacgc tggaagctac cagcaaaagg aagaaaattt cttgctgatg ccagatatag
     7081 tgtaacccat ccttgatatc tacaaaaata tctctcccag taaaagcctt tttctcttga
     7141 acttttgctt cctcttttgg aaggaaagcc actagaacaa aagcaatgaa aaaagtcagc
     7201 gagtctagca gtagcgtcat atggagactt gcaaactgta aaacaaggaa ggaaagaaca
     7261 ggagagctaa cacctacaac ctgcaaaacc agctctaagc gagaattata gatcacaatc
     7321 tcatctttct ccaccacttc agttatgata gctttattgg ctgtgcgaga aaaggcaaaa
     7381 gcaatagcct gcacaatgtt agcaacaatc aaagcgccaa tcatccagct atcattcctt
     7441 atgaaagaaa tagccagaca aagaatccca caaacaagat ctgccgtcat taaaatctta
     7501 cgacgagaaa aacggtctga aataactccg ccaaagggat tgacgagaat agatgtgacg
     7561 agctcagaaa tctgatacat tcctaaaact gtctgtccta tagtccccat agaagccaac
     7621 cagacactat ttccataatc atagagcata tttcccattt tattgatagc cccacggcta
     7681 atcaactgca ctgcatagcg attcatatta aagctcctct caaattttga aactattgta
     7741 tcaaaaccga aaggagcttt ttattttttc ccttatttgg gaaaattaac ttttgacaaa
     7801 tttttcgtag tgttcctgat aataggctac ttgctctgga agacctaaca catcaaaaat
     7861 atgtatggcc tcttgcatct gcttacagcc ttctttacac tgtccttttt gatataaggc
     7921 aaaacctttt aaataatgga aaacattacg ctcataaagc ttaatacctt tgtcaataat
     7981 cttctctgta taagcctcaa aatagttggc attataaaaa gaagaatgct ctaaacaatg
     8041 ctggtaacaa ttgagggcca aaatcaaaac taatctctta tggcgactaa tctcttggta
     8101 aaattcctcc ctctccataa cttctctacc aatccgagtg acatagtcta catcgtagaa
     8161 actatagagg ttaccgaaaa gaatcaactc atacatggtc cattcttctg ttttgaagaa
     8221 ataatctgct accttatcca aatcatcctg cttcatatca taactcgcat ctctttgaca
     8281 aatcagacct tgtagcaaaa tccagttcag ctcaaaataa aggggagtcg tcgaactctt
     8341 agacttttca agttgttctc tttgaagctt ttgaaaacct gcaatatcgt ttgaatagta
     8401 aagtgggata atctgtgcca tcatagacac atgttcatga ttatgaaaat tccttgcctt
     8461 atccatgaaa ttttcgattg ttacatgaat gttatccaaa atctcaaaga aacgggagac
     8521 tgccaggtca gactccccaa gctcaaagcg agataactga gaggtagagc aggattcgcc
     8581 tgctgcttcc tttaaagaat aatttccact tgttcgaaat tcacgaaata cttttccaag
     8641 atgttccatc tttacacctg atctgataat tcttcccact caagcatagc ttcttcctga
     8701 cgatggctga ttttgtccag ctcagcctgt aattccatga gtttgtcggc atcgtttgtt
     8761 tccaacattt gttcagaaat ggcttggctt tgactttcta gctcttcaat ttcagcttct
     8821 agactttcga tttgtcgcat gagtttgcga acttcttttt gactttcttt ctgggcctga
     8881 tagtcattga ctggacttgc ttcctttgct tgattgctag ttgaagcttc ctcagtctga
     8941 ctcatttctg ctgttgctct cttctcaata tagtagtcgt aatctccaag gtagagagtt
     9001 gaaccattct cagacaattc caaaacatga gttgccacac gattgataaa gtaacgatca
     9061 tgactgacaa aaagcaaggt accatcaaag tcaatcaagg cattttctag cacttcctta
     9121 ctatcaatat ccaagtggtt ggtcggctca tccagaatca aaaagttatt gttttccata
     9181 gacaatttag ctaaaagcaa acgagctttt tcgccaccag atagcatgcc gactgatttt
     9241 ttaacatcat ctcctgagaa aaggaaggct ccaagacggt tgcggatttc aacttctggt
     9301 gtcagtttga aatcattcca gagttcatcc agcaccgtat tacttggtgt cagcttgctt
     9361 tgggtttggt catagtaacc aacctcaaca ttagcgccaa agcgcttttc tcccttgata
     9421 aaaggaatct ggtccacaat agacttgata aaggttgact tgccgatacc atttggacca
     9481 acgatagcga cagcattcat cttacgaaga tctacgttaa tcggttgtga caggacttcc
     9541 ccgtcataac caatagcagt attttcaaca gtcaaaacaa cattgcccga cgttttttta
     9601 gactggaagg tcatgttggc tgctttcttg ccagcttcag gcttgtccaa acgctccatt
     9661 ttttctagtt gtttacggcg agattgagca cgtttagtcg ttgaagcacg aactagattg
     9721 cgattgacaa agtcttccag agcagcgatt tccttctgtt gcttttcata gttttttgcc
     9781 tcagtagcta gcttttgctc cttcaattcg acaaaacgag agtaattccc cacatagcga
     9841 tccaaggaat gcttggtcaa atctagcgta attgtcgcaa ccttgtccaa gaaataacgg
     9901 tcgtggctga cgataatgag ggcaccgcta tagtttacca agtaattctc tagccaggcg
     9961 atggtttcaa tatccaagtg gttagttggc tcgtccaaga ccaagagatt gggcttttca
    10021 aggagcattt tagctagagc caaacgagta ttttgaccac ctgaaagttc agctattttc
    10081 atctgccaca tagactcgtc aaacttgaat ccattcaaaa tcgctcgaat atcagcttca
    10141 taggtaaagc cgcctgcttg gcgaaaattc tcagataagt ggtcgtaatc tgacatcagt
    10201 ttatccaatt catcgccaga cttttcaccc atctccaact ccatctgacg aagttgttta
    10261 tctgtccgac gtaagtcatc aaagacatga agcatctcat catagatggt attttcagac
    10321 tcaaaacggc tatcttgagc taggtaagac agagaaatat cttttttctt attgatttct
    10381 ccactagttg gctcctcttc tccaactaaa atcttcaaaa gagtagactt acctgcacca
    10441 tttttcccaa caagggcaat ccgatctcgt tcatcaacct gcaggttgat attatcgaaa
    10501 agaacctctc ctgcaaaaga acgttcaatt ttattagctt gtaaaataat catacaagta
    10561 gtatagcatg tttccctaag tcattcaaga taatcgtaag tcttttagta caacttttat
    10621 aacataaaat aaactaaatt atgtatattt tatattagat tccttcacta tcttgttgga
    10681 ttttctaacc agctaatctt gtttcaaata gttatcgcac aagcctatta tttaattctt
    10741 ttcattattt acgtacgc
//
LOCUS       DAVKQB010000062        10651 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKQB010000062 DAVKQB010000000
VERSION     DAVKQB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.062"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..614
                     /locus_tag="VW528_001822"
                     /pseudo
     CDS             1..614
                     /locus_tag="VW528_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(742..1764)
                     /gene="cbpF"
                     /locus_tag="VW528_001823"
                     /pseudo
     CDS             complement(742..1764)
                     /gene="cbpF"
                     /locus_tag="VW528_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(1783..2604)
                     /gene="cbpG"
                     /locus_tag="VW528_001824"
     CDS             complement(1783..2604)
                     /gene="cbpG"
                     /locus_tag="VW528_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEU9912600.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2685..2924)
                     /locus_tag="VW528_001825"
     CDS             complement(2685..2924)
                     /locus_tag="VW528_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912601.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3074..3340)
                     /locus_tag="VW528_001826"
                     /pseudo
     CDS             complement(3074..3340)
                     /locus_tag="VW528_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3365..3676)
                     /locus_tag="VW528_001827"
     CDS             complement(3365..3676)
                     /locus_tag="VW528_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9912602.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLT
                     S"
     gene            complement(3601..3843)
                     /locus_tag="VW528_001828"
     CDS             complement(3601..3843)
                     /locus_tag="VW528_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9912603.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIFQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(3844..4476)
                     /locus_tag="VW528_001829"
     CDS             complement(3844..4476)
                     /locus_tag="VW528_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9912604.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(4490..5485)
                     /locus_tag="VW528_001830"
     CDS             complement(4490..5485)
                     /locus_tag="VW528_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9912605.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDQDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5482..6180)
                     /locus_tag="VW528_001831"
     CDS             complement(5482..6180)
                     /locus_tag="VW528_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9912606.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6258..7268)
                     /locus_tag="VW528_001832"
     CDS             complement(6258..7268)
                     /locus_tag="VW528_001832"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9912607.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLASGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(7252..8259)
                     /locus_tag="VW528_001833"
     CDS             complement(7252..8259)
                     /locus_tag="VW528_001833"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9912608.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAESTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8246..9199)
                     /gene="mvaD"
                     /locus_tag="VW528_001834"
     CDS             complement(8246..9199)
                     /gene="mvaD"
                     /locus_tag="VW528_001834"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9912609.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(9181..10059)
                     /gene="mvk"
                     /locus_tag="VW528_001835"
     CDS             complement(9181..10059)
                     /gene="mvk"
                     /locus_tag="VW528_001835"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9912610.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10234..10512)
                     /locus_tag="VW528_001836"
     CDS             complement(10234..10512)
                     /locus_tag="VW528_001836"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9912611.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSAQVPWQ"
BASE COUNT         3146 a         2469 c         1880 g         3156 t
ORIGIN      
        1 aaaccagata aggaccaaga gcttaaagct gaaattcaat ccatttttat cgaacacaag
       61 ggaaattata cttatcgccg ggttcattta gaactaagaa atcgtggtta tctggtaaat
      121 cataaaagag ttcaaggctt gatgaaagta ctcaatttac aagctaaaat gcgacagaaa
      181 cgaaaataaa ggagacgttg gtaagaaggc agagaatctc attcaaggcc aatttgaagg
      241 ctctaaaaca atggaaaagt gctacacaga tgtgacagaa tttgccattc cagcaagtac
      301 tcaaaagctt tacttatcac cagttttaga tggctttaac agtgaaatta tcgcctataa
      361 tctttcaact tcacccaact tagaacaagt acaaacaatg ttggaacagg cattcacaga
      421 gaagcactat ggtggagtcc ttctttggga ttctgaaatc ggaaatgttt tatggctatg
      481 agaaaacatt taaatcactt aatcaattgg aacaagctat tatagactat attgattatt
      541 acaacaacaa gagaattaag gtaaaactaa aaggacttag tcctgtgcag tacagaacta
      601 aatcctttgg ataaattatt tgtctaactt ttggggggca gtacagaaaa accttgatac
      661 tatgcgtttt ttgattgtga agacttttat ccacctatta tacaaagtta agtatccatc
      721 acagtttaca attagccttc attacttaac ccattcacca ttatagttta agtagtaacc
      781 acctactgtg gtattaacag ctaaagcacc tgaatcatag gcatagtacc agttaccatt
      841 gacttggaac cagcctgttt tcatatctcc attacttgca tttagatagt accaagtcga
      901 accttcctga taccaaccag ttgccatagc tcctgatgaa cggagatagt accacttgtt
      961 accaaggtat tgccagccag ttctcatatc accattttca gcatctagat agtaccaagt
     1021 tgagccttcc ttataccagc cagttgccat agctcctgac gaatggaggt agtaccatct
     1081 attacctaga aattgccaac cagtttgcat aatgccagtt gttggatcta gatagtacca
     1141 tggagctgct tttagcttct cttcatcata ggtaagaggg gaatccttaa cccaaccacg
     1201 tgctagctct ccaaccgcca atctgttgat gcgcgaatca aagccaccat ccttctgtaa
     1261 ataataccaa tgaccctctt cgtaaatcca accagttttt aaagtatgat aactacgctg
     1321 atcttcaaaa taatagactc atttctctgc tgggctatca tattcttccc catgatgttt
     1381 gttggtattc gtagcagttt ttgcttctaa aacttgcttg ccaacaaatt cttgtaatac
     1441 accatcttga ccaaaataaa accaatctgg tctaagagaa atctctattc ttggagaagg
     1501 accaatcgta acccccttgt gtggagcagg tatatattgc cagccgacaa ccatctctcc
     1561 ggataaagga tcaaaataat agtacttccc atctatcact ctccagtagg tttcttttag
     1621 atcccccttt ttgtagtagg ttctaccatt ttcttggaca aactgccatc cttcagaatc
     1681 atctgcaaat actgtactgg tccctagcaa accgaagaaa aatgtggcta gtgcgacttg
     1741 catcattttt ttcaaaagct tcattttata tatcctccaa cattaaatcc actcaccaga
     1801 tgaagcgaag ttataaactt taccatcgat agtttggcta cctgtaacca ttgctccatt
     1861 tgaattgaga taataccatt ttccatttac tttttgccaa tctgtaagca tcttaccgga
     1921 actgtctaaa taataccaag tatcatttat ctcctgccaa cccgtttgtt tatcatgttg
     1981 tctataatgg taccaactac catttatttt cttccatcct tcaagagagt aacctttaag
     2041 aaccgaataa ataaatggca aatttcgttc atttaattta actgcactgt taatttgatt
     2101 agctccatct cctaaagtat gcactcctac tacacggtga ctagcatcat aaactgtaga
     2161 tccactagac ccctctaaag tatcaacttg gtaatccaag aacatgccat catcacttaa
     2221 aacttgtttt ttatctgtat acatttgatg aattttaaaa ttatatgatg gatagcctgt
     2281 gatagtcaca gttattcctg tcaaattttt ttgactagta ggaagaccca aagtccctaa
     2341 ttttgcacca atgggctctt ctagaattaa taaagccaag tcatattccc ttgcatcctt
     2401 agaatttaaa tttctaaatt ccttcaaata acgaacttcc tttactttga tctttccaaa
     2461 tagttcttga cttggactaa cagccggaag aacataaata tcatccgctt ctttcccata
     2521 gtcatgtctg tagtagttgt gagccgctgt aattaacaca ttatctttaa taaaattcgc
     2581 acttcccctc ccttctacat tcattccatc ggctactcca taatacttag ataaaaccat
     2641 agctgaagtc gaatacggat actgtaaagt attatcaatt ttaatcaaat catcattacc
     2701 gataatactt ctgattgctt ttggtagtat gaaccatacg ttggtgaaat ctcagataat
     2761 gaagaatcat tagatcctgg acctttttct agtgtctcac ttacctcata ttcttcaccc
     2821 gtactagaaa taacactcaa agcagatact gtcgataact ggctagccaa taaagtactc
     2881 gcaataattg aaatacccaa ttttttataa acagttttct tcattattgt agcctcctaa
     2941 tgtaattata gcctactatt ctaaatttct taatctacta tagaatcaag aaatctacca
     3001 ccttctttaa ataccctcca ttatcacata aacaggtaaa cttttcaatt aatgactgcg
     3061 cttttcaatc acgctagagg tacttgcttg cttctttgat actaagttca gccattcttt
     3121 ccttgttttc atcaataaag catgttaccc aagttggatt cgttttggag tagtttcgca
     3181 gaatccagcc aatggcttta ttgataaaaa attctgtttg gttcaagtta tgaagtagaa
     3241 tcttttccat taattgagta ttggtcttct cttttcttaa caactggtgg tcaatagcga
     3301 cacgtctcag ccagatatta tctgataggc tccattttat actcaatgaa aatcaaagag
     3361 caaactagga agttagccgc agattgctca aaacactgtt ttgaggttgc agatagagct
     3421 gacgtggttt gaagagattt tcgaagagta ttaagattat ttcttctagt tcagggtgtt
     3481 catacaccaa actccctact actcgatcta ggatatctac cgtgtcccac caggattttg
     3541 tcacgactaa ctgctctagc ttaggcaaat cggtttcctt tagataagac tgcattgctt
     3601 tcaaatagtt agcagccaca tattggtatt ttctaggctc cttttcccag caagtgtctg
     3661 caaaatccca atcgataatc tttgtttttt tcgcttctgg aaaatatttt tatagagttt
     3721 atttctttca ggcaccgcaa tacctagaaa agaaaattga tggcgcatat aggcttccat
     3781 ggaccttgct tttttagagt cttttgctgc ttctagctcc tcaagtaaat ctgctaaact
     3841 catctaaaac tcctcttgcc ccaccaaatg gtgctgaaag gcatagacag ccgcctgggt
     3901 acgatcgctg acttcaagtt tggcaagaat attggacacg tgggtcttga ccgtcttgag
     3961 agagataaag aggtcatctg cgatgcgctg attttcgtag cccttggcga tgagttggag
     4021 aacatctcgc tcacgcgcag tcaattcttc atgaagttcc atatgattgc ggtggtattc
     4081 aaccttcttg ctaacctctt gctcaatggc cagctcgcca gcagctacct tactgacggc
     4141 atgaagcaat tcatctgcac tagaagtctt gagcatatag cctttggcac cagcatctaa
     4201 gactggcatg attttttcat tgtccaaata agaggtcaca atcaaaatct tggcttcagg
     4261 ccattcttta aggattgcta aggtcgcgtc aatcccattc atctcaggca tgacaatatc
     4321 catgacaatg acatctggac gcagttccaa ggccaagtca atcccttgag acccgttgga
     4381 cgcctcaccc acaacttcta catcgtcttg gaggtcaaag tagcttttca aacccaaacg
     4441 gaccatttca tggtcatcta ctagtaaaat tttcatcttt actcctttat cattccttat
     4501 ctaacagggg aatacggata tcaactgcca gcccttgctt gggagctgtt aataactgaa
     4561 ccgtccctgc catatcttca acccgctcct tgatatttcg cagtccataa ctcaagtcgt
     4621 ctaagctccc taactggaaa ccaatcccat tgtccaccac cttcagttgc aattcaacat
     4681 ctgtctgata gaggtagaca tctaggcaag atgcctgggc atggcggagg gtattgctaa
     4741 tcaactcttg caggatacgg aagatatgct cctcgatttt cttaggcaat ttcgtcatat
     4801 tctgcttgag actaacccta agatcactct tgtcctcaag ctcttttaag agaatttgaa
     4861 tcccttctat caagctcttc tgctccagtt caactggtcg caaatgcaag agcaaaaccc
     4921 gcaaatcctt ctgggctgtt tctaaaatag ctgtgacact ctgcaactgg gtctgcatct
     4981 tttctctatc caatttcaaa gcctgctgac tgatacccga taaaatcatg tgggccgcaa
     5041 acaactcctg actgactgta tcgtgcaaat cccgagcaat tcgcttccgt tccttctcga
     5101 tgatttcctc ttcctgagca aggctatgat tttcagcttt ttgaagagcc tctgtcaaaa
     5161 ggttaagttt acctgataag gacttgaaac tggcatccaa atcttgatct acaacctgaa
     5221 ctacttcttg ccctgccaat aaacgcttga gattagcctg catttttctt agagaaagct
     5281 cttcgatccc tcgccaaaac agggctaaga gacaggtcat ggacatgctg aaaaccaaca
     5341 ataaaaagac aaatttttct gttttttcga catcgtgcaa aaagatagac cagtcaaaat
     5401 caagtatttc cagcaagctg tgggagaaaa aaaagacaaa taggaaggag gtgagagcaa
     5461 taatgacata ggcttgtttt ttcatcctct aaccacctcc acatcaccaa tcatagtggt
     5521 caagaaaatc ttgacactct tgttactctt gagatagtct tttgtttctt gatgatagtg
     5581 ttcattgcgg agggctcgct tgggctggtt gaaaaaagtc aaatccccat agagacagtt
     5641 aacgctgaga ctgacttcca catctacagg tacgatgatt ttggtcgttc ctaccatctt
     5701 tctgaggata atgacattgt catgattggt taagatgacc ctctccagat gaatagtgtc
     5761 cttacccatg aagcgaaaga gattgatatc atcgaattgg caagtctggt agcttgaaaa
     5821 atgatgaaga tttccaaacc aacgattttt ctccttctta accgtcacga cctcttcaaa
     5881 aaccaaattg gtctgctctt tttcctggtt catcatcggg taaagaagaa agaggctata
     5941 gataaccgca acaaaaatag ctagaatcac aaaaggattg agcataacga tgaaaaagaa
     6001 gagaatggtt gccgctacta aaagaagatt atttccctct ttaccagtgt agtagcgaat
     6061 caaaagcaaa aagaggaata gtatcagcag aaaacgcgaa aaatgctctg ataccatcaa
     6121 aatcagagct cctgtcagaa gacaggcttc gataaataaa aagattttaa attttctcat
     6181 aggttcatcc tctcccttct attttatcac aattcaaaaa agtcacctca gtctgaggat
     6241 ggaaaaaaag gcgctggtta cgcctttttc atctgatcct ttgcttcttt taattttcca
     6301 taaagaagat agtctacttt ttgtagatct gctatggtgg cacagttaag ggaacacata
     6361 atcaagcgta gatctgcttt ccagccttgg acaatgccaa tcacttcttc aactgtgtag
     6421 gtttcaacca attccagaac ggttcgtgac aatcccacag ccttagcacc aaaaaccaag
     6481 cacttaatca tatccagcgg attccgaacc cctccactag ccaagagttc gaccttatct
     6541 ttccattctt gggcattgag aagggcctgc atggtagact gaccccattg attgaggtaa
     6601 tcacgctggc cactacgacg gttttcgata taggcaaagc tggtgccacc acgacccgat
     6661 aggtccactg tacgaacacc gaattcatag gctctttcga ttgtcttggc atccattcca
     6721 aagcccactt ccttgaggac aataggaacg ggaatttgct tgctataatc tgctagatgc
     6781 gattgccagc ttctaaactt cctttctccc tcgggcatga gtaattcctg catgacattg
     6841 acatgcactt gcaatagaac aggattcatc tcttctacag tctgaagtcc taactcgaca
     6901 ggtttgtcca atccaatatt ggttccaagg aggagattgg gatgactaga cttgacagaa
     6961 aaagaatcat ccgttggatt tttgagggct gcgctataag aacccgttac aaataaaata
     7021 ccacaggatt ccgccacctg agccagcttt tgattgattt ctcttccctt attacttcca
     7081 ccagtcatgg cattgatata aaaaggaaag tcccactttc gaccagcaaa ctctgtcgaa
     7141 agatcgattt catccagatt gtaaagaggc aaggaagaat gaatcagctc cacctcatca
     7201 aagctattat aggaactttt ctgctcaagg gcatagagga tatgctcgtc cttacgattt
     7261 gtcgtcatgt cctatccttt cttgatataa gagctcaatc cccagatcgg cccaacgatt
     7321 ttttaaggtt ttggttgatt ccgcatcaaa actcagggcg atgccacagt caccaccacc
     7381 agcaccacta ctcttggcaa cggcctgcaa atcttgactg gcttctttca actgtctaag
     7441 caaaggcgtg taaatatctg tactcaagcc ttctaaaagc ttgctggcta cttctacttg
     7501 ctcgataact ttttcggctt tcccctgttc caaggcttcg accaaagaaa ccaccgtttc
     7561 ttttgaggaa cttaaaaaat tttgattgat attttgcttg atttgctgga ccatgtgact
     7621 cgatacagcc acttccttgg tccatcccac taagaaatca cattctaaag ttggtttcac
     7681 ttgtgaaatt gaaaagcccc aatcacgctc cagaactgtc gccaagtttt cttcttctaa
     7741 ccaagcagcc accttctggc gatcaaatga ctggtagaga accaaatcct ctgccacaat
     7801 acaggcaagg tcgcccatgg aaccattgtc tcctcgctta agcaagacag cgctagtcag
     7861 cttgaacaag agctcctgat caacagaaac atcatacaga gccagtaaag ccttgacaac
     7921 caagacaacg acgctgccac tagaacctag accaaacttt ttcccttctc gttccatttt
     7981 gccacagatt tctagagaaa aaggtcttaa attctgacca cgaacagcga ggaagtctcc
     8041 catcaaagca atcgtttctt gaatcaagct atagtcagga ttaggcctta agtccactgc
     8101 gaaatcaaac atatctgaat agatacggta gctgtcagaa aaagcaatct cagccctcat
     8161 atagatggga atatccttta tcaaagctaa ctgccctggc tctaaaatag catattcacc
     8221 tgcccaatag agttttccgc aagttttaac agcaatcatc ttgactcaaa tcctttgttt
     8281 ttgacacaat caagcgataa cgatgaccga aaatttctga taaatgctcc aagtctttct
     8341 cctgacagaa gaccttaaca ttgggaccag catccatggt aaagtagcag gcctctcctt
     8401 tctcacgaag ctggcgaaca aagtccatag cctcataaga ggcatccgtc agataagaaa
     8461 aggctggact agcagtcttt gtcgtagcat gcatagccag ggcatttttc tccgttaatt
     8521 ctccaatctt ggcaaaatca ttttccttga gataaatcag catatcctga tagtccttct
     8581 cagactgacg aacccagtcg tcgaaagtcg tcgaggtttc cacacaaagt ttcatcccgt
     8641 cacggctaga gattggtttt ttcttgtcct ctagcaccaa cataatcata gctagtttca
     8701 agtctgtctc tacagggtaa atttctccac tatccttatc ccaggctcct agtggtccat
     8761 aaaaactccg agaagaagaa cctgaggcaa atttggcttc ctgtgccaac tgacttctat
     8821 ccaatccaag cttgaaataa gcattacaag ccttgaccag ggcggacaaa ccactagaac
     8881 ttgaggacag acccgctgcc gtaggcatat tgttttgagt atcgatacgg acaaagccct
     8941 caccagctgg acgataacgg tcaataatct tactcatctt ggcatgctcg acctcatttt
     9001 gtagctgacc attgatgtaa aattcgtcag ctgttacatt ggctggtaaa ggcgacaagg
     9061 tcgtctctgt atacatattt tccaaagtta gagaaatact gctagtagca ggcaccatct
     9121 ctttttcttt tttctttccc caatatttga taatagcaat atttgcgtag gaacgtactg
     9181 ttacaggctc tctatccatg tctgaacagc tcctttctct tctaatcttt ctgctagttc
     9241 ttgtgcgtgt gtcaaattgg ttaccaaggc tatgatacaa cctcctagcc caccaccgct
     9301 catcttggca cccagagcac catggctaag agtcgtttca accaaaaagt ctgcctcagg
     9361 gctactgact ccaatttctt ttaaatgtaa atgcgcttga ctgaggattt gtcccagtcc
     9421 ttcagcatct ttttgtgaaa tcgcaacttc tgcttgctgg gttaattctc ccaaggcatg
     9481 caaaaacggt agggcatcct tgcccttatt ttgaaccact tggatggctt cacgagtatg
     9541 accataaaca cccgtatcgg caatcaccaa ataggcggat aaatccatct caagttctgt
     9601 aaatcctacg ttcttgataa agcgaatagg ttggtcacta agacaggtct tagcatccaa
     9661 accactagga ttcatatggg caatcatttc agctcgattg accaagattt ctagtacatc
     9721 atgaggcaga tcagcctgat agtagtcaaa tactgcacga atggccgcta tgctgatagc
     9781 cgctgacgaa cccatccccc gtttctcagg gatagccgag tcaatctcac aacgaatgca
     9841 ggcttctgtg atattcaaat actccagtga ggcataaacc gccatggaca aggtatcctc
     9901 ctcataaagg cgccaaggac tctctgcagg aactacctta caggtcacct ccacctccaa
     9961 aagaggcagg gaaatggcag gataaccgta aacgaccgca tgttccccta ttaaaattat
    10021 cttactatgt gcctgaccga caccaacttt ttttgtcatt ttttcctttt actagacgaa
    10081 aaaacgtctt atttttcata caagtattaa ttctttccta tctattttat tatattttca
    10141 caaaaaaagc gattgtttcc attcacaatc gcttctttca ttattgaacc cattcgccat
    10201 tatagttgac agaatagcca tctacggtcg tattcactgc caaggcacct gagcgctata
    10261 agcgtagtac catctgccat tgacctggaa ccaacctgtc gtcatagaac gacgaaagaa
    10321 actccatacc attaagtaaa gaggaaagtc gtgagggagc atgcgccatt gacaacctgt
    10381 tttagtgacg tacaaagtct cattaacaag tactcgtttc ggccatttat aggtgcggtg
    10441 tttggagaaa tagggttcaa tcttcgccca ttcttgatcg tttaaatcag tatcatatgc
    10501 tttgcgtatc ataactctag cttaacattt ttttgtgaat acaggttcta aataatcgac
    10561 cacgaaaatt tcttaagtgg aaaacgcctt atgaagtatg ctacgggaaa cttatgcact
    10621 taatttgaca attcaagatg taaaaatata t
//
LOCUS       DAVKQB010000063        10567 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKQB010000063 DAVKQB010000000
VERSION     DAVKQB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.063"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            31..348
                     /locus_tag="VW528_001837"
     CDS             31..348
                     /locus_tag="VW528_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU9912612.1"
                     /translation="MVPQFSRGLPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDA
                     ELEALEEEKKKNMEDMPMFNKDNTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGR
                     PVR"
     gene            257..1288
                     /locus_tag="VW528_001838"
                     /pseudo
     CDS             257..1288
                     /locus_tag="VW528_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795723.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            1648..1875
                     /locus_tag="VW528_001839"
     CDS             1648..1875
                     /locus_tag="VW528_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HEU9912613.1"
                     /translation="MAILDDLQALYDNGWDASFNYNGQVCGIFPNSIYDVVVIIADDE
                     YRASSFDDLISLQIEGKTLPEIMNEVEVQYG"
     gene            2024..2236
                     /locus_tag="VW528_001840"
     CDS             2024..2236
                     /locus_tag="VW528_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912614.1"
                     /translation="MEEWKERFKKEYYELKERFQKLDMMIGKYEKGQLEFESKCPIDS
                     LKGQRSTMWNYLRILEQRAKIEEIKL"
     gene            2406..2990
                     /locus_tag="VW528_001841"
     CDS             2406..2990
                     /locus_tag="VW528_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEU9912615.1"
                     /translation="MSEEINATVSTESTETVDTQGNVDSVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTSAETVSQSVAVLQKSWEQAVQKGVQEKLKGGAPMKQAPV
                     DSDGITKEEFARMGYQSRNELYQKNPELYKKLKG"
     gene            2997..3842
                     /locus_tag="VW528_001842"
     CDS             2997..3842
                     /locus_tag="VW528_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HEU9912616.1"
                     /translation="MTAGQTKLATMVNPEVMADMVAAKLPKLIKFTPLAYVETKLEGQ
                     PGSTLTVPAWEYAGDATEIEEGQAITPDQLTTKKTTMTIKKAGKGYEITDESLLSGLG
                     DPLGQATYQLGLAIANKIDNDLVAVAKTATQHITETPTTLEAIDKALDIFEDEEDAQY
                     VAIINPKDATKLKTAVAKEWIKGSELGANMVVSGTFGEVDGVQIVRSKKVDEGKGFLV
                     KVSPSQTQTDDANKYGAFVILLKRDVAIETDRDILKKTTVITGDEHYGVYLYDPTRVV
                     KFGGA"
     gene            3854..4036
                     /locus_tag="VW528_001843"
     CDS             3854..4036
                     /locus_tag="VW528_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEU9912617.1"
                     /translation="MSLLLRRHYIQEEQVNQYSDLENKTLEELKDLAKEAGVAGAYKL
                     TKAEIVEVLEELKSEI"
     gene            4026..4214
                     /locus_tag="VW528_001844"
     CDS             4026..4214
                     /locus_tag="VW528_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912618.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNIASNGVT
                     ISDVLEKILPEPEFLEED"
     gene            4216..4527
                     /locus_tag="VW528_001845"
     CDS             4216..4527
                     /locus_tag="VW528_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEU9912619.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGEHSRTEGGIAVVYGENGLSTDLLQRIRMHRLARVAGHVFEA
                     E"
     gene            4508..4846
                     /locus_tag="VW528_001846"
     CDS             4508..4846
                     /locus_tag="VW528_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912620.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            4821..5336
                     /locus_tag="VW528_001847"
     CDS             4821..5336
                     /locus_tag="VW528_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU9912621.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELITSIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPIGQASHSGISPEV
                     SVTYKSSPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            5333..5701
                     /locus_tag="VW528_001848"
     CDS             5333..5701
                     /locus_tag="VW528_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912622.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGIVDLHSELVFQFRMEN"
     gene            5704..6117
                     /locus_tag="VW528_001849"
     CDS             5704..6117
                     /locus_tag="VW528_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9912623.1"
                     /translation="MLANGITLSYGETKDSYTKLVGLKEVPEFGIELEKVENTTLEDT
                     VKKYEFGIGDIGELEYKFSYNNSSATAPYRVLRKAADDKKKLYFEQAYPDGTKVIFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSNLTFTDGIGG"
     gene            6122..6502
                     /locus_tag="VW528_001850"
     CDS             6122..6502
                     /locus_tag="VW528_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912624.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSLEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVDK"
     gene            6541..6891
                     /locus_tag="VW528_001851"
     CDS             6541..6891
                     /locus_tag="VW528_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912625.1"
                     /translation="MFLDIEGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKAFEFWEYAPELFVEEQQAVEQERQKQALLLHKERMREFAE
                     RHNRKRKEEVNGNS"
     gene            6878..10540
                     /locus_tag="VW528_001852"
     CDS             6878..10540
                     /locus_tag="VW528_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912626.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSSSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGDTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGGK
                     GKGGGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIER
                     IKTALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESI
                     ANGLGRQKERITRALVALFDNIGNISEAVGNIAQDFSSTFYDVITSTGAVRIGSAIVS
                     TLLSLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFESIERSVN
                     KFGDGLSRVYDEHVAPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGI
                     SDLLGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPILALITTWQTINFLSWAEQ
                     AGGLAGAFSLLGSKVSLIVGGIKNLGLAIKALTFDKLVSFGETIYLNTLYAKDFVVNS
                     GKTIAQLGKTALELGKSALAWTAHAAKMGLATAAEFAHSVAAGVATAATWAFNAALAV
                     LTSPITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICRAIGEFFSGL
                     WTKLQEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSW
                     FGQRWADVTSALANIGAWFTDIFQKAWTGLTNIFSKLGLWFGERWADVTSVLANVSSW
                     FGNMFTSAYNAVKNAFSSIGGFFSGVWSTVQSIFVNAGQKVGSAVGGAFKSAVNAVLG
                     TIENVVNDFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAMIGEAG
                     KEAVVPLENTGFIQTLGRVVSSAVVNAMAGISPQGGFSSDGDIVIQIAGHEFGRVAIQ
                     EINKEHERAGQTLLKI"
BASE COUNT         3334 a         1870 c         2563 g         2800 t
ORIGIN      
        1 atgaagcaga ttgctttaga gtctcattca atcgttccac aattcagtcg tggtttgcct
       61 aagaacttat tagaaatctc tcagattgtg aacaatttgg aaggcaaagt gaccaataga
      121 cagcttattt ctctcttgcc gtttgtggaa gaccctgacg ctgagctgga agccttggaa
      181 gaagagaaaa agaagaacat ggaagacatg ccgatgttca acaaagacaa cacgaaaccc
      241 gaagacgagg tagaggatga agaatcagga gtattgggcg aagaggaaag ccaatctgat
      301 ttaccagcag atggacaagg ccgaaaagca ggcagaccag ttcgataagg tctatcagga
      361 agctaagact tacttggata aggaaatcaa taagattttc gataaattcc aacgtgatta
      421 tggtctaagt caggtagaag ctagacaagt cttgaagaac atgaaagaca agaaaaatct
      481 gaatgaactt cgtaaagtac ttgaagcgag accgaatgac ccgaacatcc aaagattact
      541 agctgactta gacagcccag cttattcttt ccgcatgaag cgcctagagc gtttgagcga
      601 cgatttagat cgtatgcgtg aatctatcta tcattcggag aagacaggct cagatgcctt
      661 ttatagcgac ctgatgaagg atagttacta caaggctacc tttgacctac aacagcagac
      721 aggactagca tatggctttt ctgggcttcc tgagaacgag attaaacatc tacagtcttt
      781 tagttgggta ggagatggaa gtacgtactc aacagacatc tggaagaata caggaaaact
      841 tacttccagc ataaaagatg aactcctcat tagcctcatg acaggcagag atacacgaga
      901 aactgcacaa gcaattgctg agaggttcaa tgtaggtcag aacgatgcaa ggcgtttggt
      961 tcgaacagaa tccgcctttt ttcataacca gatggagcta ctcagctatg aagaagcaga
     1021 catagaaaag tatatctttg tggccgtctt agacaagcgt acatcacgca tttgtcagga
     1081 gcatgacaat caggtctatg atagggataa ggctgtccct ggtgtcaatt gtccgcctat
     1141 gcacccttgg tgtaggtcta ctactgtcgg atacgatgag gacgcagact acagcaagtt
     1201 gaagcgcaga gcaaggaatc cagagacagg taaagttgag tacgtgcctg ccgatatgac
     1261 ttataaagag tggtatagca agtatgtgga tggtaataga gagtctatta aacgtaaagc
     1321 gtttgataaa actattaaag atggtataat agtaagtgta tcagggacta caattggaca
     1381 cactccgcct ggcaaaatag gtttgcctaa tagtgtagtt cagcataatg ctacaaacgg
     1441 agatgtcctt ggtagaactt actatgatgc tagaggtttt aaaacgaaag atgttcattt
     1501 tacaaaccat aaacaaccgg cacgtcatcc ttatggaaaa atcggagaac atgctcatga
     1561 ttttgtattt gatgatgaag gtaagttcgt tagtaggagt actagggaat taacagacga
     1621 tgaaagaaag gagaatcaag atatattatg gcgatattag atgatttaca agcgttatat
     1681 gataatggat gggacgcttc ttttaattat aatggtcaag tatgtggcat tttccctaat
     1741 tctatttatg atgttgttgt tattattgcg gacgacgaat atagagcatc ttcttttgac
     1801 gatttgattt ctttacagat tgaagggaaa actttaccgg aaatcatgaa cgaagttgaa
     1861 gtacaatatg gctaaagcac ctagagagat ctaagtgctt ttttagtacc cagaaaggag
     1921 actatccgta cgattttctt gattgtgaat ttgaagtaaa aagaatttca gtgatagctt
     1981 gcgatactat ccttatagaa gcctaaaaga aaggaacaga aaaatggaag aatggaaaga
     2041 aagatttaaa aaagaatact acgaattgaa agaacgattc cagaagttag atatgatgat
     2101 tgggaaatac gaaaaagggc aactagagtt tgaatctaaa tgtccgattg attcgttaaa
     2161 aggtcagcgt tcaaccatgt ggaattattt aagaattcta gaacaacgtg caaaaattga
     2221 agaaattaaa ctataaaaat taaccgcatc gaaatcgatg cggttttcct atgccctaac
     2281 cgtatggaat cccgtacggt ttttatattg tccaaactgt accgatgaca ataaaagctg
     2341 tgctgttccg tcgccggacg taaaacgaga ttatcgagtg gcgacgtaat cgctggagga
     2401 caattatgtc agaagaaatc aatgcaactg tatctactga atcaactgag actgtcgaca
     2461 ctcaaggaaa tgttgattca gtgcaggaag aaaagcacga acgaactttc actcgtgctg
     2521 aaatcggtaa gatgctatct gccgaacgct ctaaatggga agctgagcaa gaagccaagg
     2581 aaaacgaagc taagaaactt gccaagatga acgctgacga gaaacaaaaa tatcagttgg
     2641 atcagcgtga gcaagaacta gctgaccgtg agaaggctat tgctcgcaag gaattgaccg
     2701 cagaagctaa agcaatgcta agtgaacgtg acttacctgt tgagttagta aatgtagttg
     2761 atttgacaag cgcagagacg gtatcgcagt ctgtcgctgt attgcagaaa tcatgggagc
     2821 aagccgtgca aaaaggcgtt caagaaaaac taaaaggcgg agctccaatg aaacaagcgc
     2881 cagtcgatag tgacggtatc acaaaagaag aatttgctcg tatgggttat cagagtcgaa
     2941 atgagctcta tcaaaagaac ccagagcttt ataagaaatt gaaaggataa tagaaaatga
     3001 cagcaggaca aactaaatta gccactatgg ttaatccaga agtaatggcg gatatggtag
     3061 ccgctaaatt acctaaattg attaaattta caccgctagc gtatgtagag acaaagcttg
     3121 aaggtcaacc aggtagcact ttaacagtgc cagcatggga gtatgcagga gacgctactg
     3181 aaattgaaga aggccaagca attacgccag accaattgac tactaaaaag actactatga
     3241 ccatcaaaaa agcaggtaaa ggttatgaaa ttaccgatga gtctcttttg tcaggtcttg
     3301 gtgacccact aggtcaagcg acttaccagc ttggtttagc tattgccaac aagatcgata
     3361 atgatttggt agcggtagct aaaactgcaa cacaacatat tacagaaact ccaacaactc
     3421 ttgaggcaat cgataaagct ctagatatct ttgaggacga agaagatgca cagtatgttg
     3481 ctatcatcaa ccctaaagat gctactaagc taaaaactgc agtagcaaaa gaatggatta
     3541 aaggttcaga gcttggagca aatatggttg tttctggaac cttcggtgaa gttgatggtg
     3601 tgcaaatcgt gcgctctaaa aaagttgatg aaggtaaagg cttccttgtt aaagtgtcac
     3661 caagtcaaac tcagacagac gacgctaaca aatatggagc ttttgttatc ttgcttaaac
     3721 gtgatgtggc tatcgaaaca gaccgcgata tcttgaagaa gactaccgta atcacaggtg
     3781 atgaacacta cggcgtttac ctttacgacc ctacacgagt tgtaaaattc ggtggcgcgt
     3841 aagaggtgac gatatgagtt tattgctacg acgtcattat attcaagaag agcaggttaa
     3901 ccagtattct gatttagaga ataaaactct agaagagttg aaggatctag cgaaagaagc
     3961 aggtgtagca ggcgcttata agttgacaaa agccgaaatt gtagaagttt tggaggaact
     4021 aaaaagtgaa atttaaaatc aaacaagatt tctatgattg ggaatcaaat gtgaaacgac
     4081 tggcaggaga ggaacttgag attactgagg agcgctatgc tgagctggct gacaatattg
     4141 ccagcaacgg tgtcactatc tcagacgttc ttgagaaaat cctccctgaa cctgagttct
     4201 tagaagagga ttgatatgtc tatagagttg ctgaagaaat taacaggcga agaagatact
     4261 cagcttctca tgttgctcca aacgagggct acaaatctta tcttgtcaga gactaatcgc
     4321 acatctttga cacctgcttt aagtctttta atacctgagg ttgctatcga actccacaac
     4381 cgctcaggag cggaaggaga gcattctaga accgagggtg gtatagcagt agtctacgga
     4441 gaaaacggcc tgtctacgga tcttctacag cgaatacgca tgcacaggct agcaagggtg
     4501 gcaggtcatg tttttgaagc agagtagact gaaaccttat ccaatgcgac ggtttgaaaa
     4561 gactgtcact gaggaaggtg tcgcgaaaga agggtatgcc aaggaagctg agacagtccg
     4621 ccttgaattg tggccagcta gtagcaagct acaatctgaa ttgtatggcg agcgtgtcaa
     4681 tgatattttg aacgcaaatg ccaacaagtc agctactatc aaagtaaaag atggtgtgtg
     4741 tatcgatagt cagacggaag tgactcatag ggttatttct aaaaaggtct acacacatca
     4801 tcaagttttg gagttagagc gtgtcagggc tactaggggc agataggctt atagctaaat
     4861 gtagacgatt ggctagtaaa aaaactggcg aggatatcgt cttacgtgcg gtgcacaatg
     4921 ctactataaa ggttgtccaa gcagatgcaa gaagactcgc accagcgaga gatggagagc
     4981 ttataactag tatcaaaact agggcaaaaa tggacggaga taaggctata ggcgaagttt
     5041 acaccaacct taaatacgct ccttatgttg agtttggaac gggaccaata ggacaagcta
     5101 gccattctgg tatttctcca gaggtcagcg tgacttacaa gtctagtccg tggtatgtac
     5161 atgaagacca aatcaatgta ggaccgtacc actttcaaaa aatcggggag ttctacaaga
     5221 tgtatggtca acctgcccag ccttatcttt atccagcttt gagagacaat caagagcgtg
     5281 tgtctaagaa tatttcgaat tatgtccgta gaaagataag agaacaaata aaatgattaa
     5341 tatcaagcct gttatttata aagaattgca aaaggttgca gataatgtga ccgatactta
     5401 tcctagcgat tgggagactt tcccagtcgt tatttttttg gaagaacaaa acaagccggg
     5461 tgattggttt gatgaccagg aacaaaaatc ctctatccgc tacaaggtgg atatctttga
     5521 tgatactagc actagtgagt tagctgttaa aatcaatcag atttttgagt ctttaggttt
     5581 gcgaagaact gactgccaag acgtgccaga cccgtctcat ttgagacata aggtcatgcg
     5641 ttttgaaggt atcgtcgact tacactcaga gcttgttttt caatttagaa tggagaatta
     5701 aacatgttag caaatggaat tacgctttct tatggcgaaa caaaagatag ctatactaaa
     5761 cttgttggat tgaaagaagt accagagttt ggtattgaac tcgaaaaagt agaaaatact
     5821 actcttgaag atacggtgaa gaagtacgag tttggtattg gggacatagg agaacttgag
     5881 tacaagttct cttataataa ttcaagcgca actgctcctt atcgtgtatt gcgtaaggca
     5941 gcagacgaca agaagaaact ctactttgaa caagcttatc cagacggtac taaggtcatt
     6001 tttgaaggcc aagtatctgt taagcttggc ggtggcggtg tcaatgccgt tatcgatttc
     6061 acacttaaga ttgccttgca gtctaatctt acatttactg atggtattgg aggttaatta
     6121 aatggcgtta aaatacacaa cttggaaagt tactgacgaa aaagagttga agctacgttt
     6181 gacatctcat caagctgcaa ctgtggaaga aaaaatcggc atgaacttgc tgaagatttt
     6241 catgcctgaa gctggcgaag agttcacttt accgcctttg aaagttatgt tgttgttagt
     6301 tcacggagcc ttgcagcagt atgaacatgg gtattctctt gaggatgtct atgatttata
     6361 cgatgaatac gtggacaatg gcggagacca aacaaccttc atgacagagg tgttgatgcc
     6421 actctttgaa gtatcgggtt ttactccacg aggaagcaag gacaagaaaa cttccaagaa
     6481 gaaaatgaca gtagacaagt aatcttaacg gtaactcaga ttattgagag gctttatcct
     6541 atgtttttgg acatcgaggg caagcctctt gatttttggg atttgacggt acttgaaatc
     6601 agagaaatga ttgaaagcta taaccgtgtc aaaatccaag agcgtaaaga aaagattatt
     6661 gactcataca gactttcgca gatgatatcc aaccacgttt ctttattgtt atccaaagat
     6721 gccaaggcct ttgagttctg ggaatatgcg cctgagttgt ttgtagaaga acaacaagca
     6781 gtagaacagg aacgacagaa acaagcactt ttgttgcata aggaacggat gcgtgaattt
     6841 gcagagagac ataatcgaaa aaggaaggag gaagtaaatg gcaactcttg atgaattgaa
     6901 agtcatgatt gacgctgaga tagcgccttt caggaagaag atgaaagaag tcgagaatca
     6961 ggtcaaagga acatctgacc aagtgaaaaa tgctactgcc aaagttcgtg aacagtcgag
     7021 ctcaatcggt agtgcgtttg gcaagctggc taagttcgct ggttttgcaa tccttggtaa
     7081 gaaattactt gatgttggga tgtattcaac gcagacggct cttgaagtat cagcgtctat
     7141 gaaccaaatc aagcgacaga tgggcgagag ttcgcaatct ttcttaaaat gggttaacga
     7201 taatgccaac gctatgaata tgggtgtggg tgaggctacc aactacggtg cagtctactc
     7261 aaacttattt tctggattta tcaaagatac caacaagcta agcgcctata ccgctaagat
     7321 gttgcagaca tcggcagttg ttgctgaagg ttcagggcgc acgattacag acgttatgga
     7381 gcggattcgc tcaggtttac tagggaacac cgaagcgatt gaggacctag gaatcaacgt
     7441 caacgtggct atgattgagt ccactgaagc ctttaagaag ttcgcaaacg gacagagctg
     7501 gcaacagttg gattaccaaa cccagcaaca aatccgcctt atggctattc tggaacaggc
     7561 tacagccaag tatggggata ccttgtctaa ttctgtaaat ggtcgtatca gcctatttaa
     7621 gtcgctgatg aaggacgcag cattgaacct tggtaactct atgttaccga ttatcaatgc
     7681 cattatgcct gtcttgaact cttttgctat ggtcttaaag aacgttactg ctaaactcgc
     7741 tgagtttatc gctttgatgt tcaacaagaa agcaacagtg aaagatggtg ttggtggagc
     7801 agttggagac atgggtaacg ccatgaagga tgctgcaggc ggagcaggag accttgctga
     7861 cgcagtagac gacgctggag attcagcagg aggacttgct gacaatcttg gagactccgc
     7921 caaaaacgct aagaaggccg ctaaagagtt gctaggtctt ttgggatttg atgagattaa
     7981 catcttgcaa aaaccaaaag atgacgacgc aggcgggtct ggaggcggtg gcaaaggtgg
     8041 taaaggtggt aaaggaaagg gaggcggtgg cggacctttc aaagacatct tgccagaagt
     8101 cgagttgacc gacatggaca acaaattcaa gagcattttt gatggtcttg gagataagct
     8161 caaagggttg tttgacctct tcaagaaagg ttttgatgca gcatttagac cagaaggtat
     8221 agaacgcatt aagactgcct tagaccaaat agctaagaca atgggagaaa tcgccactga
     8281 cccaagggtt gtgaatgcct ttaaccgaat ggctgagaaa attgcttatg ctttagggca
     8341 agtgacaggc tcaataacaa ctatcgggct aggtatcggt gttttccttg ccgaaagtat
     8401 tgcaaatggc cttggaaggc aaaaagaacg cattaccagg gcgctagtcg ctttgtttga
     8461 taatattggt aacatttccg aggcagtagg aaacatcgct caggactttt ctagtacttt
     8521 ctacgacgtc attacctcaa ctggtgcggt tcgtatcggt agcgctattg tgtcaactct
     8581 gttgagtttg acatctacca ttgttgaagt tggtagtaaa ttagcaggaa gtttgtttaa
     8641 aggttttgaa aaagtcgttg tgacaagcgc tcctaaaatt tcatcagtct tccaaagttt
     8701 attagatact gttgcgcctg tatttgagag cattgaaagg tctgttaaca aatttggcga
     8761 tggcttaagt cgtgtttatg atgaacatgt agcccctgct attaactcta ttgctaatgc
     8821 ttttaacgga ctgattgata tcatacaaat cctctgggag aattcttggc aaccttttgc
     8881 tgagttttta tcaggagtat tcggtgttag tattgaagga atttcagatt tattaggagg
     8941 tggcctttta gccactttgg gactattggc ggatgctatt aagttagtgg cagatggttt
     9001 caccgttttt tctgactggt gtaaagaaaa caaagaacct atcttggctt tgataacaac
     9061 ttggcaaacg attaatttct tatcatgggc tgaacaagcc gggggacttg caggagcatt
     9121 cagcttgtta ggtagtaagg tctctttgat tgttggaggg attaagaatc taggtcttgc
     9181 tattaaagca ttgacatttg ataagttggt cagttttggt gaaacaatct atttgaacac
     9241 cttatatgca aaagattttg tggtcaattc aggtaaaaca attgcacagc taggaaaaac
     9301 tgctttagaa cttggtaaat cagctctagc atggactgct catgcagcga aaatgggatt
     9361 agcaaccgcg gcggaatttg cacattctgt tgcagcagga gtcgctacag ctgcaacatg
     9421 ggcttttaat gcagcgttag cagttttgac aagtccaata acatggatta ttgcagcaat
     9481 cgcagctttg attgctatcg gtgtcttgct ctaccaaaac tgggacactg ttgttgagtt
     9541 tgctaaaact gcatggcaag gactatgtga ttttattagt ggtatttgtc gagctattgg
     9601 cgaatttttc agtggtctat ggacaaaact acaagaaatc tttgagccga taggtcaatg
     9661 gtttggcgag aagttccagc aagcatggga tgccattgtc aacatcttct ctggtatcgg
     9721 agagtggttc tctggtgtat tccaaggtgc atgggacgct atcgttaata tcttcactcc
     9781 aatcggctca tggtttggac aacgttgggc agatgtgact agtgctttgg ctaatatcgg
     9841 ggcatggttt actgacatat tccaaaaagc atggactggt ctaacaaaca tctttagcaa
     9901 actaggttta tggtttggcg agagatgggc agatgttaca agtgttcttg cgaatgtatc
     9961 ttcttggttt gggaatatgt ttactagtgc ttataatgca gtcaagaacg cgtttagttc
    10021 aattggtggc ttcttcagcg gtgtatggtc aacggttcaa agcatatttg tcaatgctgg
    10081 acaaaaggtt ggtagcgctg taggtggagc gtttaagagt gcggtcaatg cggttcttgg
    10141 aacgattgaa aatgtagtca atgacttcat cggaatgatt aatggagttt taggcgttgt
    10201 cagaaactta cctggtctag gatgggttgg tagtgtaagc acagttagcc tccctcgtct
    10261 tgcccgtggt ggtatcgtcg atagtccaac aatcgccatg attggtgaag ctggtaaaga
    10321 ggcggtcgta ccacttgaaa atacaggatt tatccaaaca cttggacgag tagtcagcag
    10381 tgcggtagta aatgccatgg ctggtattag tccacaaggt ggtttttcta gcgacggcga
    10441 catcgttatt caaatcgcag gccatgagtt cggacgggta gctatccaag aaatcaacaa
    10501 ggaacatgaa cgagcaggtc aaaccttgct caagatttag gaggttaaat ggcacaattg
    10561 acaatca
//
LOCUS       DAVKQB010000064        10080 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKQB010000064 DAVKQB010000000
VERSION     DAVKQB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.064"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..237)
                     /locus_tag="VW528_001853"
     CDS             complement(1..237)
                     /locus_tag="VW528_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9912627.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(305..1225)
                     /locus_tag="VW528_001854"
     CDS             complement(305..1225)
                     /locus_tag="VW528_001854"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9912628.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            1423..1827
                     /locus_tag="VW528_001855"
     CDS             1423..1827
                     /locus_tag="VW528_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912629.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            2043..2624
                     /locus_tag="VW528_001856"
     CDS             2043..2624
                     /locus_tag="VW528_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9912630.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETSGDLTIVIQKKQ
                     GAPSAKSKMEGVFGNCEILKKDKGYYILRSVKE"
     gene            2621..3898
                     /locus_tag="VW528_001857"
     CDS             2621..3898
                     /locus_tag="VW528_001857"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9912631.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILVPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            3916..4578
                     /gene="deoC"
                     /locus_tag="VW528_001858"
     CDS             3916..4578
                     /gene="deoC"
                     /locus_tag="VW528_001858"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9912632.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            4565..4954
                     /locus_tag="VW528_001859"
     CDS             4565..4954
                     /locus_tag="VW528_001859"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9912633.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            5044..6096
                     /locus_tag="VW528_001860"
     CDS             5044..6096
                     /locus_tag="VW528_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9912634.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            6240..7775
                     /locus_tag="VW528_001861"
     CDS             6240..7775
                     /locus_tag="VW528_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912635.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            7768..8826
                     /locus_tag="VW528_001862"
     CDS             7768..8826
                     /locus_tag="VW528_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9912636.1"
                     /translation="MFKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            8829..9785
                     /locus_tag="VW528_001863"
     CDS             8829..9785
                     /locus_tag="VW528_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9912637.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            9870..>10080
                     /locus_tag="VW528_001864"
     CDS             9870..>10080
                     /locus_tag="VW528_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9912638.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSNIRYMIKLIDR
                     YGIEFVKKGKNRYYSPDLKQEMIHKV"
BASE COUNT         2892 a         1767 c         2318 g         3103 t
ORIGIN      
        1 ttatttagca agtttagctg aaagacgagc tttatcgcgg cttgctttgt ttttgtgaat
       61 caaaccttta gtttctgctt tatcgatagc tgagctagca gcacggaaaa gttcttcaga
      121 tgggtttgct tcgaaagctt ttatagcagt acgcatagct gatttttgag ctgagttctt
      181 ttcgttttgt ttaacgttca attcagcgcg tttgatagct gatttaatgt ttgccaatgt
      241 tcttacctcc atatttacta actataccat tatatatgaa aacttacgtt ttgacaaggg
      301 aaaattattt ttttaagtaa atttcatcga tttcatggtt cttgctttta tgaagaatca
      361 cttctgcacg atttctggtt ggttcaatat aattttgtag atttgtgaga ttgatactgg
      421 tccagacctg atgggcaaag gcttccactt ccccaatcgg catctgagta aaacgataat
      481 agtagctatc agggtcgttt tgggctagac tcagcatttt caagaaacgg tccagatacc
      541 aactttcaat atcatccact ccagcatcta catagatgga aaagtcaaag aagtcagtga
      601 tatagagacg atcgttttgt ggattttgaa agacattgat tccctcaaca attacaaaat
      661 cagcagcttt gacactttgt ttctcttcgg gtacgatgtc gtaaacttca tgagaataga
      721 caggaatatc tacatcttgt ccatttttga tgcggtccaa gaagttgaga agagcttcca
      781 tatcatagct ttcaggaaat cctttacgat ttaaaatccc ctgctcaatc aaggtttgat
      841 tgggatagag aaaaccatca gttgtaacca actcaaccgt agcatctgta aacgtacggg
      901 acagtaggat ttgaagtagg cgactggttg tggattttcc aacggcaaca ctcccagaaa
      961 ccccaataat aaaaggttga gatttacttt cacgttggag gaaaattcct tttgaaaagg
     1021 ctaaatcttc cttagttcgc ttgtaaatct gaatcagatg agccaagggg agatagatat
     1081 ctgtaacgtc ttggagactg atttggtcat taaaactctt gatagattcc aattcttctt
     1141 ctgtcaaagg aggtgttgtc tttcgatgta aagattgcca agtctggcgg ctgatttttt
     1201 caaaatgtaa aaattcgttg gtcatttgtt ttcccctaga tattttgtct atcataccat
     1261 aaaaatctaa aaaaataaag cggtttcttg aatgtggtaa gtcaatggta tatagtataa
     1321 gtgatcaatt tattgatgaa ataggagata gattctactg atttataagt ttataagctt
     1381 ataaaggggg ctttttagtt cacaacttat aggaggtgta ctatgaaaat cttaaaacgt
     1441 tacatattgg aactctgttt tattttaagt tttgctttac cttttataaa aggagccaat
     1501 gcagataatg gtagatgctt tgtggaaacc tattacggtt ttactttttt gatggaacat
     1561 gctattgtaa cagctgtctt tatctattcg tttttaattg ctttcttact aaaaaaacga
     1621 tgggcgaaat ggattgctgc tggtagttat tgctttttag tcctatggat tgcgacagag
     1681 ggttatttct tccgcatgtc actagaagat ttgatacgac tttggacaag tttggaaatc
     1741 ctgacacaaa cgtatcagtt gggcttttat ctaaacattt tgttgggaat tttgttgata
     1801 ataaagtatt ttaaggttaa gcaatagaaa taataatctg cctgattata gatgataaac
     1861 tttttgtttt tatctggact gtgatattta tagttctaaa tggtttatta tttataagtg
     1921 ttgaaagaga ttgaagaaaa tggcttcgtg actcgctttc ttcggtcttt ttcgttttat
     1981 tatcaatttg taaacgattt acaatttgag tcaaattatg gtaaaatagt gtcatgagta
     2041 aaatgtatta tgcagaaaat cctgacgctg ctcacgacat tcatgagttg agagtagagt
     2101 tgttgggaca aaaaatgacc tttttaacgg atgcgggtgt ttttagcaag aaaatagttg
     2161 actttggaag tcaactattg ctcaagtgtc ttgaggtcaa ccaaggagaa actgtccttg
     2221 atgtaggctg tggttacgga cctttgggtt tgtccttagc taaggcttat ggagttcagg
     2281 ctactatggt tgatatcaac actcgtgcct tagatttagc tcggagaaat gctgaaaaaa
     2341 ataatgcaaa agcgacgatt ttccagtcca acatctatga acaagttgaa ggacattttg
     2401 atcatgtcat ttccaatcct cctatccgtg cgggcaagca agtcgttcat gaaatcattg
     2461 agaaaagtaa agatttcttg gaaactagtg gggatttgac aatcgtcatc cagaaaaaac
     2521 aaggggctcc aagtgctaag tccaagatgg agggtgtgtt tggtaattgt gaaatcctta
     2581 aaaaagacaa gggatactat atccttagaa gtgtgaaaga atgagagcag ttgatttaat
     2641 acaaaaaaaa cgagacggtc aagaactgac ttcaagtgaa attgaatggc tggtagaagg
     2701 ctatgtgtca ggaactgttc ctgattatca gatgtctgcc tttgctatgg ctgtttattt
     2761 taaaggaatg acgactcgag aaatctctga tttaacgatg aatatggtta agactgggca
     2821 agagtttgac ctgtcagcca ttgatggtgt gaaagttgac aagcattcta ctggtggtgt
     2881 cggcgataag gtaaccttga ttttagttcc tcttgttgct agcttcggtg tgcctgtagc
     2941 aaaaatgagt ggtcgtggtc ttggccatac aggcggaaca attgataaat tggagtccat
     3001 taagggctat caagtagaac gtagtcaaga ggattttatt cgtcaggttc aggatattgg
     3061 agtatctgtc attgggcaat cagaccagct ggtcaaagca gataagcttc tctatgccct
     3121 tcgtgatgtg accgcaactg tcgacacgat tcctttgatt gcgagttcgg tgatgagcaa
     3181 gaaaattgcg gcaggggcgg atgctatttt gctagacgtg actgtcggtg agggtgcctt
     3241 catgaagacg gttgatgagg cgcgtgagtt ggctcaaact atggtagatc ttggtaaggt
     3301 tgttggacgg aagacagtag cagtgattac ggatatgagc cagcctttgg gacgagcgat
     3361 tggaaatcgt ctggaaatcc ttgaagcatt ggagattttg caaggtcaag gacgtcagga
     3421 tattacccac tttatttgtg aattggctca aattatgctt ggtttggcaa atgttaacaa
     3481 aactgttgaa gaagttcgcc aacatcttga gaatggtcaa gcactggcta agtttgagga
     3541 aatggtgcaa gcccaaggtg gagacttgga agacctctat cgtcctgtaa atgtagctca
     3601 tgtggtggaa atccctgctc aggaaacggg tgtcatttca gctcttccag ctatggactt
     3661 tggtctttat gccatgaggc tgggtgctgg tcgtgcagtt aagtctgatg ccttggacta
     3721 tgaaacagga attgtttttg aaaagaagat tggagactcc gttcaaaagg gtgaaattgt
     3781 tgcaaaagta tacacaaatg aaaaaattcc acctcaacta gttacagatt tccaaaaatg
     3841 tgttaaaata agtgatgaag tgaaaaaaat ccgtgaaatt gtagaaatca tctcctaaat
     3901 ggagggaaaa ccgaaatgaa attaaataaa tatatagatc atacgctttt aaaacaagat
     3961 gcaaagaaaa aacaaattga tagtttgttg tctgaggcta gagagtatga ctttgccagt
     4021 gtttgcgtta atccgacctg ggttgaacat gctaaaaaag gacttgaagg cacagatgtt
     4081 aaggtttgca cagtagtagg tttccctttg ggagcaacaa cttcagccgt gaaagcattt
     4141 gagacaaaag aagctatcca aaatggtgca gatgagattg atatggtgat caatgttgga
     4201 gctctcaaat caggtaattt agccttggtt gagtcagata ttcgcgcagt agtggaagca
     4261 agtggtgata agttagtgaa agtcattatt gaagcttgcc ttctgacaga ccaagaaaaa
     4321 attgttgttt gccaattggc ccaaaaagct ggggctgact ttgttaaaac atctactggt
     4381 ttttcaactg gtggtgctac gatagcagat gttacattaa tgcgtgaaac agttggatct
     4441 gatatgggag tcaaggctgc tggtggagct cgttcttatg cagatgctct tgcctttgtc
     4501 gaagcaggtg cgacccgtat cggaacgtca gctggggtag ctattttaaa aggagaattg
     4561 gcagatggcg actactgagt tgattgaact ggcaattgaa accagcaaac atgcatatgt
     4621 cccctattct cactttccta tcggagcagt tttagtagcc aaagacggaa gtgtttacac
     4681 gggagtgaat atcgaaaatg ctagctatcc tttgaccaat tgcggtgaga gaacagctat
     4741 ttttaaggct atatctgaag gccaaagaga attttcagaa ttgattgtct atggtcaaac
     4801 tgaaaaaccg atctcgccat gtggtgcttg tcgccaagtg atggctgaat tttttgaaca
     4861 agatctaaaa gtgaccttag tcgcaaaaga taaatcgacg gtcgagatga cggtcgggga
     4921 attacttcca tactctttta cagacttaaa ctagtctgag tcgctctctg agtggcacgg
     4981 tccttgtggc caaccaatcc atacttgcaa catcgttgca catcttattt aggaggttca
     5041 gtaatgaaca agaaacaatg gctaggtctt ggcctagttg cagtggcagc agttggactt
     5101 gctgcatgtg gtaaccgctc ttctcgtaac gcagcttcat cttctgatgt gaagacaaaa
     5161 gcagcaatcg tcactgatac tggtggtgtt gatgacaaat cattcaacca atcagcttgg
     5221 gaaggtttgc aggcttgggg taaagaacac aatctttcaa aagataacgg tttcacttac
     5281 ttccaatcaa caagtgaagc tgactacgct aacaacttgc aacaagcggc tggaagttac
     5341 aacctaatct tcggtgttgg ttttgccctt cataatgcag ttaaagatgc agcaaaagaa
     5401 cacactgact tgaactatgt cttgattgat gatgtgatta aagaccaaaa gaatgttgcg
     5461 agcgtaactt tcgctgataa tgagtcaggt taccttgcag gtgtggctgc agcaaaaaca
     5521 actaagacaa aacaagttgg ttttgtaggt ggtatcgaat ctgaagttat ctctcgtttt
     5581 gaagcaggat tcaaggctgg tgttgcgtca gtagacccat ctatcaaagt ccaagttgac
     5641 tacgctggtt catttggtga tgcggctaaa ggtaaaacaa ttgcagccgc acaatacgca
     5701 gccggtgcag atattgttta ccaagtagct ggtggtacag gtgcaggtgt ctttgcagag
     5761 gcaaaatctc tcaacgaaag ccgtcctgaa aatgaaaaag tttgggttat cggtgttgat
     5821 cgtgaccaag aagcagaagg taaatacact tctaaagatg gcaaagaatc aaactttgtt
     5881 cttgtatcta ctttgaaaca agttggtaca actgtaaaag atatttctaa caaggcagaa
     5941 aaaggagaat tccctggcgg tcaagtgatc gtttactcat tgaaggataa aggggttgac
     6001 ttggcagtaa caaacctttc agaagaaggt aaaaaagctg tcgaagatgc aaaagctaaa
     6061 atccttgatg gaagcgtaaa agttcctgaa aaataatgga tggaagtcat ttcttaggaa
     6121 gcggccaagg gccgcttttt taagagttga gacaaagtca ttttgtctca gtaaagcttg
     6181 ctactagaca aactagcaag ttttattgaa ataaaataag actctgaaag gaagagcaca
     6241 tggcacacga aaatgtcatt gagatgcgtg atattaccaa ggtgtttggt ggatttgttg
     6301 ccaacgacaa aatcaacttg cacctacgaa aaggtgaaat tcatgcactt ttaggagaaa
     6361 atggggctgg taagtccacg ctaatgaaca tgttagcagg ccttcttgaa ccaactagtg
     6421 gtgaaatcgc ggtcaacggt caagttgtca atctcgactc cccatctaaa gcagctagct
     6481 tgggaatcgg gatggttcac cagcacttta tgttggttga agccttcaca gtggctgaaa
     6541 acatcatttt aggtagtgaa ttgactaaaa atggtgtgct agatatcgct ggagctagca
     6601 aagaaatcaa ggctctttct gaacgttatg gcttagctgt tgacccttct gccaaggtag
     6661 cagatatctc agttggagcc caacaacgtg tagaaatttt aaaaacactt tatcgggggg
     6721 ctgatatcct tatctttgac gaaccaacgg ctgttttgac tccatcagaa attgatgagt
     6781 tgatggctat tatgaaaaat cttgtcaaag aaggaaaatc aattatcttg attacccaca
     6841 aattggatga aattcgcgca gtttctgacc gtgttacagt tatccgtcgt gggaaatcaa
     6901 ttgaaaccgt tgaaattgca ggggctacca atgctgattt ggcggaaatg atggtaggac
     6961 gttctgtttc ctttaaaaca gagaagcaag cctctaaacc aaaagaagtg gttttgtcta
     7021 tcaaagattt ggtggtcaat gaaaaccgtg gtgttccagc tgttaaaaat ctgtccttgg
     7081 atgttcgtgc tggagagatt gttggtattg cggggattga tggaaatggt cagtctgaac
     7141 tgattcaagc cattacaggt cttcgtaagg ttgaatctgg tagcattgag ctaaaagggg
     7201 attcaattgt aggcttgcac ccacgtcaga ttacagaact aagtgttggg cacgttccag
     7261 aagaccgtca ccgtgatggc ttgattttgg aaatgatgat atctgaaaat attgcccttc
     7321 aaacctacta taaagaacca catagtaaaa atggaatttt gaattattca aatattactt
     7381 cttatgctaa aaagctgatg gaagagtttg atgttcgcgc tgccagtgaa ttagttcctg
     7441 cagctgcact ctcaggagga aatcaacaaa aagcaattat tgctcgtgaa attgatcgag
     7501 atcctgatct ccttatcgtt agccagccaa ctcgtgggtt ggatgtcggt gccattgagt
     7561 atatccacaa acgcttgatt gaagagcgtg ataatggcaa ggctgtcctt gttgtcagct
     7621 ttgaattgga tgagatttta aacgtctcag accgtattgc cgttatccac gatggtaaga
     7681 ttcaaggtat tgtatcacca gaaacaacca ataaacaaga acttggtgtc ttgatggctg
     7741 gtggaaactt gggaaaggag aagagtgatg tttaaaaaat tacaacaaat ttcggttccc
     7801 ttgatttctg tatttctagg aattttactc ggagccattg tcatgtggat cttcggttat
     7861 gatgctattt ggggctacga agaattgttc tatacagcct ttggcagtct gcgtgggatt
     7921 ggagaaatct tccgtgctat gggtcctctg gtcttgattg gtcttggttt tgcggttgcc
     7981 agtcgagctg gtttctttaa cgtcggactt cctggtcagg ctttggcagg ttggattctc
     8041 agtggttggt ttgccctgtc gcatccagat atgccccgtc ccttgatgat tctagcaacc
     8101 atcgtgattg ccttgattgc tggtgggatt gtcggagcga ttccaggtat tcttagggcc
     8161 tatctaggga cgtcagaggt tattgtaacc atcatgatga actacattgt cttgtatgta
     8221 gggaatgcct ttatccatgc tttccctaaa gacttcatgc aaagtacaga ttcgaccatt
     8281 cgtgttgggg ctaatgcaac ctatcagaca ccttggttgg ctgagttgac tggtaactca
     8341 cggatgaata ttggtatttt ctttgccatc attgccgttg cagttatttg gttcatgctc
     8401 aagaaaacaa ctcttggttt tgaaatccgt gcagttggtc ttaatccaca tgcttcagaa
     8461 tatgctggta tttctgccaa gcggactatt atcctatcta tgattatttc aggtgccttg
     8521 gcaggtcttg gtggagctgt tgaaggtttg ggaaccttcc agaacgtcta tgttcaaggt
     8581 tcgtcattag ctatcggatt taacggaatg gcggttagtt tgcttgcggc caactcacca
     8641 attggtatac tctttgcagc cttcctattt ggcgttctcc aagttggggc tcctggtatg
     8701 aatgctgcgc aggtaccatc tgagcttgtc agcattgtaa cagcgtctat tatcttcttt
     8761 gtcagtgttc attaccttat cgaacgcttt gttaaaccga aaaaacaagt taaaggaggt
     8821 aagtaaagat gtctattata accttgctcc ccctcttggt gtcttctatg ctgatttact
     8881 cagcacctct catctttaca agtatcggtg gtgttttctc tgaacgtggt ggtgtggtaa
     8941 acgttggcct tgaaggaatt atggttatgg gtgccttttc tggagttgtc tttaaccttg
     9001 aatttgcaga acaatttgga gcagcaactc catggctatc cttgcttgta gcaggattgg
     9061 ttggtagtgt tttctctatc atccacgcag cagcgacggt tcatttccgt gcagaccatg
     9121 ttgtcagcgg tacggtattg aacttgatgg cgcctgcctt ggctgttttc ttagttaaag
     9181 ttctttataa caaaggacaa accgacaacc taagtcaaac ttttggacgc tttgatttcc
     9241 cagtcttggc aaatatccca gtgattggtg atatcttctt caagtcaact agtctacttg
     9301 gttatctggc gattgccttc tcattccttg cttggtttat tctctttaag actcaatttg
     9361 gtcttcgtct ccgctctgtc ggtgaacatc ctcaagcagc ggataccttg ggaatcaacg
     9421 tctacaagat gagatattta gggattatta tttcaggttt tctaggtgga attggcggag
     9481 cgatttatgc gcaatcaatc tcagttaact tctcagtgac aactattgtt ggacctggat
     9541 ttatcgccct tgctgcgatg atctttggga aatggaatcc aatcggtgct atgctttcta
     9601 gtctcttctt tggactttca caaagtttgg ctgttatcgg ttctcaattg ccgttcctac
     9661 aaggagtgcc agcggtttat cttcaaattg caccttatgt tttgacaatt cttgtcttag
     9721 cagccttctt tggaaaagca gtcgcaccaa aagcagatgg tatcaactac atcaaatcaa
     9781 aataagcata caaaaaaacg tcagttcaat gactgaactg ccccccaaaa gttagacaga
     9841 aaaaatctaa cttttggggt gtttttatta tgaaactaac ttatgatgat aaagttcaga
     9901 tctatgaact tagaaaacaa ggatatagct tagagaagct ttcaaataaa tttgggataa
     9961 acaattctaa tattaggtac atgattaaat tgattgatcg ttacggaata gagttcgtca
    10021 aaaaaggaaa aaatcgttac tattctcctg atttaaaaca agaaatgatt cataaagtct
//
LOCUS       DAVKQB010000065        10039 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKQB010000065 DAVKQB010000000
VERSION     DAVKQB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.065"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..674
                     /locus_tag="VW528_001865"
     CDS             <1..674
                     /locus_tag="VW528_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEU9912639.1"
                     /translation="KRIKRLRRISGGDTYTIIYLKLLLLSLKDEGKLYYDGVESDFIK
                     ELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTEIPNLIGSETAWAEKKRRYR
                     QNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYK
                     NLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYD
                     VGESL"
     gene            1358..1519
                     /locus_tag="VW528_001866"
     CDS             1358..1519
                     /locus_tag="VW528_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912640.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1611..3290
                     /locus_tag="VW528_001867"
     CDS             1611..3290
                     /locus_tag="VW528_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9912641.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            3558..4124
                     /locus_tag="VW528_001868"
     CDS             3558..4124
                     /locus_tag="VW528_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912642.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            4446..4634
                     /locus_tag="VW528_001869"
     CDS             4446..4634
                     /locus_tag="VW528_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9912643.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4696..5454
                     /locus_tag="VW528_001870"
     CDS             4696..5454
                     /locus_tag="VW528_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9912644.1"
                     /translation="MVVYIRQSKLPSEVSINKYHAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            5512..6366
                     /locus_tag="VW528_001871"
     CDS             5512..6366
                     /locus_tag="VW528_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9912645.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            6478..7362
                     /locus_tag="VW528_001872"
     CDS             6478..7362
                     /locus_tag="VW528_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9912646.1"
                     /translation="MKKVMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            7501..7602
                     /locus_tag="VW528_001873"
     CDS             7501..7602
                     /locus_tag="VW528_001873"
                     /inference="COORDINATES: protein motif:HMM:NF014902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEU9912647.1"
                     /translation="MNTLGDKLMELSAIYHRPESEYAYLYKDKKLHI"
     gene            7666..9264
                     /locus_tag="VW528_001874"
     CDS             7666..9264
                     /locus_tag="VW528_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEU9912648.1"
                     /translation="MEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTE
                     GQNILYGDKGCVENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFAN
                     GNALLNPEGTLDWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSN
                     HKYNTTDYFEIDRYFGDKETFRELVDQAHHRGMKVMMDAVFNHIGSQSLQWKNVVKNG
                     EQSAYKDWFHIQQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATY
                     WIEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHA
                     VMNYPLSDSIKDYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWT
                     ANEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNF
                     MKRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEA
                     VALASNCQELENQLVISPDGFVIF"
     gene            9539..9994
                     /locus_tag="VW528_001875"
     CDS             9539..9994
                     /locus_tag="VW528_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912649.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
BASE COUNT         3617 a         1393 c         2100 g         2929 t
ORIGIN      
        1 acaaaaggat aaaaaggtta aggagaatat ctggaggaga tacctatacg ataatctacc
       61 taaaacttct actactaagc ctaaaagatg agggcaaact ctattatgac ggagtagaaa
      121 gtgattttat aaaagagtta gcactaacca tagatgaaac agacgatgat gtaatggtaa
      181 cagtaaatta tttaatcaat caaggcttat tagaagttgt aacagaaaat gatgaatact
      241 acctaactga aataccaaac ttaatcggat cagaaacagc ctgggcagag aaaaaaagaa
      301 gatacagaca aaataaacag aggacattgt ccttaatgag tccaacccat gtccgacaag
      361 agatagagat agagaaagat atagagatag ataaagagag agagggagag atagataaaa
      421 agtccacccc cattttttat ggagaattca aaaatgtaag gttaagcaaa gaagaatata
      481 aaaatctaaa agaaaaatta aactcacaca cagatataat gataaataaa ctatccagat
      541 acatggaaag cagtggtaag acctatcaaa accactatgt gacaatctta aaatggtatg
      601 aagaagataa agacaaacta agacagaaag gtttaaataa aaaaatgaat tatgatgtag
      661 gagaatcttt atagataaaa agaggtggaa aggcactatt aaagacttta agtttaaaaa
      721 ggtatcaaag tatgccagag ataaaaaaat ataaatatga cactttaaag gcgattagaa
      781 aattaaagaa atgctagaag acttttagat gacagttacc aagagggtct aattttcact
      841 gctacttatg cagtaggaat tggacctttt tatataagca aattctattt caaaattggt
      901 taccaagaaa gacttctttt cactgttaag tataagaggg taatttatag aattttgtta
      961 catcaagata aatattttct gatggtcaaa ttttaaaaag attaataact ctcgataaat
     1021 tttgaacctt aacaattgaa tagaccaaga caagacgtta attatttttg gagcgtaata
     1081 acctatccgt caagatcttt cggctgaata attcggcaaa aaaagttctg ctaagcaaaa
     1141 agaaagtgag cgataagtaa gagtttgaat atagggaggg ataccctatc cgctatgaag
     1201 aaaatgagga taatgatact tctaaggttg aagccggctc atccggaata gaggcggagg
     1261 tgagattcct atgttgctaa tccaaagcac ttgtcaatgt caaaaaactt aattaaacaa
     1321 ataattatta gatataaaac tttagaggag gtatatattg gacaatgact ggaatggatt
     1381 ggctgattta atagcaaatc tgattgcaaa atatgctggt gctttagatt tagataatct
     1441 acccgatcca acgccagcaa aaaatcaaga gatgaaaaat agttttgaca tggcaaaaac
     1501 acaaattgag acggactaat aaaagtgtta taatatactt gaaatgatag tccaaactta
     1561 aattaaaaaa attatatatg atatagaaaa attatattga ggttaatact atgtcaaaag
     1621 aaaaaataaa agtatacctc tatacacgag tatctacgtc aatacagata gaggggtatt
     1681 cgttagaggc acaaaaatca cgaatgaaag cttttgctat ttacaacgat tatgaaattg
     1741 ttggagagta tgaagatgcc gggaagtctg gcaagtctat tgaaggtaga atacagttca
     1801 atcgaatgat ggaagatata aaatctggaa aagatggagt atcttttgtt cttgtgttta
     1861 agttatcaag atttgcaaga aatgccgctg atgttctttc tacacttcag ataatgcagg
     1921 attatggagt taatcttatc tgcgttgaag atggcattga ttcatctaaa gatgctggga
     1981 aactaatgat ttctgtttta tcagctgtgg ctgaaataga aagagaaaac atccgtattc
     2041 aaacaatgga aggttgcatt caaaaagcaa gggaaggaaa atggaatggt ggctttgctc
     2101 cgtatggata taaacttgaa gacggcaagc tgtttataaa tgaggaagag gcagttgcaa
     2161 taagaacgat tttcgaccag tatgtaaata ctacgatagg agccaatggg atatctaaat
     2221 acttagagaa tcatggaatt agaaaaatcc caagacagaa tggtaagaat cctttgtttg
     2281 atgcaggtct tataagaaag atattaaaga atcctgtata taatgggaaa atagcatttg
     2341 gaagaagaac tttagaaaaa gttcatggta caagaaatga atataagcag gttgaacaag
     2401 atgaatatct aatatctgaa gggatacatg aagctatagt ttccgatgaa gtttggcaag
     2461 ctgctcaggt taagctaaaa tctcaagcaa agaaatatga gcatgtgaat aaaggaaaag
     2521 atacacgcac acacttgctt tctggaattg taaaatgccc gatatgtgga gtgggaatgt
     2581 ttgggaacaa gtgtatcaag aaaaagaaag atggcacaaa gtataaagat ttttattact
     2641 atggttgtaa acataggcag atgataagag gtcataagtg tacattcagt aagcaaatta
     2701 gagaagaatt gttagatgat gctgttgcag aggtgattgt caagatagta agtaatccga
     2761 aatttgcttc tatgatgcaa gaaaaaatca acatgaaggt ggatacctct gaaatagaaa
     2821 aagaaataga taattaccaa aaggaattga gaaagagtca ttctacaaag tttaagctaa
     2881 ttgaggaaat agataattta gatgttgaag ataagcacta taagagaagg aaacaggatt
     2941 tagatgatag actatatcgt atgtatgaca aaatagatga attagaatca tcactaattg
     3001 atgcgaaagc aaagaaacag actattgaag ctgaaaaact tacaggagat aacatatata
     3061 aggttctgat ctattttgat aagctctata aagtcatgaa tgatgtagag cgtaggcagt
     3121 taatttcagc tttgatttct gaaattcaag tttatgaaga aaaacaatct aacggacaat
     3181 ggctaaaatc cattactttt aagctaccaa ttatagaaga aaatctcaac atagatttgg
     3241 acaatgatga acaagttgaa tgtgtaagtt tattagagaa acgtagttaa tcctcaaaag
     3301 gttccccaaa ctttttgagt cccactgacg catcacgctg aggcggtatc actgcttgta
     3361 cgagttgaag catcagcgaa gtaaaagtag atgttcagcc aatgcgacga ggtgagaccg
     3421 aaggtcgata aggtgtgttc tgggtatagg actcgtaaag taagtaggaa atatgatgag
     3481 atgatacaga gagcctgaat tggtcaggtg acgtgcttat ttacattagc ctgataatga
     3541 aaatggagag attatcgatg gttatattgg aattgtacca aaatgattat tctaaagatt
     3601 tagtagcatt tgattccata gaagatggga aagcctttgt ggctcaaatc ccgggataca
     3661 ctctggaaac agaagatggt tttgaagtgg agtattttaa tcctaaaaac ataccggatt
     3721 atatggaaat tatttttaac ggaaatattg ttcctttatc taaatttatg ttcgatcctg
     3781 aggaaaatgt aaacattatt tggaaagaga tttcaaattt atccctcaaa aatgatagag
     3841 tgatcgaagg atattcaaaa attgatgcat atgtcgttaa caaccatgaa gttaaagcct
     3901 atgtcgaaac aagagaaacg aactatcgta aagcaaaaga cttcttagaa agccgcggat
     3961 atgaaatcga cagaagcttt ttcggaagtg aagacggtga agcgattctt tacagaaaaa
     4021 aagggatcga agtttggcat ttcttgtgtc acttagaccc aatgtttgta gagattgaag
     4081 atgtagaagg atatgttaaa gaagaagttg gagagattca atagtcaacc aaagttcctt
     4141 gtgagtatat acctttgcta gtaaaagagt caaaacgtta taacgaaggg ggggaagaaa
     4201 acccttgagt gtaagtcttt ttcttgttta gagggaaagt attagagtag ccgttcataa
     4261 tttatactag atttgataac tgatttgaga cagattttat ttctataaat attgtgaaaa
     4321 ttttttaata ccaaagattt aaagttactg tgttaaaaat cctctccact tttaaactaa
     4381 tgtattataa aagccagttg acacaagtgt tacacctgtg atagaattaa cttgaggtga
     4441 tgaaaatggc aaccgataaa aatagaataa tgattagctt ggatgataaa aatctagaga
     4501 aacttgaaaa tttagtagaa gatgctagag atagaagggg aatgcgacta acaaagtccc
     4561 aggttattga attactccta aataccgttg attatttcga tgatattatg ggggctattt
     4621 actcaaaaaa ataagagcaa aattgctatt attttttaca aattggtgta acactagtga
     4681 tataggagtg atgaaatggt tgtttatatt agacaaagta aattaccaag tgaggtttca
     4741 attaataaat accatgctca agtaggtgcc tacttacaag gagaagaagc tgttttatat
     4801 caatcttttt ctgaaataaa agagttaaca agtgaagata tagttgtaga ttatataatg
     4861 gagactagag cattactaaa aatgatgggc ttaaacgttc cggttcatga ctatcctatt
     4921 gagcttaaag agttttatgg tcgaaagatt tatgctggta ttttaggaga gattgtgaat
     4981 atacctgata attggggaaa atttattaag cctaaagctg gttcaaaagt ctttactgga
     5041 agagttgtta atggaaccca tgatttaata ggtattggtc tacctttcga ctatcctata
     5101 tggattagtg aggttgtaga attcatagct gaatggcgtt gttttgtgtt agatggtcgc
     5161 gtattagatg ttcgacccta tacaggtgat tatcatgcac aatttgatgc aagtgtaatt
     5221 gatgaagcga tatcatgttg gaaagatgcg ccaatagctt atggactaga tatcggtgtt
     5281 actcgcgatg gcagaacact tgttgttgaa gtaaatgatg gttatgcatt gggaaattat
     5341 ggcctatctc ctttaaaatc aatcaatttt catagagcta ggtggaaaga aatggtaaaa
     5401 ccctattttg aaaaaaatga aatttttaaa atccaacagg atgttgtttt ctaaaataaa
     5461 tatggtatat aaccaatata aaaagcatca aaatgaacaa ggaggaatct gatgcttgga
     5521 gcaattattg gagatattgt cggttctgtt tacgaatgga acaatatcaa aacaaaggac
     5581 tttcctttat ttcggaagga ctgctttttt acagatgaca cggttatgac ctgtgctgtt
     5641 gcagaagcaa ttatgaatgg tggacaaaaa gacgatttca ttgacgcgat gaagaaatat
     5701 ggcagaatgt atccgaatgc tgattacggt gctcggttta atgcatggct aaacagcgat
     5761 aaccgtgagc cttataatag ctttgggaat ggatcagcta tgcgtatttc tccatgtgct
     5821 tggatcatgg actgtggttt ttatgcgaaa actggtatgt ggccatcatc tagaggactt
     5881 acgagtcttt ctgcagaggt aactcataat catccagaag gtgtcaaagg cgctatggct
     5941 acagctgatg ctatctttct atgtcgtttt tactttggtg gttattgtag agagtacgag
     6001 caatcaatta acgacaatcc tacagagtgt aaaagacgaa ttaaggatta tatagaaaaa
     6061 gaatacgatt acaatctatc tcaaacttta gatgaaatcc gtcctaacta tcgttttaac
     6121 gaaacatgtc aggaaactgt ccctcaagcc attatcgcct ttcttgaaag tagagacttc
     6181 gaagatgcga taagaaatgc catctcactt ggtggcgaca gtgatactct cgcagcaatc
     6241 acttgtaaca tagcagaggc agcttacggt attcctgatt ggatcaagga taaggcctat
     6301 tcttacttgg atgaaccctt aaaggatgta gttaggcgat gggaaaatag aataaaagcg
     6361 tattaatctc cagtacgtca gatttctctt ttctgtattt tcattgtata ataggattac
     6421 atttacttgc atacgacgaa atgtaaacat ttctgtttaa aattaaggat aaaatcaatg
     6481 aaaaaagtaa tggtaattat caaccctact tctggtggcg agaaggcttt ggattacaaa
     6541 gaaaagctgg agaataaagc aaaagaatac tttgaatatg ttgaaaccaa aattaccgaa
     6601 aaagcgctgg atgcaacaca ttttgctgaa gaagcttctc gtgagcagta tgatgcagtg
     6661 gttgtgtttg gtggagatgg aactgtcaat gaagtcattt caggtattga tgagagagac
     6721 tacattccta agttagggat tatcccaggc ggtacgggta acctcattac aaaacttttg
     6781 gaaatcaatc aagacatcga tggcgcaatt gacgaactgg attttgattt aaccaataag
     6841 attgatatcg gtaaagcaaa tgacaactat tttggttata tctttagtat cggttctctg
     6901 cctgaggcga ttcacaatgt tgaaatagag gacaaaacaa aattcggtat tttaacctat
     6961 gctgtaaata ccatgaagtc tgtcatgaca gatcaggtct ttaacattaa ggttgagaca
     7021 gaaaatggaa attatgttgg tgaagctagc catgttttgg tccttttgac aaattacttc
     7081 gctgataaga aaatctttga agaaaacaag gacggctatg ccaacatttt gattctgaaa
     7141 gatgcctcta tattctccaa attatccgtc attcctgatt tattaaaagg ggatgttgtc
     7201 gcaaatgata atatcgagta tatcaaagcg cgtaatatta aaatctcttc agatagtgaa
     7261 ttggagtcag atgttgacgg agataaatca gataacctac ctgtagaaat caaagtccta
     7321 gctcagcgag tagaagtatt ttcaaaaccg aaagaggatt agtatataga gaaagccttt
     7381 tttaaggctt tttgtatact ttaaaagata gttcctttaa caacggacat tccttgcaaa
     7441 tagtttacaa aaatagtata ctggatttat tgagtttgaa aacgtttgcg taaaatttga
     7501 atgaatactt taggagacaa attgatggaa ttgagtgcta tttaccatag gcctgagtcg
     7561 gagtatgcct atctttataa ggataagaaa ctccatattt gaattcgaac taagaaaggg
     7621 gacattgaaa gcatcaactt gcactatggg gaccctttta tctttatgga ggagttttat
     7681 caggatacaa aagaaatggt caagataact tctggtacct tatttgacca ttggcaggtt
     7741 gaagtgtcag ttgactttgc acgtatccag tatctctttg agctcagaga tacagaaggt
     7801 caaaatattt tgtatggcga taaagggtgt gtggaaaatt ctctagaaaa tcttcatgca
     7861 attgggaatg gatttaagtt gccttatctt catgagattg atgcctgcaa ggttcctgac
     7921 tgggtttcaa atacggtatg gtatcagata tttcctgaaa gatttgccaa tggcaatgct
     7981 ctattaaacc cagaagggac tttagactgg gattcatctg tcacacctaa gagcgatgat
     8041 ttctttggtg gtgatttaca ggggattatt gatcatatgg attacttgca agacttgggt
     8101 attactggac tatatctttg tcccatcttt gaatctacaa gcaatcacaa gtacaatacg
     8161 acagattact ttgaaattga ccgttatttt ggagacaagg agacctttcg ggaactggtg
     8221 gatcaagcgc atcatcgtgg catgaaagtc atgatggatg cggtatttaa tcatattggt
     8281 tcgcaatctc ttcaatggaa aaatgtcgtc aaaaatggcg aacagtctgc ttataaggat
     8341 tggttccata ttcaacaatt cccagtgaca actgaaaagc tagttaataa gagagactta
     8401 ccctatcatg tttttggttt cgaggactat atgcctaagc taaatacagc caatccagag
     8461 gtcaagaatt atcttttaaa ggttgcgact tattggattg aagagtttaa tatcgatgct
     8521 tggcgtttgg atgtggctaa tgagattgac catcagttct ggaaggattt tcgtaaggca
     8581 gttttagcta aaaatcctga tctttatatc ctaggagaag tctggcatac atctcagcct
     8641 tggctaaatg gagatgagtt ccatgccgtc atgaattatc ctttatctga tagtatcaag
     8701 gactatttct tacgaggaat taagaagaca gaccagttca tcgatgaaat caatggagag
     8761 tctatgtatt acaagcagca gatttcagag gtcatgttta atctcttgga ttcacatgat
     8821 acagagcgaa tcctgtggac ggccaatgaa gatgttcaac tggttaaatc agccttagcc
     8881 tttctctttt tacaaaaagg aacaccgtgc atttattacg gaaccgagct agccttgact
     8941 ggaggaccag atccagattg tcgtcgttgt atgccttggg aacgtgtatc aagtgacaat
     9001 gatatgctga actttatgaa gaggctgatt aagattcgga aatacgcgtc agtaatcatt
     9061 tcgcatggca agtatagcct tcaagaaatc aaatctgatc tagtagctct ggaatggaaa
     9121 tacgaaggac ggatcctcaa agcaatattc aaccaatcaa cagaagatta tcttttagag
     9181 aaagaagcag tagcactagc aagcaattgc caagaattgg agaatcagct tgtcatctct
     9241 ccagatggat ttgtgatttt ctaaaaacta gttgatgaag attatggtac atttcatatc
     9301 ttatatagta taataaggct agttactaaa cttgtaaagg agaacttaaa tgaattgtag
     9361 aggacatgaa acaagacaaa gaattgttag agattttgaa gtttagccta aagcacatat
     9421 taagctgtta gcaaatcaac aaaaacatag tgatgcagga gcaactattg aagatgaata
     9481 ttatgtattt atcgctgaga gtaaaattga tggcaagaag gaagttattc agtgttgcat
     9541 gggtgcggca agggattttt tagaactaat taatcacaaa gggctacctc tttttaatcc
     9601 gcttgtaggt gattctcatg taaataatag acaagaatat gacaatacag ggagtggaaa
     9661 tttacaacct gaaaagtgga atgaaactgc aaagcagctt tataatgcta taatgtggtt
     9721 gattatttta tggaatgcta agccggatac acctttattt aattttaaag acgaagtaat
     9781 taagtataaa acatatgagc cttttgaaag cagtataaaa agagtaaata ctactataaa
     9841 gaatggtagt aaagggaaaa ctctgactga gatgattaat ggctacagag cggataacga
     9901 tattagagat gaaatttgta actttaatat tctgaaaaat aaaattcgtg atatgaaaga
     9961 ccaacaagga aatacaatgg aatcttactt ttagttattg ttgaattttg ggtattctat
    10021 aaaatatcct aattgagat
//
LOCUS       DAVKQB010000066         9676 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKQB010000066 DAVKQB010000000
VERSION     DAVKQB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.066"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            180..2108
                     /gene="glgB"
                     /locus_tag="VW528_001876"
     CDS             180..2108
                     /gene="glgB"
                     /locus_tag="VW528_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9912650.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRKDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATNITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            2098..3240
                     /locus_tag="VW528_001877"
     CDS             2098..3240
                     /locus_tag="VW528_001877"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9912651.1"
                     /translation="MKNEMLALILAGGQGTRLAKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGIDSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLELGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            3230..4369
                     /gene="glgD"
                     /locus_tag="VW528_001878"
     CDS             3230..4369
                     /gene="glgD"
                     /locus_tag="VW528_001878"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9912652.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            4366..5799
                     /gene="glgA"
                     /locus_tag="VW528_001879"
     CDS             4366..5799
                     /gene="glgA"
                     /locus_tag="VW528_001879"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9912653.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            5858..6229
                     /locus_tag="VW528_001880"
     CDS             5858..6229
                     /locus_tag="VW528_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU9912654.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVPGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            6269..6502
                     /locus_tag="VW528_001881"
     CDS             6269..6502
                     /locus_tag="VW528_001881"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU9912655.1"
                     /translation="MISPQVKKETLEKWRKKLKLSKERTVAIGDGVNNLLMLKSAELG
                     IAFCSKEMLKKEIPHHVDKRDFLEVLPLIDCLE"
     gene            6511..7627
                     /locus_tag="VW528_001882"
                     /pseudo
     CDS             6511..7627
                     /locus_tag="VW528_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(7624..8070)
                     /locus_tag="VW528_001883"
     CDS             complement(7624..8070)
                     /locus_tag="VW528_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9912656.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            8234..9538
                     /gene="eno"
                     /locus_tag="VW528_001884"
     CDS             8234..9538
                     /gene="eno"
                     /locus_tag="VW528_001884"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9912657.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFLGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
BASE COUNT         2908 a         1725 c         2139 g         2904 t
ORIGIN      
        1 tagaaaaaga aaggtgtttt cgcaattttt tgaaaaaata ttgatttaca attgaacgag
       61 taagtgaact tgttgtagat tttcaaaata attaaagaag tatagtaatt tacttgaatc
      121 ttctctaaaa aggtagtata ataaaaacat agaaaacgct tacaggagaa agggagtata
      181 tggataatag agaagcatta aaaaccttta tgacgggtga aaatttttat ctccaacatt
      241 atctaggagc acatagggaa gaactaaatg gagagtatgg ctataccttc cgtgtttggg
      301 cacctaatgc tcaggctgtt cacttggttg gtgattttac caattggatt gaaaatcaga
      361 ttccaatggt aagaaaggac tttggggtct gggaagtctt taccaatatg gctcaagaag
      421 ggcatattta caaatatcat gtcacacgtc aaaatggtca tcaactgatg aagattgacc
      481 cttttgctgt caggtatgag gctcgtccag gaacaggggc aatcgtaaca gagcttcctg
      541 ataagaaatg gagggatgga ctttggctgg cacgaagaaa acgttggggc tttgctgaac
      601 gtcctgtcaa tatttacgag gttcatgctg gctcttggaa gagaaatcct gatggcagtc
      661 cttatagttt tgcccaattg aaagatgaac tcattcctta cctagttgag atgaactata
      721 ctcatattga gtttatgccc ttgatgtccc acccactagg tttgagttgg gggtaccagc
      781 ttatgggtta cttcgcttta gagcatgcct atggtagacc agaggagttt caagattttg
      841 tcgaggagtg tcatacccat aatattgggg ttattgtgga ctgggtacca ggtcacttta
      901 ccatcaatga tgatgcctta gcctattatg atgggacacc gacttttgaa taccaagacc
      961 ataataaggc tcataaccat ggttggggtg cccttaattt tgaccttgga aaaaatgaag
     1021 tccagtcctt cttaatttct tgcattaagc attggattga tgtctatcat ttggatggta
     1081 ttcgtgtgga tgctgttagc aacatgctct atttggacta tgatgatgct ccatggacac
     1141 ctaataaaga tggcggaaat ctcaactatg aaggttatta tttccttcag cgcttgaatg
     1201 aggttattaa gttagaatat ccagatgtga tgatgattgc agaagaaagt tcgtctgcga
     1261 ccaatattac gggaatgaaa gagattggtg gtctaggatt tgactacaaa tggaacatgg
     1321 gctggatgaa tgatatcctc cgtttctacg aagaagatcc gatctatcgt aagtatgact
     1381 ttaacctggt gactttcagc tttatgtatg ttttcaagga gaattatctc ttgccattct
     1441 cgcacgatga agtggttcat ggcaagaaga gtatgatgca taagatgtgg ggagatcgtt
     1501 acaatcaatt cgcaggcttg cgcaatctct atacgtacca aatttgtcac cctggtaaga
     1561 aattgctctt catgggtagc gaatacggtc aattcctaga atggaaatct gaagaacagt
     1621 tggaatggtc taacctagaa gacccaatga atgctaagat gaagtatttc acttctcagc
     1681 taaaccagtt ttacaaagat catcgctgtc tgtgggaaat tgataccagc tatgatggta
     1741 ttgaaatcat tgatgcggat aatcgagacc agagtgttct ttcctttatt cgtaagggta
     1801 aaaagggaga aatgttagtc tgtatcttta atatggtacc tgttgagcgg aaagatttta
     1861 caatcggact acccgttgca ggaatttacg aagaagtatg gaatactgag ttggaagagt
     1921 ggggaggcgt ttggaaagaa cataatcaaa ctgttcaaac gcaagaagga ctatggaaag
     1981 attatgagca gaccttaacc tttaccctac cggctatggg agcaagtgta tggaaaatca
     2041 aacgtcgctt gaaatctact aaaaccgtca caaataaaaa ccaaaaagga gtagaaaatg
     2101 aaaaatgaaa tgttagcttt gattcttgct ggtgggcaag gaactcgtct cgctaaactc
     2161 actcaaagca tcgcaaaacc agctgtgcaa tttggtgggc gctaccgtat cattgacttt
     2221 gccctatcaa actgtgccaa ctcagggatt cacaatgttg gggtcgttac acagtatcaa
     2281 ccacttgctc tcaacaacca tattgggaat ggttcaagct ggggactaga cggtattgat
     2341 tcaggtgtct ctattcttca accttattct gcaagtgaag gaaatcgttg gtttgagggg
     2401 actagtcacg ctatttacca aaatatcgac tatatcgaca gtgtcaatcc tgagtatgtc
     2461 ttgattttgt ctggggatca catctacaaa atggactatg atgatatgct ccagtctcat
     2521 aaggataata atgccagctt gacagtagca gttttagacg tccctcttaa agaagcaagc
     2581 cgttttggta tcatgaacac agatgctaac aatcgtattg ttgaatttga agaaaaacca
     2641 gctcaaccta aatctacaaa agcttctatg ggaatctaca tttttgattg gcaacgcctt
     2701 cgtaatatgt tagtcgctgc tgaaaagagc aaggttggca tgtcagactt tggtaaaaat
     2761 gtcattccaa attaccttga gttaggtgaa agtgtttatg cttacgaatt tagtggttat
     2821 tggaaagatg ttggtactat tgagtcactt tgggaagcga acatggagta tatttctcca
     2881 gaaaatgcct tggatagtcg taaccgtcaa tggaagattt actcaagaaa cttgatttca
     2941 ccaccaaact tcctcggggc aaatgctcat gtggaagact cattagttgt agacggatgt
     3001 ttcgttgatg gaactgttaa acattctatc ctttcaacag gcgcgcaagt tcgcgaagga
     3061 gcggaagtcc ttgattcagt tatcatgagt ggagctatca ttggtcaagg agctaagatt
     3121 aaacgtgcca ttattggtga aggtgcgatt atttctgacg gtgtcgaaat tgatggaaca
     3181 gatgaagtac aagttgtagg atataatgaa gtagtggggg tagcaacaga tgaagattga
     3241 taaatattct gccattttag gaaatacagt tggttttcac aatatgtcga cattgacgga
     3301 ccaccgtcca gtagcaagtt tgccatttgg tgggaaatat cgtttgattg atttcccact
     3361 ttcaagcctt gctaatgcag gtgttcgtag tgtctttggt attttccagc aggataatat
     3421 cagctcagta tttgaccata ttcgttcagg acgcgagtgg ggcttatcaa cccttcttag
     3481 ccattactat ctaggaattt acaatacccg tgtagaaagt agtacagttg gaaaagaata
     3541 ctaccaacag cttcttactt atttgaaacg ttctggctca aaccaaacgg ttgccctcaa
     3601 ctgcgatgtt cttattaaca ttgatttgaa ccaagttttc cacctacata gtacaacaaa
     3661 agagcctatc actgtagttt ataaaaaact agctaagaaa gatatttcag aagtaaatgc
     3721 aatcttggat gtagatgaaa cagaccatgt tctttctcat aaactctttg atagcaagtc
     3781 aacagctgaa acgttcaata tgtctacaga tatctttgtc gttgatacac cttggttgat
     3841 tgaacacttg gaagaagaag ctaaaaaaga acatccagag aaattgcgct atgttttacg
     3901 ggatttggct gtaaaagagg gagctttcgc ctacgagtac acgggctatc tagccaatat
     3961 tcactctgtt aaatcttatt atcaagcgaa tatcgatatg cttgaatcac aaaaattcta
     4021 ttctcttttc tcaccaaacc aaaagattta tacaaaggtc aaaaacgaag agccaactta
     4081 ctatgctaat acatctaagg taagcacttc tcagtttgcc tctggtagta ttattgaagg
     4141 tcaagtagct aattctgttc tatcacgtaa tattcatgtc cataaggata gcttggttaa
     4201 agatagcctg ctcttccctc gtgttgttat tggagaaggg gctcaggtcg aatatgctat
     4261 cttggacaaa ggggttgaag ttgagcctgg tgttgtgatt cgaggaactg cagaacatcc
     4321 agttgtggtt aagaaaggtg ctaaagtaac agaggatatt cattcatgaa aattttattt
     4381 gtagcagcag agggtgcacc cttttcaaaa acaggtggtt tgggagacgt cattggcgct
     4441 cttccaaaat cactggtaaa agctgggcac gaagttgcag tgattttacc ctactatgat
     4501 atggtagagg ctaaatttgg aaatcagatt gaagatgtgc ttcattttga ggtgagcgtt
     4561 ggttggcgca gacagtattg tggaattaag aaaacagtat taaatggtgt aaccttctac
     4621 tttattgaca atcaatatta tttcttccgt ggtcatgttt acggtgattt tgatgacgga
     4681 gaacgctttg cctttttcca actggctgcc attgaggcta tggaaaggat tgactttatt
     4741 cctgatcttc tccatgttca tgactaccat acagctatga ttcctttctt gttgaaggaa
     4801 aaataccgtt ggattcaagc ctatgaggac attgaaacag ttttaaccat tcataattta
     4861 gaattccaag gacaattttc agaaggaatg ttgggtgatt tgtttggagt tggctttgaa
     4921 cgttacgctg atggcaccct tcgatggaac aactgtctga actggatgaa ggcaggtatt
     4981 ctctatgcga accgtgtttc aaccgtttca cctagctatg ctcatgaaat tatgactagt
     5041 cagtttggat gtaatttgga tcagattctt aaaatggagt ctggtaaagt atctggtatc
     5101 gtgaatggga ttgatgctga tctttataat cctcagacgg atgctctttt agactatcat
     5161 ttcaatcagg aagatttgtc tgggaaagcc aaaaataagg caaaattgca agaaagagtt
     5221 ggcttgcctg ttagagcaga cgttccactg gtgggaattg tttctcgttt gacacgtcaa
     5281 aaaggttttg atgtggtggt cgaaagtctt caccatatct tgcaagaaga tgttcagatt
     5341 gttcttttgg gaactggcga tccagccttt gaaggagctt tctcatggtt tgctcagatt
     5401 tacccagaca agctatcaac aaatatcact tttgatgtca aacttgctca ggaaatctac
     5461 gctgcttgtg acctcttcct catgccaagt cgttttgaac cgtgtggctt gtctcaaatg
     5521 atggctatgc gttatggaac cttgccattg gtccatgaag ttggaggctt gcgagataca
     5581 gttcgcgctt tcaatccaat cgaaggaagc ggtactggct ttagctttga caatctatct
     5641 ccttattggt taaattggac tttccaaaca gcattggact tgtatagaaa ccatccagac
     5701 atttggagaa acctacaaaa acaagctatg gagagtgact tctcatggga tacagcctgc
     5761 aagtcatacc ttgacttgta ccatagttta gttaattaat agataaaatc gtatgatgac
     5821 ttcatacgat ttttaggctg tttagagagg agaactgatg tctcaagtaa aaggcttgtg
     5881 tgttttggat gtagatggaa ccttaatcct agaagaagtg attgatcttt tagggagaga
     5941 ggcaggtcat gaggcggaaa tttcgcagat tacaagtcga gcaatgcgag gagagttggt
     6001 ctttgaaagc agtttaagaa aaagagtgtc cttgttggaa ggtcttccta ttttggtctt
     6061 tgataacgtc ttcaactcaa ttcatctatc gctaaatgtc ccagagttca tctctattct
     6121 ccaaaagaat ggcatcctag ttggtctggt gcctggtgga tttacaccaa tagttggaga
     6181 gattagcaaa atcccttggt attgcctatt tcactgccaa ccagcttgaa gtcaaagaag
     6241 gtttttaaca ggaaaattag ttggacaaat tataagtccc caggtcaaaa aagaaactct
     6301 ggaaaaatgg agaaagaaac taaaactttc taaagaaaga acggtggcaa tcggtgatgg
     6361 ggtcaataat ctattaatgt tgaagtcggc ggagttagga atcgcctttt gttccaaaga
     6421 aatgctcaaa aaagaaatac cacatcatgt tgacaagagg gattttttag aagttcttcc
     6481 tttgattgac tgtttagaat gagggaaaat atgaagattg taattgcacc ggattcgttt
     6541 aaggaaagct tgacagctca acaggtagct gaagcaataa aaagaggctt ccaacaatcg
     6601 atagcagatg tagaatatct cctctgccct gttggtgatg ggggagaagg tactgtagat
     6661 gctatccgac attctcttga cctaaaagaa aaatggatcc aagtgacaga cccttttgga
     6721 caaaaagaag ccatgcgcta ttttcaaaaa ggggaactgg cactatttga agtagctgac
     6781 ttggttggtc ttggaaaaat tccgctagag aaacgaaatc cacttcaaat ccaaacttgt
     6841 ggtattggag agttgattct ccatctcatt tctaaaggga ttaaagatat ttatatcggt
     6901 gttggtagca cggccagtaa tgatggagga ctggggattg ctgctggttt aggttatcaa
     6961 ttttatgata gggatggaaa tgtcttgcct gcttccagtc aatccttatt aaacttagct
     7021 tctgtttcaa cagaaaattg ctataaaatt cctgaaggtg ttcaaattca tattctagca
     7081 gatgtcgtga gtcccttatg tggtcatcaa ggtgcgactt acacttttgg caatcaaaaa
     7141 ggtctacatc cgactatgtt tgcagtcgta gatcaggcga tccaagattt ttatgaaaaa
     7201 ttctcacctg caacattaga aattaaagga gcaggagcag gtggaggcct tgctggtggt
     7261 ttgtgtgcct ttgctcaggc aagtatcgtg tctggaattg ataccttgct tggacttaat
     7321 caactttgat aagaaagttt cagatgctga cttgcttgtt gttggagaag gaagacttga
     7381 cagtcaaagc tttgctggga aagcgcctat aggtgttgca aaaagaaccc ctgttggagt
     7441 tcctgttatt gctatttgcg gtagtcttgc tgaagattta cctcccctac catttgaaaa
     7501 tatccaagct gtcttttcta ttttggagaa aagtgaacct ttagaagata gtttgaaaaa
     7561 tgccagtctc tatctggagc gcacggctgc caatattggg cacttattaa atatgtgcaa
     7621 gatttagcta aaccatttct tccataagga tgttttggct ggttcttcct ttttaacttc
     7681 ccaaagtttt ggaaaagcaa gtcgaaggtc tttttcttct acttgaactg gtgcattgct
     7741 atgaataacc aggccaaaag gagaagtaat atgctcttca gatactatgg tggcttgact
     7801 atcagtttct tttgcttctt ttaagtagaa aacttgctta tcaaattcga tagttggtga
     7861 aatcttcaca aatagtggct ctgctttttc ctgaaggttt tctaaaataa ataaaaagcc
     7921 tttttctaac tgagggctat ttgctgtgtc aatatctaca tatccaagaa ctctttcctc
     7981 aaaagtacca agatagcgtc tttgctcatc cggatttatt tttaacccac catgagcttt
     8041 ttcaagtaat tgttttgata aatctgtcat gaaaacagta tatcataaaa gttagaataa
     8101 aacagacaaa agaaagcgat tactttataa agttaaggaa aagttgcaca aagataacgt
     8161 tttttcttga aaaaggaggg gttttaaggt attatagagg tgtaaaaaat ttaactcata
     8221 aggagagtaa aaaatgtcaa ttattactga tgtttacgct cgcgaagtcc tagactcacg
     8281 cggtaaccca acacttgaag tagaagttta cactgaatca ggtgctttcg gacgtggtat
     8341 ggttccatca ggagcttcta ctggtgaaca cgaagcagtt gaacttcgcg acggtgacaa
     8401 atctcgttac ggtggtcttg gtacacaaaa agctgttgac aacgtaaaca acatcattgc
     8461 tgaagctatc attggctacg atgtacgtga tcaacaagct attgaccgtg ctatgatcgc
     8521 acttgacggt actcctaaca aaggtaaatt gggtgcgaat gcaatcctcg gtgtgtctat
     8581 cgctgtagct cgtgctgctg ctgactacct tgaaatccca ctttacagct accttggtgg
     8641 attcaacact aaagttcttc caactccaat gatgaacatc atcaacggtg gttctcactc
     8701 tgacgctcca atcgctttcc aagagttcat gatcttgcca gttggtgcgc caacatttaa
     8761 agaagccctt cgttacggtg ctgaaatctt ccacgctctt aagaaaatcc ttaaatcacg
     8821 tggtttggaa actgccgtag gtgacgaagg tggattcgct cctcgtttcg aaggaactga
     8881 agatggtgtt gaaactatcc ttgctgcgat tgaagctgct ggatatgtac caggtaaaga
     8941 cgtatttctc ggatttgact gtgcttcatc agaattctac gataaagaac gtaaagttta
     9001 cgactacact aaatttgaag gcgaaggtgc tgctgttcgt acatctgcag aacaaatcga
     9061 ctaccttgaa gaattggtta acaaataccc aatcatcact attgaagatg gtatggatga
     9121 aaacgactgg gatggttgga aagctcttac tgaacgtctt ggtaagaaag tacaacttgt
     9181 tggtgacgac ttcttcgtaa caaacactga ctaccttgca cgtggtatcc aagaaggtgc
     9241 tgctaactca atccttatca aagttaacca aatcggtact cttactgaaa cttttgaagc
     9301 tatcgaaatg gctaaagaag ctggttacac tgctgttgta tcacaccgtt caggtgaaac
     9361 tgaagattca acaatcgctg atatcgcagt tgcaactaac gcaggacaaa tcaagactgg
     9421 ttcactttca cgtacagacc gtatcgctaa atacaaccaa ttgcttcgta tcgaagacca
     9481 acttggtgaa gtagctgaat atcgtggatt gaaatcattc tacaacctta aaaaataaaa
     9541 tagttcagtg aactatttta tcccgaacca tgaaattcaa aagttcggcc gttgatttaa
     9601 caacgtttct agcccctcgg attttatccg aagggctatt tttgtattta gagggcaaaa
     9661 aagggacaaa actttt
//
LOCUS       DAVKQB010000067         9508 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKQB010000067 DAVKQB010000000
VERSION     DAVKQB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.067"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(136..1272)
                     /gene="lctO"
                     /locus_tag="VW528_001885"
     CDS             complement(136..1272)
                     /gene="lctO"
                     /locus_tag="VW528_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9912658.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1897..3387)
                     /gene="lysS"
                     /locus_tag="VW528_001886"
     CDS             complement(1897..3387)
                     /gene="lysS"
                     /locus_tag="VW528_001886"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9912659.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINKPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3515..3661)
                     /locus_tag="VW528_001887"
     CDS             complement(3515..3661)
                     /locus_tag="VW528_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9912660.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TSFQ"
     gene            3694..4611
                     /locus_tag="VW528_001888"
     CDS             3694..4611
                     /locus_tag="VW528_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275026.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU9912661.1"
                     /translation="MYIAVWGSLGSFLLGLIVSIIRHYRIPVLAQVATAYIELSRNTP
                     LLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSAMQDSGIQVLFQGNNLLRILQGL
                     GVTIGISILSVLLSMMFGTVMGIIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFG
                     LARNFNINISGETSVIIVFTLWGTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYY
                     HIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTTSFW
                     IYGTILILYFAVCYPISKLSTHLEKHWRN"
     gene            4612..5370
                     /locus_tag="VW528_001889"
                     /pseudo
     CDS             4612..5370
                     /locus_tag="VW528_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            5382..6242
                     /locus_tag="VW528_001890"
     CDS             5382..6242
                     /locus_tag="VW528_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9912662.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAK
                     ADDLVVEGGH"
     gene            complement(6322..6969)
                     /locus_tag="VW528_001891"
     CDS             complement(6322..6969)
                     /locus_tag="VW528_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912663.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKPGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(6966..7664)
                     /locus_tag="VW528_001892"
     CDS             complement(6966..7664)
                     /locus_tag="VW528_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912664.1"
                     /translation="MGKLSSYMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            complement(7674..8426)
                     /locus_tag="VW528_001893"
     CDS             complement(7674..8426)
                     /locus_tag="VW528_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912665.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(8426..9190)
                     /locus_tag="VW528_001894"
     CDS             complement(8426..9190)
                     /locus_tag="VW528_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912666.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(9286..9508)
                     /locus_tag="VW528_001895"
                     /pseudo
     CDS             complement(9286..9508)
                     /locus_tag="VW528_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3066 a         2000 c         1657 g         2785 t
ORIGIN      
        1 attgttagaa atcgatttaa ctgtcctgat taatttgtcc tattcttatt tcattttatt
       61 atagttttcg atagcgattt attcttccaa tacacgaaga aaaacctcca cattcagtgg
      121 aggcaatctg ttttatcaat acaattttaa gtcacgaggg tcaactggga aggttgggtt
      181 gtatggattg tgacggagct taaagtgttt gacatcttca atggtctgag ctccagacaa
      241 ttgcataact gtcttcaatt ccgcattcaa gtgttcaaag acttgacgca caccgacact
      301 accaccgaga gccaagccat aaataacagg gcgaccaata gctaccaagt ctgctcctga
      361 agccaaggct ttaaagacgt gttgaccacg acgaacacca gagtcaaaga caatcggcac
      421 acgtctatca actgcttctg ccacttcttg aagcgagtca aaggcagctg gtccaccgtc
      481 gatttgacga ccaccgtggt tggttaccca gataccagaa gctcctgcag caagtgaacg
      541 ttcaacgtcc tcacggcatt gtggtccctt gacatacaca ggaagaccag agtattcagc
      601 gataaattct acatcgcgtg gagacaagcg ttgtttagct gatttgtaaa caaagtccat
      661 tgatttacca gcaccttctg gcaggtattc ttcaacaatc ggcatgccaa ctgggaagac
      721 aaaaccatta cgcttatcca cttcacgatt cccccctaca gtagcatctg ccgtcaagac
      781 aatcgcttta taaccttcag ccttcacacg gtccatgatg tggcggttga taccgtcatc
      841 cttactaaag taaaattgaa accaatgagg tgtcccttga agggcttcag aaatctctgg
      901 aaggtcaaca gtagagtaag aactggttgt ataaagagaa ccaaactcat gcacaccacg
      961 cgcagtcgcc acttccccct gttcattcgc taatttatgg gccgcaacag gcgccataat
     1021 gattggtgaa gatagttttt cacctgcaaa ttcaatctct gtacttggat tttcaacatc
     1081 gcaaagtgta tgaggaacga tgagtttgtg gttaaaggcg cggatattct ctcttaaagt
     1141 gaaagtatct tccgccccac tagcgatata gccaaatgct gctttaggaa taacttgttg
     1201 cgccattggc tccaaatcat aggtattgat gaagtctaca tgaccttctg cattgcttgt
     1261 tttgtatgac ataaaatgcc ctccttgatt aagtaagcgt ttgcttttta ctacacaact
     1321 ataataactc ttttttcaat cattttcaag agttttgctt aaaaaccatc ttattttttc
     1381 atacaatata ctagccctac tttccttaaa ctctaaactt tagtctatat taagtgaatc
     1441 gttgagattt tttgctgcca ggcatttgaa ctactttttt atttgcggat tctatctact
     1501 aatactataa aaaaacaagt gaataagaca ccagaacatc ctctaatagg acctatggac
     1561 ttatatcagt cccatatata ctgtttaagc aagatactat cctagttgac gctaacttcc
     1621 tatcccactt attaattttt tatttatagt gaatctgaac aaatcaattg ggaaatccaa
     1681 gtcaatttct agtacttttt gaacagtttc agcgtactat cgtaaaatga aataaaacat
     1741 gcacaaattg attagggaat ttaaaccaat ttctaacagt gtcttagaaa tcaaagtgta
     1801 ctatttcaac ttcaatatac tacacttcat tttctcctta aattcagtct ctttttgaat
     1861 caaaaaatcc cctggtaaga gccagagggt aagaatttat ttcattgttg ggaagagcaa
     1921 tacatcacgg atagttgttg tatcagtgag gagcatgcag agacggtcga taccgattcc
     1981 caaaccacct gttggtggca taccgtattc aagagcctcg atgtagtcat agtcgatgcc
     2041 tgtcgcttca tcatcaccaa gttctttagc tttagcttgg gcttcaaaac ggctaagttg
     2101 atcgattggg tcgttcaact cagtaaaggc attaccgtac tccttagtca tgataaagag
     2161 ctcgaaacgg tcagtaaagc gttggtcttc aggatttttc ttagcgagtg gagatacagc
     2221 tactggatgt ccatagacaa aggttggttg gattaaagtt tcttcgacaa actcttcaaa
     2281 gaaggcattg atgatgtgac caacctcagt gtagtgtttc tcaactggaa ctttcttctc
     2341 agcagcgata gctttagctt cttccaaagt catgtcttgc cagaaatcga caccagtaat
     2401 ttctctgata gcatccacca tatgaacacg cttaaatggt ttgttaatct tgatttcagt
     2461 accttggtag ttgactgggc catcaccttt gactgattta gcagcgtgtt ggataatgcc
     2521 ttccgtcaag tccatgatgt cttggaaatc tgcataagct tggtaaactt cgatagaagt
     2581 aaactcaggg ttatgagtag cgtccattcc ttcgttacgg aagatacggc caatttcata
     2641 gacacgttcc ataccaccca cgataaggcg ttttaagtga agctcagtcg cgatacgaag
     2701 caccatgtca atgttttggg cattgtggtg ggtgataaat ggacgggcag cagcaccacc
     2761 ggcttcatta tgaagaacag gtgtttccac ttcaaggaat cctttttggt caaggtaacg
     2821 acggatttca gagatgattt ttgaacgagt gacaaagcgt tcaaagcttt cacgattaga
     2881 aatcaagtca aggtaacgtt tacggtaaat tgtttcaacg tctgtcaaac catggaattt
     2941 ctcaggaaga ggacgaagag ccttagacaa gtgtgtgatg tgggttgcct tgatagagag
     3001 ttctcccata tccgtacgca tcacttcacc ttcgacacca aggaagtcac caaggtctgc
     3061 ttttttgaag atttcgtagt tttcttcacc gacagcatcc ttacgaacat agatctgaat
     3121 ctggccttcg cggtcttgaa ggtgggcaaa accaactttt cctttaccac gtttggttac
     3181 caagcgtcct gcgatagtag ctgtttcgtt tttatcgtgt aattgttctt tatcgaggtt
     3241 ggcatattta tcttttaatt cttgtgaatt tgcagtacgt tcaaaacgtt ttccgaaagg
     3301 atcgattcct tgttcgcgga gcgcagccat tttttcacgg cgaacgatct gctggtcatt
     3361 tagttcttcc atatgttctg tagacatggg atcctcctag ttttactcaa aattgaatac
     3421 gatgagtcat tttttgcgat acccccattt tatcataaat aagcaagaaa ttcacggata
     3481 atctttaaaa actaaaaaga acctgacgct aaaatcactg gaaagaagtg aaagtatctt
     3541 ccgccccact agcgatatag ccaaatgctg ctttaggaat aacttgttgc gccattggct
     3601 ccaaatcata ggtattgatg aaatctacat gaccttctgc attgcttgtt ttgtatgaca
     3661 taaaatgccc tccttgataa gtaagcgttt actttgtata ttgcagtttg gggaagtttg
     3721 ggatcctttc tgctcggttt aatcgttagt atcatccgac attatcgaat ccctgttttg
     3781 gcgcaagtag cgacagccta cattgaattg tcacgtaata cgcccctttt gattcaactc
     3841 ttctttctct acttcggtct tccccgaatc gggattgtcc tatcttcaga agtctgtgca
     3901 acgcttgggc ttgtcttttt aggaggctcc gccatgcagg attcgggaat ccaagtactc
     3961 tttcaaggaa ataacctcct gagaatctta cagggattgg gcgttacgat tgggatatcc
     4021 atcctgtctg tcctcttatc catgatgttc ggaacagtca tgggaatcat catgacctcc
     4081 cattctagaa tcatacgatt tttaacacga ttgtatctgg aatttatccg tatcatgccc
     4141 cagctggtgc tactcttcat cgtttacttt ggcttggctc gaaactttaa tatcaatatc
     4201 tcaggtgaga cttcagttat tatcgttttt accctctggg gaacagctga aatgggagac
     4261 ttggtacgtg gagctatcac ttctctccct aaacatcagt ttgaaagtgg acaggcactc
     4321 ggcttgacta atgttcaact ttactaccac atcatcatcc cacaagtctt gagaagactg
     4381 ctaccgcagg ctatcaatct tgtcactcgg atgattaaaa ccacttcatt agttgttttg
     4441 attggggttg tggaagtgac caaagttgga caacaaatca tcgatagcaa tcgcctgacc
     4501 atcccaacta cttcattttg gatttatgga accattctaa tcttatattt cgcagtttgc
     4561 taccctattt ccaaactatc cactcactta gaaaaacatt ggagaaacta aatgtctgaa
     4621 actatcttag aaatcaagga actaaaaaaa tccttcggag acaatcccat cctccaagga
     4681 ctttctctag aaatcaaaaa aggggaagtt gttgtcatcc tagggccatc tggttgtggg
     4741 aaaagtaccc tccttcgttg cctcaacggc ttagaaagta ttcaaggtgg agatattctt
     4801 ctggatggtc agtctatcgt tgaaaataaa aaagattttc acctagttcg ccaaaagatt
     4861 ggcatggtct tttaaagtta tgaactcttt ccccatctgg atgtcttaca aaacctcatc
     4921 ctaggcccta tcaaaactca aggatgggac aagaaagaag taacggaaga agctttgcaa
     4981 ttactagatc gtgtcggttt gctggataaa caacatagct ttgcccgtca attatctggt
     5041 ggacagaagc aacgtgttgc aattgtccgt gccctcctaa tgcatccaga aatcatcctt
     5101 tttgacgagg tgactgcttc gctggatcca gaaatggtgc gtgaggtgct ggaacttatc
     5161 aatgatttgg cccaagaagg ccgtaccatg attttagtaa cccacgaaat gcagtttgcc
     5221 caagccattg ctgaccggat tatcttcctc gaccaaggga aaatcgctga agaaggaaca
     5281 gctcaagcct tctttaccaa tccgcaaacc aaacgagccc aggaattttt aaacgtcttt
     5341 gactttagcc aattcggctc atatctataa aggagattct tatgaaacta ttcaaaccac
     5401 tcttaactgt tttagcactt gcctttgccc ttatctttat cactgcttgt agctcaggtg
     5461 gaaacgctgg ttcatcctct ggaaaaacaa ctgccaaagc tcgcactatc gatgaaatca
     5521 aaaaaagcgg tgaactgcga atcgccgtgt ttggagataa aaaaccgttt ggctacgttg
     5581 acaatgatgg ttcttaccaa ggctacgata ttgaactagg gaaccaacta gctcaagacc
     5641 ttggtgtcaa ggttaaatac atttcagtcg atgctgccaa ccgtgcggaa tacttgattt
     5701 caaacaaggt agatattact cttgctaact ttacagtaac tgacgaacgt aagaaacaag
     5761 ttgattttgc ccttccatat atgaaagttt ctctgggtgt cgtatcacct aagactggtc
     5821 tcattacaga cgtcaaacaa cttgaaggta aaaccttaat tgtcacaaaa ggaacgactg
     5881 ctgagactta ttttgaaaag aatcatccag aaatcaaact ccaaaaatac gaccaataca
     5941 gtgactctta ccaagctctt cttgacggac gtggagatgc cttttcaact gacaatacgg
     6001 aagttctagc ttgggcgctt gaaaataaag gatttgaagt aggaattact tccctcggtg
     6061 atcccgatac cattgcggca gcagttcaaa aaggcaacca agaattgcta gacttcatca
     6121 ataaagatat tgaaaaatta ggcaaggaaa acttcttcca caaggcctat gaaaaaacac
     6181 ttcacccaac ctacggtgac gctgctaaag cagatgacct ggttgttgaa ggtggccatt
     6241 aactcttaaa agggactgga ttttaagctc caatcccttt ttaagatttt acctataaca
     6301 tcctgagtct atctaagatg ttcaatctga acacagtgta catactttat cttctattgc
     6361 atatacttta tcacataaga tacgaatatc ctcttcacta tgactagcaa tcaaaattgt
     6421 tgtccctttt tcactagaga gctttctaaa caatgttctc atattttcta cacttgattt
     6481 atccaaggca ttcataggtt catctagtaa aagaatagag ggattctcca taattgcttg
     6541 agcaatccct agctttttcc tcatacctag cgaataagtt ttaactttct ggtctttttg
     6601 ctcatataga ccaactattt tcagtgtatc attgatttcc tgattaccaa ctactcctcg
     6661 tatgcttgcc aaatattgta aattcttaaa gccactataa taatttataa aaccaggttc
     6721 ttcaatcaaa gctcccaaat tagctggaat ttttctctca ggaacaatat tttccccatt
     6781 gattaacact tctccataag acggactata taaaccagct attaatttaa acaatacact
     6841 tttccctgag ccattcgcac cagtgattcc tatgatttcc cctggtttac aactaaagtt
     6901 aaggttttga aaaacacatg tcttttttaa tttcaactca atatttttta atatgattat
     6961 ttcattcatt ctataaacct cctcttttga cgagtgaaat agaaaatact tttaaaaaga
     7021 aagactaaaa atagcaactg aagaaataaa tctcgtccta tatctccatt ccctcgattc
     7081 aaaatataaa atagataatt agttcgattc cctacaaata gaccaccaaa cacaatcatg
     7141 agtaaaaaga aactaacgca agcaaagttc atttgtacat tcgctcctag tgcgtataac
     7201 ataaagcaaa gcaaggatag cacacaagca ttcaaagaat caactataaa caaaactcct
     7261 atatcccagt ttggtaactg taacataaat tgaatcacta gaacttttac catgattaaa
     7321 atgagtccat aaagaaaaca ggtccaaatc tgatacctaa tccagagctt aatagaagaa
     7381 aatcgaatca cttccataaa gaaatcggat gctcttctac gatatacaac tgctagaaaa
     7441 aatgtatgaa taataaacca atgaagcaac catatggata agactaaaaa agagttttga
     7501 ctcagattag gcccttttaa aatttctata acaagtctat catttccttc taaccaatac
     7561 cataatccta aacaaatcaa tatccaaaaa agaacataac cccaaaagta cttggtatag
     7621 attatctgat tcaacctcca catgtaacta cttaactttc ccatttccaa cttttacact
     7681 ctcctccagt ttctatgaac agaaaataca gtcaatggaa ttatgataga tagtaatgta
     7741 agccaataaa tcaatgcata cggattggaa tttaccatat actgaaaaat tggcaataat
     7801 ccaattatcc cattgtccaa ctgataaatc cagaataagg attctacaaa taaaaaagta
     7861 aacagaaata ctaagctcat gtttgatttt ttaaaccaag aaaaactgct aatagtaagt
     7921 aaggataaaa agaaatagta tgctatataa gagaaaaaaa atcctataaa gaaactagca
     7981 ttgtttgcaa tacttatacc ataaaattct cttaaaaaat caacctcctt tatctccaaa
     8041 gagaagctaa aaccattact aaatgcaatt agaaaaatca ataaaaataa agtcgccgtc
     8101 caaatccccg tactaagagc tgctaatttg aaactaaaac tggtaaagtg cttaattgat
     8161 ttcagacgaa tgcgacactc caacctatta aaatagttat tcatcaaata aaaaaagaac
     8221 aatatatatg tgaacggaaa gcaatatact ccagtcgtca tatcttgaag taaaactaag
     8281 atccattcta atacatttgg atggattgaa tattggcgac agcgcaataa atatactgta
     8341 ctagataaaa cacaggatag cagtaatata aaataaacca atactgctaa gaaatctttt
     8401 ttataaaatt gaacaaattg tttcattata cgtagtcctc tgaatgtaga aaaaatgtac
     8461 cataaacaac caaacaacta acaaataaaa taaaagcaag atgcccacta actaaggaaa
     8521 gactgatatc tttctgatat cccaaagcta atgttgtcac aggttctaag taagatagcc
     8581 ctaaaatagc ccaaaaaata ccaccaacca tcatataggc aactgggatg aaaatagctc
     8641 ctattttttt cttcactagc aaagcactag ctagtccaaa aatagagaac acagcgcccc
     8701 aaactccata ccagagagtc gtcacaagac tatagagcaa ctgattagaa tcaaataatt
     8761 cttttaaggc accactataa tctccaatat aaatttcctg ataaggagtc actaaaagat
     8821 taattcctaa taataataaa taggggagaa aaaagactag aaaagaagaa ataattgcag
     8881 tactacccac aatagctaga tacctctttt tagaaattcg gcacaattgt gctgttagaa
     8941 aatgactctc agcatcctct attatctgac tagaataggg cagtgtacag ataagtgcag
     9001 ctactaggct aatcggtgaa aatcctcgaa tagaagaagc ggcaaaaaaa atcgataaac
     9061 cttcaatttt ataaatacca ttgaaagcaa ggaaatttcc taaactcatg caaagaaggg
     9121 tcaaaaataa agacatataa aatcgaggtg attgaacgac tcgtacaaga ttacaaatga
     9181 aaaattccat ccttactcct ccttataata aaaatagggt gtagcattct tttttcatgc
     9241 tacacccaca atcaaccatc tttaaggctt actctgacaa gtaagttaat aagaatctgg
     9301 actccaagaa cctgaagtat gaattcttac atgatttcca aattgtggcg ccatagctaa
     9361 tctagtacca gaaccaatat aattgtcacc acctccatta tagtacatga caatcctaga
     9421 gccagacccc aatgaatata ccggggtaat atctgaccca ctataggcgc gctacgaata
     9481 gaggtattta accttttacc gccaccag
//
LOCUS       DAVKQB010000068         9178 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKQB010000068 DAVKQB010000000
VERSION     DAVKQB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.068"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            343..1386
                     /locus_tag="VW528_001896"
     CDS             343..1386
                     /locus_tag="VW528_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9912667.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIIARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1553..3640
                     /gene="topA"
                     /locus_tag="VW528_001897"
     CDS             1553..3640
                     /gene="topA"
                     /locus_tag="VW528_001897"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9912668.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEENIK"
     gene            3746..4105
                     /locus_tag="VW528_001898"
     CDS             3746..4105
                     /locus_tag="VW528_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9912669.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4198..4830
                     /locus_tag="VW528_001899"
     CDS             4198..4830
                     /locus_tag="VW528_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9912670.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGVLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQVGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            4874..5530
                     /locus_tag="VW528_001900"
     CDS             4874..5530
                     /locus_tag="VW528_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9912671.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5549..6169
                     /locus_tag="VW528_001901"
                     /pseudo
     CDS             5549..6169
                     /locus_tag="VW528_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     gene            6197..6631
                     /locus_tag="VW528_001902"
     CDS             6197..6631
                     /locus_tag="VW528_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU9912672.1"
                     /translation="MVAGSMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6643..7680
                     /gene="leuB"
                     /locus_tag="VW528_001903"
     CDS             6643..7680
                     /gene="leuB"
                     /locus_tag="VW528_001903"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU9912673.1"
                     /translation="MAKKIVALVGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAWPPLPDETLKASREADAILLATIGSPQYDGAVVRPEQGLMTLRKELNLYAN
                     IRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEE
                     VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFSDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSV
                     PDIAGQGIANPISMILSVSMMLRDSFGGYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7677..7946
                     /locus_tag="VW528_001904"
     CDS             7677..7946
                     /locus_tag="VW528_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9912674.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            7950..8309
                     /gene="leuD"
                     /locus_tag="VW528_001905"
     CDS             7950..8309
                     /gene="leuD"
                     /locus_tag="VW528_001905"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9912675.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8528..8671
                     /locus_tag="VW528_001906"
     CDS             8528..8671
                     /locus_tag="VW528_001906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912676.1"
                     /translation="MTSDKAGLERKFAAKEGKRNKPGVVLCGSMDELCALAQLNPKIE
                     AFY"
     gene            8690..8944
                     /locus_tag="VW528_001907"
     CDS             8690..8944
                     /locus_tag="VW528_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912677.1"
                     /translation="MLLGCILPWKPEAFEKLKAYGDGREELMTDVRGTSCFVIKFGKA
                     GEQLAAKLWEEGKMVYASYASMTKRLKLAMRARCNGVYGR"
     gene            8934..9095
                     /locus_tag="VW528_001908"
     CDS             8934..9095
                     /locus_tag="VW528_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912678.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNL
                     WDYRQVEYY"
BASE COUNT         2826 a         1624 c         2045 g         2683 t
ORIGIN      
        1 gtagagatta caaggcttgt ttaagaaaga attcaaagac cttgacaaat aaaaataaaa
       61 tggttattat aaaaaatggt ctgaaataga tgatgatact tttcgaaaat ctttttacat
      121 cagctcagct ttgccttgct gtgttttgag caagctacgg ttagcttccg agtttgattt
      181 tcatttacta gaaatgaaac tgatgagaga tatcagtaga catctgagtc aggatattat
      241 ggaaaatgat aaaaagagct cgtgagattg gcatatcaga ctactaaagt attgagtttg
      301 ttaggatttt agcgactagt tagctgggaa aggaagatat ttgtgacaaa taataaactg
      361 tattcgttga tagaatttag aaataaaata tatgaagaat tagaactttc cagaagtgat
      421 ttagcgattt tactatgtgc catgcttatc gcctctatcg gattaaatat ggattcgact
      481 cccgtgatta ttggagccat gttaatctct cctttgatga cacctattct gggagtgggg
      541 ctctctctag ctatatttga ttttaaattg ttaagaaaat cttttaaaat attagctatt
      601 caaattcttg ccagtctaat agcttcaaca ctttattttt atctttctcc catttcgtat
      661 gctagttcgg agattattgc tagaacctct ccgactattt gggatgttct cattgctttt
      721 gtaggaggga tagcaggtat tattggtgct aggaaaaaag agaccaataa tattgttcct
      781 ggtgttgcta ttgcaaccgc cttgatgcct cctctttgta cagtaggtta tgctattgct
      841 tctgctaatc taaaatttat cataggctcc tcttacctat tcctcatcaa ttgtagcttt
      901 attgtcattg cgacttatat aggtgttagg ttgatgatgg ttaagaaaca ttattttaaa
      961 gataatgaag aagactctaa aatgcgtagg attttgcttc tagttgctgt tttgctgatg
     1021 attccgagtt tcatctctgc aacgacttta gtgagagaaa cgttgaaaaa agagtccctt
     1081 aagaaattta tatcagagca gtttcagggg cataatattt tgaaaaaaac ctattctaaa
     1141 aagactcata ccctaaagct aaccatttca ggaaattatt tgacagaaga agaactcgat
     1201 atgatttcaa gtaagagagg tgactatggt ttaagtgatg tttctgttca agtttcacaa
     1261 ttgtctgatt cagaacaact tagtaaggaa gaactggtgg agtatttctt ccagtatatc
     1321 aaggataagg aagcaaaaga aaaggaaaaa gctaataagt tttatacaga gtctgaggag
     1381 caataatttc ttgagaatag ctggtttttc tcgtgagtct tctatgtata tcaaaggaag
     1441 actgaggttt taagtataaa acttttcttc tattatagta gctaagtctt gacagggttc
     1501 aaaaaatggt ttacacttta taaagtttat tactttgaaa aggtgtgata ctgtggctac
     1561 ggcaacaaaa aagaaaaaat caacagttaa aaaaaatcta gtcatcgtgg agtcgcctgc
     1621 taaggccaag acgattgaaa aatatctagg cagaaactac aaggttttag ccagtgtcgg
     1681 gcatatccgt gatttgaaga aatccagtat gtccgttgat attgaaaata attatgaacc
     1741 gcaatatatc aatatccgag gaaaaggccc tcttatcaat gacttgaaaa aagaagctaa
     1801 aaaagctaat aaagtttttc tcgcgagtga cccggaccgt gaaggagaag cgatttcttg
     1861 gcatttggcc catattctca acttggatga aaatgatgcc aaccgtgtgg tcttcaatga
     1921 aatcaccaag gatgcagtca aaaatgcttt taaagaacct cgtaagatcg atatggactt
     1981 ggtcgatgcc caacaagctc gtcggatctt ggatcgcttg gtagggtatt cgatttcgcc
     2041 tattttgtgg aagaaggtca agaagggctt gtcagcaggt cgcgttcagt ccattgccct
     2101 taaactcatc attgaccgtg aaaatgaaat caatgccttc cagccagaag aatactggac
     2161 agttgatgct gtctttaaaa agggaaccaa acaatttcat gcttccttct atggagtaga
     2221 tggtaaaaag atgaaactga ccagcaatga cgaagtcaag gaagtcttgt ctcgtctgac
     2281 tagtaaagac ttttcagtag atcaggtgga taagaaagag cgcaagcgca atgctccttt
     2341 accctatacc acttcatcta tgcagatgga tgctgccaat aaaatcaatt tccgtactcg
     2401 aaaaaccatg atggttgccc aacagctcta tgaaggaatt aatatcggtt ctggtgttca
     2461 aggtttgatt acctatatgc gtaccgattc gactcgtatc agtcctgtag cgcaaaatga
     2521 ggcggcaagc ttcattacgg atcgttttgg tagtaagtat tctaagcacg gtagcaaggt
     2581 caaaaacgca tcaggtgctc aggatgccca tgaggctatt cgtccgtcaa gtgtctttaa
     2641 tacaccagaa agcatcgcta agtatctgga caaggatcag ctcaagctat atacccttat
     2701 ctggaatcgt tttgtggcta gccagatgac agcggccgtt tttgatacca tggctgttaa
     2761 attgtctcaa aaaggggttc aatttgctgc caatggtagt caggttaagt ttgatggtta
     2821 tcttgccatt tataatgatt ctgacaagaa taagatgtta ccggacatgg ttgttggaga
     2881 tgtggtcaaa caggtcaata gcaaaccaga gcaacatttc acccaaccgc ctgcccgtta
     2941 ttctgaagca acactgatta aaaccttaga ggaaaatggg gttggacgtc catcaaccta
     3001 cgcgccaacc attgaaacca ttcagaaacg ttattatgtt cgcctggcag ccaaacgttt
     3061 tgaaccgaca gagttgggag aaattgtcaa taagctcatc gttgaatatt tcccagatat
     3121 cgtaaacgtg accttcacag ctgaaatgga aggtaaactg gatgatgtcg aagttggaaa
     3181 agagcagtgg cgacgggtca ttgatgcctt ttacaaacca ttctctaaag aagttgccaa
     3241 ggctgaagaa gaaatggaaa aaatccagat taaggatgaa ccagctggat ttgactgtga
     3301 agtgtgtggc agtccaatgg tcattaaact tggtcgtttt ggtaaattct acgcttgtag
     3361 caatttccca gattgccgtc atacccaagc aatcgtgaaa gagattggtg ttgagtgtcc
     3421 aagctgtcat cagggacaaa ttattgagcg aaaaaccaag cgtaatcgcc tcttctatgg
     3481 ttgcaatcgc tatccagaat gtgaatttac ctcttgggac aagcctgttg gtcgtgactg
     3541 tccaaaatgt ggcaacttcc tcatggagaa aaaagtccgt ggtggtggca agcaggttgt
     3601 ttgtagcaaa ggcgactacg aggaagaaaa tattaaataa gaggaggggc ccgaaagtaa
     3661 atttcaggct ccttttggtt agaacttgac aaaatacatc attttatgcg aaactagaag
     3721 aagattattt taactaggag aagttatgcg tcttatctat ctgataattg gttttttatc
     3781 actggccttg gctattgttg gggttgtttt acccttgttg ccgacaacgc ctttcctttt
     3841 gttgtctatt gcttgtttct ccagaagttc caagcgtttc gaagactggc tttatcatac
     3901 caagctctat caaacatatg tagctgattt tcgcgagact aagtctatta cgcgtgaacg
     3961 aaagaaaaaa atcatcgtct ctatctacgt cttgatggga atttctattt attttgcacc
     4021 tcttttacca gtcaaaatcg gtctgggtgc tttgaccatc tttatcacct attatctctt
     4081 caaggtcatt ccagacaaag aataattaaa acagtagtta tttcccttga taaaattgaa
     4141 agcatattca taacaatatg atataataaa tttgaagtaa tattcaagga gaatcaaatg
     4201 atttacgaat tttgtgctga aaatgtgacc ttgcttgaaa aagcgatgca ggctggagct
     4261 cgtcggattg aactctgtga taatctagca gttggtggga caacccccag ctatggagtg
     4321 actaaggcag cggttgaact ggcagctaac tacgatacaa ccatcatgac catgattcgg
     4381 ccacgtggtg gtgactttgt ctataatgac ctagaaattg ctatcatgct agaagacatt
     4441 cgtttgactg ctcaggctgg aagtcaaggg gttgtatttg gagttttaac tgctgataaa
     4501 aagttggata agcctaatct ggaaaagtta attgctgcat caaaaggaat ggaaattgtc
     4561 tttcacatgg cctttgatga actaagtgat gaagatcaac cggaagctat tgactggctc
     4621 agtcaagtcg gtgtcactcg tatcctaact cgtgctggtg tgtctggcga ctccttagaa
     4681 aaacgttttg ttcactatca cagaattttg gagtacgcta aaggtaaaat tgaaattcta
     4741 ccaggtgggg ggattgacct tgacaaccgt caaaccttta tcgaccaggt gggggtaaca
     4801 cagttgcatg gtactaaggt tgttttttaa aaaatagaaa ggaactgcta gctttgggta
     4861 gcagttttca cttatgtttg aaatttttaa atcctatcaa tttaatcaag aaaaggctca
     4921 tgattatggt tttatagaaa atagcgaagt ctggacatat agttgccaga ttttgcaagg
     4981 tgactttgtc atgactgtgt ccatcactgc tgataatgtg aactttcaag tctttgacca
     5041 agagactggc gacctctatc ctcacgttta tatggaaagc atgaggggaa gttttgtcgg
     5101 aaatgtccgt gaggcttgtc tggagattct ttaccagatt cggaaggctt gttttgatgt
     5161 gcaagatttt atctgtcatc agactaagcg tatcatgact caagttcagg aaaagtatgg
     5221 aaaccagttg gagtatctgt gggaaaaatc gcctgataca gctgtattgc gccatgaagg
     5281 caatcaaaag tggtatgccg tcttgatgaa aatctcttgg aataagctgg aaaagggcag
     5341 agaaggacaa gtggaagcag tcaacctcaa gcatgaccaa gtagctaatt tactttcaca
     5401 aaaggggatt tatccagcct ttcatatgag caagcgctac tggattagtg tgtcccttga
     5461 tgatacttta tcagatgaag aagtactgga attgatagaa aaaagttgga acttaacctc
     5521 taaaaaatga aatattttaa taattttcat gaactttcaa ttagctaaat attctttact
     5581 gaagaaattt tcagaaaata taggatttac cacactagag gaatatggtg catcttcaaa
     5641 tacctgattg agaatgtcaa gacggatcgt cagatcatct attcgcctca ctgtcatgat
     5701 gacctcggaa tggcagtggc aaatagcctt gctgctgtca agaatggtgc aggacgtgtt
     5761 gaagggacta tcaatggtat tagggagcga gctggaaatg ctgctttgga agaaattgca
     5821 gtggctctca atattcgcca agattactac caagtagaaa ccagtattgt cctaaatgag
     5881 accatcaata cgtcagaaat ggtttctcgc ttctctggta ttccagttcc taaaaacaaa
     5941 gccgtcgttg gtggcaatac cttctcccac gaatctggta ttcaccaaga tggagtcctt
     6001 aaaaatcctc tcacttatga gatcatcaca cctgaattgg ttggtgttaa gattccgctt
     6061 gggtcgccat gcttttgttg agaaactgag agaattggcc ctagatttta cagaagagga
     6121 tatcaaacca ctctttgcta agttcaaggc actggccgat aagaagtaag aaatcacaga
     6181 tgcagatatt cgagctttgg tagctggaag catggttgaa aatccagaag gcttccactt
     6241 tgatgattta caacttcaaa ctcatgcaga taatgacatt gaagcgctcg ttagcctagc
     6301 caatatggat ggtgagaaag tcgaatttaa tgcgacaggg caaggttccg ttgaagcaat
     6361 ctttaatgct atcgataagt tctttaacca atctgttcgt ttggtgtcct acactatcga
     6421 tgcggtaaca gatggaatcg atacccagga tcgggttttg gtcactgttg aaaacagaga
     6481 tacagaaacc atctttaatg cagcagggct tgattttgat gtgttgaagg cttctgctat
     6541 tgtctatata aacgctaata cctttgttca aaaagagaat gcaggtgaga tgggacgcag
     6601 tgtttcttac cacgatatgc ctagtgtgta aaggagaagg ctatggcaaa gaaaatagta
     6661 gctctagtag gagacggaat tggcccagaa atcatggagg ttggtttaga agttctggag
     6721 gctctagctg aaaaaacagg ttttgactat gagattgaca gacgaccgtt cggaggtgca
     6781 gatattgatg cagcatggcc tcccttacct gatgaaaccc ttaaggcaag tagggaagca
     6841 gatgctatcc tactagcaac tatcggtagt cctcagtatg atggagcagt ggttcgccct
     6901 gaacaaggcc tgatgactct ccgtaaggaa cttaatcttt acgctaatat tcgtcctgta
     6961 aaaatctttg acagtctcaa gcatttgtca ccactcaaac tggaacgaat tgctggtgta
     7021 gactttgtcg tggtgcgtga attgacaggc gggatttact ttggagatca tattcttgaa
     7081 gagcgcaatg cgcgtgatat caacgactat agctatgagg aagtggagcg gattattcgc
     7141 aaagcctttg aaattgcaag aaatcgcaga aaaatcctta ctagtatcga taagcaaaat
     7201 gttctagcga cctcaaaact ctggcggaaa gtagctgagg aagtcgcaca ggatttctca
     7261 gatgtaacct tggaacacca gctggtagac tcagctgcta tgcttatgat taccaatcct
     7321 gctaagtttg atgttattgt aacggagaat ctttttggag atattttatc tgatgaatca
     7381 agcgtcttat ctggtacact tggggttatg ccatcagcca gtcattctga aaatggacca
     7441 agtctctatg aacctattca cggttcagta cctgatattg caggtcaagg aattgccaat
     7501 cctatttcca tgattttatc agtttccatg atgttaagag atagtttcgg aggttatgag
     7561 gatgcagagc gtatcaaacg tgctgttgag acaagtttgg cggcaggaat tttaacgaga
     7621 gatataggag gtcaggcttc aacaaaggaa atgacggaag ctattattgc aaggttatga
     7681 agttagacga aaaaattact ctagtccttt tgatttggaa tgtcatcatt ttcttgattt
     7741 atggcattga caaattcaag gcaagaagaa gagcttggcg catcccagag aaaatcttac
     7801 tcattttagc ccttacttgt ggtggttttg gggcctggtt agctggaatc acttttcacc
     7861 acaagactcg aaaatggtac tttaaaacag tttggtttct tgggatggtg accacactag
     7921 tagccttata ttttatttgg aggtaatgga tggcagggtc ttcgagggaa cacgctgctt
     7981 gggctctagc ggactatggt tttaaggtcg tgattgcagg atctttcggt gacattcatt
     8041 acaataatga actcaataat ggcatgttgc caatcgttca gcctagagag gttagagaga
     8101 aactagccca gctaaaacca accgaccagg taactgtgga cttggaacaa caaaaaatca
     8161 tctcaccagt tgaagaattc accttcgaga tagatagcga gtggaaacat aaactcctaa
     8221 atagtttgga tgatatcggt attaccttgc agtatgaaga gttgattgct gcttatgaaa
     8281 aacaacgacc agcctactgg caggattaga aaaaatagaa aaggaaatat agtaaactga
     8341 aataagatgt aaacaaatga attggagctt aacatccatt tccagcaatt ttttagaaac
     8401 tacagtggac tattctggat tcaacacatt ataaaattat gacaaaacac attcacaaga
     8461 aggctacgac attttaaaag gtgagggcgg atgtatcgtt tgccctacta aagttggtta
     8521 cattatcatg accagcgaca aggcaggact tgagcgtaag tttgcagcca aagaaggtaa
     8581 gcgtaacaaa ccaggtgttg ttctctgcgg tagcatggat gaactttgcg ctttagcgca
     8641 actcaaccca aaaattgaag cattctacta aaaacattgg gatgaagata ttcttcttgg
     8701 ttgtatcctt ccttggaaac cagaagcctt tgaaaaactc aaagcatacg gggatggccg
     8761 tgaagaactt atgactgatg tacgtggtac tagctgtttt gttatcaagt ttggaaaagc
     8821 aggtgaacaa ttggctgcca agctttggga agaaggtaaa atggtctacg cctcatatgc
     8881 ttcaatgaca aaacgattga aactcgctat gagagcaagg tgtaatggtg tctatggtcg
     8941 ataaggacgg caaactcatc ccagaacaag gaggagcacg ttcaacctca ccagcaccag
     9001 ttgtgatccg taaagggctt gacattgata aaatcatgat gcacctgtca gacaccttta
     9061 acttatggga ctaccgtcag gttgagtatt attaggatag agaagaagtc tagtgttaag
     9121 gacattacag ctcataatac taggcttttt tatagaaatt cttttctttt tctatagg
//
LOCUS       DAVKQB010000069         9031 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKQB010000069 DAVKQB010000000
VERSION     DAVKQB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.069"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(298..1146)
                     /gene="dprA"
                     /locus_tag="VW528_001909"
     CDS             complement(298..1146)
                     /gene="dprA"
                     /locus_tag="VW528_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9912679.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDV
                     LAEFEF"
     gene            complement(1242..1931)
                     /locus_tag="VW528_001910"
     CDS             complement(1242..1931)
                     /locus_tag="VW528_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9912680.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1943..2821)
                     /locus_tag="VW528_001911"
     CDS             complement(1943..2821)
                     /locus_tag="VW528_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9912681.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2811..3680)
                     /gene="licA"
                     /locus_tag="VW528_001912"
     CDS             complement(2811..3680)
                     /gene="licA"
                     /locus_tag="VW528_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9912682.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3697..4719)
                     /locus_tag="VW528_001913"
     CDS             complement(3697..4719)
                     /locus_tag="VW528_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9912683.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4724..5431)
                     /locus_tag="VW528_001914"
     CDS             complement(4724..5431)
                     /locus_tag="VW528_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9912684.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGNRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5767..7254
                     /gene="tacF"
                     /locus_tag="VW528_001915"
     CDS             5767..7254
                     /gene="tacF"
                     /locus_tag="VW528_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9912685.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFIDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVVPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7264..8067
                     /locus_tag="VW528_001916"
     CDS             7264..8067
                     /locus_tag="VW528_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9912686.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8069..8878
                     /locus_tag="VW528_001917"
     CDS             8069..8878
                     /locus_tag="VW528_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9912687.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2838 a         1814 c         1527 g         2852 t
ORIGIN      
        1 aaagcgcaaa ctaggaagct agccacaggt tgctcaaagc actgctttga ggttgtagat
       61 aagactgacg aactcagctc aaaatactgt tttgaggttg cagatggaag ctgacgtagt
      121 ttgaagagat tttcgaagag tattatatga gaaaaatcct ctactctact aatccactta
      181 gaaaaaacgc atattagcaa gtgttaaaaa cttgctaata tgcgttttat tatggaaatg
      241 ttgggttcat tttctcttaa aattgatttt ttcttagaat tctagcttag gtcattttta
      301 aaattcaaat tccgcaagaa catcttgccc actggtgagc aattttgctc cttcttgaat
      361 caaatgatgg caaccgtctg atagtccatc taaaatgcta ccaggaatag caaagacatc
      421 gcgtccttct tccattgctc gctcacacgt aatgagacta cctgaacgca tcttagcctc
      481 tgctacaatc acaccacgac aaagtccagc aatgatgcga ttacgggcag gaaaatgaaa
      541 tttcagaggt tgttcgccag gtccatattc acttagaacc agatggtcat tgccgatgta
      601 gtcttgcaag cgtttattgg ctttaggata aaacacatcc agtcctgttc caatcactgc
      661 aatggttttt ccgccattct gaagagctgc catatgagct gctgtgtcaa tgcccttggc
      721 cagaccactg acaataacca gttcattttc caagccttga atgacttttt caactgactt
      781 agctccctgt ttgctacaag cacgactgcc cacgaccgct accttcggga atttcaggag
      841 gtcaagattt cccttgtaaa ataaaagtac aggcgcatca tatatttcac tcaaatccca
      901 aggataacag tcatctaaaa tagagaaaga tggaaatttt tgaaactctt tcgacaaatg
      961 cgcatcgtct atctgaaaat aacgttccat aaaaacggct ggattacggc aacctgatat
     1021 atctgcaata tcacccaaca aaagctcctg atcaacattt tcaccgtatt ctagcacttt
     1081 caaaatctgt tgattggtca aacctgattt ttttaactta tagatttcat agtttgtgat
     1141 tttcataaat aactccattt ctttttctac tcatctatct attcgtaaaa gttcgtaaaa
     1201 aatcaaaaaa catccgacta ttttgtcaga tgttggaatc tttaattttc gtttttaaga
     1261 atttcttcta atttacgata gtcttggaca ctatcgatct cataaatgct attgccttct
     1321 aattcttcaa catagacatc tagctctttg atattatcct taaccatatt gtcccaatag
     1381 agatcaacaa attcaccact tgcataagcc ttgtcgataa agctgacaat cttttctgca
     1441 gttggagcat cccagaagga tacaccacta aggatgcgac ctgccttgct atcaacaata
     1501 atgtcttgaa ccttgtagtc atctccatag accaagaacc attcgttggt acaatcttca
     1561 cgataaacac taaaataagt cgaacgtgtc aaatcattgc ggaacatatt tttaaagaga
     1621 taattgtcag catcaataac atagctgttg gccaattctt cttttacaag atagagagag
     1681 taaaagttat tgtagtcagc gtatttatca ttgaaaacga ggcgaacacc gtatttttct
     1741 tttaagtaat cgaattgttc tttaagataa ccaacaatga tgatgatgtc attgattcct
     1801 ttttctttga gaaactcaat ttggtattca atcaaaggtt tttgattaac ctgaaccaag
     1861 gctttagggg tattttcagt cataggacgc aagcgagttc ccaatcccgc tgctaagata
     1921 atggctttca cacgaatctc ctttattctt taataataat ataaactcca gcaatgacga
     1981 caagtgacgt cataatggtc agcatatcta gcggtgcacc caaaaaaaca actgcaaaca
     2041 agaccgtcca tactacatag ctcacgttca agcctgtagc cttggctggt tgcaagcgat
     2101 tgatagcgat ataataagcc aagtaggaaa tcatatcaaa ggctgcaaaa acaatcatga
     2161 gacctagcaa ttgtccattg gctactgcag taaatgactg atgagagaag agcacaatca
     2221 caagatagga caagaacgaa gtcacttgac ggattaagag ggcttcgatt tcactcagtt
     2281 cactttccat agcaaaggag ctaagaacac tctcacttcc ccatgcaata gcacaaacca
     2341 aagcacagag aatcccaatg tagaaggaat taacctgttc aaccttatag gtctgggcaa
     2401 taatccctcc aataatcaag acaatcccaa atacagtatt tttcgaaatc ttgtgcttca
     2461 aaaagaagaa agccaataga actgaaatcg cagggtaaat agccgataca gatgaagcta
     2521 aagaacttcc gatatactta actgcataaa gattggcctg cataccgata gggcctgcta
     2581 gcaaggctcc gatgataaca ctgacattgc gaatatttaa gaaaattgag aggcgaactt
     2641 tcccttcttt taccaagaga aaagctagta agataaagat gctcaaaaaa tcatgagctg
     2701 cagccaccac aaagggtgac aaatctgtaa aaatcgaaaa gatataagca ctaaccgtta
     2761 gacccaagcc ccagaaaata cctgagagaa ggccaaaagg aactccgttt ttacttttca
     2821 tctgaacctc cataagaagc caaaccttta acagctcttt ggtaacgatt cacaccatag
     2881 tcaccaaaat cttcaccttg ctcttcctta tagacagtcc atagactcca aatagtatct
     2941 tgtaaaattt tataaatagc aatcttttca tgagaaaccg gtgtttggtc actctcatag
     3001 tgagataaga aagtttcttc ctcttgggaa gtgaattcag actctaaaaa gagggcagcc
     3061 aaatcccaca ttggatcatt cattgatgaa tattcccagt caatcaaata aagtcgtcct
     3121 tgaggtgatt cgataaagtt ttcaggcacc aaatcgatat gacaagattt tctgtcaaca
     3181 cctaagtcag ccagtctttt ctctaaggag aagactgcat ttctaacaga ttcatagttg
     3241 gcataaggaa tttgttcttc aatcaaggat tcgtattttt tgatttcttc aaaaggagca
     3301 aattctcctc ttaattcctt agcagacgta tgaatagttt gtaatattgg agcaattttg
     3361 tcgaacttgg tcttgattga cgttgaatca agcgtaatcg cagattcgat atactcattt
     3421 actttgatac cagcttcaat atcaaaaaga taatttttta catctaagcc taaatccttt
     3481 agtagttcaa gattgtactt ttcatcttgt cgattgataa gcttttctgt ccctttacca
     3541 aagaatttaa caatgtattg cttatttgtt gttttggcca aatagttttg attggtcatt
     3601 ccacccagtt gttcaacact gaggacttcc tcttcttgac taagtaagga agaaattttt
     3661 tctttaatga ttttctccac aattaacctc cagtacttat acttcccact taaacactgt
     3721 tttaaaggct gtgtttaaat cggttgcaaa gacacgatga atatctttaa tttctcttac
     3781 aggttcttct agataaagga tatttttaag acgattggca aatttcttga cttccatcat
     3841 ttggatagca tttttaaaat caatgcgacc agaacgagat gacccaacca aaatcaagcc
     3901 cttttctaag gcatcgcgag tattgagatt gactttatat tcgctaactc ccatcatgag
     3961 aatcgttccc tgaggacgaa tgtagcgaat caagtcatta atagctggtc cagtaccatc
     4021 accaccacaa cattcaaaag catggtcaaa ggccaactct tcaggaatat tatccgtaat
     4081 atagcattct ttggcaaatg agaagagttc caacttttcc caatgacgac caataaccac
     4141 aatctctgat tctggcaaag tatagttgat aatattggca accacaaaag ctaaacttcc
     4201 atctccaata acggcgatcc gctcccgctt gctatgagca agagtcaata gacgattcat
     4261 agcgtgcatg cccacactga caaactctgt aatggctgca accgtatctt caatagcatc
     4321 ataagccacc acacgatctt tagggagaga aacaaactct ctcataaagc catcaaatcc
     4381 actagacaag aaatgggtcc ctgtcatgta gttttcatag aattcttcat cactctgcat
     4441 aggagactga ttgggaatca tgacaacttt ttgaccaacc tcgtaggttc cggtcgggtc
     4501 agaaatgacg gttccacatg actcgtgaat cattgccatt ggaagctttt tattcaaaat
     4561 cttgggatca cgttttccct gatagtaacg ctgatccgca tgacagacag ccatgtagtt
     4621 gggacggata aggatatgat tctcttggtc aatagcctct tcctgatatt tgacattgat
     4681 aaacttaggc ttagttagtt gataaatttg attaatcatt ttactagtct ttctcaatca
     4741 tactttttgc aatcttcaaa tctgttacgg ttgtaatctt cagatttgag tattcacctt
     4801 tggccaaggc cacatctttt cctttgatca caaagatttt acatgcatct gtcaagattt
     4861 ccttctcttc atcagaaaga gatccataaa ggtccatgaa gtccttgcaa cggaatgttt
     4921 gaggtgtttg tccttgataa aggtgagcac gatttggaat atctgtaata aattgaccat
     4981 tggtactttc aacgatagta tcaaccgctt ctaccactgt gtccactgcg tcatgatttt
     5041 gggcaagttg gatattgtcc tgaatcatgc gaagtgtaat aaatggacga acagaatcgt
     5101 gggtaacaac gatatcctct ggagtaagcg gacgataagc atcaatggct tcaatgattt
     5161 tcttaatact tgtattgcgg tcagcaccac cctttgtaat gatgatacgt tccttataaa
     5221 gaggaagata tttatctaca agatcttctg catgagaaac ccagtctcca tgaacaccaa
     5281 ctacaatttt ttcaatactt ggctccaaga caaatttttc aattgtatga atcaaaatag
     5341 gtcgattacc tagctctaaa aattgttttg gcaagttact gatccccatg cgtgtgccag
     5401 ttccaccggc aagaattcct gcataaatca tctattttct cctttgtctt actaaaaaaa
     5461 cttattctct ctattatacc acaatctagt cctatcataa aagaaatact ggtcctcccc
     5521 ttctagaata ggagaattat gcagttctat tattcaaaga aactagtcca tttctagtaa
     5581 tgactaggaa ttctgtagca attactaata aatggctgcg aaattttaga gtccttcaga
     5641 ataatatact ttccttaaag aaaacttaaa acttaagatt tgaaacttca aagtgattac
     5701 ttgacttact gcccttcatt tcttattagc taactttatg atataatgaa aaacgaggta
     5761 aattgaatga aaagtataaa attaaatgct ctatcttaca tgggaattcg tgtcttgaat
     5821 attatttttc ccatcctaac tggaacctat gtcgcgcgtg tcttggaccg aactgactat
     5881 ggttacttca actcagtcga cactattttg tcatttttct tgccctttgc aacttatggt
     5941 gtctataact acggtttaag ggctatcagt aatgtcaagg ataacaaaaa agatcttaac
     6001 agaacctttt ctagtctttt ttatttgtgc atcgcttgta cgattttgac cactgctgtc
     6061 tatatcctag cctatcctct cttctttatt gataatccaa tcgtcaaaaa ggtctacctt
     6121 gttatgggga ttcaactcat tgcccagatt ttttcaatcg aatgggtcaa tgaagctctg
     6181 gaaaattaca gttttctctt ttacaaaact gccttcatcc gtatcctgat gctggtctct
     6241 attttcttat ttgttaaaaa tgaacacgat attgttgtct atacacttgt gatgagttta
     6301 tcgacgctga ttaactacct gattagttat ttttggatta aaagagacat caaacttgtt
     6361 aagattcacc taagtgattt taaaccactc tttctccctc tgacagccat gttagtcttt
     6421 gccaatgcca atatgctctt cactttttta gatcgcctct tcctcgttaa aacagggatt
     6481 gatgtcaacg ttagttacta taccatagct cagcgaattg tgaccgttat agctggggtt
     6541 gtaacaggtg caattggagt gagtgtgcct cgtctcagtt actatctggg gaaaggagac
     6601 aaagaagcct atgtttctct ggttaataga ggtagtcgaa tctttaactt ctttatcatt
     6661 ccactgagtt ttggactcat ggttttagga ccaaatgcca tcctacttta cggtagtgaa
     6721 aaatatatcg gaggcggcat cttgacctct ctcttcgctt ttcgtacgat tatcctggcc
     6781 ttagatacca ttcttggttc ccaaattctc tttaccaatg gctatgaaaa acgtatcaca
     6841 gtctatacag tctttgctgg gctactcaat ttgggcttga atagtctcct ctttttcaac
     6901 catatcgtgg ttcctgaata ctacttactg acaactatgt tatcagagac ttctctactt
     6961 gttttctata ttattttcat ccatagaaaa caactcatcc acttgggaca tatctttagc
     7021 tatactgttc gatactctct cttttcactt tcctttgtag caatttattt cctgattaat
     7081 ttcgtgtatc ctgtggatat ggtcattaat ttgccatttt taattaatac tggtttgatt
     7141 gtcttgctat cagctatctc ttatattagt ctacttgtct tcacaaaaga tagcattttc
     7201 tatgaatttt taaaccatgt cctagcctta aaaaataaat tcaaaaaatc ataggagttt
     7261 aaaatgaaac aactaaccat tgaagatgcc aaacaaattg aattagaaat tttggattat
     7321 attgatactc tctgtaaaaa gcacaatatc aactatatta ttaactacgg tactctgatt
     7381 ggggcggttc gacatgaggg ctttatccct tgggacgacg atattgattt atccatgcct
     7441 agagaagact accaacgatt tattaacatt tttcaaaagg aaaaaagcaa gtataagctc
     7501 ctatccttag aaactgataa gaactacttt aacaacttta tcaagataac cgacagtacg
     7561 actaaaatta ttgatactcg aaatacaaaa acctatgagt ctggtatctt tatcgatatt
     7621 ttccctatag atcgctttga tgatcctaag gtcattgata cttgttataa actggaaagc
     7681 ttcaaactgc tgtctttcag taaacataaa aatattgtct ataaggatag ccttttaaaa
     7741 gattggatac gaacagcctt ttggttgctc cttcgaccgg tttctcctcg ttattttgca
     7801 aataaaatcg agaaagaaat tcaaaaatat agtcgtgaaa atgggcagta tatggctttt
     7861 attccttcaa aatttaagga aaaggaagtc ttcccaagtg gtacctttga taaaacaatc
     7921 gatttaccct ttgagaattt aagccttcct gcacctgaaa aatttgatac tattttgaca
     7981 caattttatg gagattatat gaccctacca ccagaagaaa aacgcttcta cagtcatgaa
     8041 tttcacgctt ataaattgga ggattaggat gcaatattta gaaaaaaaag aaattaaaga
     8101 aattcaacta gccctgctgg actatattga tgagacttgt aagaaacatg atattcctta
     8161 ttttctcagt tatggaacca tgcttggagc catccgccac aaaggtatga ttccttggga
     8221 tgatgatatt gatatttccc tttatcgtga ggattatgag cgtttactga agattattga
     8281 agaagaaaat caccctcgct acaaggttct ttcctacgat acatcttctt ggtacttcca
     8341 taatttcgca tcgattttgg acacttctac tgttatagaa gaccatgtta agtacaagcg
     8401 tcatgatacc agccttttca tcgatgtctt cccaattgat cgatttacag acttgagcat
     8461 tgtcgacaag agctataagt atgtggccct tcgtcaacta gcttatatca aaaaatcacg
     8521 agcagttcac ggtgatagca aactaaaaga ttttcttaga ttatgtagct ggtacgctct
     8581 ccgatttgtc aatcctcgct acttttacaa gaaaattgat caactagtca aaaatgctgt
     8641 aaccaacact cctcaatatg aaggaggagt tgggatcggt aaggaaggga tgaaagaaat
     8701 cttcccagtt gataccttta aagaactgat tttaactgag tttgagggcc gtatgttgcc
     8761 tgttcccaaa aaatatgacc aatttttaac ccagatgtat ggcgattata tgacaccacc
     8821 atcaaaagaa atgcaagagt ggtatagtca tagcattaaa gcttatcgca aaaactgatt
     8881 gagggggatt atacaaacta ctaagataga ggttattcaa aaacataatt ttagtagaaa
     8941 atgaaatata tattcccaca ataaaacgca tcatatcaag gtttttgaaa aaccttgata
     9001 tgatgcgttt tataatttta aagacttttt t
//
LOCUS       DAVKQB010000070         8963 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKQB010000070 DAVKQB010000000
VERSION     DAVKQB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.070"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            227..2014
                     /locus_tag="VW528_001918"
     CDS             227..2014
                     /locus_tag="VW528_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9912688.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2011..2487
                     /locus_tag="VW528_001919"
     CDS             2011..2487
                     /locus_tag="VW528_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9912689.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2515..3420
                     /locus_tag="VW528_001920"
     CDS             2515..3420
                     /locus_tag="VW528_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9912690.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3407..4222
                     /locus_tag="VW528_001921"
     CDS             3407..4222
                     /locus_tag="VW528_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9912691.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4229..4633
                     /locus_tag="VW528_001922"
     CDS             4229..4633
                     /locus_tag="VW528_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9912692.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4886..6052
                     /locus_tag="VW528_001923"
     CDS             4886..6052
                     /locus_tag="VW528_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9912693.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCATDGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6168..7205
                     /locus_tag="VW528_001924"
     CDS             6168..7205
                     /locus_tag="VW528_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9912694.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7413..8267
                     /locus_tag="VW528_001925"
     CDS             7413..8267
                     /locus_tag="VW528_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9912695.1"
                     /translation="MTCEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWNIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            8417..8674
                     /locus_tag="VW528_001926"
     CDS             8417..8674
                     /locus_tag="VW528_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU9912696.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
BASE COUNT         2559 a         1681 c         2029 g         2694 t
ORIGIN      
        1 atattttata aatgtattct atacaaaatt attttaggtc ctatattccg aatttaaaga
       61 gcgtttgaaa tgggtctaaa aatgtttgaa actaattgaa tgtgtgtata atagtaaaaa
      121 tgcagtaaat atcccgacaa tttctattga caattcaaac agattggttt ataattaata
      181 taacaacaaa tgaaagcgca aactttcgcg gtcggaaggt agttttatga cacgatttga
      241 gatacgagat gatttctatc tcgatggaaa atcatttaag attttatctg gtgccattca
      301 ttattttagg gttcctccag aggattggta tcattcgctc tataacttga aggctcttgg
      361 ttttaatacg gtagagactt atgttgcttg gaatttacac gagcctcgtg aaggtgagtt
      421 tcattttgac ggtgatctgg atttagagaa atttctccaa atagcgcagg atttgggtct
      481 ctacgcaatt gtgcgtccgt ctccatttat ctgtgcggaa tgggaattcg gtggcttacc
      541 agcttggctc ttgaccaaga acatgcgaat tcgctcatcc gacccagcat atatcgaggc
      601 agttggtcgc tactatgatc agttattgcc aagactggtt cctcgtttgt tggacaatgg
      661 tggcaatatt ctcatgatgc aggttgaaaa tgagtatggt tcttacggag aagataaggc
      721 ttacctgaga gcgattcgac agctaatgga agagtgtggc gtaacctgtc ccctctttac
      781 atcagatggt ccatggcgag ctactctgaa agctggaacc ttaattgaag aggacctctt
      841 tgtaacagga aactttggtt ctaaggcacc ttacaacttt tcgcagatgc aggaattctt
      901 tgatgaacat ggtaagaaat ggccactcat gtgtatggag ttctgggatg gttggtttaa
      961 tcgttggaaa gaaccgatta tcacacggga tcctaaggaa ttggcagatg cagttcgaga
     1021 agttttggaa caaggctcca tcaatcttta catgttccac ggtggtacaa actttggttt
     1081 catgaatggt tgctcggctc gaggaacttt ggacctgcca caagttacgt cttatgatta
     1141 cgatgccctt ctggatgaag aaggaaatcc aactgctaaa tatcttgcag tcaagaagat
     1201 gatggcaaca catttttcag agtatccgca gttggaacca ctctacaaag agagtatgga
     1261 gttggatgct attccactag ttgaaaaagt ttctttgttt gaaaccttag atagcttgtc
     1321 aagtcctgta gaaagtctct atcctcaaaa gatggaggag ctgggacaaa gttatggcta
     1381 cctactttat cgaacagaaa caaactggga tgcagaagaa gaaagacttc gtatcattga
     1441 tggtcgagat agggcccagc tgtatgtcga tggtcagtgg gttaaaactc aatatcagac
     1501 agagattggg gaagatattt tttatcaagg taaaaagaaa gggctatcta ggttagatat
     1561 cttgatagaa aatatggggc gtgtcaacta tgggcataag ttcttagcgg atacgcaacg
     1621 taagggaatt cggacagggg tctgtaagga tctgcatttc ttactaaact ggaaacacta
     1681 tccactccca ctagacaatc ctgagaaaat tgatttttca aaaggatgga ctcaaggaca
     1741 accagccttt tacgcttatg actttacagt cgaagagcca aaagatactt acctagactt
     1801 gtctgagttt ggtaaggggg ttgcctttgt caatgggcag aatctaggac gtttttggaa
     1861 cgttggccca actctctcac tttatatccc tcatagctat ctcaaggaag gtgccaaccg
     1921 catcattatc tttgaaacag aaggtcaata taaagaagag attcatttaa ctcgtaaacc
     1981 tacactaaaa catataaagg gggaaaactt atgacaattg taggatgccg tattgatgga
     2041 cgtttgatcc acggacaagt agccaatctt tgggctggaa aactaaatgt ttcacgcatt
     2101 atggttgtag acgacgaagt tgtcaacaac gatattgaaa agagtggttt gaaacttgcg
     2161 acaccaccag gtgtgaaatt gagtattttg ccagttgaga aagctgcagc caatattctt
     2221 gctggcaaat acgatagcca acgtctcttt atcgtggctc gtaaaccaga ccgcttcctt
     2281 ggtttggtag aagcaggtgt accacttgaa acccttaatg ttgggaatat gtctcaaaca
     2341 ccagaaactc gttctattac acgttctatc aacgtagtag acaaggatgt ggaagacttc
     2401 cacaaactgg cagaaaaagg tgttaaactt actgctcaga tggttccaaa tgatccaatt
     2461 tcagactttt tgagcttatt aaaataggaa aaaatttttt aggaggtcat tgttatgata
     2521 caatggtggc aaattttact tctcactttg tactcagctt atcaaatctg tgatgagttg
     2581 acgatcgttt catctgcagg ttcccctgta tttgctggtt tcattactgg tttaatcatg
     2641 ggagatgtga ctactggttt acttatcggt ggtaacttgc aactgttcgt tcttggggtt
     2701 ggtaccttcg gtggtgcttc tcgtatcgac gcaacttctg gtgcggttct tgcgacagcc
     2761 ttctctgttt cacaaggaat tgatgcaccg cttgcaatta ctacaatcgc tgtaccagta
     2821 gcagctctct tgacttactt cgacgttctt ggtcgtatga ctactacctt cttcgctcac
     2881 cgtgtggatg ctgcaatcga acgctttgac tataaaggta ttgaacgcaa ctacttgctt
     2941 ggtgcgattc cgtgggctct atctcgtgcc cttccagtct tctttgccct tgcttttggt
     3001 ggtgcctttg tacaatcagt agtagacttc gttgaagcct acaaatgggt tgcagatggc
     3061 ttgacactcg caggacgtat gcttccaggt cttggatttg caatcttgct tcgttacctt
     3121 ccagttaaac gtaaccttca ctaccttgct atgggatttg gtttgacagc tatgttgact
     3181 gttctttact catatgtaac aggtcttggt ggcgctgttg ctggtatcgt aggtactctt
     3241 cctgctgaag ttgctgaaaa aattggtttc gtgaacaact tcaaaggttt gtctatgatt
     3301 ggtatttcta tcgtaggtat tttccttgca gtgcttcact tcaaaaatag ccaaaaagta
     3361 gctgtagcag caccttctac accatcagaa agtggggaaa tcgaagatga cgaattctaa
     3421 ttacaaactt acaaaagaag attttaatca aatcaacaaa cgtagcttgt ttactttcca
     3481 attaggttgg aactacgaac gtatgcaagc ttctggttac ctttacatga tcttgcctca
     3541 gttgcgtaaa atgtatggtg atggaactcc tgaattgaaa gaaatgatga aagttcatac
     3601 tcaattcttc aatacttcac cattcttcca taccattatc gctggttttg accttgccat
     3661 ggaagaaaaa gatggtgtag gttcaaaaga cgctgttaac ggtatcaaga caggtttgat
     3721 gggaccattc gctcctcttg gggatacaat ctttggttca cttgtacctg ctatcatggg
     3781 gtcagtcgca gcaactatgg ctatcgctgg ccaaccttgg gggatcttcc tttggattgc
     3841 agttgcagta gcgtatgaca tcttccgttg gaaacagttg gaatttgctt acaaagaagg
     3901 ggttaacctt atcaacaaca tgcaaagtac cttgacagct ttgattgacg ctgcatctgt
     3961 acttggtgtc ttcatgatgg gtgctcttgt agcaacagtg attaactttg aaatttctta
     4021 caagttgcca atcggtgaaa agatgattga tttccaagac atcttgaacc aaatcttccc
     4081 acgtttgctt ccagcaatct ttactgcctt tatcttctgg ttgcttggta aaaaaggtat
     4141 gaactctact aaagctatcg gtattattat cgtacttgct ttggctcttt ctgcccttgg
     4201 tcactttgca cttggaatgt aattccttat gactaaatca ttaattttgg tgagccatgg
     4261 tcgcttctgt gaggagctta gaggtagcac agaaatgatt atgggcccac aagacaacat
     4321 ttacacagta gctcttcttc cagaagatgg cccagaagaa tttactgcta aatttgaagc
     4381 tgttattgaa ggattggatg atttcctagt ctttgcggat cttctcggtg ggacaccttg
     4441 taatgtggtg agtcgcttga tcatggaagg tcgtgatatt gacctttacg cagggatgaa
     4501 tcttccaatg gtgattgaat ttatcaatgc gagccttaca ggcgcagatg cggactacaa
     4561 gagccgtgct gcagaaagca ttgtgaaagt taatgacctg ttagcgggct tcgatgatga
     4621 cgaagatgaa taatactctt cgaaaatctc ttcaaactac gtcaacgtcg ccttgccgta
     4681 ggtatatgtt actgacttcg tcagtcttat ccggcaacct caaagcggtg ctttgagcag
     4741 cctgcggcta gtttcctaca gattttagtt ggaactcgat tcaattcatg tgacaacgtg
     4801 aaaatcgtta gagcatttta tatagaatat acatgggaat ggagcttact cccattccca
     4861 tatttaatag aaaaagagga actcaatgct acattataca aaagaagact tgctcgaatt
     4921 gggtgcagaa atcactacgc gtgaaatcta ccaacagcct gatgtatgga gagaagcttt
     4981 tgaattttat caagcaaaac gtgaagaaat tgcagccttt ctacaagaaa tcgctgataa
     5041 acatgactat attaaggtta tcttgacagg tgctgggact tctgcttatg tgggagatac
     5101 cttgctacct tactttaagg aagtctatga cgaacgcaaa tggaatttca atgctattgc
     5161 gacaacagat atcgttgcca aaccagcaac ctatttgaaa aaagatgtgg caactgtcct
     5221 tgtgtctttt gctcgtagtg ggaactcgcc tgaaagtgtg gcgactgttg atttggccaa
     5281 atccttggtg gatgagcttt atcaagtgac gattacttgt gcaacagatg gtaaattggc
     5341 tcttcaagct cacggtgatg accgtaatct cttgctcttg caaccagctg tctctaatga
     5401 tgctggattt gccatgactt ctagctttac gtctatgatg ttgacagctc tcttggtctt
     5461 tgatcctaca gaatttgctg ttaagtctga acgttttgaa gttgtatcta gtcttgcccg
     5521 taaagtttta gacaaggtag aagatgtcaa agagctcgtt gatttagact ttaaccgtgt
     5581 catctatcta ggcgctggtc ctttctttgg acttgctcat gaagctcagc tcaagatttt
     5641 ggaattaact gctggtcaag ttgcgaccat gtatgaaagc ccagttggct tccgtcacgg
     5701 tccaaaatct cttatcaacg acaatacagt tgttttggtc tttggtacaa cgacagccta
     5761 cactcgtaag tacgacttgg acttggttcg tgaagttgct ggtgaccaga ttgctcgtcg
     5821 tgttgtgctt ttgagtgatc aagcttttgg tcttgaaaat gtcaaagaag tggtccttgg
     5881 ttgtggcggt gtcttgaatg atatttaccg tgtcttccct tacatcgttt atgcccaact
     5941 ctttgctcta ttgacttcac tcaaggtaga aaataaacca gatacaccgt ctcctacagg
     6001 tacagtaaac cgtgtagtac aaggtgtcat aattcacgaa tatcaaaagt aagacagtgt
     6061 ttatgaattc ttgacaagag gatttgtaaa ttatcagata aaccatagat tgtcagtacg
     6121 ctttctatgg tttgtttgct tgagagaaat agtaaaagga gaacagaatg aaagcataca
     6181 cagagcgtgt atttggaaat gttgagggcg aggatgtctt ggcctatcga tttgagacag
     6241 acggtggcta ccaacttgag gttatgactt atggcgcgac catcttgcgc tatgtcgcac
     6301 ctgacaaggc tggaaatttt gccaatgtta tcttgggatt tgatgacttt gatagttatg
     6361 taggcaatag tcccaagcat ggagcaagtg taggtcctgt agcgggtcgt attgcaggtg
     6421 cgacctttga gctcaatggt aagacctatg accttgaggt taataatgct agcaactgta
     6481 atcacagtgg ttcaactggt tgggattcca gcttgtttga agttgaagaa gtaagcgatc
     6541 atggcttgac tctctacaca gagcgtacag atgggacagg agggttccct ggaaatctca
     6601 agatttggat cagttatcac ttggaagaaa ctggtgccta tgaaatcatc tacaaggtaa
     6661 cgaccgatca ggatacgctg gtcaatccaa ccaaccacag ctatttcaac ttgtctggtg
     6721 atttcacgca gacgattgac cgtcatgtct tccaactaaa cacagagggc atttactcaa
     6781 tcgctcctga cggtgttcct gccaaaactc cagaagccaa ccgtgatgtg gtcaaacaca
     6841 tctacaatgg taccttgttg aaggatatct ttgcagaaga agatgagcaa atccagctgg
     6901 catcaggttt ggatcaccca tttgcccttc ctgcaggcca tgacaatgct ggattccttt
     6961 atgaccaaaa ttcaggtcgc ttcctgcttt tcaagacaga agctccttgc tttgtggtct
     7021 acacagcaaa ctttgtggat gaaagtgtca tcataggagg tcagccaatg ctacagcaca
     7081 atgggattgc tcttgaagcg caagctttac cagatgccat tcacagtgac cttaaaggcc
     7141 aagtcattct taaagctggt caaaccttca ccagtaagac acgttatgaa cttgttgtga
     7201 aataaaaaaa gctggtcaaa aagtctttaa aattaaaata acgcatattg tcaggtgatg
     7261 aaaagtttga taatatgcgt tttattatga aatgatttac tttatcttat cctgaaatgg
     7321 tgttattttt gagttatctg gcttgacttt cttgatggaa attatataat agttgtaata
     7381 acaataatta caacgagaaa agaggaccat ttatgacctg tgaatacaag agtcacattt
     7441 atttggcaga gacagtttta aatgtaaagg atttggccag tcaaacagcc ttttatcagc
     7501 aagttattgg tttagaaatc ttatctcaaa ctgagacaga gtccattcta ggccttggtg
     7561 gaaaagtctt ggtacagctg attcaagcac aagagagcgg agaagtgagg gaacattatg
     7621 gtctttacca tttggccatt ctcttgccca cacgcaaggc tttggctgat gtcttgaaac
     7681 acctgacaga tttgcaaatt cctcttgtcg gcggtgcaga ccatggttac agtgaagcta
     7741 tttacttgga agatttggag ggaaatggca ttgaactcta tcgagataag ccagtttcca
     7801 catggaatat tcgagaagat ggacgcatta tcggagtgac ggaggctctt gcggcgcagg
     7861 acatttatga gttgggggaa agagtagagc cctttatcct ggcagaaggt acaagaatgg
     7921 ggcatattca tctttccgtc aaggatagtc gaaagtccag ccagttttat caaaaggtgt
     7981 tagggctaga gaataaattc agtgtgcctg gcgctagttg gattgcggct ggagattacc
     8041 atcatcattt agcagttaac gaatggggag gaaaaggtct ggcttcgcgt aagcaaggct
     8101 taccaggttt agcctactat gtcatcgaag tcgcacataa aaaagaactg ttaacgattg
     8161 cccaacgagc acaagaagtt gacgcaccaa tcaaatggat gacatcgagc caattggaaa
     8221 tcacagactc agatggcatc gtgacccgta ttcgtttagc aaggtaaaat taggaatttt
     8281 taataagtta taagtattgc tagtttgctt tttgaacggt ctttggtaac agaactgcgt
     8341 gagctggatg ggtgagaatt ttgacaaaga agctgattag agatataatg aagaaaaatc
     8401 gagcaaggaa aaagaaatga caaattcaaa gtatattact tgtttgaaac gttcagaggg
     8461 ccagttgtgt ggaattcaaa agatgattga aggagatcgt gactgtgctg atattgtgac
     8521 gcaactgaca gcagtgaaat ctagtgtaga gcgcgtgatt gagatgataa ttaccgaaaa
     8581 tcttactgaa tgtatcaatc agccactaga tgattctgaa gctcaaaagg aacgcctaga
     8641 aaaggctatc cgatacttga ttaaacggaa ataaatgaat gttttgagac gatatgtgca
     8701 aaggtctatt aactattaga atagtttaat gtgagtcaaa agaagttgta aaaaatcttt
     8761 aaaattaaaa aacgcatagt atcaggtatt gaaatgtact gccccccaaa agttagattc
     8821 tttctgtcta acttttgggg ggcagtacat aaaaaccttg atagtatgcg ttttattgtg
     8881 gggagactta tactcttcga aaatctcttc aaaccacgtc agtttcacct tgccgtagat
     8941 atggttactg acttcgtcag ttc
//
LOCUS       DAVKQB010000071         8283 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKQB010000071 DAVKQB010000000
VERSION     DAVKQB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.071"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            146..793
                     /locus_tag="VW528_001927"
     CDS             146..793
                     /locus_tag="VW528_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU9912697.1"
                     /translation="MVVGIAIASGYLNSHLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            924..1337
                     /locus_tag="VW528_001928"
     CDS             924..1337
                     /locus_tag="VW528_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912698.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            1356..1541
                     /locus_tag="VW528_001929"
     CDS             1356..1541
                     /locus_tag="VW528_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912699.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(1808..2251)
                     /locus_tag="VW528_001930"
     CDS             complement(1808..2251)
                     /locus_tag="VW528_001930"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9912700.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(2281..4503)
                     /locus_tag="VW528_001931"
     CDS             complement(2281..4503)
                     /locus_tag="VW528_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9912701.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYPSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5519..6148
                     /locus_tag="VW528_001932"
     CDS             5519..6148
                     /locus_tag="VW528_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9912702.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(6271..8163)
                     /gene="pepO"
                     /locus_tag="VW528_001933"
     CDS             complement(6271..8163)
                     /gene="pepO"
                     /locus_tag="VW528_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9912703.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         2248 a         1902 c         1590 g         2543 t
ORIGIN      
        1 ttattttcag ggttttattt ctatcatttt attttacttt catttggtgg gagaatattt
       61 gaaaaaaaga aaggtagttt aacctatgaa aaaatcacta aaaatttttg ctacatctaa
      121 atagtttgac ctcttcgggg ttgctttggt cgttgggatt gcgattgcat ctggttacct
      181 caactcacac ctcgataaat ttgtagactg gggatcatgg acagctcttg taccctttgg
      241 attgatttcc gtaaccaacg ttgggatttc catgttgtcc actcgtttca cggggaaatt
      301 aagcaaatgg ggaaattact ttggtattgt taataccatt ttgtccggtg ctattgatta
      361 tatccttgga aataaggcgg ccatcattac ctatcccgtc accttcctca tttatacctt
      421 tgctataaag aagtgggaag cttcgcaaga aggcagaccc aaccaaatga gccaaaaaca
      481 ggtaaaattg gcggccatca tcatttccat catcgccttc ctctttgcct ttgtgaccaa
      541 ctatatcgga tatgggggca agatgaatct ccttgcctac gtaacaacta ttgcctttgc
      601 actgtccctc attgccaatg ctttgaatgc attaaaacta acaactcagt ggggcttttg
      661 gttgatttac aatttcgttc agctgacaaa agctggtatt caaggaaact tcgccaatat
      721 cggtaaatac atcttttata tcctcaatgc aatcggagct ttatttgtct ggaatgatga
      781 agaagttaga taatagaagg aaactcaaat aaagaatact agccaactta agagaatctc
      841 ccttctgcta tttttatcat tttagttgta actcccattc ttttggagta gaatgaagtt
      901 agataaaaga gggaggtctt gtcatgaaaa aactctatag aatccatttt atagcaattg
      961 cagttattga tttgctacta tttgcatttt ttatcactag actcgagaca tcttttgagt
     1021 ggctcttgct ctctggttta attttcttcc ttgctcaagg actcctgcta tttctcttgg
     1081 tcgtccgact caaacatcaa ttcgctgaga tttatcctca aatcaataaa aagattcgct
     1141 tctactattt aggggttctc accattgatt ttctattttt tgttctctta gccttcatta
     1201 gttctcagcg tttttcatct cttatgccaa tcatcactgc ttgccattct actttttatt
     1261 atatgacagc tgactaccta agagaaaact atccagactt ttacgacaaa cacatctctt
     1321 tatgggagtg tctctaagga aaaggaggtt ttagcatgaa aaaaatcatc ttcatcaaaa
     1381 ccattcaact ccttgtcatt gatggaatca tgctggcatt tttgacattt aaaagggggc
     1441 ttacttggga ctggattttg atttatagcg gttggctcat tttctttcat cctgtgctat
     1501 tgacctatct ttcaaaccaa ctttgtgacc actttagtta actctattcc cagattagac
     1561 cgagattctg gcgttttgct ttacaaattc tcctatggga tagcctgatg attctctcct
     1621 tggtgtcttt aagtgatatt ccacttttcc ttcagggaac tctcctcatc ctaggacatc
     1681 tcatcccttc ctatcgcatc tgccaaagcc tgaaaagaga cttcccccaa gcatatcaag
     1741 aaccgatttc tttttggagt attttatgat agatgagaaa gaccaagccg actgggcttg
     1801 gtctttctta tctcttttta gtatctagga taatggtaac aggtccatta ttaaccagct
     1861 caacctgcat atctgctcca aagatgcctg tctgaacggg cacttcttgc gctaattttt
     1921 gattgaaagc atcatagaag tctgatgcca tatcaggttt agctgcccct gtaaaggctg
     1981 gacgattgcc tctcttagta tccgcaaaga gggtaaactg agaaatagag aggatttctc
     2041 cttcaatatc tttgacagac aggttcatct tgccttctgc gtctgaaaaa atccgcatat
     2101 tgaccagttt tctcacagca tagtccaaat cttcctcttg gtcctctggt ccaacaccaa
     2161 ccagcaataa aagtccctga ttgatttttc cctgaatctg gccttctata ctcacttggg
     2221 cttttttaac ccgttggata atgattttca taatagcctt tctagtaaga gctaggacaa
     2281 ctagccgttg gtccgtttga cagagtaaac ttctggcaca ctcttaattt tatcgacaac
     2341 cgtggtcagt gtagagaggt tggcaatacc gaaggacaca tggatattag caaacttcat
     2401 atccttggtt ggttgggcat tgaccgttga aatattcttg gttgtatttg aaagaacttg
     2461 cagtacatcg ttcaacagtc ctgtacggtt gagaccgtag atatcgatat gggccatata
     2521 ctccttattt gagctagggt actggtcttc ccattccaca tcaaggagac gttgctcgta
     2581 gttttcttgg gcacgcaggt tcatacagtc cacacggtga atagccacac cacgaccctt
     2641 ggtaatgtag ccaacaatat cgtcaccagg cacggggtta caacacttag caatccgcac
     2701 taggagacca gaagcacctt caataaccac tcccccctca tgcttgacct tgagggtttc
     2761 tttattttca accttgacct cgccaccttt gacaagctcc tctgcctcag ctttggcctt
     2821 ggcacgctct tcctcacggc gttccttttc agtcagacgg ttaaagacgg taatcgcacc
     2881 gatttcccca aaaccaatgg ccgcaaagag ggagtcttct gtcttgtaac tggtcttttg
     2941 cagaacttga tccatgtggc gcttgtccat aaatttattt gccacatagc cattttcttg
     3001 gaactgagcc atcagcatct cacgaccctt gttgacagac aattccttat cttggttttt
     3061 aaagaactgg cgaatcttat tgcgcgcctt gctagtcttg accatattga gccagtcacg
     3121 gctaggtcca aaggagttcg ggttggcgat aatttcaacc tgatcccctg tctttaactt
     3181 ggttgtcagt ggaaccatgc ggccattgac cttggcacca gttgcttttt caccgacctt
     3241 ggtatggatt tcgtaggcaa aatcaatcgg tcctgaatct ttgggaaggg aacggacagc
     3301 tccatctggg gtaaaaacgt aaatctcctc agccaaatag ttttccttaa cagagtccac
     3361 aaattcctta gcatcatcag cctggtcttg gagctccatc atctccttga tccagttcat
     3421 tccaatagct gattccttgc tgttaacttg cccctttata cctttcttat aagcccagtg
     3481 agccgcaacc ccgtactcag ccacctcgtg catttccttg gttcgaatct ggaattcaat
     3541 cggccctttt ggtccataaa cagtcgtatg gatagactga taaccattgg ccttgcggtt
     3601 ggcgatatag tctttgaagc gacctggcat cggtttccaa aattcatgca cgtaaccaag
     3661 catggcataa acatcacttt gggtatctaa aatacaacga atagcaatca gatcatagat
     3721 ttcctcaaac cgttttctct tgtcctgcat tttgcggaaa attgagtaaa tatgcttggg
     3781 acgaccataa atcttccctt tcaagtgacg ttctgtcgta tactcctcta attttgtgac
     3841 tacctcatcc accaaggcct cacgctccct gcgcttttcc ttcatcatat gggtaatctt
     3901 gtaaaactcc gttggattga gataacggaa agacaagtct tctaattccc atttgacact
     3961 ggaaatcccc aaacgatggg caagcggggc atagatttcc atggtttctt tggaaatacg
     4021 ctcctgcttg tcttttcgaa gatgtttcag ggtccgcata ttgtgcaagc ggtcagacag
     4081 tttgaccaaa ataacgcgga tgtcctcaga catggccatg agcatcttgc gatgattttc
     4141 cgctaattgc tcctcgatcg atttgtactc gaccttgcca agcttggtaa ctccgtcaac
     4201 aatcacccgc acatcaggac caaactctct ttccaaatcg tccaaggtcg catctgtatc
     4261 ttccaccaca tcatgcaaga atccacaagc tactgttaca gcatccagct ttagcttagc
     4321 taaaatacct gccacttgga tagggtgaat gatataaggc tcgcctgatt tgcgatattg
     4381 accactgtgg cattcaacag catagaccaa ggccttatgg acaaaatgaa catcctcttc
     4441 cgttaaatat tctttggtta aagcgacaac ttcttcgcct gttaaattca cttctttcgg
     4501 catctctact ctccaattct tcctaccatt ttatcacttt tttaagaata tgaaaactag
     4561 attggaacag aataagaaaa aaataattca aaattgcttg ataattctga attattggcc
     4621 cataatatac tacgaagtta gattttaaac ttaggtgata gaaggagaga tagaagaacg
     4681 gaaaccatat tgtaacccaa agactttctg atttccccaa ttccattgaa gatacgaaag
     4741 ataaacggtg gaactcgtat cacatacact ggtaccttga ctggattttg gaattaatac
     4801 tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag gttgctcaaa gcactgcttt
     4861 gaggttgtag ataagactga caaagtcagt cacatatata cggcaaggcg acgttgacgc
     4921 ggtttgaaga gattgttgac gcggtttgaa gagatttttg aagagtataa aaatcctcaa
     4981 gatactttct tctatccttt agtttataag gagaatacct atgaaaaaaa ctgctatttc
     5041 tatctttgct ctcctaatgt taggagtttg ctgcctgttc ctattcagcc agcaaagcta
     5101 taaaaaactc gttcaatact atgctaacga ccagaacctg cccagtagga taacttatag
     5161 tgaatatagc gacaaatgat aagccaacta cggtagcact ctaaacatca cgtctatcaa
     5221 acaagctaat gacggagttt atgcaaccta tgaagggcaa ttgacacctt tccaatattg
     5281 ataaattgat aaccagcctg tcttcatcta gtcatgctgg tttttaagtt cattttaaat
     5341 ccttacctat tctccctaac tgtgctatac ttaatttata ctcaatgaaa atcaaagagc
     5401 aaactagaaa gctagccgca ggctgttcaa agcactgctt tgaggttgca gataaagttg
     5461 acgcggtttg aagagatttt cgaagagtat tagtacattc tttgagattg gagctagtat
     5521 gaaaatccat aaaaccgtga atcctgttgc ctatgaaaat acctattatc tagaaggcga
     5581 aaagcacctc atcgtcgtcg atcctggtag tcattgggaa gccattcgtc agacaatcga
     5641 gaagatcaac aaaccgatct gtgctattct cttgacccac gcccattatg accatatcat
     5701 gagtctggac ttggttcgcg agacgtttgg caatcctcct gtctatatcg cagagagcga
     5761 agccagctgg ctctacactc ctgtcgataa tctctccggt ctccctcgcc acgatgatat
     5821 ggcagatgtg gtcacaaaac ctgcagaaca cacctttgtc tttcacgaag aataccaact
     5881 agaggaattt cgttttaagg ttctaccgac cccagggcac tctatcggtg gtgtttccct
     5941 agtctttcct gatgctcacc tagtcttgac gggagatgct ctattccgcg aaactatcgg
     6001 acggaccgac cttccgactg gtagcatgga gcaactcctt catagtatcc agacccaact
     6061 cttcacccta ccaaactacg atgtctatcc aggacatggt ccagctacta ctatcgctca
     6121 cgaaaaggcc ttcaatccct ttttctagca agatgatgac aatcgaaatt taagtaaact
     6181 atccagcaaa tctttctatt acaaaaggca tcctatcaag gttttcacac gtgattggat
     6241 gccttttttc tgatgactag attttttgta ttaccaaata atcacgcgct cctctggtga
     6301 acgccacatt ccgtctcctt ctttgacatc ataggttgta aagaaatcgt cgaagtttgg
     6361 tacttgcaca ttgacacgga gtttggctgg tgcgtgcaca tcgacgctag ccaaaagttt
     6421 cataaattct ggtcgacctt tcatgcgcca gatgcgaccg aagttgtaga agaactcttc
     6481 tgctgagaag tctgcttctc tcttagctgc ttcaagcgct gctgcgattc ctcccaagtc
     6541 agccacgttt tctgatacag tcaatttacc gttaatggtt gctccataag aatcctgtcc
     6601 atcaaattgg tcaatgactt tttgtgtttt ctccttgaag gcagcatagt cgctctctgt
     6661 ccaccaatcc ttgaggctac cattttcgtc aaaggaagcc ccgttagtat caaaggcgtg
     6721 ggaaatttca tgggcaatca ctgccccaat accaccgtag ttagcagaag atgactgatg
     6781 caagtcatag aaaggcgcct gtaaaatggc cgctggaaag acaatcaggt tcttctgagg
     6841 attgtagtag gcattgacca tatgagcagg catgccccat tccttatagt ctacaggctg
     6901 gttccactta ctccaactgt gcttgatttc cacacgcgca aaggctagag cattctcaaa
     6961 aagactggca gtttcattca ctaccttatc cttgtaacgt gcaggcaatt cttctggata
     7021 gccaatataa ggtttgatca cattgagctt cacgatagcc tgtttacagg tttctggagt
     7081 gagccagtca ttcttaagca gacgctcctt atagacatca atcatggttg ccactttttt
     7141 ctccacatcc gccttggctt ctggagagaa cttctcacgg gcgtaccaaa gacccagggc
     7201 ttgcttgaaa ggttcttgtg ctagatgata agctgctttg accttatctt ttgcctctgg
     7261 aactccagaa agggcacggc tgtaggcacc agacaaaaca cggatatcct ctgttaaata
     7321 gctggttgaa agattgacaa cactcaaaat caaggttgct ttaaggagag accaggcttc
     7381 ctcactgtag aattgctctg ctgcttgcca gaaacgttcc tcgtctacaa taaccttgtc
     7441 tggtaattgc ccaataactg ctttgaagaa gtcatccaaa ggtagggcag gcgcgaattt
     7501 cttaaaatct tcgtaagaat atggatgata gagtttagca tattctgaac tttcttcatt
     7561 agagagcacc actgccgcaa ctcggcggtc caattcaagt cttttttcta gcaagtcttc
     7621 aatttcttca tcagagaaat cataagcctt gaggagattt gcgctgcttt ctttccaaag
     7681 agtcaagagc tcttcgcgct gaggatgttc ttctgcatag taggtcgtat ctggcaagat
     7741 tgtgcttgga gcgctagccc atagaacatt gattctagca tccataaagt ctggcgatac
     7801 accaaaagga aggaagtttg gttttcctgc aagctcaaac tctgctagtt tagctgtaaa
     7861 atccgcaaaa gtttccaatt cttggaattc tttaaggagt ggtaagacag gtgtgatacc
     7921 gtcagcttct ctcttgtcaa aatcacgaac taggcggtgg tatttgacaa agttttccaa
     7981 gatagcatcc tcaggcactt cttcacctgc taaccacttg tctgttgtcg ccagcatcag
     8041 gtcttcaatt tcctggtcta aatcaacaaa acctccagtt tgagacttat ctgctgggat
     8101 ttcagctgtc tgttgccatt ctccattgat agcatcataa aaatcatctt gataacgtgt
     8161 catcttgttc tcgctttcat ttgtatttgc atttatctta acaaaaatca ctagggaatg
     8221 caattaataa ccatttaact attatttttt agctattata aaaaatcttt ccatgataaa
     8281 acg
//
LOCUS       DAVKQB010000072         8211 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKQB010000072 DAVKQB010000000
VERSION     DAVKQB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8211
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.072"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..119
                     /locus_tag="VW528_001934"
     CDS             <1..119
                     /locus_tag="VW528_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEU9912704.1"
                     /translation="NVSEVISALDIIHHLDKIAHTTQAIYKWIMYRKYGNIN"
     gene            132..1607
                     /gene="galT"
                     /locus_tag="VW528_001935"
     CDS             132..1607
                     /gene="galT"
                     /locus_tag="VW528_001935"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9912705.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1620..2630
                     /gene="galE"
                     /locus_tag="VW528_001936"
     CDS             1620..2630
                     /gene="galE"
                     /locus_tag="VW528_001936"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9912706.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSKLVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNIFE
                     SVNKIPIPYKIVPRRSGDVATYYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            2794..3453
                     /locus_tag="VW528_001937"
     CDS             2794..3453
                     /locus_tag="VW528_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9912707.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3478..4545
                     /locus_tag="VW528_001938"
     CDS             3478..4545
                     /locus_tag="VW528_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9912708.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4649..5659
                     /locus_tag="VW528_001939"
     CDS             4649..5659
                     /locus_tag="VW528_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912709.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5671..7362
                     /locus_tag="VW528_001940"
     CDS             5671..7362
                     /locus_tag="VW528_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9912710.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7365..8075
                     /locus_tag="VW528_001941"
     CDS             7365..8075
                     /locus_tag="VW528_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9912711.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2776 a         1139 c         1618 g         2678 t
ORIGIN      
        1 ggaacgtttc tgaagtaatt agtgctttag atattattca tcaccttgat aaaattgctc
       61 atactacgca agcaatttat aaatggataa tgtatagaaa atatgggaac ataaattaga
      121 aagaaaaata tatgaaaaca attattgata attttgttga tagagtaatt gaatacggaa
      181 tgtataatga aattgacaaa atatatgtga aaaatagagt actagcgtta ataggtgagg
      241 agggaattga tcgcatttcc gatgaaaatg acctgaaaca aataaaagat tacttagtgg
      301 aaatcgcctt aaaaaacggt aaaattaaag atttaatcga ggaaaaggaa tgtttaggag
      361 cagagttaat gaatttcata gttcctttac ctagtcgatt aaatgatatt ttttggagtt
      421 catatgatat atcacctcaa gaagcagttg aagagtttta taagttaagt aaagatagtg
      481 attatattaa aacttctgct attgctaaga atattgagtt tcgagcatca actaaatacg
      541 gagagctaga gattacgata aatttgtcaa aacccgaaaa agatccaaaa acaattgctg
      601 ctgaaaaatt agtaaaagca actaattatc ccaaatgttt actatgtatg gaaaatgaag
      661 gatatcaggg gcgaataaat tatccagcac gttcgaatca tagaattatt cggttgaaac
      721 ttggtgacga agtatgggga ttccagtatt ctccctactc atactttaat gagcatgcga
      781 tatttttgaa tagtcaacat gtaccaatgg ctattacctc taaaacattt gaacaattat
      841 tggagattgt tgatattttg ccaggatatt ttgctggttc aaattctgat cttcctatct
      901 caggaggctc cattcttagt cataaccact accagggagg gaaacatatt ttcccaatgg
      961 aaaaagcaaa atttgagagt gaattttgtt ttaaagactt tgaggatgtt aatgctggta
     1021 ttgtaaaatg gccaatgtca gtaattaggt tacaaagtga aaataaaaat cgcttgttag
     1081 atttagctac taaaattcta aataagtgga gagaatattc agatttagaa gtagacatca
     1141 ttgctatgac tgaagatgtc ccacaccata cggtgactcc tattgctcgt aaagtggacg
     1201 ggagatacga attagatatt gttctaagag ataatcatac tactgaacaa tacccagatg
     1261 gtgttttcca tcctcatcaa gatgtacaac atattaaaaa ggaaaatatc gggttaatcg
     1321 aagtgatggg acttgcgata ttaccacctc gtttgaaacc agagttagaa gaagttggaa
     1381 aatatctttt aggagaggat aatgcaattg cggattatca tttagaatgg gccgaccaat
     1441 tgaaagaaaa atatcctcgc attaataaag aagaggttaa tagcgtagtt caacacgaag
     1501 caggacaagt gtttgcgaga gttctggaag atgcaggagt ttataagaat acaccgtctg
     1561 ggcatgaagc gtttatgaga tttgttaagt cggttggaat taattaggag gaaacccaga
     1621 tggcaatatt ggtaacaggc ggagctggtt atattggtag ccataccgta gtagaattac
     1681 taaatttagg aaaggaagtt atcattgtcg acaacctttc gaactctagc aaattggtgc
     1741 tagaccgtat tgaagcaatt acaggaatac gtcccgtgtt ttacgaatta gatgtttgtg
     1801 ataaacaagc attgagaaag gtatttgaac aagaatcgat tgatgctgca attcattttg
     1861 caggttataa agctgtcgga gaatccgtgc aaaagcctgt gatgtactac aaaaataata
     1921 ttatgagtac attggcactt gttgaagtga tgtcagaatt taatgttaaa aagattgtct
     1981 tttcttcaag tgcgactgta tatggaatta acaatcagtc acctctaatt gagacgatgc
     2041 aaacaagtgc gacaaatcct tatgggtata cgaaagtgat gcttgagcaa attttaaaag
     2101 atgttcatgt ggcagattca gaatggagta ttgcgttgct tcgttatttc aatccaattg
     2161 gtgctcatga gtctggtttg attggggaag atccctcagg aattcctaac aacttgatgc
     2221 cttatattgc acaagtagcg gtaggtaagc tatcagagct aagtgtgttc ggaaatgatt
     2281 atgatacgct tgatggtact ggtgtgcgtg attatatcca tgtagtagat ttagcaatag
     2341 gacatataaa agctttagaa aaagtatctg aaaaaacaga tgtttatatc tataacctag
     2401 gctcgggaga aggcactagt gtattacaac ttgtaaatat atttgaaagt gttaataaga
     2461 tccctattcc ttataaaata gttccaagac gttcaggaga cgttgcaact tattatgcaa
     2521 atgcagacaa agcatataag gaattaaatt ggaggacaac aaaatcgatt gaagacatgt
     2581 gtagagatac atggaattgg caatcaaaaa atcccaatgg ctataattga tgatactgtc
     2641 tatatgagtg ctgtattttg gaaaaattac ttaacctttt aatgaaagat aaattaaaaa
     2701 aaatgtttga cagcgcttga caaaggaaac ggtttcattg tataatgtaa acacaatgac
     2761 accgatgtca aatataaaaa agaaggtctt tatatgaaaa acttaacaaa aattaagttc
     2821 aaagaaaatg gggaatttaa tcatttccct gggaatacag ttgtagcaaa tctttatact
     2881 aaacaagatt tgatggaagt tgttgatatt attcaatcac gttatagaga attaccattt
     2941 atcgataagt ttactttaac tccaaggaat tctattcaca tgactgtaat tgaattgttg
     3001 tgccatgaaa atcgtgaaac ggagttttgg agcagtaatc ttcctctaga tacaccttta
     3061 caggaaatac atgattactt tgctaaacaa cttgaaattt ttccattgtt ggatgaagaa
     3121 attcatatgc gtataactga aatgggaaaa caaaacatac tagttgaacc tgcagatgaa
     3181 gcttctgcaa agagattaga agaaattcgt acttatgtct cagaaaaagc aggtgtttgt
     3241 ttccctaatc atgatagata tcaattccat atttcaattg ggtatcttcg gattcctcta
     3301 accgaagagg aggaagaaga gtttactaaa gtcagagcag aattaactga aattttatta
     3361 gagaagattc caactattac tgtaaaccgt attgactata ctgtatttga agatatgaga
     3421 caatttgttc catatcacga aaaatttaaa taaaataata aaaaaggaga acttatcatg
     3481 aaaaaaatga aagtttggtc tactgtactt gcaacgggag ttgctcttac tacacttgct
     3541 gcttgctctg gaggttcaaa ttctacgact gcttcttcat ctgaagaaaa agctgataaa
     3601 agtcaagaat tagttatcta ttcgaactca gtctcaaatg gtcgtggtga ttggttaact
     3661 gctaaagcaa aagaagctgg ttttaatata aaaatggttg atatcgctgg cgctcaatta
     3721 gcagaccgtg ttattgctga gaagaataat gcagttgcag atatggtatt tggaattggt
     3781 gctgttgact caaataaaat tagagatcaa aaattactag tacagtacaa gcctaaatgg
     3841 ttagataaaa ttgatcaatc tttatcagat aaagataatt attataatcc tgtgattgtt
     3901 caaccattag ttttaattgg ggcgcctgat gtaaaagaaa tgcctaaaga ttggactgaa
     3961 ttaggtagta agtataaagg taaatattca atttatggtc ttcaaggtgg tacaggacgg
     4021 gcaattctag caagtatctt agttcgttac ctggatgaaa aaggagattt aggcgtatca
     4081 gaaaaaggat gggaagttgc aaaagaatac tttgcaaatg cctatactct tcaaaaaggt
     4141 gaaagctcag ttgttaaagc gttagataaa gaaagcccaa ttcaatacgg tatgatctgg
     4201 ggttctggag cgttaatcgg acaaaaagaa caaaatgttg aattcaaagt tatgactcct
     4261 gagattggtg taccatttgt aactgaacaa actatggttt taagcactag taaaaaacaa
     4321 gcgttagcta aagaatttat tgattggttt ggtcaatcag aaattcaagt agaatatagt
     4381 aaaaaatttg gatctattcc tgcaaataaa gatgccctaa aagagttacc tgaagatacg
     4441 aaaaaatttg ttgatcaagt gaaaccacaa aatattgact gggaagctgt tggaaagcat
     4501 ttggatgaat gggtagaaaa agctgaatta gaatacgtaa aataagaaat aagagttatt
     4561 ttttatgtcc aagtctctct attatttcta aatttaagga agaaataaag agaggctttt
     4621 ggttttagta atagagggag ttagtgagat gattaaattt gataatattc aaattaaata
     4681 tggtgatttt gttgcaattg ataatctgaa tttagatata catgaagggg aattctttac
     4741 atttcttggg ccttcaggat gtggtaaatc aactactttg agagcattgg taggtttttt
     4801 agatccatca tcaggaagta ttgaagttaa tggaacagat gtcactcatt tggaacctga
     4861 aaagcgtggg attggtattg tatttcaatc ttatgcgcta tttccaacta tgactgtttt
     4921 tgataatatt gcatttggtt taaaagttaa gaaggtagct ccagatgtta ttaaagctaa
     4981 agtatcagca gtggcagcaa aaattaagat ctctgatcaa cagttacagc gtaatgtatc
     5041 agaattatct gggggtcaac aacaaagggt agcattggct cgtgctctgg ttcttgaacc
     5101 taaaattctt tgtctagatg aaccattgtc aaaccttgac gcaaaattac gtgtagattt
     5161 gagaaaagag ttgaaaagac ttcaaaaaga gttaggtatt actactttat atgttactca
     5221 tgatcaagag gaagccttga ctttatctga tagaattgca gtctttaaca atggatacat
     5281 cgaacaggtc ggtacaccag tagagattta tcataattct caaactgaat ttgtatgtga
     5341 ttttattgga gatattaatg ttttgaccga tgaaacagtc cacgaagtat tattgaaaaa
     5401 tacaagcgtt ttcttagagg ataaaaaagg atacattcga ttagagaaag ttcgattcaa
     5461 tcgtgaaact gaacaagatt ttattctaaa agggacaatt attgatgttg agttttctgg
     5521 agttacaatt cactatacaa taaaagtttc tgaaagtcag attcttaatg taacaagtat
     5581 tgatagtcag gctgctatta gatctgtcgg agaaagtgtg gaattattta tcacaccatc
     5641 agacgttctg caattttaag gaggtgcagt atgcgtcata aattaaattt aaaagattgg
     5701 cttattcgtt tagggttaat ctggttctta gtaacattta ttatttatcc aaactttgat
     5761 ctagtagtga atgtatttgt aaaaggagga gaattttccc ttgatgctgt acatcgtgtt
     5821 ctaaaatctc agagggcact tcagagtatt atgaacagtt ttaagttagc attttcactc
     5881 attattacag ttaatgtcgt aggtattctt tgtgttctat ttacagagta ctttgatatt
     5941 aaaggtgcta aaattttaaa attaggttat atgacctctt taatttatgg aggagtggtt
     6001 ttagcgactg gatataaatt tgtctatggt ccttatggat tgattacaaa atttttacaa
     6061 aatgttatcc cttctttaga ctctaactgg tttattgggt atggtgcagt cttattcatt
     6121 atgacatttt caggaactgc taatcataca ttgtttttaa caaatacaat tcgaagcgtt
     6181 gactatcaca ctattgaggc tgctcgaaat atgggagcaa aaccatttac tgttttccga
     6241 aaagtagtgt taccaacctt aattccaact ctatttgcac ttactattat ggtttttctt
     6301 agtggtttat ctgcagtagc agcacccatg attgttggtg gtaaagaatt tcaaactata
     6361 aatccaatga ttattacatt tgcagggatg gggaattctc gtgatttagc tgccttactt
     6421 gcaattattt taggtattgc aactacaatt ttgcttacta tcatgaataa gatagaaaaa
     6481 ggtggaaatt atatttctat ctctaagact aaagcgcctc ttaaaaaaca aaaaattgcg
     6541 tctaagcctt ggaatatcat tgctcacatt gtagcatatg gattgttcac agttttcatg
     6601 cttccactaa tttttatagt attatactca tttacagatc cagttgcaat tcaaacaggt
     6661 aacttaacat tatcaaactt tactttagaa aattatcgct tattctttag taatagtgcg
     6721 gcattctctc cattcttggt cagctttatt tattctatta ttgctgcgac aacagcaaca
     6781 attctcgcag ttgtatttgc tcgtgttgtc agaaaacata aatctcgttt tgatttctta
     6841 tttgaatatg gtgctctact tccttggtta ctaccaagta cacttttagc agtaagttta
     6901 ttatttactt ttaatcagcc acaatttctt gttttgaatc agattttggt aggtagtttg
     6961 gtaattctac ttattgcata tatagttgta aaaatcccat tttcttatag aatggtacgt
     7021 gctattttat ttagtgttga tgatgagatg gaagatgcag caagaagtat gggtgcttca
     7081 cctttttata ctatgatgaa ggttatcatt ccatttattt taccggttgt tctctctgtt
     7141 attgctttaa actttaactc tttattaact gacttcgact tatctgtatt cctttaccat
     7201 cccctagctc aaccattagg tattacgatt cgatctgcag gtgatgaaac agcaacatct
     7261 aatgcacaag ctctggtatt tgtttataca attgttctga tgattatttc tggaacggta
     7321 ttatacttca cacaaagacc ggggcgtaaa gtaaggaaat aatcatgaca gccactagtc
     7381 ttgggttatc aaatattgaa atagttgtca ggattgtttt atcagtagtc attggtagta
     7441 taattggttt agagagaggg agcaaatccc agcctgcagg catccgaact tatagtattg
     7501 tttgtctagc tgcatgtttg attatgatga cgaatgaata cgtatcttat aaatttggga
     7561 caggagatcc tacacgatta ggagctcaag ttatatcagg tgtgggtttt ctaggcgctg
     7621 gaacgattct tattacagat aagaagaaaa ttacaggtct gacaactgca gcaggcattt
     7681 gggcttcggc aggaattgga ttagctattg gagtaggttt ttatgaggga gctcttttag
     7741 tagccatttc tgtttggggt gtgatatcca tgttccaacc actaaaaaaa tatctgcaaa
     7801 atcgttctaa aatgattgaa ttgtatatag tagttaaatc tacagaagcc tataatcgtg
     7861 tattggttta ttgtgcagaa aatggtatca gaatgaccga ttcaagaaca gcgtttggag
     7921 atgttaattc tgacagaatt gaatactttg atgttccgga caaaaaaata gcgtcattta
     7981 ttactctgga attatcaggt agatttgaac atcttcgact gatggaagaa attgctaata
     8041 ttgtaggtgt tatttatgtc gaagaaatta gctgaaattt agtaaacaaa gggacagatg
     8101 tttttgtcct tttgtttttt agatgtttta atcatctaat ttttgagtag atttttgctc
     8161 agaggttttt atgaaagatt ataaaatcaa ttttgattta ggtaaaatag a
//
LOCUS       DAVKQB010000073         8154 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKQB010000073 DAVKQB010000000
VERSION     DAVKQB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.073"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(46..1290)
                     /locus_tag="VW528_001942"
     CDS             complement(46..1290)
                     /locus_tag="VW528_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9912712.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(1309..1914)
                     /locus_tag="VW528_001943"
     CDS             complement(1309..1914)
                     /locus_tag="VW528_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9912713.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            complement(1974..2897)
                     /locus_tag="VW528_001944"
     CDS             complement(1974..2897)
                     /locus_tag="VW528_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9912714.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            3191..4570
                     /locus_tag="VW528_001945"
     CDS             3191..4570
                     /locus_tag="VW528_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9912715.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            4722..5597
                     /gene="pdxS"
                     /locus_tag="VW528_001946"
     CDS             4722..5597
                     /gene="pdxS"
                     /locus_tag="VW528_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9912716.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            5598..6182
                     /gene="pdxT"
                     /locus_tag="VW528_001947"
     CDS             5598..6182
                     /gene="pdxT"
                     /locus_tag="VW528_001947"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9912717.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEDVEILATVDNQIVA
                     AQEKNMLVTSFHPELTDDAHLHQYFIEMCKEKRQ"
     gene            6739..7470
                     /locus_tag="VW528_001948"
     CDS             6739..7470
                     /locus_tag="VW528_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912718.1"
                     /translation="MVNNVAVKVSNLSKEFLLGQDKTVSILKDISLSVNYGEFVSILG
                     VSGSGKSTLLSCLSSLSEPTSGEVVINGVNPYTLKEGKLAKFRRQDIAIIFQNYNLVP
                     ALPVLENVTLPLRLSGKSVDSNKVKKMLDSLNFKAELSSLVATLSGGEQQKVAITRAI
                     IADSKIIFADEPTGALDSVSRKLIFETLRNLASQGKCVLMVTHDIELASKTDRALILK
                     DGKISRQIIKPSADELYQALESSKD"
     gene            7488..>8154
                     /locus_tag="VW528_001949"
     CDS             7488..>8154
                     /locus_tag="VW528_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-carotene 15,15'-monooxygenase"
                     /protein_id="HEU9912719.1"
                     /translation="MLKLIIYQFQYSKRQWLGTIPLLFVSSLIVGTSLFGIASSIKTA
                     NINASQLFQMLIIFGGTTLFFLISNNIRLLIDIFKKDYQLWTILGASRTQLSLLVSGQ
                     FYLMAVIVSSIGTILSFIMADSYYKFLQNLLGRDELPDLVITANIQSILLSIFIVPTI
                     VGIGAYFYSSRILKISSILKPKKKKRKVTVTGFVNISVRLFLWLLCIGSIVSAGFIRN
                     KEII"
BASE COUNT         2506 a         1470 c         1685 g         2493 t
ORIGIN      
        1 ttttttcgct aaaaaaggaa ccataatggt tcctaaaaac tatcattagt aacttgcacc
       61 ggctgtagcg tctgcgtcac caccgtggcc tccagcatcc cctgaatcag aagcgccaga
      121 agtagcatcg gcgtctccat gacctccggc agcaggagca aatggtccgc taccacccac
      181 caaacgttga ccagtctctt ttaggtacca gtcaagccat ggttggaagt taaagacgat
      241 ttcattgata ccagcgtatg atccatcagg atagtacatt gcttggtagt tgtgagtgtt
      301 gataacacct gcaggagaac ctggaacgat cgtacggacg tattcttggt ttccgttgcg
      361 aagtgttccg ataacccact ctacgttctt catacgtgct ggtggaagag aaccatgaac
      421 agtcgacata cggctacctg attgaggtgg tacacgttta gcgaacatag tgtctggatc
      481 ttggtgagcg ttgttgtagt agaggaattg gttgttgtcg tcagcgtatg tcaattcaaa
      541 tggcatagct ttcaagaaca tatcaatttg gttaactgtt aggataccgt ggtccaattt
      601 gacataggta tcaccagaaa cagcaccagt gaatgctgca actttttcta cccattctgg
      661 atcgtcaggg tcaacttctg tgatggttgt agcgattggt tttccacaat ccaagtcttc
      721 tgattcgatt ggttttggtt ttttcaattt cgaaacgact cctacgtatt taacaaagtt
      781 atctaagcaa gtttcaagga atttaacagt gccttcgttg gtgatatttc cgttgttatc
      841 aaaagcttcc ttagctttac caagaaggaa ttcgttacct ggaagcgtgt aggcattaac
      901 acctggagca tcaaggattt tacgaaggtg aacttgagca cgtgatgttc cttggtcata
      961 gtatgatgca cccacaatca taacaggctt gttttcaaat ggatgaactt cgtatgaaag
     1021 ccattcaagt acagatttga gtgaagctga gatagtgtgg ttatgctcag gagtagcaat
     1081 gataacacca tctgcacgag taattttgtt atataaataa cgtaattgga aactttcatc
     1141 ccatttttca tcttggttaa acattggaac ttcgtcaatt tcaagaactt ctaattcaaa
     1201 tttgagtttg aagtagcgac ggataaattc caagagctta cggttatatg attgatcgta
     1261 gtttgatcca acaagtccaa caaatttcat tctttttggt ctcctatctt acaaattttc
     1321 ccagtcaaag tcttcagcat ctttgcgaag taattcttgt gcattacgta atttttctgt
     1381 gatttttaca aagatacgga agtcatcaaa gatggcatcc aatttcttga taacatcaag
     1441 gtcaaccaag tcgccacttg ggttaaatgc ttgaagagag tgtgagagca agaattcatc
     1501 tggaagaaca tttgccttga tttcaggagc attcaagatt tgacgaagtt gcaattgggc
     1561 acgagatgaa ccaagcgtac cgtaagaagc acctgtaatc atgattggtt tgttcaaaag
     1621 tgggtaaata ccataagata accaagcaag agcgctcatc aaaacagctg gaatagagtg
     1681 atcatactca ggagtaccga taataacgcc atctgcctct tcgattttag cagcaatttc
     1741 caatatttca gcaggtactt gcttgtcagc tggtttgttg aagacaggaa tggccttgat
     1801 ttcaacaagt tcaatttcag ctttgtcagt aaagtgtttt tgcatgtatt gaagcaattg
     1861 acggtttgta gaacgttttg aatttgttcc aacaatagca ataagtttta acatgagatt
     1921 tcctttctct ttttacataa tacaatttta aaattccatt gaaacagttg tctctataga
     1981 gtaggaattc ctgaagaaca gcttaggtgg ccttctttat cgatgaggat gacttcgatg
     2041 ccctccaaac tttcgacttg ccagaggata gaagcaggtc tttctccaaa gagtcgagtc
     2101 gtccagattt cgccatcgac tgatttatca gagatgattg ttagactcgc tagttccgtt
     2161 tcaacaggat atcctgtttg actgtcaaaa atgtgatggt aatcttgtcc atcgacggtc
     2221 aggtgacgtt cataaatgcc tgaagtcacg acagatttat tgacaacagg gatggtcatt
     2281 aaatgatttc ccctaggatt ggctgggtct tgaatcccga tttgccatgg gttatcccct
     2341 cttgcctgat tttttccaat ggtcaggata ttccctccca gattgatcaa ggcagaagtc
     2401 accccctctt tcctaagaaa ttgggcaacc ttatccgcac tgtatccttt ggctaaacaa
     2461 cctagatcga tcttcattcc tttctgtttt aaaaacacag tagaagtaga agaatctaac
     2521 tcgataccat gaggattgat tagaggcagc accgattcaa tttcttgagg ctgggcgacc
     2581 ttggcatctg aaaaaccgat acgccaggtt tgaattaagg gaccaatgct gatattgagg
     2641 tggctagaga gcgctaggct atgctctaac ccaagtgaaa tcagctcaaa caggtctgga
     2701 tgaaccgtga cgggggctat tcctgcttga taattgattt ccatcaactc agattcttga
     2761 ctattggcgt tgaagcggta ttcaagttct ttgagcaagt caaaggattt ttggagaaag
     2821 atatcggctt gctcatccac taatgaaata gtgatagtag tccccattag ccgttcagaa
     2881 tgtgaacgaa gagtcaagct accaactcct ttctcttata gaaaataagt tgtaatatca
     2941 aataatcatc taaattgaag cccttacatt tcattttcat gttattataa taccataaag
     3001 ttagaatttt cacaaacaaa atttggaaaa agtcaagaaa tatgctcata aaattcatca
     3061 ggcttgaaaa caggataaat ggggaattat ttttgataaa aaatgctgaa ataatagtac
     3121 cccccttgta aacgctaacg gtaaatggta tactagtaag gtaaatttag aatgaaggca
     3181 ggaaattttt atgagtaaaa tcgttgtagt cggtgctaac cacgctggta cagcatgtat
     3241 caataccatg ttggataatt ttggaaatga gaacgaaatt gttgtatttg accaaaactc
     3301 taacatctct ttcctaggat gtggaatggc tctttggatt ggtgaacaaa ttgacggtgc
     3361 tgaaggcttg ttctattctg ataaagaaaa attggaagct aaaggtgcta aagtttacat
     3421 gaactcacct gttctttcaa tcgactatga taacaaagta gttacagcgg aagttgaagg
     3481 aaaagagcac aaagaatcat acgaaaaatt gattttcgct acaggttcta caccaatctt
     3541 gccaccaatc gaaggtgttg aaattgttaa aggaaaccgc gaatttaaag caactcttga
     3601 aaacgtacaa ttcgtgaaat tgtaccaaaa tgctgaagaa gttatcaata aactttctga
     3661 caagagccaa cacctcgacc gtatcgccgt tgttggtggt ggttacatcg gtgttgaact
     3721 tgctgaagcc tttgaacgtc ttggaaaaga agttgtcctt gttgatatcg ttgatactgt
     3781 cttgaacggt tactatgaca aagacttcac acaaatgatg gcgaagaact tggaagatca
     3841 caacatccgc ttggctctag gtcaaactgt taaagcaatc gaaggtgacg gtaaagttga
     3901 acgcttgatt actgacaaag aaagctttga cgtggatatg gttatccttg cagttggttt
     3961 ccgtccaaac acagcccttg caggtggtaa gatcgaactc ttccgcaacg gtgccttcct
     4021 tgtagacaag aaacaagaaa catctatccc agacgtttac gctgttggtg actgtgcgac
     4081 tgtttatgac aatgctcgta aagatacaag ctatatcgct cttgcttcaa atgctgttcg
     4141 cactggtatc gttggtgcct acaatgcttg tggacatgaa ttggaaggaa tcggtgttca
     4201 aggttcaaat ggtatctcaa tctacggtct tcacatggtt tcaactggtt tgactcttga
     4261 aaaagcgaaa gctgctggtt acaacgcaac tgaaacaggc tttaacgatc ttcaaaaacc
     4321 agaattcatg aaacatgaca accatgaagt agcaattaag attgtctttg acaaagatag
     4381 ccgtgaaatt cttggtgccc aaatggtttc ccatgatatt gcaattagca tgggaatcca
     4441 catgttctca cttgctatcc aagagcatgt gacaattgat aaattggcat tgacagacct
     4501 cttcttcttg ccacacttca acaaaccata caactacatc acaatggctg cccttacggc
     4561 tgaaaaataa aaatgaatga gctatctggc cttaagttaa ggtcagatag tttttagcta
     4621 atctgtcccc atacaattat agttttttta tcttgtgctt cattctgttc tgacttaaaa
     4681 tgaaaaggta gctaccaata caaatgatga ggataaaaca aatgactgaa aatcgttatg
     4741 aactaaataa aaacttggca cagatgctca agggtggtgt tattatggat gtgcagaatc
     4801 ctgaacaggc tcgtatcgca gaagctgctg gtgcggcagc tgtgatggcc ttggaacgaa
     4861 ttccggctga tattcgtgca gctggaggag tttcccgcat gagcgaccca aagatgatta
     4921 aggaaatcca agaagcggtt agtattccag taatggctaa ggtcagaatc gggcattttg
     4981 ttgaagctca gattttagag gctattgaaa ttgattatat cgacgagagt gaagttctat
     5041 ctccagctga tgaccgtttc catgtggaca agaaagaatt ccaagttcct tttgtctgtg
     5101 gtgctaagga tttgggtgaa gccttgcgtc gtatcgctga aggtgcttcc atgattcgta
     5161 ccaaaggaga accagggaca ggggatatcg tccaagctgt tcgtcatatg cgtatgatga
     5221 atcaggaaat tcgccgcatt caaaacttac gtgaggacga gctttatgtt gctgccaagg
     5281 atttgcaagt ccctgtagaa ttggtccaat atgttcatga acatggaaaa ttgccagttg
     5341 taaatttcgc tgctggaggt gttgcaacgc cagcagatgc tgcgttaatg atgcaactag
     5401 gggcagaggg ggtctttgtc ggttcaggta ttttcaagtc aggagatcct gttaaacgag
     5461 cgagtgccat tgttaaggct gtgactaact tccgtaatcc tcaaatccta gctcaaatct
     5521 ctgaagattt aggagaagcc atggttggta ttaatgaaaa tgaaatccaa attctcatgg
     5581 ctgaacgagg aaaatagatg aaaatcggaa tattggcctt gcaaggggcc tttgcagaac
     5641 atgcaaaagt gctagatcaa ttaggtgtcg agagtgtaga actcagaaat ctagatgatt
     5701 ttcagcaaga tcagagtgac ttgtcgggtt tgattttgcc tggtggtgag tctacaacca
     5761 tgggcaagct cttacgtgac cagaacatgc tacttcccat ccgagaagcc attctatctg
     5821 gcttaccagt gtttgggacc tgtgcgggct taattttgct ggctaaggaa atcacttctc
     5881 agaaagagag tcatctagga actatggata tggtggtcga gcgtaatgct tatgggcgcc
     5941 aattaggaag tttctacacg gaagcagaat gtaagggagt tggcaagatt ccaatgacct
     6001 ttatccgtgg tccaattatc agcagtgttg gagaagatgt agaaattcta gcaacagttg
     6061 ataatcaaat cgttgcagcc caagaaaaaa atatgttggt aacttctttt catccagaat
     6121 tgacagatga tgctcactta caccagtact ttatcgaaat gtgtaaagaa aaaagacagt
     6181 aaaaaagctc aaaaaatcac ttcctgtgct atactgtaat tgaaattttt ttagtttttg
     6241 cgttagaact gtttcctcag tcctagcgcc ttttcttttt gataagtctg cttggtggag
     6301 agtagtttgt aaacgattct gagaatctta tgagcacaag caatgacggc tttcatcttg
     6361 cttcctcttt gggaaattcg attgtaaaac tctaagcagg cagagcctgc ttagagtttt
     6421 acatatgcta ctatctttgt tatagctaac ttaaattctc atttattaag tatacctaag
     6481 ttttatgttt aaattttgta tagaatagtt gtatcactta acaaagctat gtttacgctt
     6541 tcaatcaagt ctcatttatg atactattat ggtaatagaa aaaattctta gttcgctaaa
     6601 gacctttatt cgagatccag aggaaataga aattttatcc attaaaaata cgtttcataa
     6661 gttacttact tgatattaat agaattccaa tattgtataa ttaaatcaaa gatgacactg
     6721 gaatcaggag gatttattat ggttaataat gtagctgtta aagtctcaaa cctttcaaaa
     6781 gaatttttgt tagggcaaga caaaactgtc tctattttga aagatatttc cttatctgtc
     6841 aattatgggg aatttgtatc aatacttggt gtcagtggtt ctggaaagtc tactttgcta
     6901 agttgcttat caagtttatc tgaaccaaca agtggcgaag ttgttatcaa tggtgtaaat
     6961 ccatatacct taaaagaagg gaagcttgct aaatttagaa gacaagatat tgcaataatt
     7021 tttcaaaact ataatctggt acctgctcta cctgtactag aaaatgttac acttcctctg
     7081 agactatcag gaaagagtgt tgatagcaat aaagtaaaaa aaatgttgga tagtttgaat
     7141 tttaaagcag aactatcatc attagttgct accttatcag gtggagaaca gcaaaaagtg
     7201 gctattactc gtgcaataat agctgatagt aaaattattt ttgctgatga gccaacagga
     7261 gctctggaca gcgtctcaag aaaacttata tttgaaacac ttcgtaattt agcgagtcaa
     7321 ggaaaatgtg ttcttatggt cactcatgat attgagttgg cttcaaaaac tgacagagca
     7381 ctcattttaa aagacggaaa aatatctcga caaattatta aaccttcagc tgatgagctc
     7441 taccaagcac ttgaaagtag taaagattaa tttgaggagg atttattatg ctaaaattaa
     7501 tcatttacca atttcagtac tcaaaaagac aatggttagg aacaatacca ttactatttg
     7561 tatctagcct gattgttgga acatctttat ttggtattgc tagttcaatc aagactgcta
     7621 atattaacgc ttcacaactt tttcaaatgc taataatttt tggcgggact actctatttt
     7681 tccttatttc aaataatata agactgctaa tagatatctt taaaaaggat taccaattat
     7741 ggactatctt aggcgcaagc agaactcagc tatctttatt agtatctgga cagttttatt
     7801 taatggcagt gattgttagt agtattggta caatactttc atttatcatg gcagatagtt
     7861 attacaaatt tttacaaaat ttattaggaa gagatgagtt acctgattta gttattacag
     7921 ccaacattca atcaatttta ttgagtattt ttatagtccc aacaattgtc gggatagggg
     7981 cttactttta ttcaagtcga atacttaaaa tatcatcaat attaaaacca aaaaagaaaa
     8041 aaagaaaagt tacagtaact ggatttgtta acatctctgt tcgtttattc ctatggctac
     8101 tatgtatagg ttctattgtt tcagcaggtt ttatcagaaa caaagaaata attg
//
LOCUS       DAVKQB010000074         7741 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKQB010000074 DAVKQB010000000
VERSION     DAVKQB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.074"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..459
                     /locus_tag="VW528_001950"
     CDS             <1..459
                     /locus_tag="VW528_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9912720.1"
                     /translation="RYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLK
                     HHYQIYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHY
                     SNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(494..916)
                     /gene="nusB"
                     /locus_tag="VW528_001951"
     CDS             complement(494..916)
                     /gene="nusB"
                     /locus_tag="VW528_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9912721.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREYT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(909..1298)
                     /locus_tag="VW528_001952"
     CDS             complement(909..1298)
                     /locus_tag="VW528_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9912722.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(1320..1880)
                     /gene="efp"
                     /locus_tag="VW528_001953"
     CDS             complement(1320..1880)
                     /gene="efp"
                     /locus_tag="VW528_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9912723.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(2009..3451)
                     /gene="gatB"
                     /locus_tag="VW528_001954"
     CDS             complement(2009..3451)
                     /gene="gatB"
                     /locus_tag="VW528_001954"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9912724.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(3451..4917)
                     /gene="gatA"
                     /locus_tag="VW528_001955"
     CDS             complement(3451..4917)
                     /gene="gatA"
                     /locus_tag="VW528_001955"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9912725.1"
                     /translation="MTFNDKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDTDNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSASVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILSAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(4917..5219)
                     /gene="gatC"
                     /locus_tag="VW528_001956"
     CDS             complement(4917..5219)
                     /gene="gatC"
                     /locus_tag="VW528_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9912726.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            5432..6976
                     /locus_tag="VW528_001957"
     CDS             5432..6976
                     /locus_tag="VW528_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9912727.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            7231..7428
                     /locus_tag="VW528_001958"
     CDS             7231..7428
                     /locus_tag="VW528_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9912728.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            7539..7715
                     /locus_tag="VW528_001959"
     CDS             7539..7715
                     /locus_tag="VW528_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9912729.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
BASE COUNT         2175 a         1531 c         1762 g         2273 t
ORIGIN      
        1 cgttactgga aactcatcca acaggatagt cgtaaattga gcgataaacg tttttatcgc
       61 cctacttttc gcatgcactt aacaaataaa gaaattcttg acaagatttt aagctattca
      121 gaagacttga aacaccacta tcagatctat caactcttac tttttcactt tcagaataag
      181 gaaccggaga aatttttcgg actcattgag gacaatctga agcaggttca tcctattttt
      241 cagactgtct ttaaaacctt cctcaaagat aaagaaaaga ttgtcaacgc ccttcaacta
      301 cactattcta atgccaaact ggaagcgacc aataatctca tcaaacttat caagcgcaat
      361 gcctttggtt ttcggaactt tgaaaacttc aaaaaacgga tttttatcgc tttgaacatc
      421 aaaaaagaaa ggacgaaatt tgtcctttct cgagcttagc ttttcttcaa cccactacag
      481 ttgacaaaga gccttattgt tcttctgtta caaactggct gagcagtcca ttgataaaac
      541 gggcagattt ttgatcggag aagtcctttg caagctcgat agcttcatta acagcaacca
      601 gctgaggagt gtcaaatgaa gtgatttcaa agactcccaa gcgaaggagg tttctctcca
      661 cgagcgttaa gcgttcaatg gtccaacctg cttttaaatg ctgagtgatt tgcttatcta
      721 gttcttcctt tttagcttga acaccagaaa cgaggtctat caaaaaggct ggaagttgta
      781 catccgtata ttcacgatca tgagtatagg cgaaacgaca agcagtttcg acatccgtac
      841 cgaactcaag gctcatgaga gcttgaaaag cgcatttacg gagttggcgt ctagattcta
      901 atagtggact agtcattgag gaagtcctcg tcaaatagat ctttcaattc tggttttggt
      961 gttttatctg ggacgatacc tgcaacgtga atattgatag cagcgagttc tacatcagcc
     1021 atattacgga cggcatcttt gacagctttc tggatagcaa ccgctacctt aggaactttt
     1081 actccgtact caaggtagag atagatatct gctgtgagtt cttcgtccac gtttttaaga
     1141 taaatgccac ggccgagtga aagttttgaa agggtatcag acactgatct gtttgaaaaa
     1201 gagtgaacac cctctacctt tgcagtagcg atagcaatga ttttttcaag tacacgtggg
     1261 gcgataacga tttcgccaag ttgttcttca attcccatag aatgacctct ttctagagat
     1321 taggcacgag aaacgtaagt tccttctgca gtgttgataa cgagtttttg tcctgcttcg
     1381 atgaagtctg gaacgtttac gacaagtcca gtttccatcg ttgctggttt accagaacct
     1441 gtaacagtag cacctttgat agatggttga gtttcagcaa ctgtcaactc aacagtagta
     1501 ggaacggtga caccgatcac ttcagttccg tagaattgga ttttcacatc agagttttca
     1561 aggatgtaaa gcaattcgtt ttcaacattg actacaggga tttcgtattg gtcgtaagtt
     1621 tctgtattca tgaagtatgc tgtgtcatcc attttgtaca agtattgagc tgggacagtc
     1681 tcgataatag cttgttcaaa tttttcctct ggacggtagc ttgtgtcaaa tgtagaacca
     1741 gtacggacat cacgcaattt catacgcatg atcgtgtttc ctttacctgg tttgtggtga
     1801 ctagcttcca aaacgcgaat caatttgccg tcagctgttt caaaggtcat accagctttt
     1861 aatttacttg cttcaatcat gtttttacct ctttaataaa tattattact cttcatttta
     1921 ccataaaata ctctggaaat aaagccaaaa tagttattgg gagttggtag agaagaaaaa
     1981 accaacctta gggctggttt tgttctgtct agttttcttt caacttcgcc aattcctgtg
     2041 caagtagttt aagggcaact tgtgggttgg cttggccttt ggttgccttc ataaggaatc
     2101 ctgtaaaggc cttgtcggcg ttacgtttgc ctgacttgaa gtcggcaaca gcagcttcgt
     2161 tatcggcaaa gacttggtgg atgattggga tcaagatagc tggatctgaa atttgaacca
     2221 tacctgcttt ttccacgtat tcacgcgcgc caccgccatt tttagctaga tggacaaaga
     2281 ctttcttggc aatcttagat gaaatagtac cgtcttcgat gatggtaatc atttcaacca
     2341 agttttctgg tgtcaattcg atttgttcca gtgttttacc ttcagcattc aagaactgag
     2401 cgacttcccc ttggagccag ttagagactt gtttggcatc accacctagg gcaacagctt
     2461 tttcaaagaa gtcagaagtg actttattag cagtcaactg actagcatcg tagtctgata
     2521 aaccaaggtc agatacataa cgcgcacgac gttcttttgg aaactctggc aactcagtcc
     2581 gcatttcctc aatccactcg tcagaaattt caaagagggg taggtctggt tctgggaagt
     2641 agcggtagtc agcagcccct tccttgacac gcatgaggat ggttgttttg ttagcttcat
     2701 cgtaacggcg tgtttcttgg cggatttgac cacctgagcg aagaatttca gcctggcgtt
     2761 ggacttcgta ttcaagacct ttacgaacgt ttgagaagga gttgaggttc ttcaattcag
     2821 tcttggtacc gaatttttct tgaccataag gacgaaggga gatgttggca tccacacgca
     2881 tagaaccttc ttccatctta acgtcagaaa tgccagcgta ctggataact tccttgaggg
     2941 ctgtcagata agcataggct tcttcaggag aacgcatatc tgcctcagat acaatctcaa
     3001 tcaagggaac cccttggcgg ttgaggtcaa cataagagta gccatctgta ccatgggtgt
     3061 ttttaccagc gtcttcctct aagtgggcac gttcgatacc gattttcttg gtcgtaccgt
     3121 cttctagttt gacttcaatc cagccattat atccgattgg ttcatcaaat tgagaaattt
     3181 ggtaggcttt ggggttatca ggatagaagt agttcttgcg gtcaaagtgc atctttttgt
     3241 ggatgtccat gttgagggca agagcagcct tgataccggc atcaacaacc cctttattga
     3301 gaactggtag aactcctggg aaagaccagt caattacgtt agtgttggca ttttggtcat
     3361 ttccgaagtg ggcagaagta ggtgagaaga tttttgaatt ggtgttgagc tctacgtgga
     3421 cttcaagtcc gatgactgtt tcaaagttca ttagttgtca cctccaaaaa tcacgggttg
     3481 ttgtttgtgg tagtctgttg ttgcttcaaa agcagcagca gcttggtaaa tggtttcctc
     3541 agagtacttg ggaccaatca attggagtcc gacaggtaga ccttgagaga atccagcagg
     3601 aatcgaaatt ccaggcagtc ctgccaagtt tacaggtatg gtcaagaggt cggctaagta
     3661 catggcaact gggtcatggt tgagagaatc caagtcatag gcaacacttg gagcagttgg
     3721 acccaaaatc aaatcgtaat ccgcgaagac tttttcgaaa tcttgaatga taagtgtacg
     3781 gacctgtcca gccttcttgt agtaggcatc gtagtaacct gatgaaagac tgaaagtacc
     3841 cagcatgata cgacgtttta cctcttcacc aaaaccttgg cttcggctgt ttacatagat
     3901 ttcatcaagg ttggttgcat cttctgcgcg atagccgtaa cggataccgt caaagcgttg
     3961 caagtttgat gaagcttctg atgaagcgat gatgtagtat acggcaactc cgtatttaga
     4021 gtgaggaagg ctgacttctt cgacaatagc accaagtttt tcaaagtgtt tagcggcact
     4081 tagaatggtt tccttaacct ctgggttaat tccttcaccg aggtattcct taggcaaagc
     4141 gattttcata cccttgatgt cttggccgat ttttgaagta aagtcggcga tgcggacaga
     4201 agcagaagta gagtctttag catcttcgct ggcaatagcg ttgagcaaga gggcattttc
     4261 cttaacagta ggagcaaaag gtccaatctg gtctaatgag ctgccaaagg caatgagacc
     4321 gaaacgtgaa actgttccgt aggttggttt gagaccaacg attccgttga aggcagcagg
     4381 ttggcggatg gaaccaccag tatcagaacc aagtgacaag cgaacttgtc ctgaggctac
     4441 agctgcggca gaaccacttg atgacccacc aggaaccttg ctgtggtccc aagcgttttt
     4501 agttgctccg tagtgtgaag tttcacctga accacccata gcaaattcgt ccatgttggt
     4561 ctttccaacg acaatcatgc ccttggtttt tgcattggca acagctgtcg catcaaagat
     4621 tggctcatag ttgtagagca tttttgaggc agcagttgtg agaataccgt ctgtagagat
     4681 gttatcctta acagcaagtg gaattcctga aaggacattg tcagtatcaa ttccagcttc
     4741 atcaatggct ttagcttgaa caagagcttg ctcctcagcg atggtgacaa atgaattgag
     4801 ggcttcctca cgagacttga tattttcaag tgttgcttgg gtcaattctg ttgcagaaat
     4861 ttccttagag acaaggagat tgtgcaactc ttcaatagtt ttatcgttaa aagtcattag
     4921 gcatctcctc catcgtctag gatagctggc accttgatat agtagttgtc tttttcaggt
     4981 acgtttttaa acaagcgatc acggtctgtt ccttcttcgg ccacatcagg gcggagtaca
     5041 gtcttgcggt cagccatagt cgtagtaggt gcgacaccag ttgtgtcaac ttcgcccagc
     5101 aattcaacca tgtcaacaat cttagacaag gtggtcgcaa aggcagcagt ttcttcttca
     5161 gagaatctta attttgaaag attggcaacg tgtgttacct cttcttgcgt aattttcatc
     5221 ttgcatcctt tcgtgaaatg atgattttta gtctgttcta ttttaccata ttttcctata
     5281 aataagggag ggggaagtga aaagaaatag aaattgaaaa aaatgctaaa atccgatata
     5341 atggagtgtt gaaaggaatg gtaaaatcca atgtaaaaat cattctcagc tattgaaaca
     5401 agaaaaatag agaatcaaag aaagagaact tatgaatatt caagaagaaa ttaagaaacg
     5461 tcgtaccttt gccattatct cccacccgga cgcggggaaa acaaccatca ctgagcagtt
     5521 actctacttt gggggtgaga ttcgtgaggc tggtacggta aaagggaaga aaacagggac
     5581 ttttgctaaa tctgactgga tggatatcga gaagcaacgt gggatttctg ttacttcatc
     5641 tgttatgcaa tttgactacg acggcaagcg cgtgaatatc ttagacacgc cagggcacga
     5701 ggacttctca gaagatacct atcgtacctt gatggcggtg gatgctgcgg tcatggtcgt
     5761 ggactctgcc aaggggatcg aggctcaaac caagaaattg tttgaggttg tgaaacatcg
     5821 tggcattcca gtctttacct ttatgaacaa gctggaccgt gacggtcgtg agcctttgga
     5881 tctcttgcaa gaattggaag aaatcttggg cattgctagc taccctatga actggcctat
     5941 cgggatgggg aaagcctttg agggcttgta tgacctctat aaccaacgtt tagagcttta
     6001 taaaggggat gagcgttttg ccagtctaga agacggggac aaactctttg gtagcaatcc
     6061 tttctacgag caagtcaagg atgacattga gcttttaaat gaagctggga atgagttttc
     6121 agaggaagct attctggctg gagaattgac gcctgtcttt ttcggttcag ccctgacaaa
     6181 ctttggtgtg cagaccttcc ttgaaatctt cctcaagttt gctccagaac cacatggtca
     6241 caagaaaaca gacggtgaaa ttgtggatcc ttatgacaag gatttctcag gctttgtctt
     6301 taaaatccaa gccaacatgg atcctcgtca ccgtgaccgt attgcctttg tccgtatcgt
     6361 atcaggcgaa tttgagcgcg gcatgagtgt caatctccct cgtactggta agggggctaa
     6421 actatctaat gttacccagt ttatggcgga gagtcgtgag aatgtgacca atgccgtggc
     6481 aggtgatatt atcggggttt acgataccgg tacttatcag gttggggata ccttgacggt
     6541 tggaaaaaac aagtttgaat ttgaaccact gccaaccttt actcctgaaa ttttcatgaa
     6601 agtttctgct aagaatgtta tgaagcaaaa atccttccac aaggggattg agcaattggt
     6661 gcaagaagga gccgttcagc tttataagaa ttaccaaaca ggtgagtaca tgctgggagc
     6721 tgttggtcaa ctccagtttg aagtctttaa acaccgtatg gaaggcgaat acaatgcgga
     6781 agtggtcatg agcccaatgg gtaaaaagac cgttcgttgg atcaagcctg aggacttgga
     6841 tgaacggatg tcgtcaagtc gcaatatctt agccaaagac cgttttgacc aaccagtctt
     6901 tctctttgaa aatgactttg ccctccgctg gtttgcggac aagtatccag acgtagagtt
     6961 agaggagaag atgtgactca gtaccaacaa ttggaactaa agttccaaat tgttggacgc
     7021 tagtcgctat ttggcgaact agctaactgc ctcactaact acgtttggca aatgaaatcg
     7081 atttgtaaaa ctccgtgtcg taatgtaaaa attgtaaacc gtaatgggac ttcatctaaa
     7141 atctccactg gtatggtttt acgaacgaat aggcgaaaat aatggttacg tagaaacata
     7201 tgatgggaat aaaaagaaat gagataaaaa gtgaaagaag agaaaaaagc gattgtctta
     7261 ggtgcagata atgcttatat ggataaagtt gaaacaacaa taaaatctct ttgtgttcat
     7321 cattataatc ttaagtttta tgtttttaat gatgaccttc cgcgagaatg gttccaattg
     7381 atggaaaaaa gactagagac tttgaatagt gagattgtga atgtttagat tgacagtaat
     7441 attctgaaag gttatcgtct cccctttgag ggactttcat atgcggcttt tttaggtatt
     7501 ttattccaaa gtatgtaagc gagtctcgtg tactgtattt ggattcagat attgttgtaa
     7561 gaaaatcgat agatgagctg tgggatttgg acttgacggc tataccctta gctgcagtta
     7621 gagatgattt ttatacacat aattttaatt ctggtgttct gttaatcaac gatggcatgt
     7681 ggagagcaga aaatgtcacg caagacctga tttaagacac aaataaaaaa tcttccaatt
     7741 c
//
LOCUS       DAVKQB010000075         7645 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKQB010000075 DAVKQB010000000
VERSION     DAVKQB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.075"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(1..846)
                     /locus_tag="VW528_001960"
     CDS             complement(1..846)
                     /locus_tag="VW528_001960"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9912730.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKACAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            1102..2129
                     /locus_tag="VW528_001961"
                     /pseudo
     CDS             1102..2129
                     /locus_tag="VW528_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2430..4319)
                     /locus_tag="VW528_001962"
     CDS             complement(2430..4319)
                     /locus_tag="VW528_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9912731.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGTEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKEQSKQLLTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFERDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLLGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            4457..6022
                     /locus_tag="VW528_001963"
     CDS             4457..6022
                     /locus_tag="VW528_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9912732.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYANAKSLDDFNGAKITSQQGVYLYD
                     LIAQITGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMVQVEPGF
                     KTGEEDTAIAIGLRKDDTRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTITGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWILNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            6022..6762
                     /locus_tag="VW528_001964"
     CDS             6022..6762
                     /locus_tag="VW528_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9912733.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(6871..7086)
                     /locus_tag="VW528_001965"
     CDS             complement(6871..7086)
                     /locus_tag="VW528_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912734.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         2417 a         1703 c         1355 g         2170 t
ORIGIN      
        1 ttatacaaat aaacggacaa aactgtaaag tagcaaaaca ctaccaagca cgatacggta
       61 tttaccaaaa agggtgaagt cgtgtttttt cacatagctg gtcaagaagc gaatagccac
      121 catgctgacc gcaaaagcta ctcccatcgc gaccaagagc aaaaacaatt gcccaaagct
      181 caagagttct ccggctttca caaatttgaa aatctttaag gcactagctc caaacataac
      241 gggaatccca agatagaagg taaattctgt cacaactgaa cgactggttc catttaacaa
      301 accaccgaca atcgttgcac ctgaacggct agtccctggt aaaagagcaa gaacttggaa
      361 gagtccgata tagaaagcgg tcgtataagg aagcttgtcc aactctgtta cacttggctc
      421 gatagcacac gctttattgc gcttttccaa atagatgaag gcaaccccgt agataatcaa
      481 catgagagca actgaaacca tgttatggaa gtgggtatca aaccaatcat caaatttaaa
      541 gacaccaagt aaaggtaaag tagcaatcaa gaccttcaac catagtctcc aagtcttacg
      601 aacttcctgt ttgtccttag tcggtttaaa aggattgagc ttgttaaaat aaatcaccat
      661 aactgctaaa atagcaccaa gctgaatcac gacattaaac atggacataa aggcttcatt
      721 ttgattttgg tattggataa actcctctgc taaaatcaag tgacctgtac tggaaatcgg
      781 caaccattcc gtaattcctt caacaatccc gaagaagata gattttaaaa tttcaataag
      841 atacatatat ttctccttat attatattta acgcagttag ctactgccaa agcccaagtg
      901 gtatacttgg aataagccac tgtggattag ttcatttcct ccaatcgctt gacgatttcc
      961 gaaaggatct aaccacagct ttttgtttca attggtaatg agtttgataa cgcatagacg
     1021 ttttatctcg tctaaggtaa caaggcaaaa agttcgtgga taaccaagta gacttgaaat
     1081 actatatctg gaggtactcc catgctttat gtgggcattg atgtcgctaa aaataaacac
     1141 gatgttacag ccttgaatgt tcgaggaaaa ctgttcttaa accactcact ttttcaaata
     1201 ataaagctgg ttttgaacta ttagatctgt ctcttcgaca gctcaaccaa gactgtctca
     1261 tcgctcttga agataccgga cattatgcct ttaatctact gaacttcctg catgaacaag
     1321 gatataaagt ttacacctac actcctttgc tcatcaagga atttgccaaa tcattatcac
     1381 ttcgcaagac taaaacggat aagaaagacg ctcatggcat cgctcttaaa cttctttctg
     1441 accccaatcg tgaacaattt caacacgata atcggcaagt agaactaaaa atactggcta
     1501 gacatattca tcgtctcaag aaaaaacagt ctgattggaa agtacaatac actcgttgtc
     1561 ttgatatcat ctttcctgag ttggataaaa tcgttggaaa gcattcagaa tatacctacc
     1621 aactcttgac atgctaccct aatcctcaga aaaggcttga ggcaggattt gataagctga
     1681 tagaaattaa gcgattgacc gcttctaaaa ttcaggatat cctctcagtc gcacctcgtt
     1741 ctatcggaac aacatctcct gctcgtgaat tcgaaatcat cgaaaacatc aaacattaca
     1801 agaggctcat tgacaaggcg gaaaaatgtg tcaatgactt gatggctgag ttcaactcgg
     1861 tcatcacgac ggttactggg attgggaatc gtttaggggc ggtcatttta gccgagattc
     1921 aaaatattca tgcctttgat aatcctgctc aattacaagc tttcgctgga ctggattctt
     1981 ctatttatca gtcaggtcag attgatttag ctggaagaat ggtcaaacgg ggttctcctc
     2041 atctgcggtg ggcactcata caagctgcca aagcatgcgc tcgcttttca cctgctttta
     2101 aggcctatct taagactaag ttagaataag aaaaacatta caatgtagcc atcatccacc
     2161 ttgcaaaaaa acttatccga accctgtttt atatcctaaa aaagagctgc catttgacga
     2221 acaaaaagtg atgtaatttc tgaaatcaac acaggattac ctttgaaaaa atggactttt
     2281 tcaagggctt tttgccgtgc tctaaaatca tcgccaaatc atcaaattga aataagaaat
     2341 agtttagata aaattattga cttttcttat agtttgtctt tcttctatct tccattatat
     2401 catacttttt cagtctatgg tagctctctt taaaaggctc cgatactgcc gccacctccg
     2461 cctccagaga agccaccgcc agaacttcca cttccagaag atacggagta ggtacttgct
     2521 gtatttgcga cactagcata atggctcatt tgcgcgcttg aatgataaaa catactgtgc
     2581 cagccataag ctacatagag attgatatct ggattttcaa cttgaatctg atgaaccttc
     2641 atcaaatgac taaccttgtc cgcatagcca agtaaggttg catagaccaa gagacgattc
     2701 caaacaacaa tactttccaa ttcagcctta tccagtcgtg caatctcacg taacatattt
     2761 tcaaaactgg tccagagata gtagacctca gctcccgctt catttagaac accatcacga
     2821 ttatctagtc gaagcttcca ataatagaaa acagacaaaa ctaaccctag aaaaccaagt
     2881 attggcaaag ggaggtaaag atagccatga acgtctaaac tgtacaagaa caaaccaaat
     2941 ccgataaata ggggcaggat agtcaaggca cccataccca cttgcaaggc cttttcccca
     3001 ccagttaaag gacgataata atctgggagc ccccagaagg aaactcgttt tctcactcct
     3061 tcttgcatct ggttcaatac ctcttcaaaa gaagatttga gttgaagccc tcttgcttga
     3121 atccgttttt catcagaaac tttggctcta cgataaagac tatcagatac cttgtaatcc
     3181 gcaaacaaat tggaaagagt ttcttctttt ttacctgaaa aggctagatt tagacagtct
     3241 ctctcaaagc ttgacaaacc atcttctttt actagcctca aaccaactgc atctccttct
     3301 gaaatgatag agacattccc acggtctatc acatctagca aggtagcttg aataagttga
     3361 tcaaaggtga attttccagc tcccttgacc aagggactca cttcctccaa ggaggtcgag
     3421 tagactgctt ctgataaaac cataggctct aattccattg gtggttcata gagacgatga
     3481 tttttggcat atttgactga aggagtggtc tttcttctat aaataaaata gaagcagaca
     3541 ctcaataaca aggagatgga aaggatcgaa gggaggaccc aagtaagtag ttgtttactt
     3601 tgctcttttt ctctaacaat cgagtcttct atcttattaa actcttctaa tcgattccct
     3661 ttcaatccct gatccctagc gctagcaaaa tcggtccgag gccaataggc atgcaactca
     3721 actccacgct tagccggaag attgtctaaa cggatagtat aatcaaggtt actcttttca
     3781 atcgttccct ctctaaaaag tttccctgta tggaaaaaga gtttttcagt ccccttgtct
     3841 ccccttacat gaaattcaaa cttttcaata gactctgaac tatctgtcag aggttgccaa
     3901 tttaattcag cgatatcatc ataaaggaaa agtaaatttt ttaagttcca gacgaggtca
     3961 acttcaacta tgtcgccctc ctgacctgga ttatagactc tcacagtata accatccgct
     4021 tcttctgtta cttcgctagt cacatctgct agttctgcac cgtttttcgc ggcctgaatc
     4081 tttggatgag ggtcaatgtc aaacccgcta ggcatcttac cagcacgtcc aagtcccacg
     4141 atttggccct taaagtcctc ctcaaactgg taaactatct tctgtctaaa ctctgccgta
     4201 ttatctgcat gaatatacaa atccccttga taagagttta tcttgaaatc aatggcaaaa
     4261 acagagagtg gaagaaggca aaacaagcct aacaccagta agaaaaaagt ttttttcatc
     4321 aactaagccc cctttttatc tttcttatca ttatagcatt ttttcgtaag taaaggctta
     4381 cttatgttga aaaattacgc atttttcgat aaaataggag acagttattt tttaatagat
     4441 cagaatagga gaaatcatga gaaaaatata cttatctatt ttcacaagtc tcttgctgat
     4501 gctaggactt gtcaatgttg ctcaagccga tgaatattta cgcatcggga tggaagcagc
     4561 atatgctccc tttaactgga cccaggatga tgatagcaac ggagctgtca aaatcgatgg
     4621 gaccaatcag tatgccaacg gatacgatgt tcaaatcgcc aagaaaatcg ctaaggactt
     4681 aggtaaagaa cctttggttg ttaaaaccaa gtgggaaggt ctagtccctg cccttacttc
     4741 tggtaagatt gacatgatta tcgcaggtat gagtccaact gcagaacgca aacaagaaat
     4801 tgccttttcg agcagttact acactagcga accagttcta ctggtcaaaa aagattctgc
     4861 ctatgcaaat gctaaatctt tggatgactt taatggtgcg aaaatcactt ctcaacaagg
     4921 tgtctacctt tatgacttga ttgcacaaat cacaggtgct aaaaaagaaa cagccatggg
     4981 agacttcgct caaatgcgcc aagctcttga ggctggtgtc atcgacgcct atgtttctga
     5041 acgcccagaa gctctgactg ccgaagccgc taactctaag ttcaagatgg tccaagtaga
     5101 accaggtttc aaaactgggg aagaagatac agctatcgcc attggacttc gtaaagatga
     5161 cactcgtatt agccaaatca atgccagcat tgaaaccatt tcaaaggacg accaagttgc
     5221 cctgatggat cgtatgatca aggaacaacc tgccgaagct acaacaactg aagagactag
     5281 cagtagtttc tttagccaag ttgctaaaat tctttctgaa aactggcaac aactcttgcg
     5341 tggtgctggt atcactcttt taatctctat cgtcggaacc atcacaggtc tcattattgg
     5401 acttgccatt ggtgttttcc gtactgctcc tctctctgaa aacaaagcca tttacggcct
     5461 acaaaaacta gtcggctgga ttctcaacgt ctacattgaa attttccgtg gtacgccaat
     5521 gattgttcaa tcgatggtta tctactatgg aactgcccaa gctttcggga tcaaccttga
     5581 ccgtacactg gctgctatct tcatcgtttc aatcaatacc ggtgcctaca tgactgaaat
     5641 cgtccgtggt ggtatcctag cagttgacaa gggacaattt gaagctgcga ctgctcttgg
     5701 tatgacccat aaccagacca tgcgtaagat tgtcctacct caggtagtcc gtaacatcct
     5761 acctgcaact ggtaatgaat ttgtcatcaa tatcaaagat acatctgtat tgaacgttat
     5821 ctctgttgtc gaactttatt tctcaggaaa taccgtggca acacaaacct atcaatactt
     5881 ccagacattt acaatcatcg ccgtgattta ctttgtcctc accttcaccg taacacgtat
     5941 cctacgattt atcgagcgcc gaatggacat ggatacctat actacaggtg ctaaccaaat
     6001 gcaaacggag gatttgaaat aatgacacaa gcaatccttg aaattaaaca cctcaaaaaa
     6061 tcctatggac aaaacgaagt gctaaaagac atttcactca ctgtccacaa gggagaggtc
     6121 atctctatca tcggaagctc tggaagcgga aaatcgacct tcctacgctc cattaaccta
     6181 cttgaaacac caactgatgg acaaatcctt tatcatggac aaaacgtcct cgaaaaaggc
     6241 tatgacctca cgcaataccg tgaaaagttg gggatggttt tccaatcctt taacctcttt
     6301 gaaaatctca atgttcttga aaacacaatc gtcgctcaga caactgtcct aaaacgcgaa
     6361 cgcacagaag ctgaaaagat tgccaaagaa aacctggaaa aggtcggcat gggagaacgc
     6421 tactggcaag ccaaaccaaa acaactctca ggtggtcaaa aacaacgtgt ggccatcgct
     6481 cgtgccctct ccatgaatcc ggacgctatt ctctttgatg aaccaacatc agctctcgat
     6541 ccagaaatgg ttggagaagt cctcaaaatc atgcaggacc tggctcagga aggcttgacc
     6601 atgattgtcg taacccatga aatggaattt gcccgtgatg tctctcaccg tgttatcttt
     6661 atggataagg gcgtgatcgc tgaagaaggt aaaccagaag acctcttcac caatcctaaa
     6721 gaagaccgaa caaaagagtt ccttcaacgc tatctcaaat aaaaagaaaa ggctgcatca
     6781 accgtgcagt ctttttgctg tcctcaaaat gaaaaggcag attctctatc tctactactt
     6841 gaaggtcaag aatccgccgt tatccaaaga ttaatcttca aatttttcat aatttttttc
     6901 atagaaatca attaggccta gggctgtttc aaatgaaata ttttttactt ttgcacgacc
     6961 ctgcgccaga gcaatgatag acatttcacg cgcattcgtt tctttagaga tacggtagcc
     7021 tgtgattttc ttatcacgaa cccagccaac aactgattct actttttcaa agtttgactt
     7081 agccatgttt ttctcctatt ttcttcttta taatatttaa ttgtatacgt ttttactact
     7141 tttgtcaata aaaaaacatg tattcaacga aaataattag ttcagaattt tagcttcaga
     7201 attttcttac ttcctttttg aatccgataa tatataggtt ttactagtct ctacaccata
     7261 aatatttgct aataaaaata ctgtattatt accctcttaa ggtgcgaaat tattcaagtt
     7321 cttaaaaaat gcttctctat ttccgtaaga tagcttattt cttcgattta agaactgaag
     7381 atgatagttc taacctttag aaatccaagc cttcactagc tcaaaaactc ctatctttct
     7441 tcctcaataa atcatctgag agataggtat tccttatgtc cttcaaattc atcactcctt
     7501 tggatattga ggtgctagcc tagaagcaga cacccgatta tctaaaccat ccttaataaa
     7561 gatgacaaga cagataagct cagttaatta cttatatatc tttttaatat ggtatatttg
     7621 tcaacaaaaa gaaatcaaat catac
//
LOCUS       DAVKQB010000076         7118 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKQB010000076 DAVKQB010000000
VERSION     DAVKQB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.076"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..449
                     /locus_tag="VW528_001966"
     CDS             <1..449
                     /locus_tag="VW528_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912735.1"
                     /translation="VILDKNYQNKARKSIEIEEWKKLRGCVEIKSLKHDLITEEKIKS
                     TISNSFEHITGEMGQLIGDLLWYKKVIYTNGIEWRFLSLDDKEEIDNTIVQVVNKRIE
                     TEEAGNSFDWWKNIKDLSFNYTDIYLSKDCIQEWDEFVKKVKEIEW"
     gene            488..589
                     /locus_tag="VW528_001967"
                     /pseudo
     CDS             488..589
                     /locus_tag="VW528_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
     gene            complement(640..846)
                     /locus_tag="VW528_001968"
     CDS             complement(640..846)
                     /locus_tag="VW528_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9912736.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDILEAVKNCPTRALIGNQEA"
     gene            898..1374
                     /locus_tag="VW528_001969"
     CDS             898..1374
                     /locus_tag="VW528_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9912737.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKGFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMVTGSWFANPGDVIKIK"
     gene            1383..2054
                     /locus_tag="VW528_001970"
     CDS             1383..2054
                     /locus_tag="VW528_001970"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9912738.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            2185..2523
                     /locus_tag="VW528_001971"
     CDS             2185..2523
                     /locus_tag="VW528_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9912739.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            2526..3155
                     /locus_tag="VW528_001972"
     CDS             2526..3155
                     /locus_tag="VW528_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9912740.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            3395..4546
                     /locus_tag="VW528_001973"
     CDS             3395..4546
                     /locus_tag="VW528_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9912741.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            4859..5608
                     /gene="truA"
                     /locus_tag="VW528_001974"
     CDS             4859..5608
                     /gene="truA"
                     /locus_tag="VW528_001974"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9912742.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            5598..6371
                     /locus_tag="VW528_001975"
     CDS             5598..6371
                     /locus_tag="VW528_001975"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9912743.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            6349..6810
                     /locus_tag="VW528_001976"
     CDS             6349..6810
                     /locus_tag="VW528_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9912744.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     AIIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            7037..>7118
                     /locus_tag="VW528_001977"
     CDS             7037..>7118
                     /locus_tag="VW528_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9912745.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNK"
BASE COUNT         2098 a         1254 c         1571 g         2195 t
ORIGIN      
        1 tagttatttt ggataagaat tatcaaaata aagccagaaa aagtatagaa attgaagaat
       61 ggaagaaact tcgaggatgc gtggaaatta agagtttaaa gcatgattta attacagagg
      121 aaaaaatcaa atcaaccata tcgaacagtt ttgaacatat aactggagag atgggacaac
      181 ttattggaga ccttttatgg tataaaaaag taatttatac aaatggaatt gaatggagat
      241 ttctaagttt agatgacaag gaagagatag ataatacaat tgttcaagta gttaacaaaa
      301 gaatagaaac agaagaagcg ggaaattctt ttgattggtg gaagaatatt aaagatttat
      361 cgttcaatta tacggatata tatttatcta aagattgtat acaagagtgg gatgaatttg
      421 taaagaaagt aaaggaaata gaatggtagg agtctattct cattgagaaa tttagattat
      481 aagtcgcagt aagatttatt tagagactaa aaaaagacca ctttatctta tcaaagaaaa
      541 aagtgacctt cctgatttta cagaaaaaaa taaagtgaaa agactataat tttacatgga
      601 aatgtgctaa actagaggga gtggattatc gccattgatt taggcttcct ggtttccaat
      661 cagggcgcga gttgggcaat ttttaacagc ctctaagata tcctgactag gagaaatttc
      721 tttttccagt tggtcagggt catcgtaaaa acgcacgatt ccattatcgt ggtaatcaaa
      781 taaatcagaa taagtttggc aaagcccaca ggcgatacat cgttcaggta taagtgtgat
      841 tttcatgttc atatttatat tgtaataaga aaagtaaaaa aaaacaagga gtaaggtatg
      901 gcaaaagaac cgtggcaaga agatatctat gatcaagaag aatcaagagc agagcgtcgg
      961 catcgaaacc acggaggggc tgataggatg gctaatcgta ttttgacgat cctagctagt
     1021 attttctttg taattgtggt ggtgatggtc atcgttctca tctatctatc atcggggggg
     1081 agtaatcgca cagcagcctt aaaaggcttt catgattctg atgcaagtgt agtacaaatc
     1141 tcatcttcaa gcagttctca gcctgagcag agttcagagc cagaatctac ttctagtagt
     1201 tcagaagaag ctgctaatcc tgaaggaacg attaaagttc tcgcaggaga aggggaagca
     1261 gctattgccg ctcgtgcagg aatctccatt gctcagttag aggccttgaa tcctgggcac
     1321 atggttacag gatcttggtt tgctaatcca ggtgatgtta taaaaataaa ataggagccg
     1381 atatgaaaac aattcaaatt gctattgatg gtcctgcttc cagcggtaag agtacggtcg
     1441 caaagattat tgctaaggat tttggattca cctaccttga tacaggagct atgtatcgtg
     1501 cagcgaccta tatggctctt aagaaccaat taggagttga agaagtcgaa gcccttctag
     1561 ccttgttgga ccagcatcca atcagctttg gacgttcaga aactggagac cagcttgttt
     1621 ttgtaggaga tgtggatatt acccatccta tccgtgaaaa tgaagtgacc aatcatgttt
     1681 ctgctattgc agcaattcct gaagtgcgtg agaaactggt ttctctccaa caagaaattg
     1741 cccagcaagg cgggattgtc atggatggtc gcgatattgg aactgttgta ttgccacaag
     1801 cagaattgaa aattttccta gtagcttctg ttgatgagag agcagagcgt cgttacaagg
     1861 aaaatattgc caagggaatt gaaacagacc ttgaaaccct aaaaaaggaa attgctgcgc
     1921 gtgactacaa ggatagtcat cgtgagactt ctcctctcaa acaagcagag gatgctgtct
     1981 accttgatac aactggtttg aacattcaag aagtagttga aaaaatcaaa gcagaagctg
     2041 aaaaaagaat gtaaatgtag aaaagccgat agattgatat cggttttttt ctaaatttgt
     2101 caaaatgctg attttagggt ataatagttg ctgttcgaga aattttgatt ttcaaagaat
     2161 agtgaatgat gataaggaga aaccatgaac aatttaccaa attgtccaaa atgtaactca
     2221 gagtatgtct acgaagacgg tgccctactg gtttgcccag agtgtgctca tgagtggaat
     2281 cctgctgaag ttgcagaagt agaagaaggt cttgtcgcta tcgatgccaa cggaaataaa
     2341 ttgactgatg gtgatacagt aactctcatc aaggacttga aagtaaaagg tgcgccaaaa
     2401 gatttgaaac aagggacgcg cgtgaaaaat atccgcatcg tagaaggcga ccacaatatc
     2461 gactgtaaga ttgatggttt tggtgccatg aaacttaaat cagagtttgt gaggaagatt
     2521 taagcatgtc aaaacactat aaacttgtat tttatagccg tatcttcttg tttctagcgg
     2581 cttttacggg agtttatctt gaaattacta agcatggtgg ttttgggatg cttctctatt
     2641 acacggttct gtccaacctc ttggtaacta tttttaccct ttatcttcta aaggttatga
     2701 gccgtgtagg tgaaaattgg caaaggccaa gtctcttgcg cttaaaaggt ggggtcacca
     2761 tgagtatcat gattacctgt gtgatttacc atttcctctt ggcgcccatt gcgactaatt
     2821 tctataccct agaaaatttc ctttgccact atatcgttcc catctggttt ttagcggata
     2881 ccctcttttt tgacaaacag ggtcaataca agatttggga tccagcagtg tggaccattt
     2941 taccctttct gtatatgatg tttgctcttt ttaatggctt ggttctaaaa ctcaatattc
     3001 caaatgctaa ggataatcct ttcccttact tctttttgaa tgtgaacaag ggttggaatg
     3061 ttgtgtttaa gtggtgtctg attatctttg ttgcctatat ggtagcagga tttattttct
     3121 actttatcaa gcaaatcaag agaaagtcat cctaatactc ttcgaaaatc tcttcaaacc
     3181 acgtcagctt ccatctgcaa cctcaaaaca ctgttttgaa caacctgcgg ctagcttcct
     3241 agtttgctct ttgattttca ttgagtataa gatacaaggg attcattttc gaatttaaga
     3301 aggagtctac aaaccaatga ggcttcttct tttcttgctt atttgataac tatcaaaaaa
     3361 gtagaagatt aagagaaata tagaaagaga tttcatgaaa caatttttag aacgggccag
     3421 cattttggct ctctccctcg ttttgattac ctccttttcc atttcgagtg ccctaccagc
     3481 catgtttgac tattatcagg gttattctaa ggaacaaatt gagctcttgg tgagcttgcc
     3541 ttcctttgga atcatgatga tgttactgct aaatggtttc ttagaaaaaa tatttcctga
     3601 gcgcttacag attagtttgg gcttgctgat tttatcattg agcggtacag ctcccttctg
     3661 gtaccaagcc tatccctttg tctttggaac acggcttctc tttggtttgg gtcttgggat
     3721 gatcaatgcc aaggccattt ctattatcag tgaacgctac caaggaaaaa ggcgaattca
     3781 gatgttaggg ctacgcgctt ctgcagaggt cgttggagct tctctcatta ccttggccgt
     3841 cggtcagttg ttggcctttg gttggacagc tatctttcta gcctatagtg ctggattttt
     3901 ggtgctgccc ctttatctgc tctttgtccc ttatggaaaa tcaaagaaag aagtcaagaa
     3961 aagagcgaag gaagcaagtc gtttaactcg agaaatgaaa ggcttgattt ttaccttagc
     4021 tatcgaagcg gcagttgtag tttgtaccaa tacagctatt accatccgta ttccaagttt
     4081 gatggtggaa agaggattgg gggatgccca gttatctagt tttgttctta gtatcatgca
     4141 gttgatcggg attgtggctg gggtgagttt ttctttcttg atttctatct ttaaagagaa
     4201 actgctcctc tggtctggta ttacctttgg cttggggcaa atcgtgattg ccttgtcttc
     4261 atccttgtgg gtggtagtag caggaagtgt tctggctgga tttgcctata gtgtagtctt
     4321 gacgacggtc tttcaacttg tctctgaacg aattccagct aaactcctca atcaagcaac
     4381 ttcatttgct gtattaggct gtagtttcgg agcctttacg accccatttg ttctaggtgc
     4441 aattggctta ctaactcaca atgggatgtt ggtctttagt atcttaggag gttggttgat
     4501 tgtaatctct atctttgtca tgtacctact tcagaagaga gcttaggatt gattcctaag
     4561 tttttctttt gatactcaat gaaaatcaaa gagcatagtt gattgagttt ggaatagtac
     4621 gctgtagctg ctaaaatatt tctagaaatt aatttgactt ttctaataga gttattcata
     4681 tcttatttca atctactata gaaagctagc cgcaggttgc tcaaaatagt gttttgaggt
     4741 tgcagatgga aactgacgtg gtttgaagag attttcgaag agtatgagat ttttataccc
     4801 atacaatatt tgttttaggt ccttgtttac agactaattt cttgataaaa tagaggttat
     4861 gacgagatat aaagcaacta tttcctatga tggttatgcc tttgctggct ttcagcgcca
     4921 gtctcatgcg cgtagcgttc aggaagaaat tgaaaaaacc ttgaccagat taaataaagg
     4981 gcaaaccatt actgttcacg gtgctggtag gacagatagt ggggttcatg ccctgggaca
     5041 ggtcattcat tttgacctgc cttatcagat ggatgaggag aaactccgtt ttgccttgga
     5101 tacccagtct cctgaagata ttgatgtgat ttcgattgag cttgtggcag atgattttca
     5161 ttgccgttat gccaaacata gcaagaccta tgagtttatt gtggatagag gacgtcccaa
     5221 aaatcctatg cgccgtcact atgccaccca ctttccctac ccactcgatg tggaacgaat
     5281 gcagattgca atcaaaaagc tagagggaac ccatgatttt accggtttta cagcctctgg
     5341 gactagtgta gaggataagg ttcgcaccat cacagaagct agtttaatag tcgatgagac
     5401 aggacaattt ttgaccttta ccttttcagg aaatggtttc ttgtataaac agattcgcaa
     5461 tatggtgggg actcttctca aaatcggtaa caaccgcatg ccagtagagc agattgatct
     5521 catcttggag aagaaggaca ggcaacttgc aggtcccact gcagcaccaa atggtttgta
     5581 tttaaaggag attcgttatg aagaataatc gtattttagc actttctgga aatgatattt
     5641 ttagtggtgg tggactgtca gctgatttgg ctacctatac cttgaacggc ttgcatgggt
     5701 ttgtagcagt gacttgtttg acagccttga cagaaaaagg atttgaagtc tttccaactg
     5761 atgataccat ttttcaacat gaattagata gcttgcgtga tgtggaattt gggggaatta
     5821 agattggtct tctccctact gtcagtgtgg ctgagaaggc cttggacttt atcaaacaac
     5881 gcccaggagt acctgtggtg ttggatcctg tcttggtctg caaggaaacg catgatgtag
     5941 ctgtcagtga gctctgccaa gagttgattc gctttttccc ttatgtcagt gtgattacgc
     6001 ctaatctccc agaagcagaa ttattatccg gtcaggaaat taaaaccttg gaagacatga
     6061 aaactgcagc gcagaaattg catgatttag gagctccagc agtcattatc aagggaggca
     6121 atcgtcttag tcaggacaag gcggtggatg tcttttatga tggacagacc tttactatcc
     6181 tagaaaatcc agttatccaa ggccaaaatg ctggtgcagg ttgtaccttt gcctctagca
     6241 ttgccagtca cttgattaaa ggtgataaac ttttgccagc agtagaaagc tctaaggctt
     6301 tcgtttatcg tgctattgca caagcagatc agtatggagt aagacaatat gaagcaaaca
     6361 aaaacaacta aaatcgccct tgtatcccta ttaaccgccc tttctgtggt tctaggttat
     6421 ttcttaaaaa tcccaacacc tacaggaatt ctaactcttt tagatgctgg tgtcttcttt
     6481 gcggcctttt actttggtag tcgtgaagga gcggtagtcg gaggactagc aagtttcttg
     6541 attgacctct tatcaggcta ccctcagtgg atgttcttta gcttggtcaa ccatggcttg
     6601 cagggatttt tcgcaggatt taaaggaaaa agtcagtggt taggccttat tttagcaatt
     6661 attgccatgg taggaggcta cgccttgggt tctactttga tgaatggctg ggcagcagcc
     6721 ctcccagaaa ttctaccaaa tttcatgcaa aatatggtag ggatgattgt aggatttatt
     6781 cttagtcaaa gtatcaagaa gattaagtaa tactcttcga aaatctcttc aaaccacgtc
     6841 agcttccatc tgcaacctca aaccactgtt ttgagcagcc tgaggctagc tttctagttt
     6901 gcactttgat tttcattgag tataaagagg ttgagtaaaa agtatttcaa ataagaaaaa
     6961 cgcatagtat caggtgttga atgaactgcc ccccaaaagt tagacagaaa aaatctaact
     7021 tttggggtgt tttattatga aactaactta tgatgataaa gttcagatct atgaacttag
     7081 aaaacaagga tatagcttag agaagctttc aaataaat
//
LOCUS       DAVKQB010000077         6618 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKQB010000077 DAVKQB010000000
VERSION     DAVKQB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.077"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..488
                     /locus_tag="VW528_001978"
                     /pseudo
     CDS             1..488
                     /locus_tag="VW528_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822207.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            416..868
                     /locus_tag="VW528_001979"
     CDS             416..868
                     /locus_tag="VW528_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912746.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1687..2046
                     /locus_tag="VW528_001980"
     CDS             1687..2046
                     /locus_tag="VW528_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912747.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            2056..2421
                     /locus_tag="VW528_001981"
     CDS             2056..2421
                     /locus_tag="VW528_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU9912748.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSRLISQEQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSH"
     gene            2408..4258
                     /locus_tag="VW528_001982"
     CDS             2408..4258
                     /locus_tag="VW528_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU9912749.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSYEEMVEMYNVNFTNNDKLYEYRNDRQEKHQQNIHAHHLIQSFSPEDNLTPEEINR
                     IGYETIMELTGGRFRFIVATHTDKDHIHNHILINAIDCNSDKKLIWNYALERNLRMIS
                     DRISKMAGAKIIEKRFSYRDYQKYRATSHKFELKQRLYFLMQQSKSFDDFLEKAEQLH
                     VHIDFSQKHSRFMMTDRAMTKPIRGRQLSKRDLYDEDFFRMHFTKQEIASRLEFLLNC
                     VNSLEGLLTKSKELNLTIDLKQKNVIFILEENGKQFSLSHKKISDEKLYDVNFFQDYF
                     KNKEVGVSEGIENLQAQYRAFQEERDKEKVSTEEIEEAFETFKEKRDAVHEFEVKLTE
                     HQIEKLVDEGIYIKVSFGINQSGLIFIPNYQLDIMEEENQKKYKVYIRETTSYFVYNK
                     EHSDKNQYIKGRTLIRQLTNDSRVIPYRRPTVERLQEKISEISLLIELTETDKKYQDI
                     KDNLVSEIAELDIKLTQTNEKIATLNKMAEVLINSKSEGSGSQKLARHEFSKLNMTES
                     TTLEQVNEELLKLQQEFGNVLDEYEKTIRKLGQLFKVFDECINKEIMNEI"
     gene            4514..5946
                     /locus_tag="VW528_001983"
                     /pseudo
     CDS             4514..5946
                     /locus_tag="VW528_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886556.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(6184..6318)
                     /locus_tag="VW528_001984"
                     /pseudo
     CDS             complement(6184..6318)
                     /locus_tag="VW528_001984"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         2307 a         1052 c         1298 g         1961 t
ORIGIN      
        1 agcagaggca atcataaaaa atggaataga aatagttgta gtaactgatc ataatactac
       61 caaaggtatt aaaaagttac aaatggcagt ctcaatcata atgaaaaatt atccgattta
      121 tgatatacat cctcatattt tacatggagt agaaattagt gcagcagata aattgcatat
      181 tgtatgtata tatgattatg aacaagaatc atgggttaat caatggttaa gtgaaaatat
      241 tataagtgag aaagatggaa gttatcaaca ttcactgact ataatgaagg atttcaataa
      301 tcaaaaaata gttaactata ttgctcattt caatagttat gacattttga aaaaaggttc
      361 tcacttatca ggtgcatata aacgaaaaat tttttctaaa gaaaatacac gatttttgga
      421 gtttaatatt aactcgaaag aatcttcgca acaacttgat attctctata aagaagttgg
      481 tgtattaagt ttgggacaaa aagttgtagc catgcttgat tttttattag catatagtga
      541 ctattctaaa gacttcagac cattgattat tgatcagcct gaagacaatc tagacaatcg
      601 ttatatttac aggcatttag ttcagcagtt tagagatgtg aaagctcaac gtcaaattat
      661 tttagcaaca cataatgcta caattgtaac aaattctatg acagatcaag ttgttattat
      721 ggagtcagat ggagttaacg gatggattga atcacaggga tatgttagtg aaaaatatat
      781 aaaaaatcat atcatcaatc aattagaggg aggaaaagat tccttcaagc ataaaatgtc
      841 tatatatgag acggctttat cagagtagag tcagaaaaag taggttagaa atttagccta
      901 cttttttctt tgttcgacag gcatagtgta catctgaggt ccaagtcctc tgtggatatt
      961 tgctgcagat gaaaccaata gcgactccta agcctgaata tcgtgaggta ggggggatag
     1021 gaaggaatta gcgaaatcaa ggttctacaa acagaatcgt gacttgaagc catatatagc
     1081 ggatgaggaa ctctaaaatc caaataggtg tcgtaaccta tatacgtaaa ttacgagagt
     1141 aaactaggaa agatgtacgg cttattccgt gagcgtttag gacgtagtac aacgaatcat
     1201 gggagtcagc tgaacacata gtattgaaga aatttctgta atggaaatgg agcgaagaag
     1261 tgaacaatta aatgaatacc tctctaatta aatttgtcaa ttctaattcc tggtatgaaa
     1321 agacagtgac ctaaaaatgt aaacgatggg agctgatcat aaatatagga cggtacatgc
     1381 agtggtgtta gagattagtc cctacttgat ttgtgataac ttccccaact ttcttctgct
     1441 atacttttct caacttttaa aaatccaact aagaatttta cctgggagtt tgggggcgta
     1501 gccaccaagt tatcttatgg ttagctgtca aaactggaag gttttggtta gctggcgata
     1561 tgattttttg ggtattgtga ccacaatgcc tgagctcgca aagaccgaac aaggacttta
     1621 agaaatttcc cgtatgacac acggggtcgg ggtttacccg agaaaggatg aaagtgagga
     1681 aaagagatgt ctgaacagta ccgtgacatt cgaaaagagg taaatctaac cgcagatgag
     1741 ctaaaacaga ttgaaaaaat gatggaggtt gataattatc gtcacttttc accattcgtc
     1801 agggataaaa ttttgatgac tgatgacaaa cagttagctg ccaaagaatg gttttccctt
     1861 tggcagtctc aaaagtttga acagatcagt cgagatgtgc atttggtttt gattatcgca
     1921 agagagaatc accaagtgac tcaagaacac gtgtcaatct tattgacctg cgttcaagaa
     1981 ttgattgcgg aagtcaatca agtacagtca ctcagtcgtg ggtttcgtga aaaatacatg
     2041 agataggagg ctactatggt ttatcgttat cgtaccaatc tcaaaaaagt atttctaaca
     2101 gattcagaat tacaccaatt gaatgaacgg attgctaaga gcaattgtca aaatttttca
     2161 gtctatgcca gaaaagtttt gcttaatccc aatatgtcat ttgtcacgat taacacggat
     2221 acttatgacc agttagtttt tgaattgaga cggatcggaa ataacattaa tcaaattgcg
     2281 cgtgctatca atcaaagccg tctgatttct caggaacagt tacaagaatt gagtaaagga
     2341 gtcggagagt tgatcaagga agtggacaaa gaatttcaag tggaagtgaa aagattgaag
     2401 gagtttcatg gtagtcacta agcattttgc gacacacgga aaaaaatatc gtaggcgttt
     2461 aattaagtat atcctcaatc ctgataaaac ggacaatttg aaattagtat ctgattttgg
     2521 tatgagcaat tacttagact ttcctagcta tgaagaaatg gtagaaatgt acaatgtcaa
     2581 ctttaccaat aatgacaagt tgtacgaata cagaaatgac cgacaagaaa aacaccaaca
     2641 aaatattcat gcccatcacc tcattcaatc attttctcct gaagataatc tgacacctga
     2701 agaaattaac cgcattggtt atgagaccat tatggaatta acaggaggcc gttttcgttt
     2761 catcgtagcg actcatacag acaaagatca tattcataat cacatcttaa tcaacgccat
     2821 tgactgtaat tcagataaaa agttgatatg gaattatgcc ttggaacgaa accttcgtat
     2881 gatttcagac cgcatttcta aaatggctgg ggcgaaaatt attgaaaagc gtttctctta
     2941 ccgtgactat caaaaatata gagcgactag ccataagttt gaactaaagc aacgtcttta
     3001 ttttttgatg cagcagtcaa aatcctttga tgatttttta gaaaaagcag agcaattaca
     3061 tgttcatatt gattttagtc agaagcatag tcgattcatg atgacggatc gggcaatgac
     3121 aaaaccaatt cgaggacgtc aactcagtaa acgagattta tatgatgaag atttttttag
     3181 aatgcatttt accaagcaag agattgcaag tcgtttagaa tttttgttga actgtgtcaa
     3241 ttctttagaa gggttactaa caaaatcaaa agaattgaat ctaaccattg acttaaaaca
     3301 aaaaaatgta atctttatac ttgaagaaaa tggaaagcaa ttcagtctga gtcataaaaa
     3361 aataagtgat gagaaattat atgatgtcaa tttttttcaa gattatttta aaaataagga
     3421 agtaggtgtt tcagaaggaa tagaaaatct acaggcacag taccgtgctt ttcaagaaga
     3481 acgagataag gagaaggtat ctactgaaga aattgaggaa gcctttgaga catttaagga
     3541 aaaacgagat gccgttcatg aatttgaagt aaaacttaca gaacaccaaa ttgagaagtt
     3601 agttgatgag gggatttata tcaaagtgtc ctttggtata aatcagagtg gtcttatttt
     3661 cattcctaac tatcaactgg atattatgga agaagaaaat cagaaaaaat ataaagtcta
     3721 cattcgtgag acaacctcat attttgtcta taacaaagaa cattcggata agaatcagta
     3781 tatcaaaggg agaaccttga ttaggcagct aaccaacgat agtcgagtaa taccatacag
     3841 aagaccgact gttgaaagac ttcaagaaaa gatttctgag attagcctct tgattgagtt
     3901 aactgagaca gataagaaat accaagacat taaagacaac ttggtttccg aaatagcaga
     3961 gctagatata aaactgactc aaacaaatga aaaaattgcc accttaaata agatggcgga
     4021 agtacttatc aattctaaga gtgaaggttc gggtagccaa aaactagcta ggcatgaatt
     4081 ttctaaattg aacatgacag aatcaacaac tttagaacaa gtcaatgaag aacttttgaa
     4141 gttacaacag gaatttggta atgttcttga tgagtatgag aaaacaataa gaaagcttgg
     4201 acaattattt aaggtttttg atgagtgtat caataaagaa attatgaatg aaatataaat
     4261 cagactgaag acaaactcaa atttttttgg atatcttttc tgtttatggt tgccaatatc
     4321 ggtttaactt ctaaaacaaa cattaataaa aatgcattca aagacgattt tacctatttt
     4381 acgattacgc ttcgacccca aatcattaaa gttgtctatg ctctatcatt gcatcactgg
     4441 acttgtcaag agttaagata attcggagtc cactgaaaaa gtcatcgtat gatggtatac
     4501 taaagatgag gtgatggtat gcttgataat cgatgttagt ccctattcta ctctgtgtga
     4561 tattgttgtt cccaaaactc attttttacg acagctaatg gaactctgtg attttagttt
     4621 tatttatgac gagctggaaa agaattatca acctgacttc ggatgtcgtt cctactctct
     4681 ccttatcatg atgttcaagt atctcttatt aaaagacatc tataagttgt ccgatgtgga
     4741 tgtggtggag cgctcctttt ctggtatgac cttcaaatac ttccttggtc tagctcctgt
     4801 tattgaaccg tcctcattga ctaaattccg aaaacttcgg aacaaagatg aacgattact
     4861 tgatttgctc atcgctaaat ctgttcagat tgcgatagag ctcggtctga ttaaaagtaa
     4921 cattctcatt gtggatgcga ctcataccaa agttcattac aatcacaaga aacctcaaga
     4981 agtcctccga gaacgctcaa aagccttgcg taaaacgatt tatcagtatt cggaatatat
     5041 caaggcagaa tttcctagta agcctcagga agatactcta gtagctgaat taagatatac
     5101 acaagaagta atatcagtcc ttgagaagca tgacgaattg actggaatac cagctatctc
     5161 acaaaattca attaccttaa agaagctgta gaggatgact tagagcactt ggaatcctct
     5221 gtcaaagaag aagcaagaat tggacataag tcagccgata gttcctttta cgggtacaaa
     5281 gagcattttg ccatgaccga tgaacggatt attactgcct gtgtggtgac atcgggtgaa
     5341 aaaagtgatg gtcctgtttt agaagaactc tatcacaaat ctaaggataa tggcgtgacg
     5401 attgaagcta ttgttggaga tagggcctac tctgggaagg ataatatgca atttaccaag
     5461 aaagaacgtg tgcattgagt cgctaggcca tatccaacta tttcaaaagg ctttcgtagg
     5521 aaggaagatg agtttttcta taacaaggac gcaggtctct ttgtttgtcc agaagggcat
     5581 atggcactaa agaaggaaaa gacaggaaga aggacggctc aggaaaccca ctattttgac
     5641 atcaagcact gtcaaatatg cccttcaaaa gtgggctgtt ataaagaagg ggctaagtct
     5701 aagacttatt tgatacccct taagagcgat gaacatattt tccagaagag gtttcaggaa
     5761 gcgccctatt ttaaagaaaa ggccaaacat cgctacaaga tagaagccaa aaatgcggaa
     5821 cttgaaaaca gacatggtct taatgtagct agagcaccag gtcttttaaa catggagtta
     5881 caagccgcca caaccatatt tgtggttaat atgaagagga ttataactct aataaaccaa
     5941 aaataaggaa aagcaaagag gaatctatcc aagcaggaga tagatccctc ttttttcatt
     6001 ttagttgaac tgtagagctt actttttcag tggactcgat gattagtgac tatttttttc
     6061 aaactctatt agttattggt accctaatgc cgaatggaga tatttaggat tgtaatagat
     6121 ttcaatatat ttaaagattt cgttcaatag ttgcgaaatt ggcatcattg acaagttctc
     6181 tttttatgtt ttgtaaaagg attccatggg tgcattatca tatggattgc cttttcgact
     6241 caggctagac ttgcatcttt tctgtttaag gagttcttga aatctagctc cagtatattg
     6301 agatccttga tcggtatggc cgactagttc ttatttgaga ttttctctat gataagcttg
     6361 attgaaaaca ttcgttacca acttatcttg cattcgattt cccattgcct aaccaacaat
     6421 ttttctgcta taaacatata tataatgata aataaagagt acctgctttt gtcggaatat
     6481 aagttaaatc ccccagccag atttcatttt taccttttgt ttgaaaacac tgattaatca
     6541 aattgggtcg tgttaatgag gaatgccgac ggctatagta cttgtattta tagcggattc
     6601 ctttagcata gagacaaa
//
LOCUS       DAVKQB010000078         6467 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKQB010000078 DAVKQB010000000
VERSION     DAVKQB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.078"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(73..165)
                     /locus_tag="VW528_001985"
                     /pseudo
     CDS             complement(73..165)
                     /locus_tag="VW528_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(201..484)
                     /locus_tag="VW528_001986"
                     /pseudo
     CDS             complement(201..484)
                     /locus_tag="VW528_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(474..614)
                     /locus_tag="VW528_001987"
     CDS             complement(474..614)
                     /locus_tag="VW528_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU9912750.1"
                     /translation="MVSCHSSLRSYIAEPILKQAGFTVQNLDGAYSLYKMVNPEGVEY
                     GN"
     gene            706..1470
                     /locus_tag="VW528_001988"
     CDS             706..1470
                     /locus_tag="VW528_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9912751.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIERVLD
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            2035..3414
                     /locus_tag="VW528_001989"
     CDS             2035..3414
                     /locus_tag="VW528_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9912752.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            3428..4093
                     /locus_tag="VW528_001990"
     CDS             3428..4093
                     /locus_tag="VW528_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9912753.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            4228..4302
                     /locus_tag="VW528_001991"
                     /pseudo
     CDS             4228..4302
                     /locus_tag="VW528_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            4448..4987
                     /locus_tag="VW528_001992"
     CDS             4448..4987
                     /locus_tag="VW528_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9912754.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            5054..5464
                     /locus_tag="VW528_001993"
     CDS             5054..5464
                     /locus_tag="VW528_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9912755.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIELTTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            5580..>6467
                     /gene="pavB"
                     /locus_tag="VW528_001994"
     CDS             5580..>6467
                     /gene="pavB"
                     /locus_tag="VW528_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9912756.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLV"
BASE COUNT         1755 a         1239 c         1481 g         1992 t
ORIGIN      
        1 aaaacagtgt tttgagctga cttcgtcagt tccatccaca accttaaaac agtgttttga
       61 gctgacttcg tcagttccat ccacaacctt aaaacagtgt tttgagcagc ctgcggctaa
      121 cttcctagtt tgcactttaa ttttcattga gtattacttc atcttctcat gaaattgagt
      181 ttttgcccag ctcctcttga ttattctact tgaccgggcc aagcattcat accaccttct
      241 acgttggtaa cggtgaggcc ttgggagctg agaaattggc aggtagatgc agagcggact
      301 cctccgtgac aaatgacata gtattcatgg tcaggtttga gttctttgta gccttgctct
      361 aaggtattta tggaagattt ttgggtcctg cttggaattc atgtgcttca cggacatcga
      421 taagttccag attttcattt tgatattttc tataaaagtc agccatgctg atactagttt
      481 ccatattcta ctccttctgg gttaaccatt ttgtatagtg aataagcgcc gtcaaggttt
      541 tggacggtaa atcctgcttg cttgaggata ggctctgcga tatagctgcg caaactactg
      601 tggcaactga cgatgtaggc ttgtttttct cctcagttca tcagcttcct ccttgacact
      661 cggtcagatt ttgatacaat agtacaaaat tagaggaggt gggctatgat tcagaaacat
      721 gcgattccca ttttagagtt tgatgataat cctcaggcgg ttatcatgcc caatcacgag
      781 gggttggatt tgcagttgcc aaagaagtgt gtctatgctt ttttaggtga ggagattgac
      841 cgctatgcga gggaagtagg ggcgaactgt gttggcgaat tcgtttctgc taccaagacc
      901 tatccagtct atgtcatcaa ctacaagggt gaggaggtct gtctggctca ggctcctgtt
      961 ggctccgctc cagcagccca gtttatggat tggttgattg gctatggtgt ggagcagatt
     1021 atctctactg ggacctgtgg tgtcctagct gatatagagg aaaatgcctt tctagtccct
     1081 gttcgcgctc tgcgagatga aggagccagt taccactatg tggcaccttc tcgttatatg
     1141 gaaatgcagc cagaggctat tgctgccatt gagcgagttt tggatgatag agggattcct
     1201 tatgaagaag tcatgacctg gtccacagat ggtttttacc gagaaacggc tgaaaaggtg
     1261 gcttatcgta aggaagaagg ctgtgctgtt gtggagatgg agtgttctgc tcttgcggca
     1321 gtagctcaat tgcgtggagt tctctggggt gaattgttgt tcacagcaga ttctctagcg
     1381 gacttggacc agtacgacag tcgtgactgg ggctcggaag cttttaataa ggcactagaa
     1441 ctgagtttag caagtgttca ccacctttag ttgtactggc aaaggatttg ttttatcata
     1501 aaatgtctag ctcatacttt tcaaaaatat gtttaaacga ggtcaccttc ctcttgtcct
     1561 aggcatgttg aggttgggaa aaatctttaa aatcagaaaa acgtatcata tcaggtgatg
     1621 aaaattttga tactatgcgt tttatgttgg taagatttag agtgagatga aatgatactc
     1681 ttcgaaaatc tcttcaaacc aggtcagctt caccttgccg tatagatgtt actgacttcg
     1741 tcagttctat ccacaacctc aaaacagtgt tttgagcaac ctgtgactag ctttctaagc
     1801 gatgccttgg ttctcattgc ctataatcaa aaagagaaat tttctcctga aaagcatata
     1861 gagtagctgg cgttaaaagc tcctgtcttg cttttttgac ctatagtcac atctatcaag
     1921 tattgttctt gcctaagcta tcaataaaaa ggtggcattt tttaggcttg gtgttagtag
     1981 attttgcctt atcctatcta agtcatttcg aactttttat ggtacaatgg aaacatgtta
     2041 ttcaaattat ctaaggaaaa aatagagcta ggcttatctc gtttatcgcc agcccgtcgt
     2101 atttttttga gttttgcctt ggtcatttta ctaggctctc ttcttttgag cttgcccttt
     2161 gtccaagttg aaagctcacg agcgacttat tttgatcatc ttttcactgc tgtctctgca
     2221 gtctgtgtga caggtctctc aacccttcca gtagctcaca cctataatat ctggggccaa
     2281 ataatctgtt tgctcttgat tcagatcggt ggtctagggc tcatgacctt tattggggtt
     2341 ttctatatcc agagcaagca aaagcttagt cttcgtagcc gtgcaactat tcaggatagt
     2401 tttagttatg gagaaactcg atctttgaga aagtttgtct attctatttt tctcacgacc
     2461 tttttggttg agagcttggg agctattttg cttagttttc gccttattcc tcaacttggc
     2521 tggggacgtg gtctttttag ttccattttt ctagcgatct cagccttctg taatgccggt
     2581 tttgataatt tagggagcac cagtttattt gcttttcaga ccgatttact ggtcaatctg
     2641 gtgattgcag gcttgattat tacaggcggc cttggtttta tggtctggtt tgatttggct
     2701 ggtcatgtag gaagaaagaa aaaaggacgt ctgcactttc atacgaagct tgtactatta
     2761 ttgactatag gtttgttgtt atttggaaca gcaactactc tctttcttga gtggaacaat
     2821 gctggaacga ttggcaatct ccctgttgcc gataaggttt tagttagctt ttttcaaaca
     2881 gtgacgatgc gaacagctgg cttttctacg atagattata ctcaggctca tcctgtgact
     2941 cttttgattt atatcttaca gatgtttcta ggtggggcac ctggaggaac agctggggga
     3001 ctcaagatta cgacattttt tgtcctcttg gtctttgcac gaagtgagct tctaggcttg
     3061 cctcatgcca atgttgcgag acgaacgatc gcgccgcgaa cggttcaaaa atcctttagt
     3121 gtctttatta tctttttgat gagcttcttg ataggattga ttctgctagg gataacagcc
     3181 aaaggcaatc ctccctttat ccacctcgta tttgaaacca tttcagctct tagtacagtt
     3241 ggtgtaacgg caaatctgac tcctgacctt gggaaattgg ctctcagtgt tatcatgcca
     3301 cttatgttta tgggacgaat tggtcccttg accttgtttg ttagcttggc agattaccat
     3361 ccagaaaaga aagatatgat tcactatatg aaagcagata ttagtattgg ttaagaaagg
     3421 aaagagcatg tcagatcgta cgattggaat tttgggcttg ggaatttttg ggagcagtgt
     3481 cctagctgcc ctagccaagc aggatatgaa tattatcgct attgatgacc acgcagagcg
     3541 catcaatcag tttgagccag ttttggcgcg tggagtgatt ggtgacatca cagatgaaga
     3601 attattgaga tcagcaggga ttgatacctg cgataccgtt gtagtcgcga caggtgaaaa
     3661 tctggagtcg agtgtgcttg cggttatgca ctgtaagagt ttgggggtac cgactgttat
     3721 tgctaaggtc aaaagtcaga ccgctaagaa agtgctagaa aagattggag ctgactcggt
     3781 tatctcgcca gagtatgaaa tggggcagtc tctagcacag accattcttt tccataatag
     3841 tgttgatgtc tttcagttgg ataaaaatgt gtctatcgtg gagatgaaaa ttcctcagtc
     3901 ttgggcaggt caaagtctga gtaaattaga cctccgtggc aaatacaatc tgaatatttt
     3961 gggtttccga gagcaggaaa attccccatt ggatgttgaa tttggaccag atgacctctt
     4021 gaaagcagat acctatattt tggcagtcat caacaaccag tatttggata ccctagtagc
     4081 attgaattcg taaagaggga tgacccctct tttttgatgc ctaagatggc aaatagagac
     4141 agaagcccct tgtcttctag taaaagttct tcaaaggctg gactttatgg taaaatagaa
     4201 agaagtgaca agagagagta atactcaatg aaaatcaaag atcaaactag gaaactagct
     4261 acgggctgct caaaacactg ttttgaggtt gcagatagaa ctgacgaagt cagctcaaaa
     4321 cactgttttg aggttgcaga tagaactgac gaagtcagta acatctatac ggcaaggcga
     4381 cgttgacgcg gtttgaagag attttcgaag agtataagaa aaaatcagtc ccctaaagga
     4441 gtagattatg aagttattgt ctatcgcaat ttctagctat aatgcagcag cctatcttca
     4501 ttactgtgtg gagtcgctag tgattggtgg tgagcaagtt gggattttga ttatcaatga
     4561 cgggtctcag gatcagactc aggaaatcgc tgagtgttta gctagcaagt atcctaatat
     4621 cgttagagcc atctatcagg aaaataaatg ccatggcggt gcggtcaatc gtggcttggc
     4681 agaggcttct gggcgctatt ttaaagtagt tgacagtgat gactgggtgg atcctcgtgc
     4741 ctacttgaaa attcttgaaa ccttgcagga acttgagagc aaaggtcaag aggtggatgt
     4801 ctttgtgacc aattttgtct atgaaaagga agggcagtct cgtaagaaga gtatgagtta
     4861 cgattcagtc ttgcctgttc ggcagatttt tggctgggac caggtcggaa atttctccaa
     4921 aggccagtat accatgatgc actcgctgat ttatcggaca gatttgttgc gtgctagcca
     4981 gttctaactg cctgaacata ctttttatgt cgataatctc tttgtcttta cgccccttca
     5041 gcaggtcaag accatgtact atctgcctgt cgatttctat cgttatttga ttgggcgtga
     5101 ggaccagtct gtcaatgagc aagtgatgat taagtgcatt gaccagcaac tcaaggtcaa
     5161 tcgactcttg atagaccaac ttgatttgtc ccaagtgagt catcccaaaa tgcgagaata
     5221 tctgctgaat catattgaac tcacgacggt gatttccagt accctgctca accgatctgg
     5281 aacagcggag catctggcaa aaaaacgcca attgtggacc tatattcagc agaaaaatcc
     5341 agaagtcttt caggctattc gtaagaccat gttgagccgt ttgaccaaac attctgtctt
     5401 gccagatcgc aaactgtcca atgtcgtcta tcaaatcacc aaatctgttt atggatttaa
     5461 ttaatataag tgttttataa gagggattta agaaaaattt taactttttc ttagtccttt
     5521 ttaatttcag gagattatac tagagtcatc aaataaagaa agactctaag gagaatccta
     5581 tgaaattcaa tccaaatcaa agatatactc gttggtctat tcgccgtctc agtgtcggtg
     5641 ttgcctcagt tgttgtggct agtggcttct ttgtcctagt tggtcagcca agttctgtac
     5701 gtgccgatgt ggtcaatccg accccaggtc aagtcttacc tgaagagaca tcgggaacga
     5761 aagagggtga cttatcagaa aaaccaggag acaccgttct cactcaagcg aaacctgagg
     5821 gcgttactgg aaatacgaat tcacttccga cacctacaga aagaactgaa gtgagcgagg
     5881 aaacaaactc ttctagtctg gatacacttt ttgaaaaaga tgaagaagct caaaaaaatc
     5941 cagagctaac agatgtctta aaagaaactg tagatacagc tgatgtggat gggacacaag
     6001 caagtccagc agaaactact cctgaacaag taaaaggtgg agtgaaagaa aatacaaaag
     6061 acagcatcga tgttcctgct gcttatcttg aaaaagctga agggaaaggt cctttcactg
     6121 ccggtgtaaa ccaagtaatt ccttatgaac tattcgctgg tgatggtatg ttaactcgtc
     6181 tattactaaa agcttcggat aatgctcctt ggtctgacaa tggtactgct aaaaatcctg
     6241 ctttacctcc tcttgaagga ttaacaaaag ggaaatactt ctatgaagta gacttaaatg
     6301 gcaatactgt tggtaaacaa ggtcaagctt taattgatca acttcgcgct aatggtactc
     6361 aaacttataa agctactgtt aaagtttacg gaaataaaga cggtaaagct gacttgacta
     6421 atctagttgc tactaaaaat gtagacatca acatcaatgg attagtt
//
LOCUS       DAVKQB010000079         6140 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKQB010000079 DAVKQB010000000
VERSION     DAVKQB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.079"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(116..391)
                     /locus_tag="VW528_001995"
     CDS             complement(116..391)
                     /locus_tag="VW528_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9912757.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(498..1337)
                     /locus_tag="VW528_001996"
     CDS             complement(498..1337)
                     /locus_tag="VW528_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9912758.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1479..2327
                     /locus_tag="VW528_001997"
     CDS             1479..2327
                     /locus_tag="VW528_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9912759.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2420..3337)
                     /locus_tag="VW528_001998"
     CDS             complement(2420..3337)
                     /locus_tag="VW528_001998"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9912760.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3683..3891
                     /locus_tag="VW528_001999"
                     /pseudo
     CDS             3683..3891
                     /locus_tag="VW528_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4201..4494)
                     /gene="rpmA"
                     /locus_tag="VW528_002000"
     CDS             complement(4201..4494)
                     /gene="rpmA"
                     /locus_tag="VW528_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9912761.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4511..4855)
                     /locus_tag="VW528_002001"
     CDS             complement(4511..4855)
                     /locus_tag="VW528_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9912762.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4871..5185)
                     /gene="rplU"
                     /locus_tag="VW528_002002"
     CDS             complement(4871..5185)
                     /gene="rplU"
                     /locus_tag="VW528_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9912763.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5221..5308)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5373..6071)
                     /locus_tag="VW528_002003"
     CDS             complement(5373..6071)
                     /locus_tag="VW528_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9912764.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKA"
BASE COUNT         1875 a         1255 c         1025 g         1985 t
ORIGIN      
        1 atacaatatt ttcggaacat tcaacttttt aactctattt attactagat ttcataatta
       61 aaaagcctac tgaccaagct agaaagcttg atacaatagg ctttttaaag actgattatt
      121 taacagcgtc tttaagagct ttaccagctt tgaatgctgg tactttagaa gctgcaattg
      181 tcatttcttt accagtttgt gggttgcgac ctttacgttc tgcgcgctca cgaacttcaa
      241 agttaccaaa accgatcaac tgaacttttt caccagctgc aagatagtca gctactgctg
      301 caaatacagc ttcaactgct gctgctgagt ctttcttagt taattctgta gcttctgcta
      361 ctttagcgat caaatcttgt ttgtttgcca tgttaatgat tcctccaaat aatttctaat
      421 taacaaatat aatcatatcc taaaatccct tttaggtcaa gttaaaaacg ctatttcttc
      481 ccattttatt tattttttta ggagtggtaa cgtatcaaaa tagcccaagc gttctcaccc
      541 gtgtgagttt gaataatgga acccgtttcc aaaacagaaa ttggcttttc aacataagct
      601 tgtaagcttt ctttcatctc ttttgcccaa tcatcactac cagaatatga aattccaatc
      661 tctgctacag cacgttcaga aagcgatgtt atcaactcat ctaaccattt tttaaatgtt
      721 ttagttccac gacctttaac cattggctgc aattcatggt ctttcatttg catgacaaca
      781 cggatattga gaagagagct caacaagcca gttacacggc taattcgtcc acctttgaca
      841 agattttcca aagttgaaac accaatataa agctctgtat ggtttttaac ctcttctaca
      901 tgagataaaa ttgcctccat atctttacct tcttgagcta acttcgcagc ctcaacaact
      961 tggaatttca gggcttggtc agtgaaggaa ctatcaacaa cagtcacatc tgcagtagat
     1021 aggctagcac cttggcgtgc tgcttctacc gtacccgaaa gagcatggga catatgaata
     1081 gcaagaatct ggccaccatc tttgcatagg tcttcaaaaa tctcagcaaa gacacctaca
     1141 ggtggctgac ttgttttcgg aagattctta ctttcttgca tcaactgaag aaatttacct
     1201 tcttctttca aatccgcatc agaataaaca acattatcaa tcattacaga taatggaaca
     1261 attgtaatat ctaattgctt tactagttca ggttcaatag taacagatga atcggttaca
     1321 atcttaattt ttgtcatagt atcaatcttt ctattttagg attcagattg gtttccttac
     1381 ttctaattat atcaaaaaaa agattaaaaa tcctaatgga gtcaatcaaa ttttccgtaa
     1441 aatttgatat aatcaactta taagaaaaga ggtgtcctat gattaaaaaa atttacccca
     1501 tttttaccat tttactaggt gctgctattt atgcttttgg actgacttat tttgtagttc
     1561 cccatcatct ctttgaagga ggggcgacag gcattaccct catcaccttt tatcttttta
     1621 aaatccctgt ttccctcatg aacctgctga ttaatattcc ccttttcatc ctagcttgga
     1681 agatctttgg agccaaatcc ctctattcta gtttactagg aaccttagct ttgtccgcct
     1741 ggttagcttt ttttgagcat attccccttc atattgatct tcaaggtgat ttactaatca
     1801 cagcccttat agcgggaatc ctattgggaa ttggccttgg aattattttt aatgctggag
     1861 gtacaactgg cggaactgat attctagctc gtattctcaa caaatacact catatatcca
     1921 taggaaaact gctctttatc ttagattttt gtattctcat gttgattctc ctaatcttca
     1981 aggatttgag attggtttcc tacacgcttt tgtttgattt tattgtttct cgtgttattg
     2041 atttgattgg tgaaggagga tatgccggca aaggctttat gattatcaca aaacgtcctg
     2101 accaacttgc taaggcgatt aatgatgacc tcggaagagg tgttactttt atttctggtc
     2161 aaggctacta tagtaaagaa aatttgaaaa tcatctactg tattgtcgga agaaatgaaa
     2221 ttgtgaaaac gaaggaaatg attcatcgaa tcgatcctca agcctttata actattacag
     2281 aagcccatga aatcctagga gaaggcttca cctttgaaaa agaataaaaa gaggtaatgt
     2341 cgtgacctca aaagttagac taaatcatct atcttttggg ttacagacaa cctctttttt
     2401 gttttattta ctcaagctct taagaccaat tccgagttac ttcttcatca gcctttaact
     2461 gatccactaa ttggtcaact gagtcaaatt tggtcatatc acgaatgcga tcaagccaat
     2521 aaaccatgac ggtttcccca taaatatctt gattaaaatc aaaaatattg acttcaaaac
     2581 gtgcttcttc tccatcaaag gtcacatttt tcccgacact agccatagca cgatacttct
     2641 gtctttgaat ctcaacatca acgacataaa cgccatctgc tggcatataa gtacggtcta
     2701 aaagcactaa attcgctgtc ggataaccaa ttgtacgacc acgagcatta ccatgaacta
     2761 ccatacctct tgatggaagc ggtgccccca aaagttttcc tgcttctttc acatttccat
     2821 ctaaaatagc ttgacggata cgagttgaac taatctttcc tttctcatct tctacaggtg
     2881 gaacaatgat aacttctcca tcaaagtaat cctttaaatc ttctgctgtt tttttgtcag
     2941 aaccaaatgt ataatcaaaa cctgcaacaa taattttggc attcatagcc ttgatataag
     3001 ttgcaaagaa ttcttgtgca gtaagactag cgaattgact actaaaatca aggagatata
     3061 attcttctac accttcgcgc tttaattttc tttcacgttc agcagggttc aaaatatgca
     3121 aaaacaaatc tggatgataa ggctctaaag cgatctttgg agattcatta aaggtcataa
     3181 cgacgatagg taataaatcc tttctcgcag ccttgttggc aacacgaaat aattcttgat
     3241 gccccttatg tatgccatca aaatagccga gaacaacgac tgaatcagat ggtgtgccaa
     3301 tatctttttg gttttttata ggaatagtaa taatcataaa ataattatat catagcgata
     3361 gctatttctg gaacagaaaa tctgaaatgt tgtttttttc acatgaagtg tacctgtttt
     3421 caaaaagcac tttattctat cgttgcttaa ctatgaactt tgcaatattc ttctcaaaaa
     3481 cttgtaggac atcttcaaaa ttttgcaagg agtgattaga cttgttcggt aaccataaag
     3541 tgtcatacta tgttatgtat gaaaaagcaa tgcaactaac tcctgagaac tttaaattac
     3601 taattggtgc cgaaaaggta gaatttagaa tcgaggtaca cctatggctg taaaatttac
     3661 aaaatgagac aacttgggca agatgtttga agaatttcct aaactcccat ctagtgtgct
     3721 tcagactagg ctatttttct ctccatctgt tagcttggat tctcagaccg tttcagctaa
     3781 agaatatctt ttcccttatc agaaggaacg gctcaagcca ttcagacaag tgaagggacg
     3841 acaagccaat atttgaaacc agatagcagt tcttatagtc aattgaaata aaatctgaag
     3901 aaatcgagta ggaaactcat atcaattttt aacagtgttc tattccagat tcatactcaa
     3961 tgaaaattaa agtgcaaact aggaagttag ccgcaggctg tacttgagta cggcaaggtg
     4021 aaattgacgt ggtttgaatt tgattttcga agagtatcaa tccactatac atattaaaaa
     4081 gtatgggtat caaaataaaa aacaggctct ccgaaaactc ggaaagcctt attttaagct
     4141 gtttctagct tcctcacctt tacatttcaa ggttcgggat aaaaggttca atggaccttt
     4201 ttatttagcg attgggtaaa cagaaacttg ttttttatcg cgtcctttac gttcaaagcg
     4261 tactacgcct tcaactttag cgaacaaagt atcgtctcca ccacgaccaa cgtttacacc
     4321 tggatagatg tgtgtaccac gttgacggta aaggattgat ccacctgtta cagtttgtcc
     4381 gtcagctgct ttagctccaa gacgttttgc ttgtgaatca cgtccgtttg atgtagaacc
     4441 tccacctttt ttgtgggcga aaagttgcaa gttgttaaga gtcattttta acataatgtt
     4501 ttcctccgtg ttagttttct gtgataactc tggtttggac gaactcagaa tagttctccg
     4561 ataagtttgc catacctaag aaaaatgatt caaagaataa ctgggtcatt tctctctggt
     4621 gtgaaggaag atcttttggt atttcaacca tcagatagcc accttcatct tcgtttaatt
     4681 ctaggattgg ttcatagcct gcaaatttct caatagaatt gataaagtta atggcaagcg
     4741 tagaaaccga tgcacacacg acatctaagc cgtattcgcc actctcggcg tgtccagtaa
     4801 tttccgcact cctcagctcg ccatcttcgg ctctctcaaa gactgcctgt atcatgtgtt
     4861 ctccttaaaa ttaagcgttg attgcgttga tgacaacttt tgtatatggt tgacggtgac
     4921 cttgtttacg gtggctacct tttttaggtt tgtacttgta agtaaccact ttcttttgtt
     4981 ttccttgttt ttcaacagtt ccaactacag tagctccagc aacaagtgga gttccgacaa
     5041 cagtgttttc accaccaaca agaacaactt cgttaaaagt aacttcttga ccagcttcaa
     5101 cgttcaattt ttcaacgtaa actgcttgac caacttcaac tttaacttgt tttccgccag
     5161 ttttgataat tgcgtatgtg ctcattatgc acctcctatg atttttatgg gtttccccga
     5221 ttttttgtga agactcgcct agcatcgtgg gacgaaccac ttaaacttga ataatcaagc
     5281 aacgatgttc gtgcggttgc acaggatcgt gcatagtcaa ctcttcaagt atagcatatc
     5341 tcctattttc ttacaagtaa taacacctaa aatcaagctt tttcttttac ttttttctgc
     5401 caagaggcaa aaagcatgct gaggtaaaaa acgctcatca taataggaac accaagaatg
     5461 gtcttttcat gatagaaaat cgtcaaatag gctgaaaaga caacgccaag gacaaaacta
     5521 ctaagcaggc taacaaatat gaatccttca cgcaaaaaag gagtgtgctt ggttcggaaa
     5581 taatctccaa aagccagcat ggtccgtttg atattccctg tcataaaagc gttattatag
     5641 gcaatacccg acacttctcc aaaagcagtt gtcaccagtc ccatacagaa ggccaagggc
     5701 ggcactagat agatattatc cacagtttgc ggcacaaaag caataatgat tgataagatt
     5761 gccaagggaa tcaaggacag aataggtttt ttcacaattc tcaatttttc cttataaatc
     5821 gttaataaaa agactcccat cataaacgct agcaaggtga gaaccttgtc cctaacatcc
     5881 gaaacattat ttttaattaa ttctactgaa agaaagacaa catttccagt ttgtccagct
     5941 acaagggtat tcccgcgaac aataaaagtg taagcatcca catatccagc acaaaacgtc
     6001 aaaaaaagtg ctaacctttt agactgacgt gatatttttc ttataggtaa taacctcatt
     6061 ttacctccca ttgtattttc tcttagaaat attgtaccat tttctttcta aaaaatcgta
     6121 ggctaccatt tagattttac
//
LOCUS       DAVKQB010000080         5939 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKQB010000080 DAVKQB010000000
VERSION     DAVKQB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.080"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            411..632
                     /locus_tag="VW528_002004"
                     /pseudo
     CDS             411..632
                     /locus_tag="VW528_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(919..1401)
                     /locus_tag="VW528_002005"
     CDS             complement(919..1401)
                     /locus_tag="VW528_002005"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9912765.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(1496..2980)
                     /locus_tag="VW528_002006"
     CDS             complement(1496..2980)
                     /locus_tag="VW528_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9912766.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQLAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3133..4740
                     /locus_tag="VW528_002007"
     CDS             3133..4740
                     /locus_tag="VW528_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9912767.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDELIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            4905..5072
                     /locus_tag="VW528_002008"
     CDS             4905..5072
                     /locus_tag="VW528_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU9912768.1"
                     /translation="MKKRILSAGLTFTSALLLAACDQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(5065..5586)
                     /locus_tag="VW528_002009"
                     /pseudo
     CDS             complement(5065..5586)
                     /locus_tag="VW528_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1752 a         1117 c         1322 g         1748 t
ORIGIN      
        1 aactggttcc ttttgaggta cgacgggcat gtcgtatagt aggttgaaac tagaatagta
       61 cacatctact tctaaaacat ttttagaaat cgatttgact gtcctgatcg atttgtcctg
      121 ttcttatttc attttactat atatctgagg tgtaagtcct cggtgggcac ctgttaccgg
      181 tgaacccaat agcgattccc aagcctgact atcgtgaggt agcggattaa aacggtctgg
      241 ggatagaccg ttttaagtct gacgctggaa ataagaatcg tcagaggaag ggatagcgaa
      301 ctcgtggctc tacgaacagg aacgtgatag taaggcgtat atagcggata agggggcctc
      361 aaactctaaa gtccaaaaag gtagtcgtaa cctatatgcg taaatcacga gagcaactga
      421 atttgtccat tcgaggatgg ataaactatt tttcattggg aaatatgaaa agtatagtca
      481 ccagcataga tgagcgtttg catactcgcc tacgagtgat tatctggaag caatggaaga
      541 agaaatcgag acgattatgg ggattgctta agttaggggt tcctaaatgg atagcagata
      601 aggtatctgg ctggggcgac cactatcaat tatagtaaaa tgaaataaga atagaacaaa
      661 tcgatcagga tagtcaaatc gatttctaac aatgttttag aagcagaggt gaactattct
      721 agtttcaatc cactatagta gctcagaagt cggtacttaa acgtgctata tcaaaaccag
      781 tccttgaaaa acgtggattg gtttcgtgtt tggattatta ccttgaacga catgcgttaa
      841 aaagttagtt gaaccgccgt atgccgaacg gtacgtacgg tggtgtgaga ggggctagag
      901 attatcccct actcgatttt aaatcacatg acgttcaaag gcatcatctg aaatcccttg
      961 ttccaagatg agttttgccc attctttagc agagaagagg ctgtggtcct tgtagtttcc
     1021 gcaagattcg atggttgtcc ctgggacatc ttcccaagta gtagtttcag cgatttcctt
     1081 gagcgaatcc ttgataacag ctgcgatttt agcactggtg tgacgtcccc acataatcat
     1141 gtggaagcct gtgcggcaac caaatggtga acagtcaatc atgccgtcaa tgcgggtacg
     1201 gatgagtttg gctaagaggt gctcgatagt gtgaaggccg gcagtaggga tagagtcttc
     1261 gtttggttgc accaagcgaa tatcataatt ggagatgatg tctccttttg gtcctgtttc
     1321 ttccccaatc aagcgaacat agggtgcttt gacaatggtg tggtcaagtt caaaactttc
     1381 gacaataact tcttttgaca tggtaaatcc tttcagtttt cttctctcat tatatcataa
     1441 aggttgctcc tgagacagag agaaaacctc tccgaggctg gagaggttga aatctttact
     1501 tacgatataa gcggtcgtat tggtagtatg ggtcaaaggt tacgttgata cccagtttac
     1561 gaaggacatt cttgtcttca tcagtcaaga tgatggttga gtgggcttcg cttcctttga
     1621 ggttgccgag ttcttccata gcgcgggcag catcaggatt ttctgtagct gtgatagcaa
     1681 gtgcaatcag gatttcattt gaatgaaggc gtggattgcg gctaccgaga tgatcgattt
     1741 taagaccttg gattggctta acaacttcag gctcgattag ttttacttct ttagcgatgt
     1801 cagctgattt tttgatggcg ttgatcaagg cagcggctgt aggcccaaag agttctgagt
     1861 tcttaccagt gacgatttcc ccatttggca attcaaaggc tagggctggt ccaccagttt
     1921 cttctgcttt ttggcgcgca acgacagcaa ccttacggtc tgcaggtgtg ataccgaggt
     1981 cgttcatgag caactcaatt ttcttgacgg cagcttcgcc aactttttca gctttgaagt
     2041 caagaactgt ttgatagtaa cggcggatga tttcttgttt agaagcttcg acagcagcct
     2101 cgtcatctgt aatagcgaaa ccaaccatgt tgacacccat atctgtcggt gaagcgtatg
     2161 gtgattttcc gagaatacgt tccaacatgc gcttgagcac tgggaagatt tcgatatcac
     2221 ggttgtagtt gacagtggtt tctccatagg tttgaagatg gaaggggtca atcatgttga
     2281 catcatcaag gtcagctgtg gcagcttcat aagccaagtt aactggatga tgaaggggaa
     2341 gattccaaac agggaaggtt tcaaatttag cgtagccaga cttgatgcca ttgatttggt
     2401 cgtggtacat attggacata cacgttgcca attttccaga accgggtcca ggagcggtta
     2461 cgacaatcaa gttgcgactg gttttgatgt agtcgttttt gcccatgcct tctggggaaa
     2521 tgatgtgatc catatccgtc ggatatcctt tgattggata atgaagataa gaatcaattc
     2581 cgtttttctc aagttggttg cggaaggcat ctgcagcgag ttggccagcg tattgtgtaa
     2641 tgacaacgga accaacaaaa atccctaatt cattgaattt gtcaatcaaa cgaagaactt
     2701 cttggtcata agaaatgcct aagtcgccac gtgctttgga atgttcaatg ttgctagcat
     2761 taatggcaat cacaacctca acctgctctt tcaattcttg caagagcttg attttgttgt
     2821 caggttcata accaggaagg acacgagcag cgtggaaatc ttctaacatt ttaccgccaa
     2881 actctaagta gagcttgccg tcaaattggt taatgcgctc taagatgtgg tcgcgttgta
     2941 aattcaaata ctgttcagaa ctaaaagctt gttttttcat ttttttacct ctggattcta
     3001 ttataataaa aaattggaag ttaggaaact acggagctaa aaaagaaatt aaaaagatta
     3061 agcaaacgct tgcacaaaat tttaaaaagt gctatcatat actatagatt attaaaataa
     3121 tgaggtaaac agatgcaaga aaaatggtgg cataatgccg tagtctatca agtctatcca
     3181 aagagtttta tggatagtaa tggagatgga gttggtgatt tgccaggtat taccagtaag
     3241 ttggactatc tagctaagct aggaatcaca gcgatttggc tttctcccgt ttatgacagc
     3301 cctatggatg ataatggcta tgatattgct gattatcaag cgattgcggc tatttttgga
     3361 accatggagg acatggatga actgattgca gaagctaaga agcgtgatat ccgtatcatc
     3421 atggacttgg tggtcaatca tacctcggat gagcatgcct ggtttgtaga ggcctgtgaa
     3481 aatcctaata gccctgagcg agactactat atctggcggg atgagcctaa cgatttagat
     3541 tctatcttta gtgggtctgc ttgggaatac gatgaaaagt caggtcaata ctatctccac
     3601 tttttcagca agaaacagcc ggatctcaac tgggaaaatg aaaaacttcg ccagaaaatt
     3661 tatgagatga tgaacttctg gattgataag ggtattggtg gtttccgtat ggatgttatt
     3721 gacatgattg gcaaaattcc tgacgagaag gtagtcaata atggtcctat gctccatccc
     3781 tatctcaagg aaatgaatca ggcgaccttt ggagataagg atctcttgac agtaggggag
     3841 acttggggag caacgccaga gattgccaaa ctctactctg atccaaaggg gcaagaattg
     3901 tctatggtct tccagtttga acatatcggt cttcagtatc aggaaggtca acctaaatgg
     3961 cactatcaaa aagagctgaa tatcgctaag ttaaaagaaa tcttcaacaa atggcagaca
     4021 gagttaggag ttgaggacgg ctggaattcc ctcttctgga acaaccatga cctccctcgt
     4081 attgtctcaa tctggggaaa tgaccaagaa taccgcgaaa aatctgccaa agcctttgca
     4141 atcttacttc atctcatgag aggaactcct tatatctacc aaggtgagga gattgggatg
     4201 accaactatc cgtttgaaac actggatcaa gtagaagata ttgaatctct caactatgcg
     4261 cgtgaggctc ttgaaaaagg tgttccgatt gaagaaatca tggacagtat ccgtgttatt
     4321 ggacgtgaca atgcccgtac ccctatgcaa tgggacgaga gcaaaaacgc tggtttctca
     4381 acaggtcaac cttggttggc agttaatcca aattacgaga tgatcaacgt ccaagaagcg
     4441 ctggcaaatc cagattctat tttctatacc tatcagaaac tggtccaaat tcgcaaggag
     4501 aatagctggc taattcgagc tgactttgaa ttgcttgata cggctgataa ggtctttgct
     4561 tatatacgta aggatggcga ccgtcgcttc ctagttgtgg ctaacttgtc caatgaagag
     4621 caagacttga cagtagaagg aaaagtcaaa tctgtcttga ttgaaaacac cctagctcaa
     4681 gaagtctttg aaaaacaaat cttagttcca tgggatgctt tctgtgtgga attactataa
     4741 atattttttg cagaaaaatt taaaattgaa atcgtataaa aacaagggag gactgtataa
     4801 aaggcagaaa tcctttgttt tttataacca aggtttataa accttcattc tcgaaattca
     4861 attaacttta caaattccca ctattaagga gaaagaagac gaacataaag aagcgtatcc
     4921 ttagtgcagg cctgactttt acatctgctt tgcttttagc tgcttgcgac caatcaggtt
     4981 cagatacaaa aacttactca tcaaccttta gtggaaatcc aactacattt aactatctat
     5041 tagactatta cgctgataat atagtcaatt gaaacaagaa caagacaaaa gagcctcata
     5101 aaaggtattg caacttggta ataccttttt gaggtgcttt ttgatatgag cccatgtttt
     5161 ctcaatagga ttgtactcag gtgagtaggg aggaagaggt aaaagtttat gcccaaactc
     5221 ttcgcataaa agttctagct tccccattct atggaatctt gcattatcca taataataac
     5281 cgatggtgtg gttaatgttg gtaagaaaaa cttctgaaac catacttcaa aaaagtcgct
     5341 cgtcatcatc tcttcgtaag tcattggagc gattaattca ccatttgtta gacctgcaac
     5401 caaagaaatc ctctgatatc ttcttccaga tactttgcct cttcttaact gaccttttaa
     5461 tgagcgacca tattctcgat aaaaataagt atcgaatcct gtttcatcaa tctaaacagg
     5521 tgctaggtgc tttaaactat taaaattctt aagaaataag gctacttttt ctgggtcttg
     5581 ttcataatta gattgaaact agagtagtag acctctgctt ctaaaacatt gttagaaatc
     5641 gatttgactg tcctgatcga tttgtcatgt tcttatttca ttttactata tttttgtttc
     5701 gcgggaagtc tactaagata cttaaagatg cagatagtaa aaaaaatgtg tcaatcgaaa
     5761 gtgattatgt ccccaaaaaa tggagagttt gagggcaatt attttgattt catttgacac
     5821 gaggaaccct tgaagctgtt ttgttagcta aaccaaggct agtcttagcc ttggctcgcc
     5881 agcctaacag ctccaagcct cttatcaaat gaaatacttg tcttacactc ctcactttc
//
LOCUS       DAVKQB010000081         5905 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKQB010000081 DAVKQB010000000
VERSION     DAVKQB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.081"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            67..309
                     /locus_tag="VW528_002010"
     CDS             67..309
                     /locus_tag="VW528_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9912769.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            311..565
                     /locus_tag="VW528_002011"
     CDS             311..565
                     /locus_tag="VW528_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9912770.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHWRDIY"
     gene            612..1264
                     /locus_tag="VW528_002012"
                     /pseudo
     CDS             612..1264
                     /locus_tag="VW528_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
     gene            complement(1524..2510)
                     /locus_tag="VW528_002013"
     CDS             complement(1524..2510)
                     /locus_tag="VW528_002013"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9912771.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            2704..5172
                     /gene="gyrA"
                     /locus_tag="VW528_002014"
     CDS             2704..5172
                     /gene="gyrA"
                     /locus_tag="VW528_002014"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9912772.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            5172..>5905
                     /gene="srtA"
                     /locus_tag="VW528_002015"
     CDS             5172..>5905
                     /gene="srtA"
                     /locus_tag="VW528_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9912773.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYK"
BASE COUNT         1855 a          974 c         1291 g         1785 t
ORIGIN      
        1 tttatattga ctgaacacct aaaaagtaat acaatggtgt taccattaaa aaagggagca
       61 caaaagatga ctactattac attaaaagtt tctgaagctg ataaaacatt tatgaaagca
      121 atggctaagt ttgaaggagt ttccctttcg gaacttattc gcaccaaaac tcttgaagcc
      181 ctagaagatg aatacgatgc tcgtgtggca gatttagcct atcaagagta tttagaagac
      241 ttggaaaaag gagttgaacc cattacttgg gaagaaatga tgcatgattt aggcttgaag
      301 gatgaataat ttgtataaat tagttccaac aagacgtttt atcaagcaat tgaaaaaatt
      361 ggaccgttat acgcagaagc taattacaaa ctatttacaa accaatgttt tggaagaccc
      421 aagacgacac ggaaaggctt tggttggtaa tcgcgttggt caatggcgct atagaattgg
      481 taattatcga gttatcgtac aaattgtaga tgatgaatta gttgttgcta ctctagaagt
      541 tggtcattgg agagatattt attgaattaa agctgagtaa taacagtata gaaagtaagg
      601 atagtaaaaa tttgaataaa aaatggttat ttgcagatta ttatgatata acaattattt
      661 tacttgcttt aatctctgtt attctggtat tacttggatt tgctgagatg attgatttgg
      721 acaatccgcc ttatagtatt attgatttag taatctgggg tgtttttgta attgattata
      781 gttggcgctt ttttattact aaaagaaaat ggcgctttat tcttgaaaat atttttgatt
      841 tattgactat tctcccttta aatgctattt ttacagtgtt tcgattagga cgtatcttcc
      901 gcttagcaag gctgacaaaa ttactgaaac tgactcgttt acttagaata attggactta
      961 caggtaaatt agaaagaaaa attagtagat tcttacgaac aaatggattg atttatatct
     1021 tgtatgttaa tatctttatt gttctagtag ggagttcgat tttatccgtt gttgaagaaa
     1081 aatccttctc agatagtctt tggtgggctc ttgtgacagt aactactgtt ggttatggtg
     1141 atattgttcc agtttccctt tttgggaaga cgaattatcg agccaaagaa tattaataga
     1201 gaaacaatcc gagaagatag aagaactgca tcggatgata caagatttgc ttgaaaaaac
     1261 ataataaaaa taaagatgag acaaagtttg agttgaggag aaatcaagta agttttgaca
     1321 caatagaacg catattatca ggtataaaaa cttgatacta tgcgttttat agagaaatga
     1381 aataaaatct gaacaaatca attaggaatt taaagcattt tataacaacg ttttagagta
     1441 atggggggct atttcaactt caacctacta taatacagaa aaaaacaact ccctgataat
     1501 tcaaggagtt gtctatagtt aaattagttt ttagaagctt cttggaattc tgggtttttc
     1561 catgcttcgt caatgatagc ttgcaattct ttagcagatg cttgcatttt ttgagtttct
     1621 gcgtcgttca atgggatatt tactggacga acaataccat gtgcaccaac aacagctggt
     1681 tgaccgataa agacgttctc aactccgtat tgaccttctt ggaatactga aagtggaagt
     1741 actgcatttt catcgtcaag gattgcttta gtgatacgag caagggctac tgcgataccg
     1801 tagtatgttg cacctttttt gttgatgatt gtgtaggctg catcacgaac accttcgaac
     1861 aattcaatca attcagcttc ttgaacattt tgagtatctt taaggaattc ttcaaggttt
     1921 acaccagcga tgttagcgtg tgaccaaaca gcgaactcag agtcaccgtg ttcacccatg
     1981 atgtaggcgt gaactgaacg agcatctacg tccaattttt cagcaagtgc ttgacggaaa
     2041 cgagctgagt caagtgaagt acctgaaccg ataacgcgtt ctttagggaa accagagaat
     2101 ttccaagttg agtaagtcaa aacgtcaact gggttagcag caacaaggaa gatacctttg
     2161 aaacctgatt caacaacttg agttacgatt gatttgttga tagcaaggtt tttacctaca
     2221 aggtcaagac gagtttcacc tggtttttga ggcgcacctg cagtgatcac aacaaggtca
     2281 gcgtctgcac agtcagagta ttgagctgca tagatttttt taggtgaagt gaaggcaagg
     2341 gcgtgactaa ggtcaagcgc atcaccaaca gctttttcat gcaattgtgg aatttcgata
     2401 attccaagct cttgtgcaat tccttggtta acaagtgcaa aagcgtaaga tgaacctaca
     2461 gcaccatcac cgacaaggat aacttttttg tgttgtttag ttgaagtcat tgttttaaac
     2521 atctccttaa ttttattagg ggattttccc tagacaactt cattctatca cttttaaaaa
     2581 actttgtcac gaatatgcct tatagttctc gatgtaaacg ttttagtggt ttagaggctg
     2641 aaatagatgg gaatttatgg tataatgttg ttacttacta attgtgaaat gaggcattta
     2701 ttaatgcagg ataaaaattt agtgaatgtc aatctgacaa aggagatgaa ggcaagtttt
     2761 atcgactacg ccatgagtgt tatcgtagcg cgagctcttc ctgatgttcg agatggctta
     2821 aaacctgttc accgtcgcat tctttacgga atgaatgaat tgggtgtgac cccagacaaa
     2881 ccccataaaa aatctgctcg tattacaggg gatgtcatgg gtaaatatca cccacacggg
     2941 gattcctcta tttatgaagc catggtccgt atggctcaat ggtggagcta ccgttacatg
     3001 cttgtagatg gtcatgggaa ttttggttcc atggatggag atagtgctgc cgctcaacgt
     3061 tataccgagg cacgtatgag caagattgct ctggaaatgc ttcgtgatat caacaaaaat
     3121 acagttgatt tcgttgataa ctatgatgcc aatgaacggg aacccttggt cttgccagcg
     3181 cgttttccaa accttttggt taatggagca actggtatcg cggttgggat ggcaaccaat
     3241 attccacctc ataatctggg tgaaaccatt gatgcagtga agttggtcat ggataatcct
     3301 gaagtgacta ccaaggactt gatggaagtc ttgcctggac cagattttcc aactggtgct
     3361 cttgtcatgg ggaaatcagg tatccataag gcttatgaaa caggtaaagg ttcgattgtc
     3421 ctacgttctc gtacagagat tgaaacgact aagactggtc gtgagcgcat cgttgtaaca
     3481 gaatttcctt acatggtcaa taaaaccaag gtgcatgagc atattgttcg cttggttcag
     3541 gaaaaacgca ttgagggtat cacagcagta cgtgatgagt caaaccgtga aggtgttcga
     3601 tttgttattg aagtcaagcg cgacgcctca gccaatgtta ttctcaataa cctcttcaaa
     3661 atgacccaaa tgcaaaccaa ttttggtttc aatatgctcg ctatccaaaa tggtataccg
     3721 aaaattttgt ctcttcgtca gattttggat gcttatatcg agcaccaaaa agaagtggtt
     3781 gttcgtcgta cacgttttga taaggaaaaa gcggaagcgc gcgctcatat cttagaaggt
     3841 ctcttgattg cgctagacca tatcgacgaa gtgattcgta tcatccgtgc tagtgaaacg
     3901 gatgcggaag ctcaagctga gttgatgagc aagtttaagc tttctgaacg tcaaagtcaa
     3961 gctatccttg atatgcgtct tcgtcgtttg acaggtttgg aacgcgataa gattcaatct
     4021 gagtatgatg acctcttggc tctgattgcg gatttagcag atattcttgc taagcctgaa
     4081 cgtgtttctc aaattatcaa agacgaattg gatgaagtta aacgtaaatt ttctgataag
     4141 cgccgtacag agttgatgat tggacaggtc ttgagtctcg aggatgagga cttgattgaa
     4201 gaatcggatg tcttgattac cctttctaac agaggctaca ttaagcgttt ggatcaggac
     4261 gagttcactg ctcaaaaacg tgggggtcgt ggtgtccaag gaacgggagt gaaagatgat
     4321 gactttgttc gtgagttagt gtcaactagc acccatgatc atctgctctt cttcacaaac
     4381 aagggacgtg tctatcgtct taaaggttat gaaattcctg agtatggtcg gactgccaaa
     4441 gggctaccag tagtcaatct cttgaaattg gatgaagacg aaagtattca gacggttatc
     4501 aatgttgagt ctgatcgcag tgatgatgct tatctcttct ttacaacccg tcacggtatt
     4561 gtgaagagaa ctagtgttaa ggagtttgcc aatattcgtc aaaatggtct caaagcgctg
     4621 aatttaaagg atgaagatga gttaatcaat gtcttgttgg cagaaggaga tatggatatt
     4681 atcattggta ccaagtttgg ttatgcagtt cgctttaatc aatcagccgt tcgtggtatg
     4741 agtcgtatcg ccactggtgt gaaaggtgtt aaccttcgtg aaggagacac agttgttggt
     4801 gccagcttga ttactgatca agatgaggtt cttattatca cagaaaaagg atatggtaag
     4861 cgtacagtcg ctactgaata cccaacaaaa ggtcgtggtg gtaagggaat gcagacagct
     4921 aaaattaccg aaaaaaatgg cttgctggcc ggtcttatga ctgttcaagg ggatgaggat
     4981 ttgatgatta tcactgatac aggtgtcatg attcgaacca atcttgccaa tatttcacaa
     5041 acaggacgtg caactatggg agttaaagta atgcgcctgg atcaagatgc tcagatagtg
     5101 actttcacaa cggttgcggt ggcagaaaaa gaagaagttg ggacagaaaa cgaaacagaa
     5161 ggtgaagcat aatgtctcaa aaaaataata aaaagaaaaa caagcgaaaa aatctgctga
     5221 caaatatcct agcaggattt ctgatattac tgtcactggc tttgattttt aatactcaaa
     5281 ttcgaaatat tttcatagtc tggaatacca ataagtatca agttagccag gtatcaaaag
     5341 aaaaattaga agaaaatcag gatacagaag gcaattttga ctttgattct gtcaaagcta
     5401 tctcttcgga agctgttcta acttctcaat gggatgctca aaaattacca gttattgggg
     5461 gaattgcaat tcctgaattg gaaatgaatt tgccgatttt taaaggactt gataatgtta
     5521 atctcttcta cggagctggt acaatgaaac gcgagcaagt aatgggagaa ggaaattata
     5581 gtctagctag tcaccatatc tttggtgttg ataatgctaa taaaatgtta ttttctcctt
     5641 tagataatgc taaaaatggc atgaagattt atctaactga taaaaataaa gtttatactt
     5701 atgaaatacg tgaagtcaaa cgtgtgacac cagatcgtgt tgatgaagtt gatgatagag
     5761 atggggtcaa tgaaatcaca ttagtaacct gtgaagacct tgctgctaca gaacgtatta
     5821 ttgtcaaagg tgatttgaaa gaaacaaaag attattcaca aacatctgat gaaatcctaa
     5881 cagctttcaa tcaaccatat aaaca
//
LOCUS       DAVKQB010000082         5900 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKQB010000082 DAVKQB010000000
VERSION     DAVKQB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.082"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            109..1809
                     /locus_tag="VW528_002016"
     CDS             109..1809
                     /locus_tag="VW528_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9912774.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1802..2278
                     /gene="ilvN"
                     /locus_tag="VW528_002017"
     CDS             1802..2278
                     /gene="ilvN"
                     /locus_tag="VW528_002017"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9912775.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2344..3366
                     /gene="ilvC"
                     /locus_tag="VW528_002018"
     CDS             2344..3366
                     /gene="ilvC"
                     /locus_tag="VW528_002018"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9912776.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3473..3709
                     /locus_tag="VW528_002019"
     CDS             3473..3709
                     /locus_tag="VW528_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912777.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     SREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            3947..4303
                     /locus_tag="VW528_002020"
     CDS             3947..4303
                     /locus_tag="VW528_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9912778.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            4353..5603
                     /gene="ilvA"
                     /locus_tag="VW528_002021"
     CDS             4353..5603
                     /gene="ilvA"
                     /locus_tag="VW528_002021"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9912779.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1822 a         1000 c         1347 g         1731 t
ORIGIN      
        1 taaacgttaa aatagaaaat tcagaaaatt tcttctttta tcttgaaaaa ttttgaaaaa
       61 atggtatgat agtaacaagt tatttttaag aggaaagaaa ggggaacaat ggagaaaatc
      121 agtttagaat ctcctaagac ggggtcggac ctagttttgg aaacacttcg tgatttagga
      181 gttgatacca tctttggtta tcctggtggt gcggttttgc ctttttatga tgcgatatat
      241 aattttaaag gcattcgcca cattctaggg cgccatgagc aaggttgttt gcatgaagct
      301 gaaggttatg ccaaatcaac tggaaagttg ggtgttgccg tcgtcactag tggaccagga
      361 gcaacaaatg ccattacagg gattgcggat gccatgagcg atagcgttcc ccttttggtc
      421 tttacaggtc aggtggcgcg agcagggatt gggaaggatg cctttcagga ggcagacatc
      481 gtgggaatta ccatgccaat cactaagtac aattaccaag ttcgtgagac agctgatatt
      541 ccgcgtatca ttacggaagc tgtccatatc gcaactacag gccgtccagg gccagttgta
      601 attgacctac caaaagatat atctgcttta gaaacagact tcatttattc accagaagtg
      661 aatttaccaa gttatcagcc gactcttgag ccgaatgata tgcaaatcaa gaaaatcttg
      721 aagcaattgt ccaaggctaa aaagccagtc ttgttagctg gtggtggaat tagttatgct
      781 gaggctgcta cggaactaaa tgaatttgca gaacgctatc aaattccagt ggtaaccagt
      841 cttttgggac aaggaacgat tgcaacgagt cacccactct ttcttggaat gggaggcatg
      901 cacgggtcat tcgcagcaaa tattgccatg acggaagcgg actttatgat tagtattggt
      961 tctcgtttcg atgaccgttt gacggggaat cctaagactt tcgctaagaa tgctaaggtt
     1021 gcccacattg atattgaccc agctgagatt ggcaagatta tcagtgcaga cattcctgta
     1081 gttggagatg ctaagaaggc cttgcaaatg ttgctagcag aaccaacagt tcacaacaac
     1141 actgaaaagt ggattgagaa agtcactaaa gacaagaatc gtgttcgttc ttatgacaag
     1201 aaagagcgtg tggttcaacc gcaagcagtt attgaacgaa ttggtgaatt gacgaatgga
     1261 gatgccattg tggtaacaga cgttggtcaa caccaaatgt ggacagctca gtattatccc
     1321 taccaaaatg aacgtcagtt agtgacttca ggtggtttgg gaacaatggg ctttggaatt
     1381 ccagcagcaa tcggtgctaa aattgctaac ccagataagg aagtagtctt gtttgttggg
     1441 gatggtggtt tccaaatgac caaccaggag ttggctattt tgaatattta caaggtgcca
     1501 atcaaggtgg ttatgctgaa caatcattca cttggaatgg ttcgccagtg gcaggaatcc
     1561 ttctatgaag gcagaacatc agagtcggtc tttgataccc ttcctgattt ccaattgatg
     1621 gcgcaggctt atggtattaa aaactataag tttgataatc ctgagacctt ggctcaagac
     1681 cttgaagtca tcactgagga tgttcctatg ctaattgagg tagatatttc tcgtaaggaa
     1741 caggtgttac caatggtacc ggctggtaag agtaatcatg agatgttggg ggtgcagttc
     1801 catgcgtaga atgttaacag caaaactaca aaatcgttca ggagtcctca atcgctttac
     1861 aggtgtccta tctcgtcgtc aggttaatat tgaaagcatc tctgttggag caacagaaga
     1921 tccgaatgta tcgcgtatca ctattattat tgatgttgct tctcatgatg aagtggagca
     1981 aatcatcaaa cagctcaatc gtcagattga tgtgattcgc attcgagata ttacagacaa
     2041 gcctcatttg gagcgcgagg tgattttggt taagatgtca gcgccagctg agaagagagc
     2101 tgagatttta gcgattattc aacctttccg tgcaacagtg gtagacgtag cgccaagctc
     2161 gattaccatt cagatgacgg gaaatgcaga aaagagcgaa gccctattgc gagtcattcg
     2221 cccatacggt attcgcaata ttgctcgaac gggtgcaact ggatttaccc gcgattaaaa
     2281 atccaactta aatttattaa accagcctaa aaggcaataa ataatagaaa agagagaaaa
     2341 gctatgacag ttcaaatgga atatgaaaaa gatgttaaag tagcagcact tgacggtaaa
     2401 aaaatcgccg ttatcggtta tggttcacaa ggacatgcgc atgctcaaaa cttgcgtgat
     2461 tcaggtcgtg acgttattat cggtgtacgt ccaggtaaat cttttgataa agcaaaagaa
     2521 gatggatttg atacttacac agtagcagaa gctactaagt tggctgatgt tatcatgatc
     2581 ttggcgccag acgaaattca acaagaattg tacgaagcag aaatcgctcc aaacttggaa
     2641 gctggaaacg cagttggatt tgcccatggt ttcaacatcc actttgaatt tatcaaagtt
     2701 cctgcggatg tagatgtctt catgtgtgct cctaaaggac caggacactt ggtacgtcgt
     2761 acttacgaag aaggatttgg tgttccagct ctttatgcag tataccaaga tgcaacagga
     2821 aatgctaaaa acattgctat ggactggtgt aaaggtgttg gagcggctcg tgtaggtctt
     2881 cttgaaacaa cttacaaaga agaaactgaa gaagatttgt ttggtgaaca agctgtactt
     2941 tgtggtggtt tgactgccct tatcgaagca ggtttcgaag tcttgacaga agcaggttac
     3001 gctccagaat tggcttactt tgaagttctt cacgaaatga aattgatcgt tgacttgatc
     3061 tacgaaggtg gattcaagaa aatgcgtcaa tctatttcaa acactgctga atacggtgac
     3121 tatgtatcag gtccacgtgt aatcactgaa caagttaaag aaaatatgaa ggctgtcttg
     3181 gcagacatcc aaaatggtaa atttgcaaat gactttgtaa atgactataa agctggacgt
     3241 ccaaaattga ctgcttaccg tgaacaagca gctaaccttg aaattgaaaa agttggtgca
     3301 gaattgcgta aagcaatgcc attcgttggt aaaaacgacg atgatgcatt caaaatctat
     3361 aactaattag aaatatatag cgctggagat gattttatga aaaagattat gagaaaaact
     3421 gcatcgttat tattggttct agttgtataa tgtaattaca ccgtcggtaa tagtgctagc
     3481 agaccaaaat aaagcagatt ggtcgtatga tgaaaatgct gtaattaaca tttatgatga
     3541 tgctaatttt gaagatggta ggttgcatat gaactttgaa caattcttca aattggcaca
     3601 aatatctaga gaagaaggtc ttgaaattca ttctccgttt gagagagctg gtgcgactaa
     3661 atctgctcgt tatatagcga aatggatttt gagaaataaa aaacattaac aaatatagtt
     3721 ggtaaatcat taggacctaa atcagctgtt agattcggag aagctttatc ctatattgaa
     3781 ggtcctcttc gcagaataaa tgagacgata gatggcggtt tatatcaaat agagcaaatt
     3841 attgcatctg gattgaaaga atcgggttta aatgactgga ctgcgaaaac tttagcttca
     3901 gctattcgtg ggatattaga tgtacttatt taggggttga aatcatatga atattaccaa
     3961 tttgttttct atcaagacag gatgtgatga aactgatagg caactgcaaa aactattttt
     4021 tcagttggat ttacaattgg gagaattgac agatcaacta agaaaattag attctaattt
     4081 tgttcctcgt agtcaatttg tagacacgtt ggatttgaat gatgtagaat ataaagaaat
     4141 tttaaactat tttatcttcc atcgtaatga tagtgaagaa agtttggtag aatggttata
     4201 tgattggatt tccacaaatc gttatgaact tcctaaagag ttttcgattc gtatggctca
     4261 taaataccat gaaagtgtta ctgaagtttt cggagatgaa taactaaaaa acagtcatta
     4321 gtgactgttt tttatagaaa aagaggtttt atatgttaag ttcaaaagat ataatcaagg
     4381 ctcacaaggt cttgaacggt gtggttgtga atactccact ggattacgat cattatttat
     4441 cggagaagta tggtgctaag atttatttga aaaaagaaaa tgcccagcgt gttcgctcct
     4501 ttaaaattcg tggtgcctat tatgccattt cccagctcag caaggaagaa cgtgaacgtg
     4561 gggtagtctg cgcttctgcg ggaaatcatg cgcagggagt agcctatact tgtaatgaaa
     4621 tgaaaattcc tgctactatc tttatgccca ttactacgcc acaacaaaag attggtcagg
     4681 ttcgcttttt tggtggggat tttgtaacta ttaaactagt tggagatacc tttgatgcct
     4741 cagccaaagc agctcaagaa tttacagtct ctgaaaatcg tacctttatt gatccttttg
     4801 atgatgctca tgttcaagca ggtcaaggaa cagttgctta tgagatttta gaagaagctc
     4861 gaaaagaatc gattgatttt gatgctgtct tggttcctgt tggtggtggc ggtctcattg
     4921 ccggggtttc tacctatatc aaggaaacaa gtccagagat tgaggttatc ggagtagagg
     4981 cgaatggagc gcgttccatg aaagctgcct ttgaggctgg aggtccagtc aaactcaagg
     5041 aaattgataa atttgcggat gggattgctg tgcaaaaggt aggtcagttg acctatgaag
     5101 caactcgtca acatattcaa actttggtag gtgtcgatga gggattgatt tctgaaacct
     5161 tgattgacct ttactctaag caagggatag tcgcagaacc tgctggagca gctagtatcg
     5221 cctctttaga ggttttagct gaatatatta aggggaaaac catttgttgt atcatttctg
     5281 gaggaaataa tgatatcaac cgtatgccag aaatggaaga gcgtgccttg atttatgatg
     5341 gtatcaaaca ttactttgtg gtcaatttcc cacaacgtcc aggagctttg cgtgagtttg
     5401 taaatgatat cctggggcca aatgatgata tcacacgttt tgagtatatc aaacgagcta
     5461 gcaagggaac aggcccagta ttaattggga tcgctttagc agataagcat gattatgcag
     5521 gtttgattcg tagaatggaa ggttttgatc cagcttatat taacttaaat ggtaatgaaa
     5581 cgctttataa tatgcttgtc tgaggactaa taaaaaaata tcatattatt tgtacaactg
     5641 atgtatatgt gctataatga agatgaagaa aggggacgat tcctatggtt ttagaaaact
     5701 tatcgtgcaa ttcttacttc tgttcattat agcacttatg atgtttggct cagatttcca
     5761 tccttcattt tgatttccta tctattgaca agcatagtca cactgtcttt aatactcttc
     5821 gaaaatctct tcaaaccacg ttagctctat ctgcaacctc aaaacagtgt tttgagcaac
     5881 ttgcggctag cttcctagtt
//
LOCUS       DAVKQB010000083         5779 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKQB010000083 DAVKQB010000000
VERSION     DAVKQB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.083"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..237)
                     /locus_tag="VW528_002022"
     CDS             complement(<1..237)
                     /locus_tag="VW528_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912780.1"
                     /translation="MKILNIELANVEQTDLGFEHWVDVTYQVPILKNEYTVKLLLLME
                     CRIEDQEVIEYLVSTWKYRDLVLHSVRMYEIEKSE"
     gene            complement(469..1425)
                     /locus_tag="VW528_002023"
     CDS             complement(469..1425)
                     /locus_tag="VW528_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9912781.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNAETYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     SWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPDGLITVK"
     gene            complement(1429..1761)
                     /locus_tag="VW528_002024"
     CDS             complement(1429..1761)
                     /locus_tag="VW528_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU9912782.1"
                     /translation="MQQITEIITNGAISILVVLAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARTKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            complement(1765..2064)
                     /locus_tag="VW528_002025"
     CDS             complement(1765..2064)
                     /locus_tag="VW528_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912783.1"
                     /translation="MPIEEVEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHTVGDIQKEMVRMNDRMEEIEKGE"
     gene            complement(2073..2423)
                     /locus_tag="VW528_002026"
     CDS             complement(2073..2423)
                     /locus_tag="VW528_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912784.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
     gene            complement(2426..2629)
                     /locus_tag="VW528_002027"
     CDS             complement(2426..2629)
                     /locus_tag="VW528_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912785.1"
                     /translation="MNTEQLNQALRMTISEISTTSTNSMITSNILSIQLDEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(2610..3991)
                     /locus_tag="VW528_002028"
                     /pseudo
     CDS             complement(2610..3991)
                     /locus_tag="VW528_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
BASE COUNT         1473 a         1345 c         1035 g         1926 t
ORIGIN      
        1 ctcactcttt tctatctcat acatccttac cgaatgcaac acgagatcac gatacttcca
       61 agtgctgacc agatactcaa taacctcttg gtcctctatc ctgcattcca taagtaataa
      121 tagtttgacc gtgtattcat tcttcaaaat cggaacctga taagtcacat ctacccaatg
      181 ctcaaaacct aagtctgtct gctctacatt tgctagttca atatttaaaa tcttcatttt
      241 tattcctcct acttatctat tcgtaaaaga agataaaaag ttatgaaaaa atcattactt
      301 ttttaattca gacagtactt tcagagcaat tttgttaatg tcaacaaaat tggcaaccaa
      361 gcgatttaat actatttgtt gacgccaaca agtcaatttt cagacaaaca aaaaaaccgc
      421 aagcctgagc ctgcggtgaa agaacaattt agaaagtttc ctttctattt atttaactgt
      481 aatcaagcca tctggctcta ctgtgaagtc tggcttatct gccagtgttc cgtctggttt
      541 gaggtagtac cagcctgttc cgtccgctga ctggataaag gcatttgata ccattgcgcc
      601 ttctttagcg tctaagtagt accatgtgtc cttgtacttg acccagcctg tcttcatggc
      661 accttctaca tcaaaatagt accacttctc agcgattttc ttccagcctg tggccatttc
      721 gcctgattgg tcaaagtagt accaattacc gtctgtgtgc ttcttccagc tgtctgaaag
      781 catatagcct gagccatcga aataatacca ggtaccgttg attttctcaa acttatcttt
      841 tggataagag ccgtctgagt gtacgtacca gtagccagtg ccatttttct gccagcctgt
      901 tgcagcgctc aagccgtttt cgatgtcttg cttaaactgt tcacggctaa tgccccaact
      961 tgcaagatat ggatatggat ccacatggtc tgagtggttg tttggttggt tattggtaca
     1021 gtattcatgc gtcttgatac ctgccaagtc gtctgtatca agagtcttcg gtaagcctgc
     1081 ttcgtccgct agattgcgta gcaattcgat atagaggcga tagtcagcca taaactcttc
     1141 cttagttgaa tggctttcaa tcagttcaac cgctgcatag gtctcagcat tccaaccgcc
     1201 cccaacatcc caacttccgt tgttcacagg acctacttgc atgacacggc cgtttccgac
     1261 aacatgtgaa aagaacccta gttcagggtc ctttctatag tggtaatcag cttcattttg
     1321 agcggttgag ttacggttgc ctgttgagtg agcatgtact tgtcgataag gctgcacccc
     1381 aacctgtggc aagcctgtac gtagtctgtt tctatcgata tccattccct atcgtccttt
     1441 ccatgcgtca ttcatctgct tcactgctga ctctacgaag gtgtctaagt ctttgtcagt
     1501 catgctaata ttgtattttg taagctcagc acggacttta gtgcgagcct gtgccagttt
     1561 ttcatctccc ttgtagccag tttcagcagc tacctgctcc acggcattaa ctgcgttttt
     1621 ggccaagatt tcaacaatct tgatggtctt ttctccacct ttttgaacca ggtagtcctt
     1681 gactgcccta actacaaccc ctgccaaaac gactaggatg ctgattgctc cattagtaat
     1741 gatttcagta atttgttgca tttgttattc tcctttttcg atttcttcca tgcggtcgtt
     1801 catgcgaacc atttcttttt gaatgtctcc gaccgtgtga gtgattgtgg ttaattctgt
     1861 agtggtcttt tctaggtgag tcatcaaacg ctcttctcgt ctattagagt cggcctttga
     1921 ttgctcgtgc aaatccataa tcttcttctc tcgcttgtcc gaagtcttga taagatatcg
     1981 aatgataata aaaaaaagca agataaacaa aatcgcccaa gctacctgac tttgagcgat
     2041 tttttcaact tcttcaattg gcatacaacc tcctattctt taggttctac cgttggaacc
     2101 gtccagtcag gattgccctc tgcatcaaat ttcatgatat agaattcatg attcaacaga
     2161 acggctacgt tgattgttgc gattgtacca ccccactgat tgaacgccca aacggtttca
     2221 acatccttga attggcgacg gccatttaca atcacaggac gtttttgaac gtcacgatac
     2281 atatagaagt catcgcttac attcttgcaa cgaatgaact ctccattttc tttcatgtag
     2341 cgcaaagcac tcgcaagatc aaatggttct gtgatttttg taaggtctag taagttatct
     2401 gtgttttgaa ttgtttctgc catgtctatt ctcctttgtc tgctggttta gtttgttcat
     2461 caagcagagc ttccagctca tccactcgtg cttgaagtct ttgattttct tccctttgct
     2521 catccaactg aatactcaag atattacttg taatcatcga atttgttgaa gttgttgata
     2581 tttcactaat tgtcattcgt aaggcttggt taagctgttc tgtgttcatt ttctaagttc
     2641 tccaatctgt gtgttcgttt tctattttca agagcaaact cctgaattgc tttaagtgcg
     2701 atattggtca acatgttgtt attcatgcta ttgttttctc cattttttct attttttgat
     2761 ttaattcttg aatagccttg attaagtaag gaactaaagc ggtatagtca atatgtagat
     2821 agccatctgg attctcagga tctcgtgaga caattcttgg aacgatggtt tcagcctctt
     2881 gagctattag accaatctcc tcatgtttct tattttcgat gaaatcaaat gcaaccattc
     2941 ttaatctgtt gattttatcc aaggctttca cagctgtatc tgtgatgttc tcttttaagc
     3001 gtctatctga tttttgttcc atccaatact tcaagctacc gctaccgacc tgattccacc
     3061 aaacaaccgc attccttccg cctttgggat tccaaccatc accaagcaca tctttacttc
     3121 caagttcgat accatttgaa aacacaggag aacgagaaaa agtagtattc ccatagaagt
     3181 ttgctctcga tgaattcgaa aaatccactt gatcataaaa accgacttca ttcctacagt
     3241 acattttccc atcagtattg acgttccatg ctttaggtcc ggcatagttc caattatttc
     3301 cccagttcgc ccagaaggct gtccggactc catacccggc accattcccc ataccaacag
     3361 agaactgatt gacacctgaa atccagcgac cgccaccctg gtcaaattga ccaagtgtga
     3421 atccaccgat tcggccttga taggcttcta ggaaggttga gctagaaatg acggactcaa
     3481 ccttagtaga gaagatacgt ttagatatca gttggtcaat aaaagcatca gttgcagtta
     3541 attttttaat aagcgcattg tcaactttca acttctcagc agttaccgct tcagcttcta
     3601 atatcgtagt cgtgaccgaa ccagcttcaa aattggccgt tttgagctta tcaaccatgg
     3661 cagacttgat gactgctctg tcaatcaggg tctctccagt gatgtgggtc aatttcccaa
     3721 ctaagcggtt atgtccattg gcgccaagat tgattccaga gatgatatct ccagccgagt
     3781 tgatgttttc aactacccat gagccagcta gttgagtcat ttttgtttgc gttgcttcaa
     3841 gcttcttatt cgcatctgcg actgcatctt ctggatgtgg ttgccatgtt ctaggtttat
     3901 aacctttgta caagtcaact tctgtaatat acaaatcagc tgttcctgat gatgagccat
     3961 tgttatcaaa acgaatgtaa gcattatcca tttctccgga attaaaagtt actgagacat
     4021 cttcgcatct agaggtagat agtttcttgc tgctaacaac tttcttaacg attgtgaatc
     4081 catcgctctc gcctgctctt cgtcccaaaa tataaacatc atagtttgct agagcactgt
     4141 tgttaaatcc tctaaaattc agtacatagt cagtatttcg ttcaagatta aaacggtgac
     4201 tatacaaaaa attttcgttt ttagttgcat tacttaaacg cataaggtct ttctgcccgt
     4261 tgtgataaaa gctatgctta accaatcttc ctaaattttg agttgagccc cattcattcg
     4321 tagaattttt aaaatcacta ttcttaatga ggttagggcc gcttacacta tatttcccaa
     4381 cctcaacctg aaacagttga ttggtcagag ccatgcgagc aaccttatcc gcaattccat
     4441 tttcagtatt gcccaaaatc cgctcgtaaa gtttactggt ttccttaaca cgctgaaagt
     4501 cagtagtctc tacttttcgc gctagttgat tggtcacatt cgcaaattga ctatcagcat
     4561 tcgctttgtt tgccgaaacc tgatcagata ttctacccat ttgtcgttca gcattatcct
     4621 tgtttgtagc gacctgagtc tttaaatttg aaatctgatt atctgcgcct tgtttattac
     4681 tgtttatccg atttgaaaga tttgaaatct gagtagtggt tccttgctca ctgcttgtaa
     4741 gtctatttga tagaccactg atttgaccgc ccacatcttg cttataagta gttatctgac
     4801 ttgaaatatc cgtgaactta ccatctacag attgacgata gctagcgatt tgactagcga
     4861 tgtctttatg cgcactagtt ttaacagctt caatcctctg attgataccc ttaacatctt
     4921 cttgataagt agccttacca acgaaatcac gattgaccag ctcacggact gctgtcgctt
     4981 gtctcgtgct ctcctcacga gtatagcgct gtagggcttc ctgtcgctga ccgtctttat
     5041 ttacatattc ctgaatagct gataagtcgg ttcgcaagcc ctgagctgtc cgctcaaagg
     5101 tagtcttagc ttcagtaatg agaccatcag cgtcctcagg cgcaggactc cagtccgtcg
     5161 ccacactacc gatttcaacc ttgattcctg ttacccaagc tgtaccgctt gtagcacctt
     5221 caagattgaa tcgcaatgat gtcttcaatt gatcaaaatt tgttttttca gagtagtcat
     5281 aagtgaatgt aatatatttc caatctgccg aacctttata cataccaagc gtagcataat
     5341 ctggaccact ctgtactccg gtctcactat tttttctaaa aagataatgt ttgaagcaat
     5401 taaatacatt ccaaaaattt cgaccttgga ctacattttc gtacttgatc caagcgctaa
     5461 aagtaacttt ttggtacaac cttgagctga aatctggttc aaggttgaac gttaaagtag
     5521 agttgttctc tagcctatag cattcttttt gacctgtgac gtggttttca ggtaattttt
     5581 caattacagc tccaaccgtc ttggatttta tccatagatt ccggcctccc accttcattt
     5641 ttgaaaattc ttcacgcaat ttcccggctt cagatacaac taaagtctta tctgctttat
     5701 ccttggttgc gttcaagatt tcctgacgga tagagccggc tcgcacctca aattcagcct
     5761 gactcaactt ctgatttag
//
LOCUS       DAVKQB010000084         5405 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKQB010000084 DAVKQB010000000
VERSION     DAVKQB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.084"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..800
                     /locus_tag="VW528_002029"
     CDS             <1..800
                     /locus_tag="VW528_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9912786.1"
                     /translation="KGYFLRIEDVFNTKSELIPERVSQDIKFDKKIELKDIWYKYGLF
                     DDYVLKGINVTIKKGETVAIVGESGSGKSTLAKILLGLLEPNIGSIEVDGVEKEEIGQ
                     TLYRKIFGAVLQNSTLSYGTLRENLTFGHFVSDEELMTNLNSIGLSNVVKSLPLGLET
                     IIAEEGNNFSGGQQQMILLARCLLSKPSVVVLDEATSSLDNLSQQITTSYLSEIGTTK
                     ILIAHRLDTIKSADKILVMHNGEIVEIGTHRELLELGGIYKQLYSNN"
     gene            824..2562
                     /locus_tag="VW528_002030"
                     /pseudo
     CDS             824..2562
                     /locus_tag="VW528_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            2613..3380
                     /locus_tag="VW528_002031"
     CDS             2613..3380
                     /locus_tag="VW528_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9912787.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            3382..4014
                     /locus_tag="VW528_002032"
     CDS             3382..4014
                     /locus_tag="VW528_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912788.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(4960..5373)
                     /gene="ndk"
                     /locus_tag="VW528_002033"
     CDS             complement(4960..5373)
                     /gene="ndk"
                     /locus_tag="VW528_002033"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9912789.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
BASE COUNT         1881 a          798 c         1018 g         1708 t
ORIGIN      
        1 taaagggata ttttttaaga atagaggatg tgtttaatac taaatccgaa ttaattccag
       61 aaagagtcag tcaagatata aaatttgata aaaaaataga attaaaagat atttggtata
      121 aatatggatt atttgatgat tatgttttga aaggaataaa tgttactatt aaaaaaggag
      181 aaactgttgc tattgttgga gaatcaggtt caggtaagag tacattagct aaaattttat
      241 taggtttatt agaacctaat attggttcaa tagaagttga tggagtagaa aaagaagaaa
      301 ttggtcaaac attgtataga aagatttttg gagcagtgtt acaaaattca accctaagtt
      361 atggtacctt aagagagaat ttgacatttg gacactttgt ttcagatgaa gaattaatga
      421 caaatctaaa ttcaattggt cttagcaatg tagttaaatc tttacctctt ggattagaga
      481 caatcatcgc tgaagaaggt aataactttt ctggagggca gcagcaaatg atacttttag
      541 ctcgttgtct tttgtcgaaa ccttcggtag ttgttttgga cgaagcaaca agtagtttag
      601 ataatttatc tcaacaaatt acaacttctt acttaagtga aatcggtacc actaagattt
      661 taattgccca tcgactagat actatcaagt ctgcagataa gatcttagta atgcataatg
      721 gtgaaattgt agagattggg acccatagag aacttcttga actaggaggc atttataagc
      781 aattgtattc aaataattag tttttgatta aaagggtaaa tttatgaaga ttatgaaaaa
      841 aaaatattgg actttagcga tattattctt ttgtttgttc aataattctg ttactgctca
      901 agaaatacct aaaaatcttg atggcaatat aactcacact cagactagcg aaagtttttc
      961 tgaatctgat gaaaaacagg ttgactattc taataaaaat caagaagaag tagaccaaaa
     1021 taaatttcgt attcaaatcg ataagacaga attatttgta acaacagata aacatttaga
     1081 aaaaaactgt tgtaaattgg aacttgaacc acaaataaat aacgatattg ttaactctga
     1141 aagtaataat ttactaggcg aagataattt agataataaa attaaggaaa atgtttctca
     1201 tctagataat agaggaggaa atatagagca tgacaaagat aacttagaat cgtcgattgt
     1261 aagaaaatat gaatgggata tagataaagt tactggtgga ggcgaaagtt ataaattata
     1321 ttctaaaagt aattctaaag tttcaattgc tattttagat tcaggagtcg atttacaaaa
     1381 tactggatta ctgaaaaatc tttcaaatca ctcaaaaaac tatgtcccca ataaaggata
     1441 tttaggaaaa gaggagggag aggaaggaat aatatcagat attcaagata gattaggtca
     1501 tggtacggct gttgtagctc aaattgtagg ggatgacaat attaatggag taaatcctca
     1561 cgttaatatt aacgtctata gaatatttgg taagtcgtca gctagtccag attggattgt
     1621 aaaagcaatt tttgatgctg tagatgatgg caatgatatt atcaatctta gtactggaca
     1681 atatttaatg attgatggag aatatgagga cggaacaaat gattttgaaa catttttgaa
     1741 gtataaaaag gctattgatt acgcgaatca aaaaggagta attatagtag ctgcattagg
     1801 gaatgactcc ctaaatgtat caaatcagtc agatttattg aaacttatta gttcacgcaa
     1861 aaaagtaaga aaaccaggat tagtagttga tgttccaagt tatttctcat ctacaatttc
     1921 ggtcggaggc atagatcgct taggtaattt atcagatttt agcaataaag gggattctga
     1981 tgcaatatat gcgcctgcag gctcaacatt atctctttca gaattaggac ttaataactt
     2041 tattaatgca gaaaaatata aagaagattg gattttttcg gcaacactag gaggatatac
     2101 gtatctttat ggaaactcat ttgctgctcc taaagtttct ggtgcgattg caatgattat
     2161 tgataaatac aaattaaaag atcagcccta taattatatg tttgtaaaaa aattctggaa
     2221 gaaacattac cagtaaaaaa tggtataaaa gtgttaaata taccaaacgt attgagatat
     2281 gatttgaata tgttacaatt agaatataaa aatgaacaaa gttgggatag tttcatagat
     2341 aatgttaatt taattgagtt ggaagagaga attcaaacta ctattggaat taaacaaata
     2401 aacacacaca atattattac tattgcccga gaagggtact ctcaaaatta tttacctaac
     2461 acttcagaaa atacatataa ttcattacaa gtcagtttag ttggagtatt actacttttt
     2521 ataagtatgg taaatatttt atgggctaaa aaaagtaaat gaaaataaaa tttggagccc
     2581 tctgaaaaag taagtcctac agttcaacta aaatgagtca aaagatgaat caccttgatg
     2641 taggggagtt tgtcttattg ctgcctgaac acctccgttc agaggaagaa cattataaat
     2701 ctgtttttga agacgactta accagtcgca tatctagtca agatgaacga cagcaaatga
     2761 ctgctacggt aggttattta gaatcaggtc aggatcgttt tgtgtataat acgaccccta
     2821 tttcttacca gcagtttttg aaagatccaa tcatcattgt tataacaccc caatcaactg
     2881 gtccacagtc cattttgttt tggatagacg cagtacagaa ctacgttctc tttaatcaat
     2941 tgtctgatgc ccaggagctt atccagagac aaggcattga aaattgggtc tcagaaatgc
     3001 aaacaggtta ccacaactac atcacattat tggataatat ccagagggaa cgttgggtaa
     3061 tgctagcagg agctgtgctt gggattgcaa cttcaatctt gttgtttaac actatgaata
     3121 ggctctactt tgaagaattt agacgtgcca tttttatcaa acgcattgca ggtctcaggt
     3181 tcttagaaat ccatcgcact tatctctttg ctcaactggg tgtgttttta ctgggatttg
     3241 ttgcgagtgt atttcttcag gtagagatag gagttgcttt cttagtcttg ttactcttta
     3301 ctggtctatc tcttttacag ttacatgtcc aaatgcagaa agaaaacaag atgtccatgc
     3361 ttgttttgaa gggaggttaa tatgattgaa cttaaacagg tgagtaaatc ttttggagaa
     3421 cgagagttat tttcgaatct ttcaatgaca tttgaggctg gaaaagtcta tgccttaatt
     3481 ggttcaagtg gtagcggaaa aacaaccttg atgaacatga ttgggaaatt agaaccttat
     3541 gatgggacga ttttttaccg aggtaaagac ttggccaatt ataaatcaag tgattttttc
     3601 cgtcacgaat tgggctacct cttccagaac tttggcttaa ttgaaaacca aagtattgaa
     3661 gaaaacctta agctaggtct cattggtcaa aagttgagtc ggtcggaaca gcggttgagg
     3721 cagaagcagg ctttagaaca ggtcggcctg gtttatcttg acctagataa gcgcatcttt
     3781 gagttatcgg gcggagaatc gcaacgggtt gccttggcaa aaattatctt aaagaatcca
     3841 ccctttattc tggcagatga gccaacagct tcaatagacc cagcaacctc tcagttgatt
     3901 atggagattt tgctatctct tcgagatgat aataggctaa tcattatcgc aacacataat
     3961 ccggcaattt gggagatggc tgatgaagtg ttcacgatgg atcatctgaa ataaaaatcc
     4021 ttgtttttaa ttgcacgatg agttactgaa atattatcat gaatcaagaa ttggagttaa
     4081 tttagaattg tacttaattt agaattgtac tttattaata ttgaggtaac tttttcttga
     4141 taaaggaaga aataatggag aggaagttag aatgaaaaaa ttcgacaatt atattattga
     4201 gaagccttgc gattctaatt cagataaact gcaaaaaatc ttaataattg aaagtttggt
     4261 agatgatatt ttgcaatttt ctctcagaat caataatagt gtaggagaga ttttcctcct
     4321 acaaccgttt taaaagaaaa ctatctttat tccatgttat tttgaggaag atattgtgaa
     4381 agtcaaagat gatgataaag ttgagtggaa tttgttagaa tttcaaaaat ttagagcatt
     4441 tttggcttag taatctgtgt tgaaggctca aaacctatgg taaaaaagta gctttgaaaa
     4501 cgtattgcct ccaaagattt agttaaataa tgatttaaca caaaaagaaa ttattgaagt
     4561 tctggaaaga tgttgtttca gtattgagaa aaggtgggaa aaacttgcga ttttcacaga
     4621 gaaaggaaga aaaagtatag aaatatagtc aattgaaaca agaacaggat aaaagaacct
     4681 tttgtgccat atttttctcc tttcgcttta caattggatt gaacaccttt attgtatcgc
     4741 gtatggagtt tttttggtat aaccttcgac gcacacccgc atagcgggtg ttttttttgt
     4801 ctcgcaccta acggagcgag acaaactaat agtcacttaa tcaaaaaacg caccatatca
     4861 aaaactaaaa agtttgatat catgcgtcat gtcttaaact aattgactat actttctatt
     4921 caaatgagct tttaaccaat tgattgagcc aatccactct taaaaccaaa gagcaatttc
     4981 tcgcttagct gactcttctg aatctgaacc atgtacaaca ttttggataa tctcattttc
     5041 tccagcagct tttgcaaaat cacctcgaat agtgcctggt aaagcttctt ctggacgagt
     5101 tgcacccatc atggtccgcc aagtttcgat tactttggga ccagaaatga cacccacaag
     5161 aactggacct gaagtcatga attcacgaat cggtgggtaa aaactctgac caaccaagtc
     5221 ctgatagtgc tggtcaatca actcttctga aacctgtgaa cgaaactcca atttttcgat
     5281 tgtaaatcca cgttgttcga tgcgctttaa cacttcaccc actagccctc tttttacacc
     5341 atctggtttg atgataaaga atgtttgttc catacccgtc tcctttgtca gcttctttct
     5401 tttat
//
LOCUS       DAVKQB010000085         5404 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKQB010000085 DAVKQB010000000
VERSION     DAVKQB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.085"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            44..1798
                     /locus_tag="VW528_002034"
     CDS             44..1798
                     /locus_tag="VW528_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912790.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNINNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1782..3503
                     /locus_tag="VW528_002035"
     CDS             1782..3503
                     /locus_tag="VW528_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912791.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDVK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            3514..4098
                     /locus_tag="VW528_002036"
     CDS             3514..4098
                     /locus_tag="VW528_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9912792.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            4098..4793
                     /locus_tag="VW528_002037"
     CDS             4098..4793
                     /locus_tag="VW528_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9912793.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKIAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            4781..>5404
                     /locus_tag="VW528_002038"
     CDS             4781..>5404
                     /locus_tag="VW528_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912794.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDR"
BASE COUNT         1930 a          710 c         1016 g         1748 t
ORIGIN      
        1 gaatttatat ctaaaaggga tattaaagaa aggagatatg cttatgaaga tttacaaaaa
       61 actatttgct tatgtccaag ataagaaata tcttggggtt ttggccataa ttttttctgc
      121 tatatctgct ctacttacag tatatggata ttatttaatc tacaaatttc tagataagtt
      181 aataattaat tcaaatttat ccggtgcaga gagtatagca ttaaaatctg ttattacact
      241 aacaagtgga gcgatatttt attttgtctc aggaatgttt tcacacatct tgggatttag
      301 gcttgaaaca aatttaagaa aaaggggaat tgatggctta gagaaagcaa gttttaggtt
      361 ctttgactta aatccatctg gtcaaataag aaagatcata gatgataatg cagcacaaac
      421 tcatcaggtg gtagcacaca tgattcccga tagttctcag gcaataatca cacccgtact
      481 tgtacttgca cttggcttta tagtaagtat aagagttggc ataattttgc ttgctcttac
      541 tataattggc ggcttaattt taggggcaat gatgggcgag caagaattta tgaagatata
      601 ccaagaatcc ctatctaaac taagtgctga aactgttgag tacgtgagag gaatgcaagt
      661 tgtaaaaata tttaaagcaa atgtagagtc ttttaaaagc ttttataagg cgataaaaga
      721 ttactcaaag tatgcttatg attattccct atcttgtaaa aggccttatg ttttgtatca
      781 atggttattt tttggactga ttgcaatttt aattattcct atagtttatt ttatgactag
      841 cttagctagc gcaaaggtga ttttacttga gcttatcatg attttatttt tatcaggagt
      901 tctctttgtt tcattcatga gaatgatgtg gtactccatg tatatttctc aaggaaatta
      961 tgcagtagat actttagagg cgctttacga agatatgcaa aaagacaaat tagtgcatgg
     1021 taatatcaat aattttaaaa actataatat agaatttgag aatgttagct ttgcttataa
     1081 tgataaagct gtcattgaaa atttatcctt taatttagaa gaaggaaagt cctacgcact
     1141 tgtcggttca tctggatcag gcaaatcaac agtagcaaaa cttatatcag gtttttataa
     1201 tgttaataaa ggaagcataa agataggcgg gatagcaata agtgaatatt ctgacgaagc
     1261 cttaattaaa gccatttcct ttgtttttca agattcaaaa ttattcaaga agagcattta
     1321 tgataatgta gctttagcta ataaagatgc gacgaaagat gacgttatga gagccttaaa
     1381 attagcagga tgcgatttaa tattagacaa attcccagaa agagaaaata caatcatagg
     1441 ctcaaaaggt gtttatttat ccggtggaga aaaacaaaga attgcaattg ctagagcaat
     1501 tttaaaggat tccaaaatta ttattatgga tgaagcatca gcatctattg acccagataa
     1561 cgagtttgaa ttgcaaaaag cttttaaaaa tcttatgaag gataaaacag ttatcatgat
     1621 tgcacacagg ctatctacaa ttaaagacct tgatgaaatt attgtcatgg atagtggaaa
     1681 aattatagaa agagggtctg acaaagaatt aatgtcaaaa gatacaaggt ataagagcct
     1741 gcaagagatg tttaacagtg cgaatgaatg gagggtttca aatgaaagag ttttataaaa
     1801 aaagatttgc tcttacagat ggaggagcaa gaaatttaag taaagcaaca ctggcttcat
     1861 ttttcgttta ttgtataaac atgcttcctg ccatattact tatgattttt gctcaggaag
     1921 ttttggaaaa tatgggcaaa agcaatggct tttatatagt attctcagtt ttgactttga
     1981 tagcaatgta tattttgctt tctatcgaat acgataaatt atataacaca acctatcaag
     2041 aaagtgcaga tttaagaata aggacagcgg agaatttatc aaaattacct ctatcttact
     2101 tttctaaaca tgacatttcc gacatttcac aaacaatcat ggctgatatt gaaggcatag
     2161 agcatgcaat gagccactca ataccaaagg tgggcggcat ggtactgttt ttcccattaa
     2221 tatctgtaat gatgctagcg ggcaatgtca agatgggttt agctgtaatt attccatcta
     2281 ttttaagctt tatatttata cctttatcta aaaaatatca ggttaatgga cagaatagat
     2341 attatgatgt cttaagaaaa aactcagaaa gctttcaaga aaatatcgaa atgcaaatgg
     2401 agattaaagc atataattta tcgaaggata ttaaagatga cttatataaa aaaatggaag
     2461 atagtgagag agtacactta aaggcggaag taactacaat tttaactttg tctatatctt
     2521 caatatttag ctttatatct cttgctgttg tgatatttgt cggcgtaaat ctaattatta
     2581 ataaagagat aaattctctc taccttatag gatatttact agctgctatg aagataaaag
     2641 actctttaga tgcatctaaa gagggcttga tggaaatatt ttatttatcg cccaaaatag
     2701 aaagattaaa agaaattcaa aatcaagatt tacaagaagg cgatgactat agcttaaaaa
     2761 aatttgatat tgatctaaaa gatgttgagt ttgcctacaa taaagacgca aaagttttaa
     2821 atggtgtaag ttttaaagct aagcagggag aggtcactgc tttggtaggt gcaagtggct
     2881 gcggtaaaac aactatcttg aaacttatat caagacttta tgattatgac aagggacaaa
     2941 tcttaatcga tgtcaaagat ataaaagaaa tatcaacaga atctcttttt gataaggtct
     3001 ctatagtttt ccaagatgtg gttctcttta atcaaagcgt tatggaaaat attagaatcg
     3061 gaaagcaaga tgcaagtgac gaagaggtta aaagagcagc aaaacttgca aattgcacag
     3121 attttataga aaaaatggat aaaggtttcg atacagttat tggtgagaac ggagctgagc
     3181 tatcaggagg agaaagacaa agattatcaa tagccagagc cttcttaaaa gatgcgccga
     3241 tattgatctt agatgagata acagcaagcc ttgatgttaa caacgagaaa aagattcaag
     3301 agtctttaaa taatttagtt aaagataaaa ctgttgtaat catttcacat agaatgaaat
     3361 ccatagaaaa tgcagacaag atagtagttc ttcaaaacgg aagagtagaa agcgaaggta
     3421 agcatgaaga gcttttacaa aaatcaaaaa tttacaaaaa tttaatagaa aagacaaaaa
     3481 tggcagaaga atttatttat taggaggact acaatggata ataaaaaatt aaaagtaaaa
     3541 gatttagtaa gtatcggtgt ttttggcgta atttattttg ccttcatgtt tggagttggt
     3601 atgatgggct tgattccaat attgttctta atatacccga cagtattagc catagttgca
     3661 ggaactgttg ttatgttatt tatggctaag gttcaaaagc catgggcact atttatattt
     3721 ggtatgatat caccacttgt gatgtttgca gctggtcata cctacgtagt tgtggtttta
     3781 tcacttatag taatgataat agcagaatta attagaaaga ttggtaatta taattcattt
     3841 aaatacaata tgctttctta tgcaatcttc agcacatgga tatgtagctc tttaatgcaa
     3901 atgcttttag caaaagaaaa atatatggag tggtctttga tgacgatggg aaaagattat
     3961 gttgatgtat tagaaaagtt aataacttat cctcacatgg ctttagtagc cttaggtgct
     4021 ttcttaggag gaattcttgg agcatatata ggcaaggctc tattgaaaaa acactttgaa
     4081 aaagcaggca tcgtataatg cctacttttt cttcaaatgc tccctttaat cccaatccga
     4141 tctctaagat attagtcgta tttatcacag gcttaactgt tatgcatagc ataaacatcc
     4201 gttttgagct ggcaattgtt tgtcttattg gtattttgct ttatttgaat ggatacaaaa
     4261 aaacactttt caaatggatt atattgtgtg ggatacttta ctccttgcct aattttatgg
     4321 tgttatctga attaaaccct atagttaaga tgtttttgag tatacctatt gttattagaa
     4381 tgtttatttt accatttatg gcagcaagct ttatgataaa gacctcggat gtaggcgcaa
     4441 taatttcatc gatggataag cttaagattt caaagaatgt atccatacct attgcggtta
     4501 tgtttagatt cttcccatct tttaaggagg agaagaaaaa catcaaaata gctatgagag
     4561 taagagggat aaattttaaa aacccagtca aatatcttga atatgtttct gtgccactac
     4621 tcattatatc atctaatata tcagatgaca ttgcaaaagc ggcagaaaca aaggcaatag
     4681 aaaatccaat tgccaagacc agatacattc gcgtaaagat acagctaatt gattttgttt
     4741 atgttttagc ggttgctgga cttattgtgg gaggcttaat atggttgaaa taaaaaattt
     4801 aagtcttgat tatggtgaag agcatatatt agatgatata tcactatcca tagccgaggg
     4861 agagtgcgtg ctatttacag gaaaaagtgg aagtggtaag tcatctttaa taaattcaat
     4921 caatggacta gctgtaaggt atgataacgc aaagacaaag ggcgaaataa ttattgatgg
     4981 taagaatata aaaaatttgg aactttatca aatctcaatg cttgtttcaa ctgtttttca
     5041 aaatcctaag acatattttt ttaatgtcaa tacgacatta gaattattat tttatttgga
     5101 aaatatcggt cttgcaagag aagagatgga caggcgtttg aaggatatgc ttgagatatt
     5161 cccgataaaa aatcttttga acagaaatat atttaatcta tccggcggtg aaaaacaaat
     5221 tctttgcatt gcagcttctt atatagcagg tacaaagatt atagttatgg atgagtcttc
     5281 atcgaattta gatattaaaa gcataagtgt tttggcaaag atgctaaaga tattaaaaga
     5341 gaaaggcata agcataattg ttgcagagca tagaatttat tatttgatgg acatagttga
     5401 ccgt
//
LOCUS       DAVKQB010000086         5362 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKQB010000086 DAVKQB010000000
VERSION     DAVKQB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.086"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            125..1155
                     /locus_tag="VW528_002039"
                     /pseudo
     CDS             125..1155
                     /locus_tag="VW528_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1152..1370
                     /locus_tag="VW528_002040"
     CDS             1152..1370
                     /locus_tag="VW528_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912795.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETKPTDESGT"
     gene            complement(1427..1711)
                     /locus_tag="VW528_002041"
     CDS             complement(1427..1711)
                     /locus_tag="VW528_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9912796.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(1765..2700)
                     /locus_tag="VW528_002042"
     CDS             complement(1765..2700)
                     /locus_tag="VW528_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9912797.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            2804..3247
                     /locus_tag="VW528_002043"
     CDS             2804..3247
                     /locus_tag="VW528_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9912798.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSIGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVV"
     gene            3363..3629
                     /locus_tag="VW528_002044"
     CDS             3363..3629
                     /locus_tag="VW528_002044"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9912799.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            3626..4000
                     /gene="crcB"
                     /locus_tag="VW528_002045"
     CDS             3626..4000
                     /gene="crcB"
                     /locus_tag="VW528_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9912800.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            3994..4323
                     /gene="crcB"
                     /locus_tag="VW528_002046"
     CDS             3994..4323
                     /gene="crcB"
                     /locus_tag="VW528_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9912801.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            4442..4789
                     /gene="rplS"
                     /locus_tag="VW528_002047"
     CDS             4442..4789
                     /gene="rplS"
                     /locus_tag="VW528_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9912802.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            4831..4902
                     /locus_tag="VW528_002048"
     tRNA            4831..4902
                     /locus_tag="VW528_002048"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4864..4866,aa:Arg,seq:cct)
BASE COUNT         1618 a          948 c         1140 g         1656 t
ORIGIN      
        1 caattcagaa atcacttgtt aatttatccg tgatatgtta gatttgctag taaaattatg
       61 accattttgg aggaaagcaa tatttatacc agactaaaat caaaaagcat tatataatag
      121 tgatatgaaa tcaactaaag aagaaatcca agccatcaaa acacttttaa aagactctcg
      181 tacagctaaa tatcataaac gccttcaaat cgttctattt cgtctgatgg gcaaatctta
      241 taaagagatt atagagcttt tagactgtaa tcaaacaacg atttggcgaa atgtaaaaaa
      301 atatgaggag ttcggactcg actctctcct tcaagaaaca cgtggtggtc gtaaccatgc
      361 atatatgaca gttgaggaag agaaagcctt tcttgcccgc catttgaagg ctacagaggc
      421 aggagaattt gttacaatac cttatttcag gcttatatcg ttcctacaca cgtgatgcct
      481 tctatcaact gttgaagcgc catggttggc gaaatattat gccacgtcca gaacatccta
      541 agaaagcaga cgctcaaacc attgtcgcgt ctaaaaataa aatctcaatt caagaagaaa
      601 agaaagcgct ttaaaaccag tagacctttt cataaggttc gctcgatgta ccaagatgag
      661 gctggtttcg gtagaatcag taaactggga tcttgttggt ctccaatagg agtaggtcca
      721 catgtccata gtcactatat acgagaattt cgctattgtt atggagctgt tgatgcccat
      781 acaggcgaat catttttctt aagagctggt ggatgtaata ctgagtggat gaacgccttt
      841 ttagaagagc tttcacaagc ttatccagat gattatcttt tactcgttat ggacaatgct
      901 atatggcata aatcaagtac cttaaagatt ccgactaata ttggctttgc atttattcct
      961 ccgtacacac cagagatgaa ccccattgaa caagtgtgga aagagattcg taaacgtgga
     1021 tttaaaaata aagcctttca aactttggaa gatgtcatga atcaacttta agatatcata
     1081 caaggactgg agaaggaggt gataaagccc atcgttaatc ggagatggac tagaatgctt
     1141 tgtgaaagca gatgagtata agtcctcgtt ttgaaacctt agagcaggcc attgcctcca
     1201 aagatttgga aaaagtccga gaagctttta agaaaatgaa ttccacttgg accatcaatg
     1261 aaagtgtggt tcgtgacaat agtacagctc attatggacg tgttgaaaca gctatttcct
     1321 tcctgcctag tagcatggaa accaaaccta cagatgagtc tggaacatga aaacagcagt
     1381 tgatttcaag aaacaaaaaa gaacagccga aactgttctc ttatcattat ttgagaccgt
     1441 attttttgtt gaaacgatcc acgcgtccat ctgcttgagt gaacttttga cgtccagtgt
     1501 agaatgggtg tgagtctgat gaaatttcca cacggatcaa tgggtaagtt tcgccttcga
     1561 actcaactgt ttcgttagag cgttttgttg aaccgctaag gaattggtaa ccagtagttg
     1621 tgtccatgaa gacaactggg cgatattctg gatggatatc ttttttcatt ttgcaatatt
     1681 tcctttctgc catggtctct ttgcgagcca tagattgtta ccatactagt ctatcagatt
     1741 ctgaaaagtt tggcaagtat tttatcagtt tttaagcaag ttttttaact tttggtagat
     1801 gatttcgttt tcttctaggc tataggaatt agcaccactt gctagagggt ggcctccacc
     1861 atcatgctcc ttggcgattt cattgatagg atggatttta ctgcgtaagc gaactcggta
     1921 gtggccatca gcctgttcga caaaaattcc ccagagactc actctgtcaa tgcgtccagg
     1981 tgcaccgaca atggctgcag tttcagcatc ggttacattg tattgtttca agacttcttg
     2041 actcaggata acgcgagcag caccattttc atccacttcc agatggtcgt agatgtagcc
     2101 ttgcagttta gcaattttgt agctcatagt gtccattttg cgagtgagag ccgcaaagtc
     2161 aaagttatgt tctctcaaat aagcagccag gcgaagagtc cgtgcagtgg tagaagggta
     2221 gaggaagcga cctgtatcac cgacaattcc tgcaaagagc aactcagcat cgcgatctgc
     2281 caaggctagt tgggttgttt gggcaaatag ggtaatcatc tcgctagcgc tacttgaact
     2341 agtatcgacc caagacaggt caccgtatac atcatcattt ggatggtggt caatcttaat
     2401 gagaaaatca ccttgactat agcgcttatc atcgatacga gcagtattag ctgtatcaca
     2461 gacgatgaca agtgcgcctt ggtaggctct atcttcaaca agatccatct cagccatcca
     2521 agtaagagtt ggttcatcaa aaccgacggc tttgatggtt ttttctggga aatgatgttc
     2581 cagcaaggct ttcaatccca cctgacttcc caaggcatca gggtctggtt tcatatgacg
     2641 atgaatgata atggtgtcgt attctttaat tttttctaaa atttgttggc aaatctccat
     2701 aaataacctc aattctttct catttcattg tagcacaaga atttttattt ttaaaatgaa
     2761 aaagatgtga tataatggag aaagataaat tgaggaggac gatatggcat tagcaaaaat
     2821 tgtatttgcc agtatgaccg gtaataccga agaaattgca gatattgtag cagacaaatt
     2881 acgtgacttg ggcttggatg ttgatgttga tgaatgtaca actgttgacg cttcagactt
     2941 cttggaagca gacatcgcta tcgttgcgac ctatacttat ggtgatggtg aattgccaga
     3001 tgagatgatg gacttctacg aagacctagc agatctcaac ttgaatggca aaatctacgg
     3061 agtggtcggc tcaggagata ccttctacga tgaattctgt aaggctgttg atgactttga
     3121 ccgtgtcttt gtgtcaatag gagcagaaaa aggttcagag tgtgttaaag ttgatctttc
     3181 tgccgaggaa gaagatattg aacgcttgga acaattcgca gaagaattgg ttgctaaagt
     3241 agtataagca taaaagtgcg ctgatgtaat caatcagtgc atttttctta tcgagagttg
     3301 ggattttact agcctaattg atggcttttt gatacaataa ttcaaataaa ggaggagact
     3361 gtatggattt agatattatt cgtcaagaaa tcgatcaaat cgatgaccaa attgtcaaac
     3421 tgttagaaga acgaatgcat ttagttgagg gggtggttgc ttataagaaa gcttcaggta
     3481 aaccgatttt agatactaag agagaagaag ttatttttga aaaggttagg agtcgtgtag
     3541 aggacaagcg ctatcaggag actattgtag cgactttttc ggacatactc aaacgttcgc
     3601 gtgattatca ggatcaaaat atcaaatgaa aaaagaacaa ttttatccgc taggaatttt
     3661 tctagctgct atgttgggcg gacttgtccg atatctagtt tccacctggt taccagccag
     3721 tccagacttt ccttggggca ctctctttgt caactatctg ggaattttct gcttgatttt
     3781 tcttgtcaag ggctatctgg tctataaggg gactagtaag ggcttgattt tagcactggg
     3841 gacgggtttt tgcggaggtt taacaacttt ttccagccta atgcttgata ctgtgaagct
     3901 gcttgataca gggcgttatc ttagtttagt cctgtatttg cttttgagca tcggtggagg
     3961 cctactctta gcttattttc tagggaggaa gaaatggtaa tcgtctatct tgcaatcgcc
     4021 tgtggcttgg gagcactcgt acggtatttc ttttcccgct acaatcaagc ttctaaattg
     4081 ccactcggaa cgctcatagc taatctttta ggttgttttt taattggagt attctacaat
     4141 catgtagagt ctaaggaagt atatgctatt ctagcaacag gattttgtgg cggtttaaca
     4201 actttttcga ccttgaatga cgaacttcaa agactgttaa gtgataagaa ggtcttttat
     4261 tcttacctga ctttgactta cataggtggt ttggttgcga tttttttagg aattttgcta
     4321 taaatttctt tacttttttg tgaaaatttg gtagactgta tcttgtgtgt gatggacgca
     4381 caaaaataca gtttatccgc tgaggaaatt tcctcaagat tgacaaagat aggagaataa
     4441 aatgaatcca ttaatccaaa gcttgactga aggtcaactt cgtacagata tcccatcatt
     4501 ccgtcctggt gacactgttc gtgtacacgc gaaagttgtc gaaggtaacc gtgaacgtat
     4561 ccagattttt gaaggtgttg ttatcgcacg taaaggtgct ggaatctcag aaaactacac
     4621 agttcgtaaa atctctaacg gtgtaggtgt tgagcgtatc ttcccaatcc acactccacg
     4681 tgttgaaaaa atcgaagttg ttcgttacgg taaagtacgt cgtgcgaaat tgtactactt
     4741 gcgtgctctt caaggtaaag cagctcgtat caaagaaatc cgtcgttaat tcgactaaaa
     4801 aactctgctt ttcagcagag tttttatcta gctcccttag ttcaatggat ataacaactc
     4861 cctcctaagg agtagttgca ggttcgattc ctgcaggggg catttttagt aatgtaaaga
     4921 aagacaaaga aaattcaaaa tgtttatttg aaggcaattt tctcaagttc atttttaaaa
     4981 ataatttagc atttttaatt tttttgacca aattttgacc aaaaattata ttttggtcaa
     5041 tgattataga ataaaatgga tcataaaaat gtctttgatt tatagtagtg ctaaaaagga
     5101 cttcctttat tggaatgtcc ttatttttat tatggaattg ccaaatgacg ttaaggttca
     5161 gttctatgaa cttagaaaga aagtacaaag ctttaatcag ctttcaaaaa gatttggtat
     5221 ggatgtttct ggctaaggta tgtgacaagg tgaattgatc gttattgaac agagagcgtt
     5281 aaaaaaggga agaatcgtta ctattccact gaattaaaac aagaaataaa tcaacaaaag
     5341 cctatataat aacaaatgta tt
//
LOCUS       DAVKQB010000087         4285 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKQB010000087 DAVKQB010000000
VERSION     DAVKQB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.087"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..218
                     /locus_tag="VW528_002049"
     CDS             <1..218
                     /locus_tag="VW528_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912803.1"
                     /translation="GGKRLNTSIRSAYSGSDITPVYSLGSGSRIVMYYNGGGDNYIGS
                     GTRLAMAPQFGNHVRIHTSGSWSPDSY"
     gene            277..951
                     /locus_tag="VW528_002050"
     CDS             277..951
                     /locus_tag="VW528_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HEU9912804.1"
                     /translation="MLHPIFIIRRSWDGIFHLSEWKRNEEFDFFNKMDVPYLSMSSRI
                     EVTQAINFHKKHSISLYAIISWCVMSAINSIPELLMDTDGKIVWQYNQRGCSFTTLTS
                     EDKLNFSSFTMGDNLIEFVSAFNINKQKAEEGQKPNIDKNNIAYLSCVPWIDFLHVST
                     PMNLSKIDTVPRITWGKVIQENQRYFCTVNLQINHGMGDGLHVSNFFVLLQRFVNKIN
                     EYFQKK"
     gene            1019..2656
                     /locus_tag="VW528_002051"
     CDS             1019..2656
                     /locus_tag="VW528_002051"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9912805.1"
                     /translation="MSIFIGGAWPYANGSLHIGHAAALLPGDILARYYRQKGEEVLYV
                     SGSDCNGTPISIRAKKENKSVKEIADFYHKEFKETFEKLGFTYDLYSRTDSPLHHEIV
                     QELFLQLYEKKFLYTKKIKQLYCTFDNQFLPDRFVEGKCPNCGTHSRGDQCDNCSAIL
                     DPIDLVDKRCSICSNEPEVRETEHFYYVFSEFQNLLETYLNDAEETVRWRKNAINLTK
                     RYLREGLPDRAVTRDLPNGIPVPIDGFRDKKIYVWFEAVAGYYTASVDWAQKLQNNIT
                     DFWNNRTKSYYVHGKDNIPFHTIIWPAILSGLEIEPLPEYIISSEYLTLENKKISTSN
                     NWAIWLNDIIKKYDADSIRYFLTINAPEMKDANFSWREFIYSHNSELLGSYGNFINRT
                     LKFIEKYFESEIPTKYLEGEILYNLKELYTTVGNLVESGHMKQALEEIFEYIRSANKF
                     YDDMKPWALRESDIEKCKEVLATCVIIILNLGQMLNPFIPFSGKKIEDMFKTKLNTWN
                     YISNLPNKLSDVSMLFDRIDLKKIDEEVLELQQTSSR"
     gene            2757..3524
                     /locus_tag="VW528_002052"
     CDS             2757..3524
                     /locus_tag="VW528_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEU9912806.1"
                     /translation="MNNLTLLKEYNFRDLGNHLTQTGQKIKPKTLFRSSKLFGISKID
                     VDLLQSYGITKVIDFRSANEIKKAPDPDIKNIKNIVIPIFYNDDSELTEFPIEFFNKS
                     DAGFQHMIKTYDQMINQKQSKLGYKKFFKLLLSHPKDESLLFHCSMGKDRTGIASLFL
                     LYILGVDMNDIFHDYLLSNKYLINVRKENIEYVNNHSGNVILMHNLLSLSSAKEEYIN
                     RVLNVLDKEYGGILRYINTELGISSQEIEELKDRYLF"
BASE COUNT         1570 a          554 c          769 g         1392 t
ORIGIN      
        1 gtggcggtaa aaggttaaat acctctattc gtagcgccta tagtgggtca gatattaccc
       61 cggtatattc attggggtct ggctctagga ttgtcatgta ctataatgga ggtggtgaca
      121 attatattgg ttctggtact agattagcta tggcgccaca atttggaaat catgtaagaa
      181 ttcatacttc aggttcttgg agtccagatt cttattaact tacttgtcag agtaagcctt
      241 aaagatggtt gattgtgggt gtagcatgaa aaaagaatgc tacaccctat ttttattata
      301 aggaggagtt gggatggaat ttttcattta tcagagtgga aaaggaatga agaatttgac
      361 tttttcaata aaatggatgt tccttattta tctatgagtt cacgaataga agtaacgcag
      421 gctatcaatt ttcataaaaa acattcgata tcactttatg ccataatatc gtggtgtgtt
      481 atgagtgcaa taaattctat tccagaatta ctaatggata ccgacggaaa gattgtctgg
      541 caatacaatc aaagaggttg ttctttcact acccttacat cggaagataa gcttaatttt
      601 tcttcattta cgatgggaga taatttaatt gaatttgttt ctgcgttcaa cataaataaa
      661 cagaaggcag aagaagggca aaaaccaaat attgacaaga ataacattgc ttatctttct
      721 tgtgttccat ggattgattt tttacatgta agcactccta tgaatctttc aaaaattgat
      781 acagttcctc gaataacgtg gggaaaagtt attcaagaga accaaagata tttttgtaca
      841 gtgaatttac aaattaatca tggaatggga gatggattac acgtatctaa tttttttgtg
      901 ttattgcaaa ggtttgtaaa taaaataaat gaatattttc aaaaaaagta atgattgaaa
      961 agatattgca tttaaataat gttataataa aaataaagaa tttaaggaga aatacaatat
     1021 gtcaattttt attggaggag catggccata tgcaaacggt tcgttacata ttggtcacgc
     1081 ggcagcgctt ttaccggggg atattcttgc aagatactat cgtcagaagg gagaggaagt
     1141 tttatatgtt tctggaagtg attgtaatgg aacccctatt tctatcagag ctaaaaaaga
     1201 aaataagtct gtgaaagaaa ttgctgattt ttatcataag gaatttaaag aaacttttga
     1261 aaaattaggt tttacatatg acttgtactc gcgaacagat tctccactac atcatgagat
     1321 agtacaagaa ttatttttgc aattatacga gaagaaattt ttgtatacaa aaaaaattaa
     1381 acaactctat tgtacatttg ataatcaatt cttacctgat aggttcgttg aaggaaaatg
     1441 cccgaattgt ggaacccatt caagaggaga tcaatgtgat aattgctcag caattttaga
     1501 tcctatagat ttagttgata aaagatgttc tatttgttcc aatgaaccag aagttcgtga
     1561 gacagaacac ttttattatg tttttagtga gtttcaaaat ttgttagaga cttacttaaa
     1621 tgatgctgaa gaaacagtga gatggcgaaa aaatgcaata aatctaacta aaaggtattt
     1681 acgggaaggt ttacctgatc gagctgttac tcgtgatcta ccaaatggga ttccagttcc
     1741 cattgatggt tttagagata aaaaaatata tgtctggttt gaagcggtag caggttacta
     1801 cacagcaagt gttgattggg cgcaaaaact tcaaaataat attactgatt tttggaacaa
     1861 tcggacaaag tcatattatg ttcatggaaa agataacatc ccatttcata ctattatttg
     1921 gccggcaata ctctcaggtt tagaaataga gcctcttcca gagtatatta tttctagtga
     1981 atatttaacg ttagaaaaca aaaaaatatc aacaagtaat aattgggcta tatggcttaa
     2041 cgatatcata aaaaaatatg atgctgattc aatccgatac ttcctaacaa taaatgctcc
     2101 tgagatgaaa gatgcaaatt tttcttggag agaatttatt tatagtcata actcagaatt
     2161 attaggatcg tacggtaatt ttatcaatag gactctaaag tttattgaaa agtattttga
     2221 atctgaaata cccactaaat atctcgaagg agaaatttta tataatttga aagaattata
     2281 tacaacagta ggtaatttag ttgagagcgg gcatatgaaa caggcattag aagaaatttt
     2341 tgaatatatt agatcggcta ataaattcta tgatgatatg aaaccgtggg cattgcgaga
     2401 gtccgatatt gaaaaatgta aagaagtctt agctacttgt gtaatcatta tacttaattt
     2461 aggacaaatg ttaaatccat tcattccttt ttctggtaaa aagattgagg atatgtttaa
     2521 aacaaagcta aatacttgga attatatttc aaaccttcct aacaagctat cagatgtatc
     2581 aatgttgttt gataggattg atttgaaaaa aattgacgag gaagttttag aattgcaaca
     2641 aacatccagt agataattca aaaatactcg aaagtagcct tttttaattt aaattaacaa
     2701 ccggagatgt tgaataaaat aaagatggtt ttttgagcat attatggaga aaagcattga
     2761 ataatctaac tttactaaaa gaatataatt ttagagatct tggaaaccac ctcactcaaa
     2821 caggacaaaa aataaaacct aaaacattat tccgttcaag taaactattt ggaatctcta
     2881 aaattgatgt cgatttgtta cagagctatg gaataacaaa agtcattgac tttcgatcag
     2941 cgaatgaaat aaaaaaagct ccagatccag atataaaaaa tataaaaaat atagtaattc
     3001 caatattcta taatgatgat tcagagttga cagaattccc aatagaattt tttaacaaat
     3061 cagatgcagg ttttcaacat atgataaaaa cttatgatca aatgatcaat caaaaacaga
     3121 gtaaacttgg ttacaagaaa ttctttaaat tattactaag tcatccaaaa gatgaatctc
     3181 ttttgtttca ttgttcaatg gggaaggatc gaacaggaat agctagcttg ttcttgcttt
     3241 atatattggg agtagatatg aacgatattt ttcatgatta cttactatct aacaagtatt
     3301 taataaatgt gagaaaagaa aatattgaat atgtaaataa ccatagtggg aatgtaatat
     3361 tgatgcataa tttactttca ctcagttcag caaaagaaga atatattaat cgagtattga
     3421 atgtccttga taaagaatat ggaggaatac ttagatatat taatacagaa ctaggaataa
     3481 gtagtcaaga aattgaggaa ttgaaagata gatatctttt ttagaatagt aagtactaaa
     3541 aatgaatata taacagtgcg aacggcggtt ttgtttttgt ctaattctac taatttaaca
     3601 taagcaaaaa cggattgttt tctatgtaag tccttaacta aattgatgga aagcacgtta
     3661 aattatgtgc aaaatgcgta caaaatagtg tttatcaaat gtaaaatttc ctacttgtca
     3721 tcaaagttca acaaatgtag gttgtttggt tatttgggta gatttctgga cttatcgttg
     3781 gttgtagaga cataatgttg cagttaaagc ttagagaata atctccgtat ttttgttagg
     3841 caatttaaaa gttaagacaa agtgagtttg tctcttaatt caactcacca caaacacatc
     3901 acctttttat tataacttcc gaccaatttt tttgaagaga aaaatgaaaa aacaccaact
     3961 gtgcagggta gctgacaagg cttctagctt ccatttattg aaaccaaatc aatctgttat
     4021 gagataatag cagttgcaga agctgactac tttgagaagt taaatgaggt ttattatcaa
     4081 gaatacttca tgaatatagt tataagggag ggggtagttg aaagaattga ctcatctgtt
     4141 aaaataaaaa tggaaataat aacatcataa tagtgtgact tagtattttt cagcatcttg
     4201 gaagtaggta aatgatatta aaattatact gcatagtaaa ggatagtaat gaggaattta
     4261 atatcttcct gtcaatatct tcagt
//
LOCUS       DAVKQB010000088         4011 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKQB010000088 DAVKQB010000000
VERSION     DAVKQB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.088"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(146..1207)
                     /gene="dinB"
                     /locus_tag="VW528_002053"
     CDS             complement(146..1207)
                     /gene="dinB"
                     /locus_tag="VW528_002053"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9912807.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            1556..3880
                     /gene="pflB"
                     /locus_tag="VW528_002054"
     CDS             1556..3880
                     /gene="pflB"
                     /locus_tag="VW528_002054"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9912808.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1207 a          846 c          777 g         1181 t
ORIGIN      
        1 aaaaaccttg aaggggcaac tcttcaaggt tttatactct tagaaaatct cttcaaaccg
       61 cgtcagcttt atctgcaacc tcaaaacagt gttttgagca gcctgcggct agcttcctag
      121 tttgctcttt gattttcatt gagctttaaa atccagtcag ggtaatcccc aataggcgga
      181 cacctctttc tttctcgctt aattcttcat agagttgcag ggctatttgg cttatctgac
      241 tagcatcttg tgttttttga gcaagacttt ttcgtttggt aagagttgaa aagtcctcgt
      301 agcggatttt caaaatgaca atttttccag ctttttcttg ttgatgtaga ttgagagcga
      361 ctttttctga tagaagagtc agctcttttt tgatatcttc ctcagcaagg agaatcttcc
      421 cgtaggtttt ctccttgccg attgatttac ggatgcgatt ggatttgact ggagagttgt
      481 gaatgccacg agcctttcga tacagatcat agcctagtct accaaaacgg tctattaggg
      541 ttacctcagg aacttcaagt aaatcagcac cagtaaaaac gcccatttga tgaagacgtt
      601 ctactgtctt ttttcctact ccatgaaatt tggaaatatc catttgtttg agaaaatcct
      661 cagcctgttc aggtagaatc actgtcaaac catgtggttt ttgataatca ctcgccattt
      721 tagctaagaa tttgttgtaa gaaacgcctg cggaagcagt tagatggagt tcttgccaga
      781 tatctttttg aatgaggcga gcaattttga ccgctgactt gataccgagt ttattttctg
      841 tcacatccaa ataggcttcg tcaatgctca tgggttcaat caaatctgta tagcgcttaa
      901 aaatagctcg aatctggagt cccacagact tgtatttctc ataattccct gagataaaga
      961 cagcctgggg acaacgttca taagcttcct tggaactcat ggcagaatgg acaccaaaag
     1021 ctcttgcctc ataactacag gtagaaacga ctccccgtcc acctgtttgc cgagggtcgc
     1081 ttccaataat gacaggtttt cctctgagtt taggattatc cctgatttcc actgcagcaa
     1141 aaaaggcatc catgtcaata tggatgattt ttcttgacaa atcatttaac aaaggaaaaa
     1201 tcaacatgcc tagcaccttt ttatactctt cgaaaatctc ttcaaaccac gtcagctcta
     1261 tctgcaacct caaaacagtg ttttgagcaa tctgcggcta gcttcctagt ttgcttttcg
     1321 atttccattg agtgttactg cttattttct tttattatac ccttttttct gaaaaaaaga
     1381 aaaaaggact ttattttttc aaaaatataa tacagtttga aataaaatat agactgtttt
     1441 agaaaagaaa gtgtaaaaat agggaatttt cacttgttga aatcggttac tatatggtat
     1501 acttgtctta tgaatgtaac agatgactgt tactagaaaa aaagaggaca ttaatatggt
     1561 tgttaagaca gttgttgaag cacaagatat ttttgacaaa gcttgggaag gcttcaaagg
     1621 cgtagattgg aaagaaaaag caagtgtatc acgatttgta caagctaact acacacctta
     1681 tgatggagac gaaagcttcc ttgcaggacc aacagagcgt tcacttcaca tcaagaaaat
     1741 tgtagaagaa actaaagcac actacgaaga aactcgtttc ccaatggaca ctcgtccaac
     1801 atctatcgct gatatccctg ctggatttat cgacaaagaa aatgaagtta tctttggtat
     1861 ccaaaatgat gaactcttca aattgaactt catgccaaaa ggtggtatcc gtatggctga
     1921 aactacttta aaagaaaatg gatacgaacc agacccagct gttcacgaaa tcttcactaa
     1981 atatgtaaca acagttaacg acggtatttt ccgtgcctac acttcaaata ttcgtcgcgc
     2041 tcgtcatgca cacactgtaa ctggtcttcc agatgcatac tcacgcggac gtatcatcgg
     2101 tgtttacgca cgtcttgctc tttacggtgc agactacttg atgcaagaaa aagtaaacga
     2161 ctggaatgca atcaaagaaa tcgatgaaga aacaatccgt cttcgtgaag aagtaaacct
     2221 tcaataccaa gcattgcaac aagttgttcg cctgggtgac ctttacgggg ttgatgttcg
     2281 caaaccagcg atgaacgtga aagaagcaat ccaatgggtt aacattgctt tcatggctgt
     2341 ctgccgtgtg attaacggtg ctgctacatc tctaggtcgt gtaccaatcg tattggacat
     2401 ctttgcagaa cgtgaccttg ctcgtggtac atttactgaa tcagaaatcc aagaattcgt
     2461 tgatgatttc gttatgaaac ttcgtacagt taaatttgct cgtacaaaag cttatgacca
     2521 attgtactca ggtgacccaa cctttatcac aacttctatg gctggtatgg gtaacgacgg
     2581 tcgtcaccgt gttactaaga tggactaccg tttcttgaac acacttgaca acatcggtaa
     2641 ctcaccagaa ccaaacttga cagttctttg gactgacaaa ttgccataca acttccgtcg
     2701 ctactgtatg cacatgagcc acaaacactc ttctatccaa tacgaaggtg taacaacaat
     2761 ggctaaagac ggatatggtg aaatgagctg tatctcatgc tgtgtgtctc cacttgatcc
     2821 agaaaatgaa gaacaacgcc acaacatcca gtacttcggt gctcgtgtaa acgttcttaa
     2881 agcccttctt actggtttga atggtggtta cgacgatgtt cacaaagact acaaagtatt
     2941 tgatatcgaa ccaatccgtg acgaagttct tgaattcgaa tcagttaaag cgaactttga
     3001 aaaatctctt gactggttga ctgacactta cgtagatgcc ttgaacatca tccactacat
     3061 gactgataga tacaactacg aagctgttca aatggccttc ttgccaacta aacaacgtgc
     3121 caacatggga ttcggtatct gtggatttgc taacactgtt gatacattgt cagctatcaa
     3181 atacgctaca gttaaaccaa tccgtgacga agatggctac atctacgatt acgaaacaat
     3241 cggtgactac ccacgctggg gtgaagatga cccacgttca aacgaattgg cagaatggtt
     3301 gatcgaagct tacacaactc gtctacgtag ccacaaacta tacaaagacg cagaagctac
     3361 agtatcactt ttgacaatca catctaacgt tgcttactct aaacaaactg gtaactcacc
     3421 agttcacaaa ggtgtatacc tcaacgaaga tggttctgtg aacttgtcta aacttgaatt
     3481 cttctcacca ggtgctaacc catctaacaa agctaaaggt ggttggttgc aaaacttgaa
     3541 ctcactttct agccttgact ttagttatgc agctgacggt atctcattga ctacacaagt
     3601 atcacctcgc gctcttggta agactcgtga tgaacaagtt gataacttgg taacaatcct
     3661 tgatggttac ttcgaaaacg gtggacaaca cgttaacttg aacgttatgg acttgaacga
     3721 tgtttacgaa aaaatcatgt caggcgaaga cgttatcgta cgtatctctg gatactgtgt
     3781 aaacactaaa tacctcactc cagaacaaaa aactgaattg acacaacgtg tcttccacga
     3841 agttctttca atggatgacg ccttggatgc attgagctaa tcaagttcgg ctctttgtca
     3901 actgcagtgg gttgaagtcg gctaagctcg agaaaggaca aaattcgtcc tttctttttt
     3961 gatgttcaga gcgataaaaa tccttttttt gaagttttca aagttccgaa a
//
LOCUS       DAVKQB010000089         4008 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKQB010000089 DAVKQB010000000
VERSION     DAVKQB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.089"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(173..1588)
                     /locus_tag="VW528_002055"
     CDS             complement(173..1588)
                     /locus_tag="VW528_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9912809.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAKLLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1601..3439)
                     /locus_tag="VW528_002056"
     CDS             complement(1601..3439)
                     /locus_tag="VW528_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9912810.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVIPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     DVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDESVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3457..>4008)
                     /locus_tag="VW528_002057"
     CDS             complement(3457..>4008)
                     /locus_tag="VW528_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9912811.1"
                     /translation="TDHISSSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIK
                     KELGIELPIDEAAFIALHFVNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSI
                     DYYRFITHIKLFAHRLVENTTYCDDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYN
                     YLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1342 a          803 c          526 g         1337 t
ORIGIN      
        1 ccaatctatc ctaagttaac tatttataat cggctctctg taacaaaatt aaattaatta
       61 atccccctat aaaataaaga ggtttagaat ttgatgtctt ccaaacctct ttatttcata
      121 ttcaatgaga tgttgcctta accgtgataa tatctaatca tcaataaaca aactatttga
      181 ataaggattc tccatttgat tcaatcactc ctttatacca agtaaaggac attttcttat
      241 atcgattgaa tgtgccattt ccatcatcat ttcgatcaac ataaatgaga ccgtacctct
      301 tagaaagctg tgcagtggac atagaaacgc agtcaataca tccccaagac gtatagccca
      361 taatttccac accatcttgt agagcttcag ctacttgcaa taaatgttct ttcatatact
      421 gaattctata atcatcttgg actgttaagt tattaaattc atcttttatt agttgatctt
      481 tagcacctaa tccgttttcc actataaata atggaatttg ataacggtca taatatctat
      541 ttaaaattat acgtagtcca attggatcaa tttgccatcc ccactcagaa gactctaaat
      601 aaggatttac taagccacca ataatattcc cttctcctga attatactgt gttggaagag
      661 cagattgagt cacactcatg taatagctaa aggataaaaa atctacggta taatttttta
      721 ataactttgc atcttcagct gcaaactcta tgttaatgtc attttcctta aaatatcttt
      781 ttgcataatt cggataataa cctctaacat gcacatctga aaatagataa tttagattct
      841 catactcatg agtcgcccat acatcttttg gatttggagt cattggataa gctggcatag
      901 ctaataccat acaccccacc ttaaactctg aattaatctc acgagcaatt tttgtaacca
      961 aactcgaggc gactaattca tgatgtatag cttgatataa ttcttgtttc gaaagattct
     1021 ccttaggtat atctattcct ccactagtaa acggtaattc caaaacagag tttacttcgt
     1081 taaatgtaag ccaatattta actttatctt tatacctttc taaaactgtt cgagcaaatt
     1141 tttcataaaa atgaatcatt ctcctatcaa tccatccatg atattttctt gctaaatata
     1201 atggagtctc atagtgtgaa agagttacaa gtggttctat cccgtgagca tgtagttcat
     1261 caaacaattc atcataatat ttcaacccag cttcgttagg ttcttcctca tctccttttg
     1321 gaaaaattct actccatgca atagaagtac gaaaaacatt aaagcccatt tcagaaaaca
     1381 aggatatatc ttccttatat ttatgataaa aatcaatacc tatcaatttt aagttatctt
     1441 ctgtaggatt ttctgttgct tctcctaatc cacctttggg taacacatcc tgaactgata
     1501 agcccttacc atcttcatta tatgctccct ctacttgatt agctgcaaca gctccacccc
     1561 aaagaaaatc atctggaaaa atggtcataa ctttcctcca ttataatatt accagtaatt
     1621 ccttagaatg ctcgattgtc tgattattag gtaatactaa tacatctaga aaatcattgg
     1681 tattcgttac aattactggt gtaactgttt cgtagccttt agtcttgatt aaattcaagt
     1741 ccatttcaaa aatcaactga tttttgaaaa ctctgtctcc ttcttctaca tgactaataa
     1801 aaccttgacc ttttagctca acagtatcta atccaatatg aattagtaac tcaacactct
     1861 catcactctt caatccaatt gcgtgcttag tcggaaaaat atttgtaatt ttcccatcaa
     1921 atggtgcata aaccttacct tcacttggaa taatcgctac tccgtctcca attagtttat
     1981 ctgaaaatgt tttatcctgg acatcgctta acggaatgat ttctcctgat ataggagaaa
     2041 atatcatttt tttatttgaa actccagctt taacttctaa attgctagaa ctctcttctt
     2101 catcgattcc aaatatataa gctaatacaa aggtaataac aaccgaaata accgccacaa
     2161 ttaaagcatt tacaatattt gatggtacat cagaataaat aaattgaggc aacgctatca
     2221 aagatgggac agcaaataga tacgctttaa cactagtaag acctgcaaat aatcccgcta
     2281 atccaccacc aatcatagct gcataaagcg gttttttata ttttaaagtc acaccatata
     2341 atgcaggttc ggtaatccct gcaagtaagg ctgagaaacc tgctgcaaaa gcaatttgtt
     2401 ttgtattatt atttttactc tttaatgcaa cagccatcga agcagcccct tgagctaagt
     2461 ttgaccctaa cattgctgga agaattaata cgtctggagt agcaatagat gccgccaaaa
     2521 aaataggtgc aaaagcccaa tgcattccag tcataacaat aaatggcata atagcaccaa
     2581 gaatagctaa tgtaagccat ccagctacat catacatttg cccaactaga tttgataatc
     2641 cttcaccaac aattactcca ataggcccga ctacaactaa ggcaatacag cttgatacta
     2701 ataatactag cgtaggttgc aaaaaactct tagtaacagc tggtgttaat ttagcaatca
     2761 ttttttcaat atatttcatc aaccaaacca taataagaat tggaacgact gacgaaccat
     2821 aactagctgg tatcacaggt acaccaaata aactaagagg attccctgat tgcaccattt
     2881 gaacaaaatt tggatggaga agtacacctg ctacagacat agctaatgta gatgttactt
     2941 ttaatttttg tgatgcagaa taagctaata acagcggtaa gaaataatat ggagcatccc
     3001 caaaaaatgt caaaaaagca atagtctgag aatctgattg caatatacca agcattggta
     3061 aaataattac caagactttc agcatacctc cccctaacat tgctggaatg attggagtca
     3121 tggaaccagc gatatactca atgattcttt ctaaaatatt ccctttgtgc ccttgaacaa
     3181 ctgaatcgga ttcaaaattg ccaagtttaa cgaattcttt ataataatta gctacatcat
     3241 taccaagtat aatttgatat tgtccattct ttttcataat acctattaca cctggtatct
     3301 tcttcacatc atcatcattg actaaatttt catcttttaa ttctaatctt aaacgtgtta
     3361 cacaatgggt aactctattg acattttttt cacctccaat tacatcgagg attttttgta
     3421 ccttatcttt ataactcatg gtattctcct attctattaa tctaaatttt ttgttaagcg
     3481 acgaatatga gccatcaaat aaactaattc actagaagtc agcaaataat tgtactcagt
     3541 ttgtataaac attgccacct gttcaccaca ttcatattct ctaggatatt tatttttcat
     3601 taatgctaac aagtcttcat catcatcatc gtcacaataa gttgtatttt caaccaagcg
     3661 atgggcaaat aattttatat gagttatgaa tctataataa tcaatagaat cttggttaaa
     3721 ctcagtacaa tagaaatctt gaatgatttt ctcaattccc ataattattt cagtgatttt
     3781 atacgactct tgaaaattat tttctaaatt agcattaaca aaatgtagcg ctatgaatgc
     3841 agcttcgtca attggaagtt caatacctaa ctcttttttt attatttgta aagccctttt
     3901 cccaagctca aattcttctt tataatattt cgagatttcc cagcgtaggg gattcgaaat
     3961 aataactcct tctttataac gttctatact cgaagaaata tgatccgt
//
LOCUS       DAVKQB010000090         3944 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKQB010000090 DAVKQB010000000
VERSION     DAVKQB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.090"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(81..713)
                     /locus_tag="VW528_002058"
     CDS             complement(81..713)
                     /locus_tag="VW528_002058"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9912812.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKDDQENWQEG"
     gene            complement(747..1448)
                     /gene="pyrF"
                     /locus_tag="VW528_002059"
     CDS             complement(747..1448)
                     /gene="pyrF"
                     /locus_tag="VW528_002059"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9912813.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            1769..1861
                     /locus_tag="VW528_002060"
                     /pseudo
     CDS             1769..1861
                     /locus_tag="VW528_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2183..2896)
                     /locus_tag="VW528_002061"
     CDS             complement(2183..2896)
                     /locus_tag="VW528_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9912814.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(2889..3722)
                     /locus_tag="VW528_002062"
     CDS             complement(2889..3722)
                     /locus_tag="VW528_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912815.1"
                     /translation="MIEVSHLSKSFGDKIALNDISFTVKEGQIFGFLGPSGSGKTTTI
                     NILTGQLLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMSLYNNLLFYSKFY
                     NISKLRVDNLLKRVGLYDSCKMVAGKLSTGMRQRMLLARALINKPAVLFLDEPTSGLD
                     PTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKLVEQGAPSELIQR
                     YNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLTGGKLN
                     A"
BASE COUNT         1239 a          794 c          658 g         1253 t
ORIGIN      
        1 attttattta aaatattgtc atattttaaa taaaatggta aaagaggctc ggtagcctcg
       61 tctctcttta tcttactgac ctaaccttct tgccaatttt cttggtcgtc tttaaagcgt
      121 tttagaagat caaggccctc tggcgtgatg taaccttctt cttgggctag atggataagc
      181 tcgctatagt ttgaaagcgt cacaagttta acaccagcat ctgcaaagtt cttatctgct
      241 tttggcaatt ggtagctgaa aatcgctaca actccaagca catctgctcc ttctcgcttg
      301 gctgctgcta cagcttcaag aactgaacca cccgttgaaa taaggtcttc aaccactacc
      361 attttttgac cttgagctac gcgaccttcg atttgattac cagctccatg gtcttttggt
      421 ttgctacgga tgtaggcaaa aggcaagtcc atcttatcag caataatggc tccgtgtgga
      481 atccctgctg ttgcagttcc tgcaatcact tctacttcag gaaaggcttc tttgatagct
      541 tccacaaaac cattttcaat tagggtacga gtttctggat aggctagtgt cacacgatta
      601 tcagtgtaaa tcggtgactt gataccagat gcccaagtga agggttcctc tggtttgagg
      661 taaacggctt ggattttcaa gaggtggcta gcgatatctt tagcaagtgt catggtattc
      721 tccttttatt tttctaatct agttctttaa ttccagtcct gtgtccattc atccttgatg
      781 gcatgataag ctgcaacagg atcctcagct tgggtaatgg gacgtcccac tacgatatag
      841 tcactgccga tttgataggc atcagcaggt gtcatgactc gtttttgatc tccaactgca
      901 acaccagctg gacgaatccc tggtgtcaga cagataaaat ctggattggt agcctgcttg
      961 atgacttgta cttcctgagc cgagcaaaca acaccatcca agccagcttc agctgtcttc
     1021 ttggcatagt gaatcacaga ctcttgcaga ctggtttgga tattttgaaa ctcctgcatc
     1081 tgagcttctg acgttgatgt gagctgagtt acagcgatca atttggcttg acttccaaga
     1141 ccttcacgcg cagccttcat catctctaca ccaccagccg catgaacatt ggtcatatcc
     1201 acaccaagct gagataggat cttcattgct gacttgactg tattaggaat gtcatgaagt
     1261 ttgagatcca aaaagacact atgacccaaa ccttttaagt aggacacaat ctcaggcccc
     1321 gctgcgtaat aaagctccat ccctaccttg agataaaggc tttcttctgc tgggaaaaga
     1381 gctaaaaatt ccttgaccgc ctcaaaacta ggaaaatcaa gagcaatgat tggacgatgt
     1441 tctcgcatag gtttctcctt ttacctttac tagtgagaga gtctggccaa cagaagccac
     1501 gaaaaaagga acctgccaac agcaaggttc cacgaaaaat gggtagtctc caccctttca
     1561 ccgtctaacc ttagctgcct ctctggactg ctttaaaggt tttttactat tctattattt
     1621 ctactagcac ttgtcaagta aaaacatggt cacgtatgtg ttgactgcta ttgaacacct
     1681 cacaagactc tttccaaaag aatagttaaa gactttcact taggacagag aaaaagagtt
     1741 tgcttgttac cctttaattg aacaaatggg aattctgttt tattttgcgg atgcctattc
     1801 atcttggcaa aggggaagta atgagaatgc gaatggactt cttcgtgagt attttcttaa
     1861 accgatttgg caacaattac cgaaaaaatc cttaatggtg ctttgctagc ttattaatca
     1921 ccgtccccaa aaatacttgg attacaagac aggttttgaa gcacttatag aggagattta
     1981 aaaatgttgc attttttatt gcaatttatc agttatctta tcacccaaaa cttctgcaat
     2041 tagtatgagc actattgtta tttttctttt ttcgtacact caaatcaaaa tatagcaata
     2101 agactattct gtagaattgc tgtcaaaaag atcagttcat ttttccttcc cacttgtgtt
     2161 tataatcatt aaaataactc aattaagaaa ttttcttttt cctaatagtt atcgtaatta
     2221 aagttaatag aagaacaatc caaataagta gtgttagatt aggaattaga gtttcattcc
     2281 atgaaaatcc ctcccatttt gtgaaaaact tagtaaacag tcccataaaa ctataatctg
     2341 ttatcttcgc aactgtctta tccaaaccag atagcatcgg taagaaagca actaaaatca
     2401 tagcaggaag actgataacc tgagctacta cttggctctt cgcggttaaa cctatcaata
     2461 aatagaataa aatgatggat aaagaggtta gaagaacgat tgtaatataa ttaaaagtat
     2521 gtacaattgt aactcctaaa ataagaggag tagttcccat aatcacaaaa gttagcaaaa
     2581 aaggaagaaa catcgttgat aaaatgtatt cggatccttt aacaccactc aacagaagag
     2641 tttgtaaatt gtacttttct ttttcttcag acaagataat agttatagga cttccaacag
     2701 ccaaagccaa agaaaaaggt aaacacatca tcaaaagaac taatgcctgt tgatcgttga
     2761 ccttcccctg tgtttccata agatatttat aaaaatatgt gaaagcaaaa ggcactaaaa
     2821 cttgcaataa aatactctta ttggaaagga taatttgaca acgcaaccat accaaagcca
     2881 gaaaccgttt aagcatttag ctttcctcct gttaatgtga taaaaatatc ttctaaagta
     2941 ggctcacatg aatgaattga aaaaatattt tccagatcag cctgagatac ctgttctagt
     3001 gatgtaaaat caaaagttat ctgattccca ttataatctg taaccttaat ctttttatct
     3061 ttattatatc tttgaatgag ttcagaagga gctccttgct caactaattt ccctttattt
     3121 aataaggcaa cataatcaca taaaagagtt gcttcattca tatcatgagt cgttagaaaa
     3181 atcgttgtcc ctgctgtttt caattctaaa attaactcat gaattgttcg agaagttgtg
     3241 ggatctagac ctgaggtcgg ttcatccaga aagagtacag cgggtttgtt gataagagct
     3301 cgtgctaaaa gcattcgttg cctcattcca gtggataatt ttcctgctac catcttgcaa
     3361 ctatcatata atcctactcg ctttaacaaa ttatcaacac gtaatttact aatattataa
     3421 aatttactat aaaaaagaag attgttatac agagacattt tctcataaaa tccacttgta
     3481 tcgctaacca atccaattct ctttaattca ccgcttgtta aattttgaga tttttgtccc
     3541 aaaataatag attgtccttt atcggcaagg agctgcccag tcagaatatt aatcgttgtg
     3601 gtctttccag aaccagatgg tcctaaaaat ccaaaaatct gaccttcttt aacagtgaaa
     3661 cttatatcat ttaaagctat tttatcacca aaactttttg ataaatggct aacttcaatc
     3721 atactcattg cccctccttt atattctaca tttttactta acatctattt acttagcttt
     3781 atcaaataaa cgtcttaaaa aaaacaataa cattaagcat ataaccgtta gcaagattgt
     3841 ttcaatatta ttgataatat gatacattct caagcagtat aaaataatta tatatatgca
     3901 aatgctcatt atacctattt ttttaaacat actaagcctc cttg
//
LOCUS       DAVKQB010000091         3770 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKQB010000091 DAVKQB010000000
VERSION     DAVKQB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.091"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            160..387
                     /locus_tag="VW528_002063"
     CDS             160..387
                     /locus_tag="VW528_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912816.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            675..3572
                     /gene="nanA"
                     /locus_tag="VW528_002064"
     CDS             675..3572
                     /gene="nanA"
                     /locus_tag="VW528_002064"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU9912817.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKEIDGKTYQILY
                     REGEKEAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1294 a          624 c          850 g         1002 t
ORIGIN      
        1 gcgttttaaa aatgaggtat aataaattta ctaacactta taaaaagtga tagaatctat
       61 ctttatgtat atttaaagat agattgctgt aaaaatagta gtagctatgc gaaataacag
      121 atagagagaa gggattgaag cttagaaaag gggaataata tgatatttaa ggcattcaag
      181 acaaaaaagc agagaaaaag acaagttgaa ctacttttga cagttttttt cgacagtttt
      241 ctgattgatt tatttcttca cttatttggg attgtcccct ttaagctgga taagattctg
      301 attgtgagct tgattatatt tcccattatt tctacaagta tttatgctta tgaaaagcta
      361 tttgaaaaag tgttcgataa ggattgagca ggaagtatgg tataaatagc ataagctgat
      421 gtccatcatt tgcttataaa gagatatttt agtttaattg cagcggtgtc ctggtagata
      481 aactagattg gcaggagtct gattggagaa aggagagggg aaaattggca ccaatttgag
      541 atagtttgtt tagttcattt ttgtcattta aatgaactgt agtaaaagaa agttaataaa
      601 agacaaacta agtgcatttt ctggagtaaa tgtcttattt cagaaatcgg gatatagata
      661 tagagaggat cagtatgaat cggagtgttc aagaacgtaa gtgtcgttat agcattagga
      721 aactatcggt aggagcggtt tctatgattg taggagcagt ggtatttgga acgtctcctg
      781 ttttagctca agaaggggca agtgagcaac ctctggcaaa tgaaactcaa ctttcggggg
      841 agagctcaac cctaactgat acagaaaaga gccagccttc ttcagagact gaactttctg
      901 gcaataagca agaacaagaa aggaaagata agcaagaaga aaaaattcca agagattact
      961 atgcacgaga tttggaaaat gtcgaaacag tgatagaaaa agaagatgtt gaaaccaatg
     1021 cttcaaatgg tcagagagtt gatttatcaa gtgaactaga taaactaaag aaacttgaaa
     1081 acgcaacagt tcacatggag tttaagccag atgccaaggc cccagcattc tataatctct
     1141 tttctgtgtc aagtgctact aaaaaagatg agtacttcac tatggcagtt tacaataata
     1201 ctgctactct agaggggcgt ggttcggatg ggaaacagtt ttacaataat tacaacgatg
     1261 cacccttaaa agttaaacca ggtcagtgga attctgtgac tttcacagtt gaaaaaccga
     1321 cagcagaatt gccaaatggt cgagttcgcc tgtatgttaa tggtgtttta tctcgaacaa
     1381 gtctgaaatc tggcaatttc attaaagata tgccagatgt aacgcatgtg caaatcggag
     1441 caaccaagcg tgccaacaat acggtttggg ggtcaaatct acagattcgg aatctcactg
     1501 tgtataatcg tgctttaaca ccagaagagg tacaaaaacg tagtcaactt tttaaacgct
     1561 cagatttaga aaaaaaacta cctgaaggag cggctttaac agagaaaacg gacatattcg
     1621 aaagcgggcg taacggtaaa ccaaataaag atggaatcaa gagttatcgt attccagcac
     1681 ttctcaagac agataaagga actttgatcg caggtgcaga tgaacgccgt ctccattcga
     1741 gtgactgggg tgatatcggt atggtcatca gacgtagtga agataatggt aaaacttggg
     1801 gtgaccgagt aaccattacc aacttacgtg acaatccaaa agcttctgac ccatcgatcg
     1861 gttcaccagt gaatatcgat atggtgttgg ttcaagatcc tgaaaccaaa cgaatctttt
     1921 ctatctatga catgttccca gaagggaagg gaatctttgg aatgtcttca caaaaagaag
     1981 aagcctacaa agaaatcgat ggaaaaacct atcaaatcct ctatcgtgag ggagaaaagg
     2041 aagcttatac cattcgagaa aatggtactg tctatacacc agatggtaag gcgacagact
     2101 atcgcgttgt tgtagatcct gttaaaccag cctatagcga caagggtgat ctatacaagg
     2161 gtgaccaatt actaggaaat atctacttca caacaaacaa aacttctcca tttagaattg
     2221 ccaaggatag ctatctatgg atgtcctaca gtgatgacga cgggaagaca tggtcagcgc
     2281 ctcaagatat tactccgatg gtcaaagccg attggatgaa attcttgggt gtaggtcctg
     2341 gaacaggaat tgtacttcgg aatgggcctc acaagggacg gattttgata ccggtttata
     2401 cgactaataa tgtatctcac ttaaatggct cgcaatcttc tcgtgtcatc tattcagatg
     2461 atcatggaaa aacttggcat gctggagaag cggtcaacga taaccgtcag gtagacggtc
     2521 aaaagatcca ctcttctacg atgaacaata aacgtgcgca aaatacagaa tcaacggtgg
     2581 tacaactaaa caatggagat gttaaactct ttatgcgtgg tttgactgga gatcttcagg
     2641 ttgctacaag taaagacgga ggagtgactt gggagaagga tatcaaacgt tatccacagg
     2701 ttaaagatgt ctatgttcaa atgtctgcta tccatacgat gcacgaagga aaagaataca
     2761 tcatcctcag taatgcaggt ggaccgaaac gtgaaaatgg gatggtccac ttggcacgtg
     2821 tcgaagaaaa tggtgagttg acttggctca aacacaatcc aattcaaaaa ggagagtttg
     2881 cctataattc gctccaagaa ttaggaaatg gggagtatgg catcttgtat gaacatactg
     2941 aaaaaggaca aaatgcctat accctatcat ttagaaaatt taattgggaa tttttgagca
     3001 aaaatctgat ttctcctacc gaagcgaaag tgaagcgaac tagagagatg ggcaaaggag
     3061 agatgggcaa aggagttatt ggcttggagt tcgactcaga agtattggtc aacaaggctc
     3121 caacccttca attggcaaat ggtaaaacag cgactttcct aacccagtat gatagcaaga
     3181 ccttgttgtt tgcagtagat aaggaagata tcggacagga aattattggt atagctaaag
     3241 gaagcatcga aagtatgcat aatcttcctg taaatctagc aggtgccaga gttcctggcg
     3301 gagtaaatgg tagcaaagca gcggtgcatg aagttccaga atttacaggg ggagttaatg
     3361 gtacagagcc agctgttcat gaaatcgcag agtataaggg atctgattcg cttgtaactc
     3421 ttactacaaa agaagattat acttacaaag ctcctcttgc tcagcaggca cttcctgaaa
     3481 caggaaacaa ggagagtgac ctcctagctt cactaggact aacagctttc ttccttggtc
     3541 tgtttacgct agggaaaaag agagaacaat aagagaagaa ttctaaacat ttgattttgt
     3601 aaaaatagaa ggagatagca ggtttttaag cctgctatct ttttttgatg acattcaggc
     3661 tgatacgaaa tcataagagg tctgaaacta ctttcagagt agtctgttct ataaaatata
     3721 gtagattgaa ataagatgtg aacaactcta tcaggaaagt caaattaatt
//
LOCUS       DAVKQB010000092         3638 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKQB010000092 DAVKQB010000000
VERSION     DAVKQB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.092"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..177
                     /locus_tag="VW528_002065"
     CDS             <1..177
                     /locus_tag="VW528_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912818.1"
                     /translation="AEALLEKVTDSSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDS
                     LLALLKESQPTPIQ"
     gene            387..3506
                     /gene="phtE"
                     /locus_tag="VW528_002066"
     CDS             387..3506
                     /gene="phtE"
                     /locus_tag="VW528_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9912819.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQISQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLKEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1360 a          592 c          700 g          986 t
ORIGIN      
        1 gcggaggcct tgctagaaaa agtaacagat tctagtatta gacaaaatgc tgtggagaca
       61 ttgactggtc taaaaagtag tcttcttctc ggaacgaaag ataataacac tatttcagca
      121 gaagtagata gtctcttggc tttgttaaaa gaaagtcaac cgactcctat acagtagtaa
      181 aatgaatgga gcatatttta tggagaagta acctttcgtg ttacttctct tttttagaaa
      241 aacgtaacag aaacttgaca aaagtaattt taaatagtaa actatttact ggttaattaa
      301 atggttaaat aaccggttta gaaaactatt taataaagta aaagaagttg agaaaaaact
      361 tcatcattta ttgaaatgag ggatttatga aattcagtaa aaaatatata gcagctggat
      421 cagctgttat cgtatccttg agtctatgtg cctatgcact aaaccagcat cgttcgcagg
      481 aaaataagga caataatcgt gtctcttatg tagatggcag ccagtcaagt cagaaaagtg
      541 aaaacttgac accagaccag attagccaga aagaaggaat tcaggccgag caaattgtaa
      601 tcaaaattac agatcagggc tatgtaacgt cacacggtga ccactatcat tactataatg
      661 ggaaagttcc ttatgatgcc ctctttagtg aagaactctt gatgaaggat ccaaactatc
      721 aacttaaaga cgctgatatt gtcaatgaag tcaagggtgg ttatatcatc aaggtcgatg
      781 gaaaatatta tgtctacctg aaagatgcag ctcatgctga taatgttcga actaaagatg
      841 aaatcaatcg tcaaaaacaa gaacatgtca aagataatga gaaggttaac tctaatgttg
      901 ctgtagcaag gtctcaggga cgatatacga caaatgatgg ttatgtcttt aatccagctg
      961 atattatcga agatacgggt aatgcttata tcgttcctca tggaggtcac tatcactaca
     1021 ttcccaaaag cgatttatct gctagtgaat tagcagcagc taaagcacat ctggctggaa
     1081 aaaatatgca accgagtcag ttaagctatt cttcaacagc tagtgacaat aacacgcaat
     1141 ctgtagcaaa aggatcaact agcaagccag caaataaatc tgaaaatctc cagagtcttt
     1201 tgaaggaact ctatgattca cctagcgccc aacgttacag tgaatcagat ggcctggtct
     1261 ttgaccctgc taagattatc agtcgtacac caaatggagt tgcgattccg catggcgacc
     1321 attaccactt tattccttac agcaagcttt ctgccttaga agaaaagatt gccagaatgg
     1381 tgcctatcag tggaactggt tctacagttt ctacaaatgc aaaacctaat gaagtagtgt
     1441 ctagtctagg cagtctttca agcaatcctt cttctttaac gacaagtaag gagctctctt
     1501 cagcatctga tggttatatt tttaatccaa aagatatcgt tgaagaaacg gctacagctt
     1561 atattgtaag acatggtgat catttccatt acattccaaa atcaaatcaa attgggcaac
     1621 cgactcttcc aaacaatagt ctagcaacac cttctccatc tcttccaatc aatccaggaa
     1681 cttcacatga gaaacatgaa gaagatggat acggatttga tgctaatcgt attatcgctg
     1741 aagatgaatc aggttttgtc atgagtcacg gagaccacaa tcattatttc ttcaagaagg
     1801 acttgacaga agagcaaatt aaggctgcgc aaaaacattt aaaggaagtt aaaactagtc
     1861 ataatggatt agattctttg tcatctcatg aacaggatta tccaagtaat gccaaagaaa
     1921 tgaaagattt agataaaaaa atcgaagaaa aaattgctgg cattatgaaa caatatggtg
     1981 tcaaacgtga aagtattgtc gtgaataaag aaaaaaatgc gattatttat ccgcatggag
     2041 atcaccatca tgcagatccg attgatgaac ataaaccggt tggaattggt cattctcaca
     2101 gtaactatga actgtttaaa cccgaagaag gagttgctaa aaaagaaggg aataaagttt
     2161 atactggaga agaattaacg aatgttgtta atttgttaaa aaatagtacg tttaataatc
     2221 aaaactttac tctagccaat ggtcaaaaac gcgtttcttt tagttttccg cctgaattgg
     2281 agaaaaaatt aggtatcaat atgctagtaa aattaataac accagatgga aaagtattgg
     2341 agaaagtatc tggtaaagta tttggagaag gagtagggaa tattgcaaac tttgaattag
     2401 atcaacctta tttaccagga caaacattta agtatactat cgcttcaaaa gattatccag
     2461 aagtaagtta tgatggtaca tttacagttc caacctcttt agcttacaaa atggccagtc
     2521 aaacgatttt ctatcctttc catgcagggg atacttattt aagagtgaac cctcaatttg
     2581 cagtgcctaa aggaactgat gctttagtca gagtgtttga tgaatttcat ggaaatgctt
     2641 atttagaaaa taactataaa gttggtgaaa tcaaattacc gattccgaaa ttaaaccaag
     2701 gaacaaccag aacggccgga aataaaattc ctgtaacctt catggcaaat gcttatttgg
     2761 acaatcaatc gacttatatt gtggaagtac ctatcttgga aaaagaaaat caaactgata
     2821 aaccaagtat tctaccacaa tttaaaagga ataaagcaca agaaaactca aaacttgatg
     2881 aaaaggtaga agaaccaaag actagtgaga aggtagaaaa agaaaaactt tctgaaactg
     2941 ggaatagtac tagtaattca acgttagaag aagttcctac agtggatcct gtacaagaaa
     3001 aagtagcaaa atttgctgaa agttatggga tgaagctaga aaatgtcttg tttaatatgg
     3061 acggaacaat tgaattatat ttaccatcgg gagaagtcat taaaaagaat atggcagatt
     3121 ttacaggaga agcacctcaa ggaaatggtg aaaataaacc atctgaaaat ggaaaagtat
     3181 ctactggaac agttgagaac caaccaacag aaaataaacc agcagattct ttaccagagg
     3241 caccaaacga aaaacctgta aaaccagaaa actcaacgga taatggaatg ttgaatccag
     3301 aagggaatgt ggggagtgac cctatgttag atccagcatt agaggaagct ccagcagtag
     3361 atcctgtaca agaaaaatta gaaaaattta cagctagtta cggattaggc ttagatagtg
     3421 ttatattcaa tatggatgga acgattgaat taagattgcc aagtggagaa gtgataaaaa
     3481 agaatttatc tgatctcata gcgtaaggaa tagcagtaga aaaagtctga atcaaaaatg
     3541 aagttctctc aaaagttaga aataaaactc tgactttggg agaatttcat tttattatta
     3601 atatataaaa tttcttgaca tacaacttaa aaagaggt
//
LOCUS       DAVKQB010000093         3545 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKQB010000093 DAVKQB010000000
VERSION     DAVKQB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.093"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            76..1335
                     /locus_tag="VW528_002067"
     CDS             76..1335
                     /locus_tag="VW528_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9912820.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1411..1546
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1585..2085
                     /locus_tag="VW528_002068"
     CDS             1585..2085
                     /locus_tag="VW528_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9912821.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2161..2529
                     /gene="rplL"
                     /locus_tag="VW528_002069"
     CDS             2161..2529
                     /gene="rplL"
                     /locus_tag="VW528_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9912822.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            2776..>3545
                     /locus_tag="VW528_002070"
     CDS             2776..>3545
                     /locus_tag="VW528_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9912823.1"
                     /translation="MSLRNKIEQHIKELEGGKFQKLGDAYLSRKYNFNIVSLGSQEGT
                     DKTTKGIPDSYAVENGKYVYIMYGTHKSVISKLEGDIQSVKKKILEENIAEDKVGRLI
                     CCHTSSNITIKQKEDLEKMAEPYHLELIGINEIANDLTKIDFQYLAKEYLSISESTEQ
                     VWSINDFIRIHDESKTNAPISNDYIGDVSEIINTIKSSEKRIFLISAKPGTGKTRLAI
                     EICSLLDRNKYNIICVKSNNQDIYQDVKRNLNLHKENIV"
BASE COUNT         1199 a          607 c          732 g         1007 t
ORIGIN      
        1 aaccacacct acggcaaagt gaatctgaag tggtttgaag agagtacaac ttgtctttta
       61 gaaaaggagc ctataatgaa agtctttcag catgtaaata tcgtgacttg tgatcaaaat
      121 ttccatgttt atcttgatgg aatcttagca gtcaaggatt ctcaaatcgt ctatgtcggt
      181 caagataagc cagcgttttt agaacaagct gagcagatta tagactatca gggagcttgg
      241 attatgcctg gtttggtcaa ttgtcacacc cattctgcaa tgacaggtct gagagggatc
      301 cgagatgaca gcaatctcca tgaatggctc aatgactata tctggccagc agaatctgag
      361 tttactcccg acatgactac caatgcggtt aaagaagccc taacagagat gctccagtca
      421 ggaacaacaa cctttaacga tatgtataat cccaatggtg tggatatcca gcaaatttat
      481 caggtggtga aaacttccaa gatgcgttgt tatttttctc cgactctctt ttcttcagag
      541 acagaaacaa ctgctgagac tataagcaga actcgatcca tcatagatga aatcttaaaa
      601 tataaaaatc caaatttcaa ggttatggta gcacctcatt ctccgtatag ctgcagtaga
      661 gacttgctgg aagcgagttt ggaaatggca aaagagctaa atattccgct ccatgtccat
      721 gtggcggaga ccaaggagga gtcaggaatt atcctcaaac gatacggcaa acgccccctt
      781 gcttttcttg aagaactggg ttatttagat catccgtccg tatttgctca cggggtcgaa
      841 ttaaacgaga gagaaattga acgcttggca tcttctcaag tggctatcgc ccacaatcct
      901 atcagtaacc tcaaactggc atcaggaatt gctccaatta tccagctcca aaaagcggga
      961 gtagcagtcg gaattgcgac tgactcggtt gcttccaata acaatctaga tatgtttgag
     1021 gaaggaagga ctgcagctct tcttcagaag atgaaaagtg gggatgccag ccagtttcca
     1081 atcgaaacag ctctcaaggt actgacaatc gaaggggcta aggtccttgg aatggaaaat
     1141 cagataggaa gtctggaagt cggcaagcaa gcagattttc tggtcattca accacaaggg
     1201 aaaattcatc tccaacctca ggaaaatatg ctgtctcacc tggtttatgc agttaaatct
     1261 agtgatgtag atgatgttta tatcgccgga gaacaggttg ttaagcaagg tcaagtcctg
     1321 acagtagaac tttaaaagaa aaattacgaa aaattttaaa aaaagtttgc aaaaatcttg
     1381 cattcttttt ttgactatgc tatacttata tacggtttaa aaaaactgcc taagacagta
     1441 ggggagctcg actcataaat atcctaccga ggacaaaacg tatcatgtaa aaagaagcgt
     1501 attgtacttt cgtgtctagg tttgggcgcg tttttctttt tgaaaaattc cccaagcaaa
     1561 ataattacgg aggtgaacac actaatgagt gaagcaatta ttgctaaaaa agcggaacta
     1621 gttgacgtag tagctgaaaa aatgaaagct gctgcatcta tcgtcgttgt agacgctcgt
     1681 ggtttgacag ttgagcaaga tacagttctt cgtcgtgagc ttcgtggaag cgaagttgag
     1741 tataaagtta ttaaaaactc aatcttgcgt cgtgcagctg aaaaagctgg tcttgaagat
     1801 cttgcatctg tatttgttgg accatctgca gtagcatttt ctaatgaaga tgttatcgca
     1861 ccagcgaaaa tcttgaacga cttttctaaa aacgctgaag cacttgaaat taaaggtggt
     1921 gcaatcgaag gcgctgtcgc atctaaagaa gagattcttg cacttgcaac tcttccaaac
     1981 cgcgaaggac ttctttctat gctcctttct gtacttcaag cgccagtgcg caacgttgct
     2041 cttgcagtca aagcggttgc agaaagcaaa gaagacgcgg cttaatctta agctacacag
     2101 cgtagcctag ctacgaaaaa aactattata aaatttaaaa cttatttgga ggaaataaca
     2161 atggcattga acattgaaaa cattattgct gaaattaaag aagcttcaat ccttgaattg
     2221 aacgaccttg taaaagctat cgaagaagaa tttggtgtaa ctgcagctgc tcctgtagct
     2281 gttgctgcag ctgatgcagc tgatgctggt gctgctaaag attcattcga cgttgaattg
     2341 acatctgcag gcgacaaaaa agttggcgtt atcaaagttg tacgtgaaat cactggtctt
     2401 ggtcttaaag aggctaaaga acttgttgac ggtgcaccag cacttgttaa agaaggcgtt
     2461 gcaactgcag aagctgaaga aatcaaagct aaattggaag aagctggagc ttcagttact
     2521 cttaaataat agagcaacta cattagtagc ttaaaaacat gattaaaccg ctattcttag
     2581 gagtagcggt ttttcttttt gtttaaaaag gggcaaactt tttatatagt tgtttaggaa
     2641 ataaataatt tttgtataat tggtttacta ctctaaatta acgtagtaca agagtttgtt
     2701 aattaaacta taccttatgg ttttgactag tgtgatataa ttatgttgaa atacttatct
     2761 ggaaaggttt ttataatgag tttaagaaat aaaattgagc aacatattaa agagctagag
     2821 ggagggaagt ttcaaaaact aggtgacgcg tacctaagta gaaaatacaa ttttaatatt
     2881 gtttcattag gctcacagga aggaacagat aaaacaacta aagggatccc agattcttac
     2941 gctgtcgaaa atggtaaata tgtttatatt atgtatggca cccataagtc tgtaatatct
     3001 aaattagaag gagacattca gtcagttaag aaaaaaattt tagaggaaaa tattgctgaa
     3061 gataaagttg ggagacttat ttgttgtcat acttcttcaa atataacaat taaacaaaaa
     3121 gaagatctag aaaaaatggc tgagccttat catctagaat tgattggaat taatgaaatt
     3181 gctaatgatc ttacaaagat agattttcaa tatttagcaa aagagtatct atcaatcagt
     3241 gaatctacag agcaagtttg gtctatcaat gacttcataa gaattcatga tgagagtaaa
     3301 actaatgctc ctatatcaaa tgactatatt ggagatgtaa gtgaaattat aaataccatt
     3361 aaatcaagtg aaaaacgaat cttcttaata tctgctaagc ctggaacagg gaaaactaga
     3421 ttagcaattg aaatttgctc tttgctggat cgaaataaat ataacattat ttgtgtcaag
     3481 agcaataatc aggatattta ccaagatgta aaacgcaatc taaatttaca caaagaaaat
     3541 attgt
//
LOCUS       DAVKQB010000094         3484 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKQB010000094 DAVKQB010000000
VERSION     DAVKQB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.094"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(180..791)
                     /gene="rpsD"
                     /locus_tag="VW528_002071"
     CDS             complement(180..791)
                     /gene="rpsD"
                     /locus_tag="VW528_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9912824.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(986..2038)
                     /locus_tag="VW528_002072"
     CDS             complement(986..2038)
                     /locus_tag="VW528_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9912825.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2035..2733)
                     /locus_tag="VW528_002073"
     CDS             complement(2035..2733)
                     /locus_tag="VW528_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9912826.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQI"
     gene            complement(2900..>3484)
                     /locus_tag="VW528_002074"
     CDS             complement(2900..>3484)
                     /locus_tag="VW528_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEU9912827.1"
                     /translation="PWSDNGDAKNPALSPLGENVKTKGQYFYQLALDGNVAGKEKQAL
                     IDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSN
                     SGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPN
                     TGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT          954 a          782 c          676 g         1072 t
ORIGIN      
        1 attataccac attgtgtact atttttggtt cattttacta tatcactttg gactatatcg
       61 gtctagttat gtctcaacga ttatagctgg atactaagag aaatcaaaaa agcactaagg
      121 gagaacgtcc caaagtgctt aatatcaagg cctcaaagcc tgtcttattc aataaaatat
      181 tacaacatct tgttgtagaa ttcaacgaca agtgcttcgt tgatttctgg gttgatttcg
      241 tcgcgttctg gcaagcgagt caatgaacct tccaactttt cagcgtcgaa tgatacgaat
      301 gctggacgtc caagagtagc ttctactgct tcaaggattg ctggaacttt caatgatttt
      361 tcacgaactg agatcacttg acctggagtt acgcggtatg atgggatatc aacgcgtttc
      421 ccgtcaacaa ggatgtgacc gtggtttacg aattgacgag cttgacgacg agtagtcgcg
      481 agaccaagac ggtaaacaac attatccaaa cgacgttcca aaagaagcat aaagttgaaa
      541 cctaggattc cacctttgat ttttgtagct tgtacgaaca agttacggaa ttgtttttca
      601 cctacaccgt aagtgaaacg aagtttttgt ttttcagcca attgcaaacc atattctgac
      661 aatttagaac ggttgtttgg tccgtgttgt cctggtacgt agttacgacg tgccaattct
      721 ttacctgtac ctgtaagtga aaggccaagg cgacgagctt gtttccaaga tggtcctgta
      781 taacgtgaca tatgaatgtc ctcctgatat aaataatatt tcggtggaaa tagtcactta
      841 gaaagccctg attcgtgcag atgcccttcg cctaaacagc caaggttact tgtcataaga
      901 cacctgttga cgagcttcat gctttcctgc tgatatttca cacaaaatcc attttaccat
      961 gaatagctgg atttgtaaag gggttttagg ctttattttc actaccagag aggttgagaa
     1021 cgagggtaaa ggtacttcct agaccgtact ggctgctgac tgtgatttcc ccacccaatt
     1081 gatgggccaa ttcacgcgca atcgcaagtc ctaatccatg accacctgtc ttcatgttac
     1141 gcgaagtttc gacacgataa aggcgtttga aaatattttc caaatcctct ggggcaatgc
     1201 cctgcccttc atcggtcaca ctgattgaaa gctggtcctt ctccagctta gccaccactt
     1261 ccagcttggt tcctggagca gaatatttaa aagcgttatc gaccagattc accaagatac
     1321 gagaaagctt agcataatct ccctcaatcc gggcagactc tgggattacc tgcaagtgga
     1381 catctcttct ctcctgctca atcaaaaact gaaattcact catgcactca attaagagct
     1441 tgtccagaaa aatactgtct ttactggtag tttccacctg atttctagct gtgtttaggg
     1501 tcaaaaaatt caactcctca accagtttat tgagcctctc cgtctggcgt ccaatggttg
     1561 ctagataatg agcttgctcc gactccttga taatcccatc caaaatccct tctaccgtcg
     1621 cttggatcga agtgataggg gtcttaatat catgcgacaa ctgggcaatc atcaagccct
     1681 tttctcgttc gctttcttcc aaggaatcaa agcttacctg caaatcatgg gacatctcat
     1741 taaaagtttg ccctaattgc tgaaattcta caggaccttg aacctccaaa tttgaaggaa
     1801 aatccttggc cgctacccgc ttggcatgct ccttgagttt gcccaacgac gtaaagactg
     1861 gcaataggag aaagagacta atcccagcac cgacaaggct ggcaacgatg gtcatcccaa
     1921 gcaaaaagta aatctcgcct ttcgcaatca gcattttttg aacagcccaa aaaacaacca
     1981 aaatggttaa gagggttgaa ataatatatc caaccaaaat ataacttttt agtttcatat
     2041 ttgtcctctc ggtttctcta tcttatatcc caacccccaa actgtcttaa tagtgggagt
     2101 ttggtcacta ctatattttg ccagctcctg tcgaagagca tggatatgca cattcaaggt
     2161 attggtgtca tccacgtagt cttctttcca gatcttttca tagaggtctg tcttggagaa
     2221 aactcgctct ggattgctag ctaaaatcca cagcaattca aatgatttaa cagttaaatc
     2281 cagcatttct tctcctattt gaacttcatg actactatga ttcattttta gattgccaag
     2341 ggaaatcagc tctgtttcgc ccccacgatg aaggcggcgc aaaatattgt ggacacgcaa
     2401 aaccagctca cgtgggctaa aaggcttagc aataaaatca tctgctccca agctcaggcc
     2461 gtaaatcttg tcctgttcac tggtcttagc agtaataaat aggaaaggct gctctggtga
     2521 taagtattga acctcactga ttaaatcata accatccatc cgaggcatca tgacatctgt
     2581 gataatcaaa tcaatcggtt ttttcttgaa cagctctagc gcttccagtc catcatgggc
     2641 taccaagacc tgataacctg cctgaattaa gtatctctga tgaatatctg tgatttctac
     2701 ctcgtcgtca acgagtaaaa ttgtctttcc catctgtctc tcctttgata aaaacagtgc
     2761 tatactgctt taagtataac actattttta ttgttttacg atgattttaa tctgctattg
     2821 aaagaaagtc ctgattgctg attgagttgt taaactaatc ttagtcagcc cactatccat
     2881 catttatttc cttagctgat tagttttctt cttttttgtt tttcaaacct agaccaccga
     2941 gtaaacctgc aagcgcaagc ccaaggaaac caatacttgc cattgatgtt tgagtctcac
     3001 cagtatttgg tagcatagct ttatcctctg acatcatcgt atcagacatc ttgttagcag
     3061 aagcagccat gttttcacct gccatcgtgt tggtagaact tgtcatggtg tcagcaggca
     3121 tgctatctgt agtacctgta gcatgattgt gattcatcgg agtcacgcca gaaccagagt
     3181 tagaaggtga taatgaacca tttgctgtgt ctgatgtttc tttaacattt atcttaatag
     3241 tgactttttt agttgctacg atgttgtcca aatctggttt accgtctttg ttaccataga
     3301 cattgactgt agcgctgtaa gtttgagtac catttgctcg gaactggtca atgagcgctt
     3361 gtttttcttt gccagctaca tttccgtcca aggctaattg atagaagtat tgacctttgg
     3421 tcttcacgtt ttcacctagt ggagatagag ctgggttttt agcgtcgccg ttatctgacc
     3481 atgg
//
LOCUS       DAVKQB010000095         3464 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKQB010000095 DAVKQB010000000
VERSION     DAVKQB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.095"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            38..598
                     /locus_tag="VW528_002075"
                     /pseudo
     CDS             38..598
                     /locus_tag="VW528_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            703..1051
                     /locus_tag="VW528_002076"
                     /pseudo
     CDS             703..1051
                     /locus_tag="VW528_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(1322..1543)
                     /locus_tag="VW528_002077"
     CDS             complement(1322..1543)
                     /locus_tag="VW528_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912828.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            1508..2137
                     /locus_tag="VW528_002078"
     CDS             1508..2137
                     /locus_tag="VW528_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912829.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            complement(2173..3396)
                     /gene="pepT"
                     /locus_tag="VW528_002079"
     CDS             complement(2173..3396)
                     /gene="pepT"
                     /locus_tag="VW528_002079"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9912830.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
BASE COUNT         1168 a          655 c          664 g          977 t
ORIGIN      
        1 aagacatcat ataaataatt atagaaatga ggtaattatg aaatttaaaa agaaatatat
       61 agcagctgga tctgttgtta tcctttcctt aagtctgtgt gtttatgctc tgaaccaaca
      121 tagctaacag gccaatacag ataaaaatcg tgtttcatat gtaaacagta ataaagacac
      181 taagaagact gaaaatttga ctccagacta ggttagtcaa aaagaaggaa ttcaagcaaa
      241 acaaattgtt attaagatta cagatcaagg ttatgtaact tctcatggcg atcattatca
      301 ctactataat ggtaaagttc cttttgatgc gctctttagt gaagagccat taatgaaagc
      361 ccccaactat cagccgaaag atcaagatat tgtcagttag gttaagggag gatatattat
      421 taaggtagat ggaaagtatt atgtttacct taaagatcaa gctcatgcag aaaatgtacg
      481 aacaaaagat gaaatcaatc gccaaaaaca agaacatggt aaagacgata aaggagctag
      541 tgcagaagta tctgtagcaa aattacaggg acgttatact accgatgatg gttatatata
      601 gtgagttgaa tttagaatag cacatcatag tttctaaatc attgttagaa attactttaa
      661 aatcccctta tcaatttgct catattctat ttcaatctac taatagtacc acatggtggt
      721 cactatcatt tcattcctga gagtgacttg tcggcaggtg aattagcagc cacatatgtc
      781 tttaacccta atgatattgt cagagatata ggtgatgctt atatagtaag acatggggaa
      841 cactatcact atattcctaa gtcatctctt aacaatcccc catcacactc taacacggaa
      901 gaggtaggaa gttcttctag ttctgtatta agtaatccgt cactacatgt ccaccatgag
      961 gaagaagatg gtcatggttt ttgatgccaa tcgaattata agtgaagatt cagaaggttt
     1021 tgtgattcct catggcgacc acaatcacta catcaaggtt caaactaagg gatatgaagc
     1081 ggctttgaaa aacaagattc catccctgca atccaactat caacctggaa cttttgatga
     1141 gaaggcagtc ttggcaaaag tagatcaact tctagctgat agcagaagca tctacaaaga
     1201 cagattgagt tagctttagg tcagtttact gaaagtttga aaaaaataaa agttagttga
     1261 ttgattactt ctctaaattt tgggcacact aaaaagaggt cttgacacct aaactttaaa
     1321 attaaagcat attgaatccg tgctgctgtg cgaagcgaat agcgtcttgg acgaacattg
     1381 tcttatctgg atttcttatt tctgtaaggc cttgtaatct tggattataa ctctcctgtt
     1441 ttaggagtac atggtagata gcaaccaata atctgcgaca gatagcaagg aagaagtaga
     1501 aagtcttttg aacaaggcaa atcaactctt ggaacaaatc catgaagaag gaatcagaca
     1561 atccttggca gaagaagtag aaaatctaaa agctgccaca aacaaggctg atgcagactt
     1621 ggatgaagta aacagtcagg taaaagatgt tttgactcgt atcgctagcg cccttcaaca
     1681 agaaaaggaa aatgctgagc aagattctca gacacttgta ctctatcaaa aactctacga
     1741 tattctcatg tcgcttcact cttatttaga aagtaataat ggatctgatg cggactttga
     1801 taaggtggat gcattattag atcaactttc tgctaatagt aaagacaaaa cagctttact
     1861 tgaatcgaca aaagctattc tagttttgaa tcatcaaatc cagtcaaaag ttagcgcaag
     1921 tgaagaaaca agtccagcaa gaaatgctga agcaaatagt gataacacca gtgctgagaa
     1981 ccaaccaaac gcaactgcag aatctaacat tgaaactgct agcgacgaaa acaaaccaag
     2041 caatacaaga gattctaaac ctgctgaatc aacttcagaa aatgaaacaa cagaatcttc
     2101 tacaactact ggaaatcaag aaaaaccagt agaataaaaa gaaaggcgaa gtagctgagc
     2161 cacctcgtct ttttagcctt tataagctac gatgccaatg atggtatcaa ctgcacgttc
     2221 catagtctga aggctaacgt attcaaaacg tccgtgcata ttttcgccac ctgcaaagat
     2281 attcggagtt gggattccca taaaggaaat cttagagccg tctgttccac cccggattgg
     2341 ttcgataata ggcgtgatac ctagatcttc cataacggct ttagcaatgg taattggagt
     2401 catatctttt tcaatgactt ctttcatatt gtagtactgg tctgtcaagt tgagagtgac
     2461 acggtcgctc ccaagttctt cattcatctt atcagcgata gattgcatgg atgctttacg
     2521 cgcttcaaag gcatcttttt caaaatcacg aatgatgtag cttgcacgca cctcctcaac
     2581 actacctgtc acatccatta gatggtaaaa accttggtaa ccttcagtta actcaggtcg
     2641 gtcattttct ggaagttgat tatgaaaatc aattgctagc tgaagggcat tgaccatctg
     2701 ccctttggca gtaccagggt ggacattacg gccttggaaa tgcaattcag caccagcggc
     2761 tgagaaagtc tcgtactgaa gttcacctag tggaccacca tcaacagtgt aggcaaaatc
     2821 cacatcaaaa tcttctgcat caaatttatt ggcaccaaca ccgatttctt catctggacc
     2881 aaaaccaaca cgaatctcac agtgcttaat ttcaggatga gcagttagat attcaatggc
     2941 tgtcataatt tcagcaattc ctgacttgtc atcagcacct agcaaggttg ttccatctgt
     3001 tgtgatgagt gtttgtcctg gatatttttc aagactcttg aagtcggctg gatcgagttt
     3061 gaaaccagaa ttccctagtt caatcacacc accatcgtag ttttcaatta cctgtggatt
     3121 gactccttca gcattaaaat cagcagtatc catgtgcgat ataaaaccaa tcttacgtgt
     3181 taaagacgga tcgttggctg gcaaggttcc aatagcaaaa ccattcggta gatagtaaac
     3241 attttgcagt ccaacacgtt tcatttcagg aattaggaca tttgttgcga agtcaacctg
     3301 actctgtgta cttggagtag tagtagagtg ttcatcagag cgcgtgttga ccttaacgta
     3361 ggttaagaag cggtccaaga gatttggata agtcataaaa aaactccttt tttatcataa
     3421 aatagaatac aaaacttcgg aaaaatatga tttgagaaat ttta
//
LOCUS       DAVKQB010000096         3427 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKQB010000096 DAVKQB010000000
VERSION     DAVKQB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.096"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..227)
                     /locus_tag="VW528_002080"
     CDS             complement(<1..227)
                     /locus_tag="VW528_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HEU9912831.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITE"
     gene            complement(527..3205)
                     /locus_tag="VW528_002081"
     CDS             complement(527..3205)
                     /locus_tag="VW528_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9912832.1"
                     /translation="MAVFVSLDGIVVEVLDVFSSFDGDSEFFLCKRLKDKSQFVMERS
                     RFEEMFQLQSSHLTTQEKLQLFTSVFAGRYDVYAKNFINEQGKIQYFPSYDYGWKQLP
                     PEKRSFQTLTNSVLKSHFRGETAIGIFPMHLDDSCHFLVLDFDEGDWKEAGLTIRRIA
                     RERQMEAHLEISRSGHGLHIWFFFEEAIPSREARLFGKKLIELAMQESMQLSFDSFDR
                     MFPNQDVLPKGGFGNLIALPFQGEAYHQGRTVFVDEQFQPYEDQWRYLQEIQRVSTAK
                     VALLIQEELGKQELDKGLKIVLSNMIQLEKSSVTPKTLFFLKNMASFSNPEFYLKQAM
                     RQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVVVEDRRKVQRSISVEFKGEL
                     TFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKTKTIILVHNKQLLDQWLDR
                     LNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKLVDVVMIQSLFKLENSQSL
                     LDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPERKNGHEPIVFQRIGEILH
                     TADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWIATDSARNQLILKDILAQV
                     AEGRNILVLVNRIQQIDVFEKLLKEKEVDDCYIISGKTKVRERTSLLETLEQLDKGFV
                     LLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIHRNYKDKSLVRIFDYVDIH
                     VPYLEKMFQKRQVAYRKMDYRAIEGEEKQFVYVDSRYEKVLIEDLAGERQECLLILPY
                     VHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKIKVSFTQSKIVTPILLVNK
                     TIVWYDAMPLLGKVDEMTILRLESASIVSELVAGLR"
BASE COUNT         1096 a          766 c          505 g         1060 t
ORIGIN      
        1 ttttcggtga tgtacaccaa ctccaaagga acgttaataa acatctctgt aaaacgggtc
       61 atattatctc tatttttgag aaaaaaactt ttctcaatat caattggatt tactttatct
      121 ccaatttttc ttccaaaagc tattcccctt ccaaaaagaa gaatatcgat atctttttta
      181 ctttgcgcaa ttacggcatt gtggtttagt attcgtttta tgatcataaa atttcccttt
      241 taaaataaaa ggaacctact taaaaacatc aagaaacttc ttctgatgtg attaaatagg
      301 ttcctgctca cttgtattag tgataacatc cttttatttc aatttttaat ggtgacatga
      361 agcttaaata attaataact ctgtaaccat tttcatttta agatatttca atctatttgt
      421 caagtacttg tagaaatttt tttattttgt gtgattcgaa tgtgataatg gcaacagtct
      481 cactaaagaa atgaaaatca tacatagaaa tttttaaaaa ttttctctat cgtaaacctg
      541 ccactagttc agaaactata ctagctgatt ccaaacgtaa tatggtcatc tcatctacct
      601 tccctaataa tggcattgca tcataccaaa caatagtctt attcaccaaa agaataggcg
      661 ttactatttt tgattgagta aaagacactt taattttctg gctcttcaac tggtctagcc
      721 atgctttatt tgcaaccgtc tctggtatac atatctcaat ttgactaatc ctaaattctt
      781 ttagaaaatt catcagtttt gtctggtgca cataaggtaa aataagcaga cattcctgtc
      841 tttcccctgc taagtcctct atcaacacct tctcatatct actatcaaca taaacgaatt
      901 gtttctcctc accctcaatg gcacgataat ccatctttcg ataagctact tgtcgtttct
      961 gaaacatctt ttctaaataa ggaacatgaa tatccacata atcgaaaata cgcaccaaag
     1021 acttatcctt gtagtttcta tgaatccgac ctgcatactg aattaaatta tttttccaag
     1081 aaaagggtgc tgccaagata agcgtgtcca actgaggtaa gtcaaaacct tcgccaatgt
     1141 attttccagt agacaacaaa acaaaccctt tatctaactg ttctaacgtc tccagtaaac
     1201 tcgttctttc tcggactttg gtttttccgc taataatgta acagtcatca acctctttct
     1261 ctttcaataa cttttcaaag acatctatct gttgaattcg attaactaaa accaagatat
     1321 tccgtccttc tgccacttgg gctagaatat ccttgagaat caactgattc ctcgctgagt
     1381 ctgtagcaat ccaatcactg agctgtataa aattacttgc tttggtcttt tcaatctcca
     1441 aatgaccaaa agaagtgaat cttaattgca attgccgttt aaaatccgtt tccctcttat
     1501 cagcagtatg gagtatctca ccaattctct gaaaaacaat aggctcatga ccattcttac
     1561 gctcaggcgt agccgtcaaa ccgtaaagat acttccctct aaactgagca acaacttttt
     1621 caaacatcaa ggcagagaca tgatgacact catccacaat catcatctca tactcatcca
     1681 aaagactttg actattttcc aacttaaata gagattgaat cataacgaca tcaaccagtt
     1741 tactcagcca tttcttagtc ccaccgtact gtccaacata gccgattacc ttttcacgac
     1801 ctgatgccgt ataacggata gcctcctcct cttcgaaagt caaaaagcag tttaagcgat
     1861 ctagccattg gtctaagagt tgcttattat ggactagaat aattgttttt gttttccgtt
     1921 cagagataag agcagcacct aaaacggtct tcccaaaacc agtctccgca tgaagtaaac
     1981 cattttcttt agaagtcata tctgacaggg ctaactcttg ctcaaaagtg agttctccct
     2041 taaattccac gctaatagac ctttgtacct ttctcctatc ttccacaact acctgcttaa
     2101 atttatcttg caatggatac agcaaacctc ttggcaacca taaataatga tcggattctc
     2161 caaataaata cattctctca ggaatttgat aggttggctg tcgcatagcc tgctttaaat
     2221 aaaattcggg attagaaaat gaagccatat ttttcaagaa aaacagtgtc ttgggtgtca
     2281 cagacgattt ttcaagttgg atcatattgg ataagacgat cttcaacccc ttgtccaatt
     2341 cttgcttgcc taactcttct tggattaaca gtgccacttt agcagttgaa accctctgaa
     2401 tttcttgtag atacctccat tggtcttcat aaggctgaaa ctgttcatcc acaaagaccg
     2461 ttcgcccttg atggtaagct tctccttgaa aaggcaaggc aatcaaattt ccaaatcccc
     2521 ccttaggaag gacatcctga tttggaaaca tgcgatcaaa agaatcaaag gacagttgca
     2581 tactttcctg cattgccagt tctatcagtt tctttccaaa caagcgagcc tctcgactcg
     2641 gaatcgcttc ctcaaagaag aaccaaatat ggagtccgtg acccgaacga gaaatctcta
     2701 aatgggcttc catctggcgt tccctggcta ttcttcgaat ggttaaacca gcttctttcc
     2761 aatctccttc atcaaaatcc agtaccaaaa aatgacagct atcatctaag tgcataggaa
     2821 agataccgat agctgtctcc ccacgaaaat gagatttcaa aacggagttc gtcaatgtct
     2881 ggaaactccg tttttcaggt ggcaactgct tccaaccata atcataggaa ggaaaatact
     2941 gaattttccc ttgttcattg ataaaattct tagcataaac atcataacgt ccagcaaaca
     3001 cggaggtaaa caattgtaat ttttcttgcg tcgtcaagtg actactttga agttggaaca
     3061 tctcctcgaa ccgagagcgt tccataacaa actgactctt gtctttaaga cgtttacaca
     3121 agaaaaactc actatcccca tcaaaagaag aaaagacatc aagcacttct accacaattc
     3181 catccaaaga cacaaaaaca gccataagag tcacctcctt gattcctata ggctgattat
     3241 aacaagaata gctgaaattg tacacgaaaa taaaatccga atagcactca ttttgtatgc
     3301 gactaatagt ccatctcgct ccggaatgta cgaggtaaat agagtttaaa cgagcagtct
     3361 ataaaattca aaaaacgcat attaccaagg ttgttttgaa ctacacccca aaagttagac
     3421 agaaaaa
//
LOCUS       DAVKQB010000097         3401 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKQB010000097 DAVKQB010000000
VERSION     DAVKQB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.097"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..459
                     /locus_tag="VW528_002082"
     CDS             <1..459
                     /locus_tag="VW528_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9912833.1"
                     /translation="RYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLK
                     HHYQIYQLLLFHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLHY
                     SNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(589..1404)
                     /locus_tag="VW528_002083"
     CDS             complement(589..1404)
                     /locus_tag="VW528_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9912834.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(1401..2306)
                     /locus_tag="VW528_002084"
     CDS             complement(1401..2306)
                     /locus_tag="VW528_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9912835.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            2648..3401
                     /locus_tag="VW528_002085"
                     /pseudo
     CDS             2648..3401
                     /locus_tag="VW528_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         1159 a          588 c          651 g         1003 t
ORIGIN      
        1 cgttactgga aactcatcca acaggatagt cgtaaattga gcgataaacg tttttatcgc
       61 cctacttttc gcatgcactt aacaaataaa gaaattcttg acaagatttt aagctattca
      121 gaagacttga aacaccacta tcagatctat caactcttac tttttcactt tcagaataag
      181 gaaccggaga aatttttcgg actcattgag gacaatctta agcaggttca tcctcttttt
      241 cagactgtct ttaaaacctt tctaaaggac aaagagaaaa tcgtcaacgc ccttcaacta
      301 cactattcta acgccaaatt ggaagcgacc aataatctca tcaaacttat caagcgcaat
      361 gcctttggtt ttcggaactt tgaaaacttc aaaaaacgga tttttatcgc cctgaatatc
      421 aaaaaagaaa gaacgaaatt tgtcctttct cgagcttagc tttttttcaa cccactacag
      481 ttgacaaaga gccaatctac aagtcactaa ttttcatata acaaaaaaac gaacaggctc
      541 tttttctgtt attcagaaga gaacttgttc gtttttatct tgattttctt aaaaagctct
      601 atatacatag ggattttctg gtttatcgac tttggtaaag ctgtcgattt ccaaggttgg
      661 aaggttttgt ttgagttctt tatggtagtg attgaccagt tttcctttgg ctgtaatcca
      721 ggtgttgttt tcatactgcc gggtattgcc cttggtcagc aatccataga caccagaatc
      781 tgcgatacag tggataatgc cgaatcggaa cagaaattga ctattggcat gactggggtc
      841 gttatagaca aagcctgtaa actggattgt cttgccctca aattcatctg gatagtcgta
      901 gatagcctcc atgacttcca tatagttttc attagtgatc tgaatactat cttgggataa
      961 gtatttatcc gccgccgttc gcatttcctt ttcataggct gattttgaaa aataagaact
     1021 agtatctggt ttcaaatatt ggcttgtcgt cccttcactt gtctgaatag ctagatccgt
     1081 tccttccgat agggggaaat gataaccttt agcagaaacg gtctgagaat ccaagctaac
     1141 agttgggaaa gttaagccga tgacaagtgg aatagccaga agagaaatac tcgtcatctt
     1201 ggccaatcgg ctgttcagat gactgtgggt tttgacttgc ttcatccaga tatacaattg
     1261 aacaattgcc aagataaagg aaagcaccat ggagatatag gccagatagg aatagtgcat
     1321 gttgatgtac tggtttagtt tgcccgacag atggaggtaa atagtcagtt caaaatagcc
     1381 agctaaaact aaaaatcgaa tcataggatg actccaatca agagagaata ggctaaaacg
     1441 accagagtca caatcgtaat gaagtgacta ataaatcgtg ctttcaagta atttttcatc
     1501 atgagaatat ttttgatatc cagcattggg ccaatgacga gaaaggccag aactggtgcc
     1561 aaaccgaaac tcgagagtag agaagcacct ataaaggcat ccgcctcact acagagcgaa
     1621 agaagaaagg ctaaaagcat caagagcagg atagcaaaaa gaggagtcgc actgatagag
     1681 gtcagaatcc gagtcggaac gtagacctgt attatagaag caaagaggca gccaaatacc
     1741 aaataacgcc ccgtatcaaa aaattcatca atggcctgca caaagacttg aaaaactttt
     1801 tttgcagaac tcaagtaaga aaaatcatgc tcatgacaag ccagacgatt ttctttctga
     1861 atcggttctt gccagaaaaa tcctagaaaa attcctagta ttacagccac aagaatggaa
     1921 cccagagctc gtaatagggc gacatggaag gagttgccaa aggcagaata ggtcgcaaaa
     1981 agaacaatgg gattgataac aggtgctgtc acaagaaaag gaacggccgt gtaacttgga
     2041 accttttttt ccagaaaacg attgatgatg gggacgattc cacattcaca agaagggaaa
     2101 agtataccga caaaggtccc aaaaaagatt ctcccccaac gatttcgagg gagaaaatga
     2161 taaaccttgt caggtgtgat ataaacttca atcagccctg agacaatgct tcctatcaga
     2221 acgaagggaa gggcttcaat gataatggag agaaaaatgg ctccagtttg aagtacacta
     2281 gatgggagtt gttgaaaagc agtcatctag tttttctttt ctgctttgac ttttttctct
     2341 ttgtcatcag ggaaagtaac ttgtttcaaa tcagggagtt tagcaaactc ttcaaacatc
     2401 ttgtccaagt cgtctcgttt tgtaaatttg acagccatag gtgtacctcg attctaaatt
     2461 ctacctctat tatactatta ttttgcggaa aagggaaaag ttaaaatttc aaattgagta
     2521 gctaagagat aatatagctt gatattacct gatatatatc ataattgtgc tataattaaa
     2581 taaaaagaaa atcattatca gaaaattatg ggaggtagtc aaactgactt actcaattag
     2641 tgttgttttg ttacatcaag gagataaaaa tagtttatta gagtctttgt caatagtgga
     2701 gagacaaaca ttaaaagata tagaagttat tattgtaaat caatctacag aggagatagc
     2761 ttccattagc caacagatga atctatcatt taatattaag attatagatg agaattcttt
     2821 tgctgagttg tctgatatga ttacaggaga ttatattacg tttctatctt caaatgattc
     2881 attatttgat tggacatttg aaaagatgta tcatgcgata aaattatccg attctgatgt
     2941 tgttcttggt cattttgctg atttggttga tggagtattt tacttttatc ctttgggggg
     3001 gggataattg gttgacagaa aatttaacga atgttcaagt tttacaaaag atggatgata
     3061 cagatcatcg aattagaaaa caatatcgct cattatttgg aaaattattc aatagtaagc
     3121 ttttacgaaa aataaatcaa tttgatataa acaaccttat ttggagactt tatattcact
     3181 ctaaaactgc aacctatatt aatttcccga catatatcta taaacctgtt atagatgcta
     3241 aacctctaaa agactcctac aaaatcatat tagaaaatta tgaaaataga atcaaagatt
     3301 gcgccgtctt agaaaactat gatatagaaa gagcgaaaaa acaatatatt caagaattgg
     3361 caaattttag tgcttggtta aaaaatgacg gtgaatattt a
//
LOCUS       DAVKQB010000098         3112 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKQB010000098 DAVKQB010000000
VERSION     DAVKQB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.098"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(295..828)
                     /locus_tag="VW528_002086"
     CDS             complement(295..828)
                     /locus_tag="VW528_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9912836.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(917..1693)
                     /gene="recX"
                     /locus_tag="VW528_002087"
     CDS             complement(917..1693)
                     /gene="recX"
                     /locus_tag="VW528_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9912837.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDENIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1731..3086
                     /gene="rlmD"
                     /locus_tag="VW528_002088"
     CDS             1731..3086
                     /gene="rlmD"
                     /locus_tag="VW528_002088"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9912838.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT          993 a          545 c          560 g         1014 t
ORIGIN      
        1 gtggaaatac aaaaaaatgt ttttgatacg aagttgacct gtatttttta tactaatcat
       61 tttcgtattt tttgtattaa acgatataag tttgttgtaa acttacaagg aataaagaca
      121 ttaaaaaata acagtatatc tatttgtttt atatatttta cgaattttgc ataaatctct
      181 ttctagtaat gtgttgtaac tctgctataa tagatttatt cctttttgtg tttacacaat
      241 ttattttata ctaccaaaaa aaggtcagga ttttgttcct gacctttgac aactttaccg
      301 attctttagt tctacatagc gcttgtacca aatgtttaca taggcttctg agaaaggacc
      361 acgtccattg ttaatccaat caacaagaat tttgacatgt tcttttaaaa tatagtccaa
      421 gtcatcagaa taattcattt tgcgtttgtg acgctcgtac tcttcaacgt ccaagagacg
      481 tttttcccca tctgtaaaaa ttttaacatc caaatcgtaa tcaatatact tcagtgcttc
      541 ttcatccaga tagtaggggc tagccatatt gcaatagtaa gaagttccat tatcacgaat
      601 catggcaatg atattaaacc aatatttctt gtgaaagtaa acaatagccg gttctcgagt
      661 gacccaacga cgaccatcac tttcggtaac aagtgtatga tcgttgacac caataatggc
      721 gttttctgtt gtttttagta ccatggtgtc ccgccaagtt cggtggagac tcccatcatg
      781 cttataactt tgaattgtaa taaagtcgcc ttcttttgga agcttcataa ctaaccaact
      841 ttctacaatt tataagttta tcatttacta ttgtaccata aaattaccta aaatctgtga
      901 atttcacttg gaaatattaa agatattctc taagagcgct tgctatatcc gaaaaatcgt
      961 agccctttcg tgctaaaact tgagttaaac gctgcttcag ttcgtatcct tcatactttc
     1021 gagcatactt agtatattgc ttatcaagtt ccttgaagat gagttcttga gtcgtttctt
     1081 ggtcaacttg gctatctaag tcgtcaaagg caattttagc atcagaataa gagaagccct
     1141 tgttagtcaa gttctggata atcttatctt gcagggcacg agctggaagt tttccctcat
     1201 attttttcaa tagtttattg gctacacgtt gagcaacttc agaaaaatca aattctttca
     1261 agttctcttc tatagtagat tttgaaatcc ctttttgagc tagtttctga gtcagcacat
     1321 aaggaccctt gtctcctgaa agttgattgg tattgatgat agcataagcg tactgaccat
     1381 cattaatcca cttatcttct ttaagattag caataacttg agaaacgatg ttttcatcaa
     1441 tatcgtattt tttcagatat tctctgactt ctttttcagt gcgtgcttta aaggataagt
     1501 ggtagagggc cagattctta ccataagaaa attgagcaaa gtcttgaatc tctttcaatt
     1561 cctcttcgct tatcacctta tctctcgata acataaaacg aacaattgta tcttcggtga
     1621 tatagcattt gtcgccatta tcaagctcca tcagatagag tctttttttc ttttcaagtt
     1681 ttgtgatttt catagctcta ttataactca aaatgtgata agataggggt atgaatctga
     1741 aagtgaaaca aaaaatacca ttaaaaatca aacgcatggg aattaatggt gagggaatcg
     1801 gcttttacca aaaaacatta gtctttgtac ctggagctct caaaggcgaa gatatctatt
     1861 gtcagattac ttctattaga cgcaactttg ttgaagcaaa attactgaag gtcaacaaga
     1921 agtctaaatt tcgaattgtg ccatcttgta ctatttataa tgaatgcgga ggctgccaaa
     1981 tcatgcacct gcattatgat aagcagctgg agttcaagac ggacttactt catcaagcgc
     2041 tgaaaaaatt tgctcctgca ggatatgaaa attatgaaat tcgtccaact attggaatgc
     2101 aggaaccaaa atattacaga gctaagttac aatttcagac tcgaaaattt aaaaatcagg
     2161 tcaaggcggg cttatatgca caaaactctc actatttagt agagttgaaa gactgcctgg
     2221 tacaagataa ggaaacccaa gtgattgcta atcgcttagc agaattactt acttatcacc
     2281 agattccaat cacggatgag agaaaagttc taggtgtccg tactattatg gtccgacgcg
     2341 cgagaaagac cggacaggtt cagattatta ttgttacaaa ccgccagctt aatttaactc
     2401 aattggtaaa agagttggtt aaagatttcc cagaagttgt gacagtagct gttaatacaa
     2461 atacagctaa aaccagtgag atatatggtg aaaagacaga gattatctgg gggcaagaga
     2521 gtattcaaga aggtgtactc aattatgaat tttcactatc ccctcgagct ttttatcaac
     2581 taaatcctga gcaaacagaa gtcctctata gcgaagcagt aaaagcgctg gatgttgata
     2641 aagaagacca tttgattgac gcttattgtg gagttggaac gattggattt gcctttgcaa
     2701 agaaagtaaa aacactcaga ggtatggata ttattccaga agctattgaa gatgccaagc
     2761 gaaatgctaa aagaatggga tttgacaata ctcattatga agctggaacg gcagaagaga
     2821 ttattcctcg ttggtacaag gaaggctacc gagcagatgc tttgattgtt gacccaccac
     2881 gtacaggtct ggatgataag ttattagata ctattcttac ttatgtacca gaaaaaatgg
     2941 tttatatttc ttgtaatgtt tcgaccttgg ctcgtgattt ggtacgctta gtagaagtct
     3001 atgatcttca ttatatccag tcggtcgata tgttcccaca tacagctcga actgaagctg
     3061 ttgtaaaatt aataaaaaaa gtttaaaaaa gtagttgaca aagtttgaaa ag
//
LOCUS       DAVKQB010000099         3033 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKQB010000099 DAVKQB010000000
VERSION     DAVKQB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.099"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            30..1055
                     /locus_tag="VW528_002089"
     CDS             30..1055
                     /locus_tag="VW528_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9912839.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1045..2052
                     /locus_tag="VW528_002090"
     CDS             1045..2052
                     /locus_tag="VW528_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9912840.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2052..>3033
                     /locus_tag="VW528_002091"
     CDS             2052..>3033
                     /locus_tag="VW528_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9912841.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFVLLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLL"
BASE COUNT         1029 a          433 c          527 g         1044 t
ORIGIN      
        1 tagacttgaa atatgtttaa ggagttttta tgaaaaacaa attttttcta atagctattt
       61 tagctatgtg tatagttttt agcgcttgtt cttctaattc tgttaaaaat gaagaaaata
      121 cttctaaaga gcatgcgcct gataaaatag ttttagatca tgctttcggt caaactatat
      181 tagataaaaa acctgaaaga gttgcaacta ttgcttgggg aaatcatgat gtagcattag
      241 ctttaggaat agttcctgtt ggattttcaa aagcaaatta cggtgtaagt gctgataaag
      301 gagttttacc atggacagaa gaaaaaatca aagaactaaa tggtaaagct aacctatttg
      361 acgatttgga tggacttaac tttgaagcaa tatcaaattc taaaccagat gttatcttag
      421 caggttattc tggtataact aaagaagatt atgacactct atcaaaaatt gctcctgtag
      481 cagcatacaa atctaaacct tggcaaactt tatggagaga tatgattaaa attgattcaa
      541 aagccttagg tatggaaaaa gaaggtgatg agttaatcaa aaatactgaa gctcgtatat
      601 ccaaagaatt agaaaaacat ccagaaatca aaggaaaaat caaaggaaaa aaagtattat
      661 ttactatgat taatgctgca gatacatcaa aattctggat ttatactagc aaagatccaa
      721 gagcaaatta tttaacagat ttaggtctag ttttccctga atcattaaaa gaatttgaga
      781 gtgaagatag ttttgcaaag gaaatttctg cagaagaagc aaataagata aatgatgctg
      841 atgtaatcat aacttatggt gatgataaaa ctcttgaagc tttacaaaaa gatcctcttt
      901 taggtaaaat aaatgcaatt aaaaatggtg ccgttgctgt aattccagat aatacaccgt
      961 tagcagcctc atgcactcca acaccacttt caataaacta tactattgaa gaatacctaa
     1021 atcttttagg aaatgcatgc aaaaatgcga aataaaaaac aaataaacct aggcataatt
     1081 tttgtaatct gcctaggtct tcttattaca atatttttgt cattaaagct tggaacaaaa
     1141 gaaattaata tcagagattt tttagcagct tttggaatgg gtaatacaaa tgatgatttt
     1201 attaaatcaa ttatatataa aagaatacct agaactattt ttgcaatttt agcaggttct
     1261 agtcttgcca taagcggtgt attgatgcaa tcagttacta gaaacccaat agctgatcca
     1321 ggtatactcg gtataaacac aggagcaagt cttagtgtag taattggtct ttctttttta
     1381 ggaatttcat caagcataag ccatataagt tttgcaatca ttggtggctt agtaagtgca
     1441 atttttgtat acgcgattgc tgtaagcgga aaagcaggcc ttacccctat aaaacttgcc
     1501 ttatcaggaa cttgtgttag tatggcttta agcagttttg taagtttttt aattttaccg
     1561 aataataacg tcttagacaa atttagattt tggcaaatag gtagccttgg agcagctaca
     1621 ttatcttcta tatctacact actacctttt ataattttag gtcacttgat agctatattt
     1681 atttcatcag atttaaacgc tttagctatg ggtgatgaaa tggctgttgg tcttggagtt
     1741 aatgttaata ggataagatc acttgcaata attgcaagtg tgcttttatg ttcaagtatt
     1801 actgcaattg gtggacctat tggcttcgta ggtcttatag ttcctcactt ttgtggctta
     1861 tttataagca aagatatacg cacaatgacc atttcttcat cttttatagg tgcagagctc
     1921 ttgcttatat gtgatataat cggccgtatg ttaggtaaac caggtgaaat tgaagtaggg
     1981 ataattactg caataatcgg gggtccagta cttatttatg taactatgaa aaatagaggg
     2041 gttaataact aatgcaaaat ttaattatag gtattcaaaa aagaaaaaat agaataacac
     2101 tattttcctc actattttta ttaataataa tcagtctatc atttttcgtt ttacttatcg
     2161 gagatgaaag ttattctttt tcaactttga ttaaagtctt aaatagtgaa actgttcctg
     2221 gagctagttt ttcgattatg gaaattagat taccaaaatt attagcagga attatagctg
     2281 gctggtcttt tggattggca ggatttatct ttcaaactat gttaagaaat cctcttgcaa
     2341 gtcctgatat aatcggtgtc acaagttctt catctattgc agcggtcttt tgcatattgg
     2401 tattaaaaac aaatagttta actactggaa ttatttcaat aacttgtgga ctaacatcat
     2461 ctttaatatt atttttacta gctaaaaaag atggtttttc agcagcaaga ctgataatat
     2521 taggcattgg ttttcaagct gtcacaagag caggcacctc atttttattg ttgaaagtag
     2581 caagatatga attacaagaa gttatgagat ggctcagtgg ctctttatct tttacaaagt
     2641 tagatgacat acctcttgtt ctaatagtaa gtattattgc tactatatta gttttatttt
     2701 ttaataaaag actagaaatt attgaacttg gtgaagaaat agcaatcgga cttggagcaa
     2761 atcccgagct ttcaaggctt gttttaattt tttgcgctgt atctttaact gctttttcta
     2821 cttcaattac aggaccaata gcttgtatat cttttttagc tggtcccata gccttaaata
     2881 ttggcaagaa aagaagccca atattagctg gattggttgg aattttacta gttttgttat
     2941 cagacatatt ctctcaaaat attttaccag ctagatatcc agtaggtgtt gtaactggct
     3001 tgttaggttc accatactta atatatttac taa
//
LOCUS       DAVKQB010000100         3008 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKQB010000100 DAVKQB010000000
VERSION     DAVKQB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.100"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..257)
                     /locus_tag="VW528_002092"
     CDS             complement(<1..257)
                     /locus_tag="VW528_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9912842.1"
                     /translation="MENFGAVLKDIRISKNFRLKDLSCNEISESTISRFENGITKLSI
                     NHFYILLNRLGISFSEFEELVHCYYSKKECLFEELEHAVNSS"
     gene            351..1571
                     /locus_tag="VW528_002093"
     CDS             351..1571
                     /locus_tag="VW528_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEU9912843.1"
                     /translation="MKKILLSTVALLSLVASLLANNPVSAQESSSQATYSKSSGSWIK
                     SGNRWWYKHSDGSYTTNGWEKINGTWYYFDSEGWMKTGWIKEYGKWYYLDDSGAMKTG
                     WCLVSGSWYYLNSSGVMQTGLQTINGKQYYLAAGGAMQTGWHNIGDDTYFFANSGENQ
                     NINRRALVLGETSTRAVPIADVNAMEKVFNNQNFSEVVRFPDRTKSEIIAKMQELFES
                     SSEGDVNYLYFTCHGGRDGRIYIGSDGLAFSGWELASVLKQYKGKFVVMLDCCHAGTI
                     ISKDNTGEGNEGASTEYFDLDEFVSGFSNMDGNEKSGEMIDSKFLVLCSSRGAEYSSG
                     GSLSLATKYWSLGSGWNPLQNSQAYLAADQNNNRRITLNELYTYSREQVLKQNSNQHI
                     EVYPDNSQFVLFKK"
     gene            1584..2765
                     /locus_tag="VW528_002094"
     CDS             1584..2765
                     /locus_tag="VW528_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9912844.1"
                     /translation="MKVSKKITLFSLSFAGFVLLTLPQAGKAFELKEDWAFKGGIRYE
                     NGKVSKINNGYEVNIKVLDLPSTSAIEWTVRLNGEKQNTNFLAEERTVSKTEDKGRFL
                     HFYIPYGYRGDIVVEAKSGNEVKTWSTKVVDDVYSDSAKSGYFILDGEQILESSWDSV
                     NESYIATLPTVTSGKTVVAWREKGTLNLIKPGRIARQYNSSGSYVELSPIFETASWLK
                     SNQNWYYQKQGQLVQNSWIKDQGSWYFMDDEGVMFNQTWLHQGGSWYAFKSSGAMISA
                     DWLYDNGSWYYLKDSGSMVTGWLKNGGSWYYLNKSGSMATGWIKDSGTWYYLKNSGSM
                     ATGWVKDSGSWYYLKNSGSMATGWVKDNGKWYYLASSGNMLRNTRTPDGYYVDGSGAW
                     K"
BASE COUNT         1029 a          404 c          629 g          946 t
ORIGIN      
        1 gaagaattta cagcatgttc taattcttca aaaagacatt cttttttgga ataataacaa
       61 tgaactaatt cttcaaattc tgaaaatgat attccaagac ggtttaacag aatataaaaa
      121 tgattaatag ataattttgt aatgccattt tcgaaacgag aaatagttga ttcactaatt
      181 tcattacatg ataaatcttt aaggcgaaaa ttttttgaaa tacgaatatc ctttaaaact
      241 gctccgaaat tttccataat gttcctcttc catttctgca aaaaataaaa cgcttgtttt
      301 ttatttatga tatgattata caaaaataaa atagaaagga caataatatc atgaaaaaaa
      361 ttttattgtc aacagttgct ttattaagct tagtagcatc tctgctagca aataatccag
      421 tttctgctca agaatcatcc tctcaagcaa cttatagtaa gtcaagtgga agttggataa
      481 aatctggtaa ccgttggtgg tataagcatt ctgatggttc ttatactaca aatggatggg
      541 aaaaaataaa tggtacctgg tactattttg atagtgaagg ttggatgaag acaggttgga
      601 ttaaagaata tgggaaatgg tattacctag atgatagtgg tgctatgaaa actggatggt
      661 gtttggtttc tggtagctgg tattatttaa atagtagcgg tgtaatgcaa acgggactac
      721 agactatcaa tggaaaacaa tattatttag ctgccggcgg tgctatgcag acaggttggc
      781 acaatatcgg agatgatacc tatttctttg ctaatagtgg ggaaaatcaa aatatcaatc
      841 gtcgtgcttt agttcttggt gaaacatcaa cgagagcagt acctattgca gatgttaatg
      901 ctatggaaaa ggtttttaat aaccaaaact ttagtgaagt tgttcgtttc ccagatagaa
      961 caaaatccga gataattgca aaaatgcaag agctttttga atcttctagc gaaggcgatg
     1021 tgaactatct ttactttact tgtcatggag gaagagatgg aagaatctat attggtagtg
     1081 atggcctagc attttctggt tgggaattgg cgtccgtttt gaaacaatac aaaggtaagt
     1141 ttgttgttat gctggattgt tgtcatgctg gaacaattat ttctaaagat aatacaggtg
     1201 aaggcaatga aggagcctct acggaatatt ttgatttaga tgaatttgta tcaggatttt
     1261 ctaatatgga tggtaacgaa aaatcaggtg agatgattga ctctaaattt ttagtattat
     1321 gctcatcaag aggtgcagaa tactctagtg gtggatcatt aagtttagcg actaaatatt
     1381 ggtctttagg ttcaggatgg aatcctctac agaattcaca agcatattta gcagcagacc
     1441 aaaataataa tcgcagaatc acattaaatg aattgtatac atactctcgt gaacaagttt
     1501 tgaaacaaaa ttctaaccag catattgaag tttatcctga caacagtcaa tttgtgttat
     1561 ttaagaaata agggggcaat aaaatgaaag tatcaaaaaa aattacacta tttagtttgt
     1621 cttttgcagg ttttgtttta ttgactttac ctcaagcagg aaaggctttt gaacttaaag
     1681 aagactgggc atttaaaggt ggcattcgat acgagaatgg gaaagtcagc aaaattaata
     1741 atggatatga agtaaatatt aaagtgttag atttacctag tactagcgca atcgaatgga
     1801 cagttagatt gaatggagaa aagcaaaata ctaacttctt agcggaggaa agaactgtat
     1861 ctaaaactga agataaggga cgtttcttgc acttttatat cccctatgga tatcgtgggg
     1921 atattgtagt agaggctaag agtggaaacg aagtgaagac ttggtctact aaggtagttg
     1981 acgatgttta ttcagattct gctaagagtg gctactttat tctcgatggg gaacaaatct
     2041 tagaaagttc atgggattcc gtaaatgagt cttatattgc aacgcttcca actgtaacat
     2101 caggaaaaac tgttgttgct tggcgtgaaa aaggaactct taatttaatt aaaccaggaa
     2161 gaattgctcg gcaatataat agcagtggat catatgtaga actttctcca atctttgaaa
     2221 ctgctagttg gctaaaatca aaccaaaact ggtactatca aaaacaaggt caattagttc
     2281 aaaattcttg gatcaaggat cagggaagct ggtactttat ggatgatgaa ggggttatgt
     2341 ttaatcaaac atggctacac caaggtggaa gctggtatgc ctttaagtca tcaggagcca
     2401 tgatttcagc tgattggttg tatgataatg gttcttggta ttacctaaag gactcaggtt
     2461 ctatggtaac aggatggctt aaaaatggag gctcttggta ttacctaaac aagtcaggtt
     2521 caatggctac aggttggatt aaagatagtg gaacttggta ttatctaaag aattcaggct
     2581 ctatggcgac aggctgggtg aaagatagtg gctcttggta ttatctaaag aactcaggtt
     2641 caatggcaac aggttgggtc aaagataatg gtaagtggta ctatctcgca tcatctggta
     2701 atatgcttcg caatacacgc acaccagatg gctattatgt agatggcagt ggcgcttgga
     2761 aataaatttt taaaagagaa aatctactga gaaggtagat tttcttttta gttaatgaat
     2821 atagaataaa gtagatcagg actggtctat tttattttgt aactattaat gaatagtctt
     2881 aatgatgaag ttactttctg aagatattta attgtgttga gatttgataa aactatagcg
     2941 gattgaaata agatataaac aaagagatta tggaagtcaa attaatttct aaaaacgttt
     3001 taaaatcc
//
LOCUS       DAVKQB010000101         2830 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKQB010000101 DAVKQB010000000
VERSION     DAVKQB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.101"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..143)
                     /locus_tag="VW528_002095"
     CDS             complement(<1..143)
                     /locus_tag="VW528_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9912845.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     GKEA"
     gene            348..536
                     /locus_tag="VW528_002096"
     CDS             348..536
                     /locus_tag="VW528_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9912846.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            693..1058
                     /locus_tag="VW528_002097"
     CDS             693..1058
                     /locus_tag="VW528_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9912847.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            1061..2728
                     /locus_tag="VW528_002098"
     CDS             1061..2728
                     /locus_tag="VW528_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9912848.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT          901 a          516 c          624 g          789 t
ORIGIN      
        1 gcttcttttc ctttctgttg ataggctagt agcagacgat tgatttgtct ttcagaaaga
       61 ttgagttcga cacaggcccg tttctttgtt ttctttcctt gggctatagc ttttatcaca
      121 agatattttt tcgtttcatt catattcagt tggatccttt tcatatgact attctaccaa
      181 atgtgacatt ttcacgttcg atttactaaa gacattatca cattcgaatc acaacacaaa
      241 taaaaaatct tccaattccc ttgcaaaaaa caagaaagtg tgataacata gtacggtatg
      301 tggtgctagc acatccgcta tattagatct aataggagga aaacacaatg gctaaagtat
      361 gttactttac aggtcgtaag actgtatcag gaaacaaccg ttcacacgcg atgaaccaaa
      421 caaaacgtgc cgtaaaacca aaccttcaaa aagttactgt tcttatcgat ggtaaaccta
      481 aaaaagtttg ggcttcagct cgtgctttga aatcaggtaa agttgaacgc gtttaataat
      541 aaaagcaaag gagaccttag ggtctttttt cttttattgt gggtataaca tcgtttgaaa
      601 aatagagtaa atatccgctg atacatcatt ctgcttttac acttgggctg aaatatgata
      661 aaatagagta tcaactagtt gaggtaaaaa ggatgactgt aaaaattaat acaaaagatg
      721 gtcaaatcga actaacagat gaagtgattg caaccgtcgt aggtggagca gcaactgaga
      781 tttttggtgt ggtcggtatg gctagtaaaa atgccctcaa agataatttc caagccctgc
      841 taggtaagga aaattattct aaaggtgtcg tcgtaaaggc agccgaagat ggcagtattg
      901 cagttgatgt atataccgtg ttaagctacg gaacaaagat tagtgaagtg tcaaaaaaca
      961 ttcaagagcg tgttgccttt agcttggaaa accagcttgg aattactgct cagactgtaa
     1021 atgtctacat tcaaaatatc aaagttgtag gagaataacc gtgtcaaaga ttactactag
     1081 cttatttcaa gaaatggtgc aggctgcatc aactcgcttg aataagcaag ctgaatatgt
     1141 caattcatta aacgtctttc cagttccaga tggagatact gggacaaata tgggaatgac
     1201 cattgaaaat ggtgctaaag aagttgcaga caagccagct tctacagttg gagaagtagc
     1261 gagcattctt gccaaagggc ttttgatggg tgcgcgtggg aactcaggag tgattacatc
     1321 tcagcttttc cgtggatttt cccaagctat caaggataaa gacgagttaa caggtcaaga
     1381 cttggctctg gccttccaat caggtgtgga agttgcctat aaggcagtga tgaaacctgt
     1441 tgaaggaacg attttgacag tttctcgtgg agctgctatc ggtgctaaga aaaaagctga
     1501 gcaaacagat gatactgttg aagtcatgcg cgcagccttg gaaggtgcta aaacagctct
     1561 agctaaaaca ccagacatgc ttccagtatt gaaagaagtt ggcgttgtgg actcaggtgg
     1621 tcaaggattg gtcttcatct acgaaggttt cctttcagcc cttactggcg aatatattgc
     1681 atctgaggac tttgtagcga ctcctgccaa catgagtgag atgatcaatg tagagcatca
     1741 taagtctgta gctggtcacg tagcgactga ggacatcacg tttggttact gtactgaaat
     1801 catggtagct cttaagcaag gtccaaccta tgctaaagat tttgactacg atgaattccg
     1861 taactacttg gatgagcttg gggattctct cctcgttgtt aacgatgatg aaattgtcaa
     1921 agtccatgtt catacagaag atccaggact tgttatgcaa gaaggtctca aatatggtag
     1981 cttggtcaag gtaaaagttg acaatatgcg taaccaacac gaagcacagg ttgagaaaga
     2041 agctactcaa gttatcaagt cggctgaaga aaaagagtat gctttgattg ctgtggtggc
     2101 tggtaaaggt ctagcagata tcttctgttc tcaaggcgtg gattatgtta tcgaaggcgg
     2161 tcaaaccatg aacccttcaa cagaagactt tatcaaggct gttgaacagg tcaatgcccg
     2221 taacatcatc ttcttgccaa acaacaagaa catcttcatg gcagctcaat ctgcggcaga
     2281 agttttggag caaccagcag tagtggtaga ggctcgcact cttcctcaag gtatgacaag
     2341 tcttcttgcc tttgatccaa gcaagtccat tgaagaaaac caagagcgta tgacagctgc
     2401 tcttagcgat gtcgttagcg gaagcgtcac aacagccgtg cgtgatacaa cgatcgatgg
     2461 cttagaaatc catgaaaacg ataatctagg tatggtggat ggaaaaattc ttgtgtcaaa
     2521 ccctgatatg caccaaacat tgactgaaac cttgaaacat atgttggatg aagacagtga
     2581 aatcgtaacc ttctatgtcg gtgaagacgg aagcgaagaa cttgccaatg aaatcgctca
     2641 agaaatcgta gaagaattcg aagacgttga agtcgagatt caccaaggtc aacaacctgt
     2701 ttacccatac ctatttagtg tggaataaga ttaatgaagg actgagaaat cagttctttt
     2761 ttctttttta gtaaatgaaa tcggtatctt tttaataaaa acaaaaataa cattcataaa
     2821 taaacgttaa
//
LOCUS       DAVKQB010000102         2813 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKQB010000102 DAVKQB010000000
VERSION     DAVKQB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.102"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..1002)
                     /locus_tag="VW528_002099"
     CDS             complement(<1..1002)
                     /locus_tag="VW528_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912849.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTSQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     "
     gene            complement(1012..1965)
                     /locus_tag="VW528_002100"
     CDS             complement(1012..1965)
                     /locus_tag="VW528_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9912850.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIID
                     DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDE
                     YMPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIA
                     GTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENG
                     ITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKV
                     IKKAREEKIFNEFLFLKYMLLYYFGFFYCIYDNYVQLKFRK"
     gene            complement(1958..2813)
                     /locus_tag="VW528_002101"
                     /pseudo
     CDS             complement(1958..2813)
                     /locus_tag="VW528_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          967 a          524 c          297 g         1025 t
ORIGIN      
        1 gcaaatattt tttgcttcat ataacggaaa atctctattc acctcatttg gttcgatatc
       61 attcatttta tttgactcaa ttggattaat tgaatacaat tttaaaaaaa taggcttcgt
      121 accaataata gaggataatt gacctcctaa ttcacctagc gctgtaaaaa taacacctgt
      181 tagtgctcct attgcttcaa tagtaccaat tttcactatt ccttttattg caagatagcc
      241 tgttaaaaaa acgagagata tctgaaaaaa aatattgaga aagaagctaa tagcgcctgc
      301 taacgtttct acagttgtct ttctttgtat aaccatcttt aataaaattc ctgcttcttt
      361 aattttctta ggcaatacat ataaaagatt caaggacgct aacacatcaa atccattcaa
      421 tatagtctca ctagatttta aaaaagcttc attttggcta gttaaattta gactaacttc
      481 tcgcattttc gatgcaaaga tttttggtac aagtagcata atcattaatg aaaacaaggt
      541 ggctacagtc aatgaccaat gatagtgatt aagagtcaca actgcaaata tagtaccaga
      601 aattcctttt attactaaaa aaagttgttt aaaagcctga tcatttaaag tctgaacatc
      661 attatttagc cacgaaagat atgttcctga tgatttacta tgaaattctt gataggtaga
      721 gttagagatg tctgtggcaa ctctatttcg aatctctaga ttaaactctt ggatcacttc
      781 aacctgataa tttttcacta cccagtcaag gaatattatc ccacaccaga caatcatttg
      841 gtagattgac aatttcaaaa accgctctaa attcatcgca attaattcat tcaacaccag
      901 agcattaata gttgctgcat aaattagcaa taattgacca gcaacaataa atatcgttaa
      961 taaactaaat ttttttatat ttgattttat aatagtatac acaatagttt ctcactttct
     1021 aaattttaat tgaacatagt tgtcatatat acaatagaaa aaaccaaaat aatataataa
     1081 catgtatttc aaaaaaagaa attcgttaaa aattttttct tctcttgcct tcttgattac
     1141 ttttaaagcc ttgcatttgt ctcctattaa tagtaaccgc tttatgttta aagaataata
     1201 tttctttgta accaatattc tctcgttgaa actcaataaa ttaaaatatt tcctacagta
     1261 attataatat tcttcatctg cattaattgt tttttgtgtc actccagtga taccgttttc
     1321 tttactgtga gcgtagtaat tcaccaagaa ttctcgcact atatcaattt ggtatccttg
     1381 agcaagtagt tttaataaaa caacaccgtc ctgatgtgaa tctattttct caaaaccatt
     1441 aattaattct agcacctctt ttttacacaa ccaaaatgac gtacctgcta tattgtgaac
     1501 catttgaaca aacaagggat ttccaacaaa atcggtcttc tcctcttctc gtgtaccatt
     1561 tggataaatt attattccat aactataaac taaagctaaa ttcttcattc tactcttttt
     1621 aaaacaagcc atcaacttta aaattcgatc tggcatatat tcatcatcat cgtctaaaaa
     1681 tgatatatac ttacctctag aattttttat acctatgttt ctggcattag ttgcacctaa
     1741 atcttcatta cttaaaatta acttaattct atgatttgta tagccaaatt gatggataat
     1801 tttatttctt aaatttacat tactataatt atcatcaata attataactt cgatattttt
     1861 ataactttga tgtaaacaac ttttcacagc tctaatcaga gattcatacc tattatgtgt
     1921 tggtattata atacttacta attcttgatc tatattccta tccatgacta ctcttctcta
     1981 ataattcatc atatactctc atggtttcta caaacatttt ttgcacagaa aaatgttttc
     2041 ttatttttga tttactattc tcacctatat atttcaaata ctcagaatca ttgagtaaaa
     2101 aattagcaca agcacacact ccctcaacat cttccttctc aaataaaaat ccatcaaccc
     2161 tatgttcaat aatttcactt aacccgccaa cattactagc taaaaccgga gttccttgtg
     2221 acattgactc taaaacacac ataggtattc cttctgtatc agaaggaata tacaataaat
     2281 ccgatatttg gtaaactata gtagctggat agatttcacc aagtaacctg aaattatctc
     2341 tacatttcaa atggcaaatt ttttctttca aagcagccca catactacca tttccagcca
     2401 taataaaaat cacatcttct ctgactaaaa ataatttttc tgcaaattca aggaatctat
     2461 ccggcctttt ttctggatcc aaccttccaa tataacaaat gatttttttg ttatttggaa
     2521 tacaaaattc ttttttaaag tcttgaacac ctactacatc taaatcgcta tttgatacat
     2581 taattccgtt atttattgca attatcttct tattttttat tatactctcc aatctttttt
     2641 catagtttca gatacacaaa taaaagcatc tcccatagaa tatgtccaaa aatcaaaata
     2701 agtcaagaat ttctttttta agttatattc aacccatcca tggcatgtta tcactgtctt
     2761 aacctttcca aatccattct tgtcaagttt ttttaacata tataaaaaat aat
//
LOCUS       DAVKQB010000103         2574 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKQB010000103 DAVKQB010000000
VERSION     DAVKQB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.103"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..429)
                     /locus_tag="VW528_002102"
     CDS             complement(<1..429)
                     /locus_tag="VW528_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912851.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCMGDFPHDFFDIYLDHVAKYAYEQKVNNIKEYYPLK"
     gene            complement(443..1156)
                     /locus_tag="VW528_002103"
     CDS             complement(443..1156)
                     /locus_tag="VW528_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9912852.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1616..1996)
                     /locus_tag="VW528_002104"
     CDS             complement(1616..1996)
                     /locus_tag="VW528_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU9912853.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQTQLAELEINK"
     gene            complement(2413..2565)
                     /locus_tag="VW528_002105"
     CDS             complement(2413..2565)
                     /locus_tag="VW528_002105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912854.1"
                     /translation="MLEKVTDSSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDSLLA
                     LLKESK"
BASE COUNT          836 a          501 c          392 g          845 t
ORIGIN      
        1 ttttaaagga taatactctt taatattatt aactttttgt tcgtaggcat attttgctac
       61 atgatctagg tatatatcaa aaaagtcatg aggaaaatct cccatacagc cattacgatt
      121 catattgagg ctatattttc gattagtaag ataattcgga aatatatttc ctattactct
      181 aagattatct acttgtgaat cccttctccc aatataatcc ccttctttga ctaatgatgg
      241 agatttccaa gtaaaaatta aatcgccaga gaaatttttc ttatcaaaaa acttaaaaag
      301 agattgataa atactatttg ctaattgaca agtgtcagat gtaaattcat aattaacagc
      361 ttcattaaaa tcaacttcag acatttcaat gtactcttca atagtaatat tagctttttt
      421 tgtagtcatt agctaatctc ctttaaaatg tgatcttttc aagaatattt ttatctaaac
      481 aatccagcaa gtcttggtaa gaatagactt cgtaagtcgg ctgggcttgt gtgtgatttt
      541 cgaggtgatg aggattatac cagatagtgt caatccccgc attattgcca ccttgaatgt
      601 cggcggttag agaatctcca atcatcagcg tcttttcttt actaaatcca gcaatttgct
      661 ggccaatctt ttcataaaag agagcatccg gcttttgagt ttgcaactgt tctgagataa
      721 agacttgatt gaaataaggt gctagaccag attgagccaa acgtcctgtc tgaatggcag
      781 taatgccatt tgtcgcagca tacaagtcat aatcacgctc aatgaggctg tccaagagat
      841 catgagcgcc cgatagtgtt tgtccctgct gggcgaggta aaattggtaa cgctgggcaa
      901 gaaaactacc gtctttttcc tgtccaaaat gagaaaataa acgagaaaag cgcgtgttaa
      961 ccagctcttg tttactgatt ttcttcagct ccaagtcttt ccagagagcc ttgttcatag
     1021 gaacgtaata atctttataa gcctgaatat ccgcaactcc ttcttctttt agaagttggg
     1081 tcaaagccac atcctcagca gcatcaaaat caagaagagt gtggtcgagg tcgaagagta
     1141 gaaatttgta gaacaatttg aggttttcct ttctgaaaat tcattaaaaa cattatatca
     1201 taaagcacct catacaatta actaatttaa tcacttaaaa aaaattcgaa cactttctat
     1261 acaactgaca gctcaaatct ttcagaatag aacaatacta actatcgagc acccccgtct
     1321 tcataaatat atgtgtaatt ctagacctag aattcctata aactaaatgc tttcatactc
     1381 ttccaagtaa ttgattgcct taaattttaa tttttgaagg attctaaagc tagaatagcc
     1441 ccatcacaat cagttttgat tgattcacaa tttagaaaca ctatagtttc actcctgtta
     1501 aaataaaaag gaactgcata aagcaatccc tttctaattt tgaaatcatt tacttaacat
     1561 tttatagttg agataatcaa tagcttacct ataaaaagag ttatagtaaa attccttatt
     1621 tattgatttc aagctccgct aactgtgttt gaataactga cagttctgca ccagcctgca
     1681 gaagagcagc tgcattatag gcaccttcta caattggaac cctgttgatg atgatacttt
     1741 tatcactgaa atcagtcacc atttttaagt tcattttagc agaacctagg tcaaaaaagg
     1801 caagtaaagt atctgctgga ttttcggaaa caaccctatc tacttgatca aaactcgttc
     1861 caattcctcc gccctcggtt cctcctacat aagtaatcgg aacatcttta gctactttac
     1921 taatcagttc aacaacacct tctgcaatgt gtttggaatg tgaaacgata acaagaccaa
     1981 taccaatact ttccatcaaa ccaccccagt ttctaaaata gcagtaaaga gtaatcctga
     2041 tgagaatgat ccaggatcaa tatgtccaag aaaccatatg ctcctaagac aagagccaac
     2101 agactggtca tcaataatag tattgttctt tttttcatca ttactcctta actagtgttt
     2161 aactgattaa ttagccagta aatagtttat ctttatttac actatctgtt aagatatagt
     2221 aaaataagaa caggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtatact
     2281 attctagttt caatctacta tattttgcac attttcatga aaaaaatgag aactagaact
     2341 cacattctgc tctcattttt cgttttcccg ttctcctatc ctgtttttag gagttagaaa
     2401 atgctgctac ctttacttac tctcctttaa taaagccaag agactatcta cttctgctga
     2461 aatagtgtta ttatctttcg ttccgagaag aagactactt tttagaccag tcaatgtctc
     2521 cacagcattt tgtctaatac tagaatctgt tactttttct agcaaggcct ccgc
//
LOCUS       DAVKQB010000104         2484 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKQB010000104 DAVKQB010000000
VERSION     DAVKQB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.104"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..686)
                     /locus_tag="VW528_002106"
     CDS             complement(<1..686)
                     /locus_tag="VW528_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU9912855.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHSTEGSHLKKLKSYYL
                     GEHAILEHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMAELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGD"
     gene            complement(698..1996)
                     /locus_tag="VW528_002107"
     CDS             complement(698..1996)
                     /locus_tag="VW528_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEU9912856.1"
                     /translation="MSQTYQIKQNDIVVDLPKTVGAGYGQFWRSRNLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDVGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETSRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFDKDELLRDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAESRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYTIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            complement(1974..2453)
                     /locus_tag="VW528_002108"
     CDS             complement(1974..2453)
                     /locus_tag="VW528_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEU9912857.1"
                     /translation="MAFFDYSKGGDGKLSGLRIKQKRFADEYIISGNATEAYKKAGYR
                     VSSDRVAGVEGHKLLKNPKIKSYIDERLKQLDSEKIADQQEVLSYLTSVMRGETQEQT
                     LISIGELGQTITDIDVGAKDRIKAAELLGKRHRLWTDKVEADVSGTVVFANESDIPD"
BASE COUNT          660 a          595 c          433 g          796 t
ORIGIN      
        1 tagtcaccaa ttgcaaagtg attgttgcga tattcgataa ttgggatttg accaaggttg
       61 tgaggtgttg cctcctcgct ctgagttgtt cctgaatctg tacttctcag caccatgtga
      121 tagtgcagat tttcggtaaa gacctcagcc tggtgcttgg tagtgtcttt cgtatcgtct
      181 tttacttcat agtaatagac cgcaaacaaa ggcttccgct caatactatc atcgtagacc
      241 atgaaagtat tctccggatc aatactagtt gaatccaact cagccatacc ctctttagca
      301 tagatgtact cgtaagcacg accatagata gccatgttca aagcattctg agcatctact
      361 tggtcaatct cagcaccatc aaaggctgta agtagttcat cgatatcacc gtcagcagta
      421 ttgttatact tgataggatt gcccataaaa tagcccgtag ccgtgtctgc gatatccttg
      481 gcatgattgg ctaccgtctt gtaattaggt gcgttcacgt tgcgtctcgt gtgttctaag
      541 atagcatgct cacccaaata gtagctttta agcttcttca aatgtgagcc ttcagtgcta
      601 tgtatcgtta tcaatttgta aatcaggtct ttcttcaaag aaccctcatc atatccatcc
      661 cgtggatagg ttaaatattg gtacatgtct ttcctctcta tagaccataa tcagaacgtc
      721 tgcggacggt tgctttccca ccttcgatac attgaaggct gtaacgcaaa gcgtccatca
      781 agtggttgtt tttatcctct ggtttattca accagttgcc ttctttatct cgctggtagc
      841 agtaactata aaattcatcc atgatgtttt tacaatctgg atgcacataa atagtgtatc
      901 cttgcaattt tgatacgcct gccataatac tatccttacc tttccgactc tcttttattc
      961 tagatatgcc atgttctgac ctgagctctt caatcagccg tgactctgcg ctatcagcaa
     1021 tgatttgtga gcgatgataa cctttgtctt ttatcatctt cgcaacttct ttggttatca
     1081 atccgacttt atacgcctca tcaaagacat aaatctcttt cgtcgtgtca tttatcaacg
     1141 aacaacacaa agcggttgga tcgtgagtaa aaccaaagtc aagaccgata cataacttat
     1201 tagctgaatc tcgtagcaat tcatccttat cgaaatcctt gacggtcacg ttctcataga
     1261 ttaaaccttc agcaactccc cattcaccat cacaaacgat tctcgcacgc cttggatttg
     1321 tgtgatacaa gtcctcatag cgcttaatat cgacttcatc aagccactca ttgcatttgt
     1381 aagtggttgt agtagcgaat gtatcagctc ggctagtctc ttcatcaaag aacacacgtt
     1441 tgagccagtg cctctcattc cacgggttaa atgtgactgt gatttgttta aagaaatcag
     1501 gtacatctaa gctaccacgg attgactcaa ctactgtact gaacttgtct tcagtctcaa
     1561 tttgatatgc ttcctcaaac catgcccaac aaagactgcc gacatcgacc gtgatagatg
     1621 tgattttgag ttcatcatcc aaaccacgga ataggatttt ttgaccagtc gcttttatgg
     1681 ttatttcagg caaagattca ttgaatttaa acaaatgagt cacacccaac acattacacg
     1741 cccatttaaa atccgtataa gttgattgct tgttggtatt cgagtatcta cgaataacaa
     1801 gcaagttggc ccagggatac ttcaaaagac ggataacata attcaaagcg gttgtcttgg
     1861 acttcttcga accacgggaa ccttttacaa cacgataaag atttcttgag cgccagaact
     1921 gtccgtatcc agctcctact gtcttaggta agtcaacaac aatatcgttc tgtttaatct
     1981 ggtatgtctg actcatttgc aaacaccacc gtcccagaaa cgtctgcctc tactttgtct
     2041 gtccaaagcc tatgacgttt tcctaatagt tcggctgcct tgattctgtc ttttgctcca
     2101 acatcaatat ccgtaatcgt ttgacccaat tctccgatgc ttatcaaagt ctgttcttgc
     2161 gtctctcctc gcattaccga ggttagataa ctaaggactt cttgctggtc tgcgattttt
     2221 tcagaatcaa gctgtttcaa ccgttcatct atataacttt taatcttagg attctttagt
     2281 aacttatgtc cttcaacgcc tgccactcta tcactagaaa cacgataacc tgctttctta
     2341 taggcttccg tcgcattacc tgagatgatg tactcatctg caaatctctt ttgttttatt
     2401 ctcaatccac tcaattttcc atcaccaccc ttcgaataat caaaaaaagc cacacgatgt
     2461 gcgaccttct tgcaaggcga ctac
//
LOCUS       DAVKQB010000105         2484 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKQB010000105 DAVKQB010000000
VERSION     DAVKQB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.105"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..686)
                     /locus_tag="VW528_002109"
     CDS             complement(<1..686)
                     /locus_tag="VW528_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU9912858.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHNTESSRLKNLKSYYM
                     GDHAILNHKRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMTELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGD"
     gene            complement(698..1996)
                     /locus_tag="VW528_002110"
     CDS             complement(698..1996)
                     /locus_tag="VW528_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEU9912859.1"
                     /translation="MSQTYQIKQNDIVVDLPKTVGAGYGQFWRSRSLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDIGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFNKDELLQDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAESRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            complement(1974..2453)
                     /locus_tag="VW528_002111"
     CDS             complement(1974..2453)
                     /locus_tag="VW528_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEU9912860.1"
                     /translation="MAFFDYSKGGDGKLSGLRIKQKRFADEYIISGNATEAYKKAGYR
                     VSSDRVAGVEGHKLLKNPKIKSYIDERLKQLDSEKIADQQEVLGYLTSVMRGETQEQT
                     LISIGELGQTITDIDVGAKDRIKAAELLGKRHRLWTDKVEADVSGTVVFANESDISD"
BASE COUNT          661 a          596 c          431 g          796 t
ORIGIN      
        1 tagtcgccaa tcgcaaagtg attattgcga tactcgataa ttgggatttg accaaggttg
       61 tgaggtgttg cctcctcgct ctgagttgtt cctgaatctg tacttctcag caccatgtga
      121 tagtgcagat tttcggtaaa gacctcagcc tggtgcttgg tagtgtcttt cgtatcgtct
      181 tttacttcat agtaatagac cgcaaacaaa ggcttccgct caatactatc atcgtagacc
      241 atgaaagtat tctccggatc aatactagtt gaatccaact cagtcatacc ctctttagca
      301 tagatgtact cgtaagcacg gccgtagata gccatattta gtgcgttctg cgcatccact
      361 tggtcaatct cagcaccatc aaaggctgta agtagttcat caatatcacc gtcagcagta
      421 ttgttatact tgataggatt gcccataaaa tagcccgtag ccgtgtctgc gatatccttg
      481 gcatgattag ctaccgtctt gtaattgggt gcgttcacgt tgcgtctctt gtgatttaaa
      541 atagcatgat cacccatgta gtagcttttt aaattcttca agcgtgagct ttcagtgtta
      601 tgtatcgtta tcaatttgta aatcaggtct ttcttcaaag aaccctcatc atatccatcc
      661 cgtggatagg ttaaatattg gtacatgtct ttcctctcta tagaccataa tcagaacgtc
      721 tgcggacggt tgcttttcca ccttcgatac attgaaggct gtaacgcaaa gcgtccatca
      781 agtggttgtt tttatcctct ggtttattca accagttgcc ttctttatct cgctggtagc
      841 agtaactata aaattcatcc atgatgtttt tacaatctgg atgcacataa atagcgtatc
      901 cttgcaattt ggatacgcct gccataatac tatccttacc tttccgactc tcttttattc
      961 tagatatgcc atgttctgac ctgagctctt caatcagccg tgactctgcg ctatcagcaa
     1021 tgatttgtga gcgatgataa cctttgtctt ttatcatctt cgcaacttct ttggttatca
     1081 atccgacttt atacgcctca tcaaagacat aaatctcttt cgtcgtgtca tttatcaacg
     1141 aacaacacaa agcagttgga tcatgagtaa aaccaaagtc aagaccgata cataatttat
     1201 tagctgaatc ttgtagtaat tcatctttat tgaaatcctt gacagtcacg ttttcgtaga
     1261 ttaaaccttc agcaactccc cattcgccat cacaaacgat tctagcacgt ctggggttcg
     1321 tatgatacaa atcctcatag cgtttgatat cgacttcatc aagccactca ttgcatttat
     1381 aagtggttgt agtagcgaat gtgtcagccc gtctcgtctc ttcatcgaag aatacacgct
     1441 tgagccaatg cctctcattc cacgggttaa atgtgactgt gatttgttta aagaaatcag
     1501 gtacatctaa gctaccacgg atagactcaa caaccgtgct gaacttgtct tcagtctcaa
     1561 tttgatatgc ttcctcgaac catgcccaac acagactacc gatatcgact gtaatagatg
     1621 taattttgag ttcatcatcc aaaccacgga ataggatttt ttgaccagtc gcttttatgg
     1681 ttatttcagg caaagactcg ttaaatttaa acaaatgagt cacacccaac acattacacg
     1741 cccatttaaa atccgtataa gttgattgct tgttggtatt cgagtatcta cgaataacaa
     1801 gcaagttggc ccagggatac ttcaaaagac ggataacata attcaaagcg gttgtcttgg
     1861 acttcttcga accacgggac cctttgacta cacgataaag acttcttgag cgccagaact
     1921 gtccgtatcc agctcctact gtcttaggta agtcaacgac aatatcgttt tgtttaatct
     1981 gatatgtctg actcatttgc aaacaccacc gttccagaaa catccgcctc taccttatcc
     2041 gtccaaagcc tatgccgttt accaagaagt tcagccgcct tgattctatc tttagctcct
     2101 acatctatat ccgtaatcgt ctgccctagt tctccaatgc ttatcaaggt ctgttcttgc
     2161 gtctctcctc gcatgactga agttagataa cctaagacct cttgctgatc cgcaatcttt
     2221 tcagaatcaa gctgtttcag tcgttcatct atatagcttt taatcttagg attctttagt
     2281 aacttatgtc cttcaacgcc tgccactcta tcactagaaa cacgataacc tgctttctta
     2341 taggcttccg tcgcattacc tgagatgatg tactcatctg caaatctctt ttgttttatt
     2401 ctcaatccac tcaattttcc atcaccaccc ttcgaataat caaaaaaagc cacacgatgt
     2461 gcgaccttct tgcaaggcga ctac
//
LOCUS       DAVKQB010000106         2419 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKQB010000106 DAVKQB010000000
VERSION     DAVKQB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.106"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(97..669)
                     /locus_tag="VW528_002112"
     CDS             complement(97..669)
                     /locus_tag="VW528_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9912861.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(650..1621)
                     /gene="ruvB"
                     /locus_tag="VW528_002113"
     CDS             complement(650..1621)
                     /gene="ruvB"
                     /locus_tag="VW528_002113"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9912862.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1782..1874
                     /locus_tag="VW528_002114"
     CDS             1782..1874
                     /locus_tag="VW528_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9912863.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT          748 a          551 c          425 g          695 t
ORIGIN      
        1 ttttataggg agattattat gaaaaagttt taggagttta agttaaggtc ttcttaactt
       61 atgaacttag tgtacactcc ctagcttaaa gtttccttaa gtatttttaa aaatcaaatt
      121 tttccatttc tcctgccaat ttttcttgga taaacgtgtt tgatagagtt ccattcggtc
      181 ttcattttct aagaaatgag gagttggacg aacttgaaaa ttcaaaatat cctccaaacc
      241 ataaggtgca tagagttcaa aatctgattc ttcattcaag cgcagtccaa ctgtcgtaca
      301 ccgttctgga tacttactca tagcatcacg agaactggta taggaagcag tgtgaggact
      361 gtgctgatgc atatagacct gatttttcaa ttcccactgg tactgaggaa aatcctctcg
      421 cagctttttc tccagtaata aggtttcctc ataagaaaaa tctggatcaa agaaaatcac
      481 atctatatct gtttcatgat caaaagggga tttgtctgac aaaagattcc aaatgaaatt
      541 tctgacagaa cctgctgcca accacgagtc tttcagacca aggtctcgga tgatcgtcag
      601 aatggccatc atatctggat tttctctaaa agcctctaag atttcttgct tatttttcac
      661 tgtattcata acctaagtgc tcatatgcct tagcagtcgc cacccgtcca gaccgtgtcc
      721 gcatgataaa acctttttga atcaagtaag gctcatacat atcttcaact gtctcacgct
      781 cttcggcgat attcacagaa agagttccta gaccaacagg tcctccactg tacatctcaa
      841 tcatggtacg aaggattttt tgatccacat agtccaaacc ttcatggtca acatccagca
      901 tagtcaaagc cttatcggta ataatatcat caattacccc attccccatt atctgggcaa
      961 aatcgcgcac gcgcttgagg agacgattgg caatacgagg ggtcccacga ctacgtaggg
     1021 ccaactcaga tgctgcctca tgagtgattt ccatctcaaa aatatctgcc gtccgctcga
     1081 caatttctgt caagtcagca tgggcataat actccatatg gcctgtaatc ccaaaacgtg
     1141 cccgtagcgg attggagagc ataccagccc gagtcgtcgc accaatcaag gtaaaaggtg
     1201 gtaactccaa atgaacacta cgactaccct caccagcccc aatcataata tctatgtaga
     1261 agtcctccat agcactataa agcacctctt ccactgacat tggcaaacga tggatctcat
     1321 caataaaaag tacatcccca ggctctaact cattcaaaat agctaccaga tctccggctt
     1381 tttcaatgac tggacccgac gtctgcttaa gattgactcc cagttcgttg gcaataacaa
     1441 aggccatggt cgttttcccg agacctggag gcccaaataa gagcacatga tccagcgctt
     1501 catcccgcat tttggcagct tcaataaaga tttgtagctg gtccttgacc ttatcctgtc
     1561 cgatatattc acgtaaatac tgaggacgga gcgtgcgttc tactaactcc tcatccccca
     1621 ttatctcatt atctaaaatt ctactcatgg ctctattata tcaaaaaacc aagccacaaa
     1681 caaaaaagcc acctgattgg gtgactccta agtttagcac ttatgtggta taatattcta
     1741 cgacactgct acaccgccta cgaaaggagg tgagatagcc catgatggaa ttagtactca
     1801 aaactatcat cggaccaatt gtggtcggtg tcgttcttcg tatagtcgat aaatggctaa
     1861 acaaggacaa atagtgtcaa aaaagacccc aagcttattt ggtcgtgagc ttggggtctt
     1921 ttctagccta tgatatagaa ctagtactca attccttttt attatctcat agttcacgaa
     1981 ttttgtcaaa ggtttacatt ttcttcaacc gctgtacgac aagacggtta agattaagag
     2041 aacgttaggg attctatcaa tttcatagaa attttgattt cgtaaacgaa gagacaatct
     2101 tacatgtcac ttctcattta atacgccact actagacaag caaaatcatt attacagtag
     2161 ttccagttct tcaattaaca gtcacttaca atcaaattga gtttgaacta gctgaagcga
     2221 ccacagacct atttcttagt tatattcgct aaaaaagtcc ccgccaattc cccgaccaaa
     2281 atccgaaaaa taccgaaaaa tatcgaaaaa ttatttttag aatagtcccc aaaagcctga
     2341 aatagagcta aaaaactcca cctgattggg tggagttaag ggagattatt atgaaaaaga
     2401 aaagtttagg attttatta
//
LOCUS       DAVKQB010000107         2133 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKQB010000107 DAVKQB010000000
VERSION     DAVKQB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.107"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            40..491
                     /locus_tag="VW528_002115"
                     /pseudo
     CDS             40..491
                     /locus_tag="VW528_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            656..955
                     /locus_tag="VW528_002116"
     CDS             656..955
                     /locus_tag="VW528_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9912864.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1064..2133)
                     /locus_tag="VW528_002117"
                     /pseudo
     CDS             complement(1064..2133)
                     /locus_tag="VW528_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          660 a          416 c          368 g          689 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagt gaatacagac tatattactc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gaaaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact cctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tcaaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattggcta atactcttcg aaaataaaat tcaaatcacg taagcgtcgc cttaccgtac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgttataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcaactag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataaca aaacgcatag
     1021 tagcaagagt tttgtactga ccccaaaaag ttagacaaat aatttatccg aaggatttag
     1081 ttctgtattg cacaaggcta agtcctttta gttttacctt aattcgttta ttgttgtagt
     1141 aatcaatata gtctacaata gcttgttcca attgattaag tgatttaaat gttttctcat
     1201 agccataaaa catttccgat ttcagaatcc caaagaagga ctccatcata ccgttgtctt
     1261 ggctgtttcc cttacgtgac atggatgctt gaattccctt accctcaaga aactgatgat
     1321 aagaatcgtg ttgatattgc cagccttggt cactatggag aatcgtattc tcgtagtgct
     1381 tctctgtgaa tgcctgttcc aacattgttt gtacttgttc taagttgggt gaagttgaaa
     1441 gattataggc gataatttcg ctattaaagc catctaaaac tggtgataag taaagctttt
     1501 gagtacttgc tggaatggca aattctgtca catctgtata gcactttccc attgttttag
     1561 agccttcaaa ttggccttga atgagattct ctgccttctt gccaacgtct cctttatgag
     1621 aagaatattt tcgtttctgt cgcattttag cttgtaaatt gagtactttc atcaagcctt
     1681 gaactctttt atgatttacc agataaccac gatttcttag ttctaaataa atccgacgat
     1741 aagcataatt tcccttgtgt tcgataaaaa tggattgaat ttcagcttta agctcttggt
     1801 ccttatctgt tttgtctagc tgtttcaagt gatagtagta ggtcgaacga gctagtttaa
     1861 tggattttag aagaagaact aacgaaaact cagtcattaa ttcttgaaca atttctgtct
     1921 ttcttctttc tctttttcct ccttcaatcg gagttctctt aactttttta ggatggcatt
     1981 ctccgctctc aggtactctc ccttttgttt tctcaacaat agtatacccg tttttcctgt
     2041 attgtgctag ccagttaaga agtatcgtac gacttgggag gccgtattca agagaaactc
     2101 tatctttagt ccagccttca tgtcagactt tat
//
LOCUS       DAVKQB010000108         2125 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKQB010000108 DAVKQB010000000
VERSION     DAVKQB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.108"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            477..1268
                     /locus_tag="VW528_002118"
     CDS             477..1268
                     /locus_tag="VW528_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912865.1"
                     /translation="MNFALNKGEIVGLVGRNGVGKSMLMKILAQNNQPTSGNIISSDN
                     IGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKNLALHENVGILISSHKL
                     EDIEEICERVLFLESGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEE
                     GLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            1284..2012
                     /locus_tag="VW528_002119"
     CDS             1284..2012
                     /locus_tag="VW528_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9912866.1"
                     /translation="MKLNKLNFLKESIRNLYSSGVIYLSLLISFIPPILLTFFILKTQ
                     GTSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYAWNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTMMMNTSNDLFIKAIELLPFYSLPVFVASNTMSISQ
                     YLVLITTIILLYFFTLKKSKKYSF"
BASE COUNT          729 a          271 c          354 g          771 t
ORIGIN      
        1 tttataagga agatggaacc agcgtcacag ttcgtgctta atgtagttgt ttagttttaa
       61 aaaattatgc aaagctccat atacaacact taaacgagat taaagaatgg cgaaatccct
      121 tgatgcgcaa gagattagct gtcctttttt attttttaag tgataaagtc ggagcttagg
      181 tatcaaagcc tatcaaatta aacaaagaag tgatgtctta gatattttga aaaaaattaa
      241 taagcagaaa actctctatt attttgttgt agagagtttt ttgttaataa aattttacaa
      301 aatgatattt atatattgca ttaagttaga tatatgatat aatgttgtta aaaagaggcg
      361 caacttttta aactcaataa gaatcaaaga gcaaaccatg aatattaatg gaggaataaa
      421 aaatgtaagt tataaatgta agtaagcatt atggtcattc aatcattctc aaagacataa
      481 attttgcact taacaagggt gaaattgttg gtctagtagg gagaaatgga gttggtaaga
      541 gtatgttgat gaaaattctt gctcagaata atcaaccgac ttcaggtaat attataagca
      601 gtgataatat tgggtattta atcgaagaac caaaattatt tttatctaaa acaggtttag
      661 agaatttaaa atatttgtca aatttatatg gtgttgacta caatcaagaa agatttagaa
      721 gtttgatcca agagttagat ttgactcagt ctattaataa aaaagtaaag acctattctt
      781 tgggtacaaa acaaaaatta gctttgcttc taactctcgt tacggaacct gatatattga
      841 ttttagatga accgactaat ggtttagata ttgaatcatc acaaatagtt ttagcggttc
      901 taaaaaattt agctttacat gaaaatgtgg gaattttaat atcgagtcat aaattagaag
      961 atattgaaga aatttgtgag agggttcttt tcttggagag cgggcttttg acatttcaaa
     1021 aagtaggaaa agatagtcat aatttcttgt ttgagatagc tttttcatca gctacagata
     1081 gagacatttt cattaccaaa caagaatttg gagatattgt tcaggaagag ggactgagaa
     1141 ttactatgtc tgggaatatt caaagtagtg agctttttaa attttttaac gaaaactcta
     1201 ttaaagtagt tgattttgaa actaaaaaag agacgcttaa agatatttac ctaaatcgtt
     1261 caaaataaag gaaggttata atcatgaaat taaataaatt gaattttctt aaggaaagta
     1321 taagaaattt atattcatca ggcgtaatat atctcagttt gcttatctcg tttataccgc
     1381 cgatattgct tacattcttt attctaaaga ctcaagggac atcgcttggt attaagcata
     1441 tttcaaactt ttatgccatg ctcggtatgt taatggctgt tatacatgct aatcgagtca
     1501 ttagtagaga tttttcccac aatacggtaa gtttgtttta taatcaacag aagaatcgga
     1561 tgatttacgt cttgtctaat tttctatatg ccatctcagt ttccattatt tatgcttgga
     1621 atggcattgt gttactagtc atagtaagta aattgggtat tccaggtgat ttaggattag
     1681 attttatagt agctattgta gtcaatacaa ttttgttagt cctgttttat tttctattat
     1741 cctacatttt ctatttatac aaattgaaaa gtggcttggt atttggtatt ttagtagctt
     1801 tactactctt tatccctaat atattaaata cgatgatgat gaatactagt aatgatttgt
     1861 ttatcaaagc aattgaactt cttccttttt attctttacc tgtatttgtg gcttcaaata
     1921 cgatgtctat tagtcagtat cttgtgctaa tcactacaat cattttattg tactttttca
     1981 ctctcaagaa aagcaagaag tattcatttt agtttcattt agtattattt tgcataccta
     2041 aaatacagta aaaaatcagt catcttggta tgctcctgct ttcactattc aacacgtttt
     2101 tgacttatac taggctcatt tccaa
//
LOCUS       DAVKQB010000109         2106 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKQB010000109 DAVKQB010000000
VERSION     DAVKQB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.109"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(233..544)
                     /locus_tag="VW528_002120"
     CDS             complement(233..544)
                     /locus_tag="VW528_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912867.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCSFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(559..1845)
                     /locus_tag="VW528_002121"
     CDS             complement(559..1845)
                     /locus_tag="VW528_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9912868.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFATKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDVY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
BASE COUNT          641 a          455 c          364 g          646 t
ORIGIN      
        1 agacgcaatt ctaatctttt agactttgca taataactgt taaacagttg aaaattaacc
       61 cagttttaac aagtagtcaa aataccagat aaaggaaaaa agtctttaaa atcttaaaaa
      121 cgcatagtat caggtattga acaatcttga tattatgcgt tttattgtgg gaagatttac
      181 ttcattttct cttggaattg agttttctcc tacttcaaaa ctagatgatt tgctatttaa
      241 aatagcgttc cttatgattt tggcaacctg gattaaagcg agaaaaacaa aaggagcaac
      301 ttaaactttc tttatattgc ttatatgtta gtagttttag acaaacacca cataaaatag
      361 gcttatcctg tatcaaagat aagggatacg gctcaaaagg gtgattttct aatgaatcat
      421 gacaccgata acaagcatag tactttttac aatcatagca ttgtaaagca ataatatcca
      481 acttgctatg gtaatgaatg catcttgttt gcttatccac taagaaaccc tgtgcctcta
      541 tcatttcctt ttcctttctt aagcacgaac tgtgacgctg gttccatctt ccttataaaa
      601 attgatgagt ccttctttta atgcacgaac catgtctcct gattgaacgg gttgtgggac
      661 tttaatagtc aatagttcca ttggatttgg agcgcggtcg attttattgc ctttggcatc
      721 atgcaaatct tcaatatagg tttcaaaatg acggaaacct ggaccataaa actcaacttg
      781 gtccccttcg ttaatgacgt ttcgttgacg aatggttgct gtttgtgccg catcatcata
      841 agaaaccact tcagcgacaa acttgtactc aggaatttta cggcgagcac caaacaactg
      901 ctcattttca gatggtatac cgtagtagaa acctgttgcc aattcacgtt gggcaacctt
      961 ccacatctcg tccaccaagt cttgtttgat agcttcaaac ttttcaggac tttcaagata
     1021 gacatccaca gccgccttgt agcagttggt tactgttgat acgtagtgaa tagacttcat
     1081 acgtccttcg atttttagac tgtccacacc attttcaatc atatctggaa tatggtcaat
     1141 catagacatg tcaacggctg acattgaaaa ttcttctgga atctcaccct gcaaactctt
     1201 acgttctttc ccaaatggca tatcgtaaag gtcgtatttc caacggcatg actgagaaca
     1261 tccaccacgg ttggcatcac gcatactcat gtggtttgaa agagtacaac gtccagagta
     1321 tgaaatacac atagctccat ggacaaaggc ttcaatttca acatctgtac gtttgcggat
     1381 ttcagctaat tcttccattg aaacctcacg cgctaaaacg acacgagtca agcctagctc
     1441 tttccagaac tcaagggttt catagttagt ggcactggct tgggtagaaa ggtggatttc
     1501 aaggcctggt gcttcagtcg ctgcaatcat aatcaaggct gggtcagata cgataactgc
     1561 tgcaatcccg atatcacgca gtttacggaa ccactcacca gcaccagctt catttccttc
     1621 gtgcataacc atattagccg ctacatagac cttggcacca tacttggtcg caaactgcac
     1681 gccttcttcc atctgttcga aagtaaagtt tcccgcacgg ctacgaagac cataggcctg
     1741 accaccgata aagacagcat ctgctccata ctgaacagct acctttagct tctctaaagt
     1801 ccctgcaggt gataaaacct caggacgttt taatgttttt gtcatgattt ctcctatttg
     1861 caatataaaa gtttgacatt tttccttctc attttatagt aaataaagga taaaatcaag
     1921 cctagacgga ttgttttgac aattctaatc ttttagactt tgcataataa ctattaaaca
     1981 gtcgaaaatt accccagttt taacaagtag tcaaaaagtc taaaaaagta aaaaccagta
     2041 tcccaagata ctgactcgta aaaatatata aattagtcct tagatgacta ctcccactct
     2101 gttgct
//
LOCUS       DAVKQB010000110         2031 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKQB010000110 DAVKQB010000000
VERSION     DAVKQB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.110"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1062..>2031
                     /locus_tag="VW528_002122"
     CDS             1062..>2031
                     /locus_tag="VW528_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEU9912869.1"
                     /translation="MDALKRTIANDIRPKQAQAEAEIAKQAEALSRTKNELAGASTLI
                     AQEAKRIGLDSVARLEAFKLQTTSAQTALSGDLDALKRTIANDIRPKQAQAEAEIAKQ
                     VEVLSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELSSKIAS
                     VQVGGRNYIRGTKRMMLARGLWASGTFRPSGAGTAKTIDVLDSPATGFDKAIRLTSSN
                     ARDQIGIAQDGFYISQGTYTMSCWVKGRRGQKVKLQTYWQVNDNSGISPIFTLKDENW
                     TKLSFTSARNRAGVASIGYVYLVNAEVGEYLDVLAPQLEDGSLATSS"
BASE COUNT          610 a          409 c          533 g          479 t
ORIGIN      
        1 ccacacttga acttatggac ggtatgttta agctcaatcg tgagtacgtg acggatttgc
       61 atttcccagc tgaagtacga gaggttattc aggaaatctg cctgaaaaca ggcattgagt
      121 tagcgaatga ctatttcgga atcagcgcga tgcgttatca tattgagcaa gttcctgagg
      181 gcaagaaact ttcctttagg gatatgctga gcgctatgac tcaggtgatt gggatgtctt
      241 gtttcttcaa cagagaaggc aagatggaaa tccgtgattt gactgagtcc aatatcacga
      301 tcaacgctga cagttatttc ttgcatggct tgaccaagag tgagattgag tatcagatag
      361 ctggtatcac ttgtaagacg gacaagaagt ctctgacggt cggtatgaag acaggccggt
      421 ctttggaact ggacaatgtc ttcatgaccc agagcgcttt aaatgacctg tattacaaac
      481 tgaaaaacct aacttactat ccgtataatc tcaactacca agggcattta ctgcttgagg
      541 tcgggcagtg ggtaaccatt cagaccaaca agaaagagac ttttaaagtt cctgtcttaa
      601 gtcagagctt tatctttaaa ggtggtctga gaggtcgtat cagtgcagat agtaaggctg
      661 gaaacgatac ccagtattct tacgagggta cgattaccaa gcagattaag caacaagatg
      721 gctttgaagc caaaatccaa gcgcagatag aagcagcaga cgcagccttt gacgctgaat
      781 ttgacaaacg tgagaaagct atcacggatg ccatcgaact tgccaaagcc agagctgaag
      841 aagtcaagag agaactgtct gacactatca atcagcgctt taatagcttt gacaacgggc
      901 cattgaaaga aactaagcgc aaggctgagg aagctttgcg aaatgctggc gcaagtaccc
      961 tgcttgcaca ggaagctaag cggattgggc tggattctgt cgctagactt gaagcgttta
     1021 agtcgcagac tacgagcgca caaacggctc tgtcgggtga cttggacgct ctgaaacgga
     1081 ctatcgcgaa tgatattcga ccgaagcaag cacaggctga agctgagatt gccaagcaag
     1141 ctgaagcact tagccggact aaaaatgaac tggctggcgc aagtacccta attgcacagg
     1201 aagctaagcg gattggactg gattctgttg ctagacttga agcgtttaag ttgcagacta
     1261 cgagcgctca gacggctttg tcaggtgact tggatgccct gaaacgaact atcgcaaacg
     1321 atattcgacc gaagcaagca caggctgaag ctgagattgc caagcaagtt gaagtactta
     1381 gtcggactaa gaatgaactg gctggcgtga agtcagcgca agcgacgtat gaggagacga
     1441 cgactcgtag actgtcagaa ctgaccaact tggccaatgg taaagccagc aagtcagaac
     1501 tcacgcagac agctgaggag ctctctagta agatagcgag tgtgcaggtc gggggtagga
     1561 attatatccg gggtacaaag cgcatgatgt tagccagagg attgtgggca tcaggtactt
     1621 ttagaccgtc aggcgctggg acggcaaaga cgattgatgt attagacagt ccagcaactg
     1681 gctttgataa agcgatacga ttgacctcaa gcaatgctag agaccaaata ggtattgctc
     1741 aagatggatt ttatatctcg caaggcacat acacgatgtc ttgttgggtc aaaggcagaa
     1801 gaggtcaaaa ggtcaagcta caaacttatt ggcaagtcaa tgataattcg ggtatttcgc
     1861 ccatctttac attaaaggat gaaaattgga caaagctatc gtttactagc gctagaaata
     1921 gggctggagt cgcatcaatt ggctatgtgt atctcgtaaa tgctgaagtc ggagaatatt
     1981 tagatgttct tgcgccccag ctggaagacg gaagtttggc aacaagctca a
//
LOCUS       DAVKQB010000111         1855 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKQB010000111 DAVKQB010000000
VERSION     DAVKQB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.111"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            16..88
                     /locus_tag="VW528_002123"
     tRNA            16..88
                     /locus_tag="VW528_002123"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49..51,aa:Asp,seq:gtc)
     gene            113..185
                     /locus_tag="VW528_002124"
     tRNA            113..185
                     /locus_tag="VW528_002124"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:146..148,aa:Lys,seq:ttt)
     gene            191..272
                     /locus_tag="VW528_002125"
     tRNA            191..272
                     /locus_tag="VW528_002125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:225..227,aa:Leu,seq:tag)
     gene            291..363
                     /locus_tag="VW528_002126"
     tRNA            291..363
                     /locus_tag="VW528_002126"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:324..326,aa:Thr,seq:tgt)
     gene            386..457
                     /locus_tag="VW528_002127"
     tRNA            386..457
                     /locus_tag="VW528_002127"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:418..420,aa:Gly,seq:gcc)
     gene            465..550
                     /locus_tag="VW528_002128"
     tRNA            465..550
                     /locus_tag="VW528_002128"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:499..501,aa:Leu,seq:taa)
     gene            560..633
                     /locus_tag="VW528_002129"
     tRNA            560..633
                     /locus_tag="VW528_002129"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:594..596,aa:Arg,seq:acg)
     gene            649..722
                     /locus_tag="VW528_002130"
     tRNA            649..722
                     /locus_tag="VW528_002130"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:683..685,aa:Pro,seq:tgg)
     gene            728..801
                     /locus_tag="VW528_002131"
     tRNA            728..801
                     /locus_tag="VW528_002131"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:762..764,aa:Met,seq:cat)
     gene            815..888
                     /locus_tag="VW528_002132"
     tRNA            815..888
                     /locus_tag="VW528_002132"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:849..851,aa:Ile,seq:cat)
     gene            899..988
                     /locus_tag="VW528_002133"
     tRNA            899..988
                     /locus_tag="VW528_002133"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:935..937,aa:Ser,seq:tga)
     gene            999..1072
                     /locus_tag="VW528_002134"
     tRNA            999..1072
                     /locus_tag="VW528_002134"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1033..1035,aa:Met,seq:cat)
     gene            1076..1148
                     /locus_tag="VW528_002135"
     tRNA            1076..1148
                     /locus_tag="VW528_002135"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1109..1111,aa:Phe,seq:gaa)
     gene            1168..1238
                     /locus_tag="VW528_002136"
     tRNA            1168..1238
                     /locus_tag="VW528_002136"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1200..1202,aa:Gly,seq:tcc)
     gene            1274..1347
                     /locus_tag="VW528_002137"
     tRNA            1274..1347
                     /locus_tag="VW528_002137"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1308..1310,aa:Ile,seq:gat)
     gene            1354..1441
                     /locus_tag="VW528_002138"
     tRNA            1354..1441
                     /locus_tag="VW528_002138"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1388..1390,aa:Ser,seq:gct)
     gene            complement(1574..>1855)
                     /locus_tag="VW528_002139"
     CDS             complement(1574..>1855)
                     /locus_tag="VW528_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9912870.1"
                     /translation="KEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYS
                     NAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT          437 a          386 c          490 g          542 t
ORIGIN      
        1 ccgttaactc ccaaaggtcc cgtagtgtag cggttatcac gtcgccctgt cacggcgaag
       61 atcgcgggtt cgattcccgt cgggaccgtt taaggtaacg caagttattt tagactcgtt
      121 agctcagttg gtagagcaat tgacttttaa tcaatgggtc actggttcga gcccagtacg
      181 ggtcatatat gcgggtttgg cggaattggc agacgcacca gatttaggat ctggcgctta
      241 acggcgtggg ggttcaagtc ccttaacccg cattaagata taacaaatga gccggcttag
      301 ctcagttggt agagcatctg atttgtaatc agagggtcgc gtgttcaagt catgtagccg
      361 gcatttttag atagaagaaa acagtgcgaa cgtagttcag tggtagaaca ccaccttgcc
      421 aaggtggggg tcgcgggttc gaatcccgtc gttcgcttag agaggccggg gtggcggaac
      481 tggcagacgc acaggactta aaatcctgcg attggtaacg atcgtaccgg ttcgattccg
      541 gtcctcggca taatatggag cacccttagc tcaactggat agagtacctg actacgaatc
      601 aggcggttag aggttcgact cctctagggt gcatttttct atttaactcg ggaagtagct
      661 cagcttggta gagtacttgg tttgggacca aggtgtcgca ggttcgaatc ctgtcttccc
      721 gattcatggc ggtgtagctc agctggctag agcgtccggt tcatacccgg gaggtcgggg
      781 gttcgatccc cttcgccgct ataatgatct tgtcggacct ttagctcagc tggttagagc
      841 tctcggctca taaccgagtg gtcgtaggtt caagtcctac aaggtccatt tgaatattgg
      901 aggattaccc aagtccggct gaagggaacg gtcttgaaaa ccgtcaggcg tgtaaaagcg
      961 tgcgtgggtt cgaatcccac atcctccttt tatattaacg cgggatggag cagctcggta
     1021 gctcgtcggg ctcataaccc gaaggtcgta ggttcaaatc ctgctcccgc aataaggctc
     1081 ggtagctcag ttggtagagc aatggattga agctccatgt gtcggcggtt cgattccgtc
     1141 tcgcgccatt ttattgatag tatttatgcg ggtgtagttt agtggtaaaa ctacagcctt
     1201 ccaagctgtt gtcgcgagtt cgattctcgt cacccgcttt gaactttgtt caaattacca
     1261 agttttttaa cttgggcgcg tagctcaggt ggttagagcg cacgcctgat aagcgtgagg
     1321 tcggtggttc gagtccactc gtgcccatta attggagaat tactcaagag gctgaagagg
     1381 acggtttgct aaatcgttag gtcgggtaac tggcgcaagg gttcgaatcc cttattctcc
     1441 gttttaatga gagtttgtaa ctctcttttt tatttttaga gacaataaag cttcagatcc
     1501 gtaaaactta attgaattac taatctgaag ttatttttag gctctttgtc aactgtagtg
     1561 ggttgaaaaa aagctaagct cgagaaagga caaattttgt cctttctttt ttgatgttca
     1621 aagcgataaa aatccttttt ttgaagtttt caaagttccg aaatccaaag gcgtttcgtt
     1681 tgataagttt gatgagatta tttgtcgctt ccagtttggc gttagaatag tgtagttgaa
     1741 gggcgttgac aatcttttct ttatctttga ggaaggtttt aaagacagtc tgaaaaatag
     1801 gatgaacctg cttcagattg tcctcaataa gcccgaaaaa tttctctggt tcctt
//
LOCUS       DAVKQB010000112         1794 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKQB010000112 DAVKQB010000000
VERSION     DAVKQB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.112"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            370..846
                     /locus_tag="VW528_002140"
     CDS             370..846
                     /locus_tag="VW528_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9912871.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            846..1607
                     /locus_tag="VW528_002141"
     CDS             846..1607
                     /locus_tag="VW528_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU9912872.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSSVYDSGENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT          661 a          240 c          348 g          545 t
ORIGIN      
        1 atatcagtaa aaacaacact ttattgaggt attggatacg ctttgctaat agcctaataa
       61 tcacatgtgg agtgttgcta caacgaaaaa ggtgataatc cttaatttca agctatttta
      121 taagcatttt gtctttgtag ataaaggcaa ttttgacaat aaaaatccta aaaggtgaat
      181 cgttatagat gtatttgtag atattgtttg cgcatcgaaa aaattaatac aagaataaat
      241 atttatagct ctttaggtga cttttataga agtaaagttt aggatagaaa aacaagaaat
      301 aacgcaccat ttttggtgcg ttatgctttt ttatgctata atggatttat aaaaataaag
      361 gagtttgcta tgattggaaa gaacataaaa tccttacgta aaacacatga cttaacacaa
      421 cccgaatttg cacgaattat aggaatttct cgaaatagtc tgagtcgtta tgaaaatgga
      481 acgagttcag tctctaccga attaatagac atcatttgtc agaagtttaa tgtatcttat
      541 gtcgatatcg taggagaaga taaaatgctc aatcctgttg aagattatga attgactttg
      601 aaaattgaaa ttgtgaaaga aagaggtgct aatctattat ctcgactcta tcgttatcaa
      661 gatagtcagg gaattagcat tgatgatgaa tctaatcctt ggattttaat gagtgatgat
      721 ctatctgact tgattcagac aaatatctat ttagtagaaa attttgatga aatagagaga
      781 tatagcggct atttggatgg aattgaacgt atgttagaga tatctgaaaa gcggatggta
      841 gcttaatgga aatccaagat tatactgata gtgaattcaa acatgcttta gcaaggaatc
      901 ttcgttcact gacaagagga aaaaagtcca gtaaacaacc tatagcgatt ttgcttggag
      961 gtcaaagtgg tgccggtaag actacaattc atcgtattaa acagaaagaa tttcaaggaa
     1021 atattgttat catagatggt gatagttttc gttctcagca tccacactat ttagaactgc
     1081 agcaagaata tggcaaagac agtgtagaat ataccaaaga ttttgcagga aaaatggtag
     1141 agtctttagt aacaaaattg agtagtttga gatacaatct tttgatcgag ggaactttac
     1201 gaacagttga tgttccaaag aaaacagcac aactcttgaa aaataaggga tatgaagtac
     1261 aattggcctt aattgcgaca aagcctgaat tgtcgtatct aagtactctt atccgttatg
     1321 aagaactgta cattatcaat ccaaatcaag cacgcgcaac tccaaaagaa catcatgatt
     1381 tcattgtaaa tcatctagtt gataatacac gacaattgga agaactggct atctttgaaa
     1441 gaattcaaat ttatcaacga gatagaagtt ctgtatatga ttcaggagaa aatacaactt
     1501 cagcagcaga tgttcttcaa gagttactgt ttggggagtg gagtcaggta gagaaggaga
     1561 tgttgcaggt gggggaaaag agacttaatg aattacttga aaaataaaca attgatattt
     1621 ttaggagagt agaaatgaga gggtttaata acaagataaa gtctgtttat caagaactaa
     1681 caaattccaa agagaaattc ggtagctttc acaagacttt aattcatttg catacacctg
     1741 tttcttatga ttacaagcta ttttctaatt ggactgcaac gaaatataga aaaa
//
LOCUS       DAVKQB010000113         1778 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKQB010000113 DAVKQB010000000
VERSION     DAVKQB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.113"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(615..1595)
                     /locus_tag="VW528_002142"
     CDS             complement(615..1595)
                     /locus_tag="VW528_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9912873.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDDFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKKGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT          556 a          371 c          332 g          519 t
ORIGIN      
        1 tttcaaaaaa ttcttttata taataatgtc cttgaaagta gtttcagctg aacatttata
       61 gtccataaaa agaaaacaga aactcatctg tttttatact caatgaaaat caaagagcaa
      121 actaagaagc tagccgcatg atgctcaaaa cactgttttg aggttgtaga taagactgac
      181 aaagtcagct caaaacaccg ttttgaggtg gcagatagaa ctgacgaagt cagtaacata
      241 tatacggcaa ggcgaagctg acgtggtttg aagagatttt tgaaaagtat taacacctac
      301 agacaagcct aacgatagca agaactaccc tactcgatag gtatcggctt ttgctttctg
      361 aaaaaaatta ttttaagcaa tttgacaaat ctagcaacaa aaaattctat aaatataata
      421 gattgaaact agaatagtac acatctactt ctaaaacatt gttagaaatc gatttgactc
      481 tcctgatcga tttgtcctgt tcttgtttca ttttactata tttccatgat aaaacgcata
      541 gtatcaagtt ttcttaatcc cctgatacta tgcgtgtttg taatttttaa gattttgtgc
      601 ttagagtcga ctccttattt tagatattta aaaggaatct cacttccaca gagccagttg
      661 tagacttggt cattgacaaa tacattcatg gcttcgtgag catactcagg catgatgcga
      721 taggttttat cgcaggtcag acgattataa atcgcaaact gggtaatggg atagcaaaca
      781 tcgtcgtcca agcccgtaat catcttaacc tcaccttgga tacgatgggc aagatttttg
      841 acatcgatat aggcaagggt cgccatgatt tcctcctctg tttcatggaa ggggtcgtga
      901 aacttgaaat aacggaaaag ttcgtcgtaa gcctcgctag tattaccaat ctcaatcacc
      961 cgtctgaagt ctgacaagaa gggataaatg gcaactgttt tctgaattcg aggattgagc
     1021 gctgctgcaa ctagagctag agcccctcct tgtgaggcac catagctaga aagacgcttc
     1081 tcatcaacct gagacagact agcaacaatt tcgaccaact ggtaaatatc cagataaaca
     1141 tccttataaa agaggtggtc ccgaccttcc acagcaccac ggataatatg ccccttcacg
     1201 gtatttccta aaggagaacg caagccgtct tgtgagtaac ctgactggcc ccgcacatcc
     1261 atggaaacaa caccgtaacc agctacggtg aagcccagca tgtcggccca gtcccagcca
     1321 cgtcccatat aaccatgaaa atggaagatt agtggaacct tctcctcact ctttggaaga
     1381 acaatgcgtg catagacctt tcctttcttg cttccttcaa atgttaactc atagcacttg
     1441 acttgaggaa tgtggaaatc tctttcctcc aagtggtagg atggaagcgt ggaaacattt
     1501 ttcacttccc catcccagaa atcatcaaag tcttccggaa cctcatccct tcctctatag
     1561 gtcttaattt cttctagcaa agctggattt ttcatagcat gctcctttta ttttgtaatc
     1621 gttaccacaa ctgtagcata tctatgtaat caatgcaaga aagaagggta aatagaaagt
     1681 gaatttagat tctttacttt taggatgatg ggctgaaagt agttttagaa acgaaaaaag
     1741 agcatttcgc tctctcttct gtttatacta gttttttg
//
LOCUS       DAVKQB010000114         1730 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKQB010000114 DAVKQB010000000
VERSION     DAVKQB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.114"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>1730
                     /locus_tag="VW528_002143"
     CDS             <1..>1730
                     /locus_tag="VW528_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEU9912874.1"
                     /translation="ELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFG
                     ISAMRYHIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADS
                     YFLHGLTKSEIEYQIAGITCKTDKKSLTVGMTTGRSLELDNVFITQSALNDLYYKLKN
                     LTYYPYNLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFIFKGGLRGRISADSKAG
                     NDTQYSYEGTITKQIKQQDGFEAKIQAQIEAADKDFDQKVDKIKKDFNDQVELAKARA
                     EEVKRELSDTINQRFNSFDNGPLKEAKRKAEEALRNAGASSSLAQESKRIGLDSVARL
                     EAFKSQTTSAQTALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEALSRTKNELDGAS
                     TLLAQEAKRIELDSVARLEAFKSQTTSAQTALSGDLDVLKRTIANDIRPKQAQAEAEI
                     AKQVEALSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELASR
                     IASVQASGRNLFLNSLFKQDISKTGIWTTSTYTATIDSESKYLGYNALKIIGLNPSGR
                     DGGNPKVTYP"
BASE COUNT          542 a          345 c          431 g          412 t
ORIGIN      
        1 tgaacttatg gacggtatgt ttaagctcaa tcgtgagtac gtgacggatt tgcatttccc
       61 agctgaagta cgagaggtta ttcaggaaat ctgcctgaaa acaggcattg agttagcgaa
      121 tgactatttc ggaatcagcg ctatgcgtta ccatatcgag caagttcctg aaggtaagaa
      181 actttcgttc agggatatgc tgagcgctat gactcagatg attgggatgt cttgcttctt
      241 caaccgagaa ggaaagatgg aaatccgtga tttgaccgag tccaacatca cgattaacgc
      301 tgacagttac ttcttgcatg gattgaccaa gagtgaaatc gagtatcaga tagctggtat
      361 cacttgtaag acggataaga agtctctgac ggtcggtatg acaacaggtc ggtctttgga
      421 acttgataat gtcttcataa cccagagcgc tttaaatgac ttgtattaca aactgaaaaa
      481 cctaacttac tatccgtata atctcaacta ccaaggacat ttgttacttg aggttgggca
      541 gtgggtaacc attcagacca acaagaaaga gacttttaaa gttcctgtct taagtcagag
      601 ctttatcttt aaaggtggtc tgagaggtcg tatcagtgca gatagtaagg ctggaaacga
      661 tacccagtat tcttacgagg gtacgattac caagcagatt aagcaacaag atggctttga
      721 agcgaaaatc caagcgcaga ttgaagcagc agataaagat tttgaccaaa aggtcgacaa
      781 aatcaaaaaa gactttaacg atcaagtaga actggccaaa gccagagctg aagaagtcaa
      841 gagagaactg tctgacacta tcaatcagcg ctttaatagc tttgacaacg ggccattgaa
      901 agaagctaag cgcaaggctg aggaagcttt gcgaaatgct ggcgcaagta gttctcttgc
      961 tcaggaatcc aagcggattg ggctggattc tgttgctaga cttgaagcgt ttaagtcgca
     1021 gactacgagc gcacaaacgg ctctgtcggg tgacttggat gttctaaaac gaactatcgc
     1081 aaacgatatt cgaccgaagc aagcacaggc tgaagctgag attgccaagc aagttgaagc
     1141 acttagccgg actaaaaatg aactggatgg cgcaagtacc ctgcttgcac aggaagctaa
     1201 gcggattgag ctggattctg ttgctagact tgaagcgttt aagtcgcaga ctacgagcgc
     1261 acaaacggct ctgtcaggtg acttggatgt tctaaaacga actatcgcaa acgatattcg
     1321 accgaagcaa gcacaggctg aagctgagat tgccaagcaa gttgaagcac ttagccggac
     1381 taaaaatgaa ctggctggcg tgaagtcagc gcaagcgacg tatgaggaga cgacgactcg
     1441 tagactgtca gaactgacca acttggccaa tggtaaagcc agcaagtcag aactcacgca
     1501 gacagctgag gagctagcta gtcggattgc gagtgtgcag gcatccggtc gaaatctatt
     1561 cttgaactca ctattcaagc aggatatttc aaaaacagga atttggacaa cgagtacata
     1621 tacggctact atcgatagcg aaagtaagta tcttggatat aacgctctta aaattatagg
     1681 tctgaatcca tctggccgtg atggagggaa tcccaaggtt acttatccag
//
LOCUS       DAVKQB010000115         1726 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKQB010000115 DAVKQB010000000
VERSION     DAVKQB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.115"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..213)
                     /locus_tag="VW528_002144"
     CDS             complement(<1..213)
                     /locus_tag="VW528_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9912875.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     GKEAFRHGHGNRNRKPKHAIPDEIKER"
     gene            377..529
                     /gene="rpmG"
                     /locus_tag="VW528_002145"
     CDS             377..529
                     /gene="rpmG"
                     /locus_tag="VW528_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9912876.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            539..715
                     /gene="secE"
                     /locus_tag="VW528_002146"
     CDS             539..715
                     /gene="secE"
                     /locus_tag="VW528_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9912877.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            770..1306
                     /gene="nusG"
                     /locus_tag="VW528_002147"
     CDS             770..1306
                     /gene="nusG"
                     /locus_tag="VW528_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9912878.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
     gene            1428..>1726
                     /locus_tag="VW528_002148"
     CDS             1428..>1726
                     /locus_tag="VW528_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9912879.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGELSHRISEGG"
BASE COUNT          558 a          258 c          367 g          543 t
ORIGIN      
        1 acgttctttg atttcatcag ggattgcatg ttttggtttt cgatttctgt ttccgtgtcc
       61 gtgtctgaag gcttcttttc ctttctgttg ataggctagt aacagacgat tgatttgtct
      121 ttcagaaaga ttgagttcga cacaggcccg tttctttgtt ttctttcctt gggctatagc
      181 ttttatcaca agatattttt tcgtttcatt catattcagt tggatccttt tcatatgact
      241 attctaccaa atgggacatt ttcacgttcg atttactaaa gacattatca cattcgaatc
      301 acaatagtca atctatttcg ctaagatgct tgtcaaagcc tagctttctt gttataatgg
      361 ataagatgga ggcgttatgg cactaaaaaa agcaagccta gcttgtgcgg tttgtggttc
      421 gagaaactat tcaatcaaga tcagcggaaa ccccaagcct acacgactag aagtaaataa
      481 attttgtaag cattgtggca agtacactac acacagagaa acgagatagg agagagcgat
      541 gcgttttatt ggagatattt ttagacttct taaagacaca acgtggccaa ctcgcaagga
      601 aagctggaga gattttcgtt ctatcatgga atacacagct ttctttgtag taattattta
      661 catttttgac cagttgattg tttcaggttt gattcgattt attaacattt tttagaagat
      721 tagtggagtt aattacacta gaaatcttct atttatgaaa ggaaatatca tggatagttt
      781 tgataaaggg tggtttgttt tacaaactta ttctggttat gaaaataagg tgaaagaaaa
      841 tctattacaa cgtgcacaaa cctacaatat gttggataat attctacgcg ttgaaattcc
      901 aacacaaaca gtgcaagttg aaaaaaatgg aaagagaaaa gaagtagaag aaaatcgctt
      961 tccaggttat gttcttgtag aaatggtcat gacagatgaa gcttggtttg ttgttcgaaa
     1021 cacaccaaat gttacaggat ttgtcggatc acacgggaac agatcaaaac caactccatt
     1081 attggaacaa gaaattcgtg acattttggt atctatggga caaactgttc aagaatttga
     1141 tttcgatgtt gagattggtc aaactgtacg tattattgat ggtgcttttg cagactacac
     1201 tggtaagatt acagaaattg ataataacaa agtgaaaatg attatctcta tgtttggtaa
     1261 tgacacagtt gcagaagtaa acctaaacca aatcgcagaa ttataaccct gagagaggct
     1321 ggagcctctc ttttttgtgc agtttaggag ctaaagggaa cagaatggag aaaatggaac
     1381 aaatgtgttt tctaatctgt tagactgtat ctagaaaggg gaaaattatg attaaagaat
     1441 tgtatgaaga agtccaaggg actgtgtata agtgtagaaa tgaatattac cttcatttat
     1501 gggaattatc ggattgggac caagaaggca tgctctgctt acatgaattg attagtagag
     1561 aagaaggact ggtagacgat attccacgtt taaggaaata tttcaaaacc aagtttcgaa
     1621 atcgaatttt agactatatc cgtaagcagg aaagtcagaa gcgtagatac gataaagaac
     1681 cctatgaaga agtgggtgag ctcagtcatc gtataagtga gggggg
//
LOCUS       DAVKQB010000116         1564 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKQB010000116 DAVKQB010000000
VERSION     DAVKQB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.116"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>1564
                     /locus_tag="VW528_002149"
     rRNA            <1..>1564
                     /locus_tag="VW528_002149"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          471 a          301 c          458 g          334 t
ORIGIN      
        1 aaggatgtga ggaggaagac gcagtgaact gaaacatcta agtagctgca ggaagagaaa
       61 gcaaaagcga ttgccttagt agcggcgagc gaaacggcag aagggcaaac cgaagagttt
      121 actcttcggg gttgtaggac tgcaatgtgg actcaaagat tatagaagaa tgatttggga
      181 agatcagcca aagagagtaa tagcctcgta tttaaaatag tctttgtacc tagcagtatc
      241 ctgagtacgg cgggacacgt gaaatcccgt cggaatctgg gaggaccatc tcccaaccct
      301 aaatactccc tagtgaccga tagtgaacca gtaccgtgag ggaaaggtga aaagcacccc
      361 gggaggggag tgaaatagaa cctgaaaccg tgtgcctaca acaagttcga gcccgttaat
      421 gggtgagagc gtgccttttg tagaatgaac cggcgagtta cgttatgatg cgaggttaag
      481 ttgaagagac ggagccgtag ggaaaccgag tctgaatagg gcgccttagt atcatgacgt
      541 agacccgaaa ccatgtgacc tacccatgag caggttgaag gtgcggtaag acgcactgga
      601 ggaccgaacc agggcacgtt gaaaagtgct tggatgactt gtgggtagcg gagaaattcc
      661 aaacgaactt ggagatagct ggttctctcc gaaatagctt tagggctagc gtcgacatta
      721 gagattcttg gaggtagagc actgtttggg tgaggggtcc atcccggatt accaatctca
      781 gataaactcc gaatgccaat gaattatggt cggcagtcag actgcgagtg ctaagatccg
      841 tagtcgaaag ggaaacagcc cagaccacca gctaaggtcc caaaataatt gttaagtgga
      901 aaaggatgtg gggttgcaca gacaactagg atgttagctt agaagcagct attcattcaa
      961 agagtgcgta atagctcact agtcgagtga ccctgcgccg aaaatgtacc ggggctaaaa
     1021 caatttaccg aagctgtgga tacctttata ggtatggtag gagagcgttc tatgtgtgat
     1081 gaaggtatac cgtgaggagt gctggaacgc atagaagtga gaatgccggt atgagtagcg
     1141 aaagacaggt gagaatcctg tccaccgtaa gactaaggtt tccaggggaa ggctcgtccg
     1201 ccctgggtta gtcgggacct aaggagagac cgaaaggtgt atccgatgga caacaggttg
     1261 atattcctgt actagagtat gtagtgatgg agggacgcag taggctaact aaagcagacg
     1321 attggaagag tctgtctaag cagtgaggtg tgaattgagt caaatgctta attctataac
     1381 attgagctgt gatggggagc gaagtttagt agcgaagtta gtgacgtcac actgccaaga
     1441 aaagcttcta gcgtttaaac atactctacc cgtaccgcaa accgacacag gtagtcgagg
     1501 cgagtagcct caggtgagcg agagaactct cgttaaggaa ctcggcaaaa tgaccccgta
     1561 actt
//
LOCUS       DAVKQB010000117         1531 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKQB010000117 DAVKQB010000000
VERSION     DAVKQB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.117"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            29..1375
                     /gene="gdhA"
                     /locus_tag="VW528_002150"
     CDS             29..1375
                     /gene="gdhA"
                     /locus_tag="VW528_002150"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9912880.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV"
BASE COUNT          463 a          305 c          322 g          441 t
ORIGIN      
        1 taaaatatga caataaaagg agaacgatat gacatctgct aaagaatata tccaaagcgt
       61 gtttgaaact gtaaaagctc gtaacgggca cgaggctgaa ttcctccaag ctgttgaaga
      121 atttttcaac actttggaac ctgtatttga aaaacaccct gagtatatcg aagaaaatat
      181 cttggcacgt attactgagc ctgagcgcgt gatttctttc cgtgttcctt gggttgaccg
      241 tgatggaaaa gttcaagtaa accgcggcta ccgtgttcaa ttcaactcag ctgttggacc
      301 atacaaaggt ggacttcgtt tccacccaac tgtaaaccaa gggatcttga aattcctcgg
      361 atttgaacaa atctttaaaa atgtcttgac tggacttcct atcggtggag gtaaaggtgg
      421 atcagacttc gatcctaaag gtaaaacaga tgctgaagtg atgcgcttct gccaaagctt
      481 catgactgaa ttgcaaaaac acatcggacc atcacttgac gtacctgctg gtgatatcgg
      541 tgttggtgga cgtgaaattg gttaccttta cggtcaatac aaacgtctta accaatttga
      601 tgctggtgtc ttgactggta aacctcttgg atttggtggt agcttgattc gtccagaagc
      661 aactggttac ggtttggttt actatactga agaaatgctc aaagctaacg gtaacagctt
      721 tgctggtaag aaagtcgtta tttcaggttc tggtaacgtt gctcaatacg ctcttcaaaa
      781 agcaaccgaa cttggtgcaa cagttatctc tgtttctgac tcaaacggct atgttatcga
      841 tgaaaacggt atcgacttcg atcttttggt tgatgttaaa gaaaaacgtc gcgctcgttt
      901 gactgagtat gcagctgaga aagcaactgc tacttatcat gaaggttctg tatggactta
      961 cgctggaaac tatgacattg cgcttccatg tgctactcaa aacgaaatca acggtgaagc
     1021 agctaaacgt ttggttgctc aaggcgttat ctgtgtatct gaaggtgcca acatgccaag
     1081 caaccttgat gccatcaaag tttacaaaga aaatggtatc ttctacggac ctgccaaagc
     1141 tgccaacgct ggtggtgtag ccgtttcagc ccttgaaatg agccaaaaca gccttcgcct
     1201 ctcatggact cgtgaagaag ttgatggacg tctcaaagac atcatgacca acatctttaa
     1261 cacagctaaa acaacttcag aaacatatgg tcttgataaa gactaccttg caggagctaa
     1321 cattgctgcc tttgaaaatg tagcaaacgc tatgattgca caaggcgtgg tataaaaaaa
     1381 aattacggaa actgactacc cagacgggta gccagttttt tcaattccta aaatttaaga
     1441 ttctaatacc ctttatgcaa aaatatagaa ttctaccttt ttctttatat aggctaatgt
     1501 ttttaatata tttattgtct atttttgaaa a
//
LOCUS       DAVKQB010000118         1495 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKQB010000118 DAVKQB010000000
VERSION     DAVKQB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.118"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..91
                     /locus_tag="VW528_002151"
     CDS             <1..91
                     /locus_tag="VW528_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nickase"
                     /protein_id="HEU9912881.1"
                     /translation="VVSGKLKKLLSDKEKLSPKKWNEEKILLM"
     gene            417..590
                     /locus_tag="VW528_002152"
     CDS             417..590
                     /locus_tag="VW528_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912882.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
     gene            776..1303
                     /locus_tag="VW528_002153"
     CDS             776..1303
                     /locus_tag="VW528_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEU9912883.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            1430..>1495
                     /locus_tag="VW528_002154"
     CDS             1430..>1495
                     /locus_tag="VW528_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEU9912884.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRI"
BASE COUNT          789 a          191 c          232 g          283 t
ORIGIN      
        1 agtagtaagt gggaaattaa aaaaactcct atcagataag gaaaaactaa gtcctaaaaa
       61 gtggaatgaa gaaaaaattc ttttaatgta aaatcttgaa gaaataaata aagaaaaaga
      121 caagataaaa gatgaatacc aggaaattaa tcatataaaa tattcagtag attttgtaaa
      181 caaagaactt ggtatagatt tatccataga aatagataag ctaataaaac agggagaaaa
      241 accaagtgta atagcacaga ttaaaaagtt ccaagaccaa gtcaataaag ataatgaata
      301 cagagaaatg atgaaaaaca agaaaatgga tcaagaaaga taagacctgg taaaaatatc
      361 ttatccaagc tataatatga ccaaacaact aaagtcagat ctaggaggta atagagatga
      421 ataaaaggca agagctaata gatgaactca taaaagcaaa tgaagatgga acatacaaaa
      481 cctataaaag cacagaagaa ataaaagcaa tgaacaatga agaaatacag attatatatt
      541 ccaacatgaa aaactatcta tcagacaaaa gaacacacat aaactattaa aggtggaaag
      601 gtagtgtaag ctatttttaa aagtataaaa ggtacaaaga cctaagaaat atataaaaac
      661 atcaaaataa agtattccac caccaagaac aataaaataa aaacaagcaa agggaagtat
      721 agaaaaatta aaacctatac ttcccttttt taattcaaac aaaggagaga aaagcatgat
      781 aaacgaagaa ataagcaagg aagcaggtca agcagcacaa accataatat catacacaat
      841 aaaggcaaca aaagaatcaa tcaatttaga aaaagaaata agaaaaaaga tgaatgaaac
      901 tttagaaaaa gcaaatggaa acttaaaaag tcttatgggc gatgaaatga aaataaaaga
      961 tctctacaag aaaggacaac tagaaaatat aagcatagat caaatcgacc tcaaagactt
     1021 aaaaaaagaa ctaaacaaac ttggagtaag tttctcagta atgaaaaaca aagaaagcaa
     1081 aaactatgaa atattcttcc aagccaaaga cataaaagta atggaatatg cctttaagca
     1141 agtcatagcc aaggaaaata aaaaagaaaa agaaagtatc ctaaaacaaa taaagaaata
     1201 caaagaccta tccaaaaaca aagataagac aaaagaaaaa ggaaaaagga aagtaaaaga
     1261 aaaagtaaaa ccaaacaaaa aagatatgac cagagaaatc taaagggagt aacgaaaagt
     1321 tacaccctta aataaccata ataacaagac ttccgaaaaa cagaagtcca gaattccgaa
     1381 aatcggaaat caagaatttc gattatcgaa aatcaagaaa ggagcagata tggcaacaaa
     1441 caaaagatac tattggataa aattaaaaga agaatttttc acagacaaaa ggata
//
LOCUS       DAVKQB010000119         1442 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKQB010000119 DAVKQB010000000
VERSION     DAVKQB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.119"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..808
                     /locus_tag="VW528_002155"
     CDS             <1..808
                     /locus_tag="VW528_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9912885.1"
                     /translation="HQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERKRDGNSKTI
                     YKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQIIKIATDK
                     NDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSSNLLNKGIE
                     AYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNECLNESISLL
                     QNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS"
     gene            977..1423
                     /locus_tag="VW528_002156"
                     /pseudo
     CDS             977..1423
                     /locus_tag="VW528_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          528 a          226 c          265 g          423 t
ORIGIN      
        1 tcatcaaaac atttactttt attcaaagtc aaaagatttt aaatttaata caatttttac
       61 agagtattct tctactacaa atatcgacca aatactagtg gaacgaaaac gagatggaaa
      121 ctctaaaact atatataaag ttgataataa tggtaactat attctagcaa aagagaaaaa
      181 tggagttccc ctttcagatg tttggaatat accatttctt aatccaaaag ctaaagaaag
      241 agtaggttat cctacacaaa aacctattct gttattagaa caaattataa agattgctac
      301 tgataaaaat gatatagttt tagacccgtt ctgtggaagc ggaactactt tagtagcctc
      361 caagattttg aatagaaatt atatggggat tgatttatct gaggaagcta tcaatataac
      421 tcagcaacgt ctggaaaatg ttataaaaac aagttcaaat ttattgaata aaggaatcga
      481 agcatataga accaaaactg aggaagagga aaacattctt aaattattac aggcaaaaat
      541 tgttcaaaga aataaaggaa ttgatggttt tttacctaaa cattttcaaa aaaaaccgat
      601 acctataaaa attcaaaaaa ataatgaatg tctgaatgag agtatctctt tattacagaa
      661 tgctataaac tccaaaaaac ttgattttgg agtagttata aaaactcatt cagataattt
      721 attatttgat tttgatacga ttcctgaaaa tataatagta gttgaccatt ttgaacttac
      781 aatcgaaaaa tggttaagta aaagttaaca gttactatag tttgcaaatt atttacagaa
      841 agaacaagaa ttcctaggag attattctgg ctatgttcat tgtgacatgt tgcggcagta
      901 acttaggact ttagtcctct agttctgcct atgcgatagc agtccaaagt ttaggagtaa
      961 ggcgacgcta agcttggtaa actgcgaaca gctagaagct tatcgtcaac tggaagaagc
     1021 tgcacttgtt ggatgttggg cgcatgtgag aaggaagttt tttgaagcga cccccaagca
     1081 agcagataaa tcatccttag gagctaaagg tttagcttat tgtgatcagt tattttcctt
     1141 ggaaagagac tgggaggctt tgccagctga tgaacgacta cagaaacgtc aagaacatct
     1201 ccaaccccta atggaagact tctttgcttg gtgccgccgt cagtcagttt tagcaggttc
     1261 aaaactagga agggcaattg aatacagcct caagtataaa gaaaccttta agaccatttt
     1321 aaaagacgga catctggtcc tttccaataa tctagctgaa cgcgccatta aatcattggt
     1381 tatgggacgg agtaaaagag tccagtggac tcttttagcc tgagctcagt ttaaaaaagc
     1441 ga
//
LOCUS       DAVKQB010000120         1346 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKQB010000120 DAVKQB010000000
VERSION     DAVKQB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.120"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(100..816)
                     /locus_tag="VW528_002157"
     CDS             complement(100..816)
                     /locus_tag="VW528_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9912886.1"
                     /translation="MKKYFISGLGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFNTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(820..1131)
                     /locus_tag="VW528_002158"
     CDS             complement(820..1131)
                     /locus_tag="VW528_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912887.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
BASE COUNT          410 a          262 c          199 g          475 t
ORIGIN      
        1 aaaaataggc tccataatat ctatagtgga tttacccact acaaatatta tagagcctgt
       61 agattttcat cttataccat ttccagcaat acaagtagct catagaaaat aagcgagcca
      121 ctcattcatt agactagcga tttctttagg tgcttgagta taaagctcat ggccaaagtt
      181 ttctaaaaaa atagtattaa aatagtctgg caattctttt agggcttcct ctctccatgt
      241 agctccatta ggatagcgag gactaataaa caaggtatct cccacttctc tcttaaaagc
      301 ttgtattttt ctccgtaggc ggagtatcgc ttctatattt tcataattta tagccaactc
      361 atatctatta tactcaacat tccagtgata ggactgtctt acagctttct ccatattttc
      421 tgaccaatgc tttgcttcgg acttctcttt agaaataaga agagctaagt ccgaaacaat
      481 ttgagatttg atataatttt tagtttcctc taactctgta tccaaaggta aaatcttatc
      541 taaatctaga tagccaccat ccaaaagaat cagtttcttt acttcttcaa attccgatgc
      601 gaaataacga gctaaatctc ctccaagaga atggcctatc agacagatag attcttcctc
      661 tacaatctca tttttaaacc atgatttcaa ttctgtttca tctcgaagat gcttttcata
      721 tggatttaga aaatagacct gcgaatctag ttcttgaaaa aaatccttgc tatgataggc
      781 attgcttccc aaaccgctaa taaaatattt tttcattctc tacttaatac tatgcttatt
      841 catcttttgt tcaaagatag ttgtgataat ctgacgcaat tcttcgcgtt ctttttctgg
      901 aatctcacca cttgtttgag ctacagcgta gagttcaggg tattcaattg aaatgcgttt
      961 aatcgtacgt gttgtagcat gttttctgac aaaaaacggg attcgcttaa tcaagtcttg
     1021 tggtactagc gccagaatct tctcagtagt ttctttgtca ctaatattag acattgtaag
     1081 ccttttctta atcatttcct gttctttttc tgtaaaatct tttaattcca ttcgattagt
     1141 cctcctattt tctctaagtt aaattatgta ctaatacaga tgaaactaca aagaataaac
     1201 tttaagaaat cttctcactg ataagatttt agcattagac ttcctgcgaa acaaaatatg
     1261 gtatagtagt tctatgaatg atgaagcaag taaacaacta actgatgcac gatttaagcg
     1321 tcttgttggt gttcagcgta ccactt
//
LOCUS       DAVKQB010000121         1308 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKQB010000121 DAVKQB010000000
VERSION     DAVKQB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.121"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..1136
                     /locus_tag="VW528_002159"
     rRNA            <1..1136
                     /locus_tag="VW528_002159"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1213..>1308
                     /gene="rrf"
                     /locus_tag="VW528_002160"
     rRNA            1213..>1308
                     /gene="rrf"
                     /locus_tag="VW528_002160"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          350 a          277 c          382 g          299 t
ORIGIN      
        1 cccaagcaac tgtttatcaa aaacacagct ctctgctaaa tcgtaagatg atgtataggg
       61 ggtgacgcct gcccggtgct ggaaggttaa gaggagtgct tagcgtaagc gaaggtatga
      121 attgaagccc cagtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt
      181 gtcgggtaag ttccgacccg cacgaaaggc gtaatgattt gggcactgtc tcaacgagag
      241 actcggtgaa attttagtac ctgtgaagat gcaggttacc cgcgacagga cggaaagacc
      301 ccatggagct ttactgcagt ttgatattga gtgtctgtac cacatgtaca ggataggtag
      361 gagtctaaga gatcgggacg ccagtttcga aggagacgct gttgggatac tacccttgtg
      421 ttatggccac tctaacccag ataggtgatc cctatcggag acagtgtctg acgggcagtt
      481 tgactggggc ggtcgcctcc taaaaggtaa cggaggcgcc caaaggttcc ctcagaatgg
      541 ttggaaatca ttcgcagagt gtaaaggtat aagggagctt gactgcgaga gctacaactc
      601 gagcagggac gaaagtcggg cttagtgatc cggtggttcc gtatggaagg gccatcgctc
      661 aacggataaa agctaccctg gggataacag gcttatctcc cccaagagtt cacatcgacg
      721 gggaggtttg gcacctcgat gtcggctcgt cgcatcctgg ggctgtagtc ggtcccaagg
      781 gttgggctgt tcgcccatta aagcggcacg cgagctgggt tcagaacgtc gtgagacagt
      841 tcggtcccta tccgtcgcgg gcgtaggaaa tttgagagga tctgctccta gtacgagagg
      901 accagagtgg acttaccgct ggtgtaccag ttgtcttgcc aaaggcatcg ctgggtagct
      961 atgtagggaa gggataaacg ctgaaagcat ctaagtgtga aacccacctc aagatgagat
     1021 ttcccatgat tatatatcag taagagccct gagagatgat caggtagata ggttagaagt
     1081 ggaagtgtgg cgacacatgt agcggactaa tactaatagc tcgaggactt atccaaagta
     1141 actgagaata tgaaagcgaa cggttttctt gaattgaata gatattcaat tttgagtagg
     1201 tattactcag agttaagtga cgatagccta ggagatacac ctgtacccat gccgaacaca
     1261 gaagttaagc cctagaacgc cggaagtagt tgggggttgc cccctgtg
//
LOCUS       DAVKQB010000122         1299 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKQB010000122 DAVKQB010000000
VERSION     DAVKQB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.122"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            199..539
                     /gene="tnpB"
                     /locus_tag="VW528_002161"
                     /pseudo
     CDS             199..539
                     /gene="tnpB"
                     /locus_tag="VW528_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(572..1299)
                     /locus_tag="VW528_002162"
                     /pseudo
     CDS             complement(572..1299)
                     /locus_tag="VW528_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          395 a          264 c          237 g          403 t
ORIGIN      
        1 ggaaggcagt tcattctaca cccactatta tgcgttttta tcattttgaa atctactgct
       61 aatcaatgaa gaagcaaaga tcaaactagg cagctagtta caagttacgt aagcgcgtca
      121 taacaaggta tctatcctaa ttccccaact tccagtatac tagataaaaa ataattatcg
      181 gaggaaaggt attgtactat gataattcaa ctaagtgatt taggtcaagt tcaccttgtt
      241 tgtagcaaga tagatatgag gcagggaata gactcattag cctatgtagt taaaacccac
      301 tttgaattgg atcctttctc cggtcaagtc tttctctttt gtggtggacg taaagaccgc
      361 tttaaagccc tttactggga tggtcaagga ttttggctac tatataaacg ctttgagaac
      421 ggcagactga cttggcccaa tacagaaaag gatgtcaaag ctctcacacc tgaacaagta
      481 gactggctta tgacgggctt ttctatcact ccaaaaataa atccatcaga aagtcgtgat
      541 ttctattgaa atgaggactt tctttttata ctcatctgct ttcaaaaagc attctattcc
      601 atctccgatt aacgatggac tttatcacct ccttctccag tccttgtata acatcttgga
      661 gttgattcat gacatcttcc aaagttcgaa aggctttatt cttaaatcca cgtttacgaa
      721 tctctttcaa caattgttca atggggttca tctctggtgt gtatggagga ataaatgcaa
      781 agccaatatt agtcggaatc tttaaggtac ttgatttatg ccatacagca ttgtccataa
      841 cgagtaaaag ataatcatct ggataagctt gtgaaagctc ttctaaaaag gcgttcatcc
      901 actcagtatt acatccacca gctattaaga aaaatgattc gcctgtatag gcatcaacag
      961 ctccataaca atagcgaaat tctcgtatat agtgactatg gacatgtaga cctactccta
     1021 ttggagacca acaagatccc agtttactga ttctaccgaa accagcctca tcttggtaca
     1081 tcaagcaaac cttatgaaaa cgtctactgg ttttaaagcg ctttcttttc ttcttgaatt
     1141 gagattttat ttttagacgc gacaatggtt tgagcgtctg ctttcttagg atgttctgga
     1201 cgtggcataa tatttcgcca accatggcgc ttcaacagtt gatagaaggc atcacgtgtg
     1261 taggaacgac ctaactcctt tttataagcc tgaaataag
//
LOCUS       DAVKQB010000123         1282 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKQB010000123 DAVKQB010000000
VERSION     DAVKQB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.123"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            21..1031
                     /locus_tag="VW528_002163"
     CDS             21..1031
                     /locus_tag="VW528_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin formation protein"
                     /protein_id="HEU9912888.1"
                     /translation="MVINNIYDEYPIWSIPVNFKGSKWCLEQTGNDMYRGRAGILLFM
                     YYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSIIEDYDTNTLA
                     CLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKERVLRLVLSLG
                     DTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELILQLVPREKIKIH
                     SLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDYMKEMDTSMELES
                     LLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALNQNDKKIVIPSLL
                     EI"
BASE COUNT          456 a          151 c          226 g          449 t
ORIGIN      
        1 tagaaaatat tgcagactct ttggttatta ataatattta tgacgaatat ccaatttgga
       61 gtatacctgt taattttaaa ggttctaaat ggtgtcttga acaaacgggt aatgatatgt
      121 ataggggtag agctggaata ttactgttta tgtactatgt aaaattattg aaagataaat
      181 catcatatac tcaactgtac aataaaatgt tatctgcaat tccgccatct gtttctttat
      241 ctaagaccac atttggttta actggggata taggatattt cattgttcta tccattatag
      301 aggattacga tacaaatact ctcgcatgct taaattatat aaagagtgtt ttaactgagc
      361 ttgaaaagac tgattttgca agtgtttcaa acaagtctga ctatattaat ggtcttctac
      421 ctttaattaa tacactggta agatattata ggagaggaat agagaaggaa cgtgttctaa
      481 ggctagtatt aagtttaggt gatacgctat ataatgatgt aagcgagaga gataatagta
      541 attttggata tagttttggc catggactat caggtgtaat ttttacatta aaaaatattt
      601 ataaagtgac aagacttaaa aaatataaag aattaatttt acaactagtt cctagggaga
      661 aaatcaaaat tcattctttg aagtggtgta gtggagaatt tgggacatta gttaatcatc
      721 cccaaagtgt tccagaggga attgtagcta gtttagataa tgatacattt tgccatggaa
      781 gtatggccat aataaatttt tacatcgact acatgaaaga aatggatact agtatggaat
      841 tggagtcttt attgtgtagt gttgtaatga ataaatcaaa agaaggagag tatcgtatcg
      901 tccaaacaag caatttccca gatttttctt tgtatacagg tgtgactggt attgcttata
      961 cttttttaag gtatatcgca ctgaatcaga atgacaaaaa gatagttatc ccctcattac
     1021 ttgaaatcta gcttaattta tagttttgtt tctaaaatag aggtgtccac tcattaagca
     1081 atataaaaat accaagattg actagtttaa tatgtaattc tataaaatat ttttaatagt
     1141 gtagcttttc attgagataa taatataata aagaaaagtt ctattctaat tgctaaacgg
     1201 cttgaggtat atgctactgt tatagaaatg attttaaact aaaatcaaaa ttactccaaa
     1261 aatagtattg attttggttt aa
//
LOCUS       DAVKQB010000124         1252 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKQB010000124 DAVKQB010000000
VERSION     DAVKQB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.124"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            407..>1252
                     /locus_tag="VW528_002164"
     CDS             407..>1252
                     /locus_tag="VW528_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEU9912889.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEQWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLK
                     GWIGNL"
BASE COUNT          578 a          166 c          224 g          284 t
ORIGIN      
        1 gttaaatgat gaagaaataa gattaaacat aatggaaatt ttagaagaaa gaatagatga
       61 taatgtagaa aaatcatcta aagatgaaat aacataattg tcccttggtt acaagggcgc
      121 aattatacac cctaaagggt gcttgcgtcc tgcggagtca aacccttgca gggagcaaca
      181 accattcgct ttttaaaagt caagggtaaa taaactcgct aaggctcgcc cttgactttt
      241 aaaatttgaa atgaaagtaa caattaagcc gacatactga aacaacaaaa attaattcag
      301 tagtcggctt tttttattct atcatttcaa aacgctcatt cacaaagagg atataaaaaa
      361 actattaagc ctccacctaa aacaacaaag aaaggaagtg ataaaaatgg cagacagttt
      421 tcatttttca gtaaacataa tatcaagagg aaaaggcaaa agtgcagtag caagtgcagc
      481 atatataagt ggagaaaaaa taaaaaatga atgggacgga gtaacccatg actacacaag
      541 aaaagaaaaa atattagtaa aaaatataat attgcctgat catataccaa aagaatttaa
      601 tgataggtct accttatgga ataaagtaga aatggcagaa aaaaattcta atgcacaact
      661 agcaagacaa ttcataatag gactaccaaa agagttatct ttaagtgaaa ataaaaacct
      721 ggttgaaaga tatataaaag aaaatttaac atcacaagga atgatagtag attatgcaat
      781 tcatgatgag agtcaagata aaaatggaaa tatccattgt catataatga ccataatgcg
      841 accaataaac gaaaaaggag agttcttagc caagtcaaaa aaagaatata tcctagatga
      901 aaaaggagaa aaagtattaa acaaaaatgg aaaaccaaag acaagaaaag tagaactaac
      961 aacctggaac gatacaggca atgtagaaca atggagagaa aatttttcag atctttgtaa
     1021 caaatattta gaaagagcag gagcagaaaa aagagtagac cataggagtt ttaaaagaca
     1081 aaattcagat tatctaccga caatccattt aggatcagca gcaagtgcaa tggaaagaaa
     1141 gggaatagaa actgacaagg gaaactataa tagagaaata agaaaatata atcaacttgt
     1201 aaaaacaata aaagaagaga taaaaacatt aaagggttgg ataggaaatt ta
//
LOCUS       DAVKQB010000125         1243 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKQB010000125 DAVKQB010000000
VERSION     DAVKQB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.125"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            44..784
                     /locus_tag="VW528_002165"
     CDS             44..784
                     /locus_tag="VW528_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9912890.1"
                     /translation="MENINIKIPEEKISVIIGSNGCGKSTLIKTLSRLIKPLEGEVLL
                     DNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGRFPYRKPFKSLGKDDLEII
                     NRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSILLLDEPTTYLDISYQIEL
                     LDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKLVAEGKPEDILNDKLVKDI
                     FNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT          513 a          197 c          199 g          334 t
ORIGIN      
        1 gtggtcaaat aatcttacct gcggttatga tgaaaaaata atcttggaaa atataaatat
       61 aaaaatacct gaagaaaaaa tatcagttat tattgggtca aatggttgtg ggaaatcaac
      121 actcattaaa accttgtctc gacttataaa gccattagag ggagaagtat tgcttgataa
      181 taaatcaatt aattcttata aagaaaaaga tttagcaaaa cacatagcta tattacctca
      241 atctccaata atccctgaat caataacagt agctgatctt gtaagccgtg gtcgtttccc
      301 ctacagaaag ccttttaaga gtcttggaaa agatgacctt gaaataataa acagatcaat
      361 ggttaaggcc aatgttgaag atctagcaaa taacctagtt gaagaacttt ctgggggtca
      421 aaggcaaaga gtatggatag ctctagccct agcccaagat acaagtatcc tacttttaga
      481 tgagccaact acttacttgg atatctcata tcaaatagaa ctattagacc tcttgactga
      541 tctaaaccaa aaatataaga caaccatttg catgattttg cacgatataa atctaacagc
      601 aagatacgct gattacctat ttgcaattaa agaaggtaaa cttgttgcag agggaaagcc
      661 tgaagatata ctaaatgata aactagttaa agatatcttt aatcttgaag caaaaattat
      721 acgtgaccct atttccaatt cgcctctaat gattcctatt ggcaagcacc atgttagctc
      781 ttaataattg aatattggaa ttcatttaaa agtcgcaaat tttgaactga ccctcaaaag
      841 ttaggccaaa aaactaacta aaggaggtca gttttttaat ggcaaataca gcacagaatt
      901 taagataaaa ttagtaaaag aatatcatga atgaaaaata ggatatagag aataacagca
      961 atattaaaaa gatcaggact gataataaac aaaaagaaag tacaacgact agttcaaaag
     1021 ctaaaacttc aagtaaaaag ttattcaaga aaatctagaa aatactcatc ctacaaagga
     1081 caggtaggaa aaatatcaga caacaaaata aaaagaaata tttattaccg aaaaaaaggg
     1141 gaaatcgagt caagtaccaa caggctcatc tattccaaaa agatatttac tcgatggaaa
     1201 aactcctaga attagtgcta caaatattga caatggtatc tta
//
LOCUS       DAVKQB010000126         1240 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKQB010000126 DAVKQB010000000
VERSION     DAVKQB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.126"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..157)
                     /locus_tag="VW528_002166"
     CDS             complement(<1..157)
                     /locus_tag="VW528_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912891.1"
                     /translation="MIPKFRVWVKIGKRMVFSDDILAIDYENKEIVTQQVYFENGLPD
                     DRDIYCYD"
     gene            complement(154..222)
                     /locus_tag="VW528_002167"
                     /pseudo
     CDS             complement(154..222)
                     /locus_tag="VW528_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(270..728)
                     /locus_tag="VW528_002168"
     CDS             complement(270..728)
                     /locus_tag="VW528_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912892.1"
                     /translation="MDDILQALAKMLNMTVDEVSSLLTTFKGNAPQIYEMFVKEKMFY
                     DLFSLFQIMSIVIFSISAVVLAVLTLIYFTYDGGFVYSYDIRTGKTEEEIKLERIERK
                     RKDLKIPLKISCISSSASLITLVIAIVLKATLAPNYIFIVNEILPKLTKR"
     gene            complement(728..988)
                     /locus_tag="VW528_002169"
     CDS             complement(728..988)
                     /locus_tag="VW528_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEU9912893.1"
                     /translation="MKRFIAIWILLSAGLNIWQSIYIKKLEEKRPIVVYKADNAGAEI
                     FGKVVEKGRHGKLYTLTIRDYGVFVVTKDVYEKVKVGDEVLL"
BASE COUNT          373 a          274 c          173 g          420 t
ORIGIN      
        1 aatcataaca atagatatct ctatcgtctg gtaaaccatt ctcaaaataa acttgttgtg
       61 tcactatttc tttgttttcg tagtcaatag caagaatgtc atctgaaaaa accatacgtt
      121 ttcctatttt tacccacact ctaaatttcg gtatcatgcc aaatcctcct ctttgacaaa
      181 cgagccatca atccagcgac cttttcggtc tttgatttca cggtcgataa accattgttc
      241 gactttttct agtgcgttca tgataactcc tatctcttcg ttaattttgg taaaatctca
      301 ttcacgatga atatataatt aggtgcaaga gttgctttta aaacaattgc aataactaat
      361 gttatcaaac ttgcgcttga tgaaatgcaa ctaattttta gtggtatttt taagtccttt
      421 cttttccgtt caatgcgttc taatttaatt tcttcctcgg tttttcctgt acgtatatca
      481 taggaataaa caaaaccacc atcgtatgta aaatatatga gagttaaaac tgctaaaact
      541 actgcagaaa tactaaatat tacaattgac atgatttgaa aaagactgaa gagatcataa
      601 aacatctttt ctttaacgaa catttcataa atctgtggtg cattcccttt aaatgttgta
      661 agcaaagaac ttacttcatc aacagtcata tttagcattt ttgctaaagc ttgtaaaata
      721 tcatccatta gagtaacacc tcatccccta ctttcacttt ctcatacacg tccttcgtaa
      781 ccacgaacac cccgtagtca cgaatggtaa gcgtgtatag cttgccatgc cgtcctttct
      841 cgacgacttt accgaatatc tcagcgcctg cgttatcagc tttatagacg acaatagggc
      901 gcttttcttc taacttttta atgtagatac tctgccagat attcaatcca gcagatagca
      961 gaatccagat agctatgaat cgtttcaatt tatgacctcc tccttcaatt taagctcaat
     1021 ctctaagtaa aagctttgat caggtatctc cagtatcgct gtagtggttt taccatcaga
     1081 accaacgata atttctccga ttgccaaaac taagtctcca attgtgctat ttagcgtaag
     1141 gctcatcact ccacctcctc aatctcaaac ccttcacaat caaacaccca gccgattttt
     1201 gcattttcta gttctttacg ggtgtgggaa tatataacat
//
LOCUS       DAVKQB010000127         1187 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKQB010000127 DAVKQB010000000
VERSION     DAVKQB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.127"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..259
                     /locus_tag="VW528_002170"
     CDS             <1..259
                     /locus_tag="VW528_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912894.1"
                     /translation="STGLKDKNGKEIFEGDIVRTTRFLGRADEIGGFYEYDKEFIGIV
                     KQLEGSWVIDTGSDAVCLWTEIEENEIIGNIYENKEFGGRK"
     gene            256..627
                     /locus_tag="VW528_002171"
     CDS             256..627
                     /locus_tag="VW528_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912895.1"
                     /translation="MRPCKYPYSGRRKKQETPSPIFSARPIFDEVPIVEEVKVEFGVE
                     ASMGRIYPETLIHLDISGYGNRVHSVHRFPGILLSVGESIQLKMLFYKRLRNFTTDRF
                     LTFRESDWKFFIRDLVNEFKH"
     gene            700..1104
                     /locus_tag="VW528_002172"
     CDS             700..1104
                     /locus_tag="VW528_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU9912896.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWSEVKVQ
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKYRAVLRMTYINK
                     GTADSVCYDLNMSRTTYYRLKNEAVLALEEVI"
BASE COUNT          409 a          183 c          279 g          316 t
ORIGIN      
        1 atcaacagga ctcaaagaca agaacggcaa ggaaatcttt gaaggcgata ttgtacgaac
       61 tactagattt ttgggtagag ctgacgaaat tggcggtttc tatgagtatg acaaggaatt
      121 tatagggatt gttaagcagc ttgagggttc ttgggtaatt gatacgggca gtgacgcagt
      181 atgtttatgg actgaaattg aagaaaatga aatcatcggc aacatctatg aaaataagga
      241 gtttggagga cgcaaatgag accttgtaaa tatccatatt caggaagaag aaaaaaacaa
      301 gaaacgccgt cgccaatatt ttctgcacga ccaatttttg acgaagttcc aattgtagaa
      361 gaagttaagg ttgagttcgg agttgaagct agtatggggc gcatatatcc agaaacgtta
      421 atacatttag atatttctgg atacggaaat agagtgcatt cagtacatcg cttccccggt
      481 attttactga gtgttggtga gtcaatccaa ctaaagatgc ttttctataa aagacttaga
      541 aattttacta cagatcgttt cttgacgttt agagaatctg attggaagtt ctttatccgg
      601 gacctggtca acgaatttaa gcattaaaaa aagccaagac actctctgcc tcagctaata
      661 gttctcgcaa agactattat atcacaaagg agacagagag tgaacaaggc taaagagctc
      721 ttgaaagagt tgcaggatct ggacatggac atccaaagcc gtatagacga aattaacgag
      781 cttgaggcag gtttgctctc aagccctaaa tggtccgagg ttaaagtcca aggtggacag
      841 actagaaaag ttgatgatgt ctatactcag ttggtagtga tgaaagaggc tatagagcag
      901 gatactaaag aggtcattaa cagaaaactt gaattaggta gaatgatcaa taggcttaaa
      961 aatccaaagt acagggcagt attaagaatg acttacatca acaaaggcac cgctgatagc
     1021 gtttgttatg atttgaatat gagtcgtaca acctactaca ggttaaaaaa tgaggcggtc
     1081 ttagctttgg aagaagttat ctaacctcat agtgagctta tgggactttt tggaacagca
     1141 cggttctaaa aatctgttag aatggtagta tcaagaattg aaaagag
//
LOCUS       DAVKQB010000128         1150 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKQB010000128 DAVKQB010000000
VERSION     DAVKQB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.128"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            145..>1150
                     /locus_tag="VW528_002173"
     rRNA            145..>1150
                     /locus_tag="VW528_002173"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          325 a          229 c          350 g          246 t
ORIGIN      
        1 tcgcttgaga gaagcaagtg acaaagacct ttgaaaactg aacaagacga accaatgtgc
       61 agggcgctac aacataagtt gtagtactga acaatgaaaa aacaataaat ctgtcagtga
      121 cagaaatgag tgagaactca aacttttaat gagagtttga tcctggctca ggacgaacgc
      181 tggcggcgtg cctaatacat gcaagtagaa cgctgaagga ggagcttgct tctctggatg
      241 agttgcgaac gggtgagtaa cgcgtaggta acctgcctgg tagcggggga taactattgg
      301 aaacgatagc taataccgca taagagtgga tgttgcatga catttgctta aaaggtgcac
      361 ttgcatcact accagatgga cctgcgttgt attagctagt tggtggggta agggctcacc
      421 aaggcgacga tacatagccg acctgagagg gtgatcggcc acactgggac tgagacacgg
      481 cccagactcc tacgggaggc agcagtaggg aatcttcggc aatggacgga agtctgaccg
      541 agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa gctctgttgt aagagaagaa
      601 cgagtgtgag agtggaaagt tcacactgtg acggtatctt accagaaagg gacggctaac
      661 tacgtgccag cagccgcggt aatacgtagg tcccgagcgt tgtccggatt tattgggcgt
      721 aaagcgagcg caggcggtta gataagtctg aagttaaagg ctgtggctta accatagtag
      781 gctttggaaa ctgtttaact tgagtgcaag aggggagagt ggaattccat gtgtagcggt
      841 gaaatgcgta gatatatgga ggaacaccgg tggcgaaagc ggctctctgg cttgtaactg
      901 acgctgaggc tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgctg
      961 taaacgatga gtgctaggtg ttagaccctt tccggggttt agtgccgtag ctaacgcatt
     1021 aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc
     1081 cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct
     1141 tgacatccct
//
LOCUS       DAVKQB010000129         1134 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKQB010000129 DAVKQB010000000
VERSION     DAVKQB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.129"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..522
                     /locus_tag="VW528_002174"
     CDS             <1..522
                     /locus_tag="VW528_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU9912897.1"
                     /translation="QEINKLREVREINLGGGFMKLFMENRLKEFFLSLMEIYKKYDID
                     STVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVITLDTSIYTNTLWFVPHIITTLN
                     SSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSIVFFPVGAYIKSNHSNLHRNDF
                     MREVYLWTKNLTY"
     gene            498..>1134
                     /locus_tag="VW528_002175"
     CDS             498..>1134
                     /locus_tag="VW528_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912898.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDLINNENYLFEYACRIREEWRKKCFNHSYRFLCSPIITDDFLN
                     TKTLRSSQIEYKYERYLSKSSIGDRAVDGFVSFNTLTANGMSAIKLCLEILNSIFFKK
                     KIDLLYSTGYYETRFLLNNLAKSGISCYEVSNCELDKDKFYNVFMMEPNRAD"
BASE COUNT          417 a          135 c          194 g          388 t
ORIGIN      
        1 caagaaatta ataaacttag agaggttaga gaaataaatc ttggaggcgg ttttatgaaa
       61 ttatttatgg aaaatcgttt gaaagaattt tttctatcac ttatggaaat ctataaaaag
      121 tacgatattg atagtactgt gactacaata atagaaccag gtagtgcaat tacttcattt
      181 tctgcctata tgattactag cccagttaat gttagtgagg tgaatgagca gcaggttatc
      241 acgttagaca catcaatata caccaataca ttatggtttg ttccgcatat tattacaacg
      301 ttaaattcaa gtagtaaaga gcgttatagt actattctct atggtaatac ctgttatgaa
      361 catgacaagt ataaaatgaa agtttcgctt ccaaggttaa ctcaaaatag cagtatagtg
      421 ttttttcctg taggagctta tataaaaagc aatcattcaa atttacatcg taatgatttt
      481 atgcgggagg tatatttgtg gacaaaaaac ttgacatatt agataaagtt aaggaatatt
      541 taggaaataa aactactcaa attctggata atcaatataa agaatttttg aaacttaatg
      601 atataaggcg agcgtttggt atttcagaaa aagtattaaa caattctttt aattttacga
      661 gtaaagaatt taatgattta attaataacg aaaattattt attcgaatat gcatgtagaa
      721 ttagagagga atggagaaaa aaatgcttta atcattctta tcgttttcta tgctcaccta
      781 taattacaga tgattttctt aacacgaaga cattgagaag tagccaaatt gaatataaat
      841 atgagcgata tttatcgaaa agttcgatag gcgatagagc ggttgatggc tttgtttcct
      901 tcaatacttt aacagctaat ggtatgtctg ctattaaact atgtcttgag atattaaact
      961 ctattttctt caagaagaag attgatttat tatattcaac cggatattat gaaacaagat
     1021 ttttattaaa taatcttgct aaatcaggta ttagttgcta tgaggtaagt aattgtgaat
     1081 tggataaaga taaattttat aatgtattca tgatggaacc caatcgagcc gatt
//
LOCUS       DAVKQB010000130         1106 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKQB010000130 DAVKQB010000000
VERSION     DAVKQB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.130"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..398
                     /locus_tag="VW528_002176"
     CDS             <1..398
                     /locus_tag="VW528_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEU9912899.1"
                     /translation="KVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIADD
                     LVTATVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRKA
                     IDSTCSMLQPQSLVKELIKLENIVLEEGH"
     gene            619..>1106
                     /locus_tag="VW528_002177"
     CDS             619..>1106
                     /locus_tag="VW528_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU9912900.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WT"
BASE COUNT          397 a          140 c          198 g          371 t
ORIGIN      
        1 ataaagttta tgatgaaata tttgttgaag aggtagttcc gcacgttttt ctgccatatg
       61 aatctgactt acttcttatt ttaccagcta cggcaaatgt gattggcaaa attgctaatg
      121 gtattgctga tgatttagtt acagcaactg ttttaaactt taataaaaaa ataatttttt
      181 gtcccaatat gaactctact atgtgggaca atcacatagt tcaaagaaat gtatcaattc
      241 taaaggagtt gggacatata tttttatttg agtctaaaaa aacatatgag gtaggattgc
      301 gtaaagcaat agattcaaca tgttcaatgt tacaaccaca gtcgttagta aaagaactta
      361 tcaaattaga aaatattgtc cttgaagagg gacattaaaa actactgaga atattaagga
      421 ggggaaaaaa tggaaaattc atcaatcgat gtagatatgc tgttggaaga attgacacaa
      481 gaagcaatgg tcgttgttgc tgttgataag gactgttaat ttaaacttat ggcaatatat
      541 gaaaggttac tggatgtttt aaattatgca ggcagtagcc ttttattata tacaaatgga
      601 taaagtaagg ataatacaat gattaataaa aaaatacaac aagttgtttt ggaatcatta
      661 cagaattttt tgaatgggaa cttcatttcg ccttgtgtag tctatgattt tggcttgctg
      721 gaaactgtac ttgatgaatt taaaaatcaa attcctgtaa cattcaatta ccaacttttt
      781 tatgccgtta aagcaaattc aaatgagaag atacttgaat tcttagtaga taaaattgat
      841 ggagttgatg tggcgtcatt atctgaatta gatgtggcta aaaaattttt cccaccaact
      901 caaatttctg ttaatggtcc cgcattttct tatgaaactt tatataatct gattaaaaaa
      961 caatataaag ttgatattaa ctttttggaa catcttcaac aattttcccc aaaagaatct
     1021 gttggaataa gagtaacgga gccagatgaa cttaataatc gtatgagtcg atttggaata
     1081 aatatttgca gtgataattg gactag
//
LOCUS       DAVKQB010000131          986 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKQB010000131 DAVKQB010000000
VERSION     DAVKQB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.131"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>986
                     /locus_tag="VW528_002178"
     CDS             <1..>986
                     /locus_tag="VW528_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9912901.1"
                     /translation="KLLDIKDPNIQILDIIKKDTHKEIIAKLDYDAPSCPECGNQLKK
                     YDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQIPRIINQKI
                     AQKLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSCLPEIMSWDEYAFTKGKMSF
                     IAQDFNNLNIITVLKGRTQAVIRNHFLKYDRAVRCRVKIITMDMFSPYYDLARQLFPC
                     AKIVLDRFHIVQHLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSDKRF
                     YRPTFRMHLTNKEILDKLLSYSQDLKHHYQLYQLLLFHFQNKKPEKFFGLIE"
BASE COUNT          317 a          210 c          163 g          296 t
ORIGIN      
        1 aaaattacta gacattaaag accctaatat ccagatttta gacatcatca agaaggatac
       61 acacaaggaa atcatcgcca aactggacta cgacgcccca tcttgccctg agtgcggaaa
      121 ccaattgaag aaatatgact ttcaaaaacc ttctaaaatt ccttatcttg aaacgactgg
      181 tatgcctact agaattctcc ttagaaagcg tcgattcaag tgctatcact gttcaaaaat
      241 gatggtcgct gaaacttcta tcgtcaagaa gaatcaccaa atccctcgta tcatcaacca
      301 aaagattgct caaaagttaa ttgaaaagat ttctatgact gatattgccc atcagctttc
      361 catctcaact tcaactgtta ttcgtaagct caatgacttt cactttaaac atgatttttc
      421 ttgtcttcct gagattatgt cttgggatga gtatgctttt acaaaaggaa agatgagctt
      481 cattgcgcaa gattttaaca atctcaatat catcactgtt cttaagggaa gaacacaagc
      541 tgtcatccga aatcactttc ttaaatatga tagagccgtc cgatgtcgcg tcaaaatcat
      601 tactatggat atgtttagtc cttactatga tttagctaga caacttttcc cgtgtgctaa
      661 aatcgtgttg gatcgctttc acattgtaca acatcttagc cgtgctatga gtcgtgtgcg
      721 tgttcaaatc atgaatcagt ttcatcgaaa atcccatgaa tacaaggcta tcaagcgcta
      781 ctggaagctc atacaacagg atagccgtaa actgagtgat aagcgatttt atcgtcctac
      841 ttttcgtatg cacttaacca ataaagagat tttagacaag cttttgagct attcacaaga
      901 cttgaaacac cactatcagc tctatcaact cttgctgttt cactttcaga ataagaaacc
      961 ggagaaattt ttcggactca ttgagg
//
LOCUS       DAVKQB010000132          971 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKQB010000132 DAVKQB010000000
VERSION     DAVKQB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.132"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            97..552
                     /locus_tag="VW528_002179"
     CDS             97..552
                     /locus_tag="VW528_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912902.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            549..695
                     /locus_tag="VW528_002180"
     CDS             549..695
                     /locus_tag="VW528_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912903.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
     gene            832..971
                     /locus_tag="VW528_002181"
                     /pseudo
     CDS             832..971
                     /locus_tag="VW528_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          351 a          142 c          189 g          289 t
ORIGIN      
        1 tttttatgtg cagagttata gtggcttgat gctgggaaag tacactgtga ctgctaaaac
       61 attcctagaa gttgctttga tttacctaat ctatttatgc aaattttatg ttattttact
      121 ataacagttg ttgctaagcc aaataatagt ggggaagttc atttagacgt aagcattgaa
      181 gataatcagg gaggtagtgg gcataatttc agttctgttt caagtagctc acaaacagct
      241 aaatatgaag aaactggtta taataacaat tcatcattat atataacgat tgataaaacg
      301 tctgatgcaa cagctctttt gaaattaaag ttgaataatg ttgataatca acctgctact
      361 gaagttccta gttcaggaat tactgtaaaa ttaaatgcta aagataatgc tggaaactgg
      421 acaagtgctt cgaataaaaa agaaataaca gtaaaaattg tttctgctaa accgacatat
      481 ccagacaaaa tcttagtgaa aaatcctgat aatataaaag atacagaaaa aatgccatta
      541 ttgaaaaatt gaaagaggca aataaaaatc atccagcagg agctccaacc tttgctaaag
      601 gtgaaggaga gcatgcaaat gatattgtag caacttattc agatggtaca acttattatg
      661 taccgttaaa tgatgtgaca aaatatgcga ggtagtggct gtaccactca cttattcacc
      721 tccccgtgat ttgtagtagt gataggcttt ctcactatta ttataaaaca aaataaagat
      781 cacaacactt tttcattctg tgttgtgcct tgagtgaaac gaaaggaatg aattataaat
      841 atgaaaagta tagtcactag catagatgag cgcttgcgta ctcgcctacg agtgattatc
      901 tggaagcaat ggaagaagaa atcgagacga ttatggggat tgcttaagtt aggggttcct
      961 aaatggatag c
//
LOCUS       DAVKQB010000133          922 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKQB010000133 DAVKQB010000000
VERSION     DAVKQB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.133"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(87..836)
                     /locus_tag="VW528_002182"
     CDS             complement(87..836)
                     /locus_tag="VW528_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU9912904.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
BASE COUNT          318 a          183 c          104 g          317 t
ORIGIN      
        1 actttatatt tagatgcaaa tagtaacgcg taggccagcc caacatgccc caaaccaatt
       61 actgctatat tcataaaact acttccttat ttcttaatcc aaaatctaat agaataagct
      121 gccccattcc ttaaatacaa ctctttaata ttgtttaaaa gtttttcaac tgatttccag
      181 attatcaaaa tctgagattt atagcacaat attgatgata ttctatcaat ataatgtttt
      241 tcatcaagtt cctcttgata catttttaat tctttagttt ttcccatata actaaccata
      301 ctactatcac ttacatatgg gaagtcctca taatatatta ctttataacg cataaattca
      361 agcgcccttc caatactatt cacaaaaaca tgatcaacat ggtcaccaag tgaaagcgga
      421 caatatacga cacatttatc gtctaaatgc attaacagct cttttatgat atcattcttt
      481 aatgtgtcct cattttttaa ttcactatag atatgacggt atagaaaatt gccatttcta
      541 tctttcctat agagacattc atagtacgat aagtgtctaa aatcacattg tagacgttca
      601 caagctaacc tgtcttcttt cttcctttct tcaatcggat atttcccaag gttacacaac
      661 ttatgaaatt gcttagcaga gggctgtagc tgttggctca aagggtaacc agaaaatata
      721 gtaataacaa gtacaatttc tccttctgaa gttaattttg aaatataatc accacaggaa
      781 aaaattgcgt catctaaatg tggagataaa aagatatact tagtattgtt actcataacc
      841 attccctcta caatttatct aaaaactcac taagtgtctg attaaattcc acatcatcaa
      901 aaaaattcac cttattctta at
//
LOCUS       DAVKQB010000134          918 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKQB010000134 DAVKQB010000000
VERSION     DAVKQB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.134"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            87..269
                     /locus_tag="VW528_002183"
     CDS             87..269
                     /locus_tag="VW528_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU9912905.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            279..644
                     /locus_tag="VW528_002184"
     CDS             279..644
                     /locus_tag="VW528_002184"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU9912906.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            641..>918
                     /locus_tag="VW528_002185"
     CDS             641..>918
                     /locus_tag="VW528_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9912907.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRL"
BASE COUNT          379 a          116 c          152 g          271 t
ORIGIN      
        1 gaccgaatta gaaagaaaaa atcgaaaaat tagctaagaa atattctgat aacttaaaca
       61 tcaaagttca agagagagtt cgtgaaatgg caaatgataa taagagccat tatttgatat
      121 acagagtttt aggtatttca tttgaagaag gagaaaatat cgatttgtat caaaataaag
      181 gtcgtttttt atacaaatat gctggttcat ttttagaaga agctgcagta ctatccttta
      241 acgaaaaatt tggtacagaa aatacttaaa aagttaacat tcctaattct gaaagtacaa
      301 aacctaagac ttttgaaatt gattgtttag tcggagaaaa acacgcatac gaaataaaat
      361 ggtgggatgc aactacagat ggagaccata taactaaaga acacactaga ataaaagtta
      421 ttcataacaa aggatatata ccaattcggt taatgttcta ctatccaaat agaactcaag
      481 ctataaaaat tcagcaaact ttagaaacat tgtataacgg tattggaggg aaatattatt
      541 atggagattc tgcctgggaa catttaagag cagtgaccgg tattgattta cttagtattc
      601 taacagatat tgcaaataaa aaaacagggg taaaatcaaa atgacagtat taaaaggaga
      661 taacttagaa atattaaaaa ctattgaatc ctcaagtatt gatttaatct atatggaccc
      721 tcctttcttt acacagaaaa cccaaaaatt atctaataac aaaaatatta tgtattcatt
      781 cgaagatacg tggacttcga ttgaggatta caaagaattt ttgtctgtaa gattagaaga
      841 atgcaaaaga gtgctaaaaa atagtggcag tattttcgtt cattgtgata aaattgcaaa
      901 tcatcatatt agattaat
//
LOCUS       DAVKQB010000135          861 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKQB010000135 DAVKQB010000000
VERSION     DAVKQB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.135"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..497
                     /locus_tag="VW528_002186"
     rRNA            <1..497
                     /locus_tag="VW528_002186"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            549..621
                     /locus_tag="VW528_002187"
     tRNA            549..621
                     /locus_tag="VW528_002187"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:582..584,aa:Ala,seq:tgc)
     gene            746..>861
                     /locus_tag="VW528_002188"
     rRNA            746..>861
                     /locus_tag="VW528_002188"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          227 a          189 c          248 g          197 t
ORIGIN      
        1 caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg
       61 agcgcaaccc ctattgttag ttgccatcat ttagttgggc actctagcga gactgccggt
      121 aataaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg acctgggcta
      181 cacacgtgct acaatggctg gtacaacgag tcgcaagccg gtgacggcaa gctaatctct
      241 taaagccagt ctcagttcgg attgtaggct gcaactcgcc tacatgaagt cggaatcgct
      301 agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg ggccttgtac acaccgcccg
      361 tcacaccacg agagtttgta acacccgaag tcggtgaggt aaccgtaagg agccagccgc
      421 ctaaggtggg atagatgatt ggggtgaagt cgtaacaagg tagccgtatc ggaaggtgcg
      481 gctggatcac ctcctttcta aggataagga actgcgcatt ggtcttgttt agtcttgaga
      541 ggtcttgtgg ggccttagct cagctgggag agcgcctgct ttgcacgcag gaggtcagcg
      601 gttcgatccc gctaggctcc attggtgaga gatcaccaag taatgcacat tgaaaattga
      661 atatctatat caaatagtaa caagaaaata aaccgaaaac gctgtagtat taataagagt
      721 ttatgactga aaggtcagaa aataaggtta agttaataag ggcgcacggt ggatgccttg
      781 gcactaggag ccgacgaagg acgtgacaaa cgacgatatg ccttgggtag ctgtaagtaa
      841 gcgatgatcc agggatttcc g
//
LOCUS       DAVKQB010000136          857 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKQB010000136 DAVKQB010000000
VERSION     DAVKQB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.136"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(95..283)
                     /gene="pneA2"
                     /locus_tag="VW528_002189"
     CDS             complement(95..283)
                     /gene="pneA2"
                     /locus_tag="VW528_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEU9912908.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
     gene            complement(290..514)
                     /gene="pneA1"
                     /locus_tag="VW528_002190"
     CDS             complement(290..514)
                     /gene="pneA1"
                     /locus_tag="VW528_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEU9912909.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
BASE COUNT          277 a          166 c          106 g          308 t
ORIGIN      
        1 gacttactat tgttttaaaa aaaaacctct ctcaaaatta ttagttacga gagaggaaaa
       61 taaagagcaa cttttatttt taaaattgaa tattttattt tttcttgttg tctttgccac
      121 aacgagtctg agctgacccc aaatatgatg caataaaact taatgtagca gaacatataa
      181 ttgttgatct agggtcagca ccatctgtgc caccataaac caattgcatt tcttcaagag
      241 ataattcttt caaactcttt ccgattacaa aatcattttt cattttaatt taccccaaac
      301 aatcattatt gtagctaact agccccccaa taccagaaac agctgcacta atgcatactt
      361 tcgagctagc tgcagctgat ttcaaaataa taggagtagg cgtccaacgt ggctctgcac
      421 catcagatgc accataaatt aaactcattt catcagcaga caatgatttt aaactttttc
      481 cacaaaattt ttcgtttgaa ttaaagttgg tcataagtaa taactcctcc ttataaaaag
      541 ttaaataagt tgctctatat ttttcgtttt ttcagataaa ttataattct ctcattctcg
      601 tgcaaaatat cgggttttaa tccgccttcc caacatcaag accattggac ttcttttgcg
      661 attgcactcg aatctccaat tgtttattct tctgaataaa ctgttggtaa ggaattgtat
      721 tactaaacat aaaaacacct agcaatgcaa atgtaactaa ttttttaatt ccacgttttt
      781 ttctcatgca atgaaacccc tttctttttc cttgttttta ttctaacaga tatattttat
      841 ttagtcaagt attttta
//
LOCUS       DAVKQB010000137          838 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKQB010000137 DAVKQB010000000
VERSION     DAVKQB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.137"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..792
                     /locus_tag="VW528_002191"
     CDS             <1..792
                     /locus_tag="VW528_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY-like element IS1202 family transposase"
                     /protein_id="HEU9912910.1"
                     /translation="FDKQETLNAYYHVLEQILANHGIPLQMKTDKRTVFTYQASNSKK
                     MEDDTYTQFGYACHQLGILLETTSIPQAKGRVERLNQTLQSRLPIELERNKIHTLEEA
                     NTFLLSYIQTFNEQFGNKTKLSVFEEAPNPSERNLILARLAERVVDSGHHIRFQNRCY
                     IPTEQGKEVYFIRKTKALVLKAFDGDIYLNIADKIYHTKELLDHELYSKNFEQEPEQK
                     KERRKYIPPQTHPWKLTSFKQYLHKNKKDYEEFTSEEIHSPQLQV"
BASE COUNT          299 a          200 c          136 g          203 t
ORIGIN      
        1 tttgacaaac aagagacctt gaatgcctac taccatgtcc tcgaacaaat cttagccaat
       61 cacggcattc cccttcaaat gaaaactgat aagagaacgg tctttaccta tcaagcatcc
      121 aactctaaga aaatggagga cgacacctat acacaatttg gatacgcctg tcaccaacta
      181 gggattctcc ttgagaccac ctctatccct caagctaaag ggagggtcga aagactcaat
      241 cagacactac aatcacgcct gcctatcgaa ctggaacgaa acaagattca tacattggaa
      301 gaagccaata ctttccttct ttcctacatc caaaccttta atgaacagtt tggaaataag
      361 acaaaactct ctgtttttga ggaggctcct aacccctctg aacggaatct cattctagct
      421 agactggcgg agagagtcgt cgatagtgga caccatatcc gattccaaaa ccgttgctat
      481 attcctaccg aacaaggaaa agaagtctat ttcatcagaa agacgaaagc attagttcta
      541 aaagcatttg atggtgacat ctacctcaat atcgctgaca aaatctatca cacaaaggag
      601 ctactagatc atgaactcta ttcgaaaaac tttgaacaag aaccagaaca aaaaaaagaa
      661 agacgcaagt atatccctcc acaaacccat ccgtggaaac tcacatcttt caaacaatac
      721 cttcataaaa acaaaaagga ttatgaagag tttactagtg aggagattca ttctcctcaa
      781 ctacaagtat aatctatttt tcgctaaatg ggaagctttt ccttaaagga agtcactc
//
LOCUS       DAVKQB010000138          806 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKQB010000138 DAVKQB010000000
VERSION     DAVKQB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.138"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>806
                     /gene="phtD"
                     /locus_tag="VW528_002192"
     CDS             <1..>806
                     /gene="phtD"
                     /locus_tag="VW528_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU9912911.1"
                     /translation="YTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHS
                     HWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPL
                     DRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYV
                     EHPNERPHSDNGFGNASDHVQRNKNGQADTNQTEKPNEEKPQTEKPEEDKEHDEVSEP
                     THPESDEKENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSV"
BASE COUNT          285 a          160 c          180 g          181 t
ORIGIN      
        1 tacactgatg atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt
       61 tatatctttg atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat
      121 atgacccata gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc
      181 caggcttatg ctaaagagaa aggtttgacc cctccttcga cagaccatca ggattcagga
      241 aatactgagg caaaaggagc agaagctatc tacaaccgcg tgaaagcagc taagaaggtg
      301 ccacttgatc gtatgcctta caatcttcaa tatactgtag aagtcaaaaa cggtagttta
      361 atcatacctc attatgacca ttaccataac atcaaatttg agtggtttga cgaaggcctt
      421 tatgaggcac ctaaggggta tactcttgag gatcttttgg cgactgtcaa gtactatgtc
      481 gaacatccaa acgaacgtcc gcattcagat aatggttttg gtaacgctag cgaccatgtt
      541 caaagaaaca aaaatggtca agctgatacc aatcaaacgg aaaaaccaaa cgaggagaaa
      601 cctcagacag aaaaacctga ggaagataag gaacatgatg aagtaagtga gccaactcac
      661 cctgaatctg atgaaaaaga gaatcacgct ggtttaaatc cttcagcaga taatctttat
      721 aaaccaagca ctgatacgga agagacagag gaagaagctg aagatactac agatgaggct
      781 gaaattcctc aagtagagca ttctgt
//
LOCUS       DAVKQB010000139          794 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKQB010000139 DAVKQB010000000
VERSION     DAVKQB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.139"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            19..>794
                     /locus_tag="VW528_002193"
     CDS             19..>794
                     /locus_tag="VW528_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEU9912912.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYA
                     FTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYD
                     LAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFERKS"
BASE COUNT          251 a          166 c          131 g          246 t
ORIGIN      
        1 atcattagaa agattcatat ggaacaatta cattttatca caaaactgct cgatattaaa
       61 gacccaaaca tcaagattct agatatcatc aatatggata cccacaaaga aattatcgct
      121 aagctggatt atgaggctcc atcttgccct gattgtggaa gtctaatgaa gaaatatgac
      181 tttcaaaaac cgtctaagat cccttacctc gaaacaactg gtatgccttc tagaattctc
      241 cttagaaaac gccgtttcaa gtgctatcac tgttcaaaaa tgatggttgc tgagactccc
      301 ctggtaaaga aaaatcacca aatccctcgt atcatcaacc aaaaaattgc tcaaaagtta
      361 attgaaaaaa tttctatgac tgatattgcc catcagctgg ccatttcaac ttcaactgtc
      421 attcgcaagc tcaatgactt tcactttgag catgattttt ctcggcttcc aaagattatg
      481 tcttgggatg agtatgcctt cactaaggga aagatgagtt tcattgcgca agattttgat
      541 aatcttaata ttatcactgt tcttgaaggc agaacacaag ctgtcatccg aaatcacttt
      601 cttcgctacg atagagccgt tcgttgtcaa gtgaaaatca ttacgatgga tatgtttagt
      661 ccttactatg acttggctaa acagcttttt ccgtgtgcta aaatcgttct agatcgtttc
      721 catattatcc aacatctcag ccgtgccatg agtcgttttc gtgttcaaat tatgaatcag
      781 tttgaacgaa aatc
//
LOCUS       DAVKQB010000140          742 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKQB010000140 DAVKQB010000000
VERSION     DAVKQB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.140"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            288..587
                     /locus_tag="VW528_002194"
     CDS             288..587
                     /locus_tag="VW528_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9912913.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          280 a          135 c          126 g          201 t
ORIGIN      
        1 atagatgtaa catgtttgca aacaaagcat acgaaccttt agtaaaatca tttccatgaa
       61 actagaatag agccctctta gcaaaaatca ttattttaat ttatttctaa tcactccttg
      121 acataaataa ctctcaccaa taaaagacta tgtcttaaaa aaatggtata ataaaatcaa
      181 tacttgggct tgatggctat gctactaata acaattagga gagaaaatca ggcacttgtt
      241 aacaacaagg attatcccct tgagatgaaa ggaactttag aaatcttatg atgaacatgc
      301 aaaacatgat gcgtcaagca caaaaacttc aaaaacaaat ggaacaaagc caagctgaac
      361 ttgctgctat gcaatttgtt ggcaaatctg ctcaagatct tgtccaagcg accttaactg
      421 gcgataagaa agttgtcagc attgatttca atccagctgt cgttgaccca gaggaccttg
      481 agactctttc tgatatgacc gttcaagcca tcaactctgc tcttgaacaa atcgatgaaa
      541 ctaccaagaa aaaactgggt gctttcgctg ggaaattacc tttctaaaaa caaggagcta
      601 gaacaatgct tgtcgataac aaaggctaag aaaggtgcaa aaatgactct ataatatttg
      661 tagtgggtaa atccactata gatattatgg agcctatttt attgtagaaa aaaagtccca
      721 taagatctat aatgaaaagc ga
//
LOCUS       DAVKQB010000141          731 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKQB010000141 DAVKQB010000000
VERSION     DAVKQB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.141"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(<1..>731)
                     /locus_tag="VW528_002195"
     CDS             complement(<1..>731)
                     /locus_tag="VW528_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEU9912914.1"
                     /translation="SDIHFENLISYGDMPVIIDFETMLQQPLFDDKTGQSLLDTLFHR
                     VTRTLLLPTEGVKREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKFDIGKIDFEG
                     GKNLPVRDGDIEFDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLYGLKTRVLFR
                     DTNSYASVLSFLYHPDFYEEMLDREKALENLWSNKFSNQGIVASECEQMRLLDIPIFY
                     TDTNINEIYDDFGNHFISLHQFSGY"
BASE COUNT          249 a          144 c           81 g          257 t
ORIGIN      
        1 tcatatccag aaaattgatg tagggaaata aaatgatttc caaaatcatc gtagatttca
       61 ttgatatttg tatctgtata aaaaatcgga atatctaata acctcatttg ttcacattcg
      121 cttgctacaa taccttgatt agaaaactta ttgctccaga gattttccaa tgctttttct
      181 ctatctaaca tttcttcata aaaatcagga tgatataaaa aagatagtac tgaagcatag
      241 ctatttgtgt ctctaaaaag tacccttgtc tttaaaccat acaagtttgc ttttaatagc
      301 attttaaatt cttctgtttt atttaactct tcaaatatca gataaaaatc cctaaaacct
      361 tttttgaaat cttttatata cttatcaaat tctatatcac catcccgaac aggcaggttt
      421 ttcccacctt caaaatcaat tttcccaata tcaaacttta ccttatcagt atttaaatta
      481 attaaaactt gaccattaaa agcatccttc ttaaaatttc ctgtaagagc actcatctca
      541 acatcaagac catcctccct ttttactccc tctgttggaa gaagcaaagt tcgagtcacc
      601 ctgtgaaaca gggtatccag caaactctga ccagttttat catcaaatag aggttgctgt
      661 aacattgttt caaagtctat tattacaggc atatctccat aggaaattaa attttcaaaa
      721 tgtatatcac t
//
LOCUS       DAVKQB010000142          684 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKQB010000142 DAVKQB010000000
VERSION     DAVKQB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.142"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            complement(102..176)
                     /locus_tag="VW528_002196"
                     /pseudo
     CDS             complement(102..176)
                     /locus_tag="VW528_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            190..552
                     /locus_tag="VW528_002197"
     CDS             190..552
                     /locus_tag="VW528_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU9912915.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFGNE"
BASE COUNT          212 a          103 c          120 g          249 t
ORIGIN      
        1 ctgacttcgt cagttctatc cacaacctca aaacagtgtt ttgatctgac tacgtcagtt
       61 ctatccacaa cctcaaaaca gtgttttgat ctgactacgt cagttccatc tacaacctca
      121 aaacagtgtt ttgagcaacc tgaggctagt ttcctagttt gctctttggt tttcattgag
      181 tataacagat tgttagaagt tggtttaaat ttcctaatca gtttgttcac atttaccttc
      241 gatatattat atcccatagt taaggttggt catacagatg attatagtca tggagccgta
      301 aaacttagtg tttctttagt tgacaaagat gccatgaaaa aaatatctgt aactgtaata
      361 ggatattttg aaataaatat agatgaaaat atcaccgata ttctatacgt aaatggtact
      421 gctattcttt atcattattt acgttctatt gtttcaatag tttcggcaat tgatagcagt
      481 gaagcaatgt tgctacctac cattaatgtt ttagagttac tagataaatc tcaacctttt
      541 gggaatgagt agatggattg aataaaattt tattaagagt ggatgattta tctgtagatt
      601 attattggac agttagtctt gaagtagtct aagaattagg ttataatcag tagaatcctt
      661 gctaataatg aggaggttag ttta
//
LOCUS       DAVKQB010000143          659 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKQB010000143 DAVKQB010000000
VERSION     DAVKQB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.143"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>659
                     /locus_tag="VW528_002198"
     CDS             <1..>659
                     /locus_tag="VW528_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9912916.1"
                     /translation="LKKTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQI
                     KNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKG
                     EKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIE
                     RHPMSLSGGQKQRLAIASVMCKNSPFVFFDEPSSGMDYSNMIKISELINKYKTMDKII
                     F"
BASE COUNT          268 a           72 c          121 g          198 t
ORIGIN      
        1 gcttaaaaaa acttatacta gaagtgaatt tttaaagcta gataaaaatg aattaaatgc
       61 tttaagttta agagataaag aattaagtaa attaaaagtt ccttatttaa aagaaggtgg
      121 agagtatcag ataaaaaatc ttagctacaa atttactgat gatgagtgtt taagcttaaa
      181 agatatttcg ttcaagcttg ggaaaattta tggcataata ggatccaacg gacgaggaaa
      241 atcaacgctt ttaagatgtt taataggtct tgagaaaaaa tcaaaagaag aaatttattt
      301 taagggagag aagctatcta aaaaagaaag actcaaaaac tcttcacttg ttatgcaaga
      361 tgtaaatcat caattattca cagatgaagt attcaacgag cttagattag gagtaaagaa
      421 ttttgatgaa gaaaaggcga aaatcatttt aaaagattta ggcctggacg aatttattga
      481 aaggcatccg atgagtttat caggagggca aaagcaaagg cttgcaatag catctgttat
      541 gtgcaagaat tctccatttg tcttttttga cgaaccttca agtggtatgg attattccaa
      601 tatgataaaa atatctgaac tgattaataa gtataaaacc atggataaaa taattttta
//
LOCUS       DAVKQB010000144          640 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKQB010000144 DAVKQB010000000
VERSION     DAVKQB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.144"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>640
                     /locus_tag="VW528_002199"
     CDS             <1..>640
                     /locus_tag="VW528_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9912917.1"
                     /translation="LNQKLSQAEFEVRAGSIRQEILNATKDKASKSELTQTAEELASR
                     IASVQASGRNLFLNSLFKQDIPKTGIWTTSTYTATIDSESKYLGHKALKIIGLNPSGR
                     DGGNPKVTYPALGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVT
                     LSTEIKRYVVHIPKGWTNESKQTTNEWLFNFNQEGTVWIWMPKFEISDVDTSY"
BASE COUNT          214 a          114 c          149 g          163 t
ORIGIN      
        1 ctaaatcaga agttgagtca ggctgaattt gaggtgcgag ctggctctat ccgtcaggaa
       61 atcttgaacg caaccaagga taaagccagc aagtcagaac tcacgcagac agctgaggag
      121 ctggctagtc ggattgcgag tgtgcaggca tccggtcgaa atctattctt gaactcacta
      181 ttcaagcagg atattccaaa aacaggaatt tggacaacga gtacatatac ggctactatc
      241 gatagcgaaa gtaagtatct tggacacaag gctcttaaaa ttataggtct gaatccatct
      301 ggtcgtgatg gagggaatcc caaggttact tatccagctc tgggtcaatt cgggaaagta
      361 attcccggaa gtacgactaa tcaagatgta accattagtt tttatgctaa ggcaaataaa
      421 aatggaataa tgctaagatc tcgattaggg aatatcggat ataaaactgg aaatgtgaca
      481 ttgtcgacag aaattaaacg atatgttgtc catattccaa aaggttggac aaacgaatcc
      541 aagcagacca caaatgaatg gttgttcaat ttcaaccagg aaggaactgt ttggatttgg
      601 atgccgaagt ttgaaataag cgatgtagat acttcttatt
//
LOCUS       DAVKQB010000145          623 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVKQB010000145 DAVKQB010000000
VERSION     DAVKQB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.145"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..477
                     /locus_tag="VW528_002200"
                     /pseudo
     CDS             1..477
                     /locus_tag="VW528_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          214 a          107 c          115 g          187 t
ORIGIN      
        1 gcgctcaaag ctatgggcta cactcgaaaa aaaagaacca cacctactat gaacaagagc
       61 cagaaaaagt agccttattt cttaagaatt ttaatagttt aaagcaccta gcacctgttt
      121 agattgatga aacaggattc gatacttatt tttatcgaga atatggtcgc tcattaaaag
      181 gtcagttaat aagaggcaaa gtatctggaa gaagatatca gagaatttct ttggttgcag
      241 gtctaacaaa tgaatggtga gttaatcgct ccaatgactt acgaagagac gatgacgagc
      301 gacttttttg aagcttggtt tcagaagttt ctcttaccaa cattaaccac accatcagtt
      361 attattatgg ataatgcaag attccataga atgggtaagt tagaactctt gtgtgaagag
      421 tttgggtata aacttttacc tcttcctccc tactcacctg agtacaatcc tattgaggct
      481 ctataatatt tgtagtgggt aaatccccta tggatattat ggagcctatt tttgtgtaga
      541 aaaaaagtcc cataagatct ataatgaaaa gcgatcaaac aactcattag aaagattcat
      601 atggaacaat tacattttat cac
//
LOCUS       DAVKQB010000146          611 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVKQB010000146 DAVKQB010000000
VERSION     DAVKQB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.146"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>611
                     /locus_tag="VW528_002201"
     CDS             <1..>611
                     /locus_tag="VW528_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEU9912918.1"
                     /translation="VWIWMPKFEISDVDTSYSEAPEDIEGQISTVESTFKQRVNSLEA
                     GVNRLTEGLRTKADISALNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQ
                     EILNATKDKADKTLVVSEAGKLREEFSKMKVGGRNLWIKSKTVGAVIEKLPENHVTGQ
                     KECYRLENNSTLTFNLEPDFSSRLYQKVTFSAWIKYENVVQGR"
BASE COUNT          206 a          110 c          145 g          150 t
ORIGIN      
        1 gtttggattt ggatgccgaa gtttgaaata agcgatgtag atacttctta ttcagaagct
       61 cctgaagata tagaaggtca gatttcaaca gttgaatcga cctttaaaca acgagtcaac
      121 tcgctcgaag ctggtgtgaa tcgtctgact gaaggcctta gaaccaaagc cgatatcagc
      181 gcactcaacg tgactgctga aaatatccgg caatctgtga agagtcttga gacagacacg
      241 cagaacaagc taaatcagaa gttgagtcag gctgaatttg aggtgcgagc cggctctatc
      301 cgtcaggaaa tcctgaacgc aaccaaggat aaggcagata agactttagt tgtatctgaa
      361 gctgggaaat tgcgtgaaga attttcaaaa atgaaggtgg gaggccggaa tctatggata
      421 aaatccaaga cggttggagc tgtaattgaa aaattacctg aaaaccatgt cacaggtcaa
      481 aaagaatgtt ataggttaga gaacaactct actttaacgt tcaaccttga accagatttc
      541 agctcaaggt tgtatcaaaa agttactttt agcgcttgga tcaagtacga aaatgtagtc
      601 caaggtcgaa a
//
LOCUS       DAVKQB010000147          598 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVKQB010000147 DAVKQB010000000
VERSION     DAVKQB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.147"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            1..513
                     /locus_tag="VW528_002202"
                     /pseudo
     CDS             1..513
                     /locus_tag="VW528_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          224 a          105 c          105 g          164 t
ORIGIN      
        1 taaaccacca agtaaaagga acaaaaccaa gaaaagttga tagagataga cttaaaaact
       61 atcttactga caatccagac gcttatttga ctgaaatagc ttctgaattt ggctgtcatc
      121 caactaccat ccactatgcg ttcaaagcta tgggctacac tcgaaaaaaa gaaccacacc
      181 tactatgaac aagacccaga aaaagtagcc ttatttctta agaattttaa tagattaaag
      241 cacctagcac ctgtttagat tgacgaaaca ggattcgata cttattttta tcgagaatat
      301 ggtcgctcat taaaaggtca gttaagaaga ggcaaagtat ctggaagaag atatcagagg
      361 atttctttgg ttgcaggtct aacaaatggt gagttaatcg ctccaataac ttacgaagag
      421 acgatgacga gcgacttttt tgaagtatgg tttcagaaat ttctcttacc aacattaaac
      481 acaccatcgg ttattatagt aaaacgaaat aagaatagga caaatcgatc aggacagtaa
      541 aatcgaattc taacaatgtt ttagaagtag atgtgtacta ttctagtttc aatctact
//
LOCUS       DAVKQB010000148          549 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVKQB010000148 DAVKQB010000000
VERSION     DAVKQB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.149"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
BASE COUNT          187 a           72 c           97 g          193 t
ORIGIN      
        1 aatttgattt gaaaaatgga taaaaaaata aaggcagtcc gaagaccgcc aatatttatc
       61 tctctgcacc tttgctatgg tctttcttat ttgacttaga tttgtcatct gtcttaaagc
      121 tttttatttg ccctaatatg gactttttat ccttgtcttg actctttact tgttcttttg
      181 cttttaagag cttagaagac aaaaagagat attatattaa aacaatattt ctccggtact
      241 gggactggga cgggactaaa aatctgaaaa cgtcctataa ttaggtggat ttagtgatat
      301 tataaaaaat agaatctagt attttgaatg gttttggata tattgtatgt ttggaaaaga
      361 agaatgggag taattttaaa ctaacaaaaa atgccttgaa atcagtgttt cagagcattt
      421 ttgggttcta taatatttgt agtgggtaaa tccactatag atattatgga gcctattttg
      481 tgtagaaaaa aagtcccata tgacctataa tgaaaagcga caaaacaact cattagaaag
      541 aatcatatg
//
LOCUS       DAVKQB010000149          428 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00059224
            SAMN33606253-rid17939533.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVKQB010000149 DAVKQB010000000
VERSION     DAVKQB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606253
            Sequence Read Archive: SRR23727252
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722294.1
            Genome Coverage       :: 281x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:23:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,016
            CDSs (with protein)               :: 2,016
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 72 of 139
            Pseudo Genes (incomplete)         :: 60 of 139
            Pseudo Genes (internal stop)      :: 39 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606253-rid17939533.denovo.150"
                     /strain="AUSMDU00059224"
                     /serotype="19F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST654"
     gene            <1..>428
                     /locus_tag="VW528_002203"
     CDS             <1..>428
                     /locus_tag="VW528_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEU9912919.1"
                     /translation="LENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAES
                     VNQALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLI
                     RRWLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFL"
BASE COUNT          144 a           80 c           85 g          119 t
ORIGIN      
        1 gcttggaaaa tggtcattat gaaattgata ccgtcctact gactaagata aaaaattatt
       61 gcctgttagt cttaaccgac cggcggagca gacaccaaat tataaggtta attccaaata
      121 aaactgctga atctgtcaat caggcgctta cgttactatt aggggagcat cgtattctgt
      181 ccattactgc agataatggt tcggagttca aacgattgtc tgaggtattt cctgaggaac
      241 atatctacta cgcacatgct tactcttcat gggagagagg ttcaaatgaa aatcataatc
      301 gattaattcg gagatggtta cctaaaggaa ccaagaaaac gactccgaaa gaagtagctt
      361 ttatcgaaaa ttggattaac aactacccta aaaaatgctt ggactacaag tcgccaagtg
      421 aatttctt
//
